Cucsa.318590 (gene) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.318590
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	Inositol-tetrakisphosphate 1-kinase
Location	scaffold03076 : 133771 .. 134802 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTCTAATTCTCCTTCTTCAAATCGCCTTCGTGTAGGTTACGCTTTTCCCCCAAACAAAGAGCGCAATGTAATTCGGCCATCTCTAATCGACTACGCCAAGTTACATAGCGTCGACCTCGTTCGTATCGACCTCCAAACGCCGCTCCTCCACCAAGGTCCATTCCACTGCATCATTCACAAACTCTACGACGATGCTTGGGCAGAGAATCTCCAAGAATTCGCCTCCAAGAATCCTGACGTCGTCGTCGTAGATCGGCCAGACCTGATCTCTAAGCTCTACAACAGAGTCTCCATGCTCGATGTAGTGAGCCAGGTGAAGGTTTCGGACTCTGACGTAAAAATCGAGGTGCCGAAACAGATACTTGTGAAACAGGAGGATGAAGCAATCGATTCGATTATGAAATTAGAGTTGAAATTTCCAGTGATTGCTAAGCCGATGGAGTCAGATGGAAGTGCAAAATCGCACGAAATGTCTCTAGTCTTCAACCGACGAGGATTGAAGGATTTGAACAAGCCCGTACTTGTACAGGAATTTGTGAACCACGGCGGAGTAATGTTCAAAATCTACGTTGCAGGAGATCAATCGATGTGTGTTAAGCGGAAATCGCTGCCGGATGTTGAGGAGACAGAGGAAGAACTAGAGAAGAAGACGGAGGGCGCGATGAAGTTTTCGCAGATATCTAGAGCAGAGGAGAAGAGTGAGAAATGCAACGGGGAGGCGAAGAAGGAGGATGAGGAAGAGATTGAAATGCCGCCGGAGAAGATAGTGAGGGAGGTATCGAGAGGGTTGAAGGAAGCGATGGGGATTAGGCTGTTCAATTTTGACATGATAAGAGATAGAAATAATGGAAGGTATTATGTGATAGATATAAATTACTTACCAGGATTTGCAGTTCTGCCGGAGTACGAGCCGTTTCTGACGAAATTCTTCAAGGAAGTTAGGGAGAAGAAGGTGGATGTGGATGAGGTGGCGTCCGCCACTGAAAACGATAAACAAAACCCACGGTGCTGCAGCTGTTTCCATTGA

mRNA sequence

ATGGCTTCTAATTCTCCTTCTTCAAATCGCCTTCGTGTAGGTTACGCTTTTCCCCCAAACAAAGAGCGCAATGTAATTCGGCCATCTCTAATCGACTACGCCAAGTTACATAGCGTCGACCTCGTTCGTATCGACCTCCAAACGCCGCTCCTCCACCAAGGTCCATTCCACTGCATCATTCACAAACTCTACGACGATGCTTGGGCAGAGAATCTCCAAGAATTCGCCTCCAAGAATCCTGACGTCGTCGTCGTAGATCGGCCAGACCTGATCTCTAAGCTCTACAACAGAGTCTCCATGCTCGATGTAGTGAGCCAGGTGAAGGTTTCGGACTCTGACGTAAAAATCGAGGTGCCGAAACAGATACTTGTGAAACAGGAGGATGAAGCAATCGATTCGATTATGAAATTAGAGTTGAAATTTCCAGTGATTGCTAAGCCGATGGAGTCAGATGGAAGTGCAAAATCGCACGAAATGTCTCTAGTCTTCAACCGACGAGGATTGAAGGATTTGAACAAGCCCGTACTTGTACAGGAATTTGTGAACCACGGCGGAGTAATGTTCAAAATCTACGTTGCAGGAGATCAATCGATGTGTGTTAAGCGGAAATCGCTGCCGGATGTTGAGGAGACAGAGGAAGAACTAGAGAAGAAGACGGAGGGCGCGATGAAGTTTTCGCAGATATCTAGAGCAGAGGAGAAGAGTGAGAAATGCAACGGGGAGGCGAAGAAGGAGGATGAGGAAGAGATTGAAATGCCGCCGGAGAAGATAGTGAGGGAGGTATCGAGAGGGTTGAAGGAAGCGATGGGGATTAGGCTGTTCAATTTTGACATGATAAGAGATAGAAATAATGGAAGGTATTATGTGATAGATATAAATTACTTACCAGGATTTGCAGTTCTGCCGGAGTACGAGCCGTTTCTGACGAAATTCTTCAAGGAAGTTAGGGAGAAGAAGGTGGATGTGGATGAGGTGGCGTCCGCCACTGAAAACGATAAACAAAACCCACGGTGCTGCAGCTGTTTCCATTGA

Coding sequence (CDS)

ATGGCTTCTAATTCTCCTTCTTCAAATCGCCTTCGTGTAGGTTACGCTTTTCCCCCAAACAAAGAGCGCAATGTAATTCGGCCATCTCTAATCGACTACGCCAAGTTACATAGCGTCGACCTCGTTCGTATCGACCTCCAAACGCCGCTCCTCCACCAAGGTCCATTCCACTGCATCATTCACAAACTCTACGACGATGCTTGGGCAGAGAATCTCCAAGAATTCGCCTCCAAGAATCCTGACGTCGTCGTCGTAGATCGGCCAGACCTGATCTCTAAGCTCTACAACAGAGTCTCCATGCTCGATGTAGTGAGCCAGGTGAAGGTTTCGGACTCTGACGTAAAAATCGAGGTGCCGAAACAGATACTTGTGAAACAGGAGGATGAAGCAATCGATTCGATTATGAAATTAGAGTTGAAATTTCCAGTGATTGCTAAGCCGATGGAGTCAGATGGAAGTGCAAAATCGCACGAAATGTCTCTAGTCTTCAACCGACGAGGATTGAAGGATTTGAACAAGCCCGTACTTGTACAGGAATTTGTGAACCACGGCGGAGTAATGTTCAAAATCTACGTTGCAGGAGATCAATCGATGTGTGTTAAGCGGAAATCGCTGCCGGATGTTGAGGAGACAGAGGAAGAACTAGAGAAGAAGACGGAGGGCGCGATGAAGTTTTCGCAGATATCTAGAGCAGAGGAGAAGAGTGAGAAATGCAACGGGGAGGCGAAGAAGGAGGATGAGGAAGAGATTGAAATGCCGCCGGAGAAGATAGTGAGGGAGGTATCGAGAGGGTTGAAGGAAGCGATGGGGATTAGGCTGTTCAATTTTGACATGATAAGAGATAGAAATAATGGAAGGTATTATGTGATAGATATAAATTACTTACCAGGATTTGCAGTTCTGCCGGAGTACGAGCCGTTTCTGACGAAATTCTTCAAGGAAGTTAGGGAGAAGAAGGTGGATGTGGATGAGGTGGCGTCCGCCACTGAAAACGATAAACAAAACCCACGGTGCTGCAGCTGTTTCCATTGA

Protein sequence

MASNSPSSNRLRVGYAFPPNKERNVIRPSLIDYAKLHSVDLVRIDLQTPLLHQGPFHCIIHKLYDDAWAENLQEFASKNPDVVVVDRPDLISKLYNRVSMLDVVSQVKVSDSDVKIEVPKQILVKQEDEAIDSIMKLELKFPVIAKPMESDGSAKSHEMSLVFNRRGLKDLNKPVLVQEFVNHGGVMFKIYVAGDQSMCVKRKSLPDVEETEEELEKKTEGAMKFSQISRAEEKSEKCNGEAKKEDEEEIEMPPEKIVREVSRGLKEAMGIRLFNFDMIRDRNNGRYYVIDINYLPGFAVLPEYEPFLTKFFKEVREKKVDVDEVASATENDKQNPRCCSCFH*

Homology

[Close]

BLAST of Cucsa.318590 vs. Swiss-Prot
Match: ITPK1_ARATH (Inositol-tetrakisphosphate 1-kinase 1 OS=Arabidopsis thaliana GN=ITPK1 PE=1 SV=1)

HSP 1 Score: 292.7 bits (748), Expect = 5.1e-78
Identity = 148/313 (47.28%), Postives = 215/313 (68.69%), Query Frame = 1


Query: 10  RLRVGYAFPPNKERNVIRPSLIDYAKLHSVDLVRIDLQTPLLHQGPFHCIIHKLYDDAWA 69
           R  VGYA    K+ + I+PSLI++++   +DLV++D    LL QG   CIIHKLYD  W 
Sbjct: 8   RYLVGYALAAKKQHSFIQPSLIEHSRQRGIDLVKLDPTKSLLEQGKLDCIIHKLYDVYWK 67

Query: 70  ENLQEFASKNPDVVVVDRPDLISKLYNRVSMLDVVSQVK--VSDSDVKIEVPKQILVKQE 129
           ENL EF  K P V V+D P+ I +L+NRVSML+V++Q++  VSDS+ +  VP+Q++V   
Sbjct: 68  ENLHEFREKCPGVPVIDLPEAIERLHNRVSMLEVITQLRFPVSDSE-RFGVPEQVVVMDS 127

Query: 130 DEAIDSIMKLELKFPVIAKPMESDGSAKSHEMSLVFNRRGLKDLNKPVLVQEFVNHGGVM 189
                     ELKFPVIAKP+++DGSAKSH+M L++++ G+K L  P+++QEFVNHGGV+
Sbjct: 128 SVLSGGGALGELKFPVIAKPLDADGSAKSHKMFLIYDQEGMKILKAPIVLQEFVNHGGVI 187

Query: 190 FKIYVAGDQSMCVKRKSLPDVEETEEELEKKTEGAMKFSQISRAEEKSEKCNGEAKKEDE 249
           FK+YV GD   CVKR+SLPD+    EE    ++G++ FSQIS    + +K     +    
Sbjct: 188 FKVYVVGDHVKCVKRRSLPDI---SEEKIGTSKGSLPFSQISNLTAQEDKNIEYGEDRSL 247

Query: 250 EEIEMPPEKIVREVSRGLKEAMGIRLFNFDMIRD-RNNGRYYVIDINYLPGFAVLPEYEP 309
           E++EMPP   + ++++ ++E+MG+ LFNFD+IRD ++  RY +IDINY PG+A +P YEP
Sbjct: 248 EKVEMPPLSFLTDLAKAMRESMGLNLFNFDVIRDAKDANRYLIIDINYFPGYAKMPSYEP 307

Query: 310 FLTKFFKEVREKK 320
            LT+FF ++  KK
Sbjct: 308 VLTEFFWDMVTKK 316

[Close]

BLAST of Cucsa.318590 vs. Swiss-Prot
Match: ITPK1_MAIZE (Inositol-tetrakisphosphate 1-kinase 1 OS=Zea mays GN=ITPK1 PE=2 SV=1)

HSP 1 Score: 279.3 bits (713), Expect = 5.8e-74
Identity = 150/326 (46.01%), Postives = 207/326 (63.50%), Query Frame = 1


Query: 2   ASNSPSSN-----RLRVGYAFPPNKERNVIRPSLIDYAKLHSVDLVRIDLQTPLLHQGPF 61
           A+  PSS      R  +GYA  P K+++ I+PSL+  A    +DLV +D   PL  QGPF
Sbjct: 5   AAAEPSSGVTHPPRYVIGYALAPKKQQSFIQPSLVAQAASRGMDLVPVDASQPLAEQGPF 64

Query: 62  HCIIHKLYDDAWAENLQEFASKNPDVVVVDRPDLISKLYNRVSMLDVVSQVK-VSDSDVK 121
           H +IHKLY D W   L  FA+++P V +VD P  I +L+NR+SML VVS++   +D D  
Sbjct: 65  HLLIHKLYGDDWRAQLVAFAARHPAVPIVDPPHAIDRLHNRISMLQVVSELDHAADQDST 124

Query: 122 IEVPKQILVKQEDEAIDSIMKLELKFPVIAKPMESDGSAKSHEMSLVFNRRGLKDLNKPV 181
             +P Q++V       D  +   L+FP+IAKP+ +DG+AKSH+MSLV++R GL  L  P+
Sbjct: 125 FGIPSQVVVYDAAALADFGLLAALRFPLIAKPLVADGTAKSHKMSLVYHREGLGKLRPPL 184

Query: 182 LVQEFVNHGGVMFKIYVAGDQSMCVKRKSLPDVEETEEELEKKTEGAMKFSQISR--AEE 241
           ++QEFVNHGGV+FK+YV G    CVKR+SLPDV     E +   +G++ FSQ+S    E 
Sbjct: 185 VLQEFVNHGGVIFKVYVVGGHVTCVKRRSLPDV---SPEDDASAQGSVSFSQVSNLPTER 244

Query: 242 KSEKCNGEAKKEDEEEIEMPPEKIVREVSRGLKEAMGIRLFNFDMIRD-RNNGRYYVIDI 301
            +E+  GE   ED     +PP   + +++ GL+ A+G++LFNFDMIRD R   RY VIDI
Sbjct: 245 TAEEYYGEKSLED---AVVPPAAFINQIAGGLRRALGLQLFNFDMIRDVRAGDRYLVIDI 304

Query: 302 NYLPGFAVLPEYEPFLTKFFKEVREK 319
           NY PG+A +P YE  LT FF E+  K
Sbjct: 305 NYFPGYAKMPGYETVLTDFFWEMVHK 324

[Close]

BLAST of Cucsa.318590 vs. Swiss-Prot
Match: ITPK5_ORYSJ (Inositol-tetrakisphosphate 1-kinase 5 OS=Oryza sativa subsp. japonica GN=ITPK5 PE=2 SV=1)

HSP 1 Score: 268.1 bits (684), Expect = 1.3e-70
Identity = 143/318 (44.97%), Postives = 202/318 (63.52%), Query Frame = 1


Query: 10  RLRVGYAFPPNKERNVIRPSLIDYAKLHSVDLVRIDLQTPLLHQGPFHCIIHKLYDDAWA 69
           R  +GYA  P K+++ I+PSL+  A    +DLV +D   PL  QGPFH +IHKLY + W 
Sbjct: 15  RYLIGYALAPKKQQSFIQPSLVSRAAGRGMDLVPVDPSRPLPEQGPFHLLIHKLYGEEWR 74

Query: 70  ENLQEFASKNPDVVVVDRPDLISKLYNRVSMLDVVSQVKV---SDSDVKIEVPKQILVKQ 129
             L  F++ +P V VVD P  I +L+NR+SML VVS++ V   +       +P Q++V  
Sbjct: 75  GQLDAFSAAHPAVPVVDPPHAIDRLHNRISMLQVVSELDVPLHAHHHHTFGIPSQVVVYD 134

Query: 130 EDEAIDSIMKLELKFPVIAKPMESDGSAKSHEMSLVFNRRGLKDLNKPVLVQEFVNHGGV 189
                DS +   L+FP+IAKP+ +DG+AKSH+MSLV++R GL+ L  P+++QEFVNHGGV
Sbjct: 135 AAALSDSGLLAALRFPLIAKPLVADGTAKSHKMSLVYHREGLRKLRPPLVLQEFVNHGGV 194

Query: 190 MFKIYVAGDQSMCVKRKSLPDVEETEEELEKKTEGAMKFSQISRA--EEKSEKCNGEAKK 249
           +FK+YV G    CVKR+SLPDV  ++   +   EG++ FSQ+S    E  +++   + + 
Sbjct: 195 IFKVYVVGAHVTCVKRRSLPDV-SSDVLQDASAEGSLSFSQVSNLPNERTAQEYYDDMRL 254

Query: 250 EDEEEIEMPPEKIVREVSRGLKEAMGIRLFNFDMIRDRNNG-RYYVIDINYLPGFAVLPE 309
           ED     MPP   + +++  L+ A+G+ LFNFDMIRD   G RY VIDINY PG+A +P 
Sbjct: 255 ED---AIMPPTAFINDIAAALRRALGLHLFNFDMIRDARAGDRYLVIDINYFPGYAKMPG 314

Query: 310 YEPFLTKFFKEVREKKVD 322
           YE  LT FF E+  K  D
Sbjct: 315 YETVLTDFFWEMVHKDDD 328

[Close]

BLAST of Cucsa.318590 vs. Swiss-Prot
Match: ITPK5_ORYSI (Inositol-tetrakisphosphate 1-kinase 5 OS=Oryza sativa subsp. indica GN=ITPK5 PE=3 SV=1)

HSP 1 Score: 268.1 bits (684), Expect = 1.3e-70
Identity = 143/318 (44.97%), Postives = 202/318 (63.52%), Query Frame = 1


Query: 10  RLRVGYAFPPNKERNVIRPSLIDYAKLHSVDLVRIDLQTPLLHQGPFHCIIHKLYDDAWA 69
           R  +GYA  P K+++ I+PSL+  A    +DLV +D   PL  QGPFH +IHKLY + W 
Sbjct: 15  RYLIGYALAPKKQQSFIQPSLVSRAAGRGMDLVPVDPSRPLPEQGPFHLLIHKLYGEEWR 74

Query: 70  ENLQEFASKNPDVVVVDRPDLISKLYNRVSMLDVVSQVKV---SDSDVKIEVPKQILVKQ 129
             L  F++ +P V VVD P  I +L+NR+SML VVS++ V   +       +P Q++V  
Sbjct: 75  GQLDAFSAAHPAVPVVDPPHAIDRLHNRISMLQVVSELDVPLHAHHHHTFGIPSQVVVYD 134

Query: 130 EDEAIDSIMKLELKFPVIAKPMESDGSAKSHEMSLVFNRRGLKDLNKPVLVQEFVNHGGV 189
                DS +   L+FP+IAKP+ +DG+AKSH+MSLV++R GL+ L  P+++QEFVNHGGV
Sbjct: 135 AAALSDSGLLAALRFPLIAKPLVADGTAKSHKMSLVYHREGLRKLRPPLVLQEFVNHGGV 194

Query: 190 MFKIYVAGDQSMCVKRKSLPDVEETEEELEKKTEGAMKFSQISRA--EEKSEKCNGEAKK 249
           +FK+YV G    CVKR+SLPDV  ++   +   EG++ FSQ+S    E  +++   + + 
Sbjct: 195 IFKVYVVGAHVTCVKRRSLPDV-SSDVLQDASAEGSLSFSQVSNLPNERTAQEYYDDMRL 254

Query: 250 EDEEEIEMPPEKIVREVSRGLKEAMGIRLFNFDMIRDRNNG-RYYVIDINYLPGFAVLPE 309
           ED     MPP   + +++  L+ A+G+ LFNFDMIRD   G RY VIDINY PG+A +P 
Sbjct: 255 ED---AIMPPTAFINDIAAALRRALGLHLFNFDMIRDARAGDRYLVIDINYFPGYAKMPG 314

Query: 310 YEPFLTKFFKEVREKKVD 322
           YE  LT FF E+  K  D
Sbjct: 315 YETVLTDFFWEMVHKDDD 328

[Close]

BLAST of Cucsa.318590 vs. Swiss-Prot
Match: ITPK4_ORYSJ (Inositol-tetrakisphosphate 1-kinase 4 OS=Oryza sativa subsp. japonica GN=ITPK4 PE=2 SV=1)

HSP 1 Score: 252.7 bits (644), Expect = 5.9e-66
Identity = 143/319 (44.83%), Postives = 199/319 (62.38%), Query Frame = 1


Query: 4   NSPSSN-RLRVGYAFPPNKERNVIRPSLIDYAKLHSVDLVRIDLQTPLLHQGPFHCIIHK 63
           +SPSS+ R  VGYA  P K  +V+RPSL+  A    V LV +D+  PL  QGPF  ++HK
Sbjct: 6   SSPSSSPRYTVGYALLPEKVSSVVRPSLVALAADRGVRLVAVDVSRPLAEQGPFDLLVHK 65

Query: 64  LYDDAWAENLQEFASKNPDV-VVVDRPDLISKLYNRVSMLDVVSQVKVSDSDVKIEVPKQ 123
           +YD  W   L+E A+++P V VVVD P  I +L +R +MLDVVS ++       + VP Q
Sbjct: 66  MYDRGWRAQLEELAARHPGVTVVVDSPGAIDRLLDRATMLDVVSGLR-----TPVSVPPQ 125

Query: 124 ILVKQEDEAIDSIM-KLELKFPVIAKPMESDGSAKSHEMSLVFNRRG-LKDLNKPVLVQE 183
           ++V       D ++ +  L+FP+IAKP+  DGSA+SH+M LV+ R G L  L  P+++QE
Sbjct: 126 VVVSDAAADADELLARAALRFPLIAKPLAVDGSAESHDMRLVYRRDGVLPLLRAPLVLQE 185

Query: 184 FVNHGGVMFKIYVAGDQSMCVKRKSLPDVEETEEELEKKTEGAMKFSQISRAEEKSEKCN 243
           FVNHGGV+FK+YV GD++ CV+R SLPDV      L+   E ++ F+ IS         +
Sbjct: 186 FVNHGGVLFKVYVVGDRATCVRRSSLPDV-PARRLLDLDAEPSVPFANISNQPLPPPDDD 245

Query: 244 GEAKKEDEEEIEMPPEKIVREVSRGLKEAMGIRLFNFDMIRDR---NNGRYYVIDINYLP 303
           G A  +D      P    V EV+RGL+  +G+ LFNFDMIR+R   +  RY++IDINY P
Sbjct: 246 GGAADDD-----TPAAGFVDEVARGLRRGLGLHLFNFDMIRERSEEHGDRYFIIDINYFP 305

Query: 304 GFAVLPEYEPFLTKFFKEV 316
           G+A +P YE  LT FF E+
Sbjct: 306 GYAKMPGYEAALTDFFLEM 313

[Close]

BLAST of Cucsa.318590 vs. TrEMBL
Match: A0A0A0LRI5_CUCSA (Inositol-tetrakisphosphate 1-kinase OS=Cucumis sativus GN=Csa_2G439740 PE=3 SV=1)

HSP 1 Score: 686.8 bits (1771), Expect = 1.4e-194
Identity = 343/343 (100.00%), Postives = 343/343 (100.00%), Query Frame = 1


Query: 1   MASNSPSSNRLRVGYAFPPNKERNVIRPSLIDYAKLHSVDLVRIDLQTPLLHQGPFHCII 60
           MASNSPSSNRLRVGYAFPPNKERNVIRPSLIDYAKLHSVDLVRIDLQTPLLHQGPFHCII
Sbjct: 1   MASNSPSSNRLRVGYAFPPNKERNVIRPSLIDYAKLHSVDLVRIDLQTPLLHQGPFHCII 60

Query: 61  HKLYDDAWAENLQEFASKNPDVVVVDRPDLISKLYNRVSMLDVVSQVKVSDSDVKIEVPK 120
           HKLYDDAWAENLQEFASKNPDVVVVDRPDLISKLYNRVSMLDVVSQVKVSDSDVKIEVPK
Sbjct: 61  HKLYDDAWAENLQEFASKNPDVVVVDRPDLISKLYNRVSMLDVVSQVKVSDSDVKIEVPK 120

Query: 121 QILVKQEDEAIDSIMKLELKFPVIAKPMESDGSAKSHEMSLVFNRRGLKDLNKPVLVQEF 180
           QILVKQEDEAIDSIMKLELKFPVIAKPMESDGSAKSHEMSLVFNRRGLKDLNKPVLVQEF
Sbjct: 121 QILVKQEDEAIDSIMKLELKFPVIAKPMESDGSAKSHEMSLVFNRRGLKDLNKPVLVQEF 180

Query: 181 VNHGGVMFKIYVAGDQSMCVKRKSLPDVEETEEELEKKTEGAMKFSQISRAEEKSEKCNG 240
           VNHGGVMFKIYVAGDQSMCVKRKSLPDVEETEEELEKKTEGAMKFSQISRAEEKSEKCNG
Sbjct: 181 VNHGGVMFKIYVAGDQSMCVKRKSLPDVEETEEELEKKTEGAMKFSQISRAEEKSEKCNG 240

Query: 241 EAKKEDEEEIEMPPEKIVREVSRGLKEAMGIRLFNFDMIRDRNNGRYYVIDINYLPGFAV 300
           EAKKEDEEEIEMPPEKIVREVSRGLKEAMGIRLFNFDMIRDRNNGRYYVIDINYLPGFAV
Sbjct: 241 EAKKEDEEEIEMPPEKIVREVSRGLKEAMGIRLFNFDMIRDRNNGRYYVIDINYLPGFAV 300

Query: 301 LPEYEPFLTKFFKEVREKKVDVDEVASATENDKQNPRCCSCFH 344
           LPEYEPFLTKFFKEVREKKVDVDEVASATENDKQNPRCCSCFH
Sbjct: 301 LPEYEPFLTKFFKEVREKKVDVDEVASATENDKQNPRCCSCFH 343

[Close]

BLAST of Cucsa.318590 vs. TrEMBL
Match: A0A0A0LTT9_CUCSA (Inositol-tetrakisphosphate 1-kinase OS=Cucumis sativus GN=Csa_2G439240 PE=3 SV=1)

HSP 1 Score: 341.3 bits (874), Expect = 1.4e-90
Identity = 180/337 (53.41%), Postives = 230/337 (68.25%), Query Frame = 1


Query: 7   SSNRLRVGYAFPPNKERNVIRPSLIDYAKLHSVDLVRIDLQTPLLHQGPFHCIIHKLYDD 66
           SS R R+GYA    KER+ I PSL+DYAKLH +D VRID   PL  Q PFHCIIHKLYD 
Sbjct: 2   SSPRFRIGYALSLKKERSFILPSLVDYAKLHGIDFVRIDPLLPLTDQTPFHCIIHKLYDP 61

Query: 67  AWAENLQEFASKNPDVVVVDRPDLISKLYNRVSMLDVVSQVKVSDSDVKIEVPKQILVKQ 126
           +W + LQ+F S+ PDVVVVD P+LIS+L NR SML+VV +VKV   D +IE PKQ++V  
Sbjct: 62  SWVQQLQDFTSQFPDVVVVDPPELISRLLNRDSMLEVVKEVKVPQGDERIETPKQVVVND 121

Query: 127 ED----EAIDSIMKLELKFPVIAKPMESDGSAKSHEMSLVFNRRGLKDLNKPVLVQEFVN 186
            D      +++   L LKFP+IAKP+ES+GSAKSH++ LV N  GLK LN P+++QEFVN
Sbjct: 122 LDVVLKNGLNTFSDLGLKFPIIAKPLESNGSAKSHQLCLVSNDSGLKGLNAPIVLQEFVN 181

Query: 187 HGGVMFKIYVAGDQSMCVKRKSLPDVEETEEELEKKTEGAMKFSQISRAEEKSEKCNGEA 246
           HGGV+FK+YV G+  +CV RKSLPD+     E  KK +    FSQIS         N  A
Sbjct: 182 HGGVVFKVYVVGECVVCVTRKSLPDI---GPEDVKKLDAVSSFSQIS---------NSGA 241

Query: 247 KKEDEEEIEMPPEKIVREVSRGLKEAMGIRLFNFDMIRDRN-NGRYYVIDINYLPGFAVL 306
           + +DE  +EMP  + V  V+ GL+EA G+RLFNFD+IRD N + RY VIDINYLPG+A +
Sbjct: 242 QGDDEGNVEMPSLEFVMHVAAGLREATGLRLFNFDLIRDSNDHNRYLVIDINYLPGYAKM 301

Query: 307 PEYEPFLTKFFKEVREKKVDVDEVASATENDKQNPRC 339
           P YEPFLTKFF +V + +      +S+  N+ +   C
Sbjct: 302 PNYEPFLTKFFLDVVQNRTVGGAFSSSYVNENEVVFC 326

[Close]

BLAST of Cucsa.318590 vs. TrEMBL
Match: F6HEE3_VITVI (Inositol-tetrakisphosphate 1-kinase OS=Vitis vinifera GN=VIT_16s0039g01700 PE=3 SV=1)

HSP 1 Score: 317.4 bits (812), Expect = 2.2e-83
Identity = 163/315 (51.75%), Postives = 214/315 (67.94%), Query Frame = 1


Query: 10  RLRVGYAFPPNKERNVIRPSLIDYAKLHSVDLVRIDLQTPLLHQGPFHCIIHKLYDDAWA 69
           R  VGYA  P K  + I+PSL+D+AK   +DLVRIDL  PL+ QGPF CIIHK+ D+ W 
Sbjct: 7   RFTVGYALAPKKVSSFIQPSLVDHAKQRGIDLVRIDLDKPLIEQGPFDCIIHKMNDEDWK 66

Query: 70  ENLQEFASKNPDVVVVDRPDLISKLYNRVSMLDVVSQVKVSDSDVKIEVPKQILVKQEDE 129
             L+EF++KNP+VV++D PD I +L++R+SML+VV ++K+ +      +PKQI++   + 
Sbjct: 67  NQLEEFSTKNPNVVIIDPPDAIEQLHSRISMLEVVKELKIPEGTESFGIPKQIVIYDPES 126

Query: 130 AIDSIMKLELKFPVIAKPMESDGSAKSHEMSLVFNRRGLKDLNKPVLVQEFVNHGGVMFK 189
            +DS +   L FPVIAKP+ +DGSAKSH+MSL FN  GLK L  P+++QEFVNHGGV+FK
Sbjct: 127 LLDSKVLDGLSFPVIAKPLVADGSAKSHKMSLAFNGEGLKKLTTPIVLQEFVNHGGVIFK 186

Query: 190 IYVAGDQSMCVKRKSLPDVEETEEELEKKTEGAMKFSQISRAEEKSEKCNGEAKKED--- 249
           +YV GD   CVKR+SLPDV     E    +EG + FSQIS      E   GE   ED   
Sbjct: 187 VYVVGDHVTCVKRRSLPDV---SVEKLGTSEGLLTFSQISNLTATQEP--GENDCEDIMN 246

Query: 250 -EEEIEMPPEKIVREVSRGLKEAMGIRLFNFDMIRDRNNG-RYYVIDINYLPGFAVLPEY 309
             EE EMPP   V E++ GL++AMG+ LFNFD+IRD   G RY VIDINY PG+A +P Y
Sbjct: 247 HVEEAEMPPLNFVNEIAIGLRKAMGLNLFNFDVIRDAKLGNRYLVIDINYFPGYAKMPSY 306

Query: 310 EPFLTKFFKEVREKK 320
           E  LT FF ++  +K
Sbjct: 307 ETVLTDFFWDIVHRK 316

[Close]

BLAST of Cucsa.318590 vs. TrEMBL
Match: A5BKJ6_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_018923 PE=4 SV=1)

HSP 1 Score: 314.3 bits (804), Expect = 1.8e-82
Identity = 163/315 (51.75%), Postives = 212/315 (67.30%), Query Frame = 1


Query: 10  RLRVGYAFPPNKERNVIRPSLIDYAKLHSVDLVRIDLQTPLLHQGPFHCIIHKLYDDAWA 69
           R  VGYA  P K  + I+PSL+D+AK   +DLVRIDL  PL+ QGPF CIIHK+ D+ W 
Sbjct: 68  RFTVGYALAPKKVSSFIQPSLVDHAKQRGIDLVRIDLDKPLIGQGPFDCIIHKMNDEDWK 127

Query: 70  ENLQEFASKNPDVVVVDRPDLISKLYNRVSMLDVVSQVKVSDSDVKIEVPKQILVKQEDE 129
             L+EF++KNP+VV++D PD I +L++R+SML+VV  +K+ +      +PKQI++   + 
Sbjct: 128 NQLEEFSTKNPNVVIIDPPDAIEQLHSRISMLEVVKDLKILEGTESFGIPKQIVIYDPES 187

Query: 130 AIDSIMKLELKFPVIAKPMESDGSAKSHEMSLVFNRRGLKDLNKPVLVQEFVNHGGVMFK 189
            +DS +   L FPVIAKP+ +DGSAKSH+MSL FN  GLK L  P+++QEFVNHGGV+FK
Sbjct: 188 LLDSKVLDGLSFPVIAKPLVADGSAKSHKMSLAFNGEGLKKLTTPIVLQEFVNHGGVIFK 247

Query: 190 IYVAGDQSMCVKRKSLPDVEETEEELEKKTEGAMKFSQISRAEEKSEKCNGEAKKED--- 249
           +YV GD   CVKR+SLPDV     E    +EG + FSQIS      E   GE   ED   
Sbjct: 248 VYVVGDHVTCVKRRSLPDV---SXEKLGTSEGLLTFSQISNLTATQEP--GENDCEDIMN 307

Query: 250 -EEEIEMPPEKIVREVSRGLKEAMGIRLFNFDMIRDRNNG-RYYVIDINYLPGFAVLPEY 309
             EE EMPP   V E++ GL++AMG+ LFNFD+IRD   G RY VIDINY PG A +P Y
Sbjct: 308 HVEEAEMPPLNFVNEIAIGLRKAMGLNLFNFDVIRDAKLGNRYLVIDINYFPGXAKMPSY 367

Query: 310 EPFLTKFFKEVREKK 320
           E  LT FF ++  +K
Sbjct: 368 ETVLTDFFWDIVHRK 377

[Close]

BLAST of Cucsa.318590 vs. TrEMBL
Match: W9R373_9ROSA (Inositol-tetrakisphosphate 1-kinase OS=Morus notabilis GN=L484_017781 PE=3 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 2.4e-82
Identity = 156/334 (46.71%), Postives = 219/334 (65.57%), Query Frame = 1


Query: 7   SSNRLRVGYAFPPNKERNVIRPSLIDYAKLHSVDLVRIDLQTPLLHQGPFHCIIHKLYDD 66
           +  R  VGYA  P K+++ I+ SL+  A+   +DLVRI+ + PL  QGPF C++HKLY D
Sbjct: 2   AERRFGVGYALAPKKQQSFIQDSLVSLARSRGIDLVRIEREIPLADQGPFDCVLHKLYGD 61

Query: 67  AWAENLQEFASKNPDVVVVDRPDLISKLYNRVSMLDVVSQVKVSDSDVKIEVPKQILVKQ 126
            W   L++F +KNP+ V++D P+ I +L+NR+SML VVS++K+ D      +PKQI++  
Sbjct: 62  DWRRQLEDFRAKNPNAVIIDYPEAIERLHNRISMLQVVSELKIEDRSETFGIPKQIVIYD 121

Query: 127 EDEAIDSIMKLELKFPVIAKPMESDGSAKSHEMSLVFNRRGLKDLNKPVLVQEFVNHGGV 186
           ++   D      LKFPVIAKP+ +DGSAKSH+M+L+FN  GL +L  P+++QEFVNHGGV
Sbjct: 122 KETLFDRPAWEGLKFPVIAKPLVADGSAKSHKMALIFNHDGLSNLKPPIVLQEFVNHGGV 181

Query: 187 MFKIYVAGDQSMCVKRKSLPDVEETEEELEKKTEGAMKFSQISRAEEKSEKCNGEAKKED 246
           +FK+YV G+   CVKRKSLPDV  +EEE     EG + FSQ+S         +   KK  
Sbjct: 182 IFKVYVVGEYVKCVKRKSLPDV--SEEEKLGSLEGLLSFSQVSNLATNERTDDKYYKKMH 241

Query: 247 EEEIEMPPEKIVREVSRGLKEAMGIRLFNFDMIRD-RNNGRYYVIDINYLPGFAVLPEYE 306
            +E EMPP+  + +++RGL+ AM + LFNFD+IRD R   RY +IDINY PG+A +P YE
Sbjct: 242 LDETEMPPQSFLTDIARGLRRAMKLNLFNFDVIRDARFGNRYLIIDINYFPGYAKMPGYE 301

Query: 307 PFLTKFFKEVREKKVDVDEVASATENDKQNPRCC 340
             LT FF ++ EKK + +      E D  + R C
Sbjct: 302 TVLTDFFCDIMEKK-EREVEKGLEEKDGADSRGC 332

[Close]

BLAST of Cucsa.318590 vs. TAIR10
Match: AT5G16760.1 (AT5G16760.1 Inositol 1,3,4-trisphosphate 5/6-kinase family protein)

HSP 1 Score: 292.7 bits (748), Expect = 2.9e-79
Identity = 148/313 (47.28%), Postives = 215/313 (68.69%), Query Frame = 1


Query: 10  RLRVGYAFPPNKERNVIRPSLIDYAKLHSVDLVRIDLQTPLLHQGPFHCIIHKLYDDAWA 69
           R  VGYA    K+ + I+PSLI++++   +DLV++D    LL QG   CIIHKLYD  W 
Sbjct: 8   RYLVGYALAAKKQHSFIQPSLIEHSRQRGIDLVKLDPTKSLLEQGKLDCIIHKLYDVYWK 67

Query: 70  ENLQEFASKNPDVVVVDRPDLISKLYNRVSMLDVVSQVK--VSDSDVKIEVPKQILVKQE 129
           ENL EF  K P V V+D P+ I +L+NRVSML+V++Q++  VSDS+ +  VP+Q++V   
Sbjct: 68  ENLHEFREKCPGVPVIDLPEAIERLHNRVSMLEVITQLRFPVSDSE-RFGVPEQVVVMDS 127

Query: 130 DEAIDSIMKLELKFPVIAKPMESDGSAKSHEMSLVFNRRGLKDLNKPVLVQEFVNHGGVM 189
                     ELKFPVIAKP+++DGSAKSH+M L++++ G+K L  P+++QEFVNHGGV+
Sbjct: 128 SVLSGGGALGELKFPVIAKPLDADGSAKSHKMFLIYDQEGMKILKAPIVLQEFVNHGGVI 187

Query: 190 FKIYVAGDQSMCVKRKSLPDVEETEEELEKKTEGAMKFSQISRAEEKSEKCNGEAKKEDE 249
           FK+YV GD   CVKR+SLPD+    EE    ++G++ FSQIS    + +K     +    
Sbjct: 188 FKVYVVGDHVKCVKRRSLPDI---SEEKIGTSKGSLPFSQISNLTAQEDKNIEYGEDRSL 247

Query: 250 EEIEMPPEKIVREVSRGLKEAMGIRLFNFDMIRD-RNNGRYYVIDINYLPGFAVLPEYEP 309
           E++EMPP   + ++++ ++E+MG+ LFNFD+IRD ++  RY +IDINY PG+A +P YEP
Sbjct: 248 EKVEMPPLSFLTDLAKAMRESMGLNLFNFDVIRDAKDANRYLIIDINYFPGYAKMPSYEP 307

Query: 310 FLTKFFKEVREKK 320
            LT+FF ++  KK
Sbjct: 308 VLTEFFWDMVTKK 316

[Close]

BLAST of Cucsa.318590 vs. TAIR10
Match: AT4G33770.1 (AT4G33770.1 Inositol 1,3,4-trisphosphate 5/6-kinase family protein)

HSP 1 Score: 241.5 bits (615), Expect = 7.6e-64
Identity = 122/312 (39.10%), Postives = 196/312 (62.82%), Query Frame = 1


Query: 10  RLRVGYAFPPNKERNVIRPSLIDYAKLHSVDLVRIDLQTPLLHQGPFHCIIHKLYDDAWA 69
           +L VGYA    K+++ ++P L   A+   +  V IDL  PL  QGPF  ++HKL    W 
Sbjct: 80  KLVVGYALTSKKKKSFLQPKLELLARRKGIFFVAIDLNRPLSEQGPFDVVLHKLLGKEWE 139

Query: 70  ENLQEFASKNPDVVVVDRPDLISKLYNRVSMLDVVSQVKVSDSDVKIEVPKQILVKQEDE 129
           E ++++  K+P+V V+D P  I ++YNR SML  ++ +K+SD    + VPKQ++V ++  
Sbjct: 140 EVIEDYQQKHPEVTVLDPPGSIQRIYNRQSMLQGMADLKLSDCSGSLFVPKQMVVLKDSA 199

Query: 130 A-IDSIMKLELKFPVIAKPMESDGSAKSHEMSLVFNRRGLKDLNKPVLVQEFVNHGGVMF 189
           A  D++++  LKFP++AKP+  DG+AKSH++ L ++RR L +L+ P+++QEFVNHGGVMF
Sbjct: 200 ASADAVVEAGLKFPLVAKPLWIDGTAKSHQLYLAYDRRSLAELDPPLVLQEFVNHGGVMF 259

Query: 190 KIYVAGDQSMCVKRKSLPDVEETEEELEKKTEGAMKFSQISRAEEKSEKCNGEAKKEDEE 249
           K++V GD    ++R SLP+V   E   + K +G  +F ++S A   ++  +      D  
Sbjct: 260 KVFVVGDVIKVMRRFSLPNVSNCE---KAKVDGVFQFPRVSSAAASADNAD-----LDPR 319

Query: 250 EIEMPPEKIVREVSRGLKEAMGIRLFNFDMIRDRNNGR-YYVIDINYLPGFAVLPEYEPF 309
             E+PP+  +  + + L+  +G+RLFN DMIR+  +   +YVIDINY PG+  LP+YE  
Sbjct: 320 VAELPPKPFLEALVKELRSLLGLRLFNIDMIREHGSKNVFYVIDINYFPGYGKLPDYEQV 379

Query: 310 LTKFFKEVREKK 320
              FF+ + + K
Sbjct: 380 FVDFFQNLAQVK 383

[Close]

BLAST of Cucsa.318590 vs. TAIR10
Match: AT4G08170.2 (AT4G08170.2 Inositol 1,3,4-trisphosphate 5/6-kinase family protein)

HSP 1 Score: 234.2 bits (596), Expect = 1.2e-61
Identity = 122/312 (39.10%), Postives = 187/312 (59.94%), Query Frame = 1


Query: 6   PSSNRLRVGYAFPPNKERNVIRPSLIDYAKLHSVDLVRIDLQTPLLHQGPFHCIIHKLYD 65
           P    + VGYA    K ++ ++P L   A+   +  V ID   PL  QGPF  ++HK   
Sbjct: 36  PVKKSIIVGYALTSKKIKSFLQPKLEGLARNKGILFVAIDQNKPLSEQGPFDIVLHKQIG 95

Query: 66  DAWAENLQEFASKNPDVVVVDRPDLISKLYNRVSMLDVVSQVKVSDSDVKIEVPKQILVK 125
             W   L+EF   +PDV V+D PD I  L NR SML  V+ + +SDS+ ++ VPKQ+++K
Sbjct: 96  KEWRRILEEFRLAHPDVTVLDPPDAILHLRNRQSMLQCVADMNLSDSNGRVGVPKQLVIK 155

Query: 126 QEDEAI-DSIMKLELKFPVIAKPMESDGSAKSHEMSLVFNRRGLKDLNKPVLVQEFVNHG 185
           ++  +I +++    L+ P++AKP+ +DGSAKSHE+SL +++  L  L  P+++QEFVNHG
Sbjct: 156 KDASSIPEAVNNAGLRLPLVAKPLVADGSAKSHELSLAYDQHSLLKLEPPLVLQEFVNHG 215

Query: 186 GVMFKIYVAGDQSMCVKRKSLPDVEETEEELEKKTEGAMKFSQISRAEEKSEKCNGEAKK 245
           GV+FK+Y+ G+    V+R SLPDV   E     K+ G  +F ++S A   ++  +     
Sbjct: 216 GVLFKVYIVGEAIRVVRRFSLPDVSRRE---LPKSAGVFRFPRVSCAAASADDAD----- 275

Query: 246 EDEEEIEMPPEKIVREVSRGLKEAMGIRLFNFDMIRDR-NNGRYYVIDINYLPGFAVLPE 305
            D    E+PP  ++  +++ L+  +G+RLFN D+IR+     R+YVIDINY PG+  +PE
Sbjct: 276 LDPSIAELPPRPLLERLAKELRRGLGLRLFNLDIIREHGTRDRFYVIDINYFPGYGKMPE 335

Query: 306 YEPFLTKFFKEV 316
           YE   T F   V
Sbjct: 336 YEHVFTDFLLSV 339

[Close]

BLAST of Cucsa.318590 vs. TAIR10
Match: AT2G43980.1 (AT2G43980.1 inositol 1,3,4-trisphosphate 5/6-kinase 4)

HSP 1 Score: 91.3 bits (225), Expect = 1.3e-18
Identity = 54/201 (26.87%), Postives = 98/201 (48.76%), Query Frame = 1


Query: 127 EDEAIDSIMKLELKFPVIAKPMESDGSAKSHEMSLVFNRRGLKDLNKPV--LVQEFVNHG 186
           E +   ++ +  L  P I KP  + G A +H M++VF     K+LN PV  ++QE+V+H 
Sbjct: 296 EPDLAQNLSRAGLSLPCIVKPQVACGVADAHSMAIVFRVEDFKNLNTPVPAIIQEYVDHS 355

Query: 187 GVMFKIYVAGDQSMCVKRKSLPDVEETEEELEKKTEGAMKFSQISRAEEKSEKCNGEAKK 246
             +FK YV G+      +KS+P      +  E+     + F  +      S      A +
Sbjct: 356 SRIFKFYVLGETIFHAVKKSIPSSSSLRKSAEENGLKPILFDSLKSLPVDS------ANQ 415

Query: 247 EDEEEIEMPPEKIVREVSRGLKEAMGIRLFNFDMIRDRNNGRYYVIDINYLPGFAVLPE- 306
               EI++   ++V E +  L++ + + +F FD++     G + ++D+NYLP F  +P+ 
Sbjct: 416 NPVSEIDL---ELVTEAATWLRKKLDLTIFGFDVVIQEGTGDHVIVDLNYLPSFKEVPDN 475

Query: 307 ------YEPFLTKFFKEVREK 319
                 +E    +F + V+EK
Sbjct: 476 IAVPAFWEAIRNRFDQHVQEK 487

[Close]

BLAST of Cucsa.318590 vs. NCBI nr
Match: gi|449460744|ref|XP_004148105.1| (PREDICTED: inositol-tetrakisphosphate 1-kinase 1-like [Cucumis sativus])

HSP 1 Score: 686.8 bits (1771), Expect = 1.9e-194
Identity = 343/343 (100.00%), Postives = 343/343 (100.00%), Query Frame = 1


Query: 1   MASNSPSSNRLRVGYAFPPNKERNVIRPSLIDYAKLHSVDLVRIDLQTPLLHQGPFHCII 60
           MASNSPSSNRLRVGYAFPPNKERNVIRPSLIDYAKLHSVDLVRIDLQTPLLHQGPFHCII
Sbjct: 1   MASNSPSSNRLRVGYAFPPNKERNVIRPSLIDYAKLHSVDLVRIDLQTPLLHQGPFHCII 60

Query: 61  HKLYDDAWAENLQEFASKNPDVVVVDRPDLISKLYNRVSMLDVVSQVKVSDSDVKIEVPK 120
           HKLYDDAWAENLQEFASKNPDVVVVDRPDLISKLYNRVSMLDVVSQVKVSDSDVKIEVPK
Sbjct: 61  HKLYDDAWAENLQEFASKNPDVVVVDRPDLISKLYNRVSMLDVVSQVKVSDSDVKIEVPK 120

Query: 121 QILVKQEDEAIDSIMKLELKFPVIAKPMESDGSAKSHEMSLVFNRRGLKDLNKPVLVQEF 180
           QILVKQEDEAIDSIMKLELKFPVIAKPMESDGSAKSHEMSLVFNRRGLKDLNKPVLVQEF
Sbjct: 121 QILVKQEDEAIDSIMKLELKFPVIAKPMESDGSAKSHEMSLVFNRRGLKDLNKPVLVQEF 180

Query: 181 VNHGGVMFKIYVAGDQSMCVKRKSLPDVEETEEELEKKTEGAMKFSQISRAEEKSEKCNG 240
           VNHGGVMFKIYVAGDQSMCVKRKSLPDVEETEEELEKKTEGAMKFSQISRAEEKSEKCNG
Sbjct: 181 VNHGGVMFKIYVAGDQSMCVKRKSLPDVEETEEELEKKTEGAMKFSQISRAEEKSEKCNG 240

Query: 241 EAKKEDEEEIEMPPEKIVREVSRGLKEAMGIRLFNFDMIRDRNNGRYYVIDINYLPGFAV 300
           EAKKEDEEEIEMPPEKIVREVSRGLKEAMGIRLFNFDMIRDRNNGRYYVIDINYLPGFAV
Sbjct: 241 EAKKEDEEEIEMPPEKIVREVSRGLKEAMGIRLFNFDMIRDRNNGRYYVIDINYLPGFAV 300

Query: 301 LPEYEPFLTKFFKEVREKKVDVDEVASATENDKQNPRCCSCFH 344
           LPEYEPFLTKFFKEVREKKVDVDEVASATENDKQNPRCCSCFH
Sbjct: 301 LPEYEPFLTKFFKEVREKKVDVDEVASATENDKQNPRCCSCFH 343

[Close]

BLAST of Cucsa.318590 vs. NCBI nr
Match: gi|659118740|ref|XP_008459279.1| (PREDICTED: inositol-tetrakisphosphate 1-kinase 1-like [Cucumis melo])

HSP 1 Score: 621.7 bits (1602), Expect = 7.7e-175
Identity = 317/342 (92.69%), Postives = 326/342 (95.32%), Query Frame = 1


Query: 1   MASNSPSSNRLRVGYAFPPNKERNVIRPSLIDYAKLHSVDLVRIDLQTPLLHQGPFHCII 60
           MASNSPSSNRLRVGYAFPPNKERNVIRPSLIDYAK H VDLVRIDLQTPLLHQGPFHCII
Sbjct: 1   MASNSPSSNRLRVGYAFPPNKERNVIRPSLIDYAKFHGVDLVRIDLQTPLLHQGPFHCII 60

Query: 61  HKLYDDAWAENLQEFASKNPDVVVVDRPDLISKLYNRVSMLDVVSQVKVSDSDVKIEVPK 120
           HKLYDDAWAENLQ+FASKNPDVVVVDRPDLISKLYNRVSMLDVVSQVKVSDS V+IEVPK
Sbjct: 61  HKLYDDAWAENLQDFASKNPDVVVVDRPDLISKLYNRVSMLDVVSQVKVSDSGVRIEVPK 120

Query: 121 QILVKQEDEAIDSIMKLELKFPVIAKPMESDGSAKSHEMSLVFNRRGLKDLNKPVLVQEF 180
           QILVK+E+EAIDSIM LELKFPVIAKPMESDGSAKSHEMSLVFNRRGLK+LN PVL+QEF
Sbjct: 121 QILVKKEEEAIDSIMNLELKFPVIAKPMESDGSAKSHEMSLVFNRRGLKELNTPVLLQEF 180

Query: 181 VNHGGVMFKIYVAGDQSMCVKRKSLPDVEETEEELEKKTEGAMKFSQISRAEEKSEKCNG 240
           VNHGGVMFKIYVAGDQS+CVKRKSLPDVE TEEELEKKTEGAMKFSQISRAEEKSEKCNG
Sbjct: 181 VNHGGVMFKIYVAGDQSVCVKRKSLPDVEGTEEELEKKTEGAMKFSQISRAEEKSEKCNG 240

Query: 241 EAKKEDEEEIEMPPEKIVREVSRGLKEAMGIRLFNFDMIRDRNNGRYYVIDINYLPGFAV 300
           EAKKE E+EIEMPPEKIVREVSRGLKEAMGIRLFNFDMIRD  NG YYVIDINYLPGFAV
Sbjct: 241 EAKKE-EKEIEMPPEKIVREVSRGLKEAMGIRLFNFDMIRD-GNGTYYVIDINYLPGFAV 300

Query: 301 LPEYEPFLTKFFKEVREKKVDVDEVASATENDKQNPRCCSCF 343
           LPEYEPFLTKFFKEV EKK  VDEVASA E+DK  PRCCSCF
Sbjct: 301 LPEYEPFLTKFFKEVTEKK--VDEVASAIEDDKAKPRCCSCF 338

[Close]

BLAST of Cucsa.318590 vs. NCBI nr
Match: gi|449460700|ref|XP_004148083.1| (PREDICTED: inositol-tetrakisphosphate 1-kinase 1-like [Cucumis sativus])

HSP 1 Score: 341.3 bits (874), Expect = 2.0e-90
Identity = 180/337 (53.41%), Postives = 230/337 (68.25%), Query Frame = 1


Query: 7   SSNRLRVGYAFPPNKERNVIRPSLIDYAKLHSVDLVRIDLQTPLLHQGPFHCIIHKLYDD 66
           SS R R+GYA    KER+ I PSL+DYAKLH +D VRID   PL  Q PFHCIIHKLYD 
Sbjct: 2   SSPRFRIGYALSLKKERSFILPSLVDYAKLHGIDFVRIDPLLPLTDQTPFHCIIHKLYDP 61

Query: 67  AWAENLQEFASKNPDVVVVDRPDLISKLYNRVSMLDVVSQVKVSDSDVKIEVPKQILVKQ 126
           +W + LQ+F S+ PDVVVVD P+LIS+L NR SML+VV +VKV   D +IE PKQ++V  
Sbjct: 62  SWVQQLQDFTSQFPDVVVVDPPELISRLLNRDSMLEVVKEVKVPQGDERIETPKQVVVND 121

Query: 127 ED----EAIDSIMKLELKFPVIAKPMESDGSAKSHEMSLVFNRRGLKDLNKPVLVQEFVN 186
            D      +++   L LKFP+IAKP+ES+GSAKSH++ LV N  GLK LN P+++QEFVN
Sbjct: 122 LDVVLKNGLNTFSDLGLKFPIIAKPLESNGSAKSHQLCLVSNDSGLKGLNAPIVLQEFVN 181

Query: 187 HGGVMFKIYVAGDQSMCVKRKSLPDVEETEEELEKKTEGAMKFSQISRAEEKSEKCNGEA 246
           HGGV+FK+YV G+  +CV RKSLPD+     E  KK +    FSQIS         N  A
Sbjct: 182 HGGVVFKVYVVGECVVCVTRKSLPDI---GPEDVKKLDAVSSFSQIS---------NSGA 241

Query: 247 KKEDEEEIEMPPEKIVREVSRGLKEAMGIRLFNFDMIRDRN-NGRYYVIDINYLPGFAVL 306
           + +DE  +EMP  + V  V+ GL+EA G+RLFNFD+IRD N + RY VIDINYLPG+A +
Sbjct: 242 QGDDEGNVEMPSLEFVMHVAAGLREATGLRLFNFDLIRDSNDHNRYLVIDINYLPGYAKM 301

Query: 307 PEYEPFLTKFFKEVREKKVDVDEVASATENDKQNPRC 339
           P YEPFLTKFF +V + +      +S+  N+ +   C
Sbjct: 302 PNYEPFLTKFFLDVVQNRTVGGAFSSSYVNENEVVFC 326

[Close]

BLAST of Cucsa.318590 vs. NCBI nr
Match: gi|659118544|ref|XP_008459175.1| (PREDICTED: inositol-tetrakisphosphate 1-kinase 1-like [Cucumis melo])

HSP 1 Score: 339.0 bits (868), Expect = 9.9e-90
Identity = 179/337 (53.12%), Postives = 230/337 (68.25%), Query Frame = 1


Query: 7   SSNRLRVGYAFPPNKERNVIRPSLIDYAKLHSVDLVRIDLQTPLLHQGPFHCIIHKLYDD 66
           SS R+R+GYA    KER+ I PSL+DYAKLH +D VRID   PL  Q PFHCIIHKLYD 
Sbjct: 2   SSPRIRIGYALSLKKERSFILPSLVDYAKLHGIDFVRIDPLLPLTDQTPFHCIIHKLYDP 61

Query: 67  AWAENLQEFASKNPDVVVVDRPDLISKLYNRVSMLDVVSQVKVSDSDVKIEVPKQILVKQ 126
           +W + LQ+F S+ PDVVVVD P+LIS+L NR SML+VV +VKV   D +IE PKQ++V  
Sbjct: 62  SWVQQLQDFTSQFPDVVVVDPPELISRLLNRDSMLEVVKEVKVPQGDERIETPKQVVVND 121

Query: 127 ED----EAIDSIMKLELKFPVIAKPMESDGSAKSHEMSLVFNRRGLKDLNKPVLVQEFVN 186
            D      ++    L LKFPVIAKP+ES+GSA+SH++ LV N  GLK LN P+++QEFVN
Sbjct: 122 LDVLMKNGLNMFSDLGLKFPVIAKPLESNGSARSHQLCLVSNDSGLKGLNAPIVLQEFVN 181

Query: 187 HGGVMFKIYVAGDQSMCVKRKSLPDVEETEEELEKKTEGAMKFSQISRAEEKSEKCNGEA 246
           HGGV+FK+YV G+  +CV RKSLPD+     E  KK +    FSQIS         N  A
Sbjct: 182 HGGVVFKVYVVGECVVCVTRKSLPDI---APEDVKKLDAVSSFSQIS---------NSGA 241

Query: 247 KKEDEEEIEMPPEKIVREVSRGLKEAMGIRLFNFDMIRD-RNNGRYYVIDINYLPGFAVL 306
           + +DE  +EMP  + V  V+ GL+EA G+RLFNFD+IRD  ++ RY VIDINYLPG+A +
Sbjct: 242 QGDDEGNVEMPSLEFVMHVAAGLREATGLRLFNFDLIRDSSDHNRYLVIDINYLPGYAKM 301

Query: 307 PEYEPFLTKFFKEVREKKVDVDEVASATENDKQNPRC 339
           P YEPFLTKFF +V + +      +S+  N+ +   C
Sbjct: 302 PNYEPFLTKFFLDVVQNRTVGGAFSSSYVNENEVVFC 326

[Close]

BLAST of Cucsa.318590 vs. NCBI nr
Match: gi|225454733|ref|XP_002270915.1| (PREDICTED: inositol-tetrakisphosphate 1-kinase 1 [Vitis vinifera])

HSP 1 Score: 317.4 bits (812), Expect = 3.1e-83
Identity = 163/315 (51.75%), Postives = 214/315 (67.94%), Query Frame = 1


Query: 10  RLRVGYAFPPNKERNVIRPSLIDYAKLHSVDLVRIDLQTPLLHQGPFHCIIHKLYDDAWA 69
           R  VGYA  P K  + I+PSL+D+AK   +DLVRIDL  PL+ QGPF CIIHK+ D+ W 
Sbjct: 7   RFTVGYALAPKKVSSFIQPSLVDHAKQRGIDLVRIDLDKPLIEQGPFDCIIHKMNDEDWK 66

Query: 70  ENLQEFASKNPDVVVVDRPDLISKLYNRVSMLDVVSQVKVSDSDVKIEVPKQILVKQEDE 129
             L+EF++KNP+VV++D PD I +L++R+SML+VV ++K+ +      +PKQI++   + 
Sbjct: 67  NQLEEFSTKNPNVVIIDPPDAIEQLHSRISMLEVVKELKIPEGTESFGIPKQIVIYDPES 126

Query: 130 AIDSIMKLELKFPVIAKPMESDGSAKSHEMSLVFNRRGLKDLNKPVLVQEFVNHGGVMFK 189
            +DS +   L FPVIAKP+ +DGSAKSH+MSL FN  GLK L  P+++QEFVNHGGV+FK
Sbjct: 127 LLDSKVLDGLSFPVIAKPLVADGSAKSHKMSLAFNGEGLKKLTTPIVLQEFVNHGGVIFK 186

Query: 190 IYVAGDQSMCVKRKSLPDVEETEEELEKKTEGAMKFSQISRAEEKSEKCNGEAKKED--- 249
           +YV GD   CVKR+SLPDV     E    +EG + FSQIS      E   GE   ED   
Sbjct: 187 VYVVGDHVTCVKRRSLPDV---SVEKLGTSEGLLTFSQISNLTATQEP--GENDCEDIMN 246

Query: 250 -EEEIEMPPEKIVREVSRGLKEAMGIRLFNFDMIRDRNNG-RYYVIDINYLPGFAVLPEY 309
             EE EMPP   V E++ GL++AMG+ LFNFD+IRD   G RY VIDINY PG+A +P Y
Sbjct: 247 HVEEAEMPPLNFVNEIAIGLRKAMGLNLFNFDVIRDAKLGNRYLVIDINYFPGYAKMPSY 306

Query: 310 EPFLTKFFKEVREKK 320
           E  LT FF ++  +K
Sbjct: 307 ETVLTDFFWDIVHRK 316

The following BLAST results are available for this feature:

BLAST of Cucsa.318590 vs. Swiss-Prot


Match Name	E-value	Identity	Description
ITPK1_ARATH	5.1e-78	47.28	Inositol-tetrakisphosphate 1-kinase 1 OS=Arabidopsis thaliana GN=ITPK1 PE=1 SV=1	[more]
ITPK1_MAIZE	5.8e-74	46.01	Inositol-tetrakisphosphate 1-kinase 1 OS=Zea mays GN=ITPK1 PE=2 SV=1	[more]
ITPK5_ORYSJ	1.3e-70	44.97	Inositol-tetrakisphosphate 1-kinase 5 OS=Oryza sativa subsp. japonica GN=ITPK5 P...	[more]
ITPK5_ORYSI	1.3e-70	44.97	Inositol-tetrakisphosphate 1-kinase 5 OS=Oryza sativa subsp. indica GN=ITPK5 PE=...	[more]
ITPK4_ORYSJ	5.9e-66	44.83	Inositol-tetrakisphosphate 1-kinase 4 OS=Oryza sativa subsp. japonica GN=ITPK4 P...	[more]

BLAST of Cucsa.318590 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LRI5_CUCSA	1.4e-194	100.00	Inositol-tetrakisphosphate 1-kinase OS=Cucumis sativus GN=Csa_2G439740 PE=3 SV=1	[more]
A0A0A0LTT9_CUCSA	1.4e-90	53.41	Inositol-tetrakisphosphate 1-kinase OS=Cucumis sativus GN=Csa_2G439240 PE=3 SV=1	[more]
F6HEE3_VITVI	2.2e-83	51.75	Inositol-tetrakisphosphate 1-kinase OS=Vitis vinifera GN=VIT_16s0039g01700 PE=3 ...	[more]
A5BKJ6_VITVI	1.8e-82	51.75	Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_018923 PE=4 SV=1	[more]
W9R373_9ROSA	2.4e-82	46.71	Inositol-tetrakisphosphate 1-kinase OS=Morus notabilis GN=L484_017781 PE=3 SV=1	[more]

BLAST of Cucsa.318590 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G16760.1	2.9e-79	47.28	Inositol 1,3,4-trisphosphate 5/6-kinase family protein	[more]
AT4G33770.1	7.6e-64	39.10	Inositol 1,3,4-trisphosphate 5/6-kinase family protein	[more]
AT4G08170.2	1.2e-61	39.10	Inositol 1,3,4-trisphosphate 5/6-kinase family protein	[more]
AT2G43980.1	1.3e-18	26.87	inositol 1,3,4-trisphosphate 5/6-kinase 4	[more]

BLAST of Cucsa.318590 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449460744\|ref\|XP_004148105.1\|	1.9e-194	100.00	PREDICTED: inositol-tetrakisphosphate 1-kinase 1-like [Cucumis sativus]	[more]
gi\|659118740\|ref\|XP_008459279.1\|	7.7e-175	92.69	PREDICTED: inositol-tetrakisphosphate 1-kinase 1-like [Cucumis melo]	[more]
gi\|449460700\|ref\|XP_004148083.1\|	2.0e-90	53.41	PREDICTED: inositol-tetrakisphosphate 1-kinase 1-like [Cucumis sativus]	[more]
gi\|659118544\|ref\|XP_008459175.1\|	9.9e-90	53.12	PREDICTED: inositol-tetrakisphosphate 1-kinase 1-like [Cucumis melo]	[more]
gi\|225454733\|ref\|XP_002270915.1\|	3.1e-83	51.75	PREDICTED: inositol-tetrakisphosphate 1-kinase 1 [Vitis vinifera]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR008656	Inositol_tetrakis-P_1-kinase
IPR011761	ATP-grasp
IPR013816	ATP-grasp fold, subdomain 2

Vocabulary: **Molecular Function**
Term	Definition
GO:0052725	inositol-1,3,4-trisphosphate 6-kinase activity
GO:0052726	inositol-1,3,4-trisphosphate 5-kinase activity
GO:0000287	magnesium ion binding
GO:0005524	ATP binding
GO:0047325	inositol tetrakisphosphate 1-kinase activity
GO:0046872	metal ion binding

Vocabulary: **Cellular Component**
Term	Definition
GO:0005622	intracellular

Vocabulary: **Biological Process**
Term	Definition
GO:0032957	inositol trisphosphate metabolic process

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032957	inositol trisphosphate metabolic process
biological_process	GO:0016310	phosphorylation
cellular_component	GO:0005622	intracellular
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0052726	inositol-1,3,4-trisphosphate 5-kinase activity
molecular_function	GO:0052725	inositol-1,3,4-trisphosphate 6-kinase activity
molecular_function	GO:0047325	inositol tetrakisphosphate 1-kinase activity
molecular_function	GO:0000287	magnesium ion binding
molecular_function	GO:0046872	metal ion binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsa.318590.1	Cucsa.318590.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008656	Inositol-tetrakisphosphate 1-kinase	PIR	PIRSF038186	ITPK	coord: 1..328 score: 9.5E
IPR008656	Inositol-tetrakisphosphate 1-kinase	PFAM	PF05770	Ins134_P3_kin	coord: 8..313 score: 9.1E
IPR011761	ATP-grasp fold	PROFILE	PS50975	ATP_GRASP	coord: 108..321 score: 18
IPR013816	ATP-grasp fold, subdomain 2	GENE3D	G3DSA:3.30.470.20		coord: 113..297 score: 8.
None	No IPR available	unknown	Coil	Coil	coord: 228..248 scor
None	No IPR available	PANTHER	PTHR14217	FAMILY NOT NAMED	coord: 1..332 score: 1.0E
None	No IPR available	PANTHER	PTHR14217:SF5	INOSITOL-TETRAKISPHOSPHATE 1-KINASE 1	coord: 1..332 score: 1.0E
None	No IPR available	unknown	SSF56059	Glutathione synthetase ATP-binding domain-like	coord: 88..207 score: 2.42E-14coord: 235..302 score: 2.42

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cucsa.318590	Cla013010	Watermelon (97103) v1	cgywmB580
Cucsa.318590	Csa2G439240	Cucumber (Chinese Long) v2	cgycuB456
Cucsa.318590	MELO3C021870	Melon (DHL92) v3.5.1	cgymeB524
Cucsa.318590	ClCG09G017090	Watermelon (Charleston Gray)	cgywcgB551
Cucsa.318590	CSPI02G27880	Wild cucumber (PI 183967)	cgycpiB481
Cucsa.318590	CmaCh20G009050	Cucurbita maxima (Rimu)	cgycmaB0881
Cucsa.318590	CmoCh20G009130	Cucurbita moschata (Rifu)	cgycmoB0878
Cucsa.318590	Lsi10G002750	Bottle gourd (USVL1VR-Ls)	cgylsiB520
Cucsa.318590	Cp4.1LG16g02020	Cucurbita pepo (Zucchini)	cgycpeB0837
Cucsa.318590	MELO3C021870.2	Melon (DHL92) v3.6.1	cgymedB526
Cucsa.318590	CsaV3_2G036570	Cucumber (Chinese Long) v3	cgycucB495
Cucsa.318590	Cla97C09G178660	Watermelon (97103) v2	cgywmbB556
Cucsa.318590	Carg23602	Silver-seed gourd	carcgyB0908

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cucsa.318590	Cucurbita maxima (Rimu)	cgycmaB0882
Cucsa.318590	Cucurbita moschata (Rifu)	cgycmoB0879
Cucsa.318590	Melon (DHL92) v3.5.1	cgymeB525
Cucsa.318590	Cucurbita pepo (Zucchini)	cgycpeB0838
Cucsa.318590	Melon (DHL92) v3.6.1	cgymedB525
Cucsa.318590	Silver-seed gourd	carcgyB1170