Cucsa.337190 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.337190 vs. Swiss-Prot
Match: RGA4_SOLBU (Putative disease resistance protein RGA4 OS=Solanum bulbocastanum GN=RGA4 PE=2 SV=1)

HSP 1 Score: 239.6 bits (610), Expect = 1.2e-61
Identity = 188/665 (28.27%), Postives = 312/665 (46.92%), Query Frame = 1

Query: 32  SLKSILMDAEEKQEQSRGLQNWLEELQNVFSQIEGFIDEHKEEAYE------GIGKQVLA 91
           +++++L DA+EKQ + + ++NWL++L +   +++  + E K EA        G     + 
Sbjct: 40  TIQAVLQDAQEKQLKDKAIENWLQKLNSAAYEVDDILGECKNEAIRFEQSRLGFYHPGII 99

Query: 92  PFSCSSNQIARTWKMEKLFDHLNEVAAKMYEFNLTERHT---GAIKTETTNSFLTATEVS 151
            F    ++I R  +M+++ + L+ ++ +  +F+  E+ T    A  T  T   LT  +V 
Sbjct: 100 NFR---HKIGR--RMKEIMEKLDAISEERRKFHFLEKITERQAAAATRETGFVLTEPKVY 159

Query: 152 TRLMKPSWKVLYPLTNAPKFYQDERYRKILNDFK-NPTLGFFHIVGEAGIGKSTLAKFIY 211
            R  +                +DE  + ++N+      L  F I+G  G+GK+TLA+ I+
Sbjct: 160 GRDKE----------------EDEIVKILINNVNVAEELPVFPIIGMGGLGKTTLAQMIF 219

Query: 212 NDPEVEGMFPSRLWVCVKEEFDTQRLMKEIL-NFSYSPATCDNLTTKLCPTDQYLRERTF 271
           ND  V   F  ++WVCV ++FD +RL+K I+ N   S    ++L +      + L  + +
Sbjct: 220 NDERVTKHFNPKIWVCVSDDFDEKRLIKTIIGNIERSSPHVEDLASFQKKLQELLNGKRY 279

Query: 272 LLVFQDLSIKNLDNCSLFTSLLMMGKPGSKIIVTTQNEEIANAIELTMIYKVGQQSEQNR 331
           LLV  D                              N+++    +L  +  VG +     
Sbjct: 280 LLVLDD----------------------------VWNDDLEKWAKLRAVLTVGARGASIL 339

Query: 332 SQTALDTVTKETANVNNADQFVQANPLGKIDQSIPSQTIFKVKRLSEKDSLSLF--KDYA 391
           + T L+                      K+   + +   + +  LS  DSL LF  + + 
Sbjct: 340 ATTRLE----------------------KVGSIMGTLQPYHLSNLSPHDSLLLFMQRAFG 399

Query: 392 STYEGNEKDIM---KTLKKCNGIPLAIKCLGSMLSLGPPATKW--MEDNERQKGDNESSS 451
              E N   +    + +KKC G+PLA K LG +L      ++W  + DNE      + SS
Sbjct: 400 QQKEANPNLVAIGKEIVKKCGGVPLAAKTLGGLLRFKREESEWEHVRDNEIWSLPQDESS 459

Query: 452 TFSILKLCYNEMPSHLKRCFLYCSQLPNDSILSSNDVIQLWMANGLLRSRQENYLSLEDI 511
               L+L Y+ +P  L++CF YC+  P D+ +   ++I LWMA+G L S+    L LED+
Sbjct: 460 ILPALRLSYHHLPLDLRQCFAYCAVFPKDTKMIKENLITLWMAHGFLLSK--GNLELEDV 519

Query: 512 GEIYFKELCSRCFLQDVEEYGLGYWFKMHPLIRELARLVQKRTKDL-----ISIKPVTNV 571
           G   + EL  R F Q++E      +FK+H LI +LA  +   +        I++K   + 
Sbjct: 520 GNEVWNELYLRSFFQEIEAKSGNTYFKIHDLIHDLATSLFSASASCGNIREINVKDYKHT 579

Query: 572 TSIAFPVRDEVPSSSFLAEKCISKFQHLRLLYLGHTDLQEIPNTIETLNHLTYLDLQGNK 631
            SI F       S S L      KF  LR+L L ++ L+++P++I  L HL YLDL  N 
Sbjct: 580 VSIGFAAVVSSYSPSLL-----KKFVSLRVLNLSYSKLEQLPSSIGDLLHLRYLDLSCN- 625

Query: 632 NIKRLPNAICNLQHLQTLILASCSALEELPKDICKLSNLRYLWVTSNKLRLHKNGVGTMT 674
           N + LP  +C LQ+LQTL + +C +L  LPK   KLS+LR+L V    L      +G +T
Sbjct: 640 NFRSLPERLCKLQNLQTLDVHNCYSLNCLPKQTSKLSSLRHLVVDGCPLTSTPPRIGLLT 625

HSP 2 Score: 50.4 bits (119), Expect = 1.0e-04
Identity = 30/89 (33.71%), Postives = 50/89 (56.18%), Query Frame = 1

Query: 638 PKDICKLSNLRYLWVTSNKLRLHKNGVGTMTSLRFLAIGGCDKLQDLFERPSCLVRLETL 697
           P  + K  +LR L ++ +KL    + +G +  LR+L +  C+  + L ER   L  L+TL
Sbjct: 520 PSLLKKFVSLRVLNLSYSKLEQLPSSIGDLLHLRYLDLS-CNNFRSLPERLCKLQNLQTL 579

Query: 698 MIYDCNSLQLLPNEMGSLISLQNLVIWSC 727
            +++C SL  LP +   L SL++LV+  C
Sbjct: 580 DVHNCYSLNCLPKQTSKLSSLRHLVVDGC 607

HSP 3 Score: 44.3 bits (103), Expect = 7.3e-03
Identity = 28/90 (31.11%), Postives = 51/90 (56.67%), Query Frame = 1

Query: 595 TLNHLTYLDLQGNKNIKRLPNAICNLQHLQTLILASCSALEELPKD-ICKLSNLRYLWVT 654
           +L +L +L     KN+K LP ++ +L  L+ L + SC +LE  P+  +  L++L  L+V 
Sbjct: 876 SLTNLEFLSFFDFKNLKDLPTSLTSLNALKRLQIESCDSLESFPEQGLEGLTSLTQLFVK 935

Query: 655 SNK-LRLHKNGVGTMTSLRFLAIGGCDKLQ 683
             K L+    G+  +T+L  L + GC +++
Sbjct: 936 YCKMLKCLPEGLQHLTALTNLGVSGCPEVE 965

BLAST of Cucsa.337190 vs. Swiss-Prot
Match: RGA1_SOLBU (Putative disease resistance protein RGA1 OS=Solanum bulbocastanum GN=RGA1 PE=2 SV=2)

HSP 1 Score: 229.9 bits (585), Expect = 9.4e-59
Identity = 184/674 (27.30%), Postives = 311/674 (46.14%), Query Frame = 1

Query: 24  RRIEYTMLSLKSILMDAEEKQEQSRGLQNWLEELQNVFSQIEGFIDEHKEEAYEGIGKQV 83
           +R+     +++++L DA+EKQ   + L+NWL++L     +++  +DE+K +A   +  + 
Sbjct: 32  QRLSSMFSTIQAVLEDAQEKQLNDKPLENWLQKLNAATYEVDDILDEYKTKATRFLQSEY 91

Query: 84  ------LAPFSCSSNQIARTWKMEKLFDHLNEVAAKMYEFNLTERHTGA-IKTETTNSFL 143
                 + PF     +     +M+++   LN +A +  +F+L E+       T  T S L
Sbjct: 92  GRYHPKVIPFRHKVGK-----RMDQVMKKLNAIAEERKKFHLQEKIIERQAATRETGSVL 151

Query: 144 TATEVSTRLMKPSWKVLYPLTNAPKFYQDERYRKILNDFKNPT-LGFFHIVGEAGIGKST 203
           T  +V  R  +                +DE  + ++N   +   L    I+G  G+GK+T
Sbjct: 152 TEPQVYGRDKE----------------KDEIVKILINTASDAQKLSVLPILGMGGLGKTT 211

Query: 204 LAKFIYNDPEVEGMFPSRLWVCVKEEFDTQRLMKEILNFSYSPATCDNLTTKLCPTDQYL 263
           L++ ++ND  V   F  ++W+C+ ++F+ +RL+K I+      +  D     L P  + L
Sbjct: 212 LSQMVFNDQRVTERFYPKIWICISDDFNEKRLIKAIVESIEGKSLSD---MDLAPLQKKL 271

Query: 264 RERTFLLVFQDLSIKNLDNCSLFTSLLMMGKPGSKIIVTTQNEEIANAIELTMIYKVGQQ 323
           +E                        L+ GK    ++    NE+      L  + KVG  
Sbjct: 272 QE------------------------LLNGKRYFLVLDDVWNEDQHKWANLRAVLKVGAS 331

Query: 324 SEQNRSQTALDTVTKETANVNNADQFVQANPLGKIDQSIPSQTIFKVKRLSEKDSLSLFK 383
                + T L+                      K+   + +   +++  LS +D   LF 
Sbjct: 332 GAFVLTTTRLE----------------------KVGSIMGTLQPYELSNLSPEDCWFLFM 391

Query: 384 DYASTYEGNEKDIMKTL--------KKCNGIPLAIKCLGSMLSLGPPATKW--MEDNERQ 443
             A    G++++I   L        KKC G+PLA K LG +L       +W  + D+   
Sbjct: 392 QRAF---GHQEEINPNLMAIGKEIVKKCGGVPLAAKTLGGILRFKREEREWEHVRDSPIW 451

Query: 444 KGDNESSSTFSILKLCYNEMPSHLKRCFLYCSQLPNDSILSSNDVIQLWMANGLLRSRQE 503
               + SS    L+L Y+ +P  L++CF+YC+  P D+ ++  ++I  WMA+G L S+  
Sbjct: 452 NLPQDESSILPALRLSYHHLPLDLRQCFVYCAVFPKDTKMAKENLIAFWMAHGFLLSKGN 511

Query: 504 NYLSLEDIGEIYFKELCSRCFLQDVEEYGLGYWFKMHPLIRELARLVQKRTKDLISIKPV 563
             L LED+G   + EL  R F Q++E      +FKMH LI +LA  +        +I+ +
Sbjct: 512 --LELEDVGNEVWNELYLRSFFQEIEVESGKTYFKMHDLIHDLATSLFSANTSSSNIREI 571

Query: 564 TN-----VTSIAFPVRDEVPSSSFLAEKCISKFQHLRLLYLGHTDLQEIPNTIETLNHLT 623
                  + SI F    EV SS   +   + KF  LR+L L +++L ++P++I  L HL 
Sbjct: 572 NANYDGYMMSIGFA---EVVSS--YSPSLLQKFVSLRVLNLRNSNLNQLPSSIGDLVHLR 625

Query: 624 YLDLQGNKNIKRLPNAICNLQHLQTLILASCSALEELPKDICKLSNLRYLWVTSNKLRLH 675
           YLDL GN  I+ LP  +C LQ+LQTL L  C +L  LPK   KL +LR L +    L   
Sbjct: 632 YLDLSGNFRIRNLPKRLCKLQNLQTLDLHYCDSLSCLPKQTSKLGSLRNLLLDGCSLTST 625

HSP 2 Score: 45.8 bits (107), Expect = 2.5e-03
Identity = 33/109 (30.28%), Postives = 50/109 (45.87%), Query Frame = 1

Query: 594 ETLNHLTYLDLQGNKNIKRLPNAICNLQHLQTLILASCSALEELPKDICKLSNLRYLWVT 653
           ++L +L YL +   +N+K LP ++ +L  L++L    C ALE LP++             
Sbjct: 865 KSLANLKYLKISFFRNLKELPTSLASLNALKSLKFEFCDALESLPEE------------- 924

Query: 654 SNKLRLHKNGVGTMTSLRFLAIGGCDKLQDLFERPSCLVRLETLMIYDC 703
                    GV  +TSL  L++  C  L+ L E    L  L TL I  C
Sbjct: 925 ---------GVKGLTSLTELSVSNCMMLKCLPEGLQHLTALTTLTITQC 951

BLAST of Cucsa.337190 vs. Swiss-Prot
Match: RGA3_SOLBU (Putative disease resistance protein RGA3 OS=Solanum bulbocastanum GN=RGA3 PE=2 SV=2)

HSP 1 Score: 218.0 bits (554), Expect = 3.7e-55
Identity = 181/666 (27.18%), Postives = 306/666 (45.95%), Query Frame = 1

Query: 33  LKSILMDAEEKQEQSRGLQNWLEELQNVFSQIEGFIDEHKEEAYEGIGKQVLAPFSCSSN 92
           ++++L DA+EKQ + + ++NWL++L     +++  +D+ K EA     + VL  +     
Sbjct: 41  IQAVLEDAQEKQLKYKAIKNWLQKLNVAAYEVDDILDDCKTEAAR-FKQAVLGRYH--PR 100

Query: 93  QIARTWK----MEKLFDHLNEVAAKMYEFNLTERHTGAIKTETTNSF-LTATEVSTRLMK 152
            I   +K    M+++ + L+ +A +   F+L ER            F LT  +V  R  +
Sbjct: 101 TITFCYKVGKRMKEMMEKLDAIAEERRNFHLDERIIERQAARRQTGFVLTEPKVYGREKE 160

Query: 153 PSWKVLYPLTNAPKFYQDERYRKILNDFK-NPTLGFFHIVGEAGIGKSTLAKFIYNDPEV 212
                           +DE  + ++N+   +  +    I+G  G+GK+TLA+ ++ND  +
Sbjct: 161 ----------------EDEIVKILINNVSYSEEVPVLPILGMGGLGKTTLAQMVFNDQRI 220

Query: 213 EGMFPSRLWVCVKEEFDTQRLMKEILNFSYSPATCDNLTTKLCPTDQYLRERTFLLVFQD 272
              F  ++WVCV ++FD +RL+K I+      +  D     L P  + L+E         
Sbjct: 221 TEHFNLKIWVCVSDDFDEKRLIKAIVESIEGKSLGD---MDLAPLQKKLQE--------- 280

Query: 273 LSIKNLDNCSLFTSLLMMGKPGSKIIVTTQNEEIANAIELTMIYKVGQQSEQNRSQTALD 332
                          L+ GK    ++    NE+      L  + K+G         T L+
Sbjct: 281 ---------------LLNGKRYFLVLDDVWNEDQEKWDNLRAVLKIGASGASILITTRLE 340

Query: 333 TVTKETANVNNADQFVQANPLGKIDQSIPSQTIFKVKRLSEKDSLSLFKDYASTYEGNEK 392
                                 KI   + +  ++++  LS++D   LFK  A  ++    
Sbjct: 341 ----------------------KIGSIMGTLQLYQLSNLSQEDCWLLFKQRAFCHQTETS 400

Query: 393 DIM-----KTLKKCNGIPLAIKCLGSMLSLGPPATKW--MEDNERQKGDNESSSTFSILK 452
             +     + +KKC G+PLA K LG +L      ++W  + D+E      + +S    L+
Sbjct: 401 PKLMEIGKEIVKKCGGVPLAAKTLGGLLRFKREESEWEHVRDSEIWNLPQDENSVLPALR 460

Query: 453 LCYNEMPSHLKRCFLYCSQLPNDSILSSNDVIQLWMANGLLRSRQENYLSLEDIGEIYFK 512
           L Y+ +P  L++CF YC+  P D+ +    +I LWMA+  L S+    + LED+G   + 
Sbjct: 461 LSYHHLPLDLRQCFAYCAVFPKDTKIEKEYLIALWMAHSFLLSK--GNMELEDVGNEVWN 520

Query: 513 ELCSRCFLQDVEEYGLGYWFKMHPLIRELARLV-----QKRTKDLISIKP-------VTN 572
           EL  R F Q++E      +FKMH LI +LA  +       R+   I++K        VTN
Sbjct: 521 ELYLRSFFQEIEVKSGKTYFKMHDLIHDLATSMFSASASSRSIRQINVKDDEDMMFIVTN 580

Query: 573 VTSIAFPVRDEVPSSSFLAEKCISKFQHLRLLYLGHTDLQEIPNTIETLNHLTYLDLQGN 632
              +      EV SS   +     +F  LR+L L +++ +++P+++  L HL YLDL GN
Sbjct: 581 YKDMMSIGFSEVVSS--YSPSLFKRFVSLRVLNLSNSEFEQLPSSVGDLVHLRYLDLSGN 633

Query: 633 KNIKRLPNAICNLQHLQTLILASCSALEELPKDICKLSNLRYLWVTSNKLRLHKNGVGTM 674
           K I  LP  +C LQ+LQTL L +C +L  LPK   KL +LR L +    L      +G +
Sbjct: 641 K-ICSLPKRLCKLQNLQTLDLYNCQSLSCLPKQTSKLCSLRNLVLDHCPLTSMPPRIGLL 633

HSP 2 Score: 49.7 bits (117), Expect = 1.7e-04
Identity = 29/81 (35.80%), Postives = 48/81 (59.26%), Query Frame = 1

Query: 646 NLRYLWVTSNKLRLHKNGVGTMTSLRFLAIGGCDKLQDLFERPSCLVRLETLMIYDCNSL 705
           +LR L +++++     + VG +  LR+L + G +K+  L +R   L  L+TL +Y+C SL
Sbjct: 536 SLRVLNLSNSEFEQLPSSVGDLVHLRYLDLSG-NKICSLPKRLCKLQNLQTLDLYNCQSL 595

Query: 706 QLLPNEMGSLISLQNLVIWSC 727
             LP +   L SL+NLV+  C
Sbjct: 596 SCLPKQTSKLCSLRNLVLDHC 615

BLAST of Cucsa.337190 vs. Swiss-Prot
Match: RGA2_SOLBU (Disease resistance protein RGA2 OS=Solanum bulbocastanum GN=RGA2 PE=1 SV=1)

HSP 1 Score: 216.9 bits (551), Expect = 8.2e-55
Identity = 186/682 (27.27%), Postives = 313/682 (45.89%), Query Frame = 1

Query: 24  RRIEYTMLSLKSILMDAEEKQEQSRGLQNWLEELQNVFSQIEGFIDEHKEEAYE------ 83
           +R+     +++++L DA+EKQ  ++ L+NWL++L     +++  +DE+K +A        
Sbjct: 32  QRLSSMFSTIQAVLEDAQEKQLNNKPLENWLQKLNAATYEVDDILDEYKTKATRFSQSEY 91

Query: 84  GIGKQVLAPFSCSSNQIARTWKMEKLFDHLNEVAAKMYEFNLTER--HTGAIKTETTNSF 143
           G     + PF     +     +M+++   L  +A +   F+L E+     A++ ET  S 
Sbjct: 92  GRYHPKVIPFRHKVGK-----RMDQVMKKLKAIAEERKNFHLHEKIVERQAVRRET-GSV 151

Query: 144 LTATEVSTRLMKPSWKVLYPLTNAPKFYQDERYRKILNDFKNPT-LGFFHIVGEAGIGKS 203
           LT  +V  R  +                +DE  + ++N+  +   L    I+G  G+GK+
Sbjct: 152 LTEPQVYGRDKE----------------KDEIVKILINNVSDAQHLSVLPILGMGGLGKT 211

Query: 204 TLAKFIYNDPEVEGMFPSRLWVCVKEEFDTQRLMKEILNFSYSPATCDNLTTK------- 263
           TLA+ ++ND  V   F S++W+CV E+FD +RL+K         A  +++  +       
Sbjct: 212 TLAQMVFNDQRVTEHFHSKIWICVSEDFDEKRLIK---------AIVESIEGRPLLGEMD 271

Query: 264 LCPTDQYLRERTFLLVFQDLSIKNLDNCSLFTSLLMMGKPGSKIIVTTQNEEIANAIELT 323
           L P  + L+E                        L+ GK    ++    NE+      L 
Sbjct: 272 LAPLQKKLQE------------------------LLNGKRYLLVLDDVWNEDQQKWANLR 331

Query: 324 MIYKVGQQSEQNRSQTALDTVTKETANVNNADQFVQANPLGKIDQSIPSQTIFKVKRLSE 383
            + KVG       + T L+                      K+   + +   +++  LS+
Sbjct: 332 AVLKVGASGASVLTTTRLE----------------------KVGSIMGTLQPYELSNLSQ 391

Query: 384 KDSLSLFKDYASTYEGNEKDIMKTL--------KKCNGIPLAIKCLGSMLSLGPPATKW- 443
           +D   LF   A    G++++I   L        KK  G+PLA K LG +L        W 
Sbjct: 392 EDCWLLFMQRAF---GHQEEINPNLVAIGKEIVKKSGGVPLAAKTLGGILCFKREERAWE 451

Query: 444 -MEDNERQKGDNESSSTFSILKLCYNEMPSHLKRCFLYCSQLPNDSILSSNDVIQLWMAN 503
            + D+       + SS    L+L Y+++P  LK+CF YC+  P D+ +    +I LWMA+
Sbjct: 452 HVRDSPIWNLPQDESSILPALRLSYHQLPLDLKQCFAYCAVFPKDAKMEKEKLISLWMAH 511

Query: 504 GLLRSRQENYLSLEDIGEIYFKELCSRCFLQDVEEYGLGYWFKMHPLIRELARLVQKRTK 563
           G L S+    + LED+G+  +KEL  R F Q++E      +FKMH LI +LA  +     
Sbjct: 512 GFLLSK--GNMELEDVGDEVWKELYLRSFFQEIEVKDGKTYFKMHDLIHDLATSLFSANT 571

Query: 564 DLISIKPV-----TNVTSIAFPVRDEVPSSSFLAEKCISKFQHLRLLYLGHTDLQEIPNT 623
              +I+ +     T++ SI F          F     + KF  LR+L LG +   ++P++
Sbjct: 572 SSSNIREINKHSYTHMMSIGF-----AEVVFFYTLPPLEKFISLRVLNLGDSTFNKLPSS 625

Query: 624 IETLNHLTYLDLQGNKNIKRLPNAICNLQHLQTLILASCSALEELPKDICKLSNLRYLWV 674
           I  L HL YL+L G+  ++ LP  +C LQ+LQTL L  C+ L  LPK+  KL +LR L +
Sbjct: 632 IGDLVHLRYLNLYGS-GMRSLPKQLCKLQNLQTLDLQYCTKLCCLPKETSKLGSLRNLLL 625

HSP 2 Score: 49.3 bits (116), Expect = 2.3e-04
Identity = 32/90 (35.56%), Postives = 52/90 (57.78%), Query Frame = 1

Query: 594 ETLNHLTYLDLQGNKNIKRLPNAICNLQHLQTLILASCSALEELPKD-ICKLSNLRYLWV 653
           + L +L YL +    N+K LP ++ +L  L++L +  C ALE LP++ +  LS+L  L+V
Sbjct: 856 KNLANLKYLTISRCNNLKELPTSLASLNALKSLKIQLCCALESLPEEGLEGLSSLTELFV 915

Query: 654 T-SNKLRLHKNGVGTMTSLRFLAIGGCDKL 682
              N L+    G+  +T+L  L I GC +L
Sbjct: 916 EHCNMLKCLPEGLQHLTTLTSLKIRGCPQL 945

BLAST of Cucsa.337190 vs. Swiss-Prot
Match: R13L1_ARATH (Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana GN=RPPL1 PE=3 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 1.7e-28
Identity = 105/365 (28.77%), Postives = 170/365 (46.58%), Query Frame = 1

Query: 359 IFKVKRLSEKDSLSLFKDYASTYEGNEKDIM---------KTLKKCNGIPLAIKCLGSML 418
           +  ++ LS+ D  SLF     T  GN++  +         + + KC G+PLA+K LG +L
Sbjct: 328 VHNLQPLSDGDCWSLFM---KTVFGNQEPCLNREIGDLAERIVHKCRGLPLAVKTLGGVL 387

Query: 419 SLGPPATKWMEDNERQKGD--NESSSTFSILKLCYNEMPSHLKRCFLYCSQLPNDSILSS 478
                  +W      +  D   + S+   +L++ Y  +P+HLKRCF YCS  P       
Sbjct: 388 RFEGKVIEWERVLSSRIWDLPADKSNLLPVLRVSYYYLPAHLKRCFAYCSIFPKGHAFEK 447

Query: 479 NDVIQLWMANGLLRSRQENYLSLEDIGEIYFKELCSRCFLQDVEEYGLGYWFKMHPLIRE 538
           + V+ LWMA G L+  + +  +LE++G  YF EL SR  LQ  +       + MH  I E
Sbjct: 448 DKVVLLWMAEGFLQQTRSS-KNLEELGNEYFSELESRSLLQKTKTR-----YIMHDFINE 507

Query: 539 LARL----------------VQKRTKDLISIK-------PVTNVTSIAFPVRDEVPSSSF 598
           LA+                 V +RT+ L  ++           +  + F +R  +P S  
Sbjct: 508 LAQFASGEFSSKFEDGCKLQVSERTRYLSYLRDNYAEPMEFEALREVKF-LRTFLPLSLT 567

Query: 599 LAEK--CISKFQHLRLLYLGHTDLQEI--------------PNTIETLNHLTYLDLQGNK 658
            + +  C+ +    +LL      L  +              P+  + ++H  +LDL    
Sbjct: 568 NSSRSCCLDQMVSEKLL----PTLTRLRVLSLSHYKIARLPPDFFKNISHARFLDL-SRT 627

Query: 659 NIKRLPNAICNLQHLQTLILASCSALEELPKDICKLSNLRYLWVTSNKLRLHKNGVGTMT 674
            +++LP ++C + +LQTL+L+ CS+L+ELP DI  L NLRYL +   KLR      G + 
Sbjct: 628 ELEKLPKSLCYMYNLQTLLLSYCSSLKELPTDISNLINLRYLDLIGTKLRQMPRRFGRLK 677

HSP 2 Score: 91.7 bits (226), Expect = 4.0e-17
Identity = 78/294 (26.53%), Postives = 141/294 (47.96%), Query Frame = 1

Query: 21  NYPRRIEYTMLSLKSILMDAEEKQEQSRGLQNWLEELQNVFSQIEGFIDEHKEEAYE-GI 80
           N   R+   +L++ ++L+DAEEKQ  +  ++ W+ EL++V    E  +D+   EA    I
Sbjct: 37  NLLERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDIATEALRLNI 96

Query: 81  GKQVLAPFSCSSNQIARTWKMEKLFDHLNEVAAKMYEFNLTERHTGAIKTETTNSFLTAT 140
           G +     S SSN++ +      L D L+  +  + E  L +      +  +  + L   
Sbjct: 97  GAE-----SSSSNRLRQLRGRMSLGDFLDGNSEHL-ETRLEKVTIRLERLASQRNILGLK 156

Query: 141 EVSTRLMKPSWKVLYPLTNAPKFYQDERYRKILN-----DFKNPTLGFFHIVGEAGIGKS 200
           E++  + K        +  +  F +D+   +I+      + K+  +    IVG  G+GK+
Sbjct: 157 ELTAMIPKQRLPTTSLVDESEVFGRDDDKDEIMRFLIPENGKDNGITVVAIVGIGGVGKT 216

Query: 201 TLAKFIYNDPEVEGMFPSRLWVCVKEEFDTQRLMKEILNFSYSPATCDNLTTKLCPTDQY 260
           TL++ +YND  V   F +++W  V EEFD  ++ K++   S +   C+   T L      
Sbjct: 217 TLSQLLYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYE-SVTSRPCE--FTDLDVLQVK 276

Query: 261 LRER------TFLLVFQDLSIKNLDNCSLFTSLLMMGKPGSKIIVTTQNEEIAN 303
           L+ER       FLLV  DL  +N  +  L     +    GS+I+VTT+++ +A+
Sbjct: 277 LKERLTGTGLPFLLVLDDLWNENFADWDLLRQPFIHAAQGSQILVTTRSQRVAS 321

BLAST of Cucsa.337190 vs. TrEMBL
Match: A0A0A0LEM8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G814400 PE=3 SV=1)

HSP 1 Score: 1577.8 bits (4084), Expect = 0.0e+00
Identity = 784/786 (99.75%), Postives = 786/786 (100.00%), Query Frame = 1

Query: 1   MAYCIYYRAENILSELKNLPNYPRRIEYTMLSLKSILMDAEEKQEQSRGLQNWLEELQNV 60
           MAYCIYYRAENILSELKNLPNYPRRIEYTMLSLKSILMDAEEKQEQSRGLQNWLEELQNV
Sbjct: 1   MAYCIYYRAENILSELKNLPNYPRRIEYTMLSLKSILMDAEEKQEQSRGLQNWLEELQNV 60

Query: 61  FSQIEGFIDEHKEEAYEGIGKQVLAPFSCSSNQIARTWKMEKLFDHLNEVAAKMYEFNLT 120
           FSQIEGFIDEHKEEAYEGIGKQVLAPFSCSSNQIARTWKMEKLFDHLNEVAAKMYEFNLT
Sbjct: 61  FSQIEGFIDEHKEEAYEGIGKQVLAPFSCSSNQIARTWKMEKLFDHLNEVAAKMYEFNLT 120

Query: 121 ERHTGAIKTETTNSFLTATEVSTRLMKPSWKVLYPLTNAPKFYQDERYRKILNDFKNPTL 180
           ERHTGAIKTETTNSFLTATEVSTRLMKPSWKVLYPLTNAPKFYQDERYRKILNDFKNPTL
Sbjct: 121 ERHTGAIKTETTNSFLTATEVSTRLMKPSWKVLYPLTNAPKFYQDERYRKILNDFKNPTL 180

Query: 181 GFFHIVGEAGIGKSTLAKFIYNDPEVEGMFPSRLWVCVKEEFDTQRLMKEILNFSYSPAT 240
           GFFHIVGEAGIGKSTLAKFIYNDPEVEGMFPSRLWVCVKEEFDTQRLMKEILNFSYSPAT
Sbjct: 181 GFFHIVGEAGIGKSTLAKFIYNDPEVEGMFPSRLWVCVKEEFDTQRLMKEILNFSYSPAT 240

Query: 241 CDNLTTKLCPTDQYLRERTFLLVFQDLSIKNLDNCSLFTSLLMMGKPGSKIIVTTQNEEI 300
           CDNLTTKLCPTDQYLRERTFLLVFQDLSIKNLDNCSLFTSLLMMGKPGSKIIVTTQNEEI
Sbjct: 241 CDNLTTKLCPTDQYLRERTFLLVFQDLSIKNLDNCSLFTSLLMMGKPGSKIIVTTQNEEI 300

Query: 301 ANAIELTMIYKVGQQSEQNRSQTALDTVTKETANVNNADQFVQANPLGKIDQSIPSQTIF 360
           ANAIELTMIYKVGQQSEQNRSQTALDTVTKETANVNNADQFVQANPLGKIDQSIPSQTIF
Sbjct: 301 ANAIELTMIYKVGQQSEQNRSQTALDTVTKETANVNNADQFVQANPLGKIDQSIPSQTIF 360

Query: 361 KVKRLSEKDSLSLFKDYASTYEGNEKDIMKTLKKCNGIPLAIKCLGSMLSLGPPATKWME 420
           KVKRLSEKDSLSLFKDYASTYEGNEKDIMKTLKKCNGIPLAIKCLGSMLSLGPPATKWME
Sbjct: 361 KVKRLSEKDSLSLFKDYASTYEGNEKDIMKTLKKCNGIPLAIKCLGSMLSLGPPATKWME 420

Query: 421 DNERQKGDNESSSTFSILKLCYNEMPSHLKRCFLYCSQLPNDSILSSNDVIQLWMANGLL 480
           DNERQKGDNESSSTFSILKLCYNEMPSHLKRCFLYCSQLPNDSILSSNDVIQLWMANGLL
Sbjct: 421 DNERQKGDNESSSTFSILKLCYNEMPSHLKRCFLYCSQLPNDSILSSNDVIQLWMANGLL 480

Query: 481 RSRQENYLSLEDIGEIYFKELCSRCFLQDVEEYGLGYWFKMHPLIRELARLVQKRTKDLI 540
           RSRQENYLSLEDIGEIYFKELCSRCFLQDVEEYGLGYWFKMHPLIRELARLVQKRTKDLI
Sbjct: 481 RSRQENYLSLEDIGEIYFKELCSRCFLQDVEEYGLGYWFKMHPLIRELARLVQKRTKDLI 540

Query: 541 SIKPVTNVTSIAFPVRDEVPSSSFLAEKCISKFQHLRLLYLGHTDLQEIPNTIETLNHLT 600
           SIKPVTNVTSIAFPVRDEVPSSSFLAEKCISKFQHLRLLYLGHTDLQEIPNTIETLNHLT
Sbjct: 541 SIKPVTNVTSIAFPVRDEVPSSSFLAEKCISKFQHLRLLYLGHTDLQEIPNTIETLNHLT 600

Query: 601 YLDLQGNKNIKRLPNAICNLQHLQTLILASCSALEELPKDICKLSNLRYLWVTSNKLRLH 660
           YLDLQGNKNIKRLPNAICNLQHLQTLILASCSALEELPKDICKLSNLRYLWVTSNKLRLH
Sbjct: 601 YLDLQGNKNIKRLPNAICNLQHLQTLILASCSALEELPKDICKLSNLRYLWVTSNKLRLH 660

Query: 661 KNGVGTMTSLRFLAIGGCDKLQDLFERPSCLVRLETLMIYDCNSLQLLPNEMGSLISLQN 720
           KNGVGTMTSLRFLAIGGCDKLQDLFERPSCLVRLETLMIYDCNSLQLLPNEMGSLISLQN
Sbjct: 661 KNGVGTMTSLRFLAIGGCDKLQDLFERPSCLVRLETLMIYDCNSLQLLPNEMGSLISLQN 720

Query: 721 LVIWSCKQLTLKGLEKVDFSLQRFTIRELPEVNKLPEWLQRSTETLRVLEIIDCPIKVEE 780
           LVIWSCKQLTLKGLEKVDFSLQRFTIRELPEVNKLPEWLQRSTETLRVLEIIDCPIKVEE
Sbjct: 721 LVIWSCKQLTLKGLEKVDFSLQRFTIRELPEVNKLPEWLQRSTETLRVLEIIDCPIKVEE 780

Query: 781 EGIKIH 787
           EGIK++
Sbjct: 781 EGIKMY 786

BLAST of Cucsa.337190 vs. TrEMBL
Match: A0A0A0LF99_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G814390 PE=3 SV=1)

HSP 1 Score: 864.8 bits (2233), Expect = 8.4e-248
Identity = 480/801 (59.93%), Postives = 581/801 (72.53%), Query Frame = 1

Query: 1   MAYCIYYRAENILSELKNLPNYPRRIEYTMLSLKSILMDAEEKQEQSRGLQNWLEELQNV 60
           MA CIY +AENIL ELK  P Y RR++YTMLSLK+IL DAE K+E    L +WL++LQ+V
Sbjct: 1   MACCIYEQAENILIELKKFPMYLRRMQYTMLSLKTILKDAE-KEEYRHCLNDWLQKLQSV 60

Query: 61  FSQIEGFIDEHKEEA--YEGIGKQVLAPFSCSSNQIARTWKMEKLFDHLNEVAAKMYEFN 120
           F QIE  + E   E    E  GK V  P S + +QI +T KM KL D L+E+A+ MY FN
Sbjct: 61  FLQIEELLYESNREVKKQEATGKWVFLP-SFNFSQIDQTKKMMKLCDDLDEIASHMYGFN 120

Query: 121 LTERHTGAIKTETTNSFLTATEVSTRLMKPSWKVLYPLTNAPKFYQDERYRKILNDFKNP 180
           LT         ETT+SFL+ATEVSTRLMKPSW++LY LTNAPK +QD+RY   L+ FK  
Sbjct: 121 LTNM-------ETTHSFLSATEVSTRLMKPSWQLLYSLTNAPKVFQDKRYHNFLDHFKKS 180

Query: 181 TLGFFHIVGEAGIGKSTLAKFIYNDPEVEGMFPSRLWVCVKEEFDTQRLMKEILNFSYSP 240
           T G FHIVGE GIGK+TLAKF YN+  +   FPSRLW+CVKEEFD QRL+KE+L+FS+  
Sbjct: 181 THGLFHIVGEPGIGKTTLAKFFYNN--LVNTFPSRLWICVKEEFDPQRLIKEMLSFSHCQ 240

Query: 241 ATCDNLTTK-LC-PTDQYLRERTFLLVFQDLSIKNLDNCSLFTSLLMMGKPGSKIIVTTQ 300
            TCDNLT K LC    Q+LR++ FL+VFQD+SIKNL NCS+F SLL MG  GSKIIVTTQ
Sbjct: 241 VTCDNLTEKQLCFAVQQFLRDKKFLIVFQDISIKNLGNCSIFKSLLGMGNRGSKIIVTTQ 300

Query: 301 NEEIANAIELTMIYKVGQQSEQNRSQTALDTVTKETANVNNADQFVQANPLGKIDQSIPS 360
           NE+IA+A+ L  +YK       N SQ        + ++VN                ++  
Sbjct: 301 NEKIADAVGLKKLYK-------NESQVVPSPEATKPSDVNK--------------DNMKH 360

Query: 361 QTIFKVKRLSEKDSLSLFKDYASTYEGNEKDIMKTLK-------KCNGIPLAIKCLGSML 420
           QTIFKV+RLS+++SLSLFK +A T E  E  I    K       KC+G+PLAIKCLG +L
Sbjct: 361 QTIFKVERLSKENSLSLFKVHAFT-ETQEAQIPNLTKIQEVIEQKCHGVPLAIKCLGGLL 420

Query: 421 SLGPPAT------KWMEDNERQKGDNESSSTFSILKLCYNEMPSHLKRCFLYCSQLPNDS 480
           S    A       K  E  E + G+    S    L+LCY++MPSHL+RCFLYCSQL  D 
Sbjct: 421 SKTSIAEWNGVIDKLWEHEEEEDGNK---SILPTLRLCYDQMPSHLQRCFLYCSQLKKDR 480

Query: 481 ILSSNDVIQLWMANGLLRSRQENYLSLEDIGEIYFKELCSRCFLQDVEEYGLGYWFKMHP 540
           ILSSNDVIQLW+A+ LL   +ENYLSLE IGE YFKELCSRCFLQ++EEYG GYWFK+HP
Sbjct: 481 ILSSNDVIQLWIASDLLP--KENYLSLEKIGENYFKELCSRCFLQELEEYGFGYWFKLHP 540

Query: 541 LIRELARLVQKRTKDLISIKPVTNVTSIAFPVRDEVPSSSFLAEKCISKFQHLRLLYLGH 600
           LI +LARL+ ++      +  VT   SIAF +RD+VP S+FLA  CI KF++LRLL+LG+
Sbjct: 541 LIEKLARLLTQK-----QVFEVTKTQSIAFTIRDKVPPSAFLANACIDKFKYLRLLHLGN 600

Query: 601 TDLQEIPNTIETLNHLTYLDLQGNKNIKRLPNAICNLQHLQTLILASCSALEELPKDICK 660
            +LQ IP+ +E L  L YLDLQGNK IKRLPN+I  L++LQTLILASCSAL+ELP DI +
Sbjct: 601 ANLQGIPSAVENLVQLRYLDLQGNKKIKRLPNSIFKLKNLQTLILASCSALKELPNDIRQ 660

Query: 661 LSNLRYLWVTSNKLRLHKNGVGTMTSLRFLAIGGCDKLQDLFERPSCLVRLETLMIYDCN 720
           L+NLRYLWVT+N LRLHKNGVGTMTSLRFLAIGGC  LQDLF++PSCLVRLETLMIYDC 
Sbjct: 661 LTNLRYLWVTANNLRLHKNGVGTMTSLRFLAIGGCQNLQDLFKKPSCLVRLETLMIYDCK 720

Query: 721 SLQLLPNEMGSLISLQNLVIWSCKQLTLKGLEKVDFSLQRFTIRELPEVNKLPEWLQRST 780
           +L+ LPNE+GSLISL+NLVIWSCK+LTL  L+ V+F LQRFTIRELP V KLPEW QR T
Sbjct: 721 TLKSLPNEIGSLISLKNLVIWSCKKLTLT-LKGVEFRLQRFTIRELPIVKKLPEWTQRFT 757

Query: 781 ETLRVLEIIDCPIKVEEEGIK 785
           ETLRVLEIIDCPI+  ++ +K
Sbjct: 781 ETLRVLEIIDCPIEWNDDVLK 757

BLAST of Cucsa.337190 vs. TrEMBL
Match: A0A0A0LBH9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G815400 PE=3 SV=1)

HSP 1 Score: 711.4 bits (1835), Expect = 1.2e-201
Identity = 419/867 (48.33%), Postives = 560/867 (64.59%), Query Frame = 1

Query: 1   MAYCIYYRAENILSELKNLPNYPRRIEYTMLSLKSILMDAEEKQEQSRGLQNWLEELQNV 60
           MA+CIY +AENIL++LK+ P Y +RIEY M SLK++L+DAEEKQEQ++ LQNWL+ELQNV
Sbjct: 1   MAHCIYTQAENILTKLKDSPPYQKRIEYAMSSLKAVLLDAEEKQEQNQRLQNWLKELQNV 60

Query: 61  FSQIEGFIDEHKEEAYE--GIGKQVLAPFSCSS----NQIARTWKMEKLFDHLNEVAAKM 120
           F Q+E  IDE K E ++   IGKQVLAPFSCS+    N++ +  K +++ D LN +A +M
Sbjct: 61  FYQVEDSIDEFKWEIFKQKDIGKQVLAPFSCSNQISANKLKQKCKRKQVCDELNNIATRM 120

Query: 121 YEFNLTERHTGAIKTETTNSFLTATEVSTRLMKPSWKVLYPLTNAPKFYQDERYRKILND 180
           YEF+L  +H  +I  ETT++F +A+E+S R +KPSW++LYPL +A + Y DE Y  ILN 
Sbjct: 121 YEFHLKVKHIDSISMETTHTFPSASEISIRHLKPSWQLLYPLIDASRTY-DEIYDGILNV 180

Query: 181 FKNPTLGFFHIVGEAGIGKSTLAKFIYNDPEVEGMFPSRLWVCVKEEFD----------- 240
           F N     FHIVGEAGIGKST+A+F+YN   V G + SR WVCV+E F+           
Sbjct: 181 F-NECTHVFHIVGEAGIGKSTVARFLYNHNNVVGKYTSRYWVCVEEGFNKHRLVKEVYSH 240

Query: 241 -----------TQRLMKEILNF-------------------SYSPATCDNLT------TK 300
                      T++L+ ++                        SP   + L       +K
Sbjct: 241 ADNKEICEDLTTEQLLSKVKRLLKVETFLLVFQDLSITNLKDRSPLVLNELLEMGQHGSK 300

Query: 301 LCPTDQ------YLRERTFLLVFQDLSIKNL--------DNCSLFTSLLMMGKPGSKIIV 360
           +  T Q      YL++R +    +  S +NL         N SL  +      PGS+I  
Sbjct: 301 IIVTTQTEEIANYLQDRGYKT--ERRSKENLANGDRVSAHNQSLTKNTQNQAVPGSQI-- 360

Query: 361 TTQNEEIANAIELTMI-YKVGQQSEQNRSQTALDTVTKETANVN---NADQFVQANPLGK 420
               EE A+AI   +  + +    +    +  +  VT++T N+    N D      P+ +
Sbjct: 361 ----EETADAIANKIYEHSLSLTEDAQNLEVPIPQVTEDT-NIGLGINPD----IPPIKQ 420

Query: 421 IDQSIPSQTIFKVKRLSEKDSLSLFKDYA----STYEGNE--KDIMKTLKKCNGIPLAIK 480
            D     QTIFK+++LS++ S  LFK+YA       E  E  K + + L+KC G+PLAIK
Sbjct: 421 DDTEY--QTIFKLEKLSKQSSSRLFKEYAFRNRQEVENPELTKIVDQLLEKCMGVPLAIK 480

Query: 481 CLGSMLSLGPPATKWMEDNERQK-GDNESSSTFSILKLCYNEMPSHLKRCFLYCSQLPND 540
           CLGS+LS      KW    E+    + + +    +L++CY++MPS LK CFL+CSQLPND
Sbjct: 481 CLGSLLSSETSIAKWKNIEEKLSLQEKKENGILHVLRVCYDQMPSQLKPCFLHCSQLPND 540

Query: 541 SILSSNDVIQLWMANGLLRSRQENYLSLEDIGEIYFKELCSRCFLQDVEEYGLGYWFKMH 600
            I+SSND+IQLWMANGLL S +E   ++E+IGE YF EL SR F+Q++EE+GLGYW K+H
Sbjct: 541 RIISSNDMIQLWMANGLLHSPEEKNSTMENIGEKYFMELWSRYFIQEIEEHGLGYWIKLH 600

Query: 601 PLIRELARLVQKRTKDLIS----IKPVTNVTSIAFPVRDEVPSSSFLAEKCISKFQHLRL 660
           PLI++LA  + +   +        K VT + SIAF  R+ V  ++ L EKCI K++ LRL
Sbjct: 601 PLIQKLAHKITQEQSEGSGGNNHPKEVTEIRSIAFQERNMVLPNASLTEKCIWKYKGLRL 660

Query: 661 LYLGHTDLQEIPNTIETLNHLTYLDLQGNKNIKRLPNAICNLQHLQTLILASCSALEELP 720
           LYL + DLQEIPN+I TL +L YLDL GN  IK LPN+ICNLQ LQTLIL SCSALE+LP
Sbjct: 661 LYLSNADLQEIPNSIGTLKYLRYLDLHGNTKIKHLPNSICNLQSLQTLILGSCSALEDLP 720

Query: 721 KDICKLSNLRYLWVTSNKLRLHKNGVGTMTSLRFLAIGGCDKLQDLFERPSCLVRLETLM 780
           KDI  L +LRYLWVT+NKLRL KNGVGTM SLRFLAIGGC+ L++LFERP CL  LETLM
Sbjct: 721 KDIRNLISLRYLWVTTNKLRLDKNGVGTMNSLRFLAIGGCNNLENLFERPDCLAGLETLM 780

Query: 781 IYDCNSLQLLPNEMGSLISLQNLVIWSCKQLTLKGLEKVDFSLQRFTIRELPEVNKLPEW 785
           IY+C +L+LLP+EM  L SLQNL+IWSCKQLTL  L++V+F LQRFTI+ELP V +LP+W
Sbjct: 781 IYNCTTLKLLPDEMRYLKSLQNLMIWSCKQLTL-NLKEVEFKLQRFTIKELPRVERLPQW 840

BLAST of Cucsa.337190 vs. TrEMBL
Match: M5WZI0_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa019872mg PE=3 SV=1)

HSP 1 Score: 320.1 bits (819), Expect = 7.7e-84
Identity = 251/825 (30.42%), Postives = 408/825 (49.45%), Query Frame = 1

Query: 1   MAYCIYYRAENILSELKN-----------LPNYPRRIEYTMLSLKSILMDAEEKQEQSRG 60
           M   +Y  AEN+++ L +           + +   +++ T  S+K +++DAEEKQ +   
Sbjct: 1   MGQFVYTIAENVMNGLASYAYQEMCLAWGVTDELNKLKDTFSSIKLVILDAEEKQRKELH 60

Query: 61  LQNWLEELQNVFSQIEGFIDEHKEEAYE---------GIGKQVLAPFSCSSNQIARTWKM 120
           L  WL +L++V   ++  +++   +  +         G  K+ +  F    N +   +KM
Sbjct: 61  LSQWLGKLKDVCYDVDDMLNDFAFKELQMQMLILRGRGSIKEQVRIFFSGWNPVVFNFKM 120

Query: 121 ----EKLFDHLNEVAAKMYEFNLTER------HTGAIKTETTNSFLTATEVSTRLMKPSW 180
               + + + L+ +A +  +F+L ER       +  IK ET +SF+ A +V  R      
Sbjct: 121 GPRIKDIKERLDVIAIEKSQFHLVERAGDWQSQSVHIKRET-HSFVQAYDVIGREDDKKQ 180

Query: 181 KVLYPLTNAPKFYQDERYRKILNDFKNPTLGFFHIVGEAGIGKSTLAKFIYNDPEVEGMF 240
            +++      K    E+ R +  +     +    I G  GIGK+TLAK +YND  V   F
Sbjct: 181 IIIHLFNLTLK----EKSRDVQEN-----VSVISITGLGGIGKTTLAKLVYNDHRVVKHF 240

Query: 241 PSRLWVCVKEEFDTQRLMKEILNFSYSPATC------DNLTTKLCPTDQYLRERTFLLVF 300
             R+WVCV + FD +RL++EI+  S +   C      D +  KL      L+++ FLLV 
Sbjct: 241 DMRMWVCVSDHFDNKRLVREIVA-SATNQNCGDQESFDRVQKKL---QSALKDKQFLLVL 300

Query: 301 QDLSIKNLDNCSLFTSLLMMGKPGSKIIVTTQNEEIANAIELTMIYKVGQQSEQNRSQTA 360
            D+  K                   +I VTT     A  I+L  +  VG     N S+  
Sbjct: 301 DDVWDKG------------------QIGVTT-----AKWIDLKALLNVGA----NGSKVI 360

Query: 361 LDTVTKETANVNNADQFVQANPLGKIDQSIPSQTIFKVKRLSEKDSLSLFKDYASTYEGN 420
           + T ++  A++                  + S  + +++ L  +  +SLF   A   +G 
Sbjct: 361 VTTRSESVASI------------------MDSVCVHQLRGLPHESCMSLFIKRAFGRKGE 420

Query: 421 EK---DIMKTL----KKCNGIPLAIKCLGSMLSLGPPATKWME--DNERQKGDNESSSTF 480
           E+    +M+      +KC G+PLA+  +GS+L L      W E  DN+  +    +    
Sbjct: 421 EQRHPHLMEIAHGIAEKCEGVPLAVTTMGSLLCLKREKHFWSEVRDNDIWRLPQGNDDIL 480

Query: 481 SILKLCYNEMPSHLKRCFLYCSQLPNDSILSSNDVIQLWMANGLLRSRQENYLSLEDIGE 540
             LKL Y+ +PS+LK CF  CS  P D +  S D++ LWMA G ++S + N   LE+IG 
Sbjct: 481 PALKLSYDALPSYLKPCFAICSLFPKDYVFRSTDLVSLWMAQGFIQSSKGNQ-ELEEIGL 540

Query: 541 IYFKELCSRCFLQDVEEYGLGYWFKMHPLIRELARLVQKRTKDLISIKPVTN-----VTS 600
            Y ++LCSR   Q  E+      FKMH L+ +LA  V +     ++ +P ++     V +
Sbjct: 541 DYIRQLCSRSLFQIDEDSIDFIVFKMHALVHDLAMSVSEEECSSVNFRPTSDTCKRKVWT 600

Query: 601 IAFPVRDEVPSSS-FLAEKCISKFQHLRLLYLGHTDLQEIPNTIETLNHLTYLDLQGNKN 660
           I FPV   V +SS  + + CI +F++LR+L L     + +P++I  L+ L YLDL  N  
Sbjct: 601 ILFPVPGHVGTSSKSVLKACILRFKYLRVLDLSGLTFEVLPSSIGKLSRLRYLDLSKNPF 660

Query: 661 IKRLPNAICNLQHLQTLILASCSALEELPKDICKLSNLRYLWVTSNKLRLHKNGVGTMTS 720
           IK+LP +ICNL +LQTL+L++C  L ELP+DI  L NLR L +T+N+ ++   G+  +TS
Sbjct: 661 IKKLPGSICNLLNLQTLLLSNCEKLRELPRDIGNLINLRTLVLTTNQ-KVLAGGIERLTS 720

Query: 721 LRFLAIGGCDKLQDLFERPSCLVRLETLMIYDCNSLQLLPNEMGSLISLQNLVIWSCKQL 775
           LRFL +  C  L+ L +   CL  L  L+I +C +L+ LP +M  L +L+ L I  C++L
Sbjct: 721 LRFLQVHNCSYLESLGQGIQCLTNLRMLVISNCENLKSLPPDMKCLTALKTLGISDCEKL 764

BLAST of Cucsa.337190 vs. TrEMBL
Match: M5WZI0_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa019872mg PE=3 SV=1)

HSP 1 Score: 33.5 bits (75), Expect = 1.4e+03
Identity = 20/66 (30.30%), Postives = 37/66 (56.06%), Query Frame = 1

Query: 586 LQEIPNTIE-TLNHLTYLDLQGNKNIKRLPNAICNLQHLQTLILASCSALEELPKDICKL 645
           L+ +P+ ++ + N L  L ++  +++K LP  + N + LQ L++  C  L  LP+ +  L
Sbjct: 742 LEALPHWLQDSANTLQSLRVKNCEDLKELPEWLQNFKLLQQLVIEYCPQLLALPQGMYHL 801

Query: 646 SNLRYL 651
             LR L
Sbjct: 802 GALRLL 807

HSP 2 Score: 315.1 bits (806), Expect = 2.5e-82
Identity = 252/819 (30.77%), Postives = 390/819 (47.62%), Query Frame = 1

Query: 24  RRIEYTMLSLKSILMDAEEKQEQSRGLQNWLEELQNVFSQIEGFIDEHKEEAYEG----- 83
           ++++++ML +K +L+DAEEK+  S  L+ WL++L +VF   E  +DE + E         
Sbjct: 35  KKLKHSMLVIKDVLIDAEEKRSNSPELRLWLKQLNHVFYDAEDVLDELEVENLRRQVIDR 94

Query: 84  ---IGKQVLAPFSCSSNQIARTW---KMEKLFDHLNEVAAKMYEFNLTER---HTGAIKT 143
                ++VL  FS S+  I R+    K++++ + L+ +AA   +  LTER        + 
Sbjct: 95  GNFYTRKVLRCFSSSNPLIFRSTIGRKLKRINEGLDAIAAGNVKCRLTERAEERRPLNRE 154

Query: 144 ETTNSFLTATEVSTRLMKPSWKVLYPLTNAPKFYQDERYRKILNDFKNPT----LGFFHI 203
             T+SF+ +  +  R                    DE   KI+    +P+    +    I
Sbjct: 155 RGTHSFVHSAGIIGR--------------------DEDKEKIIQLLLHPSDEENISVLPI 214

Query: 204 VGEAGIGKSTLAKFIYNDPEVEGMFPSRLWVCVKEEFDTQRLMKEILNFSYSPATC--DN 263
           VG  G+GK+TLAK  YND  V   F  ++WV V  + D +RLM++++  +        DN
Sbjct: 215 VGIGGMGKTTLAKMAYNDERVVKHFQFKMWVYVSRDSDKKRLMEKLIISATGGVGIGEDN 274

Query: 264 LTTKLCPTDQYLRE----RTFLLVFQDLSIKNLDNCSLFTSLLMMGKPGSKIIVTTQNEE 323
            + ++      LRE    + + LV  DL                             N+ 
Sbjct: 275 GSMEVEELQTLLRESIRDKKYFLVLDDL----------------------------WNDN 334

Query: 324 IANAIELTMIYKVGQQSEQNRSQTALDTVTKETAN-VNNADQFVQANPLGKIDQSIPSQT 383
           +A   EL  + +VG +     S   + T + + A+ +  A ++V                
Sbjct: 335 LARWEELKDLLRVGARG----SMIMVTTRSNQVASMIGTAPKYVH--------------- 394

Query: 384 IFKVKRLSEKDSLSLFKDYASTYEGNEKDIMKTL-------KKCNGIPLAIKCLGSMLSL 443
              ++ +   + LSLF  YA   EG +K     L       KKC  +PLA++ L   L L
Sbjct: 395 --NLQGVRYDECLSLFVKYAFK-EGQDKQYPNLLRIGEEIVKKCGEVPLAVRTLAGQLFL 454

Query: 444 GPPATKW--MEDNERQKGDNESSSTFSILKLCYNEMPSHLKRCFLYCSQLPNDSILSSND 503
                 W  + D+   K + +       L++ Y ++PS LKRCF YCS  P +   +  +
Sbjct: 455 NTDERDWNLIRDSRLWKVEQKEDDILPALRVSYEQLPSCLKRCFAYCSLFPKNYEYNDYE 514

Query: 504 VIQLWMANGLLRSRQENYLSLEDIGEIYFKELCSRCFLQDVEE-YG---LGYWFKMHPLI 563
           +IQ WMA+GLL+S  +    LEDIG IY KEL   CFLQD  + YG    G    MH L 
Sbjct: 515 LIQFWMAHGLLQS-SDGESELEDIGSIYLKELEYGCFLQDFRDLYGSLQFGMLDVMHDLA 574

Query: 564 RELAR----LVQKRTKDL------ISI--------------KPVTNVTSIAFPVRDEVPS 623
             +A+    +V   +K +      ISI              K +  V ++      +V +
Sbjct: 575 LSVAQDECFVVTANSKRIEKSVQHISIPDPDSVRQDFPMLSKELDQVRTVFIHSDKDVLA 634

Query: 624 SSFLAEKCISKFQHLRLLYLGHTDLQEIPNTIETLNHLTYLDLQGNKNIKRLPNAICNLQ 683
           S+ + E C+S+F++LR L L  +  +E+P  I  L HL YLDL  N  IKRLPN+IC LQ
Sbjct: 635 SNSILETCLSRFKYLRALNLSRSQFKELPKKIGKLKHLRYLDLSWNHRIKRLPNSICKLQ 694

Query: 684 HLQTLILASCSALEELPKDICKLSNLRYLWVTSNKLRLHKNGVGTMTSLRFLAIGGCDKL 743
           +LQTL L  C  +EELP+ +  + +LR+LW+ + +  L ++ +G + SLRFL I  C+ L
Sbjct: 695 NLQTLFLGGCDEIEELPRGMRYMESLRFLWLATRQTSLPRDEIGCLKSLRFLWIATCENL 754

Query: 744 QDLFERPSCLVRLETLMIYDCNSLQLLPNEMGSLISLQNLVIWSCKQLTLKGLEKVDFSL 780
           + LFE    L  L +L I  C SL  LP  +  L SLQ+L I  C  L     E  +F L
Sbjct: 755 ERLFEDMENLSALRSLYIVTCPSLNSLPPSIKYLTSLQDLHISGCVALNFPNQEACEFKL 782

BLAST of Cucsa.337190 vs. TAIR10
Match: AT3G14470.1 (AT3G14470.1 NB-ARC domain-containing disease resistance protein)

HSP 1 Score: 129.4 bits (324), Expect = 9.7e-30
Identity = 105/365 (28.77%), Postives = 170/365 (46.58%), Query Frame = 1

Query: 359 IFKVKRLSEKDSLSLFKDYASTYEGNEKDIM---------KTLKKCNGIPLAIKCLGSML 418
           +  ++ LS+ D  SLF     T  GN++  +         + + KC G+PLA+K LG +L
Sbjct: 328 VHNLQPLSDGDCWSLFM---KTVFGNQEPCLNREIGDLAERIVHKCRGLPLAVKTLGGVL 387

Query: 419 SLGPPATKWMEDNERQKGD--NESSSTFSILKLCYNEMPSHLKRCFLYCSQLPNDSILSS 478
                  +W      +  D   + S+   +L++ Y  +P+HLKRCF YCS  P       
Sbjct: 388 RFEGKVIEWERVLSSRIWDLPADKSNLLPVLRVSYYYLPAHLKRCFAYCSIFPKGHAFEK 447

Query: 479 NDVIQLWMANGLLRSRQENYLSLEDIGEIYFKELCSRCFLQDVEEYGLGYWFKMHPLIRE 538
           + V+ LWMA G L+  + +  +LE++G  YF EL SR  LQ  +       + MH  I E
Sbjct: 448 DKVVLLWMAEGFLQQTRSS-KNLEELGNEYFSELESRSLLQKTKTR-----YIMHDFINE 507

Query: 539 LARL----------------VQKRTKDLISIK-------PVTNVTSIAFPVRDEVPSSSF 598
           LA+                 V +RT+ L  ++           +  + F +R  +P S  
Sbjct: 508 LAQFASGEFSSKFEDGCKLQVSERTRYLSYLRDNYAEPMEFEALREVKF-LRTFLPLSLT 567

Query: 599 LAEK--CISKFQHLRLLYLGHTDLQEI--------------PNTIETLNHLTYLDLQGNK 658
            + +  C+ +    +LL      L  +              P+  + ++H  +LDL    
Sbjct: 568 NSSRSCCLDQMVSEKLL----PTLTRLRVLSLSHYKIARLPPDFFKNISHARFLDL-SRT 627

Query: 659 NIKRLPNAICNLQHLQTLILASCSALEELPKDICKLSNLRYLWVTSNKLRLHKNGVGTMT 674
            +++LP ++C + +LQTL+L+ CS+L+ELP DI  L NLRYL +   KLR      G + 
Sbjct: 628 ELEKLPKSLCYMYNLQTLLLSYCSSLKELPTDISNLINLRYLDLIGTKLRQMPRRFGRLK 677

HSP 2 Score: 91.7 bits (226), Expect = 2.2e-18
Identity = 78/294 (26.53%), Postives = 141/294 (47.96%), Query Frame = 1

Query: 21  NYPRRIEYTMLSLKSILMDAEEKQEQSRGLQNWLEELQNVFSQIEGFIDEHKEEAYE-GI 80
           N   R+   +L++ ++L+DAEEKQ  +  ++ W+ EL++V    E  +D+   EA    I
Sbjct: 37  NLLERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDIATEALRLNI 96

Query: 81  GKQVLAPFSCSSNQIARTWKMEKLFDHLNEVAAKMYEFNLTERHTGAIKTETTNSFLTAT 140
           G +     S SSN++ +      L D L+  +  + E  L +      +  +  + L   
Sbjct: 97  GAE-----SSSSNRLRQLRGRMSLGDFLDGNSEHL-ETRLEKVTIRLERLASQRNILGLK 156

Query: 141 EVSTRLMKPSWKVLYPLTNAPKFYQDERYRKILN-----DFKNPTLGFFHIVGEAGIGKS 200
           E++  + K        +  +  F +D+   +I+      + K+  +    IVG  G+GK+
Sbjct: 157 ELTAMIPKQRLPTTSLVDESEVFGRDDDKDEIMRFLIPENGKDNGITVVAIVGIGGVGKT 216

Query: 201 TLAKFIYNDPEVEGMFPSRLWVCVKEEFDTQRLMKEILNFSYSPATCDNLTTKLCPTDQY 260
           TL++ +YND  V   F +++W  V EEFD  ++ K++   S +   C+   T L      
Sbjct: 217 TLSQLLYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYE-SVTSRPCE--FTDLDVLQVK 276

Query: 261 LRER------TFLLVFQDLSIKNLDNCSLFTSLLMMGKPGSKIIVTTQNEEIAN 303
           L+ER       FLLV  DL  +N  +  L     +    GS+I+VTT+++ +A+
Sbjct: 277 LKERLTGTGLPFLLVLDDLWNENFADWDLLRQPFIHAAQGSQILVTTRSQRVAS 321

BLAST of Cucsa.337190 vs. TAIR10
Match: AT5G48620.1 (AT5G48620.1 Disease resistance protein (CC-NBS-LRR class) family)

HSP 1 Score: 107.8 bits (268), Expect = 3.0e-23
Identity = 157/696 (22.56%), Postives = 278/696 (39.94%), Query Frame = 1

Query: 32  SLKSILMDAEEKQEQSRGLQNWLEELQNVFSQIEGFIDEHKEEAYEGIGKQV------LA 91
           SL+S+L DA+ K+  S  ++N+LE+++++    E  I+ +      G GK V      LA
Sbjct: 40  SLQSLLKDADAKKHGSDRVRNFLEDVKDLVFDAEDIIESYVLNKLRGEGKGVKKHVRRLA 99

Query: 92  PFSCSSNQIARTWKMEKLFDHLNEVAAKMYEFNLTERHTGAIKTETTNSFLTATEVSTRL 151
            F    +++A    +E +   +++V  +M  F + +   G              E+    
Sbjct: 100 RFLTDRHKVASD--IEGITKRISDVIGEMQSFGIQQIIDGVRSLSLQERQRVQREI---- 159

Query: 152 MKPSWKVLYPLTNAPKFYQDERYRKIL--NDFKNPTLGFFHIVGEAGIGKSTLAKFIYND 211
                +  YP ++       E+  + L  +  +N       I G  GIGK+TLA+ +++ 
Sbjct: 160 -----RQTYPDSSESDLVGVEQSVEELVGHLVENDIYQVVSIAGMGGIGKTTLARQVFHH 219

Query: 212 PEVEGMFPSRLWVCVKEEFDTQRLMKEILNFSYSPATCDNLTTKLCPTDQYLRERTFLLV 271
             V   F    WVCV                                + Q+  +  +  +
Sbjct: 220 DLVRRHFDGFAWVCV--------------------------------SQQFTLKHVWQRI 279

Query: 272 FQDL-----SIKNLDNCSLFTSLLMMGKPGSKIIVTTQNEEIANAIELTMIYKVGQQSEQ 331
            Q+L     +I  +D  +L   L  + + G  ++V     +  +   +  ++       +
Sbjct: 280 LQELQPHDGNILQMDESALQPKLFQLLETGRYLLVLDDVWKKEDWDRIKAVFP------R 339

Query: 332 NRSQTALDTVTKETANVNNADQFVQANPLGKIDQSIPSQTIFKVKRLSEKDSLSLF---- 391
            R    L T   E   ++ AD               P+   F+   L+ ++S  L     
Sbjct: 340 KRGWKMLLTSRNEGVGIH-AD---------------PTCLTFRASILNPEESWKLCERIV 399

Query: 392 ---KDYASTYEGNEKDIM--KTLKKCNGIPLAIKCLGSMLSLGPPATKW--MEDNERQK- 451
              +D        E + M  + +  C G+PLA+K LG +L+      +W  + DN   + 
Sbjct: 400 FPRRDETEVRLDEEMEAMGKEMVTHCGGLPLAVKALGGLLANKHTVPEWKRVSDNIGSQI 459

Query: 452 ------GDNESSSTFSILKLCYNEMPSHLKRCFLYCSQLPNDSILSSNDVIQLWMANGLL 511
                  DN  +S   IL L Y ++P+HLK  FLY +  P DS + + D+   W A G+ 
Sbjct: 460 VGGSCLDDNSLNSVNRILSLSYEDLPTHLKHRFLYLAHFPEDSKIYTQDLFNYWAAEGIY 519

Query: 512 RSRQENYLSLEDIGEIYFKELCSR-CFLQDVEEYGLGYWF-KMHPLIRELARLVQKRTKD 571
                   +++D GE Y +EL  R   + D     L + F +MH ++RE+     K    
Sbjct: 520 DGS-----TIQDSGEYYLEELVRRNLVIADNRYLSLEFNFCQMHDMMREVCLSKAKEENF 579

Query: 572 LISIKPVTNVTSIAFPVRDEVPSSSFLAEKCISKFQHLRLLYLGHTDLQEIPNTI----- 631
           L  IK  T+ ++I      + PS S        K  H+    LGH +  ++ + I     
Sbjct: 580 LQIIKDPTSTSTI----NAQSPSRSRRFSIHSGKAFHI----LGHRNNPKVRSLIVSRFE 639

Query: 632 --------ETLNHLTYLDLQGNKNIK----RLPNAICNLQHLQTLILASCSALEELPKDI 676
                      ++LT L +     +K    +LP++I  L HL+ L L   + +  LP  +
Sbjct: 640 EDFWIRSASVFHNLTLLRVLDLSRVKFEGGKLPSSIGGLIHLRYLSLYG-AVVSHLPSTM 656

BLAST of Cucsa.337190 vs. TAIR10
Match: AT5G43470.1 (AT5G43470.1 Disease resistance protein (CC-NBS-LRR class) family)

HSP 1 Score: 104.4 bits (259), Expect = 3.4e-22
Identity = 161/697 (23.10%), Postives = 273/697 (39.17%), Query Frame = 1

Query: 32  SLKSILMDAEEKQEQSRGLQNWLEELQNVFSQIEGFIDEHKEEAYEGIGKQV------LA 91
           SL+S+L DA+ K+  S  ++N+LE+++++    E  I+ +      G GK V      LA
Sbjct: 40  SLQSLLKDADAKKHGSDRVRNFLEDVKDLVFDAEDIIESYVLNKLSGKGKGVKKHVRRLA 99

Query: 92  PFSCSSNQIARTWKMEKLFDHLNEVAAKMYEFNLTERHTGAIKTETTNSFLTATEVSTRL 151
            F    +++A    +E +   ++EV  +M  F + +   G              E+    
Sbjct: 100 CFLTDRHKVASD--IEGITKRISEVIGEMQSFGIQQIIDGGRSLSLQERQRVQREI---- 159

Query: 152 MKPSWKVLYPLTNAPKFYQDERYRKIL--NDFKNPTLGFFHIVGEAGIGKSTLAKFIYND 211
                +  YP ++       E+  K L  +  +N       I G  GIGK+TLA+ +++ 
Sbjct: 160 -----RQTYPDSSESDLVGVEQSVKELVGHLVENDVHQVVSIAGMGGIGKTTLARQVFHH 219

Query: 212 PEVEGMFPSRLWVCVKEEFDTQRLMKEILNFSYSPATCDNLTTKLCPTDQYLRERTFLLV 271
             V   F    WVCV                                + Q+ ++  +  +
Sbjct: 220 DLVRRHFDGFAWVCV--------------------------------SQQFTQKHVWQRI 279

Query: 272 FQDLS-----IKNLDNCSLFTSLLMMGKPGSKIIVTTQNEEIANAIELTMIYKVGQQSEQ 331
            Q+L      I  +D  +L   L  + + G  ++V    +++    +  +I  V     +
Sbjct: 280 LQELQPHDGDILQMDEYALQRKLFQLLEAGRYLVVL---DDVWKKEDWDVIKAV---FPR 339

Query: 332 NRSQTALDTVTKETANVNNADQFVQANPLGKIDQSIPSQTIFKVKRLSEKDSLSLF---- 391
            R    L T   E   ++ AD               P+   F+   L+ ++S  L     
Sbjct: 340 KRGWKMLLTSRNEGVGIH-AD---------------PTCLTFRASILNPEESWKLCERIV 399

Query: 392 ---KDYASTYEGNEKDIM--KTLKKCNGIPLAIKCLGSMLSLGPPATKWME--DNERQK- 451
              +D        E + M  + +  C G+PLA+K LG +L+      +W    DN   + 
Sbjct: 400 FPRRDETEVRLDEEMEAMGKEMVTHCGGLPLAVKALGGLLANKHTVPEWKRVFDNIGSQI 459

Query: 452 ------GDNESSSTFSILKLCYNEMPSHLKRCFLYCSQLPNDSILSSNDVIQLWMANGLL 511
                  DN  +S + IL L Y ++P+HLK CFL  +  P DS +S+  +   W A G+ 
Sbjct: 460 VGGSWLDDNSLNSVYRILSLSYEDLPTHLKHCFLNLAHFPEDSEISTYSLFYYWAAEGIY 519

Query: 512 RSRQENYLSLEDIGEIYFKELCSRCFLQDVEEY--GLGYWFKMHPLIRELARLVQKRTKD 571
                   ++ED GE Y +EL  R  +   + Y      + +MH ++RE+     K    
Sbjct: 520 DGS-----TIEDSGEYYLEELVRRNLVIADDNYLSWQSKYCQMHDMMREVCLSKAKEENF 579

Query: 572 L-ISIKPVTNVTSIAFPVRDEVPSSSFLAEKCISKFQHLRLLYLGHTDLQEIPNTI---- 631
           L I I P    T     +  + PS S        K  H+    LGH +  ++ + I    
Sbjct: 580 LQIIIDPTCTST-----INAQSPSRSRRLSIHSGKAFHI----LGHKNKTKVRSLIVPRF 639

Query: 632 ------------ETLNHLTYLDLQGNK-NIKRLPNAICNLQHLQTLILASCSALEELPKD 676
                         L  L  LDL   K    +LP +I  L HL+ L L   + +  LP  
Sbjct: 640 EEDYWIRSASVFHNLTLLRVLDLSWVKFEGGKLPCSIGGLIHLRYLSLYE-AKVSHLPST 656

BLAST of Cucsa.337190 vs. TAIR10
Match: AT3G07040.1 (AT3G07040.1 NB-ARC domain-containing disease resistance protein)

HSP 1 Score: 104.0 bits (258), Expect = 4.4e-22
Identity = 96/351 (27.35%), Postives = 160/351 (45.58%), Query Frame = 1

Query: 390 KTLKKCNGIPLAIKCLGSMLSLGPPATKWMED----NERQKGDNESSSTFSILKLCYNEM 449
           K +++C G+PLAI  LGSM+S     ++W +     N     ++E     SI+ L +N++
Sbjct: 368 KLVERCQGLPLAIASLGSMMSTKKFESEWKKVYSTLNWELNNNHELKIVRSIMFLSFNDL 427

Query: 450 PSHLKRCFLYCSQLPNDSILSSNDVIQLWMANGLLRSRQENYLSLEDIGEIYFKELCSRC 509
           P  LKRCFLYCS  P +  +    +I++WMA   +   +   +  E++ + Y  EL  R 
Sbjct: 428 PYPLKRCFLYCSLFPVNYRMKRKRLIRMWMAQRFVEPIRG--VKAEEVADSYLNELVYRN 487

Query: 510 FLQDV--EEYGLGYWFKMHPLIRELARLVQK--RTKDLI--------SIKPVTNVTSIAF 569
            LQ +    +G    FKMH +I E+A  V K  R  D+         + + + N  S   
Sbjct: 488 MLQVILWNPFGRPKAFKMHDVIWEIALSVSKLERFCDVYNDDSDGDDAAETMENYGSRHL 547

Query: 570 PVRDEVPSSSFLAE---------------KCISKFQHLRLLYLGHTDLQEIPNTIETLNH 629
            ++ E+   S  A                + +     LR L L  + + ++P+ + T+ +
Sbjct: 548 CIQKEMTPDSIRATNLHSLLVCSSAKHKMELLPSLNLLRALDLEDSSISKLPDCLVTMFN 607

Query: 630 LTYLDLQGNKNIKRLPNAICNLQHLQTLILASCSALEELPKDICKLSNLRYLWVTSNKLR 689
           L YL+L     +K LP     L +L+TL     S +EELP  + KL  LRYL +T  +  
Sbjct: 608 LKYLNL-SKTQVKELPKNFHKLVNLETL-NTKHSKIEELPLGMWKLKKLRYL-ITFRRNE 667

Query: 690 LHKNG---------VGTMTSLRFLAIGGCDKLQD-LFERPSCLVRLETLMI 700
            H +          V  +  L+ L +  C   +D L +   C+ +L  + +
Sbjct: 668 GHDSNWNYVLGTRVVPKIWQLKDLQVMDCFNAEDELIKNLGCMTQLTRISL 713

HSP 2 Score: 38.1 bits (87), Expect = 3.0e-02
Identity = 37/167 (22.16%), Postives = 72/167 (43.11%), Query Frame = 1

Query: 586 LQEIPNTIETLNHLTYLDLQGNKNIKRLPNAICNLQHLQTLILASCSALEELPKDICKLS 645
           L+ +P+   TL +LTYL L+G+   +   NAI ++Q L  L+  S               
Sbjct: 768 LERVPSWFNTLQNLTYLGLRGS---QLQENAILSIQTLPRLVWLSF-------------- 827

Query: 646 NLRYLWVTSNKLRLHKNGVGTMTSLRFLAIGGCDKLQDLFERPSCLVRLETLMIYDCNSL 705
              Y      +LR  +       +L+ L I     L ++      +  L+ L +  C  L
Sbjct: 828 ---YNAYMGPRLRFAQG----FQNLKILEIVQMKHLTEVVIEDGAMFELQKLYVRACRGL 887

Query: 706 QLLPNEMGSLISLQNLVIWSCKQLTLKGLEKVDFSLQRFTIRELPEV 753
           + +P  + +LI+LQ L +       ++ + + + S+ R  ++ +P +
Sbjct: 888 EYVPRGIENLINLQELHLIHVSNQLVERI-RGEGSVDRSRVKHIPAI 909

BLAST of Cucsa.337190 vs. TAIR10
Match: AT1G58807.1 (AT1G58807.1 Disease resistance protein (CC-NBS-LRR class) family)

HSP 1 Score: 103.6 bits (257), Expect = 5.7e-22
Identity = 111/419 (26.49%), Postives = 186/419 (44.39%), Query Frame = 1

Query: 360 FKVKRLSEKDSLSLF-------KDYASTYEGNEKDIMKTL--KKCNGIPLAIKCLGSMLS 419
           FK + L+ +DS +LF       KD A      EK+ +  L  K C G+PLAI+ LG ML+
Sbjct: 316 FKPECLTTEDSWTLFQRIALPMKDAAEFKIDEEKEELGKLMIKHCGGLPLAIRVLGGMLA 375

Query: 420 LGPPATKWMEDNER----------QKGDNESSSTFSILKLCYNEMPSHLKRCFLYCSQLP 479
               +  W   +E              D+ +++  ++L L + E+PS+LK CFLY +  P
Sbjct: 376 EKYTSHDWRRLSENIGSHLVGGRTNFNDDNNNTCNNVLSLSFEELPSYLKHCFLYLAHFP 435

Query: 480 NDSILSSNDVIQLWMANGLLRSRQENYLSLEDIGEIYFKELCSRCFLQDVEEYGLGYW-- 539
            D  +   ++   W A G+ + R  +  ++ D+G++Y +EL  R  +    +     +  
Sbjct: 436 EDYEIKVENLSYYWAAEGIFQPRHYDGETIRDVGDVYIEELVRRNMVISERDVKTSRFET 495

Query: 540 FKMHPLIRELARLVQKRTK--DLISIKPVT-----NVTSIAF--------PVRDEVPSSS 599
             +H ++RE+  L  K      + S +P T      VTS  F         V  ++ +  
Sbjct: 496 CHLHDMMREVCLLKAKEENFLQITSSRPSTANLQSTVTSRRFVYQYPTTLHVEKDINNPK 555

Query: 600 F------------LAEKCISKFQHLRLLYLGHTDLQ--EIPNTIETLNHLTYLDLQGNKN 659
                        LA    ++ + LR+L L    ++  ++ + I  L HL YL L+    
Sbjct: 556 LRALVVVTLGSWNLAGSSFTRLELLRVLDLIEVKIKGGKLASCIGKLIHLRYLSLE-YAE 615

Query: 660 IKRLPNAICNLQHLQTLILASCSALEELPKDICKLSNLRYLWVTSNKLRLHKNGVGTMTS 719
           +  +P ++ NL+ L  L LAS      +P  +  +  LRYL + S+  R  K  +  +  
Sbjct: 616 VTHIPYSLGNLKLLIYLNLASFGRSTFVPNVLMGMQELRYLALPSDMGRKTKLELSNLVK 675

Query: 720 LRFLAIGGCD--KLQDLFERPSCLVRLETLMI--YDCNSLQLLPNEMGSLISLQNLVIW 725
           L  L     +   L+DL      +VRL TL I   +  SL+ L   +G L  L+ L I+
Sbjct: 676 LETLENFSTENSSLEDL----CGMVRLSTLNIKLIEETSLETLAASIGGLKYLEKLEIY 729

BLAST of Cucsa.337190 vs. NCBI nr
Match: gi|449437958|ref|XP_004136757.1| (PREDICTED: putative disease resistance protein RGA3 [Cucumis sativus])

HSP 1 Score: 1577.8 bits (4084), Expect = 0.0e+00
Identity = 784/786 (99.75%), Postives = 786/786 (100.00%), Query Frame = 1

Query: 1   MAYCIYYRAENILSELKNLPNYPRRIEYTMLSLKSILMDAEEKQEQSRGLQNWLEELQNV 60
           MAYCIYYRAENILSELKNLPNYPRRIEYTMLSLKSILMDAEEKQEQSRGLQNWLEELQNV
Sbjct: 1   MAYCIYYRAENILSELKNLPNYPRRIEYTMLSLKSILMDAEEKQEQSRGLQNWLEELQNV 60

Query: 61  FSQIEGFIDEHKEEAYEGIGKQVLAPFSCSSNQIARTWKMEKLFDHLNEVAAKMYEFNLT 120
           FSQIEGFIDEHKEEAYEGIGKQVLAPFSCSSNQIARTWKMEKLFDHLNEVAAKMYEFNLT
Sbjct: 61  FSQIEGFIDEHKEEAYEGIGKQVLAPFSCSSNQIARTWKMEKLFDHLNEVAAKMYEFNLT 120

Query: 121 ERHTGAIKTETTNSFLTATEVSTRLMKPSWKVLYPLTNAPKFYQDERYRKILNDFKNPTL 180
           ERHTGAIKTETTNSFLTATEVSTRLMKPSWKVLYPLTNAPKFYQDERYRKILNDFKNPTL
Sbjct: 121 ERHTGAIKTETTNSFLTATEVSTRLMKPSWKVLYPLTNAPKFYQDERYRKILNDFKNPTL 180

Query: 181 GFFHIVGEAGIGKSTLAKFIYNDPEVEGMFPSRLWVCVKEEFDTQRLMKEILNFSYSPAT 240
           GFFHIVGEAGIGKSTLAKFIYNDPEVEGMFPSRLWVCVKEEFDTQRLMKEILNFSYSPAT
Sbjct: 181 GFFHIVGEAGIGKSTLAKFIYNDPEVEGMFPSRLWVCVKEEFDTQRLMKEILNFSYSPAT 240

Query: 241 CDNLTTKLCPTDQYLRERTFLLVFQDLSIKNLDNCSLFTSLLMMGKPGSKIIVTTQNEEI 300
           CDNLTTKLCPTDQYLRERTFLLVFQDLSIKNLDNCSLFTSLLMMGKPGSKIIVTTQNEEI
Sbjct: 241 CDNLTTKLCPTDQYLRERTFLLVFQDLSIKNLDNCSLFTSLLMMGKPGSKIIVTTQNEEI 300

Query: 301 ANAIELTMIYKVGQQSEQNRSQTALDTVTKETANVNNADQFVQANPLGKIDQSIPSQTIF 360
           ANAIELTMIYKVGQQSEQNRSQTALDTVTKETANVNNADQFVQANPLGKIDQSIPSQTIF
Sbjct: 301 ANAIELTMIYKVGQQSEQNRSQTALDTVTKETANVNNADQFVQANPLGKIDQSIPSQTIF 360

Query: 361 KVKRLSEKDSLSLFKDYASTYEGNEKDIMKTLKKCNGIPLAIKCLGSMLSLGPPATKWME 420
           KVKRLSEKDSLSLFKDYASTYEGNEKDIMKTLKKCNGIPLAIKCLGSMLSLGPPATKWME
Sbjct: 361 KVKRLSEKDSLSLFKDYASTYEGNEKDIMKTLKKCNGIPLAIKCLGSMLSLGPPATKWME 420

Query: 421 DNERQKGDNESSSTFSILKLCYNEMPSHLKRCFLYCSQLPNDSILSSNDVIQLWMANGLL 480
           DNERQKGDNESSSTFSILKLCYNEMPSHLKRCFLYCSQLPNDSILSSNDVIQLWMANGLL
Sbjct: 421 DNERQKGDNESSSTFSILKLCYNEMPSHLKRCFLYCSQLPNDSILSSNDVIQLWMANGLL 480

Query: 481 RSRQENYLSLEDIGEIYFKELCSRCFLQDVEEYGLGYWFKMHPLIRELARLVQKRTKDLI 540
           RSRQENYLSLEDIGEIYFKELCSRCFLQDVEEYGLGYWFKMHPLIRELARLVQKRTKDLI
Sbjct: 481 RSRQENYLSLEDIGEIYFKELCSRCFLQDVEEYGLGYWFKMHPLIRELARLVQKRTKDLI 540

Query: 541 SIKPVTNVTSIAFPVRDEVPSSSFLAEKCISKFQHLRLLYLGHTDLQEIPNTIETLNHLT 600
           SIKPVTNVTSIAFPVRDEVPSSSFLAEKCISKFQHLRLLYLGHTDLQEIPNTIETLNHLT
Sbjct: 541 SIKPVTNVTSIAFPVRDEVPSSSFLAEKCISKFQHLRLLYLGHTDLQEIPNTIETLNHLT 600

Query: 601 YLDLQGNKNIKRLPNAICNLQHLQTLILASCSALEELPKDICKLSNLRYLWVTSNKLRLH 660
           YLDLQGNKNIKRLPNAICNLQHLQTLILASCSALEELPKDICKLSNLRYLWVTSNKLRLH
Sbjct: 601 YLDLQGNKNIKRLPNAICNLQHLQTLILASCSALEELPKDICKLSNLRYLWVTSNKLRLH 660

Query: 661 KNGVGTMTSLRFLAIGGCDKLQDLFERPSCLVRLETLMIYDCNSLQLLPNEMGSLISLQN 720
           KNGVGTMTSLRFLAIGGCDKLQDLFERPSCLVRLETLMIYDCNSLQLLPNEMGSLISLQN
Sbjct: 661 KNGVGTMTSLRFLAIGGCDKLQDLFERPSCLVRLETLMIYDCNSLQLLPNEMGSLISLQN 720

Query: 721 LVIWSCKQLTLKGLEKVDFSLQRFTIRELPEVNKLPEWLQRSTETLRVLEIIDCPIKVEE 780
           LVIWSCKQLTLKGLEKVDFSLQRFTIRELPEVNKLPEWLQRSTETLRVLEIIDCPIKVEE
Sbjct: 721 LVIWSCKQLTLKGLEKVDFSLQRFTIRELPEVNKLPEWLQRSTETLRVLEIIDCPIKVEE 780

Query: 781 EGIKIH 787
           EGIK++
Sbjct: 781 EGIKMY 786

BLAST of Cucsa.337190 vs. NCBI nr
Match: gi|659084959|ref|XP_008443166.1| (PREDICTED: putative disease resistance protein RGA4 [Cucumis melo])

HSP 1 Score: 904.0 bits (2335), Expect = 1.8e-259
Identity = 493/799 (61.70%), Postives = 602/799 (75.34%), Query Frame = 1

Query: 1   MAYCIYYRAENILSELKNLPNYPRRIEYTMLSLKSILMDAEEKQEQSRGLQNWLEELQNV 60
           MA  IY +AENIL ELK  P Y RR++YTMLSLK+ILMDAE K+E S  L +WL+ELQNV
Sbjct: 1   MACRIYNQAENILRELKKFPKYLRRMQYTMLSLKTILMDAE-KEEYSHHLNDWLQELQNV 60

Query: 61  FSQIEGFIDEH--KEEAYEGIGKQVLAPFSCSSNQIARTWKMEKLFDHLNEVAAKMYEFN 120
           FSQIEG + E   K +  E  GK V    S +S++I +T KM KL D L+E+A++MY+FN
Sbjct: 61  FSQIEGLLYEFNGKVKKQEATGKWVFV-LSFNSSRIEQTKKMMKLCDDLDEIASQMYDFN 120

Query: 121 LTERHTGAIKTETTNSFLTATEVSTRLMKPSWKVLYPLTNAPKFYQDERYRKILNDFKNP 180
           LT         ETT+SFL+ATEVSTRLMKPSW++LYPLTNAPK + D+RY   L+ FK  
Sbjct: 121 LTNM-------ETTHSFLSATEVSTRLMKPSWQLLYPLTNAPKVFHDKRYSNFLDHFKKS 180

Query: 181 TLGFFHIVGEAGIGKSTLAKFIYNDPEVEGMFPSRLWVCVKEEFDTQRLMKEILNFSYSP 240
           TLGFFHIVGE GIGK+TLAKF YNDPEV   FPSRLW+CVKEEFD +RL+KE+L+FS+  
Sbjct: 181 TLGFFHIVGEPGIGKTTLAKFFYNDPEVVKTFPSRLWICVKEEFDPRRLIKEMLDFSHCQ 240

Query: 241 ATCDNLTTK-LC-PTDQYLRERTFLLVFQDLSIKNLDNCSLFTSLLMMGKPGSKIIVTTQ 300
           AT DNLT K LC    Q+LR++TFLLVFQD+SIKNL +CS+F SLL MG PGSKIIVTTQ
Sbjct: 241 ATYDNLTEKQLCFAVQQFLRDKTFLLVFQDISIKNLGDCSIFKSLLGMGNPGSKIIVTTQ 300

Query: 301 NEEIANAIELTMIYKVGQQSEQNRSQTALDTVTKETANVNNADQFVQANPLGKIDQSIPS 360
           NEEIA AI L  + K       N SQ        E ++VNN + +           ++  
Sbjct: 301 NEEIAEAIRLGKLCK-------NESQEVPSPEATEPSDVNNDEAY---------KDNMTY 360

Query: 361 QTIFKVKRLSEKDSLSLFKDYASTYEGNEKDIMKTLK-------KCNGIPLAIKCLGSML 420
           Q IF+V+RLS+++SLSLFK +A T E  E  I    K       KC+G+PLAIKCLG +L
Sbjct: 361 QKIFEVERLSKENSLSLFKVHAFT-ETQEAQIPNLTKIQEVIEQKCHGVPLAIKCLGGLL 420

Query: 421 SLGPPATKW--MEDN--ERQKGDNESSSTFSILKLCYNEMPSHLKRCFLYCSQLPNDSIL 480
           S    A +W  + D   E ++G++ + S   +L+LCY+ MPSHL+RCFLYCSQL  D IL
Sbjct: 421 SKTSIA-EWNGVIDKLWEHEEGEDGNKSILPVLRLCYDRMPSHLQRCFLYCSQLTKDRIL 480

Query: 481 SSNDVIQLWMANGLLRSRQENYLSLEDIGEIYFKELCSRCFLQDVEEYGLGYWFKMHPLI 540
           SSNDVIQLW+A+ LL   +ENYLSLE IGE YFKELCSRCFLQ++EEYGLGYWFK+HPLI
Sbjct: 481 SSNDVIQLWIASDLLP--KENYLSLEKIGENYFKELCSRCFLQELEEYGLGYWFKLHPLI 540

Query: 541 RELARLVQKRTKDLISIKPVTNVTSIAFPVRDEVPSSSFLAEKCISKFQHLRLLYLGHTD 600
            +LAR++ ++      + PV N   IAF VRD+VP S+FLA  CI KF+++RLLYLG+ +
Sbjct: 541 EKLARILTQK-----QVFPVANTKCIAFTVRDKVPPSAFLANTCIDKFKYIRLLYLGNAN 600

Query: 601 LQEIPNTIETLNHLTYLDLQGNKNIKRLPNAICNLQHLQTLILASCSALEELPKDICKLS 660
           L+EIPN +E L  L YLDLQGNK +KRLPN+I NL++LQTLILASCSALEELP  I +L 
Sbjct: 601 LREIPNAVEKLVQLRYLDLQGNKRLKRLPNSIFNLKNLQTLILASCSALEELPNHIKQLI 660

Query: 661 NLRYLWVTSNKLRLHKNGVGTMTSLRFLAIGGCDKLQDLFERPSCLVRLETLMIYDCNSL 720
           NLRYLWVT+N LRLHKNGVGTMTSLRFLAIGGC+ LQDLFE+PSCLVRLETLMIYD  +L
Sbjct: 661 NLRYLWVTANNLRLHKNGVGTMTSLRFLAIGGCENLQDLFEQPSCLVRLETLMIYDSKTL 720

Query: 721 QLLPNEMGSLISLQNLVIWSCKQLTLKGLEKVDFSLQRFTIRELPEVNKLPEWLQRSTET 780
           +LLPNE+G+LISL+NLVIWSCKQLTL  L+ V+F L+RFTIRELP V KLPEW+QR TE+
Sbjct: 721 KLLPNEIGALISLKNLVIWSCKQLTLT-LKGVEFRLRRFTIRELPRVRKLPEWIQRFTES 764

Query: 781 LRVLEIIDCPIKVEEEGIK 785
           LRVLEIIDCPI+++++  K
Sbjct: 781 LRVLEIIDCPIELKDDEFK 764

BLAST of Cucsa.337190 vs. NCBI nr
Match: gi|778688885|ref|XP_004136756.2| (PREDICTED: putative disease resistance protein RGA4 [Cucumis sativus])

HSP 1 Score: 864.8 bits (2233), Expect = 1.2e-247
Identity = 480/801 (59.93%), Postives = 581/801 (72.53%), Query Frame = 1

Query: 1   MAYCIYYRAENILSELKNLPNYPRRIEYTMLSLKSILMDAEEKQEQSRGLQNWLEELQNV 60
           MA CIY +AENIL ELK  P Y RR++YTMLSLK+IL DAE K+E    L +WL++LQ+V
Sbjct: 32  MACCIYEQAENILIELKKFPMYLRRMQYTMLSLKTILKDAE-KEEYRHCLNDWLQKLQSV 91

Query: 61  FSQIEGFIDEHKEEA--YEGIGKQVLAPFSCSSNQIARTWKMEKLFDHLNEVAAKMYEFN 120
           F QIE  + E   E    E  GK V  P S + +QI +T KM KL D L+E+A+ MY FN
Sbjct: 92  FLQIEELLYESNREVKKQEATGKWVFLP-SFNFSQIDQTKKMMKLCDDLDEIASHMYGFN 151

Query: 121 LTERHTGAIKTETTNSFLTATEVSTRLMKPSWKVLYPLTNAPKFYQDERYRKILNDFKNP 180
           LT         ETT+SFL+ATEVSTRLMKPSW++LY LTNAPK +QD+RY   L+ FK  
Sbjct: 152 LTNM-------ETTHSFLSATEVSTRLMKPSWQLLYSLTNAPKVFQDKRYHNFLDHFKKS 211

Query: 181 TLGFFHIVGEAGIGKSTLAKFIYNDPEVEGMFPSRLWVCVKEEFDTQRLMKEILNFSYSP 240
           T G FHIVGE GIGK+TLAKF YN+  +   FPSRLW+CVKEEFD QRL+KE+L+FS+  
Sbjct: 212 THGLFHIVGEPGIGKTTLAKFFYNN--LVNTFPSRLWICVKEEFDPQRLIKEMLSFSHCQ 271

Query: 241 ATCDNLTTK-LC-PTDQYLRERTFLLVFQDLSIKNLDNCSLFTSLLMMGKPGSKIIVTTQ 300
            TCDNLT K LC    Q+LR++ FL+VFQD+SIKNL NCS+F SLL MG  GSKIIVTTQ
Sbjct: 272 VTCDNLTEKQLCFAVQQFLRDKKFLIVFQDISIKNLGNCSIFKSLLGMGNRGSKIIVTTQ 331

Query: 301 NEEIANAIELTMIYKVGQQSEQNRSQTALDTVTKETANVNNADQFVQANPLGKIDQSIPS 360
           NE+IA+A+ L  +YK       N SQ        + ++VN                ++  
Sbjct: 332 NEKIADAVGLKKLYK-------NESQVVPSPEATKPSDVNK--------------DNMKH 391

Query: 361 QTIFKVKRLSEKDSLSLFKDYASTYEGNEKDIMKTLK-------KCNGIPLAIKCLGSML 420
           QTIFKV+RLS+++SLSLFK +A T E  E  I    K       KC+G+PLAIKCLG +L
Sbjct: 392 QTIFKVERLSKENSLSLFKVHAFT-ETQEAQIPNLTKIQEVIEQKCHGVPLAIKCLGGLL 451

Query: 421 SLGPPAT------KWMEDNERQKGDNESSSTFSILKLCYNEMPSHLKRCFLYCSQLPNDS 480
           S    A       K  E  E + G+    S    L+LCY++MPSHL+RCFLYCSQL  D 
Sbjct: 452 SKTSIAEWNGVIDKLWEHEEEEDGNK---SILPTLRLCYDQMPSHLQRCFLYCSQLKKDR 511

Query: 481 ILSSNDVIQLWMANGLLRSRQENYLSLEDIGEIYFKELCSRCFLQDVEEYGLGYWFKMHP 540
           ILSSNDVIQLW+A+ LL   +ENYLSLE IGE YFKELCSRCFLQ++EEYG GYWFK+HP
Sbjct: 512 ILSSNDVIQLWIASDLLP--KENYLSLEKIGENYFKELCSRCFLQELEEYGFGYWFKLHP 571

Query: 541 LIRELARLVQKRTKDLISIKPVTNVTSIAFPVRDEVPSSSFLAEKCISKFQHLRLLYLGH 600
           LI +LARL+ ++      +  VT   SIAF +RD+VP S+FLA  CI KF++LRLL+LG+
Sbjct: 572 LIEKLARLLTQK-----QVFEVTKTQSIAFTIRDKVPPSAFLANACIDKFKYLRLLHLGN 631

Query: 601 TDLQEIPNTIETLNHLTYLDLQGNKNIKRLPNAICNLQHLQTLILASCSALEELPKDICK 660
            +LQ IP+ +E L  L YLDLQGNK IKRLPN+I  L++LQTLILASCSAL+ELP DI +
Sbjct: 632 ANLQGIPSAVENLVQLRYLDLQGNKKIKRLPNSIFKLKNLQTLILASCSALKELPNDIRQ 691

Query: 661 LSNLRYLWVTSNKLRLHKNGVGTMTSLRFLAIGGCDKLQDLFERPSCLVRLETLMIYDCN 720
           L+NLRYLWVT+N LRLHKNGVGTMTSLRFLAIGGC  LQDLF++PSCLVRLETLMIYDC 
Sbjct: 692 LTNLRYLWVTANNLRLHKNGVGTMTSLRFLAIGGCQNLQDLFKKPSCLVRLETLMIYDCK 751

Query: 721 SLQLLPNEMGSLISLQNLVIWSCKQLTLKGLEKVDFSLQRFTIRELPEVNKLPEWLQRST 780
           +L+ LPNE+GSLISL+NLVIWSCK+LTL  L+ V+F LQRFTIRELP V KLPEW QR T
Sbjct: 752 TLKSLPNEIGSLISLKNLVIWSCKKLTLT-LKGVEFRLQRFTIRELPIVKKLPEWTQRFT 788

Query: 781 ETLRVLEIIDCPIKVEEEGIK 785
           ETLRVLEIIDCPI+  ++ +K
Sbjct: 812 ETLRVLEIIDCPIEWNDDVLK 788

BLAST of Cucsa.337190 vs. NCBI nr
Match: gi|700204239|gb|KGN59372.1| (hypothetical protein Csa_3G814390 [Cucumis sativus])

HSP 1 Score: 864.8 bits (2233), Expect = 1.2e-247
Identity = 480/801 (59.93%), Postives = 581/801 (72.53%), Query Frame = 1

Query: 1   MAYCIYYRAENILSELKNLPNYPRRIEYTMLSLKSILMDAEEKQEQSRGLQNWLEELQNV 60
           MA CIY +AENIL ELK  P Y RR++YTMLSLK+IL DAE K+E    L +WL++LQ+V
Sbjct: 1   MACCIYEQAENILIELKKFPMYLRRMQYTMLSLKTILKDAE-KEEYRHCLNDWLQKLQSV 60

Query: 61  FSQIEGFIDEHKEEA--YEGIGKQVLAPFSCSSNQIARTWKMEKLFDHLNEVAAKMYEFN 120
           F QIE  + E   E    E  GK V  P S + +QI +T KM KL D L+E+A+ MY FN
Sbjct: 61  FLQIEELLYESNREVKKQEATGKWVFLP-SFNFSQIDQTKKMMKLCDDLDEIASHMYGFN 120

Query: 121 LTERHTGAIKTETTNSFLTATEVSTRLMKPSWKVLYPLTNAPKFYQDERYRKILNDFKNP 180
           LT         ETT+SFL+ATEVSTRLMKPSW++LY LTNAPK +QD+RY   L+ FK  
Sbjct: 121 LTNM-------ETTHSFLSATEVSTRLMKPSWQLLYSLTNAPKVFQDKRYHNFLDHFKKS 180

Query: 181 TLGFFHIVGEAGIGKSTLAKFIYNDPEVEGMFPSRLWVCVKEEFDTQRLMKEILNFSYSP 240
           T G FHIVGE GIGK+TLAKF YN+  +   FPSRLW+CVKEEFD QRL+KE+L+FS+  
Sbjct: 181 THGLFHIVGEPGIGKTTLAKFFYNN--LVNTFPSRLWICVKEEFDPQRLIKEMLSFSHCQ 240

Query: 241 ATCDNLTTK-LC-PTDQYLRERTFLLVFQDLSIKNLDNCSLFTSLLMMGKPGSKIIVTTQ 300
            TCDNLT K LC    Q+LR++ FL+VFQD+SIKNL NCS+F SLL MG  GSKIIVTTQ
Sbjct: 241 VTCDNLTEKQLCFAVQQFLRDKKFLIVFQDISIKNLGNCSIFKSLLGMGNRGSKIIVTTQ 300

Query: 301 NEEIANAIELTMIYKVGQQSEQNRSQTALDTVTKETANVNNADQFVQANPLGKIDQSIPS 360
           NE+IA+A+ L  +YK       N SQ        + ++VN                ++  
Sbjct: 301 NEKIADAVGLKKLYK-------NESQVVPSPEATKPSDVNK--------------DNMKH 360

Query: 361 QTIFKVKRLSEKDSLSLFKDYASTYEGNEKDIMKTLK-------KCNGIPLAIKCLGSML 420
           QTIFKV+RLS+++SLSLFK +A T E  E  I    K       KC+G+PLAIKCLG +L
Sbjct: 361 QTIFKVERLSKENSLSLFKVHAFT-ETQEAQIPNLTKIQEVIEQKCHGVPLAIKCLGGLL 420

Query: 421 SLGPPAT------KWMEDNERQKGDNESSSTFSILKLCYNEMPSHLKRCFLYCSQLPNDS 480
           S    A       K  E  E + G+    S    L+LCY++MPSHL+RCFLYCSQL  D 
Sbjct: 421 SKTSIAEWNGVIDKLWEHEEEEDGNK---SILPTLRLCYDQMPSHLQRCFLYCSQLKKDR 480

Query: 481 ILSSNDVIQLWMANGLLRSRQENYLSLEDIGEIYFKELCSRCFLQDVEEYGLGYWFKMHP 540
           ILSSNDVIQLW+A+ LL   +ENYLSLE IGE YFKELCSRCFLQ++EEYG GYWFK+HP
Sbjct: 481 ILSSNDVIQLWIASDLLP--KENYLSLEKIGENYFKELCSRCFLQELEEYGFGYWFKLHP 540

Query: 541 LIRELARLVQKRTKDLISIKPVTNVTSIAFPVRDEVPSSSFLAEKCISKFQHLRLLYLGH 600
           LI +LARL+ ++      +  VT   SIAF +RD+VP S+FLA  CI KF++LRLL+LG+
Sbjct: 541 LIEKLARLLTQK-----QVFEVTKTQSIAFTIRDKVPPSAFLANACIDKFKYLRLLHLGN 600

Query: 601 TDLQEIPNTIETLNHLTYLDLQGNKNIKRLPNAICNLQHLQTLILASCSALEELPKDICK 660
            +LQ IP+ +E L  L YLDLQGNK IKRLPN+I  L++LQTLILASCSAL+ELP DI +
Sbjct: 601 ANLQGIPSAVENLVQLRYLDLQGNKKIKRLPNSIFKLKNLQTLILASCSALKELPNDIRQ 660

Query: 661 LSNLRYLWVTSNKLRLHKNGVGTMTSLRFLAIGGCDKLQDLFERPSCLVRLETLMIYDCN 720
           L+NLRYLWVT+N LRLHKNGVGTMTSLRFLAIGGC  LQDLF++PSCLVRLETLMIYDC 
Sbjct: 661 LTNLRYLWVTANNLRLHKNGVGTMTSLRFLAIGGCQNLQDLFKKPSCLVRLETLMIYDCK 720

Query: 721 SLQLLPNEMGSLISLQNLVIWSCKQLTLKGLEKVDFSLQRFTIRELPEVNKLPEWLQRST 780
           +L+ LPNE+GSLISL+NLVIWSCK+LTL  L+ V+F LQRFTIRELP V KLPEW QR T
Sbjct: 721 TLKSLPNEIGSLISLKNLVIWSCKKLTLT-LKGVEFRLQRFTIRELPIVKKLPEWTQRFT 757

Query: 781 ETLRVLEIIDCPIKVEEEGIK 785
           ETLRVLEIIDCPI+  ++ +K
Sbjct: 781 ETLRVLEIIDCPIEWNDDVLK 757

BLAST of Cucsa.337190 vs. NCBI nr
Match: gi|700204242|gb|KGN59375.1| (hypothetical protein Csa_3G815400 [Cucumis sativus])

HSP 1 Score: 711.4 bits (1835), Expect = 1.7e-201
Identity = 419/867 (48.33%), Postives = 560/867 (64.59%), Query Frame = 1

Query: 1   MAYCIYYRAENILSELKNLPNYPRRIEYTMLSLKSILMDAEEKQEQSRGLQNWLEELQNV 60
           MA+CIY +AENIL++LK+ P Y +RIEY M SLK++L+DAEEKQEQ++ LQNWL+ELQNV
Sbjct: 1   MAHCIYTQAENILTKLKDSPPYQKRIEYAMSSLKAVLLDAEEKQEQNQRLQNWLKELQNV 60

Query: 61  FSQIEGFIDEHKEEAYE--GIGKQVLAPFSCSS----NQIARTWKMEKLFDHLNEVAAKM 120
           F Q+E  IDE K E ++   IGKQVLAPFSCS+    N++ +  K +++ D LN +A +M
Sbjct: 61  FYQVEDSIDEFKWEIFKQKDIGKQVLAPFSCSNQISANKLKQKCKRKQVCDELNNIATRM 120

Query: 121 YEFNLTERHTGAIKTETTNSFLTATEVSTRLMKPSWKVLYPLTNAPKFYQDERYRKILND 180
           YEF+L  +H  +I  ETT++F +A+E+S R +KPSW++LYPL +A + Y DE Y  ILN 
Sbjct: 121 YEFHLKVKHIDSISMETTHTFPSASEISIRHLKPSWQLLYPLIDASRTY-DEIYDGILNV 180

Query: 181 FKNPTLGFFHIVGEAGIGKSTLAKFIYNDPEVEGMFPSRLWVCVKEEFD----------- 240
           F N     FHIVGEAGIGKST+A+F+YN   V G + SR WVCV+E F+           
Sbjct: 181 F-NECTHVFHIVGEAGIGKSTVARFLYNHNNVVGKYTSRYWVCVEEGFNKHRLVKEVYSH 240

Query: 241 -----------TQRLMKEILNF-------------------SYSPATCDNLT------TK 300
                      T++L+ ++                        SP   + L       +K
Sbjct: 241 ADNKEICEDLTTEQLLSKVKRLLKVETFLLVFQDLSITNLKDRSPLVLNELLEMGQHGSK 300

Query: 301 LCPTDQ------YLRERTFLLVFQDLSIKNL--------DNCSLFTSLLMMGKPGSKIIV 360
           +  T Q      YL++R +    +  S +NL         N SL  +      PGS+I  
Sbjct: 301 IIVTTQTEEIANYLQDRGYKT--ERRSKENLANGDRVSAHNQSLTKNTQNQAVPGSQI-- 360

Query: 361 TTQNEEIANAIELTMI-YKVGQQSEQNRSQTALDTVTKETANVN---NADQFVQANPLGK 420
               EE A+AI   +  + +    +    +  +  VT++T N+    N D      P+ +
Sbjct: 361 ----EETADAIANKIYEHSLSLTEDAQNLEVPIPQVTEDT-NIGLGINPD----IPPIKQ 420

Query: 421 IDQSIPSQTIFKVKRLSEKDSLSLFKDYA----STYEGNE--KDIMKTLKKCNGIPLAIK 480
            D     QTIFK+++LS++ S  LFK+YA       E  E  K + + L+KC G+PLAIK
Sbjct: 421 DDTEY--QTIFKLEKLSKQSSSRLFKEYAFRNRQEVENPELTKIVDQLLEKCMGVPLAIK 480

Query: 481 CLGSMLSLGPPATKWMEDNERQK-GDNESSSTFSILKLCYNEMPSHLKRCFLYCSQLPND 540
           CLGS+LS      KW    E+    + + +    +L++CY++MPS LK CFL+CSQLPND
Sbjct: 481 CLGSLLSSETSIAKWKNIEEKLSLQEKKENGILHVLRVCYDQMPSQLKPCFLHCSQLPND 540

Query: 541 SILSSNDVIQLWMANGLLRSRQENYLSLEDIGEIYFKELCSRCFLQDVEEYGLGYWFKMH 600
            I+SSND+IQLWMANGLL S +E   ++E+IGE YF EL SR F+Q++EE+GLGYW K+H
Sbjct: 541 RIISSNDMIQLWMANGLLHSPEEKNSTMENIGEKYFMELWSRYFIQEIEEHGLGYWIKLH 600

Query: 601 PLIRELARLVQKRTKDLIS----IKPVTNVTSIAFPVRDEVPSSSFLAEKCISKFQHLRL 660
           PLI++LA  + +   +        K VT + SIAF  R+ V  ++ L EKCI K++ LRL
Sbjct: 601 PLIQKLAHKITQEQSEGSGGNNHPKEVTEIRSIAFQERNMVLPNASLTEKCIWKYKGLRL 660

Query: 661 LYLGHTDLQEIPNTIETLNHLTYLDLQGNKNIKRLPNAICNLQHLQTLILASCSALEELP 720
           LYL + DLQEIPN+I TL +L YLDL GN  IK LPN+ICNLQ LQTLIL SCSALE+LP
Sbjct: 661 LYLSNADLQEIPNSIGTLKYLRYLDLHGNTKIKHLPNSICNLQSLQTLILGSCSALEDLP 720

Query: 721 KDICKLSNLRYLWVTSNKLRLHKNGVGTMTSLRFLAIGGCDKLQDLFERPSCLVRLETLM 780
           KDI  L +LRYLWVT+NKLRL KNGVGTM SLRFLAIGGC+ L++LFERP CL  LETLM
Sbjct: 721 KDIRNLISLRYLWVTTNKLRLDKNGVGTMNSLRFLAIGGCNNLENLFERPDCLAGLETLM 780

Query: 781 IYDCNSLQLLPNEMGSLISLQNLVIWSCKQLTLKGLEKVDFSLQRFTIRELPEVNKLPEW 785
           IY+C +L+LLP+EM  L SLQNL+IWSCKQLTL  L++V+F LQRFTI+ELP V +LP+W
Sbjct: 781 IYNCTTLKLLPDEMRYLKSLQNLMIWSCKQLTL-NLKEVEFKLQRFTIKELPRVERLPQW 840