Cucsa.337160 (gene) Cucumber (Gy14) v1

NameCucsa.337160
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionPhytochrome kinase substrate 4, putative
Locationscaffold03356 : 304312 .. 305976 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGTCTTATTAATAGAGGGTTATCTGAGAAAACAGTCTTTGGCTACAACAACAATAATGATATGAAAGAGAAAAGAGAGACTGCTAATACTAATTCTATTCCATCTTATATTACTCATTCTGATCACCAACATCACCATTTTCTTGCTCGTTGCACCATTGTTGATGATTCTTCTGAGTTGAGTATCTTTGATGCCAAGAAGTACTTCAATGAAGTTTCAGCCAACAATAATATTAACAAGGTTTCTCCCATAATGAACATCGATTCGATGTCGATGTCGATGTCGGAGCAATGTATGGATCATTCAGTAGCATCGTCTCAAGATCAGGATTCAGAAAAGGATTATGACTTATCCAAGTCGAAGCCTTGGACTTGTGGTGTTGCGCACCCGTCGTCTGGGGTGGTTTCTAAATTTTCACCAACTACTGCCTCTACTATAGATGGTCATCATCGTCGGAGTTACCGAGCTCGATCTTTCCATTCTGCAACGCCAACTGCCTCGTCGGAGGCTAGTTGGAATAGCCAGACGGGGTTGCTCTCGAACCCTCCTGGAGCAATCTCAGTGTCAGTTTTGCGTGGGGATTCTGATCATCATTCTGGGAGGAAAACTCGAAAGCCTCCTTCGTCATCCTCTTTGGCAGCAAGATGGATTTTTAGAAGCTCTAAATGCCCTTGTACTGGGAAGAAGTCGGTTCAAGTTCAGGAATCTAAAGTGGTACTAGACCCAAAAACAAGTCCTCCTTATATCAACAACACAACCGTTGGTGGCCATTCCCAATCTCAATCAGAGTCTCCGCCATCTGAAAAAACCTCTGGAGAAAACACAATGCTATTGCAACATCCAGACAAGGACGTTGTTTGGGGTAGTCAAAGACGTTTTCCTCCAAATTTGCTTCTTCAAGGACACACAACGCAACGAGTAATAGCCTCCACGGGCTTCACTTTTCCCATCTTGAAAAACAACAATAACAACAATGGAGATATTCCAACCAGAGCTATAGGTCATGTTTTAATTGAAGACCCTCCTCGAGATTCTCTAGAAGTTTTCAACCCAAGCAGTGCTAGAGATAGTGGCAACGGTGGTTGTAGTAGTCTAAAATCACGAATTCTAGCCAGTGTAGCAGCATCTGGCGGCAACGCCACCATTGTGAACGACATCGATGATGTAGCAAGTGATGCTAGCTCAGACTTATTTGAAATCGAAAGTTTTTCCACTCAAACTGCCAGCACAACCACGGCCTCATACCCTGCCATGTTCCACCGACGAGACTCAATGGAACTAGAAGCAAGAAGACTAGGGTTAACCTCTGCAGCCACACGTCGCAGCCTCGACGAGCCAATGACACCTTCAACTGATTGGTATGAACCAAGCGAGGCAAGCATCGACTGGAGCGTTACCACAGCCGAGGGCTTCGATCGGGCGAGCATTGCCAACATGTCAGAGGCCGAGGAGTCCTGGACTGAGAAAAACAACAACAACAACAATAACAACAGACGAAGATCATCATCGGGAAATGGGTTGTTAAGTTGTCGGTCAGAGAAGGCGGTGAGTGTAGGCCCACAGCCGGTGATGAAGCATGTGAGTAGTAGGCCACCATTGGGAAAGAAGCCACCACTAGCAAGGTCTAATTCAGCTCACCTCTCCCTTACTTTTGCTGCCTGA

mRNA sequence

ATGAGTCTTATTAATAGAGGGTTATCTGAGAAAACAGTCTTTGGCTACAACAACAATAATGATATGAAAGAGAAAAGAGAGACTGCTAATACTAATTCTATTCCATCTTATATTACTCATTCTGATCACCAACATCACCATTTTCTTGCTCGTTGCACCATTGTTGATGATTCTTCTGAGTTGAGTATCTTTGATGCCAAGAAGTACTTCAATGAAGTTTCAGCCAACAATAATATTAACAAGGTTTCTCCCATAATGAACATCGATTCGATGTCGATGTCGATGTCGGAGCAATGTATGGATCATTCAGTAGCATCGTCTCAAGATCAGGATTCAGAAAAGGATTATGACTTATCCAAGTCGAAGCCTTGGACTTGTGGTGTTGCGCACCCGTCGTCTGGGGTGGTTTCTAAATTTTCACCAACTACTGCCTCTACTATAGATGGTCATCATCGTCGGAGTTACCGAGCTCGATCTTTCCATTCTGCAACGCCAACTGCCTCGTCGGAGGCTAGTTGGAATAGCCAGACGGGGTTGCTCTCGAACCCTCCTGGAGCAATCTCAGTGTCAGTTTTGCGTGGGGATTCTGATCATCATTCTGGGAGGAAAACTCGAAAGCCTCCTTCGTCATCCTCTTTGGCAGCAAGATGGATTTTTAGAAGCTCTAAATGCCCTTGTACTGGGAAGAAGTCGGTTCAAGTTCAGGAATCTAAAGTGGTACTAGACCCAAAAACAAGTCCTCCTTATATCAACAACACAACCGTTGGTGGCCATTCCCAATCTCAATCAGAGTCTCCGCCATCTGAAAAAACCTCTGGAGAAAACACAATGCTATTGCAACATCCAGACAAGGACGTTGTTTGGGGTAGTCAAAGACGTTTTCCTCCAAATTTGCTTCTTCAAGGACACACAACGCAACGAGTAATAGCCTCCACGGGCTTCACTTTTCCCATCTTGAAAAACAACAATAACAACAATGGAGATATTCCAACCAGAGCTATAGGTCATGTTTTAATTGAAGACCCTCCTCGAGATTCTCTAGAAGTTTTCAACCCAAGCAGTGCTAGAGATAGTGGCAACGGTGGTTGTAGTAGTCTAAAATCACGAATTCTAGCCAGTGTAGCAGCATCTGGCGGCAACGCCACCATTGTGAACGACATCGATGATGTAGCAAGTGATGCTAGCTCAGACTTATTTGAAATCGAAAGTTTTTCCACTCAAACTGCCAGCACAACCACGGCCTCATACCCTGCCATGTTCCACCGACGAGACTCAATGGAACTAGAAGCAAGAAGACTAGGGTTAACCTCTGCAGCCACACGTCGCAGCCTCGACGAGCCAATGACACCTTCAACTGATTGGTATGAACCAAGCGAGGCAAGCATCGACTGGAGCGTTACCACAGCCGAGGGCTTCGATCGGGCGAGCATTGCCAACATGTCAGAGGCCGAGGAGTCCTGGACTGAGAAAaacaacaacaacaacaataacaacaGACGAAGATCATCATCGGGAAATGGGTTGTTAAGTTGTCGGTCAGAGAAGGCGGTGAGTGTAGGCCCACAGCCGGTGATGAAGCATGTGAGTAGTAGGCCACCATTGGGAAAGAAGCCACCACTAGCAAGGTCTAATTCAGCTCACCTCTCCCTTACTTTTGCTGCCTGA

Coding sequence (CDS)

ATGAGTCTTATTAATAGAGGGTTATCTGAGAAAACAGTCTTTGGCTACAACAACAATAATGATATGAAAGAGAAAAGAGAGACTGCTAATACTAATTCTATTCCATCTTATATTACTCATTCTGATCACCAACATCACCATTTTCTTGCTCGTTGCACCATTGTTGATGATTCTTCTGAGTTGAGTATCTTTGATGCCAAGAAGTACTTCAATGAAGTTTCAGCCAACAATAATATTAACAAGGTTTCTCCCATAATGAACATCGATTCGATGTCGATGTCGATGTCGGAGCAATGTATGGATCATTCAGTAGCATCGTCTCAAGATCAGGATTCAGAAAAGGATTATGACTTATCCAAGTCGAAGCCTTGGACTTGTGGTGTTGCGCACCCGTCGTCTGGGGTGGTTTCTAAATTTTCACCAACTACTGCCTCTACTATAGATGGTCATCATCGTCGGAGTTACCGAGCTCGATCTTTCCATTCTGCAACGCCAACTGCCTCGTCGGAGGCTAGTTGGAATAGCCAGACGGGGTTGCTCTCGAACCCTCCTGGAGCAATCTCAGTGTCAGTTTTGCGTGGGGATTCTGATCATCATTCTGGGAGGAAAACTCGAAAGCCTCCTTCGTCATCCTCTTTGGCAGCAAGATGGATTTTTAGAAGCTCTAAATGCCCTTGTACTGGGAAGAAGTCGGTTCAAGTTCAGGAATCTAAAGTGGTACTAGACCCAAAAACAAGTCCTCCTTATATCAACAACACAACCGTTGGTGGCCATTCCCAATCTCAATCAGAGTCTCCGCCATCTGAAAAAACCTCTGGAGAAAACACAATGCTATTGCAACATCCAGACAAGGACGTTGTTTGGGGTAGTCAAAGACGTTTTCCTCCAAATTTGCTTCTTCAAGGACACACAACGCAACGAGTAATAGCCTCCACGGGCTTCACTTTTCCCATCTTGAAAAACAACAATAACAACAATGGAGATATTCCAACCAGAGCTATAGGTCATGTTTTAATTGAAGACCCTCCTCGAGATTCTCTAGAAGTTTTCAACCCAAGCAGTGCTAGAGATAGTGGCAACGGTGGTTGTAGTAGTCTAAAATCACGAATTCTAGCCAGTGTAGCAGCATCTGGCGGCAACGCCACCATTGTGAACGACATCGATGATGTAGCAAGTGATGCTAGCTCAGACTTATTTGAAATCGAAAGTTTTTCCACTCAAACTGCCAGCACAACCACGGCCTCATACCCTGCCATGTTCCACCGACGAGACTCAATGGAACTAGAAGCAAGAAGACTAGGGTTAACCTCTGCAGCCACACGTCGCAGCCTCGACGAGCCAATGACACCTTCAACTGATTGGTATGAACCAAGCGAGGCAAGCATCGACTGGAGCGTTACCACAGCCGAGGGCTTCGATCGGGCGAGCATTGCCAACATGTCAGAGGCCGAGGAGTCCTGGACTGAGAAAAACAACAACAACAACAATAACAACAGACGAAGATCATCATCGGGAAATGGGTTGTTAAGTTGTCGGTCAGAGAAGGCGGTGAGTGTAGGCCCACAGCCGGTGATGAAGCATGTGAGTAGTAGGCCACCATTGGGAAAGAAGCCACCACTAGCAAGGTCTAATTCAGCTCACCTCTCCCTTACTTTTGCTGCCTGA

Protein sequence

MSLINRGLSEKTVFGYNNNNDMKEKRETANTNSIPSYITHSDHQHHHFLARCTIVDDSSELSIFDAKKYFNEVSANNNINKVSPIMNIDSMSMSMSEQCMDHSVASSQDQDSEKDYDLSKSKPWTCGVAHPSSGVVSKFSPTTASTIDGHHRRSYRARSFHSATPTASSEASWNSQTGLLSNPPGAISVSVLRGDSDHHSGRKTRKPPSSSSLAARWIFRSSKCPCTGKKSVQVQESKVVLDPKTSPPYINNTTVGGHSQSQSESPPSEKTSGENTMLLQHPDKDVVWGSQRRFPPNLLLQGHTTQRVIASTGFTFPILKNNNNNNGDIPTRAIGHVLIEDPPRDSLEVFNPSSARDSGNGGCSSLKSRILASVAASGGNATIVNDIDDVASDASSDLFEIESFSTQTASTTTASYPAMFHRRDSMELEARRLGLTSAATRRSLDEPMTPSTDWYEPSEASIDWSVTTAEGFDRASIANMSEAEESWTEKNNNNNNNNRRRSSSGNGLLSCRSEKAVSVGPQPVMKHVSSRPPLGKKPPLARSNSAHLSLTFAA*
BLAST of Cucsa.337160 vs. Swiss-Prot
Match: PKS4_ARATH (Protein PHYTOCHROME KINASE SUBSTRATE 4 OS=Arabidopsis thaliana GN=PKS4 PE=1 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 4.3e-26
Identity = 131/403 (32.51%), Postives = 165/403 (40.94%), Query Frame = 1

Query: 166 TASSEASWNSQTGLLSNPPGAISVSVLRGDSDHHSGRKTRKPPSSSSLAARWIFRSSKCP 225
           TASSEASWNSQTGLLSN          R  SD    R ++K P       RW FR   CP
Sbjct: 100 TASSEASWNSQTGLLSNKN--------RQGSDRDGRRSSKKGP-------RWFFRRRACP 159

Query: 226 CTGKKSVQVQESKV-VLDPKT-SPPYINNTTVGGHSQSQSESPPSEKTSGENTMLLQHPD 285
           C+  KSVQVQESK  +  PKT S   ++N  V  H Q+ S   P   T   NT+      
Sbjct: 160 CSSSKSVQVQESKPRIAVPKTGSDRIVSNRIVHSH-QTISSPEPIRLTIPSNTVT---RS 219

Query: 286 KDVVWGSQRRFPPNLLLQGHTTQRVIASTGFTFPILKNNNNNNGDIPTRAIGH---VLIE 345
            D     + R P                + F+FP L   +  + +     + H   V IE
Sbjct: 220 IDYTANKEARAP---------------VSNFSFPTLNETSQLSENPKNPVLNHIKPVRIE 279

Query: 346 DPPRDSLEVFNPSSARDSGNGGCSSLKSRILASVAASGGNATIVNDIDDVASDASSDLFE 405
                   V NP+S +                          ++ D ++  SDASSDLFE
Sbjct: 280 PALLPIKPVLNPTSPKG-------------------------VIID-EEATSDASSDLFE 339

Query: 406 IESFSTQTASTTTASYPAMFHRRDSMELEARRLGLTSAATRRSLDEPMTPSTDWYEPSEA 465
           IESFSTQTA+   A  P +   RDSME      G                    YEPSEA
Sbjct: 340 IESFSTQTAARPWA--PPV---RDSMEETVSEYG--------------------YEPSEA 399

Query: 466 SIDWSVTTAE-------GFDRASIANMSEAEESWTEKNNNNNNNNRRRSSSGNGLLSCRS 525
           S+ WSV TAE        F R ++++ S A   + +K                GLL+C  
Sbjct: 400 SVTWSVMTAEPASAVAANFSRIALSSSSTAFSGYDKKR--------------TGLLNCHC 401

Query: 526 EKAVSVG-----PQPVMKHVSSRPPLGKKPPLARSNSAHLSLT 552
           EKAV V       QPV          GK   L  + S+ LS+T
Sbjct: 460 EKAVMVNGDKRLVQPVKSVGVQNDVAGK--VLCNNGSSKLSVT 401

BLAST of Cucsa.337160 vs. TrEMBL
Match: A0A0A0LBH5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G814370 PE=4 SV=1)

HSP 1 Score: 1115.9 bits (2885), Expect = 0.0e+00
Identity = 554/554 (100.00%), Postives = 554/554 (100.00%), Query Frame = 1

Query: 1   MSLINRGLSEKTVFGYNNNNDMKEKRETANTNSIPSYITHSDHQHHHFLARCTIVDDSSE 60
           MSLINRGLSEKTVFGYNNNNDMKEKRETANTNSIPSYITHSDHQHHHFLARCTIVDDSSE
Sbjct: 9   MSLINRGLSEKTVFGYNNNNDMKEKRETANTNSIPSYITHSDHQHHHFLARCTIVDDSSE 68

Query: 61  LSIFDAKKYFNEVSANNNINKVSPIMNIDSMSMSMSEQCMDHSVASSQDQDSEKDYDLSK 120
           LSIFDAKKYFNEVSANNNINKVSPIMNIDSMSMSMSEQCMDHSVASSQDQDSEKDYDLSK
Sbjct: 69  LSIFDAKKYFNEVSANNNINKVSPIMNIDSMSMSMSEQCMDHSVASSQDQDSEKDYDLSK 128

Query: 121 SKPWTCGVAHPSSGVVSKFSPTTASTIDGHHRRSYRARSFHSATPTASSEASWNSQTGLL 180
           SKPWTCGVAHPSSGVVSKFSPTTASTIDGHHRRSYRARSFHSATPTASSEASWNSQTGLL
Sbjct: 129 SKPWTCGVAHPSSGVVSKFSPTTASTIDGHHRRSYRARSFHSATPTASSEASWNSQTGLL 188

Query: 181 SNPPGAISVSVLRGDSDHHSGRKTRKPPSSSSLAARWIFRSSKCPCTGKKSVQVQESKVV 240
           SNPPGAISVSVLRGDSDHHSGRKTRKPPSSSSLAARWIFRSSKCPCTGKKSVQVQESKVV
Sbjct: 189 SNPPGAISVSVLRGDSDHHSGRKTRKPPSSSSLAARWIFRSSKCPCTGKKSVQVQESKVV 248

Query: 241 LDPKTSPPYINNTTVGGHSQSQSESPPSEKTSGENTMLLQHPDKDVVWGSQRRFPPNLLL 300
           LDPKTSPPYINNTTVGGHSQSQSESPPSEKTSGENTMLLQHPDKDVVWGSQRRFPPNLLL
Sbjct: 249 LDPKTSPPYINNTTVGGHSQSQSESPPSEKTSGENTMLLQHPDKDVVWGSQRRFPPNLLL 308

Query: 301 QGHTTQRVIASTGFTFPILKNNNNNNGDIPTRAIGHVLIEDPPRDSLEVFNPSSARDSGN 360
           QGHTTQRVIASTGFTFPILKNNNNNNGDIPTRAIGHVLIEDPPRDSLEVFNPSSARDSGN
Sbjct: 309 QGHTTQRVIASTGFTFPILKNNNNNNGDIPTRAIGHVLIEDPPRDSLEVFNPSSARDSGN 368

Query: 361 GGCSSLKSRILASVAASGGNATIVNDIDDVASDASSDLFEIESFSTQTASTTTASYPAMF 420
           GGCSSLKSRILASVAASGGNATIVNDIDDVASDASSDLFEIESFSTQTASTTTASYPAMF
Sbjct: 369 GGCSSLKSRILASVAASGGNATIVNDIDDVASDASSDLFEIESFSTQTASTTTASYPAMF 428

Query: 421 HRRDSMELEARRLGLTSAATRRSLDEPMTPSTDWYEPSEASIDWSVTTAEGFDRASIANM 480
           HRRDSMELEARRLGLTSAATRRSLDEPMTPSTDWYEPSEASIDWSVTTAEGFDRASIANM
Sbjct: 429 HRRDSMELEARRLGLTSAATRRSLDEPMTPSTDWYEPSEASIDWSVTTAEGFDRASIANM 488

Query: 481 SEAEESWTEKNNNNNNNNRRRSSSGNGLLSCRSEKAVSVGPQPVMKHVSSRPPLGKKPPL 540
           SEAEESWTEKNNNNNNNNRRRSSSGNGLLSCRSEKAVSVGPQPVMKHVSSRPPLGKKPPL
Sbjct: 489 SEAEESWTEKNNNNNNNNRRRSSSGNGLLSCRSEKAVSVGPQPVMKHVSSRPPLGKKPPL 548

Query: 541 ARSNSAHLSLTFAA 555
           ARSNSAHLSLTFAA
Sbjct: 549 ARSNSAHLSLTFAA 562

BLAST of Cucsa.337160 vs. TrEMBL
Match: A0A067KR89_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_05972 PE=4 SV=1)

HSP 1 Score: 303.5 bits (776), Expect = 5.2e-79
Identity = 209/467 (44.75%), Postives = 264/467 (56.53%), Query Frame = 1

Query: 142 TTASTIDGHHRRSYRARSFHSATPTASSEASWNSQTGLLSNPPGAISVSVLRGDSDHHSG 201
           + +S++DG+ R +YR RSFH ATPTASSEASWNSQTGLLSNPPGAI+VS+    S H+  
Sbjct: 113 SASSSVDGYGR-NYRTRSFH-ATPTASSEASWNSQTGLLSNPPGAIAVSIRNPASGHNDE 172

Query: 202 RKTRKPPSSSSLAARWIFRSSKCPCTGKKSVQVQESKVVLDPKTSPPYINNTTVGGHSQS 261
           +K R        A++W+    +CPC+GKKSVQV+E   + +P+T P  +N+      +  
Sbjct: 173 KKKR-------FASKWLL-GRRCPCSGKKSVQVEEK--LSEPRT-PSRLNHKFKEDRNLK 232

Query: 262 QSESPPS-EKTSGENTMLLQHPD-----KDVVWGSQRRFPPNL----LLQGHTTQRVIAS 321
           +  +P S EK        LQ  +       ++     RFP  +    +L   T      +
Sbjct: 233 RQITPDSVEKCCAVTDHWLQRQEVIPISHTIISADNNRFPSGISHHRVLASSTRPFTDGT 292

Query: 322 TGFTFPILKNN-------------------NNNNGDIPTRAIGHVLIEDPPRDSLEVFNP 381
            GF+FPIL                      + NN   PT     + +EDPPRDSLEVF P
Sbjct: 293 NGFSFPILNQTTLSSLSPMKLALNGLPSPTSYNNPPPPTP----LPLEDPPRDSLEVFQP 352

Query: 382 SSARDSGNGGCSSLKSRILASVAASGGNATIVNDIDDVASDASSDLFEIESFSTQTASTT 441
           S    S   G + L+ R   +  AS  +   + D DD+ASDASSDLFEIESFSTQT S  
Sbjct: 353 SDDPVSTKTGAAELQRRQSFTFQASPKSRVAITD-DDIASDASSDLFEIESFSTQTTS-- 412

Query: 442 TASYPAMFHRRDSME----LEARRLG-LTSAATRRSLDEPMTPSTDWYEPSEASIDWSVT 501
              YP   HRRDSM+      ARR G   S   R+SLD  + P+  +YEPSEASIDWSVT
Sbjct: 413 ---YPMYNHRRDSMDEASIFNARRSGGSNSGYCRQSLDALIAPTECYYEPSEASIDWSVT 472

Query: 502 TAEGFDRASIANMSEAEESW------TEKNNNNNNNNRRRSSSGN-GLLSCRSEKAVSVG 554
           TAEGFD+AS+ N SEAEE+       +EK++ +    +RR   GN GLLSCR EKAVSVG
Sbjct: 473 TAEGFDKASVTNFSEAEETTIMRRHESEKSSGSGGCGKRRGGEGNGGLLSCRCEKAVSVG 532

BLAST of Cucsa.337160 vs. TrEMBL
Match: A0A067KR89_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_05972 PE=4 SV=1)

HSP 1 Score: 37.7 bits (86), Expect = 5.3e+01
Identity = 25/81 (30.86%), Postives = 43/81 (53.09%), Query Frame = 1

Query: 36  SYITHSDHQHHHFLARCTIVDDSSELSIFDAKKYFNEVSANNN---INKVSP--IMNIDS 95
           S+ +H  HQ     ++     + SELSIFDA+KYFNE S + +     +VSP  ++N++ 
Sbjct: 43  SFSSHLKHQQQQLNSQA----EDSELSIFDAQKYFNENSNSGDSRMSKRVSPVNVINLER 102

Query: 96  MSMSMSEQCMDHSVASSQDQD 112
           +S +     +    ++S   D
Sbjct: 103 ISQTCDFTSLPRFSSASSSVD 119


HSP 2 Score: 278.9 bits (712), Expect = 1.4e-71
Identity = 215/500 (43.00%), Postives = 270/500 (54.00%), Query Frame = 1

Query: 127 GVAHPSSGVVSKFSPTTASTIDGHHRRSYRARSFHSATPTASSEASWNSQTGLLSNPPGA 186
           G  H  S  V++ S  + S+++G+ R +YRARSFH ATPTASSEASWNSQTGLL NPPG 
Sbjct: 87  GAEHCESQFVTRTS--SVSSVEGYGR-NYRARSFH-ATPTASSEASWNSQTGLLCNPPGT 146

Query: 187 ISVSV--LRGDSDHHSGRKTRKPPSSSSLAARWIFRSSKCPCTGKKSVQVQESKVVLDPK 246
           I VSV  +  +  + SGRK             W F   KCPC+GKK+V++QE   V +P+
Sbjct: 147 IGVSVRSIGSEKKNVSGRK-------------WFF-GRKCPCSGKKAVEIQEK--VSEPR 206

Query: 247 TSPPYINNTTVGGHSQSQSESPPSEKTSG--------ENTMLLQHP--------DKDVVW 306
            SP  +N      H+       P+E++SG        ++   L  P        D+  + 
Sbjct: 207 -SPIRLN------HNLGSDPKRPTERSSGNTLQKVSKKSAKSLDSPHGHGRDWADRHDLI 266

Query: 307 GSQRRFPPNLLLQGHT-TQRVIAS-TGFTFPILKNNNNNNGDIPTRAIGHVLIEDPPRDS 366
            S  +FPP   L  +   +RV+AS TGF+FPIL         + T A   + +EDPPRDS
Sbjct: 267 PSPPKFPPATHLPSNQLARRVLASGTGFSFPILSQAPPVKL-VFTSARNPIPLEDPPRDS 326

Query: 367 LEVFNPSSARDSGNGGCSSLKSRILASVAASGGNATIVNDI----------DDVASDASS 426
           LE+F PS    S      S+  RI AS  A+    +               DDVASDASS
Sbjct: 327 LEIFRPSDDCISRKS-TDSIAPRIFASNPAAAERRSFTFSASPKSRGTAMDDDVASDASS 386

Query: 427 DLFEIESFSTQTASTTTAS--YPAMFHRRDSMELEARRLGLTSAATRRSLDEPMTPS--- 486
           DLFEIESF+TQT +TTT +  YP M+HRRDS++  +RR G      RRSLDEP TPS   
Sbjct: 387 DLFEIESFTTQTTTTTTTATNYP-MYHRRDSLDDASRRFGYC----RRSLDEPATPSVAP 446

Query: 487 TDWYEPSEASIDWSVTTAEGFDRASIANMSEAEESWTE-----------------KNNNN 546
           T+ YEPSEASIDWSVTTAEGFDR S+ N S +     +                  +N+ 
Sbjct: 447 TECYEPSEASIDWSVTTAEGFDRGSVTNFSMSASDIVDDVTLMRQELQRLGNGGFNDNDG 506

Query: 547 NNNNRRRSSSGNG-LLSCRSEKAVSVGPQP--------------------VMKHVSSRPP 554
               RRR   GNG L+SCR EKAVSVGP P                      +HVSSR  
Sbjct: 507 GGGGRRR---GNGMLMSCRCEKAVSVGPGPTPVKEGQWGRGGGPGYIGSSTSRHVSSRVG 549

BLAST of Cucsa.337160 vs. TrEMBL
Match: F6HMU6_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_08s0056g00720 PE=4 SV=1)

HSP 1 Score: 32.3 bits (72), Expect = 2.2e+03
Identity = 15/33 (45.45%), Postives = 23/33 (69.70%), Query Frame = 1

Query: 55 VDDSSELSIFDAKKYFNEVSAN--NNINKVSPI 86
          + + SE+SIFDA+KYFNE S +      +V+P+
Sbjct: 54 IAEDSEISIFDAQKYFNESSEHQQRESKRVAPV 86


HSP 2 Score: 273.9 bits (699), Expect = 4.4e-70
Identity = 207/479 (43.22%), Postives = 264/479 (55.11%), Query Frame = 1

Query: 136 VSKFSPTTASTIDGHHRRSYRARSFHSATPTASSEASWNSQTGLLSNPPGAISVSVLRGD 195
           + +FS + +S++DG+ R +YRARSFH ATPTASSEASWNSQTGLLSNPPGAI+VS+    
Sbjct: 98  IPRFS-SVSSSVDGYGR-NYRARSFH-ATPTASSEASWNSQTGLLSNPPGAIAVSM---- 157

Query: 196 SDHHSGRKTRKPPSS----SSLAARWIFRSSKCPCTGKKSVQVQESKVVLDPKTSPPYIN 255
                    R PP S        A+W+    KCPC GKKS+QV+E   + +P T    ++
Sbjct: 158 ---------RNPPPSDEKKKGSKAKWLL-GRKCPCAGKKSIQVEEK--LSEPITPSRLVD 217

Query: 256 NTTVGGHSQSQSESP-PSEKTSGENTMLLQH---PDKDVVWGSQRRFPPNLLLQGH---- 315
           + +       Q + P  SEK+S     L +H   P+   +     RFP  L    H    
Sbjct: 218 HKSKDTDRNLQRKIPDSSEKSSAATDWLQRHEVIPNSHRITADNNRFPLALSQHHHRVLA 277

Query: 316 TTQRVIAS----TGFTFPILKNNNNNNGDIPTRAIGHVL---------IEDPPRDSLEVF 375
           T+ R  +S    +GF+FPIL    N     P + + + L         +EDPPRDSLEVF
Sbjct: 278 TSARPFSSDGTTSGFSFPIL----NQTSLPPIKLVLNGLQSSTNYNPQLEDPPRDSLEVF 337

Query: 376 NPS-SARDSGNGGCSSLKSRILASVAASGGNATIVNDIDDVASDASSDLFEIESFSTQTA 435
            PS     + N     L  R   +  AS  +  IV D DD+ASDASSDLFEIESFSTQT 
Sbjct: 338 LPSDDPIPTNNKTTMDLHQRQSFTFPASPKSRMIVTD-DDIASDASSDLFEIESFSTQT- 397

Query: 436 STTTASYPAMFHRRDSME----LEARRLGLTSAATRRSLDEPMTPS---TDWYEPSEASI 495
            T+   +     RRDS++       +RLG  +   R+SLDE MTPS   T+ Y PSEASI
Sbjct: 398 -TSYQMHNNNHGRRDSLDDASVFNGKRLG-GNLYCRQSLDESMTPSIAPTECYAPSEASI 457

Query: 496 DWSVTTAEGFDRASIAN--MSEAEESWTEKNNNNNNNNRR-----------RSSSGNGLL 555
           DWSVTTAEGFDRAS+ N  M+ +E    E  +  ++ + R           R     GLL
Sbjct: 458 DWSVTTAEGFDRASVTNFSMTASEVDHQEMISMRHHESERTIGGGGGSGGKRRGGNRGLL 517

BLAST of Cucsa.337160 vs. TrEMBL
Match: B9S4J7_RICCO (Putative uncharacterized protein OS=Ricinus communis GN=RCOM_0809110 PE=4 SV=1)

HSP 1 Score: 35.4 bits (80), Expect = 2.6e+02
Identity = 19/39 (48.72%), Postives = 29/39 (74.36%), Query Frame = 1

Query: 57 DSSELSIFDAKKYFNEVSANNNI--NKVSP--IMNIDSM 92
          D  E+SIFDA+KYFNE S +++    +VSP  ++NI+S+
Sbjct: 60 DDPEISIFDAQKYFNESSGSDHRVGKRVSPVNVINIESI 98


HSP 2 Score: 272.3 bits (695), Expect = 1.3e-69
Identity = 219/564 (38.83%), Postives = 293/564 (51.95%), Query Frame = 1

Query: 33  SIPSYITHSDHQHHHFLARCTIVDDSSELSIFDAKKYFNEVSANNNINKVSPIMNIDSMS 92
           S  SY+   +HQ  H  A      D SE+ IF+A+KYFNE ++N NI+  + I    S S
Sbjct: 45  SFASYLIRPNHQDIHVNAGQA---DDSEICIFEARKYFNETNSNINIDSKAII----SSS 104

Query: 93  MSMSEQCMDHSVASSQDQDSEKDYDLSKSKPWTCGVAHPSSGVVSKFSPTTASTIDGHHR 152
            + + +    +V S+ + ++     +S+ +         SS V +    + +S++DG+ R
Sbjct: 105 NNNNNRVSPLNVTSNNNNNNNNPELISEHRI--------SSDVSALPRFSCSSSVDGYGR 164

Query: 153 RSYRARSFHSATPTASSEASWNSQTGLLSNPPGAISVSVLRGDSDHHSGRKTRKPPSSSS 212
            +YR RSF+ ATPTASSEASWNSQTGLL NPPGA+++S+            T +      
Sbjct: 165 -NYRPRSFN-ATPTASSEASWNSQTGLLQNPPGAVAISM--------RNPATEEKKRGGR 224

Query: 213 LAARWIFRSSKCPCTGKKSVQVQESKVVLDPKTSPPYINNTTVGGHSQSQSESPPSEKTS 272
            +++W     KCPC+GKKSVQV+E       K S    ++++V   S S     P+++  
Sbjct: 225 TSSKWRLFGRKCPCSGKKSVQVEE-------KAS----SSSSVASVSVSAEPKAPADRLY 284

Query: 273 GENTML---LQHP-----DKDVVWGSQRRFPPNLLLQGHTTQRV----------IASTGF 332
                +   L+ P       + +  + RR        G   QRV           A+ GF
Sbjct: 285 NNKRQIQKSLEIPVDRSFQSEEIITNIRRISAECRFTGLGQQRVNIVPPGRPYTDATPGF 344

Query: 333 TFPILKNNNNNNGDIPTRAIGHVLIEDPPRDSLEVFNPSSARDSGNGGCSSLKSRILASV 392
           TFPIL    N    +        L+EDPPR+SLE+F P   +++     S   +R   + 
Sbjct: 345 TFPIL----NQTASMKLVLNNAPLLEDPPRESLEIFQPPLDQET---AVSRNATRHSFTF 404

Query: 393 AASGGNATIVNDIDDVASDASSDLFEIESFSTQTASTTTASYPAMFHRRDSME----LEA 452
            AS G   +  D DDVASDASSDLFEIESFSTQ     T +YP M+HRRDS++      A
Sbjct: 405 PASPGRTRMTTD-DDVASDASSDLFEIESFSTQ-----TTTYP-MYHRRDSLDEAASFNA 464

Query: 453 RRLGLTSA------ATRRSLDEPMTPS---TDWYEPSEASIDWSVTTAEGFDRASIANMS 512
           RRL   +        TR SLDE +TPS   T+ YEPSEASIDWSVTTAEGFDR S+ N S
Sbjct: 465 RRLAAANGNNNLMYCTRGSLDEAVTPSIAATECYEPSEASIDWSVTTAEGFDRGSVTNYS 524

Query: 513 -EAEESWTEKNNNNNNNNRRRSSSGNGLLSCRSEKAVS-VGPQPV-------MKHVSSR- 554
             A E        +     ++  +G  LLSCR EKAVS VGP PV       M+HVSSR 
Sbjct: 525 ATAPEVDDVAATRHGGGISKKKGNGGMLLSCRCEKAVSVVGPNPVKCVAGSGMRHVSSRT 558

BLAST of Cucsa.337160 vs. TAIR10
Match: AT5G04190.1 (AT5G04190.1 phytochrome kinase substrate 4)

HSP 1 Score: 120.9 bits (302), Expect = 2.4e-27
Identity = 131/403 (32.51%), Postives = 165/403 (40.94%), Query Frame = 1

Query: 166 TASSEASWNSQTGLLSNPPGAISVSVLRGDSDHHSGRKTRKPPSSSSLAARWIFRSSKCP 225
           TASSEASWNSQTGLLSN          R  SD    R ++K P       RW FR   CP
Sbjct: 100 TASSEASWNSQTGLLSNKN--------RQGSDRDGRRSSKKGP-------RWFFRRRACP 159

Query: 226 CTGKKSVQVQESKV-VLDPKT-SPPYINNTTVGGHSQSQSESPPSEKTSGENTMLLQHPD 285
           C+  KSVQVQESK  +  PKT S   ++N  V  H Q+ S   P   T   NT+      
Sbjct: 160 CSSSKSVQVQESKPRIAVPKTGSDRIVSNRIVHSH-QTISSPEPIRLTIPSNTVT---RS 219

Query: 286 KDVVWGSQRRFPPNLLLQGHTTQRVIASTGFTFPILKNNNNNNGDIPTRAIGH---VLIE 345
            D     + R P                + F+FP L   +  + +     + H   V IE
Sbjct: 220 IDYTANKEARAP---------------VSNFSFPTLNETSQLSENPKNPVLNHIKPVRIE 279

Query: 346 DPPRDSLEVFNPSSARDSGNGGCSSLKSRILASVAASGGNATIVNDIDDVASDASSDLFE 405
                   V NP+S +                          ++ D ++  SDASSDLFE
Sbjct: 280 PALLPIKPVLNPTSPKG-------------------------VIID-EEATSDASSDLFE 339

Query: 406 IESFSTQTASTTTASYPAMFHRRDSMELEARRLGLTSAATRRSLDEPMTPSTDWYEPSEA 465
           IESFSTQTA+   A  P +   RDSME      G                    YEPSEA
Sbjct: 340 IESFSTQTAARPWA--PPV---RDSMEETVSEYG--------------------YEPSEA 399

Query: 466 SIDWSVTTAE-------GFDRASIANMSEAEESWTEKNNNNNNNNRRRSSSGNGLLSCRS 525
           S+ WSV TAE        F R ++++ S A   + +K                GLL+C  
Sbjct: 400 SVTWSVMTAEPASAVAANFSRIALSSSSTAFSGYDKKR--------------TGLLNCHC 401

Query: 526 EKAVSVG-----PQPVMKHVSSRPPLGKKPPLARSNSAHLSLT 552
           EKAV V       QPV          GK   L  + S+ LS+T
Sbjct: 460 EKAVMVNGDKRLVQPVKSVGVQNDVAGK--VLCNNGSSKLSVT 401

BLAST of Cucsa.337160 vs. NCBI nr
Match: gi|449437954|ref|XP_004136755.1| (PREDICTED: protein PHYTOCHROME KINASE SUBSTRATE 4 [Cucumis sativus])

HSP 1 Score: 1115.9 bits (2885), Expect = 0.0e+00
Identity = 554/554 (100.00%), Postives = 554/554 (100.00%), Query Frame = 1

Query: 1   MSLINRGLSEKTVFGYNNNNDMKEKRETANTNSIPSYITHSDHQHHHFLARCTIVDDSSE 60
           MSLINRGLSEKTVFGYNNNNDMKEKRETANTNSIPSYITHSDHQHHHFLARCTIVDDSSE
Sbjct: 1   MSLINRGLSEKTVFGYNNNNDMKEKRETANTNSIPSYITHSDHQHHHFLARCTIVDDSSE 60

Query: 61  LSIFDAKKYFNEVSANNNINKVSPIMNIDSMSMSMSEQCMDHSVASSQDQDSEKDYDLSK 120
           LSIFDAKKYFNEVSANNNINKVSPIMNIDSMSMSMSEQCMDHSVASSQDQDSEKDYDLSK
Sbjct: 61  LSIFDAKKYFNEVSANNNINKVSPIMNIDSMSMSMSEQCMDHSVASSQDQDSEKDYDLSK 120

Query: 121 SKPWTCGVAHPSSGVVSKFSPTTASTIDGHHRRSYRARSFHSATPTASSEASWNSQTGLL 180
           SKPWTCGVAHPSSGVVSKFSPTTASTIDGHHRRSYRARSFHSATPTASSEASWNSQTGLL
Sbjct: 121 SKPWTCGVAHPSSGVVSKFSPTTASTIDGHHRRSYRARSFHSATPTASSEASWNSQTGLL 180

Query: 181 SNPPGAISVSVLRGDSDHHSGRKTRKPPSSSSLAARWIFRSSKCPCTGKKSVQVQESKVV 240
           SNPPGAISVSVLRGDSDHHSGRKTRKPPSSSSLAARWIFRSSKCPCTGKKSVQVQESKVV
Sbjct: 181 SNPPGAISVSVLRGDSDHHSGRKTRKPPSSSSLAARWIFRSSKCPCTGKKSVQVQESKVV 240

Query: 241 LDPKTSPPYINNTTVGGHSQSQSESPPSEKTSGENTMLLQHPDKDVVWGSQRRFPPNLLL 300
           LDPKTSPPYINNTTVGGHSQSQSESPPSEKTSGENTMLLQHPDKDVVWGSQRRFPPNLLL
Sbjct: 241 LDPKTSPPYINNTTVGGHSQSQSESPPSEKTSGENTMLLQHPDKDVVWGSQRRFPPNLLL 300

Query: 301 QGHTTQRVIASTGFTFPILKNNNNNNGDIPTRAIGHVLIEDPPRDSLEVFNPSSARDSGN 360
           QGHTTQRVIASTGFTFPILKNNNNNNGDIPTRAIGHVLIEDPPRDSLEVFNPSSARDSGN
Sbjct: 301 QGHTTQRVIASTGFTFPILKNNNNNNGDIPTRAIGHVLIEDPPRDSLEVFNPSSARDSGN 360

Query: 361 GGCSSLKSRILASVAASGGNATIVNDIDDVASDASSDLFEIESFSTQTASTTTASYPAMF 420
           GGCSSLKSRILASVAASGGNATIVNDIDDVASDASSDLFEIESFSTQTASTTTASYPAMF
Sbjct: 361 GGCSSLKSRILASVAASGGNATIVNDIDDVASDASSDLFEIESFSTQTASTTTASYPAMF 420

Query: 421 HRRDSMELEARRLGLTSAATRRSLDEPMTPSTDWYEPSEASIDWSVTTAEGFDRASIANM 480
           HRRDSMELEARRLGLTSAATRRSLDEPMTPSTDWYEPSEASIDWSVTTAEGFDRASIANM
Sbjct: 421 HRRDSMELEARRLGLTSAATRRSLDEPMTPSTDWYEPSEASIDWSVTTAEGFDRASIANM 480

Query: 481 SEAEESWTEKNNNNNNNNRRRSSSGNGLLSCRSEKAVSVGPQPVMKHVSSRPPLGKKPPL 540
           SEAEESWTEKNNNNNNNNRRRSSSGNGLLSCRSEKAVSVGPQPVMKHVSSRPPLGKKPPL
Sbjct: 481 SEAEESWTEKNNNNNNNNRRRSSSGNGLLSCRSEKAVSVGPQPVMKHVSSRPPLGKKPPL 540

Query: 541 ARSNSAHLSLTFAA 555
           ARSNSAHLSLTFAA
Sbjct: 541 ARSNSAHLSLTFAA 554

BLAST of Cucsa.337160 vs. NCBI nr
Match: gi|700204237|gb|KGN59370.1| (hypothetical protein Csa_3G814370 [Cucumis sativus])

HSP 1 Score: 1115.9 bits (2885), Expect = 0.0e+00
Identity = 554/554 (100.00%), Postives = 554/554 (100.00%), Query Frame = 1

Query: 1   MSLINRGLSEKTVFGYNNNNDMKEKRETANTNSIPSYITHSDHQHHHFLARCTIVDDSSE 60
           MSLINRGLSEKTVFGYNNNNDMKEKRETANTNSIPSYITHSDHQHHHFLARCTIVDDSSE
Sbjct: 9   MSLINRGLSEKTVFGYNNNNDMKEKRETANTNSIPSYITHSDHQHHHFLARCTIVDDSSE 68

Query: 61  LSIFDAKKYFNEVSANNNINKVSPIMNIDSMSMSMSEQCMDHSVASSQDQDSEKDYDLSK 120
           LSIFDAKKYFNEVSANNNINKVSPIMNIDSMSMSMSEQCMDHSVASSQDQDSEKDYDLSK
Sbjct: 69  LSIFDAKKYFNEVSANNNINKVSPIMNIDSMSMSMSEQCMDHSVASSQDQDSEKDYDLSK 128

Query: 121 SKPWTCGVAHPSSGVVSKFSPTTASTIDGHHRRSYRARSFHSATPTASSEASWNSQTGLL 180
           SKPWTCGVAHPSSGVVSKFSPTTASTIDGHHRRSYRARSFHSATPTASSEASWNSQTGLL
Sbjct: 129 SKPWTCGVAHPSSGVVSKFSPTTASTIDGHHRRSYRARSFHSATPTASSEASWNSQTGLL 188

Query: 181 SNPPGAISVSVLRGDSDHHSGRKTRKPPSSSSLAARWIFRSSKCPCTGKKSVQVQESKVV 240
           SNPPGAISVSVLRGDSDHHSGRKTRKPPSSSSLAARWIFRSSKCPCTGKKSVQVQESKVV
Sbjct: 189 SNPPGAISVSVLRGDSDHHSGRKTRKPPSSSSLAARWIFRSSKCPCTGKKSVQVQESKVV 248

Query: 241 LDPKTSPPYINNTTVGGHSQSQSESPPSEKTSGENTMLLQHPDKDVVWGSQRRFPPNLLL 300
           LDPKTSPPYINNTTVGGHSQSQSESPPSEKTSGENTMLLQHPDKDVVWGSQRRFPPNLLL
Sbjct: 249 LDPKTSPPYINNTTVGGHSQSQSESPPSEKTSGENTMLLQHPDKDVVWGSQRRFPPNLLL 308

Query: 301 QGHTTQRVIASTGFTFPILKNNNNNNGDIPTRAIGHVLIEDPPRDSLEVFNPSSARDSGN 360
           QGHTTQRVIASTGFTFPILKNNNNNNGDIPTRAIGHVLIEDPPRDSLEVFNPSSARDSGN
Sbjct: 309 QGHTTQRVIASTGFTFPILKNNNNNNGDIPTRAIGHVLIEDPPRDSLEVFNPSSARDSGN 368

Query: 361 GGCSSLKSRILASVAASGGNATIVNDIDDVASDASSDLFEIESFSTQTASTTTASYPAMF 420
           GGCSSLKSRILASVAASGGNATIVNDIDDVASDASSDLFEIESFSTQTASTTTASYPAMF
Sbjct: 369 GGCSSLKSRILASVAASGGNATIVNDIDDVASDASSDLFEIESFSTQTASTTTASYPAMF 428

Query: 421 HRRDSMELEARRLGLTSAATRRSLDEPMTPSTDWYEPSEASIDWSVTTAEGFDRASIANM 480
           HRRDSMELEARRLGLTSAATRRSLDEPMTPSTDWYEPSEASIDWSVTTAEGFDRASIANM
Sbjct: 429 HRRDSMELEARRLGLTSAATRRSLDEPMTPSTDWYEPSEASIDWSVTTAEGFDRASIANM 488

Query: 481 SEAEESWTEKNNNNNNNNRRRSSSGNGLLSCRSEKAVSVGPQPVMKHVSSRPPLGKKPPL 540
           SEAEESWTEKNNNNNNNNRRRSSSGNGLLSCRSEKAVSVGPQPVMKHVSSRPPLGKKPPL
Sbjct: 489 SEAEESWTEKNNNNNNNNRRRSSSGNGLLSCRSEKAVSVGPQPVMKHVSSRPPLGKKPPL 548

Query: 541 ARSNSAHLSLTFAA 555
           ARSNSAHLSLTFAA
Sbjct: 549 ARSNSAHLSLTFAA 562

BLAST of Cucsa.337160 vs. NCBI nr
Match: gi|659084953|ref|XP_008443162.1| (PREDICTED: protein PHYTOCHROME KINASE SUBSTRATE 4 [Cucumis melo])

HSP 1 Score: 1051.2 bits (2717), Expect = 6.3e-304
Identity = 533/555 (96.04%), Postives = 540/555 (97.30%), Query Frame = 1

Query: 1   MSLINRGLSEKTVFGYNNNNDMKEKRETANTNSIPSYITHSDHQHHHFLARCTIVDDSSE 60
           MS+INRGLSEKTVFGYNNNNDMKEKRETANTNSIPSYITHSDHQHHHFLARCTIVDDSSE
Sbjct: 11  MSVINRGLSEKTVFGYNNNNDMKEKRETANTNSIPSYITHSDHQHHHFLARCTIVDDSSE 70

Query: 61  LSIFDAKKYFNEVSANNNINKVSPIMNIDSMSMSMSEQCMDHSVASSQDQDSEKDYDLSK 120
           LSIFDAKKYFNEVSANNNINKVSPIMNI+SMSM+  EQCMD SVASSQDQDS+KDY+LSK
Sbjct: 71  LSIFDAKKYFNEVSANNNINKVSPIMNIESMSMT--EQCMDDSVASSQDQDSDKDYELSK 130

Query: 121 SKPWTCGVAHPSSGVVSKFSPTTASTIDGHHRRSYRARSFHSATPTASSEASWNSQTGLL 180
           SKPWTCGVAHPSSGVVSKFSPTTAS IDGHHRRSYRARSFHSATPTASSEASWNSQTGLL
Sbjct: 131 SKPWTCGVAHPSSGVVSKFSPTTAS-IDGHHRRSYRARSFHSATPTASSEASWNSQTGLL 190

Query: 181 SNPPGAISVSVLRGDSDHHSGRKTRKPPSSSSLAARWIFRSSKCPCTGKKSVQVQESKVV 240
           SNPPGAISVSVLRGDSDHHSGRKTRKPPSSSSLAARWIFRSSKCPCTGKKSVQVQESKVV
Sbjct: 191 SNPPGAISVSVLRGDSDHHSGRKTRKPPSSSSLAARWIFRSSKCPCTGKKSVQVQESKVV 250

Query: 241 LDPKTSPPYINNTTVGGHSQSQSESPPSEKTSGENTMLLQHPDKDVVWGSQRRFPPNLLL 300
           LDPKTSP YINNTTVGGHSQSQSESPPSEKTSGENTMLL HPDKDVVW SQRRFPPNLLL
Sbjct: 251 LDPKTSP-YINNTTVGGHSQSQSESPPSEKTSGENTMLLPHPDKDVVWSSQRRFPPNLLL 310

Query: 301 QGHTTQRVIASTGFTFPILKNNNNNNGDIPTRAIGHVLIEDPPRDSLEVFNPSSARDSGN 360
           QGHTTQRVIASTGFTFPILKNNN   GDIPTR IGHVLI+DPPRDSLEVFNPS+ARDSGN
Sbjct: 311 QGHTTQRVIASTGFTFPILKNNN---GDIPTRPIGHVLIQDPPRDSLEVFNPSTARDSGN 370

Query: 361 GGCSSLKSRILASVAASGGNATIVNDIDDVASDASSDLFEIESFSTQTASTTTASYPAMF 420
            GCSSLKSRILASVAASGGNATIVNDIDDVASDASSDLFEIESFSTQTASTTTASYPAMF
Sbjct: 371 SGCSSLKSRILASVAASGGNATIVNDIDDVASDASSDLFEIESFSTQTASTTTASYPAMF 430

Query: 421 HRRDSMELEARRLGLTSAATRRSLDEPMTPSTDWYEPSEASIDWSVTTAEGFDRASIANM 480
           HRRDSMELEARRLGL SAATRRSLDEPMTPSTDWYEPSEASIDWSVTTAEGFDRASIANM
Sbjct: 431 HRRDSMELEARRLGLASAATRRSLDEPMTPSTDWYEPSEASIDWSVTTAEGFDRASIANM 490

Query: 481 SEAEESWTEK-NNNNNNNNRRRSSSGNGLLSCRSEKAVSVGPQPVMKHVSSRPPLGKKPP 540
           SEAEESWTEK NNNNNNNNRRRSSSGNGLLSCRSEKAVSVGPQPV KHVSSRPPLGKKPP
Sbjct: 491 SEAEESWTEKNNNNNNNNNRRRSSSGNGLLSCRSEKAVSVGPQPVTKHVSSRPPLGKKPP 550

Query: 541 LARSNSAHLSLTFAA 555
           LARSNSAHLSLTFAA
Sbjct: 551 LARSNSAHLSLTFAA 558

BLAST of Cucsa.337160 vs. NCBI nr
Match: gi|802600242|ref|XP_012072841.1| (PREDICTED: protein PHYTOCHROME KINASE SUBSTRATE 4 [Jatropha curcas])

HSP 1 Score: 303.5 bits (776), Expect = 7.5e-79
Identity = 209/467 (44.75%), Postives = 264/467 (56.53%), Query Frame = 1

Query: 142 TTASTIDGHHRRSYRARSFHSATPTASSEASWNSQTGLLSNPPGAISVSVLRGDSDHHSG 201
           + +S++DG+ R +YR RSFH ATPTASSEASWNSQTGLLSNPPGAI+VS+    S H+  
Sbjct: 113 SASSSVDGYGR-NYRTRSFH-ATPTASSEASWNSQTGLLSNPPGAIAVSIRNPASGHNDE 172

Query: 202 RKTRKPPSSSSLAARWIFRSSKCPCTGKKSVQVQESKVVLDPKTSPPYINNTTVGGHSQS 261
           +K R        A++W+    +CPC+GKKSVQV+E   + +P+T P  +N+      +  
Sbjct: 173 KKKR-------FASKWLL-GRRCPCSGKKSVQVEEK--LSEPRT-PSRLNHKFKEDRNLK 232

Query: 262 QSESPPS-EKTSGENTMLLQHPD-----KDVVWGSQRRFPPNL----LLQGHTTQRVIAS 321
           +  +P S EK        LQ  +       ++     RFP  +    +L   T      +
Sbjct: 233 RQITPDSVEKCCAVTDHWLQRQEVIPISHTIISADNNRFPSGISHHRVLASSTRPFTDGT 292

Query: 322 TGFTFPILKNN-------------------NNNNGDIPTRAIGHVLIEDPPRDSLEVFNP 381
            GF+FPIL                      + NN   PT     + +EDPPRDSLEVF P
Sbjct: 293 NGFSFPILNQTTLSSLSPMKLALNGLPSPTSYNNPPPPTP----LPLEDPPRDSLEVFQP 352

Query: 382 SSARDSGNGGCSSLKSRILASVAASGGNATIVNDIDDVASDASSDLFEIESFSTQTASTT 441
           S    S   G + L+ R   +  AS  +   + D DD+ASDASSDLFEIESFSTQT S  
Sbjct: 353 SDDPVSTKTGAAELQRRQSFTFQASPKSRVAITD-DDIASDASSDLFEIESFSTQTTS-- 412

Query: 442 TASYPAMFHRRDSME----LEARRLG-LTSAATRRSLDEPMTPSTDWYEPSEASIDWSVT 501
              YP   HRRDSM+      ARR G   S   R+SLD  + P+  +YEPSEASIDWSVT
Sbjct: 413 ---YPMYNHRRDSMDEASIFNARRSGGSNSGYCRQSLDALIAPTECYYEPSEASIDWSVT 472

Query: 502 TAEGFDRASIANMSEAEESW------TEKNNNNNNNNRRRSSSGN-GLLSCRSEKAVSVG 554
           TAEGFD+AS+ N SEAEE+       +EK++ +    +RR   GN GLLSCR EKAVSVG
Sbjct: 473 TAEGFDKASVTNFSEAEETTIMRRHESEKSSGSGGCGKRRGGEGNGGLLSCRCEKAVSVG 532

BLAST of Cucsa.337160 vs. NCBI nr
Match: gi|802600242|ref|XP_012072841.1| (PREDICTED: protein PHYTOCHROME KINASE SUBSTRATE 4 [Jatropha curcas])

HSP 1 Score: 37.7 bits (86), Expect = 7.6e+01
Identity = 25/81 (30.86%), Postives = 43/81 (53.09%), Query Frame = 1

Query: 36  SYITHSDHQHHHFLARCTIVDDSSELSIFDAKKYFNEVSANNN---INKVSP--IMNIDS 95
           S+ +H  HQ     ++     + SELSIFDA+KYFNE S + +     +VSP  ++N++ 
Sbjct: 43  SFSSHLKHQQQQLNSQA----EDSELSIFDAQKYFNENSNSGDSRMSKRVSPVNVINLER 102

Query: 96  MSMSMSEQCMDHSVASSQDQD 112
           +S +     +    ++S   D
Sbjct: 103 ISQTCDFTSLPRFSSASSSVD 119


HSP 2 Score: 278.9 bits (712), Expect = 2.0e-71
Identity = 215/500 (43.00%), Postives = 270/500 (54.00%), Query Frame = 1

Query: 127 GVAHPSSGVVSKFSPTTASTIDGHHRRSYRARSFHSATPTASSEASWNSQTGLLSNPPGA 186
           G  H  S  V++ S  + S+++G+ R +YRARSFH ATPTASSEASWNSQTGLL NPPG 
Sbjct: 87  GAEHCESQFVTRTS--SVSSVEGYGR-NYRARSFH-ATPTASSEASWNSQTGLLCNPPGT 146

Query: 187 ISVSV--LRGDSDHHSGRKTRKPPSSSSLAARWIFRSSKCPCTGKKSVQVQESKVVLDPK 246
           I VSV  +  +  + SGRK             W F   KCPC+GKK+V++QE   V +P+
Sbjct: 147 IGVSVRSIGSEKKNVSGRK-------------WFF-GRKCPCSGKKAVEIQEK--VSEPR 206

Query: 247 TSPPYINNTTVGGHSQSQSESPPSEKTSG--------ENTMLLQHP--------DKDVVW 306
            SP  +N      H+       P+E++SG        ++   L  P        D+  + 
Sbjct: 207 -SPIRLN------HNLGSDPKRPTERSSGNTLQKVSKKSAKSLDSPHGHGRDWADRHDLI 266

Query: 307 GSQRRFPPNLLLQGHT-TQRVIAS-TGFTFPILKNNNNNNGDIPTRAIGHVLIEDPPRDS 366
            S  +FPP   L  +   +RV+AS TGF+FPIL         + T A   + +EDPPRDS
Sbjct: 267 PSPPKFPPATHLPSNQLARRVLASGTGFSFPILSQAPPVKL-VFTSARNPIPLEDPPRDS 326

Query: 367 LEVFNPSSARDSGNGGCSSLKSRILASVAASGGNATIVNDI----------DDVASDASS 426
           LE+F PS    S      S+  RI AS  A+    +               DDVASDASS
Sbjct: 327 LEIFRPSDDCISRKS-TDSIAPRIFASNPAAAERRSFTFSASPKSRGTAMDDDVASDASS 386

Query: 427 DLFEIESFSTQTASTTTAS--YPAMFHRRDSMELEARRLGLTSAATRRSLDEPMTPS--- 486
           DLFEIESF+TQT +TTT +  YP M+HRRDS++  +RR G      RRSLDEP TPS   
Sbjct: 387 DLFEIESFTTQTTTTTTTATNYP-MYHRRDSLDDASRRFGYC----RRSLDEPATPSVAP 446

Query: 487 TDWYEPSEASIDWSVTTAEGFDRASIANMSEAEESWTE-----------------KNNNN 546
           T+ YEPSEASIDWSVTTAEGFDR S+ N S +     +                  +N+ 
Sbjct: 447 TECYEPSEASIDWSVTTAEGFDRGSVTNFSMSASDIVDDVTLMRQELQRLGNGGFNDNDG 506

Query: 547 NNNNRRRSSSGNG-LLSCRSEKAVSVGPQP--------------------VMKHVSSRPP 554
               RRR   GNG L+SCR EKAVSVGP P                      +HVSSR  
Sbjct: 507 GGGGRRR---GNGMLMSCRCEKAVSVGPGPTPVKEGQWGRGGGPGYIGSSTSRHVSSRVG 549

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PKS4_ARATH4.3e-2632.51Protein PHYTOCHROME KINASE SUBSTRATE 4 OS=Arabidopsis thaliana GN=PKS4 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LBH5_CUCSA0.0e+00100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_3G814370 PE=4 SV=1[more]
A0A067KR89_JATCU5.2e-7944.75Uncharacterized protein OS=Jatropha curcas GN=JCGZ_05972 PE=4 SV=1[more]
A0A067KR89_JATCU5.3e+0130.86Uncharacterized protein OS=Jatropha curcas GN=JCGZ_05972 PE=4 SV=1[more]
F6HMU6_VITVI2.2e+0345.45Putative uncharacterized protein OS=Vitis vinifera GN=VIT_08s0056g00720 PE=4 SV=... [more]
B9S4J7_RICCO2.6e+0248.72Putative uncharacterized protein OS=Ricinus communis GN=RCOM_0809110 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G04190.12.4e-2732.51 phytochrome kinase substrate 4[more]
Match NameE-valueIdentityDescription
gi|449437954|ref|XP_004136755.1|0.0e+00100.00PREDICTED: protein PHYTOCHROME KINASE SUBSTRATE 4 [Cucumis sativus][more]
gi|700204237|gb|KGN59370.1|0.0e+00100.00hypothetical protein Csa_3G814370 [Cucumis sativus][more]
gi|659084953|ref|XP_008443162.1|6.3e-30496.04PREDICTED: protein PHYTOCHROME KINASE SUBSTRATE 4 [Cucumis melo][more]
gi|802600242|ref|XP_012072841.1|7.5e-7944.75PREDICTED: protein PHYTOCHROME KINASE SUBSTRATE 4 [Jatropha curcas][more]
gi|802600242|ref|XP_012072841.1|7.6e+0130.86PREDICTED: protein PHYTOCHROME KINASE SUBSTRATE 4 [Jatropha curcas][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.337160.1Cucsa.337160.1mRNA


Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR33781FAMILY NOT NAMEDcoord: 150..524
score: 1.6E-83coord: 56..110
score: 1.6
NoneNo IPR availablePANTHERPTHR33781:SF1PROTEIN PHYTOCHROME KINASE SUBSTRATE 4coord: 150..524
score: 1.6E-83coord: 56..110
score: 1.6