BLAST of Cucsa.318540 vs. Swiss-Prot
Match:
Y5673_ARATH (WEB family protein At5g16730, chloroplastic OS=Arabidopsis thaliana GN=At5g16730 PE=1 SV=1)
HSP 1 Score: 662.5 bits (1708), Expect = 5.2e-189
Identity = 379/728 (52.06%), Postives = 511/728 (70.19%), Query Frame = 1
Query: 3 QAGLEEAHKKEEEWEKEIEAVRSQHALDVAALLSTSQELQRVKMELAMTTDAKNQALSHA 62
+AG+E EEE +KE+E V++QHA D AAL++ QEL+++ ELA DAK++ALS A
Sbjct: 165 EAGIEAVQNNEEELKKELETVKNQHASDSAALVAVRQELEKINEELAAAFDAKSKALSQA 224
Query: 63 DDATKIAEIHVEKVEILSGELTRLKALLDSKLEMQSNENGQLIMKLKSEIDSLNLELEKA 122
+DA+K AEIH EKV+ILS ELTRLKALLDS E + + +++ KL+ EI L +LE A
Sbjct: 225 EDASKTAEIHAEKVDILSSELTRLKALLDSTREKTAISDNEMVAKLEDEIVVLKRDLESA 284
Query: 123 KSYAEMVKEKEVSIERLNSELKAAKMAETCYEETIMDKDASIEQLNIDLEAAKMAETYAH 182
+ + VKEKE+ +E+L N+DLEAAKMAE+ AH
Sbjct: 285 RGFEAEVKEKEMIVEKL----------------------------NVDLEAAKMAESNAH 344
Query: 183 GLVEEWKNRAEEMETKLDSANKLERSASESLDSVMKQLEHNNDLLHNAELEIAALKEKVG 242
L EW+++A+E+E +L+ ANKLERSAS SL+SVMKQLE +ND LH+ E EI LKE++
Sbjct: 345 SLSNEWQSKAKELEEQLEEANKLERSASVSLESVMKQLEGSNDKLHDTETEITDLKERIV 404
Query: 243 LLEMTVKRQKEDLKESEHHLHRAKEEASEMEKLVASLRAQLETVNEEKTQALNNEKLAAS 302
LE TV +QKEDL+ SE L +EE S+ EK V L+++LETV EEK +AL E+ A S
Sbjct: 405 TLETTVAKQKEDLEVSEQRLGSVEEEVSKNEKEVEKLKSELETVKEEKNRALKKEQDATS 464
Query: 303 SVQSLLEEKNQLLNELETSKDEEEKSKKAMESLASALHEISTEARETKEKLLSSQADQEN 362
VQ L EEK++LL++LE+SK+EEEKSKKAMESLASALHE+S+E RE KEKLL SQ D E
Sbjct: 465 RVQRLSEEKSKLLSDLESSKEEEEKSKKAMESLASALHEVSSEGRELKEKLL-SQGDHE- 524
Query: 363 YESQIENLKLVLKATNEKYENMLENSNHEIDILTSTIEKSKHEYENSKAEWEEKELHLVD 422
YE+QI++LKLV+KATNEKYENML+ + HEID+L S +E++K +E+SK +WE KE +LV+
Sbjct: 525 YETQIDDLKLVIKATNEKYENMLDEARHEIDVLVSAVEQTKKHFESSKKDWEMKEANLVN 584
Query: 423 AVKKSEEENSSLDKEIDRLVNLLKQTEEEACKMREEEAQLKDSLKEVEAEVIYLQEALGE 482
VKK EE+ +S+ KE++RL NLLK+TEEEA ++EAQ KDSLKEVE E++YLQE LGE
Sbjct: 585 YVKKMEEDVASMGKEMNRLDNLLKRTEEEADAAWKKEAQTKDSLKEVEEEIVYLQETLGE 644
Query: 483 AKSESMKLKESLLDKENEFQSIHQENEELLTREAASLKKVDELSKLLEEA-SAKKQ-TME 542
AK+ESMKLKE+LLDKE EFQ++ ENE+L +E SLKK++ELSKLLEEA AKKQ E
Sbjct: 645 AKAESMKLKENLLDKETEFQNVIHENEDLKAKEDVSLKKIEELSKLLEEAILAKKQPEEE 704
Query: 543 NGEPTDSEKDYDLLPKVVEFSEENGKR--QEEKTKVEPPIPIEHEEHKFEFPWVGNGASD 602
NGE ++SEKDYDLLPKVVEFS ENG R +E+ KVE ++HE + + + +
Sbjct: 705 NGELSESEKDYDLLPKVVEFSSENGHRSVEEKSAKVE---TLDHEPPQEQISNGNSNGNG 764
Query: 603 EKTEKTDSAATLQNGNDKPKEAEKKEKEDDSVKVEYKMWESCKIEKKEFSQEGGEPEHES 662
+ ++ + ++ + K+ + + +DDSV+V +KMWESC+IEKKE + + E ES
Sbjct: 765 MEEKEVNGKPEVETEKKEKKDESQDDDKDDSVEVIFKMWESCQIEKKEAFPD-KKSELES 824
Query: 663 IDDETDSKPEGGESFDPINGVSSENLDDGGHSPSKQQE--QQQQQKKKKPLLKKFGYLLK 722
++E DS D + S+EN+D+ G++ + + + +++ KKKK LL K G LLK
Sbjct: 825 QEEEEDS-----SKIDESDKTSTENIDETGNALTAEDQLTMEKKIKKKKTLLGKVGNLLK 853
Query: 723 KKNSVNQK 725
KK VNQK
Sbjct: 885 KKAPVNQK 853
BLAST of Cucsa.318540 vs. Swiss-Prot
Match:
Y3293_ARATH (WEB family protein At3g02930, chloroplastic OS=Arabidopsis thaliana GN=At3g02930 PE=2 SV=1)
HSP 1 Score: 644.0 bits (1660), Expect = 1.9e-183
Identity = 376/725 (51.86%), Postives = 496/725 (68.41%), Query Frame = 1
Query: 3 QAGLEEAHKKEEEWEKEIEAVRSQHALDVAALLSTSQELQRVKMELAMTTDAKNQALSHA 62
+AG+E +KEEE +KE+E V++QHA + A LL +QEL+ V ELA DAK++AL A
Sbjct: 154 EAGIEAVQRKEEELKKELENVKNQHASESATLLLVTQELENVNQELANAKDAKSKALCRA 213
Query: 63 DDATKIAEIHVEKVEILSGELTRLKALLDSKLEMQSNENGQLIMKLKSEIDSLNLELEKA 122
DDA+K+A IH EKVEILS EL RLKALLDS E + ++ +KL +EI L +LE A
Sbjct: 214 DDASKMAAIHAEKVEILSSELIRLKALLDSTREKEIISKNEIALKLGAEIVDLKRDLENA 273
Query: 123 KSYAEMVKEKEVSIERLNSELKAAKMAETCYEETIMDKDASIEQLNIDLEAAKMAETYAH 182
+S VKE E+ IE QLN+DLEAAKMAE+YAH
Sbjct: 274 RSLEAKVKELEMIIE----------------------------QLNVDLEAAKMAESYAH 333
Query: 183 GLVEEWKNRAEEMETKLDSANKLERSASESLDSVMKQLEHNNDLLHNAELEIAALKEKVG 242
G +EW+N+A+E+E +L+ ANKLE+ AS SL SV KQLE +N LH+ E EI LKEK+
Sbjct: 334 GFADEWQNKAKELEKRLEEANKLEKCASVSLVSVTKQLEVSNSRLHDMESEITDLKEKIE 393
Query: 243 LLEMTVKRQKEDLKESEHHLHRAKEEASEMEKLVASLRAQLETVNEEKTQALNNEKLAAS 302
LLEMTV QK DL++SE L A+EE+S+ EK L+ +LETVNEEKTQAL E+ A S
Sbjct: 394 LLEMTVASQKVDLEKSEQKLGIAEEESSKSEKEAEKLKNELETVNEEKTQALKKEQDATS 453
Query: 303 SVQSLLEEKNQLLNELETSKDEEEKSKKAMESLASALHEISTEARETKEKLLSSQADQEN 362
SVQ LLEEK ++L+ELE+SK+EEEKSKKAMESLASALHE+S+E+RE KEKLLS + DQ N
Sbjct: 454 SVQRLLEEKKKILSELESSKEEEEKSKKAMESLASALHEVSSESRELKEKLLS-RGDQ-N 513
Query: 363 YESQIENLKLVLKATNEKYENMLENSNHEIDILTSTIEKSKHEYENSKAEWEEKELHLVD 422
YE+QIE+LKLV+KATN KYENML+ + HEID+L + +E++K ++E++ +WE +E LV+
Sbjct: 514 YETQIEDLKLVIKATNNKYENMLDEARHEIDVLVNAVEQTKKQFESAMVDWEMREAGLVN 573
Query: 423 AVKKSEEENSSLDKEIDRLVNLLKQTEEEACKMREEEAQLKDSLKEVEAEVIYLQEALGE 482
VK+ +EE SS+ KE++RL NL+K+T+EEA E+E+Q++D LKEVE EVIYLQE L E
Sbjct: 574 HVKEFDEEVSSMGKEMNRLGNLVKRTKEEADASWEKESQMRDCLKEVEDEVIYLQETLRE 633
Query: 483 AKSESMKLKESLLDKENEFQSIHQENEELLTREAASLKKVDELSKLLEEASAKKQTMENG 542
AK+E++KLK +LDKE EFQSI EN+EL ++ SLKK+ ELS+LLEEA AKK ENG
Sbjct: 634 AKAETLKLKGKMLDKETEFQSIVHENDELRVKQDDSLKKIKELSELLEEALAKKHIEENG 693
Query: 543 EPTDSEKDYDLLPKVVEFSEENGKR--QEEKTKVEPPIPIEHEEHKFEFPWVGNGASDEK 602
E ++SEKDYDLLPKVVEFSEENG R +E+ +KVE ++ K E E
Sbjct: 694 ELSESEKDYDLLPKVVEFSEENGYRSAEEKSSKVE---TLDGMNMKLE----------ED 753
Query: 603 TEKTDSAATLQNGNDKPKEAEKKEKEDDSVKVEYKMWESCKIEKKE-FSQEGGEPEHESI 662
TEK KE +++ ED++V+VE+KMWESC+IEKKE F +E + E E +
Sbjct: 754 TEK--------------KEKKERSPEDETVEVEFKMWESCQIEKKEVFHKESAKEEEEDL 806
Query: 663 DDETDSKPEGGESFDPINGVSSENLDDGGHSPSKQQEQQQQQKKKKPLLKKFGYLLKKKN 722
+ S + P+NG++ E+ + +++++KKKK L K G LLKKK
Sbjct: 814 NVVDQS-----QKTSPVNGLTGED----------ELLKEKEKKKKKTLFGKVGNLLKKKG 806
Query: 723 SVNQK 725
VNQK
Sbjct: 874 PVNQK 806
HSP 2 Score: 36.6 bits (83), Expect = 1.4e+00
Identity = 35/137 (25.55%), Postives = 65/137 (47.45%), Query Frame = 1
Query: 411 AEWEEKELHLVDAVKKSEEENSSLDKE----IDRLVNLLKQTEEEACKMREEEAQLKDSL 470
+E + + + + + +KK+ E +SL+ E +D+L K+ EE + K+ E K SL
Sbjct: 83 SESQPQSVQIKEDLKKANELIASLENEKAKALDQLKEARKEAEEASEKLDEALEAQKKSL 142
Query: 471 KEVEAEVIYLQEALGEA---KSESMKLK------------ESLLDKENEFQSIHQENEEL 529
+ E E + EA EA K E +K + +LL E ++++QE
Sbjct: 143 ENFEIEKFEVVEAGIEAVQRKEEELKKELENVKNQHASESATLLLVTQELENVNQELANA 202
BLAST of Cucsa.318540 vs. Swiss-Prot
Match:
Y1501_ARATH (Putative WEB family protein At1g65010, chloroplastic OS=Arabidopsis thaliana GN=At1g65010 PE=1 SV=1)
HSP 1 Score: 525.8 bits (1353), Expect = 7.6e-148
Identity = 317/726 (43.66%), Postives = 453/726 (62.40%), Query Frame = 1
Query: 1 MEQAGLEEAHKKEEEWEKEIEAVRSQHALDVAALLSTSQELQRVKMELAMTTDAKNQALS 60
+EQAGLE KK+ + E+E++RSQHALD++ALLST++ELQRVK EL+MT DAKN+ALS
Sbjct: 153 LEQAGLEAVQKKDVTSKNELESIRSQHALDISALLSTTEELQRVKHELSMTADAKNKALS 212
Query: 61 HADDATKIAEIHVEKVEILSGELTRLKALLDSKLEMQSNENGQLIMKLKSEIDSLNLELE 120
HA++ATKIAEIH EK EIL+ EL RLKALL SK E ++ E +++ KLKSEI+ L ELE
Sbjct: 213 HAEEATKIAEIHAEKAEILASELGRLKALLGSKEEKEAIEGNEIVSKLKSEIELLRGELE 272
Query: 121 KAKSYAEMVKEKEVSIERLNSELKAAKMAETCYEETIMDKDASIEQLNIDLEAAKMAETY 180
K +KE+E +EQL +DLEAAKMAE+
Sbjct: 273 KVSILESSLKEQE----------------------------GLVEQLKVDLEAAKMAESC 332
Query: 181 AHGLVEEWKNRAEEMETKLDSANKLERSASESLDSVMKQLEHNNDLLHNAELEIAALKEK 240
+ VEEWKN+ E+E +++ +N+ + SASES++SVMKQL N +LH + + AA KEK
Sbjct: 333 TNSSVEEWKNKVHELEKEVEESNRSKSSASESMESVMKQLAELNHVLHETKSDNAAQKEK 392
Query: 241 VGLLEMTVKRQKEDLKESEHHLHRAKEEASEMEKLVASLRAQLETVNEEKTQALNNEKLA 300
+ LLE T++ Q+ DL+E + AKEEAS++E LV S++++LE EEKT+AL+NEK A
Sbjct: 393 IELLEKTIEAQRTDLEEYGRQVCIAKEEASKLENLVESIKSELEISQEEKTRALDNEKAA 452
Query: 301 ASSVQSLLEEKNQLLNELETSKDEEEKSKKAMESLASALHEISTEARETKEKLLSSQADQ 360
S++Q+LL+++ +L ELE K EEEKSKK MESL AL E STE+ E K LL Q +
Sbjct: 453 TSNIQNLLDQRTELSIELERCKVEEEKSKKDMESLTLALQEASTESSEAKATLLVCQEEL 512
Query: 361 ENYESQIENLKLVLKATNEKYENMLENSNHEIDILTSTIEKSKHEYENSKAEWEEKELHL 420
+N ESQ+++LKL K TNEKYE MLE++ +EID L ST++ ++E+ENSKA WE+KELHL
Sbjct: 513 KNCESQVDSLKLASKETNEKYEKMLEDARNEIDSLKSTVDSIQNEFENSKAGWEQKELHL 572
Query: 421 VDAVKKSEEENSSLDKEIDRLVNLLKQTEEEACKMREEEAQLKDSLKEVEAEVIYLQEAL 480
+ VKKSEEENSS +E+ RLVNLLK++EE+AC +EEEA LK++LK E EV YLQE L
Sbjct: 573 MGCVKKSEEENSSSQEEVSRLVNLLKESEEDACARKEEEASLKNNLKVAEGEVKYLQETL 632
Query: 481 GEAKSESMKLKESLLDKENEFQSIHQENEELLTREAASLKKVDELSKLLEEASAKKQTME 540
GEAK+ESMKLKESLLDKE + +++ E L E + L+K++ELSK+ E K+ ++
Sbjct: 633 GEAKAESMKLKESLLDKEEDLKNVTAEISSLREWEGSVLEKIEELSKVKESLVDKETKLQ 692
Query: 541 NGEPTDSE---KDYDLLPKVVEFSEENGKRQEEKTKVEPPIPIEHEEHKFEFPWVG--NG 600
+ E ++ + ++ E S N +E TK++ + + + E ++
Sbjct: 693 SITQEAEELKGREAAHMKQIEELSTANASLVDEATKLQSIVQESEDLKEKEAGYLKKIEE 752
Query: 601 ASDEKTEKTDSAATLQNGNDKPKEAEKKEKEDDSVKVEYKMWESCKIEKKEFSQEG---G 660
S D+ LQ+ + K+ +++E V Y KIE+ + E
Sbjct: 753 LSVANESLADNVTDLQSIVQESKDLKERE-------VAYLK----KIEELSVANESLVDK 812
Query: 661 EPEHESIDDETDS-KPEGGESFDPINGVSSEN---LDDGGHSPSKQQEQQQQQKKKKPLL 715
E + + ID E + + I +S EN +D+ + + +E + ++++ L
Sbjct: 813 ETKLQHIDQEAEELRGREASHLKKIEELSKENENLVDNVANMQNIAEESKDLREREVAYL 839
HSP 2 Score: 171.8 bits (434), Expect = 2.8e-41
Identity = 201/798 (25.19%), Postives = 356/798 (44.61%), Query Frame = 1
Query: 2 EQAGLEEAHKKEEEWEKEIEAVRSQHALDVAALLSTSQELQRVKMELAMTTDAKNQALSH 61
E L+E+ +EE K + A S ++L +EL +VK L + + K Q+++
Sbjct: 610 ESMKLKESLLDKEEDLKNVTAEISSLREWEGSVLEKIEELSKVKESL-VDKETKLQSITQ 669
Query: 62 ADDATKIAEI-HVEKVEILS-------GELTRLKALLDSKLEMQSNENGQL--IMKLKSE 121
+ K E H++++E LS E T+L++++ +++ E G L I +L
Sbjct: 670 EAEELKGREAAHMKQIEELSTANASLVDEATKLQSIVQESEDLKEKEAGYLKKIEELSVA 729
Query: 122 IDSLN---LELEKAKSYAEMVKEKEVSIERLNSELKAAKMAETCYEETIMDKDASIEQLN 181
+SL +L+ ++ +KE+EV+ + EL A E+++DK+ ++ ++
Sbjct: 730 NESLADNVTDLQSIVQESKDLKEREVAYLKKIEELSVAN-------ESLVDKETKLQHID 789
Query: 182 IDLEAAKMAETYAHGLVEEWKNRAEEMETKLDSANKLERSASESLDSVMKQLEHNNDLLH 241
+ E + E +EE ++E E +D+ ++ A ES D +++ + +
Sbjct: 790 QEAEELRGREASHLKKIEEL---SKENENLVDNVANMQNIAEESKDLREREVAYLKKI-- 849
Query: 242 NAELEIA--ALKEKVGLLEMTVKRQKEDLKESEHHLHRAKEEASEMEKLVASLRAQLETV 301
EL A L + V L+ + + ++L+E E L + EE SE+ + + ++L+TV
Sbjct: 850 -DELSTANGTLADNVTNLQ-NISEENKELRERETTLLKKAEELSELNESLVDKASKLQTV 909
Query: 302 NEEKTQALNNEKLAASSVQSLLEEKNQLLNELETSKDEEEKSKKAMESLASALHEISTEA 361
+E + E ++ L + +++L++ ET K+ ++ +A + E
Sbjct: 910 VQENEELRERETAYLKKIEEL-SKLHEILSDQETKLQISNHEKEELKERETAYLKKIEEL 969
Query: 362 RETKEKLLSSQADQENYESQIENLKLVLKATNEKYENMLENSNHEIDILTSTIEKSKHEY 421
+ +E LL+ + + +IE+L+ +K E E
Sbjct: 970 SKVQEDLLNKENELHGMVVEIEDLRSKDSLAQKKIE----------------------EL 1029
Query: 422 ENSKAEWEEKELHLVDAVKKSEEENSSLDKEIDRLVNLLKQTEEEACKMREEEAQLKDSL 481
N A KE L V ++EE LK + K +E + LK SL
Sbjct: 1030 SNFNASLLIKENELQAVVCENEE---------------LKSKQVSTLKTIDELSDLKQSL 1089
Query: 482 ----KEVEAEVIYLQEALGEAK------SESMKLKESLLDKENEFQSIHQENEELLTREA 541
KE++A ++ ++ EA E LK++L+DK+NE Q + ENEEL +EA
Sbjct: 1090 IHKEKELQAAIVENEKLKAEAALSLQRIEELTNLKQTLIDKQNELQGVFHENEELKAKEA 1149
Query: 542 ASLKKVDEL-----SKLLEEASAKKQTMENGEPTDSEKDYDLLPKVVEFS--EENGKRQE 601
+SLKK+DEL S L +E+ ++ T EN E +D K+ E S +E+ +E
Sbjct: 1150 SSLKKIDELLHLEQSWLEKESEFQRVTQENLEL--KTQDALAAKKIEELSKLKESLLEKE 1209
Query: 602 EKTKVEPPIPIEHEEHKFEFPWVGNGASDEKTEKTDSAATLQ-----NGNDKPKEAEKKE 661
+ K +E E + GN + + D + NG++K K ++
Sbjct: 1210 TELKCREAAALEKMEEPSKH---GNSELNSIGKDYDLVQFSEVNGASNGDEKTKTDHYQQ 1269
Query: 662 KEDDSV-----------------------------------KVEYKMWESCKIEKKEFSQ 721
+ + + + E+KMW+S KIEK E S
Sbjct: 1270 RSREHMIQESPMEAIDKHLMGERAAIHKVAHRVEGERNVEKESEFKMWDSYKIEKSEVSP 1329
Query: 722 EGGEPEHESIDDETDSKPEGGESFDPI-NGVS-SENLDDGGHSPSKQQEQQQQQKKKKPL 726
E E E +S+++E DSK E E+ D NG S +++ +D G+ K +Q KKKKPL
Sbjct: 1330 E-RETELDSVEEEVDSKAESSENMDQYSNGFSLTDHTEDSGNLLLK----EQHMKKKKPL 1344
HSP 3 Score: 109.8 bits (273), Expect = 1.3e-22
Identity = 160/672 (23.81%), Postives = 288/672 (42.86%), Query Frame = 1
Query: 11 KKEEEWEKEIEAVRS---QHALDVAALLSTSQELQR----VKMELAMTTDAKNQALSHAD 70
+K E EK IEA R+ ++ V + +L+ +K EL ++ + K +AL +
Sbjct: 363 EKIELLEKTIEAQRTDLEEYGRQVCIAKEEASKLENLVESIKSELEISQEEKTRALDNEK 422
Query: 71 DATKIAEIHVEKVEILSGELTRLKALLD-SKLEMQSNENGQLIMKLKSEIDSLNLELEKA 130
AT + +++ LS EL R K + SK +M+S L + + E +A
Sbjct: 423 AATSNIQNLLDQRTELSIELERCKVEEEKSKKDMES---------LTLALQEASTESSEA 482
Query: 131 KSYAEMVKEKEVSIERLNSELK-AAKMAETCYEETIMDKDASIEQLNIDLEAAK------ 190
K+ + +E+ + E LK A+K YE+ + D I+ L +++ +
Sbjct: 483 KATLLVCQEELKNCESQVDSLKLASKETNEKYEKMLEDARNEIDSLKSTVDSIQNEFENS 542
Query: 191 -----MAETYAHGLVEEWKNRAEEMETKLDSANKLERSASESLDSVMKQLEHNNDLLHN- 250
E + G V K EE + + ++L ES + + E L +N
Sbjct: 543 KAGWEQKELHLMGCV---KKSEEENSSSQEEVSRLVNLLKESEEDACARKEEEASLKNNL 602
Query: 251 --AELEIAALKEKVGLLEMTVKRQKEDLKESEHHLHRAKEEASEMEKLVASLRAQLETVN 310
AE E+ L+E +G + + KE L + E L E S + + S+ ++E ++
Sbjct: 603 KVAEGEVKYLQETLGEAKAESMKLKESLLDKEEDLKNVTAEISSLREWEGSVLEKIEELS 662
Query: 311 EEKTQALNNEKLAASSVQSLLEEKNQLLNELETSKDEEEKSKKAMESL---ASALHEIST 370
+ K ++ E + +QS+ +E +L + E+ A SL A+ L I
Sbjct: 663 KVKESLVDKE----TKLQSITQEAEELKGREAAHMKQIEELSTANASLVDEATKLQSIVQ 722
Query: 371 EARETKEKLLSSQADQENYESQIENLKLVLKATNEKYENMLENSNHEIDILTSTIEKSKH 430
E+ + KEK + Y +IE L + E++ +N + L S +++SK
Sbjct: 723 ESEDLKEK-------EAGYLKKIEELSVA-------NESLADN----VTDLQSIVQESK- 782
Query: 431 EYENSKAEWEEKELHLVDAVKKSEEENSSL-DKEIDRLVNLLKQTEEEACKMREEEAQLK 490
+ +E+E+ + +++ N SL DKE L+ ++EA ++R EA
Sbjct: 783 -------DLKEREVAYLKKIEELSVANESLVDKETK-----LQHIDQEAEELRGREAS-- 842
Query: 491 DSLKEVEAEVIYLQEALGEAKSESMKLKESLLDKENEFQSIHQENEELLTREAASLKKVD 550
LK++E E K E+L+D Q+I +E+++L RE A LKK+D
Sbjct: 843 -HLKKIE---------------ELSKENENLVDNVANMQNIAEESKDLREREVAYLKKID 902
Query: 551 ELSK---LLEEASAKKQTMENGEPTDSEKDYDLLPKVVEFSEENGKRQEEKTKVEPPIPI 610
ELS L + Q + E++ LL K E SE N ++ +K++ +
Sbjct: 903 ELSTANGTLADNVTNLQNISEENKELRERETTLLKKAEELSELNESLVDKASKLQTVVQE 962
Query: 611 EHEEHKFEFPWVGNGASDEKTEK--TDSAATLQNGNDKPKEAEKKEKEDDSVKVEYKMWE 651
E + E ++ K + +D LQ N + +E KE+E +K K+ E
Sbjct: 963 NEELRERETAYLKKIEELSKLHEILSDQETKLQISNHEKEEL--KERETAYLK---KIEE 964
HSP 4 Score: 106.7 bits (265), Expect = 1.1e-21
Identity = 162/732 (22.13%), Postives = 300/732 (40.98%), Query Frame = 1
Query: 4 AGLEEAHKKEEEWEKEIEAVRSQHALDVAALLSTSQELQRVKMELAMTTDAKNQALSHAD 63
+ +EE K E EKE+E + S S+ ++ V +LA ++ S D
Sbjct: 307 SSVEEWKNKVHELEKEVEESNRSKS-------SASESMESVMKQLAELNHVLHETKS--D 366
Query: 64 DATKIAEIHVEKVEILSGELTRLKALLDSKLEMQSNENGQLIMKLKSEIDSLNLELEKAK 123
+A + EK+E+L + + L+ E G+ + K E L +E K
Sbjct: 367 NAAQ-----KEKIELLEKTIEAQRTDLE--------EYGRQVCIAKEEASKLENLVESIK 426
Query: 124 SYAEMVKEKEVSIERLNSELKAAKMAETCYEETIMDKDASIEQLNIDLEAAKM-AETYAH 183
S E+ +E++ L++E A + ++ + +E+ ++ E +K E+
Sbjct: 427 SELEISQEEKT--RALDNEKAATSNIQNLLDQRT-ELSIELERCKVEEEKSKKDMESLTL 486
Query: 184 GLVEEWKNRAEEMETKLDSANKLERSASESLDSVMKQLEHNND----LLHNAELEIAALK 243
L E +E T L +L+ S+ +DS+ + N+ +L +A EI +LK
Sbjct: 487 ALQEASTESSEAKATLLVCQEELKNCESQ-VDSLKLASKETNEKYEKMLEDARNEIDSLK 546
Query: 244 EKVGLLEMTVKRQKEDLKESEHHLHRAKEEASEMEKLVASLRAQLETVNEEKTQALNNEK 303
V ++ + K ++ E HL +++ E + + EE ++ +N K
Sbjct: 547 STVDSIQNEFENSKAGWEQKELHLMGCVKKSEE----------ENSSSQEEVSRLVNLLK 606
Query: 304 LAASSVQSLLEEKNQLLNELETSKDEEEKSKKAMESLASALHEISTEARETKEKLLSSQA 363
+ + EE+ L N L+ ++ E ++ L L E E+ + KE LL +
Sbjct: 607 ESEEDACARKEEEASLKNNLKVAEGE-------VKYLQETLGEAKAESMKLKESLLDKEE 666
Query: 364 DQENYESQIENLKLVLKATNEKYENMLENSNHEIDILTSTIEKSKHEYENSKAEWEEKEL 423
D +N ++I +L+ + EK E + + +D T ++ E E K +E
Sbjct: 667 DLKNVTAEISSLREWEGSVLEKIEELSKVKESLVDKETK-LQSITQEAEELKG----REA 726
Query: 424 HLVDAVKKSEEENSSLDKEIDRLVNLLKQTEEEACKMREEEAQLKDSLKEVEAEVIYLQE 483
+ +++ N+SL E +L ++++++E+ LKE EA + E
Sbjct: 727 AHMKQIEELSTANASLVDEATKLQSIVQESED---------------LKEKEAGYLKKIE 786
Query: 484 ALGEAKSESMKLKESLLDKENEFQSIHQENEELLTREAASLKKVDELSKLLEEASAKKQT 543
L A ESL D + QSI QE+++L RE A LKK++ELS E K+
Sbjct: 787 ELSVAN-------ESLADNVTDLQSIVQESKDLKEREVAYLKKIEELSVANESLVDKETK 846
Query: 544 MENGEPTDSE---KDYDLLPKVVEFSEENGKRQEEKTKVEPPIPIEHEEHKFEFPWVG-- 603
+++ + E ++ L K+ E S+EN + ++ + + E ++
Sbjct: 847 LQHIDQEAEELRGREASHLKKIEELSKENENLVDNVANMQNIAEESKDLREREVAYLKKI 906
Query: 604 NGASDEKTEKTDSAATLQNGNDKPKEAEKKEKEDDSVKVEYKMWESCKIEKKEFSQEGGE 663
+ S D+ LQN +++ KE ++E E ++K Q +
Sbjct: 907 DELSTANGTLADNVTNLQNISEENKELRERETTLLKKAEELSELNESLVDKASKLQTVVQ 966
Query: 664 PEHESIDDETD--SKPEGGESFDPINGVSSENLDDGGHSPSKQQEQQQQQKKK-KPLLKK 723
E + ET K E I L H + +E++ KK + L K
Sbjct: 967 ENEELRERETAYLKKIEELSKLHEILSDQETKLQISNHEKEELKERETAYLKKIEELSKV 968
HSP 5 Score: 102.4 bits (254), Expect = 2.1e-20
Identity = 143/657 (21.77%), Postives = 281/657 (42.77%), Query Frame = 1
Query: 6 LEEAHKKEEEWEKEIEAVR-------SQHALDVAALLSTSQELQRVKMEL---------- 65
LE +EE+ +K++E++ ++ + A LL +EL+ + ++
Sbjct: 442 LERCKVEEEKSKKDMESLTLALQEASTESSEAKATLLVCQEELKNCESQVDSLKLASKET 501
Query: 66 -----AMTTDAKNQALSHADDATKIA-EIHVEKVEILSGELTRLKALLDSKLEMQSNEN- 125
M DA+N+ S I E K EL + + S+ E S++
Sbjct: 502 NEKYEKMLEDARNEIDSLKSTVDSIQNEFENSKAGWEQKELHLMGCVKKSEEENSSSQEE 561
Query: 126 -GQLIMKLKSEIDSLNLELEKAKSYAEMVKEKEVSIERLNSELKAAKMAETCYEETIMDK 185
+L+ LK + E+ S +K E ++ L L AK +E+++DK
Sbjct: 562 VSRLVNLLKESEEDACARKEEEASLKNNLKVAEGEVKYLQETLGEAKAESMKLKESLLDK 621
Query: 186 DASIEQLNIDLEAAKMAETYAHGLVEEWKNRAEEMETKLDSANKLERSASESLDSVMKQL 245
+ ++ N+ E + + E + ++E+ + ++ E+ +D KL+ E+ + ++
Sbjct: 622 EEDLK--NVTAEISSLRE-WEGSVLEKIEELSKVKESLVDKETKLQSITQEAEELKGREA 681
Query: 246 EHNNDLLHNAELEIAALKEKVGLLEMTVKRQKEDLKESEHHLHRAKEEASEMEKLVASLR 305
H + + + + E L ++ ++ EDLKE E + EE S + +A
Sbjct: 682 AHMKQIEELSTANASLVDEATKL--QSIVQESEDLKEKEAGYLKKIEELSVANESLADNV 741
Query: 306 AQLETVNEEKTQALNNEKLAASSVQSLLEEKNQLLNELETSKDEEEKSKKAMESLASALH 365
L+++ +E E ++ L L+++ + ++++++ AS L
Sbjct: 742 TDLQSIVQESKDLKEREVAYLKKIEELSVANESLVDKETKLQHIDQEAEELRGREASHLK 801
Query: 366 EISTEARETKEKLLSSQADQENYESQIENLKLVLKATNEKYENMLENSNHEIDILTSTIE 425
+I ++E E L+ + A+ +N + ++L+ A +K + + + D +T+
Sbjct: 802 KIEELSKEN-ENLVDNVANMQNIAEESKDLREREVAYLKKIDELSTANGTLADNVTNLQN 861
Query: 426 KSKHEYENSKAEWEEKELHLVDAVKKSEEENSSLDKEIDRLVNLLKQTEEEACKMREEEA 485
S+ EN E E+E L+ ++ E N SL + +L ++++ EE +RE E
Sbjct: 862 ISE---ENK--ELRERETTLLKKAEELSELNESLVDKASKLQTVVQENEE----LRERET 921
Query: 486 QLKDSLKEVEAEVIYLQEALGEAKSESMKLKESLLDKENEFQSIHQENEELLTREAASLK 545
LK++E E KL E L D+E + Q + E EEL RE A LK
Sbjct: 922 AY---LKKIE---------------ELSKLHEILSDQETKLQISNHEKEELKERETAYLK 981
Query: 546 KVDELSKLLEEASAKKQTMENGEPTDSE----KDYDLLPKVVEFSEENGKRQEEKTKVEP 605
K++ELSK+ E+ K+ + +G + E KD K+ E S N ++ +++
Sbjct: 982 KIEELSKVQEDLLNKENEL-HGMVVEIEDLRSKDSLAQKKIEELSNFNASLLIKENELQ- 1041
Query: 606 PIPIEHEEHKFEFPWVGNGASDEKTEKTDSAATLQNGNDKPKEAEKKEKEDDSVKVE 634
+ E+EE K+++ + T+ +D + KEKE + VE
Sbjct: 1042 AVVCENEE--------------LKSKQVSTLKTIDELSDLKQSLIHKEKELQAAIVE 1049
HSP 6 Score: 77.8 bits (190), Expect = 5.5e-13
Identity = 105/461 (22.78%), Postives = 196/461 (42.52%), Query Frame = 1
Query: 227 LHNAELEIAALKEKVGLLEMTVKRQKEDLKESEHHLHRAKEEASEMEKLVASLRAQLETV 286
L+ + ++ E++ LL+ + +DLKESE + A E+ E L A RA+ E+
Sbjct: 87 LNQIQEDLKKADEQIELLKKDKAKAIDDLKESEKLVEEANEKLK--EALAAQKRAE-ESF 146
Query: 287 NEEKTQALNNEKLAASSVQSL-LEEKNQL----------LNELETSKDEEEKSKKAMESL 346
EK +A+ E+ +VQ + KN+L ++ L ++ +E ++ K +
Sbjct: 147 EVEKFRAVELEQAGLEAVQKKDVTSKNELESIRSQHALDISALLSTTEELQRVKHELSMT 206
Query: 347 ASALHEISTEARETKEKLLSSQADQENYESQIENLKLVLKATNEKY----ENMLENSNHE 406
A A ++ + A E + E S++ LK +L + EK ++ E
Sbjct: 207 ADAKNKALSHAEEATKIAEIHAEKAEILASELGRLKALLGSKEEKEAIEGNEIVSKLKSE 266
Query: 407 IDILTSTIEKSKHEYENSKAEWEEKELHLVDAVKKSEEENSSLDKEIDRLVNLLKQTEEE 466
I++L +EK E+S E E L ++ ++ S + ++ N + + E+E
Sbjct: 267 IELLRGELEKVS-ILESSLKEQEGLVEQLKVDLEAAKMAESCTNSSVEEWKNKVHELEKE 326
Query: 467 ACKMREEEAQLKDSLKEVEAEVIYLQEALGEAKSESMKLKESLLDKENEFQSIHQENEE- 526
+ ++ +S++ V ++ L L E KS++ KE + E ++ + EE
Sbjct: 327 VEESNRSKSSASESMESVMKQLAELNHVLHETKSDNAAQKEKIELLEKTIEAQRTDLEEY 386
Query: 527 -----LLTREAASLKK-VDELSKLLEEASAKKQTMENGEPTDSEKDYDLLPKVVEFSEEN 586
+ EA+ L+ V+ + LE + +K + E + +LL + E S E
Sbjct: 387 GRQVCIAKEEASKLENLVESIKSELEISQEEKTRALDNEKAATSNIQNLLDQRTELSIE- 446
Query: 587 GKRQEEKTKVEPPIPIEHEEHKFEFPWVGNGASDEKTEKTDSAATLQNGNDKPKEAEKKE 646
E+ KV E E+ K + + + TE +++ ATL ++ K E
Sbjct: 447 ----LERCKV------EEEKSKKDMESLTLALQEASTESSEAKATLLVCQEELKNC---E 506
Query: 647 KEDDSVKVEYKMWESCKIEKKEFSQEGGEPEHESIDDETDS 666
+ DS+K+ K EK E E E +S+ DS
Sbjct: 507 SQVDSLKLASKETN----EKYEKMLEDARNEIDSLKSTVDS 525
BLAST of Cucsa.318540 vs. Swiss-Prot
Match:
Y4759_ARATH (WEB family protein At4g27595, chloroplastic OS=Arabidopsis thaliana GN=At4g27595 PE=2 SV=1)
HSP 1 Score: 511.1 bits (1315), Expect = 1.9e-143
Identity = 309/731 (42.27%), Postives = 454/731 (62.11%), Query Frame = 1
Query: 1 MEQAGLEEAHKKEEEWEKEIEAVRSQHALDVAALLSTSQELQRVKMELAMTTDAKNQALS 60
+EQAG+E HKKE W+KE+E++RSQHALD++ALLST++EL R+K ELAMT DAKN+ALS
Sbjct: 165 LEQAGIEAVHKKEVSWKKEVESIRSQHALDISALLSTTEELHRIKQELAMTADAKNKALS 224
Query: 61 HADDATKIAEIHVEKVEILSGELTRLKALLDSKLEMQSNENGQLIMKLKSEIDSLNLELE 120
HA++ATKIAE EK EILS EL+RLKAL+ S + +SNE+ +++ KLKSEI+ L +LE
Sbjct: 225 HAEEATKIAENQAEKAEILSSELSRLKALVGSDEQKKSNEDDEVVSKLKSEIEMLRGKLE 284
Query: 121 KAKSYAEMVKEKEVSIERLNSELKAAKMAETCYEETIMDKDASIEQLNIDLEAAKMAETY 180
K VSI E T+ D++ SIE L++DL+AAKM E+Y
Sbjct: 285 K------------VSI----------------LENTLKDQEESIELLHVDLQAAKMVESY 344
Query: 181 AHGLVEEWKNRAEEMETKLDSANKLERSASESLDSVMKQLEHNNDLLHNAELEIAALKEK 240
A+ L EWKN E++ +++ + +L+ SASESLD MKQLE NN LH AEL A LKEK
Sbjct: 345 ANNLAAEWKN---EVDKQVEESKELKTSASESLDLAMKQLEENNHALHEAELGNATLKEK 404
Query: 241 VGLLEMTVKRQKEDLKESEHHLHRAKEEASEMEKLVASLRAQLETVNEEKTQALNNEKLA 300
V L T+ RQ+ DL+ES+H + +KEE S++EKLV S+++ LET EK +AL NEK A
Sbjct: 405 VESLVTTIGRQENDLEESQHQVCISKEETSKLEKLVESIKSDLETTQGEKVRALLNEKTA 464
Query: 301 ASSVQSLLEEKNQLLNELETSKDEEEKSKKAMESLASALHEISTEARETKEKLLSSQADQ 360
S +Q+LL EK +L ELE K EEEK KKAMESL L E+S EA+E KEKLL+ QA+
Sbjct: 465 TSQIQNLLSEKTELATELENCKKEEEKIKKAMESLTLDLQEVSVEAKEAKEKLLTCQAEL 524
Query: 361 ENYESQIENLKLVLKATNEKYENMLENSNHEIDILTSTIEKSKHEYENSKAEWEEKELHL 420
E QIE+LKL K TNEK+ MLE++ +EID L S++E +++E+ NSK EWE++ELHL
Sbjct: 525 ELCGVQIESLKLAEKDTNEKHGKMLEDARNEIDGLKSSLENTENEFFNSKTEWEQRELHL 584
Query: 421 VDAVKKSEEENSSLDKEIDRLVNLLKQTEEEACKMREEEAQLKDSLKEVEAEVIYLQEAL 480
+ VKK E+ N S+ +E+ ++ NLL E EAC +EE+A+++ + KE+E E+ LQE +
Sbjct: 585 MLCVKKLEDGNFSVQEELSKVKNLLHLKEVEACAAKEEDAKMQTNRKELEEEIKDLQEIV 644
Query: 481 GEAKSESMKLKESLLDKENEFQSIHQENEELLTREAASLKKVDELSKLLEEASAKKQTME 540
AK++SMKLKESL++KE+E ++ EN +L E +S+ K+D+LSK+ E K+ ++
Sbjct: 645 EVAKADSMKLKESLVEKEDELKNTAAENRKLREMEVSSIDKIDQLSKVKESLVDKETKLQ 704
Query: 541 NGEPTDSE---KDYDLLPKVVEFSEENGKRQEEKTKVEPPIPIEHEEHKFEFPWVG--NG 600
N E K+ D L K+ E S E++TK+ + E + E +
Sbjct: 705 NIIQEAEELRVKEIDYLKKIEELSAAKESLVEKETKLLSTVQEAEELRRRELACLKKIEE 764
Query: 601 ASDEKTEKTDSAATLQNGNDKPKEAEKKEKEDDSVK-VEYKMWESCKIEKKEFSQEGGEP 660
S D LQ+ + + KE+E +++K +E + ++ +KE +
Sbjct: 765 LSAVNERLVDKETKLQSSIQEVEVL--KEREAENIKQIEELSLSNERLVEKEAKLQTVVQ 824
Query: 661 EHESIDDETDSKPEGGESFDPINGVSSENLDDGGHSPSKQQEQQQQQKKKKPLLKKFGYL 720
E+E + ++ + + E ++ + + D S QE ++ ++++ LKK L
Sbjct: 825 ENEELREKESAYQKKIEELSKVDEIFA---DREAKLQSSTQENEELREREVAYLKKIEEL 859
Query: 721 LKKKNSVNQKQ 726
K + ++ K+
Sbjct: 885 AKLQENLLDKE 859
HSP 2 Score: 170.6 bits (431), Expect = 6.2e-41
Identity = 192/728 (26.37%), Postives = 329/728 (45.19%), Query Frame = 1
Query: 18 KEIEAVRSQHALDVAALLSTSQELQRVKMELAMTTDAKNQALSHADDATKIAEIHVEKVE 77
KE+EA ++ + A + + +EL+ +L + D+ K+ E VEK +
Sbjct: 581 KEVEACAAKE--EDAKMQTNRKELEEEIKDLQEIVEVAKA------DSMKLKESLVEKED 640
Query: 78 ILSGELTRLKALLDSKLEMQSNENGQLIMKLKSEIDSLNLELEKAKSYAEMVKEKEVSIE 137
L + L + +E+ S + + K+K + +L+ AE ++ KE+
Sbjct: 641 ELKNTAAENRKLRE--MEVSSIDKIDQLSKVKESLVDKETKLQNIIQEAEELRVKEIDYL 700
Query: 138 RLNSELKAAKMAETCYEETIMDKDASIEQLNIDLEAAKMAETYAHGLVEEWKNRAEEMET 197
+ EL AAK E++++K+ + + E + E +EE E +
Sbjct: 701 KKIEELSAAK-------ESLVEKETKLLSTVQEAEELRRRELACLKKIEELSAVNERL-- 760
Query: 198 KLDSANKLERSASESLDSVMKQLEHNN-DLLHNAELEIAALKEKVGLLEMTVKRQKEDLK 257
+D KL+ S E V+K+ E N + L L EK L+ TV ++ E+L+
Sbjct: 761 -VDKETKLQSSIQEV--EVLKEREAENIKQIEELSLSNERLVEKEAKLQ-TVVQENEELR 820
Query: 258 ESEHHLHRAKEEASEMEKLVASLRAQLETVNEEKTQALNNEKLAASSVQSLLEEKNQLLN 317
E E + EE S+++++ A A+L++ +E + E ++ L + + LL+
Sbjct: 821 EKESAYQKKIEELSKVDEIFADREAKLQSSTQENEELREREVAYLKKIEELAKLQENLLD 880
Query: 318 ELETSKDE--EEKSKKAMESLASALHEISTEARETKEKLLSSQADQENYESQIENLK--- 377
+ D E + KA +SLA E E + LL +++ ++ + E LK
Sbjct: 881 KENELHDMVLEIEDLKAKDSLAEKKIE---ELSNLNKSLLVKESELQDVVFENEKLKSKE 940
Query: 378 -LVLKATNEKYENMLENSNHEIDILTSTIEKSK------------HEYENSKAEWEEKEL 437
L LK T E + ++ E ++ T+ +E K E +N K +KE
Sbjct: 941 ALSLKTTEELSDVKQTLADKEKELKTAVVENEKLKAQAASSFQKIEELKNLKQSLLDKEN 1000
Query: 438 HLVDAVKKSEE----ENSSLDKEIDRLVNLLKQTEEEACKMREEEAQLKDSLKEVEAEVI 497
L + +EE E SSL K+ID L++L + ++ + +E + + + K +E
Sbjct: 1001 ELEGVFQANEELKAKEASSL-KKIDELLHLEQSWIDKGNENQELKVREASAAKRIE---- 1060
Query: 498 YLQEALGEAKSESMKLKESLLDKENEFQSIHQENEELLTREAASLKKVDELSKLLEEASA 557
E K+KESLLDKE Q++ +N EL REA++LKK++ELSKLLEEAS+
Sbjct: 1061 -----------ELSKMKESLLDKE--LQTVIHDNYELKAREASALKKIEELSKLLEEASS 1120
Query: 558 KKQTMENGEPTDSEKDYDLLPKVVEFSEENGKRQEEKTKVEPPIPIEHEEHKFEFPWVGN 617
T E GE + +D G+++ +++ +E
Sbjct: 1121 ---THEKGEEITNTNPFD---------NSTGEQKVQESPLE------------------- 1180
Query: 618 GASDEKTEKTDSAATLQNGNDKPKEAEKKEKEDDSVKVEYKMWESCKIEKKEFSQEGGEP 677
+ ++ K D+ N + +K K+ D+V+ E E + + +
Sbjct: 1181 --AIDRHLKDDTTIHWSAHNVQVIGKGEKGKDKDTVESEVYHLEKREASSER------DT 1218
Query: 678 EHESIDDETDSKPEGGESFDPI-NGVS-SENLDDGGHSPSKQQEQQQQQKKKKPLLKKFG 721
EH+ ++E DSK EG E+FD + NG+S +E +D + QQQKKKKPLL+KFG
Sbjct: 1241 EHDFAEEEVDSKAEGSENFDQLSNGLSLAEQTED-------VVSKDQQQKKKKPLLRKFG 1218
HSP 3 Score: 116.7 bits (291), Expect = 1.1e-24
Identity = 181/740 (24.46%), Postives = 308/740 (41.62%), Query Frame = 1
Query: 17 EKEIEAVRSQHALDVAALLSTSQELQRVKMELAMTTDAKNQALSHADDATKIAEIHVEKV 76
E ++E + Q + + ++ +K +L T K +AL + AT ++
Sbjct: 385 ENDLEESQHQVCISKEETSKLEKLVESIKSDLETTQGEKVRALLNEKTATS-------QI 444
Query: 77 EILSGELTRLKALLDSKLEMQSNENGQLIMKLKSEIDSLNLELEKAKSYAEMVKEK---- 136
+ L E T L L++ + + K+K ++SL L+L++ A+ KEK
Sbjct: 445 QNLLSEKTELATELENCKKEEE--------KIKKAMESLTLDLQEVSVEAKEAKEKLLTC 504
Query: 137 EVSIERLNSELKAAKMAETCYEET----IMDKDASIEQLNIDLEAAKMAETYAHGLVEEW 196
+ +E ++++ K+AE E + D I+ L LE E EW
Sbjct: 505 QAELELCGVQIESLKLAEKDTNEKHGKMLEDARNEIDGLKSSLENT---ENEFFNSKTEW 564
Query: 197 KNRAEEMETKLDSANKLERSASESLDSVMKQLEHNNDLLHNAELEIAALKEKVGLLEMTV 256
+ R E+ L KLE + SV ++L +LLH E+E A KE+ ++
Sbjct: 565 EQR--ELHLML-CVKKLE----DGNFSVQEELSKVKNLLHLKEVEACAAKEEDAKMQTNR 624
Query: 257 KRQKEDLKESEHHLHRAKEEASEMEKLVASLRAQLETVNEEKTQALNNEKLAASSVQSLL 316
K +E++K+ + + AK ++ ++++ + +E +E K A N KL V S+
Sbjct: 625 KELEEEIKDLQEIVEVAKADSMKLKESL------VEKEDELKNTAAENRKLREMEVSSI- 684
Query: 317 EEKNQLLNELETSKDEEEKSKKAMESLASALHEISTEARETKEKLLSSQADQENYESQIE 376
++ +QL E+ D+E K L I EA E + K + +Y +IE
Sbjct: 685 DKIDQLSKVKESLVDKETK-----------LQNIIQEAEELRVKEI-------DYLKKIE 744
Query: 377 NLKLVLKATNEKYENMLENSNHEIDILTSTIEKSKHEYENSKAEWEEKELHLVDAVKKSE 436
L ++ EK +L ST+++++ E +EL + +++
Sbjct: 745 ELSAAKESLVEKETKLL-----------STVQEAE--------ELRRRELACLKKIEELS 804
Query: 437 EENSSL-DKEID-----RLVNLLKQTEEEACKMREEEAQLKDSLKEVEAE---VIYLQEA 496
N L DKE + V +LK+ E E K EE + + L E EA+ V+ E
Sbjct: 805 AVNERLVDKETKLQSSIQEVEVLKEREAENIKQIEELSLSNERLVEKEAKLQTVVQENEE 864
Query: 497 LGEAKS-------ESMKLKESLLDKENEFQSIHQENEELLTREAASLKKVDELSKLLEEA 556
L E +S E K+ E D+E + QS QENEEL RE A LKK++EL+KL E
Sbjct: 865 LREKESAYQKKIEELSKVDEIFADREAKLQSSTQENEELREREVAYLKKIEELAKLQENL 924
Query: 557 SAKKQTMENG--EPTDSEKDYDLLPKVVEFSEENGKRQEEKTKVEPPIPIEHEEHKFEFP 616
K+ + + E D + L K +E K K + E+E+ K +
Sbjct: 925 LDKENELHDMVLEIEDLKAKDSLAEKKIEELSNLNKSLLVKESELQDVVFENEKLKSK-- 984
Query: 617 WVGNGASDEKTEK-TDSAATLQNGNDKPKEAEKKEKEDDSVKVEYKMWESCKIEKKEFSQ 676
S + TE+ +D TL DK KE + E++ +K + E K Q
Sbjct: 985 ---EALSLKTTEELSDVKQTLA---DKEKELKTAVVENEKLKAQAASSFQKIEELKNLKQ 1042
Query: 677 EGGEPEHESIDDETDSKPEGGESFDPINGVSSENLDDGGHSP----SKQQEQQQQQKKKK 726
+ E +E + + E S + +D+ H K E Q+ + ++
Sbjct: 1045 SLLDKE-----NELEGVFQANEELKAKEASSLKKIDELLHLEQSWIDKGNENQELKVREA 1042
HSP 4 Score: 94.0 bits (232), Expect = 7.4e-18
Identity = 172/731 (23.53%), Postives = 302/731 (41.31%), Query Frame = 1
Query: 9 AHKKEEEWEKEIEAVRSQHALDVAALLSTSQELQRVKMELAMTTDAKNQALSHADDATKI 68
A+ EW+ E++ + + + ++ S S +L ++E N AL A+
Sbjct: 317 ANNLAAEWKNEVDK-QVEESKELKTSASESLDLAMKQLE------ENNHALHEAELGNAT 376
Query: 69 AEIHVEKVEILSGELTRLKALLDSKLEMQ--SNENGQLIMKLKSEIDSLNLELEKAKSYA 128
+ EKVE L + R + L+ S E + KL I S +LE + +
Sbjct: 377 LK---EKVESLVTTIGRQENDLEESQHQVCISKEETSKLEKLVESIKS-DLETTQGEKVR 436
Query: 129 EMVKEKEVS--IERLNSE-LKAAKMAETCYEETIMDKDASIEQLNIDLEAAKMAETYAHG 188
++ EK + I+ L SE + A E C +E K A +E L +DL+ + A
Sbjct: 437 ALLNEKTATSQIQNLLSEKTELATELENCKKEEEKIKKA-MESLTLDLQEVSVEAKEAKE 496
Query: 189 LVEEWKNRAEEMETKLDSANKLERSASESLDSVMKQLEHNNDLLHNAELEIAALKEKVGL 248
+ + E +++S E+ +E + +L +A EI LK
Sbjct: 497 KLLTCQAELELCGVQIESLKLAEKDTNEK----------HGKMLEDARNEIDGLKSS--- 556
Query: 249 LEMTVKRQKEDLKESEHHLHRAKEEASEMEKLVASLRAQLETVNEEKTQALNNEKLAASS 308
L+ +E+ +K E + E + +LE N + L S
Sbjct: 557 -----------LENTENEFFNSKTEWEQRELHLMLCVKKLEDGNFSVQEEL-------SK 616
Query: 309 VQSLLEEKNQLLNELETSKDEEEKSK-----KAMESLASALHEISTEARETKEKLLSSQA 368
V++LL L E+E +EE +K K +E L EI A+ KL S
Sbjct: 617 VKNLLH-----LKEVEACAAKEEDAKMQTNRKELEEEIKDLQEIVEVAKADSMKLKESLV 676
Query: 369 DQENYESQIENLKLVLKATNEKYENMLENSNHEIDILTSTIEK---SKHEYENSKAEWEE 428
++E+ +++N A N K M +S +ID L+ E + + +N E EE
Sbjct: 677 EKED---ELKNTA----AENRKLREMEVSSIDKIDQLSKVKESLVDKETKLQNIIQEAEE 736
Query: 429 KELHLVDAVKKSEEENSSLDKEIDRLVNLLKQTEEEACKMREEEAQLKDSLKEVEAEVIY 488
+ +D +KK EE +++ + +++ LL T +EA ++R E ++E+ A
Sbjct: 737 LRVKEIDYLKKIEELSAAKESLVEKETKLLS-TVQEAEELRRRELACLKKIEELSA---- 796
Query: 489 LQEALGEAKSESMKLKESLLDKENEFQSIHQENEELLTREAASLKKVDELS---KLLEEA 548
+ E L+DKE + QS QE E L REA ++K+++ELS + L E
Sbjct: 797 --------------VNERLVDKETKLQSSIQEVEVLKEREAENIKQIEELSLSNERLVEK 856
Query: 549 SAKKQTM--ENGEPTDSEKDY----DLLPKVVE-FSEENGKRQEEKTKVEP--PIPIEHE 608
AK QT+ EN E + E Y + L KV E F++ K Q + E + +
Sbjct: 857 EAKLQTVVQENEELREKESAYQKKIEELSKVDEIFADREAKLQSSTQENEELREREVAYL 916
Query: 609 EHKFEFPWVGNGASDEKTEKTDSAATLQNGNDKPKEAEKKEKEDDSVKVEYKMWESCKIE 668
+ E + D++ E D +++ K AEKK +E ++ + ES +++
Sbjct: 917 KKIEELAKLQENLLDKENELHDMVLEIEDLKAKDSLAEKKIEELSNLNKSLLVKES-ELQ 972
Query: 669 KKEFSQEGGEPEH----ESIDDETDSKPEGGESFDPINGVSSENLDDGGHSPSKQQEQQQ 711
F E + + ++ ++ +D K + + EN + S Q+ ++
Sbjct: 977 DVVFENEKLKSKEALSLKTTEELSDVKQTLADKEKELKTAVVENEKLKAQAASSFQKIEE 972
BLAST of Cucsa.318540 vs. Swiss-Prot
Match:
USO1_YEAST (Intracellular protein transport protein USO1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=USO1 PE=1 SV=2)
HSP 1 Score: 139.4 bits (350), Expect = 1.5e-31
Identity = 177/757 (23.38%), Postives = 339/757 (44.78%), Query Frame = 1
Query: 12 KEEEWEKEIEAVRSQHALDVAALLSTSQELQRVKMELAMTTDAKNQA-LSHADDATKIAE 71
+ +E +++ + + S H+ EL+ V+ L T ++ ++ T + E
Sbjct: 770 EHKELDEKYQILNSSHSSLKENFSILETELKNVRDSLDEMTQLRDVLETKDKENQTALLE 829
Query: 72 ----IHVEKVEILSGELTRLKALLDSKLEMQS--NENGQLIMKLKSEIDSL-----NLEL 131
IH ++ I + E L+ +L K + + N+ G+ + L E+ ++ NL+
Sbjct: 830 YKSTIHKQEDSIKTLE-KGLETILSQKKKAEDGINKMGKDLFALSREMQAVEENCKNLQK 889
Query: 132 EKAKSYAEMVKEKEVSIERLNSELKAAK-MAETCYEETIMDKDASIEQLNIDLE------ 191
EK KS KE + E + +++ K + E E I + S E+ +I E
Sbjct: 890 EKDKSNVNHQKETKSLKEDIAAKITEIKAINENLEEMKIQCNNLSKEKEHISKELVEYKS 949
Query: 192 --------AAKMAETYAHGLVEEWKNRAEEMETKLDSANKLERSASESLDSVMKQLEHNN 251
AK+ E L +K+ E E+ + + + + +S L ++ +++ +
Sbjct: 950 RFQSHDNLVAKLTEKLK-SLANNYKDMQAENESLIKAVEESKNESSIQLSNLQNKIDSMS 1009
Query: 252 DLLHNAELEIAALKEKVGLLEMTVKRQKEDLKES-EHHLHRAKEEASEMEKLVASLRAQL 311
N ++E ++++ + L+ T+ DL+++ E + ++ E E ++ L+ +L
Sbjct: 1010 QEKENFQIERGSIEKNIEQLKKTIS----DLEQTKEEIISKSDSSKDEYESQISLLKEKL 1069
Query: 312 ETVNEEKTQALNNEKLAASSVQSLLEE-------KNQLLNELETSKDEEEKSKKAMESLA 371
ET + +N + + L E KN+L +LETS+ ++ K+ E L
Sbjct: 1070 ETATTANDENVNKISELTKTREELEAELAAYKNLKNELETKLETSEKALKEVKENEEHLK 1129
Query: 372 SALHEISTEARETKEKLLSSQADQENYESQIENLKLVLKATNEKYENMLENSNHEIDILT 431
++ EA ETK++L S +A+ E+ E + E+L LK E+ N N EI L
Sbjct: 1130 EEKIQLEKEATETKQQLNSLRANLESLEKEHEDLAAQLKKYEEQIANKERQYNEEISQLN 1189
Query: 432 STIEKSKHEYENSKAEWEEKELHLVDAVKKSEEENSSLDK-EIDRLVNLLKQTEEEACKM 491
I ++ E E+ K + +E E V A+K + EE S+L K EID L NL Q +E K
Sbjct: 1190 DEITSTQQENESIKKKNDELEGE-VKAMKSTSEEQSNLKKSEIDAL-NL--QIKELKKKN 1249
Query: 492 REEEAQLKDSLKEVEAEVIYLQEALGEAKSESMKLKESLLDKENEFQSIHQENEELLTRE 551
EA L +S+K VE+E + ++E E + ++ E E++ ++ +N + L +
Sbjct: 1250 ETNEASLLESIKSVESETVKIKELQDECNFKEKEVSE----LEDKLKASEDKNSKYLELQ 1309
Query: 552 AASLKKVDELSKLLEEASAKKQTMENGEPTDSEKDYDLLPKVVEFSEENGKRQEEKTKVE 611
S K +EL E + + + N + + +L SEE +E+ K++
Sbjct: 1310 KESEKIKEELDAKTTELKIQLEKITNLSKAKEKSESELSRLKKTSSEERKNAEEQLEKLK 1369
Query: 612 PPIPIEHEEHKFEFPWVGNGASDEKTEKTDSAATLQNGNDK-PKEAEKKEKEDDSVKVEY 671
I I+++ + E + G+S E ++ TL++ + E E K KE D+ + E
Sbjct: 1370 NEIQIKNQAFEKERKLLNEGSSTITQEYSEKINTLEDELIRLQNENELKAKEIDNTRSEL 1429
Query: 672 K---MWESCKIEKKEFSQEGGEPEHESIDDETDSKPEGGESFDPINGVSSENLDD----G 721
+ + +E+K+ + + + E S D+ E S + N E+L +
Sbjct: 1430 EKVSLSNDELLEEKQNTIKSLQDEILSYKDKITRNDEKLLSIERDNKRDLESLKEQLRAA 1489
BLAST of Cucsa.318540 vs. TrEMBL
Match:
A0A0A0LRJ0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G442260 PE=4 SV=1)
HSP 1 Score: 1396.3 bits (3613), Expect = 0.0e+00
Identity = 725/725 (100.00%), Postives = 725/725 (100.00%), Query Frame = 1
Query: 1 MEQAGLEEAHKKEEEWEKEIEAVRSQHALDVAALLSTSQELQRVKMELAMTTDAKNQALS 60
MEQAGLEEAHKKEEEWEKEIEAVRSQHALDVAALLSTSQELQRVKMELAMTTDAKNQALS
Sbjct: 155 MEQAGLEEAHKKEEEWEKEIEAVRSQHALDVAALLSTSQELQRVKMELAMTTDAKNQALS 214
Query: 61 HADDATKIAEIHVEKVEILSGELTRLKALLDSKLEMQSNENGQLIMKLKSEIDSLNLELE 120
HADDATKIAEIHVEKVEILSGELTRLKALLDSKLEMQSNENGQLIMKLKSEIDSLNLELE
Sbjct: 215 HADDATKIAEIHVEKVEILSGELTRLKALLDSKLEMQSNENGQLIMKLKSEIDSLNLELE 274
Query: 121 KAKSYAEMVKEKEVSIERLNSELKAAKMAETCYEETIMDKDASIEQLNIDLEAAKMAETY 180
KAKSYAEMVKEKEVSIERLNSELKAAKMAETCYEETIMDKDASIEQLNIDLEAAKMAETY
Sbjct: 275 KAKSYAEMVKEKEVSIERLNSELKAAKMAETCYEETIMDKDASIEQLNIDLEAAKMAETY 334
Query: 181 AHGLVEEWKNRAEEMETKLDSANKLERSASESLDSVMKQLEHNNDLLHNAELEIAALKEK 240
AHGLVEEWKNRAEEMETKLDSANKLERSASESLDSVMKQLEHNNDLLHNAELEIAALKEK
Sbjct: 335 AHGLVEEWKNRAEEMETKLDSANKLERSASESLDSVMKQLEHNNDLLHNAELEIAALKEK 394
Query: 241 VGLLEMTVKRQKEDLKESEHHLHRAKEEASEMEKLVASLRAQLETVNEEKTQALNNEKLA 300
VGLLEMTVKRQKEDLKESEHHLHRAKEEASEMEKLVASLRAQLETVNEEKTQALNNEKLA
Sbjct: 395 VGLLEMTVKRQKEDLKESEHHLHRAKEEASEMEKLVASLRAQLETVNEEKTQALNNEKLA 454
Query: 301 ASSVQSLLEEKNQLLNELETSKDEEEKSKKAMESLASALHEISTEARETKEKLLSSQADQ 360
ASSVQSLLEEKNQLLNELETSKDEEEKSKKAMESLASALHEISTEARETKEKLLSSQADQ
Sbjct: 455 ASSVQSLLEEKNQLLNELETSKDEEEKSKKAMESLASALHEISTEARETKEKLLSSQADQ 514
Query: 361 ENYESQIENLKLVLKATNEKYENMLENSNHEIDILTSTIEKSKHEYENSKAEWEEKELHL 420
ENYESQIENLKLVLKATNEKYENMLENSNHEIDILTSTIEKSKHEYENSKAEWEEKELHL
Sbjct: 515 ENYESQIENLKLVLKATNEKYENMLENSNHEIDILTSTIEKSKHEYENSKAEWEEKELHL 574
Query: 421 VDAVKKSEEENSSLDKEIDRLVNLLKQTEEEACKMREEEAQLKDSLKEVEAEVIYLQEAL 480
VDAVKKSEEENSSLDKEIDRLVNLLKQTEEEACKMREEEAQLKDSLKEVEAEVIYLQEAL
Sbjct: 575 VDAVKKSEEENSSLDKEIDRLVNLLKQTEEEACKMREEEAQLKDSLKEVEAEVIYLQEAL 634
Query: 481 GEAKSESMKLKESLLDKENEFQSIHQENEELLTREAASLKKVDELSKLLEEASAKKQTME 540
GEAKSESMKLKESLLDKENEFQSIHQENEELLTREAASLKKVDELSKLLEEASAKKQTME
Sbjct: 635 GEAKSESMKLKESLLDKENEFQSIHQENEELLTREAASLKKVDELSKLLEEASAKKQTME 694
Query: 541 NGEPTDSEKDYDLLPKVVEFSEENGKRQEEKTKVEPPIPIEHEEHKFEFPWVGNGASDEK 600
NGEPTDSEKDYDLLPKVVEFSEENGKRQEEKTKVEPPIPIEHEEHKFEFPWVGNGASDEK
Sbjct: 695 NGEPTDSEKDYDLLPKVVEFSEENGKRQEEKTKVEPPIPIEHEEHKFEFPWVGNGASDEK 754
Query: 601 TEKTDSAATLQNGNDKPKEAEKKEKEDDSVKVEYKMWESCKIEKKEFSQEGGEPEHESID 660
TEKTDSAATLQNGNDKPKEAEKKEKEDDSVKVEYKMWESCKIEKKEFSQEGGEPEHESID
Sbjct: 755 TEKTDSAATLQNGNDKPKEAEKKEKEDDSVKVEYKMWESCKIEKKEFSQEGGEPEHESID 814
Query: 661 DETDSKPEGGESFDPINGVSSENLDDGGHSPSKQQEQQQQQKKKKPLLKKFGYLLKKKNS 720
DETDSKPEGGESFDPINGVSSENLDDGGHSPSKQQEQQQQQKKKKPLLKKFGYLLKKKNS
Sbjct: 815 DETDSKPEGGESFDPINGVSSENLDDGGHSPSKQQEQQQQQKKKKPLLKKFGYLLKKKNS 874
Query: 721 VNQKQ 726
VNQKQ
Sbjct: 875 VNQKQ 879
BLAST of Cucsa.318540 vs. TrEMBL
Match:
A0A061F0H8_THECC (Uncharacterized protein OS=Theobroma cacao GN=TCM_025519 PE=4 SV=1)
HSP 1 Score: 918.3 bits (2372), Expect = 5.8e-264
Identity = 498/724 (68.78%), Postives = 589/724 (81.35%), Query Frame = 1
Query: 1 MEQAGLEEAHKKEEEWEKEIEAVRSQHALDVAALLSTSQELQRVKMELAMTTDAKNQALS 60
+EQAG+E A KK+EEWEKEIE+VR+QHALDVAALLST+QELQRVK ELAMT DAKNQALS
Sbjct: 157 LEQAGIEAAQKKDEEWEKEIESVRNQHALDVAALLSTTQELQRVKQELAMTCDAKNQALS 216
Query: 61 HADDATKIAEIHVEKVEILSGELTRLKALLDSKLEMQSNENGQLIMKLKSEIDSLNLELE 120
HADDATKIAEIH EKVEILS EL RLK+LLDSK E ++NEN + +++LK+EI+SL ELE
Sbjct: 217 HADDATKIAEIHAEKVEILSAELVRLKSLLDSKRETEANENKE-VLRLKAEIESLKQELE 276
Query: 121 KAKSYAEMVKEKEVSIERLNSELKAAKMAETCYEETIMDKDASIEQLNIDLEAAKMAETY 180
KAK++ E + +M+K+A IEQLN+DLEAA+MAE+Y
Sbjct: 277 KAKTHEEKL---------------------------MMEKEAFIEQLNVDLEAARMAESY 336
Query: 181 AHGLVEEWKNRAEEMETKLDSANKLERSASESLDSVMKQLEHNNDLLHNAELEIAALKEK 240
AH +VEEWK+R EE+E +++ A KLERSASESLDSVMKQLE NN LH+AE EIAALKEK
Sbjct: 337 AHNVVEEWKSRVEELEMQIEEAKKLERSASESLDSVMKQLESNNYSLHDAESEIAALKEK 396
Query: 241 VGLLEMTVKRQKEDLKESEHHLHRAKEEASEMEKLVASLRAQLETVNEEKTQALNNEKLA 300
VGLLEMT+ RQ+ DL+ESEHH+ AKEE +E+ KLV SL++ LETV EEKTQALNNEKLA
Sbjct: 397 VGLLEMTIGRQRGDLEESEHHIKLAKEETAEVAKLVESLKSDLETVKEEKTQALNNEKLA 456
Query: 301 ASSVQSLLEEKNQLLNELETSKDEEEKSKKAMESLASALHEISTEARETKEKLLSSQADQ 360
ASSVQ+LLEEKN+L+NELE S+DEEEKSKKAMESLASALHE+S EARE KEKLLSS+ +
Sbjct: 457 ASSVQTLLEEKNKLINELENSRDEEEKSKKAMESLASALHEVSAEAREAKEKLLSSETEH 516
Query: 361 ENYESQIENLKLVLKATNEKYENMLENSNHEIDILTSTIEKSKHEYENSKAEWEEKELHL 420
ENYE+QIE+L+LVLKATNEKYE ML+++ + ID+LT+TIE+SK+EY+NSK EWE+KELHL
Sbjct: 517 ENYETQIEDLRLVLKATNEKYETMLDDAKNGIDLLTNTIEQSKNEYQNSKTEWEQKELHL 576
Query: 421 VDAVKKSEEENSSLDKEIDRLVNLLKQTEEEACKMREEEAQLKDSLKEVEAEVIYLQEAL 480
V+ VK+SEEENSSL+KEI+RLVNLLKQTEEEAC +EEEAQLK+SLKEVE+EVIYLQEAL
Sbjct: 577 VNCVKESEEENSSLEKEINRLVNLLKQTEEEACASKEEEAQLKESLKEVESEVIYLQEAL 636
Query: 481 GEAKSESMKLKESLLDKENEFQSIHQENEELLTREAASLKKVDELSKLLEEASAKKQTME 540
E K+ESMKLKESLLDKE E Q + QENEEL REAASLKK++ELSKLLEEA+ K+Q+ E
Sbjct: 637 KEVKTESMKLKESLLDKETELQGVIQENEELRAREAASLKKMEELSKLLEEATMKRQSEE 696
Query: 541 NGEPTDSEKDYDLLPKVVEFSEENGKRQEEKTKVEPPIPIEHEEHKFEFPWVGNGASDEK 600
NGE TDSEKDYDLLPKVVEFSEENG EEK K+E P + EE K E N S ++
Sbjct: 697 NGELTDSEKDYDLLPKVVEFSEENGHGSEEKPKLELPSE-QPEEPKKENSLEVNDVSKDE 756
Query: 601 TEKTDSAATLQNGNDKPKEAEKKEKEDDSVKVEYKMWESCKIEKKEFSQEGGEPEHESID 660
+TD A ++N N K KE E K KEDDSV+VE+KMWESCKIEKKEFS E EPE E +
Sbjct: 757 ALQTD-GAKVENVNGKLKEDESKGKEDDSVEVEFKMWESCKIEKKEFSPE-REPEQEFFE 816
Query: 661 DETDSKPEGGESFDPINGVSSENLDDGGHSPSKQQEQQQQQKKKKPLLKKFGYLLKKKNS 720
+E +SK G E FD ING+ +E++DDGG+SPSK QQQQKKKKPLL+KFG LLKKK S
Sbjct: 817 EEVESKVVGSEGFDQINGL-TESIDDGGNSPSK----QQQQKKKKPLLRKFGSLLKKKGS 844
Query: 721 VNQK 725
N K
Sbjct: 877 SNHK 844
BLAST of Cucsa.318540 vs. TrEMBL
Match:
B9I744_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0013s07650g PE=4 SV=2)
HSP 1 Score: 912.5 bits (2357), Expect = 3.2e-262
Identity = 493/726 (67.91%), Postives = 586/726 (80.72%), Query Frame = 1
Query: 1 MEQAGLEEAHKKEEEWEKEIEAVRSQHALDVAALLSTSQELQRVKMELAMTTDAKNQALS 60
+EQAG+E+A KKEEEW+KE+EAVRSQHALDV ALLST+QELQR+K ELAM TDAKNQALS
Sbjct: 160 LEQAGIEDAQKKEEEWQKELEAVRSQHALDVTALLSTTQELQRLKQELAMITDAKNQALS 219
Query: 61 HADDATKIAEIHVEKVEILSGELTRLKALLDSKLEMQSNENGQLIMKLKSEIDSLNLELE 120
HADDATKIAEIH EKVE+LS ELTRL LLDSKLE ++ E+ +++++L EIDSL +LE
Sbjct: 220 HADDATKIAEIHAEKVEMLSSELTRLNVLLDSKLETEAIESNKIVLQLNEEIDSLKQQLE 279
Query: 121 KAKSYAEMVKEKEVSIERLNSELKAAKMAETCYEETIMDKDASIEQLNIDLEAAKMAETY 180
K++ + E+ +++++A IEQLN++LEAAKMAE+Y
Sbjct: 280 KSEDF----------------------------EDKLIEREAFIEQLNVELEAAKMAESY 339
Query: 181 AHGLVEEWKNRAEEMETKLDSANKLERSASESLDSVMKQLEHNNDLLHNAELEIAALKEK 240
A LVEEWKNR EE+E + + ANKLERSASESL SVMKQLE NNDLLH+AE EIAALKEK
Sbjct: 340 ACNLVEEWKNRVEELEMQAEEANKLERSASESLGSVMKQLEANNDLLHDAETEIAALKEK 399
Query: 241 VGLLEMTVKRQKEDLKESEHHLHRAKEEASEMEKLVASLRAQLETVNEEKTQALNNEKLA 300
VGLLEMT++RQK DL+ESEH L KEEAS M K V SL ++LETV EEK QALNNEKLA
Sbjct: 400 VGLLEMTIRRQKGDLEESEHSLGMVKEEASVMVKKVESLMSELETVKEEKAQALNNEKLA 459
Query: 301 ASSVQSLLEEKNQLLNELETSKDEEEKSKKAMESLASALHEISTEARETKEKLLSSQADQ 360
ASSVQSLLEEKN+L+ ELE S+DEEEKSKKAMESLASALHE+S EARE KE+L+S+Q +
Sbjct: 460 ASSVQSLLEEKNKLITELENSRDEEEKSKKAMESLASALHEVSAEAREAKERLVSNQVEH 519
Query: 361 ENYESQIENLKLVLKATNEKYENMLENSNHEIDILTSTIEKSKHEYENSKAEWEEKELHL 420
ENYE+QIE+L+LVLKATNEKYE +L+++ HEID+L +T+E+SK++++NSKAEW++KE +L
Sbjct: 520 ENYETQIEDLRLVLKATNEKYETVLDDAKHEIDLLRNTVEESKNQFQNSKAEWDQKEKNL 579
Query: 421 VDAVKKSEEENSSLDKEIDRLVNLLKQTEEEACKMREEEAQLKDSLKEVEAEVIYLQEAL 480
+ ++KSEEENSSL+KEIDRLVNLL TEEEAC MR+EEA LKDSLKEVEAEVI LQEAL
Sbjct: 580 GNYLRKSEEENSSLEKEIDRLVNLLTHTEEEACGMRDEEAHLKDSLKEVEAEVISLQEAL 639
Query: 481 GEAKSESMKLKESLLDKENEFQSIHQENEELLTREAASLKKVDELSKLLEEASAKKQTME 540
GEA+ ESMKLKESLLDKENEFQ+I QENEEL T+EA+S KKV+ELSKLLEEA AKKQ E
Sbjct: 640 GEARVESMKLKESLLDKENEFQNIFQENEELRTKEASSHKKVEELSKLLEEAMAKKQVEE 699
Query: 541 NGEPTDSEKDYDLLPKVVEFSEENGKRQEEKTKVEPPIPIEHEEHKFEFPWVGNGASDEK 600
NGE TDSEKDYDLLPKVVEFSEENG +EEK +E P+ + +E + NGA+++
Sbjct: 700 NGELTDSEKDYDLLPKVVEFSEENGHVREEKPTMELPLQLSNELNTENAQEQINGATNKA 759
Query: 601 TEKTDSAATLQNGNDKPKEAEKKEKEDDSVKVEYKMWESCKIEKKEFSQEGGEPEHE-SI 660
+ A L+N N PKE E KEKEDDSV+VE+KMWESCKIEKKEFS E E EHE S
Sbjct: 760 AQM--DAHKLENVNGNPKEDESKEKEDDSVEVEFKMWESCKIEKKEFSPE-RETEHESSF 819
Query: 661 DDETDSKPEGGESFDPINGVSS-ENLDDGGHSPSKQQEQQQQQKKKKPLLKKFGYLLKKK 720
+DE DSK +GGESFD ING+SS EN+DDGG SPSK QQQQKKKKPLL+KF LLKKK
Sbjct: 820 EDEADSKVDGGESFDQINGLSSTENVDDGGSSPSK----QQQQKKKKPLLRKFSNLLKKK 850
Query: 721 NSVNQK 725
+ NQK
Sbjct: 880 GTSNQK 850
BLAST of Cucsa.318540 vs. TrEMBL
Match:
A0A067L4V1_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_02555 PE=4 SV=1)
HSP 1 Score: 904.4 bits (2336), Expect = 8.7e-260
Identity = 491/725 (67.72%), Postives = 585/725 (80.69%), Query Frame = 1
Query: 1 MEQAGLEEAHKKEEEWEKEIEAVRSQHALDVAALLSTSQELQRVKMELAMTTDAKNQALS 60
+EQAG+E A KKE+EW+KE+E+VR+QHALDVAALLS +QELQRVK ELAMTTDAKNQALS
Sbjct: 155 LEQAGIEAAKKKEDEWQKELESVRNQHALDVAALLSATQELQRVKQELAMTTDAKNQALS 214
Query: 61 HADDATKIAEIHVEKVEILSGELTRLKALLDSKLEMQSNENGQLIMKLKSEIDSLNLELE 120
HADDATKIAEIH +KVEILS ELTRLKALLDSK EM++NE+ +++M+LK EI++L EL+
Sbjct: 215 HADDATKIAEIHADKVEILSAELTRLKALLDSKHEMEANESNKIVMQLKEEIETLKQELK 274
Query: 121 KAKSYAEMVKEKEVSIERLNSELKAAKMAETCYEETIMDKDASIEQLNIDLEAAKMAETY 180
+AK E +++K+ASIEQLN++LEAAKMAE+Y
Sbjct: 275 RAKGV----------------------------ENELIEKEASIEQLNVELEAAKMAESY 334
Query: 181 AHGLVEEWKNRAEEMETKLDSANKLERSASESLDSVMKQLEHNNDLLHNAELEIAALKEK 240
A LV EWK R EE+E +++ ANKLERSASESL SVMKQLE NNDLLH+AE EI+ALKEK
Sbjct: 335 ARNLVAEWKCRIEELEMQVEEANKLERSASESLCSVMKQLEGNNDLLHDAESEISALKEK 394
Query: 241 VGLLEMTVKRQKEDLKESEHHLHRAKEEASEMEKLVASLRAQLETVNEEKTQALNNEKLA 300
+GLLEMT+ RQK DL+ESE L AKEE SEM K V SL+++L+ V EEK QALNNEKLA
Sbjct: 395 LGLLEMTITRQKGDLEESECCLSAAKEETSEMAKKVESLKSELDIVKEEKAQALNNEKLA 454
Query: 301 ASSVQSLLEEKNQLLNELETSKDEEEKSKKAMESLASALHEISTEARETKEKLLSSQADQ 360
ASSVQSLLEEKN+L+NELE S++EEEKSKKAMESLASALHE+S EARE KE+LLS+Q +
Sbjct: 455 ASSVQSLLEEKNKLINELENSREEEEKSKKAMESLASALHEVSAEAREAKEQLLSTQVEH 514
Query: 361 ENYESQIENLKLVLKATNEKYENMLENSNHEIDILTSTIEKSKHEYENSKAEWEEKELHL 420
ENYE+Q+ENL+LVLK NE+YE +L+++ HEI++L + IE SK+E++NSK EWE+KE +L
Sbjct: 515 ENYETQVENLRLVLKEANERYETILDDAKHEIEVLKNDIEDSKNEFQNSKDEWEQKEQNL 574
Query: 421 VDAVKKSEEENSSLDKEIDRLVNLLKQTEEEACKMREEEAQLKDSLKEVEAEVIYLQEAL 480
+D VK+SEE+NSSL++EIDRLVNLLK TEEEAC REEEA LKDSLKEVEAEVI LQE L
Sbjct: 575 IDCVKQSEEKNSSLEREIDRLVNLLKHTEEEACASREEEALLKDSLKEVEAEVISLQEGL 634
Query: 481 GEAKSESMKLKESLLDKENEFQSIHQENEELLTREAASLKKVDELSKLLEEASAKKQTME 540
GE++ ESMKLK+SLLDKENE Q++ QENEEL TREA SLKKV++LSKLLEEA AKKQT E
Sbjct: 635 GESRVESMKLKDSLLDKENELQNLIQENEELRTREAISLKKVEDLSKLLEEAIAKKQTEE 694
Query: 541 NGEPTDSEKDYDLLPKVVEFSEENGKRQEEKTKVEPPIPIEHEEHKFEFPWVGNGASDEK 600
NGE TDSEK+YDLLPKVVEFSEENG +EEK KVE P +HE+ E P N +
Sbjct: 695 NGELTDSEKEYDLLPKVVEFSEENGHVREEKPKVELP-QQQHEDVGKENPKEQNNGFNND 754
Query: 601 TEKTDSAATLQNGNDKPKEAEKKEKEDDSVKVEYKMWESCKIEKKEFSQEGGEPEHESID 660
T + AA ++NGN KPKE E KEKED+SV+ E+KMWESCKIEKKEFS E E EHES +
Sbjct: 755 TAPIE-AAKVENGNGKPKEDETKEKEDESVE-EFKMWESCKIEKKEFSPE-REHEHESFE 814
Query: 661 DETDSKPEGGESFDPINGVSS-ENLDDGGHSPSKQQEQQQQQKKKKPLLKKFGYLLKKKN 720
DE DSK EGGESFD +NG+SS EN+DDG SPSK QQQQKKKKPLL+KFG LLKKK+
Sbjct: 815 DEVDSKAEGGESFDQVNGLSSVENVDDGATSPSK----QQQQKKKKPLLRKFGSLLKKKS 843
Query: 721 SVNQK 725
+ NQK
Sbjct: 875 TSNQK 843
BLAST of Cucsa.318540 vs. TrEMBL
Match:
B9S250_RICCO (ATP binding protein, putative OS=Ricinus communis GN=RCOM_1327430 PE=4 SV=1)
HSP 1 Score: 901.7 bits (2329), Expect = 5.6e-259
Identity = 489/729 (67.08%), Postives = 584/729 (80.11%), Query Frame = 1
Query: 1 MEQAGLEEAHKKEEEWEKEIEAVRSQHALDVAALLSTSQELQRVKMELAMTTDAKNQALS 60
+EQAG+E A KKEEEW+KE+E+VR+QHA+DVA+LLST+QELQ+VK ELAMTTDAKNQAL+
Sbjct: 155 LEQAGIEAAQKKEEEWQKELESVRNQHAVDVASLLSTTQELQKVKQELAMTTDAKNQALN 214
Query: 61 HADDATKIAEIHVEKVEILSGELTRLKALLDSKLEMQSNENGQLIMKLKSEIDSLNLELE 120
HADDATKIAEIH +KVEILS EL RLKALLDSKLE ++NE+ +++ +L EID+L ELE
Sbjct: 215 HADDATKIAEIHADKVEILSSELIRLKALLDSKLETEANESHRMVAELTEEIDTLKQELE 274
Query: 121 KAKSYAEMVKEKEVSIERLNSELKAAKMAETCYEETIMDKDASIEQLNIDLEAAKMAETY 180
KA + E+ +++K+ASIEQLN++LEAAKMAE+Y
Sbjct: 275 KANGF----------------------------EDKLIEKEASIEQLNVELEAAKMAESY 334
Query: 181 AHGLVEEWKNRAEEMETKLDSANKLERSASESLDSVMKQLEHNNDLLHNAELEIAALKEK 240
A LV+EWK+R +E+E +++ AN+LERSASESL SVMKQLE NNDLLH+AE EIAALKEK
Sbjct: 335 ARSLVKEWKSRVDELEMQIEEANRLERSASESLCSVMKQLEGNNDLLHDAENEIAALKEK 394
Query: 241 VGLLEMTVKRQKEDLKESEHHLHRAKEEASEMEKLVASLRAQLETVNEEKTQALNNEKLA 300
VGLLEMT+ RQK DL+ESEH L AKEE +M K V SL+A+LE V EEK QALNNEKLA
Sbjct: 395 VGLLEMTIARQKGDLEESEHRLSVAKEETCDMVKKVQSLKAELEVVKEEKAQALNNEKLA 454
Query: 301 ASSVQSLLEEKNQLLNELETSKDEEEKSKKAMESLASALHEISTEARETKEKLLSSQADQ 360
ASSVQSLLEEKN+L+ ELE S++EEEKSKKAMESLASALHE+S EARE KEKL S+Q +
Sbjct: 455 ASSVQSLLEEKNKLITELENSREEEEKSKKAMESLASALHEVSAEAREAKEKLFSNQVEH 514
Query: 361 ENYESQIENLKLVLKATNEKYENMLENSNHEIDILTSTIEKSKHEYENSKAEWEEKELHL 420
E+YE+QIE+L+LVLK N++YE +++++ HEID+L +TIE+SK+E+ NSK EWE+KE +L
Sbjct: 515 ESYETQIEDLRLVLKEANQRYETVIDDTKHEIDLLKNTIEESKNEFLNSKTEWEQKEQNL 574
Query: 421 VDAVKKSEEENSSLDKEIDRLVNLLKQTEEEACKMREEEAQLKDSLKEVEAEVIYLQEAL 480
++ VKKS+EENSSL++EIDRLVNLLKQTEEEAC REEEAQLKDSLKEVEAEVI LQE L
Sbjct: 575 MNCVKKSDEENSSLEREIDRLVNLLKQTEEEACITREEEAQLKDSLKEVEAEVISLQETL 634
Query: 481 GEAKSESMKLKESLLDKENEFQSIHQENEELLTREAASLKKVDELSKLLEEASAKKQTME 540
GEAK ES+KLKESLLDKENE Q++ QENEEL TREA S KKV+ELSKLLEEA AKKQT E
Sbjct: 635 GEAKVESLKLKESLLDKENELQNLIQENEELRTREAVSQKKVEELSKLLEEAMAKKQTEE 694
Query: 541 NGEPTDSEKDYDLLPKVVEFSEENGKRQEEKTKVEPPIPIEHEEHKFEFPWVGNGASDEK 600
NGE TDSEKDYDLLPKVVEFSEENG EEK+K+E P+ +HE+ +GN
Sbjct: 695 NGELTDSEKDYDLLPKVVEFSEENGHVSEEKSKMEHPLH-QHED-------LGNSEEQNN 754
Query: 601 TEKTDS----AATLQNGNDKPKEAEKKEKEDDSVKVEYKMWESCKIEKKEFSQEGGEPEH 660
K DS A +N N KPK+ E KEKEDDSV+VE+KMWESCKIEKKEFS E E E
Sbjct: 755 GLKNDSIPTEGAKFENVNGKPKD-ESKEKEDDSVEVEFKMWESCKIEKKEFSPE-RETEQ 814
Query: 661 ESIDDETDSKPEGGESFDPINGVS-SENLDDGGHSPSKQQEQQQQQKKKKPLLKKFGYLL 720
ES +DE DSK EGGE FD ING+S +EN++DGG SPSK QQQQKKKKPLL+KFG LL
Sbjct: 815 ESFEDEGDSKAEGGEGFDQINGLSLTENVEDGGCSPSK----QQQQKKKKPLLRKFGSLL 841
Query: 721 KKKNSVNQK 725
KKK++ NQK
Sbjct: 875 KKKSTGNQK 841
BLAST of Cucsa.318540 vs. TAIR10
Match:
AT5G16730.1 (AT5G16730.1 Plant protein of unknown function (DUF827))
HSP 1 Score: 662.5 bits (1708), Expect = 2.9e-190
Identity = 379/728 (52.06%), Postives = 511/728 (70.19%), Query Frame = 1
Query: 3 QAGLEEAHKKEEEWEKEIEAVRSQHALDVAALLSTSQELQRVKMELAMTTDAKNQALSHA 62
+AG+E EEE +KE+E V++QHA D AAL++ QEL+++ ELA DAK++ALS A
Sbjct: 165 EAGIEAVQNNEEELKKELETVKNQHASDSAALVAVRQELEKINEELAAAFDAKSKALSQA 224
Query: 63 DDATKIAEIHVEKVEILSGELTRLKALLDSKLEMQSNENGQLIMKLKSEIDSLNLELEKA 122
+DA+K AEIH EKV+ILS ELTRLKALLDS E + + +++ KL+ EI L +LE A
Sbjct: 225 EDASKTAEIHAEKVDILSSELTRLKALLDSTREKTAISDNEMVAKLEDEIVVLKRDLESA 284
Query: 123 KSYAEMVKEKEVSIERLNSELKAAKMAETCYEETIMDKDASIEQLNIDLEAAKMAETYAH 182
+ + VKEKE+ +E+L N+DLEAAKMAE+ AH
Sbjct: 285 RGFEAEVKEKEMIVEKL----------------------------NVDLEAAKMAESNAH 344
Query: 183 GLVEEWKNRAEEMETKLDSANKLERSASESLDSVMKQLEHNNDLLHNAELEIAALKEKVG 242
L EW+++A+E+E +L+ ANKLERSAS SL+SVMKQLE +ND LH+ E EI LKE++
Sbjct: 345 SLSNEWQSKAKELEEQLEEANKLERSASVSLESVMKQLEGSNDKLHDTETEITDLKERIV 404
Query: 243 LLEMTVKRQKEDLKESEHHLHRAKEEASEMEKLVASLRAQLETVNEEKTQALNNEKLAAS 302
LE TV +QKEDL+ SE L +EE S+ EK V L+++LETV EEK +AL E+ A S
Sbjct: 405 TLETTVAKQKEDLEVSEQRLGSVEEEVSKNEKEVEKLKSELETVKEEKNRALKKEQDATS 464
Query: 303 SVQSLLEEKNQLLNELETSKDEEEKSKKAMESLASALHEISTEARETKEKLLSSQADQEN 362
VQ L EEK++LL++LE+SK+EEEKSKKAMESLASALHE+S+E RE KEKLL SQ D E
Sbjct: 465 RVQRLSEEKSKLLSDLESSKEEEEKSKKAMESLASALHEVSSEGRELKEKLL-SQGDHE- 524
Query: 363 YESQIENLKLVLKATNEKYENMLENSNHEIDILTSTIEKSKHEYENSKAEWEEKELHLVD 422
YE+QI++LKLV+KATNEKYENML+ + HEID+L S +E++K +E+SK +WE KE +LV+
Sbjct: 525 YETQIDDLKLVIKATNEKYENMLDEARHEIDVLVSAVEQTKKHFESSKKDWEMKEANLVN 584
Query: 423 AVKKSEEENSSLDKEIDRLVNLLKQTEEEACKMREEEAQLKDSLKEVEAEVIYLQEALGE 482
VKK EE+ +S+ KE++RL NLLK+TEEEA ++EAQ KDSLKEVE E++YLQE LGE
Sbjct: 585 YVKKMEEDVASMGKEMNRLDNLLKRTEEEADAAWKKEAQTKDSLKEVEEEIVYLQETLGE 644
Query: 483 AKSESMKLKESLLDKENEFQSIHQENEELLTREAASLKKVDELSKLLEEA-SAKKQ-TME 542
AK+ESMKLKE+LLDKE EFQ++ ENE+L +E SLKK++ELSKLLEEA AKKQ E
Sbjct: 645 AKAESMKLKENLLDKETEFQNVIHENEDLKAKEDVSLKKIEELSKLLEEAILAKKQPEEE 704
Query: 543 NGEPTDSEKDYDLLPKVVEFSEENGKR--QEEKTKVEPPIPIEHEEHKFEFPWVGNGASD 602
NGE ++SEKDYDLLPKVVEFS ENG R +E+ KVE ++HE + + + +
Sbjct: 705 NGELSESEKDYDLLPKVVEFSSENGHRSVEEKSAKVE---TLDHEPPQEQISNGNSNGNG 764
Query: 603 EKTEKTDSAATLQNGNDKPKEAEKKEKEDDSVKVEYKMWESCKIEKKEFSQEGGEPEHES 662
+ ++ + ++ + K+ + + +DDSV+V +KMWESC+IEKKE + + E ES
Sbjct: 765 MEEKEVNGKPEVETEKKEKKDESQDDDKDDSVEVIFKMWESCQIEKKEAFPD-KKSELES 824
Query: 663 IDDETDSKPEGGESFDPINGVSSENLDDGGHSPSKQQE--QQQQQKKKKPLLKKFGYLLK 722
++E DS D + S+EN+D+ G++ + + + +++ KKKK LL K G LLK
Sbjct: 825 QEEEEDS-----SKIDESDKTSTENIDETGNALTAEDQLTMEKKIKKKKTLLGKVGNLLK 853
Query: 723 KKNSVNQK 725
KK VNQK
Sbjct: 885 KKAPVNQK 853
BLAST of Cucsa.318540 vs. TAIR10
Match:
AT3G02930.1 (AT3G02930.1 Plant protein of unknown function (DUF827))
HSP 1 Score: 644.0 bits (1660), Expect = 1.1e-184
Identity = 376/725 (51.86%), Postives = 496/725 (68.41%), Query Frame = 1
Query: 3 QAGLEEAHKKEEEWEKEIEAVRSQHALDVAALLSTSQELQRVKMELAMTTDAKNQALSHA 62
+AG+E +KEEE +KE+E V++QHA + A LL +QEL+ V ELA DAK++AL A
Sbjct: 154 EAGIEAVQRKEEELKKELENVKNQHASESATLLLVTQELENVNQELANAKDAKSKALCRA 213
Query: 63 DDATKIAEIHVEKVEILSGELTRLKALLDSKLEMQSNENGQLIMKLKSEIDSLNLELEKA 122
DDA+K+A IH EKVEILS EL RLKALLDS E + ++ +KL +EI L +LE A
Sbjct: 214 DDASKMAAIHAEKVEILSSELIRLKALLDSTREKEIISKNEIALKLGAEIVDLKRDLENA 273
Query: 123 KSYAEMVKEKEVSIERLNSELKAAKMAETCYEETIMDKDASIEQLNIDLEAAKMAETYAH 182
+S VKE E+ IE QLN+DLEAAKMAE+YAH
Sbjct: 274 RSLEAKVKELEMIIE----------------------------QLNVDLEAAKMAESYAH 333
Query: 183 GLVEEWKNRAEEMETKLDSANKLERSASESLDSVMKQLEHNNDLLHNAELEIAALKEKVG 242
G +EW+N+A+E+E +L+ ANKLE+ AS SL SV KQLE +N LH+ E EI LKEK+
Sbjct: 334 GFADEWQNKAKELEKRLEEANKLEKCASVSLVSVTKQLEVSNSRLHDMESEITDLKEKIE 393
Query: 243 LLEMTVKRQKEDLKESEHHLHRAKEEASEMEKLVASLRAQLETVNEEKTQALNNEKLAAS 302
LLEMTV QK DL++SE L A+EE+S+ EK L+ +LETVNEEKTQAL E+ A S
Sbjct: 394 LLEMTVASQKVDLEKSEQKLGIAEEESSKSEKEAEKLKNELETVNEEKTQALKKEQDATS 453
Query: 303 SVQSLLEEKNQLLNELETSKDEEEKSKKAMESLASALHEISTEARETKEKLLSSQADQEN 362
SVQ LLEEK ++L+ELE+SK+EEEKSKKAMESLASALHE+S+E+RE KEKLLS + DQ N
Sbjct: 454 SVQRLLEEKKKILSELESSKEEEEKSKKAMESLASALHEVSSESRELKEKLLS-RGDQ-N 513
Query: 363 YESQIENLKLVLKATNEKYENMLENSNHEIDILTSTIEKSKHEYENSKAEWEEKELHLVD 422
YE+QIE+LKLV+KATN KYENML+ + HEID+L + +E++K ++E++ +WE +E LV+
Sbjct: 514 YETQIEDLKLVIKATNNKYENMLDEARHEIDVLVNAVEQTKKQFESAMVDWEMREAGLVN 573
Query: 423 AVKKSEEENSSLDKEIDRLVNLLKQTEEEACKMREEEAQLKDSLKEVEAEVIYLQEALGE 482
VK+ +EE SS+ KE++RL NL+K+T+EEA E+E+Q++D LKEVE EVIYLQE L E
Sbjct: 574 HVKEFDEEVSSMGKEMNRLGNLVKRTKEEADASWEKESQMRDCLKEVEDEVIYLQETLRE 633
Query: 483 AKSESMKLKESLLDKENEFQSIHQENEELLTREAASLKKVDELSKLLEEASAKKQTMENG 542
AK+E++KLK +LDKE EFQSI EN+EL ++ SLKK+ ELS+LLEEA AKK ENG
Sbjct: 634 AKAETLKLKGKMLDKETEFQSIVHENDELRVKQDDSLKKIKELSELLEEALAKKHIEENG 693
Query: 543 EPTDSEKDYDLLPKVVEFSEENGKR--QEEKTKVEPPIPIEHEEHKFEFPWVGNGASDEK 602
E ++SEKDYDLLPKVVEFSEENG R +E+ +KVE ++ K E E
Sbjct: 694 ELSESEKDYDLLPKVVEFSEENGYRSAEEKSSKVE---TLDGMNMKLE----------ED 753
Query: 603 TEKTDSAATLQNGNDKPKEAEKKEKEDDSVKVEYKMWESCKIEKKE-FSQEGGEPEHESI 662
TEK KE +++ ED++V+VE+KMWESC+IEKKE F +E + E E +
Sbjct: 754 TEK--------------KEKKERSPEDETVEVEFKMWESCQIEKKEVFHKESAKEEEEDL 806
Query: 663 DDETDSKPEGGESFDPINGVSSENLDDGGHSPSKQQEQQQQQKKKKPLLKKFGYLLKKKN 722
+ S + P+NG++ E+ + +++++KKKK L K G LLKKK
Sbjct: 814 NVVDQS-----QKTSPVNGLTGED----------ELLKEKEKKKKKTLFGKVGNLLKKKG 806
Query: 723 SVNQK 725
VNQK
Sbjct: 874 PVNQK 806
HSP 2 Score: 36.6 bits (83), Expect = 7.9e-02
Identity = 35/137 (25.55%), Postives = 65/137 (47.45%), Query Frame = 1
Query: 411 AEWEEKELHLVDAVKKSEEENSSLDKE----IDRLVNLLKQTEEEACKMREEEAQLKDSL 470
+E + + + + + +KK+ E +SL+ E +D+L K+ EE + K+ E K SL
Sbjct: 83 SESQPQSVQIKEDLKKANELIASLENEKAKALDQLKEARKEAEEASEKLDEALEAQKKSL 142
Query: 471 KEVEAEVIYLQEALGEA---KSESMKLK------------ESLLDKENEFQSIHQENEEL 529
+ E E + EA EA K E +K + +LL E ++++QE
Sbjct: 143 ENFEIEKFEVVEAGIEAVQRKEEELKKELENVKNQHASESATLLLVTQELENVNQELANA 202
BLAST of Cucsa.318540 vs. TAIR10
Match:
AT1G65010.1 (AT1G65010.1 Plant protein of unknown function (DUF827))
HSP 1 Score: 525.8 bits (1353), Expect = 4.3e-149
Identity = 317/726 (43.66%), Postives = 453/726 (62.40%), Query Frame = 1
Query: 1 MEQAGLEEAHKKEEEWEKEIEAVRSQHALDVAALLSTSQELQRVKMELAMTTDAKNQALS 60
+EQAGLE KK+ + E+E++RSQHALD++ALLST++ELQRVK EL+MT DAKN+ALS
Sbjct: 153 LEQAGLEAVQKKDVTSKNELESIRSQHALDISALLSTTEELQRVKHELSMTADAKNKALS 212
Query: 61 HADDATKIAEIHVEKVEILSGELTRLKALLDSKLEMQSNENGQLIMKLKSEIDSLNLELE 120
HA++ATKIAEIH EK EIL+ EL RLKALL SK E ++ E +++ KLKSEI+ L ELE
Sbjct: 213 HAEEATKIAEIHAEKAEILASELGRLKALLGSKEEKEAIEGNEIVSKLKSEIELLRGELE 272
Query: 121 KAKSYAEMVKEKEVSIERLNSELKAAKMAETCYEETIMDKDASIEQLNIDLEAAKMAETY 180
K +KE+E +EQL +DLEAAKMAE+
Sbjct: 273 KVSILESSLKEQE----------------------------GLVEQLKVDLEAAKMAESC 332
Query: 181 AHGLVEEWKNRAEEMETKLDSANKLERSASESLDSVMKQLEHNNDLLHNAELEIAALKEK 240
+ VEEWKN+ E+E +++ +N+ + SASES++SVMKQL N +LH + + AA KEK
Sbjct: 333 TNSSVEEWKNKVHELEKEVEESNRSKSSASESMESVMKQLAELNHVLHETKSDNAAQKEK 392
Query: 241 VGLLEMTVKRQKEDLKESEHHLHRAKEEASEMEKLVASLRAQLETVNEEKTQALNNEKLA 300
+ LLE T++ Q+ DL+E + AKEEAS++E LV S++++LE EEKT+AL+NEK A
Sbjct: 393 IELLEKTIEAQRTDLEEYGRQVCIAKEEASKLENLVESIKSELEISQEEKTRALDNEKAA 452
Query: 301 ASSVQSLLEEKNQLLNELETSKDEEEKSKKAMESLASALHEISTEARETKEKLLSSQADQ 360
S++Q+LL+++ +L ELE K EEEKSKK MESL AL E STE+ E K LL Q +
Sbjct: 453 TSNIQNLLDQRTELSIELERCKVEEEKSKKDMESLTLALQEASTESSEAKATLLVCQEEL 512
Query: 361 ENYESQIENLKLVLKATNEKYENMLENSNHEIDILTSTIEKSKHEYENSKAEWEEKELHL 420
+N ESQ+++LKL K TNEKYE MLE++ +EID L ST++ ++E+ENSKA WE+KELHL
Sbjct: 513 KNCESQVDSLKLASKETNEKYEKMLEDARNEIDSLKSTVDSIQNEFENSKAGWEQKELHL 572
Query: 421 VDAVKKSEEENSSLDKEIDRLVNLLKQTEEEACKMREEEAQLKDSLKEVEAEVIYLQEAL 480
+ VKKSEEENSS +E+ RLVNLLK++EE+AC +EEEA LK++LK E EV YLQE L
Sbjct: 573 MGCVKKSEEENSSSQEEVSRLVNLLKESEEDACARKEEEASLKNNLKVAEGEVKYLQETL 632
Query: 481 GEAKSESMKLKESLLDKENEFQSIHQENEELLTREAASLKKVDELSKLLEEASAKKQTME 540
GEAK+ESMKLKESLLDKE + +++ E L E + L+K++ELSK+ E K+ ++
Sbjct: 633 GEAKAESMKLKESLLDKEEDLKNVTAEISSLREWEGSVLEKIEELSKVKESLVDKETKLQ 692
Query: 541 NGEPTDSE---KDYDLLPKVVEFSEENGKRQEEKTKVEPPIPIEHEEHKFEFPWVG--NG 600
+ E ++ + ++ E S N +E TK++ + + + E ++
Sbjct: 693 SITQEAEELKGREAAHMKQIEELSTANASLVDEATKLQSIVQESEDLKEKEAGYLKKIEE 752
Query: 601 ASDEKTEKTDSAATLQNGNDKPKEAEKKEKEDDSVKVEYKMWESCKIEKKEFSQEG---G 660
S D+ LQ+ + K+ +++E V Y KIE+ + E
Sbjct: 753 LSVANESLADNVTDLQSIVQESKDLKERE-------VAYLK----KIEELSVANESLVDK 812
Query: 661 EPEHESIDDETDS-KPEGGESFDPINGVSSEN---LDDGGHSPSKQQEQQQQQKKKKPLL 715
E + + ID E + + I +S EN +D+ + + +E + ++++ L
Sbjct: 813 ETKLQHIDQEAEELRGREASHLKKIEELSKENENLVDNVANMQNIAEESKDLREREVAYL 839
HSP 2 Score: 171.8 bits (434), Expect = 1.6e-42
Identity = 201/798 (25.19%), Postives = 356/798 (44.61%), Query Frame = 1
Query: 2 EQAGLEEAHKKEEEWEKEIEAVRSQHALDVAALLSTSQELQRVKMELAMTTDAKNQALSH 61
E L+E+ +EE K + A S ++L +EL +VK L + + K Q+++
Sbjct: 610 ESMKLKESLLDKEEDLKNVTAEISSLREWEGSVLEKIEELSKVKESL-VDKETKLQSITQ 669
Query: 62 ADDATKIAEI-HVEKVEILS-------GELTRLKALLDSKLEMQSNENGQL--IMKLKSE 121
+ K E H++++E LS E T+L++++ +++ E G L I +L
Sbjct: 670 EAEELKGREAAHMKQIEELSTANASLVDEATKLQSIVQESEDLKEKEAGYLKKIEELSVA 729
Query: 122 IDSLN---LELEKAKSYAEMVKEKEVSIERLNSELKAAKMAETCYEETIMDKDASIEQLN 181
+SL +L+ ++ +KE+EV+ + EL A E+++DK+ ++ ++
Sbjct: 730 NESLADNVTDLQSIVQESKDLKEREVAYLKKIEELSVAN-------ESLVDKETKLQHID 789
Query: 182 IDLEAAKMAETYAHGLVEEWKNRAEEMETKLDSANKLERSASESLDSVMKQLEHNNDLLH 241
+ E + E +EE ++E E +D+ ++ A ES D +++ + +
Sbjct: 790 QEAEELRGREASHLKKIEEL---SKENENLVDNVANMQNIAEESKDLREREVAYLKKI-- 849
Query: 242 NAELEIA--ALKEKVGLLEMTVKRQKEDLKESEHHLHRAKEEASEMEKLVASLRAQLETV 301
EL A L + V L+ + + ++L+E E L + EE SE+ + + ++L+TV
Sbjct: 850 -DELSTANGTLADNVTNLQ-NISEENKELRERETTLLKKAEELSELNESLVDKASKLQTV 909
Query: 302 NEEKTQALNNEKLAASSVQSLLEEKNQLLNELETSKDEEEKSKKAMESLASALHEISTEA 361
+E + E ++ L + +++L++ ET K+ ++ +A + E
Sbjct: 910 VQENEELRERETAYLKKIEEL-SKLHEILSDQETKLQISNHEKEELKERETAYLKKIEEL 969
Query: 362 RETKEKLLSSQADQENYESQIENLKLVLKATNEKYENMLENSNHEIDILTSTIEKSKHEY 421
+ +E LL+ + + +IE+L+ +K E E
Sbjct: 970 SKVQEDLLNKENELHGMVVEIEDLRSKDSLAQKKIE----------------------EL 1029
Query: 422 ENSKAEWEEKELHLVDAVKKSEEENSSLDKEIDRLVNLLKQTEEEACKMREEEAQLKDSL 481
N A KE L V ++EE LK + K +E + LK SL
Sbjct: 1030 SNFNASLLIKENELQAVVCENEE---------------LKSKQVSTLKTIDELSDLKQSL 1089
Query: 482 ----KEVEAEVIYLQEALGEAK------SESMKLKESLLDKENEFQSIHQENEELLTREA 541
KE++A ++ ++ EA E LK++L+DK+NE Q + ENEEL +EA
Sbjct: 1090 IHKEKELQAAIVENEKLKAEAALSLQRIEELTNLKQTLIDKQNELQGVFHENEELKAKEA 1149
Query: 542 ASLKKVDEL-----SKLLEEASAKKQTMENGEPTDSEKDYDLLPKVVEFS--EENGKRQE 601
+SLKK+DEL S L +E+ ++ T EN E +D K+ E S +E+ +E
Sbjct: 1150 SSLKKIDELLHLEQSWLEKESEFQRVTQENLEL--KTQDALAAKKIEELSKLKESLLEKE 1209
Query: 602 EKTKVEPPIPIEHEEHKFEFPWVGNGASDEKTEKTDSAATLQ-----NGNDKPKEAEKKE 661
+ K +E E + GN + + D + NG++K K ++
Sbjct: 1210 TELKCREAAALEKMEEPSKH---GNSELNSIGKDYDLVQFSEVNGASNGDEKTKTDHYQQ 1269
Query: 662 KEDDSV-----------------------------------KVEYKMWESCKIEKKEFSQ 721
+ + + + E+KMW+S KIEK E S
Sbjct: 1270 RSREHMIQESPMEAIDKHLMGERAAIHKVAHRVEGERNVEKESEFKMWDSYKIEKSEVSP 1329
Query: 722 EGGEPEHESIDDETDSKPEGGESFDPI-NGVS-SENLDDGGHSPSKQQEQQQQQKKKKPL 726
E E E +S+++E DSK E E+ D NG S +++ +D G+ K +Q KKKKPL
Sbjct: 1330 E-RETELDSVEEEVDSKAESSENMDQYSNGFSLTDHTEDSGNLLLK----EQHMKKKKPL 1344
HSP 3 Score: 109.8 bits (273), Expect = 7.3e-24
Identity = 160/672 (23.81%), Postives = 288/672 (42.86%), Query Frame = 1
Query: 11 KKEEEWEKEIEAVRS---QHALDVAALLSTSQELQR----VKMELAMTTDAKNQALSHAD 70
+K E EK IEA R+ ++ V + +L+ +K EL ++ + K +AL +
Sbjct: 363 EKIELLEKTIEAQRTDLEEYGRQVCIAKEEASKLENLVESIKSELEISQEEKTRALDNEK 422
Query: 71 DATKIAEIHVEKVEILSGELTRLKALLD-SKLEMQSNENGQLIMKLKSEIDSLNLELEKA 130
AT + +++ LS EL R K + SK +M+S L + + E +A
Sbjct: 423 AATSNIQNLLDQRTELSIELERCKVEEEKSKKDMES---------LTLALQEASTESSEA 482
Query: 131 KSYAEMVKEKEVSIERLNSELK-AAKMAETCYEETIMDKDASIEQLNIDLEAAK------ 190
K+ + +E+ + E LK A+K YE+ + D I+ L +++ +
Sbjct: 483 KATLLVCQEELKNCESQVDSLKLASKETNEKYEKMLEDARNEIDSLKSTVDSIQNEFENS 542
Query: 191 -----MAETYAHGLVEEWKNRAEEMETKLDSANKLERSASESLDSVMKQLEHNNDLLHN- 250
E + G V K EE + + ++L ES + + E L +N
Sbjct: 543 KAGWEQKELHLMGCV---KKSEEENSSSQEEVSRLVNLLKESEEDACARKEEEASLKNNL 602
Query: 251 --AELEIAALKEKVGLLEMTVKRQKEDLKESEHHLHRAKEEASEMEKLVASLRAQLETVN 310
AE E+ L+E +G + + KE L + E L E S + + S+ ++E ++
Sbjct: 603 KVAEGEVKYLQETLGEAKAESMKLKESLLDKEEDLKNVTAEISSLREWEGSVLEKIEELS 662
Query: 311 EEKTQALNNEKLAASSVQSLLEEKNQLLNELETSKDEEEKSKKAMESL---ASALHEIST 370
+ K ++ E + +QS+ +E +L + E+ A SL A+ L I
Sbjct: 663 KVKESLVDKE----TKLQSITQEAEELKGREAAHMKQIEELSTANASLVDEATKLQSIVQ 722
Query: 371 EARETKEKLLSSQADQENYESQIENLKLVLKATNEKYENMLENSNHEIDILTSTIEKSKH 430
E+ + KEK + Y +IE L + E++ +N + L S +++SK
Sbjct: 723 ESEDLKEK-------EAGYLKKIEELSVA-------NESLADN----VTDLQSIVQESK- 782
Query: 431 EYENSKAEWEEKELHLVDAVKKSEEENSSL-DKEIDRLVNLLKQTEEEACKMREEEAQLK 490
+ +E+E+ + +++ N SL DKE L+ ++EA ++R EA
Sbjct: 783 -------DLKEREVAYLKKIEELSVANESLVDKETK-----LQHIDQEAEELRGREAS-- 842
Query: 491 DSLKEVEAEVIYLQEALGEAKSESMKLKESLLDKENEFQSIHQENEELLTREAASLKKVD 550
LK++E E K E+L+D Q+I +E+++L RE A LKK+D
Sbjct: 843 -HLKKIE---------------ELSKENENLVDNVANMQNIAEESKDLREREVAYLKKID 902
Query: 551 ELSK---LLEEASAKKQTMENGEPTDSEKDYDLLPKVVEFSEENGKRQEEKTKVEPPIPI 610
ELS L + Q + E++ LL K E SE N ++ +K++ +
Sbjct: 903 ELSTANGTLADNVTNLQNISEENKELRERETTLLKKAEELSELNESLVDKASKLQTVVQE 962
Query: 611 EHEEHKFEFPWVGNGASDEKTEK--TDSAATLQNGNDKPKEAEKKEKEDDSVKVEYKMWE 651
E + E ++ K + +D LQ N + +E KE+E +K K+ E
Sbjct: 963 NEELRERETAYLKKIEELSKLHEILSDQETKLQISNHEKEEL--KERETAYLK---KIEE 964
HSP 4 Score: 106.7 bits (265), Expect = 6.2e-23
Identity = 162/732 (22.13%), Postives = 300/732 (40.98%), Query Frame = 1
Query: 4 AGLEEAHKKEEEWEKEIEAVRSQHALDVAALLSTSQELQRVKMELAMTTDAKNQALSHAD 63
+ +EE K E EKE+E + S S+ ++ V +LA ++ S D
Sbjct: 307 SSVEEWKNKVHELEKEVEESNRSKS-------SASESMESVMKQLAELNHVLHETKS--D 366
Query: 64 DATKIAEIHVEKVEILSGELTRLKALLDSKLEMQSNENGQLIMKLKSEIDSLNLELEKAK 123
+A + EK+E+L + + L+ E G+ + K E L +E K
Sbjct: 367 NAAQ-----KEKIELLEKTIEAQRTDLE--------EYGRQVCIAKEEASKLENLVESIK 426
Query: 124 SYAEMVKEKEVSIERLNSELKAAKMAETCYEETIMDKDASIEQLNIDLEAAKM-AETYAH 183
S E+ +E++ L++E A + ++ + +E+ ++ E +K E+
Sbjct: 427 SELEISQEEKT--RALDNEKAATSNIQNLLDQRT-ELSIELERCKVEEEKSKKDMESLTL 486
Query: 184 GLVEEWKNRAEEMETKLDSANKLERSASESLDSVMKQLEHNND----LLHNAELEIAALK 243
L E +E T L +L+ S+ +DS+ + N+ +L +A EI +LK
Sbjct: 487 ALQEASTESSEAKATLLVCQEELKNCESQ-VDSLKLASKETNEKYEKMLEDARNEIDSLK 546
Query: 244 EKVGLLEMTVKRQKEDLKESEHHLHRAKEEASEMEKLVASLRAQLETVNEEKTQALNNEK 303
V ++ + K ++ E HL +++ E + + EE ++ +N K
Sbjct: 547 STVDSIQNEFENSKAGWEQKELHLMGCVKKSEE----------ENSSSQEEVSRLVNLLK 606
Query: 304 LAASSVQSLLEEKNQLLNELETSKDEEEKSKKAMESLASALHEISTEARETKEKLLSSQA 363
+ + EE+ L N L+ ++ E ++ L L E E+ + KE LL +
Sbjct: 607 ESEEDACARKEEEASLKNNLKVAEGE-------VKYLQETLGEAKAESMKLKESLLDKEE 666
Query: 364 DQENYESQIENLKLVLKATNEKYENMLENSNHEIDILTSTIEKSKHEYENSKAEWEEKEL 423
D +N ++I +L+ + EK E + + +D T ++ E E K +E
Sbjct: 667 DLKNVTAEISSLREWEGSVLEKIEELSKVKESLVDKETK-LQSITQEAEELKG----REA 726
Query: 424 HLVDAVKKSEEENSSLDKEIDRLVNLLKQTEEEACKMREEEAQLKDSLKEVEAEVIYLQE 483
+ +++ N+SL E +L ++++++E+ LKE EA + E
Sbjct: 727 AHMKQIEELSTANASLVDEATKLQSIVQESED---------------LKEKEAGYLKKIE 786
Query: 484 ALGEAKSESMKLKESLLDKENEFQSIHQENEELLTREAASLKKVDELSKLLEEASAKKQT 543
L A ESL D + QSI QE+++L RE A LKK++ELS E K+
Sbjct: 787 ELSVAN-------ESLADNVTDLQSIVQESKDLKEREVAYLKKIEELSVANESLVDKETK 846
Query: 544 MENGEPTDSE---KDYDLLPKVVEFSEENGKRQEEKTKVEPPIPIEHEEHKFEFPWVG-- 603
+++ + E ++ L K+ E S+EN + ++ + + E ++
Sbjct: 847 LQHIDQEAEELRGREASHLKKIEELSKENENLVDNVANMQNIAEESKDLREREVAYLKKI 906
Query: 604 NGASDEKTEKTDSAATLQNGNDKPKEAEKKEKEDDSVKVEYKMWESCKIEKKEFSQEGGE 663
+ S D+ LQN +++ KE ++E E ++K Q +
Sbjct: 907 DELSTANGTLADNVTNLQNISEENKELRERETTLLKKAEELSELNESLVDKASKLQTVVQ 966
Query: 664 PEHESIDDETD--SKPEGGESFDPINGVSSENLDDGGHSPSKQQEQQQQQKKK-KPLLKK 723
E + ET K E I L H + +E++ KK + L K
Sbjct: 967 ENEELRERETAYLKKIEELSKLHEILSDQETKLQISNHEKEELKERETAYLKKIEELSKV 968
HSP 5 Score: 102.4 bits (254), Expect = 1.2e-21
Identity = 143/657 (21.77%), Postives = 281/657 (42.77%), Query Frame = 1
Query: 6 LEEAHKKEEEWEKEIEAVR-------SQHALDVAALLSTSQELQRVKMEL---------- 65
LE +EE+ +K++E++ ++ + A LL +EL+ + ++
Sbjct: 442 LERCKVEEEKSKKDMESLTLALQEASTESSEAKATLLVCQEELKNCESQVDSLKLASKET 501
Query: 66 -----AMTTDAKNQALSHADDATKIA-EIHVEKVEILSGELTRLKALLDSKLEMQSNEN- 125
M DA+N+ S I E K EL + + S+ E S++
Sbjct: 502 NEKYEKMLEDARNEIDSLKSTVDSIQNEFENSKAGWEQKELHLMGCVKKSEEENSSSQEE 561
Query: 126 -GQLIMKLKSEIDSLNLELEKAKSYAEMVKEKEVSIERLNSELKAAKMAETCYEETIMDK 185
+L+ LK + E+ S +K E ++ L L AK +E+++DK
Sbjct: 562 VSRLVNLLKESEEDACARKEEEASLKNNLKVAEGEVKYLQETLGEAKAESMKLKESLLDK 621
Query: 186 DASIEQLNIDLEAAKMAETYAHGLVEEWKNRAEEMETKLDSANKLERSASESLDSVMKQL 245
+ ++ N+ E + + E + ++E+ + ++ E+ +D KL+ E+ + ++
Sbjct: 622 EEDLK--NVTAEISSLRE-WEGSVLEKIEELSKVKESLVDKETKLQSITQEAEELKGREA 681
Query: 246 EHNNDLLHNAELEIAALKEKVGLLEMTVKRQKEDLKESEHHLHRAKEEASEMEKLVASLR 305
H + + + + E L ++ ++ EDLKE E + EE S + +A
Sbjct: 682 AHMKQIEELSTANASLVDEATKL--QSIVQESEDLKEKEAGYLKKIEELSVANESLADNV 741
Query: 306 AQLETVNEEKTQALNNEKLAASSVQSLLEEKNQLLNELETSKDEEEKSKKAMESLASALH 365
L+++ +E E ++ L L+++ + ++++++ AS L
Sbjct: 742 TDLQSIVQESKDLKEREVAYLKKIEELSVANESLVDKETKLQHIDQEAEELRGREASHLK 801
Query: 366 EISTEARETKEKLLSSQADQENYESQIENLKLVLKATNEKYENMLENSNHEIDILTSTIE 425
+I ++E E L+ + A+ +N + ++L+ A +K + + + D +T+
Sbjct: 802 KIEELSKEN-ENLVDNVANMQNIAEESKDLREREVAYLKKIDELSTANGTLADNVTNLQN 861
Query: 426 KSKHEYENSKAEWEEKELHLVDAVKKSEEENSSLDKEIDRLVNLLKQTEEEACKMREEEA 485
S+ EN E E+E L+ ++ E N SL + +L ++++ EE +RE E
Sbjct: 862 ISE---ENK--ELRERETTLLKKAEELSELNESLVDKASKLQTVVQENEE----LRERET 921
Query: 486 QLKDSLKEVEAEVIYLQEALGEAKSESMKLKESLLDKENEFQSIHQENEELLTREAASLK 545
LK++E E KL E L D+E + Q + E EEL RE A LK
Sbjct: 922 AY---LKKIE---------------ELSKLHEILSDQETKLQISNHEKEELKERETAYLK 981
Query: 546 KVDELSKLLEEASAKKQTMENGEPTDSE----KDYDLLPKVVEFSEENGKRQEEKTKVEP 605
K++ELSK+ E+ K+ + +G + E KD K+ E S N ++ +++
Sbjct: 982 KIEELSKVQEDLLNKENEL-HGMVVEIEDLRSKDSLAQKKIEELSNFNASLLIKENELQ- 1041
Query: 606 PIPIEHEEHKFEFPWVGNGASDEKTEKTDSAATLQNGNDKPKEAEKKEKEDDSVKVE 634
+ E+EE K+++ + T+ +D + KEKE + VE
Sbjct: 1042 AVVCENEE--------------LKSKQVSTLKTIDELSDLKQSLIHKEKELQAAIVE 1049
HSP 6 Score: 77.8 bits (190), Expect = 3.1e-14
Identity = 105/461 (22.78%), Postives = 196/461 (42.52%), Query Frame = 1
Query: 227 LHNAELEIAALKEKVGLLEMTVKRQKEDLKESEHHLHRAKEEASEMEKLVASLRAQLETV 286
L+ + ++ E++ LL+ + +DLKESE + A E+ E L A RA+ E+
Sbjct: 87 LNQIQEDLKKADEQIELLKKDKAKAIDDLKESEKLVEEANEKLK--EALAAQKRAE-ESF 146
Query: 287 NEEKTQALNNEKLAASSVQSL-LEEKNQL----------LNELETSKDEEEKSKKAMESL 346
EK +A+ E+ +VQ + KN+L ++ L ++ +E ++ K +
Sbjct: 147 EVEKFRAVELEQAGLEAVQKKDVTSKNELESIRSQHALDISALLSTTEELQRVKHELSMT 206
Query: 347 ASALHEISTEARETKEKLLSSQADQENYESQIENLKLVLKATNEKY----ENMLENSNHE 406
A A ++ + A E + E S++ LK +L + EK ++ E
Sbjct: 207 ADAKNKALSHAEEATKIAEIHAEKAEILASELGRLKALLGSKEEKEAIEGNEIVSKLKSE 266
Query: 407 IDILTSTIEKSKHEYENSKAEWEEKELHLVDAVKKSEEENSSLDKEIDRLVNLLKQTEEE 466
I++L +EK E+S E E L ++ ++ S + ++ N + + E+E
Sbjct: 267 IELLRGELEKVS-ILESSLKEQEGLVEQLKVDLEAAKMAESCTNSSVEEWKNKVHELEKE 326
Query: 467 ACKMREEEAQLKDSLKEVEAEVIYLQEALGEAKSESMKLKESLLDKENEFQSIHQENEE- 526
+ ++ +S++ V ++ L L E KS++ KE + E ++ + EE
Sbjct: 327 VEESNRSKSSASESMESVMKQLAELNHVLHETKSDNAAQKEKIELLEKTIEAQRTDLEEY 386
Query: 527 -----LLTREAASLKK-VDELSKLLEEASAKKQTMENGEPTDSEKDYDLLPKVVEFSEEN 586
+ EA+ L+ V+ + LE + +K + E + +LL + E S E
Sbjct: 387 GRQVCIAKEEASKLENLVESIKSELEISQEEKTRALDNEKAATSNIQNLLDQRTELSIE- 446
Query: 587 GKRQEEKTKVEPPIPIEHEEHKFEFPWVGNGASDEKTEKTDSAATLQNGNDKPKEAEKKE 646
E+ KV E E+ K + + + TE +++ ATL ++ K E
Sbjct: 447 ----LERCKV------EEEKSKKDMESLTLALQEASTESSEAKATLLVCQEELKNC---E 506
Query: 647 KEDDSVKVEYKMWESCKIEKKEFSQEGGEPEHESIDDETDS 666
+ DS+K+ K EK E E E +S+ DS
Sbjct: 507 SQVDSLKLASKETN----EKYEKMLEDARNEIDSLKSTVDS 525
BLAST of Cucsa.318540 vs. TAIR10
Match:
AT4G27595.1 (AT4G27595.1 Plant protein of unknown function (DUF827))
HSP 1 Score: 511.1 bits (1315), Expect = 1.1e-144
Identity = 309/731 (42.27%), Postives = 454/731 (62.11%), Query Frame = 1
Query: 1 MEQAGLEEAHKKEEEWEKEIEAVRSQHALDVAALLSTSQELQRVKMELAMTTDAKNQALS 60
+EQAG+E HKKE W+KE+E++RSQHALD++ALLST++EL R+K ELAMT DAKN+ALS
Sbjct: 165 LEQAGIEAVHKKEVSWKKEVESIRSQHALDISALLSTTEELHRIKQELAMTADAKNKALS 224
Query: 61 HADDATKIAEIHVEKVEILSGELTRLKALLDSKLEMQSNENGQLIMKLKSEIDSLNLELE 120
HA++ATKIAE EK EILS EL+RLKAL+ S + +SNE+ +++ KLKSEI+ L +LE
Sbjct: 225 HAEEATKIAENQAEKAEILSSELSRLKALVGSDEQKKSNEDDEVVSKLKSEIEMLRGKLE 284
Query: 121 KAKSYAEMVKEKEVSIERLNSELKAAKMAETCYEETIMDKDASIEQLNIDLEAAKMAETY 180
K VSI E T+ D++ SIE L++DL+AAKM E+Y
Sbjct: 285 K------------VSI----------------LENTLKDQEESIELLHVDLQAAKMVESY 344
Query: 181 AHGLVEEWKNRAEEMETKLDSANKLERSASESLDSVMKQLEHNNDLLHNAELEIAALKEK 240
A+ L EWKN E++ +++ + +L+ SASESLD MKQLE NN LH AEL A LKEK
Sbjct: 345 ANNLAAEWKN---EVDKQVEESKELKTSASESLDLAMKQLEENNHALHEAELGNATLKEK 404
Query: 241 VGLLEMTVKRQKEDLKESEHHLHRAKEEASEMEKLVASLRAQLETVNEEKTQALNNEKLA 300
V L T+ RQ+ DL+ES+H + +KEE S++EKLV S+++ LET EK +AL NEK A
Sbjct: 405 VESLVTTIGRQENDLEESQHQVCISKEETSKLEKLVESIKSDLETTQGEKVRALLNEKTA 464
Query: 301 ASSVQSLLEEKNQLLNELETSKDEEEKSKKAMESLASALHEISTEARETKEKLLSSQADQ 360
S +Q+LL EK +L ELE K EEEK KKAMESL L E+S EA+E KEKLL+ QA+
Sbjct: 465 TSQIQNLLSEKTELATELENCKKEEEKIKKAMESLTLDLQEVSVEAKEAKEKLLTCQAEL 524
Query: 361 ENYESQIENLKLVLKATNEKYENMLENSNHEIDILTSTIEKSKHEYENSKAEWEEKELHL 420
E QIE+LKL K TNEK+ MLE++ +EID L S++E +++E+ NSK EWE++ELHL
Sbjct: 525 ELCGVQIESLKLAEKDTNEKHGKMLEDARNEIDGLKSSLENTENEFFNSKTEWEQRELHL 584
Query: 421 VDAVKKSEEENSSLDKEIDRLVNLLKQTEEEACKMREEEAQLKDSLKEVEAEVIYLQEAL 480
+ VKK E+ N S+ +E+ ++ NLL E EAC +EE+A+++ + KE+E E+ LQE +
Sbjct: 585 MLCVKKLEDGNFSVQEELSKVKNLLHLKEVEACAAKEEDAKMQTNRKELEEEIKDLQEIV 644
Query: 481 GEAKSESMKLKESLLDKENEFQSIHQENEELLTREAASLKKVDELSKLLEEASAKKQTME 540
AK++SMKLKESL++KE+E ++ EN +L E +S+ K+D+LSK+ E K+ ++
Sbjct: 645 EVAKADSMKLKESLVEKEDELKNTAAENRKLREMEVSSIDKIDQLSKVKESLVDKETKLQ 704
Query: 541 NGEPTDSE---KDYDLLPKVVEFSEENGKRQEEKTKVEPPIPIEHEEHKFEFPWVG--NG 600
N E K+ D L K+ E S E++TK+ + E + E +
Sbjct: 705 NIIQEAEELRVKEIDYLKKIEELSAAKESLVEKETKLLSTVQEAEELRRRELACLKKIEE 764
Query: 601 ASDEKTEKTDSAATLQNGNDKPKEAEKKEKEDDSVK-VEYKMWESCKIEKKEFSQEGGEP 660
S D LQ+ + + KE+E +++K +E + ++ +KE +
Sbjct: 765 LSAVNERLVDKETKLQSSIQEVEVL--KEREAENIKQIEELSLSNERLVEKEAKLQTVVQ 824
Query: 661 EHESIDDETDSKPEGGESFDPINGVSSENLDDGGHSPSKQQEQQQQQKKKKPLLKKFGYL 720
E+E + ++ + + E ++ + + D S QE ++ ++++ LKK L
Sbjct: 825 ENEELREKESAYQKKIEELSKVDEIFA---DREAKLQSSTQENEELREREVAYLKKIEEL 859
Query: 721 LKKKNSVNQKQ 726
K + ++ K+
Sbjct: 885 AKLQENLLDKE 859
HSP 2 Score: 170.6 bits (431), Expect = 3.5e-42
Identity = 192/728 (26.37%), Postives = 329/728 (45.19%), Query Frame = 1
Query: 18 KEIEAVRSQHALDVAALLSTSQELQRVKMELAMTTDAKNQALSHADDATKIAEIHVEKVE 77
KE+EA ++ + A + + +EL+ +L + D+ K+ E VEK +
Sbjct: 581 KEVEACAAKE--EDAKMQTNRKELEEEIKDLQEIVEVAKA------DSMKLKESLVEKED 640
Query: 78 ILSGELTRLKALLDSKLEMQSNENGQLIMKLKSEIDSLNLELEKAKSYAEMVKEKEVSIE 137
L + L + +E+ S + + K+K + +L+ AE ++ KE+
Sbjct: 641 ELKNTAAENRKLRE--MEVSSIDKIDQLSKVKESLVDKETKLQNIIQEAEELRVKEIDYL 700
Query: 138 RLNSELKAAKMAETCYEETIMDKDASIEQLNIDLEAAKMAETYAHGLVEEWKNRAEEMET 197
+ EL AAK E++++K+ + + E + E +EE E +
Sbjct: 701 KKIEELSAAK-------ESLVEKETKLLSTVQEAEELRRRELACLKKIEELSAVNERL-- 760
Query: 198 KLDSANKLERSASESLDSVMKQLEHNN-DLLHNAELEIAALKEKVGLLEMTVKRQKEDLK 257
+D KL+ S E V+K+ E N + L L EK L+ TV ++ E+L+
Sbjct: 761 -VDKETKLQSSIQEV--EVLKEREAENIKQIEELSLSNERLVEKEAKLQ-TVVQENEELR 820
Query: 258 ESEHHLHRAKEEASEMEKLVASLRAQLETVNEEKTQALNNEKLAASSVQSLLEEKNQLLN 317
E E + EE S+++++ A A+L++ +E + E ++ L + + LL+
Sbjct: 821 EKESAYQKKIEELSKVDEIFADREAKLQSSTQENEELREREVAYLKKIEELAKLQENLLD 880
Query: 318 ELETSKDE--EEKSKKAMESLASALHEISTEARETKEKLLSSQADQENYESQIENLK--- 377
+ D E + KA +SLA E E + LL +++ ++ + E LK
Sbjct: 881 KENELHDMVLEIEDLKAKDSLAEKKIE---ELSNLNKSLLVKESELQDVVFENEKLKSKE 940
Query: 378 -LVLKATNEKYENMLENSNHEIDILTSTIEKSK------------HEYENSKAEWEEKEL 437
L LK T E + ++ E ++ T+ +E K E +N K +KE
Sbjct: 941 ALSLKTTEELSDVKQTLADKEKELKTAVVENEKLKAQAASSFQKIEELKNLKQSLLDKEN 1000
Query: 438 HLVDAVKKSEE----ENSSLDKEIDRLVNLLKQTEEEACKMREEEAQLKDSLKEVEAEVI 497
L + +EE E SSL K+ID L++L + ++ + +E + + + K +E
Sbjct: 1001 ELEGVFQANEELKAKEASSL-KKIDELLHLEQSWIDKGNENQELKVREASAAKRIE---- 1060
Query: 498 YLQEALGEAKSESMKLKESLLDKENEFQSIHQENEELLTREAASLKKVDELSKLLEEASA 557
E K+KESLLDKE Q++ +N EL REA++LKK++ELSKLLEEAS+
Sbjct: 1061 -----------ELSKMKESLLDKE--LQTVIHDNYELKAREASALKKIEELSKLLEEASS 1120
Query: 558 KKQTMENGEPTDSEKDYDLLPKVVEFSEENGKRQEEKTKVEPPIPIEHEEHKFEFPWVGN 617
T E GE + +D G+++ +++ +E
Sbjct: 1121 ---THEKGEEITNTNPFD---------NSTGEQKVQESPLE------------------- 1180
Query: 618 GASDEKTEKTDSAATLQNGNDKPKEAEKKEKEDDSVKVEYKMWESCKIEKKEFSQEGGEP 677
+ ++ K D+ N + +K K+ D+V+ E E + + +
Sbjct: 1181 --AIDRHLKDDTTIHWSAHNVQVIGKGEKGKDKDTVESEVYHLEKREASSER------DT 1218
Query: 678 EHESIDDETDSKPEGGESFDPI-NGVS-SENLDDGGHSPSKQQEQQQQQKKKKPLLKKFG 721
EH+ ++E DSK EG E+FD + NG+S +E +D + QQQKKKKPLL+KFG
Sbjct: 1241 EHDFAEEEVDSKAEGSENFDQLSNGLSLAEQTED-------VVSKDQQQKKKKPLLRKFG 1218
HSP 3 Score: 116.7 bits (291), Expect = 6.0e-26
Identity = 181/740 (24.46%), Postives = 308/740 (41.62%), Query Frame = 1
Query: 17 EKEIEAVRSQHALDVAALLSTSQELQRVKMELAMTTDAKNQALSHADDATKIAEIHVEKV 76
E ++E + Q + + ++ +K +L T K +AL + AT ++
Sbjct: 385 ENDLEESQHQVCISKEETSKLEKLVESIKSDLETTQGEKVRALLNEKTATS-------QI 444
Query: 77 EILSGELTRLKALLDSKLEMQSNENGQLIMKLKSEIDSLNLELEKAKSYAEMVKEK---- 136
+ L E T L L++ + + K+K ++SL L+L++ A+ KEK
Sbjct: 445 QNLLSEKTELATELENCKKEEE--------KIKKAMESLTLDLQEVSVEAKEAKEKLLTC 504
Query: 137 EVSIERLNSELKAAKMAETCYEET----IMDKDASIEQLNIDLEAAKMAETYAHGLVEEW 196
+ +E ++++ K+AE E + D I+ L LE E EW
Sbjct: 505 QAELELCGVQIESLKLAEKDTNEKHGKMLEDARNEIDGLKSSLENT---ENEFFNSKTEW 564
Query: 197 KNRAEEMETKLDSANKLERSASESLDSVMKQLEHNNDLLHNAELEIAALKEKVGLLEMTV 256
+ R E+ L KLE + SV ++L +LLH E+E A KE+ ++
Sbjct: 565 EQR--ELHLML-CVKKLE----DGNFSVQEELSKVKNLLHLKEVEACAAKEEDAKMQTNR 624
Query: 257 KRQKEDLKESEHHLHRAKEEASEMEKLVASLRAQLETVNEEKTQALNNEKLAASSVQSLL 316
K +E++K+ + + AK ++ ++++ + +E +E K A N KL V S+
Sbjct: 625 KELEEEIKDLQEIVEVAKADSMKLKESL------VEKEDELKNTAAENRKLREMEVSSI- 684
Query: 317 EEKNQLLNELETSKDEEEKSKKAMESLASALHEISTEARETKEKLLSSQADQENYESQIE 376
++ +QL E+ D+E K L I EA E + K + +Y +IE
Sbjct: 685 DKIDQLSKVKESLVDKETK-----------LQNIIQEAEELRVKEI-------DYLKKIE 744
Query: 377 NLKLVLKATNEKYENMLENSNHEIDILTSTIEKSKHEYENSKAEWEEKELHLVDAVKKSE 436
L ++ EK +L ST+++++ E +EL + +++
Sbjct: 745 ELSAAKESLVEKETKLL-----------STVQEAE--------ELRRRELACLKKIEELS 804
Query: 437 EENSSL-DKEID-----RLVNLLKQTEEEACKMREEEAQLKDSLKEVEAE---VIYLQEA 496
N L DKE + V +LK+ E E K EE + + L E EA+ V+ E
Sbjct: 805 AVNERLVDKETKLQSSIQEVEVLKEREAENIKQIEELSLSNERLVEKEAKLQTVVQENEE 864
Query: 497 LGEAKS-------ESMKLKESLLDKENEFQSIHQENEELLTREAASLKKVDELSKLLEEA 556
L E +S E K+ E D+E + QS QENEEL RE A LKK++EL+KL E
Sbjct: 865 LREKESAYQKKIEELSKVDEIFADREAKLQSSTQENEELREREVAYLKKIEELAKLQENL 924
Query: 557 SAKKQTMENG--EPTDSEKDYDLLPKVVEFSEENGKRQEEKTKVEPPIPIEHEEHKFEFP 616
K+ + + E D + L K +E K K + E+E+ K +
Sbjct: 925 LDKENELHDMVLEIEDLKAKDSLAEKKIEELSNLNKSLLVKESELQDVVFENEKLKSK-- 984
Query: 617 WVGNGASDEKTEK-TDSAATLQNGNDKPKEAEKKEKEDDSVKVEYKMWESCKIEKKEFSQ 676
S + TE+ +D TL DK KE + E++ +K + E K Q
Sbjct: 985 ---EALSLKTTEELSDVKQTLA---DKEKELKTAVVENEKLKAQAASSFQKIEELKNLKQ 1042
Query: 677 EGGEPEHESIDDETDSKPEGGESFDPINGVSSENLDDGGHSP----SKQQEQQQQQKKKK 726
+ E +E + + E S + +D+ H K E Q+ + ++
Sbjct: 1045 SLLDKE-----NELEGVFQANEELKAKEASSLKKIDELLHLEQSWIDKGNENQELKVREA 1042
HSP 4 Score: 94.0 bits (232), Expect = 4.1e-19
Identity = 172/731 (23.53%), Postives = 302/731 (41.31%), Query Frame = 1
Query: 9 AHKKEEEWEKEIEAVRSQHALDVAALLSTSQELQRVKMELAMTTDAKNQALSHADDATKI 68
A+ EW+ E++ + + + ++ S S +L ++E N AL A+
Sbjct: 317 ANNLAAEWKNEVDK-QVEESKELKTSASESLDLAMKQLE------ENNHALHEAELGNAT 376
Query: 69 AEIHVEKVEILSGELTRLKALLDSKLEMQ--SNENGQLIMKLKSEIDSLNLELEKAKSYA 128
+ EKVE L + R + L+ S E + KL I S +LE + +
Sbjct: 377 LK---EKVESLVTTIGRQENDLEESQHQVCISKEETSKLEKLVESIKS-DLETTQGEKVR 436
Query: 129 EMVKEKEVS--IERLNSE-LKAAKMAETCYEETIMDKDASIEQLNIDLEAAKMAETYAHG 188
++ EK + I+ L SE + A E C +E K A +E L +DL+ + A
Sbjct: 437 ALLNEKTATSQIQNLLSEKTELATELENCKKEEEKIKKA-MESLTLDLQEVSVEAKEAKE 496
Query: 189 LVEEWKNRAEEMETKLDSANKLERSASESLDSVMKQLEHNNDLLHNAELEIAALKEKVGL 248
+ + E +++S E+ +E + +L +A EI LK
Sbjct: 497 KLLTCQAELELCGVQIESLKLAEKDTNEK----------HGKMLEDARNEIDGLKSS--- 556
Query: 249 LEMTVKRQKEDLKESEHHLHRAKEEASEMEKLVASLRAQLETVNEEKTQALNNEKLAASS 308
L+ +E+ +K E + E + +LE N + L S
Sbjct: 557 -----------LENTENEFFNSKTEWEQRELHLMLCVKKLEDGNFSVQEEL-------SK 616
Query: 309 VQSLLEEKNQLLNELETSKDEEEKSK-----KAMESLASALHEISTEARETKEKLLSSQA 368
V++LL L E+E +EE +K K +E L EI A+ KL S
Sbjct: 617 VKNLLH-----LKEVEACAAKEEDAKMQTNRKELEEEIKDLQEIVEVAKADSMKLKESLV 676
Query: 369 DQENYESQIENLKLVLKATNEKYENMLENSNHEIDILTSTIEK---SKHEYENSKAEWEE 428
++E+ +++N A N K M +S +ID L+ E + + +N E EE
Sbjct: 677 EKED---ELKNTA----AENRKLREMEVSSIDKIDQLSKVKESLVDKETKLQNIIQEAEE 736
Query: 429 KELHLVDAVKKSEEENSSLDKEIDRLVNLLKQTEEEACKMREEEAQLKDSLKEVEAEVIY 488
+ +D +KK EE +++ + +++ LL T +EA ++R E ++E+ A
Sbjct: 737 LRVKEIDYLKKIEELSAAKESLVEKETKLLS-TVQEAEELRRRELACLKKIEELSA---- 796
Query: 489 LQEALGEAKSESMKLKESLLDKENEFQSIHQENEELLTREAASLKKVDELS---KLLEEA 548
+ E L+DKE + QS QE E L REA ++K+++ELS + L E
Sbjct: 797 --------------VNERLVDKETKLQSSIQEVEVLKEREAENIKQIEELSLSNERLVEK 856
Query: 549 SAKKQTM--ENGEPTDSEKDY----DLLPKVVE-FSEENGKRQEEKTKVEP--PIPIEHE 608
AK QT+ EN E + E Y + L KV E F++ K Q + E + +
Sbjct: 857 EAKLQTVVQENEELREKESAYQKKIEELSKVDEIFADREAKLQSSTQENEELREREVAYL 916
Query: 609 EHKFEFPWVGNGASDEKTEKTDSAATLQNGNDKPKEAEKKEKEDDSVKVEYKMWESCKIE 668
+ E + D++ E D +++ K AEKK +E ++ + ES +++
Sbjct: 917 KKIEELAKLQENLLDKENELHDMVLEIEDLKAKDSLAEKKIEELSNLNKSLLVKES-ELQ 972
Query: 669 KKEFSQEGGEPEH----ESIDDETDSKPEGGESFDPINGVSSENLDDGGHSPSKQQEQQQ 711
F E + + ++ ++ +D K + + EN + S Q+ ++
Sbjct: 977 DVVFENEKLKSKEALSLKTTEELSDVKQTLADKEKELKTAVVENEKLKAQAASSFQKIEE 972
BLAST of Cucsa.318540 vs. TAIR10
Match:
AT4G40020.1 (AT4G40020.1 Myosin heavy chain-related protein)
HSP 1 Score: 172.9 bits (437), Expect = 7.0e-43
Identity = 135/489 (27.61%), Postives = 237/489 (48.47%), Query Frame = 1
Query: 206 ERSASESLDSVMKQLEHNNDLLHNAELEIAALKEKVGLLEMTVKRQKEDLKESEHHLHRA 265
E+ +S ++LE L+ +++EIA+LKEK+ + +ED
Sbjct: 64 EKKLLDSFKDQSRELEETKALVEESKVEIASLKEKIDTSYNSQDSSEED----------- 123
Query: 266 KEEASEMEKLVASLRAQLETVNEEKTQALNNEKLAASSVQSLLEEKNQLLNELETSKDEE 325
++++S + + SL+ ++E+ E QA + ++ V LLEE + NEL+++ D E
Sbjct: 124 EDDSSVQDFDIESLKTEMESTKESLAQAHEAAQASSLKVSELLEEMKSVKNELKSATDAE 183
Query: 326 EKSKKAMESLASALHEISTEARETKEKLLSSQADQENYESQIENLKLVLKATNEKYENML 385
++KAM+ LA AL E++T+ +TKEKL+ E+++E ++ + +KYE +
Sbjct: 184 MTNEKAMDDLALALKEVATDCSQTKEKLVI-------VETELEAARIESQQWKDKYEEVR 243
Query: 386 ENSNHEIDILTSTIEKSKHEYENSKAEWEEKELHLVDAVKKSEEENSSLDKEIDRLVNLL 445
+++ ++L +T E+ + E E S W KE V +K+ E+E +SL E +RL+ L
Sbjct: 244 KDA----ELLKNTSERLRIEAEESLLAWNGKESVFVTCIKRGEDEKNSLLDENNRLLEAL 303
Query: 446 KQTEEEACKMREEEAQLKDSLKEVEAEVIYLQEALGEAKSESMKLKESLLDKENEFQSIH 505
E + K +EE +++D LK+ E +EA G A++E+ LK++LLDKE E Q
Sbjct: 304 VAAENLSKKAKEENHKVRDILKQAINEANVAKEAAGIARAENSNLKDALLDKEEELQFAL 363
Query: 506 QENEELLTREAASLKKVDELSKLLEEASAKKQTMENGEPTDSEKDYDLLPKVVEFSEENG 565
+E E + EA + + +L K+L E ME + + + +VVE E
Sbjct: 364 KEIERVKVNEAVANDNIKKLKKMLSEIEV---AMEEEKQRSLNRQESMPKEVVEVVE--- 423
Query: 566 KRQEEKTKVEPPIPIEHEEHKFEFPWVGNGASDEKTEKTDSAATLQNGNDKPKEAEKKEK 625
K+ EEK K EE K + K EK +S + ++K ++ EKKE+
Sbjct: 424 KKIEEKEK--------KEEKK-----------ENKKEKKESKKEKKEHSEKKEDKEKKEQ 483
Query: 626 EDDSVKVEYKMWESCKIEKKEFSQEGGEPEHESIDDETDSKPEGGESFDPINGVSSENLD 685
+ + + ++C + + +H E ++K G G EN D
Sbjct: 484 THQNFD-KRMIGKTCSFSIMKLAHHNHNHKHNKETSEEETKNANG-------GNHQENSD 497
Query: 686 DG--GHSPS 693
+ G+SPS
Sbjct: 544 ESGEGNSPS 497
HSP 2 Score: 77.4 bits (189), Expect = 4.0e-14
Identity = 85/420 (20.24%), Postives = 186/420 (44.29%), Query Frame = 1
Query: 75 KVEILSGELTRLKALLDSKLEMQSNENGQLIMK----------LKSEIDSLNLELEKAKS 134
KV + EL ++KA L+++ + +L+ K K +DS + + +
Sbjct: 21 KVADIGTELYKMKASLENRENEVVSLKQELLKKDIFIKNLEAAEKKLLDSFKDQSRELEE 80
Query: 135 YAEMVKEKEVSIERLNSELKAAKMAETCYEETIMD---KDASIEQLNIDLEAAKMAETYA 194
+V+E +V I L ++ + ++ EE D +D IE L ++E+ K + A
Sbjct: 81 TKALVEESKVEIASLKEKIDTSYNSQDSSEEDEDDSSVQDFDIESLKTEMESTKESLAQA 140
Query: 195 HGLVEEWKNRAEEMETKLDSANKLERSASESLDSVMKQLEHNNDLLHNAELEIAALKEKV 254
H + + E+ ++ S +SA+++ + K ++ L + + KEK+
Sbjct: 141 HEAAQASSLKVSELLEEMKSVKNELKSATDAEMTNEKAMDDLALALKEVATDCSQTKEKL 200
Query: 255 GLLEMTVKRQKEDLKESEHHLHRAKEEASEMEKLVASLRAQLETV----NEEKTQALNNE 314
++E ++ + + ++ + +++A ++ LR + E N +++ +
Sbjct: 201 VIVETELEAARIESQQWKDKYEEVRKDAELLKNTSERLRIEAEESLLAWNGKESVFVTCI 260
Query: 315 KLAASSVQSLLEEKNQLLNELETSKDEEEKSKKAMESLASALHEISTEARETKEKLLSSQ 374
K SLL+E N+LL L +++ +K+K+ + L + EA KE ++
Sbjct: 261 KRGEDEKNSLLDENNRLLEALVAAENLSKKAKEENHKVRDILKQAINEANVAKEAAGIAR 320
Query: 375 ADQENYESQI----ENLKLVLKATNE-KYENMLENSN-HEIDILTSTIEKS-KHEYENSK 434
A+ N + + E L+ LK K + N N ++ + S IE + + E + S
Sbjct: 321 AENSNLKDALLDKEEELQFALKEIERVKVNEAVANDNIKKLKKMLSEIEVAMEEEKQRSL 380
Query: 435 AEWEEKELHLVDAVKKSEEENSSLDKEIDRLVNLLKQTEEEACKMREEEAQLKDSLKEVE 471
E +V+ V+K EE +++ + K+ ++E+ K ++E ++ K+ ++ E
Sbjct: 381 NRQESMPKEVVEVVEKKIEEKEKKEEKKEN-----KKEKKESKKEKKEHSEKKEDKEKKE 435
BLAST of Cucsa.318540 vs. NCBI nr
Match:
gi|449460688|ref|XP_004148077.1| (PREDICTED: WEB family protein At3g02930, chloroplastic-like [Cucumis sativus])
HSP 1 Score: 1396.3 bits (3613), Expect = 0.0e+00
Identity = 725/725 (100.00%), Postives = 725/725 (100.00%), Query Frame = 1
Query: 1 MEQAGLEEAHKKEEEWEKEIEAVRSQHALDVAALLSTSQELQRVKMELAMTTDAKNQALS 60
MEQAGLEEAHKKEEEWEKEIEAVRSQHALDVAALLSTSQELQRVKMELAMTTDAKNQALS
Sbjct: 155 MEQAGLEEAHKKEEEWEKEIEAVRSQHALDVAALLSTSQELQRVKMELAMTTDAKNQALS 214
Query: 61 HADDATKIAEIHVEKVEILSGELTRLKALLDSKLEMQSNENGQLIMKLKSEIDSLNLELE 120
HADDATKIAEIHVEKVEILSGELTRLKALLDSKLEMQSNENGQLIMKLKSEIDSLNLELE
Sbjct: 215 HADDATKIAEIHVEKVEILSGELTRLKALLDSKLEMQSNENGQLIMKLKSEIDSLNLELE 274
Query: 121 KAKSYAEMVKEKEVSIERLNSELKAAKMAETCYEETIMDKDASIEQLNIDLEAAKMAETY 180
KAKSYAEMVKEKEVSIERLNSELKAAKMAETCYEETIMDKDASIEQLNIDLEAAKMAETY
Sbjct: 275 KAKSYAEMVKEKEVSIERLNSELKAAKMAETCYEETIMDKDASIEQLNIDLEAAKMAETY 334
Query: 181 AHGLVEEWKNRAEEMETKLDSANKLERSASESLDSVMKQLEHNNDLLHNAELEIAALKEK 240
AHGLVEEWKNRAEEMETKLDSANKLERSASESLDSVMKQLEHNNDLLHNAELEIAALKEK
Sbjct: 335 AHGLVEEWKNRAEEMETKLDSANKLERSASESLDSVMKQLEHNNDLLHNAELEIAALKEK 394
Query: 241 VGLLEMTVKRQKEDLKESEHHLHRAKEEASEMEKLVASLRAQLETVNEEKTQALNNEKLA 300
VGLLEMTVKRQKEDLKESEHHLHRAKEEASEMEKLVASLRAQLETVNEEKTQALNNEKLA
Sbjct: 395 VGLLEMTVKRQKEDLKESEHHLHRAKEEASEMEKLVASLRAQLETVNEEKTQALNNEKLA 454
Query: 301 ASSVQSLLEEKNQLLNELETSKDEEEKSKKAMESLASALHEISTEARETKEKLLSSQADQ 360
ASSVQSLLEEKNQLLNELETSKDEEEKSKKAMESLASALHEISTEARETKEKLLSSQADQ
Sbjct: 455 ASSVQSLLEEKNQLLNELETSKDEEEKSKKAMESLASALHEISTEARETKEKLLSSQADQ 514
Query: 361 ENYESQIENLKLVLKATNEKYENMLENSNHEIDILTSTIEKSKHEYENSKAEWEEKELHL 420
ENYESQIENLKLVLKATNEKYENMLENSNHEIDILTSTIEKSKHEYENSKAEWEEKELHL
Sbjct: 515 ENYESQIENLKLVLKATNEKYENMLENSNHEIDILTSTIEKSKHEYENSKAEWEEKELHL 574
Query: 421 VDAVKKSEEENSSLDKEIDRLVNLLKQTEEEACKMREEEAQLKDSLKEVEAEVIYLQEAL 480
VDAVKKSEEENSSLDKEIDRLVNLLKQTEEEACKMREEEAQLKDSLKEVEAEVIYLQEAL
Sbjct: 575 VDAVKKSEEENSSLDKEIDRLVNLLKQTEEEACKMREEEAQLKDSLKEVEAEVIYLQEAL 634
Query: 481 GEAKSESMKLKESLLDKENEFQSIHQENEELLTREAASLKKVDELSKLLEEASAKKQTME 540
GEAKSESMKLKESLLDKENEFQSIHQENEELLTREAASLKKVDELSKLLEEASAKKQTME
Sbjct: 635 GEAKSESMKLKESLLDKENEFQSIHQENEELLTREAASLKKVDELSKLLEEASAKKQTME 694
Query: 541 NGEPTDSEKDYDLLPKVVEFSEENGKRQEEKTKVEPPIPIEHEEHKFEFPWVGNGASDEK 600
NGEPTDSEKDYDLLPKVVEFSEENGKRQEEKTKVEPPIPIEHEEHKFEFPWVGNGASDEK
Sbjct: 695 NGEPTDSEKDYDLLPKVVEFSEENGKRQEEKTKVEPPIPIEHEEHKFEFPWVGNGASDEK 754
Query: 601 TEKTDSAATLQNGNDKPKEAEKKEKEDDSVKVEYKMWESCKIEKKEFSQEGGEPEHESID 660
TEKTDSAATLQNGNDKPKEAEKKEKEDDSVKVEYKMWESCKIEKKEFSQEGGEPEHESID
Sbjct: 755 TEKTDSAATLQNGNDKPKEAEKKEKEDDSVKVEYKMWESCKIEKKEFSQEGGEPEHESID 814
Query: 661 DETDSKPEGGESFDPINGVSSENLDDGGHSPSKQQEQQQQQKKKKPLLKKFGYLLKKKNS 720
DETDSKPEGGESFDPINGVSSENLDDGGHSPSKQQEQQQQQKKKKPLLKKFGYLLKKKNS
Sbjct: 815 DETDSKPEGGESFDPINGVSSENLDDGGHSPSKQQEQQQQQKKKKPLLKKFGYLLKKKNS 874
Query: 721 VNQKQ 726
VNQKQ
Sbjct: 875 VNQKQ 879
BLAST of Cucsa.318540 vs. NCBI nr
Match:
gi|659118529|ref|XP_008459169.1| (PREDICTED: WEB family protein At3g02930, chloroplastic [Cucumis melo])
HSP 1 Score: 1372.8 bits (3552), Expect = 0.0e+00
Identity = 712/725 (98.21%), Postives = 721/725 (99.45%), Query Frame = 1
Query: 1 MEQAGLEEAHKKEEEWEKEIEAVRSQHALDVAALLSTSQELQRVKMELAMTTDAKNQALS 60
MEQAGLEEAHKKEEEW+KEIEAVRSQHALDV+ALLSTSQELQRVKMELAMTTDAKNQALS
Sbjct: 155 MEQAGLEEAHKKEEEWQKEIEAVRSQHALDVSALLSTSQELQRVKMELAMTTDAKNQALS 214
Query: 61 HADDATKIAEIHVEKVEILSGELTRLKALLDSKLEMQSNENGQLIMKLKSEIDSLNLELE 120
HADDATKIAEIHVEKVEILSGELTRLKALLDSKLE QSNENGQLIMKLKSEIDSLNLELE
Sbjct: 215 HADDATKIAEIHVEKVEILSGELTRLKALLDSKLETQSNENGQLIMKLKSEIDSLNLELE 274
Query: 121 KAKSYAEMVKEKEVSIERLNSELKAAKMAETCYEETIMDKDASIEQLNIDLEAAKMAETY 180
KAKSYAEMVKEKEVSIERLNSELKAAKMAETCYEETIMDKDASIEQLNIDLEAAKMAETY
Sbjct: 275 KAKSYAEMVKEKEVSIERLNSELKAAKMAETCYEETIMDKDASIEQLNIDLEAAKMAETY 334
Query: 181 AHGLVEEWKNRAEEMETKLDSANKLERSASESLDSVMKQLEHNNDLLHNAELEIAALKEK 240
AHGLVEEWKNRAEEMETKLD ANKLERSASESLDSVMKQLEHNNDLLHNAELE+AALKEK
Sbjct: 335 AHGLVEEWKNRAEEMETKLDYANKLERSASESLDSVMKQLEHNNDLLHNAELEVAALKEK 394
Query: 241 VGLLEMTVKRQKEDLKESEHHLHRAKEEASEMEKLVASLRAQLETVNEEKTQALNNEKLA 300
VGLLEMTVKRQKEDLKESEHHLHRAKEEASEMEKLVASLR+QLETVNEEKTQALNNEKLA
Sbjct: 395 VGLLEMTVKRQKEDLKESEHHLHRAKEEASEMEKLVASLRSQLETVNEEKTQALNNEKLA 454
Query: 301 ASSVQSLLEEKNQLLNELETSKDEEEKSKKAMESLASALHEISTEARETKEKLLSSQADQ 360
ASSVQSLLEEKNQLLNELETSKDEEEKSKKAMESLASALHEISTEARETKEKLLSSQA+Q
Sbjct: 455 ASSVQSLLEEKNQLLNELETSKDEEEKSKKAMESLASALHEISTEARETKEKLLSSQAEQ 514
Query: 361 ENYESQIENLKLVLKATNEKYENMLENSNHEIDILTSTIEKSKHEYENSKAEWEEKELHL 420
ENYESQIENLKLVLKATNEKYEN+LENSN EIDILTSTIEKSKHEYENSKAEWEEKELHL
Sbjct: 515 ENYESQIENLKLVLKATNEKYENILENSNREIDILTSTIEKSKHEYENSKAEWEEKELHL 574
Query: 421 VDAVKKSEEENSSLDKEIDRLVNLLKQTEEEACKMREEEAQLKDSLKEVEAEVIYLQEAL 480
VDAVKKSEEENSSL+KEIDRLVNLLKQTEEEACKMREEEAQLKDSLKEVEAEVIYLQEAL
Sbjct: 575 VDAVKKSEEENSSLEKEIDRLVNLLKQTEEEACKMREEEAQLKDSLKEVEAEVIYLQEAL 634
Query: 481 GEAKSESMKLKESLLDKENEFQSIHQENEELLTREAASLKKVDELSKLLEEASAKKQTME 540
GEAKSESMKLKESLLDKENEFQSIHQENEELLTREAASLKKVDELSKLLEEASAKKQT+E
Sbjct: 635 GEAKSESMKLKESLLDKENEFQSIHQENEELLTREAASLKKVDELSKLLEEASAKKQTVE 694
Query: 541 NGEPTDSEKDYDLLPKVVEFSEENGKRQEEKTKVEPPIPIEHEEHKFEFPWVGNGASDEK 600
NGEPTDSEKDYDLLPKVVEFSEENGKRQEEKTKVEPPIPIEHEEHKFEFPWVGNGASDEK
Sbjct: 695 NGEPTDSEKDYDLLPKVVEFSEENGKRQEEKTKVEPPIPIEHEEHKFEFPWVGNGASDEK 754
Query: 601 TEKTDSAATLQNGNDKPKEAEKKEKEDDSVKVEYKMWESCKIEKKEFSQEGGEPEHESID 660
TEKTDSAATLQNGNDKPKEAEKKEKEDDSVKVEYKMWESCKIEKKEFSQEGGEPEHESID
Sbjct: 755 TEKTDSAATLQNGNDKPKEAEKKEKEDDSVKVEYKMWESCKIEKKEFSQEGGEPEHESID 814
Query: 661 DETDSKPEGGESFDPINGVSSENLDDGGHSPSKQQEQQQQQKKKKPLLKKFGYLLKKKNS 720
DETDSKPEGGESFD INGVSSENLDDGG+SPSKQQEQQQQQKKKKPLLKKFGYLLKKKNS
Sbjct: 815 DETDSKPEGGESFDQINGVSSENLDDGGNSPSKQQEQQQQQKKKKPLLKKFGYLLKKKNS 874
Query: 721 VNQKQ 726
VNQKQ
Sbjct: 875 VNQKQ 879
BLAST of Cucsa.318540 vs. NCBI nr
Match:
gi|590639350|ref|XP_007029645.1| (Uncharacterized protein TCM_025519 [Theobroma cacao])
HSP 1 Score: 918.3 bits (2372), Expect = 8.4e-264
Identity = 498/724 (68.78%), Postives = 589/724 (81.35%), Query Frame = 1
Query: 1 MEQAGLEEAHKKEEEWEKEIEAVRSQHALDVAALLSTSQELQRVKMELAMTTDAKNQALS 60
+EQAG+E A KK+EEWEKEIE+VR+QHALDVAALLST+QELQRVK ELAMT DAKNQALS
Sbjct: 157 LEQAGIEAAQKKDEEWEKEIESVRNQHALDVAALLSTTQELQRVKQELAMTCDAKNQALS 216
Query: 61 HADDATKIAEIHVEKVEILSGELTRLKALLDSKLEMQSNENGQLIMKLKSEIDSLNLELE 120
HADDATKIAEIH EKVEILS EL RLK+LLDSK E ++NEN + +++LK+EI+SL ELE
Sbjct: 217 HADDATKIAEIHAEKVEILSAELVRLKSLLDSKRETEANENKE-VLRLKAEIESLKQELE 276
Query: 121 KAKSYAEMVKEKEVSIERLNSELKAAKMAETCYEETIMDKDASIEQLNIDLEAAKMAETY 180
KAK++ E + +M+K+A IEQLN+DLEAA+MAE+Y
Sbjct: 277 KAKTHEEKL---------------------------MMEKEAFIEQLNVDLEAARMAESY 336
Query: 181 AHGLVEEWKNRAEEMETKLDSANKLERSASESLDSVMKQLEHNNDLLHNAELEIAALKEK 240
AH +VEEWK+R EE+E +++ A KLERSASESLDSVMKQLE NN LH+AE EIAALKEK
Sbjct: 337 AHNVVEEWKSRVEELEMQIEEAKKLERSASESLDSVMKQLESNNYSLHDAESEIAALKEK 396
Query: 241 VGLLEMTVKRQKEDLKESEHHLHRAKEEASEMEKLVASLRAQLETVNEEKTQALNNEKLA 300
VGLLEMT+ RQ+ DL+ESEHH+ AKEE +E+ KLV SL++ LETV EEKTQALNNEKLA
Sbjct: 397 VGLLEMTIGRQRGDLEESEHHIKLAKEETAEVAKLVESLKSDLETVKEEKTQALNNEKLA 456
Query: 301 ASSVQSLLEEKNQLLNELETSKDEEEKSKKAMESLASALHEISTEARETKEKLLSSQADQ 360
ASSVQ+LLEEKN+L+NELE S+DEEEKSKKAMESLASALHE+S EARE KEKLLSS+ +
Sbjct: 457 ASSVQTLLEEKNKLINELENSRDEEEKSKKAMESLASALHEVSAEAREAKEKLLSSETEH 516
Query: 361 ENYESQIENLKLVLKATNEKYENMLENSNHEIDILTSTIEKSKHEYENSKAEWEEKELHL 420
ENYE+QIE+L+LVLKATNEKYE ML+++ + ID+LT+TIE+SK+EY+NSK EWE+KELHL
Sbjct: 517 ENYETQIEDLRLVLKATNEKYETMLDDAKNGIDLLTNTIEQSKNEYQNSKTEWEQKELHL 576
Query: 421 VDAVKKSEEENSSLDKEIDRLVNLLKQTEEEACKMREEEAQLKDSLKEVEAEVIYLQEAL 480
V+ VK+SEEENSSL+KEI+RLVNLLKQTEEEAC +EEEAQLK+SLKEVE+EVIYLQEAL
Sbjct: 577 VNCVKESEEENSSLEKEINRLVNLLKQTEEEACASKEEEAQLKESLKEVESEVIYLQEAL 636
Query: 481 GEAKSESMKLKESLLDKENEFQSIHQENEELLTREAASLKKVDELSKLLEEASAKKQTME 540
E K+ESMKLKESLLDKE E Q + QENEEL REAASLKK++ELSKLLEEA+ K+Q+ E
Sbjct: 637 KEVKTESMKLKESLLDKETELQGVIQENEELRAREAASLKKMEELSKLLEEATMKRQSEE 696
Query: 541 NGEPTDSEKDYDLLPKVVEFSEENGKRQEEKTKVEPPIPIEHEEHKFEFPWVGNGASDEK 600
NGE TDSEKDYDLLPKVVEFSEENG EEK K+E P + EE K E N S ++
Sbjct: 697 NGELTDSEKDYDLLPKVVEFSEENGHGSEEKPKLELPSE-QPEEPKKENSLEVNDVSKDE 756
Query: 601 TEKTDSAATLQNGNDKPKEAEKKEKEDDSVKVEYKMWESCKIEKKEFSQEGGEPEHESID 660
+TD A ++N N K KE E K KEDDSV+VE+KMWESCKIEKKEFS E EPE E +
Sbjct: 757 ALQTD-GAKVENVNGKLKEDESKGKEDDSVEVEFKMWESCKIEKKEFSPE-REPEQEFFE 816
Query: 661 DETDSKPEGGESFDPINGVSSENLDDGGHSPSKQQEQQQQQKKKKPLLKKFGYLLKKKNS 720
+E +SK G E FD ING+ +E++DDGG+SPSK QQQQKKKKPLL+KFG LLKKK S
Sbjct: 817 EEVESKVVGSEGFDQINGL-TESIDDGGNSPSK----QQQQKKKKPLLRKFGSLLKKKGS 844
Query: 721 VNQK 725
N K
Sbjct: 877 SNHK 844
BLAST of Cucsa.318540 vs. NCBI nr
Match:
gi|566200009|ref|XP_002319250.2| (hypothetical protein POPTR_0013s07650g [Populus trichocarpa])
HSP 1 Score: 912.5 bits (2357), Expect = 4.6e-262
Identity = 493/726 (67.91%), Postives = 586/726 (80.72%), Query Frame = 1
Query: 1 MEQAGLEEAHKKEEEWEKEIEAVRSQHALDVAALLSTSQELQRVKMELAMTTDAKNQALS 60
+EQAG+E+A KKEEEW+KE+EAVRSQHALDV ALLST+QELQR+K ELAM TDAKNQALS
Sbjct: 160 LEQAGIEDAQKKEEEWQKELEAVRSQHALDVTALLSTTQELQRLKQELAMITDAKNQALS 219
Query: 61 HADDATKIAEIHVEKVEILSGELTRLKALLDSKLEMQSNENGQLIMKLKSEIDSLNLELE 120
HADDATKIAEIH EKVE+LS ELTRL LLDSKLE ++ E+ +++++L EIDSL +LE
Sbjct: 220 HADDATKIAEIHAEKVEMLSSELTRLNVLLDSKLETEAIESNKIVLQLNEEIDSLKQQLE 279
Query: 121 KAKSYAEMVKEKEVSIERLNSELKAAKMAETCYEETIMDKDASIEQLNIDLEAAKMAETY 180
K++ + E+ +++++A IEQLN++LEAAKMAE+Y
Sbjct: 280 KSEDF----------------------------EDKLIEREAFIEQLNVELEAAKMAESY 339
Query: 181 AHGLVEEWKNRAEEMETKLDSANKLERSASESLDSVMKQLEHNNDLLHNAELEIAALKEK 240
A LVEEWKNR EE+E + + ANKLERSASESL SVMKQLE NNDLLH+AE EIAALKEK
Sbjct: 340 ACNLVEEWKNRVEELEMQAEEANKLERSASESLGSVMKQLEANNDLLHDAETEIAALKEK 399
Query: 241 VGLLEMTVKRQKEDLKESEHHLHRAKEEASEMEKLVASLRAQLETVNEEKTQALNNEKLA 300
VGLLEMT++RQK DL+ESEH L KEEAS M K V SL ++LETV EEK QALNNEKLA
Sbjct: 400 VGLLEMTIRRQKGDLEESEHSLGMVKEEASVMVKKVESLMSELETVKEEKAQALNNEKLA 459
Query: 301 ASSVQSLLEEKNQLLNELETSKDEEEKSKKAMESLASALHEISTEARETKEKLLSSQADQ 360
ASSVQSLLEEKN+L+ ELE S+DEEEKSKKAMESLASALHE+S EARE KE+L+S+Q +
Sbjct: 460 ASSVQSLLEEKNKLITELENSRDEEEKSKKAMESLASALHEVSAEAREAKERLVSNQVEH 519
Query: 361 ENYESQIENLKLVLKATNEKYENMLENSNHEIDILTSTIEKSKHEYENSKAEWEEKELHL 420
ENYE+QIE+L+LVLKATNEKYE +L+++ HEID+L +T+E+SK++++NSKAEW++KE +L
Sbjct: 520 ENYETQIEDLRLVLKATNEKYETVLDDAKHEIDLLRNTVEESKNQFQNSKAEWDQKEKNL 579
Query: 421 VDAVKKSEEENSSLDKEIDRLVNLLKQTEEEACKMREEEAQLKDSLKEVEAEVIYLQEAL 480
+ ++KSEEENSSL+KEIDRLVNLL TEEEAC MR+EEA LKDSLKEVEAEVI LQEAL
Sbjct: 580 GNYLRKSEEENSSLEKEIDRLVNLLTHTEEEACGMRDEEAHLKDSLKEVEAEVISLQEAL 639
Query: 481 GEAKSESMKLKESLLDKENEFQSIHQENEELLTREAASLKKVDELSKLLEEASAKKQTME 540
GEA+ ESMKLKESLLDKENEFQ+I QENEEL T+EA+S KKV+ELSKLLEEA AKKQ E
Sbjct: 640 GEARVESMKLKESLLDKENEFQNIFQENEELRTKEASSHKKVEELSKLLEEAMAKKQVEE 699
Query: 541 NGEPTDSEKDYDLLPKVVEFSEENGKRQEEKTKVEPPIPIEHEEHKFEFPWVGNGASDEK 600
NGE TDSEKDYDLLPKVVEFSEENG +EEK +E P+ + +E + NGA+++
Sbjct: 700 NGELTDSEKDYDLLPKVVEFSEENGHVREEKPTMELPLQLSNELNTENAQEQINGATNKA 759
Query: 601 TEKTDSAATLQNGNDKPKEAEKKEKEDDSVKVEYKMWESCKIEKKEFSQEGGEPEHE-SI 660
+ A L+N N PKE E KEKEDDSV+VE+KMWESCKIEKKEFS E E EHE S
Sbjct: 760 AQM--DAHKLENVNGNPKEDESKEKEDDSVEVEFKMWESCKIEKKEFSPE-RETEHESSF 819
Query: 661 DDETDSKPEGGESFDPINGVSS-ENLDDGGHSPSKQQEQQQQQKKKKPLLKKFGYLLKKK 720
+DE DSK +GGESFD ING+SS EN+DDGG SPSK QQQQKKKKPLL+KF LLKKK
Sbjct: 820 EDEADSKVDGGESFDQINGLSSTENVDDGGSSPSK----QQQQKKKKPLLRKFSNLLKKK 850
Query: 721 NSVNQK 725
+ NQK
Sbjct: 880 GTSNQK 850
BLAST of Cucsa.318540 vs. NCBI nr
Match:
gi|802585091|ref|XP_012070237.1| (PREDICTED: WEB family protein At5g16730, chloroplastic-like [Jatropha curcas])
HSP 1 Score: 904.4 bits (2336), Expect = 1.3e-259
Identity = 491/725 (67.72%), Postives = 585/725 (80.69%), Query Frame = 1
Query: 1 MEQAGLEEAHKKEEEWEKEIEAVRSQHALDVAALLSTSQELQRVKMELAMTTDAKNQALS 60
+EQAG+E A KKE+EW+KE+E+VR+QHALDVAALLS +QELQRVK ELAMTTDAKNQALS
Sbjct: 155 LEQAGIEAAKKKEDEWQKELESVRNQHALDVAALLSATQELQRVKQELAMTTDAKNQALS 214
Query: 61 HADDATKIAEIHVEKVEILSGELTRLKALLDSKLEMQSNENGQLIMKLKSEIDSLNLELE 120
HADDATKIAEIH +KVEILS ELTRLKALLDSK EM++NE+ +++M+LK EI++L EL+
Sbjct: 215 HADDATKIAEIHADKVEILSAELTRLKALLDSKHEMEANESNKIVMQLKEEIETLKQELK 274
Query: 121 KAKSYAEMVKEKEVSIERLNSELKAAKMAETCYEETIMDKDASIEQLNIDLEAAKMAETY 180
+AK E +++K+ASIEQLN++LEAAKMAE+Y
Sbjct: 275 RAKGV----------------------------ENELIEKEASIEQLNVELEAAKMAESY 334
Query: 181 AHGLVEEWKNRAEEMETKLDSANKLERSASESLDSVMKQLEHNNDLLHNAELEIAALKEK 240
A LV EWK R EE+E +++ ANKLERSASESL SVMKQLE NNDLLH+AE EI+ALKEK
Sbjct: 335 ARNLVAEWKCRIEELEMQVEEANKLERSASESLCSVMKQLEGNNDLLHDAESEISALKEK 394
Query: 241 VGLLEMTVKRQKEDLKESEHHLHRAKEEASEMEKLVASLRAQLETVNEEKTQALNNEKLA 300
+GLLEMT+ RQK DL+ESE L AKEE SEM K V SL+++L+ V EEK QALNNEKLA
Sbjct: 395 LGLLEMTITRQKGDLEESECCLSAAKEETSEMAKKVESLKSELDIVKEEKAQALNNEKLA 454
Query: 301 ASSVQSLLEEKNQLLNELETSKDEEEKSKKAMESLASALHEISTEARETKEKLLSSQADQ 360
ASSVQSLLEEKN+L+NELE S++EEEKSKKAMESLASALHE+S EARE KE+LLS+Q +
Sbjct: 455 ASSVQSLLEEKNKLINELENSREEEEKSKKAMESLASALHEVSAEAREAKEQLLSTQVEH 514
Query: 361 ENYESQIENLKLVLKATNEKYENMLENSNHEIDILTSTIEKSKHEYENSKAEWEEKELHL 420
ENYE+Q+ENL+LVLK NE+YE +L+++ HEI++L + IE SK+E++NSK EWE+KE +L
Sbjct: 515 ENYETQVENLRLVLKEANERYETILDDAKHEIEVLKNDIEDSKNEFQNSKDEWEQKEQNL 574
Query: 421 VDAVKKSEEENSSLDKEIDRLVNLLKQTEEEACKMREEEAQLKDSLKEVEAEVIYLQEAL 480
+D VK+SEE+NSSL++EIDRLVNLLK TEEEAC REEEA LKDSLKEVEAEVI LQE L
Sbjct: 575 IDCVKQSEEKNSSLEREIDRLVNLLKHTEEEACASREEEALLKDSLKEVEAEVISLQEGL 634
Query: 481 GEAKSESMKLKESLLDKENEFQSIHQENEELLTREAASLKKVDELSKLLEEASAKKQTME 540
GE++ ESMKLK+SLLDKENE Q++ QENEEL TREA SLKKV++LSKLLEEA AKKQT E
Sbjct: 635 GESRVESMKLKDSLLDKENELQNLIQENEELRTREAISLKKVEDLSKLLEEAIAKKQTEE 694
Query: 541 NGEPTDSEKDYDLLPKVVEFSEENGKRQEEKTKVEPPIPIEHEEHKFEFPWVGNGASDEK 600
NGE TDSEK+YDLLPKVVEFSEENG +EEK KVE P +HE+ E P N +
Sbjct: 695 NGELTDSEKEYDLLPKVVEFSEENGHVREEKPKVELP-QQQHEDVGKENPKEQNNGFNND 754
Query: 601 TEKTDSAATLQNGNDKPKEAEKKEKEDDSVKVEYKMWESCKIEKKEFSQEGGEPEHESID 660
T + AA ++NGN KPKE E KEKED+SV+ E+KMWESCKIEKKEFS E E EHES +
Sbjct: 755 TAPIE-AAKVENGNGKPKEDETKEKEDESVE-EFKMWESCKIEKKEFSPE-REHEHESFE 814
Query: 661 DETDSKPEGGESFDPINGVSS-ENLDDGGHSPSKQQEQQQQQKKKKPLLKKFGYLLKKKN 720
DE DSK EGGESFD +NG+SS EN+DDG SPSK QQQQKKKKPLL+KFG LLKKK+
Sbjct: 815 DEVDSKAEGGESFDQVNGLSSVENVDDGATSPSK----QQQQKKKKPLLRKFGSLLKKKS 843
Query: 721 SVNQK 725
+ NQK
Sbjct: 875 TSNQK 843
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| Y5673_ARATH | 5.2e-189 | 52.06 | WEB family protein At5g16730, chloroplastic OS=Arabidopsis thaliana GN=At5g16730... | [more] |
| Y3293_ARATH | 1.9e-183 | 51.86 | WEB family protein At3g02930, chloroplastic OS=Arabidopsis thaliana GN=At3g02930... | [more] |
| Y1501_ARATH | 7.6e-148 | 43.66 | Putative WEB family protein At1g65010, chloroplastic OS=Arabidopsis thaliana GN=... | [more] |
| Y4759_ARATH | 1.9e-143 | 42.27 | WEB family protein At4g27595, chloroplastic OS=Arabidopsis thaliana GN=At4g27595... | [more] |
| USO1_YEAST | 1.5e-31 | 23.38 | Intracellular protein transport protein USO1 OS=Saccharomyces cerevisiae (strain... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A0A0LRJ0_CUCSA | 0.0e+00 | 100.00 | Uncharacterized protein OS=Cucumis sativus GN=Csa_2G442260 PE=4 SV=1 | [more] |
| A0A061F0H8_THECC | 5.8e-264 | 68.78 | Uncharacterized protein OS=Theobroma cacao GN=TCM_025519 PE=4 SV=1 | [more] |
| B9I744_POPTR | 3.2e-262 | 67.91 | Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0013s07650g PE=4 SV=2 | [more] |
| A0A067L4V1_JATCU | 8.7e-260 | 67.72 | Uncharacterized protein OS=Jatropha curcas GN=JCGZ_02555 PE=4 SV=1 | [more] |
| B9S250_RICCO | 5.6e-259 | 67.08 | ATP binding protein, putative OS=Ricinus communis GN=RCOM_1327430 PE=4 SV=1 | [more] |
