Cucsa.321690 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.321690 vs. Swiss-Prot
Match: P2C29_ARATH (Protein phosphatase 2C 29 OS=Arabidopsis thaliana GN=PLL1 PE=1 SV=2)

HSP 1 Score: 827.8 bits (2137), Expect = 1.0e-238
Identity = 469/832 (56.37%), Postives = 566/832 (68.03%), Query Frame = 1

Query: 1   MGSGVSSLFSCLKPETR-----PAALPADNSDILFSASDPLDETLGHSFCYV-RSSNRFL 60
           MGSG SSL  C     R      +A    +SD++ S  +PLDETLGHS+CYV  SSNRF+
Sbjct: 1   MGSGFSSLLPCFNQGHRNRRRHSSAANPSHSDLIDSFREPLDETLGHSYCYVPSSSNRFI 60

Query: 61  SPSHSDRFLSPSQSLRFSPPHDPSSLPTTTTRTRTGPPETAFKAISGASVSANSSIPRSV 120
           SP  SDRF+SP+ S R SPPH+P  +  + +  +     T F+AISGASVSAN+S  ++V
Sbjct: 61  SPFPSDRFVSPTASFRLSPPHEPGRIRGSGSSEQL---HTGFRAISGASVSANTSNSKTV 120

Query: 121 LMLDAVYDDATDTALGASAGGCGVRGSILN--GFESTSSFTALPLQPVPRGGTEPLERGG 180
           L L+ +YDDAT+++ G      GVR S++N  GFE TSSF+ALPLQP P       +R G
Sbjct: 121 LQLEDIYDDATESSFGG-----GVRRSVVNANGFEGTSSFSALPLQPGP-------DRSG 180

Query: 181 FFLSGPIERGALSGPLDANVDAAAAAAAAGGGSGGGRVHFSAPLGGMYVKKKRKKGISGF 240
            F+SGPIERGA SGPLD      + + +AG       VHFSAPLGG+Y KK+RKK     
Sbjct: 181 LFMSGPIERGATSGPLDPPAGEISRSNSAG-------VHFSAPLGGVYSKKRRKKKKKSL 240

Query: 241 R-KAFTRNFSDKRPWVVPVLNFV-GRKESSTAGDEPEVR----NESDVQWALGKAGEDRV 300
                      +RPWV+PV NFV G K+ +    + E       E+D+QWALGKAGEDRV
Sbjct: 241 SWHPIFGGEKKQRPWVLPVSNFVVGAKKENIVRPDVEAMAASSGENDLQWALGKAGEDRV 300

Query: 301 HVVVSEEQGWLFVGIYDGFNGPDAPEFLMGNLYRAVFNELQGLFWEIDDNEEAAANSNPP 360
            + V E+QGWLF GIYDGFNGPDAPEFLM NLYRAV +ELQGLFWE+++ ++   + +  
Sbjct: 301 QLAVFEKQGWLFAGIYDGFNGPDAPEFLMANLYRAVHSELQGLFWELEEEDDNPTDISTR 360

Query: 361 NVAGESTVGVRVNETNADPSSRPSSQTIQTNGNISVVGNEVEVEQPAADRGSAKRVTFQS 420
            +  +      VNE  +  SS P++              E E E+        KR+T  S
Sbjct: 361 ELEQQGEFEDHVNEMAS--SSCPAT--------------EKEEEE------MGKRLT-SS 420

Query: 421 EDVPENRRRRRLWEYLAEDDTQDGLDLSGSDRFAFSVDDALSVNSAGSVAGRRWLLLSKL 480
            +V E + R+RLWE LAE   +D LDLSGSDRFAFSVDDA+   +A SV  +RWLLLSKL
Sbjct: 421 LEVVEVKERKRLWELLAEAQAEDALDLSGSDRFAFSVDDAIGAGNAVSVGSKRWLLLSKL 480

Query: 481 RQGLSKHKEGHVKTLFPWKYGLGDKEKADETENRVEETSYRSGKRRKEGLIDHELVLGAL 540
           +QGLSK      K LFPWK G+ + E  +     VEE   +  KRRK G +DHELVL A+
Sbjct: 481 KQGLSKQGISGRK-LFPWKSGVEENETEEVDNVGVEEGVDKRRKRRKAGTVDHELVLKAM 540

Query: 541 SRALEITELAYLDMTDKLLDTNPELALMGSCLLVVLMRDEDVYVMNLGDSRAIVAQYEQQ 600
           S  LE TE A+L+MTDK+L+TNPELALMGSCLLV LMRD+DVY+MN+GDSRA+VAQY+ +
Sbjct: 541 SNGLEATEQAFLEMTDKVLETNPELALMGSCLLVALMRDDDVYIMNIGDSRALVAQYQVE 600

Query: 601 EIGPSEDMKEEGHKRTGM----EGIIEESTTSEGKITPTNQPSAQTTRLTALQLSTDHST 660
           E G S +  E   +R       +G  E         T  N+     T+L ALQL+TDHST
Sbjct: 601 ETGESVETAERVEERRNDLDRDDGNKEPLVVDSSDSTVNNEAPLPQTKLVALQLTTDHST 660

Query: 661 SIEEEVRRIKNEHPDDKQCIVNDRVKGRLKVTRAFGAGFLKQPKLNDALLEMFRNEYIGT 720
           SIE+EV RIKNEHPDD  CIVNDRVKGRLKVTRAFGAGFLKQPKLNDALLEMFRNEYIGT
Sbjct: 661 SIEDEVTRIKNEHPDDNHCIVNDRVKGRLKVTRAFGAGFLKQPKLNDALLEMFRNEYIGT 720

Query: 721 APYLSCLPSLRHHRLCPTDQFIVLSSDGLYQYLTNQEVVSYVENFMEKFPDGDPAQHLIE 780
            PY+SC PSLRH+RL   DQF+VLSSDGLYQYL+N EVVS     MEKFPDGDPAQH+I+
Sbjct: 721 DPYISCTPSLRHYRLTENDQFMVLSSDGLYQYLSNVEVVSLA---MEKFPDGDPAQHVIQ 780

Query: 781 ELLCRAARKAGMDFHELLDIPQGDRRKYHDDVTVMVISLEG-RIWKSSGKYL 814
           ELL RAA+KAGMDFHELLDIPQGDRRKYHDD TV+VI+L G RIWKSSGKYL
Sbjct: 781 ELLVRAAKKAGMDFHELLDIPQGDRRKYHDDCTVLVIALGGSRIWKSSGKYL 783

BLAST of Cucsa.321690 vs. Swiss-Prot
Match: P2C32_ARATH (Protein phosphatase 2C 32 OS=Arabidopsis thaliana GN=POL PE=1 SV=2)

HSP 1 Score: 570.1 bits (1468), Expect = 3.9e-161
Identity = 368/886 (41.53%), Postives = 508/886 (57.34%), Query Frame = 1

Query: 1   MGSGVSSLFSCLKPETRPAALPADNSDILFSASDPLDETLGHSFCYVRSS---NRFLSPS 60
           MG+G S +  C  P         D + +     +PLDE LGHSFCYVR S   +  ++PS
Sbjct: 1   MGNGTSRVVGCFVPSN-------DKNGVDLEFLEPLDEGLGHSFCYVRPSIFESPDITPS 60

Query: 61  HSDRFLSPSQ---------SLRFSPPHDPSSLPTTTTRTRTGPPETAFKAISGASVSANS 120
           +S+RF   S          S R     DPS L    ++   G  ET FKAISGASVSAN 
Sbjct: 61  NSERFTIDSSTIDSETLTGSFRNDIVDDPSFLNRHNSK---GLAETTFKAISGASVSANV 120

Query: 121 SIPRSVLMLDAVYDDATDTALGASAGGCGVRGSILNGFESTSSFTALPLQPVPRGGTEPL 180
           S  R+   +     D  + A                 FESTSSF ++PLQP+PRGG+ PL
Sbjct: 121 STARTGNQMALCSSDVLEPAAS---------------FESTSSFASIPLQPLPRGGSGPL 180

Query: 181 ERGGFFLSGPIERGALSGPLDANVDAAAAAAAAGGGSG----GGRVHFSAPLGGMYVKKK 240
                F+SGP+ERG  SGPLD N    +     G  SG      R +FSAPL   + +KK
Sbjct: 181 NG---FMSGPLERGFASGPLDRNNGFMSGPIEKGVMSGPLDVSDRSNFSAPLS--FRRKK 240

Query: 241 RK-----KGISG-FRKAFTRNFSDK--------RPWVVPV--LNFVGRKESSTAGDEPEV 300
            +     + +SG  +    R FS +        R ++ P   +++   K+    G++PE 
Sbjct: 241 PRFQRFMRSVSGPMKSTLARTFSRRSGGLSWMHRFFLHPETRVSWAVGKDGKLHGEDPES 300

Query: 301 RNESD--VQWALGKAGEDRVHVVVSEEQGWLFVGIYDGFNGPDAPEFLMGNLYRAVFNEL 360
             ES+  +QWA GKAGEDRVHVV+SEEQGWLF+GIYDGF+GPDAP+F+M +LY+A+  EL
Sbjct: 301 CLESNRNLQWAHGKAGEDRVHVVLSEEQGWLFIGIYDGFSGPDAPDFVMSHLYKAIDKEL 360

Query: 361 QGLFWEID---DNEEAAANSNPP---NVAGESTVGVRVNETNADPSSRPSSQTIQTNGNI 420
           +GL W+ +   ++ +   +  PP   N+    ++  + +++    S       I + GN 
Sbjct: 361 EGLLWDYEEPSEDNQLQPDQEPPTEENMCDPESISEQHSKSVVAESEEVMIDDISSLGN- 420

Query: 421 SVVGNEVEVEQPAADRGSAKR----------VTFQSEDVPENRRRRRLWEYLAEDDTQD- 480
                ++    P    G  K+            ++ E++   R    +      +  ++ 
Sbjct: 421 --TDTQIADGPPGDSAGPGKKSMRLYELLQLEQWEGEEIGLKRYGGNVALNNMTNQVENP 480

Query: 481 -------GLDLSGSDRFAFSVDDALSVNSAGSVAGRRWLLLSKLRQGLSKHKEGHVKTLF 540
                  G D   +DR A    D +  NS      ++  + SK+R+   K K    K LF
Sbjct: 481 STSGGGAGNDPCTTDRSAL---DGIP-NSGQRHGTKKSQISSKIRRMYQKQKSLR-KKLF 540

Query: 541 PWKYGLGDKEKADETENRVEETSYRSGKRRKEGLIDHELVLGALSRALEITELAYLDMTD 600
           PW Y    +E+    E ++ E+S    +RR  G +DH+ VL A++RALE TE AY+DM +
Sbjct: 541 PWSYD-WHREEGICVEEKIVESS-GPIRRRWSGTVDHDAVLRAMARALESTEEAYMDMVE 600

Query: 601 KLLDTNPELALMGSCLLVVLMRDEDVYVMNLGDSRAIVAQYEQQEIGPSEDMKEE---GH 660
           K LD NPELALMGSC+LV+LM+D+DVYVMN+GDSRAI+AQ    +   +     +   GH
Sbjct: 601 KSLDINPELALMGSCVLVMLMKDQDVYVMNVGDSRAILAQERLHDRHSNPGFGNDEGIGH 660

Query: 661 KRTGMEGI-------IEESTTSEGKITP------TNQPSAQTTRLTALQLSTDHSTSIEE 720
           K    E +       I E +    + TP          ++   ++ A+QLS+DHSTS+EE
Sbjct: 661 KSRSRESLVRIELDRISEESPIHNQATPISVSNKNRDVTSYRLKMRAVQLSSDHSTSVEE 720

Query: 721 EVRRIKNEHPDDKQCIVNDRVKGRLKVTRAFGAGFLKQPKLNDALLEMFRNEYIGTAPYL 780
           E+ RI++EHP+D Q I+ DRVKG+LKVTRAFGAGFLK+P  N+ALLEMF+ EYIGT PY+
Sbjct: 721 EIWRIRSEHPEDDQSILKDRVKGQLKVTRAFGAGFLKKPNFNEALLEMFQVEYIGTDPYI 780

Query: 781 SCLPSLRHHRLCPTDQFIVLSSDGLYQYLTNQEVVSYVENFMEKFPDGDPAQHLIEELLC 813
           +C P   HHRL  +D+F+VLSSDGLY+Y +N+EVV++V  F+E  P+GDPAQ+LI ELL 
Sbjct: 781 TCEPCTVHHRLTSSDRFMVLSSDGLYEYFSNEEVVAHVTWFIENVPEGDPAQYLIAELLS 840

BLAST of Cucsa.321690 vs. Swiss-Prot
Match: P2C26_ORYSJ (Probable protein phosphatase 2C 26 OS=Oryza sativa subsp. japonica GN=Os02g0690500 PE=2 SV=1)

HSP 1 Score: 392.9 bits (1008), Expect = 8.6e-108
Identity = 224/404 (55.45%), Postives = 263/404 (65.10%), Query Frame = 1

Query: 416 RRLWEYLAEDDTQDG-LDLSGSDRFAFSVDDALSVNSAGSVAGRRWLLLSKLRQGLSKHK 475
           ++LW++L + D  D  LD SGS RFA                    L L +L++      
Sbjct: 248 KKLWQFLVDGDDDDSELDFSGSGRFA--------------------LSLDRLKES----- 307

Query: 476 EGHVKTLFPWKYGLGDKEKADETENRVEETSYRSGKRRKEGLIDHELVLGALSRALEITE 535
                    W +   D+   +    R+              + DH  VL AL+RAL  TE
Sbjct: 308 -----RFHMWAHAAADESGREWGSRRLAPAP---------AVRDHAAVLAALTRALASTE 367

Query: 536 LAYLDMTDKLLDTNPELALMGSCLLVVLMRDEDVYVMNLGDSRAIVAQYEQQE-----IG 595
            AYLDMTD+ + T+PELA+ G+CLLV L+RD++VYVMNLGDSRAIVAQ           G
Sbjct: 368 AAYLDMTDQSMGTHPELAVTGACLLVALVRDDNVYVMNLGDSRAIVAQRPDDGDDGCVFG 427

Query: 596 PSEDMKEEGHKRTGMEGIIEESTTSEGKITPTNQPSAQTTRLTALQLSTDHSTSIEEEVR 655
               M++ G    G+E  IE           T         L  LQLSTDHSTSIEEEV 
Sbjct: 428 TMRRMEDVG---VGLE--IE-----------TRPGGCAIIGLKPLQLSTDHSTSIEEEVH 487

Query: 656 RIKNEHPDDKQCIVNDRVKGRLKVTRAFGAGFLKQPKLNDALLEMFRNEYIGTAPYLSCL 715
           RIK EHPDD QCIVNDRVKGRLKVTRAFGAG+LKQ KLN+ LLEMFRN+YIG  PY+SC 
Sbjct: 488 RIKREHPDDDQCIVNDRVKGRLKVTRAFGAGYLKQAKLNNGLLEMFRNDYIGDTPYISCT 547

Query: 716 PSLRHHRLCPTDQFIVLSSDGLYQYLTNQEVVSYVENFMEKFPDGDPAQHLIEELLCRAA 775
           PSL HH+L   DQF+VLSSDGLYQYL+N+EVV +VENFME+FP+GDPAQ LIEELL RAA
Sbjct: 548 PSLCHHKLTARDQFLVLSSDGLYQYLSNEEVVLHVENFMERFPEGDPAQSLIEELLSRAA 596

Query: 776 RKAGMDFHELLDIPQGDRRKYHDDVTVMVISLEGRIWKSSGKYL 814
           +KAGMDF+ELLDIPQGDRRKYHDDVTVMVISLEGRIWKSSG Y+
Sbjct: 608 KKAGMDFYELLDIPQGDRRKYHDDVTVMVISLEGRIWKSSGTYV 596

HSP 2 Score: 142.1 bits (357), Expect = 2.6e-32
Identity = 100/250 (40.00%), Postives = 128/250 (51.20%), Query Frame = 1

Query: 87  TRTGPPETAFKAISGASVSANSSIPRSVLMLDAVYDDATDTALGASAGGCGVRGSILNGF 146
           T T    +    ISGA++SANSS+P  +                ASA   GV     + F
Sbjct: 43  TATAHSSSFRHGISGAALSANSSVPVPLY--------------NASAAAGGVAPGYSSAF 102

Query: 147 ESTSSFTALPLQPVPRGGTEPLERGGFFLSGPIER-GALSGPLDANVDAAAAAAAAGGGS 206
            ++SSF++ PLQ         L  G  FLSGPI+R G LSGPLD  V             
Sbjct: 103 HTSSSFSSAPLQ------LSNLSSGPLFLSGPIDRAGQLSGPLDPAVP------------ 162

Query: 207 GGGRVHFSAPLGGMYVKKKRKKGISGFRKAFTRNFSDKRPWVVPVLNFVGRKESSTAGDE 266
                 FS PL       K  K  S   + F+R F  ++P    +   V  K    A   
Sbjct: 163 ------FSGPLPA-----KPPKPASSSSRGFSRRF--RKPSFGSLRRSVSEKNRPCA--- 222

Query: 267 PEVRNESDVQWALGKAGEDRVHVVVSEEQGWLFVGIYDGFNGPDAPEFLMGNLYRAVFNE 326
             +R +  VQWA G+AGEDRVHVVVSE+Q WLFVGIYDGFNGP+AP+FL+ NLYR +  E
Sbjct: 223 VPLRRDDGVQWAHGRAGEDRVHVVVSEDQRWLFVGIYDGFNGPEAPDFLVTNLYRFLLRE 244

Query: 327 LQGLFWEIDD 336
           L+G+F++  D
Sbjct: 283 LRGIFYKEAD 244

BLAST of Cucsa.321690 vs. Swiss-Prot
Match: P2C04_ARATH (Probable protein phosphatase 2C 4 OS=Arabidopsis thaliana GN=PLL5 PE=2 SV=1)

HSP 1 Score: 359.8 bits (922), Expect = 8.1e-98
Identity = 187/319 (58.62%), Postives = 239/319 (74.92%), Query Frame = 1

Query: 490 DKEKADETENRVEETSYRSGKRRKEGLIDHELVLGALSRALEITELAYLDMTDKLLDTNP 549
           D+E  D      E+ S RSG  R   L +H  VL ALS+AL  TE AYLD  DK+LD NP
Sbjct: 353 DRESQDLDRRLKEQISRRSGSDR---LTNHSEVLEALSQALRKTEEAYLDTADKMLDENP 412

Query: 550 ELALMGSCLLVVLMRDEDVYVMNLGDSRAIVAQYEQQEIGPSEDMKEEGHKRTGMEGIIE 609
           ELALMGSC+LV+LM+ ED+YVMN+GDSRA++ Q  + +   ++        R  +E I E
Sbjct: 413 ELALMGSCVLVMLMKGEDIYVMNVGDSRAVLGQKSEPDYWLAKI-------RQDLERINE 472

Query: 610 ESTTSEGKITPTNQPSAQTTRLTALQLSTDHSTSIEEEVRRIKNEHPDDKQCIVNDRVKG 669
           E+  ++ +    +Q S+    L+A QL+ DHST+IEEEV RI+NEHPDD   + N+RVKG
Sbjct: 473 ETMMNDLEGCEGDQ-SSLVPNLSAFQLTVDHSTNIEEEVERIRNEHPDDVTAVTNERVKG 532

Query: 670 RLKVTRAFGAGFLKQPKLNDALLEMFRNEYIGTAPYLSCLPSLRHHRLCPTDQFIVLSSD 729
            LKVTRAFGAGFLKQPK N+ALLEMF+ +Y+G +PY++CLPSL HHRL   D+F++LSSD
Sbjct: 533 SLKVTRAFGAGFLKQPKWNNALLEMFQIDYVGKSPYINCLPSLYHHRLGSKDRFLILSSD 592

Query: 730 GLYQYLTNQEVVSYVENFMEKFPDGDPAQHLIEELLCRAARKAGMDFHELLDIPQGDRRK 789
           GLYQY TN+E VS VE F+   P+GDPAQHL++ELL RAA+KAGMDFHELL+IPQG+RR+
Sbjct: 593 GLYQYFTNEEAVSEVELFITLQPEGDPAQHLVQELLFRAAKKAGMDFHELLEIPQGERRR 652

Query: 790 YHDDVTVMVISLEGRIWKS 809
           YHDDV+++VISLEGR+WKS
Sbjct: 653 YHDDVSIVVISLEGRMWKS 660

HSP 2 Score: 181.8 bits (460), Expect = 3.0e-44
Identity = 138/395 (34.94%), Postives = 187/395 (47.34%), Query Frame = 1

Query: 1   MGSGVSSLFSCLKPETRPAALPADNSDILFSASDPLDETLGHSFCYVRSSNRFLSPSHSD 60
           MG+GV+ L  C          P D S +L    DPLDE LGHSFCYVR            
Sbjct: 1   MGNGVTKLSICFTGGGGERLRPKDISVLL---PDPLDEGLGHSFCYVRP----------- 60

Query: 61  RFLSPSQSLRFSPPHDPSSLPTTTTRTRTGPPETAFKAISGASVSANSSIPRSVLMLDAV 120
                          DP+ + ++   +      T F+ ISGASVSAN++ P S  + D  
Sbjct: 61  ---------------DPTLISSSKVHSEEDTTTTTFRTISGASVSANTATPLSTSLYDP- 120

Query: 121 YDDATDTALGASAGGCGVRGSILNGFESTSSFTALPLQPVPRG------GTEPLERGGFF 180
           Y      A                 FEST+SF+++PLQP+P+       G+ P+ERG  F
Sbjct: 121 YGHIDRAA----------------AFESTTSFSSIPLQPIPKSSGPIVLGSGPIERG--F 180

Query: 181 LSGPIERGALSGPLDANVDAAAAAAAAGGGSGGGRVHFSAPLG-GMYVKKKRKKGISGFR 240
           LSGPIERG +SGPLD  V   +            +   S   G  + V  +++  +   R
Sbjct: 181 LSGPIERGFMSGPLD-RVGLFSGPLDKPNSDHHHQFQRSFSHGLALRVGSRKRSLVRILR 240

Query: 241 KAFTRNFSDKRPWVVPVLNFVGRKESSTAGDEPE----VRNES----------------- 300
           +A ++  S  +  +V  +  V  K+S   G   E    + NE+                 
Sbjct: 241 RAISKTMSRGQNSIVAPIKSV--KDSDNWGIRSEKSRNLHNENLTVNSLNFSSEVSLDDD 300

Query: 301 ------DVQWALGKAGEDRVHVVVSEEQGWLFVGIYDGFNGPDAPEFLMGNLYRAVFNEL 360
                 ++QWA GKAGEDRVHVVVSEE GWLFVGIYDGFNGPDAP++L+ +LY  V  EL
Sbjct: 301 VSLENQNLQWAQGKAGEDRVHVVVSEEHGWLFVGIYDGFNGPDAPDYLLSHLYPVVHREL 343

Query: 361 QGLFWEIDDNEEAAANSNPPNVAGESTVGVRVNET 362
           +GL W+ D N E+ +     +   ES      +ET
Sbjct: 361 KGLLWD-DSNVESKSQDLERSNGDESCSNQEKDET 343

BLAST of Cucsa.321690 vs. Swiss-Prot
Match: P2C66_ARATH (Probable protein phosphatase 2C 66 OS=Arabidopsis thaliana GN=PLL2 PE=2 SV=1)

HSP 1 Score: 345.5 bits (885), Expect = 1.6e-93
Identity = 182/326 (55.83%), Postives = 233/326 (71.47%), Query Frame = 1

Query: 484 WKYGLGDKEKADETENRVEETSYRSGKRRKEGLIDHELVLGALSRALEITELAYLDMTDK 543
           W+    +K K+D   NR ++      K       +H+ VL AL +AL  TE AYL++ D+
Sbjct: 368 WEKKSNNKTKSD---NRCDQ------KGSNSTTTNHKDVLKALLQALRKTEDAYLELADQ 427

Query: 544 LLDTNPELALMGSCLLVVLMRDEDVYVMNLGDSRAIVAQYEQQEIGPSEDMKEEGHKRTG 603
           ++  NPELALMGSC+LV LM+ EDVYVMN+GDSRA+        +G   ++     ++  
Sbjct: 428 MVKENPELALMGSCVLVTLMKGEDVYVMNVGDSRAV--------LGRKPNLATGRKRQKE 487

Query: 604 MEGIIEESTTSEGKITPTNQPSAQTTRLTALQLSTDHSTSIEEEVRRIKNEHPDDKQCIV 663
           +E I E+S+  + +I       A    L  LQL+ +HST IEEEVRRIK EHPDD   + 
Sbjct: 488 LERIREDSSLEDKEILMNG---AMRNTLVPLQLNMEHSTRIEEEVRRIKKEHPDDDCAVE 547

Query: 664 NDRVKGRLKVTRAFGAGFLKQPKLNDALLEMFRNEYIGTAPYLSCLPSLRHHRLCPTDQF 723
           NDRVKG LKVTRAFGAGFLKQPK NDALLEMFR +YIGT+PY++C PSL HH+L   D+F
Sbjct: 548 NDRVKGYLKVTRAFGAGFLKQPKWNDALLEMFRIDYIGTSPYITCSPSLCHHKLTSRDKF 607

Query: 724 IVLSSDGLYQYLTNQEVVSYVENFMEKFPDGDPAQHLIEELLCRAARKAGMDFHELLDIP 783
           ++LSSDGLY+Y +NQE +  VE+F+  FP+GDPAQHLI+E+L RAA K GMDFHELL+IP
Sbjct: 608 LILSSDGLYEYFSNQEAIFEVESFISAFPEGDPAQHLIQEVLLRAANKFGMDFHELLEIP 667

Query: 784 QGDRRKYHDDVTVMVISLEGRIWKSS 810
           QGDRR+YHDDV+V+VISLEGRIW+SS
Sbjct: 668 QGDRRRYHDDVSVIVISLEGRIWRSS 673

HSP 2 Score: 151.8 bits (382), Expect = 3.3e-35
Identity = 127/373 (34.05%), Postives = 165/373 (44.24%), Query Frame = 1

Query: 1   MGSGVSSLFSCLKPETRPAALPADNSDILFSASDPLDETLGHSFCYVRSSNRFLSPSHSD 60
           MG+GV++L  C       A   +   D+       + + LGHSFCY+R            
Sbjct: 1   MGNGVTTLTGCCTGTL--AGEISRRYDVSL-----VHDGLGHSFCYIR------------ 60

Query: 61  RFLSPSQSLRFSPPHDPSSLPTTTTRTRTGP-PETAFKAISGASVSANSSIPRSVLMLDA 120
                       P      LP+  +  R+    ET F++ISGASVSAN S   S   L +
Sbjct: 61  ------------PDLPGVVLPSPESPLRSDHIQETTFRSISGASVSANPSTALSGA-LSS 120

Query: 121 VYDDATDTALGASAGGCGVRGSILNGFESTSSFTALPLQPVPRG--------------GT 180
             D    +A+ ASA            FES+ +F +LPLQPVPRG              G+
Sbjct: 121 DSDCPYSSAVSASA------------FESSGNFASLPLQPVPRGSTWQSGPIVNESGLGS 180

Query: 181 EPLERGGFFLSGPIERGALSGPLDANVDAAAAAAAAGGGSGGGRVHFSAPLGGMYVKKKR 240
            P ER   FLSGPIE G  SGP+++                            +  K K 
Sbjct: 181 APFERR--FLSGPIESGLYSGPIESTKKTEKEKPKK-----------------IRKKPKS 240

Query: 241 KKGISGFRKAFTRNFSDK-----RPWVVPVLNFVGRKESSTAGDEPEVR----NES---- 300
           KK    F+  F    S+      +  V+  +N     +S     EP +     NE+    
Sbjct: 241 KKNFLTFKTLFANLISNNNKPRLKKSVIEPINGSDSSDSGRLHHEPVITSSRSNENPKSD 300

Query: 301 --------------DVQWALGKAGEDRVHVVVSEEQGWLFVGIYDGFNGPDAPEFLMGNL 332
                         DVQWA GKAGEDRVHVVVSE+ GW+FVGIYDGF+GPDAP++L+ NL
Sbjct: 301 LEEEDEKQSMNSVLDVQWAQGKAGEDRVHVVVSEDNGWVFVGIYDGFSGPDAPDYLLNNL 310

BLAST of Cucsa.321690 vs. TrEMBL
Match: A0A0A0L597_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G011830 PE=4 SV=1)

HSP 1 Score: 1626.7 bits (4211), Expect = 0.0e+00
Identity = 813/813 (100.00%), Postives = 813/813 (100.00%), Query Frame = 1

Query: 1   MGSGVSSLFSCLKPETRPAALPADNSDILFSASDPLDETLGHSFCYVRSSNRFLSPSHSD 60
           MGSGVSSLFSCLKPETRPAALPADNSDILFSASDPLDETLGHSFCYVRSSNRFLSPSHSD
Sbjct: 1   MGSGVSSLFSCLKPETRPAALPADNSDILFSASDPLDETLGHSFCYVRSSNRFLSPSHSD 60

Query: 61  RFLSPSQSLRFSPPHDPSSLPTTTTRTRTGPPETAFKAISGASVSANSSIPRSVLMLDAV 120
           RFLSPSQSLRFSPPHDPSSLPTTTTRTRTGPPETAFKAISGASVSANSSIPRSVLMLDAV
Sbjct: 61  RFLSPSQSLRFSPPHDPSSLPTTTTRTRTGPPETAFKAISGASVSANSSIPRSVLMLDAV 120

Query: 121 YDDATDTALGASAGGCGVRGSILNGFESTSSFTALPLQPVPRGGTEPLERGGFFLSGPIE 180
           YDDATDTALGASAGGCGVRGSILNGFESTSSFTALPLQPVPRGGTEPLERGGFFLSGPIE
Sbjct: 121 YDDATDTALGASAGGCGVRGSILNGFESTSSFTALPLQPVPRGGTEPLERGGFFLSGPIE 180

Query: 181 RGALSGPLDANVDAAAAAAAAGGGSGGGRVHFSAPLGGMYVKKKRKKGISGFRKAFTRNF 240
           RGALSGPLDANVDAAAAAAAAGGGSGGGRVHFSAPLGGMYVKKKRKKGISGFRKAFTRNF
Sbjct: 181 RGALSGPLDANVDAAAAAAAAGGGSGGGRVHFSAPLGGMYVKKKRKKGISGFRKAFTRNF 240

Query: 241 SDKRPWVVPVLNFVGRKESSTAGDEPEVRNESDVQWALGKAGEDRVHVVVSEEQGWLFVG 300
           SDKRPWVVPVLNFVGRKESSTAGDEPEVRNESDVQWALGKAGEDRVHVVVSEEQGWLFVG
Sbjct: 241 SDKRPWVVPVLNFVGRKESSTAGDEPEVRNESDVQWALGKAGEDRVHVVVSEEQGWLFVG 300

Query: 301 IYDGFNGPDAPEFLMGNLYRAVFNELQGLFWEIDDNEEAAANSNPPNVAGESTVGVRVNE 360
           IYDGFNGPDAPEFLMGNLYRAVFNELQGLFWEIDDNEEAAANSNPPNVAGESTVGVRVNE
Sbjct: 301 IYDGFNGPDAPEFLMGNLYRAVFNELQGLFWEIDDNEEAAANSNPPNVAGESTVGVRVNE 360

Query: 361 TNADPSSRPSSQTIQTNGNISVVGNEVEVEQPAADRGSAKRVTFQSEDVPENRRRRRLWE 420
           TNADPSSRPSSQTIQTNGNISVVGNEVEVEQPAADRGSAKRVTFQSEDVPENRRRRRLWE
Sbjct: 361 TNADPSSRPSSQTIQTNGNISVVGNEVEVEQPAADRGSAKRVTFQSEDVPENRRRRRLWE 420

Query: 421 YLAEDDTQDGLDLSGSDRFAFSVDDALSVNSAGSVAGRRWLLLSKLRQGLSKHKEGHVKT 480
           YLAEDDTQDGLDLSGSDRFAFSVDDALSVNSAGSVAGRRWLLLSKLRQGLSKHKEGHVKT
Sbjct: 421 YLAEDDTQDGLDLSGSDRFAFSVDDALSVNSAGSVAGRRWLLLSKLRQGLSKHKEGHVKT 480

Query: 481 LFPWKYGLGDKEKADETENRVEETSYRSGKRRKEGLIDHELVLGALSRALEITELAYLDM 540
           LFPWKYGLGDKEKADETENRVEETSYRSGKRRKEGLIDHELVLGALSRALEITELAYLDM
Sbjct: 481 LFPWKYGLGDKEKADETENRVEETSYRSGKRRKEGLIDHELVLGALSRALEITELAYLDM 540

Query: 541 TDKLLDTNPELALMGSCLLVVLMRDEDVYVMNLGDSRAIVAQYEQQEIGPSEDMKEEGHK 600
           TDKLLDTNPELALMGSCLLVVLMRDEDVYVMNLGDSRAIVAQYEQQEIGPSEDMKEEGHK
Sbjct: 541 TDKLLDTNPELALMGSCLLVVLMRDEDVYVMNLGDSRAIVAQYEQQEIGPSEDMKEEGHK 600

Query: 601 RTGMEGIIEESTTSEGKITPTNQPSAQTTRLTALQLSTDHSTSIEEEVRRIKNEHPDDKQ 660
           RTGMEGIIEESTTSEGKITPTNQPSAQTTRLTALQLSTDHSTSIEEEVRRIKNEHPDDKQ
Sbjct: 601 RTGMEGIIEESTTSEGKITPTNQPSAQTTRLTALQLSTDHSTSIEEEVRRIKNEHPDDKQ 660

Query: 661 CIVNDRVKGRLKVTRAFGAGFLKQPKLNDALLEMFRNEYIGTAPYLSCLPSLRHHRLCPT 720
           CIVNDRVKGRLKVTRAFGAGFLKQPKLNDALLEMFRNEYIGTAPYLSCLPSLRHHRLCPT
Sbjct: 661 CIVNDRVKGRLKVTRAFGAGFLKQPKLNDALLEMFRNEYIGTAPYLSCLPSLRHHRLCPT 720

Query: 721 DQFIVLSSDGLYQYLTNQEVVSYVENFMEKFPDGDPAQHLIEELLCRAARKAGMDFHELL 780
           DQFIVLSSDGLYQYLTNQEVVSYVENFMEKFPDGDPAQHLIEELLCRAARKAGMDFHELL
Sbjct: 721 DQFIVLSSDGLYQYLTNQEVVSYVENFMEKFPDGDPAQHLIEELLCRAARKAGMDFHELL 780

Query: 781 DIPQGDRRKYHDDVTVMVISLEGRIWKSSGKYL 814
           DIPQGDRRKYHDDVTVMVISLEGRIWKSSGKYL
Sbjct: 781 DIPQGDRRKYHDDVTVMVISLEGRIWKSSGKYL 813

BLAST of Cucsa.321690 vs. TrEMBL
Match: W9RNL1_9ROSA (Protein phosphatase 2C 29 OS=Morus notabilis GN=L484_023301 PE=4 SV=1)

HSP 1 Score: 1116.3 bits (2886), Expect = 0.0e+00
Identity = 593/837 (70.85%), Postives = 661/837 (78.97%), Query Frame = 1

Query: 1   MGSGVSSLFSCLKPETRPAALPA--------DNSDILFSASDPLDETLGHSFCYVRSSNR 60
           MG G+S++F C  P +R               N D++F++S+PLDETLGHSF YVRSS R
Sbjct: 1   MGGGLSTVFPCFTPASRSNHPDPHHDLHSHHQNQDLIFTSSEPLDETLGHSFVYVRSSAR 60

Query: 61  FLSPSHSDRFLSPSQSLRFSPPHDPSSLPTTTTRTRTGPPETAFKAISGASVSANSSIPR 120
           FLSP+ SDRF+SPSQSLRFSP H+P       +RTR GPPET F+ ISGASVSAN+S PR
Sbjct: 61  FLSPTQSDRFVSPSQSLRFSPSHEPG-----VSRTRAGPPETGFRTISGASVSANTSTPR 120

Query: 121 SVLMLDAVYDDATDTALGASA---GGCGVRGSILNGFESTSSFTALPLQPVPRGG----- 180
           +VL LD +YDDAT+T LG      GG GVRGSI+NGFESTSSF+ALPLQPVPRGG     
Sbjct: 121 TVLQLDNIYDDATETTLGCGGVGVGGGGVRGSIVNGFESTSSFSALPLQPVPRGGGGGGG 180

Query: 181 --TEPLERGGFFLSGPIERGALSGPLDANVDAAAAAAAAGGGSGGGRVHFSAPLGGMYVK 240
             + P+ERGGFFLSGPIERGALSGPLDAN       AA  G  G GRVHFSAPLGG YVK
Sbjct: 181 EPSGPMERGGFFLSGPIERGALSGPLDAN-------AAGAGCDGSGRVHFSAPLGGPYVK 240

Query: 241 KKRKKGISGFRKAFTRNFSDK-RPWVVPVLNFVGRKESSTAGDEPEVRNE-SDVQWALGK 300
           +KRKKGISG RKAF RNFS+K RPWVVPVLNFVGRKESS  GDE E R+E S+VQWALGK
Sbjct: 241 RKRKKGISGIRKAFYRNFSEKKRPWVVPVLNFVGRKESSGLGDESEARSETSNVQWALGK 300

Query: 301 AGEDRVHVVVSEEQGWLFVGIYDGFNGPDAPEFLMGNLYRAVFNELQGLFWEIDDNEEAA 360
           AGEDRVHVVVSEEQGWLFVGIYDGFNGPDAPEFLMGNLYRAV NELQGLFW +D+ EEA 
Sbjct: 301 AGEDRVHVVVSEEQGWLFVGIYDGFNGPDAPEFLMGNLYRAVHNELQGLFWVVDEAEEAT 360

Query: 361 ANSNPPNV-AGESTVGVRVNETNADPSSRPSSQTIQTNGNISVVGNEVEVEQPAADRGSA 420
             S+P NV A E+   V V ETN                      +EV  E         
Sbjct: 361 --SDPQNVVAEENENAVAVEETNQ---------------------SEVRTE--------- 420

Query: 421 KRVTFQSEDV--PENRRRRRLWEYLAEDDTQDGLDLSGSDRFAFSVDDALSVNSAGSVAG 480
           KRVTFQSE+   P   RRRRL ++LAEDD +DGLDLSGS RFAFSVDDAL V++ GS A 
Sbjct: 421 KRVTFQSEETERPAETRRRRLRDFLAEDDAEDGLDLSGSQRFAFSVDDALIVSNGGSAAS 480

Query: 481 RRWLLLSKLRQGLSKHKEGHVKTLFPWKYGLGDKEKAD-ETENRVEET-SYRSGKRRKEG 540
           RRWLLLSKL+QGLSKH+EGH +  FPW++GL DKEK + E ENRVEE  S R  +RRKEG
Sbjct: 481 RRWLLLSKLKQGLSKHREGHGRKFFPWRFGLEDKEKVEVEVENRVEEERSNRGSRRRKEG 540

Query: 541 LIDHELVLGALSRALEITELAYLDMTDKLLDTNPELALMGSCLLVVLMRDEDVYVMNLGD 600
            +DHELVLGALSRALE+TELAYLDMTDK+LDTNPELALMGSCLLVVLMRDEDVYVMN+GD
Sbjct: 541 PVDHELVLGALSRALEVTELAYLDMTDKVLDTNPELALMGSCLLVVLMRDEDVYVMNVGD 600

Query: 601 SRAIVAQYEQQEIGPSEDMKEEGHKRTGMEGIIEESTTS-EGKITPTNQPSAQTTRLTAL 660
           SRAIVAQYE +E+G S   + +G   +G EGI+EES  + E  +   N+  AQ  +LTAL
Sbjct: 601 SRAIVAQYEPEEVGSSAHSRGQGENGSGTEGIVEESLAAGERAMKLENETPAQAMKLTAL 660

Query: 661 QLSTDHSTSIEEEVRRIKNEHPDDKQCIVNDRVKGRLKVTRAFGAGFLKQPKLNDALLEM 720
           QLSTDHST+IEEE+ RIKNEHPDDK CIVNDRVKGRLKVTRAFGAGFLKQPK NDALLEM
Sbjct: 661 QLSTDHSTNIEEEIIRIKNEHPDDKNCIVNDRVKGRLKVTRAFGAGFLKQPKWNDALLEM 720

Query: 721 FRNEYIGTAPYLSCLPSLRHHRLCPTDQFIVLSSDGLYQYLTNQEVVSYVENFMEKFPDG 780
           FRNEYIGTAPY+SC PSLRHHRLCP DQF+VLSSDGLYQYLTN+EVVS+VENFMEKFP+G
Sbjct: 721 FRNEYIGTAPYISCSPSLRHHRLCPRDQFLVLSSDGLYQYLTNEEVVSHVENFMEKFPEG 780

Query: 781 DPAQHLIEELLCRAARKAGMDFHELLDIPQGDRRKYHDDVTVMVISLEGRIWKSSGK 812
           DPAQHLIEELL RAA+KAGMDFH+LLDIPQGDRRKYHDDVTVM+ISLEGRIWKSSGK
Sbjct: 781 DPAQHLIEELLFRAAKKAGMDFHDLLDIPQGDRRKYHDDVTVMIISLEGRIWKSSGK 793

BLAST of Cucsa.321690 vs. TrEMBL
Match: F6HTA4_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_02s0012g01100 PE=4 SV=1)

HSP 1 Score: 1094.7 bits (2830), Expect = 0.0e+00
Identity = 576/819 (70.33%), Postives = 651/819 (79.49%), Query Frame = 1

Query: 1   MGSGVSSLFSCLKPETRPAALPADNSDILFSASDPLDETLGHSFCYVRSSNRFLSPSHSD 60
           MGSG+S L  C  P +R A    +  +++F+AS+PLDETLGHSFCYVRSS RFLSP+ SD
Sbjct: 1   MGSGLSQLCPCFVPASRTAV---EEPEVVFTASEPLDETLGHSFCYVRSSARFLSPTQSD 60

Query: 61  RFLSPSQSLRFSPPHDPSSLPTTTTRTRTGPPETAFKAISGASVSANSSIPRSVLMLDAV 120
           RF+SPS SLRFSP H+P        R R GPPET FKAISGASVSAN+S PR+VL L+ +
Sbjct: 61  RFVSPSHSLRFSPSHEPVG------RARAGPPETGFKAISGASVSANTSTPRTVLQLENI 120

Query: 121 YDDATDTALGASAGGCGVRGSILNGFESTSSFTALPLQPVPRGG--TEPLERGGFFLSGP 180
           YDDATD        G GV+GSI+NGFEST+SF+ALPLQPVPRGG  + P++RG FF+SGP
Sbjct: 121 YDDATDCV------GGGVKGSIVNGFESTASFSALPLQPVPRGGEASGPIDRG-FFMSGP 180

Query: 181 IERGALSGPLDANVDAAAAAAAAGGGSGGGRVHFSAPLGGMYVKKKRKKGISGFRKAFTR 240
           IERGALSGPLDAN            GS GGRVHFSAPL G+YVKKKRKKGIS  RKAF R
Sbjct: 181 IERGALSGPLDANA-----------GSDGGRVHFSAPLNGLYVKKKRKKGISAIRKAFYR 240

Query: 241 NFSDK-RPWVVPVLNFVGRKESSTAGD--EPEVRNESDVQWALGKAGEDRVHVVVSEEQG 300
           NFS+K RPWVVPVLNFVGRKE   AGD  + EV++ES+VQWALGKAGEDRVHVVVSEE G
Sbjct: 241 NFSEKKRPWVVPVLNFVGRKEVPAAGDGGDSEVKSESNVQWALGKAGEDRVHVVVSEEHG 300

Query: 301 WLFVGIYDGFNGPDAPEFLMGNLYRAVFNELQGLFWEIDDNEEAAANSNPPNVAGESTVG 360
           WLFVGIYDGFNGPDAPEFLMGNLYRAVFNEL+GLFW++++ +   +     NV  E+ V 
Sbjct: 301 WLFVGIYDGFNGPDAPEFLMGNLYRAVFNELEGLFWDVEEADPIVSGQECSNVTSENVVI 360

Query: 361 VRVNETNADPSSRPSSQTIQTNGNISVVGNEVEVEQPAADRGSAKRVTFQSEDVPENRRR 420
           V    TN    S       ++N     VG+E +   P  DRGSAKRVTFQ E  P   RR
Sbjct: 361 VE-EATNQSMESCVGEVGNESNNKEIGVGHE-DSNPPPTDRGSAKRVTFQPE--PPEIRR 420

Query: 421 RRLWEYLAEDDTQDGLDLSGSDRFAFSVDDALSVNSAGSVAGRRWLLLSKLRQGLSKHKE 480
           RRLWE+LAEDD +DGLDLSGSDRFAFSVDDALSVN+AGS   RRWLLLSKL+ GLSKHKE
Sbjct: 421 RRLWEFLAEDDQEDGLDLSGSDRFAFSVDDALSVNNAGSAVSRRWLLLSKLKHGLSKHKE 480

Query: 481 GHVKTLFPWKYGLGDKEKADETENRVEETSYRSGKRRKEGLIDHELVLGALSRALEITEL 540
           GH + LFPW++GL DKEK  E +NRVEETS   G++RK G +DHELVL A+SRALE+TEL
Sbjct: 481 GHGRKLFPWRFGLEDKEKV-EVDNRVEETSGSRGRKRKVGPVDHELVLKAMSRALELTEL 540

Query: 541 AYLDMTDKLLDTNPELALMGSCLLVVLMRDEDVYVMNLGDSRAIVAQYEQQEIGPSEDMK 600
           AYLDMTDK+LD NPELALMGSCLLVVLMRDEDVYVMN+GDSRA+VAQYE QE   S + K
Sbjct: 541 AYLDMTDKVLDQNPELALMGSCLLVVLMRDEDVYVMNVGDSRALVAQYETQEGSSSVESK 600

Query: 601 EEGHKRTGMEGIIEESTTS-EGKITPTNQPSAQTTRLTALQLSTDHSTSIEEEVRRIKNE 660
            EG  R+ ++ I+E ST   E +    N+   +  RLTALQLSTDHSTSIEEEV RIKNE
Sbjct: 601 GEGKNRSNIDDIVEVSTDGCEKENKVVNEDFVKGMRLTALQLSTDHSTSIEEEVIRIKNE 660

Query: 661 HPDDKQCIVNDRVKGRLKVTRAFGAGFLKQPKLNDALLEMFRNEYIGTAPYLSCLPSLRH 720
           HPDD QCIVNDRVKGRLKVTRAFGAGFLKQPK ND LLEMFRNEYIGTAPY+SC PSL +
Sbjct: 661 HPDDSQCIVNDRVKGRLKVTRAFGAGFLKQPKWNDVLLEMFRNEYIGTAPYISCSPSLCY 720

Query: 721 HRLCPTDQFIVLSSDGLYQYLTNQEVVSYVENFMEKFPDGDPAQHLIEELLCRAARKAGM 780
           H+LCP DQF++LSSDGLYQYL+NQEVV  VE FMEKFP+GDPAQHLIEELL RAA+KAG+
Sbjct: 721 HQLCPRDQFLILSSDGLYQYLSNQEVVCEVEKFMEKFPEGDPAQHLIEELLSRAAKKAGL 780

Query: 781 DFHELLDIPQGDRRKYHDDVTVMVISLEGRIWKSSGKYL 814
           +FHELLDIPQGDRRKYHDDVTVMVISLEGRIWKSSGKYL
Sbjct: 781 NFHELLDIPQGDRRKYHDDVTVMVISLEGRIWKSSGKYL 787

BLAST of Cucsa.321690 vs. TrEMBL
Match: A0A061G3Q6_THECC (Poltergeist like 1 isoform 1 OS=Theobroma cacao GN=TCM_015752 PE=4 SV=1)

HSP 1 Score: 1064.3 bits (2751), Expect = 7.4e-308
Identity = 564/826 (68.28%), Postives = 648/826 (78.45%), Query Frame = 1

Query: 1   MGSGVSSLFSCLKPETRPAALPADNS-DILFSASDPLDETLGHSFCYVRSSNRFLSPSHS 60
           MGSGVS+L  C  P T  +    DN  D++F++S+PLDETLGHSFCYVRSSNRFLSP+ S
Sbjct: 1   MGSGVSTLCPCFNPVTSRSNRQPDNQPDLIFASSEPLDETLGHSFCYVRSSNRFLSPTPS 60

Query: 61  DRFLSPSQSLRFSPPHDPSSLPTTTTRTRTGPPETAFKAISGASVSANSSIPRSVLMLDA 120
           DRF+SPS SLRFSPPHDP        +TRTGP ET FKAISGASVSAN+S PR+VL LD 
Sbjct: 61  DRFVSPSHSLRFSPPHDP--------KTRTGP-ETLFKAISGASVSANTSTPRTVLQLDN 120

Query: 121 VYDDATDTALGASAGGCGVRGSIL--NGFESTSSFTALPLQPVPRGGTE-PLERGGFFLS 180
           +YDDAT+  LG      GV+ SI+  NGFESTSSF+ALPLQPVPRG    P+ERGGFF+S
Sbjct: 121 IYDDATENTLGY-----GVKSSIVSGNGFESTSSFSALPLQPVPRGVAHVPMERGGFFMS 180

Query: 181 GPIERGALSGPLDANVDAAAAAAAAGGGSGGGRVHFSAPLGGMYVKKKRKKGISGFRKAF 240
           GP+ERGA+SGPL+AN                GRVHFSAPLGG+YVKKKRKKGI+G     
Sbjct: 181 GPLERGAVSGPLEANPGPE------------GRVHFSAPLGGIYVKKKRKKGITGI---- 240

Query: 241 TRNFSDK-RPWVVPVLNFVGRKESSTAGDEPEV---RNESDVQWALGKAGEDRVHVVVSE 300
            RNFS+K RPWVVPVLNFVGR+E+S   +   V   + E DVQWALGKAGEDRVHVVVSE
Sbjct: 241 LRNFSEKKRPWVVPVLNFVGRRENSEEAEAAAVVEGKTEGDVQWALGKAGEDRVHVVVSE 300

Query: 301 EQGWLFVGIYDGFNGPDAPEFLMGNLYRAVFNELQGLFWEIDDN--EEAAANSNPPN--V 360
           EQGWLFVGIYDGFNGPDAPEFLMGNLYRAVF+EL GLFWE+D+    EA   +N P+  +
Sbjct: 301 EQGWLFVGIYDGFNGPDAPEFLMGNLYRAVFHELHGLFWELDEETENEAENKTNAPDLII 360

Query: 361 AGESTVGVRVNETNADPSSRPSSQTIQTNGNISVVGNEVEVEQPAADRGSAKRVTFQSED 420
           A +S+     N+T+ +  ++  S     +    ++G   E E     +   +RVTF+SE 
Sbjct: 361 ATDSS-----NKTDTETENQQKSVEEVKSAGEGLIG---ENESSGVVQERTRRVTFESEG 420

Query: 421 VPENRRRRRLWEYLAEDDTQDGLDLSGSDRFAFSVDDALSVNSAGSVAGRRWLLLSKLRQ 480
                RRRRLWE+LAEDD +DGLDLSGSDRFAFSVDDA+SV+  G+   RRWLLLSKL+Q
Sbjct: 421 T--EIRRRRLWEFLAEDDPEDGLDLSGSDRFAFSVDDAISVSKEGTAVSRRWLLLSKLKQ 480

Query: 481 GLSKHKEGHVKTLFPWKYGLGDKEKADETENRVEETSYRSGKRRKEGLIDHELVLGALSR 540
           GLSKH++G    LFPW++GL +KEK +E  NRVEE    +G+RRKEG +DHELVL ALSR
Sbjct: 481 GLSKHRDGSGSLLFPWRFGLEEKEKVEEVGNRVEERVQETGRRRKEGPVDHELVLRALSR 540

Query: 541 ALEITELAYLDMTDKLLDTNPELALMGSCLLVVLMRDEDVYVMNLGDSRAIVAQYEQQEI 600
           ALE+TELAYLDMTDK+L TNPELALMGSCLLVVLMRDEDVYVMN+GDSRAIVAQYE +E+
Sbjct: 541 ALEVTELAYLDMTDKVLGTNPELALMGSCLLVVLMRDEDVYVMNVGDSRAIVAQYELEEV 600

Query: 601 GPSEDMKEEGHKRTGMEGIIEESTT-SEGKITPTNQPSAQTTRLTALQLSTDHSTSIEEE 660
           G S + ++     + MEGI+EE+    E  I P ++   Q  RLTALQLSTDHSTSIEEE
Sbjct: 601 GSSMEGRKREENGSSMEGIVEETLAIGEKAIKPVSEAPPQPMRLTALQLSTDHSTSIEEE 660

Query: 661 VRRIKNEHPDDKQCIVNDRVKGRLKVTRAFGAGFLKQPKLNDALLEMFRNEYIGTAPYLS 720
           V RIKNEHPDD  CIVNDRVKGRLKVTRAFGAGFLK+PKLNDALLEMFRNEYIGTAPY+S
Sbjct: 661 VIRIKNEHPDDSHCIVNDRVKGRLKVTRAFGAGFLKKPKLNDALLEMFRNEYIGTAPYIS 720

Query: 721 CLPSLRHHRLCPTDQFIVLSSDGLYQYLTNQEVVSYVENFMEKFPDGDPAQHLIEELLCR 780
           C PS+ HHRLCP DQF++LSSDGLYQYL+NQEVVS VE+FMEKFPDGDPAQHLIEELL R
Sbjct: 721 CSPSICHHRLCPRDQFLILSSDGLYQYLSNQEVVSLVESFMEKFPDGDPAQHLIEELLFR 780

Query: 781 AARKAGMDFHELLDIPQGDRRKYHDDVTVMVISLEGRIWKSSGKYL 814
           AA+KAGMDFHELLDIPQGDRRKYHDDVTVMVISLEGRIWKSSGKYL
Sbjct: 781 AAKKAGMDFHELLDIPQGDRRKYHDDVTVMVISLEGRIWKSSGKYL 786

BLAST of Cucsa.321690 vs. TrEMBL
Match: M5WFE0_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa001785mg PE=4 SV=1)

HSP 1 Score: 1063.5 bits (2749), Expect = 1.3e-307
Identity = 573/829 (69.12%), Postives = 643/829 (77.56%), Query Frame = 1

Query: 1   MGSGVSSLFSCLKPETRPAALPADNSDILFSASDPLDETLGHSFCYVRSSNRFLSPSHSD 60
           MGSGVSSL SC +P  R      D++D++F+AS+PLDETLGHSFCYVRSS RFLSP+ SD
Sbjct: 1   MGSGVSSLSSCFRPVHRTNHHHPDHNDVVFAASEPLDETLGHSFCYVRSSARFLSPTQSD 60

Query: 61  RFLSPSQSLRFSPPHDPSSLPTTTTRTRTGPPETAFKAISGASVSANSSIPRSVLMLDAV 120
           RF+SPS SLRFSP H+P +      RTR G  ET FKAISGASVSANSS PR+VL LD +
Sbjct: 61  RFISPSNSLRFSPSHEPGA------RTRPGLHETGFKAISGASVSANSSTPRTVLQLDNI 120

Query: 121 YDDATDTALGASAGGCGVRGSILNGFESTSSFTALPLQPVPRGGTE----PLERGGFFLS 180
           YDDATD+ LG   GG  VRGSI+NGFESTSSF+ALPLQPVPRGG      P+ER GFFLS
Sbjct: 121 YDDATDSVLGGFGGG--VRGSIVNGFESTSSFSALPLQPVPRGGERDPSGPMERAGFFLS 180

Query: 181 GPIERGALSGPLDANVDAAAAAAAAGGGSGGGRVHFSAPLGGMYVKKKRKKGISGFRKAF 240
           GPIERGALSGPLD N +A             G+VHFSAPLGG+YVK++RKKGISG RKA 
Sbjct: 181 GPIERGALSGPLDPNPNAHGP---------DGQVHFSAPLGGLYVKRRRKKGISGIRKAL 240

Query: 241 TRNFSDK--RPWVVPVLNFVGRKESSTA-GDEPEVRNESDVQWALGKAGEDRVHVVVSEE 300
            RNFS+K  RPWVVPVLNFVGRKE     GDE E ++E++VQWALGKAGEDRVHVVVSEE
Sbjct: 241 YRNFSEKKQRPWVVPVLNFVGRKEGPPPPGDEAEPKSETNVQWALGKAGEDRVHVVVSEE 300

Query: 301 QGWLFVGIYDGFNGPDAPEFLMGNLYRAVFNELQGLFWEIDDNEEA-----AANSNPPNV 360
           QGWLFVGIYDGFNGPDAPEFLMGNLYRA +NELQGLFWE+D++++A     A N+NPPNV
Sbjct: 301 QGWLFVGIYDGFNGPDAPEFLMGNLYRAFYNELQGLFWEVDEDDQAPGTEEANNTNPPNV 360

Query: 361 AGESTVGVRVNETNADPSSRPSSQTIQTNGNISVVGNEVEVEQPAADRGSAKRVTFQSED 420
           A ES V V V     D +S P ++                     ADR   KRVTFQSE 
Sbjct: 361 ATESEVVVDVKIDGNDSNSNPLAE---------------------ADR--TKRVTFQSEG 420

Query: 421 VPENRRRRRLWEYLAEDDTQDGLDLSGSDRFAFSVDDALSVNS--AGSVAGRRWLLLSKL 480
                RRRRLWE LAE + +DGLDLSGS+RFAFSVDDAL+V++  +GS   RRWLLLSKL
Sbjct: 421 -----RRRRLWELLAEAEAEDGLDLSGSERFAFSVDDALTVSNEGSGSAVSRRWLLLSKL 480

Query: 481 RQGLSKHKEG--HVKTLFPWKYGLGDKEKADETENRVEETSYRSGKRRKEGLIDHELVLG 540
           +QGLSKHKEG  H + LFPW++GL DKE+  E EN VEE S  +G+RRKEG +DHELVL 
Sbjct: 481 KQGLSKHKEGQGHGRKLFPWRFGLEDKEEV-EVENTVEERSAPTGRRRKEGPVDHELVLS 540

Query: 541 ALSRALEITELAYLDMTDKLLDTNPELALMGSCLLVVLMRDEDVYVMNLGDSRAIVAQYE 600
           ALSRAL++TE AYL+MTDK+LDTNPELALMGSCLLVVLMRDEDVYVMNLGDSRAIVA YE
Sbjct: 541 ALSRALQVTENAYLEMTDKVLDTNPELALMGSCLLVVLMRDEDVYVMNLGDSRAIVAHYE 600

Query: 601 QQEIGPSEDMKEEGHKRTGMEGIIEESTTSEGKITPTNQPSAQTTRLTALQLSTDHSTSI 660
            +E G S  M+  GHK  G+    E+ T S   +   N+   Q  +LTALQLSTDHSTSI
Sbjct: 601 PEEGGSS--MESRGHKDNGLSA--EDITESSSAVA--NEAPTQAMKLTALQLSTDHSTSI 660

Query: 661 EEEVRRIKNEHPDDKQCIVNDRVKGRLKVTRAFGAGFLKQPKLNDALLEMFRNEYIGTAP 720
           EEEV RIKNEHPDDK CIVNDRVKGRLK            PK ND LLEMFRNEYIGT P
Sbjct: 661 EEEVIRIKNEHPDDKHCIVNDRVKGRLK------------PKWNDTLLEMFRNEYIGTTP 720

Query: 721 YLSCLPSLRHHRLCPTDQFIVLSSDGLYQYLTNQEVVSYVENFMEKFPDGDPAQHLIEEL 780
           Y+SCLPSLRHHRLCP+DQF++LSSDGLYQY +NQEVVS+VENFMEKFPDGDPAQHLIEEL
Sbjct: 721 YISCLPSLRHHRLCPSDQFLILSSDGLYQYFSNQEVVSHVENFMEKFPDGDPAQHLIEEL 765

Query: 781 LCRAARKAGMDFHELLDIPQGDRRKYHDDVTVMVISLEGRIWKSSGKYL 814
           L RAA+KAGMDFHELLDIPQGDRRKYHDDVTVMVISLEGRIWKSSGKYL
Sbjct: 781 LFRAAKKAGMDFHELLDIPQGDRRKYHDDVTVMVISLEGRIWKSSGKYL 765

BLAST of Cucsa.321690 vs. TAIR10
Match: AT2G35350.1 (AT2G35350.1 poltergeist like 1)

HSP 1 Score: 827.8 bits (2137), Expect = 5.9e-240
Identity = 469/832 (56.37%), Postives = 566/832 (68.03%), Query Frame = 1

Query: 1   MGSGVSSLFSCLKPETR-----PAALPADNSDILFSASDPLDETLGHSFCYV-RSSNRFL 60
           MGSG SSL  C     R      +A    +SD++ S  +PLDETLGHS+CYV  SSNRF+
Sbjct: 1   MGSGFSSLLPCFNQGHRNRRRHSSAANPSHSDLIDSFREPLDETLGHSYCYVPSSSNRFI 60

Query: 61  SPSHSDRFLSPSQSLRFSPPHDPSSLPTTTTRTRTGPPETAFKAISGASVSANSSIPRSV 120
           SP  SDRF+SP+ S R SPPH+P  +  + +  +     T F+AISGASVSAN+S  ++V
Sbjct: 61  SPFPSDRFVSPTASFRLSPPHEPGRIRGSGSSEQL---HTGFRAISGASVSANTSNSKTV 120

Query: 121 LMLDAVYDDATDTALGASAGGCGVRGSILN--GFESTSSFTALPLQPVPRGGTEPLERGG 180
           L L+ +YDDAT+++ G      GVR S++N  GFE TSSF+ALPLQP P       +R G
Sbjct: 121 LQLEDIYDDATESSFGG-----GVRRSVVNANGFEGTSSFSALPLQPGP-------DRSG 180

Query: 181 FFLSGPIERGALSGPLDANVDAAAAAAAAGGGSGGGRVHFSAPLGGMYVKKKRKKGISGF 240
            F+SGPIERGA SGPLD      + + +AG       VHFSAPLGG+Y KK+RKK     
Sbjct: 181 LFMSGPIERGATSGPLDPPAGEISRSNSAG-------VHFSAPLGGVYSKKRRKKKKKSL 240

Query: 241 R-KAFTRNFSDKRPWVVPVLNFV-GRKESSTAGDEPEVR----NESDVQWALGKAGEDRV 300
                      +RPWV+PV NFV G K+ +    + E       E+D+QWALGKAGEDRV
Sbjct: 241 SWHPIFGGEKKQRPWVLPVSNFVVGAKKENIVRPDVEAMAASSGENDLQWALGKAGEDRV 300

Query: 301 HVVVSEEQGWLFVGIYDGFNGPDAPEFLMGNLYRAVFNELQGLFWEIDDNEEAAANSNPP 360
            + V E+QGWLF GIYDGFNGPDAPEFLM NLYRAV +ELQGLFWE+++ ++   + +  
Sbjct: 301 QLAVFEKQGWLFAGIYDGFNGPDAPEFLMANLYRAVHSELQGLFWELEEEDDNPTDISTR 360

Query: 361 NVAGESTVGVRVNETNADPSSRPSSQTIQTNGNISVVGNEVEVEQPAADRGSAKRVTFQS 420
            +  +      VNE  +  SS P++              E E E+        KR+T  S
Sbjct: 361 ELEQQGEFEDHVNEMAS--SSCPAT--------------EKEEEE------MGKRLT-SS 420

Query: 421 EDVPENRRRRRLWEYLAEDDTQDGLDLSGSDRFAFSVDDALSVNSAGSVAGRRWLLLSKL 480
            +V E + R+RLWE LAE   +D LDLSGSDRFAFSVDDA+   +A SV  +RWLLLSKL
Sbjct: 421 LEVVEVKERKRLWELLAEAQAEDALDLSGSDRFAFSVDDAIGAGNAVSVGSKRWLLLSKL 480

Query: 481 RQGLSKHKEGHVKTLFPWKYGLGDKEKADETENRVEETSYRSGKRRKEGLIDHELVLGAL 540
           +QGLSK      K LFPWK G+ + E  +     VEE   +  KRRK G +DHELVL A+
Sbjct: 481 KQGLSKQGISGRK-LFPWKSGVEENETEEVDNVGVEEGVDKRRKRRKAGTVDHELVLKAM 540

Query: 541 SRALEITELAYLDMTDKLLDTNPELALMGSCLLVVLMRDEDVYVMNLGDSRAIVAQYEQQ 600
           S  LE TE A+L+MTDK+L+TNPELALMGSCLLV LMRD+DVY+MN+GDSRA+VAQY+ +
Sbjct: 541 SNGLEATEQAFLEMTDKVLETNPELALMGSCLLVALMRDDDVYIMNIGDSRALVAQYQVE 600

Query: 601 EIGPSEDMKEEGHKRTGM----EGIIEESTTSEGKITPTNQPSAQTTRLTALQLSTDHST 660
           E G S +  E   +R       +G  E         T  N+     T+L ALQL+TDHST
Sbjct: 601 ETGESVETAERVEERRNDLDRDDGNKEPLVVDSSDSTVNNEAPLPQTKLVALQLTTDHST 660

Query: 661 SIEEEVRRIKNEHPDDKQCIVNDRVKGRLKVTRAFGAGFLKQPKLNDALLEMFRNEYIGT 720
           SIE+EV RIKNEHPDD  CIVNDRVKGRLKVTRAFGAGFLKQPKLNDALLEMFRNEYIGT
Sbjct: 661 SIEDEVTRIKNEHPDDNHCIVNDRVKGRLKVTRAFGAGFLKQPKLNDALLEMFRNEYIGT 720

Query: 721 APYLSCLPSLRHHRLCPTDQFIVLSSDGLYQYLTNQEVVSYVENFMEKFPDGDPAQHLIE 780
            PY+SC PSLRH+RL   DQF+VLSSDGLYQYL+N EVVS     MEKFPDGDPAQH+I+
Sbjct: 721 DPYISCTPSLRHYRLTENDQFMVLSSDGLYQYLSNVEVVSLA---MEKFPDGDPAQHVIQ 780

Query: 781 ELLCRAARKAGMDFHELLDIPQGDRRKYHDDVTVMVISLEG-RIWKSSGKYL 814
           ELL RAA+KAGMDFHELLDIPQGDRRKYHDD TV+VI+L G RIWKSSGKYL
Sbjct: 781 ELLVRAAKKAGMDFHELLDIPQGDRRKYHDDCTVLVIALGGSRIWKSSGKYL 783

BLAST of Cucsa.321690 vs. TAIR10
Match: AT2G46920.1 (AT2G46920.1 Protein phosphatase 2C family protein)

HSP 1 Score: 570.1 bits (1468), Expect = 2.2e-162
Identity = 368/886 (41.53%), Postives = 508/886 (57.34%), Query Frame = 1

Query: 1   MGSGVSSLFSCLKPETRPAALPADNSDILFSASDPLDETLGHSFCYVRSS---NRFLSPS 60
           MG+G S +  C  P         D + +     +PLDE LGHSFCYVR S   +  ++PS
Sbjct: 1   MGNGTSRVVGCFVPSN-------DKNGVDLEFLEPLDEGLGHSFCYVRPSIFESPDITPS 60

Query: 61  HSDRFLSPSQ---------SLRFSPPHDPSSLPTTTTRTRTGPPETAFKAISGASVSANS 120
           +S+RF   S          S R     DPS L    ++   G  ET FKAISGASVSAN 
Sbjct: 61  NSERFTIDSSTIDSETLTGSFRNDIVDDPSFLNRHNSK---GLAETTFKAISGASVSANV 120

Query: 121 SIPRSVLMLDAVYDDATDTALGASAGGCGVRGSILNGFESTSSFTALPLQPVPRGGTEPL 180
           S  R+   +     D  + A                 FESTSSF ++PLQP+PRGG+ PL
Sbjct: 121 STARTGNQMALCSSDVLEPAAS---------------FESTSSFASIPLQPLPRGGSGPL 180

Query: 181 ERGGFFLSGPIERGALSGPLDANVDAAAAAAAAGGGSG----GGRVHFSAPLGGMYVKKK 240
                F+SGP+ERG  SGPLD N    +     G  SG      R +FSAPL   + +KK
Sbjct: 181 NG---FMSGPLERGFASGPLDRNNGFMSGPIEKGVMSGPLDVSDRSNFSAPLS--FRRKK 240

Query: 241 RK-----KGISG-FRKAFTRNFSDK--------RPWVVPV--LNFVGRKESSTAGDEPEV 300
            +     + +SG  +    R FS +        R ++ P   +++   K+    G++PE 
Sbjct: 241 PRFQRFMRSVSGPMKSTLARTFSRRSGGLSWMHRFFLHPETRVSWAVGKDGKLHGEDPES 300

Query: 301 RNESD--VQWALGKAGEDRVHVVVSEEQGWLFVGIYDGFNGPDAPEFLMGNLYRAVFNEL 360
             ES+  +QWA GKAGEDRVHVV+SEEQGWLF+GIYDGF+GPDAP+F+M +LY+A+  EL
Sbjct: 301 CLESNRNLQWAHGKAGEDRVHVVLSEEQGWLFIGIYDGFSGPDAPDFVMSHLYKAIDKEL 360

Query: 361 QGLFWEID---DNEEAAANSNPP---NVAGESTVGVRVNETNADPSSRPSSQTIQTNGNI 420
           +GL W+ +   ++ +   +  PP   N+    ++  + +++    S       I + GN 
Sbjct: 361 EGLLWDYEEPSEDNQLQPDQEPPTEENMCDPESISEQHSKSVVAESEEVMIDDISSLGN- 420

Query: 421 SVVGNEVEVEQPAADRGSAKR----------VTFQSEDVPENRRRRRLWEYLAEDDTQD- 480
                ++    P    G  K+            ++ E++   R    +      +  ++ 
Sbjct: 421 --TDTQIADGPPGDSAGPGKKSMRLYELLQLEQWEGEEIGLKRYGGNVALNNMTNQVENP 480

Query: 481 -------GLDLSGSDRFAFSVDDALSVNSAGSVAGRRWLLLSKLRQGLSKHKEGHVKTLF 540
                  G D   +DR A    D +  NS      ++  + SK+R+   K K    K LF
Sbjct: 481 STSGGGAGNDPCTTDRSAL---DGIP-NSGQRHGTKKSQISSKIRRMYQKQKSLR-KKLF 540

Query: 541 PWKYGLGDKEKADETENRVEETSYRSGKRRKEGLIDHELVLGALSRALEITELAYLDMTD 600
           PW Y    +E+    E ++ E+S    +RR  G +DH+ VL A++RALE TE AY+DM +
Sbjct: 541 PWSYD-WHREEGICVEEKIVESS-GPIRRRWSGTVDHDAVLRAMARALESTEEAYMDMVE 600

Query: 601 KLLDTNPELALMGSCLLVVLMRDEDVYVMNLGDSRAIVAQYEQQEIGPSEDMKEE---GH 660
           K LD NPELALMGSC+LV+LM+D+DVYVMN+GDSRAI+AQ    +   +     +   GH
Sbjct: 601 KSLDINPELALMGSCVLVMLMKDQDVYVMNVGDSRAILAQERLHDRHSNPGFGNDEGIGH 660

Query: 661 KRTGMEGI-------IEESTTSEGKITP------TNQPSAQTTRLTALQLSTDHSTSIEE 720
           K    E +       I E +    + TP          ++   ++ A+QLS+DHSTS+EE
Sbjct: 661 KSRSRESLVRIELDRISEESPIHNQATPISVSNKNRDVTSYRLKMRAVQLSSDHSTSVEE 720

Query: 721 EVRRIKNEHPDDKQCIVNDRVKGRLKVTRAFGAGFLKQPKLNDALLEMFRNEYIGTAPYL 780
           E+ RI++EHP+D Q I+ DRVKG+LKVTRAFGAGFLK+P  N+ALLEMF+ EYIGT PY+
Sbjct: 721 EIWRIRSEHPEDDQSILKDRVKGQLKVTRAFGAGFLKKPNFNEALLEMFQVEYIGTDPYI 780

Query: 781 SCLPSLRHHRLCPTDQFIVLSSDGLYQYLTNQEVVSYVENFMEKFPDGDPAQHLIEELLC 813
           +C P   HHRL  +D+F+VLSSDGLY+Y +N+EVV++V  F+E  P+GDPAQ+LI ELL 
Sbjct: 781 TCEPCTVHHRLTSSDRFMVLSSDGLYEYFSNEEVVAHVTWFIENVPEGDPAQYLIAELLS 840

BLAST of Cucsa.321690 vs. TAIR10
Match: AT1G07630.1 (AT1G07630.1 pol-like 5)

HSP 1 Score: 359.8 bits (922), Expect = 4.5e-99
Identity = 187/319 (58.62%), Postives = 239/319 (74.92%), Query Frame = 1

Query: 490 DKEKADETENRVEETSYRSGKRRKEGLIDHELVLGALSRALEITELAYLDMTDKLLDTNP 549
           D+E  D      E+ S RSG  R   L +H  VL ALS+AL  TE AYLD  DK+LD NP
Sbjct: 353 DRESQDLDRRLKEQISRRSGSDR---LTNHSEVLEALSQALRKTEEAYLDTADKMLDENP 412

Query: 550 ELALMGSCLLVVLMRDEDVYVMNLGDSRAIVAQYEQQEIGPSEDMKEEGHKRTGMEGIIE 609
           ELALMGSC+LV+LM+ ED+YVMN+GDSRA++ Q  + +   ++        R  +E I E
Sbjct: 413 ELALMGSCVLVMLMKGEDIYVMNVGDSRAVLGQKSEPDYWLAKI-------RQDLERINE 472

Query: 610 ESTTSEGKITPTNQPSAQTTRLTALQLSTDHSTSIEEEVRRIKNEHPDDKQCIVNDRVKG 669
           E+  ++ +    +Q S+    L+A QL+ DHST+IEEEV RI+NEHPDD   + N+RVKG
Sbjct: 473 ETMMNDLEGCEGDQ-SSLVPNLSAFQLTVDHSTNIEEEVERIRNEHPDDVTAVTNERVKG 532

Query: 670 RLKVTRAFGAGFLKQPKLNDALLEMFRNEYIGTAPYLSCLPSLRHHRLCPTDQFIVLSSD 729
            LKVTRAFGAGFLKQPK N+ALLEMF+ +Y+G +PY++CLPSL HHRL   D+F++LSSD
Sbjct: 533 SLKVTRAFGAGFLKQPKWNNALLEMFQIDYVGKSPYINCLPSLYHHRLGSKDRFLILSSD 592

Query: 730 GLYQYLTNQEVVSYVENFMEKFPDGDPAQHLIEELLCRAARKAGMDFHELLDIPQGDRRK 789
           GLYQY TN+E VS VE F+   P+GDPAQHL++ELL RAA+KAGMDFHELL+IPQG+RR+
Sbjct: 593 GLYQYFTNEEAVSEVELFITLQPEGDPAQHLVQELLFRAAKKAGMDFHELLEIPQGERRR 652

Query: 790 YHDDVTVMVISLEGRIWKS 809
           YHDDV+++VISLEGR+WKS
Sbjct: 653 YHDDVSIVVISLEGRMWKS 660

HSP 2 Score: 181.8 bits (460), Expect = 1.7e-45
Identity = 138/395 (34.94%), Postives = 187/395 (47.34%), Query Frame = 1

Query: 1   MGSGVSSLFSCLKPETRPAALPADNSDILFSASDPLDETLGHSFCYVRSSNRFLSPSHSD 60
           MG+GV+ L  C          P D S +L    DPLDE LGHSFCYVR            
Sbjct: 1   MGNGVTKLSICFTGGGGERLRPKDISVLL---PDPLDEGLGHSFCYVRP----------- 60

Query: 61  RFLSPSQSLRFSPPHDPSSLPTTTTRTRTGPPETAFKAISGASVSANSSIPRSVLMLDAV 120
                          DP+ + ++   +      T F+ ISGASVSAN++ P S  + D  
Sbjct: 61  ---------------DPTLISSSKVHSEEDTTTTTFRTISGASVSANTATPLSTSLYDP- 120

Query: 121 YDDATDTALGASAGGCGVRGSILNGFESTSSFTALPLQPVPRG------GTEPLERGGFF 180
           Y      A                 FEST+SF+++PLQP+P+       G+ P+ERG  F
Sbjct: 121 YGHIDRAA----------------AFESTTSFSSIPLQPIPKSSGPIVLGSGPIERG--F 180

Query: 181 LSGPIERGALSGPLDANVDAAAAAAAAGGGSGGGRVHFSAPLG-GMYVKKKRKKGISGFR 240
           LSGPIERG +SGPLD  V   +            +   S   G  + V  +++  +   R
Sbjct: 181 LSGPIERGFMSGPLD-RVGLFSGPLDKPNSDHHHQFQRSFSHGLALRVGSRKRSLVRILR 240

Query: 241 KAFTRNFSDKRPWVVPVLNFVGRKESSTAGDEPE----VRNES----------------- 300
           +A ++  S  +  +V  +  V  K+S   G   E    + NE+                 
Sbjct: 241 RAISKTMSRGQNSIVAPIKSV--KDSDNWGIRSEKSRNLHNENLTVNSLNFSSEVSLDDD 300

Query: 301 ------DVQWALGKAGEDRVHVVVSEEQGWLFVGIYDGFNGPDAPEFLMGNLYRAVFNEL 360
                 ++QWA GKAGEDRVHVVVSEE GWLFVGIYDGFNGPDAP++L+ +LY  V  EL
Sbjct: 301 VSLENQNLQWAQGKAGEDRVHVVVSEEHGWLFVGIYDGFNGPDAPDYLLSHLYPVVHREL 343

Query: 361 QGLFWEIDDNEEAAANSNPPNVAGESTVGVRVNET 362
           +GL W+ D N E+ +     +   ES      +ET
Sbjct: 361 KGLLWD-DSNVESKSQDLERSNGDESCSNQEKDET 343

BLAST of Cucsa.321690 vs. TAIR10
Match: AT5G02400.1 (AT5G02400.1 pol-like 2)

HSP 1 Score: 345.5 bits (885), Expect = 8.9e-95
Identity = 182/326 (55.83%), Postives = 233/326 (71.47%), Query Frame = 1

Query: 484 WKYGLGDKEKADETENRVEETSYRSGKRRKEGLIDHELVLGALSRALEITELAYLDMTDK 543
           W+    +K K+D   NR ++      K       +H+ VL AL +AL  TE AYL++ D+
Sbjct: 368 WEKKSNNKTKSD---NRCDQ------KGSNSTTTNHKDVLKALLQALRKTEDAYLELADQ 427

Query: 544 LLDTNPELALMGSCLLVVLMRDEDVYVMNLGDSRAIVAQYEQQEIGPSEDMKEEGHKRTG 603
           ++  NPELALMGSC+LV LM+ EDVYVMN+GDSRA+        +G   ++     ++  
Sbjct: 428 MVKENPELALMGSCVLVTLMKGEDVYVMNVGDSRAV--------LGRKPNLATGRKRQKE 487

Query: 604 MEGIIEESTTSEGKITPTNQPSAQTTRLTALQLSTDHSTSIEEEVRRIKNEHPDDKQCIV 663
           +E I E+S+  + +I       A    L  LQL+ +HST IEEEVRRIK EHPDD   + 
Sbjct: 488 LERIREDSSLEDKEILMNG---AMRNTLVPLQLNMEHSTRIEEEVRRIKKEHPDDDCAVE 547

Query: 664 NDRVKGRLKVTRAFGAGFLKQPKLNDALLEMFRNEYIGTAPYLSCLPSLRHHRLCPTDQF 723
           NDRVKG LKVTRAFGAGFLKQPK NDALLEMFR +YIGT+PY++C PSL HH+L   D+F
Sbjct: 548 NDRVKGYLKVTRAFGAGFLKQPKWNDALLEMFRIDYIGTSPYITCSPSLCHHKLTSRDKF 607

Query: 724 IVLSSDGLYQYLTNQEVVSYVENFMEKFPDGDPAQHLIEELLCRAARKAGMDFHELLDIP 783
           ++LSSDGLY+Y +NQE +  VE+F+  FP+GDPAQHLI+E+L RAA K GMDFHELL+IP
Sbjct: 608 LILSSDGLYEYFSNQEAIFEVESFISAFPEGDPAQHLIQEVLLRAANKFGMDFHELLEIP 667

Query: 784 QGDRRKYHDDVTVMVISLEGRIWKSS 810
           QGDRR+YHDDV+V+VISLEGRIW+SS
Sbjct: 668 QGDRRRYHDDVSVIVISLEGRIWRSS 673

HSP 2 Score: 151.8 bits (382), Expect = 1.9e-36
Identity = 127/373 (34.05%), Postives = 165/373 (44.24%), Query Frame = 1

Query: 1   MGSGVSSLFSCLKPETRPAALPADNSDILFSASDPLDETLGHSFCYVRSSNRFLSPSHSD 60
           MG+GV++L  C       A   +   D+       + + LGHSFCY+R            
Sbjct: 1   MGNGVTTLTGCCTGTL--AGEISRRYDVSL-----VHDGLGHSFCYIR------------ 60

Query: 61  RFLSPSQSLRFSPPHDPSSLPTTTTRTRTGP-PETAFKAISGASVSANSSIPRSVLMLDA 120
                       P      LP+  +  R+    ET F++ISGASVSAN S   S   L +
Sbjct: 61  ------------PDLPGVVLPSPESPLRSDHIQETTFRSISGASVSANPSTALSGA-LSS 120

Query: 121 VYDDATDTALGASAGGCGVRGSILNGFESTSSFTALPLQPVPRG--------------GT 180
             D    +A+ ASA            FES+ +F +LPLQPVPRG              G+
Sbjct: 121 DSDCPYSSAVSASA------------FESSGNFASLPLQPVPRGSTWQSGPIVNESGLGS 180

Query: 181 EPLERGGFFLSGPIERGALSGPLDANVDAAAAAAAAGGGSGGGRVHFSAPLGGMYVKKKR 240
            P ER   FLSGPIE G  SGP+++                            +  K K 
Sbjct: 181 APFERR--FLSGPIESGLYSGPIESTKKTEKEKPKK-----------------IRKKPKS 240

Query: 241 KKGISGFRKAFTRNFSDK-----RPWVVPVLNFVGRKESSTAGDEPEVR----NES---- 300
           KK    F+  F    S+      +  V+  +N     +S     EP +     NE+    
Sbjct: 241 KKNFLTFKTLFANLISNNNKPRLKKSVIEPINGSDSSDSGRLHHEPVITSSRSNENPKSD 300

Query: 301 --------------DVQWALGKAGEDRVHVVVSEEQGWLFVGIYDGFNGPDAPEFLMGNL 332
                         DVQWA GKAGEDRVHVVVSE+ GW+FVGIYDGF+GPDAP++L+ NL
Sbjct: 301 LEEEDEKQSMNSVLDVQWAQGKAGEDRVHVVVSEDNGWVFVGIYDGFSGPDAPDYLLNNL 310

BLAST of Cucsa.321690 vs. TAIR10
Match: AT2G28890.1 (AT2G28890.1 poltergeist like 4)

HSP 1 Score: 339.7 bits (870), Expect = 4.9e-93
Identity = 173/289 (59.86%), Postives = 219/289 (75.78%), Query Frame = 1

Query: 522 VLGALSRALEITELAYLDMTDKLLDTNPELALMGSCLLVVLMRDEDVYVMNLGDSRAIVA 581
           VL ALS+AL  TE AYL+  D +LD NPELALMGSC+LV+LM+ EDVY+MN+GDSRA++ 
Sbjct: 374 VLKALSQALRKTEEAYLENADMMLDENPELALMGSCVLVMLMKGEDVYLMNVGDSRAVLG 433

Query: 582 QYEQQE--IGPSEDMKEEGHKRTGMEGIIEESTTSEGKITPTNQPSAQTTRLTALQLSTD 641
           Q  + +  IG  +   E           I E T ++       + ++    L+A QL+ D
Sbjct: 434 QKAESDYWIGKIKQDLER----------INEETMNDFDGCGDGEGASLVPTLSAFQLTVD 493

Query: 642 HSTSIEEEVRRIKNEHPDDKQCIVNDRVKGRLKVTRAFGAGFLKQPKLNDALLEMFRNEY 701
           HST++EEEV RI+ EHPDD   + N+RVKG LKVTRAFGAGFLKQPK N+ALLEMF+ +Y
Sbjct: 494 HSTNVEEEVNRIRKEHPDDASAVSNERVKGSLKVTRAFGAGFLKQPKWNNALLEMFQIDY 553

Query: 702 IGTAPYLSCLPSLRHHRLCPTDQFIVLSSDGLYQYLTNQEVVSYVENFMEKFPDGDPAQH 761
            GT+PY++CLPSL HHRL   DQF++LSSDGLYQY TN+E VS VE F+   P+GDPAQH
Sbjct: 554 KGTSPYINCLPSLYHHRLGSKDQFLILSSDGLYQYFTNEEAVSEVELFITLQPEGDPAQH 613

Query: 762 LIEELLCRAARKAGMDFHELLDIPQGDRRKYHDDVTVMVISLEGRIWKS 809
           L++ELL RAA+KAGMDFHELL+IPQG+RR+YHDDV+++VISLEGR+WKS
Sbjct: 614 LVQELLFRAAKKAGMDFHELLEIPQGERRRYHDDVSIVVISLEGRMWKS 652

HSP 2 Score: 187.2 bits (474), Expect = 4.0e-47
Identity = 139/383 (36.29%), Postives = 185/383 (48.30%), Query Frame = 1

Query: 1   MGSGVSSLFSCLKPETRPAALPADNSDILFSASDPLDETLGHSFCYVRSSNRFLSPSHSD 60
           MG+G+  L  CL         P    ++     DPLDE LGHSFCYVR            
Sbjct: 1   MGNGIGKLSKCLTGGAGRNKKP----ELSILEPDPLDEGLGHSFCYVR------------ 60

Query: 61  RFLSPSQSLRFSPPHDPSSLPTTTTRTRTGPPETAFKAISGASVSANSSIPRSVLMLDAV 120
                           P     ++++  +    T F+ ISGASVSAN++ P S  + D  
Sbjct: 61  ----------------PDPTRVSSSKVHSEEETTTFRTISGASVSANTATPLSTSLYDP- 120

Query: 121 YDDATDTALGASAGGCGVRGSILNGFESTSSFTALPLQPVPRG------GTEPLERGGFF 180
           Y      A                 FEST+SF+++PLQP+PR       G+ PLERG  F
Sbjct: 121 YGHIDRAA----------------AFESTTSFSSIPLQPIPRSSGPIVPGSGPLERG--F 180

Query: 181 LSGPIERGALSGPLDANVDAAAAAAAAGGGSGGGRVHFSAPLGGMYVKKKRKKGISGFRK 240
           LSGPIERG +SGPL    D ++       GS   +  FS  L  + V  ++   +   R+
Sbjct: 181 LSGPIERGFMSGPL----DGSSGPIDGKTGSDQFQRSFSHGLANLRVGSRKGSLVRVLRR 240

Query: 241 AFTRNFSD------------KRP-WV----------------VPVLNFVGRKESSTAGDE 300
           A ++  +             K P WV                V  LNF    E S   D+
Sbjct: 241 AISKTITRGQNSIVAPIKPVKEPDWVFGSDKTRIHQIENNLTVNSLNF--SSEGSLLDDD 300

Query: 301 PEVRNESDVQWALGKAGEDRVHVVVSEEQGWLFVGIYDGFNGPDAPEFLMGNLYRAVFNE 349
             + ++ ++QWA GKAGEDRVHVVVSEE GWLFVGIYDGFNGPDAP++L+ +LY AV  E
Sbjct: 301 VSLESQ-NLQWAQGKAGEDRVHVVVSEEHGWLFVGIYDGFNGPDAPDYLLSHLYPAVHRE 323

BLAST of Cucsa.321690 vs. NCBI nr
Match: gi|778675099|ref|XP_011650351.1| (PREDICTED: protein phosphatase 2C 29 [Cucumis sativus])

HSP 1 Score: 1626.7 bits (4211), Expect = 0.0e+00
Identity = 813/813 (100.00%), Postives = 813/813 (100.00%), Query Frame = 1

Query: 1   MGSGVSSLFSCLKPETRPAALPADNSDILFSASDPLDETLGHSFCYVRSSNRFLSPSHSD 60
           MGSGVSSLFSCLKPETRPAALPADNSDILFSASDPLDETLGHSFCYVRSSNRFLSPSHSD
Sbjct: 1   MGSGVSSLFSCLKPETRPAALPADNSDILFSASDPLDETLGHSFCYVRSSNRFLSPSHSD 60

Query: 61  RFLSPSQSLRFSPPHDPSSLPTTTTRTRTGPPETAFKAISGASVSANSSIPRSVLMLDAV 120
           RFLSPSQSLRFSPPHDPSSLPTTTTRTRTGPPETAFKAISGASVSANSSIPRSVLMLDAV
Sbjct: 61  RFLSPSQSLRFSPPHDPSSLPTTTTRTRTGPPETAFKAISGASVSANSSIPRSVLMLDAV 120

Query: 121 YDDATDTALGASAGGCGVRGSILNGFESTSSFTALPLQPVPRGGTEPLERGGFFLSGPIE 180
           YDDATDTALGASAGGCGVRGSILNGFESTSSFTALPLQPVPRGGTEPLERGGFFLSGPIE
Sbjct: 121 YDDATDTALGASAGGCGVRGSILNGFESTSSFTALPLQPVPRGGTEPLERGGFFLSGPIE 180

Query: 181 RGALSGPLDANVDAAAAAAAAGGGSGGGRVHFSAPLGGMYVKKKRKKGISGFRKAFTRNF 240
           RGALSGPLDANVDAAAAAAAAGGGSGGGRVHFSAPLGGMYVKKKRKKGISGFRKAFTRNF
Sbjct: 181 RGALSGPLDANVDAAAAAAAAGGGSGGGRVHFSAPLGGMYVKKKRKKGISGFRKAFTRNF 240

Query: 241 SDKRPWVVPVLNFVGRKESSTAGDEPEVRNESDVQWALGKAGEDRVHVVVSEEQGWLFVG 300
           SDKRPWVVPVLNFVGRKESSTAGDEPEVRNESDVQWALGKAGEDRVHVVVSEEQGWLFVG
Sbjct: 241 SDKRPWVVPVLNFVGRKESSTAGDEPEVRNESDVQWALGKAGEDRVHVVVSEEQGWLFVG 300

Query: 301 IYDGFNGPDAPEFLMGNLYRAVFNELQGLFWEIDDNEEAAANSNPPNVAGESTVGVRVNE 360
           IYDGFNGPDAPEFLMGNLYRAVFNELQGLFWEIDDNEEAAANSNPPNVAGESTVGVRVNE
Sbjct: 301 IYDGFNGPDAPEFLMGNLYRAVFNELQGLFWEIDDNEEAAANSNPPNVAGESTVGVRVNE 360

Query: 361 TNADPSSRPSSQTIQTNGNISVVGNEVEVEQPAADRGSAKRVTFQSEDVPENRRRRRLWE 420
           TNADPSSRPSSQTIQTNGNISVVGNEVEVEQPAADRGSAKRVTFQSEDVPENRRRRRLWE
Sbjct: 361 TNADPSSRPSSQTIQTNGNISVVGNEVEVEQPAADRGSAKRVTFQSEDVPENRRRRRLWE 420

Query: 421 YLAEDDTQDGLDLSGSDRFAFSVDDALSVNSAGSVAGRRWLLLSKLRQGLSKHKEGHVKT 480
           YLAEDDTQDGLDLSGSDRFAFSVDDALSVNSAGSVAGRRWLLLSKLRQGLSKHKEGHVKT
Sbjct: 421 YLAEDDTQDGLDLSGSDRFAFSVDDALSVNSAGSVAGRRWLLLSKLRQGLSKHKEGHVKT 480

Query: 481 LFPWKYGLGDKEKADETENRVEETSYRSGKRRKEGLIDHELVLGALSRALEITELAYLDM 540
           LFPWKYGLGDKEKADETENRVEETSYRSGKRRKEGLIDHELVLGALSRALEITELAYLDM
Sbjct: 481 LFPWKYGLGDKEKADETENRVEETSYRSGKRRKEGLIDHELVLGALSRALEITELAYLDM 540

Query: 541 TDKLLDTNPELALMGSCLLVVLMRDEDVYVMNLGDSRAIVAQYEQQEIGPSEDMKEEGHK 600
           TDKLLDTNPELALMGSCLLVVLMRDEDVYVMNLGDSRAIVAQYEQQEIGPSEDMKEEGHK
Sbjct: 541 TDKLLDTNPELALMGSCLLVVLMRDEDVYVMNLGDSRAIVAQYEQQEIGPSEDMKEEGHK 600

Query: 601 RTGMEGIIEESTTSEGKITPTNQPSAQTTRLTALQLSTDHSTSIEEEVRRIKNEHPDDKQ 660
           RTGMEGIIEESTTSEGKITPTNQPSAQTTRLTALQLSTDHSTSIEEEVRRIKNEHPDDKQ
Sbjct: 601 RTGMEGIIEESTTSEGKITPTNQPSAQTTRLTALQLSTDHSTSIEEEVRRIKNEHPDDKQ 660

Query: 661 CIVNDRVKGRLKVTRAFGAGFLKQPKLNDALLEMFRNEYIGTAPYLSCLPSLRHHRLCPT 720
           CIVNDRVKGRLKVTRAFGAGFLKQPKLNDALLEMFRNEYIGTAPYLSCLPSLRHHRLCPT
Sbjct: 661 CIVNDRVKGRLKVTRAFGAGFLKQPKLNDALLEMFRNEYIGTAPYLSCLPSLRHHRLCPT 720

Query: 721 DQFIVLSSDGLYQYLTNQEVVSYVENFMEKFPDGDPAQHLIEELLCRAARKAGMDFHELL 780
           DQFIVLSSDGLYQYLTNQEVVSYVENFMEKFPDGDPAQHLIEELLCRAARKAGMDFHELL
Sbjct: 721 DQFIVLSSDGLYQYLTNQEVVSYVENFMEKFPDGDPAQHLIEELLCRAARKAGMDFHELL 780

Query: 781 DIPQGDRRKYHDDVTVMVISLEGRIWKSSGKYL 814
           DIPQGDRRKYHDDVTVMVISLEGRIWKSSGKYL
Sbjct: 781 DIPQGDRRKYHDDVTVMVISLEGRIWKSSGKYL 813

BLAST of Cucsa.321690 vs. NCBI nr
Match: gi|659095681|ref|XP_008448711.1| (PREDICTED: protein phosphatase 2C 29 [Cucumis melo])

HSP 1 Score: 1607.0 bits (4160), Expect = 0.0e+00
Identity = 804/813 (98.89%), Postives = 809/813 (99.51%), Query Frame = 1

Query: 1   MGSGVSSLFSCLKPETRPAALPADNSDILFSASDPLDETLGHSFCYVRSSNRFLSPSHSD 60
           MGSGVSSLFSCLKPETRPAALPADNSD+LFSASDPLDETLGHSFCYVRSSNRFLSPSHSD
Sbjct: 1   MGSGVSSLFSCLKPETRPAALPADNSDVLFSASDPLDETLGHSFCYVRSSNRFLSPSHSD 60

Query: 61  RFLSPSQSLRFSPPHDPSSLPTTTTRTRTGPPETAFKAISGASVSANSSIPRSVLMLDAV 120
           RFLSPSQSLRFSPPHDPSSLPTT TRTRTGPPETAFKAISGASVSANSSIPRSVLMLDAV
Sbjct: 61  RFLSPSQSLRFSPPHDPSSLPTT-TRTRTGPPETAFKAISGASVSANSSIPRSVLMLDAV 120

Query: 121 YDDATDTALGASAGGCGVRGSILNGFESTSSFTALPLQPVPRGGTEPLERGGFFLSGPIE 180
           YDDATDTALGASAGGCGVRGSILNGFESTSSFTALPLQPVPRGGTEPLERGGFFLSGPIE
Sbjct: 121 YDDATDTALGASAGGCGVRGSILNGFESTSSFTALPLQPVPRGGTEPLERGGFFLSGPIE 180

Query: 181 RGALSGPLDANVDAAAAAAAAGGGSGGGRVHFSAPLGGMYVKKKRKKGISGFRKAFTRNF 240
           RGALSGPLDANVDAAAAAAAAGGGSGGGRVHFSAPLGGMYVKKKRKKGISGFRKAFTRNF
Sbjct: 181 RGALSGPLDANVDAAAAAAAAGGGSGGGRVHFSAPLGGMYVKKKRKKGISGFRKAFTRNF 240

Query: 241 SDKRPWVVPVLNFVGRKESSTAGDEPEVRNESDVQWALGKAGEDRVHVVVSEEQGWLFVG 300
           SDKRPWVVPVLNFVGRKESSTAGDEPEVRNESDVQWALGKAGEDRVHVVVSEEQGWLFVG
Sbjct: 241 SDKRPWVVPVLNFVGRKESSTAGDEPEVRNESDVQWALGKAGEDRVHVVVSEEQGWLFVG 300

Query: 301 IYDGFNGPDAPEFLMGNLYRAVFNELQGLFWEIDDNEEAAANSNPPNVAGESTVGVRVNE 360
           IYDGFNGPDAPEFLMGNLYRAVFNELQGLFWEIDDNEEAAANSNPPNVAGESTVGVRVNE
Sbjct: 301 IYDGFNGPDAPEFLMGNLYRAVFNELQGLFWEIDDNEEAAANSNPPNVAGESTVGVRVNE 360

Query: 361 TNADPSSRPSSQTIQTNGNISVVGNEVEVEQPAADRGSAKRVTFQSEDVPENRRRRRLWE 420
           TNADPSSR S QTIQTNGNISVVGNEVEVEQPAADRGSAKRVTFQSEDVPENRRRRRLWE
Sbjct: 361 TNADPSSRASGQTIQTNGNISVVGNEVEVEQPAADRGSAKRVTFQSEDVPENRRRRRLWE 420

Query: 421 YLAEDDTQDGLDLSGSDRFAFSVDDALSVNSAGSVAGRRWLLLSKLRQGLSKHKEGHVKT 480
           YLAEDDTQDGLDLSGSDRFAFSVDDALSVNSAGSVAGRRWLLLSKL+QGLSKHKEGHVKT
Sbjct: 421 YLAEDDTQDGLDLSGSDRFAFSVDDALSVNSAGSVAGRRWLLLSKLKQGLSKHKEGHVKT 480

Query: 481 LFPWKYGLGDKEKADETENRVEETSYRSGKRRKEGLIDHELVLGALSRALEITELAYLDM 540
           LFPWKYGLGDKEKADETENRVEETSYRSGKRRKEGLIDHELVLGALSRALEITELAYLDM
Sbjct: 481 LFPWKYGLGDKEKADETENRVEETSYRSGKRRKEGLIDHELVLGALSRALEITELAYLDM 540

Query: 541 TDKLLDTNPELALMGSCLLVVLMRDEDVYVMNLGDSRAIVAQYEQQEIGPSEDMKEEGHK 600
           TDKLLDTNPELALMGSCLLVVLMRDEDVYVMNLGDSRAIVAQYEQQE+GPSEDMKEEGHK
Sbjct: 541 TDKLLDTNPELALMGSCLLVVLMRDEDVYVMNLGDSRAIVAQYEQQEVGPSEDMKEEGHK 600

Query: 601 RTGMEGIIEESTTSEGKITPTNQPSAQTTRLTALQLSTDHSTSIEEEVRRIKNEHPDDKQ 660
           +TGMEGIIEESTTSEGKIT TN+PSAQTTRLTALQLSTDHSTSIEEEVRRIKNEHPDDKQ
Sbjct: 601 KTGMEGIIEESTTSEGKITLTNRPSAQTTRLTALQLSTDHSTSIEEEVRRIKNEHPDDKQ 660

Query: 661 CIVNDRVKGRLKVTRAFGAGFLKQPKLNDALLEMFRNEYIGTAPYLSCLPSLRHHRLCPT 720
           CIVNDRVKGRLKVTRAFGAGFLKQPKLNDALLEMFRNEYIGTAPYLSCLPSLRHHRLCPT
Sbjct: 661 CIVNDRVKGRLKVTRAFGAGFLKQPKLNDALLEMFRNEYIGTAPYLSCLPSLRHHRLCPT 720

Query: 721 DQFIVLSSDGLYQYLTNQEVVSYVENFMEKFPDGDPAQHLIEELLCRAARKAGMDFHELL 780
           DQFIVLSSDGLYQYLTNQEVVSYVENFMEKFPDGDPAQHLIEELLCRAARKAGMDFHELL
Sbjct: 721 DQFIVLSSDGLYQYLTNQEVVSYVENFMEKFPDGDPAQHLIEELLCRAARKAGMDFHELL 780

Query: 781 DIPQGDRRKYHDDVTVMVISLEGRIWKSSGKYL 814
           DIPQGDRRKYHDDVTVMVISLEGRIWKSSGKYL
Sbjct: 781 DIPQGDRRKYHDDVTVMVISLEGRIWKSSGKYL 812

BLAST of Cucsa.321690 vs. NCBI nr
Match: gi|703129383|ref|XP_010104350.1| (Protein phosphatase 2C 29 [Morus notabilis])

HSP 1 Score: 1116.3 bits (2886), Expect = 0.0e+00
Identity = 593/837 (70.85%), Postives = 661/837 (78.97%), Query Frame = 1

Query: 1   MGSGVSSLFSCLKPETRPAALPA--------DNSDILFSASDPLDETLGHSFCYVRSSNR 60
           MG G+S++F C  P +R               N D++F++S+PLDETLGHSF YVRSS R
Sbjct: 1   MGGGLSTVFPCFTPASRSNHPDPHHDLHSHHQNQDLIFTSSEPLDETLGHSFVYVRSSAR 60

Query: 61  FLSPSHSDRFLSPSQSLRFSPPHDPSSLPTTTTRTRTGPPETAFKAISGASVSANSSIPR 120
           FLSP+ SDRF+SPSQSLRFSP H+P       +RTR GPPET F+ ISGASVSAN+S PR
Sbjct: 61  FLSPTQSDRFVSPSQSLRFSPSHEPG-----VSRTRAGPPETGFRTISGASVSANTSTPR 120

Query: 121 SVLMLDAVYDDATDTALGASA---GGCGVRGSILNGFESTSSFTALPLQPVPRGG----- 180
           +VL LD +YDDAT+T LG      GG GVRGSI+NGFESTSSF+ALPLQPVPRGG     
Sbjct: 121 TVLQLDNIYDDATETTLGCGGVGVGGGGVRGSIVNGFESTSSFSALPLQPVPRGGGGGGG 180

Query: 181 --TEPLERGGFFLSGPIERGALSGPLDANVDAAAAAAAAGGGSGGGRVHFSAPLGGMYVK 240
             + P+ERGGFFLSGPIERGALSGPLDAN       AA  G  G GRVHFSAPLGG YVK
Sbjct: 181 EPSGPMERGGFFLSGPIERGALSGPLDAN-------AAGAGCDGSGRVHFSAPLGGPYVK 240

Query: 241 KKRKKGISGFRKAFTRNFSDK-RPWVVPVLNFVGRKESSTAGDEPEVRNE-SDVQWALGK 300
           +KRKKGISG RKAF RNFS+K RPWVVPVLNFVGRKESS  GDE E R+E S+VQWALGK
Sbjct: 241 RKRKKGISGIRKAFYRNFSEKKRPWVVPVLNFVGRKESSGLGDESEARSETSNVQWALGK 300

Query: 301 AGEDRVHVVVSEEQGWLFVGIYDGFNGPDAPEFLMGNLYRAVFNELQGLFWEIDDNEEAA 360
           AGEDRVHVVVSEEQGWLFVGIYDGFNGPDAPEFLMGNLYRAV NELQGLFW +D+ EEA 
Sbjct: 301 AGEDRVHVVVSEEQGWLFVGIYDGFNGPDAPEFLMGNLYRAVHNELQGLFWVVDEAEEAT 360

Query: 361 ANSNPPNV-AGESTVGVRVNETNADPSSRPSSQTIQTNGNISVVGNEVEVEQPAADRGSA 420
             S+P NV A E+   V V ETN                      +EV  E         
Sbjct: 361 --SDPQNVVAEENENAVAVEETNQ---------------------SEVRTE--------- 420

Query: 421 KRVTFQSEDV--PENRRRRRLWEYLAEDDTQDGLDLSGSDRFAFSVDDALSVNSAGSVAG 480
           KRVTFQSE+   P   RRRRL ++LAEDD +DGLDLSGS RFAFSVDDAL V++ GS A 
Sbjct: 421 KRVTFQSEETERPAETRRRRLRDFLAEDDAEDGLDLSGSQRFAFSVDDALIVSNGGSAAS 480

Query: 481 RRWLLLSKLRQGLSKHKEGHVKTLFPWKYGLGDKEKAD-ETENRVEET-SYRSGKRRKEG 540
           RRWLLLSKL+QGLSKH+EGH +  FPW++GL DKEK + E ENRVEE  S R  +RRKEG
Sbjct: 481 RRWLLLSKLKQGLSKHREGHGRKFFPWRFGLEDKEKVEVEVENRVEEERSNRGSRRRKEG 540

Query: 541 LIDHELVLGALSRALEITELAYLDMTDKLLDTNPELALMGSCLLVVLMRDEDVYVMNLGD 600
            +DHELVLGALSRALE+TELAYLDMTDK+LDTNPELALMGSCLLVVLMRDEDVYVMN+GD
Sbjct: 541 PVDHELVLGALSRALEVTELAYLDMTDKVLDTNPELALMGSCLLVVLMRDEDVYVMNVGD 600

Query: 601 SRAIVAQYEQQEIGPSEDMKEEGHKRTGMEGIIEESTTS-EGKITPTNQPSAQTTRLTAL 660
           SRAIVAQYE +E+G S   + +G   +G EGI+EES  + E  +   N+  AQ  +LTAL
Sbjct: 601 SRAIVAQYEPEEVGSSAHSRGQGENGSGTEGIVEESLAAGERAMKLENETPAQAMKLTAL 660

Query: 661 QLSTDHSTSIEEEVRRIKNEHPDDKQCIVNDRVKGRLKVTRAFGAGFLKQPKLNDALLEM 720
           QLSTDHST+IEEE+ RIKNEHPDDK CIVNDRVKGRLKVTRAFGAGFLKQPK NDALLEM
Sbjct: 661 QLSTDHSTNIEEEIIRIKNEHPDDKNCIVNDRVKGRLKVTRAFGAGFLKQPKWNDALLEM 720

Query: 721 FRNEYIGTAPYLSCLPSLRHHRLCPTDQFIVLSSDGLYQYLTNQEVVSYVENFMEKFPDG 780
           FRNEYIGTAPY+SC PSLRHHRLCP DQF+VLSSDGLYQYLTN+EVVS+VENFMEKFP+G
Sbjct: 721 FRNEYIGTAPYISCSPSLRHHRLCPRDQFLVLSSDGLYQYLTNEEVVSHVENFMEKFPEG 780

Query: 781 DPAQHLIEELLCRAARKAGMDFHELLDIPQGDRRKYHDDVTVMVISLEGRIWKSSGK 812
           DPAQHLIEELL RAA+KAGMDFH+LLDIPQGDRRKYHDDVTVM+ISLEGRIWKSSGK
Sbjct: 781 DPAQHLIEELLFRAAKKAGMDFHDLLDIPQGDRRKYHDDVTVMIISLEGRIWKSSGK 793

BLAST of Cucsa.321690 vs. NCBI nr
Match: gi|1009107560|ref|XP_015880342.1| (PREDICTED: protein phosphatase 2C 29 [Ziziphus jujuba])

HSP 1 Score: 1116.3 bits (2886), Expect = 0.0e+00
Identity = 596/858 (69.46%), Postives = 661/858 (77.04%), Query Frame = 1

Query: 1   MGSGVSSLFSCLKPETRPA----------------ALPADNSDILFSASDPLDETLGHSF 60
           MG G+SS+F C     R                   L     D++F+AS+PLDETLGHSF
Sbjct: 1   MGGGLSSVFPCFTAVNRNNNNSNNNQSEHHLQHHNQLQQHQQDLIFAASEPLDETLGHSF 60

Query: 61  CYVRSSNRFLSPSHSDRFLSPSQSLRFSPPHDPSSLPTTTTRTRTGPPETAFKAISGASV 120
           CYV SS RF+SP+ SDRFLSPS SLRFSP H+P  +P    RTR+GPPET FK ISGASV
Sbjct: 61  CYVPSSARFVSPTQSDRFLSPSHSLRFSPSHEPVGVP----RTRSGPPETVFKTISGASV 120

Query: 121 SANSSIPRSVLMLDAVYDDATDTALGASAGGCGVRGSILNGFESTSSFTALPLQPVPRGG 180
           SAN+S PR+VL L+ +YDDATD+ LG   G CGVRGSI+NGFESTSSF+ALPLQPVPRGG
Sbjct: 121 SANTSTPRTVLQLENIYDDATDSTLGGGVG-CGVRGSIVNGFESTSSFSALPLQPVPRGG 180

Query: 181 TEP---LERGGFFLSGPIERGALSGPLDANVDAAAAAAAAGGGSGGGRVHFSAPLGGMYV 240
            EP   +ERGGFFLSGPIERGALSGPLDAN      AA   GG GGG VHFSAPLGG+YV
Sbjct: 181 GEPSGPMERGGFFLSGPIERGALSGPLDAN------AAGHDGGGGGGGVHFSAPLGGLYV 240

Query: 241 KKKRKKGISGFRKAFTRNFSDK-RPWVVPVLNFVGRKESSTAGDEPEVRNESDVQWALGK 300
           KKKRKKGISG RKAF RNFS+K RPWVVPVLNFVGRKE S AGDE EV++ES+VQWALGK
Sbjct: 241 KKKRKKGISGIRKAFYRNFSEKKRPWVVPVLNFVGRKEGSAAGDESEVKSESNVQWALGK 300

Query: 301 AGEDRVHVVVSEEQGWLFVGIYDGFNGPDAPEFLMGNLYRAVFNELQGLFWEIDDNEEAA 360
           AGEDRVHVVVSEEQGWLFVGIYDGFNGPDAPEFLMGNLYRAV+NELQGLFWE+D+  +  
Sbjct: 301 AGEDRVHVVVSEEQGWLFVGIYDGFNGPDAPEFLMGNLYRAVYNELQGLFWEVDEANDDH 360

Query: 361 ANSNPP-----NVAGESTVGVRVNETNADPSSRPSSQTIQTNGNISVVGNEVEVEQPAAD 420
               PP         ES V V   ETN     +  SQ +               +   A 
Sbjct: 361 PRPPPPPPPFVEAVAESAVIVE-KETNHPLEIKEDSQILPP------------ADDDRAG 420

Query: 421 RGSAKRVTFQSEDVPE---------------NRRRRRLWEYLAEDDTQDGLDLSGSDRFA 480
            G AKRVTFQSE                   N RRRRLWE+LAE + +DGLDLSGS+RFA
Sbjct: 421 NG-AKRVTFQSEGQTTTTTTTESEKTETETGNNRRRRLWEFLAEAEAEDGLDLSGSERFA 480

Query: 481 FSVDDALSVNSAGSVAGRRWLLLSKLRQGLSKHKE--GHVKTLFPWKYGLGDKEKADETE 540
           FSVDDALSV++ GS   RRWLLLSKL+QGLSKHKE  GH +  FPW++GL DKEK  E +
Sbjct: 481 FSVDDALSVSNGGSAVSRRWLLLSKLKQGLSKHKESAGHGRKFFPWRFGLEDKEKV-EVD 540

Query: 541 NRVEETSYRSGKRRKEGLIDHELVLGALSRALEITELAYLDMTDKLLDTNPELALMGSCL 600
           NR EETS ++G++RKEG +DHELVL ALSRALE+TELAYL+MTDK+LDTNPELALMGSCL
Sbjct: 541 NRAEETSAQTGRKRKEGPVDHELVLSALSRALEVTELAYLEMTDKVLDTNPELALMGSCL 600

Query: 601 LVVLMRDEDVYVMNLGDSRAIVAQYEQQEIGPSEDMK--EEGHKRTGMEGIIEE-STTSE 660
           LVVLMRDEDVYVMN+GDSRAIVA YE +E+G S + +   +     G+EGIIEE S   E
Sbjct: 601 LVVLMRDEDVYVMNVGDSRAIVAHYEPEEVGSSVESRGRRDNGSGGGVEGIIEEASAEGE 660

Query: 661 GKITPTNQPSAQTTRLTALQLSTDHSTSIEEEVRRIKNEHPDDKQCIVNDRVKGRLKVTR 720
             I   N+ S QT RLTALQLSTDHSTSIEEE+ RIKNEHPDD  CIVNDRVKGRLKVTR
Sbjct: 661 RAIKVENEASEQTMRLTALQLSTDHSTSIEEEIIRIKNEHPDDNHCIVNDRVKGRLKVTR 720

Query: 721 AFGAGFLKQPKLNDALLEMFRNEYIGTAPYLSCLPSLRHHRLCPTDQFIVLSSDGLYQYL 780
           AFGAGFLKQPK NDALLEMFRNEYIGTAPY+SCLPSLRHH+L P DQF+VLSSDGLYQYL
Sbjct: 721 AFGAGFLKQPKWNDALLEMFRNEYIGTAPYISCLPSLRHHKLYPRDQFLVLSSDGLYQYL 780

Query: 781 TNQEVVSYVENFMEKFPDGDPAQHLIEELLCRAARKAGMDFHELLDIPQGDRRKYHDDVT 814
           TN+EVVS VENF+EK PDGDPAQHLIEELL RAA+KAGMDFHELLDIPQGDRRKYHDDVT
Sbjct: 781 TNEEVVSQVENFLEKSPDGDPAQHLIEELLFRAAKKAGMDFHELLDIPQGDRRKYHDDVT 832

BLAST of Cucsa.321690 vs. NCBI nr
Match: gi|645265734|ref|XP_008238289.1| (PREDICTED: protein phosphatase 2C 29 [Prunus mume])

HSP 1 Score: 1095.9 bits (2833), Expect = 0.0e+00
Identity = 586/829 (70.69%), Postives = 654/829 (78.89%), Query Frame = 1

Query: 1   MGSGVSSLFSCLKPETRPAALPADNSDILFSASDPLDETLGHSFCYVRSSNRFLSPSHSD 60
           MGSGVSSL SC +P  R      D++D++F+AS+PLDETLGHSFCYVRSS RFLSP+ SD
Sbjct: 1   MGSGVSSLSSCFRPVHRTNHHHPDHNDVVFAASEPLDETLGHSFCYVRSSARFLSPTQSD 60

Query: 61  RFLSPSQSLRFSPPHDPSSLPTTTTRTRTGPPETAFKAISGASVSANSSIPRSVLMLDAV 120
           RF+SPS SLRFSP H+P +      RTR G  ET FKAISGASVSANSS PR+VL LD +
Sbjct: 61  RFISPSNSLRFSPSHEPGA------RTRPGLHETGFKAISGASVSANSSTPRTVLQLDNI 120

Query: 121 YDDATDTALGASAGGCGVRGSILNGFESTSSFTALPLQPVPRGGTE----PLERGGFFLS 180
           YDDATD+ LG   GG  VRGSI+NGFESTSSF+ALPLQPVPRGG      P+ER GFFLS
Sbjct: 121 YDDATDSVLGGFGGG--VRGSIVNGFESTSSFSALPLQPVPRGGERDPSGPMERAGFFLS 180

Query: 181 GPIERGALSGPLDANVDAAAAAAAAGGGSGGGRVHFSAPLGGMYVKKKRKKGISGFRKAF 240
           GPIERGALSGPLD N +A             G+VHFSAPLGG+YVKK+RKKGISG RKA 
Sbjct: 181 GPIERGALSGPLDPNPNAHGP---------DGQVHFSAPLGGLYVKKRRKKGISGIRKAL 240

Query: 241 TRNFSDK--RPWVVPVLNFVGRKESSTA-GDEPEVRNESDVQWALGKAGEDRVHVVVSEE 300
            RNFS+K  RPWVVPVLNFVGRKE     GDE E ++E++VQWALGKAGEDRVHVVVSEE
Sbjct: 241 YRNFSEKKQRPWVVPVLNFVGRKEGPPPPGDEAEPKSETNVQWALGKAGEDRVHVVVSEE 300

Query: 301 QGWLFVGIYDGFNGPDAPEFLMGNLYRAVFNELQGLFWEIDDNE-----EAAANSNPPNV 360
           QGWLFVGIYDGFNGPDAPEFLMGNLYRA +NELQGLFWE+D+++     E A N+NPPNV
Sbjct: 301 QGWLFVGIYDGFNGPDAPEFLMGNLYRAFYNELQGLFWEVDEDDQTPGAEEANNTNPPNV 360

Query: 361 AGESTVGVRVNETNADPSSRPSSQTIQTNGNISVVGNEVEVEQPAADRGSAKRVTFQSED 420
           A ES V V V     D +S P ++                     ADR   KRVTFQSE 
Sbjct: 361 ATESEVVVDVKIDGNDSNSNPLAE---------------------ADR--TKRVTFQSEG 420

Query: 421 VPENRRRRRLWEYLAEDDTQDGLDLSGSDRFAFSVDDALSVNS--AGSVAGRRWLLLSKL 480
                RRRRLWE LAE + +DGLDLSGS+RFAFSVDDAL+V++  +GS   RRWLLLSKL
Sbjct: 421 -----RRRRLWELLAEAEAEDGLDLSGSERFAFSVDDALTVSNEGSGSAVSRRWLLLSKL 480

Query: 481 RQGLSKHKEG--HVKTLFPWKYGLGDKEKADETENRVEETSYRSGKRRKEGLIDHELVLG 540
           +QGLSKHKEG  H + LFPW++GL DKE+  E EN VEE S  +G+RRKEG +DHELVL 
Sbjct: 481 KQGLSKHKEGQGHGRKLFPWRFGLEDKEEV-EVENTVEERSAPTGRRRKEGPVDHELVLS 540

Query: 541 ALSRALEITELAYLDMTDKLLDTNPELALMGSCLLVVLMRDEDVYVMNLGDSRAIVAQYE 600
           ALSRAL++TE AYL+MTDK+LDTNPELALMGSCLLVVLMRDEDVYVMNLGDSRAIVA YE
Sbjct: 541 ALSRALQVTENAYLEMTDKVLDTNPELALMGSCLLVVLMRDEDVYVMNLGDSRAIVAHYE 600

Query: 601 QQEIGPSEDMKEEGHKRTGMEGIIEESTTSEGKITPTNQPSAQTTRLTALQLSTDHSTSI 660
            +E G S  M+  GHK  G+    E+ T S   +   N+   Q  +LTALQLSTDHSTSI
Sbjct: 601 PEEGGSS--MESRGHKDNGLSA--EDITESSSAVA--NEAPTQAMKLTALQLSTDHSTSI 660

Query: 661 EEEVRRIKNEHPDDKQCIVNDRVKGRLKVTRAFGAGFLKQPKLNDALLEMFRNEYIGTAP 720
           EEEV RIKNEHPDDK CIVNDRVKGRLKVTRAFGAGFLKQPK ND LLEMFRNEYIGT P
Sbjct: 661 EEEVIRIKNEHPDDKHCIVNDRVKGRLKVTRAFGAGFLKQPKWNDTLLEMFRNEYIGTTP 720

Query: 721 YLSCLPSLRHHRLCPTDQFIVLSSDGLYQYLTNQEVVSYVENFMEKFPDGDPAQHLIEEL 780
           Y+SCLPSLRHHRLCP+DQF++LSSDGLYQY +NQEVVS+VENFMEKFPDGDPAQHLIEEL
Sbjct: 721 YISCLPSLRHHRLCPSDQFLILSSDGLYQYFSNQEVVSHVENFMEKFPDGDPAQHLIEEL 777

Query: 781 LCRAARKAGMDFHELLDIPQGDRRKYHDDVTVMVISLEGRIWKSSGKYL 814
           L RAA+KAGMDFHELLDIPQGDRRKYHDDVTVMVISLEGRIWKSSGKYL
Sbjct: 781 LFRAAKKAGMDFHELLDIPQGDRRKYHDDVTVMVISLEGRIWKSSGKYL 777