Cucsa.341380 (gene) Cucumber (Gy14) v1

NameCucsa.341380
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionIAA-amino acid hydrolase ILR1, putative
Locationscaffold03356 : 3772197 .. 3776035 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCTTTTTGGACATTACATTACATTGAGCTTTTAAGCTTTCTCTTCATCGCCTCTTTTCTTCTTCCCCTGTGGCTCTTCTCATTCCTATCCCCACCCAGCAATACCCACTTCTCAATTCCTTCtCtttTTTTTTTTtCTTTTTTTtCTTTTTTACCCATTTTAACTTTTCAATTTCTTGTTTCTTCACTATATGCATTTAATCATGGCTTCCCTCAGGTGGATTTCTTGGATTTTGATCGCACATTTGTTTGTTTCAACATTTGTGCACTCAGATTCTTCCTCCCTCTCAAATAATCCCCAAGAAATTCCTAAAAAGTTCCTCCAATTTGCTAAGGAATCTGAGCTGTTTGATTGGATGGTGGGCATCCGTAGGAAAATTCACGAGAACCCAGAACTTGGTTTTGAGGAATTCGAGACCAGTAAGCTGATTAGAACGGAATTAGATAAATTGGGCATTTCTTATAAATACCCAGTTGCCAGTACTGGAGTTATCGGATTTGTTGGTTCTGGTCAGCCCCCTTTTGTGGCTATTCGAGCAGATATGGATGCTCTTCCTATGCAGGTTTGAATCGATTGGTTGACTTATGTAACTCTTTTCTGATGGTTCTAGTTGATAATTCAGTGTCGAGTGAAATCTGCCTGCCAGATGTTTGATTATATGCCAAAACATCGATGATCGTACTCTTGGTCTCTTCGTGTTCCTTTGATGTTATGATAGTGAATTGAATAGCAATTTGCCCCAGACATGATTGAATTTCTTACTGAGTGAAATTCGGTTTTTACAGGAATTGGTGGAGTGGGAGCATAAGAGTAAGGTTCCTGGGAAGATGCACGCTTGTGGTCACGATGCTCATGTTGCGATGGTCTTGGGTGCTGCAAAGATCCTTCAAAAGCATAGCGAGGAGTTGAAAGTAAGTAAACATCTCGTGGATCATTTTCTGTTCAAATGTGTAAGTTGCAAACAAAGTCCCCATGTTGGTTAGGGAGAGATCTATAGAATGTGTGAGGACAATTTTCTGTGTATGAGGCATTACAGGTGAAACCAGAAGCAAATTCAAGAAGGTTTAGGCTTAAATTGAACAATATCATACCATCGTTGAAATATGTGGACGTTTGTTGTCCTAAACAAAAGGGATGAATACTTCATCTCATGATTTATATTGAGCAATTGACAGTCTCTAGATTTCTTGGATCTTGTAGATCATTAAAAAGAGTATTTGTACCAACATCATGTGCTCATTTTTGGCATCCAGATTTTCATGTGCTAGAGTTAAGTTAGTGGACTGTGATTATTTTGGTTGGATGATAATTTGATTCTATGGAGTTTTTTTtGTTCCACTGCCTCGTCTATGGAGATCATGAATTACTTTGAATCTTCCACAGTTACTTTCCTTTGTCATCACTTGTTTGTATGATCCACAGTCGAAAATTTAAGTGACTAAAAGTGCTTGAAAATGTTATCGTATTTTTAGATGTGCACAATTTTATAGGAAGAAATTTTGAAAATTATTTGCTGGATCTTGAAGCTTGTCCAATATAGTGAATCACACTGGGTTTGCTGATTGGTTGCATTGGATCAAATTAGATGAACTTCTTGTCTTACTTTCCTTTTATTTTCTTTCTCTTCTTACGCTTATACTATCCTGGAGCGAAACTTTtGTTTAAAGTGCATTACCTTTTCACACCAAAAGATTATATTGTGCATTTGAAGCCAACTACTTCTGCAATGCGTATAAATGTTTAAGCAATCTTCCTCTTCACTCTGGTGGGGCGGCAAGAAAAAAaTGGATGTTCCTTAAACTTGTACACCTTTACACTACTCTAATCAATAGCTAATGTTGTTGCCTGTTTTCTTTGATTCTATTCGTGCAACAGGGGACAGTTGTACTAGTTTTCCAACCAGCTGAAGAAGGAGGTGGAGGTGCTATGAAAATAATAGAAGCTGGAGTGTTGGATAATGTCAATGCTATCTTTGGGTTGCATATTGTTCATAATATACCTATCGGTAAAGTGGCTGGACGCTCAGGACCCCTGTTAGCTGGAAGTGCCTTTTTTGAAGCAGTAATCAGTGGAAAAGGAGGCCATGCTGCCATTCCTCAGCATTCTATAGACCCAATTTTGGCTGCATCTAACGTTATTGTGAGCTTACAACATCTTGTTTCAAGGGAAGCTGATCCACTAGATTCACAGGTAGAGCATATTTCCTGTTTTGTTTTCATCAGTTCCAAGTAACAAGAATGTCATTACCCTTGTCTGAAGAGTTATGGCTACCTCTCTGTGTTCCCATATCCGTTATAACAGTAACATGTAAATAGTGTTAATGTATTTATTTGTTGAGCTGTTGTTGCATTACCCTTGTGATCAGTCTTCTGCTTCTGAATATATTGGCGAGGAAGGTTGCATTGACAATCAATAATAGCTTGTTTTGATTTGCAAATAAGAAACTCTTTTCACACTGTGAGATGTCCAGGTCGTAACGGTGGCCAAGTTCCAAGGAGGTGGTGCATTTAACGTAATTCCAGATTCTGTTACAATAGGAGGCACTTTCCGAGCTTTCTTGAAGGATAGCATGGTTCAACTCAAGCAACGGATTAAAGAGGTATAGTAATTTGAGTTGCAAAATGAACAATCTCATAAAGTTCTTCAGCTTGTGTTCTTTTCCTAAAAAAAaTCATTAAGCATCGGACATGATTTTTTTtAGTATTACTGTTAACATAAAGTTCTTTGAGTTAGTTGAAATGTAATGTTTGAGACTATCAATGTTACCATCCTAATATGTTAATCTTTGACATGTGGTGGTCTCATTTGGTTCTTTTATAGGTTATCACTGGACAAGCTATCGTGCATAGGTGCAATGCAACTGTCGACTTTTTGGAAAATGATAAACCCATTTTCCCTCCAACTGTGAATGATAAAGAGTTGCATGAACACTTCCAAAATGTTGCAGGAGATATGCTTGGCATTGACAAGGTCAAGGATATGCAACCAGTTATGGGATCTGAAGATTTTTCATTTTACCAAGAGATGATCCCAGGGTACTTCTTCTTTCTTGGAATGGAGAATGAAACATCTGGTCACCTCGATTCTGTGCATTCCCCTTACTTTCGTATCAACGAAGACGCTCTCCCTTATGGCGCAGCTCTTCATGCATCATTGGCTACAAGGTATCTTCTTGAACTCCAGTCAAAAGTTAATTCGCCAGAAGAAAAACGTCATGATGAATTGTAAGTTGCCTATGGACCTGCTCGATCATATGTTTGGATCAGGTTAAAGGGGTGTAAAACATAATCAAAATCTCATTGAGCTGCTACTTAATGTCAATCCCTTACCACATCTGTATTCTGTTCTTTCCATTTAAGCTCATCTTATTTTACCTACCAGAAATCAATCAGCCCAAATGTTCCAAGAAAGTGAAGCCTTTGCACGTTTCCTTTTATTGTGATAGATATCTCTTACAAATAACCATGATCATGGGCACAAGAAGTCTTGTATTACTCCAATTCTTGGGGCTAGATATTCAGTATGTAAATGAGATACCGTGAAGATTCACGTTTAAAAGATTTCTTCTGACTAACAAATTATGTTAATATACTGGTTTTGAATGATTGGCGTCGATCTTGGTAAAGAATAATCATGTAAAATGGCCGAAGTGAGTTTGCCTTTTCACTTGAAGGTTTGATCTTCTTATTGTAAAACAAAATGTAAAATGAATAATAGCTGATGGGACAACGACCATAATTTTCCCTCTTTTAATTTTATTAAAGCAAAAGCTGAGAACAATAGCTTTCCACTTTCACATTCTTTTATATTGCAGGCCCATATTTGAAGAACCTAGTACC

mRNA sequence

TCTTTTTGGACATTACATTACATTGAGCTTTTAAGCTTTCTCTTCATCGCCTCTTTTCTTCTTCCCCTGTGGCTCTTCTCATTCCTATCCCCACCCAGCAATACCCACTTCTCAATTCCttctcttttttttttttcttttttttcttttttACCCATTTTAACTTTTCAATTTCTTGTTTCTTCACTATATGCATTTAATCATGGCTTCCCTCAGGTGGATTTCTTGGATTTTGATCGCACATTTGTTTGTTTCAACATTTGTGCACTCAGATTCTTCCTCCCTCTCAAATAATCCCCAAGAAATTCCTAAAAAGTTCCTCCAATTTGCTAAGGAATCTGAGCTGTTTGATTGGATGGTGGGCATCCGTAGGAAAATTCACGAGAACCCAGAACTTGGTTTTGAGGAATTCGAGACCAGTAAGCTGATTAGAACGGAATTAGATAAATTGGGCATTTCTTATAAATACCCAGTTGCCAGTACTGGAGTTATCGGATTTGTTGGTTCTGGTCAGCCCCCTTTTGTGGCTATTCGAGCAGATATGGATGCTCTTCCTATGCAGGAATTGGTGGAGTGGGAGCATAAGAGTAAGGTTCCTGGGAAGATGCACGCTTGTGGTCACGATGCTCATGTTGCGATGGTCTTGGGTGCTGCAAAGATCCTTCAAAAGCATAGCGAGGAGTTGAAAGGGACAGTTGTACTAGTTTTCCAACCAGCTGAAGAAGGAGGTGGAGGTGCTATGAAAATAATAGAAGCTGGAGTGTTGGATAATGTCAATGCTATCTTTGGGTTGCATATTGTTCATAATATACCTATCGGTAAAGTGGCTGGACGCTCAGGACCCCTGTTAGCTGGAAGTGCCTTTTTTGAAGCAGTAATCAGTGGAAAAGGAGGCCATGCTGCCATTCCTCAGCATTCTATAGACCCAATTTTGGCTGCATCTAACGTTATTGTGAGCTTACAACATCTTGTTTCAAGGGAAGCTGATCCACTAGATTCACAGGTCGTAACGGTGGCCAAGTTCCAAGGAGGTGGTGCATTTAACGTAATTCCAGATTCTGTTACAATAGGAGGCACTTTCCGAGCTTTCTTGAAGGATAGCATGGTTCAACTCAAGCAACGGATTAAAGAGGTTATCACTGGACAAGCTATCGTGCATAGGTGCAATGCAACTGTCGACTTTTTGGAAAATGATAAACCCATTTTCCCTCCAACTGTGAATGATAAAGAGTTGCATGAACACTTCCAAAATGTTGCAGGAGATATGCTTGGCATTGACAAGGTCAAGGATATGCAACCAGTTATGGGATCTGAAGATTTTTCATTTTACCAAGAGATGATCCCAGGGTACTTCTTCTTTCTTGGAATGGAGAATGAAACATCTGGTCACCTCGATTCTGTGCATTCCCCTTACTTTCGTATCAACGAAGACGCTCTCCCTTATGGCGCAGCTCTTCATGCATCATTGGCTACAAGGTATCTTCTTGAACTCCAGTCAAAAGTTAATTCGCCAGAAGAAAAACGTCATGATGAATTGTAAGTTGCCTATGGACCTGCTCGATCATATGTTTGGATCAGGTTAAAGGGGTGTAAAACATAATCAAAATCTCATTGAGCTGCTACTTAATGTCAATCCCTTACCACATCTGTATTCTGTTCTTTCCATTTAAGCTCATCTTATTTTACCTACCAGAAATCAATCAGCCCAAATGTTCCAAGAAAGTGAAGCCTTTGCACGTTTCCTTTTATTGTGATAGATATCTCTTACAAATAACCATGATCATGGGCACAAGAAGTCTTGTATTACTCCAATTCTTGGGGCTAGATATTCAGTATGTAAATGAGATACCGTGAAGATTCACGTTTAAAAGATTTCTTCTGACTAACAAATTATGTTAATATACTGGTTTTGAATGATTGGCGTCGATCTTGGTAAAGAATAATCATGTAAAATGGCCGAAGTGAGTTTGCCTTTTCACTTGAAGGTTTGATCTTCTTATTGTAAAACAAAATGTAAAATGAATAATAGCTGATGGGACAACGACCATAATTTTCCCTCTTTTAATTTTATTAAAGCAAAAGCTGAGAACAATAGCTTTCCACTTTCACATTCTTTTATATTGCAGGCCCATATTTGAAGAACCTAGTACC

Coding sequence (CDS)

ATGCATTTAATCATGGCTTCCCTCAGGTGGATTTCTTGGATTTTGATCGCACATTTGTTTGTTTCAACATTTGTGCACTCAGATTCTTCCTCCCTCTCAAATAATCCCCAAGAAATTCCTAAAAAGTTCCTCCAATTTGCTAAGGAATCTGAGCTGTTTGATTGGATGGTGGGCATCCGTAGGAAAATTCACGAGAACCCAGAACTTGGTTTTGAGGAATTCGAGACCAGTAAGCTGATTAGAACGGAATTAGATAAATTGGGCATTTCTTATAAATACCCAGTTGCCAGTACTGGAGTTATCGGATTTGTTGGTTCTGGTCAGCCCCCTTTTGTGGCTATTCGAGCAGATATGGATGCTCTTCCTATGCAGGAATTGGTGGAGTGGGAGCATAAGAGTAAGGTTCCTGGGAAGATGCACGCTTGTGGTCACGATGCTCATGTTGCGATGGTCTTGGGTGCTGCAAAGATCCTTCAAAAGCATAGCGAGGAGTTGAAAGGGACAGTTGTACTAGTTTTCCAACCAGCTGAAGAAGGAGGTGGAGGTGCTATGAAAATAATAGAAGCTGGAGTGTTGGATAATGTCAATGCTATCTTTGGGTTGCATATTGTTCATAATATACCTATCGGTAAAGTGGCTGGACGCTCAGGACCCCTGTTAGCTGGAAGTGCCTTTTTTGAAGCAGTAATCAGTGGAAAAGGAGGCCATGCTGCCATTCCTCAGCATTCTATAGACCCAATTTTGGCTGCATCTAACGTTATTGTGAGCTTACAACATCTTGTTTCAAGGGAAGCTGATCCACTAGATTCACAGGTCGTAACGGTGGCCAAGTTCCAAGGAGGTGGTGCATTTAACGTAATTCCAGATTCTGTTACAATAGGAGGCACTTTCCGAGCTTTCTTGAAGGATAGCATGGTTCAACTCAAGCAACGGATTAAAGAGGTTATCACTGGACAAGCTATCGTGCATAGGTGCAATGCAACTGTCGACTTTTTGGAAAATGATAAACCCATTTTCCCTCCAACTGTGAATGATAAAGAGTTGCATGAACACTTCCAAAATGTTGCAGGAGATATGCTTGGCATTGACAAGGTCAAGGATATGCAACCAGTTATGGGATCTGAAGATTTTTCATTTTACCAAGAGATGATCCCAGGGTACTTCTTCTTTCTTGGAATGGAGAATGAAACATCTGGTCACCTCGATTCTGTGCATTCCCCTTACTTTCGTATCAACGAAGACGCTCTCCCTTATGGCGCAGCTCTTCATGCATCATTGGCTACAAGGTATCTTCTTGAACTCCAGTCAAAAGTTAATTCGCCAGAAGAAAAACGTCATGATGAATTGTAA

Protein sequence

MHLIMASLRWISWILIAHLFVSTFVHSDSSSLSNNPQEIPKKFLQFAKESELFDWMVGIRRKIHENPELGFEEFETSKLIRTELDKLGISYKYPVASTGVIGFVGSGQPPFVAIRADMDALPMQELVEWEHKSKVPGKMHACGHDAHVAMVLGAAKILQKHSEELKGTVVLVFQPAEEGGGGAMKIIEAGVLDNVNAIFGLHIVHNIPIGKVAGRSGPLLAGSAFFEAVISGKGGHAAIPQHSIDPILAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFLKDSMVQLKQRIKEVITGQAIVHRCNATVDFLENDKPIFPPTVNDKELHEHFQNVAGDMLGIDKVKDMQPVMGSEDFSFYQEMIPGYFFFLGMENETSGHLDSVHSPYFRINEDALPYGAALHASLATRYLLELQSKVNSPEEKRHDEL*
BLAST of Cucsa.341380 vs. Swiss-Prot
Match: ILL4_ARATH (IAA-amino acid hydrolase ILR1-like 4 OS=Arabidopsis thaliana GN=ILL4 PE=1 SV=2)

HSP 1 Score: 646.4 bits (1666), Expect = 2.4e-184
Identity = 310/445 (69.66%), Postives = 377/445 (84.72%), Query Frame = 1

Query: 5   MASLRWISWILIAHLFVSTFVHSDSSSLSNNPQEIPKKFLQFAKESELFDWMVGIRRKIH 64
           M+  +W+S++LI HL   T +   S+ LS    +IP KFL  AK ++ FDWMVGIRR+IH
Sbjct: 1   MSFFKWVSFVLILHLLNPTLISCSSNGLS----QIPSKFLTLAKRNDFFDWMVGIRRRIH 60

Query: 65  ENPELGFEEFETSKLIRTELDKLGISYKYPVASTGVIGFVGSGQPPFVAIRADMDALPMQ 124
           ENPELG+EE ETSKL+R EL+K+G+SYKYPVA TGV+G+VG+G  PFVA+RADMDAL MQ
Sbjct: 61  ENPELGYEEVETSKLVRAELEKMGVSYKYPVAVTGVVGYVGTGHAPFVALRADMDALAMQ 120

Query: 125 ELVEWEHKSKVPGKMHACGHDAHVAMVLGAAKILQKHSEELKGTVVLVFQPAEEGGGGAM 184
           E+VEWEHKSKVPGKMHACGHDAH  M+LGAAK+L++H EEL+GTVVLVFQPAEEGGGGA 
Sbjct: 121 EMVEWEHKSKVPGKMHACGHDAHTTMLLGAAKLLKEHEEELQGTVVLVFQPAEEGGGGAK 180

Query: 185 KIIEAGVLDNVNAIFGLHIVHNIPIGKVAGRSGPLLAGSAFFEAVISGKGGHAAIPQHSI 244
           KI+EAGVL+NV+AIFGLH+ + + +G+V+ R GP+LAGS FF+A ISGKGGHAA+PQH+I
Sbjct: 181 KIVEAGVLENVSAIFGLHVTNQLALGQVSSREGPMLAGSGFFKAKISGKGGHAALPQHTI 240

Query: 245 DPILAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFLKDS 304
           DPILAASNVIVSLQHLVSREADPLDSQVVTVAKF+GGGAFNVIPDSVTIGGTFRAF   S
Sbjct: 241 DPILAASNVIVSLQHLVSREADPLDSQVVTVAKFEGGGAFNVIPDSVTIGGTFRAFSTKS 300

Query: 305 MVQLKQRIKEVITGQAIVHRCNATVDFLENDKPIFPPTVNDKELHEHFQNVAGDMLGIDK 364
            +QLK+RI++VIT QA V+ CNATVDF+E +KP FPPTVNDK LH+ F+NV+GDMLGI+ 
Sbjct: 301 FMQLKKRIEQVITRQASVNMCNATVDFIEEEKPFFPPTVNDKALHQFFKNVSGDMLGIEN 360

Query: 365 VKDMQPVMGSEDFSFYQEMIPGYFFFLGMENETSGHLDSVHSPYFRINEDALPYGAALHA 424
             +MQP+MGSEDFSFYQ+ IPG+F F+GM+N+    + S HSPYF +NE+ LPYGA+LHA
Sbjct: 361 YVEMQPLMGSEDFSFYQQAIPGHFSFVGMQNKARSPMASPHSPYFEVNEELLPYGASLHA 420

Query: 425 SLATRYLLELQSKVNSPEEKRHDEL 450
           S+ATRYLLEL++   +   K+ DEL
Sbjct: 421 SMATRYLLELKASTLNKSNKK-DEL 440

BLAST of Cucsa.341380 vs. Swiss-Prot
Match: ILL1_ORYSI (IAA-amino acid hydrolase ILR1-like 1 OS=Oryza sativa subsp. indica GN=ILL1 PE=2 SV=1)

HSP 1 Score: 587.8 bits (1514), Expect = 1.0e-166
Identity = 291/411 (70.80%), Postives = 336/411 (81.75%), Query Frame = 1

Query: 40  PKKFLQFAKESELFDWMVGIRRKIHENPELGFEEFETSKLIRTELDKLGISYKYPVASTG 99
           P   L+ AKE+E   WMVG+RR+IHENPELG+EEF TS+L+R ELD LGI Y++P A TG
Sbjct: 33  PAGLLRRAKEAEFAGWMVGLRRRIHENPELGYEEFATSELVRRELDALGIPYRHPFAVTG 92

Query: 100 VIGFVGSGQPPFVAIRADMDALPMQELVEWEHKSKVPGKMHACGHDAHVAMVLGAAKILQ 159
           V+  VG+G PPFVA+RADMDALPMQE VEWEHKSKVPGKMH CGHDAHVAM+LG+A+ILQ
Sbjct: 93  VVATVGTGGPPFVALRADMDALPMQESVEWEHKSKVPGKMHGCGHDAHVAMLLGSARILQ 152

Query: 160 KHSEELKGTVVLVFQPAEEGGGGAMKIIEAGVLDNVNAIFGLHIVHNIPIGKVAGRSGPL 219
           +H +ELKGTVVLVFQPAEEGGGGA K+I+ G ++N+ AIFG+H+   +PIG VA R GP+
Sbjct: 153 EHRDELKGTVVLVFQPAEEGGGGAKKMIDDGTVENIEAIFGVHVADVVPIGVVASRPGPV 212

Query: 220 LAGSAFFEAVISGKGGHAAIPQHSIDPILAASNVIVSLQHLVSREADPLDSQVVTVAKFQ 279
           +AGS FFEAVISGKGGHAA+P H+IDPILAASNVIVSLQ LVSREADPLDSQVVTV KFQ
Sbjct: 213 MAGSGFFEAVISGKGGHAALPHHTIDPILAASNVIVSLQQLVSREADPLDSQVVTVGKFQ 272

Query: 280 GGGAFNVIPDSVTIGGTFRAFLKDSMVQLKQRIKEVITGQAIVHRCNATVDFLENDKPIF 339
           GGGAFNVIPDSVTIGGTFRAFLK+S  QLKQRI+EVI  QA V RCNA VDFL+ D+P F
Sbjct: 273 GGGAFNVIPDSVTIGGTFRAFLKESFNQLKQRIEEVIVSQASVQRCNAVVDFLDKDRPFF 332

Query: 340 PPTVNDKELHEHFQNVAGDMLGIDKVKDMQPVMGSEDFSFYQEMIPG-YFFFLGMENETS 399
           PPT+N   LH+ F  VA +M+G   V+D QP+MG+EDF+FY + IP  Y++FLGM NET 
Sbjct: 333 PPTINSAGLHDFFVKVASEMVGPKNVRDKQPLMGAEDFAFYADAIPATYYYFLGMYNETR 392

Query: 400 GHLDSVHSPYFRINEDALPYGAALHASLATRYLLELQSKVNSPEEKRHDEL 450
           G     HSPYF INEDALPYGAAL ASLATRYLLE Q      + K HDEL
Sbjct: 393 GPQAPHHSPYFTINEDALPYGAALQASLATRYLLEHQPPTTG-KAKAHDEL 442

BLAST of Cucsa.341380 vs. Swiss-Prot
Match: ILL5_ARATH (IAA-amino acid hydrolase ILR1-like 5 OS=Arabidopsis thaliana GN=ILL5 PE=3 SV=1)

HSP 1 Score: 587.4 bits (1513), Expect = 1.3e-166
Identity = 288/438 (65.75%), Postives = 355/438 (81.05%), Query Frame = 1

Query: 5   MASLRWISWILIAHLFVSTFVHSDSSSLSNNPQEIPKKFLQFAKESELFDWMVGIRRKIH 64
           M+  + +S++LI HL  S  +   S+ LS    +IPK FL  AK  + FDWMVGIRR+IH
Sbjct: 1   MSFCKLVSFVLILHLLNSCLISCSSNDLS----QIPKNFLSLAKREDFFDWMVGIRRRIH 60

Query: 65  ENPELGFEEFETSKLIRTELDKLGISYKYPVASTGVIGFVGSGQPPFVAIRADMDALPMQ 124
           ENPELG+EE ETSKL++TELDK+G+SYK PVA TGVIG+VG+G  PFVA+RADMDALP+Q
Sbjct: 61  ENPELGYEEVETSKLVKTELDKMGVSYKNPVAVTGVIGYVGTGHAPFVALRADMDALPIQ 120

Query: 125 ELVEWEHKSKVPGKMHACGHDAHVAMVLGAAKILQKHSEELKGTVVLVFQPAEEGGGGAM 184
           E+VEWEHKSK+PGKMHACGHDAH  M+LGAAK+L++H EEL+GTV+LVFQPAEEGG GA 
Sbjct: 121 EMVEWEHKSKIPGKMHACGHDAHTTMLLGAAKLLKEHQEELQGTVILVFQPAEEGGAGAK 180

Query: 185 KIIEAGVLDNVNAIFGLHIVHNIPIGKVAGRSGPLLAGSAFFEAVISGKGGHAAIPQHSI 244
           KI+EAGVL+NV AIFGLH+ + + +G+++ R G L+AGS  F+A ISGKGGHAA+PQ +I
Sbjct: 181 KIVEAGVLENVGAIFGLHVSNLLGLGQLSSREGLLMAGSGRFKATISGKGGHAALPQFAI 240

Query: 245 DPILAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFLKDS 304
           DP+LAASNVI+SLQHLVSREADPLDSQVVTVA F+G  AFNVIPDSVTIGGTFRA L  S
Sbjct: 241 DPVLAASNVILSLQHLVSREADPLDSQVVTVATFEGSDAFNVIPDSVTIGGTFRALLPKS 300

Query: 305 MVQLKQRIKEVITGQAIVHRCNATVDFLENDKPIFPPTVNDKELHEHFQNVAGDMLGIDK 364
             QLKQRI +VIT QA V+ CNATVDFLE++ P FPPTVN+K LH  ++NV+ DMLGI+ 
Sbjct: 301 FEQLKQRIVQVITTQASVNMCNATVDFLEDETPPFPPTVNNKTLHLFYKNVSVDMLGIEN 360

Query: 365 VKDMQPVMGSEDFSFYQEMIPGYFFFLGMENETSGHLDSVHSPYFRINEDALPYGAALHA 424
             +  PVM SEDF+FYQ+ IPG+F F+GM+N++   + + HSP+F +NE+ LPYGA+L A
Sbjct: 361 YVETLPVMVSEDFAFYQQAIPGHFSFVGMQNKSHSPMANPHSPFFEVNEELLPYGASLLA 420

Query: 425 SLATRYLLELQSKVNSPE 443
           SLATRYLL+  S  N  E
Sbjct: 421 SLATRYLLDSSSSPNKDE 434

BLAST of Cucsa.341380 vs. Swiss-Prot
Match: ILL1_ORYSJ (IAA-amino acid hydrolase ILR1-like 1 OS=Oryza sativa subsp. japonica GN=ILL1 PE=2 SV=1)

HSP 1 Score: 585.9 bits (1509), Expect = 3.8e-166
Identity = 290/411 (70.56%), Postives = 335/411 (81.51%), Query Frame = 1

Query: 40  PKKFLQFAKESELFDWMVGIRRKIHENPELGFEEFETSKLIRTELDKLGISYKYPVASTG 99
           P   L+ AKE+E   WMVG+RR+IHENPELG+EEF TS+L+R ELD LGI Y++P A TG
Sbjct: 33  PAGLLRRAKEAEFAGWMVGLRRRIHENPELGYEEFATSELVRRELDALGIPYRHPFAVTG 92

Query: 100 VIGFVGSGQPPFVAIRADMDALPMQELVEWEHKSKVPGKMHACGHDAHVAMVLGAAKILQ 159
           V+  VG+G PPFVA+RADMDALPMQE VEWEHKSKVPGKMH CGHDAHVAM+LG+A+ILQ
Sbjct: 93  VVATVGTGGPPFVALRADMDALPMQESVEWEHKSKVPGKMHGCGHDAHVAMLLGSARILQ 152

Query: 160 KHSEELKGTVVLVFQPAEEGGGGAMKIIEAGVLDNVNAIFGLHIVHNIPIGKVAGRSGPL 219
           +H +ELKGTVVLVFQPAEEGGGGA K+I+ G ++N+ AIFG+H+   +PIG VA R GP+
Sbjct: 153 EHRDELKGTVVLVFQPAEEGGGGAKKMIDDGAVENIEAIFGVHVADVVPIGVVASRPGPV 212

Query: 220 LAGSAFFEAVISGKGGHAAIPQHSIDPILAASNVIVSLQHLVSREADPLDSQVVTVAKFQ 279
           +AGS FFEAVISGKGGHAA+P H+IDPILAASNVIVSLQ LVSREADPLDSQVVTV KFQ
Sbjct: 213 MAGSGFFEAVISGKGGHAALPHHTIDPILAASNVIVSLQQLVSREADPLDSQVVTVGKFQ 272

Query: 280 GGGAFNVIPDSVTIGGTFRAFLKDSMVQLKQRIKEVITGQAIVHRCNATVDFLENDKPIF 339
           GGGAFNVIPDSVTIGGTFRAFLK+S  QLKQRI+EVI  QA V RCNA VDFL+ D+P F
Sbjct: 273 GGGAFNVIPDSVTIGGTFRAFLKESFNQLKQRIEEVIVSQASVQRCNAVVDFLDKDRPFF 332

Query: 340 PPTVNDKELHEHFQNVAGDMLGIDKVKDMQPVMGSEDFSFYQEMIPG-YFFFLGMENETS 399
           PPT+N   LH+ F  VA +M+G   V+D QP+MG+EDF+FY + IP  Y++FLGM NET 
Sbjct: 333 PPTINSAGLHDFFVKVASEMVGPKNVRDKQPLMGAEDFAFYADAIPATYYYFLGMYNETR 392

Query: 400 GHLDSVHSPYFRINEDALPYGAALHASLATRYLLELQSKVNSPEEKRHDEL 450
           G     HSPYF INEDALPYGAAL ASLA RYLLE Q      + K HDEL
Sbjct: 393 GPQAPHHSPYFTINEDALPYGAALQASLAARYLLEHQPPTTG-KAKAHDEL 442

BLAST of Cucsa.341380 vs. Swiss-Prot
Match: ILL2_ARATH (IAA-amino acid hydrolase ILR1-like 2 OS=Arabidopsis thaliana GN=ILL2 PE=1 SV=2)

HSP 1 Score: 540.8 bits (1392), Expect = 1.4e-152
Identity = 264/413 (63.92%), Postives = 327/413 (79.18%), Query Frame = 1

Query: 25  VHSDSSSLSNNPQEIPKKFLQFAKESELFDWMVGIRRKIHENPELGFEEFETSKLIRTEL 84
           V S+S  ++ +  +I  K L+FAK  E+FDWMV IRRKIHENPELG+EE ETSKLIR+EL
Sbjct: 20  VSSESPWIAEDTSQIQTKLLEFAKSPEVFDWMVKIRRKIHENPELGYEELETSKLIRSEL 79

Query: 85  DKLGISYKYPVASTGVIGFVGSGQPPFVAIRADMDALPMQELVEWEHKSKVPGKMHACGH 144
           + +GI Y+YPVA TGVIG++G+G+PPFVA+RADMDALP+QE VEWEHKSK+ GKMHACGH
Sbjct: 80  ELIGIKYRYPVAITGVIGYIGTGEPPFVALRADMDALPIQEGVEWEHKSKIAGKMHACGH 139

Query: 145 DAHVAMVLGAAKILQKHSEELKGTVVLVFQPAEEGGGGAMKIIEAGVLDNVNAIFGLHIV 204
           D HV M+LGAAKIL +H   L+GTVVL+FQPAEEG  GA K+ E G L NV AIFG+H+ 
Sbjct: 140 DGHVTMLLGAAKILHEHRHHLQGTVVLIFQPAEEGLSGAKKMREEGALKNVEAIFGIHLS 199

Query: 205 HNIPIGKVAGRSGPLLAGSAFFEAVISGKGGHAAIPQHSIDPILAASNVIVSLQHLVSRE 264
             IP GK A R+G  LAG+  FEAVI+GKGGHAAIPQH+IDP++AAS++++SLQ LVSRE
Sbjct: 200 ARIPFGKAASRAGSFLAGAGVFEAVITGKGGHAAIPQHTIDPVVAASSIVLSLQQLVSRE 259

Query: 265 ADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFLKDSMVQLKQRIKEVITGQAIVHR 324
            DPLDS+VVTV+K  GG AFNVIPDS+TIGGT RAF      QL+QR+KEVIT QA VHR
Sbjct: 260 TDPLDSKVVTVSKVNGGNAFNVIPDSITIGGTLRAF--TGFTQLQQRVKEVITKQAAVHR 319

Query: 325 CNATVDFLENDKPIFPPTVNDKELHEHFQNVAGDMLGIDKVKDMQPVMGSEDFSFYQEMI 384
           CNA+V+   N +   PPTVN+K+L++ F+ V  D+LG +   +  PVMGSEDFS++ E I
Sbjct: 320 CNASVNLTPNGREPMPPTVNNKDLYKQFKKVVRDLLGQEAFVEAAPVMGSEDFSYFAETI 379

Query: 385 PGYFFFLGMENETSGHLDSVHSPYFRINEDALPYGAALHASLATRYLLELQSK 438
           PG+F  LGM++ET+G+  S HSP +RINED LPYGAA+HAS+A +YL E  SK
Sbjct: 380 PGHFSLLGMQDETNGYASS-HSPLYRINEDVLPYGAAIHASMAVQYLKEKASK 429

BLAST of Cucsa.341380 vs. TrEMBL
Match: A0A0A0LFU9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G850490 PE=4 SV=1)

HSP 1 Score: 899.4 bits (2323), Expect = 1.7e-258
Identity = 449/449 (100.00%), Postives = 449/449 (100.00%), Query Frame = 1

Query: 1   MHLIMASLRWISWILIAHLFVSTFVHSDSSSLSNNPQEIPKKFLQFAKESELFDWMVGIR 60
           MHLIMASLRWISWILIAHLFVSTFVHSDSSSLSNNPQEIPKKFLQFAKESELFDWMVGIR
Sbjct: 1   MHLIMASLRWISWILIAHLFVSTFVHSDSSSLSNNPQEIPKKFLQFAKESELFDWMVGIR 60

Query: 61  RKIHENPELGFEEFETSKLIRTELDKLGISYKYPVASTGVIGFVGSGQPPFVAIRADMDA 120
           RKIHENPELGFEEFETSKLIRTELDKLGISYKYPVASTGVIGFVGSGQPPFVAIRADMDA
Sbjct: 61  RKIHENPELGFEEFETSKLIRTELDKLGISYKYPVASTGVIGFVGSGQPPFVAIRADMDA 120

Query: 121 LPMQELVEWEHKSKVPGKMHACGHDAHVAMVLGAAKILQKHSEELKGTVVLVFQPAEEGG 180
           LPMQELVEWEHKSKVPGKMHACGHDAHVAMVLGAAKILQKHSEELKGTVVLVFQPAEEGG
Sbjct: 121 LPMQELVEWEHKSKVPGKMHACGHDAHVAMVLGAAKILQKHSEELKGTVVLVFQPAEEGG 180

Query: 181 GGAMKIIEAGVLDNVNAIFGLHIVHNIPIGKVAGRSGPLLAGSAFFEAVISGKGGHAAIP 240
           GGAMKIIEAGVLDNVNAIFGLHIVHNIPIGKVAGRSGPLLAGSAFFEAVISGKGGHAAIP
Sbjct: 181 GGAMKIIEAGVLDNVNAIFGLHIVHNIPIGKVAGRSGPLLAGSAFFEAVISGKGGHAAIP 240

Query: 241 QHSIDPILAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAF 300
           QHSIDPILAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAF
Sbjct: 241 QHSIDPILAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAF 300

Query: 301 LKDSMVQLKQRIKEVITGQAIVHRCNATVDFLENDKPIFPPTVNDKELHEHFQNVAGDML 360
           LKDSMVQLKQRIKEVITGQAIVHRCNATVDFLENDKPIFPPTVNDKELHEHFQNVAGDML
Sbjct: 301 LKDSMVQLKQRIKEVITGQAIVHRCNATVDFLENDKPIFPPTVNDKELHEHFQNVAGDML 360

Query: 361 GIDKVKDMQPVMGSEDFSFYQEMIPGYFFFLGMENETSGHLDSVHSPYFRINEDALPYGA 420
           GIDKVKDMQPVMGSEDFSFYQEMIPGYFFFLGMENETSGHLDSVHSPYFRINEDALPYGA
Sbjct: 361 GIDKVKDMQPVMGSEDFSFYQEMIPGYFFFLGMENETSGHLDSVHSPYFRINEDALPYGA 420

Query: 421 ALHASLATRYLLELQSKVNSPEEKRHDEL 450
           ALHASLATRYLLELQSKVNSPEEKRHDEL
Sbjct: 421 ALHASLATRYLLELQSKVNSPEEKRHDEL 449

BLAST of Cucsa.341380 vs. TrEMBL
Match: A0A061GAV6_THECC (Peptidase M20/M25/M40 family protein OS=Theobroma cacao GN=TCM_028981 PE=4 SV=1)

HSP 1 Score: 695.3 bits (1793), Expect = 5.0e-197
Identity = 349/448 (77.90%), Postives = 393/448 (87.72%), Query Frame = 1

Query: 5   MASLRWISWILIAHLFVSTFVHSDSS-SLSNNPQEIPKKFLQFAKESELFDWMVGIRRKI 64
           M+  +++S++L+ HL  +T V S SS S SN   EIPKKFL FAK  EL DW+VGIRRKI
Sbjct: 1   MSFSKFVSFVLMLHLLNTTVVLSSSSLSSSNGWSEIPKKFLHFAKGQELVDWIVGIRRKI 60

Query: 65  HENPELGFEEFETSKLIRTELDKLGISYKYPVASTGVIGFVGSGQPPFVAIRADMDALPM 124
           HENPELG+EEFETSKLIR ELDK+GI YKYPV+ TGV+G+VG+G+PPFVAIRADMDAL M
Sbjct: 61  HENPELGYEEFETSKLIREELDKMGIPYKYPVSVTGVVGYVGTGKPPFVAIRADMDALAM 120

Query: 125 QELVEWEHKSKVPGKMHACGHDAHVAMVLGAAKILQKHSEELKGTVVLVFQPAEEGGGGA 184
           +ELVE+EHKSK PGKMHACGHDAHV+M+LGAAKIL++H EELKGTV+LVFQPAEEGGGGA
Sbjct: 121 EELVEFEHKSKNPGKMHACGHDAHVSMLLGAAKILKEHLEELKGTVLLVFQPAEEGGGGA 180

Query: 185 MKIIEAGVLDNVNAIFGLHIVHNIPIGKVAGRSGPLLAGSAFFEAVISGKGGHAAIPQHS 244
            K+++AGVL NV+AIFGLH+V + PIG VA R GPLLAGS FFEAVISGKGGHAAIPQHS
Sbjct: 181 KKMLDAGVLKNVDAIFGLHVVPDQPIGTVASRPGPLLAGSGFFEAVISGKGGHAAIPQHS 240

Query: 245 IDPILAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFLKD 304
           IDPILAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAF K+
Sbjct: 241 IDPILAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFSKE 300

Query: 305 SMVQLKQRIKEVITGQAIVHRCNATVDFLENDK--PIFPPTVNDKELHEHFQNVAGDMLG 364
           S+ QLKQRI+EVI GQA V RC+ATVDF EN+K  PIFPPTVN+K+LHEHFQNVAGDMLG
Sbjct: 301 SLTQLKQRIEEVIKGQAAVQRCSATVDFYENEKPVPIFPPTVNNKDLHEHFQNVAGDMLG 360

Query: 365 IDKVKDMQPVMGSEDFSFYQEMIPGYFFFLGMENETSGHLDSVHSPYFRINEDALPYGAA 424
            DKVKDMQP+MGSEDF+FYQE IPGYFF LGM ++T   L SVHSPYF INED LPYGAA
Sbjct: 361 ADKVKDMQPLMGSEDFAFYQEAIPGYFFMLGMRDDTGPQLKSVHSPYFMINEDVLPYGAA 420

Query: 425 LHASLATRYLLELQSKVNSPEEKRHDEL 450
           LHASLA RYLL  + K +SP+E+ HDEL
Sbjct: 421 LHASLAARYLLAAEPKFHSPKEELHDEL 448

BLAST of Cucsa.341380 vs. TrEMBL
Match: D7U044_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_09s0002g02630 PE=4 SV=1)

HSP 1 Score: 694.1 bits (1790), Expect = 1.1e-196
Identity = 340/445 (76.40%), Postives = 388/445 (87.19%), Query Frame = 1

Query: 5   MASLRWISWILIAHLFVSTFVHSDSSSLSNNPQEIPKKFLQFAKESELFDWMVGIRRKIH 64
           M    W+SWI I  LF  T + S+SS  SN    IP  FL FA++ E+ DW+VG+RRKIH
Sbjct: 1   MTYTEWVSWIFILCLFGPTPISSESSLSSN----IPTNFLSFARKQEVVDWLVGVRRKIH 60

Query: 65  ENPELGFEEFETSKLIRTELDKLGISYKYPVASTGVIGFVGSGQPPFVAIRADMDALPMQ 124
           ENPELGFEE ETSKL+R ELDK+GI YKYPVA TGV+GFVG+G+PPFVAIRADMDAL MQ
Sbjct: 61  ENPELGFEEVETSKLVRAELDKMGIPYKYPVAVTGVLGFVGTGEPPFVAIRADMDALAMQ 120

Query: 125 ELVEWEHKSKVPGKMHACGHDAHVAMVLGAAKILQKHSEELKGTVVLVFQPAEEGGGGAM 184
           E+VEWEHKSK+PGKMHACGHD+HVAM+LGAAKILQ+H EEL+GTV+LVFQPAEEGGGGA 
Sbjct: 121 EMVEWEHKSKIPGKMHACGHDSHVAMLLGAAKILQEHREELQGTVILVFQPAEEGGGGAK 180

Query: 185 KIIEAGVLDNVNAIFGLHIVHNIPIGKVAGRSGPLLAGSAFFEAVISGKGGHAAIPQHSI 244
           KI++AGVL+NVNAIFGLH+  ++PIG+VA RSGPLLAGS FFEAVISGKGGHAAIPQHSI
Sbjct: 181 KILDAGVLENVNAIFGLHVSPDLPIGEVASRSGPLLAGSGFFEAVISGKGGHAAIPQHSI 240

Query: 245 DPILAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFLKDS 304
           DPILAASNVIVSLQHLVSREADPL+SQVVTVAKFQGGGAFNVIPDSVTIGGTFRAF K+S
Sbjct: 241 DPILAASNVIVSLQHLVSREADPLESQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFSKES 300

Query: 305 MVQLKQRIKEVITGQAIVHRCNATVDFLENDKPIFPPTVNDKELHEHFQNVAGDMLGIDK 364
           ++QLKQRI+EVIT QA V RCNATVDF E +KP+FP T+N+  LH+HFQNV G+MLG+  
Sbjct: 301 IMQLKQRIEEVITRQAAVQRCNATVDFHEKEKPLFPATINNPNLHKHFQNVVGNMLGVHN 360

Query: 365 VKDMQPVMGSEDFSFYQEMIPGYFFFLGMENETSGHLDSVHSPYFRINEDALPYGAALHA 424
           VKDMQP+MGSEDFSFYQE +PGYFFFLGM++E  G L SVHSP+F+INEDALPYGAALHA
Sbjct: 361 VKDMQPLMGSEDFSFYQEEMPGYFFFLGMKDEALGRLPSVHSPHFKINEDALPYGAALHA 420

Query: 425 SLATRYLLELQSKVNSPEEKRHDEL 450
           SLA  YLLE+Q + +S E K HDEL
Sbjct: 421 SLAATYLLEIQPQPSSREGKHHDEL 441

BLAST of Cucsa.341380 vs. TrEMBL
Match: B9GVN2_POPTR (Putative auxin-amidohydrolase family protein OS=Populus trichocarpa GN=POPTR_0003s04010g PE=4 SV=1)

HSP 1 Score: 693.3 bits (1788), Expect = 1.9e-196
Identity = 337/446 (75.56%), Postives = 385/446 (86.32%), Query Frame = 1

Query: 5   MASLRWISWILIAHLFVSTFVHSDSSSLS-NNPQEIPKKFLQFAKESELFDWMVGIRRKI 64
           M+   W+S        +  F++  S  LS N   +IP +FL +AK+ ELFDWMVG+RRKI
Sbjct: 1   MSLFNWVS--------LGFFLYLLSPILSLNGSSDIPSRFLNYAKKEELFDWMVGVRRKI 60

Query: 65  HENPELGFEEFETSKLIRTELDKLGISYKYPVASTGVIGFVGSGQPPFVAIRADMDALPM 124
           HENPELGFEEFETSKL+R ELDK+G+ YK+P++ TGV+GF+GSG+PPFVA+RADMDAL M
Sbjct: 61  HENPELGFEEFETSKLVRAELDKIGVKYKHPLSVTGVVGFIGSGKPPFVALRADMDALAM 120

Query: 125 QELVEWEHKSKVPGKMHACGHDAHVAMVLGAAKILQKHSEELKGTVVLVFQPAEEGGGGA 184
           QE+VEWE+KSKVPGKMHACGHD+HVAM+LGAAKILQ H EELKGTVVL+FQPAEEGGGGA
Sbjct: 121 QEMVEWEYKSKVPGKMHACGHDSHVAMLLGAAKILQDHREELKGTVVLIFQPAEEGGGGA 180

Query: 185 MKIIEAGVLDNVNAIFGLHIVHNIPIGKVAGRSGPLLAGSAFFEAVISGKGGHAAIPQHS 244
            K+I+ G L+NVNAIFGLH+ + +PIG+VA R GPLLAGS FFEAVISGKGGHAAIPQHS
Sbjct: 181 KKMIDEGALENVNAIFGLHVANKLPIGEVASRHGPLLAGSGFFEAVISGKGGHAAIPQHS 240

Query: 245 IDPILAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFLKD 304
           IDPILAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFLK+
Sbjct: 241 IDPILAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFLKE 300

Query: 305 SMVQLKQRIKEVITGQAIVHRCNATVDFLENDKPIFPPTVNDKELHEHFQNVAGDMLGID 364
           S +QLKQRI+EV+TGQA V RC A ++FLEN+KP FPPT+NDK LH++F+ VA DMLGID
Sbjct: 301 SFMQLKQRIEEVVTGQAAVQRCKAVINFLENEKPFFPPTINDKYLHDYFRIVASDMLGID 360

Query: 365 KVKDMQPVMGSEDFSFYQEMIPGYFFFLGMENETSGHLDSVHSPYFRINEDALPYGAALH 424
           KVKDMQP+MGSEDF+FYQEMIPGYFFF+GM+NET   L S HSPYF INED LPYGAALH
Sbjct: 361 KVKDMQPLMGSEDFAFYQEMIPGYFFFIGMQNETHKQLQSPHSPYFEINEDVLPYGAALH 420

Query: 425 ASLATRYLLELQSKVNSPEEKRHDEL 450
           ASLA RYLLE Q +V  PEE  HDEL
Sbjct: 421 ASLAARYLLEFQPEVTLPEENDHDEL 438

BLAST of Cucsa.341380 vs. TrEMBL
Match: A5BUS2_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_033718 PE=4 SV=1)

HSP 1 Score: 693.0 bits (1787), Expect = 2.5e-196
Identity = 340/445 (76.40%), Postives = 388/445 (87.19%), Query Frame = 1

Query: 5   MASLRWISWILIAHLFVSTFVHSDSSSLSNNPQEIPKKFLQFAKESELFDWMVGIRRKIH 64
           M    W+SWI I  LF  T + S+SS  SN    IP  FL FA++ E+ DW+VG+RRKIH
Sbjct: 1   MTYTEWVSWIFILCLFGPTPISSESSLSSN----IPTNFLSFARKQEVVDWLVGVRRKIH 60

Query: 65  ENPELGFEEFETSKLIRTELDKLGISYKYPVASTGVIGFVGSGQPPFVAIRADMDALPMQ 124
           ENPELGFEE ETSKL+R ELDK+GI YKYPVA TGV+GFVG+G+PPFVAIRADMDAL MQ
Sbjct: 61  ENPELGFEEVETSKLVRAELDKMGIPYKYPVAVTGVLGFVGTGEPPFVAIRADMDALAMQ 120

Query: 125 ELVEWEHKSKVPGKMHACGHDAHVAMVLGAAKILQKHSEELKGTVVLVFQPAEEGGGGAM 184
           E+VEWEHKSK+PGKMHACGHD+HVAM+LGAAKILQ+H EEL+GTV+LVFQPAEEGGGGA 
Sbjct: 121 EMVEWEHKSKIPGKMHACGHDSHVAMLLGAAKILQEHREELQGTVILVFQPAEEGGGGAK 180

Query: 185 KIIEAGVLDNVNAIFGLHIVHNIPIGKVAGRSGPLLAGSAFFEAVISGKGGHAAIPQHSI 244
           KI++AGVL+NVNAIFGLH+  ++PIG+VA RSGPLLAGS FFEAVISGKGGHAAIPQHSI
Sbjct: 181 KILDAGVLENVNAIFGLHVSPDLPIGEVASRSGPLLAGSGFFEAVISGKGGHAAIPQHSI 240

Query: 245 DPILAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFLKDS 304
           DPILAASNVIVSLQHLVSREADPL+SQVVTVAKFQGGGAFNVIPDSVTIGGTFRAF K+S
Sbjct: 241 DPILAASNVIVSLQHLVSREADPLESQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFSKES 300

Query: 305 MVQLKQRIKEVITGQAIVHRCNATVDFLENDKPIFPPTVNDKELHEHFQNVAGDMLGIDK 364
           ++QLKQRI+EVIT QA V RCNATVDF E +KP+FP T+N+  LH+HFQNVAG+MLG+  
Sbjct: 301 IMQLKQRIEEVITRQAAVQRCNATVDFHEKEKPLFPATINNPNLHKHFQNVAGNMLGVHN 360

Query: 365 VKDMQPVMGSEDFSFYQEMIPGYFFFLGMENETSGHLDSVHSPYFRINEDALPYGAALHA 424
           VKDMQP+MGSEDFSFYQE +PGYFFFLGM++E  G L SVHSP+F+INE ALPYGAALHA
Sbjct: 361 VKDMQPLMGSEDFSFYQEEMPGYFFFLGMKDEALGRLPSVHSPHFKINEGALPYGAALHA 420

Query: 425 SLATRYLLELQSKVNSPEEKRHDEL 450
           SLA  YLLE+Q + +S E K HDEL
Sbjct: 421 SLAATYLLEIQPQPSSREGKHHDEL 441

BLAST of Cucsa.341380 vs. TAIR10
Match: AT1G51760.1 (AT1G51760.1 peptidase M20/M25/M40 family protein)

HSP 1 Score: 646.4 bits (1666), Expect = 1.3e-185
Identity = 310/445 (69.66%), Postives = 377/445 (84.72%), Query Frame = 1

Query: 5   MASLRWISWILIAHLFVSTFVHSDSSSLSNNPQEIPKKFLQFAKESELFDWMVGIRRKIH 64
           M+  +W+S++LI HL   T +   S+ LS    +IP KFL  AK ++ FDWMVGIRR+IH
Sbjct: 1   MSFFKWVSFVLILHLLNPTLISCSSNGLS----QIPSKFLTLAKRNDFFDWMVGIRRRIH 60

Query: 65  ENPELGFEEFETSKLIRTELDKLGISYKYPVASTGVIGFVGSGQPPFVAIRADMDALPMQ 124
           ENPELG+EE ETSKL+R EL+K+G+SYKYPVA TGV+G+VG+G  PFVA+RADMDAL MQ
Sbjct: 61  ENPELGYEEVETSKLVRAELEKMGVSYKYPVAVTGVVGYVGTGHAPFVALRADMDALAMQ 120

Query: 125 ELVEWEHKSKVPGKMHACGHDAHVAMVLGAAKILQKHSEELKGTVVLVFQPAEEGGGGAM 184
           E+VEWEHKSKVPGKMHACGHDAH  M+LGAAK+L++H EEL+GTVVLVFQPAEEGGGGA 
Sbjct: 121 EMVEWEHKSKVPGKMHACGHDAHTTMLLGAAKLLKEHEEELQGTVVLVFQPAEEGGGGAK 180

Query: 185 KIIEAGVLDNVNAIFGLHIVHNIPIGKVAGRSGPLLAGSAFFEAVISGKGGHAAIPQHSI 244
           KI+EAGVL+NV+AIFGLH+ + + +G+V+ R GP+LAGS FF+A ISGKGGHAA+PQH+I
Sbjct: 181 KIVEAGVLENVSAIFGLHVTNQLALGQVSSREGPMLAGSGFFKAKISGKGGHAALPQHTI 240

Query: 245 DPILAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFLKDS 304
           DPILAASNVIVSLQHLVSREADPLDSQVVTVAKF+GGGAFNVIPDSVTIGGTFRAF   S
Sbjct: 241 DPILAASNVIVSLQHLVSREADPLDSQVVTVAKFEGGGAFNVIPDSVTIGGTFRAFSTKS 300

Query: 305 MVQLKQRIKEVITGQAIVHRCNATVDFLENDKPIFPPTVNDKELHEHFQNVAGDMLGIDK 364
            +QLK+RI++VIT QA V+ CNATVDF+E +KP FPPTVNDK LH+ F+NV+GDMLGI+ 
Sbjct: 301 FMQLKKRIEQVITRQASVNMCNATVDFIEEEKPFFPPTVNDKALHQFFKNVSGDMLGIEN 360

Query: 365 VKDMQPVMGSEDFSFYQEMIPGYFFFLGMENETSGHLDSVHSPYFRINEDALPYGAALHA 424
             +MQP+MGSEDFSFYQ+ IPG+F F+GM+N+    + S HSPYF +NE+ LPYGA+LHA
Sbjct: 361 YVEMQPLMGSEDFSFYQQAIPGHFSFVGMQNKARSPMASPHSPYFEVNEELLPYGASLHA 420

Query: 425 SLATRYLLELQSKVNSPEEKRHDEL 450
           S+ATRYLLEL++   +   K+ DEL
Sbjct: 421 SMATRYLLELKASTLNKSNKK-DEL 440

BLAST of Cucsa.341380 vs. TAIR10
Match: AT1G51780.1 (AT1G51780.1 IAA-leucine resistant (ILR)-like gene 5)

HSP 1 Score: 587.4 bits (1513), Expect = 7.4e-168
Identity = 288/438 (65.75%), Postives = 355/438 (81.05%), Query Frame = 1

Query: 5   MASLRWISWILIAHLFVSTFVHSDSSSLSNNPQEIPKKFLQFAKESELFDWMVGIRRKIH 64
           M+  + +S++LI HL  S  +   S+ LS    +IPK FL  AK  + FDWMVGIRR+IH
Sbjct: 1   MSFCKLVSFVLILHLLNSCLISCSSNDLS----QIPKNFLSLAKREDFFDWMVGIRRRIH 60

Query: 65  ENPELGFEEFETSKLIRTELDKLGISYKYPVASTGVIGFVGSGQPPFVAIRADMDALPMQ 124
           ENPELG+EE ETSKL++TELDK+G+SYK PVA TGVIG+VG+G  PFVA+RADMDALP+Q
Sbjct: 61  ENPELGYEEVETSKLVKTELDKMGVSYKNPVAVTGVIGYVGTGHAPFVALRADMDALPIQ 120

Query: 125 ELVEWEHKSKVPGKMHACGHDAHVAMVLGAAKILQKHSEELKGTVVLVFQPAEEGGGGAM 184
           E+VEWEHKSK+PGKMHACGHDAH  M+LGAAK+L++H EEL+GTV+LVFQPAEEGG GA 
Sbjct: 121 EMVEWEHKSKIPGKMHACGHDAHTTMLLGAAKLLKEHQEELQGTVILVFQPAEEGGAGAK 180

Query: 185 KIIEAGVLDNVNAIFGLHIVHNIPIGKVAGRSGPLLAGSAFFEAVISGKGGHAAIPQHSI 244
           KI+EAGVL+NV AIFGLH+ + + +G+++ R G L+AGS  F+A ISGKGGHAA+PQ +I
Sbjct: 181 KIVEAGVLENVGAIFGLHVSNLLGLGQLSSREGLLMAGSGRFKATISGKGGHAALPQFAI 240

Query: 245 DPILAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFLKDS 304
           DP+LAASNVI+SLQHLVSREADPLDSQVVTVA F+G  AFNVIPDSVTIGGTFRA L  S
Sbjct: 241 DPVLAASNVILSLQHLVSREADPLDSQVVTVATFEGSDAFNVIPDSVTIGGTFRALLPKS 300

Query: 305 MVQLKQRIKEVITGQAIVHRCNATVDFLENDKPIFPPTVNDKELHEHFQNVAGDMLGIDK 364
             QLKQRI +VIT QA V+ CNATVDFLE++ P FPPTVN+K LH  ++NV+ DMLGI+ 
Sbjct: 301 FEQLKQRIVQVITTQASVNMCNATVDFLEDETPPFPPTVNNKTLHLFYKNVSVDMLGIEN 360

Query: 365 VKDMQPVMGSEDFSFYQEMIPGYFFFLGMENETSGHLDSVHSPYFRINEDALPYGAALHA 424
             +  PVM SEDF+FYQ+ IPG+F F+GM+N++   + + HSP+F +NE+ LPYGA+L A
Sbjct: 361 YVETLPVMVSEDFAFYQQAIPGHFSFVGMQNKSHSPMANPHSPFFEVNEELLPYGASLLA 420

Query: 425 SLATRYLLELQSKVNSPE 443
           SLATRYLL+  S  N  E
Sbjct: 421 SLATRYLLDSSSSPNKDE 434

BLAST of Cucsa.341380 vs. TAIR10
Match: AT5G56660.1 (AT5G56660.1 IAA-leucine resistant (ILR)-like 2)

HSP 1 Score: 540.8 bits (1392), Expect = 7.9e-154
Identity = 264/413 (63.92%), Postives = 327/413 (79.18%), Query Frame = 1

Query: 25  VHSDSSSLSNNPQEIPKKFLQFAKESELFDWMVGIRRKIHENPELGFEEFETSKLIRTEL 84
           V S+S  ++ +  +I  K L+FAK  E+FDWMV IRRKIHENPELG+EE ETSKLIR+EL
Sbjct: 20  VSSESPWIAEDTSQIQTKLLEFAKSPEVFDWMVKIRRKIHENPELGYEELETSKLIRSEL 79

Query: 85  DKLGISYKYPVASTGVIGFVGSGQPPFVAIRADMDALPMQELVEWEHKSKVPGKMHACGH 144
           + +GI Y+YPVA TGVIG++G+G+PPFVA+RADMDALP+QE VEWEHKSK+ GKMHACGH
Sbjct: 80  ELIGIKYRYPVAITGVIGYIGTGEPPFVALRADMDALPIQEGVEWEHKSKIAGKMHACGH 139

Query: 145 DAHVAMVLGAAKILQKHSEELKGTVVLVFQPAEEGGGGAMKIIEAGVLDNVNAIFGLHIV 204
           D HV M+LGAAKIL +H   L+GTVVL+FQPAEEG  GA K+ E G L NV AIFG+H+ 
Sbjct: 140 DGHVTMLLGAAKILHEHRHHLQGTVVLIFQPAEEGLSGAKKMREEGALKNVEAIFGIHLS 199

Query: 205 HNIPIGKVAGRSGPLLAGSAFFEAVISGKGGHAAIPQHSIDPILAASNVIVSLQHLVSRE 264
             IP GK A R+G  LAG+  FEAVI+GKGGHAAIPQH+IDP++AAS++++SLQ LVSRE
Sbjct: 200 ARIPFGKAASRAGSFLAGAGVFEAVITGKGGHAAIPQHTIDPVVAASSIVLSLQQLVSRE 259

Query: 265 ADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFLKDSMVQLKQRIKEVITGQAIVHR 324
            DPLDS+VVTV+K  GG AFNVIPDS+TIGGT RAF      QL+QR+KEVIT QA VHR
Sbjct: 260 TDPLDSKVVTVSKVNGGNAFNVIPDSITIGGTLRAF--TGFTQLQQRVKEVITKQAAVHR 319

Query: 325 CNATVDFLENDKPIFPPTVNDKELHEHFQNVAGDMLGIDKVKDMQPVMGSEDFSFYQEMI 384
           CNA+V+   N +   PPTVN+K+L++ F+ V  D+LG +   +  PVMGSEDFS++ E I
Sbjct: 320 CNASVNLTPNGREPMPPTVNNKDLYKQFKKVVRDLLGQEAFVEAAPVMGSEDFSYFAETI 379

Query: 385 PGYFFFLGMENETSGHLDSVHSPYFRINEDALPYGAALHASLATRYLLELQSK 438
           PG+F  LGM++ET+G+  S HSP +RINED LPYGAA+HAS+A +YL E  SK
Sbjct: 380 PGHFSLLGMQDETNGYASS-HSPLYRINEDVLPYGAAIHASMAVQYLKEKASK 429

BLAST of Cucsa.341380 vs. TAIR10
Match: AT5G56650.1 (AT5G56650.1 IAA-leucine resistant (ILR)-like 1)

HSP 1 Score: 521.2 bits (1341), Expect = 6.5e-148
Identity = 256/418 (61.24%), Postives = 321/418 (76.79%), Query Frame = 1

Query: 32  LSNNPQEIPKKFLQFAKESELFDWMVGIRRKIHENPELGFEEFETSKLIRTELDKLGISY 91
           ++ +   IP  FL+ AK  E+FD MV IRRKIHENPELG+EEFETSK IR+ELD +G+ Y
Sbjct: 26  VAGDVSRIPINFLELAKSPEVFDSMVRIRRKIHENPELGYEEFETSKFIRSELDLIGVKY 85

Query: 92  KYPVASTGVIGFVGSGQPPFVAIRADMDALPMQELVEWEHKSKVPGKMHACGHDAHVAMV 151
           ++PVA TG+IG++G+G+PPFVA+RADMDALP+QE VEWEHKSK PGKMHACGHD HVAM+
Sbjct: 86  RFPVAITGIIGYIGTGEPPFVALRADMDALPIQEAVEWEHKSKNPGKMHACGHDGHVAML 145

Query: 152 LGAAKILQKHSEELKGTVVLVFQPAEEGGGGAMKIIEAGVLDNVNAIFGLHIVHNIPIGK 211
           LGAAKILQ+H + L+GTVVL+FQPAEEG  GA  + E G L NV AIFG+H+    P GK
Sbjct: 146 LGAAKILQQHRQHLQGTVVLIFQPAEEGLSGAKMMREEGALKNVEAIFGIHLSPRTPFGK 205

Query: 212 VAGRSGPLLAGSAFFEAVISGKGGHAAIPQHSIDPILAASNVIVSLQHLVSREADPLDSQ 271
            A  +G  +AG+  FEAVI+GKGGHAAIPQH+IDP++AAS++++SLQHLVSRE DP DS+
Sbjct: 206 AASLAGSFMAGAGAFEAVITGKGGHAAIPQHTIDPVVAASSIVLSLQHLVSRETDPSDSK 265

Query: 272 VVTVAKFQGGGAFNVIPDSVTIGGTFRAFLKDSMVQLKQRIKEVITGQAIVHRCNATVDF 331
           VVTV K  GG AFNVIPDS+TIGGT RAF      QL++RIKE+IT QA VHRCNA+V+ 
Sbjct: 266 VVTVTKVNGGNAFNVIPDSITIGGTLRAF--TGFTQLQERIKEIITKQAAVHRCNASVNL 325

Query: 332 LENDKPIFPPTVNDKELHEHFQNVAGDMLGIDKVKDMQPVMGSEDFSFYQEMIPGYFFFL 391
             N     PPTVN+ +L++ F+ V  D+LG +   +  P MGSEDFS++ E IPG+F  L
Sbjct: 326 APNGNQPMPPTVNNMDLYKKFKKVVRDLLGQEAFVEAVPEMGSEDFSYFAETIPGHFSLL 385

Query: 392 GMENETSGHLDSVHSPYFRINEDALPYGAALHASLATRYLLELQSKVNSPEEKRHDEL 450
           GM++ET G+  S HSP++RINED LPYGAA+HA++A +YL +  SK        HDEL
Sbjct: 386 GMQDETQGYASS-HSPHYRINEDVLPYGAAIHATMAVQYLKDKASK--GSVSGFHDEL 438

BLAST of Cucsa.341380 vs. TAIR10
Match: AT1G44350.1 (AT1G44350.1 IAA-leucine resistant (ILR)-like gene 6)

HSP 1 Score: 426.4 bits (1095), Expect = 2.2e-119
Identity = 212/395 (53.67%), Postives = 275/395 (69.62%), Query Frame = 1

Query: 42  KFLQFAKESELFDWMVGIRRKIHENPELGFEEFETSKLIRTELDKLGISYKYPVASTGVI 101
           + L+   + +   W+  +RR IHENPEL FEE+ETS+LIR+ELD++GI Y+YP+A TG+ 
Sbjct: 75  EILRLTYQPDNVAWLKRVRRTIHENPELAFEEYETSRLIRSELDRMGIMYRYPLAKTGIR 134

Query: 102 GFVGSGQPPFVAIRADMDALPMQELVEWEHKSKVPGKMHACGHDAHVAMVLGAAKILQKH 161
            ++GSG PPFVA+RADMDALP+QE VEWEH SKV GKMHACGHDAHV M+LGAA IL+  
Sbjct: 135 AWIGSGGPPFVAVRADMDALPIQEAVEWEHISKVAGKMHACGHDAHVTMLLGAAHILKAR 194

Query: 162 SEELKGTVVLVFQPAEEGGGGAMKIIEAGVLDNVNAIFGLHIVHNIPIGKVAGRSGPLLA 221
              LKGTVVL+FQPAEE G GA  +IE G LD+V AIF +H+ H  P G +  RSGPLLA
Sbjct: 195 EHLLKGTVVLLFQPAEEAGNGAKNMIEDGALDDVEAIFAVHVSHIHPTGVIGSRSGPLLA 254

Query: 222 GSAFFEAVISGKGGHAAIPQHSIDPILAASNVIVSLQHLVSREADPLDSQVVTVAKFQGG 281
           G   F AVI+ +    A      + +LAAS+ ++SLQ +VSREA PLDSQVV+V  F GG
Sbjct: 255 GCGIFRAVITSEDSRGA-----ANLLLAASSAVISLQGIVSREASPLDSQVVSVTSFDGG 314

Query: 282 GAFNVIPDSVTIGGTFRAFLKDSMVQLKQRIKEVITGQAIVHRCNATVDFLENDKPIFPP 341
            + +V PD+V +GGTFRAF   S   LK+RI+EV+  Q  V  C ATV+F E    I+PP
Sbjct: 315 HSLDVAPDTVVLGGTFRAFSNSSFYYLKKRIQEVLMDQVGVFGCQATVNFFEKQNAIYPP 374

Query: 342 TVNDKELHEHFQNVAGDMLGIDKVKDMQPVMGSEDFSFYQEMIPGYFFFLGMENETSGHL 401
           T N+   + H + V  D+LG         +MG+EDF+FY E+IP  F+F+G+ NE  G +
Sbjct: 375 TTNNDATYNHLKKVTIDLLGDSHFTLAPQMMGAEDFAFYSEIIPAAFYFIGIRNEELGSV 434

Query: 402 DSVHSPYFRINEDALPYGAALHASLATRYLLELQS 437
              HSP+F I+ED+LP GAA+HA++A RYL +  S
Sbjct: 435 HIAHSPHFMIDEDSLPVGAAVHAAVAERYLNDKHS 464

BLAST of Cucsa.341380 vs. NCBI nr
Match: gi|449458197|ref|XP_004146834.1| (PREDICTED: IAA-amino acid hydrolase ILR1-like 4 [Cucumis sativus])

HSP 1 Score: 899.4 bits (2323), Expect = 2.5e-258
Identity = 449/449 (100.00%), Postives = 449/449 (100.00%), Query Frame = 1

Query: 1   MHLIMASLRWISWILIAHLFVSTFVHSDSSSLSNNPQEIPKKFLQFAKESELFDWMVGIR 60
           MHLIMASLRWISWILIAHLFVSTFVHSDSSSLSNNPQEIPKKFLQFAKESELFDWMVGIR
Sbjct: 1   MHLIMASLRWISWILIAHLFVSTFVHSDSSSLSNNPQEIPKKFLQFAKESELFDWMVGIR 60

Query: 61  RKIHENPELGFEEFETSKLIRTELDKLGISYKYPVASTGVIGFVGSGQPPFVAIRADMDA 120
           RKIHENPELGFEEFETSKLIRTELDKLGISYKYPVASTGVIGFVGSGQPPFVAIRADMDA
Sbjct: 61  RKIHENPELGFEEFETSKLIRTELDKLGISYKYPVASTGVIGFVGSGQPPFVAIRADMDA 120

Query: 121 LPMQELVEWEHKSKVPGKMHACGHDAHVAMVLGAAKILQKHSEELKGTVVLVFQPAEEGG 180
           LPMQELVEWEHKSKVPGKMHACGHDAHVAMVLGAAKILQKHSEELKGTVVLVFQPAEEGG
Sbjct: 121 LPMQELVEWEHKSKVPGKMHACGHDAHVAMVLGAAKILQKHSEELKGTVVLVFQPAEEGG 180

Query: 181 GGAMKIIEAGVLDNVNAIFGLHIVHNIPIGKVAGRSGPLLAGSAFFEAVISGKGGHAAIP 240
           GGAMKIIEAGVLDNVNAIFGLHIVHNIPIGKVAGRSGPLLAGSAFFEAVISGKGGHAAIP
Sbjct: 181 GGAMKIIEAGVLDNVNAIFGLHIVHNIPIGKVAGRSGPLLAGSAFFEAVISGKGGHAAIP 240

Query: 241 QHSIDPILAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAF 300
           QHSIDPILAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAF
Sbjct: 241 QHSIDPILAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAF 300

Query: 301 LKDSMVQLKQRIKEVITGQAIVHRCNATVDFLENDKPIFPPTVNDKELHEHFQNVAGDML 360
           LKDSMVQLKQRIKEVITGQAIVHRCNATVDFLENDKPIFPPTVNDKELHEHFQNVAGDML
Sbjct: 301 LKDSMVQLKQRIKEVITGQAIVHRCNATVDFLENDKPIFPPTVNDKELHEHFQNVAGDML 360

Query: 361 GIDKVKDMQPVMGSEDFSFYQEMIPGYFFFLGMENETSGHLDSVHSPYFRINEDALPYGA 420
           GIDKVKDMQPVMGSEDFSFYQEMIPGYFFFLGMENETSGHLDSVHSPYFRINEDALPYGA
Sbjct: 361 GIDKVKDMQPVMGSEDFSFYQEMIPGYFFFLGMENETSGHLDSVHSPYFRINEDALPYGA 420

Query: 421 ALHASLATRYLLELQSKVNSPEEKRHDEL 450
           ALHASLATRYLLELQSKVNSPEEKRHDEL
Sbjct: 421 ALHASLATRYLLELQSKVNSPEEKRHDEL 449

BLAST of Cucsa.341380 vs. NCBI nr
Match: gi|659093536|ref|XP_008447583.1| (PREDICTED: IAA-amino acid hydrolase ILR1-like 4 [Cucumis melo])

HSP 1 Score: 885.6 bits (2287), Expect = 3.7e-254
Identity = 440/449 (98.00%), Postives = 445/449 (99.11%), Query Frame = 1

Query: 1   MHLIMASLRWISWILIAHLFVSTFVHSDSSSLSNNPQEIPKKFLQFAKESELFDWMVGIR 60
           MHLIMASLRW SWILIAHLFVSTFVHSDSSS SNNPQEIPK+FLQFAKESELFDWMVGIR
Sbjct: 1   MHLIMASLRWFSWILIAHLFVSTFVHSDSSSHSNNPQEIPKRFLQFAKESELFDWMVGIR 60

Query: 61  RKIHENPELGFEEFETSKLIRTELDKLGISYKYPVASTGVIGFVGSGQPPFVAIRADMDA 120
           RKIHENPELGFEEFETSKLIRTELDKLGISYKYPVASTGVIGFVGSGQPPFVAIRADMDA
Sbjct: 61  RKIHENPELGFEEFETSKLIRTELDKLGISYKYPVASTGVIGFVGSGQPPFVAIRADMDA 120

Query: 121 LPMQELVEWEHKSKVPGKMHACGHDAHVAMVLGAAKILQKHSEELKGTVVLVFQPAEEGG 180
           LPMQELVEWEHKSKVPGKMHACGHDAHVAMVLGAAKILQKHSEELKGTVVLVFQPAEEGG
Sbjct: 121 LPMQELVEWEHKSKVPGKMHACGHDAHVAMVLGAAKILQKHSEELKGTVVLVFQPAEEGG 180

Query: 181 GGAMKIIEAGVLDNVNAIFGLHIVHNIPIGKVAGRSGPLLAGSAFFEAVISGKGGHAAIP 240
           GGAMKIIEAGVLDNVNAIFGLHIVHNIP+GKVAGRSGP+LAGS FFEAVISGKGGHAAIP
Sbjct: 181 GGAMKIIEAGVLDNVNAIFGLHIVHNIPLGKVAGRSGPMLAGSGFFEAVISGKGGHAAIP 240

Query: 241 QHSIDPILAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAF 300
           QHSIDPILAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAF
Sbjct: 241 QHSIDPILAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAF 300

Query: 301 LKDSMVQLKQRIKEVITGQAIVHRCNATVDFLENDKPIFPPTVNDKELHEHFQNVAGDML 360
           LKDSMVQLKQRIKEVITGQAIVHRCNATVDFLENDKPIFPPTVNDKELHEHFQNVAGDML
Sbjct: 301 LKDSMVQLKQRIKEVITGQAIVHRCNATVDFLENDKPIFPPTVNDKELHEHFQNVAGDML 360

Query: 361 GIDKVKDMQPVMGSEDFSFYQEMIPGYFFFLGMENETSGHLDSVHSPYFRINEDALPYGA 420
           GIDKVK+MQPVMGSEDFSFYQEMIPGYFFFLGMENETSGHLDSVHSPYFRINEDALPYGA
Sbjct: 361 GIDKVKEMQPVMGSEDFSFYQEMIPGYFFFLGMENETSGHLDSVHSPYFRINEDALPYGA 420

Query: 421 ALHASLATRYLLELQSKVNSPEEKRHDEL 450
           ALHASLATRYLLELQ+KVNSPEEKRH EL
Sbjct: 421 ALHASLATRYLLELQTKVNSPEEKRHGEL 449

BLAST of Cucsa.341380 vs. NCBI nr
Match: gi|590619938|ref|XP_007024408.1| (Peptidase M20/M25/M40 family protein [Theobroma cacao])

HSP 1 Score: 695.3 bits (1793), Expect = 7.1e-197
Identity = 349/448 (77.90%), Postives = 393/448 (87.72%), Query Frame = 1

Query: 5   MASLRWISWILIAHLFVSTFVHSDSS-SLSNNPQEIPKKFLQFAKESELFDWMVGIRRKI 64
           M+  +++S++L+ HL  +T V S SS S SN   EIPKKFL FAK  EL DW+VGIRRKI
Sbjct: 1   MSFSKFVSFVLMLHLLNTTVVLSSSSLSSSNGWSEIPKKFLHFAKGQELVDWIVGIRRKI 60

Query: 65  HENPELGFEEFETSKLIRTELDKLGISYKYPVASTGVIGFVGSGQPPFVAIRADMDALPM 124
           HENPELG+EEFETSKLIR ELDK+GI YKYPV+ TGV+G+VG+G+PPFVAIRADMDAL M
Sbjct: 61  HENPELGYEEFETSKLIREELDKMGIPYKYPVSVTGVVGYVGTGKPPFVAIRADMDALAM 120

Query: 125 QELVEWEHKSKVPGKMHACGHDAHVAMVLGAAKILQKHSEELKGTVVLVFQPAEEGGGGA 184
           +ELVE+EHKSK PGKMHACGHDAHV+M+LGAAKIL++H EELKGTV+LVFQPAEEGGGGA
Sbjct: 121 EELVEFEHKSKNPGKMHACGHDAHVSMLLGAAKILKEHLEELKGTVLLVFQPAEEGGGGA 180

Query: 185 MKIIEAGVLDNVNAIFGLHIVHNIPIGKVAGRSGPLLAGSAFFEAVISGKGGHAAIPQHS 244
            K+++AGVL NV+AIFGLH+V + PIG VA R GPLLAGS FFEAVISGKGGHAAIPQHS
Sbjct: 181 KKMLDAGVLKNVDAIFGLHVVPDQPIGTVASRPGPLLAGSGFFEAVISGKGGHAAIPQHS 240

Query: 245 IDPILAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFLKD 304
           IDPILAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAF K+
Sbjct: 241 IDPILAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFSKE 300

Query: 305 SMVQLKQRIKEVITGQAIVHRCNATVDFLENDK--PIFPPTVNDKELHEHFQNVAGDMLG 364
           S+ QLKQRI+EVI GQA V RC+ATVDF EN+K  PIFPPTVN+K+LHEHFQNVAGDMLG
Sbjct: 301 SLTQLKQRIEEVIKGQAAVQRCSATVDFYENEKPVPIFPPTVNNKDLHEHFQNVAGDMLG 360

Query: 365 IDKVKDMQPVMGSEDFSFYQEMIPGYFFFLGMENETSGHLDSVHSPYFRINEDALPYGAA 424
            DKVKDMQP+MGSEDF+FYQE IPGYFF LGM ++T   L SVHSPYF INED LPYGAA
Sbjct: 361 ADKVKDMQPLMGSEDFAFYQEAIPGYFFMLGMRDDTGPQLKSVHSPYFMINEDVLPYGAA 420

Query: 425 LHASLATRYLLELQSKVNSPEEKRHDEL 450
           LHASLA RYLL  + K +SP+E+ HDEL
Sbjct: 421 LHASLAARYLLAAEPKFHSPKEELHDEL 448

BLAST of Cucsa.341380 vs. NCBI nr
Match: gi|225442363|ref|XP_002281321.1| (PREDICTED: IAA-amino acid hydrolase ILR1-like 4 [Vitis vinifera])

HSP 1 Score: 694.1 bits (1790), Expect = 1.6e-196
Identity = 340/445 (76.40%), Postives = 388/445 (87.19%), Query Frame = 1

Query: 5   MASLRWISWILIAHLFVSTFVHSDSSSLSNNPQEIPKKFLQFAKESELFDWMVGIRRKIH 64
           M    W+SWI I  LF  T + S+SS  SN    IP  FL FA++ E+ DW+VG+RRKIH
Sbjct: 1   MTYTEWVSWIFILCLFGPTPISSESSLSSN----IPTNFLSFARKQEVVDWLVGVRRKIH 60

Query: 65  ENPELGFEEFETSKLIRTELDKLGISYKYPVASTGVIGFVGSGQPPFVAIRADMDALPMQ 124
           ENPELGFEE ETSKL+R ELDK+GI YKYPVA TGV+GFVG+G+PPFVAIRADMDAL MQ
Sbjct: 61  ENPELGFEEVETSKLVRAELDKMGIPYKYPVAVTGVLGFVGTGEPPFVAIRADMDALAMQ 120

Query: 125 ELVEWEHKSKVPGKMHACGHDAHVAMVLGAAKILQKHSEELKGTVVLVFQPAEEGGGGAM 184
           E+VEWEHKSK+PGKMHACGHD+HVAM+LGAAKILQ+H EEL+GTV+LVFQPAEEGGGGA 
Sbjct: 121 EMVEWEHKSKIPGKMHACGHDSHVAMLLGAAKILQEHREELQGTVILVFQPAEEGGGGAK 180

Query: 185 KIIEAGVLDNVNAIFGLHIVHNIPIGKVAGRSGPLLAGSAFFEAVISGKGGHAAIPQHSI 244
           KI++AGVL+NVNAIFGLH+  ++PIG+VA RSGPLLAGS FFEAVISGKGGHAAIPQHSI
Sbjct: 181 KILDAGVLENVNAIFGLHVSPDLPIGEVASRSGPLLAGSGFFEAVISGKGGHAAIPQHSI 240

Query: 245 DPILAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFLKDS 304
           DPILAASNVIVSLQHLVSREADPL+SQVVTVAKFQGGGAFNVIPDSVTIGGTFRAF K+S
Sbjct: 241 DPILAASNVIVSLQHLVSREADPLESQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFSKES 300

Query: 305 MVQLKQRIKEVITGQAIVHRCNATVDFLENDKPIFPPTVNDKELHEHFQNVAGDMLGIDK 364
           ++QLKQRI+EVIT QA V RCNATVDF E +KP+FP T+N+  LH+HFQNV G+MLG+  
Sbjct: 301 IMQLKQRIEEVITRQAAVQRCNATVDFHEKEKPLFPATINNPNLHKHFQNVVGNMLGVHN 360

Query: 365 VKDMQPVMGSEDFSFYQEMIPGYFFFLGMENETSGHLDSVHSPYFRINEDALPYGAALHA 424
           VKDMQP+MGSEDFSFYQE +PGYFFFLGM++E  G L SVHSP+F+INEDALPYGAALHA
Sbjct: 361 VKDMQPLMGSEDFSFYQEEMPGYFFFLGMKDEALGRLPSVHSPHFKINEDALPYGAALHA 420

Query: 425 SLATRYLLELQSKVNSPEEKRHDEL 450
           SLA  YLLE+Q + +S E K HDEL
Sbjct: 421 SLAATYLLEIQPQPSSREGKHHDEL 441

BLAST of Cucsa.341380 vs. NCBI nr
Match: gi|224070738|ref|XP_002303219.1| (putative auxin-amidohydrolase precursor family protein [Populus trichocarpa])

HSP 1 Score: 693.3 bits (1788), Expect = 2.7e-196
Identity = 337/446 (75.56%), Postives = 385/446 (86.32%), Query Frame = 1

Query: 5   MASLRWISWILIAHLFVSTFVHSDSSSLS-NNPQEIPKKFLQFAKESELFDWMVGIRRKI 64
           M+   W+S        +  F++  S  LS N   +IP +FL +AK+ ELFDWMVG+RRKI
Sbjct: 1   MSLFNWVS--------LGFFLYLLSPILSLNGSSDIPSRFLNYAKKEELFDWMVGVRRKI 60

Query: 65  HENPELGFEEFETSKLIRTELDKLGISYKYPVASTGVIGFVGSGQPPFVAIRADMDALPM 124
           HENPELGFEEFETSKL+R ELDK+G+ YK+P++ TGV+GF+GSG+PPFVA+RADMDAL M
Sbjct: 61  HENPELGFEEFETSKLVRAELDKIGVKYKHPLSVTGVVGFIGSGKPPFVALRADMDALAM 120

Query: 125 QELVEWEHKSKVPGKMHACGHDAHVAMVLGAAKILQKHSEELKGTVVLVFQPAEEGGGGA 184
           QE+VEWE+KSKVPGKMHACGHD+HVAM+LGAAKILQ H EELKGTVVL+FQPAEEGGGGA
Sbjct: 121 QEMVEWEYKSKVPGKMHACGHDSHVAMLLGAAKILQDHREELKGTVVLIFQPAEEGGGGA 180

Query: 185 MKIIEAGVLDNVNAIFGLHIVHNIPIGKVAGRSGPLLAGSAFFEAVISGKGGHAAIPQHS 244
            K+I+ G L+NVNAIFGLH+ + +PIG+VA R GPLLAGS FFEAVISGKGGHAAIPQHS
Sbjct: 181 KKMIDEGALENVNAIFGLHVANKLPIGEVASRHGPLLAGSGFFEAVISGKGGHAAIPQHS 240

Query: 245 IDPILAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFLKD 304
           IDPILAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFLK+
Sbjct: 241 IDPILAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFLKE 300

Query: 305 SMVQLKQRIKEVITGQAIVHRCNATVDFLENDKPIFPPTVNDKELHEHFQNVAGDMLGID 364
           S +QLKQRI+EV+TGQA V RC A ++FLEN+KP FPPT+NDK LH++F+ VA DMLGID
Sbjct: 301 SFMQLKQRIEEVVTGQAAVQRCKAVINFLENEKPFFPPTINDKYLHDYFRIVASDMLGID 360

Query: 365 KVKDMQPVMGSEDFSFYQEMIPGYFFFLGMENETSGHLDSVHSPYFRINEDALPYGAALH 424
           KVKDMQP+MGSEDF+FYQEMIPGYFFF+GM+NET   L S HSPYF INED LPYGAALH
Sbjct: 361 KVKDMQPLMGSEDFAFYQEMIPGYFFFIGMQNETHKQLQSPHSPYFEINEDVLPYGAALH 420

Query: 425 ASLATRYLLELQSKVNSPEEKRHDEL 450
           ASLA RYLLE Q +V  PEE  HDEL
Sbjct: 421 ASLAARYLLEFQPEVTLPEENDHDEL 438

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
ILL4_ARATH2.4e-18469.66IAA-amino acid hydrolase ILR1-like 4 OS=Arabidopsis thaliana GN=ILL4 PE=1 SV=2[more]
ILL1_ORYSI1.0e-16670.80IAA-amino acid hydrolase ILR1-like 1 OS=Oryza sativa subsp. indica GN=ILL1 PE=2 ... [more]
ILL5_ARATH1.3e-16665.75IAA-amino acid hydrolase ILR1-like 5 OS=Arabidopsis thaliana GN=ILL5 PE=3 SV=1[more]
ILL1_ORYSJ3.8e-16670.56IAA-amino acid hydrolase ILR1-like 1 OS=Oryza sativa subsp. japonica GN=ILL1 PE=... [more]
ILL2_ARATH1.4e-15263.92IAA-amino acid hydrolase ILR1-like 2 OS=Arabidopsis thaliana GN=ILL2 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0LFU9_CUCSA1.7e-258100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_3G850490 PE=4 SV=1[more]
A0A061GAV6_THECC5.0e-19777.90Peptidase M20/M25/M40 family protein OS=Theobroma cacao GN=TCM_028981 PE=4 SV=1[more]
D7U044_VITVI1.1e-19676.40Putative uncharacterized protein OS=Vitis vinifera GN=VIT_09s0002g02630 PE=4 SV=... [more]
B9GVN2_POPTR1.9e-19675.56Putative auxin-amidohydrolase family protein OS=Populus trichocarpa GN=POPTR_000... [more]
A5BUS2_VITVI2.5e-19676.40Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_033718 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G51760.11.3e-18569.66 peptidase M20/M25/M40 family protein[more]
AT1G51780.17.4e-16865.75 IAA-leucine resistant (ILR)-like gene 5[more]
AT5G56660.17.9e-15463.92 IAA-leucine resistant (ILR)-like 2[more]
AT5G56650.16.5e-14861.24 IAA-leucine resistant (ILR)-like 1[more]
AT1G44350.12.2e-11953.67 IAA-leucine resistant (ILR)-like gene 6[more]
Match NameE-valueIdentityDescription
gi|449458197|ref|XP_004146834.1|2.5e-258100.00PREDICTED: IAA-amino acid hydrolase ILR1-like 4 [Cucumis sativus][more]
gi|659093536|ref|XP_008447583.1|3.7e-25498.00PREDICTED: IAA-amino acid hydrolase ILR1-like 4 [Cucumis melo][more]
gi|590619938|ref|XP_007024408.1|7.1e-19777.90Peptidase M20/M25/M40 family protein [Theobroma cacao][more]
gi|225442363|ref|XP_002281321.1|1.6e-19676.40PREDICTED: IAA-amino acid hydrolase ILR1-like 4 [Vitis vinifera][more]
gi|224070738|ref|XP_002303219.1|2.7e-19675.56putative auxin-amidohydrolase precursor family protein [Populus trichocarpa][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR002933Peptidase_M20
IPR011650Peptidase_M20_dimer
IPR017439Amidohydrolase
Vocabulary: Biological Process
TermDefinition
GO:0008152metabolic process
Vocabulary: Molecular Function
TermDefinition
GO:0016787hydrolase activity
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006558 L-phenylalanine metabolic process
biological_process GO:0008152 metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0047980 hippurate hydrolase activity
molecular_function GO:0016787 hydrolase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.341380.1Cucsa.341380.1mRNA


Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002933Peptidase M20PFAMPF01546Peptidase_M20coord: 113..427
score: 3.5
IPR011650Peptidase M20, dimerisation domainGENE3DG3DSA:3.30.70.360coord: 221..341
score: 1.7
IPR011650Peptidase M20, dimerisation domainPFAMPF07687M20_dimercoord: 222..317
score: 5.9
IPR011650Peptidase M20, dimerisation domainunknownSSF55031Bacterial exopeptidase dimerisation domaincoord: 222..331
score: 1.96
IPR017439AmidohydrolasePIRPIRSF005962Amidohydrol_AmhXcoord: 41..435
score: 5.2E
IPR017439AmidohydrolaseTIGRFAMsTIGR01891TIGR01891coord: 56..418
score: 1.9E
NoneNo IPR availableGENE3DG3DSA:3.40.630.10coord: 342..432
score: 1.0E-108coord: 50..218
score: 1.0E
NoneNo IPR availablePANTHERPTHR11014PEPTIDASE M20 FAMILY MEMBERcoord: 1..435
score: 1.8E
NoneNo IPR availablePANTHERPTHR11014:SF55IAA-AMINO ACID HYDROLASE ILR1-LIKE 1-RELATEDcoord: 1..435
score: 1.8E
NoneNo IPR availableunknownSSF53187Zn-dependent exopeptidasescoord: 342..431
score: 2.68E-90coord: 50..237
score: 2.68

The following gene(s) are paralogous to this gene:

None