Cucsa.356590 (gene) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.356590
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	Matrix metalloproteinase, putative
Location	scaffold03577 : 1407356 .. 1407913 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTATCAGAAGATATCGACAACGTTAGTAGTCATGATCACCATCACCTTCCCCATGCTGTATCACATTATGCTTTCTTCCCAGGAAGGCTTAGGTGGCCATCTACCAAATATCGCCTCACATATGCGTTTCTTCCTGGCACTCGTGCAGATGCAAAAGCACCAGTGGCACGTGCGTTTGCAACATGGGCTCGAAACACACATTTTAAGTTtACTTTGGTCACAAACTATAGAAGAGCTGATTTGAAGATAGGATTTTATAGAGGAAACCATGGAGATGGGTACCCATTTGACGGACCGGGAGGAACTTTGGCACATGCTTTTGCTCCAACCGATGGGAGATTTCATTATGATTCAACAGAGAAATGGGCAGTTGGGGCAGTGAGAGGGAGATATGATTTGCAGACAGTGGCTTTGCATGAGATTGGACATCTTCTTGGACTTGGGCATAGCACAGTTAAAAATGCTATAATGTATCCTTATATAAAATCTGGGTCCACTAAGGGTTTGAATGTTGATGACATTAAAGGGATCAAGGTTTTATACAACAGACGTTAG

mRNA sequence

ATGCTATCAGAAGATATCGACAACGTTAGTAGTCATGATCACCATCACCTTCCCCATGCTGTATCACATTATGCTTTCTTCCCAGGAAGGCTTAGGTGGCCATCTACCAAATATCGCCTCACATATGCGTTTCTTCCTGGCACTCGTGCAGATGCAAAAGCACCAGTGGCACGTGCGTTTGCAACATGGGCTCGAAACACACATTTTAAGTTTACTTTGGTCACAAACTATAGAAGAGCTGATTTGAAGATAGGATTTTATAGAGGAAACCATGGAGATGGGTACCCATTTGACGGACCGGGAGGAACTTTGGCACATGCTTTTGCTCCAACCGATGGGAGATTTCATTATGATTCAACAGAGAAATGGGCAGTTGGGGCAGTGAGAGGGAGATATGATTTGCAGACAGTGGCTTTGCATGAGATTGGACATCTTCTTGGACTTGGGCATAGCACAGTTAAAAATGCTATAATGTATCCTTATATAAAATCTGGGTCCACTAAGGGTTTGAATGTTGATGACATTAAAGGGATCAAGGTTTTATACAACAGACGTTAG

Coding sequence (CDS)

ATGCTATCAGAAGATATCGACAACGTTAGTAGTCATGATCACCATCACCTTCCCCATGCTGTATCACATTATGCTTTCTTCCCAGGAAGGCTTAGGTGGCCATCTACCAAATATCGCCTCACATATGCGTTTCTTCCTGGCACTCGTGCAGATGCAAAAGCACCAGTGGCACGTGCGTTTGCAACATGGGCTCGAAACACACATTTTAAGTTtACTTTGGTCACAAACTATAGAAGAGCTGATTTGAAGATAGGATTTTATAGAGGAAACCATGGAGATGGGTACCCATTTGACGGACCGGGAGGAACTTTGGCACATGCTTTTGCTCCAACCGATGGGAGATTTCATTATGATTCAACAGAGAAATGGGCAGTTGGGGCAGTGAGAGGGAGATATGATTTGCAGACAGTGGCTTTGCATGAGATTGGACATCTTCTTGGACTTGGGCATAGCACAGTTAAAAATGCTATAATGTATCCTTATATAAAATCTGGGTCCACTAAGGGTTTGAATGTTGATGACATTAAAGGGATCAAGGTTTTATACAACAGACGTTAG

Protein sequence

MLSEDIDNVSSHDHHHLPHAVSHYAFFPGRLRWPSTKYRLTYAFLPGTRADAKAPVARAFATWARNTHFKFTLVTNYRRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDSTEKWAVGAVRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYIKSGSTKGLNVDDIKGIKVLYNRR*

Homology

[Close]

BLAST of Cucsa.356590 vs. Swiss-Prot
Match: 3MMP_ARATH (Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana GN=3MMP PE=1 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 2.4e-42
Identity = 87/175 (49.71%), Postives = 109/175 (62.29%), Query Frame = 1


Query: 19  HAVSHYAFFPGRLRWPSTKYRLTYAFLP--GTRADAKAPVARAFATWARNTHFKFTLVTN 78
           HAV HY+FFPG  RWP  +  LTYAF P      + K+  +RAF  W   T   FT V  
Sbjct: 154 HAVKHYSFFPGEPRWPRNRRDLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVER 213

Query: 79  YRRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDSTEKWAVG--------AV 138
           +  +D+ IGFY G HGDG PFDGP  TLAHAF+P  G FH D  E W V         +V
Sbjct: 214 FSTSDISIGFYSGEHGDGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISV 273

Query: 139 RGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYIKSGSTK-GLNVDDIKGIKVLY 183
               DL++VA+HEIGHLLGLGHS+V+ +IMYP I++G  K  L  DD++G++ LY
Sbjct: 274 SEAVDLESVAVHEIGHLLGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLY 328

[Close]

BLAST of Cucsa.356590 vs. Swiss-Prot
Match: 2MMP_ARATH (Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana GN=2MMP PE=1 SV=1)

HSP 1 Score: 168.7 bits (426), Expect = 6.0e-41
Identity = 86/174 (49.43%), Postives = 110/174 (63.22%), Query Frame = 1


Query: 19  HAVSHYAFFPGRLRWPSTKYRLTYAFLPGT--RADAKAPVARAFATWARNTHFKFTLVTN 78
           HAV  Y  FPG  RWP  +  LTYAF P      + K+  +RAF  W+  T   FTL  +
Sbjct: 150 HAVKRYTLFPGEPRWPRNRRDLTYAFDPKNPLTEEVKSVFSRAFGRWSDVTALNFTLSES 209

Query: 79  YRRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDSTEKWAVG-------AVR 138
           +  +D+ IGFY G+HGDG PFDG  GTLAHAF+P  G+FH D+ E W V        +V 
Sbjct: 210 FSTSDITIGFYTGDHGDGEPFDGVLGTLAHAFSPPSGKFHLDADENWVVSGDLDSFLSVT 269

Query: 139 GRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYIKSGSTK-GLNVDDIKGIKVLY 183
              DL++VA+HEIGHLLGLGHS+V+ +IMYP I +G  K  L  DD++GI+ LY
Sbjct: 270 AAVDLESVAVHEIGHLLGLGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQYLY 323

[Close]

BLAST of Cucsa.356590 vs. Swiss-Prot
Match: 5MMP_ARATH (Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana GN=5MMP PE=1 SV=1)

HSP 1 Score: 159.5 bits (402), Expect = 3.7e-38
Identity = 89/189 (47.09%), Postives = 110/189 (58.20%), Query Frame = 1


Query: 6   IDNVSSHDHHHLPHAVSHYAFFPGRLRWPSTKYRLTYAFLPGTRA--DAKAPVARAFATW 65
           ID VS  +   +      Y+FFPG+ RWP  K  LTYAF P      + K   +RAF  W
Sbjct: 125 IDGVSEMNGGKILRTTEKYSFFPGKPRWPKRKRDLTYAFAPQNNLTDEVKRVFSRAFTRW 184

Query: 66  ARNTHFKFTLVTNYRRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDSTEKW 125
           A  T   FT   +  RAD+ IGF+ G HGDG PFDG  GTLAHA +P  G  H D  E W
Sbjct: 185 AEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAMGTLAHASSPPTGMLHLDGDEDW 244

Query: 126 AV--GAVRGR-------YDLQTVALHEIGHLLGLGHSTVKNAIMYPYIKSGSTK-GLNVD 183
            +  G +  R        DL++VA+HEIGHLLGLGHS+V++AIM+P I  G  K  L  D
Sbjct: 245 LISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSSVEDAIMFPAISGGDRKVELAKD 304

[Close]

BLAST of Cucsa.356590 vs. Swiss-Prot
Match: MEP1_SOYBN (Metalloendoproteinase 1 OS=Glycine max PE=1 SV=2)

HSP 1 Score: 158.3 bits (399), Expect = 8.1e-38
Identity = 85/171 (49.71%), Postives = 104/171 (60.82%), Query Frame = 1


Query: 21  VSHYAFFPGRLRWPSTKYRLTYAFLPGTRADA--KAPVARAFATWARNTHFKFTLVTNYR 80
           +S Y FF    RW +   +LTYAF P  R D   K+ +ARAF+ W    +  F   T+Y 
Sbjct: 131 ISDYTFFKDMPRWQAGTTQLTYAFSPEPRLDDTFKSAIARAFSKWTPVVNIAFQETTSYE 190

Query: 81  RADLKIGFYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDSTEKWAVGA------VRGRY 140
            A++KI F   NHGD YPFDGPGG L HAFAPTDGR H+D+ E W          V   +
Sbjct: 191 TANIKILFASKNHGDPYPFDGPGGILGHAFAPTDGRCHFDADEYWVASGDVTKSPVTSAF 250

Query: 141 DLQTVALHEIGHLLGLGHSTVKNAIMYPYIKSGSTK-GLNVDDIKGIKVLY 183
           DL++VA+HEIGHLLGLGHS+   AIMYP I   + K  L  DDI GI+ LY
Sbjct: 251 DLESVAVHEIGHLLGLGHSSDLRAIMYPSIPPRTRKVNLAQDDIDGIRKLY 301

[Close]

BLAST of Cucsa.356590 vs. Swiss-Prot
Match: 1MMP_ARATH (Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana GN=1MMP PE=1 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 2.2e-35
Identity = 81/176 (46.02%), Postives = 105/176 (59.66%), Query Frame = 1


Query: 19  HAVSHYAFFPGRLRWPSTKYRLTYAFLPG------TRADAKAPVARAFATWARNTHFKFT 78
           H  +HY +F G+ +W   +  LTYA          T  D K    RAF+ W+      F 
Sbjct: 145 HTTAHYTYFNGKPKW--NRDTLTYAISKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSFE 204

Query: 79  LVTNYRRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDSTEKWAV-----GA 138
            V ++  ADLKIGFY G+HGDG PFDG  GTLAHAFAP +GR H D+ E W V     G+
Sbjct: 205 EVDDFTTADLKIGFYAGDHGDGLPFDGVLGTLAHAFAPENGRLHLDAAETWIVDDDLKGS 264

Query: 139 VRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYIKSGSTK-GLNVDDIKGIKVLY 183
                DL++VA HEIGHLLGLGHS+ ++A+MYP ++  + K  L VDD+ G+  LY
Sbjct: 265 SEVAVDLESVATHEIGHLLGLGHSSQESAVMYPSLRPRTKKVDLTVDDVAGVLKLY 318

[Close]

BLAST of Cucsa.356590 vs. TrEMBL
Match: A0A0A0M0J6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G654860 PE=3 SV=1)

HSP 1 Score: 393.7 bits (1010), Expect = 1.3e-106
Identity = 185/185 (100.00%), Postives = 185/185 (100.00%), Query Frame = 1


Query: 1   MLSEDIDNVSSHDHHHLPHAVSHYAFFPGRLRWPSTKYRLTYAFLPGTRADAKAPVARAF 60
           MLSEDIDNVSSHDHHHLPHAVSHYAFFPGRLRWPSTKYRLTYAFLPGTRADAKAPVARAF
Sbjct: 130 MLSEDIDNVSSHDHHHLPHAVSHYAFFPGRLRWPSTKYRLTYAFLPGTRADAKAPVARAF 189

Query: 61  ATWARNTHFKFTLVTNYRRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDST 120
           ATWARNTHFKFTLVTNYRRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDST
Sbjct: 190 ATWARNTHFKFTLVTNYRRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDST 249

Query: 121 EKWAVGAVRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYIKSGSTKGLNVDDIKGIKV 180
           EKWAVGAVRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYIKSGSTKGLNVDDIKGIKV
Sbjct: 250 EKWAVGAVRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYIKSGSTKGLNVDDIKGIKV 309

Query: 181 LYNRR 186
           LYNRR
Sbjct: 310 LYNRR 314

[Close]

BLAST of Cucsa.356590 vs. TrEMBL
Match: A0A0A0M032_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G654840 PE=3 SV=1)

HSP 1 Score: 317.4 bits (812), Expect = 1.2e-83
Identity = 150/184 (81.52%), Postives = 162/184 (88.04%), Query Frame = 1


Query: 1   MLSEDIDNVSSHDHHH-LPHAVSHYAFFPGRLRWPSTKYRLTYAFLPGTRADAKAPVARA 60
           ML E+ +N SSHDH+H L HAV HY+FFPGR RWP TKY LTY FLP T ADAKAPV RA
Sbjct: 128 MLLENNNNDSSHDHYHQLSHAVPHYSFFPGRPRWPPTKYHLTYEFLPNTHADAKAPVTRA 187

Query: 61  FATWARNTHFKFTLVTNYRRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDS 120
           FATWAR+THFKF+L TN RRADLKIGFYRGNHGDGYPFDG GGTLAHAF PTDGR H+DS
Sbjct: 188 FATWARHTHFKFSLATNSRRADLKIGFYRGNHGDGYPFDGSGGTLAHAFTPTDGRVHFDS 247

Query: 121 TEKWAVGAVRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYIKSGSTKGLNVDDIKGIK 180
           TEKW VGAVRGR+DL+TVALHEIGHLLGLGHS VKNAIMYP I+SGSTKGLN DDI+GI+
Sbjct: 248 TEKWVVGAVRGRFDLETVALHEIGHLLGLGHSRVKNAIMYPTIESGSTKGLNADDIEGIE 307

Query: 181 VLYN 184
           VLYN
Sbjct: 308 VLYN 311

[Close]

BLAST of Cucsa.356590 vs. TrEMBL
Match: B9RUG6_RICCO (Metalloendoproteinase 1, putative OS=Ricinus communis GN=RCOM_0852690 PE=3 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 3.8e-66
Identity = 119/177 (67.23%), Postives = 141/177 (79.66%), Query Frame = 1


Query: 10  SSHDHHHLP---HAVSHYAFFPGRLRWPSTKYRLTYAFLPGTRADAKAPVARAFATWARN 69
           + + HHH     H VSHY FFPG  RWP++KY LTY FLPGT   A  PVA+AF TWA N
Sbjct: 134 NKNPHHHSSTSFHTVSHYEFFPGNPRWPASKYHLTYGFLPGTPNQAMEPVAKAFQTWAAN 193

Query: 70  THFKFTLVTNYRRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDSTEKWAVG 129
           THF+FT V +YR AD+ IGF+RG+HGDG PFDG GGTLAHAFAP DGRFHYD  E WAVG
Sbjct: 194 THFRFTRVQDYRAADITIGFHRGDHGDGSPFDGRGGTLAHAFAPQDGRFHYDGDEHWAVG 253

Query: 130 AVRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYIKSGSTKGLNVDDIKGIKVLYN 184
           A +G +D++TVALHEIGHLLGLGHS+V+ AIM+P I+SG+TKGL+ DDI+GI+ LYN
Sbjct: 254 ATQGAFDVETVALHEIGHLLGLGHSSVEGAIMHPSIQSGATKGLHSDDIQGIRALYN 310

[Close]

BLAST of Cucsa.356590 vs. TrEMBL
Match: S1SMU3_THECC (Matrixin family protein OS=Theobroma cacao GN=TCM_044196 PE=3 SV=1)

HSP 1 Score: 247.3 bits (630), Expect = 1.5e-62
Identity = 117/166 (70.48%), Postives = 133/166 (80.12%), Query Frame = 1


Query: 19  HAVSHYAFFPGRLRWPSTKYRLTYAFLPGTRADAKAPVARAFATWARNTHFKFTLVTNYR 78
           H VSHYAFFP   RWP +K  LTYAFLPGTRADA  PVA AF TWA NTHF+F+ + NYR
Sbjct: 146 HEVSHYAFFPRSPRWPPSKSHLTYAFLPGTRADAVNPVAGAFQTWAANTHFRFSRIDNYR 205

Query: 79  RADLKIGFYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDSTEKWAVGAVRGRYDLQTVA 138
            AD+ IGF R +HGDG PFDGPGGTLAHAFAPT GRFHYD+ E W+V A  G   L+TVA
Sbjct: 206 DADITIGFQRRDHGDGNPFDGPGGTLAHAFAPTLGRFHYDADETWSVSARPGTMHLETVA 265

Query: 139 LHEIGHLLGLGHSTVKNAIMYPYIKSGSTKGLNVDDIKGIKVLYNR 185
           LHEIGHLLGLGHS+++NAIMYP I +G++KGL  DDI+GIK LYNR
Sbjct: 266 LHEIGHLLGLGHSSIENAIMYPSITAGTSKGLARDDIEGIKALYNR 311

[Close]

BLAST of Cucsa.356590 vs. TrEMBL
Match: A0A061FPL5_THECC (Matrixin family protein OS=Theobroma cacao GN=TCM_044201 PE=3 SV=1)

HSP 1 Score: 245.4 bits (625), Expect = 5.6e-62
Identity = 116/166 (69.88%), Postives = 132/166 (79.52%), Query Frame = 1


Query: 19  HAVSHYAFFPGRLRWPSTKYRLTYAFLPGTRADAKAPVARAFATWARNTHFKFTLVTNYR 78
           H VSHYAFFP   RWP +K  LTYAFLPGTRADA  PVA AF TWA NTHF+F+ + NYR
Sbjct: 146 HEVSHYAFFPRSPRWPPSKSHLTYAFLPGTRADAVNPVAGAFQTWAANTHFRFSRIDNYR 205

Query: 79  RADLKIGFYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDSTEKWAVGAVRGRYDLQTVA 138
            AD+ IGF R +HGDG PFDGPGGTLAHAFAPT GRFHYD+ E W+V A  G   L+TVA
Sbjct: 206 DADITIGFQRRDHGDGNPFDGPGGTLAHAFAPTIGRFHYDADETWSVSARPGTMHLETVA 265

Query: 139 LHEIGHLLGLGHSTVKNAIMYPYIKSGSTKGLNVDDIKGIKVLYNR 185
           LHEIGHLLGL HS+++NAIMYP I +G++KGL  DDI+GIK LYNR
Sbjct: 266 LHEIGHLLGLSHSSIENAIMYPSITAGTSKGLARDDIEGIKALYNR 311

[Close]

BLAST of Cucsa.356590 vs. TAIR10
Match: AT1G24140.1 (AT1G24140.1 Matrixin family protein)

HSP 1 Score: 173.3 bits (438), Expect = 1.4e-43
Identity = 87/175 (49.71%), Postives = 109/175 (62.29%), Query Frame = 1


Query: 19  HAVSHYAFFPGRLRWPSTKYRLTYAFLP--GTRADAKAPVARAFATWARNTHFKFTLVTN 78
           HAV HY+FFPG  RWP  +  LTYAF P      + K+  +RAF  W   T   FT V  
Sbjct: 154 HAVKHYSFFPGEPRWPRNRRDLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVER 213

Query: 79  YRRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDSTEKWAVG--------AV 138
           +  +D+ IGFY G HGDG PFDGP  TLAHAF+P  G FH D  E W V         +V
Sbjct: 214 FSTSDISIGFYSGEHGDGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISV 273

Query: 139 RGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYIKSGSTK-GLNVDDIKGIKVLY 183
               DL++VA+HEIGHLLGLGHS+V+ +IMYP I++G  K  L  DD++G++ LY
Sbjct: 274 SEAVDLESVAVHEIGHLLGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLY 328

[Close]

BLAST of Cucsa.356590 vs. TAIR10
Match: AT1G70170.1 (AT1G70170.1 matrix metalloproteinase)

HSP 1 Score: 168.7 bits (426), Expect = 3.4e-42
Identity = 86/174 (49.43%), Postives = 110/174 (63.22%), Query Frame = 1


Query: 19  HAVSHYAFFPGRLRWPSTKYRLTYAFLPGT--RADAKAPVARAFATWARNTHFKFTLVTN 78
           HAV  Y  FPG  RWP  +  LTYAF P      + K+  +RAF  W+  T   FTL  +
Sbjct: 150 HAVKRYTLFPGEPRWPRNRRDLTYAFDPKNPLTEEVKSVFSRAFGRWSDVTALNFTLSES 209

Query: 79  YRRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDSTEKWAVG-------AVR 138
           +  +D+ IGFY G+HGDG PFDG  GTLAHAF+P  G+FH D+ E W V        +V 
Sbjct: 210 FSTSDITIGFYTGDHGDGEPFDGVLGTLAHAFSPPSGKFHLDADENWVVSGDLDSFLSVT 269

Query: 139 GRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYIKSGSTK-GLNVDDIKGIKVLY 183
              DL++VA+HEIGHLLGLGHS+V+ +IMYP I +G  K  L  DD++GI+ LY
Sbjct: 270 AAVDLESVAVHEIGHLLGLGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQYLY 323

[Close]

BLAST of Cucsa.356590 vs. TAIR10
Match: AT1G59970.1 (AT1G59970.1 Matrixin family protein)

HSP 1 Score: 159.5 bits (402), Expect = 2.1e-39
Identity = 89/189 (47.09%), Postives = 110/189 (58.20%), Query Frame = 1


Query: 6   IDNVSSHDHHHLPHAVSHYAFFPGRLRWPSTKYRLTYAFLPGTRA--DAKAPVARAFATW 65
           ID VS  +   +      Y+FFPG+ RWP  K  LTYAF P      + K   +RAF  W
Sbjct: 125 IDGVSEMNGGKILRTTEKYSFFPGKPRWPKRKRDLTYAFAPQNNLTDEVKRVFSRAFTRW 184

Query: 66  ARNTHFKFTLVTNYRRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDSTEKW 125
           A  T   FT   +  RAD+ IGF+ G HGDG PFDG  GTLAHA +P  G  H D  E W
Sbjct: 185 AEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAMGTLAHASSPPTGMLHLDGDEDW 244

Query: 126 AV--GAVRGR-------YDLQTVALHEIGHLLGLGHSTVKNAIMYPYIKSGSTK-GLNVD 183
            +  G +  R        DL++VA+HEIGHLLGLGHS+V++AIM+P I  G  K  L  D
Sbjct: 245 LISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSSVEDAIMFPAISGGDRKVELAKD 304

[Close]

BLAST of Cucsa.356590 vs. TAIR10
Match: AT4G16640.1 (AT4G16640.1 Matrixin family protein)

HSP 1 Score: 150.2 bits (378), Expect = 1.2e-36
Identity = 81/176 (46.02%), Postives = 105/176 (59.66%), Query Frame = 1


Query: 19  HAVSHYAFFPGRLRWPSTKYRLTYAFLPG------TRADAKAPVARAFATWARNTHFKFT 78
           H  +HY +F G+ +W   +  LTYA          T  D K    RAF+ W+      F 
Sbjct: 145 HTTAHYTYFNGKPKW--NRDTLTYAISKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSFE 204

Query: 79  LVTNYRRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDSTEKWAV-----GA 138
            V ++  ADLKIGFY G+HGDG PFDG  GTLAHAFAP +GR H D+ E W V     G+
Sbjct: 205 EVDDFTTADLKIGFYAGDHGDGLPFDGVLGTLAHAFAPENGRLHLDAAETWIVDDDLKGS 264

Query: 139 VRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYIKSGSTK-GLNVDDIKGIKVLY 183
                DL++VA HEIGHLLGLGHS+ ++A+MYP ++  + K  L VDD+ G+  LY
Sbjct: 265 SEVAVDLESVATHEIGHLLGLGHSSQESAVMYPSLRPRTKKVDLTVDDVAGVLKLY 318

[Close]

BLAST of Cucsa.356590 vs. TAIR10
Match: AT2G45040.1 (AT2G45040.1 Matrixin family protein)

HSP 1 Score: 149.4 bits (376), Expect = 2.1e-36
Identity = 82/176 (46.59%), Postives = 102/176 (57.95%), Query Frame = 1


Query: 19  HAVSHYAFFPGRLRWP-STKYRLTYAFLPGTRADAKAPV------ARAFATWARNTHFKF 78
           H    Y +FPGR RW      +LTYAF         AP        RAF  WA      F
Sbjct: 120 HTGKKYVYFPGRPRWTRDVPLKLTYAFSQENLTPYLAPTDIRRVFRRAFGKWASVIPVSF 179

Query: 79  TLVTNYRRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDSTEKWAVGAVRGR 138
               +Y  AD+KIGF+ G+HGDG PFDG  G LAH F+P +GR H D  E WAV     +
Sbjct: 180 IETEDYVIADIKIGFFNGDHGDGEPFDGVLGVLAHTFSPENGRLHLDKAETWAVDFDEEK 239

Query: 139 ----YDLQTVALHEIGHLLGLGHSTVKNAIMYPYIKSGSTK-GLNVDDIKGIKVLY 183
                DL++VA+HEIGH+LGLGHS+VK+A MYP +K  S K  LN+DD+ G++ LY
Sbjct: 240 SSVAVDLESVAVHEIGHVLGLGHSSVKDAAMYPTLKPRSKKVNLNMDDVVGVQSLY 295

[Close]

BLAST of Cucsa.356590 vs. NCBI nr
Match: gi|449442791|ref|XP_004139164.1| (PREDICTED: metalloendoproteinase 1-like [Cucumis sativus])

HSP 1 Score: 393.7 bits (1010), Expect = 1.8e-106
Identity = 185/185 (100.00%), Postives = 185/185 (100.00%), Query Frame = 1


Query: 1   MLSEDIDNVSSHDHHHLPHAVSHYAFFPGRLRWPSTKYRLTYAFLPGTRADAKAPVARAF 60
           MLSEDIDNVSSHDHHHLPHAVSHYAFFPGRLRWPSTKYRLTYAFLPGTRADAKAPVARAF
Sbjct: 130 MLSEDIDNVSSHDHHHLPHAVSHYAFFPGRLRWPSTKYRLTYAFLPGTRADAKAPVARAF 189

Query: 61  ATWARNTHFKFTLVTNYRRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDST 120
           ATWARNTHFKFTLVTNYRRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDST
Sbjct: 190 ATWARNTHFKFTLVTNYRRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDST 249

Query: 121 EKWAVGAVRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYIKSGSTKGLNVDDIKGIKV 180
           EKWAVGAVRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYIKSGSTKGLNVDDIKGIKV
Sbjct: 250 EKWAVGAVRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYIKSGSTKGLNVDDIKGIKV 309

Query: 181 LYNRR 186
           LYNRR
Sbjct: 310 LYNRR 314

[Close]

BLAST of Cucsa.356590 vs. NCBI nr
Match: gi|659085906|ref|XP_008443669.1| (PREDICTED: metalloendoproteinase 1-like [Cucumis melo])

HSP 1 Score: 372.9 bits (956), Expect = 3.4e-100
Identity = 173/185 (93.51%), Postives = 179/185 (96.76%), Query Frame = 1


Query: 1   MLSEDIDNVSSHDHHHLPHAVSHYAFFPGRLRWPSTKYRLTYAFLPGTRADAKAPVARAF 60
           MLSE  +N SSHDHHHLPHAVSHYAFFPGR RWPSTKYRLTYAFLPGTRADAKAPV RAF
Sbjct: 129 MLSEHNNNDSSHDHHHLPHAVSHYAFFPGRRRWPSTKYRLTYAFLPGTRADAKAPVTRAF 188

Query: 61  ATWARNTHFKFTLVTNYRRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDST 120
           ATWARNTHFKF+L+TNYRRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDST
Sbjct: 189 ATWARNTHFKFSLITNYRRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDST 248

Query: 121 EKWAVGAVRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYIKSGSTKGLNVDDIKGIKV 180
           EKWAVGAV+GRYDLQTVALHEIGHLLGLGHSTV+NAIMYPYI+SGSTKGLN DDIKGIKV
Sbjct: 249 EKWAVGAVKGRYDLQTVALHEIGHLLGLGHSTVRNAIMYPYIRSGSTKGLNADDIKGIKV 308

Query: 181 LYNRR 186
           LYNRR
Sbjct: 309 LYNRR 313

[Close]

BLAST of Cucsa.356590 vs. NCBI nr
Match: gi|449442789|ref|XP_004139163.1| (PREDICTED: metalloendoproteinase 1-like [Cucumis sativus])

HSP 1 Score: 317.4 bits (812), Expect = 1.7e-83
Identity = 150/184 (81.52%), Postives = 162/184 (88.04%), Query Frame = 1


Query: 1   MLSEDIDNVSSHDHHH-LPHAVSHYAFFPGRLRWPSTKYRLTYAFLPGTRADAKAPVARA 60
           ML E+ +N SSHDH+H L HAV HY+FFPGR RWP TKY LTY FLP T ADAKAPV RA
Sbjct: 128 MLLENNNNDSSHDHYHQLSHAVPHYSFFPGRPRWPPTKYHLTYEFLPNTHADAKAPVTRA 187

Query: 61  FATWARNTHFKFTLVTNYRRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDS 120
           FATWAR+THFKF+L TN RRADLKIGFYRGNHGDGYPFDG GGTLAHAF PTDGR H+DS
Sbjct: 188 FATWARHTHFKFSLATNSRRADLKIGFYRGNHGDGYPFDGSGGTLAHAFTPTDGRVHFDS 247

Query: 121 TEKWAVGAVRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYIKSGSTKGLNVDDIKGIK 180
           TEKW VGAVRGR+DL+TVALHEIGHLLGLGHS VKNAIMYP I+SGSTKGLN DDI+GI+
Sbjct: 248 TEKWVVGAVRGRFDLETVALHEIGHLLGLGHSRVKNAIMYPTIESGSTKGLNADDIEGIE 307

Query: 181 VLYN 184
           VLYN
Sbjct: 308 VLYN 311

[Close]

BLAST of Cucsa.356590 vs. NCBI nr
Match: gi|255552736|ref|XP_002517411.1| (PREDICTED: metalloendoproteinase 3-MMP [Ricinus communis])

HSP 1 Score: 259.2 bits (661), Expect = 5.4e-66
Identity = 119/177 (67.23%), Postives = 141/177 (79.66%), Query Frame = 1


Query: 10  SSHDHHHLP---HAVSHYAFFPGRLRWPSTKYRLTYAFLPGTRADAKAPVARAFATWARN 69
           + + HHH     H VSHY FFPG  RWP++KY LTY FLPGT   A  PVA+AF TWA N
Sbjct: 134 NKNPHHHSSTSFHTVSHYEFFPGNPRWPASKYHLTYGFLPGTPNQAMEPVAKAFQTWAAN 193

Query: 70  THFKFTLVTNYRRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDSTEKWAVG 129
           THF+FT V +YR AD+ IGF+RG+HGDG PFDG GGTLAHAFAP DGRFHYD  E WAVG
Sbjct: 194 THFRFTRVQDYRAADITIGFHRGDHGDGSPFDGRGGTLAHAFAPQDGRFHYDGDEHWAVG 253

Query: 130 AVRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYIKSGSTKGLNVDDIKGIKVLYN 184
           A +G +D++TVALHEIGHLLGLGHS+V+ AIM+P I+SG+TKGL+ DDI+GI+ LYN
Sbjct: 254 ATQGAFDVETVALHEIGHLLGLGHSSVEGAIMHPSIQSGATKGLHSDDIQGIRALYN 310

[Close]

BLAST of Cucsa.356590 vs. NCBI nr
Match: gi|645249911|ref|XP_008230961.1| (PREDICTED: metalloendoproteinase 1-like [Prunus mume])

HSP 1 Score: 253.4 bits (646), Expect = 3.0e-64
Identity = 114/171 (66.67%), Postives = 137/171 (80.12%), Query Frame = 1


Query: 13  DHHHLPHAVSHYAFFPGRLRWPSTKYRLTYAFLPGTRADAKAPVARAFATWARNTHFKFT 72
           +HHH  H V+HYAFFPG+ +WP++KY LTYAFL GT   AK PVARAF TWA +T FKF+
Sbjct: 140 NHHHKGHTVAHYAFFPGKPKWPASKYHLTYAFLRGTPTAAKGPVARAFNTWAAHTQFKFS 199

Query: 73  LVTNYRRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDSTEKWAVGAVRGRY 132
                ++ DL + F+R +HGDG+PFDGPGG LAH+FAP DGRFHYD+ EKW+VG VRG +
Sbjct: 200 QAPRNQKPDLTVSFHRRSHGDGHPFDGPGGILAHSFAPKDGRFHYDADEKWSVGVVRGAF 259

Query: 133 DLQTVALHEIGHLLGLGHSTVKNAIMYPYIKSGSTKGLNVDDIKGIKVLYN 184
            L+TVALHEIGHLLGLGHS+V+ AIMYP I SG TKGLN DDI+GIK LY+
Sbjct: 260 HLETVALHEIGHLLGLGHSSVRRAIMYPTISSGETKGLNRDDIQGIKALYH 310

The following BLAST results are available for this feature:

BLAST of Cucsa.356590 vs. Swiss-Prot


Match Name	E-value	Identity	Description
3MMP_ARATH	2.4e-42	49.71	Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana GN=3MMP PE=1 SV=1	[more]
2MMP_ARATH	6.0e-41	49.43	Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana GN=2MMP PE=1 SV=1	[more]
5MMP_ARATH	3.7e-38	47.09	Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana GN=5MMP PE=1 SV=1	[more]
MEP1_SOYBN	8.1e-38	49.71	Metalloendoproteinase 1 OS=Glycine max PE=1 SV=2	[more]
1MMP_ARATH	2.2e-35	46.02	Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana GN=1MMP PE=1 SV=1	[more]

BLAST of Cucsa.356590 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0M0J6_CUCSA	1.3e-106	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G654860 PE=3 SV=1	[more]
A0A0A0M032_CUCSA	1.2e-83	81.52	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G654840 PE=3 SV=1	[more]
B9RUG6_RICCO	3.8e-66	67.23	Metalloendoproteinase 1, putative OS=Ricinus communis GN=RCOM_0852690 PE=3 SV=1	[more]
S1SMU3_THECC	1.5e-62	70.48	Matrixin family protein OS=Theobroma cacao GN=TCM_044196 PE=3 SV=1	[more]
A0A061FPL5_THECC	5.6e-62	69.88	Matrixin family protein OS=Theobroma cacao GN=TCM_044201 PE=3 SV=1	[more]

BLAST of Cucsa.356590 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G24140.1	1.4e-43	49.71	Matrixin family protein	[more]
AT1G70170.1	3.4e-42	49.43	matrix metalloproteinase	[more]
AT1G59970.1	2.1e-39	47.09	Matrixin family protein	[more]
AT4G16640.1	1.2e-36	46.02	Matrixin family protein	[more]
AT2G45040.1	2.1e-36	46.59	Matrixin family protein	[more]

BLAST of Cucsa.356590 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449442791\|ref\|XP_004139164.1\|	1.8e-106	100.00	PREDICTED: metalloendoproteinase 1-like [Cucumis sativus]	[more]
gi\|659085906\|ref\|XP_008443669.1\|	3.4e-100	93.51	PREDICTED: metalloendoproteinase 1-like [Cucumis melo]	[more]
gi\|449442789\|ref\|XP_004139163.1\|	1.7e-83	81.52	PREDICTED: metalloendoproteinase 1-like [Cucumis sativus]	[more]
gi\|255552736\|ref\|XP_002517411.1\|	5.4e-66	67.23	PREDICTED: metalloendoproteinase 3-MMP [Ricinus communis]	[more]
gi\|645249911\|ref\|XP_008230961.1\|	3.0e-64	66.67	PREDICTED: metalloendoproteinase 1-like [Prunus mume]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR001818	Pept_M10_metallopeptidase
IPR006026	Peptidase_Metallo
IPR021190	Pept_M10A
IPR024079	MetalloPept_cat_dom_sf

Vocabulary: **Molecular Function**
Term	Definition
GO:0004222	metalloendopeptidase activity
GO:0008270	zinc ion binding
GO:0008237	metallopeptidase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006508	proteolysis

Vocabulary: **Cellular Component**
Term	Definition
GO:0031012	extracellular matrix

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0031012	extracellular matrix
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0004222	metalloendopeptidase activity
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0008237	metallopeptidase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsa.356590.1	Cucsa.356590.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001818	Peptidase M10, metallopeptidase	PFAM	PF00413	Peptidase_M10	coord: 37..182 score: 9.0
IPR006026	Peptidase, metallopeptidase	SMART	SM00235	col_5	coord: 29..184 score: 7.4
IPR021190	Peptidase M10A	PRINTS	PR00138	MATRIXIN	coord: 80..108 score: 3.8E-31coord: 137..162 score: 3.8E-31coord: 56..71 score: 3.8E-31coord: 170..183 score: 3.8
IPR024079	Metallopeptidase, catalytic domain	GENE3D	G3DSA:3.40.390.10		coord: 21..184 score: 5.7
None	No IPR available	PANTHER	PTHR10201	MATRIX METALLOPROTEINASE	coord: 19..182 score: 3.1
None	No IPR available	PANTHER	PTHR10201:SF140	F20P5.11 PROTEIN	coord: 19..182 score: 3.1
None	No IPR available	unknown	SSF55486	Metalloproteases ("zincins"), catalytic domain	coord: 26..183 score: 2.4

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cucsa.356590	Cucurbita pepo (Zucchini)	cgycpeB0927
Cucsa.356590	Cucurbita pepo (Zucchini)	cgycpeB0928
Cucsa.356590	Bottle gourd (USVL1VR-Ls)	cgylsiB573
Cucsa.356590	Melon (DHL92) v3.6.1	cgymedB575
Cucsa.356590	Silver-seed gourd	carcgyB0613
Cucsa.356590	Silver-seed gourd	carcgyB1085
Cucsa.356590	Cucumber (Chinese Long) v3	cgycucB542
Cucsa.356590	Watermelon (97103) v2	cgywmbB608
Cucsa.356590	Wax gourd	cgywgoB709
Cucsa.356590	Wax gourd	cgywgoB711
Cucsa.356590	Cucurbita maxima (Rimu)	cgycmaB0969
Cucsa.356590	Cucurbita maxima (Rimu)	cgycmaB0970
Cucsa.356590	Cucurbita moschata (Rifu)	cgycmoB0972
Cucsa.356590	Cucurbita moschata (Rifu)	cgycmoB0973
Cucsa.356590	Wild cucumber (PI 183967)	cgycpiB528
Cucsa.356590	Cucumber (Chinese Long) v2	cgycuB500
Cucsa.356590	Melon (DHL92) v3.5.1	cgymeB574
Cucsa.356590	Watermelon (Charleston Gray)	cgywcgB605
Cucsa.356590	Watermelon (97103) v1	cgywmB638