Cucsa.356410 (gene) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.356410
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	Peptide chain release factor 2
Location	scaffold03577 : 1209233 .. 1216794 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTTCGTACCCCATTTTTCAAATCACTTAGGTTCACGTTATCCAATTAATTTCTGTTTTTTTTCATTGAATTGATTCTGGGTTATTCTGAATCGTCTCGTATGCTTCTTTGCTCGTTTATTTTCTTTCAATGAATTTCAGTTTCTGTAAGTTGTTGGTTGCTTCCTGCAGTTCCATTTGCAACGCACGAGAGCCCGGTGGGTGAGGCAATCGACACTGATACGCGAGAATGGGCAATGCAAGGCATGTCTAGTTCCCTTACGAACAATATTTAAATTCCTAGACTGAAACTACTGTGTTCATGATGTTTTGCCCTCATTATTGAACACCCTTTGTGCCTTTATGATTCTTTTAATGCTAACTAACTAATTCTTCGAGCGTAAAGGTTTCGGAAGTAAAACTAACAAGAAGTAGCCATATAGTTGAAAGTTCTTTTGATGTCGGAAGTTTGTAATTCGAACAGTAGTTTAGTTAAGAAAAATTTATTTCTTACCATGTGTCTAATATTTCCAGCTATGGAAGATGATAAAATCTTTATTCATATAATGTTTTTTTTTCTTCATAAAAATAACTTGATGTATTTTTACTCGTGCCCAAGTGATCAAGTAAATATTGCTGTGACATATTTGGCTTCCAATGTTGGCTTTTCAAACATTAAAATTTGGTCACTATGAGGTCGCCCATAGGCTCCTTGAATCCAACCCTCTTAATGTGTTGACCTAACAATTAGTGACTTTACTAGTTGTATGTTATTCGTCAATTGTGGTCAATGCTTATGTTAGATGATGTTCAAGTTTAATTGTTGCTCTGTGAATACTCAATTGTGTGACGAATTGCTTAAGCCTTTTTATTAGTGTATATGTGAATACTCATTATACTTTATTTTTAGTTATCAACCAGTTATTGTTATTACTGCAATTTGCACGTGTGTTCTCACCATTCCGTTCTCATGCTTCATCTTTGTATTCTTTCTTGACTTTCCTTTTGATCTTCTATGGTGAACATGGATTGATTTCAGACATGGACAAACAAATTTCTTAGATCTTCTATGGTGAACATGGATTGATTTCAGACATGGACAAACAAATTTCTTACACCAGGATACCATAACTGCAATTTGGTCGGGTGATTTCCCTAAATAATCAAATTGATGCACTCTTTGTAGGAACGATTCAGAAACACAAAGTTACCTCTTCCTATTCTGCCATTACCGTCTGTTCTGCCATTACCTTCTGGAACCATCCTTCATGCTTTCTGCCATTACCTTTTGGAACCATCCTTCATGCTTTCAAAGGATATCACCATCATTCTTTCTTCTGTTATTGATGCCCATCCCTTTAATAGAAGAAAAGACATCCTTTGGAAGCACCTTTTCTATTTTAAATTTGATAGTATGAACCTCTTTTCTATTTTAAATTTGATAGTATGAGGTTCCATCTTAAAACCAATTGGCAATGAGAGAAGTAACTCATCTATATCTTATATGGAGTATGAGTCTCCTTGGTTTTTCCAATGTGAGACTCTCAACTTCTCCAACATGCTCCCTCAAGATGGTGCCTCTTTGGGAGCTCTGATACTATGATAAATTTGATAGTATGAGGTTTCATACCAAAACCAATTGACAACAAGAGGTGTAACCCATCTATCTTATATGGAGTATAAGTCTTCTTGGTTTTTCTAATGTGGGACTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGGTTTCATCTCAAAACTAATTTGCAATGAAAGGAATAAACCATCTATCTTACATGGAGTATGAGTCTCTTTGGTTTTTCCAATGTGGGACTCTCAATTTCTCCAACATCCCATGCTTTCCTTTGGCGTATTTAGCTTGAGCAAAGTGACCATATTTTCAACAACAAATTGAATGACTTTAATAATTTTGTTGATCAGGTTATTAATATCACAATTTCCTGGTGTAAACTGTCCGAGCAATTTACATCTTACAGCTTTGTTTTCCTTACGACCAATTGGAAATCCTTCCTCCTGTAATTCCTTTAGCCTGGGGCCTTCTCTCCCCTTCCTTTTGTAGTTTTCATTCCATCAATGAAATTGTTCCTTATAAAAGAAAAGAAAAAAGAAACTCATTTAAATCCCTTCCATATGCATCATTCATATGGGCCAACTATGGTTCTTGTCACACATCCACTGATAGGATCAAGCATTTTTGCATCTTGGAGCATAGCACTCTTAATTGCTTTATGTGGAAAGAATAAAGAAAGTTTCTTTGATGGAACAATTACAAAATCTACTCTTGTGAGTAAACTCCATAAATCAATTTATGGCCTCAAGCAAACTTCATGACTATGGTTTTCCAAGTTTTGTTCAGTTTTAATTGCTCATGGCTTTGTTCATTTGAAAGCAAGCCCTTCTCTCTTCATCAAGGGTGCTGGTACTTCATTTGTTACTCTCCTTTTGTACGTTGGTGACATTTACAAATATCTAAACTTCATGTTTGTTTCAGTATAAGTTGAGTCAGTATATTTCAAAGCCACATTTTGATAGTCTCGATGCTCATTATGTGTTGAGATATTTGGAAGGGAACGTTAGACAAGGGATCTTATTTTGATCTACTAACTCCTTCAAATTTGAAGGTTTTGTCGATTCTGGCTAAGGTTCATGCCTTGACACTTGACACCGTGTGATGCATTTTTGCATCTTTTTGGGGGATTCTCTTGTTTCTTGAAACTTGAAGAAACAAGCTACCATATCTTGCTCCTCAGTCGAAGTTGAATATTGAGCAGTGACCATGTTTTTTAGTGGAATCACTTAGGTTTATCATCTAATTTGTGATTTGCAATTTCAGTTCCCTCCTATAGTAGTCTTTTGTGACAATCAAGTGACTATAGCCATAGCTTTGAATCCATCTTTTCACAAGCATACAAAACATATTGAGATTGGCTCTCATTTTGTTCGAGAGAAAGTTGATTGAGGAACTTTAAAGAAGTTACCTGTTTGATCAAAATCTCACTCTCAATTAGCTAGCATGTTTACCAAGGCCTTGGACTCCTTTGTTCTTCAACAGTTCTTAGCCAAGATGGGAGCTTTCAATTTACATTTTTTTCGTGGTGGGGTACTCCTGTAGATGTGGATACTTGTTATAATTTTTGTTACATTTCTTATTAACCCATTATGGTTATTACCTGCATTTTGCATTTGTATTCTCATTGTATTTAAGAGAGGCTATTTTTAAGTATTGAAATATGATTATTTTCCATTTTACCTCTCAATTACTGTGATACTTTTATTGTTTTTATTCTGGTAAGCCGACAACTACTAGATAATATTGCGACTACTTTTAAGTCACAACAGGGTGTTTGTTGCCCCAGACTTTGATTTTACACATCAATTGTTTACATGGATACATCTAATTCCCAATTTTTCCAGCTTAGAAATGATATAAATTTTGTTCTATCTTGTATTGTCGACCTTTTTATGATGTTATGATCATTTCATGCTTTGAACCTTAGTTTGGTTGTATTTTGTATTGGGATATCATATGAGAGCATAGAGGGCATGTTAACCTTGTTTAGACATCTGGGTGCCCCCACCGACTTTTCATTCTTTAATGGCTCCTTATATCTAGAGCATTAGTCTCTTTTCATTATACCAATGAAAAATTCTCTTTCTGTTTTTTTTAAAGAACGATGGAAATGACATAATCTTTGCTTGTTGCTGTCAGATTTTTATACATTACGGAAAGAAGTGGAAACAACCTCAGAGCGTGTGGAAGAGATAAGGAACTCTGCTGGTTTGTTACAGTTGGACCAAGAACTTGCGGACTTGGAGTCTAAGGCTGCTGACAACTCTTTTTGGGATGACAGATCTAAGGCTCAAAAAGTTCTCATGGCCATGACTGATGTAAAAGATAAAATAAAGATGCTTACTGATTTCAAAACACAGGTACTTATATAGCATGCAATTTTATACCTGGACACTATTCTTTTTGAGAACCAGAAACACATGAGAGTCCACTAAAGATAGAAAACAAGCACATATATAGGCAGACAAACACACACTGACACACGTACATGTTAATTGTGCGGGCGGTATGGTTAGGAGGCCATGACCCCCCAAATTTTCTCTTTGAAATATACATGTACACGAGCACACACACACATCTTGGTTGAAATATACACACAAGCATGCACGCATGCACCACAAGAGCGTACGTGTGAATGGTAGGGGTGGGGGGGTGGTAGGATGCCATGGCCCTTTTTATTTTCTTTGAAATATATGTGCGTGTGGGCATAAATATATGCATTTCGTGTGCTTGAAAATTTTGTTTCATTTTTAATGCACTTTAACATAATTTATTCAGATCCTTGAATGTAAGGTGTGTTGCTCAAATTGGTTTAGGCATAAATCTCTTCCTTCAAGAGGGCCCCGGCCTATTCAAATCCTGTTTGAAGATTTATAAAAATATCTCCTACTTGTTTTCCAATTTATAATATCATTTAGACACATCTTTTGTTTTATGTCTTTAGAAGAAAAATGGCAATCAAGTTTAGTATCGTTAGCTTGCAGTCATAATGCAAGTGTTTGTTGAAGGGCTCTCTAAAAATTACCCAAGCATGGCTATGGCTTCCTATACATTTGTTGGAATTGCAATATTTGGTTTCTTGTTTTTTACTCTTTTCATTTTTATGATCCAATGATAAGAGGGACTCTACTTGGAAAAACATTCTCTATTAAAATGCAGAGATAAAATCGCTTTGGAAATAATTTGAAGAAAATTAGACTAAAACTGCCTTGACTGCAACCAAACTAAGCCTTAACATTTCAATCAATAGCCTAATCAAGGATTTGAAAAGTGAATGAAATCATCTCCTCAGGTGTGGGTTGTGTAATGATGATTCAGGTTGAAGAAGCAGAAACAATCGTTAAACTGACTGAAGAGATGGACTCTGTAGATGTTGGGCTTCTTGAAGAGGCAACTAAAATCATTAAAGACCTAAACAAGGCACTGGATCAATTTGAATTATCTGAACTTCTTTCTGGTCCTTATGACAAAGAAGGAGCTGTCATCTCTATCTCTGCTGGAGCTGGGGGCACTGATGCACAGGTCAATAGTTAAGGGCTTATCTTGGAAATTGTTTTCAAAGTATTTCTTTGGTCTTGTTTTCTAAACTACTTCTAGGAATTGCGGTTAGGTGTTGAATTTTGAACTCCAACTTTTTCCCAAATTTATTGCTTCTCCAATATTGAAAATTTGATAATCTCCAGTGCAGGATTGGGCTGACATGCTCTTGAGGATGTACGTGAGATGGGGAGAGAAGCAAAGATATAAAACAAGAGTAGTTGAGAAATCTGCTGGAGAGGAAGCTGGAATAAAATCAGCTACAATTGAAATTGAAGGCCGTTATGCTTATGGATATATATCTGGGGAGAAAGGAACGCACAGGATAGTGCGACAGTCTCCTTTTAACTCTAAAGGACTTCGACAGGTAATTTTTACACTAACCCCATCTTTGGTAGTGCTTGGAAATATTAAGAAAAGGTAGACACTTGAAGGCACCTAATGAATTCATGTTTTAAAATTTTCATGAACTTCCGTTAAAATGCAAAATGATTTATTGACCTCTAATGAATGATGTGAAACTAAGTGACCACTTGCTCATCTTTTCTCAAGATAGATTGTTTGATGATTGAACCTACCTGTTTTAATACAAGTTATGTGCAGACAAGCTTTTCTGGTGTGGAAGTCATGCCTCTCCTTCCCGACGAGTCCATGAATGTTGAACTGCCAGAAGAGGACCTGGAAATAAGCTTTTCAAGGGCGGGGGGAAAAGGAGGTCAAAATGTTAACAAGGTTGAAACGGCTGTCCGCATCACTCATATCCCTACAGGAGTCACGGTTCGATGCACAGGTTTCATATTCTAATTTAAAAGCACTTATAGCCACCATATAAACTTGATGTATATTCTGTTAGTAAGACTACGGTTAACAGGACAGCATCCCCAACTTTCATAAAAAATTGTTCAAAGTGATCCTCACTTAGAGCAGAATCATTTCACTTTTGTGATAAAACCAATTGATTGACATGTAAAGTGTGGTTCGGGCGTTGATTTGGGTTATAAGGTTTGTAGTTAATATATTCGAGTTAGGAAGTTTGTGTCTAGGGTGCGTAGTTGTGAGTATGGAGTTTTTCGATAAATATACAAAAGAGAGATAAAGGTAGACAAAGTTCTTCGATAAATGTACAAACTTCAATATGGAACACTATTAGAGTTGGTAGAGTTGAGTTATTTAATATAGACTTATGAAGTGGGTGGGTCAAACAACCAGTCATGTTATTGTGTTAAGTATCACTTTTCTTTGAGAAAACATTTAAGCAAAATAGTTCTTGGAAATGTTTGACGTTTTGAGTGTTGTCCTTCAATTCTTCTTGTTTGAAACCTGGAACCATTCATACTGAATGAAGCAAACCTTTCAGACTGTGACATGTTTTGATAGATTCGAATATTTCTTCGCAAAGTCACTCGTATGTTTCTTTACAACAAGTTATAAAAAATCTCTGAATTAACGGCATTTCATCTTCTGAACTATAATGCAGAGGAGAGATCGCAGCTGGCAAACAAGATCAAGGCATTAAGCCGGTTGAAAGCCAAGTTGCTAGTCATAGCTGAAGAGCAACGTGCATCCGAAATCAAGCAAATACGAGGAGATGCCGTAAAGGCCGAGTGGGGGCAACAAATAAGGAATTATGTGTTTCATCCATACAAGCTAGTGAAAGATGTTAGGACAGGATATGAGACATCAGACATTGTCTCTGTAATGGATGGCGAATTGGAGCCCTTCATCAAAGCTTACCTAAAATACAAATATAGCATTGCATTATCGACAGGAGATTAATTAAAATTTGGCATTAACTAATCTTAGTCAGCCTTGATTGTTGGTGTATTCCGCATATTGTTCTTACCTTACTATATTCAAAATAACAAGGGCACGAGGCTAAG

mRNA sequence

ATTTCGTACCCCATTTTTCAAATCACTTAGGTTCACGTTATCCAATTAATTTCTGTTTTTTTTCATTGAATTGATTCTGGGTTATTCTGAATCGTCTCGTATGCTTCTTTGCTCGTTTATTTTCTTTCAATGAATTTCAGTTTCTGTAAGTTGTTGGTTGCTTCCTGCAGTTCCATTTGCAACGCACGAGAGCCCGGTGGGTGAGGCAATCGACACTGATACGCGAGAATGGGCAATGCAAGATTTTTATACATTACGGAAAGAAGTGGAAACAACCTCAGAGCGTGTGGAAGAGATAAGGAACTCTGCTGGTTTGTTACAGTTGGACCAAGAACTTGCGGACTTGGAGTCTAAGGCTGCTGACAACTCTTTTTGGGATGACAGATCTAAGGCTCAAAAAGTTCTCATGGCCATGACTGATGTAAAAGATAAAATAAAGATGCTTACTGATTTCAAAACACAGGTTGAAGAAGCAGAAACAATCGTTAAACTGACTGAAGAGATGGACTCTGTAGATGTTGGGCTTCTTGAAGAGGCAACTAAAATCATTAAAGACCTAAACAAGGCACTGGATCAATTTGAATTATCTGAACTTCTTTCTGGTCCTTATGACAAAGAAGGAGCTGTCATCTCTATCTCTGCTGGAGCTGGGGGCACTGATGCACAGTGCAGGATTGGGCTGACATGCTCTTGAGGATGTACGTGAGATGGGGAGAGAAGCAAAGATATAAAACAAGAGTAGTTGAGAAATCTGCTGGAGAGGAAGCTGGAATAAAATCAGCTACAATTGAAATTGAAGGCCGTTATGCTTATGGATATATATCTGGGGAGAAAGGAACGCACAGGATAGTGCGACAGTCTCCTTTTAACTCTAAAGGACTTCGACAGACAAGCTTTTCTGGTGTGGAAGTCATGCCTCTCCTTCCCGACGAGTCCATGAATGTTGAACTGCCAGAAGAGGACCTGGAAATAAGCTTTTCAAGGGCGGGGGGAAAAGGAGGTCAAAATGTTAACAAGGTTGAAACGGCTGTCCGCATCACTCATATCCCTACAGGAGTCACGGTTCGATGCACAGAGGAGAGATCGCAGCTGGCAAACAAGATCAAGGCATTAAGCCGGTTGAAAGCCAAGTTGCTAGTCATAGCTGAAGAGCAACGTGCATCCGAAATCAAGCAAATACGAGGAGATGCCGTAAAGGCCGAGTGGGGGCAACAAATAAGGAATTATGTGTTTCATCCATACAAGCTAGTGAAAGATGTTAGGACAGGATATGAGACATCAGACATTGTCTCTGTAATGGATGGCGAATTGGAGCCCTTCATCAAAGCTTACCTAAAATACAAATATAGCATTGCATTATCGACAGGAGATTAATTAAAATTTGGCATTAACTAATCTTAGTCAGCCTTGATTGTTGGTGTATTCCGCATATTGTTCTTACCTTACTATATTCAAAATAACAAGGGCACGAGGCTAAG

Coding sequence (CDS)

ATGCTCTTGAGGATGTACGTGAGATGGGGAGAGAAGCAAAGATATAAAACAAGAGTAGTTGAGAAATCTGCTGGAGAGGAAGCTGGAATAAAATCAGCTACAATTGAAATTGAAGGCCGTTATGCTTATGGATATATATCTGGGGAGAAAGGAACGCACAGGATAGTGCGACAGTCTCCTTTTAACTCTAAAGGACTTCGACAGACAAGCTTTTCTGGTGTGGAAGTCATGCCTCTCCTTCCCGACGAGTCCATGAATGTTGAACTGCCAGAAGAGGACCTGGAAATAAGCTTTTCAAGGGCGGGGGGAAAAGGAGGTCAAAATGTTAACAAGGTTGAAACGGCTGTCCGCATCACTCATATCCCTACAGGAGTCACGGTTCGATGCACAGAGGAGAGATCGCAGCTGGCAAACAAGATCAAGGCATTAAGCCGGTTGAAAGCCAAGTTGCTAGTCATAGCTGAAGAGCAACGTGCATCCGAAATCAAGCAAATACGAGGAGATGCCGTAAAGGCCGAGTGGGGGCAACAAATAAGGAATTATGTGTTTCATCCATACAAGCTAGTGAAAGATGTTAGGACAGGATATGAGACATCAGACATTGTCTCTGTAATGGATGGCGAATTGGAGCCCTTCATCAAAGCTTACCTAAAATACAAATATAGCATTGCATTATCGACAGGAGATTAA

Protein sequence

MLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDESMNVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGYETSDIVSVMDGELEPFIKAYLKYKYSIALSTGD*

Homology

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BLAST of Cucsa.356410 vs. Swiss-Prot
Match: PRFB1_ARATH (Peptide chain release factor PrfB1, chloroplastic OS=Arabidopsis thaliana GN=PRFB1 PE=2 SV=1)

HSP 1 Score: 405.6 bits (1041), Expect = 3.6e-112
Identity = 197/227 (86.78%), Postives = 220/227 (96.92%), Query Frame = 1


Query: 1   MLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSP 60
           MLLRMY+RWGEKQRYKT+VVE S GEEAGIKSAT+EIEGRYAYGYISGEKGTHRIVRQSP
Sbjct: 226 MLLRMYMRWGEKQRYKTKVVEMSNGEEAGIKSATLEIEGRYAYGYISGEKGTHRIVRQSP 285

Query: 61  FNSKGLRQTSFSGVEVMPLLPDESMNVELPEEDLEISFSRAGGKGGQNVNKVETAVRITH 120
           FNSKGLRQTSFSGVEVMPLLP+E++ +E+PEEDL+ISF+RAGGKGGQNVNKVETAVRITH
Sbjct: 286 FNSKGLRQTSFSGVEVMPLLPEEAVGIEIPEEDLDISFTRAGGKGGQNVNKVETAVRITH 345

Query: 121 IPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRN 180
           IPTGV VRCTEERSQLANK +AL RLKAKL+VIAEEQRA+EIK+IRGDAVKAEWGQQIRN
Sbjct: 346 IPTGVAVRCTEERSQLANKTRALIRLKAKLMVIAEEQRATEIKEIRGDAVKAEWGQQIRN 405

Query: 181 YVFHPYKLVKDVRTGYETSDIVSVMDGELEPFIKAYLKYKYSIALST 228
           YVFHPYKLVKDVRTG+ETSDI SVMDG+L+PFIKAYLK+KY++A+++
Sbjct: 406 YVFHPYKLVKDVRTGHETSDITSVMDGDLDPFIKAYLKHKYTLAMAS 452

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BLAST of Cucsa.356410 vs. Swiss-Prot
Match: RF2_CYAP8 (Peptide chain release factor 2 OS=Cyanothece sp. (strain PCC 8801) GN=prfB PE=3 SV=1)

HSP 1 Score: 321.2 bits (822), Expect = 9.0e-87
Identity = 152/218 (69.72%), Postives = 186/218 (85.32%), Query Frame = 1


Query: 1   MLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSP 60
           MLLRMY RWGE+Q YK  + E S G+EAGIKSAT+EIEGRYAYGY+ GEKGTHR+VR SP
Sbjct: 146 MLLRMYTRWGEQQGYKVHLTEISEGDEAGIKSATLEIEGRYAYGYLKGEKGTHRLVRISP 205

Query: 61  FNSKGLRQTSFSGVEVMPLLPDESMNVELPEEDLEISFSRAGGKGGQNVNKVETAVRITH 120
           FN+ G RQTSF+G+EVMP L +E + VE+PE+DLEI+ +R+GGKGGQNVNKVETAVR+ H
Sbjct: 206 FNANGKRQTSFAGIEVMPALEEEDLKVEIPEKDLEITTTRSGGKGGQNVNKVETAVRVVH 265

Query: 121 IPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRN 180
           +PTG+ VRCT+ERSQL NK KAL+ LKAKLL+IA+EQRA  I +IRGD V+A WG QIRN
Sbjct: 266 LPTGIAVRCTQERSQLQNKEKALALLKAKLLIIAQEQRAQAIAEIRGDMVEAAWGNQIRN 325

Query: 181 YVFHPYKLVKDVRTGYETSDIVSVMDGELEPFIKAYLK 219
           YVFHPY++VKDVRT  ET+D+  VMDG+L+ FI++YL+
Sbjct: 326 YVFHPYQMVKDVRTNTETTDVNGVMDGKLDLFIESYLR 363

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BLAST of Cucsa.356410 vs. Swiss-Prot
Match: RF2_SYNY3 (Peptide chain release factor 2 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) GN=prfB PE=3 SV=2)

HSP 1 Score: 318.2 bits (814), Expect = 7.6e-86
Identity = 152/219 (69.41%), Postives = 185/219 (84.47%), Query Frame = 1


Query: 1   MLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSP 60
           MLLRMY RW EKQ YK  + E S G+EAG+KS T+EIEGRYAYGY+  EKGTHR+VR SP
Sbjct: 148 MLLRMYTRWSEKQGYKVHLAEISEGDEAGLKSVTLEIEGRYAYGYLKSEKGTHRLVRISP 207

Query: 61  FNSKGLRQTSFSGVEVMPLLPDESMNVELPEEDLEISFSRAGGKGGQNVNKVETAVRITH 120
           FN+ G RQTSF+GVEVMPLL +E++++++P++DL+IS SRAGGKGGQNVNKVETAVRI H
Sbjct: 208 FNANGKRQTSFAGVEVMPLLGEEAISLDIPDKDLDISTSRAGGKGGQNVNKVETAVRIVH 267

Query: 121 IPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRN 180
           +PTG+ VRCT+ERSQL NK KAL+ LKAKLL++ EEQRA  I +IRGD V+A WG QIRN
Sbjct: 268 LPTGLAVRCTQERSQLQNKEKALAILKAKLLIVLEEQRAQAIAEIRGDMVEAAWGTQIRN 327

Query: 181 YVFHPYKLVKDVRTGYETSDIVSVMDGELEPFIKAYLKY 220
           YVFHPY+LVKD+RT  ET+D+  VMDGEL  FI+AYL++
Sbjct: 328 YVFHPYQLVKDLRTNVETTDVGGVMDGELSDFIEAYLRH 366

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BLAST of Cucsa.356410 vs. Swiss-Prot
Match: RF2_SYNPX (Peptide chain release factor 2 OS=Synechococcus sp. (strain WH8102) GN=prfB PE=3 SV=1)

HSP 1 Score: 302.0 bits (772), Expect = 5.6e-81
Identity = 148/218 (67.89%), Postives = 179/218 (82.11%), Query Frame = 1


Query: 1   MLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSP 60
           MLLRMY RW E    K  V E S GEEAGIKS TIE+EGRYAYGY+  EKGTHR+VR SP
Sbjct: 146 MLLRMYTRWAEDHGMKVTVDELSEGEEAGIKSCTIEVEGRYAYGYLRNEKGTHRLVRISP 205

Query: 61  FNSKGLRQTSFSGVEVMPLLPDESMNVELPEEDLEISFSRAGGKGGQNVNKVETAVRITH 120
           FN+   RQTSF+G+EVMP + DE +++++PE+DLE++ SR+GG GGQNVNKVETAVRI H
Sbjct: 206 FNANDKRQTSFAGIEVMPKI-DEEVDIDIPEKDLEVTTSRSGGAGGQNVNKVETAVRILH 265

Query: 121 IPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRN 180
           IPTG+ VRCT+ERSQL NK KA++ LKAKLLVIA+EQRA+EI  IRGD V+A WG QIRN
Sbjct: 266 IPTGLAVRCTQERSQLQNKEKAMALLKAKLLVIAQEQRAAEIADIRGDIVEAAWGNQIRN 325

Query: 181 YVFHPYKLVKDVRTGYETSDIVSVMDGELEPFIKAYLK 219
           YVFHPY++VKD+RT  ET+D+ +VMDG L+PFI A L+
Sbjct: 326 YVFHPYQMVKDLRTSEETNDVQAVMDGALDPFIDASLR 362

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BLAST of Cucsa.356410 vs. Swiss-Prot
Match: RF2_SULSY (Peptide chain release factor 2 OS=Sulfurihydrogenibium sp. (strain YO3AOP1) GN=prfB PE=3 SV=1)

HSP 1 Score: 248.4 bits (633), Expect = 7.4e-65
Identity = 121/220 (55.00%), Postives = 162/220 (73.64%), Query Frame = 1


Query: 1   MLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSP 60
           MLLRMY RW EK+ Y+  +V+    + AG+KSAT+ I+G YAYGY+ GE+G HR+VR SP
Sbjct: 146 MLLRMYTRWAEKRGYQVELVDFQPDDVAGVKSATLIIKGPYAYGYLKGEQGVHRLVRISP 205

Query: 61  FNSKGLRQTSFSGVEVMPLLPDESMNVELPEEDLEISFSRAGGKGGQNVNKVETAVRITH 120
           F++   R TSFS V V+P + DE + VE+ EEDL I   RA G GGQ+VNK ++AVRITH
Sbjct: 206 FDANKRRHTSFSAVSVIPEI-DEDIKVEINEEDLRIDTYRASGAGGQHVNKTDSAVRITH 265

Query: 121 IPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRN 180
           IPTG+ V C  ERSQL NK+KA + LKAKL  +  E+R  + K++ G+     WG QIR+
Sbjct: 266 IPTGIVVACQSERSQLQNKLKATNMLKAKLYQLELEKRKEKQKELEGEKKDITWGSQIRS 325

Query: 181 YVFHPYKLVKDVRTGYETSDIVSVMDGELEPFIKAYLKYK 221
           YVF PY++VKD+RTG+ET +I +VMDGE++ FI++YLK+K
Sbjct: 326 YVFQPYQMVKDLRTGFETGNIEAVMDGEIDDFIESYLKWK 364

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BLAST of Cucsa.356410 vs. TrEMBL
Match: Q6ZY50_CUCSA (Putative translation releasing factor 2 OS=Cucumis sativus GN=rf2 PE=2 SV=1)

HSP 1 Score: 451.4 bits (1160), Expect = 6.4e-124
Identity = 229/229 (100.00%), Postives = 229/229 (100.00%), Query Frame = 1


Query: 1   MLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSP 60
           MLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSP
Sbjct: 225 MLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSP 284

Query: 61  FNSKGLRQTSFSGVEVMPLLPDESMNVELPEEDLEISFSRAGGKGGQNVNKVETAVRITH 120
           FNSKGLRQTSFSGVEVMPLLPDESMNVELPEEDLEISFSRAGGKGGQNVNKVETAVRITH
Sbjct: 285 FNSKGLRQTSFSGVEVMPLLPDESMNVELPEEDLEISFSRAGGKGGQNVNKVETAVRITH 344

Query: 121 IPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRN 180
           IPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRN
Sbjct: 345 IPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRN 404

Query: 181 YVFHPYKLVKDVRTGYETSDIVSVMDGELEPFIKAYLKYKYSIALSTGD 230
           YVFHPYKLVKDVRTGYETSDIVSVMDGELEPFIKAYLKYKYSIALSTGD
Sbjct: 405 YVFHPYKLVKDVRTGYETSDIVSVMDGELEPFIKAYLKYKYSIALSTGD 453

[Close]

BLAST of Cucsa.356410 vs. TrEMBL
Match: A0A0A0LY01_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G652260 PE=4 SV=1)

HSP 1 Score: 451.4 bits (1160), Expect = 6.4e-124
Identity = 229/229 (100.00%), Postives = 229/229 (100.00%), Query Frame = 1


Query: 1   MLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSP 60
           MLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSP
Sbjct: 225 MLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSP 284

Query: 61  FNSKGLRQTSFSGVEVMPLLPDESMNVELPEEDLEISFSRAGGKGGQNVNKVETAVRITH 120
           FNSKGLRQTSFSGVEVMPLLPDESMNVELPEEDLEISFSRAGGKGGQNVNKVETAVRITH
Sbjct: 285 FNSKGLRQTSFSGVEVMPLLPDESMNVELPEEDLEISFSRAGGKGGQNVNKVETAVRITH 344

Query: 121 IPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRN 180
           IPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRN
Sbjct: 345 IPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRN 404

Query: 181 YVFHPYKLVKDVRTGYETSDIVSVMDGELEPFIKAYLKYKYSIALSTGD 230
           YVFHPYKLVKDVRTGYETSDIVSVMDGELEPFIKAYLKYKYSIALSTGD
Sbjct: 405 YVFHPYKLVKDVRTGYETSDIVSVMDGELEPFIKAYLKYKYSIALSTGD 453

[Close]

BLAST of Cucsa.356410 vs. TrEMBL
Match: W9RF34_9ROSA (Peptide chain release factor 2 OS=Morus notabilis GN=L484_005584 PE=4 SV=1)

HSP 1 Score: 429.1 bits (1102), Expect = 3.4e-117
Identity = 213/227 (93.83%), Postives = 225/227 (99.12%), Query Frame = 1


Query: 1   MLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSP 60
           MLLRMYVRWGEKQRYKTRVVEKS GEEAGIKSATIE+EGRYAYGY+SGEKGTHRIVRQSP
Sbjct: 231 MLLRMYVRWGEKQRYKTRVVEKSPGEEAGIKSATIEVEGRYAYGYLSGEKGTHRIVRQSP 290

Query: 61  FNSKGLRQTSFSGVEVMPLLPDESMNVELPEEDLEISFSRAGGKGGQNVNKVETAVRITH 120
           FNSKGLRQTSFSGVEVMPLLP+ES+N+E+P+EDL+ISFSRAGGKGGQNVNKVETAVRITH
Sbjct: 291 FNSKGLRQTSFSGVEVMPLLPEESINIEIPDEDLDISFSRAGGKGGQNVNKVETAVRITH 350

Query: 121 IPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRN 180
           IPTGVT+RCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRN
Sbjct: 351 IPTGVTLRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRN 410

Query: 181 YVFHPYKLVKDVRTGYETSDIVSVMDGELEPFIKAYLKYKYSIALST 228
           YVFHPYKLVKDVRTG ETSDIVSVMDGELEPFIKA+LKYKYS+A+ST
Sbjct: 411 YVFHPYKLVKDVRTGLETSDIVSVMDGELEPFIKAFLKYKYSMAVST 457

[Close]

BLAST of Cucsa.356410 vs. TrEMBL
Match: V4SMM1_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10012132mg PE=4 SV=1)

HSP 1 Score: 428.7 bits (1101), Expect = 4.4e-117
Identity = 213/229 (93.01%), Postives = 225/229 (98.25%), Query Frame = 1


Query: 1   MLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSP 60
           MLLRMYVRWGEKQRYKTRVVEKS GEEAGIKSA IE+EGRYAYGY+SGEKGTHRIVRQSP
Sbjct: 111 MLLRMYVRWGEKQRYKTRVVEKSLGEEAGIKSAVIEVEGRYAYGYLSGEKGTHRIVRQSP 170

Query: 61  FNSKGLRQTSFSGVEVMPLLPDESMNVELPEEDLEISFSRAGGKGGQNVNKVETAVRITH 120
           FN+KGLRQTSFSGVEVMPLLP+ESM+V++PEEDLEISFSRAGGKGGQNVNKVETAVRITH
Sbjct: 171 FNAKGLRQTSFSGVEVMPLLPEESMDVQIPEEDLEISFSRAGGKGGQNVNKVETAVRITH 230

Query: 121 IPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRN 180
           IPTGVTVRCTEERSQLANKIKALS+LKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRN
Sbjct: 231 IPTGVTVRCTEERSQLANKIKALSQLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRN 290

Query: 181 YVFHPYKLVKDVRTGYETSDIVSVMDGELEPFIKAYLKYKYSIALSTGD 230
           YVFHPYKLVKDVRTG+ETSDIVSVMDGELEPFIK+YLKYKYS++LS  D
Sbjct: 291 YVFHPYKLVKDVRTGHETSDIVSVMDGELEPFIKSYLKYKYSMSLSASD 339

[Close]

BLAST of Cucsa.356410 vs. TrEMBL
Match: D7SPM1_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_04s0023g00870 PE=4 SV=1)

HSP 1 Score: 428.7 bits (1101), Expect = 4.4e-117
Identity = 214/226 (94.69%), Postives = 223/226 (98.67%), Query Frame = 1


Query: 1   MLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSP 60
           MLLRMYVRWGEKQRYKTRVVEKS GEEAGIKSATIE+EGRYAYGY+SGEKGTHRIVRQSP
Sbjct: 223 MLLRMYVRWGEKQRYKTRVVEKSMGEEAGIKSATIELEGRYAYGYLSGEKGTHRIVRQSP 282

Query: 61  FNSKGLRQTSFSGVEVMPLLPDESMNVELPEEDLEISFSRAGGKGGQNVNKVETAVRITH 120
           FN+KGLRQTSFSGVEVMPLLP+ESM+VE+PEEDLEISFSRAGGKGGQNVNKVETAVRITH
Sbjct: 283 FNAKGLRQTSFSGVEVMPLLPEESMDVEIPEEDLEISFSRAGGKGGQNVNKVETAVRITH 342

Query: 121 IPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRN 180
           IPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRN
Sbjct: 343 IPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRN 402

Query: 181 YVFHPYKLVKDVRTGYETSDIVSVMDGELEPFIKAYLKYKYSIALS 227
           YVFHPYKLVKDVRTGYETSDI SVMDGELEPFIKAYLK+K+S+ LS
Sbjct: 403 YVFHPYKLVKDVRTGYETSDITSVMDGELEPFIKAYLKFKFSMTLS 448

[Close]

BLAST of Cucsa.356410 vs. TAIR10
Match: AT5G36170.1 (AT5G36170.1 high chlorophyll fluorescent 109)

HSP 1 Score: 405.6 bits (1041), Expect = 2.0e-113
Identity = 197/227 (86.78%), Postives = 220/227 (96.92%), Query Frame = 1


Query: 1   MLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSP 60
           MLLRMY+RWGEKQRYKT+VVE S GEEAGIKSAT+EIEGRYAYGYISGEKGTHRIVRQSP
Sbjct: 226 MLLRMYMRWGEKQRYKTKVVEMSNGEEAGIKSATLEIEGRYAYGYISGEKGTHRIVRQSP 285

Query: 61  FNSKGLRQTSFSGVEVMPLLPDESMNVELPEEDLEISFSRAGGKGGQNVNKVETAVRITH 120
           FNSKGLRQTSFSGVEVMPLLP+E++ +E+PEEDL+ISF+RAGGKGGQNVNKVETAVRITH
Sbjct: 286 FNSKGLRQTSFSGVEVMPLLPEEAVGIEIPEEDLDISFTRAGGKGGQNVNKVETAVRITH 345

Query: 121 IPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRN 180
           IPTGV VRCTEERSQLANK +AL RLKAKL+VIAEEQRA+EIK+IRGDAVKAEWGQQIRN
Sbjct: 346 IPTGVAVRCTEERSQLANKTRALIRLKAKLMVIAEEQRATEIKEIRGDAVKAEWGQQIRN 405

Query: 181 YVFHPYKLVKDVRTGYETSDIVSVMDGELEPFIKAYLKYKYSIALST 228
           YVFHPYKLVKDVRTG+ETSDI SVMDG+L+PFIKAYLK+KY++A+++
Sbjct: 406 YVFHPYKLVKDVRTGHETSDITSVMDGDLDPFIKAYLKHKYTLAMAS 452

[Close]

BLAST of Cucsa.356410 vs. TAIR10
Match: AT1G56350.1 (AT1G56350.1 Peptide chain release factor 2)

HSP 1 Score: 206.8 bits (525), Expect = 1.4e-53
Identity = 100/217 (46.08%), Postives = 144/217 (66.36%), Query Frame = 1


Query: 1   MLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSP 60
           M++ MY  W +++++   VV+++ GE AGIK ATI++ G YAYGY   E G HR+VR SP
Sbjct: 255 MVMEMYKTWAQRRKFSVTVVDEAPGEIAGIKRATIKVNGEYAYGYAKAEVGVHRLVRISP 314

Query: 61  FNSKGLRQTSFSGVEVMPLLPDESMNVELPEEDLEISFSRAGGKGGQNVNKVETAVRITH 120
           F+S   R TSF+ V V+P+L D S  VE+ + DL I   R+GG GGQ+ N  ++AVRI H
Sbjct: 315 FDSGKRRHTSFAAVAVIPILGDGSTRVEINDSDLRIERFRSGGAGGQHANTTDSAVRIVH 374

Query: 121 IPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRN 180
           IPTG+T  C  ERSQ +NK  A++ L+++L  +   ++ +   Q      +  WG QIR 
Sbjct: 375 IPTGITATCQNERSQHSNKASAMAVLQSRLDQLEMARQTAMNAQHTQSLTEISWGNQIRT 434

Query: 181 YVFHPYKLVKDVRTGYETSDIVSVMDGELEPFIKAYL 218
           YV HPY++VKD+RT YE SD  SV++G+L+ FI ++L
Sbjct: 435 YVLHPYRMVKDLRTNYEVSDPDSVLEGDLDGFILSFL 471

[Close]

BLAST of Cucsa.356410 vs. TAIR10
Match: AT3G57190.1 (AT3G57190.1 peptide chain release factor, putative)

HSP 1 Score: 147.9 bits (372), Expect = 7.7e-36
Identity = 85/224 (37.95%), Postives = 137/224 (61.16%), Query Frame = 1


Query: 2   LLRMYVRWGEKQRYKTRVVEKSA--GEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQS 61
           ++ MY++W E+     RV EK +    ++G+ SATIE E  +AYGY+ GE+G HR++  S
Sbjct: 191 VVSMYIKWAERLGQNARVAEKCSLLSNKSGVSSATIEFEFEFAYGYLLGERGVHRLIISS 250

Query: 62  PFNSKGLRQTSFSGVEVMPLLPDESMNVELPEEDLEISFSRAGGKGGQNVNKVETAVRIT 121
             N +       + V+++PL    S + E+ E DL +S+        ++    E  V I 
Sbjct: 251 TSNEE-----CSATVDIIPLFLRASPDFEVKEGDLIVSYPAK-----EDHKIAENMVCIH 310

Query: 122 HIPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIR 181
           HIP+GVT++ + ER++ AN+IKAL+RLKAKLLVIA+EQ+ S++ +I    +  E  ++ R
Sbjct: 311 HIPSGVTLQSSGERNRFANRIKALNRLKAKLLVIAKEQKVSDVNKIDSKNI-LEPREETR 370

Query: 182 NYVFHPYKLVKDVRTGYETSDIVSVMDGELEPFIKAYLKYKYSI 224
           +YV   +K+V D +TG E  D+ SV+DG + P + A++  + SI
Sbjct: 371 SYVSKGHKMVVDRKTGLEILDLKSVLDGNIGPLLGAHISMRRSI 403

[Close]

BLAST of Cucsa.356410 vs. TAIR10
Match: AT3G62910.1 (AT3G62910.1 Peptide chain release factor 1)

HSP 1 Score: 127.1 bits (318), Expect = 1.4e-29
Identity = 75/215 (34.88%), Postives = 121/215 (56.28%), Query Frame = 1


Query: 2   LLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPF 61
           L+RMY R+ E+  +K  +V  S  E  G K+  +EI+G   Y  +  E G HR+ R    
Sbjct: 187 LVRMYQRYSERSSWKFSMVSCSEAEHGGYKTCVMEIKGNRVYSKLKYESGVHRVQRVPQT 246

Query: 62  NSKGLRQTSFSGVEVMPLLPDESMNVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHI 121
            ++G   TS + V +MP    + + V +  +D+E++ +R+GG GGQNVNKVETA+ + H 
Sbjct: 247 ETQGRVHTSTATVAIMP--EADEVEVVIDPKDIELTSARSGGAGGQNVNKVETAIDLFHK 306

Query: 122 PTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAV-KAEWGQQIRN 181
           P+G+ + CTEER+Q+ NK +A   L+AKL  I   ++  +I+  R   V      ++IR 
Sbjct: 307 PSGIRIFCTEERTQIRNKARAFQLLRAKLYEIKVREQQEKIRNERKSQVGTGARSEKIRT 366

Query: 182 YVFHPYKLVKDVRTGYETSDIVSVMDGELEPFIKA 216
           Y +   + V D R     + + + +DG LE  ++A
Sbjct: 367 YNYKDSR-VTDHRLKMNFA-LTTFLDGALEDAVQA 397

[Close]

BLAST of Cucsa.356410 vs. TAIR10
Match: AT2G47020.1 (AT2G47020.1 Peptide chain release factor 1)

HSP 1 Score: 116.7 bits (291), Expect = 1.9e-26
Identity = 79/218 (36.24%), Postives = 113/218 (51.83%), Query Frame = 1


Query: 2   LLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPF 61
           + RMY R+ +K+ +K  +V+ +  +  G K A+  I G   YG +  E G HR+ R    
Sbjct: 184 IFRMYERYSQKKGWKFDIVDITESDMKGYKEASAAICGASVYGKLKFESGIHRVQRIPIT 243

Query: 62  NSKGLRQTSFSGVEVMPLLPDESMNVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHI 121
              G   TS   V ++P    + ++V+L  EDL I   R+GG GGQ+ N   +AVRI H+
Sbjct: 244 EKSGRIHTSAISVAILPQA--DEVDVQLRNEDLRIDTYRSGGSGGQHANTTNSAVRIIHL 303

Query: 122 PTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAV-KAEWGQQIRN 181
           PTG+ V   +ERSQ  N+ KAL  L A+L  I   +  S   ++R D +   +   +IR 
Sbjct: 304 PTGMMVSIQDERSQHMNRAKALKVLCARLYEIERLRIQSSRSKLRSDQIGSGDRSGRIRT 363

Query: 182 YVFHPYKLVKDVRTGYETSDIVSVMDGE-LEPFIKAYL 218
           Y F P   V D R G     I  +M GE L+ FI A L
Sbjct: 364 YNF-PQGRVTDHRVGITHHAIEDMMQGENLDMFIDALL 398

[Close]

BLAST of Cucsa.356410 vs. NCBI nr
Match: gi|793422635|ref|NP_001292716.1| (peptide chain release factor PrfB1, chloroplastic [Cucumis sativus])

HSP 1 Score: 451.4 bits (1160), Expect = 9.2e-124
Identity = 229/229 (100.00%), Postives = 229/229 (100.00%), Query Frame = 1


Query: 1   MLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSP 60
           MLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSP
Sbjct: 225 MLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSP 284

Query: 61  FNSKGLRQTSFSGVEVMPLLPDESMNVELPEEDLEISFSRAGGKGGQNVNKVETAVRITH 120
           FNSKGLRQTSFSGVEVMPLLPDESMNVELPEEDLEISFSRAGGKGGQNVNKVETAVRITH
Sbjct: 285 FNSKGLRQTSFSGVEVMPLLPDESMNVELPEEDLEISFSRAGGKGGQNVNKVETAVRITH 344

Query: 121 IPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRN 180
           IPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRN
Sbjct: 345 IPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRN 404

Query: 181 YVFHPYKLVKDVRTGYETSDIVSVMDGELEPFIKAYLKYKYSIALSTGD 230
           YVFHPYKLVKDVRTGYETSDIVSVMDGELEPFIKAYLKYKYSIALSTGD
Sbjct: 405 YVFHPYKLVKDVRTGYETSDIVSVMDGELEPFIKAYLKYKYSIALSTGD 453

[Close]

BLAST of Cucsa.356410 vs. NCBI nr
Match: gi|778664042|ref|XP_011660203.1| (PREDICTED: peptide chain release factor PrfB1, chloroplastic isoform X3 [Cucumis sativus])

HSP 1 Score: 451.4 bits (1160), Expect = 9.2e-124
Identity = 229/229 (100.00%), Postives = 229/229 (100.00%), Query Frame = 1


Query: 1   MLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSP 60
           MLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSP
Sbjct: 229 MLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSP 288

Query: 61  FNSKGLRQTSFSGVEVMPLLPDESMNVELPEEDLEISFSRAGGKGGQNVNKVETAVRITH 120
           FNSKGLRQTSFSGVEVMPLLPDESMNVELPEEDLEISFSRAGGKGGQNVNKVETAVRITH
Sbjct: 289 FNSKGLRQTSFSGVEVMPLLPDESMNVELPEEDLEISFSRAGGKGGQNVNKVETAVRITH 348

Query: 121 IPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRN 180
           IPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRN
Sbjct: 349 IPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRN 408

Query: 181 YVFHPYKLVKDVRTGYETSDIVSVMDGELEPFIKAYLKYKYSIALSTGD 230
           YVFHPYKLVKDVRTGYETSDIVSVMDGELEPFIKAYLKYKYSIALSTGD
Sbjct: 409 YVFHPYKLVKDVRTGYETSDIVSVMDGELEPFIKAYLKYKYSIALSTGD 457

[Close]

BLAST of Cucsa.356410 vs. NCBI nr
Match: gi|700211547|gb|KGN66643.1| (hypothetical protein Csa_1G652260 [Cucumis sativus])

HSP 1 Score: 451.4 bits (1160), Expect = 9.2e-124
Identity = 229/229 (100.00%), Postives = 229/229 (100.00%), Query Frame = 1


Query: 1   MLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSP 60
           MLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSP
Sbjct: 225 MLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSP 284

Query: 61  FNSKGLRQTSFSGVEVMPLLPDESMNVELPEEDLEISFSRAGGKGGQNVNKVETAVRITH 120
           FNSKGLRQTSFSGVEVMPLLPDESMNVELPEEDLEISFSRAGGKGGQNVNKVETAVRITH
Sbjct: 285 FNSKGLRQTSFSGVEVMPLLPDESMNVELPEEDLEISFSRAGGKGGQNVNKVETAVRITH 344

Query: 121 IPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRN 180
           IPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRN
Sbjct: 345 IPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRN 404

Query: 181 YVFHPYKLVKDVRTGYETSDIVSVMDGELEPFIKAYLKYKYSIALSTGD 230
           YVFHPYKLVKDVRTGYETSDIVSVMDGELEPFIKAYLKYKYSIALSTGD
Sbjct: 405 YVFHPYKLVKDVRTGYETSDIVSVMDGELEPFIKAYLKYKYSIALSTGD 453

[Close]

BLAST of Cucsa.356410 vs. NCBI nr
Match: gi|778664039|ref|XP_011660202.1| (PREDICTED: peptide chain release factor PrfB1, chloroplastic isoform X2 [Cucumis sativus])

HSP 1 Score: 451.4 bits (1160), Expect = 9.2e-124
Identity = 229/229 (100.00%), Postives = 229/229 (100.00%), Query Frame = 1


Query: 1   MLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSP 60
           MLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSP
Sbjct: 235 MLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSP 294

Query: 61  FNSKGLRQTSFSGVEVMPLLPDESMNVELPEEDLEISFSRAGGKGGQNVNKVETAVRITH 120
           FNSKGLRQTSFSGVEVMPLLPDESMNVELPEEDLEISFSRAGGKGGQNVNKVETAVRITH
Sbjct: 295 FNSKGLRQTSFSGVEVMPLLPDESMNVELPEEDLEISFSRAGGKGGQNVNKVETAVRITH 354

Query: 121 IPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRN 180
           IPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRN
Sbjct: 355 IPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRN 414

Query: 181 YVFHPYKLVKDVRTGYETSDIVSVMDGELEPFIKAYLKYKYSIALSTGD 230
           YVFHPYKLVKDVRTGYETSDIVSVMDGELEPFIKAYLKYKYSIALSTGD
Sbjct: 415 YVFHPYKLVKDVRTGYETSDIVSVMDGELEPFIKAYLKYKYSIALSTGD 463

[Close]

BLAST of Cucsa.356410 vs. NCBI nr
Match: gi|778664036|ref|XP_011660200.1| (PREDICTED: peptide chain release factor PrfB1, chloroplastic isoform X1 [Cucumis sativus])

HSP 1 Score: 451.4 bits (1160), Expect = 9.2e-124
Identity = 229/229 (100.00%), Postives = 229/229 (100.00%), Query Frame = 1


Query: 1   MLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSP 60
           MLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSP
Sbjct: 239 MLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSP 298

Query: 61  FNSKGLRQTSFSGVEVMPLLPDESMNVELPEEDLEISFSRAGGKGGQNVNKVETAVRITH 120
           FNSKGLRQTSFSGVEVMPLLPDESMNVELPEEDLEISFSRAGGKGGQNVNKVETAVRITH
Sbjct: 299 FNSKGLRQTSFSGVEVMPLLPDESMNVELPEEDLEISFSRAGGKGGQNVNKVETAVRITH 358

Query: 121 IPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRN 180
           IPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRN
Sbjct: 359 IPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRN 418

Query: 181 YVFHPYKLVKDVRTGYETSDIVSVMDGELEPFIKAYLKYKYSIALSTGD 230
           YVFHPYKLVKDVRTGYETSDIVSVMDGELEPFIKAYLKYKYSIALSTGD
Sbjct: 419 YVFHPYKLVKDVRTGYETSDIVSVMDGELEPFIKAYLKYKYSIALSTGD 467

The following BLAST results are available for this feature:

BLAST of Cucsa.356410 vs. Swiss-Prot


Match Name	E-value	Identity	Description
PRFB1_ARATH	3.6e-112	86.78	Peptide chain release factor PrfB1, chloroplastic OS=Arabidopsis thaliana GN=PRF...	[more]
RF2_CYAP8	9.0e-87	69.72	Peptide chain release factor 2 OS=Cyanothece sp. (strain PCC 8801) GN=prfB PE=3 ...	[more]
RF2_SYNY3	7.6e-86	69.41	Peptide chain release factor 2 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) G...	[more]
RF2_SYNPX	5.6e-81	67.89	Peptide chain release factor 2 OS=Synechococcus sp. (strain WH8102) GN=prfB PE=3...	[more]
RF2_SULSY	7.4e-65	55.00	Peptide chain release factor 2 OS=Sulfurihydrogenibium sp. (strain YO3AOP1) GN=p...	[more]

BLAST of Cucsa.356410 vs. TrEMBL


Match Name	E-value	Identity	Description
Q6ZY50_CUCSA	6.4e-124	100.00	Putative translation releasing factor 2 OS=Cucumis sativus GN=rf2 PE=2 SV=1	[more]
A0A0A0LY01_CUCSA	6.4e-124	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G652260 PE=4 SV=1	[more]
W9RF34_9ROSA	3.4e-117	93.83	Peptide chain release factor 2 OS=Morus notabilis GN=L484_005584 PE=4 SV=1	[more]
V4SMM1_9ROSI	4.4e-117	93.01	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10012132mg PE=4 SV=1	[more]
D7SPM1_VITVI	4.4e-117	94.69	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_04s0023g00870 PE=4 SV=...	[more]

BLAST of Cucsa.356410 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G36170.1	2.0e-113	86.78	high chlorophyll fluorescent 109	[more]
AT1G56350.1	1.4e-53	46.08	Peptide chain release factor 2	[more]
AT3G57190.1	7.7e-36	37.95	peptide chain release factor, putative	[more]
AT3G62910.1	1.4e-29	34.88	Peptide chain release factor 1	[more]
AT2G47020.1	1.9e-26	36.24	Peptide chain release factor 1	[more]

BLAST of Cucsa.356410 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|793422635\|ref\|NP_001292716.1\|	9.2e-124	100.00	peptide chain release factor PrfB1, chloroplastic [Cucumis sativus]	[more]
gi\|778664042\|ref\|XP_011660203.1\|	9.2e-124	100.00	PREDICTED: peptide chain release factor PrfB1, chloroplastic isoform X3 [Cucumis...	[more]
gi\|700211547\|gb\|KGN66643.1\|	9.2e-124	100.00	hypothetical protein Csa_1G652260 [Cucumis sativus]	[more]
gi\|778664039\|ref\|XP_011660202.1\|	9.2e-124	100.00	PREDICTED: peptide chain release factor PrfB1, chloroplastic isoform X2 [Cucumis...	[more]
gi\|778664036\|ref\|XP_011660200.1\|	9.2e-124	100.00	PREDICTED: peptide chain release factor PrfB1, chloroplastic isoform X1 [Cucumis...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR000352	Pep_chain_release_fac_I_II
IPR005139	PCRF
IPR014720	dsRBD_dom

Vocabulary: **Molecular Function**
Term	Definition
GO:0003747	translation release factor activity
GO:0016149	translation release factor activity, codon specific

Vocabulary: **Biological Process**
Term	Definition
GO:0006415	translational termination

Vocabulary: **Cellular Component**
Term	Definition
GO:0005737	cytoplasm

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030154	cell differentiation
biological_process	GO:0006449	regulation of translational termination
biological_process	GO:0006415	translational termination
biological_process	GO:0042793	transcription from plastid promoter
biological_process	GO:0009902	chloroplast relocation
biological_process	GO:0006364	rRNA processing
biological_process	GO:0010027	thylakoid membrane organization
biological_process	GO:0035304	regulation of protein dephosphorylation
biological_process	GO:0045893	positive regulation of transcription, DNA-templated
biological_process	GO:0010207	photosystem II assembly
biological_process	GO:0009965	leaf morphogenesis
biological_process	GO:0051083	'de novo' cotranslational protein folding
cellular_component	GO:0009570	chloroplast stroma
cellular_component	GO:0005840	ribosome
cellular_component	GO:0018444	translation release factor complex
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0016149	translation release factor activity, codon specific
molecular_function	GO:0003747	translation release factor activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsa.356410.1	Cucsa.356410.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000352	Peptide chain release factor class I/class II	PFAM	PF00472	RF-1	coord: 85..193 score: 1.2
IPR000352	Peptide chain release factor class I/class II	PROSITE	PS00745	RF_PROK_I	coord: 100..116 scor
IPR005139	Peptide chain release factor	PFAM	PF03462	PCRF	coord: 1..74 score: 2.1
IPR014720	Double-stranded RNA-binding domain	GENE3D	G3DSA:3.30.160.20		coord: 83..161 score: 7.7
None	No IPR available	PANTHER	PTHR11075	PEPTIDE CHAIN RELEASE FACTOR	coord: 1..228 score: 4.1E
None	No IPR available	PANTHER	PTHR11075:SF46	PROTEIN HIGH CHLOROPHYLL FLUORESCENT 109	coord: 1..228 score: 4.1E
None	No IPR available	unknown	SSF75620	Release factor	coord: 1..221 score: 3.01

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cucsa.356410	Csa1G652260	Cucumber (Chinese Long) v2	cgycuB500
Cucsa.356410	MELO3C010022	Melon (DHL92) v3.5.1	cgymeB574
Cucsa.356410	CSPI01G32010	Wild cucumber (PI 183967)	cgycpiB528
Cucsa.356410	MELO3C010022.2	Melon (DHL92) v3.6.1	cgymedB575
Cucsa.356410	CsaV3_1G044280	Cucumber (Chinese Long) v3	cgycucB542

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cucsa.356410	Cucurbita pepo (Zucchini)	cgycpeB0927
Cucsa.356410	Cucurbita pepo (Zucchini)	cgycpeB0928
Cucsa.356410	Bottle gourd (USVL1VR-Ls)	cgylsiB573
Cucsa.356410	Silver-seed gourd	carcgyB0613
Cucsa.356410	Silver-seed gourd	carcgyB1085
Cucsa.356410	Watermelon (97103) v2	cgywmbB608
Cucsa.356410	Wax gourd	cgywgoB711
Cucsa.356410	Cucurbita maxima (Rimu)	cgycmaB0969
Cucsa.356410	Cucurbita maxima (Rimu)	cgycmaB0970
Cucsa.356410	Cucurbita moschata (Rifu)	cgycmoB0972
Cucsa.356410	Cucurbita moschata (Rifu)	cgycmoB0973
Cucsa.356410	Watermelon (Charleston Gray)	cgywcgB605
Cucsa.356410	Watermelon (Charleston Gray)	cgywcgB607
Cucsa.356410	Watermelon (97103) v1	cgywmB638