Cucsa.356500 (gene) Cucumber (Gy14) v1

Overview
NameCucsa.356500
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionLINE-1 reverse transcriptase like
Locationscaffold03577 : 1306933 .. 1308688 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTCAATCAAAACAATTGGCTATTAAAACACTTTTGAAGAAAGTTAATCCAGATATAGTAATGATTGAAGAGACCAAAAGAGAAGAAATTGACAGTTGTATTATAAAGGCACTATGGAGTTCGAAAGGAATCGGCTGGGACCTAGTTGAAGCTAATGGCAGATCAGGAGGATTGCTGATTGTGTGGGATGAATCTAAGCAGTCAGTTTTGGAAGTACTGAAACGAGGATATTCTATTTCAATAAAATGCAGCACAATCAATAGAAAAATCTGTGCTGGATCTCAAACATCTATGGACCCACACATTATCGAGATAGAAACCAAATTGGTCGGAGTTGTCTCTTCTGTCAGACCTCTGTACAGGAGCTTGGTGTTTGGGTGGGGATTTCAACATTACTCGGAAGGTACAAGAGAGATTTCCAGTACGAAGATTAACAAGGGGAATGAGAAAATTTAATAAATTCATTAGAGAGTCAAAATTGCTAGAAATTCCGCTGTCCAATGGTAAATTCTCATGGTCAAGAGAAGGAAGGACAGTTTCACGTTCTCTGCTGGATAGGTTCCTAGTAACTGATGATTGGGATGAATCTTTTGTTGATACCAGAGCCTCTCAAAAGGAAAGACTTGCTTCAGATCATTTCCCCATCTTATTAGAATTCGGTGCTTTTGAATGGGGCCCCTCTCCATTTCGCTTTTGCAACAGCTGGCTGCTCGATAAGCAGTGCTGCCATATTATAAAAAaTTCTTTGACCTCAGGAAATCATCAAGGGTGGGCTGGTTTTGTCATTTGTTCAAAACTACGAAATTTAAAAAGCTCCTTGAAATCTTGGCTTGTGGACAGTGAAAGAGCCAGAAAAAaCCAAGAAGAGTCTTTGCTCCAAGGTCTTGAAAATGAAGAAATTAAGGAAGAATCTCAGACCCTGTCATCCTTAGACAACATTATGAATTGTCTATCAAATCAGACCTCTTAGCAATCTATAGAAGAGAGGAAAGAGACTTAATTCAAAAATGTAAGTTGAACTGGTTAAAATTGGGAGATGAAAATACTAGTTTCGTCCATCGTTTCCTTGCTGCAAAAAAAaGGCATAGCTTGATTTCAGAATTGATTGATGACCAAGGTACTCCTACAGTTTCATTTAATGGAATTGAAAGTCTCATCATTGAATTCTACAAGTCTCTCTATACAAAAGCCCCTCAAGCTTGGCTCTCTTCCTAATCCCCTTGAATGGTCAGTGGTTTCGGCAGAACAAAATACAAGGCTCACCTCAATATTTAGTCTGCCCGAAATAAAATCTGCACTTCAACTCCTGGGAAAAAATAAGGCCCCTGGACCAGACAGGTATACAGCTGAATTTCTAATCAGCTTTTGGGATCATTTCAAAGATAATTACCGTGCTCTCTTTAATGAGTTTTATAAGAATGGGAGGTTAAATGCATGTGTAAAAGAGAACTTCATTTGTTTAATCAAGAAGAAAGAAGATGCAGTAAGGGTAAAAGACTTTAGACCAATAAGTCTTACAACATCAATTTATAAGCTGGTCGCAAAGGTTCTCGCTGAAAGATTAAAGAAAGTAATGCCAAGTATTATTGCTCCATCACAAAGTGGCTTTCTGGAAGGGCGCCAAATCTTGGACCCTATTTTAATTGCAAACAAAGCTGTGGAGGATTATAGAATCAGAAAAAaGAAAGGATGGATCATTAAATTAGACCTTGAAAAGGCTTTCGACATAGTAGATTGGGCATTTTTAGAAAAA

mRNA sequence

atgggtcaatcaaaacaattggctattaaaacacttttgaagaaagttaatccagatatagtaatgattgaagagaccaaaagagaagaaattgacagttgtattataaaggcactatggagttcgaaaggaatcggctgggacctagttgaagctaatggcagatcaggaggattgctgattgtgtgggatgaatctaagcagtcagttttggaaaaaccaaattggtcggagttgtctcttctgtcagacctctgtacaggagcttggtgtttgggtggggatttcaacattactcggaaggtacaagagagatttccagtacgaagattaacaaggggaatgagaaaatttaataaattcattagagagtcaaaattgctagaaattccgctgtccaatggtaaattctcatggtcaagagaaggaaggacagtttcacgttctctgctggataggttcctagtaactgatgattgggatgaatcttttgttgataccagagcctctcaaaaggaaagacttgcttcagatcatttccccatcttattagaattcggtgcttttgaatggggcccctctccatttcgcttttgcaacagctggctgctcgataagcagtgctgccatattataaaaaattctttgacctcaggaaatcatcaagggtgggctggttttgtcatttgttcaaaactacgaaatttaaaaagctccttgaaatcttggcttgtggacagtgaaagagccagaaaaaaccaagaagagtctttgctccaagaccctgtcatccttagacaacattatgaattgtctatcaaatcagacctcttagcaatctatagaagagaggaaagagacttaattcaaaaatgtaagttgaactggttaaaattgggagatgaaaatactagtttcgtccatcgtttccttgctgcaaaaaaaaggcatagcttgatttcagaattgattgatgaccaagtggtttcggcagaacaaaatacaaggctcacctcaatatttagtctgcccgaaataaaatctgcacttcaactcctgggaaaaaataaggcccctggaccagacaggtatacagctgaatttctaatcagcttttgggatcatttcaaagataattaccgtgctctctttaatgagttttataagaatgggaggttaaatgcatgtgtaaaagagaacttcatttgtttaatcaagaagaaagaagatgcagtaagggtaaaagactttagaccaataagtcttacaacatcaatttataagctggtcgcaaaggttctcgctgaaagattaaagaaagtaatgccaagtattattgctccatcacaaagtggctttctggaagggcgccaaatcttggaccctattttaattgcaaacaaagctgtggaggattatagaatcagaaaaaagaaaggatggatcattaaattagaccttgaaaaggctttcgacatagtagattgggcatttttagaaaaa

Coding sequence (CDS)

ATGGGTCAATCAAAACAATTGGCTATTAAAACACTTTTGAAGAAAGTTAATCCAGATATAGTAATGATTGAAGAGACCAAAAGAGAAGAAATTGACAGTTGTATTATAAAGGCACTATGGAGTTCGAAAGGAATCGGCTGGGACCTAGTTGAAGCTAATGGCAGATCAGGAGGATTGCTGATTGTGTGGGATGAATCTAAGCAGTCAGTTTTGGAAAAACCAAATTGGTCGGAGTTGTCTCTTCTGTCAGACCTCTGTACAGGAGCTTGGTGTTTGGGTGGGGATTTCAACATTACTCGGAAGGTACAAGAGAGATTTCCAGTACGAAGATTAACAAGGGGAATGAGAAAATTTAATAAATTCATTAGAGAGTCAAAATTGCTAGAAATTCCGCTGTCCAATGGTAAATTCTCATGGTCAAGAGAAGGAAGGACAGTTTCACGTTCTCTGCTGGATAGGTTCCTAGTAACTGATGATTGGGATGAATCTTTTGTTGATACCAGAGCCTCTCAAAAGGAAAGACTTGCTTCAGATCATTTCCCCATCTTATTAGAATTCGGTGCTTTTGAATGGGGCCCCTCTCCATTTCGCTTTTGCAACAGCTGGCTGCTCGATAAGCAGTGCTGCCATATTATAAAAAaTTCTTTGACCTCAGGAAATCATCAAGGGTGGGCTGGTTTTGTCATTTGTTCAAAACTACGAAATTTAAAAAGCTCCTTGAAATCTTGGCTTGTGGACAGTGAAAGAGCCAGAAAAAaCCAAGAAGAGTCTTTGCTCCAAGACCCTGTCATCCTTAGACAACATTATGAATTGTCTATCAAATCAGACCTCTTAGCAATCTATAGAAGAGAGGAAAGAGACTTAATTCAAAAATGTAAGTTGAACTGGTTAAAATTGGGAGATGAAAATACTAGTTTCGTCCATCGTTTCCTTGCTGCAAAAAAAaGGCATAGCTTGATTTCAGAATTGATTGATGACCAAGTGGTTTCGGCAGAACAAAATACAAGGCTCACCTCAATATTTAGTCTGCCCGAAATAAAATCTGCACTTCAACTCCTGGGAAAAAATAAGGCCCCTGGACCAGACAGGTATACAGCTGAATTTCTAATCAGCTTTTGGGATCATTTCAAAGATAATTACCGTGCTCTCTTTAATGAGTTTTATAAGAATGGGAGGTTAAATGCATGTGTAAAAGAGAACTTCATTTGTTTAATCAAGAAGAAAGAAGATGCAGTAAGGGTAAAAGACTTTAGACCAATAAGTCTTACAACATCAATTTATAAGCTGGTCGCAAAGGTTCTCGCTGAAAGATTAAAGAAAGTAATGCCAAGTATTATTGCTCCATCACAAAGTGGCTTTCTGGAAGGGCGCCAAATCTTGGACCCTATTTTAATTGCAAACAAAGCTGTGGAGGATTATAGAATCAGAAAAAaGAAAGGATGGATCATTAAATTAGACCTTGAAAAGGCTTTCGACATAGTAGATTGGGCATTTTTAGAAAAA

Protein sequence

MGQSKQLAIKTLLKKVNPDIVMIEETKREEIDSCIIKALWSSKGIGWDLVEANGRSGGLLIVWDESKQSVLEKPNWSELSLLSDLCTGAWCLGGDFNITRKVQERFPVRRLTRGMRKFNKFIRESKLLEIPLSNGKFSWSREGRTVSRSLLDRFLVTDDWDESFVDTRASQKERLASDHFPILLEFGAFEWGPSPFRFCNSWLLDKQCCHIIKNSLTSGNHQGWAGFVICSKLRNLKSSLKSWLVDSERARKNQEESLLQDPVILRQHYELSIKSDLLAIYRREERDLIQKCKLNWLKLGDENTSFVHRFLAAKKRHSLISELIDDQVVSAEQNTRLTSIFSLPEIKSALQLLGKNKAPGPDRYTAEFLISFWDHFKDNYRALFNEFYKNGRLNACVKENFICLIKKKEDAVRVKDFRPISLTTSIYKLVAKVLAERLKKVMPSIIAPSQSGFLEGRQILDPILIANKAVEDYRIRKKKGWIIKLDLEKAFDIVDWAFLEK
Homology
[Close]
BLAST of Cucsa.356500 vs. Swiss-Prot
Match: YTX2_XENLA (Transposon TX1 uncharacterized 149 kDa protein OS=Xenopus laevis PE=3 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 1.2e-22
Identity = 62/172 (36.05%), Postives = 93/172 (54.07%), Query Frame = 1 

Query: 328 VVSAEQNTRLTSIFSLPEIKSALQLLGKNKAPGPDRYTAEFLISFWDHFKDNYRALFNEF 387
           VVS  +  RL +  +L E+  AL+L+  NK+PG D  T EF   FWD    ++  +  E 
Sbjct: 436 VVSERRKERLETPITLDELSQALRLMPHNKSPGLDGLTIEFFQFFWDTLGPDFHRVLTEA 495

Query: 388 YKNGRLNACVKENFICLIKKKEDAVRVKDFRPISLTTSIYKLVAKVLAERLKKVMPSIIA 447
           +K G L    +   + L+ KK D   +K++RP+SL ++ YK+VAK ++ RLK V+  +I 
Sbjct: 496 FKKGELPLSCRRAVLSLLPKKGDLRLIKNWRPVSLLSTDYKIVAKAISLRLKSVLAEVIH 555

Query: 448 PSQSGFLEGRQILDPILIANKAVEDYRIRKKKGWIIKLDLEKAFDIVDWAFL 500
           P QS  + GR I D + +    +   R        + LD EKAFD VD  +L
Sbjct: 556 PDQSYTVPGRTIFDNVFLIRDLLHFARRTGLSLAFLSLDQEKAFDRVDHQYL 607

[Close]
BLAST of Cucsa.356500 vs. Swiss-Prot
Match: LORF2_MOUSE (LINE-1 retrotransposable element ORF2 protein OS=Mus musculus GN=Pol PE=1 SV=2)

HSP 1 Score: 85.5 bits (210), Expect = 1.8e-15
Identity = 60/181 (33.15%), Postives = 95/181 (52.49%), Query Frame = 1 

Query: 329 VSAEQNTRLTSIFSLPEIKSALQLLGKNKAPGPDRYTAEFLISFWDHFKDNYRALFNEFY 388
           ++ +Q   L S  S  EI++ +  L   K+PGPD ++AEF  +F +        LF++  
Sbjct: 448 LNQDQVDHLNSPISPKEIEAVINSLPTKKSPGPDGFSAEFYQTFKEDLIPILHKLFHKIE 507

Query: 389 KNGRLNACVKENFICLIKK-KEDAVRVKDFRPISLTTSIYKLVAKVLAERLKKVMPSIIA 448
             G L     E  I LI K ++D  ++++FRPISL     K++ K+LA R+++ + +II 
Sbjct: 508 VEGTLPNSFYEATITLIPKPQKDPTKIENFRPISLMNIDAKILNKILANRIQEHIKAIIH 567

Query: 449 PSQSGFLEG-------RQILDPILIANKAVEDYRIRKKKGWIIKLDLEKAFDIVDWAFLE 502
           P Q GF+ G       R+ ++ I   NK      ++ K   II LD EKAFD +   F+ 
Sbjct: 568 PDQVGFIPGMQGWFNIRKSINVIHYINK------LKDKNHMIISLDAEKAFDKIQHPFMI 622

[Close]
BLAST of Cucsa.356500 vs. Swiss-Prot
Match: LIN1_NYCCO (LINE-1 reverse transcriptase homolog OS=Nycticebus coucang PE=3 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 9.9e-14
Identity = 54/173 (31.21%), Postives = 87/173 (50.29%), Query Frame = 1 

Query: 329 VSAEQNTRLTSIFSLPEIKSALQLLGKNKAPGPDRYTAEFLISFWDHFKDNYRALFNEFY 388
           +S ++   L    S  EI S +Q L K K+PGPD +T+EF  +F +        LF    
Sbjct: 440 LSQKEVEMLNRPISSSEIASTIQNLPKKKSPGPDGFTSEFYQTFKEELVPILLNLFQNIE 499

Query: 389 KNGRL-NACVKENFICLIKKKEDAVRVKDFRPISLTTSIYKLVAKVLAERLKKVMPSIIA 448
           K G L N   + N   + K  +D  R +++RPISL     K++ K+L  R+++ +  II 
Sbjct: 500 KEGILPNTFYEANITLIPKPGKDPTRKENYRPISLMNIDAKILNKILTNRIQQHIKKIIH 559

Query: 449 PSQSGFLEGRQILDPILIANKAVEDY-RIRKKKGWIIKLDLEKAFDIVDWAFL 500
             Q GF+ G Q    I  +   ++   +++ K   I+ +D EKAFD +   F+
Sbjct: 560 HDQVGFIPGSQGWFNIRKSINVIQHINKLKNKDHMILSIDAEKAFDNIQHPFM 612

[Close]
BLAST of Cucsa.356500 vs. Swiss-Prot
Match: LORF2_HUMAN (LINE-1 retrotransposable element ORF2 protein OS=Homo sapiens PE=1 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 4.2e-12
Identity = 51/159 (32.08%), Postives = 80/159 (50.31%), Query Frame = 1 

Query: 345 EIKSALQLLGKNKAPGPDRYTAEFLISFWDHFKDNYRALFNEFYKNGRL-NACVKENFIC 404
           EI + +  L   K+PGPD +TAEF   + +        LF    K G L N+  + + I 
Sbjct: 457 EIVAIINSLPTKKSPGPDGFTAEFYQRYKEELVPFLLKLFQSIEKEGILPNSFYEASIIL 516

Query: 405 LIKKKEDAVRVKDFRPISLTTSIYKLVAKVLAERLKKVMPSIIAPSQSGFLEGRQILDPI 464
           + K   D  + ++FRPISL     K++ K+LA R+++ +  +I   Q GF+ G Q    I
Sbjct: 517 IPKPGRDTTKKENFRPISLMNIDAKILNKILANRIQQHIKKLIHHDQVGFIPGMQGWFNI 576

Query: 465 LIANKAVEDY-RIRKKKGWIIKLDLEKAFDIVDWAFLEK 502
             +   ++   R + K   II +D EKAFD +   F+ K
Sbjct: 577 RKSINVIQHINRAKDKNHVIISIDAEKAFDKIQQPFMLK 615

[Close]
BLAST of Cucsa.356500 vs. TrEMBL
Match: M5WJ76_PRUPE (Uncharacterized protein (Fragment) OS=Prunus persica GN=PRUPE_ppa015871mg PE=4 SV=1)

HSP 1 Score: 287.0 bits (733), Expect = 4.5e-74
Identity = 167/447 (37.36%), Postives = 246/447 (55.03%), Query Frame = 1 

Query: 76  WSELSLLSDLCTGAWCLGGDFNITRKVQERFPVRRLTRGMRKFNKFIRESKLLEIPLSNG 135
           W EL+ L   C   WCLGGDFN+ R   E+    R+T+ MR FN FI+E+ L +  L N 
Sbjct: 443 WEELADLYGYCGDMWCLGGDFNVVRFSAEKSNEGRVTKSMRDFNDFIQETNLRDPILLNA 502

Query: 136 KFSWSREGRTVSRSLLDRFLVTDDWDESFVDTRASQKERLASDHFPILLEFGAFEWGPSP 195
            F+WS          LDRFLV+  W+E F   R     R+ SDH PI L+    +WGPSP
Sbjct: 503 SFTWSNLRENAVCRRLDRFLVSGSWEEHFPHYRHKALPRITSDHCPIELDTSRVKWGPSP 562

Query: 196 FRFCNSWLLDKQCCHIIKNSLTSGNHQGWAGFVICSKLRNLKSSLKSW----LVDSERAR 255
           FRF N WL        IK         GW G+   ++L+ LKS LK W      D ER  
Sbjct: 563 FRFENMWLNHPDFKRKIKLWWGEDQIPGWEGYKFMTRLKMLKSKLKVWSKEEFGDVERDL 622

Query: 256 KNQEESLLQ----------DPVILRQHYELSIKSDLLAIYRREERDLIQKCKLNWLKLGD 315
           +  E  LL           D ++  +   L +K   LA  ++EE    Q+ K+ W + GD
Sbjct: 623 REAEARLLVLDQREGTEGLDHLLRSERDNLLLKIGDLA--QKEEVKWRQRGKVKWARDGD 682

Query: 316 ENTSFVHRFLAAKKRHSLISEL-IDDQ-VVSAEQNTR------LTSIFSLPEIKSALQLL 375
            NT F HR     ++ + I +L ++D  V+  + N           ++S  + K+     
Sbjct: 683 GNTKFFHRVANGARKRNYIEKLEVEDLGVIEVDANIEREVIRFFKGLYSSNKNKAVFDC- 742

Query: 376 GKNKAPGPDRYTAEFLISFWDHFKDNYRALFNEFYKNGRLNACVKENFICLIKKKEDAVR 435
           GK+K+PGPD ++  F  S W+  K +   +  +F+++G +N    E FICLI KK ++V+
Sbjct: 743 GKDKSPGPDGFSMSFFQSCWEVVKGDLMKVMQDFFQSGIVNGVTNETFICLIPKKANSVK 802

Query: 436 VKDFRPISLTTSIYKLVAKVLAERLKKVMPSIIAPSQSGFLEGRQILDPILIANKAVEDY 495
           V D+RPISL TS+YK+++KVLA  L++V+ + I+ SQ  F++ RQILD +L+AN+ VE+ 
Sbjct: 803 VTDYRPISLVTSLYKVISKVLASSLREVLGNTISQSQGAFVQKRQILDAVLVANEVVEEV 862

Query: 496 RIRKKKGWIIKLDLEKAFDIVDWAFLE 501
           R +K+KG + K+D EKA+D V+W F++
Sbjct: 863 RKQKRKGLVFKIDFEKAYDHVEWNFVD 886

[Close]
BLAST of Cucsa.356500 vs. TrEMBL
Match: M5WJ76_PRUPE (Uncharacterized protein (Fragment) OS=Prunus persica GN=PRUPE_ppa015871mg PE=4 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 3.4e-05
Identity = 24/68 (35.29%), Postives = 44/68 (64.71%), Query Frame = 1 

Query: 5   KQLAIKTLLKKVNPDIVMIEETKREEIDSCIIKALWSSKGIGWDLVEANGRSGGLLIVWD 64
           K+L +K  L+++ PDIV++ ETK+E +D  ++  +W S+   W    + GRSGG+ ++W+
Sbjct: 338 KRLLVKEQLRRLKPDIVILLETKKEIVDRQLVAGVWGSRFKEWVFSPSLGRSGGIAVLWN 397

Query: 65  ESKQSVLE 73
               SV++
Sbjct: 398 SQSVSVID 405


HSP 2 Score: 282.0 bits (720), Expect = 1.5e-72
Identity = 191/583 (32.76%), Postives = 283/583 (48.54%), Query Frame = 1 

Query: 5   KQLAIKTLLKKVNPDIVMIEETKREEI-----------------------DSCIIKALWS 64
           K+L +K  L+++ PDIV++ ETK+E +                        S  I  LW+
Sbjct: 2   KRLLVKEQLRRLKPDIVILLETKKETVDRQLVAGVWGSRFKEWVFSPSLGRSGGIAVLWN 61

Query: 65  SKGIGW------------DLVEANGRSGGLLIVWDESKQSVLEKPNWSELSLLSDLCTGA 124
           S+ +               +VE  G    L  ++   +Q       W EL+ L   C   
Sbjct: 62  SQSVSVIDSMVGEFSVSIRIVENIGTDWWLSGIYGPCRQRE-RNSFWEELADLYGFCGDK 121

Query: 125 WCLGGDFNITRKVQERFPVRRLTRGMRKFNKFIRESKLLEIPLSNGKFSWSREGRTVSRS 184
           WCLGGDFN+ R   E+    R+T+ MR FN FI+E+ L +  L N  F+WS         
Sbjct: 122 WCLGGDFNVVRFSAEKSNEGRVTKSMRDFNDFIQETNLRDPNLLNASFTWSNLRENAVCR 181

Query: 185 LLDRFLVTDDWDESFVDTRASQKERLASDHFPILLEFGAFEWGPSPFRFCNSWLLDKQCC 244
            LDRFLV+  W++ F   R     R+ SDH PI L+    +WGPSPFRF N WL      
Sbjct: 182 RLDRFLVSGSWEDHFPHYRHKALPRITSDHCPIELDTSRVKWGPSPFRFENMWLNHPDFM 241

Query: 245 HIIKNSLTSGNHQGWAGFVICSKLRNLKSSLKSW----LVDSERARKNQEESLLQ----- 304
             IK         GW G+   ++L+ LKS LK W      D ER  +  E  LL      
Sbjct: 242 RKIKLWWGEDQIPGWEGYKFMTRLKMLKSKLKVWSKEEFGDVERDLREAEARLLVLDQRE 301

Query: 305 -----DPVILRQHYELSIKSDLLAIYRREERDLIQKCKLNWLKLGDENTSF--------- 364
                D ++  +   L +K   LA  ++EE    Q+ K+ W + GD NT F         
Sbjct: 302 GTEGLDHLLRSERDNLLLKIGDLA--QKEEVKWRQRGKVKWAREGDGNTKFFHRVANGAR 361

Query: 365 -------------------------VHRFL----AAKKRHSLISELIDDQVVSAEQNTRL 424
                                    V RF     ++ K      E ++   +S  +   L
Sbjct: 362 KRNYIEKLEVEDLGVIEVDANIEREVIRFFKGLYSSNKNVGWGVEGLNWCPISQVEADWL 421

Query: 425 TSIFSLPEIKSALQLLGKNKAPGPDRYTAEFLISFWDHFKDNYRALFNEFYKNGRLNACV 484
              F L E++ A+   GK+K+PGPD ++  F  S W+  K +   +  +F+++G +N   
Sbjct: 422 ERPFDLEEVQKAVFECGKDKSPGPDGFSMSFFQSCWEVVKGDLMKVMQDFFQSGIVNGVT 481

Query: 485 KENFICLIKKKEDAVRVKDFRPISLTTSIYKLVAKVLAERLKKVMPSIIAPSQSGFLEGR 501
            E FICLI KK ++V+V D RPISL TS+YK+++KVLA RL++V+ + I+ SQ  F++ R
Sbjct: 482 NETFICLIPKKANSVKVTDNRPISLVTSLYKVISKVLASRLREVLGNTISQSQGAFVQKR 541

[Close]
BLAST of Cucsa.356500 vs. TrEMBL
Match: A5BSG1_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_010061 PE=4 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 2.1e-71
Identity = 170/477 (35.64%), Postives = 239/477 (50.10%), Query Frame = 1 

Query: 76   WSELSLLSDLCTGAWCLGGDFNITRKVQERFPVRRLTRGMRKFNKFIRESKLLEIPLSNG 135
            W EL  +  L +  WC+GGDFN+ R   ER    RLT  MR+F++ I E  L ++PL  G
Sbjct: 678  WEELGAIRGLWSDPWCIGGDFNVVRFPSERSREGRLTGSMRRFSEVIEELXLKDLPLHGG 737

Query: 136  KFSWSREGRTVSRSLLDRFLVTDDWDESFVDTRASQKERLASDHFPILLEFGAFEWGPSP 195
             F+WS     +SRS LDRFL+++DW+  F         R  SDHFPILL+ G    GP P
Sbjct: 738  LFTWSGGLNGLSRSRLDRFLISEDWENHFSGAXQCSLPRPVSDHFPILLDGGGTRRGPIP 797

Query: 196  FRFCNSWLLDKQCCHIIKNSLTSGNHQGWAGFVICSKLRNLKSSLKSW------------ 255
            FRF N WL ++    ++K      N+ G   F++  KL+ LK  LK W            
Sbjct: 798  FRFENMWLKEEGFKELLKGWWQGFNYSGSYSFILTEKLKALKIKLKEWNSEVFGKVGVNK 857

Query: 256  --LVDSERARKNQEE--SLLQDPVILRQHYELSIKSDLLAIYRREERDLIQKCKLNWLKL 315
               +D      NQE+  +L +  +  R+      K   L     EE    QK +  WLK 
Sbjct: 858  GLALDKVSYWDNQEQLRALNEQELEARKEAREEFKKWALM----EETSWRQKSREIWLKE 917

Query: 316  GDENTSFVHRFLAAKKRHSLIS------------------------ELIDD--------- 375
            GD+NT F H+   + +R + +                         +L+ D         
Sbjct: 918  GDKNTGFFHKMXNSNRRRNCLKKIKVNGTWLSEDHDIQRGVVRAFXDLLSDPGGWRPCXN 977

Query: 376  ----QVVSAEQNTRLTSIFSLPEIKSALQLLGKNKAPGPDRYTAEFLISFWDHFKDNYRA 435
                  +  E+  RL   FS  E+  AL  L  +KAPGPD ++  F    WD  KD    
Sbjct: 978  NIEFDSIGDEEAARLEESFSGDEVFLALSDLNGDKAPGPDGFSLAFWQFCWDFVKDEVLG 1037

Query: 436  LFNEFYKNGRLNACVKENFICLIKKKEDAVRVKDFRPISLTTSIYKLVAKVLAERLKKVM 495
             F +FY+ G+    +   F+ LI KK  A  + DFRPISL   +YKL+AKVLA RLKKV+
Sbjct: 1038 FFKDFYERGKFVRSLNSTFLVLIPKKCGAEDLXDFRPISLVGGLYKLLAKVLANRLKKVV 1097

Query: 496  PSIIAPSQSGFLEGRQILDPILIANKAVEDYRIRKKKGWIIKLDLEKAFDIVDWAFL 500
              +++ +Q+ F+EGRQILD  L+AN+ ++    RK+ G + KLDLEKA+D ++W FL
Sbjct: 1098 GKVVSSTQNAFVEGRQILDAALVANEVIDSVLKRKESGVLCKLDLEKAYDQINWDFL 1150

[Close]
BLAST of Cucsa.356500 vs. TrEMBL
Match: A5BSG1_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_010061 PE=4 SV=1)

HSP 1 Score: 175.3 bits (443), Expect = 1.9e-40
Identity = 112/372 (30.11%), Postives = 169/372 (45.43%), Query Frame = 1 

Query: 3   QSKQLAIKTLLKKVNPDIVMIEETKREEIDSCIIKALWSSKGIGWDLVEANGRSGGLLIV 62
           + K+  IK L++    D+V + ETK ++I   II +L   + +GW  + A G +G +++ 
Sbjct: 570 RDKRKVIKALIRSQKADLVCLXETKIQDISRXIIHSLGVGRFLGWGAMSARGAAGRVVVF 629

Query: 63  WDESKQSVLEKP-----------------------------------NWSELSLLSDLCT 122
           WD     ++                                       W EL  +  L +
Sbjct: 630 WDXRVLELMGMEVGLFSVSCRFKNCXDGFLWTFTGVYGPTMKRHREXFWEELGAIRGLWS 689

Query: 123 GAWCLGGDFNITRKVQERFPVRRLTRGMRKFNKFIRESKLLEIPLSNGKFSWSREGRTVS 182
             WC+GGDFN+ R   ER    RLT  MR+F++ I E  L ++PL  G F+WS     +S
Sbjct: 690 DPWCIGGDFNVVRFPSERSREGRLTGSMRRFSEVIEELXLKDLPLHGGLFTWSGGLNGLS 749

Query: 183 RSLLDRFLVTDDWDESFVDTRASQKERLASDHFPILLEFGAFEWGPSPFRFCNSWLLDKQ 242
           RS LDRFL+++DW+  F         R  SDHFPILL+ G    GP PFRF N WL ++ 
Sbjct: 750 RSRLDRFLISEDWENHFSGAXQCSLPRPVSDHFPILLDGGGTRRGPIPFRFENMWLKEEG 809

Query: 243 CCHIIKNSLTSGNHQGWAGFVICSKLRNLKSSLKSW--------------LVDSERARKN 302
              ++K      N+ G   F++  KL+ LK  LK W               +D      N
Sbjct: 810 FKELLKGWWQGFNYSGSYSFILTEKLKALKIKLKEWNSEVFGKVGVNKGLALDKVSYWDN 869

Query: 303 QEE--SLLQDPVILRQHYELSIKSDLLAIYRREERDLIQKCKLNWLKLGDENTSFVHRFL 324
           QE+  +L +  +  R+      K   L     EE    QK +  WLK GD+NT F H+  
Sbjct: 870 QEQLRALNEQELEARKEAREEFKKWAL----MEETSWRQKSREIWLKEGDKNTGFFHKMX 929


HSP 2 Score: 144.4 bits (363), Expect = 3.6e-31
Identity = 97/270 (35.93%), Postives = 147/270 (54.44%), Query Frame = 1 

Query: 234  RNLKSS-LKSWLVDSERARKNQ-EESLLQDPVILRQHYELSIKSDLLAIYRRE------- 293
            +N+K   L S L     +RK + EE +L++ +  RQ  +   K   +A  RR        
Sbjct: 1655 KNIKEGGLNSDLXSQRASRKGELEELILREEIHWRQKAKFYHK---VANGRRNRKYIKEL 1714

Query: 294  --ERDLIQKCKLNWLKLGDENTSFVHRFLAAKKRHSLISELIDDQVVSAEQNTRLTSIFS 353
              ER L+ K   N   + +E   +  +   +  R S   E +D   +S E   RL S F+
Sbjct: 1715 ENERGLVLK---NAESITEEILHYFEKLYTSPTRESWGVEGLDWSPISEESALRLDSPFT 1774

Query: 354  LPEIKSALQLLGKNKAPGPDRYTAEFLISFWDHFKDNYRALFNEFYKNGRLNACVKENFI 413
              EI   +  L ++KAPGPD +T       WD  K+    +F EF+++G +N     +FI
Sbjct: 1775 EEEISKTIFQLDRDKAPGPDGFTIAVFQECWDVIKEELVRVFAEFHRSGIINQSTNASFI 1834

Query: 414  CLIKKKEDAVRVKDFRPISLTTSIYKLVAKVLAERLKKVMPSIIAPSQSGFLEGRQILDP 473
             L+ KK  + R+ DFRPISL TS+YK++AKVL+ RL+ V+   I  +Q  F++GRQILD 
Sbjct: 1835 VLLPKKSLSKRISDFRPISLITSLYKIIAKVLSGRLRGVLHETIHYTQGAFVQGRQILDA 1894

Query: 474  ILIANKAVEDYRIRKKKGWIIKLDLEKAFD 493
            +LIAN+ V++ R   ++G I K+D EKA+D
Sbjct: 1895 VLIANEIVDERRRSGEEGVIFKIDFEKAYD 1918


HSP 3 Score: 272.3 bits (695), Expect = 1.2e-69
Identity = 173/435 (39.77%), Postives = 236/435 (54.25%), Query Frame = 1 

Query: 76   WSELSLLSDLCTGAWCLGGDFNITRKVQERFPVRRLTRGMRKFNKFIRESKLLEIPLSNG 135
            W EL  +  L +  WC+GGDFN+ R  +ER     L+  MR+F + + + +L + PL  G
Sbjct: 772  WEELGSIKGLWSDPWCVGGDFNMIRYPEERRRGGELSASMRRFTEVVEDLELRDYPLQGG 831

Query: 136  KFSWSREGRTVSRSLLDRFLVTDDWDESFVDTRASQKERLASDHFPILLEFGAFEWGPSP 195
             F+W       S+S LDRFLVTD+WD  F   +     R  SDHFPILLE G  + GPSP
Sbjct: 832  LFTWRGGLNNQSQSRLDRFLVTDEWDRMFNGAKQGILARPVSDHFPILLEGGGLKRGPSP 891

Query: 196  FRFCNSWLLDKQCCHIIKNSLTSGNHQGWAGFV---ICSKLRNLKSSLKSWLVDSERARK 255
            FRF N WL ++     +K +L  G  + W   V   I +K R     +  W      A +
Sbjct: 892  FRFENMWLEERGFMDQLKRAL-KGLLKTWNKEVFGAIETKKREALXQVAXW-----DAVE 951

Query: 256  NQEESLLQDPVILRQHYELSIKSDLLAIYRREERDLIQKCKLNWLKLGDENTSFVHRFLA 315
            N     L+D    ++  E + K+ +L    REE    Q+ +  WLK GD NT F HR   
Sbjct: 952  NHSTLSLEDCEARKEAKE-AYKTWVL----REEISWRQRSRELWLKEGDNNTKFFHRMAN 1011

Query: 316  AKKRHSLISELIDDQVVSAEQNTRLTSI-------FSLPEIKSALQLLGKNKAPGPDRYT 375
            A  R + +S L  D     E+     S+       F+  E+  AL  LGK+KAPGPD +T
Sbjct: 1012 AHSRRNWLSRLKVDDCWHTEELDLKNSVAVGLEIPFTEGEVXVALSDLGKDKAPGPDGFT 1071

Query: 376  AEFLISFWDHFKDNYRALFNEFYKNGRLNACVKENFICLIKKKEDAVRVKDFRPISLTTS 435
              F    WD  K      F EF + G     +   F+ L+ K+  A  +KDFRPISL  S
Sbjct: 1072 MAFWSFSWDLVKXXIMGFFKEFXERGXFVKSLNATFLVLVPKRGGAEDLKDFRPISLVGS 1131

Query: 436  IYKLVAKVLAERLKKVMPSIIAPSQSGFLEGRQILDPILIANKAVEDYRIRKKKGWII-K 495
            +YKL+ KVLA R+KKVM  +I+ SQ+ F+EGRQILD +LIAN+AV D R++   G ++ K
Sbjct: 1132 LYKLLVKVLANRIKKVMGKVISESQNAFVEGRQILDAVLIANEAV-DSRLKDNVGGVLXK 1191

Query: 496  LDLEKAFDIVDWAFL 500
            LD+EKA+D V W+FL
Sbjct: 1192 LDIEKAYDRVSWSFL 1194

[Close]
BLAST of Cucsa.356500 vs. TrEMBL
Match: A5AN19_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_031023 PE=4 SV=1)

HSP 1 Score: 38.1 bits (87), Expect = 3.7e+01
Identity = 23/87 (26.44%), Postives = 44/87 (50.57%), Query Frame = 1 

Query: 10  KTLLKKVNPDIVMIE--ETKREEIDSCIIKALWSSKGIGWDLVEANGRSGGLLIVWDESK 69
           K+ + K + ++  +E  ETK +++   I+++L   + I W  + + G +GG+L+ WD   
Sbjct: 669 KSSVTKSSRELKKLEWTETKIKDMSMGIVRSLGVGRHIDWRAINSKGAAGGVLVFWDNRV 728

Query: 70  QSVLEKPNWSELSLLSDLCTGAWCLGG 95
             +LE     E  + S  C    C+ G
Sbjct: 729 VDLLE----VEEGIFSVSCLFKNCMDG 751


HSP 2 Score: 272.3 bits (695), Expect = 1.2e-69
Identity = 162/475 (34.11%), Postives = 237/475 (49.89%), Query Frame = 1 

Query: 76  WSELSLLSDLCTGAWCLGGDFNITRKVQERFPVRRLTRGMRKFNKFIRESKLLEIPLSNG 135
           W EL ++  L +  WC+GGDFN+ +   ER    R+T  MR+F++ I E  L ++PL  G
Sbjct: 23  WEELGVIRGLWSDPWCIGGDFNVVKFPSERSIEXRITGSMRRFSEVIEELALRDLPLHGG 82

Query: 136 KFSWSREGRTVSRSLLDRFLVTDDWDESFVDTRASQKERLASDHFPILLEFGAFEWGPSP 195
            F+W+     +S S LDRFL++++W+  F         R  SDHFPILL+ G    GP+P
Sbjct: 83  XFTWTGGLNGLSXSRLDRFLISEEWENHFSGVFXCSLPRPVSDHFPILLDGGXVRRGPTP 142

Query: 196 FRFCNSWLLDKQCCHIIKNSLTSGNHQGWAGFVICSKLRNLKSSLKSW------------ 255
           F F N WL +     ++K      N+ G   F++  KL+ LK  LK W            
Sbjct: 143 FXFENMWLKEDXFKDLLKGWWQGFNYSGSYSFILTKKLKALKIKLKEWNSEVFGKVGVNK 202

Query: 256 --LVDSERARKNQEESLLQDPVILRQHYELSIKSDLLAIYRREERDLIQKCKLNWLKLGD 315
              +D      NQE   + +   L    E   K D       EE    QK +  WLK GD
Sbjct: 203 RLALDKVSYWDNQERLRVLNDWELEXRKEA--KEDFKKWVLMEEISWRQKSRQIWLKEGD 262

Query: 316 ENTSFVHRFLAAKKRHSLISEL-------------------------------------I 375
           +NT F H+   + +R + + ++                                     +
Sbjct: 263 KNTXFFHKMXNSNRRKNCLKKIKVNGTWLSEEQDIQRGVVRAFQSLLSDPXGWRPSVNNL 322

Query: 376 DDQVVSAEQNTRLTSIFSLPEIKSALQLLGKNKAPGPDRYTAEFLISFWDHFKDNYRALF 435
           +   + AE+  RL  +F++ E+  AL  L  +KAPG D +T  F    WD  KD     F
Sbjct: 323 EFDSIGAEEAARLELMFTVEEVFLALSELNGDKAPGXDGFTLAFWHFCWDFVKDEIMGFF 382

Query: 436 NEFYKNGRLNACVKENFICLIKKKEDAVRVKDFRPISLTTSIYKLVAKVLAERLKKVMPS 495
            +F++ G+    +   F+ LI KK  A  + DF PISL  S+YKL+AKVLA RLKKV+  
Sbjct: 383 KDFFERGKFVRSLNTTFLVLIPKKGGAEDLTDFXPISLVGSLYKLLAKVLANRLKKVVGK 442

Query: 496 IIAPSQSGFLEGRQILDPILIANKAVEDYRIRKKKGWIIKLDLEKAFDIVDWAFL 500
           +++ +Q+ F+EGRQILD  LIAN+ ++    RK+ G + KLDLEKA+D ++W FL
Sbjct: 443 VVSTTQNAFVEGRQILDAALIANEVIDSLLKRKESGVLCKLDLEKAYDXINWDFL 495

[Close]
BLAST of Cucsa.356500 vs. TAIR10
Match: AT1G43760.1 (AT1G43760.1 DNAse I-like superfamily protein)

HSP 1 Score: 101.3 bits (251), Expect = 1.8e-21
Identity = 116/454 (25.55%), Postives = 183/454 (40.31%), Query Frame = 1 

Query: 57  GGLLIVWDESKQSVLEKPNWSELSLLSDLCTGAWCLGGDFNITRKVQERFPVRRLT---R 116
           G + IVWD S  SVL      +L +L           GDF+      + + V + +   R
Sbjct: 199 GRIWIVWDPSV-SVLVFKKTDQLMILV----------GDFDQIAATSDHYSVLQTSIPMR 258

Query: 117 GMRKFNKFIRESKLLEIPLSNGKFSWS--REGRTVSRSLLDRFLVTDDWDESFVDTRASQ 176
           G+ +F   +R+S L++IP     ++WS  ++   + R L DR +   DW  SF    A  
Sbjct: 259 GLEEFQNCLRDSDLVDIPSRGVHYTWSNHQDDNPIIRKL-DRAIANGDWFSSFPSAIAVF 318

Query: 177 KERLASDHFP--ILLE---------FGAFEW---------------------GPSPFRFC 236
           +    SDH P  I+LE         F  F +                     G   F   
Sbjct: 319 ELSGVSDHSPCIIILENLPKRSKKCFRYFSFLSTHPTFLVSLTVAWEEQIPVGSHMFSLG 378

Query: 237 NSWLLDKQCCHIIKNSLTSGNHQGWAGFVICSKLRNLKSSLKSWLVDSERARKNQEESLL 296
                 K+CC ++       N QG+    I  K +    SL+S      +   N  +SL 
Sbjct: 379 EHLKAAKKCCKLL-------NRQGFGN--IQHKTKEALDSLESI---QSQLLTNPSDSLF 438

Query: 297 QDPVILRQHYELSIKSDLLAIYRREERDLIQKCKLNWLKLGDENTSFVHRFLAAKKRHSL 356
           +   + R+ +     + L + YR       QK ++ WL+ GD NT F H+ + A +  +L
Sbjct: 439 RVEHVARKKWNF-FAAALESFYR-------QKSRIKWLQDGDANTRFFHKVILANQAKNL 498

Query: 357 ISEL-IDDQV-------------------VSAEQN-----------------------TR 416
           I  L +DD V                   + ++ +                       +R
Sbjct: 499 IKFLRMDDDVRVENVTQVKEMIVAYYTHLLGSDSDILTPDSVQRIKDIHPFRCNDTLASR 558

Query: 417 LTSIFSLPEIKSALQLLGKNKAPGPDRYTAEFLISFWDHFKDNYRALFNEFYKNGRLNAC 431
           L+++ S  EI +A+  + +NKAPGPD +TAEF    W   KD+  A   EF++ G L   
Sbjct: 559 LSALPSDKEITAAVFAMPRNKAPGPDSFTAEFFWESWFVVKDSTIAAVKEFFRTGHLLKR 618

[Close]
BLAST of Cucsa.356500 vs. TAIR10
Match: AT4G20520.1 (AT4G20520.1 RNA binding;RNA-directed DNA polymerases)

HSP 1 Score: 60.8 bits (146), Expect = 2.7e-09
Identity = 31/69 (44.93%), Postives = 46/69 (66.67%), Query Frame = 1 

Query: 434 LAERLKKVMPSIIAPSQSGFLEGRQILDPILIANKAVEDYRIRK-KKGW-IIKLDLEKAF 493
           + ERLK +M ++I P+Q+ F+ GR   D I+   +AV   R +K  KGW ++KLDLEKA+
Sbjct: 1   MVERLKPLMTNLIGPAQASFIPGRVSTDNIVFVQEAVHSMRRKKGVKGWMLLKLDLEKAY 60

Query: 494 DIVDWAFLE 501
           D + W +LE
Sbjct: 61  DRIRWDYLE 69

[Close]
BLAST of Cucsa.356500 vs. NCBI nr
Match: gi|657990185|ref|XP_008387315.1| (PREDICTED: uncharacterized protein LOC103449768 [Malus domestica])

HSP 1 Score: 306.6 bits (784), Expect = 8.0e-80
Identity = 200/591 (33.84%), Postives = 291/591 (49.24%), Query Frame = 1 

Query: 3    QSKQLAIKTLLKKVNPDIVMIEETKREEIDSCIIKALWSSKGIGWDLVEANGRSGGLLIV 62
            + K+L +K   +++ PDI++++ETK+  ID  ++ ++W S+   W    A G SGG+ ++
Sbjct: 1013 KQKRLTLKQQFRRLQPDIIILQETKKTSIDRRLVASVWGSRFKEWIYAPAQGSSGGIAVI 1072

Query: 63   WDESKQSVLEKP-------------NWSE--LSLLSDLCTGA------------------ 122
            W+    SV E               N  E  LS +   C                     
Sbjct: 1073 WNTKNISVTESLIGVFSVSIKIKAFNGLEWWLSGVYGPCKSRERREFWEEMAGLYGLCGP 1132

Query: 123  -WCLGGDFNITRKVQERFPVRRLTRGMRKFNKFIRESKLLEIPLSNGKFSWSREGRTVSR 182
             WC+GGDFN+ R V E+    RLT  MR FN FIRE++L ++ L N +F+WS        
Sbjct: 1133 KWCVGGDFNVVRFVNEKSNGGRLTTSMRNFNDFIRETELKDLELLNAQFTWSNFREEPVC 1192

Query: 183  SLLDRFLVTDDWDESFVDTRASQKERLASDHFPILLEFGAFEWGPSPFRFCNSWLLDKQC 242
              LDRFLV+   +E F + R     R+ SDH PI LE    +WGPSPFRF N WL   + 
Sbjct: 1193 RRLDRFLVSAGCEEIFPEVRQMALARVISDHCPIQLESNKVKWGPSPFRFENMWLQHPEF 1252

Query: 243  CHIIKNSLTSGNHQGWAGFVICSKLRNLKSSLKSWLVDS----ERARKNQEESLLQ---- 302
             +       S   +GW G+    KL+  K  ++ W  +S    E+  K  E SL +    
Sbjct: 1253 RNKFNLWWQSEQVEGWEGYKFMIKLKAXKKKVQRWSKESFGEVEKDFKEAEASLEELDRR 1312

Query: 303  ------DPVILRQHYELSIKSDLLAIYRREERDLIQKCKLNWLKLGDENTSFVHRFLAAK 362
                  D    R+  EL      LA   +EE    Q+ K+ W K GD NT F HR  + +
Sbjct: 1313 EGMEGLDVDARRKREELLFXXGDLAY--KEEVKWRQRSKVEWXKEGDGNTKFFHRVASGR 1372

Query: 363  KRHSLISEL-------------IDDQVVSAEQNTRLTSIFSLPEIKSALQLLGKN----- 422
            ++ + I  L             I+D +V         S+FS  E ++   L G N     
Sbjct: 1373 RKRNYIERLEXEVGGVIEDANEIEDHIVXF-----FKSLFSSNE-EACWGLEGINWAPIS 1432

Query: 423  --------------------------KAPGPDRYTAEFLISFWDHFKDNYRALFNEFYKN 482
                                      K+PGPD Y+ + +   WD  K N   +  EFY+ 
Sbjct: 1433 ELEANWIERPFEEAEVQRAVFDCGKDKSPGPDGYSLQMIQQCWDILKANIMKIMEEFYET 1492

Query: 483  GRLNACVKENFICLIKKKEDAVRVKDFRPISLTTSIYKLVAKVLAERLKKVMPSIIAPSQ 502
            G +NA   E FICLI KK D+++V DFRPISL T +YK++AK LA RLK+V+ S I+ +Q
Sbjct: 1493 GIINAVTNETFICLIPKKSDSMKVTDFRPISLVTGLYKIMAKTLASRLKEVLGSTISQNQ 1552

[Close]
BLAST of Cucsa.356500 vs. NCBI nr
Match: gi|595882371|ref|XP_007212580.1| (hypothetical protein PRUPE_ppa015871mg, partial [Prunus persica])

HSP 1 Score: 287.0 bits (733), Expect = 6.5e-74
Identity = 167/447 (37.36%), Postives = 246/447 (55.03%), Query Frame = 1 

Query: 76  WSELSLLSDLCTGAWCLGGDFNITRKVQERFPVRRLTRGMRKFNKFIRESKLLEIPLSNG 135
           W EL+ L   C   WCLGGDFN+ R   E+    R+T+ MR FN FI+E+ L +  L N 
Sbjct: 443 WEELADLYGYCGDMWCLGGDFNVVRFSAEKSNEGRVTKSMRDFNDFIQETNLRDPILLNA 502

Query: 136 KFSWSREGRTVSRSLLDRFLVTDDWDESFVDTRASQKERLASDHFPILLEFGAFEWGPSP 195
            F+WS          LDRFLV+  W+E F   R     R+ SDH PI L+    +WGPSP
Sbjct: 503 SFTWSNLRENAVCRRLDRFLVSGSWEEHFPHYRHKALPRITSDHCPIELDTSRVKWGPSP 562

Query: 196 FRFCNSWLLDKQCCHIIKNSLTSGNHQGWAGFVICSKLRNLKSSLKSW----LVDSERAR 255
           FRF N WL        IK         GW G+   ++L+ LKS LK W      D ER  
Sbjct: 563 FRFENMWLNHPDFKRKIKLWWGEDQIPGWEGYKFMTRLKMLKSKLKVWSKEEFGDVERDL 622

Query: 256 KNQEESLLQ----------DPVILRQHYELSIKSDLLAIYRREERDLIQKCKLNWLKLGD 315
           +  E  LL           D ++  +   L +K   LA  ++EE    Q+ K+ W + GD
Sbjct: 623 REAEARLLVLDQREGTEGLDHLLRSERDNLLLKIGDLA--QKEEVKWRQRGKVKWARDGD 682

Query: 316 ENTSFVHRFLAAKKRHSLISEL-IDDQ-VVSAEQNTR------LTSIFSLPEIKSALQLL 375
            NT F HR     ++ + I +L ++D  V+  + N           ++S  + K+     
Sbjct: 683 GNTKFFHRVANGARKRNYIEKLEVEDLGVIEVDANIEREVIRFFKGLYSSNKNKAVFDC- 742

Query: 376 GKNKAPGPDRYTAEFLISFWDHFKDNYRALFNEFYKNGRLNACVKENFICLIKKKEDAVR 435
           GK+K+PGPD ++  F  S W+  K +   +  +F+++G +N    E FICLI KK ++V+
Sbjct: 743 GKDKSPGPDGFSMSFFQSCWEVVKGDLMKVMQDFFQSGIVNGVTNETFICLIPKKANSVK 802

Query: 436 VKDFRPISLTTSIYKLVAKVLAERLKKVMPSIIAPSQSGFLEGRQILDPILIANKAVEDY 495
           V D+RPISL TS+YK+++KVLA  L++V+ + I+ SQ  F++ RQILD +L+AN+ VE+ 
Sbjct: 803 VTDYRPISLVTSLYKVISKVLASSLREVLGNTISQSQGAFVQKRQILDAVLVANEVVEEV 862

Query: 496 RIRKKKGWIIKLDLEKAFDIVDWAFLE 501
           R +K+KG + K+D EKA+D V+W F++
Sbjct: 863 RKQKRKGLVFKIDFEKAYDHVEWNFVD 886

[Close]
BLAST of Cucsa.356500 vs. NCBI nr
Match: gi|595882371|ref|XP_007212580.1| (hypothetical protein PRUPE_ppa015871mg, partial [Prunus persica])

HSP 1 Score: 58.2 bits (139), Expect = 4.9e-05
Identity = 24/68 (35.29%), Postives = 44/68 (64.71%), Query Frame = 1 

Query: 5   KQLAIKTLLKKVNPDIVMIEETKREEIDSCIIKALWSSKGIGWDLVEANGRSGGLLIVWD 64
           K+L +K  L+++ PDIV++ ETK+E +D  ++  +W S+   W    + GRSGG+ ++W+
Sbjct: 338 KRLLVKEQLRRLKPDIVILLETKKEIVDRQLVAGVWGSRFKEWVFSPSLGRSGGIAVLWN 397

Query: 65  ESKQSVLE 73
               SV++
Sbjct: 398 SQSVSVID 405


HSP 2 Score: 282.0 bits (720), Expect = 2.1e-72
Identity = 191/583 (32.76%), Postives = 283/583 (48.54%), Query Frame = 1 

Query: 5   KQLAIKTLLKKVNPDIVMIEETKREEI-----------------------DSCIIKALWS 64
           K+L +K  L+++ PDIV++ ETK+E +                        S  I  LW+
Sbjct: 2   KRLLVKEQLRRLKPDIVILLETKKETVDRQLVAGVWGSRFKEWVFSPSLGRSGGIAVLWN 61

Query: 65  SKGIGW------------DLVEANGRSGGLLIVWDESKQSVLEKPNWSELSLLSDLCTGA 124
           S+ +               +VE  G    L  ++   +Q       W EL+ L   C   
Sbjct: 62  SQSVSVIDSMVGEFSVSIRIVENIGTDWWLSGIYGPCRQRE-RNSFWEELADLYGFCGDK 121

Query: 125 WCLGGDFNITRKVQERFPVRRLTRGMRKFNKFIRESKLLEIPLSNGKFSWSREGRTVSRS 184
           WCLGGDFN+ R   E+    R+T+ MR FN FI+E+ L +  L N  F+WS         
Sbjct: 122 WCLGGDFNVVRFSAEKSNEGRVTKSMRDFNDFIQETNLRDPNLLNASFTWSNLRENAVCR 181

Query: 185 LLDRFLVTDDWDESFVDTRASQKERLASDHFPILLEFGAFEWGPSPFRFCNSWLLDKQCC 244
            LDRFLV+  W++ F   R     R+ SDH PI L+    +WGPSPFRF N WL      
Sbjct: 182 RLDRFLVSGSWEDHFPHYRHKALPRITSDHCPIELDTSRVKWGPSPFRFENMWLNHPDFM 241

Query: 245 HIIKNSLTSGNHQGWAGFVICSKLRNLKSSLKSW----LVDSERARKNQEESLLQ----- 304
             IK         GW G+   ++L+ LKS LK W      D ER  +  E  LL      
Sbjct: 242 RKIKLWWGEDQIPGWEGYKFMTRLKMLKSKLKVWSKEEFGDVERDLREAEARLLVLDQRE 301

Query: 305 -----DPVILRQHYELSIKSDLLAIYRREERDLIQKCKLNWLKLGDENTSF--------- 364
                D ++  +   L +K   LA  ++EE    Q+ K+ W + GD NT F         
Sbjct: 302 GTEGLDHLLRSERDNLLLKIGDLA--QKEEVKWRQRGKVKWAREGDGNTKFFHRVANGAR 361

Query: 365 -------------------------VHRFL----AAKKRHSLISELIDDQVVSAEQNTRL 424
                                    V RF     ++ K      E ++   +S  +   L
Sbjct: 362 KRNYIEKLEVEDLGVIEVDANIEREVIRFFKGLYSSNKNVGWGVEGLNWCPISQVEADWL 421

Query: 425 TSIFSLPEIKSALQLLGKNKAPGPDRYTAEFLISFWDHFKDNYRALFNEFYKNGRLNACV 484
              F L E++ A+   GK+K+PGPD ++  F  S W+  K +   +  +F+++G +N   
Sbjct: 422 ERPFDLEEVQKAVFECGKDKSPGPDGFSMSFFQSCWEVVKGDLMKVMQDFFQSGIVNGVT 481

Query: 485 KENFICLIKKKEDAVRVKDFRPISLTTSIYKLVAKVLAERLKKVMPSIIAPSQSGFLEGR 501
            E FICLI KK ++V+V D RPISL TS+YK+++KVLA RL++V+ + I+ SQ  F++ R
Sbjct: 482 NETFICLIPKKANSVKVTDNRPISLVTSLYKVISKVLASRLREVLGNTISQSQGAFVQKR 541

[Close]
BLAST of Cucsa.356500 vs. NCBI nr
Match: gi|595905097|ref|XP_007214027.1| (hypothetical protein PRUPE_ppa016677mg [Prunus persica])

HSP 1 Score: 278.9 bits (712), Expect = 1.8e-71
Identity = 189/578 (32.70%), Postives = 279/578 (48.27%), Query Frame = 1 

Query: 10  KTLLKKVNPDIVMIEETKREEI-----------------------DSCIIKALWSSKGIG 69
           K  L+++ PDIV++ ETK+E +                        S  I  LW+S+ + 
Sbjct: 289 KEQLRRLKPDIVILLETKKEIVDRQLVAGVWGSRFKEWVFSPSLGRSGGIAVLWNSQSVS 348

Query: 70  W------------DLVEANGRSGGLLIVWDESKQSVLEKPNWSELSLLSDLCTGAWCLGG 129
                         +VE  G    L  ++   +Q       W EL+ L   C   WCLGG
Sbjct: 349 VIDSMVGEFSVSIRIVENIGTDWWLSGIYGPCRQRE-RNSFWEELADLYGYCGDKWCLGG 408

Query: 130 DFNITRKVQERFPVRRLTRGMRKFNKFIRESKLLEIPLSNGKFSWSREGRTVSRSLLDRF 189
           DFN+ R   E+    R+T+ MR FN FI+E+ L +  L N  F+WS          LDRF
Sbjct: 409 DFNVVRFSAEKSNEGRVTKSMRDFNDFIQETNLRDPILLNASFTWSNLRENAVCRRLDRF 468

Query: 190 LVTDDWDESFVDTRASQKERLASDHFPILLEFGAFEWGPSPFRFCNSWLLDKQCCHIIKN 249
           LV+  W+E F   R     R+ SDH PI L+    +WGPSPFRF N WL        IK 
Sbjct: 469 LVSGSWEEHFPHYRHKALPRITSDHCPIELDSSRVKWGPSPFRFENMWLNHPDFKRKIKL 528

Query: 250 SLTSGNHQGWAGFVICSKLRNLKSSLKSW----LVDSERARKNQEESLLQ---------- 309
                   GW G+   ++L+ LKS LK W      D ER  +  E  LL           
Sbjct: 529 WWGEDQIPGWEGYKFMTRLKMLKSKLKVWSKEEFGDVERDLREAEARLLVLDQREGTEGL 588

Query: 310 DPVILRQHYELSIKSDLLAIYRREERDLIQKCKLNWLKLGDENTSF-------------- 369
           D ++  +   L +K   LA  +REE    Q+ K+ W + GD NT F              
Sbjct: 589 DHLLRSERDNLLLKIGDLA--QREEVKWRQRGKVKWAREGDGNTKFFHRVANGARKRNYI 648

Query: 370 ---------------------VHRFLAAKKRHSLIS---ELIDDQVVSAEQNTRLTSIFS 429
                                +  F     R+  +    E ++   +S  +   L   F 
Sbjct: 649 EKLEVEDLGVIEVDANIEREVIRFFKGLYSRNKNVGWGVEGLNWCPISQVEADWLERPFD 708

Query: 430 LPEIKSALQLLGKNKAPGPDRYTAEFLISFWDHFKDNYRALFNEFYKNGRLNACVKENFI 489
           L E++ A+   GK+K+PGPD ++  F  S W+  K +   +  +F+++G +N    E FI
Sbjct: 709 LEEVQKAVFDCGKDKSPGPDGFSMSFFQSCWEVVKGDLMKVMQDFFQSGIVNGVTNETFI 768

Query: 490 CLIKKKEDAVRVKDFRPISLTTSIYKLVAKVLAERLKKVMPSIIAPSQSGFLEGRQILDP 501
           CLI KK ++V+V D+RPISL TS+YK+++KVLA RL++V+ + I+ SQ  F++ RQILD 
Sbjct: 769 CLIPKKANSVKVTDYRPISLVTSLYKVISKVLASRLREVLGNTISQSQGAFVQKRQILDA 828

[Close]
BLAST of Cucsa.356500 vs. NCBI nr
Match: gi|147790936|emb|CAN77234.1| (hypothetical protein VITISV_010061 [Vitis vinifera])

HSP 1 Score: 278.1 bits (710), Expect = 3.0e-71
Identity = 170/477 (35.64%), Postives = 239/477 (50.10%), Query Frame = 1 

Query: 76   WSELSLLSDLCTGAWCLGGDFNITRKVQERFPVRRLTRGMRKFNKFIRESKLLEIPLSNG 135
            W EL  +  L +  WC+GGDFN+ R   ER    RLT  MR+F++ I E  L ++PL  G
Sbjct: 678  WEELGAIRGLWSDPWCIGGDFNVVRFPSERSREGRLTGSMRRFSEVIEELXLKDLPLHGG 737

Query: 136  KFSWSREGRTVSRSLLDRFLVTDDWDESFVDTRASQKERLASDHFPILLEFGAFEWGPSP 195
             F+WS     +SRS LDRFL+++DW+  F         R  SDHFPILL+ G    GP P
Sbjct: 738  LFTWSGGLNGLSRSRLDRFLISEDWENHFSGAXQCSLPRPVSDHFPILLDGGGTRRGPIP 797

Query: 196  FRFCNSWLLDKQCCHIIKNSLTSGNHQGWAGFVICSKLRNLKSSLKSW------------ 255
            FRF N WL ++    ++K      N+ G   F++  KL+ LK  LK W            
Sbjct: 798  FRFENMWLKEEGFKELLKGWWQGFNYSGSYSFILTEKLKALKIKLKEWNSEVFGKVGVNK 857

Query: 256  --LVDSERARKNQEE--SLLQDPVILRQHYELSIKSDLLAIYRREERDLIQKCKLNWLKL 315
               +D      NQE+  +L +  +  R+      K   L     EE    QK +  WLK 
Sbjct: 858  GLALDKVSYWDNQEQLRALNEQELEARKEAREEFKKWALM----EETSWRQKSREIWLKE 917

Query: 316  GDENTSFVHRFLAAKKRHSLIS------------------------ELIDD--------- 375
            GD+NT F H+   + +R + +                         +L+ D         
Sbjct: 918  GDKNTGFFHKMXNSNRRRNCLKKIKVNGTWLSEDHDIQRGVVRAFXDLLSDPGGWRPCXN 977

Query: 376  ----QVVSAEQNTRLTSIFSLPEIKSALQLLGKNKAPGPDRYTAEFLISFWDHFKDNYRA 435
                  +  E+  RL   FS  E+  AL  L  +KAPGPD ++  F    WD  KD    
Sbjct: 978  NIEFDSIGDEEAARLEESFSGDEVFLALSDLNGDKAPGPDGFSLAFWQFCWDFVKDEVLG 1037

Query: 436  LFNEFYKNGRLNACVKENFICLIKKKEDAVRVKDFRPISLTTSIYKLVAKVLAERLKKVM 495
             F +FY+ G+    +   F+ LI KK  A  + DFRPISL   +YKL+AKVLA RLKKV+
Sbjct: 1038 FFKDFYERGKFVRSLNSTFLVLIPKKCGAEDLXDFRPISLVGGLYKLLAKVLANRLKKVV 1097

Query: 496  PSIIAPSQSGFLEGRQILDPILIANKAVEDYRIRKKKGWIIKLDLEKAFDIVDWAFL 500
              +++ +Q+ F+EGRQILD  L+AN+ ++    RK+ G + KLDLEKA+D ++W FL
Sbjct: 1098 GKVVSSTQNAFVEGRQILDAALVANEVIDSVLKRKESGVLCKLDLEKAYDQINWDFL 1150

The following BLAST results are available for this feature:
BLAST of Cucsa.356500 vs. Swiss-Prot
Match NameE-valueIdentityDescription
YTX2_XENLA1.2e-2236.05Transposon TX1 uncharacterized 149 kDa protein OS=Xenopus laevis PE=3 SV=1[more]
LORF2_MOUSE1.8e-1533.15LINE-1 retrotransposable element ORF2 protein OS=Mus musculus GN=Pol PE=1 SV=2[more]
LIN1_NYCCO9.9e-1431.21LINE-1 reverse transcriptase homolog OS=Nycticebus coucang PE=3 SV=1[more]
LORF2_HUMAN4.2e-1232.08LINE-1 retrotransposable element ORF2 protein OS=Homo sapiens PE=1 SV=1[more]
BLAST of Cucsa.356500 vs. TrEMBL
Match NameE-valueIdentityDescription
M5WJ76_PRUPE4.5e-7437.36Uncharacterized protein (Fragment) OS=Prunus persica GN=PRUPE_ppa015871mg PE=4 S... [more]
M5WJ76_PRUPE3.4e-0535.29Uncharacterized protein (Fragment) OS=Prunus persica GN=PRUPE_ppa015871mg PE=4 S... [more]
A5BSG1_VITVI2.1e-7135.64Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_010061 PE=4 SV=1[more]
A5BSG1_VITVI1.9e-4030.11Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_010061 PE=4 SV=1[more]
A5AN19_VITVI3.7e+0126.44Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_031023 PE=4 SV=1[more]
BLAST of Cucsa.356500 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G43760.11.8e-2125.55 DNAse I-like superfamily protein[more]
AT4G20520.12.7e-0944.93 RNA binding;RNA-directed DNA polymerases[more]
BLAST of Cucsa.356500 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|657990185|ref|XP_008387315.1|8.0e-8033.84PREDICTED: uncharacterized protein LOC103449768 [Malus domestica][more]
gi|595882371|ref|XP_007212580.1|6.5e-7437.36hypothetical protein PRUPE_ppa015871mg, partial [Prunus persica][more]
gi|595882371|ref|XP_007212580.1|4.9e-0535.29hypothetical protein PRUPE_ppa015871mg, partial [Prunus persica][more]
gi|595905097|ref|XP_007214027.1|1.8e-7132.70hypothetical protein PRUPE_ppa016677mg [Prunus persica][more]
gi|147790936|emb|CAN77234.1|3.0e-7135.64hypothetical protein VITISV_010061 [Vitis vinifera][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000477RT_dom
IPR005135Endo/exonuclease/phosphatase
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.356500.1Cucsa.356500.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000477Reverse transcriptase domainPFAMPF00078RVT_1coord: 405..499
score: 2.0
IPR000477Reverse transcriptase domainPROFILEPS50878RT_POLcoord: 386..501
score: 8
IPR005135Endonuclease/exonuclease/phosphataseGENE3DG3DSA:3.60.10.10coord: 80..186
score: 2.2
IPR005135Endonuclease/exonuclease/phosphataseunknownSSF56219DNase I-likecoord: 8..186
score: 3.0
NoneNo IPR availablePANTHERPTHR19446REVERSE TRANSCRIPTASEScoord: 42..500
score: 6.7
NoneNo IPR availablePANTHERPTHR19446:SF368SUBFAMILY NOT NAMEDcoord: 42..500
score: 6.7
NoneNo IPR availableunknownSSF56672DNA/RNA polymerasescoord: 339..498
score: 1.93

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cucsa.356500ClCG03G010430Watermelon (Charleston Gray)cgywcgB605
Cucsa.356500ClCG03G011360Watermelon (Charleston Gray)cgywcgB607
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cucsa.356500Watermelon (97103) v2cgywmbB608
Cucsa.356500Wax gourdcgywgoB711
Cucsa.356500Cucurbita maxima (Rimu)cgycmaB0969
Cucsa.356500Cucurbita maxima (Rimu)cgycmaB0970
Cucsa.356500Cucurbita moschata (Rifu)cgycmoB0972
Cucsa.356500Cucurbita moschata (Rifu)cgycmoB0973
Cucsa.356500Wild cucumber (PI 183967)cgycpiB528
Cucsa.356500Cucumber (Chinese Long) v2cgycuB500
Cucsa.356500Melon (DHL92) v3.5.1cgymeB574
Cucsa.356500Watermelon (97103) v1cgywmB638
Cucsa.356500Cucurbita pepo (Zucchini)cgycpeB0927
Cucsa.356500Cucurbita pepo (Zucchini)cgycpeB0928
Cucsa.356500Bottle gourd (USVL1VR-Ls)cgylsiB573
Cucsa.356500Melon (DHL92) v3.6.1cgymedB575
Cucsa.356500Silver-seed gourdcarcgyB0613
Cucsa.356500Silver-seed gourdcarcgyB1085
Cucsa.356500Cucumber (Chinese Long) v3cgycucB542