Cucsa.327990 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.327990 vs. Swiss-Prot
Match: NOP14_MOUSE (Nucleolar protein 14 OS=Mus musculus GN=Nop14 PE=1 SV=2)

HSP 1 Score: 292.7 bits (748), Expect = 1.3e-77
Identity = 238/903 (26.36%), Postives = 421/903 (46.62%), Query Frame = 1

Query: 14  KKGKKKKKKSSGPKALTMKVSAPKANPFESIWSHRKFDVLGKKRKGEERRIGLARSLAIE 73
           K+   +++    P   +   +    NPFE   + +KF +LG+K + +    G++R+ AI 
Sbjct: 5   KRTGARRQVHKAPAGASGGPAKTNPNPFEVKVNRQKFQILGRKTRHDVGLPGVSRARAIR 64

Query: 74  KRKKTLLKEYERSRKSTEFSDKRIGEWDEELGEFDKAILRSQRELKRKLSKSSKFNLSDG 133
           KR +TLLKEY+   KS  F+DKR GE++  +   +K + R   E +R   K + +NL++ 
Sbjct: 65  KRTQTLLKEYKERNKSNVFADKRFGEYNSNISPEEKMMKRFALEQQRYHEKKNIYNLNED 124

Query: 134 EEDDYFGTQNLGALPANDDFEDEIIPDDDED----EAAAAETNKGAYRDTQQKGRLLEGE 193
           EE  ++G Q+L  +  ++D  D     +D      E  A+    G ++++ QK    EGE
Sbjct: 125 EELTHYG-QSLADIEKHNDIVDSDSDTEDRGALSAELTASHFGGGVHKNSSQK----EGE 184

Query: 194 DAVRLLAVYSFPFYFIKRKSKKEVMEEVIAKSKFFKAQKAKDKEENEQLVEELDKKFETL 253
           D               K K++KE++EE+IAKSK  K ++   +E+  +L E+LD+ ++ +
Sbjct: 185 DG-------------DKPKTRKELIEELIAKSKQEKRERQAQREDALELTEKLDQDWKEI 244

Query: 254 VQSEALLSLTGSGNSNALKALVQKSVSNEHLKKDNLPAAGKTETFNQE-------ERQKR 313
              + L+S              +K   +E  +K   P   + +   +E       +   R
Sbjct: 245 ---QILMS--------------RKPKKSEDKEKKEKPQPDEYDMMVRELGFEMKAQPSNR 304

Query: 314 MLAPDNSSDEEDD-----DAE--TAFVGKQ--------NYISGDDLGDSFTLDDERNHKK 373
           M   +  + EE +     +AE     +GK          + S DDL D F LD +     
Sbjct: 305 MKTEEELAKEEQERLKKLEAERLRRMLGKDEHENKKKPKHTSADDLNDGFILDKDDRRLL 364

Query: 374 GWVDDILRRKDADGTESEGDDSAEDSDDSQDDEDGDSDDESEEDDSNHGVKQSLKDWEQS 433
            + D  +  +D    +S+         D Q+++  + +D+SEE+D +H   +  +D +  
Sbjct: 365 SYKDGKMNIEDVQEEQSK-------EADGQENDQKEGEDDSEEEDESHEDSEESEDPDSH 424

Query: 434 DDDILDTESEDDDEASEGGKQQDEVHPKGKV--DHEGPKKAHKRSI----AKSKSFDQFL 493
            D   + ESE+++E     K++    P GK+  D +  +KA    +    A  +SF++  
Sbjct: 425 SDLESNIESEEENETP---KKEQRQTPGGKLPKDDQKAQKAVAAELPYVFAAPESFEELK 484

Query: 494 SLLANCSNSDIILIIGRIRASNAIQLTDKNLEKMQRFYGILLQYFAVSANKKPLNVELLN 553
            LL+  S  + +L++ RI+  N   L   N  K+++ +G LLQY    A     +++ ++
Sbjct: 485 FLLSGRSMEEQLLVVERIQKCNHPSLAVGNKAKLEKLFGFLLQYIGDLATDSTPDLKTID 544

Query: 554 LLFKPLMEMSMEIPFYAATCARMRISHTHQHFCVQNKSPENSLWPSSKTLILLRLWTMIF 613
            L   L  +    P  A+   R  +           ++   + +P    LI L++  ++F
Sbjct: 545 KLVVHLYSLCQMFPESASDSIRFVLRDAMHEMEEMIETKGRAAFPGLDVLIYLKITGLLF 604

Query: 614 PCSDYNHVVITPTILLMCEYLMRCPILTCRDIAIGAFLCSLLLSVARQSSKFCPEAINFL 673
           P SD+ H V+TP +L M + L +CP+++ +D+  G F+C L L     S +F PE  NFL
Sbjct: 605 PTSDFWHPVVTPALLCMSQMLTKCPVMSLQDVIKGLFVCCLFLEYVSLSRRFIPELFNFL 664

Query: 674 RTLLAAAVSSSSSSQNPQICHLVDSQALGK---LLHIQNPTNEIT------PLDFFFIMN 733
             +L  A + ++ SQ   + H    +ALGK   LL + +  +  T      PL +    N
Sbjct: 665 LGILYIA-TPNTKSQGSTLVH--PFRALGKNSELLVVSDKADVTTWQRGSLPLHW---AN 724

Query: 734 LNEDSLVFSSDNFRAGLLSTVTETLDGFVNIYGQLKSFPEIFLPISTILHELAQQENMPD 793
                    +++ R   +++    +   V +Y  L SF  IF P   +L +     ++P 
Sbjct: 725 RLSTLTATEANHTRLSCVASCLSLMKHCVLMYQALPSFHAIFRPHQALLSKHLADCSLPQ 784

Query: 794 VLQNKFRKVAEAIEAKTEEHYMGRQPLRMRKQKTAPIKLLNPKFEENFVRGRDYDPDRER 853
            LQ   + +  A+E + +      +PL   K K  P+K   P+  +    GR     +E 
Sbjct: 785 ELQELAQSILSAMEGQKQH----CRPLVCEKSKPVPLKQFTPRLVKVLEFGRKQGSSKEE 844

Query: 854 AERRKMQKLLKRETKGAARELRKDNHFLSEVKARDKAKQDEERAEKYKKARTFLEAQEHA 876
            ER+++    KRE KGA RE+RKDN FL+ ++  +  ++D ER  K K+    L  QE  
Sbjct: 845 QERKRLIHKHKREFKGAVREIRKDNQFLARMQLSEIMERDAERKRKVKQLFNSLATQEGE 852

BLAST of Cucsa.327990 vs. Swiss-Prot
Match: NOP14_HUMAN (Nucleolar protein 14 OS=Homo sapiens GN=NOP14 PE=1 SV=3)

HSP 1 Score: 284.6 bits (727), Expect = 3.6e-75
Identity = 248/904 (27.43%), Postives = 416/904 (46.02%), Query Frame = 1

Query: 14  KKGKKKKKKSSGPKALTMKVSAPKANPFESIWSHRKFDVLGKKRKGEERRIGLARSLAIE 73
           KK   ++K S  P       +   +NPFE   + +KF +LG+K + +    G++R+ A+ 
Sbjct: 5   KKVGARRKASGAPAGARGGPAKANSNPFEVKVNRQKFQILGRKTRHDVGLPGVSRARALR 64

Query: 74  KRKKTLLKEYERSRKSTEFSDKRIGEWDEELGEFDKAILRSQRELKRKLSKSSKFNLSDG 133
           KR +TLLKEY+   KS  F DKR GE++  +   +K + R   E +R   K S +NL++ 
Sbjct: 65  KRTQTLLKEYKERDKSNVFRDKRFGEYNSNMSPEEKMMKRFALEQQRHHEKKSIYNLNED 124

Query: 134 EEDDYFGTQNLGALPANDDFEDEIIPDDDED----EAAAAETNKGA---YRDTQQKGRLL 193
           EE  ++G Q+L  +  ++D  D     +D      E  AA    G    ++ TQQ+G   
Sbjct: 125 EELTHYG-QSLADIEKHNDIVDSDSDAEDRGTLSAELTAAHFGGGGGLLHKKTQQEGEER 184

Query: 194 EGEDAVRLLAVYSFPFYFIKRKSKKEVMEEVIAKSKFFKAQKAKDKEENEQLVEELDKKF 253
           E                  K KS+KE++EE+IAKSK  K ++   +E+  +L E+LD+ +
Sbjct: 185 E------------------KPKSRKELIEELIAKSKQEKRERQAQREDALELTEKLDQDW 244

Query: 254 ETLVQSEALLSLTGSGNSNALKALVQKSVSNEHLKKDNLPAAGKTETFNQEERQKRMLAP 313
           + +   + LLS               K+  +E+  K   P   K + ++   R+      
Sbjct: 245 KEI---QTLLS--------------HKTPKSENRDKKEKP---KPDAYDMMVRELGFEMK 304

Query: 314 DNSSDEEDDDAETAFVGKQNY--ISGDDLGDSFTLDDERNHKKG---WVDDILRRKDADG 373
              S+    +AE A   +++   +  + L      D++ N KK      DD+      DG
Sbjct: 305 AQPSNRMKTEAELAKEEQEHLRKLEAERLRRMLGKDEDENVKKPKHMSADDL-----NDG 364

Query: 374 TESEGDDS------------AEDSDDSQDDEDGDSDDESEEDDSNHGVKQSLKDWEQSD- 433
              + DD              ED  + Q  E  D +   EE DS+ G     +D E+SD 
Sbjct: 365 FVLDKDDRRLLSYKDGKMNVEEDVQEEQSKEASDPESNEEEGDSSGG-----EDTEESDS 424

Query: 434 -DDILDTES--EDDDEASEGGKQQDEVHPKGKVDHEGPKKAHKR-------SIAKSKSFD 493
            D  LD ES  E ++E  +  K+Q +   KG +   G ++A K        + A  +S++
Sbjct: 425 PDSHLDLESNVESEEENEKPAKEQRQTPGKGLI--SGKERAGKATRDELPYTFAAPESYE 484

Query: 494 QFLSLLANCSNSDIILIIGRIRASNAIQLTDKNLEKMQRFYGILLQYFAVSANKKPLNVE 553
           +  SLL   S  + +L++ RI+  N   L + N  K+++ +G LL+Y    A   P ++ 
Sbjct: 485 ELRSLLLGRSMEEQLLVVERIQKCNHPSLAEGNKAKLEKLFGFLLEYVGDLATDDPPDLT 544

Query: 554 LLNLLFKPLMEMSMEIPFYAATCARMRISHTHQHFCVQNKSPENSLWPSSKTLILLRLWT 613
           +++ L   L  +    P  A+   +  +           ++   +  P    LI L++  
Sbjct: 545 VIDKLVVHLYHLCQMFPESASDAIKFVLRDAMHEMEEMIETKGRAALPGLDVLIYLKITG 604

Query: 614 MIFPCSDYNHVVITPTILLMCEYLMRCPILTCRDIAIGAFLCSLLLSVARQSSKFCPEAI 673
           ++FP SD+ H V+TP ++ + + L +CPIL+ +D+  G F+C L L     S +F PE I
Sbjct: 605 LLFPTSDFWHPVVTPALVCLSQLLTKCPILSLQDVVKGLFVCCLFLEYVALSQRFIPELI 664

Query: 674 NFLRTLLAAAVSSSSSSQNPQICHLVDSQALGK---LLHIQN----PTNEITPLDFFFIM 733
           NFL  +L  A + + +SQ   + H    +ALGK   LL +       T + + L   +  
Sbjct: 665 NFLLGILYIA-TPNKASQGSTLVH--PFRALGKNSELLVVSAREDVATWQQSSLSLRWAS 724

Query: 734 NLNEDSLVFSSDNFRAGLLSTVTETLDGFVNIYGQLKSFPEIFLPISTILHELAQQENMP 793
            L   +    +++ R   L+     L   V +YG L SF  I  P+  +L +     + P
Sbjct: 725 RLRAPTST-EANHIRLSCLAVGLALLKRCVLMYGSLPSFHAIMGPLQALLTDHLADCSHP 784

Query: 794 DVLQNKFRKVAEAIEAKTEEHYMGRQPLRMRKQKTAPIKLLNPKFEENFVRGRDYDPDRE 853
             LQ    ++ ++   + E      +PL   K K  P+KL  P+  +    GR     +E
Sbjct: 785 QELQ----ELCQSTLTEMESQKQLCRPLTCEKSKPVPLKLFTPRLVKVLEFGRKQGSSKE 844

Query: 854 RAERRKMQKLLKRETKGAARELRKDNHFLSEVKARDKAKQDEERAEKYKKARTFLEAQEH 876
             ER+++    KRE KGA RE+RKDN FL+ ++  +  ++D ER  K K+    L  QE 
Sbjct: 845 EQERKRLIHKHKREFKGAVREIRKDNQFLARMQLSEIMERDAERKRKVKQLFNSLATQEG 849

BLAST of Cucsa.327990 vs. Swiss-Prot
Match: NOP14_DROME (Nucleolar protein 14 homolog OS=Drosophila melanogaster GN=l(3)07882 PE=2 SV=2)

HSP 1 Score: 206.8 bits (525), Expect = 9.5e-52
Identity = 220/900 (24.44%), Postives = 396/900 (44.00%), Query Frame = 1

Query: 18  KKKKKSSGPKALTMKVSAPKANPFESIWSHRKFDVLGKKRKGEERRIGLARSLAIEKRKK 77
           KK  +S+ P   +   S+ + NPF+   +  KF +LG+  K +    G++R+ A++KR +
Sbjct: 16  KKTTRSANPFDNSTAQSSKRGNPFDVHVNKEKFKILGRICKHDRGMPGVSRAKALQKRAQ 75

Query: 78  TLLKEYERSRKSTEFSDKRIGEW--DEELGE--FDKAILRSQRELKRKLSKSSKFNLSDG 137
           TL +++    K+  F D RIG+    ++L E   +   L  +    R   K+ KFNL+D 
Sbjct: 76  TLGQQFAVKHKTNRFKDNRIGKHLSGDQLTESVMNARYLAEKMSQVRSNQKAEKFNLNDD 135

Query: 138 EEDDYFGTQNLGALPANDDFEDEIIPDDD-EDEAAAAETNKGAYRDTQQKGRLLEGEDAV 197
           E   + G      L   + + DE   D++ +DEA  A+    A+          EG+ A 
Sbjct: 136 ELLTHRGQ----TLEEIEQYRDERSDDEELDDEALDADFTAAAHFGG-------EGDSA- 195

Query: 198 RLLAVYSFPFYFIKRKSKKEVMEEVIAKSKFFKAQKAKDKEE----------NEQLVEEL 257
                          + ++  ++E+I + K  K + AK+K+E          N +L+  L
Sbjct: 196 ---------------QDRQAAIDEMIVEQKRRKNEIAKEKDEVYDLTEKLDANYKLLLPL 255

Query: 258 DKKFETLVQSEALLSLTGSGNSNALKALV---QKSVSNEHLKKDNLPAAGKTETFNQEER 317
             K   + + E             LK ++   + SV+++ +  D L    K E    E+ 
Sbjct: 256 VAK---VTKDEQDAKPPPDAYDKLLKEMIFEPRGSVADKLINPDEL---AKQEAARLEKL 315

Query: 318 QKRMLAPDNSSDEEDDDAETAFVGKQNYISGDDLGDSFTLDDERNHKKGWVDDILRRKDA 377
           +   L    +  EED++A  A   K  + S DDL D + L  E +      DD L   D 
Sbjct: 316 ENERLRRMRADGEEDEEASVA---KPKHRSADDLDDGYFLAGEDDEG----DDTLAY-DL 375

Query: 378 DG---TESEGDDSAE---DSDDSQDDEDGDSDDESEEDDSNHGVKQSLKDWEQSDDDILD 437
           DG   T   G   A    D ++  DD++G+ ++E + D+ +     +L D ++S+ +   
Sbjct: 376 DGNLGTHLNGKKEAVLKGDENEDDDDKEGEEEEEEDSDEESDSEVDNLSDLKESESE--- 435

Query: 438 TESEDDDEASEGGKQQDEVHPKGKVDHEGPKKAHKRSIAKSKSFDQFLSLLANCSNSDII 497
           +E E+  +AS+   ++        +D   P      +I   K+++ F  LL+  + +   
Sbjct: 436 SEPEEAPKASKKKAKKSADTIPASLDTSIPF-----TIKMPKTYEDFTELLSKHATAQKA 495

Query: 498 LIIGRIRASNAIQLTDKNLEKMQRFYGILLQYFA---VSANKKPL--NVELLNLLFKPLM 557
           +II RI   N  +L   N E + + Y  LLQY       A+++ +  + +LL+ L   L 
Sbjct: 496 IIIERIIKCNHPKLEGVNRENVVKLYSFLLQYLKDLFEDASEQDIREHFQLLSKLMPYLY 555

Query: 558 EMSMEIPFYAATCARMRISHTHQHFCVQNKSPENSLWPSSKTLILLRLWTMIFPCSDYNH 617
           E++   P   +      I   ++ F   +K     ++PS  TL+  +L   ++  SD+ H
Sbjct: 556 ELTQLNPERMSNTLLEVIKEKYEEFRKNHK-----MYPSLDTLVYFKLVANLYSTSDFRH 615

Query: 618 VVITPTILLMCEYLMRCPILTCRDIAIGAFLCSLLLSVARQSSKFCPEAINFLRTLLAAA 677
            V+TP  + +   L R  + T ++I++G FL +++L    QS +  P   NFL+ ++  +
Sbjct: 616 PVVTPCFIFIQHVLSRSRVRTRQEISMGLFLVTVVLEFVSQSKRLVPAVFNFLQGIVHMS 675

Query: 678 VSSSSSSQNPQICHLVDSQALGKLLHIQNPTNEITPLDFFFIMNLNEDSLVFSS--DNFR 737
           +      Q            L KLL +   T E T L+      L    LV  +   +F+
Sbjct: 676 IPKRDVEQLEITPPFERDGPLSKLLALPANT-ESTKLE---PQQLQPTDLVTQTITPDFK 735

Query: 738 AGLLSTVTETLDGFVNIYGQLKSFPEIFLPISTILHELAQQENMPDVLQNKFRKVAEAIE 797
              L T    +   + +  +      +  P   +L  L   E+ P+ +    +   E  E
Sbjct: 736 VRALDTSLLLIKEALQLVEEHVGACYLAQPFLALLSRLPL-ESYPEHVHQHHKDATELAE 795

Query: 798 AKTEEHYMGRQPLRMRKQKTAPIKLLNPKFEENFVRGRDYDPDRERAERRKMQKLLKRET 857
               +     +PL   ++K   ++LL P+FE  +   R     + + ER K+   +KRE 
Sbjct: 796 KLAAQKM---KPLAPAEKKPKALRLLEPRFEAVYDDKRRPKMSKAKEERAKLLHKIKREK 851

Query: 858 KGAARELRKDNHFLSEVKARDKAKQDEERAEKYKKARTFLEAQEHAFKSGQLGNGRKRRK 887
           KGA RE+R+D  F+  ++ +   + D+ER EK K  R + EA     +  +L   +K++K
Sbjct: 856 KGAIREIRRDTSFVQMLRLKQTLQSDKERHEKVK--RIYQEASVQQGELNELSRAKKKKK 851

BLAST of Cucsa.327990 vs. Swiss-Prot
Match: NOP14_SCHPO (Probable nucleolar complex protein 14 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=nop14 PE=1 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 8.3e-48
Identity = 204/882 (23.13%), Postives = 377/882 (42.74%), Query Frame = 1

Query: 12  NDKKGKKKKKKSSGPKALTMKVSAPKANPFESIWSHRKFDVLGKKRKGEERRIGLARSLA 71
           N+KK + +  +S   +   ++      N F+  ++ RKFDV G++ KG E + G++R + 
Sbjct: 25  NNKKSRTRSTESHEDRQAKVQKIQSDFNLFDRQFTKRKFDVGGRRVKGTEGKPGVSRGVG 84

Query: 72  IEKRKKTLLKEYERSRKSTEFSDKRIGEWDEELGEFDKAILRSQRELKRKLSKSSKFNLS 131
            E R++T+  E ++  +S    D+R GE +  L   +K + R  RE +R+ SK   +NL 
Sbjct: 85  EELRRRTIGAELKKRNRSGAIIDRRFGENNPHLSVEEKMLERFSREQQRR-SKRELYNL- 144

Query: 132 DGEEDDYFGTQNLGALPANDDFEDEIIPDDDEDE---AAAAETNKGAYRDTQQKGRLLEG 191
           D E+    G + L  +   D FE+     D+ +E         + G + D+  +    EG
Sbjct: 145 DAEDVLTHGNRPLSDI---DSFEEPGFGLDEGEELNDEVVRRMHFGGFEDSDAENE-KEG 204

Query: 192 EDAVRLLAVYSFPFYFIKRKSKKEVMEEVIAKSKFFKAQKAKDKEENEQLVEELDKKFET 251
           E A                KSK+EVM E+IAKSK +KA++  +KE  E   E+LD++ E 
Sbjct: 205 EGA---------------HKSKREVMSEIIAKSKHYKAERQAEKERYEDEREKLDEQMED 264

Query: 252 LVQSEALLSLTGSGNSNALKALVQKSVSNEHLKKDNL-------PAAGKTETFNQEERQK 311
           L   ++ LS     +  +     +  +S+   + D+          A  TE    EE   
Sbjct: 265 L---QSFLSDYKKASRKSGIKTQRPIISDGDARYDSFVREMVFDKRAHPTERTKTEEELA 324

Query: 312 RMLAPDNSSDEEDDDAETAFVGKQNYISGDDLGDSFTLDDERNHKKGWVDDILRRKDADG 371
           ++ A D   + ED       + +  +   D   ++ +++DE+       D++       G
Sbjct: 325 QIEA-DRLRELEDQR-----ISRMEHYQEDSASEAGSIEDEQA-----TDNVF--GFGKG 384

Query: 372 TESEGDDS----AEDSDDSQDDEDGDSDDESEEDDSNHGVKQSLKDWEQSDDDILDTESE 431
            E E ++      E++++S+D+E  +SD    +D+                      + +
Sbjct: 385 LEQENEEEWNGINEEAEESEDEESVNSDTSFVDDE----------------------QLK 444

Query: 432 DDDEASEGGKQQDEVHPKGKVDHEGP-KKAHKRSIAKSKSFDQFLSLLANCSNSDIILII 491
            +++   G   ++E   K  + +  P   +H   +   K  D            D   ++
Sbjct: 445 VEEQPLVGSAIKNEGSEKASLAYTYPCPTSHVEFVQLLKGLDY----------KDYPTVV 504

Query: 492 GRIRASNAIQLTDKNLEKMQRFYGILLQYFAVSANKKPLNVELLNLLFKPLMEMSMEIPF 551
            RIR  + ++L   N  +++ F  ILLQ+      +  +++ELL  L + L  ++ + P 
Sbjct: 505 SRIRTLHHVKLHPDNKSRLENFSVILLQHILHLTRQPMISMELLEHLTEHLHSLAQQFPS 564

Query: 552 YAATCARMRISHTHQHFCVQNKSPENSLWPSSKTLILLRLWTMIFPCSDYNHVVITPTIL 611
                    +    +        PE   +P    L+   L   IFP SD  H+V++P +L
Sbjct: 565 ALGISFISVVEGMRKRLAKSYVYPEIK-FPEISDLLFFNLTGSIFPTSDKKHIVVSPVML 624

Query: 612 LMCEYLMRCPILTCRDIAIGAFLCSLLLSVARQSSKFCPEAINFLRTLLAAAVSSSSSSQ 671
            M E L + P  +  D+    ++ +L L     S ++ PE I         AVS +    
Sbjct: 625 TMAESLSQSPADSLSDVCKKLYIANLFLKFQSYSHRYVPEVI--------TAVSQALYLL 684

Query: 672 NPQICHLVDSQALGKLLHIQNPTNEITPLDFFFIMNLNEDS----LVFSSDNFRAGLLST 731
            P    +V             P +     + F I +++ D      ++  +    GLL +
Sbjct: 685 YPNFISIVPGT-------FALPDSLKEKQNLFAIQDISLDEPQRLSLYELEELPTGLLQS 744

Query: 732 -----VTETLDGFVNIYGQLKSFPEIFLPISTILHELA-QQENMPDVLQNKFRKVAEAIE 791
                    ++  ++IY + ++F EIF+PI  +L   + ++E +   L  K     +A+ 
Sbjct: 745 SILFITLNLIEMAIDIYFKEQAFIEIFVPIMDMLQLYSLKKELLSKRLSEKLLSTLQAVS 804

Query: 792 AKTEEHYMGRQPLRMRKQKTAPIKLLNPKFEENFVRGR-DYDPDRERAERRKMQKLLKRE 851
              E     R+PL ++  +   I    PKFEE +   +  +D D ERA+  K++   +  
Sbjct: 805 DSIESAKANRKPLALQSHRPLGITSQVPKFEEGYSLDKSSHDIDPERAQLNKLRAQHRDA 821

Query: 852 TKGAARELRKDNHFLSEVKARDKAKQDEERAEKYKKARTFLE 868
            KGA R LRKD  F++  + +++  +D+   EK +K    L+
Sbjct: 865 KKGAIRTLRKDARFIARERRQEQRAKDQAYNEKMRKLENRLQ 821

BLAST of Cucsa.327990 vs. Swiss-Prot
Match: NOP14_KLULA (Probable nucleolar complex protein 14 OS=Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) GN=NOP14 PE=3 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 1.2e-46
Identity = 228/895 (25.47%), Postives = 392/895 (43.80%), Query Frame = 1

Query: 5   SVRSSSNNDKKGKKKKKKSSGPKALTMKVSAPKANPFESIWSHRKFDVLGKKRKGEER-- 64
           +V+ SS   KKGK  ++     +  T++    + NPF+   +  K   +G  R+   +  
Sbjct: 23  NVKKSSK--KKGKSPREFDREERQKTIERIREQFNPFDVKTNRNK--KVGVMRESAAKLA 82

Query: 65  --RIGLARSLAIEKRKKTLLKEYERSRKSTEFSDKRIGEWDEELGEFDKAILRSQRELKR 124
             + G+ +    ++RK     +     +S    DKR GE D+ L   +  + R  +E +R
Sbjct: 83  VGKPGITKQAGEDQRKMFYEAKKSSKNRSGGVLDKRFGEKDKNLTSEEIMLERFTKERQR 142

Query: 125 KLS-KSSKFNLSD---GEEDDYFG---TQNLGALPANDDFEDEIIPDDDEDEAAAAETNK 184
           + S K S FNL D   GE+++ +G   T    +L   DDF++  +     D         
Sbjct: 143 QSSTKRSLFNLDDSDGGEDENVYGEKLTHYGKSLSLEDDFDEGDLGLQHSDSEDFRLKQA 202

Query: 185 GAYRDTQQKGRLLEGEDAVRLLAVYSFPFYFIKRKSKKEVMEEVIAKSKFFKAQKAKDKE 244
           G      Q G    G+DA++  A         ++K+K EVM+EVIAKSKF+K ++ K +E
Sbjct: 203 GKRNMADQYGDEGLGDDALQEPA---------RKKTKAEVMQEVIAKSKFYKHERQKAQE 262

Query: 245 ENEQLVEELDKKFETLVQSEALLSLTGSGNSNALKALVQKSVSNEHLKKDNLPAAGKTET 304
           +  Q +E LD  FE+++                L+ L +   S   +      A      
Sbjct: 263 KLVQDIEGLDDDFESIMSE--------------LRTLPKTKPSQTEISTSTDIAPDYDIK 322

Query: 305 FNQEERQKRMLAPDNSSDEEDDDAETAFVGKQNYISGDDLGDSFTLDDERNHKKGWVDDI 364
             +   +KR    D +  +E+   E  +  KQ             L+ +R  +   + +I
Sbjct: 323 VKELVLEKRAAPADRTKTDEEIKKE--YEDKQK-----------ELEQKRLDRMNGIFNI 382

Query: 365 LRRKDADGTESEGDDSAEDSDDSQDDEDGDSDDESEEDDSNHGVKQSLKDWEQSDDDI-L 424
             +KDA G E  G++  EDSD S++ E G+                 +K    SDDD+  
Sbjct: 383 EDQKDA-GVEDLGEEFWEDSD-SEEGEYGEY----------------IKAIPDSDDDVDF 442

Query: 425 DTESEDDDE--ASEGGKQQDEVHPKGKVDHEGPKKAHKRSIAKSKSFDQFLSLLANCSNS 484
           + ++ DD+E  A+  G+    V P               S+   +  D  L+ L +    
Sbjct: 443 EKDNNDDNEYDATSNGRPSARVIP---------------SVPCPQIHDDLLNFLKSYPIE 502

Query: 485 DIILIIGRIRASNAIQLTDKNLEKMQRFYGILLQYFAVSANKK-PLNVE----LLNLLFK 544
           +   ++ RI  +   +L + N EK+ +F  +LL++    A +   +NV+    L N L  
Sbjct: 503 EHPSLVKRIVKTYQPKLAEGNKEKLGKFTAVLLRHILFLAEEDYSVNVKEIASLQNELVS 562

Query: 545 PLMEMSMEIPFYAATCARMRISHTHQHFCVQNKSPENSLWPSSKTLILLRLWTMIFPCSD 604
            L  +S +     +   R  IS     F    +S    L PS   L+   +  M+F  SD
Sbjct: 563 ILKTLSEKFTIPLSEDCRNIISDIQTRF---KESQFYGLSPSD--LVFFSVVGMLFSTSD 622

Query: 605 YNHVVITPTILLMCEYLMRCPILTCRDIAIGAFLCSLLLSVARQSSKFCPEAINFLRTLL 664
           + H+VITP +LL+ E+L +    + + +  G+ L  + +   R S ++ PE   FL+T L
Sbjct: 623 HYHLVITPCLLLIAEFLEQIRFNSLQKLIFGSILVRIAIQYQRISKRYIPELTYFLQTSL 682

Query: 665 AAAVSSSSSSQNPQICHLVDSQAL-GKLLHIQN--PTNEITPLDFFFIMNLNEDSLVFSS 724
            + + S+            D+ ++ G  ++     P+ E+  L        +ED  +   
Sbjct: 683 RSLIPSTKGETLENKEKQEDNLSIVGSDINWSKVAPSLELHSL-------FSED--LEKQ 742

Query: 725 DNFRAGLLSTVTETLDGFV-NIYGQLKSFPEIFLPISTILHELAQQENMPDVLQNKFRKV 784
           ++ +  +L    E++D  + NI+  L +FPEI  P   IL+  A  E  P    +K +++
Sbjct: 743 ESIKLSVLVNNLESIDWCITNIWKDLTAFPEIINPFKDILNVAA--EVYPGT--SKPKQL 802

Query: 785 AEAIEAKTEEHYMGRQPLRMRKQKTAPIKLLNPKFEENFVRGR-DYDPDRERAERRKMQK 844
            E IE   +  +  R PL +++ K   I    PKFEENF   +  YDPD+ R+E  KM+ 
Sbjct: 803 VEKIEKLLK--FQERLPLTLQQHKPLAIPSNTPKFEENFNPDKKSYDPDKTRSELNKMKA 824

Query: 845 LLKRETKGAARELRKDNHFLSEVKARDKAKQDEERAEKYKKARTFLEAQEHAFKS 876
            LK+E K   +E+RKD  F +     +K K+  +   K  +    +  +E A K+
Sbjct: 863 QLKKERKFTMKEIRKDTRFEARQGIEEKKKEYADYHSKMARIVNQISTEEGAEKN 824

BLAST of Cucsa.327990 vs. TrEMBL
Match: B9HWW2_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0010s15000g PE=4 SV=2)

HSP 1 Score: 974.2 bits (2517), Expect = 1.1e-280
Identity = 536/974 (55.03%), Postives = 658/974 (67.56%), Query Frame = 1

Query: 1   MAKLSVRS---SSNNDKKGKKKKKKSSGPKALTMKVSAPK------ANPFESIWSHRKFD 60
           MAK S RS   SS+N K  KKKK   + P ++ MK SA        +NPFE+IWS RKFD
Sbjct: 1   MAKTSKRSRSSSSSNTKSKKKKKNSRTAPNSVAMKASAASKDNKNSSNPFETIWSRRKFD 60

Query: 61  VLGKKRKGEERRIGLARSLAIEKRKKTLLKEYERSRKSTEFSDKRIGEWDEELGEFDKAI 120
           +LGKKRKGEE RIGL+R  AIEKRKKTLLKEYE S KS+ F DKRIGE +E+LGEFDKAI
Sbjct: 61  ILGKKRKGEELRIGLSRCRAIEKRKKTLLKEYEESGKSSVFLDKRIGEQNEQLGEFDKAI 120

Query: 121 LRSQRELKRKLSKSSKFNLSDGEEDDYFGTQNLGALPANDDFEDEIIPDDDEDEAAAAET 180
           +RSQRE + K +K SK+NLSDGEEDD FG  NLG L   DDFEDEI+ DDD D+A A  T
Sbjct: 121 IRSQRERQLK-NKKSKYNLSDGEEDDDFGIPNLGPLSGQDDFEDEILSDDDGDDADADRT 180

Query: 181 NKGAYRDTQQKGRLLEGEDAVRLLAVYSFPFYFI-----KRKSKKEVMEEVIAKSKFFKA 240
           +K        K  +L      R L  +  P   +     K K+KKEVM+EVI KSKFFKA
Sbjct: 181 SK--------KPAIL------RQLNAHGLPQDAVHGEENKPKTKKEVMQEVILKSKFFKA 240

Query: 241 QKAKDKEENEQLVEELDKKFETLVQSEALLSLTGSGNSNALKALVQKSVSNEHLKKDNLP 300
           QKAKDKEENEQL+EELDK F +LVQS+AL SLT  G  NALKALV K + NEH+KKD LP
Sbjct: 241 QKAKDKEENEQLMEELDKSFTSLVQSQALSSLTEPGKMNALKALVNKDIPNEHVKKDELP 300

Query: 301 AAGKTETFNQEER-------------------QKRMLAP--------------------- 360
              K ETF Q+E+                     R   P                     
Sbjct: 301 VIQKPETFKQQEQPDSYDKLVYEMAIDSRARPSDRTKTPEEIAQKERERLEQLEEDRKKR 360

Query: 361 ----DNSSDEEDDDAETAFVGKQNYISGDDLGDSFTLDDERNHKKGWVDDILRRKDADGT 420
               D+SSDEE+DD E     +   ISGDDLGDSF+L +E    KGWVD+IL RK+AD +
Sbjct: 361 MLVADDSSDEENDDVEKLSAQRPRSISGDDLGDSFSLYEEPGTTKGWVDEILARKEADDS 420

Query: 421 ESEGDDSAEDSDDSQD--DEDGDSDDESEEDDSNHGVKQSLKDWEQSDDDILDTESEDDD 480
           ++E DDS+E+S  + D  D++G  +D+++ DD  H    SLKDWEQSDDD L T+ E+D+
Sbjct: 421 DNEDDDSSEESASANDDGDDEGSDEDDTDGDDDEHEKSTSLKDWEQSDDDNLGTDLEEDE 480

Query: 481 EASEGGKQQDEVHP-----KGKVDHEGPKKAHKRS----------------------IAK 540
           E         E+ P       K +   P+K  ++S                      I  
Sbjct: 481 EHGSHDGDDGEIEPISHKKSKKTEPVEPRKGDEKSLDGKKKKANREQHSTQPDIPHIIEA 540

Query: 541 SKSFDQFLSLLANCSNSDIILIIGRIRASNAIQLTDKNLEKMQRFYGILLQYFAVSANKK 600
            KSF++F ++L NCSN ++IL++ RIR SNAIQL  +N +K+Q FYG+LLQYFAV ANKK
Sbjct: 541 PKSFEEFCAILENCSNENVILVVDRIRKSNAIQLAAENRKKIQVFYGVLLQYFAVLANKK 600

Query: 601 PLNVELLNLLFKPLMEMSMEIPFYAATCARMRISHTHQHFCVQNKSPENSLWPSSKTLIL 660
           PLN+ELLN L KPLMEMS+EIP+++A CAR RI  T   FC   K+ ENS WPS KTL L
Sbjct: 601 PLNIELLNFLVKPLMEMSVEIPYFSAICARQRILRTRAQFCEALKNTENSSWPSMKTLSL 660

Query: 661 LRLWTMIFPCSDYNHVVITPTILLMCEYLMRCPILTCRDIAIGAFLCSLLLSVARQSSKF 720
           LRLW+MIFPCSD+ HVV+TP ILLM EYLMRCPIL+ RDIAIG+FLC+++LS+ +QS KF
Sbjct: 661 LRLWSMIFPCSDFRHVVMTPVILLMSEYLMRCPILSGRDIAIGSFLCTMVLSITKQSQKF 720

Query: 721 CPEAINFLRTLLAAAVSSSSSS-QNPQICHLVDSQALGKLLHIQNPTNEITPLDFFFIMN 780
           CPEAI FLRTLL A      SS Q  Q  HL++ + +  LLHI +  NEI PL+F  +M+
Sbjct: 721 CPEAIMFLRTLLMATTERKPSSYQESQFYHLMELKEIKPLLHIHDHVNEIRPLNFLMVMD 780

Query: 781 LNEDSLVFSSDNFRAGLLSTVTETLDGFVNIYGQLKSFPEIFLPISTILHELAQQENMPD 840
           + ED+  FSSD+FR G+L T+ ETL GFV+IY +L SFPEIFLPIS +L E+AQQENMP 
Sbjct: 781 MQEDTSFFSSDDFRVGVLVTMVETLQGFVDIYKELSSFPEIFLPISMLLLEVAQQENMPA 840

Query: 841 VLQNKFRKVAEAIEAKTEEHYMGRQPLRMRKQKTAPIKLLNPKFEENFVRGRDYDPDRER 887
            LQ+KF+ VAE I  K  +H+M R+PL+M+K+K  PIKL+ PKFEENFV+GRDYDPDRER
Sbjct: 841 TLQDKFKDVAELINKKANKHHMMRKPLQMQKKKPVPIKLVAPKFEENFVKGRDYDPDRER 900

BLAST of Cucsa.327990 vs. TrEMBL
Match: A0A061EAI4_THECC (Nop14, putative isoform 1 OS=Theobroma cacao GN=TCM_011472 PE=4 SV=1)

HSP 1 Score: 956.8 bits (2472), Expect = 1.8e-275
Identity = 522/969 (53.87%), Postives = 650/969 (67.08%), Query Frame = 1

Query: 1   MAKLSVRSSSNNDKKGKKKKKKSSGPKALTMKVSAPKANPFESIWSHRKFDVLGKKRKGE 60
           MAK S  S +   KK KKK  K SGP A++MK+ A K+NPFE+IWS RKFD+LGKKRKGE
Sbjct: 43  MAKHS-GSDAKTKKKAKKKGSKKSGPDAISMKLKAEKSNPFETIWSRRKFDILGKKRKGE 102

Query: 61  ERRIGLARSLAIEKRKKTLLKEYERSRKSTEFSDKRIGEWDEELGEFDKAILRSQRELKR 120
           E RIGL+RSLAI+KRKKTLLKEYE+S KS+ F D RIGE ++ELGEF+K I+RSQRE + 
Sbjct: 103 ELRIGLSRSLAIQKRKKTLLKEYEQSTKSSVFVDNRIGEQNDELGEFEKGIMRSQRERQL 162

Query: 121 KLSKSSKFNLSDGEEDDYFGTQNLGALPANDDFEDEIIPDDDEDEAAAAETNKGAY---- 180
           K  K SKFNLSDGE+DD F     G+LP  DDFEDEI+ DDD D+   A   + A     
Sbjct: 163 KFGKKSKFNLSDGEDDD-FDAPGFGSLPERDDFEDEILSDDDNDDRGGATNKRSAILKQL 222

Query: 181 -----RDTQQKGRLLEGEDAVRLLAVYSFPFYFIKRKSKKEVMEEVIAKSKFFKAQKAKD 240
                +D  ++G L+EGE+               K K+KKE+MEEVI KSK+FKAQKAKD
Sbjct: 223 NSHGAQDPTERG-LVEGEEN--------------KHKTKKEIMEEVILKSKYFKAQKAKD 282

Query: 241 KEENEQLVEELDKKFETLVQSEALLSLTGSGNSNALKALVQKSVSNEHLKKDNLPAAGKT 300
           KEENEQL+EELDK F +LVQS+ LLS+T  G  NALKALV K V NEHL K+ LP + + 
Sbjct: 283 KEENEQLMEELDKNFTSLVQSQVLLSMTEPGKINALKALVNKGVLNEHLNKEELPVSQRE 342

Query: 301 ETFNQ-------------------------------------------EERQKRMLAPDN 360
           E + Q                                           EERQKRMLA D 
Sbjct: 343 EAYKQEQPDSYDKLVNELVLEMRARPSDRTKTPEEIAQEEREQLERLEEERQKRMLATDY 402

Query: 361 SSDEEDDDAETAFVGKQNYISGDDLGDSFTLDDERNHKKGWVDDILRRKDADGTESEGDD 420
           SSDE+ ++ E   + +   ISGDDLGDSF LD+E   KKGWVD+IL RKD D   SE  +
Sbjct: 403 SSDEDGENVEKDPLQRPRAISGDDLGDSFALDEEPGSKKGWVDEILERKDEDENASEDSE 462

Query: 421 SAEDSDDSQDDEDGDSDDESEEDDSNHGVKQSLKDWEQSDDDILDTESEDDDEASEGGKQ 480
           SAED+        G+ +   E+DD  H    SLK WEQSDDD L T+ ++D+E  E    
Sbjct: 463 SAEDT--------GEDEGSEEDDDDEHEKTLSLKYWEQSDDDNLGTDLDEDEEEQE---H 522

Query: 481 QDEVHPKGKVDHEGPKKAHKRSIAKS------------------------------KSFD 540
            D V  +  V+ +G  K++K  + K                               +S +
Sbjct: 523 DDTVGDEEDVEQKGCNKSNKTELKKDDGQYVDAKKIKPSIKHTSTKSDIPFIFEAPRSLE 582

Query: 541 QFLSLLANCSNSDIILIIGRIRASNAIQLTDKNLEKMQRFYGILLQYFAVSANKKPLNVE 600
           +  SLL NCSN D+I+II RIR S+AI+L  +N +KMQ FYG+LLQYFAV ANKKPLN E
Sbjct: 583 ELSSLLENCSNGDVIVIINRIRKSDAIKLAAENRKKMQVFYGVLLQYFAVLANKKPLNFE 642

Query: 601 LLNLLFKPLMEMSMEIPFYAATCARMRISHTHQHFCVQNKSPENSLWPSSKTLILLRLWT 660
           LLNLL KPLME+SMEIP+++A CAR RI  T   FC   K+ EN  WP+ KTL LLRLW+
Sbjct: 643 LLNLLVKPLMELSMEIPYFSAICARQRILRTRTQFCEALKNQENGCWPTLKTLFLLRLWS 702

Query: 661 MIFPCSDYNHVVITPTILLMCEYLMRCPILTCRDIAIGAFLCSLLLSVARQSSKFCPEAI 720
           M+FPCSD+ HVV+TP ILLMCEYLMRCPI + RD+AIG+FLCS++L V +QS KFCPEAI
Sbjct: 703 MVFPCSDFRHVVMTPAILLMCEYLMRCPITSGRDVAIGSFLCSMVLMVTKQSRKFCPEAI 762

Query: 721 NFLRTLLAAAVSSS-SSSQNPQICHLVDSQALGKLLHIQNPTNEITPLDFFFIMNLNEDS 780
            FLRTLL AA     ++ Q+ Q  +L++ +AL  LL + +  +EI PL+F  +M++ +DS
Sbjct: 763 MFLRTLLMAATDQKLAAEQDCQFYNLMELKALRPLLRVHDCVDEINPLNFLMVMDMPDDS 822

Query: 781 LVFSSDNFRAGLLSTVTETLDGFVNIYGQLKSFPEIFLPISTILHELAQQENMPDVLQNK 840
             FSSDNFRA  L TV ETL GFV IY  L SFPEIFLPI+T+L E++QQ+++P+ L++K
Sbjct: 823 SFFSSDNFRASALVTVIETLRGFVEIYDGLNSFPEIFLPIATLLLEVSQQKHIPEALKDK 882

Query: 841 FRKVAEAIEAKTEEHYMGRQPLRMRKQKTAPIKLLNPKFEENFVRGRDYDPDRERAERRK 887
           F  VA+ I+ K +E +  R+PL++RKQK  PIKLLNPKFEENFV+GRDYDPDRE+AERRK
Sbjct: 883 FNDVAQLIKQKADEAHRLRRPLQIRKQKPVPIKLLNPKFEENFVKGRDYDPDREQAERRK 942

BLAST of Cucsa.327990 vs. TrEMBL
Match: A0A061EH40_THECC (Nop14, putative isoform 2 OS=Theobroma cacao GN=TCM_011472 PE=4 SV=1)

HSP 1 Score: 954.9 bits (2467), Expect = 6.9e-275
Identity = 521/970 (53.71%), Postives = 649/970 (66.91%), Query Frame = 1

Query: 1   MAKLSVRSSSNNDKKGKKKKKKSSGPKALTMKVSAPKANPFESIWSHRKFDVLGKKRKGE 60
           MAK S  S +   KK KKK  K SGP A++MK+ A K+NPFE+IWS RKFD+LGKKRKGE
Sbjct: 43  MAKHS-GSDAKTKKKAKKKGSKKSGPDAISMKLKAEKSNPFETIWSRRKFDILGKKRKGE 102

Query: 61  ERRIGLARSLAIEKRKKTLLKEYERSRKSTEFSDKRIGEWDEELGEFDKAILRSQRELKR 120
           E RIGL+RSLAI+KRKKTLLKEYE+S KS+ F D RIGE ++ELGEF+K I+RSQRE + 
Sbjct: 103 ELRIGLSRSLAIQKRKKTLLKEYEQSTKSSVFVDNRIGEQNDELGEFEKGIMRSQRERQL 162

Query: 121 KLSKSSKFNLSDGEEDDYFGTQNLGALPANDDFEDEIIPDDDEDEAAAAETNKGAY---- 180
           K  K SKFNLSDGE+DD F     G+LP  DDFEDEI+ DDD D+   A   + A     
Sbjct: 163 KFGKKSKFNLSDGEDDD-FDAPGFGSLPERDDFEDEILSDDDNDDRGGATNKRSAILKQL 222

Query: 181 -----RDTQQKGRLLEGEDAVRLLAVYSFPFYFIKRKSKKEVMEEVIAKSKFFKAQKAKD 240
                +D  ++G L+EGE+               K K+KKE+MEEVI KSK+FKAQKAKD
Sbjct: 223 NSHGAQDPTERG-LVEGEEN--------------KHKTKKEIMEEVILKSKYFKAQKAKD 282

Query: 241 KEENEQLVEELDKKFETLVQSEALLSLTGSGNSNALKALVQKSVSNEHLKKDNLPAAGKT 300
           KEENEQL+EELDK F +LVQS+ LLS+T  G  NALKALV K V NEHL K+ LP + + 
Sbjct: 283 KEENEQLMEELDKNFTSLVQSQVLLSMTEPGKINALKALVNKGVLNEHLNKEELPVSQRE 342

Query: 301 ETFNQ-------------------------------------------EERQKRMLAPDN 360
           E + Q                                           EERQKRMLA D 
Sbjct: 343 EAYKQEQPDSYDKLVNELVLEMRARPSDRTKTPEEIAQEEREQLERLEEERQKRMLATDY 402

Query: 361 SSDEEDDDAETAFVGKQNYISGDDLGDSFTLDDERNHKKGWVDDILRRKDADGTESEGDD 420
           SSDE+ ++ E   + +   ISGDDLGDSF LD+E   KKGWVD+IL RKD D   SE  +
Sbjct: 403 SSDEDGENVEKDPLQRPRAISGDDLGDSFALDEEPGSKKGWVDEILERKDEDENASEDSE 462

Query: 421 SAEDSDDSQDDEDGDSDDESEEDDSNHGVKQSLKDWEQSDDDILDTESEDDDEASEGGKQ 480
           SAED+        G+ +   E+DD  H    SLK WEQSDDD L T+ ++D+E  E    
Sbjct: 463 SAEDT--------GEDEGSEEDDDDEHEKTLSLKYWEQSDDDNLGTDLDEDEEEQE---H 522

Query: 481 QDEVHPKGKVDHEGPKKAHKRSIAKS------------------------------KSFD 540
            D V  +  V+ +G  K++K  + K                               +S +
Sbjct: 523 DDTVGDEEDVEQKGCNKSNKTELKKDDGQYVDAKKIKPSIKHTSTKSDIPFIFEAPRSLE 582

Query: 541 QFLSLLANCSNSDIILIIGRIRASNAIQLTDKNLEKMQRFYGILLQYFAVSANKKPLNVE 600
           +  SLL NCSN D+I+II RIR S+AI+L  +N +KMQ FYG+LLQYFAV ANKKPLN E
Sbjct: 583 ELSSLLENCSNGDVIVIINRIRKSDAIKLAAENRKKMQVFYGVLLQYFAVLANKKPLNFE 642

Query: 601 LLNLLFKPLMEMSMEIPFYAATCARMRISHTHQHFCVQNKSPENSLWPSSKTLILLRLWT 660
           LLNLL KPLME+SMEIP+++A CAR RI  T   FC   K+ EN  WP+ KTL LLRLW+
Sbjct: 643 LLNLLVKPLMELSMEIPYFSAICARQRILRTRTQFCEALKNQENGCWPTLKTLFLLRLWS 702

Query: 661 MIFPCSDYNHVVITPTILLMCEYLMRCPILTCRDIAIGAFLCSLLLSVARQSSKFCPEAI 720
           M+FPCSD+ HVV+TP ILLMCEYLMRCPI + RD+AIG+FLCS++L V +QS KFCPEAI
Sbjct: 703 MVFPCSDFRHVVMTPAILLMCEYLMRCPITSGRDVAIGSFLCSMVLMVTKQSRKFCPEAI 762

Query: 721 NFLRTLLAAAVSSSSSSQN--PQICHLVDSQALGKLLHIQNPTNEITPLDFFFIMNLNED 780
            FLRTLL AA     +++    Q  +L++ +AL  LL + +  +EI PL+F  +M++ +D
Sbjct: 763 MFLRTLLMAATDQKLAAEQDCQQFYNLMELKALRPLLRVHDCVDEINPLNFLMVMDMPDD 822

Query: 781 SLVFSSDNFRAGLLSTVTETLDGFVNIYGQLKSFPEIFLPISTILHELAQQENMPDVLQN 840
           S  FSSDNFRA  L TV ETL GFV IY  L SFPEIFLPI+T+L E++QQ+++P+ L++
Sbjct: 823 SSFFSSDNFRASALVTVIETLRGFVEIYDGLNSFPEIFLPIATLLLEVSQQKHIPEALKD 882

Query: 841 KFRKVAEAIEAKTEEHYMGRQPLRMRKQKTAPIKLLNPKFEENFVRGRDYDPDRERAERR 887
           KF  VA+ I+ K +E +  R+PL++RKQK  PIKLLNPKFEENFV+GRDYDPDRE+AERR
Sbjct: 883 KFNDVAQLIKQKADEAHRLRRPLQIRKQKPVPIKLLNPKFEENFVKGRDYDPDREQAERR 942

BLAST of Cucsa.327990 vs. TrEMBL
Match: A0A0D2W0W9_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_012G185400 PE=4 SV=1)

HSP 1 Score: 937.6 bits (2422), Expect = 1.1e-269
Identity = 520/961 (54.11%), Postives = 648/961 (67.43%), Query Frame = 1

Query: 1   MAKLSVRSSSNNDKKGKKKKKKSSGPKALTMKVSAPKANPFESIWSHRKFDVLGKKRKGE 60
           MAK S  + +   KK  KK  K S P  ++MK+ + K NPFE+IWS RKFD+LGKKRKGE
Sbjct: 1   MAKQST-AGAKTKKKSNKKHHKKSDPDVISMKLKSQKPNPFETIWSRRKFDILGKKRKGE 60

Query: 61  ERRIGLARSLAIEKRKKTLLKEYERSRKSTEFSDKRIGEWDEELGEFDKAILRSQRELKR 120
           ERRIG ARSLAI+KRKKTLLKEYE+S KS+ F DKRIGE +++LGEF+K ILRSQRE + 
Sbjct: 61  ERRIGRARSLAIQKRKKTLLKEYEQSTKSSVFVDKRIGEQNDDLGEFEKGILRSQRERQL 120

Query: 121 KLSKSSKFNLSDGEEDDYFGTQNLGALPANDDFEDEIIPDDDE--DEAAAA---ETNKGA 180
           KL K SKFNLSDGEED+ F     G+LP  DDFEDE++ DDD   DE  +      N  +
Sbjct: 121 KLGKKSKFNLSDGEEDE-FDAPEFGSLPERDDFEDEMLSDDDNYADEKRSTVLKYLNSHS 180

Query: 181 YRDTQQKGRLLEGEDAVRLLAVYSFPFYFIKRKSKKEVMEEVIAKSKFFKAQKAKDKEEN 240
            +D  + G L+EGE+               K KSKKE+MEEVI KSKFFKAQKA+DKEEN
Sbjct: 181 AKDPLE-GDLIEGEEN--------------KHKSKKEIMEEVILKSKFFKAQKARDKEEN 240

Query: 241 EQLVEELDKKFETLVQSEALLSLTGSGNSNALKALVQKSVSNEHLKKDNLPAAGKTETFN 300
           EQL++ELDK F +LVQS+ALLSLT  G  NALKALV KS+ +EH+KK+ L  A K+ET N
Sbjct: 241 EQLMDELDKSFSSLVQSQALLSLTEPGKMNALKALVNKSIPDEHVKKEELAVARKSETNN 300

Query: 301 Q-------------------------------------------EERQKRMLAPDNSSDE 360
           Q                                           EERQKRMLA D SSDE
Sbjct: 301 QEQPDSYDKLVHEMVLDMRARPSDRTKTPEEIAQEERERLERLEEERQKRMLATDYSSDE 360

Query: 361 EDDDAETAFVGKQNYISGDDLGDSFTLDDERNHKKGWVDDILRRKDADGTESEGDDSAED 420
           + ++AE  +  +   ISGDDLGDSF LDDE  +KKGWVD+IL RKDA+ +E E +D +ED
Sbjct: 361 DGENAEKDYAQRPRAISGDDLGDSFALDDEPGNKKGWVDEILERKDANDSEDEDEDDSED 420

Query: 421 SDDSQDDEDGDSDDESEEDDSNHGVKQ-SLKDWEQSDDDILDTESEDDDEASEGGKQ--Q 480
              ++D ++ +  +E EEDD N   K  SLKDWEQSDD+ + T+ E+D+E  E  +    
Sbjct: 421 LGSAEDTDEDEESEEEEEDDENECEKTLSLKDWEQSDDNNVGTDLEEDEETDEHDEAIGD 480

Query: 481 DEVHPKGK--------------VDHEGPKKAHKRSIAKSK-----SFDQFLSLLANCS-- 540
           ++V  K +              VD + PK + K +  K          + L  L++    
Sbjct: 481 EDVDKKSRNKTNKTELKKCVESVDAKKPKASGKHTSTKLDIPFIIEAPKNLEELSSLLEN 540

Query: 541 --NSDIILIIGRIRASNAIQLTDKNLEKMQRFYGILLQYFAVSANKKPLNVELLNLLFKP 600
             N D+I+II RIRASNAI+L  +N +KMQ FYG+LLQYFAV ANKKPLN EL NLL KP
Sbjct: 541 HSNDDVIVIINRIRASNAIKLAAENRKKMQVFYGVLLQYFAVLANKKPLNFELSNLLVKP 600

Query: 601 LMEMSMEIPFYAATCARMRISHTHQHFCVQNKSPENSLWPSSKTLILLRLWTMIFPCSDY 660
           +MEMS EIPF++A CAR RI  T   FC   K+ EN  WP+ KTL LLRLW+MIFPCSDY
Sbjct: 601 IMEMSTEIPFFSAICARERILRTRVQFCEALKNHENGCWPTLKTLFLLRLWSMIFPCSDY 660

Query: 661 NHVVITPTILLMCEYLMRCPILTCRDIAIGAFLCSLLLSVARQSSKFCPEAINFLRTLLA 720
            HVV TP +LLMCEYLMRCPI++ RD+AIG+FLCS++L   +QS KFCPEAI FLRTLL 
Sbjct: 661 RHVVTTPALLLMCEYLMRCPIMSGRDVAIGSFLCSMILMFTKQSRKFCPEAIMFLRTLLM 720

Query: 721 AAVSSS-SSSQNPQICHLVDSQALGKLLHIQNPTNEITPLDFFFIMNLNEDSLVFSSDNF 780
           AA     +S Q+ Q  H ++ +AL  LL I +  +EI PL+F  +M +++ S  F SDNF
Sbjct: 721 AATDHKLASEQDSQFYHFMELKALRPLLCIHDGVDEINPLNFLMVMEMSDYSSFFCSDNF 780

Query: 781 RAGLLSTVTETLDGFVNIYGQLKSFPEIFLPISTILHELAQQENMPDVLQNKFRKVAEAI 840
           RA  L TV ETL GF+ IY  L SFPEIFLPI+T+L E+++Q++MP  L++KF  V++ I
Sbjct: 781 RASALLTVIETLRGFIEIYDGLNSFPEIFLPIATLLVEVSEQKHMPKALKDKFNNVSQLI 840

Query: 841 EAKTEEHYMGRQPLRMRKQKTAPIKLLNPKFEENFVRGRDYDPDRERAERRKMQKLLKRE 887
           + K  E +  R+PL++RKQK APIKLLNPKFEENFV+GRDYDPDRERAERRK+QKL+KRE
Sbjct: 841 KKKAGETHTLRRPLQLRKQKPAPIKLLNPKFEENFVKGRDYDPDRERAERRKLQKLIKRE 900

BLAST of Cucsa.327990 vs. TrEMBL
Match: A0A0B0NLB8_GOSAR (Nucleolar 14 OS=Gossypium arboreum GN=F383_18443 PE=4 SV=1)

HSP 1 Score: 932.9 bits (2410), Expect = 2.8e-268
Identity = 517/961 (53.80%), Postives = 646/961 (67.22%), Query Frame = 1

Query: 1   MAKLSVRSSSNNDKKGKKKKKKSSGPKALTMKVSAPKANPFESIWSHRKFDVLGKKRKGE 60
           MAK S   +    K  KK  KKS  P A++MK+ + K NPFE+IWS RKFD+LGKKRKGE
Sbjct: 1   MAKQSTPGAKTKKKSNKKHHKKSD-PDAISMKLKSQKPNPFETIWSRRKFDILGKKRKGE 60

Query: 61  ERRIGLARSLAIEKRKKTLLKEYERSRKSTEFSDKRIGEWDEELGEFDKAILRSQRELKR 120
           ERRIGLARSLAI+KRKKTLLKEYE+S KS+ F DKRIGE ++++GEF+K ILRSQRE + 
Sbjct: 61  ERRIGLARSLAIQKRKKTLLKEYEQSTKSSVFVDKRIGEQNDDMGEFEKGILRSQRERQL 120

Query: 121 KLSKSSKFNLSDGEEDDYFGTQNLGALPANDDFEDEIIPDDDE--DEAAAA---ETNKGA 180
           KL K SKFNLSDGEED+ F   + G+LP  DDFEDE++ DDD   DE  +      N  +
Sbjct: 121 KLRKRSKFNLSDGEEDE-FDAPDFGSLPERDDFEDEMLSDDDNYADEKRSTVLKHLNSHS 180

Query: 181 YRDTQQKGRLLEGEDAVRLLAVYSFPFYFIKRKSKKEVMEEVIAKSKFFKAQKAKDKEEN 240
            +D  + G L+EGE+               K KSKKE+MEEVI KSKFFKAQKA+DKEEN
Sbjct: 181 AKDPLE-GDLIEGEEN--------------KHKSKKEIMEEVILKSKFFKAQKARDKEEN 240

Query: 241 EQLVEELDKKFETLVQSEALLSLTGSGNSNALKALVQKSVSNEHLKKDNLPAAGKTETFN 300
           EQL++ELDK F +LVQS+ALLSLT  G  NALKALV KS+ +EH+KK+ L    K  T N
Sbjct: 241 EQLMDELDKSFSSLVQSQALLSLTEPGKMNALKALVNKSIPDEHVKKEELAVTQKAVTNN 300

Query: 301 Q-------------------------------------------EERQKRMLAPDNSSDE 360
           Q                                           EERQKRMLA D SSDE
Sbjct: 301 QEQPDSYDKLVHEMVLDMRARPSDRTKTPEEIAQEERERLERLEEERQKRMLATDYSSDE 360

Query: 361 EDDDAETAFVGKQNYISGDDLGDSFTLDDERNHKKGWVDDILRRKDADGTESEGDDSAED 420
           + ++AE  +  +   ISGDDLGDSF LDDE  +KKGWVD+IL RKDA  +E + +D +ED
Sbjct: 361 DGENAEKDYAQRPRAISGDDLGDSFALDDEPGNKKGWVDEILERKDAIDSEDDEEDDSED 420

Query: 421 SDDSQDDEDGDSDDESEEDDSNHGVKQ-SLKDWEQSDDDILDTESEDDDEASEGGKQ--Q 480
              ++D ++ +  +E EEDD N   K  SLKDWEQSDDD + T+ E+D+E  E  +    
Sbjct: 421 LGSAEDTDEDEESEEEEEDDENESEKTLSLKDWEQSDDDNVGTDLEEDEETDEHDEAIGD 480

Query: 481 DEVHPKGK--------------VDHEGPKKAHKRSIAKSK-----SFDQFLSLLANCS-- 540
           ++V  K +              VD + PK + K +  K          + L  L++    
Sbjct: 481 EDVDKKSRNKTNKTELKKCVESVDAKKPKASGKHTSTKLDIPFIIEAPKNLEELSSLLEN 540

Query: 541 --NSDIILIIGRIRASNAIQLTDKNLEKMQRFYGILLQYFAVSANKKPLNVELLNLLFKP 600
             N D+I+II RIRASNAI+L  +N +KMQ FYG+LLQYFAV ANKKPLN EL N L KP
Sbjct: 541 RSNDDVIVIINRIRASNAIKLAAENRKKMQVFYGVLLQYFAVLANKKPLNFELSNKLVKP 600

Query: 601 LMEMSMEIPFYAATCARMRISHTHQHFCVQNKSPENSLWPSSKTLILLRLWTMIFPCSDY 660
           +MEMS EIPF++A CAR RI  T   FC   K+ EN  WP+ KTL LLRLW+MIFPCSDY
Sbjct: 601 IMEMSTEIPFFSAICARERILRTRVQFCEALKNHENGCWPTLKTLFLLRLWSMIFPCSDY 660

Query: 661 NHVVITPTILLMCEYLMRCPILTCRDIAIGAFLCSLLLSVARQSSKFCPEAINFLRTLLA 720
            HVV TP +LLMCEYLMR PI++ RD+AIG+FLCS++L   +QS KFCPEAI FLRTLL 
Sbjct: 661 RHVVTTPALLLMCEYLMRRPIMSGRDVAIGSFLCSMILMFMKQSRKFCPEAIMFLRTLLM 720

Query: 721 AAVSSS-SSSQNPQICHLVDSQALGKLLHIQNPTNEITPLDFFFIMNLNEDSLVFSSDNF 780
           AA     +S Q+ Q  H ++ +AL  LL I +  +EI PL+F  +M +++DS  F SDNF
Sbjct: 721 AATEHKLASEQDSQFYHFMELKALRPLLCIHDGVDEINPLNFLMVMEMSDDSSFFRSDNF 780

Query: 781 RAGLLSTVTETLDGFVNIYGQLKSFPEIFLPISTILHELAQQENMPDVLQNKFRKVAEAI 840
           RA  L TV ETL GF+ IY  L SFPEIFLPI+T+L E+++Q++MP  L++KF  V++ I
Sbjct: 781 RASALLTVIETLQGFIEIYDGLNSFPEIFLPIATLLVEVSEQKHMPKALKDKFNNVSQLI 840

Query: 841 EAKTEEHYMGRQPLRMRKQKTAPIKLLNPKFEENFVRGRDYDPDRERAERRKMQKLLKRE 887
           + K +E +  R+PL++RKQK APIKLLNPKFEENFV+GRDYDPDRERAERRK+QKL+KRE
Sbjct: 841 KKKADETHTLRRPLQLRKQKPAPIKLLNPKFEENFVKGRDYDPDRERAERRKLQKLIKRE 900

BLAST of Cucsa.327990 vs. TAIR10
Match: AT1G69070.1 (AT1G69070.1 FUNCTIONS IN: molecular_function unknown)

HSP 1 Score: 785.8 bits (2028), Expect = 2.8e-227
Identity = 441/919 (47.99%), Postives = 602/919 (65.51%), Query Frame = 1

Query: 14  KKGKKKKKKSSGPKALTMKVSAPKA-NPFESIWSHRKFDVLGKKRKGEERRIGLARSLAI 73
           KK  KK ++  GP A+ MK    K  NPFESI S RKFD+LGKKRKGEER + ++R+ A+
Sbjct: 9   KKPNKKGERRMGPDAVAMKAKTQKVDNPFESIRSRRKFDILGKKRKGEERFVSVSRTRAV 68

Query: 74  EKRKKTLLKEYERSRKSTEFSDKRIGEWDEELGEFDKAILRSQRELKRKLSKSSKFNLSD 133
           +KRK TL KEYE+S KS+ F DKRIGE ++ELGEFDK I+RSQR+ + KL+K S +NLSD
Sbjct: 69  DKRKNTLEKEYEQSLKSSVFLDKRIGEQNDELGEFDKGIIRSQRQRQLKLAKKSMYNLSD 128

Query: 134 GEEDDYFGTQNLGALPANDDFEDEIIPD----DDEDEAAAAETNKGAYRDTQQKGRLLEG 193
           GEED Y     LG     DDF+  ++ D    DD+ EA+A++  K   R+ +       G
Sbjct: 129 GEEDVYEDGA-LGGSSVKDDFDSGLLSDEDLQDDDLEASASKRLKHLNRNREVDA---SG 188

Query: 194 EDAVRLLAVYSFPFYFIKRKSKKEVMEEVIAKSKFFKAQKAKDKEENEQLVEELDKKFET 253
           E+               +RKSKKEVMEE+I KSK  + +KAK KEE  +L++ELDK F++
Sbjct: 189 EEE--------------RRKSKKEVMEEIIMKSKLGRMEKAKQKEEKGKLMDELDKNFKS 248

Query: 254 LVQSEALLSLT----GSGNSNALKALVQKSVS-------NEHLKKDNLPAAGKTETFN-- 313
           LV SEA+ SLT       N+     L    +S       +E  K     A  + E     
Sbjct: 249 LVNSEAMESLTKPFVAEENTRDPYLLSLNDMSMEIRARPSERTKTPEEIAQKEREKLEAL 308

Query: 314 QEERQKRMLAPDNSSDEEDDDAETAFVGKQNYISGDDLGDSFTLDDERNHKKGWVDDILR 373
           +EER+KRM   +  SD +++        +   ISGDDLGDSF++++++  K+GW+DD+L 
Sbjct: 309 EEERKKRMQETEELSDGDEEIGGEESTKRLTVISGDDLGDSFSVEEDKP-KRGWIDDVLE 368

Query: 374 RKD-ADGTESEGDDSAEDSDDSQDDEDGDSDDESEEDDSNHGVKQSLKDWEQSDDDI--- 433
           R+D  D +ES+ D+ +E  ++  +D+DG+SD   E+    H     L+DWEQSDD++   
Sbjct: 369 REDNVDNSESDEDEDSESEEE--EDDDGESDGGDEKQRKGH----HLEDWEQSDDELGAE 428

Query: 434 LDTESEDDDEASEGGKQQD-EVHPKGKVDHEGPKKAHKRS-------------------- 493
           L+ E EDDDE  +  +  +  VH K K D+  P K    S                    
Sbjct: 429 LEDEEEDDDEEDDDEEDAELRVHKKLKNDYAAPYKGEGLSGTVKEKTNMKKMSSTQRDIP 488

Query: 494 --IAKSKSFDQFLSLLANCSNSDIILIIGRIRASNAIQLTDKNLEKMQRFYGILLQYFAV 553
             I   K+F++ L+L+ +CSN D+ILI+ RIR +++I++  +N +KMQ FYG+LLQYFAV
Sbjct: 489 FMIDPPKNFEELLALVEDCSNEDVILIVNRIRIAHSIKIKAENRKKMQVFYGVLLQYFAV 548

Query: 554 SANKKPLNVELLNLLFKPLMEMSMEIPFYAATCARMRISHTHQHFCVQNKSPENSLWPSS 613
            A+KKPLN +LLN+L KPL+EMSMEIP++AA CAR R+  T   FC   K+PE+  WPS 
Sbjct: 549 LASKKPLNFDLLNMLVKPLIEMSMEIPYFAAICARQRLLKTRSQFCEAIKNPEDGCWPSL 608

Query: 614 KTLILLRLWTMIFPCSDYNHVVITPTILLMCEYLMRCPILTCRDIAIGAFLCSLLLSVAR 673
           KTL LLRLW++IFPCSD+ H V+TP+ILLMCEYLMRCPI + RDIAIG+FLCS++L    
Sbjct: 609 KTLFLLRLWSLIFPCSDFRHAVMTPSILLMCEYLMRCPISSGRDIAIGSFLCSIVLL--- 668

Query: 674 QSSKFCPEAINFLRTLLAAAVSSSS-SSQNPQICHLVDSQALGKLLHIQNPTNEITPLDF 733
           QS KFCPEAI F+RTLL AA    S +S   +  H ++ ++L  LL IQ+   E+ PL+F
Sbjct: 669 QSKKFCPEAILFIRTLLMAASDKKSPASAESEFYHFMELKSLTPLLCIQDNVKEVMPLNF 728

Query: 734 FFIMNLNEDSLVFSSDNFRAGLLSTVTETLDGFVNIYGQLKSFPEIFLPISTILHELAQQ 793
             IMN   DS  FSSD+FRA +LS+V ETL+GFV I G L SFPEIF+PIST+LH++  Q
Sbjct: 729 LKIMNEPADSPYFSSDDFRASILSSVVETLEGFVEINGGLSSFPEIFMPISTLLHQIGNQ 788

Query: 794 ENMPDVLQNKFRKVAEAIEAKTEEHYMGRQPLRMRKQKTAPIKLLNPKFEENFVRGRDYD 853
           E +P  L+ K   VA+ IE KT++H+  R+PL MRK K   I+++NPKFEENFV G D D
Sbjct: 789 EKIPQTLKEKLEDVAKLIEKKTDDHHKERKPLSMRKHKPVAIRMVNPKFEENFVPGMDND 848

Query: 854 PDRERAERRKMQKLLKRETKGAARELRKDNHFLSEVKARDKAKQDEERAEKYKKARTFLE 887
           PD+ R++ +K+++ LKRE +GA RELRKD++F+S VKA++KA  ++ERAEK+ KA  FL+
Sbjct: 849 PDKYRSDLKKLKRKLKREARGAVRELRKDSYFMSTVKAKEKAAHEQERAEKHGKAWAFLQ 899

BLAST of Cucsa.327990 vs. TAIR10
Match: AT5G63740.1 (AT5G63740.1 RING/U-box superfamily protein)

HSP 1 Score: 58.5 bits (140), Expect = 2.4e-08
Identity = 37/139 (26.62%), Postives = 60/139 (43.17%), Query Frame = 1

Query: 297 EERQKRMLAPDNSSDEEDDDAETAFVGKQNYISGDDLGDSFTLDDER-----NHKKGWVD 356
           E  Q+     D   +E +    T  +     +S    G   T +DE      +H      
Sbjct: 2   ENDQQGSYLRDEDKEEMEKRIRTVVIDSNLVLSNSTCGICRTAEDEYIKVYDDHNNDGEG 61

Query: 357 DILRRKDADGTESEGDDSAEDSDDSQDDEDGDSDDESEEDDSNHGVKQSLKDWEQSDDDI 416
           D     D D  E E  D+ ED D+ +D++D D DD+ ++DD++        D ++ DDD 
Sbjct: 62  DGDGDGDGDEDEDEDADADEDEDEDEDEDDDDDDDDDDDDDADDA------DDDEDDDDE 121

Query: 417 LDTESEDDDEASEGGKQQD 431
            D E EDDD+  +    ++
Sbjct: 122 DDDEDEDDDDDDDDENDEE 134

BLAST of Cucsa.327990 vs. TAIR10
Match: AT5G55660.1 (AT5G55660.1 DEK domain-containing chromatin associated protein)

HSP 1 Score: 56.2 bits (134), Expect = 1.2e-07
Identity = 67/311 (21.54%), Postives = 128/311 (41.16%), Query Frame = 1

Query: 135 EDDYFGTQNLGALPANDDFEDEIIPDDDEDEAAAAETNKGAYRDTQQKGRLLEGEDAVRL 194
           E D  G Q+ G     D  E E+  ++ +D     E  +    D     + L+ +D V  
Sbjct: 56  EIDDEGKQHEGESETGDK-EVEVTEEEKKDVGEDKEQPEADKMDEDTDDKNLKADDGVSG 115

Query: 195 LAVYSFPFYFIKRKSKKEVMEEVIAKSKFFKAQKAKDKEENEQLVEELDKKFETLVQSEA 254
           +A             +  VM+E +  +    A+  + ++E E   E+L+           
Sbjct: 116 VAT-----------EEDAVMKESVESADNKDAENPEGEQEKESKEEKLEG---------- 175

Query: 255 LLSLTGSGNSNALKALVQKSVSNEHLKKDNLPAAGKTETFNQEERQKRMLAPDNSSDEED 314
                G  N N      +K V  +  K D++  A K E  +++++++ +   + +   E+
Sbjct: 176 -----GKANGNEEGDTEEKLVGGD--KGDDVDEAEKVENVDEDDKEEALKEKNEAELAEE 235

Query: 315 DDAETAFVGKQNYISGDDLGDSFTLDDERNHKKGWVDDILRRKDADGTESEGDDSAEDSD 374
           ++       K+     D   D+   + E   KK    D     + +  E E +D  E+S 
Sbjct: 236 EETNKGEEVKEANKEDDVEADTKVAEPEVEDKKTESKD-----ENEDKEEEKEDEKEESM 295

Query: 375 DSQDDEDGDSDDESEEDDSNHGVKQSLKDWEQSDDDILDTESEDDDEASEGGKQQDEVHP 434
           D ++DE  +S+D+ +ED+            E+S+DD  D + ED  ++++ GK + E   
Sbjct: 296 DDKEDEKEESNDDDKEDEK-----------EESNDDKED-KKEDIKKSNKRGKGKTE-KT 319

Query: 435 KGKVDHEGPKK 446
           +GK   +  KK
Sbjct: 356 RGKTKSDEEKK 319

BLAST of Cucsa.327990 vs. TAIR10
Match: AT1G44050.1 (AT1G44050.1 Cysteine/Histidine-rich C1 domain family protein)

HSP 1 Score: 53.9 bits (128), Expect = 5.8e-07
Identity = 24/72 (33.33%), Postives = 37/72 (51.39%), Query Frame = 1

Query: 360 DGTESEGDDSAEDSDDSQDDEDGDSDDESEEDDSNHGVKQSLKDWEQSDDDILDTESEDD 419
           D  ++E DD+ +D +D  DD  GD  D+  EDD+         D +  DDD  D + +D 
Sbjct: 71  DDDDAEDDDNGDDKEDGDDDNKGDDGDDDNEDDNEDDDNDDDDDDDDDDDDDDDDDDDDG 130

Query: 420 DEASEGGKQQDE 432
           D+ +E G   D+
Sbjct: 131 DDDNEDGDCDDD 142

HSP 2 Score: 52.8 bits (125), Expect = 1.3e-06
Identity = 39/137 (28.47%), Postives = 58/137 (42.34%), Query Frame = 1

Query: 295 NQEERQKRMLAPDNSSDEEDDDAETAFVGKQNYISGDDLGDSFTLDDERNHKKGWVDDIL 354
           N  + + +M   D+  D EDDD            +GDD  D    DD+     G  D+  
Sbjct: 58  NFHKLRLKMHNHDDDDDAEDDD------------NGDDKEDG---DDDNKGDDGDDDNED 117

Query: 355 RRKDADGTESEGDDSAEDSDDSQDDEDGDSDDESEEDDSNHGVKQSLKDWEQSDDDILDT 414
             +D D  + + DD  +D DD  DD+DGD D+E  + D + G            D +L  
Sbjct: 118 DNEDDDNDDDDDDDDDDDDDDDDDDDDGDDDNEDGDCDDDDG------------DLLLPF 167

Query: 415 ESEDDDEASEGGKQQDE 432
           +S     ++  G QQ E
Sbjct: 178 DSTPHFPSTRSGDQQGE 167

HSP 3 Score: 50.1 bits (118), Expect = 8.4e-06
Identity = 30/115 (26.09%), Postives = 51/115 (44.35%), Query Frame = 1

Query: 358 DADGTESEGDDSAEDSDDSQDDEDGDSDDESEEDDSNHGVKQSLKDWEQSDDDILDTESE 417
           D D  + +  D  ED DD    +DGD D+E + +D ++       D +  DDD  D + +
Sbjct: 72  DDDAEDDDNGDDKEDGDDDNKGDDGDDDNEDDNEDDDN------DDDDDDDDDDDDDDDD 131

Query: 418 DDDEASEGGKQQDEVHPKGKVDHEGPKKAHKRSIAKSKSFDQFLSLLANCSNSDI 473
           DDD+  +  +  D     G +        H      ++S DQ    L +C++ D+
Sbjct: 132 DDDDGDDDNEDGDCDDDDGDLLLPFDSTPH---FPSTRSGDQQGESLLDCNHPDV 177

BLAST of Cucsa.327990 vs. TAIR10
Match: AT5G15140.1 (AT5G15140.1 Galactose mutarotase-like superfamily protein)

HSP 1 Score: 52.8 bits (125), Expect = 1.3e-06
Identity = 36/129 (27.91%), Postives = 61/129 (47.29%), Query Frame = 1

Query: 283 DNLPAAGKTETFNQEERQKR---MLAPDNSSDEEDDDAETAFVGKQNYISGDDLGDSFTL 342
           + +  AG  +  N ++ +K+   +   D   + +D D E   V K+    G D  D    
Sbjct: 56  EKVDGAGSDDEDNDKKEKKKEHDVQKKDKQHENKDKDDEKKHVDKKKS-GGHDKDD---- 115

Query: 343 DDERNHKKGWVDDILRRKDADGTESEGDDSAEDSDDSQDDEDGDSDDESEEDDSNHGVKQ 402
           DDE+ HK        ++KD     ++ DDS +D+DD  DD+D D DD+  + D N   K 
Sbjct: 116 DDEKKHKD-------KKKDG---HNDDDDSDDDTDDDDDDDDDDDDDDEVDGDDNEKEKI 169

Query: 403 SLKDWEQSD 409
            L + ++ +
Sbjct: 176 GLYELKKGN 169

HSP 2 Score: 41.6 bits (96), Expect = 3.0e-03
Identity = 30/104 (28.85%), Postives = 45/104 (43.27%), Query Frame = 1

Query: 330 GDDLGDSFTLDDERNHKKGWVDDI-LRRKDADGTESEGDDSAEDSDDSQDD-EDGDSDDE 389
           G +  D    DDE N KK    +  +++KD      + DD  +  D  +    D D DDE
Sbjct: 54  GGEKVDGAGSDDEDNDKKEKKKEHDVQKKDKQHENKDKDDEKKHVDKKKSGGHDKDDDDE 113

Query: 390 SEEDDSNHGVKQSLKDWEQSDDDILDTESEDDDEASEGGKQQDE 432
            +  D     K    D + SDDD  D + +DDD+  +     D+
Sbjct: 114 KKHKDKK---KDGHNDDDDSDDDTDDDDDDDDDDDDDDEVDGDD 154

BLAST of Cucsa.327990 vs. NCBI nr
Match: gi|743798096|ref|XP_011009770.1| (PREDICTED: nucleolar protein 14 isoform X4 [Populus euphratica])

HSP 1 Score: 986.5 bits (2549), Expect = 3.1e-284
Identity = 542/970 (55.88%), Postives = 664/970 (68.45%), Query Frame = 1

Query: 1   MAKLSVRSSSNNDKKGKKKKKKS-SGPKALTMKVSAPK------ANPFESIWSHRKFDVL 60
           MAK S RS S+++ K KKKKK S + PK++ MK SA        +NPFE+IWS RKFD+L
Sbjct: 1   MAKTSKRSRSSSNTKSKKKKKNSRTAPKSVAMKASAASNDNKNSSNPFETIWSRRKFDIL 60

Query: 61  GKKRKGEERRIGLARSLAIEKRKKTLLKEYERSRKSTEFSDKRIGEWDEELGEFDKAILR 120
           GKKRKGEE RIGL+R  AIEKRKKTLLKEYE S KS+ F DKRIGE +E+LGEFDKAI+R
Sbjct: 61  GKKRKGEELRIGLSRCRAIEKRKKTLLKEYEESGKSSVFLDKRIGEQNEQLGEFDKAIIR 120

Query: 121 SQRELKRKLSKSSKFNLSDGEEDDYFGTQNLGALPANDDFEDEIIPDDDEDEAAAAET-N 180
           SQRE + K +K SK+NLSDGEEDD FG  NLG L   DDFEDEI  DDD D+A A  T N
Sbjct: 121 SQRERQLK-NKKSKYNLSDGEEDDDFGIPNLGPLSGLDDFEDEIFSDDDGDDADADGTSN 180

Query: 181 KGAYRDTQQKGRL----LEGEDAVRLLAVYSFPFYFIKRKSKKEVMEEVIAKSKFFKAQK 240
           K A         L    ++GE+               K K+KKEVM+EVI KSKFFKAQK
Sbjct: 181 KPAILKQLNAHGLPQDAVDGEEN--------------KPKTKKEVMQEVILKSKFFKAQK 240

Query: 241 AKDKEENEQLVEELDKKFETLVQSEALLSLTGSGNSNALKALVQKSVSNEHLKKDNLPAA 300
           AKDKEENEQL+EELDK F +LVQS+AL SLT  G  NALKALV K + NEH+KKD LP  
Sbjct: 241 AKDKEENEQLMEELDKSFTSLVQSQALSSLTEPGKMNALKALVNKDIPNEHVKKDELPVI 300

Query: 301 GKTETFNQE-------------------------------------------ERQKRMLA 360
            K ETF QE                                           +R+KRML 
Sbjct: 301 QKPETFKQEQPDSYDKLVYEMAIDSRARPSDRTKTPEEIAQKERERLEQLEKDRKKRMLV 360

Query: 361 PDNSSDEEDDDAETAFVGKQNYISGDDLGDSFTLDDERNHKKGWVDDILRRKDADGTESE 420
            D+SSDEE+DD E     +   ISGDDLGDSF+L +E    KGWVD+IL RK+A+ +++E
Sbjct: 361 ADDSSDEENDDVEKLSAQRPRSISGDDLGDSFSLYEEPGTAKGWVDEILARKEANDSDNE 420

Query: 421 GDDSAEDSDDSQDD-EDGDSDDESEEDDSNHGVKQSLKDWEQSDDDILDTESEDDDEASE 480
            DDS+E+S  + DD +D  SD++++ DD  H    SLKDWEQSDDD L T+ E+D+E   
Sbjct: 421 DDDSSEESVSANDDGDDEGSDEDADGDDDEHEKSTSLKDWEQSDDDNLGTDLEEDEEHGS 480

Query: 481 GGKQQDEVHP-----KGKVDHEGPKKAHKRS----------------------IAKSKSF 540
                 E+ P       K +   P+K  ++S                      I   KSF
Sbjct: 481 HDDDDGEIEPISHKKSKKTEPAEPRKGDEKSLDGKKKKANREQHSTQPDIPHIIEAPKSF 540

Query: 541 DQFLSLLANCSNSDIILIIGRIRASNAIQLTDKNLEKMQRFYGILLQYFAVSANKKPLNV 600
           ++F ++L NCSN ++IL++ RIR SNAIQL  +N +K+Q FYG+LLQYFAV ANKKPLN+
Sbjct: 541 EEFCAILENCSNENVILVVDRIRKSNAIQLAAENRKKIQVFYGVLLQYFAVLANKKPLNI 600

Query: 601 ELLNLLFKPLMEMSMEIPFYAATCARMRISHTHQHFCVQNKSPENSLWPSSKTLILLRLW 660
           ELLNLL KPLMEMS+EIP+++A CAR RI  T   FC   K+ ENS WPS KTL LLRLW
Sbjct: 601 ELLNLLVKPLMEMSVEIPYFSAICARQRILRTRAQFCEALKNTENSCWPSMKTLSLLRLW 660

Query: 661 TMIFPCSDYNHVVITPTILLMCEYLMRCPILTCRDIAIGAFLCSLLLSVARQSSKFCPEA 720
           +MIFPCSD+ HVV+TP ILLM EYLMRCPIL+ RDIAIG+FLC+++LS+ +QS KFCPEA
Sbjct: 661 SMIFPCSDFRHVVMTPVILLMSEYLMRCPILSGRDIAIGSFLCTMVLSITKQSQKFCPEA 720

Query: 721 INFLRTLLAAAVSSSSSS-QNPQICHLVDSQALGKLLHIQNPTNEITPLDFFFIMNLNED 780
           I FLRTLL A      SS Q  Q  HL++ + L  LLHI +  NEI PL+F  +M++ ED
Sbjct: 721 IMFLRTLLMATTERKPSSYQESQFYHLMELKELKPLLHIHDHVNEIRPLNFLMVMDMKED 780

Query: 781 SLVFSSDNFRAGLLSTVTETLDGFVNIYGQLKSFPEIFLPISTILHELAQQENMPDVLQN 840
           +  FSSD+FR G+L T+ ETL GFV+IY +L SFPEIFLPIS +L E+AQQENMP  LQ+
Sbjct: 781 ASFFSSDDFRVGVLVTMVETLQGFVDIYKELSSFPEIFLPISMLLLEVAQQENMPATLQD 840

Query: 841 KFRKVAEAIEAKTEEHYMGRQPLRMRKQKTAPIKLLNPKFEENFVRGRDYDPDRERAERR 887
           KF+ VAE I  K  +H+M R+PL+M+K+K  PIKL+ PKFEENFV+GRDYDPDRERAERR
Sbjct: 841 KFKDVAELINKKANKHHMMRKPLQMQKKKPVPIKLVAPKFEENFVKGRDYDPDRERAERR 900

BLAST of Cucsa.327990 vs. NCBI nr
Match: gi|743798092|ref|XP_011009760.1| (PREDICTED: nucleolar protein 14 isoform X3 [Populus euphratica])

HSP 1 Score: 981.9 bits (2537), Expect = 7.5e-283
Identity = 542/971 (55.82%), Postives = 664/971 (68.38%), Query Frame = 1

Query: 1   MAKLSVRSSSNNDKKGKKKKKKS-SGPKALTMKVSAPK------ANPFESIWSHRKFDVL 60
           MAK S RS S+++ K KKKKK S + PK++ MK SA        +NPFE+IWS RKFD+L
Sbjct: 1   MAKTSKRSRSSSNTKSKKKKKNSRTAPKSVAMKASAASNDNKNSSNPFETIWSRRKFDIL 60

Query: 61  GKKRKGEERRIGLARSLAIEKRKKTLLKEYERSRKSTEFSDKRIGEWDEELGEFDKAILR 120
           GKKRKGEE RIGL+R  AIEKRKKTLLKEYE S KS+ F DKRIGE +E+LGEFDKAI+R
Sbjct: 61  GKKRKGEELRIGLSRCRAIEKRKKTLLKEYEESGKSSVFLDKRIGEQNEQLGEFDKAIIR 120

Query: 121 SQRELKRKLSKSSKFNLSDGEEDDYFGTQNLGALPANDDFEDEIIPDDDEDEAAAAET-N 180
           SQRE + K +K SK+NLSDGEEDD FG  NLG L   DDFEDEI  DDD D+A A  T N
Sbjct: 121 SQRERQLK-NKKSKYNLSDGEEDDDFGIPNLGPLSGLDDFEDEIFSDDDGDDADADGTSN 180

Query: 181 KGAYRDTQQKGRL----LEGEDAVRLLAVYSFPFYFIKRKSKKEVMEEVIAKSKFFKAQK 240
           K A         L    ++GE+               K K+KKEVM+EVI KSKFFKAQK
Sbjct: 181 KPAILKQLNAHGLPQDAVDGEEN--------------KPKTKKEVMQEVILKSKFFKAQK 240

Query: 241 AKDKEENEQLVEELDKKFETLVQSEALLSLTGSGNSNALKALVQKSVSNEHLKKDNLPAA 300
           AKDKEENEQL+EELDK F +LVQS+AL SLT  G  NALKALV K + NEH+KKD LP  
Sbjct: 241 AKDKEENEQLMEELDKSFTSLVQSQALSSLTEPGKMNALKALVNKDIPNEHVKKDELPVI 300

Query: 301 GKTETFNQE-------------------------------------------ERQKRMLA 360
            K ETF QE                                           +R+KRML 
Sbjct: 301 QKPETFKQEQPDSYDKLVYEMAIDSRARPSDRTKTPEEIAQKERERLEQLEKDRKKRMLV 360

Query: 361 PDNSSDEEDDDAETAFVGKQNYISGDDLGDSFTLDDERNHKKGWVDDILRRKDADGTESE 420
            D+SSDEE+DD E     +   ISGDDLGDSF+L +E    KGWVD+IL RK+A+ +++E
Sbjct: 361 ADDSSDEENDDVEKLSAQRPRSISGDDLGDSFSLYEEPGTAKGWVDEILARKEANDSDNE 420

Query: 421 GDDSAEDSDDSQDD-EDGDSDDESEEDDSNHGVKQSLKDWEQSDDDILDTESEDDDEASE 480
            DDS+E+S  + DD +D  SD++++ DD  H    SLKDWEQSDDD L T+ E+D+E   
Sbjct: 421 DDDSSEESVSANDDGDDEGSDEDADGDDDEHEKSTSLKDWEQSDDDNLGTDLEEDEEHGS 480

Query: 481 GGKQQDEVHP-----KGKVDHEGPKKAHKRS----------------------IAKSKSF 540
                 E+ P       K +   P+K  ++S                      I   KSF
Sbjct: 481 HDDDDGEIEPISHKKSKKTEPAEPRKGDEKSLDGKKKKANREQHSTQPDIPHIIEAPKSF 540

Query: 541 DQFLSLLANCSNSDIILIIGRIRASNAIQLTDKNLEKMQRFYGILLQYFAVSANKKPLNV 600
           ++F ++L NCSN ++IL++ RIR SNAIQL  +N +K+Q FYG+LLQYFAV ANKKPLN+
Sbjct: 541 EEFCAILENCSNENVILVVDRIRKSNAIQLAAENRKKIQVFYGVLLQYFAVLANKKPLNI 600

Query: 601 ELLNLLFKPLMEMSMEIPFYAATCARMRISHTHQHFCVQNK-SPENSLWPSSKTLILLRL 660
           ELLNLL KPLMEMS+EIP+++A CAR RI  T   FC   K + ENS WPS KTL LLRL
Sbjct: 601 ELLNLLVKPLMEMSVEIPYFSAICARQRILRTRAQFCEALKNTAENSCWPSMKTLSLLRL 660

Query: 661 WTMIFPCSDYNHVVITPTILLMCEYLMRCPILTCRDIAIGAFLCSLLLSVARQSSKFCPE 720
           W+MIFPCSD+ HVV+TP ILLM EYLMRCPIL+ RDIAIG+FLC+++LS+ +QS KFCPE
Sbjct: 661 WSMIFPCSDFRHVVMTPVILLMSEYLMRCPILSGRDIAIGSFLCTMVLSITKQSQKFCPE 720

Query: 721 AINFLRTLLAAAVSSSSSS-QNPQICHLVDSQALGKLLHIQNPTNEITPLDFFFIMNLNE 780
           AI FLRTLL A      SS Q  Q  HL++ + L  LLHI +  NEI PL+F  +M++ E
Sbjct: 721 AIMFLRTLLMATTERKPSSYQESQFYHLMELKELKPLLHIHDHVNEIRPLNFLMVMDMKE 780

Query: 781 DSLVFSSDNFRAGLLSTVTETLDGFVNIYGQLKSFPEIFLPISTILHELAQQENMPDVLQ 840
           D+  FSSD+FR G+L T+ ETL GFV+IY +L SFPEIFLPIS +L E+AQQENMP  LQ
Sbjct: 781 DASFFSSDDFRVGVLVTMVETLQGFVDIYKELSSFPEIFLPISMLLLEVAQQENMPATLQ 840

Query: 841 NKFRKVAEAIEAKTEEHYMGRQPLRMRKQKTAPIKLLNPKFEENFVRGRDYDPDRERAER 887
           +KF+ VAE I  K  +H+M R+PL+M+K+K  PIKL+ PKFEENFV+GRDYDPDRERAER
Sbjct: 841 DKFKDVAELINKKANKHHMMRKPLQMQKKKPVPIKLVAPKFEENFVKGRDYDPDRERAER 900

BLAST of Cucsa.327990 vs. NCBI nr
Match: gi|566190903|ref|XP_002316014.2| (hypothetical protein POPTR_0010s15000g [Populus trichocarpa])

HSP 1 Score: 974.2 bits (2517), Expect = 1.6e-280
Identity = 536/974 (55.03%), Postives = 658/974 (67.56%), Query Frame = 1

Query: 1   MAKLSVRS---SSNNDKKGKKKKKKSSGPKALTMKVSAPK------ANPFESIWSHRKFD 60
           MAK S RS   SS+N K  KKKK   + P ++ MK SA        +NPFE+IWS RKFD
Sbjct: 1   MAKTSKRSRSSSSSNTKSKKKKKNSRTAPNSVAMKASAASKDNKNSSNPFETIWSRRKFD 60

Query: 61  VLGKKRKGEERRIGLARSLAIEKRKKTLLKEYERSRKSTEFSDKRIGEWDEELGEFDKAI 120
           +LGKKRKGEE RIGL+R  AIEKRKKTLLKEYE S KS+ F DKRIGE +E+LGEFDKAI
Sbjct: 61  ILGKKRKGEELRIGLSRCRAIEKRKKTLLKEYEESGKSSVFLDKRIGEQNEQLGEFDKAI 120

Query: 121 LRSQRELKRKLSKSSKFNLSDGEEDDYFGTQNLGALPANDDFEDEIIPDDDEDEAAAAET 180
           +RSQRE + K +K SK+NLSDGEEDD FG  NLG L   DDFEDEI+ DDD D+A A  T
Sbjct: 121 IRSQRERQLK-NKKSKYNLSDGEEDDDFGIPNLGPLSGQDDFEDEILSDDDGDDADADRT 180

Query: 181 NKGAYRDTQQKGRLLEGEDAVRLLAVYSFPFYFI-----KRKSKKEVMEEVIAKSKFFKA 240
           +K        K  +L      R L  +  P   +     K K+KKEVM+EVI KSKFFKA
Sbjct: 181 SK--------KPAIL------RQLNAHGLPQDAVHGEENKPKTKKEVMQEVILKSKFFKA 240

Query: 241 QKAKDKEENEQLVEELDKKFETLVQSEALLSLTGSGNSNALKALVQKSVSNEHLKKDNLP 300
           QKAKDKEENEQL+EELDK F +LVQS+AL SLT  G  NALKALV K + NEH+KKD LP
Sbjct: 241 QKAKDKEENEQLMEELDKSFTSLVQSQALSSLTEPGKMNALKALVNKDIPNEHVKKDELP 300

Query: 301 AAGKTETFNQEER-------------------QKRMLAP--------------------- 360
              K ETF Q+E+                     R   P                     
Sbjct: 301 VIQKPETFKQQEQPDSYDKLVYEMAIDSRARPSDRTKTPEEIAQKERERLEQLEEDRKKR 360

Query: 361 ----DNSSDEEDDDAETAFVGKQNYISGDDLGDSFTLDDERNHKKGWVDDILRRKDADGT 420
               D+SSDEE+DD E     +   ISGDDLGDSF+L +E    KGWVD+IL RK+AD +
Sbjct: 361 MLVADDSSDEENDDVEKLSAQRPRSISGDDLGDSFSLYEEPGTTKGWVDEILARKEADDS 420

Query: 421 ESEGDDSAEDSDDSQD--DEDGDSDDESEEDDSNHGVKQSLKDWEQSDDDILDTESEDDD 480
           ++E DDS+E+S  + D  D++G  +D+++ DD  H    SLKDWEQSDDD L T+ E+D+
Sbjct: 421 DNEDDDSSEESASANDDGDDEGSDEDDTDGDDDEHEKSTSLKDWEQSDDDNLGTDLEEDE 480

Query: 481 EASEGGKQQDEVHP-----KGKVDHEGPKKAHKRS----------------------IAK 540
           E         E+ P       K +   P+K  ++S                      I  
Sbjct: 481 EHGSHDGDDGEIEPISHKKSKKTEPVEPRKGDEKSLDGKKKKANREQHSTQPDIPHIIEA 540

Query: 541 SKSFDQFLSLLANCSNSDIILIIGRIRASNAIQLTDKNLEKMQRFYGILLQYFAVSANKK 600
            KSF++F ++L NCSN ++IL++ RIR SNAIQL  +N +K+Q FYG+LLQYFAV ANKK
Sbjct: 541 PKSFEEFCAILENCSNENVILVVDRIRKSNAIQLAAENRKKIQVFYGVLLQYFAVLANKK 600

Query: 601 PLNVELLNLLFKPLMEMSMEIPFYAATCARMRISHTHQHFCVQNKSPENSLWPSSKTLIL 660
           PLN+ELLN L KPLMEMS+EIP+++A CAR RI  T   FC   K+ ENS WPS KTL L
Sbjct: 601 PLNIELLNFLVKPLMEMSVEIPYFSAICARQRILRTRAQFCEALKNTENSSWPSMKTLSL 660

Query: 661 LRLWTMIFPCSDYNHVVITPTILLMCEYLMRCPILTCRDIAIGAFLCSLLLSVARQSSKF 720
           LRLW+MIFPCSD+ HVV+TP ILLM EYLMRCPIL+ RDIAIG+FLC+++LS+ +QS KF
Sbjct: 661 LRLWSMIFPCSDFRHVVMTPVILLMSEYLMRCPILSGRDIAIGSFLCTMVLSITKQSQKF 720

Query: 721 CPEAINFLRTLLAAAVSSSSSS-QNPQICHLVDSQALGKLLHIQNPTNEITPLDFFFIMN 780
           CPEAI FLRTLL A      SS Q  Q  HL++ + +  LLHI +  NEI PL+F  +M+
Sbjct: 721 CPEAIMFLRTLLMATTERKPSSYQESQFYHLMELKEIKPLLHIHDHVNEIRPLNFLMVMD 780

Query: 781 LNEDSLVFSSDNFRAGLLSTVTETLDGFVNIYGQLKSFPEIFLPISTILHELAQQENMPD 840
           + ED+  FSSD+FR G+L T+ ETL GFV+IY +L SFPEIFLPIS +L E+AQQENMP 
Sbjct: 781 MQEDTSFFSSDDFRVGVLVTMVETLQGFVDIYKELSSFPEIFLPISMLLLEVAQQENMPA 840

Query: 841 VLQNKFRKVAEAIEAKTEEHYMGRQPLRMRKQKTAPIKLLNPKFEENFVRGRDYDPDRER 887
            LQ+KF+ VAE I  K  +H+M R+PL+M+K+K  PIKL+ PKFEENFV+GRDYDPDRER
Sbjct: 841 TLQDKFKDVAELINKKANKHHMMRKPLQMQKKKPVPIKLVAPKFEENFVKGRDYDPDRER 900

BLAST of Cucsa.327990 vs. NCBI nr
Match: gi|1000970163|ref|XP_015573689.1| (PREDICTED: nucleolar protein 14 [Ricinus communis])

HSP 1 Score: 962.2 bits (2486), Expect = 6.2e-277
Identity = 533/958 (55.64%), Postives = 664/958 (69.31%), Query Frame = 1

Query: 1   MAKLSVRSSSNNDKKGKKKKKKSSGPKALTMKV-----SAPKANPFESIWSHRKFDVLGK 60
           MAK + RS S  D K KKKK K SGP  ++MK+     +APK NPFE+IWS RKFD+LGK
Sbjct: 1   MAKTAKRSGS--DTKNKKKKSKKSGPNTVSMKLKTSTSTAPKHNPFETIWSRRKFDILGK 60

Query: 61  KR-KGEERRIGLARSLAIEKRKKTLLKEYERSRKSTEFSDKRIGEWDEELGEFDKAILRS 120
           K+ KGEERRIGLARSLAI+KRKKTLLKEYE+S KS+ F DKRIGE ++EL EFDKAI+RS
Sbjct: 61  KKGKGEERRIGLARSLAIDKRKKTLLKEYEQSGKSSVFVDKRIGEKNDELEEFDKAIMRS 120

Query: 121 QRELKRKLSKSSKFNLSDGEEDDYFGTQNLGALPANDDFEDEIIPDDDEDEAAAAETNKG 180
           QRE + KLSK SK+NLSDGEE+D F   NLG L   DDF++ ++ DDD D      T K 
Sbjct: 121 QRERQMKLSKKSKYNLSDGEEED-FEIPNLGPLSERDDFDEGMLSDDDNDAPYGTTTLKQ 180

Query: 181 A-YRDTQ--QKGRLLEGEDAVRLLAVYSFPFYFIKRKSKKEVMEEVIAKSKFFKAQKAKD 240
               DT   ++   LEGE+               K K+KKEVMEEVI KSKFFKAQKAKD
Sbjct: 181 LDAHDTPNLREQGALEGEEK--------------KHKTKKEVMEEVILKSKFFKAQKAKD 240

Query: 241 KEENEQLVEELDKKFETLVQSEALLSLTGSGNSNALKALVQKSVSNEHLKKDNLPAAGKT 300
           KEENEQL+E+LDK F +LVQS  LLSLT  G  NALKALV K + +  L      A G+ 
Sbjct: 241 KEENEQLMEDLDKSFTSLVQSRVLLSLTEPGKMNALKALVNKDIPDGLLSTQKPEAIGQD 300

Query: 301 ETFN-------------------------------------QEERQKRMLAPDNSSDEED 360
           +  +                                     +EER+KRMLA +NSSDEE+
Sbjct: 301 QPDSYDKLVKGMILDMRAHPSDRTKTPEEIAQEEREQLERLEEERRKRMLATNNSSDEEN 360

Query: 361 DDAETAFVGKQNYISGDDLGDSFTLDDERNHKKGWVDDILRRKDADGTESEGDDSAEDSD 420
           DD E   +     +SGDDLGDSF+L +E   KKGWVD+IL R+D + +E+E  + +EDS+
Sbjct: 361 DDVEKQSMQSIRSVSGDDLGDSFSLQEEPKAKKGWVDEILERRDVEDSENEDLNLSEDSE 420

Query: 421 DSQDDEDGDSDDESEEDDSN--HGVKQSLKDWEQSDDDILDTESEDDDEASEGGKQQDEV 480
            ++DD D +  D+S+  + N  +    SLKDWEQSDDD L T+ E D+E        +E+
Sbjct: 421 RAEDDGDSEGSDDSDSGEHNDENDKPLSLKDWEQSDDDNLGTDLEGDEEYDNLDDGNEEI 480

Query: 481 HPKGKV------DHEGPK-----------KAHKRS------IAKSKSFDQFLSLLANCSN 540
            P+G+       D E  K           K H         I   KSF++  +LL NCSN
Sbjct: 481 EPRGQKKSKKNDDVESRKGDGVSLVTKKTKQHSTEPDIPFLIEAPKSFEELCALLDNCSN 540

Query: 541 SDIILIIGRIRASNAIQLTDKNLEKMQRFYGILLQYFAVSANKKPLNVELLNLLFKPLME 600
           ++++++I RIRASNAI+L  +N +KMQ FYG+LLQYFAV ANKKPLN ELLNLL KPL+E
Sbjct: 541 ANVMVVINRIRASNAIKLAAENRKKMQVFYGVLLQYFAVLANKKPLNFELLNLLVKPLIE 600

Query: 601 MSMEIPFYAATCARMRISHTHQHFCVQNKSPENSLWPSSKTLILLRLWTMIFPCSDYNHV 660
           MSMEIP+++A CAR RI  T   FC   K+ E+  WPS KTL LLRLW+M+FPCSD+ HV
Sbjct: 601 MSMEIPYFSAICARQRILRTRAQFCESIKNRESGCWPSMKTLSLLRLWSMVFPCSDFRHV 660

Query: 661 VITPTILLMCEYLMRCPILTCRDIAIGAFLCSLLLSVARQSSKFCPEAINFLRTLLAAAV 720
           V+TP ILLMCEYLMRCPI + RDIA+G+FLCS+LLSV +QS KFCPEAI FL+TLL AAV
Sbjct: 661 VMTPAILLMCEYLMRCPIFSGRDIAVGSFLCSILLSVTKQSKKFCPEAIVFLQTLLKAAV 720

Query: 721 SSSSSS-QNPQICHLVDSQALGKLLHIQNPTNEITPLDFFFIMNLNEDSLVFSSDNFRAG 780
              S+S +  QI HLV+ ++LG LL +++  NEI PL+FF IM++ EDS  FSSDNFRA 
Sbjct: 721 EQKSASYRESQIYHLVELKSLGSLLFMRHCVNEINPLNFFMIMDMPEDSSFFSSDNFRAS 780

Query: 781 LLSTVTETLDGFVNIYGQLKSFPEIFLPISTILHELAQQENMPDVLQNKFRKVAEAIEAK 840
           +L T  ETL G+V+IY  L SFPEIFLPIST+L E+A+Q+N+  +LQ+KF+ VA+ I+ K
Sbjct: 781 VLVTAIETLRGYVDIYEGLPSFPEIFLPISTLLLEVAKQQNLSAILQDKFKDVAQLIKKK 840

Query: 841 TEEHYMGRQPLRMRKQKTAPIKLLNPKFEENFVRGRDYDPDRERAERRKMQKLLKRETKG 887
            +EH+M R+PL+MRKQK  PIKLLNPKFEENFV+GRDYDPDRER ER+K+ KLL+RE KG
Sbjct: 841 ADEHHMLRRPLQMRKQKPVPIKLLNPKFEENFVKGRDYDPDRERVERKKLNKLLRREAKG 900

BLAST of Cucsa.327990 vs. NCBI nr
Match: gi|590698772|ref|XP_007045791.1| (Nop14, putative isoform 1 [Theobroma cacao])

HSP 1 Score: 956.8 bits (2472), Expect = 2.6e-275
Identity = 522/969 (53.87%), Postives = 650/969 (67.08%), Query Frame = 1

Query: 1   MAKLSVRSSSNNDKKGKKKKKKSSGPKALTMKVSAPKANPFESIWSHRKFDVLGKKRKGE 60
           MAK S  S +   KK KKK  K SGP A++MK+ A K+NPFE+IWS RKFD+LGKKRKGE
Sbjct: 43  MAKHS-GSDAKTKKKAKKKGSKKSGPDAISMKLKAEKSNPFETIWSRRKFDILGKKRKGE 102

Query: 61  ERRIGLARSLAIEKRKKTLLKEYERSRKSTEFSDKRIGEWDEELGEFDKAILRSQRELKR 120
           E RIGL+RSLAI+KRKKTLLKEYE+S KS+ F D RIGE ++ELGEF+K I+RSQRE + 
Sbjct: 103 ELRIGLSRSLAIQKRKKTLLKEYEQSTKSSVFVDNRIGEQNDELGEFEKGIMRSQRERQL 162

Query: 121 KLSKSSKFNLSDGEEDDYFGTQNLGALPANDDFEDEIIPDDDEDEAAAAETNKGAY---- 180
           K  K SKFNLSDGE+DD F     G+LP  DDFEDEI+ DDD D+   A   + A     
Sbjct: 163 KFGKKSKFNLSDGEDDD-FDAPGFGSLPERDDFEDEILSDDDNDDRGGATNKRSAILKQL 222

Query: 181 -----RDTQQKGRLLEGEDAVRLLAVYSFPFYFIKRKSKKEVMEEVIAKSKFFKAQKAKD 240
                +D  ++G L+EGE+               K K+KKE+MEEVI KSK+FKAQKAKD
Sbjct: 223 NSHGAQDPTERG-LVEGEEN--------------KHKTKKEIMEEVILKSKYFKAQKAKD 282

Query: 241 KEENEQLVEELDKKFETLVQSEALLSLTGSGNSNALKALVQKSVSNEHLKKDNLPAAGKT 300
           KEENEQL+EELDK F +LVQS+ LLS+T  G  NALKALV K V NEHL K+ LP + + 
Sbjct: 283 KEENEQLMEELDKNFTSLVQSQVLLSMTEPGKINALKALVNKGVLNEHLNKEELPVSQRE 342

Query: 301 ETFNQ-------------------------------------------EERQKRMLAPDN 360
           E + Q                                           EERQKRMLA D 
Sbjct: 343 EAYKQEQPDSYDKLVNELVLEMRARPSDRTKTPEEIAQEEREQLERLEEERQKRMLATDY 402

Query: 361 SSDEEDDDAETAFVGKQNYISGDDLGDSFTLDDERNHKKGWVDDILRRKDADGTESEGDD 420
           SSDE+ ++ E   + +   ISGDDLGDSF LD+E   KKGWVD+IL RKD D   SE  +
Sbjct: 403 SSDEDGENVEKDPLQRPRAISGDDLGDSFALDEEPGSKKGWVDEILERKDEDENASEDSE 462

Query: 421 SAEDSDDSQDDEDGDSDDESEEDDSNHGVKQSLKDWEQSDDDILDTESEDDDEASEGGKQ 480
           SAED+        G+ +   E+DD  H    SLK WEQSDDD L T+ ++D+E  E    
Sbjct: 463 SAEDT--------GEDEGSEEDDDDEHEKTLSLKYWEQSDDDNLGTDLDEDEEEQE---H 522

Query: 481 QDEVHPKGKVDHEGPKKAHKRSIAKS------------------------------KSFD 540
            D V  +  V+ +G  K++K  + K                               +S +
Sbjct: 523 DDTVGDEEDVEQKGCNKSNKTELKKDDGQYVDAKKIKPSIKHTSTKSDIPFIFEAPRSLE 582

Query: 541 QFLSLLANCSNSDIILIIGRIRASNAIQLTDKNLEKMQRFYGILLQYFAVSANKKPLNVE 600
           +  SLL NCSN D+I+II RIR S+AI+L  +N +KMQ FYG+LLQYFAV ANKKPLN E
Sbjct: 583 ELSSLLENCSNGDVIVIINRIRKSDAIKLAAENRKKMQVFYGVLLQYFAVLANKKPLNFE 642

Query: 601 LLNLLFKPLMEMSMEIPFYAATCARMRISHTHQHFCVQNKSPENSLWPSSKTLILLRLWT 660
           LLNLL KPLME+SMEIP+++A CAR RI  T   FC   K+ EN  WP+ KTL LLRLW+
Sbjct: 643 LLNLLVKPLMELSMEIPYFSAICARQRILRTRTQFCEALKNQENGCWPTLKTLFLLRLWS 702

Query: 661 MIFPCSDYNHVVITPTILLMCEYLMRCPILTCRDIAIGAFLCSLLLSVARQSSKFCPEAI 720
           M+FPCSD+ HVV+TP ILLMCEYLMRCPI + RD+AIG+FLCS++L V +QS KFCPEAI
Sbjct: 703 MVFPCSDFRHVVMTPAILLMCEYLMRCPITSGRDVAIGSFLCSMVLMVTKQSRKFCPEAI 762

Query: 721 NFLRTLLAAAVSSS-SSSQNPQICHLVDSQALGKLLHIQNPTNEITPLDFFFIMNLNEDS 780
            FLRTLL AA     ++ Q+ Q  +L++ +AL  LL + +  +EI PL+F  +M++ +DS
Sbjct: 763 MFLRTLLMAATDQKLAAEQDCQFYNLMELKALRPLLRVHDCVDEINPLNFLMVMDMPDDS 822

Query: 781 LVFSSDNFRAGLLSTVTETLDGFVNIYGQLKSFPEIFLPISTILHELAQQENMPDVLQNK 840
             FSSDNFRA  L TV ETL GFV IY  L SFPEIFLPI+T+L E++QQ+++P+ L++K
Sbjct: 823 SFFSSDNFRASALVTVIETLRGFVEIYDGLNSFPEIFLPIATLLLEVSQQKHIPEALKDK 882

Query: 841 FRKVAEAIEAKTEEHYMGRQPLRMRKQKTAPIKLLNPKFEENFVRGRDYDPDRERAERRK 887
           F  VA+ I+ K +E +  R+PL++RKQK  PIKLLNPKFEENFV+GRDYDPDRE+AERRK
Sbjct: 883 FNDVAQLIKQKADEAHRLRRPLQIRKQKPVPIKLLNPKFEENFVKGRDYDPDREQAERRK 942