BLAST of Cucsa.337080 vs. Swiss-Prot
Match:
IDE1_ARATH (Insulin-degrading enzyme-like 1, peroxisomal OS=Arabidopsis thaliana GN=PXM16 PE=2 SV=1)
HSP 1 Score: 1379.8 bits (3570), Expect = 0.0e+00
Identity = 665/964 (68.98%), Postives = 803/964 (83.30%), Query Frame = 1
Query: 1 MAVGKEQMTH---EIVKARNDKREYKRIVLKNSLEVLLVSDPDTDKCAASMTVDVGSFSD 60
MAV K T EI+K R D REY+ IVLKN L+VLL+SDPDTDKCAASM+V VGSFSD
Sbjct: 1 MAVEKSNTTVGGVEILKPRTDNREYRMIVLKNLLQVLLISDPDTDKCAASMSVSVGSFSD 60
Query: 61 PEGLEGLAHFLEHMLFYASEKYPQEDSYSKYITEHGGSTNAFTASEETNYYFDVNADCFE 120
P+GLEGLAHFLEHMLFYASEKYP+EDSYSKYITEHGGSTNA+TASEETNY+FDVNADCF+
Sbjct: 61 PQGLEGLAHFLEHMLFYASEKYPEEDSYSKYITEHGGSTNAYTASEETNYHFDVNADCFD 120
Query: 121 EALDRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDVWRMHQLQCHISSESHPFHKFS 180
EALDRFAQFFIKPLMS DATMREIKAVDSENQKNLLSD WR+ QLQ H+S E HP+HKFS
Sbjct: 121 EALDRFAQFFIKPLMSADATMREIKAVDSENQKNLLSDGWRIRQLQKHLSKEDHPYHKFS 180
Query: 181 TGNWDTLEVQPKAKGLDTRHELLKFYENNYSSNVMHLVVYAKENLDKVQILVENIFQDIP 240
TGN DTL V+P+AKG+DTR EL+KFYE +YS+N+MHLVVY KE+LDK+Q LVE +FQ+I
Sbjct: 181 TGNMDTLHVRPQAKGVDTRSELIKFYEEHYSANIMHLVVYGKESLDKIQDLVERMFQEIQ 240
Query: 241 NHNCNRANFPGQPCTSEHLQVLVRAIPIKEGHKLRIIWPITPEIHHHKEGPCRYLGHLIG 300
N N FPGQPCT++HLQ+LV+AIPIK+GHKL + WP+TP IHH+ E P +YLGHLIG
Sbjct: 241 NTNKVVPRFPGQPCTADHLQILVKAIPIKQGHKLGVSWPVTPSIHHYDEAPSQYLGHLIG 300
Query: 301 HEGEGSLYYVLKTLGWATWLSADESSFTMNLSFFEVVIDLTDVGQEHMQDVIGLLFKYIS 360
HEGEGSL++ LKTLGWAT LSA E +T++ SFF+V IDLTD G EHMQ+++GLLF YI
Sbjct: 301 HEGEGSLFHALKTLGWATGLSAGEGEWTLDYSFFKVSIDLTDAGHEHMQEILGLLFNYIQ 360
Query: 361 LLKQSGIWQWIFDELSAICETKFHYADKIDPIDYVVNLSSSMQLYPPEDWLVGSSLPSKF 420
LL+Q+G+ QWIFDELSAICETKFHY DKI P+ Y+V+++S+MQ+YP +DWLVGSSLP+KF
Sbjct: 361 LLQQTGVCQWIFDELSAICETKFHYQDKIPPMSYIVDIASNMQIYPTKDWLVGSSLPTKF 420
Query: 421 NPKLIGTVLDQLSVDNVRIFWESKKFEGKTDKVEKWYGTAYSIEKVSGPLVQDWMQSAPD 480
NP ++ V+D+LS N RIFWES+KFEG+TDK E WY TAYS+EK++ +Q+W+QSAPD
Sbjct: 421 NPAIVQKVVDELSPSNFRIFWESQKFEGQTDKAEPWYNTAYSLEKITSSTIQEWVQSAPD 480
Query: 481 VKLHLPASNIFIPTDLSPKCACEKVKFPVLLKKSSYSSIWYKPDTMFSTPKAYVKIDFIC 540
V LHLPA N+FIPTDLS K A +K PVLL+K+ +S +WYKPDTMFS PKAYVK+DF C
Sbjct: 481 VHLHLPAPNVFIPTDLSLKDADDKETVPVLLRKTPFSRLWYKPDTMFSKPKAYVKMDFNC 540
Query: 541 PHADISPEAEVLTAIFTRLLVDYLNEYAYYAEVAGLYYGINLVESGFQVSLNGYNHKLRI 600
P A SP+A VLT IFTRLL+DYLNEYAYYA+VAGLYYG++L ++GF+++L GYNHKLRI
Sbjct: 541 PLAVSSPDAAVLTDIFTRLLMDYLNEYAYYAQVAGLYYGVSLSDNGFELTLLGYNHKLRI 600
Query: 601 LLETIVTKIANFSVKPDRFLVIKETLLKNYQNFKFQQPYQQAFYYCSLILGDRTWPLMDK 660
LLET+V KIANF VKPDRF VIKET+ K YQN+KF+QPY QA YYCSLIL D+TWP ++
Sbjct: 601 LLETVVGKIANFEVKPDRFAVIKETVTKEYQNYKFRQPYHQAMYYCSLILQDQTWPWTEE 660
Query: 661 LAILSNLGAQDLDKFVPTLLSSAYLECFIAGNIERTEAESMIDHIEDKFFKGSNPISRPL 720
L +LS+L A+D+ KFVP LLS ++EC+IAGN+E EAESM+ HIED F PI RPL
Sbjct: 661 LDVLSHLEAEDVAKFVPMLLSRTFIECYIAGNVENNEAESMVKHIEDVLFNDPKPICRPL 720
Query: 721 HPSQYPPNRIVKLERSIGYFYSAEGLNSNNENSALVHYIQVHRDEFLQNVKLQLFAHVAQ 780
PSQ+ NR+VKL + YFY +G N ++ENSALVHYIQVHRD+F N+KLQLF VA+
Sbjct: 721 FPSQHLTNRVVKLGEGMKYFYHQDGSNPSDENSALVHYIQVHRDDFSMNIKLQLFGLVAK 780
Query: 781 QAAFHQLRSVEQLGYITALAQRNDCGIRGVQFIIQSTVKGPRDIDLRVEAFLEMFEKKLV 840
QA FHQLR+VEQLGYITALAQRND GI GVQFIIQS+VKGP ID RVE+ L+ FE KL
Sbjct: 781 QATFHQLRTVEQLGYITALAQRNDSGIYGVQFIIQSSVKGPGHIDSRVESLLKNFESKLY 840
Query: 841 EMTVDEFQSNVNALVDAKLEKFKNLREEARFYWGEISEGTLKFDRRESEVAILKTLTHQD 900
EM+ ++F+SNV AL+D KLEK KNL+EE+RFYW EI GTLKF+R+E+EV+ LK L Q+
Sbjct: 841 EMSNEDFKSNVTALIDMKLEKHKNLKEESRFYWREIQSGTLKFNRKEAEVSALKQLQKQE 900
Query: 901 LINFFNEHIKVGAPRKKSLSVRVYGNLHSKEYSGDLNQPVQPNTVKIDDIFSFRRSQPLY 960
LI+FF+E+IKVGA RKKSLS+RVYG+ H KE + D ++ P +V+I+DI FR+SQPL+
Sbjct: 901 LIDFFDEYIKVGAARKKSLSIRVYGSQHLKEMASDKDEVPSP-SVEIEDIVGFRKSQPLH 960
Query: 961 GSFK 962
GSF+
Sbjct: 961 GSFR 963
BLAST of Cucsa.337080 vs. Swiss-Prot
Match:
IDE2_ARATH (Insulin-degrading enzyme-like 2 OS=Arabidopsis thaliana GN=At3g57470 PE=2 SV=2)
HSP 1 Score: 1283.1 bits (3319), Expect = 0.0e+00
Identity = 630/966 (65.22%), Postives = 764/966 (79.09%), Query Frame = 1
Query: 1 MAVGKEQMTH-----EIVKARNDKREYKRIVLKNSLEVLLVSDPDTDKCAASMTVDVGSF 60
MAVG E T EI+K R DKREY+RIVLKNSLEVLL+SDP+TDKCAASM V VGSF
Sbjct: 1 MAVGMENATASGECGEILKPRTDKREYRRIVLKNSLEVLLISDPETDKCAASMNVSVGSF 60
Query: 61 SDPEGLEGLAHFLEHMLFYASEKYPQEDSYSKYITEHGGSTNAFTASEETNYYFDVNADC 120
+DPEGLEGLAHFLEHMLFYASEKYP+EDSYSKYITEHGGSTNA+T+SE+TNY+FD+N D
Sbjct: 61 TDPEGLEGLAHFLEHMLFYASEKYPEEDSYSKYITEHGGSTNAYTSSEDTNYHFDINTDS 120
Query: 121 FEEALDRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDVWRMHQLQCHISSESHPFHK 180
F EALDRFAQFFI+PLMS DATMREIKAVDSE+Q NLLSD WRM QLQ H+S E HP+HK
Sbjct: 121 FYEALDRFAQFFIQPLMSTDATMREIKAVDSEHQNNLLSDSWRMAQLQKHLSREDHPYHK 180
Query: 181 FSTGNWDTLEVQPKAKGLDTRHELLKFYENNYSSNVMHLVVYAKENLDKVQILVENIFQD 240
FSTGN DTL V+P+ G+DTR EL+KFY+ +YS+N+MHLVVY KENLDK Q LVE +FQ
Sbjct: 181 FSTGNMDTLHVRPEENGVDTRSELIKFYDEHYSANIMHLVVYGKENLDKTQGLVEALFQG 240
Query: 241 IPNHNCNRANFPGQPCTSEHLQVLVRAIPIKEGHKLRIIWPITPEIHHHKEGPCRYLGHL 300
I N N FPGQPCT +HLQVLV+A+PI +GH+L + WP+TP I H++E PCRYLG L
Sbjct: 241 IRNTNQGIPRFPGQPCTLDHLQVLVKAVPIMQGHELSVSWPVTPSISHYEEAPCRYLGDL 300
Query: 301 IGHEGEGSLYYVLKTLGWATWLSADESSFTMNLSFFEVVIDLTDVGQEHMQDVIGLLFKY 360
IGHEGEGSL++ LK LGWAT L A E+ ++M SFF V IDLTD G EHMQD++GLLF+Y
Sbjct: 301 IGHEGEGSLFHALKILGWATGLYAGEADWSMEYSFFNVSIDLTDAGHEHMQDILGLLFEY 360
Query: 361 ISLLKQSGIWQWIFDELSAICETKFHYADKIDPIDYVVNLSSSMQLYPPEDWLVGSSLPS 420
I +L+QSG+ QWIFDELSAICE +FHY KIDPI Y V++SS+M++YP + WLVGSSLPS
Sbjct: 361 IKVLQQSGVSQWIFDELSAICEAEFHYQAKIDPISYAVDISSNMKIYPTKHWLVGSSLPS 420
Query: 421 KFNPKLIGTVLDQLSVDNVRIFWESKKFEGKTDKVEKWYGTAYSIEKVSGPLVQDWMQSA 480
KFNP ++ VLD+LS +NVRIFWES KFEG+TDKVE WY TAYS+EK++ +Q+WMQSA
Sbjct: 421 KFNPAIVQKVLDELSPNNVRIFWESNKFEGQTDKVEPWYNTAYSLEKITKFTIQEWMQSA 480
Query: 481 PDVKLHLPASNIFIPTDLSPKCACEKVKFPVLLKKSSYSSIWYKPDTMFSTPKAYVKIDF 540
PDV L LP N+FIPTD S K +K FPVLL+K+SYS +WYKPDT F PKAYVK+DF
Sbjct: 481 PDVNLLLPTPNVFIPTDFSLKDLKDKDIFPVLLRKTSYSRLWYKPDTKFFKPKAYVKMDF 540
Query: 541 ICPHADISPEAEVLTAIFTRLLVDYLNEYAYYAEVAGLYYGINLVESGFQVSLNGYNHKL 600
CP A SP+A VL+ IF LLVDYLNEYAYYA+ AGL YG++L ++GF++SL G+NHKL
Sbjct: 541 NCPLAVSSPDAAVLSDIFVWLLVDYLNEYAYYAQAAGLDYGLSLSDNGFELSLAGFNHKL 600
Query: 601 RILLETIVTKIANFSVKPDRFLVIKETLLKNYQNFKFQQPYQQAFYYCSLILGDRTWPLM 660
RILLE ++ KIA F VKPDRF VIKET+ K YQN KFQQP++QA YCSL+L D+ WP
Sbjct: 601 RILLEAVIQKIAKFEVKPDRFSVIKETVTKAYQNNKFQQPHEQATNYCSLVLQDQIWPWT 660
Query: 661 DKLAILSNLGAQDLDKFVPTLLSSAYLECFIAGNIERTEAESMIDHIEDKFFKGSNPISR 720
++L LS+L A+DL FVP LLS ++EC+IAGN+E+ EAESM+ HIED F S PI R
Sbjct: 661 EELDALSHLEAEDLANFVPMLLSRTFVECYIAGNVEKDEAESMVKHIEDVLFTDSKPICR 720
Query: 721 PLHPSQYPPNRIVKLERSIGYFYSAEGLNSNNENSALVHYIQVHRDEFLQNVKLQLFAHV 780
PL PSQ+ NR+ +L + +FY EG NS++ENSALVHYIQVH+DEF N KLQLF +
Sbjct: 721 PLFPSQFLTNRVTELGTGMKHFYYQEGSNSSDENSALVHYIQVHKDEFSMNSKLQLFELI 780
Query: 781 AQQAAFHQLRSVEQLGYITALAQRNDCGIRGVQFIIQSTVKGPRDIDLRVEAFLEMFEKK 840
A+Q FHQLR++EQLGYIT+L+ ND G+ GVQFIIQS+VKGP ID RVE+ L+ E K
Sbjct: 781 AKQDTFHQLRTIEQLGYITSLSLSNDSGVYGVQFIIQSSVKGPGHIDSRVESLLKDLESK 840
Query: 841 LVEMTVDEFQSNVNALVDAKLEKFKNLREEARFYWGEISEGTLKFDRRESEVAILKTLTH 900
M+ +EF+SNV L+D KLEK KNL EE+ FYW EI GTLKF+R ++EVA L+ L
Sbjct: 841 FYNMSDEEFKSNVTNLIDMKLEKDKNLDEESWFYWAEIQTGTLKFNRIDAEVAALRLLKK 900
Query: 901 QDLINFFNEHIKVGAPRKKSLSVRVYGNLHSKEYSGDLNQPVQPNTVKIDDIFSFRRSQP 960
+ I+FF+E+IKV AP KKSLS+ VYGN H KE D ++ + +++I+DI FR+SQP
Sbjct: 901 DEWIDFFDEYIKVDAPNKKSLSICVYGNQHLKEMRNDKDK-IPSTSIEIEDIVCFRKSQP 960
Query: 961 LYGSFK 962
LYGS K
Sbjct: 961 LYGSLK 965
BLAST of Cucsa.337080 vs. Swiss-Prot
Match:
IDE_BOVIN (Insulin-degrading enzyme OS=Bos taurus GN=IDE PE=2 SV=1)
HSP 1 Score: 697.6 bits (1799), Expect = 1.9e-199
Identity = 378/963 (39.25%), Postives = 548/963 (56.91%), Query Frame = 1
Query: 6 EQMTHEIVKARNDKREYKRIVLKNSLEVLLVSDPDTDKCAASMTVDVGSFSDPEGLEGLA 65
+++ H I+K+ DKREY+ + L N ++VLLVSDP TDK +A++ V +GS SDP + GL+
Sbjct: 48 KRIGHHIIKSHEDKREYRGLELANGIKVLLVSDPTTDKSSAALDVHIGSLSDPPNIAGLS 107
Query: 66 HFLEHMLFYASEKYPQEDSYSKYITEHGGSTNAFTASEETNYYFDVNADCFEEALDRFAQ 125
HF EHMLF ++KYP+E+ YS++++EH GS+NAFT+ E TNYYFDV+ + E ALDRFAQ
Sbjct: 108 HFCEHMLFLGTKKYPKENEYSQFLSEHAGSSNAFTSGEHTNYYFDVSHEHLEGALDRFAQ 167
Query: 126 FFIKPLMSPDATMREIKAVDSENQKNLLSDVWRMHQLQCHISSESHPFHKFSTGNWDTLE 185
FF+ PL RE+ AVDSE++KN+++D WR+ QL+ + HPF KF TGN TLE
Sbjct: 168 FFLCPLFDESCKDREVNAVDSEHEKNVMNDAWRLFQLEKATGNPKHPFSKFGTGNKYTLE 227
Query: 186 VQPKAKGLDTRHELLKFYENNYSSNVMHLVVYAKENLDKVQILVENIFQDIPNHNCNRAN 245
+P +G+D R ELLKF+ YSSN+M + V +E+LD + LV +F ++ N N
Sbjct: 228 TRPNQEGIDVRQELLKFHSTYYSSNLMAICVLGRESLDDLTNLVVKLFSEVENKNVPLPE 287
Query: 246 FPGQPCTSEHLQVLVRAIPIKEGHKLRIIWPITPEIHHHKEGPCRYLGHLIGHEGEGSLY 305
FP P EHL+ L + +PIK+ L + +PI ++K P YLGHLIGHEG GSL
Sbjct: 288 FPEHPFQEEHLKQLYKIVPIKDIRNLYVTFPIPDLQKYYKSNPGHYLGHLIGHEGPGSLL 347
Query: 306 YVLKTLGWATWLSADESSFTMNLSFFEVVIDLTDVGQEHMQDVIGLLFKYISLLKQSGIW 365
LK+ GW L + FF + +DLT+ G H++D+I +F+YI L+ G
Sbjct: 348 SELKSKGWVNTLVGGQKEGARGFMFFIINVDLTEEGLLHVEDIILHMFQYIQKLRAEGPQ 407
Query: 366 QWIFDELSAICETKFHYADKIDPIDYVVNLSSSMQLYPPEDWLVGSSLPSKFNPKLIGTV 425
+W+F E + F + DK P Y ++ + YP E+ L L +F P LI V
Sbjct: 408 EWVFQECKDLNAVAFRFKDKERPRGYTSKIAGILHYYPLEEVLTAEYLLEEFRPDLIEMV 467
Query: 426 LDQLSVDNVRIFWESKKFEGKTDKVEKWYGTAYSIEKVSGPLVQDWMQSAPDVKLHLPAS 485
LD+L +NVR+ SK FEGKTD+ E+WYGT Y E + +++ W + + K LP
Sbjct: 468 LDKLRPENVRVAIVSKSFEGKTDRTEEWYGTQYKQEAIPDEVIKKWQNADLNGKFKLPMK 527
Query: 486 NIFIPTDLS-PKCACEKVKFPVLLKKSSYSSIWYKPDTMFSTPKAYVKIDFICPHADISP 545
N FIPT+ E +P L+K ++ S +W+K D F PKA + +F P A + P
Sbjct: 528 NEFIPTNFEILSLEKEATPYPSLIKDTAMSKLWFKQDDKFFLPKACLNFEFFSPFAYVDP 587
Query: 546 EAEVLTAIFTRLLVDYLNEYAYYAEVAGLYYGINLVESGFQVSLNGYNHKLRILLETIVT 605
+ ++ LL D LNEYAY AE+AGL Y + G +S+ GYN K ILL+ I+
Sbjct: 588 LHCNMAYLYLELLKDSLNEYAYAAELAGLSYDLQNTIYGMYLSVKGYNDKQPILLKKIIE 647
Query: 606 KIANFSVKPDRFLVIKETLLKNYQNFKFQQPYQQAFYYCSLILGDRTWPLMDKLAILSNL 665
K+A F + RF +IKE +++ NF+ +QP+Q A YY L++ + W + L ++
Sbjct: 648 KMATFEIDEKRFEIIKEAYMRSLNNFRAEQPHQHAMYYLRLLMTEVAWTKDELKEALDDV 707
Query: 666 GAQDLDKFVPTLLSSAYLECFIAGNIERTEAESMIDHIEDKFFKGSNPISRPLHPSQYPP 725
L F+P LLS ++E + GNI + A ++ +ED + ++ ++PL PSQ
Sbjct: 708 TLPRLKAFIPQLLSRLHIEALLHGNITKQAALGIMQMVEDTLIEHAH--TKPLLPSQLVR 767
Query: 726 NRIVKLERSIGYFYSAEGLNSNNENSALVHYIQVHRDEFLQNVKLQLFAHVAQQAAFHQL 785
R V+L + Y N + N + Y Q +N+ L+LF + + F+ L
Sbjct: 768 YREVQLPDRGWFVYQQR--NEVHNNCGIEIYYQTDMQSTSENMFLELFCQIISEPCFNTL 827
Query: 786 RSVEQLGYITALAQRNDCGIRGVQFIIQSTVKGPRDIDLRVEAFLEMFEKKLVEMTVDEF 845
R+ EQLGYI R GI+G++FIIQS K P ++ RVEAFL EK + +MT + F
Sbjct: 828 RTKEQLGYIVFSGPRRANGIQGLRFIIQSE-KPPHYLESRVEAFLITMEKSIEDMTEEAF 887
Query: 846 QSNVNALVDAKLEKFKNLREEARFYWGEISEGTLKFDRRESEVAILKTLTHQDLINFFNE 905
Q ++ AL +L+K K L E YWGEI FDR EVA LKTLT +D+I F+ E
Sbjct: 888 QKHIQALAIRRLDKPKKLSAECAKYWGEIISQQYNFDRDNIEVAYLKTLTKEDIIKFYKE 947
Query: 906 HIKVGAPRKKSLSVRVYGN-------LHSKEYSGDLN---QPVQPNTVKIDDIFSFRRSQ 958
+ V APR+ +SV V + D+N P P I ++ F+R
Sbjct: 948 MLAVDAPRRHKVSVHVLAREMDSCPVVGEFPCQNDINLSQAPALPQPEVIQNMTEFKRGL 1005
BLAST of Cucsa.337080 vs. Swiss-Prot
Match:
IDE_HUMAN (Insulin-degrading enzyme OS=Homo sapiens GN=IDE PE=1 SV=4)
HSP 1 Score: 693.3 bits (1788), Expect = 3.7e-198
Identity = 376/963 (39.04%), Postives = 548/963 (56.91%), Query Frame = 1
Query: 6 EQMTHEIVKARNDKREYKRIVLKNSLEVLLVSDPDTDKCAASMTVDVGSFSDPEGLEGLA 65
+++ + I K+ DKREY+ + L N ++VLL+SDP TDK +A++ V +GS SDP + GL+
Sbjct: 48 KRIGNHITKSPEDKREYRGLELANGIKVLLISDPTTDKSSAALDVHIGSLSDPPNIAGLS 107
Query: 66 HFLEHMLFYASEKYPQEDSYSKYITEHGGSTNAFTASEETNYYFDVNADCFEEALDRFAQ 125
HF EHMLF ++KYP+E+ YS++++EH GS+NAFT+ E TNYYFDV+ + E ALDRFAQ
Sbjct: 108 HFCEHMLFLGTKKYPKENEYSQFLSEHAGSSNAFTSGEHTNYYFDVSHEHLEGALDRFAQ 167
Query: 126 FFIKPLMSPDATMREIKAVDSENQKNLLSDVWRMHQLQCHISSESHPFHKFSTGNWDTLE 185
FF+ PL RE+ AVDSE++KN+++D WR+ QL+ + HPF KF TGN TLE
Sbjct: 168 FFLCPLFDESCKDREVNAVDSEHEKNVMNDAWRLFQLEKATGNPKHPFSKFGTGNKYTLE 227
Query: 186 VQPKAKGLDTRHELLKFYENNYSSNVMHLVVYAKENLDKVQILVENIFQDIPNHNCNRAN 245
+P +G+D R ELLKF+ YSSN+M + V +E+LD + LV +F ++ N N
Sbjct: 228 TRPNQEGIDVRQELLKFHSAYYSSNLMAVCVLGRESLDDLTNLVVKLFSEVENKNVPLPE 287
Query: 246 FPGQPCTSEHLQVLVRAIPIKEGHKLRIIWPITPEIHHHKEGPCRYLGHLIGHEGEGSLY 305
FP P EHL+ L + +PIK+ L + +PI ++K P YLGHLIGHEG GSL
Sbjct: 288 FPEHPFQEEHLKQLYKIVPIKDIRNLYVTFPIPDLQKYYKSNPGHYLGHLIGHEGPGSLL 347
Query: 306 YVLKTLGWATWLSADESSFTMNLSFFEVVIDLTDVGQEHMQDVIGLLFKYISLLKQSGIW 365
LK+ GW L + FF + +DLT+ G H++D+I +F+YI L+ G
Sbjct: 348 SELKSKGWVNTLVGGQKEGARGFMFFIINVDLTEEGLLHVEDIILHMFQYIQKLRAEGPQ 407
Query: 366 QWIFDELSAICETKFHYADKIDPIDYVVNLSSSMQLYPPEDWLVGSSLPSKFNPKLIGTV 425
+W+F E + F + DK P Y ++ + YP E+ L L +F P LI V
Sbjct: 408 EWVFQECKDLNAVAFRFKDKERPRGYTSKIAGILHYYPLEEVLTAEYLLEEFRPDLIEMV 467
Query: 426 LDQLSVDNVRIFWESKKFEGKTDKVEKWYGTAYSIEKVSGPLVQDWMQSAPDVKLHLPAS 485
LD+L +NVR+ SK FEGKTD+ E+WYGT Y E + +++ W + + K LP
Sbjct: 468 LDKLRPENVRVAIVSKSFEGKTDRTEEWYGTQYKQEAIPDEVIKKWQNADLNGKFKLPTK 527
Query: 486 NIFIPTDLS-PKCACEKVKFPVLLKKSSYSSIWYKPDTMFSTPKAYVKIDFICPHADISP 545
N FIPT+ E +P L+K ++ S +W+K D F PKA + +F P A + P
Sbjct: 528 NEFIPTNFEILPLEKEATPYPALIKDTAMSKLWFKQDDKFFLPKACLNFEFFSPFAYVDP 587
Query: 546 EAEVLTAIFTRLLVDYLNEYAYYAEVAGLYYGINLVESGFQVSLNGYNHKLRILLETIVT 605
+ ++ LL D LNEYAY AE+AGL Y + G +S+ GYN K ILL+ I+
Sbjct: 588 LHCNMAYLYLELLKDSLNEYAYAAELAGLSYDLQNTIYGMYLSVKGYNDKQPILLKKIIE 647
Query: 606 KIANFSVKPDRFLVIKETLLKNYQNFKFQQPYQQAFYYCSLILGDRTWPLMDKLAILSNL 665
K+A F + RF +IKE +++ NF+ +QP+Q A YY L++ + W + L ++
Sbjct: 648 KMATFEIDEKRFEIIKEAYMRSLNNFRAEQPHQHAMYYLRLLMTEVAWTKDELKEALDDV 707
Query: 666 GAQDLDKFVPTLLSSAYLECFIAGNIERTEAESMIDHIEDKFFKGSNPISRPLHPSQYPP 725
L F+P LLS ++E + GNI + A ++ +ED + ++ ++PL PSQ
Sbjct: 708 TLPRLKAFIPQLLSRLHIEALLHGNITKQAALGIMQMVEDTLIEHAH--TKPLLPSQLVR 767
Query: 726 NRIVKLERSIGYFYSAEGLNSNNENSALVHYIQVHRDEFLQNVKLQLFAHVAQQAAFHQL 785
R V+L + Y N + N + Y Q +N+ L+LF + + F+ L
Sbjct: 768 YREVQLPDRGWFVYQQR--NEVHNNCGIEIYYQTDMQSTSENMFLELFCQIISEPCFNTL 827
Query: 786 RSVEQLGYITALAQRNDCGIRGVQFIIQSTVKGPRDIDLRVEAFLEMFEKKLVEMTVDEF 845
R+ EQLGYI R GI+G++FIIQS K P ++ RVEAFL EK + +MT + F
Sbjct: 828 RTKEQLGYIVFSGPRRANGIQGLRFIIQSE-KPPHYLESRVEAFLITMEKSIEDMTEEAF 887
Query: 846 QSNVNALVDAKLEKFKNLREEARFYWGEISEGTLKFDRRESEVAILKTLTHQDLINFFNE 905
Q ++ AL +L+K K L E YWGEI FDR +EVA LKTLT +D+I F+ E
Sbjct: 888 QKHIQALAIRRLDKPKKLSAECAKYWGEIISQQYNFDRDNTEVAYLKTLTKEDIIKFYKE 947
Query: 906 HIKVGAPRKKSLSVRVYGN-------LHSKEYSGDLN---QPVQPNTVKIDDIFSFRRSQ 958
+ V APR+ +SV V + D+N P P I ++ F+R
Sbjct: 948 MLAVDAPRRHKVSVHVLAREMDSCPVVGEFPCQNDINLSQAPALPQPEVIQNMTEFKRGL 1005
BLAST of Cucsa.337080 vs. Swiss-Prot
Match:
IDE_RAT (Insulin-degrading enzyme OS=Rattus norvegicus GN=Ide PE=1 SV=1)
HSP 1 Score: 689.5 bits (1778), Expect = 5.3e-197
Identity = 377/964 (39.11%), Postives = 544/964 (56.43%), Query Frame = 1
Query: 6 EQMTHEIVKARNDKREYKRIVLKNSLEVLLVSDPDTDKCAASMTVDVGSFSDPEGLEGLA 65
+++ IVK+ DKREY+ + L N ++VLL+SDP TDK +A++ V +GS SDP + GL+
Sbjct: 48 QRIEDHIVKSPEDKREYRGLELANGIKVLLISDPTTDKSSAALDVHIGSLSDPPNIPGLS 107
Query: 66 HFLEHMLFYASEKYPQEDSYSKYITEHGGSTNAFTASEETNYYFDVNADCFEEALDRFAQ 125
HF EHMLF ++KYP+E+ YS++++EH GS+NAFT+ E TNYYFDV+ + E ALDRFAQ
Sbjct: 108 HFCEHMLFLGTKKYPKENEYSQFLSEHAGSSNAFTSGEHTNYYFDVSHEHLEGALDRFAQ 167
Query: 126 FFIKPLMSPDATMREIKAVDSENQKNLLSDVWRMHQLQCHISSESHPFHKFSTGNWDTLE 185
FF+ PL RE+ AVDSE++KN+++D WR+ QL+ + HPF KF TGN TLE
Sbjct: 168 FFLCPLFDASCKDREVNAVDSEHEKNVMNDAWRLFQLEKATGNPKHPFSKFGTGNKYTLE 227
Query: 186 VQPKAKGLDTRHELLKFYENNYSSNVMHLVVYAKENLDKVQILVENIFQDIPNHNCNRAN 245
+P +G+D R ELLKF+ YSSN+M + V +E+LD + LV +F ++ N N
Sbjct: 228 TRPNQEGIDVREELLKFHSTYYSSNLMAICVLGRESLDDLTNLVVKLFSEVENKNVPLPE 287
Query: 246 FPGQPCTSEHLQVLVRAIPIKEGHKLRIIWPITPEIHHHKEGPCRYLGHLIGHEGEGSLY 305
FP P EHL+ L + +PIK+ L + +PI ++K P YLGHLIGHEG GSL
Sbjct: 288 FPEHPFQEEHLKQLYKIVPIKDIRNLYVTFPIPDLQQYYKSNPGHYLGHLIGHEGPGSLL 347
Query: 306 YVLKTLGWATWLSADESSFTMNLSFFEVVIDLTDVGQEHMQDVIGLLFKYISLLKQSGIW 365
LK+ GW L + FF + +DLT+ G H++D+I +F+YI L+ G
Sbjct: 348 SELKSKGWVNTLVGGQKEGARGFMFFIINVDLTEEGLLHVEDIILHMFQYIQKLRAEGPQ 407
Query: 366 QWIFDELSAICETKFHYADKIDPIDYVVNLSSSMQLYPPEDWLVGSSLPSKFNPKLIGTV 425
+W+F E + F + DK P Y ++ + YP L L +F P LI V
Sbjct: 408 EWVFQECKDLNAVAFRFKDKERPRGYTSKIAGKLHYYPLNGVLTAEYLLEEFRPDLIDMV 467
Query: 426 LDQLSVDNVRIFWESKKFEGKTDKVEKWYGTAYSIEKVSGPLVQDWMQSAPDVKLHLPAS 485
LD+L +NVR+ SK FEGKTD+ E+WYGT Y E + ++Q W + + K LP
Sbjct: 468 LDKLRPENVRVAIVSKSFEGKTDRTEQWYGTQYKQEAIPEDVIQKWQNADLNGKFKLPTK 527
Query: 486 NIFIPTDLSPKCACEK--VKFPVLLKKSSYSSIWYKPDTMFSTPKAYVKIDFICPHADIS 545
N FIPT+ A EK +P L+K ++ S +W+K D F PKA + +F P A +
Sbjct: 528 NEFIPTNFE-ILALEKDATPYPALIKDTAMSKLWFKQDDKFFLPKACLNFEFFSPFAYVD 587
Query: 546 PEAEVLTAIFTRLLVDYLNEYAYYAEVAGLYYGINLVESGFQVSLNGYNHKLRILLETIV 605
P + ++ LL D LNEYAY AE+AGL Y + G +S+ GYN K ILL+ I
Sbjct: 588 PLHCNMAYLYLELLKDSLNEYAYAAELAGLSYDLQNTIYGMYLSVKGYNDKQPILLKKIT 647
Query: 606 TKIANFSVKPDRFLVIKETLLKNYQNFKFQQPYQQAFYYCSLILGDRTWPLMDKLAILSN 665
K+A F + RF +IKE +++ NF+ +QP+Q A YY L++ + W + L +
Sbjct: 648 EKMATFEIDKKRFEIIKEAYMRSLNNFRAEQPHQHAMYYLRLLMTEVAWTKDELKEALDD 707
Query: 666 LGAQDLDKFVPTLLSSAYLECFIAGNIERTEAESMIDHIEDKFFKGSNPISRPLHPSQYP 725
+ L F+P LLS ++E + GNI + A ++ +ED + ++ ++PL PSQ
Sbjct: 708 VTLPRLKAFIPQLLSRLHIEALLHGNITKQAALGVMQMVEDTLIEHAH--TKPLLPSQLV 767
Query: 726 PNRIVKLERSIGYFYSAEGLNSNNENSALVHYIQVHRDEFLQNVKLQLFAHVAQQAAFHQ 785
R V+L + Y N + N + Y Q +N+ L+LF + + F+
Sbjct: 768 RYREVQLPDRGWFVYQRR--NEVHNNCGIEIYYQTDMQSTSENMFLELFCQIISEPCFNT 827
Query: 786 LRSVEQLGYITALAQRNDCGIRGVQFIIQSTVKGPRDIDLRVEAFLEMFEKKLVEMTVDE 845
LR+ EQLGYI R GI+G++FIIQS K P ++ RVEAFL EK + +MT +
Sbjct: 828 LRTKEQLGYIVFSGPRRANGIQGLRFIIQSE-KPPHYLESRVEAFLITMEKAIEDMTEEA 887
Query: 846 FQSNVNALVDAKLEKFKNLREEARFYWGEISEGTLKFDRRESEVAILKTLTHQDLINFFN 905
FQ ++ AL +L+K K L E YWGEI +DR EVA LKTL+ D+I F+
Sbjct: 888 FQKHIQALAIRRLDKPKKLSAECAKYWGEIISQQYNYDRDNIEVAYLKTLSKDDIIKFYK 947
Query: 906 EHIKVGAPRKKSLSVR----------VYGNLHSKEYSGDLNQPVQPNTVKIDDIFSFRRS 958
E + V APR+ +SV V G S+ P P I ++ F+R
Sbjct: 948 EMLAVDAPRRHKVSVHVLAREMDSCPVVGEFPSQNDINLSEAPPLPQPEVIHNMTEFKRG 1005
BLAST of Cucsa.337080 vs. TrEMBL
Match:
A0A061EXW9_THECC (Insulinase (Peptidase family M16) family protein isoform 1 OS=Theobroma cacao GN=TCM_024646 PE=3 SV=1)
HSP 1 Score: 1491.9 bits (3861), Expect = 0.0e+00
Identity = 732/967 (75.70%), Postives = 834/967 (86.25%), Query Frame = 1
Query: 1 MAVGKEQMTHEIVKARNDKREYKRIVLKNSLEVLLVSDPDTDKCAASMTVDVGSFSDPEG 60
MAVGKE + EI+K R DKREY+RIVL+NSL+VLLVSDPDTDKCAASM V VGSF DP G
Sbjct: 1 MAVGKEDV--EIIKPRTDKREYRRIVLRNSLQVLLVSDPDTDKCAASMNVGVGSFCDPVG 60
Query: 61 LEGLAHFLEHMLFYASEKYPQEDSYSKYITEHGGSTNAFTASEETNYYFDVNADCFEEAL 120
LEGLAHFLEHMLFYASEKYP EDSYSKYITEHGGSTNAFTASE+TNYYFDVN DCFEEAL
Sbjct: 61 LEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSTNAFTASEQTNYYFDVNTDCFEEAL 120
Query: 121 DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDVWRMHQLQCHISSESHPFHKFSTGN 180
DRFAQFFIKPLMS DAT REIKAVDSENQKNLLSD WRM+QLQ H+SSESHP+HKFSTGN
Sbjct: 121 DRFAQFFIKPLMSADATTREIKAVDSENQKNLLSDAWRMNQLQKHLSSESHPYHKFSTGN 180
Query: 181 WDTLEVQPKAKGLDTRHELLKFYENNYSSNVMHLVVYAKENLDKVQILVENIFQDIPNHN 240
W TLEV+PKAKG+DTR ELLKFYE+NYS+N+MHLVVYAKE+LDKVQ LVE+ FQ+I N +
Sbjct: 181 WKTLEVRPKAKGVDTRQELLKFYEDNYSANLMHLVVYAKESLDKVQSLVEDKFQEIRNSD 240
Query: 241 CNRANFPGQPCTSEHLQVLVRAIPIKEGHKLRIIWPITPEIHHHKEGPCRYLGHLIGHEG 300
+ F GQPCTSEHLQ+LVRA+PIK+GHKLRIIWPI P I +KEGPCRYLGHLIGHEG
Sbjct: 241 RSCFLFRGQPCTSEHLQILVRAVPIKQGHKLRIIWPIPPSIRLYKEGPCRYLGHLIGHEG 300
Query: 301 EGSLYYVLKTLGWATWLSADESSFTMNLSFFEVVIDLTDVGQEHMQDVIGLLFKYISLLK 360
EGSL+YVLKTLGWAT LSA E +T+ SFF+VVIDLTD G +HMQD++GLLFKY+ LL+
Sbjct: 301 EGSLFYVLKTLGWATGLSAGEGEWTLEFSFFKVVIDLTDAGHDHMQDIVGLLFKYVQLLQ 360
Query: 361 QSGIWQWIFDELSAICETKFHYADKIDPIDYVVNLSSSMQLYPPEDWLVGSSLPSKFNPK 420
QSG+ +WIFDELSA+CET FHY DK PIDYVVN++S+MQ+YPP+DWLVGSSLPS FNP
Sbjct: 361 QSGVCEWIFDELSAVCETGFHYQDKFPPIDYVVNIASNMQIYPPKDWLVGSSLPSNFNPD 420
Query: 421 LIGTVLDQLSVDNVRIFWESKKFEGKTDKVEKWYGTAYSIEKVSGPLVQDWMQSAPDVKL 480
I +L++L +NVRIFWES+KFEG TDKVE WYGTAYSIEKV+ +VQ+WM AP KL
Sbjct: 421 TIQMILNELCPENVRIFWESQKFEGLTDKVEPWYGTAYSIEKVTPSIVQEWMSLAPMEKL 480
Query: 481 HLPASNIFIPTDLSPKCACEKVKFPVLLKKSSYSSIWYKPDTMFSTPKAYVKIDFICPHA 540
HLPA N+FIPTDLS K + EKVKFPVLL+KSSYS +WYKPDTMFSTPKAYVKIDF CP+A
Sbjct: 481 HLPAPNVFIPTDLSLKSSQEKVKFPVLLRKSSYSKLWYKPDTMFSTPKAYVKIDFNCPYA 540
Query: 541 DISPEAEVLTAIFTRLLVDYLNEYAYYAEVAGLYYGINLVESGFQVSLNGYNHKLRILLE 600
SPEAEVL IF RLL+DYLNEYAYYA+VAGLYYGI +SGF+V+L GYNHKLRILLE
Sbjct: 541 SNSPEAEVLADIFARLLMDYLNEYAYYAQVAGLYYGIIHTDSGFEVTLVGYNHKLRILLE 600
Query: 601 TIVTKIANFSVKPDRFLVIKETLLKNYQNFKFQQPYQQAFYYCSLILGDRTWPLMDKLAI 660
T+V KIA F VKPDRF VIKE ++K+YQNFKFQQPYQQA Y CSLIL D+TWP M++L +
Sbjct: 601 TVVDKIAKFEVKPDRFSVIKEMVMKDYQNFKFQQPYQQAMYNCSLILEDQTWPWMEQLEV 660
Query: 661 LSNLGAQDLDKFVPTLLSSAYLECFIAGNIERTEAESMIDHIEDKFFKGSNPISRPLHPS 720
L +L A+DL KF +LS A+LEC+IAGNIE+ EAESMI +ED FFKGS PI +PL S
Sbjct: 661 LPHLNAEDLAKFATMMLSRAFLECYIAGNIEQEEAESMIQLVEDVFFKGSKPICQPLFLS 720
Query: 721 QYPPNRIVKLERSIGYFYSAEGLNSNNENSALVHYIQVHRDEFLQNVKLQLFAHVAQQAA 780
Q+ NR+VKLER + YFYS EGLN ++ENSALVHYIQVHRD+F+ NVKLQLFA +A+Q A
Sbjct: 721 QHLTNRVVKLERGMNYFYSKEGLNPSDENSALVHYIQVHRDDFILNVKLQLFALIAKQPA 780
Query: 781 FHQLRSVEQLGYITALAQRNDCGIRGVQFIIQSTVKGPRDIDLRVEAFLEMFEKKLVEMT 840
FHQLRSVEQLGYIT L QRND GIRGVQFIIQSTVKGP IDLRVEAFL MFE KL EMT
Sbjct: 781 FHQLRSVEQLGYITVLMQRNDSGIRGVQFIIQSTVKGPGHIDLRVEAFLRMFESKLYEMT 840
Query: 841 VDEFQSNVNALVDAKLEKFKNLREEARFYWGEISEGTLKFDRRESEVAILKTLTHQDLIN 900
DEF+SN+NAL+D KLEK KNLREE+RFYW EIS+GTLKFDRRE+EVA L+ LT Q+LI+
Sbjct: 841 NDEFKSNINALIDMKLEKHKNLREESRFYWREISDGTLKFDRREAEVAALRQLTQQELID 900
Query: 901 FFNEHIKVGAPRKKSLSVRVYGNLHSKEYSGDLNQPVQPNTVKIDDIFSFRRSQPLYGSF 960
FFNE+IKVGA +KK+LSVRVYGN H E + D ++P QP+T++IDDIFSFRRSQPLYGSF
Sbjct: 901 FFNENIKVGATQKKTLSVRVYGNQHLSEINSDKSEPSQPHTIQIDDIFSFRRSQPLYGSF 960
Query: 961 KYGNVKL 968
K G +KL
Sbjct: 961 KGGFMKL 965
BLAST of Cucsa.337080 vs. TrEMBL
Match:
D7THW7_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_08s0007g08490 PE=3 SV=1)
HSP 1 Score: 1481.5 bits (3834), Expect = 0.0e+00
Identity = 719/959 (74.97%), Postives = 820/959 (85.51%), Query Frame = 1
Query: 11 EIVKARNDKREYKRIVLKNSLEVLLVSDPDTDKCAASMTVDVGSFSDPEGLEGLAHFLEH 70
EIVK R D REY+RIVL+NSLEVLL+SDPDTDK AASM+V VGSF DPEG GLAHFLEH
Sbjct: 7 EIVKPRTDTREYRRIVLRNSLEVLLISDPDTDKAAASMSVSVGSFCDPEGFPGLAHFLEH 66
Query: 71 MLFYASEKYPQEDSYSKYITEHGGSTNAFTASEETNYYFDVNADCFEEALDRFAQFFIKP 130
MLFYASEKYP EDSYSKYI EHGGSTNAFT+SE TNYYFDVN+DCFEEALDRFAQFF+KP
Sbjct: 67 MLFYASEKYPLEDSYSKYIIEHGGSTNAFTSSEHTNYYFDVNSDCFEEALDRFAQFFVKP 126
Query: 131 LMSPDATMREIKAVDSENQKNLLSDVWRMHQLQCHISSESHPFHKFSTGNWDTLEVQPKA 190
LMS DAT REIKAVDSENQKNLLSD WRM QLQ HIS+E HP+HKFSTGNWDTLEV+PK
Sbjct: 127 LMSADATTREIKAVDSENQKNLLSDAWRMCQLQKHISAEGHPYHKFSTGNWDTLEVKPKE 186
Query: 191 KGLDTRHELLKFYENNYSSNVMHLVVYAKENLDKVQILVENIFQDIPNHNCNRANFPGQP 250
KGLDTRHEL+KFYE +YS+N+MHLVVY KE+LDK+Q LVE+ FQ+I N + + PGQP
Sbjct: 187 KGLDTRHELIKFYEEHYSANLMHLVVYTKESLDKIQSLVEHKFQEIQNKDRSNFQIPGQP 246
Query: 251 CTSEHLQVLVRAIPIKEGHKLRIIWPITPEIHHHKEGPCRYLGHLIGHEGEGSLYYVLKT 310
CTSEHLQ+LV+ +PIK+GHKLR+IWPITP IH++KEGPCRYLGHLIGHEGEGSL+Y+LKT
Sbjct: 247 CTSEHLQILVKTVPIKQGHKLRVIWPITPSIHNYKEGPCRYLGHLIGHEGEGSLFYILKT 306
Query: 311 LGWATWLSADESSFTMNLSFFEVVIDLTDVGQEHMQDVIGLLFKYISLLKQSGIWQWIFD 370
LGWAT LSA E +T SFF+VVIDLT+ G EHMQD++GLLFKYISLL+Q+G+ +WIFD
Sbjct: 307 LGWATSLSAGEGDWTCEFSFFKVVIDLTEAGHEHMQDIVGLLFKYISLLQQTGVCKWIFD 366
Query: 371 ELSAICETKFHYADKIDPIDYVVNLSSSMQLYPPEDWLVGSSLPSKFNPKLIGTVLDQLS 430
ELSAICET FHY DKI PIDYVVN+SS+M+LYPP+DWLVGSSLPSKF+P +I VLD+L+
Sbjct: 367 ELSAICETVFHYQDKIPPIDYVVNVSSNMELYPPKDWLVGSSLPSKFSPDVIQKVLDELA 426
Query: 431 VDNVRIFWESKKFEGKTDKVEKWYGTAYSIEKVSGPLVQDWMQSAPDVKLHLPASNIFIP 490
+NVRIFWESK FEG TD VE WYGTAYSIEK++ ++Q WM +AP+ LHLP+ N+FIP
Sbjct: 427 PNNVRIFWESKNFEGHTDMVEPWYGTAYSIEKITSSMIQQWMLAAPNEHLHLPSPNVFIP 486
Query: 491 TDLSPKCACEKVKFPVLLKKSSYSSIWYKPDTMFSTPKAYVKIDFICPHADISPEAEVLT 550
TDLS K EK KFPVLL+KSSYS++WYKPDTMFSTPKAYVKIDF CP A SPEA+VLT
Sbjct: 487 TDLSLKDVQEKAKFPVLLRKSSYSTLWYKPDTMFSTPKAYVKIDFNCPFASSSPEADVLT 546
Query: 551 AIFTRLLVDYLNEYAYYAEVAGLYYGINLVESGFQVSLNGYNHKLRILLETIVTKIANFS 610
IFTRLL+DYLNEYAYYA+VAGLYYGIN +SGFQV++ GYNHKLRILLET+V KIANF
Sbjct: 547 DIFTRLLMDYLNEYAYYAQVAGLYYGINHTDSGFQVAVTGYNHKLRILLETVVEKIANFK 606
Query: 611 VKPDRFLVIKETLLKNYQNFKFQQPYQQAFYYCSLILGDRTWPLMDKLAILSNLGAQDLD 670
VKPDRFLVIKE + K YQNFKFQQPYQQA YYCSLIL D TWP MD L ++ +L A DL
Sbjct: 607 VKPDRFLVIKEMVTKEYQNFKFQQPYQQAMYYCSLILQDNTWPWMDGLEVIPHLEADDLA 666
Query: 671 KFVPTLLSSAYLECFIAGNIERTEAESMIDHIEDKFFKGSNPISRPLHPSQYPPNRIVKL 730
KFVP LLS A+L+C+IAGNIE EAESMI HIED F+ G +PIS+PL PSQY NR++KL
Sbjct: 667 KFVPMLLSRAFLDCYIAGNIEPKEAESMIHHIEDIFYSGPHPISQPLFPSQYLTNRVIKL 726
Query: 731 ERSIGYFYSAEGLNSNNENSALVHYIQVHRDEFLQNVKLQLFAHVAQQAAFHQLRSVEQL 790
+R + YFY AEGLN ++ENSALVHYIQVHRD+FL NVKLQLFA +A+Q AFHQLRSVEQL
Sbjct: 727 DRGMSYFYPAEGLNPSDENSALVHYIQVHRDDFLPNVKLQLFALIAKQRAFHQLRSVEQL 786
Query: 791 GYITALAQRNDCGIRGVQFIIQSTVKGPRDIDLRVEAFLEMFEKKLVEMTVDEFQSNVNA 850
GYIT L QRND GIRGVQFIIQSTVKGP ID RV FL+MFE KL M+ DEF+SNVNA
Sbjct: 787 GYITVLMQRNDSGIRGVQFIIQSTVKGPGHIDSRVVEFLKMFESKLYAMSEDEFKSNVNA 846
Query: 851 LVDAKLEKFKNLREEARFYWGEISEGTLKFDRRESEVAILKTLTHQDLINFFNEHIKVGA 910
L+D KLEK KNLREE+ FYW EI +GTLKFDRRE+EVA LK LT ++LI+FFNEHIKVGA
Sbjct: 847 LIDMKLEKHKNLREESGFYWREIYDGTLKFDRREAEVAALKKLTQKELIDFFNEHIKVGA 906
Query: 911 PRKKSLSVRVYGNLHSKEYSGDLNQPVQPNTVKIDDIFSFRRSQPLYGSFK--YGNVKL 968
P+KK+LSVRVYG LH+ EY+ + + QP VKIDDIF FR+SQPLYGSFK G VKL
Sbjct: 907 PQKKTLSVRVYGGLHTSEYADEKKEANQPKQVKIDDIFKFRKSQPLYGSFKGGLGQVKL 965
BLAST of Cucsa.337080 vs. TrEMBL
Match:
A0A0D2Q6F9_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_002G147300 PE=3 SV=1)
HSP 1 Score: 1476.8 bits (3822), Expect = 0.0e+00
Identity = 723/969 (74.61%), Postives = 828/969 (85.45%), Query Frame = 1
Query: 1 MAVGKEQMTHEIVKARNDKREYKRIVLKNSLEVLLVSDPDTDKCAASMTVDVGSFSDPEG 60
MAVG+E + EI+K R DKREY+RIVL+NSL+VLL+SD DTDKCAASM V VGSF DP+G
Sbjct: 1 MAVGREDV--EILKPRIDKREYRRIVLRNSLQVLLISDLDTDKCAASMNVGVGSFCDPDG 60
Query: 61 LEGLAHFLEHMLFYASEKYPQEDSYSKYITEHGGSTNAFTASEETNYYFDVNADCFEEAL 120
LEGLAHFLEHMLFYASEKYP EDSYSKYITEHGGSTNAFTASE TNYYFDVN DCFEEAL
Sbjct: 61 LEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSTNAFTASEMTNYYFDVNTDCFEEAL 120
Query: 121 DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDVWRMHQLQCHISSESHPFHKFSTGN 180
DRFAQFFIKPLMS DATMREIKAVDSENQKNLLSD WRM+QLQ H+S ESHP+HKFSTGN
Sbjct: 121 DRFAQFFIKPLMSADATMREIKAVDSENQKNLLSDAWRMNQLQKHLSLESHPYHKFSTGN 180
Query: 181 WDTLEVQPKAKGLDTRHELLKFYENNYSSNVMHLVVYAKENLDKVQILVENIFQDIPNHN 240
WDTL+V+PKAKG+DTR ELLKFYE+ YS+N+MHLVVY+KE+LDK+Q LVE+ FQ+I N +
Sbjct: 181 WDTLDVRPKAKGVDTRQELLKFYEDKYSANLMHLVVYSKESLDKIQCLVEDKFQEIQNSD 240
Query: 241 CNRANFPGQPCTSEHLQVLVRAIPIKEGHKLRIIWPITPEIHHHKEGPCRYLGHLIGHEG 300
+R FPGQPCTSEHL++LVRA+PIK+GHKLRI+WPITP I H+KEGPCRYLGHLIGHEG
Sbjct: 241 RSRFQFPGQPCTSEHLEILVRAVPIKQGHKLRIVWPITPSILHYKEGPCRYLGHLIGHEG 300
Query: 301 EGSLYYVLKTLGWATWLSADESSFTMNLSFFEVVIDLTDVGQEHMQDVIGLLFKYISLLK 360
EGSL+YVLK GWAT LSA E +T SFF VVIDLTD GQ++MQD++GLLFKYI LL+
Sbjct: 301 EGSLFYVLKKSGWATGLSAGEGEWTSEFSFFNVVIDLTDAGQDNMQDIVGLLFKYIQLLQ 360
Query: 361 QSGIWQWIFDELSAICETKFHYADKIDPIDYVVNLSSSMQLYPPEDWLVGSSLPSKFNPK 420
QSG+ +WIFDELSA+CET FHY DKI PIDYVVN+SS+MQ+YPP+DWLVGS LPS FNP
Sbjct: 361 QSGVCKWIFDELSAVCETGFHYQDKISPIDYVVNISSNMQIYPPKDWLVGSLLPSDFNPA 420
Query: 421 LIGTVLDQLSVDNVRIFWESKKFEGKTDKVEKWYGTAYSIEKVSGPLVQDWMQSAPDVKL 480
+I +L++LS +NVRIFWESKKFEG TDKVE WYGTAYSIEKVS +Q WM SAP+ L
Sbjct: 421 IIQKILNELSPENVRIFWESKKFEGLTDKVEPWYGTAYSIEKVSSSKIQAWMSSAPNENL 480
Query: 481 HLPASNIFIPTDLSPKCACEKVKFPVLLKKSSYSSIWYKPDTMFSTPKAYVKIDFICPHA 540
HLPA N+FIP DLS K A E+VKFPVLL+KSSYS +WYKPDT+FSTPKAYVKIDF CPHA
Sbjct: 481 HLPAPNVFIPKDLSIKNAQEEVKFPVLLRKSSYSKLWYKPDTVFSTPKAYVKIDFNCPHA 540
Query: 541 DISPEAEVLTAIFTRLLVDYLNEYAYYAEVAGLYYGINLVESGFQVSLNGYNHKLRILLE 600
SPE EVL +F RLL+DYLNEYAYYA+VAGL YGI+ +SGF+V+L GYNHKLRILLE
Sbjct: 541 GNSPETEVLGDLFARLLLDYLNEYAYYAQVAGLLYGISHTDSGFEVTLVGYNHKLRILLE 600
Query: 601 TIVTKIANFSVKPDRFLVIKETLLKNYQNFKFQQPYQQAFYYCSLILGDRTWPLMDKLAI 660
TI+ KI F VKPDRF VIKE +K+YQNFKFQQPYQQA YYCSLIL D+T P +++L +
Sbjct: 601 TIIDKIVKFEVKPDRFSVIKEMEIKDYQNFKFQQPYQQAMYYCSLILKDQTRPWVERLDV 660
Query: 661 LSNLGAQDLDKFVPTLLSSAYLECFIAGNIERTEAESMIDHIEDKFFKGSNPISRPLHPS 720
L L +DL F P +LS A+LEC+IAGNIER EAESM+ H+ED FFKG NPI RPL PS
Sbjct: 661 LPRLNVEDLTNFAPMMLSQAFLECYIAGNIEREEAESMVQHVEDVFFKGPNPICRPLFPS 720
Query: 721 QYPPNRIVKLERSIGYFYSAEGLNSNNENSALVHYIQVHRDEFLQNVKLQLFAHVAQQAA 780
Q+ NR+VKLER + Y YS EGLN ++ENSALVHYIQVH+D+F+ NVKLQLFA VA+Q A
Sbjct: 721 QFLTNRVVKLERGMNYCYSKEGLNPSDENSALVHYIQVHQDDFILNVKLQLFALVAKQPA 780
Query: 781 FHQLRSVEQLGYITALAQRNDCGIRGVQFIIQSTVKGPRDIDLRVEAFLEMFEKKLVEMT 840
FHQLRSVEQLGYIT L QRND GI GVQFIIQSTVKGP ID RVEAFL+MFE KL EMT
Sbjct: 781 FHQLRSVEQLGYITVLMQRNDSGICGVQFIIQSTVKGPAHIDSRVEAFLKMFENKLYEMT 840
Query: 841 VDEFQSNVNALVDAKLEKFKNLREEARFYWGEISEGTLKFDRRESEVAILKTLTHQDLIN 900
DEF+SNVNAL+D KLEK KNLREE+RFYW EI++GTLKFDRRE+EVA LK LT Q+LI
Sbjct: 841 NDEFKSNVNALIDMKLEKHKNLREESRFYWREITDGTLKFDRREAEVAALKKLTQQELIE 900
Query: 901 FFNEHIKVGAPRKKSLSVRVYGNLHSKEYSGDLNQPVQPNTVKIDDIFSFRRSQPLYGSF 960
FFNE++KVGA RKK+LSVRV+GN H EY ++ VQPNT++I+DIFSFRRSQPLYGSF
Sbjct: 901 FFNENVKVGATRKKTLSVRVHGNQHLAEYHSQKSEAVQPNTIQINDIFSFRRSQPLYGSF 960
Query: 961 K--YGNVKL 968
+ G+VKL
Sbjct: 961 RGGIGHVKL 967
BLAST of Cucsa.337080 vs. TrEMBL
Match:
A0A067JQ91_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_21090 PE=3 SV=1)
HSP 1 Score: 1474.9 bits (3817), Expect = 0.0e+00
Identity = 709/969 (73.17%), Postives = 836/969 (86.27%), Query Frame = 1
Query: 1 MAVGKEQMTHEIVKARNDKREYKRIVLKNSLEVLLVSDPDTDKCAASMTVDVGSFSDPEG 60
MAVGKE++ EIVK R D REY+RIVLKNSL+VLL+SDP+TDKCAASM V VGSFSDP G
Sbjct: 1 MAVGKEEV--EIVKPRTDTREYRRIVLKNSLKVLLISDPETDKCAASMNVSVGSFSDPVG 60
Query: 61 LEGLAHFLEHMLFYASEKYPQEDSYSKYITEHGGSTNAFTASEETNYYFDVNADCFEEAL 120
LEGLAHFLEHMLFYASEKYP EDSYSKYITEHGGSTNAFT+S+ TNYYFDVN DCFE+AL
Sbjct: 61 LEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSTNAFTSSKHTNYYFDVNTDCFEDAL 120
Query: 121 DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDVWRMHQLQCHISSESHPFHKFSTGN 180
DRFAQFFIKPLMS DATMREIKAVDSENQKNLLSD WRM+QLQ H+S + HP+HKFSTGN
Sbjct: 121 DRFAQFFIKPLMSADATMREIKAVDSENQKNLLSDAWRMNQLQKHLSDKGHPYHKFSTGN 180
Query: 181 WDTLEVQPKAKGLDTRHELLKFYENNYSSNVMHLVVYAKENLDKVQILVENIFQDIPNHN 240
WDTLEV+PKAKGLDTRHEL+KFYE +YS+N+MHLV+YAKE+LDK+Q V++ FQ+I N++
Sbjct: 181 WDTLEVRPKAKGLDTRHELIKFYEEHYSANLMHLVIYAKESLDKIQSFVKDKFQEIRNND 240
Query: 241 CNRANFPGQPCTSEHLQVLVRAIPIKEGHKLRIIWPITPEIHHHKEGPCRYLGHLIGHEG 300
+ +FPGQPCTSEHLQ+LVRA+PIK+GHKL+IIWPITP I H+KEGPCRYLGHLIGHEG
Sbjct: 241 RSCLSFPGQPCTSEHLQILVRAVPIKQGHKLKIIWPITPGILHYKEGPCRYLGHLIGHEG 300
Query: 301 EGSLYYVLKTLGWATWLSADESSFTMNLSFFEVVIDLTDVGQEHMQDVIGLLFKYISLLK 360
EGSLY+VLKTLGWAT L+A E +T SFF+V+IDLTD G EHMQ+++GLLFKYI LL+
Sbjct: 301 EGSLYFVLKTLGWATSLAAGEGDWTTEFSFFKVLIDLTDAGHEHMQEIVGLLFKYIHLLQ 360
Query: 361 QSGIWQWIFDELSAICETKFHYADKIDPIDYVVNLSSSMQLYPPEDWLVGSSLPSKFNPK 420
QSG+ +WIFDEL+A+CET FHY DK PIDYVV +S +M +YPP+DWLVGSSLPS F+P
Sbjct: 361 QSGVCKWIFDELTAVCETAFHYQDKTPPIDYVVKISCNMGMYPPKDWLVGSSLPSNFSPS 420
Query: 421 LIGTVLDQLSVDNVRIFWESKKFEGKTDKVEKWYGTAYSIEKVSGPLVQDWMQSAPDVKL 480
I + DQLS +NVRIFWESKKFEG+T+ VE+WYGTAYS+EK++ L+Q+WM SAP+ L
Sbjct: 421 TIQMIFDQLSPENVRIFWESKKFEGQTEMVEQWYGTAYSVEKITSSLIQEWMLSAPNENL 480
Query: 481 HLPASNIFIPTDLSPKCACEKVKFPVLLKKSSYSSIWYKPDTMFSTPKAYVKIDFICPHA 540
HLPA N+FIPTDLS K A EKVKFPVLL+KSSYSS+W+KPDTMFSTPKAYVKIDF CPH
Sbjct: 481 HLPAPNVFIPTDLSLKNAQEKVKFPVLLRKSSYSSLWFKPDTMFSTPKAYVKIDFSCPHG 540
Query: 541 DISPEAEVLTAIFTRLLVDYLNEYAYYAEVAGLYYGINLVESGFQVSLNGYNHKLRILLE 600
ISPEA+VLT +FTRL++DYLNE+AYYAEVAGL YGI + GFQV++ GYNHKLRILLE
Sbjct: 541 GISPEAKVLTGLFTRLVMDYLNEFAYYAEVAGLSYGITNTDGGFQVTVVGYNHKLRILLE 600
Query: 601 TIVTKIANFSVKPDRFLVIKETLLKNYQNFKFQQPYQQAFYYCSLILGDRTWPLMDKLAI 660
T++ KIA F V PDRF VIKE ++K Y+N KFQQPYQQA Y+CSLIL ++ WP M+++ +
Sbjct: 601 TVMEKIAKFEVNPDRFPVIKEMVIKEYENLKFQQPYQQAMYHCSLILENQGWPWMEQIEV 660
Query: 661 LSNLGAQDLDKFVPTLLSSAYLECFIAGNIERTEAESMIDHIEDKFFKGSNPISRPLHPS 720
L L A+DL KFVPTLLS A+LEC+IAGNIER+EAE +I+H+ED F+KGSNPI + L PS
Sbjct: 661 LHRLEAEDLSKFVPTLLSRAFLECYIAGNIERSEAEKIIEHVEDVFYKGSNPICQALFPS 720
Query: 721 QYPPNRIVKLERSIGYFYSAEGLNSNNENSALVHYIQVHRDEFLQNVKLQLFAHVAQQAA 780
Q+ NR++KLE+ Y Y EGLN ++ENSALVHYIQVHRD+F+ NVKLQLFA +A+Q A
Sbjct: 721 QHLTNRVIKLEKGKNYLYPIEGLNPSDENSALVHYIQVHRDDFMLNVKLQLFALIAKQPA 780
Query: 781 FHQLRSVEQLGYITALAQRNDCGIRGVQFIIQSTVKGPRDIDLRVEAFLEMFEKKLVEMT 840
FHQLRSVEQLGYIT L RND GI GVQFIIQSTVKGP IDLRVEAFL+MFE KL EMT
Sbjct: 781 FHQLRSVEQLGYITVLMPRNDSGICGVQFIIQSTVKGPGQIDLRVEAFLKMFETKLYEMT 840
Query: 841 VDEFQSNVNALVDAKLEKFKNLREEARFYWGEISEGTLKFDRRESEVAILKTLTHQDLIN 900
DEF++NVNAL+D KLEK KNLREE+RFYW EI +GTLKFDRR+SEVA L+ LT ++ I
Sbjct: 841 NDEFKNNVNALIDMKLEKHKNLREESRFYWREIDDGTLKFDRRDSEVAALRQLTQKEFIE 900
Query: 901 FFNEHIKVGAPRKKSLSVRVYGNLHSKEYSGDLNQPVQPNTVKIDDIFSFRRSQPLYGSF 960
FFNE+IKVGAP+K++LSVRVYG LHS EY+ D ++ V PN+V+IDDIFSF+RSQPLYGSF
Sbjct: 901 FFNENIKVGAPQKRTLSVRVYGGLHSSEYTSDKSEAVPPNSVQIDDIFSFKRSQPLYGSF 960
Query: 961 K--YGNVKL 968
K +G+VKL
Sbjct: 961 KGGFGHVKL 967
BLAST of Cucsa.337080 vs. TrEMBL
Match:
M5VTN9_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa000903mg PE=3 SV=1)
HSP 1 Score: 1451.4 bits (3756), Expect = 0.0e+00
Identity = 708/967 (73.22%), Postives = 817/967 (84.49%), Query Frame = 1
Query: 1 MAVGKEQMTHEIVKARNDKREYKRIVLKNSLEVLLVSDPDTDKCAASMTVDVGSFSDPEG 60
MAVGKE++ EIVKAR DKREY+RIVL NSLEVLL+SDPDTDKCAASM V VG+FSDP+G
Sbjct: 1 MAVGKEEV-EEIVKARTDKREYRRIVLPNSLEVLLISDPDTDKCAASMDVSVGAFSDPDG 60
Query: 61 LEGLAHFLEHMLFYASEKYPQEDSYSKYITEHGGSTNAFTASEETNYYFDVNADCFEEAL 120
LEGLAHFLEHMLFYASEKYP EDSYSKYITEHGG TNA+T+SE TNY+FD+NAD FEEAL
Sbjct: 61 LEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGRTNAYTSSEHTNYHFDINADAFEEAL 120
Query: 121 DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDVWRMHQLQCHISSESHPFHKFSTGN 180
DRFAQFFI PLMS DATMREIKAVDSENQKNLLSD WRM+QLQ H+S+ HP+HKFSTGN
Sbjct: 121 DRFAQFFINPLMSADATMREIKAVDSENQKNLLSDGWRMNQLQKHLSAVDHPYHKFSTGN 180
Query: 181 WDTLEVQPKAKGLDTRHELLKFYENNYSSNVMHLVVYAKENLDKVQILVENIFQDIPNHN 240
WDTLEV+PKAKGLDTR EL+KFY YS+NVMHLVVY KENLDK+Q LVE+ F++I N +
Sbjct: 181 WDTLEVRPKAKGLDTRSELIKFYAEYYSANVMHLVVYGKENLDKIQGLVEDKFKEIRNID 240
Query: 241 CNRANFPGQPCTSEHLQVLVRAIPIKEGHKLRIIWPITPEIHHHKEGPCRYLGHLIGHEG 300
N F G+PCTSEHLQ+LVRA+PIKEGH LR+ WPITPEIHH+KEGPCRYL HLIGHEG
Sbjct: 241 RNCPRFVGEPCTSEHLQILVRAVPIKEGHALRVAWPITPEIHHYKEGPCRYLSHLIGHEG 300
Query: 301 EGSLYYVLKTLGWATWLSADESSFTMNLSFFEVVIDLTDVGQEHMQDVIGLLFKYISLLK 360
EGSLYY+LKTLGWAT LSA E T + SFF + IDLTD G EHMQD+IGLLFKYISLL+
Sbjct: 301 EGSLYYILKTLGWATGLSAGEGESTFDFSFFRIDIDLTDAGHEHMQDIIGLLFKYISLLQ 360
Query: 361 QSGIWQWIFDELSAICETKFHYADKIDPIDYVVNLSSSMQLYPPEDWLVGSSLPSKFNPK 420
QSGI +WIFDELSA+CETKFHY DKI PI YVV++S +MQ YPP+DWLV SSLPS F+
Sbjct: 361 QSGICKWIFDELSAVCETKFHYQDKIQPISYVVSISPNMQKYPPKDWLVRSSLPSNFSTD 420
Query: 421 LIGTVLDQLSVDNVRIFWESKKFEGKTDKVEKWYGTAYSIEKVSGPLVQDWMQSAPDVKL 480
+I VL++LS +NVRIFWESKKFEG+T+ VE WYGTAYSIEK++G ++Q+W+ S+P+ L
Sbjct: 421 IIQIVLNKLSPNNVRIFWESKKFEGQTNMVEPWYGTAYSIEKITGSMIQEWIVSSPNENL 480
Query: 481 HLPASNIFIPTDLSPKCACEKVKFPVLLKKSSYSSIWYKPDTMFSTPKAYVKIDFICPHA 540
HLPA N+FIPTDLS K EK K+PVLL+KS YS++W+KPDTMF TPKAYVKI F CPHA
Sbjct: 481 HLPAPNVFIPTDLSLKNDHEKAKYPVLLRKSPYSTLWHKPDTMFFTPKAYVKIVFTCPHA 540
Query: 541 DISPEAEVLTAIFTRLLVDYLNEYAYYAEVAGLYYGINLVESGFQVSLNGYNHKLRILLE 600
SPEAEVLT IFT+LL+DYLNE+AYYA+VAGL YGI+ +SGFQV L GYNHKLRILLE
Sbjct: 541 SDSPEAEVLTNIFTQLLMDYLNEFAYYAQVAGLNYGISHTDSGFQVILAGYNHKLRILLE 600
Query: 601 TIVTKIANFSVKPDRFLVIKETLLKNYQNFKFQQPYQQAFYYCSLILGDRTWPLMDKLAI 660
T+V KIA+F VK DRF VIKE + K YQN+KF+QPY+QA YYCSLIL D TWP M++L +
Sbjct: 601 TVVEKIASFEVKADRFSVIKEMVTKEYQNYKFRQPYEQAMYYCSLILQDHTWPWMEELDV 660
Query: 661 LSNLGAQDLDKFVPTLLSSAYLECFIAGNIERTEAESMIDHIEDKFFKGSNPISRPLHPS 720
L +L +DL KFVP +LS A+LEC+ AGN+ER EAESMI HIED FKGSNPI +PL PS
Sbjct: 661 LPHLEVEDLAKFVPMMLSRAFLECYTAGNLERNEAESMIQHIEDVLFKGSNPICQPLFPS 720
Query: 721 QYPPNRIVKLERSIGYFYSAEGLNSNNENSALVHYIQVHRDEFLQNVKLQLFAHVAQQAA 780
Q+ NR+VKLE+ YFY EGLN ++ENSAL+HYIQVHRD+F+ NVKL LFA +A+Q A
Sbjct: 721 QHLTNRVVKLEKGKSYFYPVEGLNPSDENSALIHYIQVHRDDFMLNVKLHLFALIAKQPA 780
Query: 781 FHQLRSVEQLGYITALAQRNDCGIRGVQFIIQSTVKGPRDIDLRVEAFLEMFEKKLVEMT 840
FHQLRSVEQLGYITAL QRNDCGIRG F+IQSTVK P IDLR E FL+ F+ KL EMT
Sbjct: 781 FHQLRSVEQLGYITALLQRNDCGIRGALFVIQSTVKDPAHIDLRAEEFLKAFKSKLYEMT 840
Query: 841 VDEFQSNVNALVDAKLEKFKNLREEARFYWGEISEGTLKFDRRESEVAILKTLTHQDLIN 900
+EF+SNVNAL+D KLEK KNLREEA FYW EIS+GTLKFDR ESE+A L+ LT Q+LI+
Sbjct: 841 NEEFKSNVNALIDMKLEKHKNLREEAAFYWREISDGTLKFDRIESEIAALRQLTQQELID 900
Query: 901 FFNEHIKVGAPRKKSLSVRVYGNLHSKEYSGDLNQPVQPNTVKIDDIFSFRRSQPLYGSF 960
FFNEHIKVGAP K++LSVRVYG HS EY D + P Q +++KIDDIFSFRRSQPLYGSF
Sbjct: 901 FFNEHIKVGAPHKRTLSVRVYGKSHSSEYKIDKSSPGQASSIKIDDIFSFRRSQPLYGSF 960
Query: 961 KYGNVKL 968
K +VKL
Sbjct: 961 KGNHVKL 966
BLAST of Cucsa.337080 vs. TAIR10
Match:
AT2G41790.1 (AT2G41790.1 Insulinase (Peptidase family M16) family protein)
HSP 1 Score: 1379.8 bits (3570), Expect = 0.0e+00
Identity = 665/964 (68.98%), Postives = 803/964 (83.30%), Query Frame = 1
Query: 1 MAVGKEQMTH---EIVKARNDKREYKRIVLKNSLEVLLVSDPDTDKCAASMTVDVGSFSD 60
MAV K T EI+K R D REY+ IVLKN L+VLL+SDPDTDKCAASM+V VGSFSD
Sbjct: 1 MAVEKSNTTVGGVEILKPRTDNREYRMIVLKNLLQVLLISDPDTDKCAASMSVSVGSFSD 60
Query: 61 PEGLEGLAHFLEHMLFYASEKYPQEDSYSKYITEHGGSTNAFTASEETNYYFDVNADCFE 120
P+GLEGLAHFLEHMLFYASEKYP+EDSYSKYITEHGGSTNA+TASEETNY+FDVNADCF+
Sbjct: 61 PQGLEGLAHFLEHMLFYASEKYPEEDSYSKYITEHGGSTNAYTASEETNYHFDVNADCFD 120
Query: 121 EALDRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDVWRMHQLQCHISSESHPFHKFS 180
EALDRFAQFFIKPLMS DATMREIKAVDSENQKNLLSD WR+ QLQ H+S E HP+HKFS
Sbjct: 121 EALDRFAQFFIKPLMSADATMREIKAVDSENQKNLLSDGWRIRQLQKHLSKEDHPYHKFS 180
Query: 181 TGNWDTLEVQPKAKGLDTRHELLKFYENNYSSNVMHLVVYAKENLDKVQILVENIFQDIP 240
TGN DTL V+P+AKG+DTR EL+KFYE +YS+N+MHLVVY KE+LDK+Q LVE +FQ+I
Sbjct: 181 TGNMDTLHVRPQAKGVDTRSELIKFYEEHYSANIMHLVVYGKESLDKIQDLVERMFQEIQ 240
Query: 241 NHNCNRANFPGQPCTSEHLQVLVRAIPIKEGHKLRIIWPITPEIHHHKEGPCRYLGHLIG 300
N N FPGQPCT++HLQ+LV+AIPIK+GHKL + WP+TP IHH+ E P +YLGHLIG
Sbjct: 241 NTNKVVPRFPGQPCTADHLQILVKAIPIKQGHKLGVSWPVTPSIHHYDEAPSQYLGHLIG 300
Query: 301 HEGEGSLYYVLKTLGWATWLSADESSFTMNLSFFEVVIDLTDVGQEHMQDVIGLLFKYIS 360
HEGEGSL++ LKTLGWAT LSA E +T++ SFF+V IDLTD G EHMQ+++GLLF YI
Sbjct: 301 HEGEGSLFHALKTLGWATGLSAGEGEWTLDYSFFKVSIDLTDAGHEHMQEILGLLFNYIQ 360
Query: 361 LLKQSGIWQWIFDELSAICETKFHYADKIDPIDYVVNLSSSMQLYPPEDWLVGSSLPSKF 420
LL+Q+G+ QWIFDELSAICETKFHY DKI P+ Y+V+++S+MQ+YP +DWLVGSSLP+KF
Sbjct: 361 LLQQTGVCQWIFDELSAICETKFHYQDKIPPMSYIVDIASNMQIYPTKDWLVGSSLPTKF 420
Query: 421 NPKLIGTVLDQLSVDNVRIFWESKKFEGKTDKVEKWYGTAYSIEKVSGPLVQDWMQSAPD 480
NP ++ V+D+LS N RIFWES+KFEG+TDK E WY TAYS+EK++ +Q+W+QSAPD
Sbjct: 421 NPAIVQKVVDELSPSNFRIFWESQKFEGQTDKAEPWYNTAYSLEKITSSTIQEWVQSAPD 480
Query: 481 VKLHLPASNIFIPTDLSPKCACEKVKFPVLLKKSSYSSIWYKPDTMFSTPKAYVKIDFIC 540
V LHLPA N+FIPTDLS K A +K PVLL+K+ +S +WYKPDTMFS PKAYVK+DF C
Sbjct: 481 VHLHLPAPNVFIPTDLSLKDADDKETVPVLLRKTPFSRLWYKPDTMFSKPKAYVKMDFNC 540
Query: 541 PHADISPEAEVLTAIFTRLLVDYLNEYAYYAEVAGLYYGINLVESGFQVSLNGYNHKLRI 600
P A SP+A VLT IFTRLL+DYLNEYAYYA+VAGLYYG++L ++GF+++L GYNHKLRI
Sbjct: 541 PLAVSSPDAAVLTDIFTRLLMDYLNEYAYYAQVAGLYYGVSLSDNGFELTLLGYNHKLRI 600
Query: 601 LLETIVTKIANFSVKPDRFLVIKETLLKNYQNFKFQQPYQQAFYYCSLILGDRTWPLMDK 660
LLET+V KIANF VKPDRF VIKET+ K YQN+KF+QPY QA YYCSLIL D+TWP ++
Sbjct: 601 LLETVVGKIANFEVKPDRFAVIKETVTKEYQNYKFRQPYHQAMYYCSLILQDQTWPWTEE 660
Query: 661 LAILSNLGAQDLDKFVPTLLSSAYLECFIAGNIERTEAESMIDHIEDKFFKGSNPISRPL 720
L +LS+L A+D+ KFVP LLS ++EC+IAGN+E EAESM+ HIED F PI RPL
Sbjct: 661 LDVLSHLEAEDVAKFVPMLLSRTFIECYIAGNVENNEAESMVKHIEDVLFNDPKPICRPL 720
Query: 721 HPSQYPPNRIVKLERSIGYFYSAEGLNSNNENSALVHYIQVHRDEFLQNVKLQLFAHVAQ 780
PSQ+ NR+VKL + YFY +G N ++ENSALVHYIQVHRD+F N+KLQLF VA+
Sbjct: 721 FPSQHLTNRVVKLGEGMKYFYHQDGSNPSDENSALVHYIQVHRDDFSMNIKLQLFGLVAK 780
Query: 781 QAAFHQLRSVEQLGYITALAQRNDCGIRGVQFIIQSTVKGPRDIDLRVEAFLEMFEKKLV 840
QA FHQLR+VEQLGYITALAQRND GI GVQFIIQS+VKGP ID RVE+ L+ FE KL
Sbjct: 781 QATFHQLRTVEQLGYITALAQRNDSGIYGVQFIIQSSVKGPGHIDSRVESLLKNFESKLY 840
Query: 841 EMTVDEFQSNVNALVDAKLEKFKNLREEARFYWGEISEGTLKFDRRESEVAILKTLTHQD 900
EM+ ++F+SNV AL+D KLEK KNL+EE+RFYW EI GTLKF+R+E+EV+ LK L Q+
Sbjct: 841 EMSNEDFKSNVTALIDMKLEKHKNLKEESRFYWREIQSGTLKFNRKEAEVSALKQLQKQE 900
Query: 901 LINFFNEHIKVGAPRKKSLSVRVYGNLHSKEYSGDLNQPVQPNTVKIDDIFSFRRSQPLY 960
LI+FF+E+IKVGA RKKSLS+RVYG+ H KE + D ++ P +V+I+DI FR+SQPL+
Sbjct: 901 LIDFFDEYIKVGAARKKSLSIRVYGSQHLKEMASDKDEVPSP-SVEIEDIVGFRKSQPLH 960
Query: 961 GSFK 962
GSF+
Sbjct: 961 GSFR 963
BLAST of Cucsa.337080 vs. TAIR10
Match:
AT3G57470.2 (AT3G57470.2 Insulinase (Peptidase family M16) family protein)
HSP 1 Score: 1176.0 bits (3041), Expect = 0.0e+00
Identity = 573/891 (64.31%), Postives = 702/891 (78.79%), Query Frame = 1
Query: 71 MLFYASEKYPQEDSYSKYITEHGGSTNAFTASEETNYYFDVNADCFEEALDRFAQFFIKP 130
MLFYASEKYP+EDSYSKYITEHGGSTNA+T+SE+TNY+FD+N D F EALDRFAQFFI+P
Sbjct: 1 MLFYASEKYPEEDSYSKYITEHGGSTNAYTSSEDTNYHFDINTDSFYEALDRFAQFFIQP 60
Query: 131 LMSPDATMREIKAVDSENQKNLLSDVWRMHQLQCHISSESHPFHKFSTGNWDTLEVQPKA 190
LMS DATMREIKAVDSE+Q NLLSD WRM QLQ H+S E HP+HKFSTGN DTL V+P+
Sbjct: 61 LMSTDATMREIKAVDSEHQNNLLSDSWRMAQLQKHLSREDHPYHKFSTGNMDTLHVRPEE 120
Query: 191 KGLDTRHELLKFYENNYSSNVMHLVVYAKENLDKVQILVENIFQDIPNHNCNRANFPGQP 250
G+DTR EL+KFY+ +YS+N+MHLVVY KENLDK Q LVE +FQ I N N FPGQP
Sbjct: 121 NGVDTRSELIKFYDEHYSANIMHLVVYGKENLDKTQGLVEALFQGIRNTNQGIPRFPGQP 180
Query: 251 CTSEHLQVLVRAIPIKEGHKLRIIWPITPEIHHHKEGPCRYLGHLIGHEGEGSLYYVLKT 310
CT +HLQVLV+A+PI +GH+L + WP+TP I H++E PCRYLG LIGHEGEGSL++ LK
Sbjct: 181 CTLDHLQVLVKAVPIMQGHELSVSWPVTPSISHYEEAPCRYLGDLIGHEGEGSLFHALKI 240
Query: 311 LGWATWLSADESSFTMNLSFFEVVIDLTDVGQEHMQDVIGLLFKYISLLKQSGIWQWIFD 370
LGWAT L A E+ ++M SFF V IDLTD G EHMQD++GLLF+YI +L+QSG+ QWIFD
Sbjct: 241 LGWATGLYAGEADWSMEYSFFNVSIDLTDAGHEHMQDILGLLFEYIKVLQQSGVSQWIFD 300
Query: 371 ELSAICETKFHYADKIDPIDYVVNLSSSMQLYPPEDWLVGSSLPSKFNPKLIGTVLDQLS 430
ELSAICE +FHY KIDPI Y V++SS+M++YP + WLVGSSLPSKFNP ++ VLD+LS
Sbjct: 301 ELSAICEAEFHYQAKIDPISYAVDISSNMKIYPTKHWLVGSSLPSKFNPAIVQKVLDELS 360
Query: 431 VDNVRIFWESKKFEGKTDKVEKWYGTAYSIEKVSGPLVQDWMQSAPDVKLHLPASNIFIP 490
+NVRIFWES KFEG+TDKVE WY TAYS+EK++ +Q+WMQSAPDV L LP N+FIP
Sbjct: 361 PNNVRIFWESNKFEGQTDKVEPWYNTAYSLEKITKFTIQEWMQSAPDVNLLLPTPNVFIP 420
Query: 491 TDLSPKCACEKVKFPVLLKKSSYSSIWYKPDTMFSTPKAYVKIDFICPHADISPEAEVLT 550
TD S K +K FPVLL+K+SYS +WYKPDT F PKAYVK+DF CP A SP+A VL+
Sbjct: 421 TDFSLKDLKDKDIFPVLLRKTSYSRLWYKPDTKFFKPKAYVKMDFNCPLAVSSPDAAVLS 480
Query: 551 AIFTRLLVDYLNEYAYYAEVAGLYYGINLVESGFQVSLNGYNHKLRILLETIVTKIANFS 610
IF LLVDYLNEYAYYA+ AGL YG++L ++GF++SL G+NHKLRILLE ++ KIA F
Sbjct: 481 DIFVWLLVDYLNEYAYYAQAAGLDYGLSLSDNGFELSLAGFNHKLRILLEAVIQKIAKFE 540
Query: 611 VKPDRFLVIKETLLKNYQNFKFQQPYQQAFYYCSLILGDRTWPLMDKLAILSNLGAQDLD 670
VKPDRF VIKET+ K YQN KFQQP++QA YCSL+L D+ WP ++L LS+L A+DL
Sbjct: 541 VKPDRFSVIKETVTKAYQNNKFQQPHEQATNYCSLVLQDQIWPWTEELDALSHLEAEDLA 600
Query: 671 KFVPTLLSSAYLECFIAGNIERTEAESMIDHIEDKFFKGSNPISRPLHPSQYPPNRIVKL 730
FVP LLS ++EC+IAGN+E+ EAESM+ HIED F S PI RPL PSQ+ NR+ +L
Sbjct: 601 NFVPMLLSRTFVECYIAGNVEKDEAESMVKHIEDVLFTDSKPICRPLFPSQFLTNRVTEL 660
Query: 731 ERSIGYFYSAEGLNSNNENSALVHYIQVHRDEFLQNVKLQLFAHVAQQAAFHQLRSVEQL 790
+ +FY EG NS++ENSALVHYIQVH+DEF N KLQLF +A+Q FHQLR++EQL
Sbjct: 661 GTGMKHFYYQEGSNSSDENSALVHYIQVHKDEFSMNSKLQLFELIAKQDTFHQLRTIEQL 720
Query: 791 GYITALAQRNDCGIRGVQFIIQSTVKGPRDIDLRVEAFLEMFEKKLVEMTVDEFQSNVNA 850
GYIT+L+ ND G+ GVQFIIQS+VKGP ID RVE+ L+ E K M+ +EF+SNV
Sbjct: 721 GYITSLSLSNDSGVYGVQFIIQSSVKGPGHIDSRVESLLKDLESKFYNMSDEEFKSNVTN 780
Query: 851 LVDAKLEKFKNLREEARFYWGEISEGTLKFDRRESEVAILKTLTHQDLINFFNEHIKVGA 910
L+D KLEK KNL EE+ FYW EI GTLKF+R ++EVA L+ L + I+FF+E+IKV A
Sbjct: 781 LIDMKLEKDKNLDEESWFYWAEIQTGTLKFNRIDAEVAALRLLKKDEWIDFFDEYIKVDA 840
Query: 911 PRKKSLSVRVYGNLHSKEYSGDLNQPVQPNTVKIDDIFSFRRSQPLYGSFK 962
P KKSLS+ VYGN H KE D ++ + +++I+DI FR+SQPLYGS K
Sbjct: 841 PNKKSLSICVYGNQHLKEMRNDKDK-IPSTSIEIEDIVCFRKSQPLYGSLK 890
BLAST of Cucsa.337080 vs. TAIR10
Match:
AT1G06900.1 (AT1G06900.1 Insulinase (Peptidase family M16) family protein)
HSP 1 Score: 468.4 bits (1204), Expect = 1.1e-131
Identity = 305/945 (32.28%), Postives = 480/945 (50.79%), Query Frame = 1
Query: 32 EVLLVSDPDTDKCAASMTVDVGSFSDPEGLEGLAHFLEHMLFYASEKYPQEDSYSKYITE 91
EV D T K AA+M V +GSF DP +GLAHFLEHMLF S ++P E+ Y Y+++
Sbjct: 95 EVKGKGDHQTKKAAAAMCVSMGSFLDPPEAQGLAHFLEHMLFMGSTEFPDENEYDSYLSK 154
Query: 92 HGGSTNAFTASEETNYYFDVNADCFEEALDRFAQFFIKPLMSPDATMREIKAVDSENQKN 151
HGGS+NA+T E T Y+F+V + + AL RF+QFF+ PLM +A RE+ AVDSE +
Sbjct: 155 HGGSSNAYTEMEHTCYHFEVKREFLQGALKRFSQFFVAPLMKTEAMEREVLAVDSEFNQA 214
Query: 152 LLSDVWRMHQLQCHISSESHPFHKFSTGNWDTLEVQPKAKGLDTRHELLKFYENNYSSNV 211
L +D R+ QLQC+ S++ HPF++F+ GN +L G+D R ++K Y+ Y +
Sbjct: 215 LQNDACRLQQLQCYTSAKGHPFNRFAWGNKKSLS-GAMENGVDLRECIVKLYKEYYHGGL 274
Query: 212 MHLVVYAKENLDKVQILVENIFQDIPNHNCNRANFPGQPCTSEHLQVLVRAIPIKEGHKL 271
M LVV E+LD ++ V +F D+ N + R + + + L R +K+ H L
Sbjct: 275 MKLVVIGGESLDMLESWVVELFGDVKNGSKIRPTLEAEGPIWKGGK-LYRLEAVKDVHIL 334
Query: 272 RIIWPITPEIHHHKEGPCRYLGHLIGHEGEGSLYYVLKTLGWATWLSADESSFTMNLS-- 331
+ W + P + + P YL HL+GHEG GSL+ LK GWAT LSA +N S
Sbjct: 335 DLTWTLPPLRSAYVKKPEDYLAHLLGHEGRGSLHSFLKAKGWATSLSAGVGDDGINRSSL 394
Query: 332 --FFEVVIDLTDVGQEHMQDVIGLLFKYISLLKQSGIWQWIFDELSAICETKFHYADKID 391
F + I LTD G E + D+IG +++Y+ LL+ +WIF EL I F +A++
Sbjct: 395 AYVFGMSIHLTDSGLEKIYDIIGYIYQYLKLLRDVSPQEWIFKELQDIGNMDFRFAEEQP 454
Query: 392 PIDYVVNLSSSMQLYPPEDWLVGSSLPSKFNPKLIGTVLDQLSVDNVRIFWESKKFEGKT 451
DY LS +M YP E + G + ++PKLI ++ + N+RI SK + +
Sbjct: 455 ADDYAAELSENMLAYPVEHVIYGDYVYQTWDPKLIEDLMGFFTPQNMRIDVVSKSIKSEE 514
Query: 452 DKVEKWYGTAYSIEKVSGPLVQDWMQ-SAPDVKLHLPASNIFIPTDLSPKCACEKV---- 511
+ E W+G++Y E V L++ W S D LHLP+ N FIP D S + V
Sbjct: 515 FQQEPWFGSSYIEEDVPLSLMESWSNPSEVDNSLHLPSKNQFIPCDFSIRAINSDVDPKS 574
Query: 512 -KFPVLLKKSSYSSIWYKPDTMFSTPKAYVKIDFICPHADISPEAEVLTAIFTRLLVDYL 571
P + + WYK D F P+A A S + +LT ++ LL D L
Sbjct: 575 QSPPRCIIDEPFMKFWYKLDETFKVPRANTYFRINLKGAYASVKNCLLTELYINLLKDEL 634
Query: 572 NEYAYYAEVAGLYYGINLVESGFQVSLNGYNHKLRILLETIVTKIANFSVKPDRFLVIKE 631
NE Y A +A L +++ ++ + G+N K+ LL I+ +F +RF VIKE
Sbjct: 635 NEIIYQASIAKLETSLSMYGDKLELKVYGFNEKIPALLSKILAIAKSFMPNLERFKVIKE 694
Query: 632 TLLKNYQNFKFQQPYQQAFYYCSLILGDRTWPLMDKLAILSNLGAQDLDKFVPTLLSSAY 691
+ + ++N +P + Y +L R + +KL++L++L DL+ F+P L S +
Sbjct: 695 NMERGFRNTN-MKPLNHSTYLRLQLLCKRIYDSDEKLSVLNDLSLDDLNSFIPELRSQIF 754
Query: 692 LECFIAGNIERTEAESMIDHIEDKFFKGSNPISRPLHPSQ---YPPNRIVKLERSIGYFY 751
+E GN+ EA ++ + +D P S+ H Q +P KL R +
Sbjct: 755 IEALCHGNLSEDEAVNISNIFKDSLTVEPLP-SKCRHGEQITCFPMG--AKLVRDVNV-- 814
Query: 752 SAEGLNSNNENSALVHYIQVHRDEFLQ---NVKLQLFAHVAQQAAFHQLRSVEQLGYITA 811
N + NS + Y Q+ +E L LF + ++ F+QLR+ EQLGY+
Sbjct: 815 ----KNKSETNSVVELYYQIEPEEAQSTRTKAVLDLFHEIIEEPLFNQLRTKEQLGYVVE 874
Query: 812 LAQRNDCGIRGVQFIIQSTVKGPRDIDLRVEAFLEMFEKKLVEMTVDEFQSNVNALVDAK 871
R + G F +QS+ GP + RV+ F++ E L ++ + ++ + ++
Sbjct: 875 CGPRLTYRVHGFCFCVQSSKYGPVHLLGRVDNFIKDIEGLLEQLDDESYEDYRSGMIARL 934
Query: 872 LEKFKNLREEARFYWGEISEGTLKFDRRESEVAILKTLTHQDLINFFNEHIKVGAPRKKS 931
LEK +L E W +I + FD E L+++ +D+I+++ + + +P+ +
Sbjct: 935 LEKDPSLLSETNDLWSQIVDKRYMFDFSHKEAEELRSIQKKDVISWYKTYFRESSPKCRR 994
Query: 932 LSVRVYG-NLHSKEYSGDLNQPVQPNTVKIDDIFSFRRSQPLYGS 960
L+VRV+G + + KE D Q I D +F+ + Y S
Sbjct: 995 LAVRVWGCDTNMKETQTD-----QKAVQVIADAVAFKSTSKFYPS 1022
HSP 2 Score: 33.5 bits (75), Expect = 8.9e-01
Identity = 15/29 (51.72%), Postives = 21/29 (72.41%), Query Frame = 1
Query: 12 IVKARNDKREYKRIVLKNSLEVLLVSDPD 41
+VK+ ND+R Y+ I L+N L LL+ DPD
Sbjct: 14 VVKSPNDRRLYRVIELENGLCALLIHDPD 42
BLAST of Cucsa.337080 vs. TAIR10
Match:
AT3G57460.1 (AT3G57460.1 catalytics;metal ion binding)
HSP 1 Score: 156.8 bits (395), Expect = 6.9e-38
Identity = 89/207 (43.00%), Postives = 112/207 (54.11%), Query Frame = 1
Query: 256 LQVLVRAIPIKEGHKLRIIWPITPEIHHHKEGPCRYLGHLIGHEGEGSLYYVLKTLGWAT 315
L+VLV+A+PIK+GH L + WP+TP IHH++E R WAT
Sbjct: 35 LEVLVKAVPIKQGHNLTVSWPVTPSIHHYEEAHAR----------------------WAT 94
Query: 316 WLSADESSFTMNLSFFEVVIDLTDVGQEHMQDVIGLLFKYISLLKQSGIWQWIFDELSAI 375
L A E +T+ SFF V I+LTD EHM+D++GLLF+ I LL+QSG+ QWIFDELSAI
Sbjct: 95 GLYAGEPDWTVEYSFFNVSINLTDACHEHMKDILGLLFRQIKLLQQSGVSQWIFDELSAI 154
Query: 376 CETKFHYADKIDPIDYVVNLSSSMQLYPPEDWLVGSSLPSKFNPKLIGTVLDQLSVDNVR 435
E +FHY KIDPI Y VN+SS+M L+G
Sbjct: 155 FEAEFHYQAKIDPISYAVNISSNM-------------------TNLLGIK---------- 183
Query: 436 IFWESKKFEGKTDKVEKWYGTAYSIEK 463
+EG+TDKVE WY TA I K
Sbjct: 215 -------YEGQTDKVEPWYNTAEKIIK 183
HSP 2 Score: 118.2 bits (295), Expect = 2.7e-26
Identity = 59/109 (54.13%), Postives = 79/109 (72.48%), Query Frame = 1
Query: 517 WYKPDTMFSTPKAYVKIDFICPHADISPEAEVLTAIFTRLLVDYLNEYAYYAEVAGLYYG 576
WY +T K +++DF CP A SP VL+ LLVDYLNEYAYYA+ A L+YG
Sbjct: 174 WY--NTAEKIIKFTIQMDFNCPLAVSSPATVVLSNSSVWLLVDYLNEYAYYAQAARLHYG 233
Query: 577 INLVESGFQVSLNGYNHKLRILLETIVTKIANFSVKPDRFLVIKETLLK 626
++L ++GF++SL G+NHKLRILLE ++ K+ANF VKPDRF V+K T+L+
Sbjct: 234 LSLSDNGFELSLTGFNHKLRILLEAVIQKMANFQVKPDRFSVVKVTVLR 280
HSP 3 Score: 85.1 bits (209), Expect = 2.6e-16
Identity = 50/125 (40.00%), Postives = 75/125 (60.00%), Query Frame = 1
Query: 838 EMTVDEFQSNVNALVDAKLEKFKNLREEARFYWGEISEGTLKFDRRES-EVAILKTLTHQ 897
E+++ F + L++A ++K N + +K DR +V +L+ L +
Sbjct: 240 ELSLTGFNHKLRILLEAVIQKMANFQ--------------VKPDRFSVVKVTVLRELKKE 299
Query: 898 DLINFFNEHIKVGAPRKKSLSVRVYGNLHSKEYSGDLNQPVQPNTVKIDDIFSFRRSQPL 957
+LINFF+E+ KVGAP++KSLSV VYGN H KE S D ++ V +++I+DI FR SQPL
Sbjct: 300 ELINFFDEYTKVGAPKRKSLSVCVYGNQHLKEMSSDKDKVVS-TSIEIEDIVGFRNSQPL 349
Query: 958 YGSFK 962
Y S K
Sbjct: 360 YASLK 349
HSP 4 Score: 43.9 bits (102), Expect = 6.6e-04
Identity = 21/28 (75.00%), Postives = 25/28 (89.29%), Query Frame = 1
Query: 12 IVKARNDKREYKRIVLKNSLEVLLVSDP 40
I+KAR DKREY+RIVLKNSLEVL+ + P
Sbjct: 16 ILKARTDKREYRRIVLKNSLEVLVKAVP 43
BLAST of Cucsa.337080 vs. TAIR10
Match:
AT3G02090.2 (AT3G02090.2 Insulinase (Peptidase family M16) protein)
HSP 1 Score: 56.6 bits (135), Expect = 9.8e-08
Identity = 41/154 (26.62%), Postives = 67/154 (43.51%), Query Frame = 1
Query: 10 HEIVKARN---DKREYKRIVLKNSLEVLLVSDPDTDKCAASMTVDVGSFSDPEGLEGLAH 69
H I+ + N E + L N L V S+ + +D GS + + G AH
Sbjct: 82 HPILASHNHILSAPETRVTTLPNGLRVATESNLSAKTATVGVWIDAGSRFESDETNGTAH 141
Query: 70 FLEHMLFYASEKYPQEDSYSKYITEHGGSTNAFTASEETNYYFDVNADCFEEALDRFAQF 129
FLEHM+F +++ + + I + GG NA+T+ E+T YY V +ALD A
Sbjct: 142 FLEHMIFKGTDRRTVR-ALEEEIEDIGGHLNAYTSREQTTYYAKVLDSNVNQALDVLADI 201
Query: 130 FIKPLMSP-------DATMREIKAVDSENQKNLL 154
D +RE++ V+ + + +L
Sbjct: 202 LQNSKFEEQRINRERDVILREMQEVEGQTDEVVL 234
BLAST of Cucsa.337080 vs. NCBI nr
Match:
gi|778684988|ref|XP_011652139.1| (PREDICTED: zinc-metallopeptidase, peroxisomal-like isoform X2 [Cucumis sativus])
HSP 1 Score: 1950.3 bits (5051), Expect = 0.0e+00
Identity = 960/967 (99.28%), Postives = 961/967 (99.38%), Query Frame = 1
Query: 1 MAVGKEQMTHEIVKARNDKREYKRIVLKNSLEVLLVSDPDTDKCAASMTVDVGSFSDPEG 60
MAVGKEQMTHEIVKARNDKREYKRIVLKNSLEVLLVSDPDTDKCAASMTVDVGSFSDPEG
Sbjct: 1 MAVGKEQMTHEIVKARNDKREYKRIVLKNSLEVLLVSDPDTDKCAASMTVDVGSFSDPEG 60
Query: 61 LEGLAHFLEHMLFYASEKYPQEDSYSKYITEHGGSTNAFTASEETNYYFDVNADCFEEAL 120
LEGLAHFLEHMLFYASEKYP EDSYSKYI EHGGSTNAFTASEETNYYFDVNADCFEEAL
Sbjct: 61 LEGLAHFLEHMLFYASEKYPLEDSYSKYIKEHGGSTNAFTASEETNYYFDVNADCFEEAL 120
Query: 121 DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDVWRMHQLQCHISSESHPFHKFSTGN 180
DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDVWRMHQLQCHISSESHPFHKFSTGN
Sbjct: 121 DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDVWRMHQLQCHISSESHPFHKFSTGN 180
Query: 181 WDTLEVQPKAKGLDTRHELLKFYENNYSSNVMHLVVYAKENLDKVQILVENIFQDIPNHN 240
WDTLEVQPKAKGLDTRHELLKFYEN+YSSNVMHLVVYAKENLDKVQILVENIFQDIPNHN
Sbjct: 181 WDTLEVQPKAKGLDTRHELLKFYENSYSSNVMHLVVYAKENLDKVQILVENIFQDIPNHN 240
Query: 241 CNRANFPGQPCTSEHLQVLVRAIPIKEGHKLRIIWPITPEIHHHKEGPCRYLGHLIGHEG 300
CNRANFPGQPC SEHLQVLVRAIPIKEGHKLRIIWPITPEIHHHKEGPCRYLGHLIGHEG
Sbjct: 241 CNRANFPGQPCPSEHLQVLVRAIPIKEGHKLRIIWPITPEIHHHKEGPCRYLGHLIGHEG 300
Query: 301 EGSLYYVLKTLGWATWLSADESSFTMNLSFFEVVIDLTDVGQEHMQDVIGLLFKYISLLK 360
EGSLYYVLKTLGWATWLSADESSFTMNLSFFEVVIDLTDVGQEHMQDVIGLLFKYISLLK
Sbjct: 301 EGSLYYVLKTLGWATWLSADESSFTMNLSFFEVVIDLTDVGQEHMQDVIGLLFKYISLLK 360
Query: 361 QSGIWQWIFDELSAICETKFHYADKIDPIDYVVNLSSSMQLYPPEDWLVGSSLPSKFNPK 420
QSGIWQWIFDELSAICETKFHYADKIDPIDYVVNLSSSMQLYPPEDWLVGSSLPSKFNPK
Sbjct: 361 QSGIWQWIFDELSAICETKFHYADKIDPIDYVVNLSSSMQLYPPEDWLVGSSLPSKFNPK 420
Query: 421 LIGTVLDQLSVDNVRIFWESKKFEGKTDKVEKWYGTAYSIEKVSGPLVQDWMQSAPDVKL 480
LIGTVLDQLSVDNVRIFWESKKFEGKTDKVEKWYGTAYSIEKVSGPLVQDWMQSAPDV L
Sbjct: 421 LIGTVLDQLSVDNVRIFWESKKFEGKTDKVEKWYGTAYSIEKVSGPLVQDWMQSAPDVNL 480
Query: 481 HLPASNIFIPTDLSPKCACEKVKFPVLLKKSSYSSIWYKPDTMFSTPKAYVKIDFICPHA 540
HLPASNIFIPTDLSPKCACEKVKFPVLLKKSSYSSIWYKPDTMFSTPKAYVKIDFICPHA
Sbjct: 481 HLPASNIFIPTDLSPKCACEKVKFPVLLKKSSYSSIWYKPDTMFSTPKAYVKIDFICPHA 540
Query: 541 DISPEAEVLTAIFTRLLVDYLNEYAYYAEVAGLYYGINLVESGFQVSLNGYNHKLRILLE 600
DISPEAEVLTAIFTRLLVDYLNEYAYYAEVAGLYYGINLVESGFQVSLNGYNHKLRILLE
Sbjct: 541 DISPEAEVLTAIFTRLLVDYLNEYAYYAEVAGLYYGINLVESGFQVSLNGYNHKLRILLE 600
Query: 601 TIVTKIANFSVKPDRFLVIKETLLKNYQNFKFQQPYQQAFYYCSLILGDRTWPLMDKLAI 660
TIVTKIANFSVKPDRFLVIKET LKNYQNFKFQQPYQQAFYYCSLILGDRTWPLMDKLAI
Sbjct: 601 TIVTKIANFSVKPDRFLVIKETFLKNYQNFKFQQPYQQAFYYCSLILGDRTWPLMDKLAI 660
Query: 661 LSNLGAQDLDKFVPTLLSSAYLECFIAGNIERTEAESMIDHIEDKFFKGSNPISRPLHPS 720
LSNLGAQDLDKFVPTLLSSA LECFIAGNIERTEAESMIDHIEDKFFKGSNPISRPLHPS
Sbjct: 661 LSNLGAQDLDKFVPTLLSSANLECFIAGNIERTEAESMIDHIEDKFFKGSNPISRPLHPS 720
Query: 721 QYPPNRIVKLERSIGYFYSAEGLNSNNENSALVHYIQVHRDEFLQNVKLQLFAHVAQQAA 780
QYPPNRIVKLERSIGYFYSAEGLNSNNENSALVHYIQVHRDEFLQNVKLQLFAHVAQQAA
Sbjct: 721 QYPPNRIVKLERSIGYFYSAEGLNSNNENSALVHYIQVHRDEFLQNVKLQLFAHVAQQAA 780
Query: 781 FHQLRSVEQLGYITALAQRNDCGIRGVQFIIQSTVKGPRDIDLRVEAFLEMFEKKLVEMT 840
FHQLRSVEQLGYITALAQRNDCGIRGVQFIIQSTVKGPRDIDLRVEAFLEMFEKKLVEMT
Sbjct: 781 FHQLRSVEQLGYITALAQRNDCGIRGVQFIIQSTVKGPRDIDLRVEAFLEMFEKKLVEMT 840
Query: 841 VDEFQSNVNALVDAKLEKFKNLREEARFYWGEISEGTLKFDRRESEVAILKTLTHQDLIN 900
VDEFQSNVNALVDAKLEKFKNLREEARFYWGEISEGTLKFDRRESEVAILKTLTHQDLIN
Sbjct: 841 VDEFQSNVNALVDAKLEKFKNLREEARFYWGEISEGTLKFDRRESEVAILKTLTHQDLIN 900
Query: 901 FFNEHIKVGAPRKKSLSVRVYGNLHSKEYSGDLNQPVQPNTVKIDDIFSFRRSQPLYGSF 960
FFNEHIKVGAPRKKSLSVRVYGNLHSKEYSGDLNQPVQPNTVKIDDIFSFRRSQPLYGSF
Sbjct: 901 FFNEHIKVGAPRKKSLSVRVYGNLHSKEYSGDLNQPVQPNTVKIDDIFSFRRSQPLYGSF 960
Query: 961 KYGNVKL 968
KYGNVKL
Sbjct: 961 KYGNVKL 967
BLAST of Cucsa.337080 vs. NCBI nr
Match:
gi|659084909|ref|XP_008443138.1| (PREDICTED: zinc-metallopeptidase, peroxisomal-like isoform X1 [Cucumis melo])
HSP 1 Score: 1818.1 bits (4708), Expect = 0.0e+00
Identity = 896/969 (92.47%), Postives = 932/969 (96.18%), Query Frame = 1
Query: 1 MAVGKEQMTHEIVKARNDKREYKRIVLKNSLEVLLVSDPDTDKCAASMTVDVGSFSDPEG 60
MAVGKEQ+THEIVK RNDKREY+RIVLKNSLEVLLVSDPDTDK AASMTVDVGSFSDPEG
Sbjct: 1 MAVGKEQVTHEIVKPRNDKREYRRIVLKNSLEVLLVSDPDTDKSAASMTVDVGSFSDPEG 60
Query: 61 LEGLAHFLEHMLFYASEKYPQEDSYSKYITEHGGSTNAFTASEETNYYFDVNADCFEEAL 120
LEGLAHFLEHMLFYASEKYPQEDSYSKYITEHGGS NAFTASEETNYYFDVNADCFEEAL
Sbjct: 61 LEGLAHFLEHMLFYASEKYPQEDSYSKYITEHGGSMNAFTASEETNYYFDVNADCFEEAL 120
Query: 121 DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDVWRMHQLQCHISSESHPFHKFSTGN 180
DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSD WRMHQLQ HISSESHPFHKFSTGN
Sbjct: 121 DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQSHISSESHPFHKFSTGN 180
Query: 181 WDTLEVQPKAKGLDTRHELLKFYENNYSSNVMHLVVYAKENLDKVQILVENIFQDIPNHN 240
W+TLEVQPKAKGLDTRHELLKFYENNYSSNVMHLVVYAKE LDKVQILVENIFQDIPNHN
Sbjct: 181 WETLEVQPKAKGLDTRHELLKFYENNYSSNVMHLVVYAKEKLDKVQILVENIFQDIPNHN 240
Query: 241 CNRANFPGQPCTSEHLQVLVRAIPIKEGHKLRIIWPITPEIHHHKEGPCRYLGHLIGHEG 300
CNRANFPGQPCTSEHLQVLVRAIPIKEGHKLRIIWPITPEIHH+KEGPCRYLGHLIGHEG
Sbjct: 241 CNRANFPGQPCTSEHLQVLVRAIPIKEGHKLRIIWPITPEIHHYKEGPCRYLGHLIGHEG 300
Query: 301 EGSLYYVLKTLGWATWLSADESSFTMNLSFFEVVIDLTDVGQEHMQDVIGLLFKYISLLK 360
EGSLYYVLKTLGWAT LSA ES TMN SFF+VVIDLTDVGQEHMQ+VIGLLF+YISLLK
Sbjct: 301 EGSLYYVLKTLGWATGLSAGESDSTMNFSFFKVVIDLTDVGQEHMQEVIGLLFRYISLLK 360
Query: 361 QSGIWQWIFDELSAICETKFHYADKIDPIDYVVNLSSSMQLYPPEDWLVGSSLPSKFNPK 420
QSGI QWIFDELSAICETKFHYADKI PI+YV++LS +MQLYPPEDWLVGSSLPSKF+PK
Sbjct: 361 QSGICQWIFDELSAICETKFHYADKIRPINYVISLSLNMQLYPPEDWLVGSSLPSKFDPK 420
Query: 421 LIGTVLDQLSVDNVRIFWESKKFEGKTDKVEKWYGTAYSIEKVSGPLVQDWMQSAPDVKL 480
LIGTVLDQLSVDNVRIFWESKKFEGKTDKVEKWYGTAYSIEK+SG LVQDWMQSAPDV L
Sbjct: 421 LIGTVLDQLSVDNVRIFWESKKFEGKTDKVEKWYGTAYSIEKISGHLVQDWMQSAPDVNL 480
Query: 481 HLPASNIFIPTDLSPKCACEKVKFPVLLKKSSYSSIWYKPDTMFSTPKAYVKIDFICPHA 540
HLPASNIFIPTDLS K ACEKV+FPVLL+KSSYSSIWYKPDTMFSTPKAYVKIDFICPHA
Sbjct: 481 HLPASNIFIPTDLSLKRACEKVEFPVLLRKSSYSSIWYKPDTMFSTPKAYVKIDFICPHA 540
Query: 541 DISPEAEVLTAIFTRLLVDYLNEYAYYAEVAGLYYGINLVESGFQVSLNGYNHKLRILLE 600
DISPEAEVLTAIFTRLL+DYLNEYAYYAEVAGLYYGINL++SGFQV+LNGYNHKLR+LLE
Sbjct: 541 DISPEAEVLTAIFTRLLMDYLNEYAYYAEVAGLYYGINLIDSGFQVTLNGYNHKLRVLLE 600
Query: 601 TIVTKIANFSVKPDRFLVIKETLLKNYQNFKFQQPYQQAFYYCSLILGDRTWPLMDKLAI 660
TIV KIA FSVKPDR+LVIKETLLK+YQNFKFQQPYQQA YYCSLIL DRTWPLMD+LAI
Sbjct: 601 TIVEKIAKFSVKPDRYLVIKETLLKDYQNFKFQQPYQQALYYCSLILEDRTWPLMDELAI 660
Query: 661 LSNLGAQDLDKFVPTLLSSAYLECFIAGNIERTEAESMIDHIEDKFFKGSNPISRPLHPS 720
L +LGA+DLDKFVPTLLSSAYLECFIAGNIERTEAESMIDHIED FFKG NPISRPL+PS
Sbjct: 661 LPHLGARDLDKFVPTLLSSAYLECFIAGNIERTEAESMIDHIEDTFFKGPNPISRPLNPS 720
Query: 721 QYPPNRIVKLERSIGYFYSAEGLNSNNENSALVHYIQVHRDEFLQNVKLQLFAHVAQQAA 780
QYP NRIVKLER IGY YSAEGLNSN+ENSALVHYIQVHRDEFLQNVKLQLFAHVA+QAA
Sbjct: 721 QYPANRIVKLERGIGYLYSAEGLNSNDENSALVHYIQVHRDEFLQNVKLQLFAHVAKQAA 780
Query: 781 FHQLRSVEQLGYITALAQRNDCGIRGVQFIIQSTVKGPRDIDLRVEAFLEMFEKKLVEMT 840
FHQLR+VEQLGYIT LAQ N CGIRGVQFIIQSTVKGPR IDLRVEAFL+MFEKKLVEMT
Sbjct: 781 FHQLRTVEQLGYITVLAQSNHCGIRGVQFIIQSTVKGPRGIDLRVEAFLDMFEKKLVEMT 840
Query: 841 VDEFQSNVNALVDAKLEKFKNLREEARFYWGEISEGTLKFDRRESEVAILKTLTHQDLIN 900
+DEF+SNVNALVDAKLEKFKNL+EE+RFYWGEIS GTLKFDRRESEVA+LKTLTHQDLIN
Sbjct: 841 IDEFKSNVNALVDAKLEKFKNLKEESRFYWGEISGGTLKFDRRESEVALLKTLTHQDLIN 900
Query: 901 FFNEHIKVGAPRKKSLSVRVYGNLHSKEYSGDLNQPVQPNTVKIDDIFSFRRSQPLYGSF 960
FFNEHIKVGAP KKSLSVRVYGNLHS EYSGDLNQP+QPNTVKIDDIFSFRRSQPLYGSF
Sbjct: 901 FFNEHIKVGAPCKKSLSVRVYGNLHSSEYSGDLNQPLQPNTVKIDDIFSFRRSQPLYGSF 960
Query: 961 K--YGNVKL 968
K YGNVKL
Sbjct: 961 KGAYGNVKL 969
BLAST of Cucsa.337080 vs. NCBI nr
Match:
gi|659084913|ref|XP_008443140.1| (PREDICTED: zinc-metallopeptidase, peroxisomal-like isoform X2 [Cucumis melo])
HSP 1 Score: 1692.9 bits (4383), Expect = 0.0e+00
Identity = 831/903 (92.03%), Postives = 867/903 (96.01%), Query Frame = 1
Query: 67 FLEHMLFYASEKYPQEDSYSKYITEHGGSTNAFTASEETNYYFDVNADCFEEALDRFAQF 126
+ +HMLFYASEKYPQEDSYSKYITEHGGS NAFTASEETNYYFDVNADCFEEALDRFAQF
Sbjct: 3 YSKHMLFYASEKYPQEDSYSKYITEHGGSMNAFTASEETNYYFDVNADCFEEALDRFAQF 62
Query: 127 FIKPLMSPDATMREIKAVDSENQKNLLSDVWRMHQLQCHISSESHPFHKFSTGNWDTLEV 186
FIKPLMSPDATMREIKAVDSENQKNLLSD WRMHQLQ HISSESHPFHKFSTGNW+TLEV
Sbjct: 63 FIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQSHISSESHPFHKFSTGNWETLEV 122
Query: 187 QPKAKGLDTRHELLKFYENNYSSNVMHLVVYAKENLDKVQILVENIFQDIPNHNCNRANF 246
QPKAKGLDTRHELLKFYENNYSSNVMHLVVYAKE LDKVQILVENIFQDIPNHNCNRANF
Sbjct: 123 QPKAKGLDTRHELLKFYENNYSSNVMHLVVYAKEKLDKVQILVENIFQDIPNHNCNRANF 182
Query: 247 PGQPCTSEHLQVLVRAIPIKEGHKLRIIWPITPEIHHHKEGPCRYLGHLIGHEGEGSLYY 306
PGQPCTSEHLQVLVRAIPIKEGHKLRIIWPITPEIHH+KEGPCRYLGHLIGHEGEGSLYY
Sbjct: 183 PGQPCTSEHLQVLVRAIPIKEGHKLRIIWPITPEIHHYKEGPCRYLGHLIGHEGEGSLYY 242
Query: 307 VLKTLGWATWLSADESSFTMNLSFFEVVIDLTDVGQEHMQDVIGLLFKYISLLKQSGIWQ 366
VLKTLGWAT LSA ES TMN SFF+VVIDLTDVGQEHMQ+VIGLLF+YISLLKQSGI Q
Sbjct: 243 VLKTLGWATGLSAGESDSTMNFSFFKVVIDLTDVGQEHMQEVIGLLFRYISLLKQSGICQ 302
Query: 367 WIFDELSAICETKFHYADKIDPIDYVVNLSSSMQLYPPEDWLVGSSLPSKFNPKLIGTVL 426
WIFDELSAICETKFHYADKI PI+YV++LS +MQLYPPEDWLVGSSLPSKF+PKLIGTVL
Sbjct: 303 WIFDELSAICETKFHYADKIRPINYVISLSLNMQLYPPEDWLVGSSLPSKFDPKLIGTVL 362
Query: 427 DQLSVDNVRIFWESKKFEGKTDKVEKWYGTAYSIEKVSGPLVQDWMQSAPDVKLHLPASN 486
DQLSVDNVRIFWESKKFEGKTDKVEKWYGTAYSIEK+SG LVQDWMQSAPDV LHLPASN
Sbjct: 363 DQLSVDNVRIFWESKKFEGKTDKVEKWYGTAYSIEKISGHLVQDWMQSAPDVNLHLPASN 422
Query: 487 IFIPTDLSPKCACEKVKFPVLLKKSSYSSIWYKPDTMFSTPKAYVKIDFICPHADISPEA 546
IFIPTDLS K ACEKV+FPVLL+KSSYSSIWYKPDTMFSTPKAYVKIDFICPHADISPEA
Sbjct: 423 IFIPTDLSLKRACEKVEFPVLLRKSSYSSIWYKPDTMFSTPKAYVKIDFICPHADISPEA 482
Query: 547 EVLTAIFTRLLVDYLNEYAYYAEVAGLYYGINLVESGFQVSLNGYNHKLRILLETIVTKI 606
EVLTAIFTRLL+DYLNEYAYYAEVAGLYYGINL++SGFQV+LNGYNHKLR+LLETIV KI
Sbjct: 483 EVLTAIFTRLLMDYLNEYAYYAEVAGLYYGINLIDSGFQVTLNGYNHKLRVLLETIVEKI 542
Query: 607 ANFSVKPDRFLVIKETLLKNYQNFKFQQPYQQAFYYCSLILGDRTWPLMDKLAILSNLGA 666
A FSVKPDR+LVIKETLLK+YQNFKFQQPYQQA YYCSLIL DRTWPLMD+LAIL +LGA
Sbjct: 543 AKFSVKPDRYLVIKETLLKDYQNFKFQQPYQQALYYCSLILEDRTWPLMDELAILPHLGA 602
Query: 667 QDLDKFVPTLLSSAYLECFIAGNIERTEAESMIDHIEDKFFKGSNPISRPLHPSQYPPNR 726
+DLDKFVPTLLSSAYLECFIAGNIERTEAESMIDHIED FFKG NPISRPL+PSQYP NR
Sbjct: 603 RDLDKFVPTLLSSAYLECFIAGNIERTEAESMIDHIEDTFFKGPNPISRPLNPSQYPANR 662
Query: 727 IVKLERSIGYFYSAEGLNSNNENSALVHYIQVHRDEFLQNVKLQLFAHVAQQAAFHQLRS 786
IVKLER IGY YSAEGLNSN+ENSALVHYIQVHRDEFLQNVKLQLFAHVA+QAAFHQLR+
Sbjct: 663 IVKLERGIGYLYSAEGLNSNDENSALVHYIQVHRDEFLQNVKLQLFAHVAKQAAFHQLRT 722
Query: 787 VEQLGYITALAQRNDCGIRGVQFIIQSTVKGPRDIDLRVEAFLEMFEKKLVEMTVDEFQS 846
VEQLGYIT LAQ N CGIRGVQFIIQSTVKGPR IDLRVEAFL+MFEKKLVEMT+DEF+S
Sbjct: 723 VEQLGYITVLAQSNHCGIRGVQFIIQSTVKGPRGIDLRVEAFLDMFEKKLVEMTIDEFKS 782
Query: 847 NVNALVDAKLEKFKNLREEARFYWGEISEGTLKFDRRESEVAILKTLTHQDLINFFNEHI 906
NVNALVDAKLEKFKNL+EE+RFYWGEIS GTLKFDRRESEVA+LKTLTHQDLINFFNEHI
Sbjct: 783 NVNALVDAKLEKFKNLKEESRFYWGEISGGTLKFDRRESEVALLKTLTHQDLINFFNEHI 842
Query: 907 KVGAPRKKSLSVRVYGNLHSKEYSGDLNQPVQPNTVKIDDIFSFRRSQPLYGSFK--YGN 966
KVGAP KKSLSVRVYGNLHS EYSGDLNQP+QPNTVKIDDIFSFRRSQPLYGSFK YGN
Sbjct: 843 KVGAPCKKSLSVRVYGNLHSSEYSGDLNQPLQPNTVKIDDIFSFRRSQPLYGSFKGAYGN 902
Query: 967 VKL 968
VKL
Sbjct: 903 VKL 905
BLAST of Cucsa.337080 vs. NCBI nr
Match:
gi|778684984|ref|XP_011652138.1| (PREDICTED: zinc-metallopeptidase, peroxisomal-like isoform X1 [Cucumis sativus])
HSP 1 Score: 1663.7 bits (4307), Expect = 0.0e+00
Identity = 816/821 (99.39%), Postives = 817/821 (99.51%), Query Frame = 1
Query: 147 ENQKNLLSDVWRMHQLQCHISSESHPFHKFSTGNWDTLEVQPKAKGLDTRHELLKFYENN 206
ENQKNLLSDVWRMHQLQCHISSESHPFHKFSTGNWDTLEVQPKAKGLDTRHELLKFYEN+
Sbjct: 6 ENQKNLLSDVWRMHQLQCHISSESHPFHKFSTGNWDTLEVQPKAKGLDTRHELLKFYENS 65
Query: 207 YSSNVMHLVVYAKENLDKVQILVENIFQDIPNHNCNRANFPGQPCTSEHLQVLVRAIPIK 266
YSSNVMHLVVYAKENLDKVQILVENIFQDIPNHNCNRANFPGQPC SEHLQVLVRAIPIK
Sbjct: 66 YSSNVMHLVVYAKENLDKVQILVENIFQDIPNHNCNRANFPGQPCPSEHLQVLVRAIPIK 125
Query: 267 EGHKLRIIWPITPEIHHHKEGPCRYLGHLIGHEGEGSLYYVLKTLGWATWLSADESSFTM 326
EGHKLRIIWPITPEIHHHKEGPCRYLGHLIGHEGEGSLYYVLKTLGWATWLSADESSFTM
Sbjct: 126 EGHKLRIIWPITPEIHHHKEGPCRYLGHLIGHEGEGSLYYVLKTLGWATWLSADESSFTM 185
Query: 327 NLSFFEVVIDLTDVGQEHMQDVIGLLFKYISLLKQSGIWQWIFDELSAICETKFHYADKI 386
NLSFFEVVIDLTDVGQEHMQDVIGLLFKYISLLKQSGIWQWIFDELSAICETKFHYADKI
Sbjct: 186 NLSFFEVVIDLTDVGQEHMQDVIGLLFKYISLLKQSGIWQWIFDELSAICETKFHYADKI 245
Query: 387 DPIDYVVNLSSSMQLYPPEDWLVGSSLPSKFNPKLIGTVLDQLSVDNVRIFWESKKFEGK 446
DPIDYVVNLSSSMQLYPPEDWLVGSSLPSKFNPKLIGTVLDQLSVDNVRIFWESKKFEGK
Sbjct: 246 DPIDYVVNLSSSMQLYPPEDWLVGSSLPSKFNPKLIGTVLDQLSVDNVRIFWESKKFEGK 305
Query: 447 TDKVEKWYGTAYSIEKVSGPLVQDWMQSAPDVKLHLPASNIFIPTDLSPKCACEKVKFPV 506
TDKVEKWYGTAYSIEKVSGPLVQDWMQSAPDV LHLPASNIFIPTDLSPKCACEKVKFPV
Sbjct: 306 TDKVEKWYGTAYSIEKVSGPLVQDWMQSAPDVNLHLPASNIFIPTDLSPKCACEKVKFPV 365
Query: 507 LLKKSSYSSIWYKPDTMFSTPKAYVKIDFICPHADISPEAEVLTAIFTRLLVDYLNEYAY 566
LLKKSSYSSIWYKPDTMFSTPKAYVKIDFICPHADISPEAEVLTAIFTRLLVDYLNEYAY
Sbjct: 366 LLKKSSYSSIWYKPDTMFSTPKAYVKIDFICPHADISPEAEVLTAIFTRLLVDYLNEYAY 425
Query: 567 YAEVAGLYYGINLVESGFQVSLNGYNHKLRILLETIVTKIANFSVKPDRFLVIKETLLKN 626
YAEVAGLYYGINLVESGFQVSLNGYNHKLRILLETIVTKIANFSVKPDRFLVIKET LKN
Sbjct: 426 YAEVAGLYYGINLVESGFQVSLNGYNHKLRILLETIVTKIANFSVKPDRFLVIKETFLKN 485
Query: 627 YQNFKFQQPYQQAFYYCSLILGDRTWPLMDKLAILSNLGAQDLDKFVPTLLSSAYLECFI 686
YQNFKFQQPYQQAFYYCSLILGDRTWPLMDKLAILSNLGAQDLDKFVPTLLSSA LECFI
Sbjct: 486 YQNFKFQQPYQQAFYYCSLILGDRTWPLMDKLAILSNLGAQDLDKFVPTLLSSANLECFI 545
Query: 687 AGNIERTEAESMIDHIEDKFFKGSNPISRPLHPSQYPPNRIVKLERSIGYFYSAEGLNSN 746
AGNIERTEAESMIDHIEDKFFKGSNPISRPLHPSQYPPNRIVKLERSIGYFYSAEGLNSN
Sbjct: 546 AGNIERTEAESMIDHIEDKFFKGSNPISRPLHPSQYPPNRIVKLERSIGYFYSAEGLNSN 605
Query: 747 NENSALVHYIQVHRDEFLQNVKLQLFAHVAQQAAFHQLRSVEQLGYITALAQRNDCGIRG 806
NENSALVHYIQVHRDEFLQNVKLQLFAHVAQQAAFHQLRSVEQLGYITALAQRNDCGIRG
Sbjct: 606 NENSALVHYIQVHRDEFLQNVKLQLFAHVAQQAAFHQLRSVEQLGYITALAQRNDCGIRG 665
Query: 807 VQFIIQSTVKGPRDIDLRVEAFLEMFEKKLVEMTVDEFQSNVNALVDAKLEKFKNLREEA 866
VQFIIQSTVKGPRDIDLRVEAFLEMFEKKLVEMTVDEFQSNVNALVDAKLEKFKNLREEA
Sbjct: 666 VQFIIQSTVKGPRDIDLRVEAFLEMFEKKLVEMTVDEFQSNVNALVDAKLEKFKNLREEA 725
Query: 867 RFYWGEISEGTLKFDRRESEVAILKTLTHQDLINFFNEHIKVGAPRKKSLSVRVYGNLHS 926
RFYWGEISEGTLKFDRRESEVAILKTLTHQDLINFFNEHIKVGAPRKKSLSVRVYGNLHS
Sbjct: 726 RFYWGEISEGTLKFDRRESEVAILKTLTHQDLINFFNEHIKVGAPRKKSLSVRVYGNLHS 785
Query: 927 KEYSGDLNQPVQPNTVKIDDIFSFRRSQPLYGSFKYGNVKL 968
KEYSGDLNQPVQPNTVKIDDIFSFRRSQPLYGSFKYGNVKL
Sbjct: 786 KEYSGDLNQPVQPNTVKIDDIFSFRRSQPLYGSFKYGNVKL 826
BLAST of Cucsa.337080 vs. NCBI nr
Match:
gi|778684991|ref|XP_011652140.1| (PREDICTED: LOW QUALITY PROTEIN: zinc-metallopeptidase, peroxisomal [Cucumis sativus])
HSP 1 Score: 1629.8 bits (4219), Expect = 0.0e+00
Identity = 811/887 (91.43%), Postives = 837/887 (94.36%), Query Frame = 1
Query: 1 MAVGKEQMTHEIVKARNDKREYKRIVLKNSLEVLLVSDPDTDKCAASMTVDVGSFSDPEG 60
M VGKEQ+THEIVK RNDKREY+RIVLKNSLEVLLVSDPDTDKCAASMTVDVGSFSDPEG
Sbjct: 1 MEVGKEQVTHEIVKPRNDKREYRRIVLKNSLEVLLVSDPDTDKCAASMTVDVGSFSDPEG 60
Query: 61 LEGLAHFLEHMLFYASEKYPQEDSYSKYITEHGGSTNAFTASEETNYYFDVNADCFEEAL 120
LEGLAHFLEHMLFYASEKYP EDSYSKYI EHGGSTNAFTASE TNYYFDVNADCFEEAL
Sbjct: 61 LEGLAHFLEHMLFYASEKYPLEDSYSKYIKEHGGSTNAFTASEGTNYYFDVNADCFEEAL 120
Query: 121 DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDVWRMHQLQCHISSESHPFHKFSTGN 180
DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDVWRMHQLQ HISSESHPFHKFSTGN
Sbjct: 121 DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDVWRMHQLQRHISSESHPFHKFSTGN 180
Query: 181 WDTLEVQPKAKGLDTRHELLKFYENNYSSNVMHLVVYAKENLDKVQILVENIFQDIPNHN 240
WDTLEV+PKAKGLDTRHELLKFYEN+YSSNVMHLVVYAKE LD+VQILVENIFQDIPNHN
Sbjct: 181 WDTLEVRPKAKGLDTRHELLKFYENSYSSNVMHLVVYAKEKLDEVQILVENIFQDIPNHN 240
Query: 241 CNRANFPGQPCTSEHLQVLVRAIPIKEGHKLRIIWPITPEIHHHKEGPCRYLGHLIGHEG 300
CNRANFPGQPCTSEHLQVLVRAIPIKEGHKLRIIWPITP IHH+KEGPCRYL HLIGHEG
Sbjct: 241 CNRANFPGQPCTSEHLQVLVRAIPIKEGHKLRIIWPITPGIHHYKEGPCRYLSHLIGHEG 300
Query: 301 EGSLYYVLKTLGWATWLSADESSFTMNLSFFEVVIDLTDVGQEHMQDVIGLLFKYISLLK 360
EGSLYYVLKTLGWAT LSA ES F+MN SFF+VVI+LTDVGQEHMQDVIGLLFKYISLLK
Sbjct: 301 EGSLYYVLKTLGWATGLSAGESIFSMNFSFFQVVINLTDVGQEHMQDVIGLLFKYISLLK 360
Query: 361 QSGIWQWIFDELSAICETKFHYADKIDPIDYVVNLSSSMQLYPPEDWLVGSSLPSKFNPK 420
QSGI QWIFDELSAICETKFHY DKI PIDYVV+LSS+MQLYPPEDWLVGSSLPSKFNPK
Sbjct: 361 QSGICQWIFDELSAICETKFHYTDKIRPIDYVVDLSSNMQLYPPEDWLVGSSLPSKFNPK 420
Query: 421 LIGTVLDQLSVDNVRIFWESKKFEGKTDKVEKWYGTAYSIEKVSGPLVQDWMQSAPDVKL 480
LIGTVLDQLSVDNVRIFWESKKFEGK DKVEKWYGTAYSIEKVSG LVQDWMQSAPDV L
Sbjct: 421 LIGTVLDQLSVDNVRIFWESKKFEGKMDKVEKWYGTAYSIEKVSGALVQDWMQSAPDVNL 480
Query: 481 HLPASNIFIPTDLSPKCACEKVKFPVLLKKSSYSSIWYKPDTMFSTPKAYVKIDFICPHA 540
HLPASNIFIPTDLS K ACEKVKFPVLL+KSSYSSIWYKPDTMFSTPKAYVKIDFICPHA
Sbjct: 481 HLPASNIFIPTDLSLKLACEKVKFPVLLRKSSYSSIWYKPDTMFSTPKAYVKIDFICPHA 540
Query: 541 DISPEAEVLTAIFTRLLVDYLNEYAYYAEVAGLYYGINLVESGFQVSLNGYNHKLRILLE 600
DISPEAEVLT IFT LLVDYLNEYAYYA VAGL YGIN V+SGFQV+LNGYNHKLRILLE
Sbjct: 541 DISPEAEVLTQIFTMLLVDYLNEYAYYAVVAGLSYGINAVDSGFQVTLNGYNHKLRILLE 600
Query: 601 TIVTKIANFSVKPDRFLVIKETLLKNYQNFKFQQPYQQAFYYCSLILGDRTWPLMDKLAI 660
TIVTKIANFSVKPDRFLVIK LL + + PY QA YCSLILGD+ WPLMD+LAI
Sbjct: 601 TIVTKIANFSVKPDRFLVIK-VLLTSKARXQVXSPYLQALDYCSLILGDQKWPLMDELAI 660
Query: 661 LSNLGAQDLDKFVPTLLSSAYLECFIAGNIERTEAESMIDHIEDKFFKGSNPISRPLHPS 720
L +LGA+DLDKFVPTLLSSA LECFIAGNIERTEAESMIDHIEDKFFKG NPISRPL+PS
Sbjct: 661 LPHLGARDLDKFVPTLLSSANLECFIAGNIERTEAESMIDHIEDKFFKGPNPISRPLYPS 720
Query: 721 QYPPNRIVKLERSIGYFYSAEGLNSNNENSALVHYIQVHRDEFLQNVKLQLFAHVAQQAA 780
QYP NRIVKLER IGYFYSAEGLNSNNENSALVHYIQVHRDEFL NVKLQLFAHVAQQAA
Sbjct: 721 QYPANRIVKLERGIGYFYSAEGLNSNNENSALVHYIQVHRDEFLPNVKLQLFAHVAQQAA 780
Query: 781 FHQLRSVEQLGYITALAQRNDCGIRGVQFIIQSTVKGPRDIDLRVEAFLEMFEKKLVEMT 840
FHQLR+VEQLGYITAL QRN G+RGVQFIIQSTVKGPRDIDLRVEAFLEMFEKKLVEMT
Sbjct: 781 FHQLRTVEQLGYITALVQRNHRGVRGVQFIIQSTVKGPRDIDLRVEAFLEMFEKKLVEMT 840
Query: 841 VDEFQSNVNALVDAKLEKFKNLREEARFYWGEISEGTLKFDRRESEV 888
VDEFQ+NVNALVDAKLEKFKNL+EE+RFYWGEIS GTLKFDRRESEV
Sbjct: 841 VDEFQNNVNALVDAKLEKFKNLKEESRFYWGEISGGTLKFDRRESEV 886
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| IDE1_ARATH | 0.0e+00 | 68.98 | Insulin-degrading enzyme-like 1, peroxisomal OS=Arabidopsis thaliana GN=PXM16 PE... | [more] |
| IDE2_ARATH | 0.0e+00 | 65.22 | Insulin-degrading enzyme-like 2 OS=Arabidopsis thaliana GN=At3g57470 PE=2 SV=2 | [more] |
| IDE_BOVIN | 1.9e-199 | 39.25 | Insulin-degrading enzyme OS=Bos taurus GN=IDE PE=2 SV=1 | [more] |
| IDE_HUMAN | 3.7e-198 | 39.04 | Insulin-degrading enzyme OS=Homo sapiens GN=IDE PE=1 SV=4 | [more] |
| IDE_RAT | 5.3e-197 | 39.11 | Insulin-degrading enzyme OS=Rattus norvegicus GN=Ide PE=1 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A061EXW9_THECC | 0.0e+00 | 75.70 | Insulinase (Peptidase family M16) family protein isoform 1 OS=Theobroma cacao GN... | [more] |
| D7THW7_VITVI | 0.0e+00 | 74.97 | Putative uncharacterized protein OS=Vitis vinifera GN=VIT_08s0007g08490 PE=3 SV=... | [more] |
| A0A0D2Q6F9_GOSRA | 0.0e+00 | 74.61 | Uncharacterized protein OS=Gossypium raimondii GN=B456_002G147300 PE=3 SV=1 | [more] |
| A0A067JQ91_JATCU | 0.0e+00 | 73.17 | Uncharacterized protein OS=Jatropha curcas GN=JCGZ_21090 PE=3 SV=1 | [more] |
| M5VTN9_PRUPE | 0.0e+00 | 73.22 | Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa000903mg PE=3 SV=1 | [more] |
