Cucsa.310180 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.310180 vs. Swiss-Prot
Match: SYT4_ARATH (Synaptotagmin-4 OS=Arabidopsis thaliana GN=SYT4 PE=2 SV=1)

HSP 1 Score: 818.1 bits (2112), Expect = 6.1e-236
Identity = 405/596 (67.95%), Postives = 481/596 (80.70%), Query Frame = 1

Query: 1   MSFFSGIFLGVVVGVLLVIAFARAGNARAKHRSDLATTIAAFARMTAQDSRKILPKEFYP 60
           M F  G+F+G+ V   LV+AFAR  + R+  R+DLA TIAAFARMT QDSRK+LP +FYP
Sbjct: 1   MGFLFGLFIGIAVSFGLVVAFARYSSVRSTRRADLAKTIAAFARMTVQDSRKLLPGDFYP 60

Query: 61  SWVVFTQRQKLTWLNLQLDKIWPYVDAAASELIRSNVEPVLEEFRPVILSSLKFSKLTLG 120
           SWVVF+QRQKL WLNL+L+KIWPYV+ AASELI+S+VEPVLE++ P +L+SLKFSK TLG
Sbjct: 61  SWVVFSQRQKLNWLNLELEKIWPYVNEAASELIKSSVEPVLEQYTPAMLASLKFSKFTLG 120

Query: 121 TVAPSFTGISVLEDEPDTGGITLELEMQWDGNPNIVLDIKTKLGVSLPVQVKDIAFTGLF 180
           TVAP FTG+S+LE E    GIT+ELEMQWDGNP IVLD+KT LGVSLP++VK+I FTG+F
Sbjct: 121 TVAPQFTGVSILESESGPNGITMELEMQWDGNPKIVLDVKTLLGVSLPIEVKNIGFTGVF 180

Query: 181 RLIFKPLVDEFPCFGAVCYSLRKKKNLDFKLKIIGGDISSIPGVSDAIEETIRDAIEGTI 240
           RLIFKPLVDEFPCFGA+ YSLR+KK LDF LK+IGG+++SIPG+SDAIEETIRDAIE +I
Sbjct: 181 RLIFKPLVDEFPCFGALSYSLREKKGLDFTLKVIGGELTSIPGISDAIEETIRDAIEDSI 240

Query: 241 TWPVRKIVPILAGDYSDLEVKPVGTLEVKLVQAKELTNKDIIGKSDPYAVLFVRPLKERM 300
           TWPVRKI+PIL GDYSDLE+KPVG L+VK+VQAK+L NKD+IGKSDPYA++F+RPL +R 
Sbjct: 241 TWPVRKIIPILPGDYSDLELKPVGKLDVKVVQAKDLANKDMIGKSDPYAIVFIRPLPDRT 300

Query: 301 KTSKTINNQLNPIWNEHFNFIVEDASTQHLTIRVFDDEGVQASELIGCAQVALKDLEPGK 360
           K +KTI+N LNPIWNEHF FIVED STQHLT+RVFDDEGV +S+LIG AQV L +L PGK
Sbjct: 301 KKTKTISNSLNPIWNEHFEFIVEDVSTQHLTVRVFDDEGVGSSQLIGAAQVPLNELVPGK 360

Query: 361 VKDVWLKLVKDLEIQRDNKYRGQVRNPLHRFQLNLGCIKQSNLLNFYACTQVHLELLYYP 420
           VKD+WLKLVKDLEIQRD K RGQV+                            LELLY P
Sbjct: 361 VKDIWLKLVKDLEIQRDTKNRGQVQ----------------------------LELLYCP 420

Query: 421 YGTDQSLYINPFNPDYALTSVEKALKMAPSGSEDADSGKPSSPKKRDTIVRGVLSVTVIA 480
            G +  L  NPFNPDY+LT +EK LK     S+  D  K  + KK+D IVRGVLSVTV+A
Sbjct: 421 LGKEGGLK-NPFNPDYSLTILEKVLKPESEDSDATDMKKLVTSKKKDVIVRGVLSVTVVA 480

Query: 481 AEDLPAVDFMGKADPYVVLIMKKSETKVKTRVVHDTVNPVWNQTFDFLVEDALHDMLIVE 540
           AEDLPAVDFMGKAD +VV+ +KKSETK KTRVV D++NPVWNQTFDF+VEDALHD+L +E
Sbjct: 481 AEDLPAVDFMGKADAFVVITLKKSETKSKTRVVPDSLNPVWNQTFDFVVEDALHDLLTLE 540

Query: 541 VWDHDTFGKDKLGRVIMTLTRAILEGEIQDNFPLEGAKSGRVFLHLKWAAQPMFRD 597
           VWDHD FGKDK+GRVIMTLTR +LEGE Q+ F L+GAKSG++ +HLKW  +   RD
Sbjct: 541 VWDHDKFGKDKIGRVIMTLTRVMLEGEFQEWFELDGAKSGKLCVHLKWTPRLKLRD 567

BLAST of Cucsa.310180 vs. Swiss-Prot
Match: SYT5_ARATH (Synaptotagmin-5 OS=Arabidopsis thaliana GN=SYT5 PE=2 SV=1)

HSP 1 Score: 793.1 bits (2047), Expect = 2.1e-228
Identity = 388/597 (64.99%), Postives = 482/597 (80.74%), Query Frame = 1

Query: 1   MSFFSGIFLGVVVGVLLVIAFARAGNARAKHRSDLATTIAAFARMTAQDSRKILPKEFYP 60
           M F  G+ +G++VG+ ++I F +  N+R+K RS+LA T+AAFARMT +DSRK+LP EFYP
Sbjct: 1   MGFIVGVVIGLLVGIAIIIGFVKLENSRSKLRSELANTVAAFARMTVEDSRKLLPPEFYP 60

Query: 61  SWVVFTQRQKLTWLNLQLDKIWPYVDAAASELIRSNVEPVLEEFRPVILSSLKFSKLTLG 120
           SWVVF++RQKLTWLN  L KIWPYVD AASELI+++VEPVLE++RP I++SL FSKLTLG
Sbjct: 61  SWVVFSERQKLTWLNHHLTKIWPYVDEAASELIKASVEPVLEQYRPAIVASLTFSKLTLG 120

Query: 121 TVAPSFTGISVLEDEPDTGGITLELEMQWDGNPNIVLDIKTKLGVSLPVQVKDIAFTGLF 180
           TVAP FTG+SV++   D  GITLEL+MQWDGNPNIVL +KT +GVSLP+QVK+I FTG+F
Sbjct: 121 TVAPQFTGVSVIDG--DKNGITLELDMQWDGNPNIVLGVKTLVGVSLPIQVKNIGFTGVF 180

Query: 181 RLIFKPLVDEFPCFGAVCYSLRKKKNLDFKLKIIGGDISSIPGVSDAIEETIRDAIEGTI 240
           RLIF+PLV++FPCFGAV  SLR+KK LDF LK++GGDIS+IPG+S+AIEETIRDA+E +I
Sbjct: 181 RLIFRPLVEDFPCFGAVSVSLREKKKLDFTLKVVGGDISAIPGLSEAIEETIRDAVEDSI 240

Query: 241 TWPVRKIVPILAGDYSDLEVKPVGTLEVKLVQAKELTNKDIIGKSDPYAVLFVRPLKERM 300
           TWPVRK++PI+ GDYSDLE+KPVG LEVKLVQAK LTNKD++GKSDP+A +F+RPL+E+ 
Sbjct: 241 TWPVRKVIPIIPGDYSDLELKPVGMLEVKLVQAKNLTNKDLVGKSDPFAKMFIRPLREKT 300

Query: 301 KTSKTINNQLNPIWNEHFNFIVEDASTQHLTIRVFDDEGVQASELIGCAQVALKDLEPGK 360
           K SKTINN LNPIWNEHF F+VEDASTQHL +R++DDEGVQASELIGCAQ+ L +LEPGK
Sbjct: 301 KRSKTINNDLNPIWNEHFEFVVEDASTQHLVVRIYDDEGVQASELIGCAQIRLCELEPGK 360

Query: 361 VKDVWLKLVKDLEIQRDNKYRGQVRNPLHRFQLNLGCIKQSNLLNFYACTQVHLELLYYP 420
           VKDVWLKLVKDLEIQRD K RG                            +VHLELLY P
Sbjct: 361 VKDVWLKLVKDLEIQRDTKNRG----------------------------EVHLELLYIP 420

Query: 421 YGTDQSLYINPFNPDYALTSVEKALKMAPSGSEDADSGKPSSPKKRDTIVRGVLSVTVIA 480
           YG+   + +NPF    ++TS+E+ LK     ++  D    SS K++D IVRGVLSVTVI+
Sbjct: 421 YGSGNGI-VNPFVTS-SMTSLERVLK-----NDTTDEENASSRKRKDVIVRGVLSVTVIS 480

Query: 481 AEDLPAVDFMGKADPYVVLIMKKSETKVKTRVVHDTVNPVWNQTFDFLVEDALHDMLIVE 540
           AE++P  D MGKADPYVVL MKKS  K KTRVV+D++NPVWNQTFDF+VED LHDML++E
Sbjct: 481 AEEIPIQDLMGKADPYVVLSMKKSGAKSKTRVVNDSLNPVWNQTFDFVVEDGLHDMLVLE 540

Query: 541 VWDHDTFGKDKLGRVIMTLTRAILEGEIQDNFPLEGAKSGRVFLHLKWAAQPMFRDT 598
           VWDHDTFGKD +GR I+TLTR I+E E +D +PL+ +K+G++ LHLKW AQ ++RD+
Sbjct: 541 VWDHDTFGKDYIGRCILTLTRVIMEEEYKDWYPLDESKTGKLQLHLKWMAQSIYRDS 560

BLAST of Cucsa.310180 vs. Swiss-Prot
Match: SYT1_ARATH (Synaptotagmin-1 OS=Arabidopsis thaliana GN=SYT1 PE=1 SV=2)

HSP 1 Score: 229.2 bits (583), Expect = 1.2e-58
Identity = 183/598 (30.60%), Postives = 288/598 (48.16%), Query Frame = 1

Query: 3   FFSGIFLGVVVGVLLVIAFARAGNARAKHRSDLATTIAAFARMTAQDSRKILPKEFYPSW 62
           F  GI LG+V+G +L +          + RS       A  RM        LP+   P W
Sbjct: 13  FGVGISLGLVIGYVLFVYLLPNDVKDPEIRSIADQDPKAMLRM--------LPE--IPLW 72

Query: 63  VVFTQRQKLTWLNLQLDKIWPYVDAAASELIRSNVEPVLEEFRPVI-LSSLKFSKLTLGT 122
           V      ++ W+N  L+ +WPY+D A  +  ++  +P++EE  P   + S++F  LTLG+
Sbjct: 73  VKNPDFDRVDWINRFLEYMWPYLDKAICKTAKNIAKPIIEEQIPKYKIDSVEFETLTLGS 132

Query: 123 VAPSFTGISV-LEDEPDTGGITLELEMQWDGNPNIVLDIKTKLGVSLPVQVKDIAFTGLF 182
           + P+F G+ V L DE +   + +E  ++W  NPNI++ IK   G+   VQV D+      
Sbjct: 133 LPPTFQGMKVYLTDEKE---LIMEPCLKWAANPNILVAIKA-FGLKATVQVVDLQVFAQP 192

Query: 183 RLIFKPLVDEFPCFGAVCYSLRKKKNLDFKLKIIGGDISSIPGVSDAIEETIRDAIEGTI 242
           R+  KPLV  FPCF  +  SL +K ++DF LK+ G D+ SIPG+   ++E I+D +    
Sbjct: 193 RITLKPLVPSFPCFANIYVSLMEKPHVDFGLKLGGADLMSIPGLYRFVQEQIKDQVANMY 252

Query: 243 TWPVRKIVPILAGDYSDLEVKPVGTLEVKLVQAKELTNKDIIGKSDPYAVLFVRPLKERM 302
            WP   +VPIL  D +    +PVG + VK+V+A  L  KD++G +DP+  + +   K   
Sbjct: 253 LWPKTLVVPIL--DPAKAFRRPVGIVHVKVVRAVGLRKKDLMGGADPFVKIKLSEDKIPS 312

Query: 303 KTSKTINNQLNPIWNEHFNFIVEDASTQHLTIRVFDDEGVQASELIGCAQVALKDLEPGK 362
           K +   +  LNP WNE F F V D  TQ L   V+D E V   E +G   +ALK++ P +
Sbjct: 313 KKTTVKHKNLNPEWNEEFKFSVRDPQTQVLEFSVYDWEQVGNPEKMGMNVLALKEMVPDE 372

Query: 363 VKDVWLKLVKDLEIQRD----NKYRGQVRNPLHRFQLNLGCIKQSNLLNFYACTQVHLEL 422
            K   L+L K L+   D    +KYRG                            ++ +EL
Sbjct: 373 HKAFTLELRKTLDGGEDGQPPDKYRG----------------------------KLEVEL 432

Query: 423 LYYPYGTDQSLYINPFNPDYALTSVEKALKMAPSGSEDADSGKPSSPKKRDTIVRGVLSV 482
           LY P+  ++          +  T   +A++ AP G+  A                G+L V
Sbjct: 433 LYKPFTEEE------MPKGFEET---QAVQKAPEGTPAAG---------------GMLVV 492

Query: 483 TVIAAEDLPAVDFMGKADPYVVLIMKKSETKVKTRVVHDTVNPVWNQTFDFLVED-ALHD 542
            V +AED   V+     +PYV +  K  E   KT+ V    +P WN+ F F++E+  + +
Sbjct: 493 IVHSAED---VEGKHHTNPYVRIYFKGEER--KTKHVKKNRDPRWNEEFTFMLEEPPVRE 537

Query: 543 MLIVEVWDHDT-----FGKDKLGRVIMTLTRAILEGEIQDNFPLEGAKSGRVFLHLKW 589
            L VEV    +       K+ LG V + +   +    +   F L  +K+G++ + L+W
Sbjct: 553 KLHVEVLSTSSRIGLLHPKETLGYVDIPVVDVVNNKRMNQKFHLIDSKNGKIQIELEW 537

BLAST of Cucsa.310180 vs. Swiss-Prot
Match: SYT3_ARATH (Synaptotagmin-3 OS=Arabidopsis thaliana GN=SYT3 PE=2 SV=1)

HSP 1 Score: 219.5 bits (558), Expect = 9.6e-56
Identity = 128/388 (32.99%), Postives = 211/388 (54.38%), Query Frame = 1

Query: 1   MSFFSGIFLGVVVGVLLVIAFARAGNARAKHRSDLATTIAAFARMTAQDSRKILPKEFYP 60
           + F  GI +G+++G  ++I    +       R  + T+I+            +LP    P
Sbjct: 11  IGFVIGIPIGLILGFFVLIYSQPSHQEYPPARPLVETSISVLL--------DLLPD--IP 70

Query: 61  SWVVFTQRQKLTWLNLQLDKIWPYVDAAASELIRSNVEPVLEEFRPVI-LSSLKFSKLTL 120
            W+     +++ W N  +  +WPY+D A   +IRS+V+P+  ++     + S++F  L+L
Sbjct: 71  LWMKNPDYERVDWFNKFISYMWPYLDKAVCGIIRSSVQPLFADYIGTFCIESIEFENLSL 130

Query: 121 GTVAPSFTGISVLEDEPDTGGITLELEMQWDGNPNIVLDIKTKLGVSLPVQVKDIAFTGL 180
           GT+ P+  G+   E       +  E  ++W GNPNIVL +K  L + + VQ+ D+ F  +
Sbjct: 131 GTLPPTVHGVKFYETNEKE--LLFEPSIKWAGNPNIVLVLKV-LSLRIRVQLVDLQFFAI 190

Query: 181 FRLIFKPLVDEFPCFGAVCYSLRKKKNLDFKLKIIGGDISSIPGVSDAIEETIRDAIEGT 240
            R+  KPL+  FPCFG V  SL +K ++DF LK++GGD+ SIPG+   ++ETI+  +   
Sbjct: 191 VRVALKPLLPTFPCFGMVVVSLMEKPHVDFGLKVLGGDLMSIPGLYRYVQETIKRQVSSM 250

Query: 241 ITWPVRKIVPILAGDYSDLEVKPVGTLEVKLVQAKELTNKDIIGKSDPYAVLFVRPLKER 300
             WP    +PIL    + ++ KPVG L V +++A+ L  KD++G SDPY  L +   K  
Sbjct: 251 YHWPQVLEIPILDSSTASVK-KPVGLLHVSILRARNLLKKDLLGTSDPYVKLSLTGEKLP 310

Query: 301 MKTSKTINNQLNPIWNEHFNFIVEDASTQHLTIRVFDDEGVQASELIGCAQVALKDLEPG 360
            K +      LNP WNEHF  IV+D ++Q L + VFD + V   + +G   + L+ + PG
Sbjct: 311 AKKTTIKKRNLNPEWNEHFKLIVKDPNSQVLQLEVFDWDKVGGHDRLGMQMIPLQKINPG 370

Query: 361 KVKDVWLKLVKDLEI---QRDNKYRGQV 385
           + K+  L L+K+  +     D K RG++
Sbjct: 371 ERKEFNLDLIKNSNVVMDSGDKKKRGRL 384

HSP 2 Score: 55.8 bits (133), Expect = 1.8e-06
Identity = 38/115 (33.04%), Postives = 63/115 (54.78%), Query Frame = 1

Query: 472 GVLSVTVIAAEDLPAVDFMGKADPYVVLIMKKSETKV-KTRVVHDTVNPVWNQTFDFLVE 531
           G+L V+++ A +L   D +G +DPYV L +   +    KT +    +NP WN+ F  +V+
Sbjct: 261 GLLHVSILRARNLLKKDLLGTSDPYVKLSLTGEKLPAKKTTIKKRNLNPEWNEHFKLIVK 320

Query: 532 DALHDMLIVEVWDHDTF-GKDKLGRVIMTLTRAILEGEIQDNFPLEGAKSGRVFL 585
           D    +L +EV+D D   G D+LG  ++ L + I  GE +  F L+  K+  V +
Sbjct: 321 DPNSQVLQLEVFDWDKVGGHDRLGMQMIPLQK-INPGE-RKEFNLDLIKNSNVVM 373

HSP 3 Score: 53.5 bits (127), Expect = 9.1e-06
Identity = 50/212 (23.58%), Postives = 95/212 (44.81%), Query Frame = 1

Query: 386 NPLHRFQLNLGCIKQSNLL----NFYACTQVHLELLYYPYGTDQSLYINPFNPDYALTSV 445
           NP  R + NL  IK SN++    +     ++ ++L Y P+                    
Sbjct: 354 NPGERKEFNLDLIKNSNVVMDSGDKKKRGRLEVDLRYVPFR------------------- 413

Query: 446 EKALKMAPSGSEDADSGKPSSPKKRDTIVRGVLSVTVIAAEDLPAVDFMGKADPYVVLIM 505
           E+++K      E+  S       +      G+LSV V +A+D+        ++PY V++ 
Sbjct: 414 EESIKRRKESREEKSSEDDDFLSQA-----GLLSVAVQSAKDVEGKK--KHSNPYAVVLF 473

Query: 506 KKSETKVKTRVVHDTVNPVWNQTFDFLVED-ALHDMLIVEVWDHDT----FGKDKLGRVI 565
           +    K KT+++  T +P WN+ F F +E+  + + + VEV    T      K++LG V 
Sbjct: 474 RGE--KKKTKMLKKTRDPRWNEEFQFTLEEPPVKESIRVEVMSKGTGFHFRSKEELGHVD 533

Query: 566 MTLTRAILEGEIQDNFPLEGAKSGRVFLHLKW 589
           + L   +  G I   + L  +++G + + ++W
Sbjct: 534 INLDDVVDNGRINQKYHLINSRNGIIHIEIRW 537

BLAST of Cucsa.310180 vs. Swiss-Prot
Match: SYT2_ARATH (Synaptotagmin-2 OS=Arabidopsis thaliana GN=SYT2 PE=2 SV=1)

HSP 1 Score: 209.5 bits (532), Expect = 9.9e-53
Identity = 131/387 (33.85%), Postives = 204/387 (52.71%), Query Frame = 1

Query: 1   MSFFSGIFLGVVVGVLLVIAFARAGNARAKHRSDLATTIAAFARMTAQDSRKILPKEFYP 60
           + F  G  +G+V+G  L I F        +        I     + ++    + P+   P
Sbjct: 11  IGFGFGTTIGIVIGYYLFIYFQSTDVEDPE--------IKPLVELDSETIATMFPE--IP 70

Query: 61  SWVVFTQRQKLTWLNLQLDKIWPYVDAAASELIRSNVEPVLEEFRPVI-LSSLKFSKLTL 120
            WV      ++ WLN  +  +WPY+D A  ++ +S  +P++ E  P   + S++F  LTL
Sbjct: 71  MWVKNPDFDRIDWLNKLIGHMWPYMDKAICKMAKSIAKPIIAEQIPNYKIDSVEFEMLTL 130

Query: 121 GTVAPSFTGISVLEDEPDTGGITLELEMQWDGNPNIVLDIKTKLGVSLPVQVKDIAFTGL 180
           G++ PSF G+ V     D   I +EL ++W GNPNI++  K   G+   VQV D+     
Sbjct: 131 GSLPPSFQGMKVYAT--DDKEIIMELSVKWAGNPNIIVVAKA-FGLKATVQVIDLQVYAT 190

Query: 181 FRLIFKPLVDEFPCFGAVCYSLRKKKNLDFKLKIIGGDISSIPGVSDAIEETIRDAIEGT 240
            R+  KPLV  FPCF  +  SL  K  +DF LK++G D+ +IPG+   ++E I+D +   
Sbjct: 191 PRITLKPLVPSFPCFANIFVSLMDKPQVDFGLKLLGADVMAIPGLYRFVQEIIKDQVANM 250

Query: 241 ITWPVRKIVPILAGDYSDLEVKPVGTLEVKLVQAKELTNKDIIGKSDPYAVLFVRPLKER 300
             WP    V I+  D S    KPVG L VK+++A +L  KD++G SDPY  L +   K  
Sbjct: 251 YLWPKTLNVQIM--DPSKAMKKPVGLLSVKVIKAIKLKKKDLLGGSDPYVKLTLSGDKVP 310

Query: 301 MKTSKTINNQLNPIWNEHFNFIVEDASTQHLTIRVFDDEGVQASELIGCAQVALKDLEPG 360
            K +   ++ LNP WNE F+ +V++  +Q L + V+D E V   + IG   + LKDL P 
Sbjct: 311 GKKTVVKHSNLNPEWNEEFDLVVKEPESQELQLIVYDWEQVGKHDKIGMNVIQLKDLTPE 370

Query: 361 KVKDVWLKLVKDLEIQR--DNKYRGQV 385
           + K + L+L+K +E +     K RGQ+
Sbjct: 371 EPKLMTLELLKSMEPKEPVSEKSRGQL 382

HSP 2 Score: 58.9 bits (141), Expect = 2.2e-07
Identity = 38/102 (37.25%), Postives = 55/102 (53.92%), Query Frame = 1

Query: 460 PSSPKKRDTIVRGVLSVTVIAAEDLPAVDFMGKADPYVVLIMKKSETKVKTRVV-HDTVN 519
           PS   K+     G+LSV VI A  L   D +G +DPYV L +   +   K  VV H  +N
Sbjct: 251 PSKAMKKPV---GLLSVKVIKAIKLKKKDLLGGSDPYVKLTLSGDKVPGKKTVVKHSNLN 310

Query: 520 PVWNQTFDFLVEDALHDMLIVEVWDHDTFGK-DKLGRVIMTL 560
           P WN+ FD +V++     L + V+D +  GK DK+G  ++ L
Sbjct: 311 PEWNEEFDLVVKEPESQELQLIVYDWEQVGKHDKIGMNVIQL 349

BLAST of Cucsa.310180 vs. TrEMBL
Match: A0A0A0LDN3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G876030 PE=4 SV=1)

HSP 1 Score: 1115.5 bits (2884), Expect = 0.0e+00
Identity = 569/597 (95.31%), Postives = 569/597 (95.31%), Query Frame = 1

Query: 1   MSFFSGIFLGVVVGVLLVIAFARAGNARAKHRSDLATTIAAFARMTAQDSRKILPKEFYP 60
           MSFFSGIFLGVVVGVLLVIAFARAGNARAKHRSDLATTIAAFARMTAQDSRKILPKEFYP
Sbjct: 1   MSFFSGIFLGVVVGVLLVIAFARAGNARAKHRSDLATTIAAFARMTAQDSRKILPKEFYP 60

Query: 61  SWVVFTQRQKLTWLNLQLDKIWPYVDAAASELIRSNVEPVLEEFRPVILSSLKFSKLTLG 120
           SWVVFTQRQKLTWLNLQLDKIWPYVDAAASELIRSNVEPVLEEFRPVILSSLKFSKLTLG
Sbjct: 61  SWVVFTQRQKLTWLNLQLDKIWPYVDAAASELIRSNVEPVLEEFRPVILSSLKFSKLTLG 120

Query: 121 TVAPSFTGISVLEDEPDTGGITLELEMQWDGNPNIVLDIKTKLGVSLPVQVKDIAFTGLF 180
           TVAPSFTGISVLEDEPDTGGITLELEMQWDGNPNIVLDIKTKLGVSLPVQVKDIAFTGLF
Sbjct: 121 TVAPSFTGISVLEDEPDTGGITLELEMQWDGNPNIVLDIKTKLGVSLPVQVKDIAFTGLF 180

Query: 181 RLIFKPLVDEFPCFGAVCYSLRKKKNLDFKLKIIGGDISSIPGVSDAIEETIRDAIEGTI 240
           RLIFKPLVDEFPCFGAVCYSLRKKKNLDFKLKIIGGDISSIPGVSDAIEETIRDAIEGTI
Sbjct: 181 RLIFKPLVDEFPCFGAVCYSLRKKKNLDFKLKIIGGDISSIPGVSDAIEETIRDAIEGTI 240

Query: 241 TWPVRKIVPILAGDYSDLEVKPVGTLEVKLVQAKELTNKDIIGKSDPYAVLFVRPLKERM 300
           TWPVRKIVPILAGDYSDLEVKPVGTLEVKLVQAKELTNKDIIGKSDPYAVLFVRPLKERM
Sbjct: 241 TWPVRKIVPILAGDYSDLEVKPVGTLEVKLVQAKELTNKDIIGKSDPYAVLFVRPLKERM 300

Query: 301 KTSKTINNQLNPIWNEHFNFIVEDASTQHLTIRVFDDEGVQASELIGCAQVALKDLEPGK 360
           KTSKTINNQLNPIWNEHFNFIVEDASTQHLTIRVFDDEGVQASELIGCAQVALKDLEPGK
Sbjct: 301 KTSKTINNQLNPIWNEHFNFIVEDASTQHLTIRVFDDEGVQASELIGCAQVALKDLEPGK 360

Query: 361 VKDVWLKLVKDLEIQRDNKYRGQVRNPLHRFQLNLGCIKQSNLLNFYACTQVHLELLYYP 420
           VKDVWLKLVKDLEIQRDNKYRG                            QVHLELLYYP
Sbjct: 361 VKDVWLKLVKDLEIQRDNKYRG----------------------------QVHLELLYYP 420

Query: 421 YGTDQSLYINPFNPDYALTSVEKALKMAPSGSEDADSGKPSSPKKRDTIVRGVLSVTVIA 480
           YGTDQSLYINPFNPDYALTSVEKALKMAPSGSEDADSGKPSSPKKRDTIVRGVLSVTVIA
Sbjct: 421 YGTDQSLYINPFNPDYALTSVEKALKMAPSGSEDADSGKPSSPKKRDTIVRGVLSVTVIA 480

Query: 481 AEDLPAVDFMGKADPYVVLIMKKSETKVKTRVVHDTVNPVWNQTFDFLVEDALHDMLIVE 540
           AEDLPAVDFMGKADPYVVLIMKKSETKVKTRVVHDTVNPVWNQTFDFLVEDALHDMLIVE
Sbjct: 481 AEDLPAVDFMGKADPYVVLIMKKSETKVKTRVVHDTVNPVWNQTFDFLVEDALHDMLIVE 540

Query: 541 VWDHDTFGKDKLGRVIMTLTRAILEGEIQDNFPLEGAKSGRVFLHLKWAAQPMFRDT 598
           VWDHDTFGKDKLGRVIMTLTRAILEGEIQDNFPLEGAKSGRVFLHLKWAAQPMFRDT
Sbjct: 541 VWDHDTFGKDKLGRVIMTLTRAILEGEIQDNFPLEGAKSGRVFLHLKWAAQPMFRDT 569

BLAST of Cucsa.310180 vs. TrEMBL
Match: D7SUW8_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_04s0008g05740 PE=4 SV=1)

HSP 1 Score: 866.3 bits (2237), Expect = 2.2e-248
Identity = 433/604 (71.69%), Postives = 504/604 (83.44%), Query Frame = 1

Query: 1   MSFFSGIFLGVVVGVLLVIAFARAGNARAKHRSDLATTIAAFARMTAQDSRKILPKEFYP 60
           MSFF GI +G+  G+ +V+ FAR  N R+K R DLATT+AAFARMT QDSRKILP +FYP
Sbjct: 1   MSFFFGIVIGIACGIAVVVVFARLANLRSKSRCDLATTVAAFARMTVQDSRKILPPQFYP 60

Query: 61  SWVVFTQRQKL-------TWLNLQLDKIWPYVDAAASELIRSNVEPVLEEFRPVILSSLK 120
           SWVVF+Q+QKL       TWLN  L KIWPYVD AASELIRSNVEP+LE++RPVILS+LK
Sbjct: 61  SWVVFSQQQKLSYMNGCLTWLNSHLRKIWPYVDEAASELIRSNVEPILEQYRPVILSALK 120

Query: 121 FSKLTLGTVAPSFTGISVLEDEPDTGGITLELEMQWDGNPNIVLDIKTKLGVSLPVQVKD 180
           FSKLTLGTVAP FTG+S+LE E   G +++ELEMQWDGNPNIVLDIKT++GV LPVQVK+
Sbjct: 121 FSKLTLGTVAPQFTGVSILEGE--AGEVSMELEMQWDGNPNIVLDIKTRVGVGLPVQVKN 180

Query: 181 IAFTGLFRLIFKPLVDEFPCFGAVCYSLRKKKNLDFKLKIIGGDISSIPGVSDAIEETIR 240
           I FTG+FRLIFKP+V+EFPCFGAVCYSLR+KKNLDFKLK++GGDI+++PG+SDAIEETI 
Sbjct: 181 IGFTGVFRLIFKPMVEEFPCFGAVCYSLREKKNLDFKLKVVGGDITALPGISDAIEETIL 240

Query: 241 DAIEGTITWPVRKIVPILAGDYSDLEVKPVGTLEVKLVQAKELTNKDIIGKSDPYAVLFV 300
           DAIE +ITWPVRKIVPI+ GDYSDLE+KPVGTLEVKLVQA++LTNKD+IGKSDPYAVLFV
Sbjct: 241 DAIEDSITWPVRKIVPIIPGDYSDLELKPVGTLEVKLVQARDLTNKDLIGKSDPYAVLFV 300

Query: 301 RPLKERMKTSKTINNQLNPIWNEHFNFIVEDASTQHLTIRVFDDEGVQASELIGCAQVAL 360
           RP+++RMKTSKTINN+LNPIWNEHF FIVEDASTQHLT+R+FDDEGVQASELIGCAQV L
Sbjct: 301 RPIRDRMKTSKTINNELNPIWNEHFEFIVEDASTQHLTVRIFDDEGVQASELIGCAQVRL 360

Query: 361 KDLEPGKVKDVWLKLVKDLEIQRDNKYRGQVRNPLHRFQLNLGCIKQSNLLNFYACTQVH 420
           KDLEPGKVKDVWLKLVKDL++QRD KYRG+VR                            
Sbjct: 361 KDLEPGKVKDVWLKLVKDLDVQRDKKYRGEVR---------------------------- 420

Query: 421 LELLYYPYGTDQSLYINPFNPDYALTSVEKALKMAPSGSEDADSGKPSSPKKRDTIVRGV 480
           LELLY P+G  +S++ NPF P+  LTS+EK LK    G+E  D  K  S KKRD IVRGV
Sbjct: 421 LELLYCPFGM-ESVFTNPFRPN-LLTSLEKVLK--ADGTEADDIKKSHSLKKRDIIVRGV 480

Query: 481 LSVTVIAAEDLPAVDFMGKADPYVVLIMKKSETKVKTRVVHDTVNPVWNQTFDFLVEDAL 540
           LSVTVI+AE+LP VD +GKADPYV L MKKS TK +TRVV++++NP+WNQTFDF+VED L
Sbjct: 481 LSVTVISAENLPVVDLIGKADPYVELTMKKSNTKHRTRVVNNSLNPIWNQTFDFVVEDGL 540

Query: 541 HDMLIVEVWDHDTFGKDKLGRVIMTLTRAILEGEIQDNFPLEGAKSGRVFLHLKWAAQPM 598
           HDMLI++VWDHDTFGKDK+GR I TLTR ILEGE +DNFPLEGAKSG + LHLKW+ QP+
Sbjct: 541 HDMLILDVWDHDTFGKDKIGRCIFTLTRVILEGEFRDNFPLEGAKSGSLNLHLKWSPQPI 570

BLAST of Cucsa.310180 vs. TrEMBL
Match: A0A061GJ38_THECC (Calcium-dependent lipid-binding (CaLB domain) family protein OS=Theobroma cacao GN=TCM_036969 PE=4 SV=1)

HSP 1 Score: 859.8 bits (2220), Expect = 2.0e-246
Identity = 423/596 (70.97%), Postives = 504/596 (84.56%), Query Frame = 1

Query: 1   MSFFSGIFLGVVVGVLLVIAFARAGNARAKHRSDLATTIAAFARMTAQDSRKILPKEFYP 60
           MSFF G+ +G+ +G+ L++AFAR    R+  RS LA T+A+FARMT QDSRKILP +FYP
Sbjct: 1   MSFFFGMLVGLTIGIGLIVAFARFEKIRSSRRSFLAKTVASFARMTVQDSRKILPPQFYP 60

Query: 61  SWVVFTQRQKLTWLNLQLDKIWPYVDAAASELIRSNVEPVLEEFRPVILSSLKFSKLTLG 120
            WVVF+Q QKL WLNLQL KIWP VD AASELIR++VEP+LE++ P ILSS+KF+KLTLG
Sbjct: 61  PWVVFSQHQKLVWLNLQLKKIWPSVDQAASELIRASVEPILEQYTPAILSSMKFAKLTLG 120

Query: 121 TVAPSFTGISVLEDEPDTGGITLELEMQWDGNPNIVLDIKTKLGVSLPVQVKDIAFTGLF 180
           TVAP FTG+S++E+E   GGIT+ELE+QWDGNPNIVL+I T+LGV+LPVQVK+I FTG+F
Sbjct: 121 TVAPQFTGVSIIENESGDGGITMELELQWDGNPNIVLNINTRLGVALPVQVKNIGFTGVF 180

Query: 181 RLIFKPLVDEFPCFGAVCYSLRKKKNLDFKLKIIGGDISSIPGVSDAIEETIRDAIEGTI 240
           RLIFKPLVD+FPCFGAV YSLR+KK+L F LK++GGDIS+IPG+SDAIE+TIR+AIE +I
Sbjct: 181 RLIFKPLVDQFPCFGAVSYSLREKKDLSFTLKVVGGDISTIPGISDAIEDTIRNAIEDSI 240

Query: 241 TWPVRKIVPILAGDYSDLEVKPVGTLEVKLVQAKELTNKDIIGKSDPYAVLFVRPLKERM 300
           TWPVRKIVPIL GDYS LE+KPVGTL+VKLVQAK+LTNKDI+GKSDP+AVLF+RPL+++M
Sbjct: 241 TWPVRKIVPILPGDYSFLELKPVGTLDVKLVQAKDLTNKDIVGKSDPFAVLFIRPLRDKM 300

Query: 301 KTSKTINNQLNPIWNEHFNFIVEDASTQHLTIRVFDDEGVQASELIGCAQVALKDLEPGK 360
           KTSKTINNQLNP+WNEHF FIVEDASTQHLTI+V+DDEGVQA+ELIGCAQVALKDLEPGK
Sbjct: 301 KTSKTINNQLNPVWNEHFEFIVEDASTQHLTIKVYDDEGVQAAELIGCAQVALKDLEPGK 360

Query: 361 VKDVWLKLVKDLEIQRDNKYRGQVRNPLHRFQLNLGCIKQSNLLNFYACTQVHLELLYYP 420
           VKD+WLKLVKDL +QRD KYRG                            QVH+ELLY P
Sbjct: 361 VKDIWLKLVKDLVVQRDTKYRG----------------------------QVHIELLYCP 420

Query: 421 YGTDQSLYINPFNPDYALTSVEKALKMAPSGSEDADSGKPSSPKKRDTIVRGVLSVTVIA 480
           +GT+ S + NPFNPD++LTS+EKALK   +  E AD  +  + +KRD IVRGVLSVTVIA
Sbjct: 421 FGTESS-FKNPFNPDFSLTSLEKALKNV-TVEEAADLKRTMTQRKRDVIVRGVLSVTVIA 480

Query: 481 AEDLPAVDFMGKADPYVVLIMKKSETKVKTRVVHDTVNPVWNQTFDFLVEDALHDMLIVE 540
           AE+LPAVDFMGKADP+VVLIMKK+ETK KTRV  +T+NPVWNQTFDF+VEDALH+MLI+E
Sbjct: 481 AENLPAVDFMGKADPFVVLIMKKAETKAKTRVATETLNPVWNQTFDFVVEDALHEMLILE 540

Query: 541 VWDHDTFGKDKLGRVIMTLTRAILEGEIQDNFPLEGAKSGRVFLHLKWAAQPMFRD 597
           VWDHDTF KDK+GR IMTLTR +LEGE+QD F L+GAKSG++FLHLKW  Q +FRD
Sbjct: 541 VWDHDTFKKDKIGRCIMTLTRVLLEGELQDGFLLDGAKSGKLFLHLKWNPQLVFRD 566

BLAST of Cucsa.310180 vs. TrEMBL
Match: A0A067KEY0_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_07139 PE=4 SV=1)

HSP 1 Score: 857.8 bits (2215), Expect = 7.7e-246
Identity = 426/606 (70.30%), Postives = 504/606 (83.17%), Query Frame = 1

Query: 1   MSFFSGIFLGVVVGVLLVIAFARAGNARAKHRSDLATTIAAFARMTAQDSRKILPKEFYP 60
           M FF+G+ +G+ +G+ L++ FAR  N R++ RS+LA T+AAFARMT QDSRK+LP EFYP
Sbjct: 1   MGFFTGMMIGMAIGIGLILGFARYQNVRSQRRSELAKTVAAFARMTVQDSRKLLPPEFYP 60

Query: 61  SWVVFTQRQKL--------TWLNLQLDKIWPYVDAAASELIRSNVEPVLEEFRPVILSSL 120
           SWVVF+QRQKL        TWLNLQL+KIWPYVD AASELIRSNVEP+LE++RP IL+SL
Sbjct: 61  SWVVFSQRQKLSLNRKGNLTWLNLQLNKIWPYVDEAASELIRSNVEPILEQYRPAILASL 120

Query: 121 KFSKLTLGTVAPSFTGISVLEDEPDTGGITLELEMQWDGNPNIVLDIKTKLGVSLPVQVK 180
           KFSKLTLGTVAP FTGI++LE+  + G +T+E+EMQWDGNPNIVLDI T++GV+LP+QVK
Sbjct: 121 KFSKLTLGTVAPQFTGITILEEGCEPGSVTMEIEMQWDGNPNIVLDINTRVGVALPIQVK 180

Query: 181 DIAFTGLFRLIFKPLVDEFPCFGAVCYSLRKKKNLDFKLKIIGGDISSIPGVSDAIEETI 240
           +I FTG+FRLIFKPLV+E P FGAV YSLR+KK LDF LK+IGGD+S+IPG+SDAIEETI
Sbjct: 181 NIGFTGVFRLIFKPLVEEIPGFGAVSYSLREKKKLDFTLKVIGGDLSAIPGISDAIEETI 240

Query: 241 RDAIEGTITWPVRKIVPILAGDYSDLEVKPVGTLEVKLVQAKELTNKDIIGKSDPYAVLF 300
           RDAIE +ITWPVRKIV IL GDYSDLE KPVGTLEVKL+Q KELTNKD+IGKSDP+AV+F
Sbjct: 241 RDAIEDSITWPVRKIVSILPGDYSDLEAKPVGTLEVKLIQGKELTNKDVIGKSDPFAVVF 300

Query: 301 VRPLKERMKTSKTINNQLNPIWNEHFNFIVEDASTQHLTIRVFDDEGVQASELIGCAQVA 360
           +RPL++RMKTS+TINN LNPIWNEHF F+VED  TQ LT+RVFDDEGVQA+ELIGCAQVA
Sbjct: 301 IRPLRDRMKTSRTINNNLNPIWNEHFEFMVEDPLTQRLTVRVFDDEGVQAAELIGCAQVA 360

Query: 361 LKDLEPGKVKDVWLKLVKDLEIQRDNKYRGQVRNPLHRFQLNLGCIKQSNLLNFYACTQV 420
           LKDLEPGKVK+VWLKLVKDLE+QRD KYRG                            QV
Sbjct: 361 LKDLEPGKVKEVWLKLVKDLEVQRDTKYRG----------------------------QV 420

Query: 421 HLELLYYPYGTDQSLYINPFNPDYALTSVEKALKMAPSGSEDADSG--KPSSPKKRDTIV 480
           HLELLY P+GT+ +L  NPFNPD+ LT++EKA K     +E+ +    K S+ +K++ IV
Sbjct: 421 HLELLYCPFGTESNLK-NPFNPDFQLTTLEKACK-----TEETEEAILKTSASQKKNVIV 480

Query: 481 RGVLSVTVIAAEDLPAVDFMGKADPYVVLIMKKSETKVKTRVVHDTVNPVWNQTFDFLVE 540
           RGVLSV V+AAE+LPAVD MGKADPYVVL MKKS+TKVKTRVV++++NPVWNQTFDF+VE
Sbjct: 481 RGVLSVNVVAAENLPAVDLMGKADPYVVLQMKKSDTKVKTRVVNESLNPVWNQTFDFVVE 540

Query: 541 DALHDMLIVEVWDHDTFGKDKLGRVIMTLTRAILEGEIQDNFPLEGAKSGRVFLHLKWAA 597
           DALHDMLI+EVWDHDTFGKDK+GR IMTLTR ILEGE QD+FPLEGAKSG++FLHLKW  
Sbjct: 541 DALHDMLILEVWDHDTFGKDKIGRCIMTLTRVILEGEFQDSFPLEGAKSGKLFLHLKWTP 572

BLAST of Cucsa.310180 vs. TrEMBL
Match: A0A0R0JHJ4_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_06G066600 PE=4 SV=1)

HSP 1 Score: 840.1 bits (2169), Expect = 1.7e-240
Identity = 408/605 (67.44%), Postives = 505/605 (83.47%), Query Frame = 1

Query: 1   MSFFSGIFLGVVVGVLLVIAFARAGNARAKHRSDLATTIAAFARMTAQDSRKILPKEFYP 60
           M F SG+ +G+ +G++L++AFAR G+ R+K RSDLA TIA FARMT +DSRKILP +FYP
Sbjct: 1   MGFISGMIVGIAIGIVLIVAFARQGSVRSKRRSDLAKTIAQFARMTVEDSRKILPPKFYP 60

Query: 61  SWVVFTQRQKLT--------WLNLQLDKIWPYVDAAASELIRSNVEPVLEEFRPVILSSL 120
           SWVVFT+RQKL+        WLN Q++KIWP+V+ AASELIR+NVEP+LE++RP+ILSSL
Sbjct: 61  SWVVFTRRQKLSSYIHFLLSWLNSQVEKIWPFVNEAASELIRTNVEPILEQYRPIILSSL 120

Query: 121 KFSKLTLGTVAPSFTGISVLEDEPDTGGITLELEMQWDGNPNIVLDIKTKLGVSLPVQVK 180
            FSKLTLGTVAP FTG++++E+     G+T++LEMQWDGNPNIVLDIKT++GV LPVQVK
Sbjct: 121 TFSKLTLGTVAPQFTGVTIVEENSGPEGVTMDLEMQWDGNPNIVLDIKTRVGVVLPVQVK 180

Query: 181 DIAFTGLFRLIFKPLVDEFPCFGAVCYSLRKKKNLDFKLKIIGGDISSIPGVSDAIEETI 240
           +I FTG+FRLIFKPLVDEFP FGAVC+SL++KK+LDF LK+IGGD+S++PG+SDAIEETI
Sbjct: 181 NIGFTGVFRLIFKPLVDEFPAFGAVCFSLKEKKDLDFTLKVIGGDLSTLPGISDAIEETI 240

Query: 241 RDAIEGTITWPVRKIVPILAGDYSDLEVKPVGTLEVKLVQAKELTNKDIIGKSDPYAVLF 300
           RDAIE +ITWPVRK++PIL GDYS+LE+KPVG LEVKLVQAK LTNKDI+GKSDPYAV+F
Sbjct: 241 RDAIEDSITWPVRKVIPILPGDYSNLELKPVGKLEVKLVQAKNLTNKDIVGKSDPYAVIF 300

Query: 301 VRPLKERMKTSKTINNQLNPIWNEHFNFIVEDASTQHLTIRVFDDEGVQASELIGCAQVA 360
           VRPL++R KTSK +NNQLNP+WNEHF FI+EDASTQHLT+R+FDDEGVQASELIGCAQV+
Sbjct: 301 VRPLRDRTKTSKIMNNQLNPVWNEHFEFIIEDASTQHLTVRIFDDEGVQASELIGCAQVS 360

Query: 361 LKDLEPGKVKDVWLKLVKDLEIQRDNKYRGQVRNPLHRFQLNLGCIKQSNLLNFYACTQV 420
           LKDLEPGKVKDVWLKLVKDLE+ RDNKYRG                            +V
Sbjct: 361 LKDLEPGKVKDVWLKLVKDLEVHRDNKYRG----------------------------EV 420

Query: 421 HLELLYYPYGTDQSLYINPFNPDYALTSVEKALKMAPSGSEDADS-GKPSSPKKRDTIVR 480
           HLELLY P+G + ++  NPF+PD++LT+ EK LK   SG+ DA++     S ++ + IVR
Sbjct: 421 HLELLYCPFGVESAIR-NPFDPDFSLTTFEKTLK---SGTGDAEAEDLIGSRRRNNVIVR 480

Query: 481 GVLSVTVIAAEDLPAVDFMGKADPYVVLIMKKSETKVKTRVVHDTVNPVWNQTFDFLVED 540
           GVLSVTVI+AEDLPAVD MGKADP+VVL++KK+E K+KTRVV++++NPVWNQTFDF+VED
Sbjct: 481 GVLSVTVISAEDLPAVDLMGKADPFVVLLLKKTEKKLKTRVVNESLNPVWNQTFDFVVED 540

Query: 541 ALHDMLIVEVWDHDTFGKDKLGRVIMTLTRAILEGEIQDNFPLEGAKSGRVFLHLKWAAQ 597
            LH+MLI+EV+DHDTFGK+K+GRVI+TLT+ ILEGE  + +PL+GAKSG + LHLKW  Q
Sbjct: 541 GLHEMLILEVYDHDTFGKEKIGRVILTLTKVILEGEYNETYPLDGAKSGNISLHLKWTPQ 573

BLAST of Cucsa.310180 vs. TAIR10
Match: AT5G11100.1 (AT5G11100.1 Calcium-dependent lipid-binding (CaLB domain) family protein)

HSP 1 Score: 818.1 bits (2112), Expect = 3.4e-237
Identity = 405/596 (67.95%), Postives = 481/596 (80.70%), Query Frame = 1

Query: 1   MSFFSGIFLGVVVGVLLVIAFARAGNARAKHRSDLATTIAAFARMTAQDSRKILPKEFYP 60
           M F  G+F+G+ V   LV+AFAR  + R+  R+DLA TIAAFARMT QDSRK+LP +FYP
Sbjct: 1   MGFLFGLFIGIAVSFGLVVAFARYSSVRSTRRADLAKTIAAFARMTVQDSRKLLPGDFYP 60

Query: 61  SWVVFTQRQKLTWLNLQLDKIWPYVDAAASELIRSNVEPVLEEFRPVILSSLKFSKLTLG 120
           SWVVF+QRQKL WLNL+L+KIWPYV+ AASELI+S+VEPVLE++ P +L+SLKFSK TLG
Sbjct: 61  SWVVFSQRQKLNWLNLELEKIWPYVNEAASELIKSSVEPVLEQYTPAMLASLKFSKFTLG 120

Query: 121 TVAPSFTGISVLEDEPDTGGITLELEMQWDGNPNIVLDIKTKLGVSLPVQVKDIAFTGLF 180
           TVAP FTG+S+LE E    GIT+ELEMQWDGNP IVLD+KT LGVSLP++VK+I FTG+F
Sbjct: 121 TVAPQFTGVSILESESGPNGITMELEMQWDGNPKIVLDVKTLLGVSLPIEVKNIGFTGVF 180

Query: 181 RLIFKPLVDEFPCFGAVCYSLRKKKNLDFKLKIIGGDISSIPGVSDAIEETIRDAIEGTI 240
           RLIFKPLVDEFPCFGA+ YSLR+KK LDF LK+IGG+++SIPG+SDAIEETIRDAIE +I
Sbjct: 181 RLIFKPLVDEFPCFGALSYSLREKKGLDFTLKVIGGELTSIPGISDAIEETIRDAIEDSI 240

Query: 241 TWPVRKIVPILAGDYSDLEVKPVGTLEVKLVQAKELTNKDIIGKSDPYAVLFVRPLKERM 300
           TWPVRKI+PIL GDYSDLE+KPVG L+VK+VQAK+L NKD+IGKSDPYA++F+RPL +R 
Sbjct: 241 TWPVRKIIPILPGDYSDLELKPVGKLDVKVVQAKDLANKDMIGKSDPYAIVFIRPLPDRT 300

Query: 301 KTSKTINNQLNPIWNEHFNFIVEDASTQHLTIRVFDDEGVQASELIGCAQVALKDLEPGK 360
           K +KTI+N LNPIWNEHF FIVED STQHLT+RVFDDEGV +S+LIG AQV L +L PGK
Sbjct: 301 KKTKTISNSLNPIWNEHFEFIVEDVSTQHLTVRVFDDEGVGSSQLIGAAQVPLNELVPGK 360

Query: 361 VKDVWLKLVKDLEIQRDNKYRGQVRNPLHRFQLNLGCIKQSNLLNFYACTQVHLELLYYP 420
           VKD+WLKLVKDLEIQRD K RGQV+                            LELLY P
Sbjct: 361 VKDIWLKLVKDLEIQRDTKNRGQVQ----------------------------LELLYCP 420

Query: 421 YGTDQSLYINPFNPDYALTSVEKALKMAPSGSEDADSGKPSSPKKRDTIVRGVLSVTVIA 480
            G +  L  NPFNPDY+LT +EK LK     S+  D  K  + KK+D IVRGVLSVTV+A
Sbjct: 421 LGKEGGLK-NPFNPDYSLTILEKVLKPESEDSDATDMKKLVTSKKKDVIVRGVLSVTVVA 480

Query: 481 AEDLPAVDFMGKADPYVVLIMKKSETKVKTRVVHDTVNPVWNQTFDFLVEDALHDMLIVE 540
           AEDLPAVDFMGKAD +VV+ +KKSETK KTRVV D++NPVWNQTFDF+VEDALHD+L +E
Sbjct: 481 AEDLPAVDFMGKADAFVVITLKKSETKSKTRVVPDSLNPVWNQTFDFVVEDALHDLLTLE 540

Query: 541 VWDHDTFGKDKLGRVIMTLTRAILEGEIQDNFPLEGAKSGRVFLHLKWAAQPMFRD 597
           VWDHD FGKDK+GRVIMTLTR +LEGE Q+ F L+GAKSG++ +HLKW  +   RD
Sbjct: 541 VWDHDKFGKDKIGRVIMTLTRVMLEGEFQEWFELDGAKSGKLCVHLKWTPRLKLRD 567

BLAST of Cucsa.310180 vs. TAIR10
Match: AT1G05500.1 (AT1G05500.1 Calcium-dependent lipid-binding (CaLB domain) family protein)

HSP 1 Score: 793.1 bits (2047), Expect = 1.2e-229
Identity = 388/597 (64.99%), Postives = 482/597 (80.74%), Query Frame = 1

Query: 1   MSFFSGIFLGVVVGVLLVIAFARAGNARAKHRSDLATTIAAFARMTAQDSRKILPKEFYP 60
           M F  G+ +G++VG+ ++I F +  N+R+K RS+LA T+AAFARMT +DSRK+LP EFYP
Sbjct: 1   MGFIVGVVIGLLVGIAIIIGFVKLENSRSKLRSELANTVAAFARMTVEDSRKLLPPEFYP 60

Query: 61  SWVVFTQRQKLTWLNLQLDKIWPYVDAAASELIRSNVEPVLEEFRPVILSSLKFSKLTLG 120
           SWVVF++RQKLTWLN  L KIWPYVD AASELI+++VEPVLE++RP I++SL FSKLTLG
Sbjct: 61  SWVVFSERQKLTWLNHHLTKIWPYVDEAASELIKASVEPVLEQYRPAIVASLTFSKLTLG 120

Query: 121 TVAPSFTGISVLEDEPDTGGITLELEMQWDGNPNIVLDIKTKLGVSLPVQVKDIAFTGLF 180
           TVAP FTG+SV++   D  GITLEL+MQWDGNPNIVL +KT +GVSLP+QVK+I FTG+F
Sbjct: 121 TVAPQFTGVSVIDG--DKNGITLELDMQWDGNPNIVLGVKTLVGVSLPIQVKNIGFTGVF 180

Query: 181 RLIFKPLVDEFPCFGAVCYSLRKKKNLDFKLKIIGGDISSIPGVSDAIEETIRDAIEGTI 240
           RLIF+PLV++FPCFGAV  SLR+KK LDF LK++GGDIS+IPG+S+AIEETIRDA+E +I
Sbjct: 181 RLIFRPLVEDFPCFGAVSVSLREKKKLDFTLKVVGGDISAIPGLSEAIEETIRDAVEDSI 240

Query: 241 TWPVRKIVPILAGDYSDLEVKPVGTLEVKLVQAKELTNKDIIGKSDPYAVLFVRPLKERM 300
           TWPVRK++PI+ GDYSDLE+KPVG LEVKLVQAK LTNKD++GKSDP+A +F+RPL+E+ 
Sbjct: 241 TWPVRKVIPIIPGDYSDLELKPVGMLEVKLVQAKNLTNKDLVGKSDPFAKMFIRPLREKT 300

Query: 301 KTSKTINNQLNPIWNEHFNFIVEDASTQHLTIRVFDDEGVQASELIGCAQVALKDLEPGK 360
           K SKTINN LNPIWNEHF F+VEDASTQHL +R++DDEGVQASELIGCAQ+ L +LEPGK
Sbjct: 301 KRSKTINNDLNPIWNEHFEFVVEDASTQHLVVRIYDDEGVQASELIGCAQIRLCELEPGK 360

Query: 361 VKDVWLKLVKDLEIQRDNKYRGQVRNPLHRFQLNLGCIKQSNLLNFYACTQVHLELLYYP 420
           VKDVWLKLVKDLEIQRD K RG                            +VHLELLY P
Sbjct: 361 VKDVWLKLVKDLEIQRDTKNRG----------------------------EVHLELLYIP 420

Query: 421 YGTDQSLYINPFNPDYALTSVEKALKMAPSGSEDADSGKPSSPKKRDTIVRGVLSVTVIA 480
           YG+   + +NPF    ++TS+E+ LK     ++  D    SS K++D IVRGVLSVTVI+
Sbjct: 421 YGSGNGI-VNPFVTS-SMTSLERVLK-----NDTTDEENASSRKRKDVIVRGVLSVTVIS 480

Query: 481 AEDLPAVDFMGKADPYVVLIMKKSETKVKTRVVHDTVNPVWNQTFDFLVEDALHDMLIVE 540
           AE++P  D MGKADPYVVL MKKS  K KTRVV+D++NPVWNQTFDF+VED LHDML++E
Sbjct: 481 AEEIPIQDLMGKADPYVVLSMKKSGAKSKTRVVNDSLNPVWNQTFDFVVEDGLHDMLVLE 540

Query: 541 VWDHDTFGKDKLGRVIMTLTRAILEGEIQDNFPLEGAKSGRVFLHLKWAAQPMFRDT 598
           VWDHDTFGKD +GR I+TLTR I+E E +D +PL+ +K+G++ LHLKW AQ ++RD+
Sbjct: 541 VWDHDTFGKDYIGRCILTLTRVIMEEEYKDWYPLDESKTGKLQLHLKWMAQSIYRDS 560

BLAST of Cucsa.310180 vs. TAIR10
Match: AT3G61050.1 (AT3G61050.1 Calcium-dependent lipid-binding (CaLB domain) family protein)

HSP 1 Score: 291.6 bits (745), Expect = 1.1e-78
Identity = 162/400 (40.50%), Postives = 245/400 (61.25%), Query Frame = 1

Query: 1   MSFFSGIFLGVVVGVLLVIAFARAGNARAKHRSDLATTIAAFARMTAQDSRKILPKEFYP 60
           M   SGI  G++ GV L+  ++R    R+  R   A  +     ++  D +KI    F P
Sbjct: 1   MGLISGILFGIIFGVALMAGWSRMMTHRSSKRVAKAVDMKLLGSLSRDDLKKICGDNF-P 60

Query: 61  SWVVFTQRQKLTWLNLQLDKIWPYVDAAASELIRSNVEPVLEEFRPVILSSLKFSKLTLG 120
            W+ F   +++ WLN  L K+WPY+  AA+ +IR +VEP+LE++RP  ++SLKFSKLTLG
Sbjct: 61  QWISFPAFEQVKWLNKLLSKMWPYIAEAATMVIRDSVEPLLEDYRPPGITSLKFSKLTLG 120

Query: 121 TVAPSFTGISVLEDEPDTGGITLELEMQWDGNPNIVLDIKTKLGVSLPVQVKDIAFTGLF 180
            VAP   GI V   +   G +T++++++W G+PNIVL + T L  S+P+Q+KD+    + 
Sbjct: 121 NVAPKIEGIRVQSFK--EGQVTMDVDLRWGGDPNIVLGV-TALVASIPIQLKDLQVFTVA 180

Query: 181 RLIFKPLVDEFPCFGAVCYSL--RKKKNLDFKLKIIGGDISSIPGVSDAIEETIRDAIEG 240
           R+IF+ L DE PC  AV  +L    K  +D+ LK +GG +++IPG+SD I++T+   ++ 
Sbjct: 181 RVIFQ-LADEIPCISAVVVALLAEPKPRIDYTLKAVGGSLTAIPGLSDMIDDTVDTIVKD 240

Query: 241 TITWPVRKIVPI--LAGDYSDLEVKPVGTLEVKLVQAKELTNKDIIGKSDPYAVLFVRPL 300
            + WP R +VPI  +  D SDLE+KP G L V +V+A  L NK++IGKSDPYA +++RP+
Sbjct: 241 MLQWPHRIVVPIGGIPVDLSDLELKPQGKLIVTVVKATNLKNKELIGKSDPYATIYIRPV 300

Query: 301 KERMKTSKTINNQLNPIWNEHFNFIVEDASTQHLTIRVFDDEGVQASELIGCAQVALKDL 360
             + KT K I N LNP+W++ F  I ED  TQ LT+ VFD + V   E +G  ++ L  L
Sbjct: 301 -FKYKT-KAIENNLNPVWDQTFELIAEDKETQSLTVEVFDKD-VGQDERLGLVKLPLSSL 360

Query: 361 EPGKVKDVWLKLVKDLEI--QRDNKYRGQVRNPLHRFQLN 395
           E G  K++ L L+  L+    +D K RG +   +H  + N
Sbjct: 361 EAGVTKELELNLLSSLDTLKVKDKKDRGSITLKVHYHEFN 392

HSP 2 Score: 67.0 bits (162), Expect = 4.5e-11
Identity = 34/90 (37.78%), Postives = 55/90 (61.11%), Query Frame = 1

Query: 471 RGVLSVTVIAAEDLPAVDFMGKADPYVVLIMKKSETKVKTRVVHDTVNPVWNQTFDFLVE 530
           +G L VTV+ A +L   + +GK+DPY  + ++    K KT+ + + +NPVW+QTF+ + E
Sbjct: 262 QGKLIVTVVKATNLKNKELIGKSDPYATIYIRPV-FKYKTKAIENNLNPVWDQTFELIAE 321

Query: 531 DALHDMLIVEVWDHDTFGKDKLGRVIMTLT 561
           D     L VEV+D D    ++LG V + L+
Sbjct: 322 DKETQSLTVEVFDKDVGQDERLGLVKLPLS 350

BLAST of Cucsa.310180 vs. TAIR10
Match: AT5G04220.2 (AT5G04220.2 Calcium-dependent lipid-binding (CaLB domain) family protein)

HSP 1 Score: 219.5 bits (558), Expect = 5.4e-57
Identity = 128/388 (32.99%), Postives = 211/388 (54.38%), Query Frame = 1

Query: 1   MSFFSGIFLGVVVGVLLVIAFARAGNARAKHRSDLATTIAAFARMTAQDSRKILPKEFYP 60
           + F  GI +G+++G  ++I    +       R  + T+I+            +LP    P
Sbjct: 11  IGFVIGIPIGLILGFFVLIYSQPSHQEYPPARPLVETSISVLL--------DLLPD--IP 70

Query: 61  SWVVFTQRQKLTWLNLQLDKIWPYVDAAASELIRSNVEPVLEEFRPVI-LSSLKFSKLTL 120
            W+     +++ W N  +  +WPY+D A   +IRS+V+P+  ++     + S++F  L+L
Sbjct: 71  LWMKNPDYERVDWFNKFISYMWPYLDKAVCGIIRSSVQPLFADYIGTFCIESIEFENLSL 130

Query: 121 GTVAPSFTGISVLEDEPDTGGITLELEMQWDGNPNIVLDIKTKLGVSLPVQVKDIAFTGL 180
           GT+ P+  G+   E       +  E  ++W GNPNIVL +K  L + + VQ+ D+ F  +
Sbjct: 131 GTLPPTVHGVKFYETNEKE--LLFEPSIKWAGNPNIVLVLKV-LSLRIRVQLVDLQFFAI 190

Query: 181 FRLIFKPLVDEFPCFGAVCYSLRKKKNLDFKLKIIGGDISSIPGVSDAIEETIRDAIEGT 240
            R+  KPL+  FPCFG V  SL +K ++DF LK++GGD+ SIPG+   ++ETI+  +   
Sbjct: 191 VRVALKPLLPTFPCFGMVVVSLMEKPHVDFGLKVLGGDLMSIPGLYRYVQETIKRQVSSM 250

Query: 241 ITWPVRKIVPILAGDYSDLEVKPVGTLEVKLVQAKELTNKDIIGKSDPYAVLFVRPLKER 300
             WP    +PIL    + ++ KPVG L V +++A+ L  KD++G SDPY  L +   K  
Sbjct: 251 YHWPQVLEIPILDSSTASVK-KPVGLLHVSILRARNLLKKDLLGTSDPYVKLSLTGEKLP 310

Query: 301 MKTSKTINNQLNPIWNEHFNFIVEDASTQHLTIRVFDDEGVQASELIGCAQVALKDLEPG 360
            K +      LNP WNEHF  IV+D ++Q L + VFD + V   + +G   + L+ + PG
Sbjct: 311 AKKTTIKKRNLNPEWNEHFKLIVKDPNSQVLQLEVFDWDKVGGHDRLGMQMIPLQKINPG 370

Query: 361 KVKDVWLKLVKDLEI---QRDNKYRGQV 385
           + K+  L L+K+  +     D K RG++
Sbjct: 371 ERKEFNLDLIKNSNVVMDSGDKKKRGRL 384

HSP 2 Score: 55.8 bits (133), Expect = 1.0e-07
Identity = 38/115 (33.04%), Postives = 63/115 (54.78%), Query Frame = 1

Query: 472 GVLSVTVIAAEDLPAVDFMGKADPYVVLIMKKSETKV-KTRVVHDTVNPVWNQTFDFLVE 531
           G+L V+++ A +L   D +G +DPYV L +   +    KT +    +NP WN+ F  +V+
Sbjct: 261 GLLHVSILRARNLLKKDLLGTSDPYVKLSLTGEKLPAKKTTIKKRNLNPEWNEHFKLIVK 320

Query: 532 DALHDMLIVEVWDHDTF-GKDKLGRVIMTLTRAILEGEIQDNFPLEGAKSGRVFL 585
           D    +L +EV+D D   G D+LG  ++ L + I  GE +  F L+  K+  V +
Sbjct: 321 DPNSQVLQLEVFDWDKVGGHDRLGMQMIPLQK-INPGE-RKEFNLDLIKNSNVVM 373

HSP 3 Score: 53.5 bits (127), Expect = 5.1e-07
Identity = 50/212 (23.58%), Postives = 95/212 (44.81%), Query Frame = 1

Query: 386 NPLHRFQLNLGCIKQSNLL----NFYACTQVHLELLYYPYGTDQSLYINPFNPDYALTSV 445
           NP  R + NL  IK SN++    +     ++ ++L Y P+                    
Sbjct: 354 NPGERKEFNLDLIKNSNVVMDSGDKKKRGRLEVDLRYVPFR------------------- 413

Query: 446 EKALKMAPSGSEDADSGKPSSPKKRDTIVRGVLSVTVIAAEDLPAVDFMGKADPYVVLIM 505
           E+++K      E+  S       +      G+LSV V +A+D+        ++PY V++ 
Sbjct: 414 EESIKRRKESREEKSSEDDDFLSQA-----GLLSVAVQSAKDVEGKK--KHSNPYAVVLF 473

Query: 506 KKSETKVKTRVVHDTVNPVWNQTFDFLVED-ALHDMLIVEVWDHDT----FGKDKLGRVI 565
           +    K KT+++  T +P WN+ F F +E+  + + + VEV    T      K++LG V 
Sbjct: 474 RGE--KKKTKMLKKTRDPRWNEEFQFTLEEPPVKESIRVEVMSKGTGFHFRSKEELGHVD 533

Query: 566 MTLTRAILEGEIQDNFPLEGAKSGRVFLHLKW 589
           + L   +  G I   + L  +++G + + ++W
Sbjct: 534 INLDDVVDNGRINQKYHLINSRNGIIHIEIRW 537

BLAST of Cucsa.310180 vs. TAIR10
Match: AT1G20080.1 (AT1G20080.1 Calcium-dependent lipid-binding (CaLB domain) family protein)

HSP 1 Score: 209.5 bits (532), Expect = 5.6e-54
Identity = 131/387 (33.85%), Postives = 204/387 (52.71%), Query Frame = 1

Query: 1   MSFFSGIFLGVVVGVLLVIAFARAGNARAKHRSDLATTIAAFARMTAQDSRKILPKEFYP 60
           + F  G  +G+V+G  L I F        +        I     + ++    + P+   P
Sbjct: 11  IGFGFGTTIGIVIGYYLFIYFQSTDVEDPE--------IKPLVELDSETIATMFPE--IP 70

Query: 61  SWVVFTQRQKLTWLNLQLDKIWPYVDAAASELIRSNVEPVLEEFRPVI-LSSLKFSKLTL 120
            WV      ++ WLN  +  +WPY+D A  ++ +S  +P++ E  P   + S++F  LTL
Sbjct: 71  MWVKNPDFDRIDWLNKLIGHMWPYMDKAICKMAKSIAKPIIAEQIPNYKIDSVEFEMLTL 130

Query: 121 GTVAPSFTGISVLEDEPDTGGITLELEMQWDGNPNIVLDIKTKLGVSLPVQVKDIAFTGL 180
           G++ PSF G+ V     D   I +EL ++W GNPNI++  K   G+   VQV D+     
Sbjct: 131 GSLPPSFQGMKVYAT--DDKEIIMELSVKWAGNPNIIVVAKA-FGLKATVQVIDLQVYAT 190

Query: 181 FRLIFKPLVDEFPCFGAVCYSLRKKKNLDFKLKIIGGDISSIPGVSDAIEETIRDAIEGT 240
            R+  KPLV  FPCF  +  SL  K  +DF LK++G D+ +IPG+   ++E I+D +   
Sbjct: 191 PRITLKPLVPSFPCFANIFVSLMDKPQVDFGLKLLGADVMAIPGLYRFVQEIIKDQVANM 250

Query: 241 ITWPVRKIVPILAGDYSDLEVKPVGTLEVKLVQAKELTNKDIIGKSDPYAVLFVRPLKER 300
             WP    V I+  D S    KPVG L VK+++A +L  KD++G SDPY  L +   K  
Sbjct: 251 YLWPKTLNVQIM--DPSKAMKKPVGLLSVKVIKAIKLKKKDLLGGSDPYVKLTLSGDKVP 310

Query: 301 MKTSKTINNQLNPIWNEHFNFIVEDASTQHLTIRVFDDEGVQASELIGCAQVALKDLEPG 360
            K +   ++ LNP WNE F+ +V++  +Q L + V+D E V   + IG   + LKDL P 
Sbjct: 311 GKKTVVKHSNLNPEWNEEFDLVVKEPESQELQLIVYDWEQVGKHDKIGMNVIQLKDLTPE 370

Query: 361 KVKDVWLKLVKDLEIQR--DNKYRGQV 385
           + K + L+L+K +E +     K RGQ+
Sbjct: 371 EPKLMTLELLKSMEPKEPVSEKSRGQL 382

HSP 2 Score: 58.9 bits (141), Expect = 1.2e-08
Identity = 38/102 (37.25%), Postives = 55/102 (53.92%), Query Frame = 1

Query: 460 PSSPKKRDTIVRGVLSVTVIAAEDLPAVDFMGKADPYVVLIMKKSETKVKTRVV-HDTVN 519
           PS   K+     G+LSV VI A  L   D +G +DPYV L +   +   K  VV H  +N
Sbjct: 251 PSKAMKKPV---GLLSVKVIKAIKLKKKDLLGGSDPYVKLTLSGDKVPGKKTVVKHSNLN 310

Query: 520 PVWNQTFDFLVEDALHDMLIVEVWDHDTFGK-DKLGRVIMTL 560
           P WN+ FD +V++     L + V+D +  GK DK+G  ++ L
Sbjct: 311 PEWNEEFDLVVKEPESQELQLIVYDWEQVGKHDKIGMNVIQL 349

BLAST of Cucsa.310180 vs. NCBI nr
Match: gi|778686896|ref|XP_004136361.2| (PREDICTED: synaptotagmin-4-like [Cucumis sativus])

HSP 1 Score: 1115.5 bits (2884), Expect = 0.0e+00
Identity = 569/597 (95.31%), Postives = 569/597 (95.31%), Query Frame = 1

Query: 1   MSFFSGIFLGVVVGVLLVIAFARAGNARAKHRSDLATTIAAFARMTAQDSRKILPKEFYP 60
           MSFFSGIFLGVVVGVLLVIAFARAGNARAKHRSDLATTIAAFARMTAQDSRKILPKEFYP
Sbjct: 1   MSFFSGIFLGVVVGVLLVIAFARAGNARAKHRSDLATTIAAFARMTAQDSRKILPKEFYP 60

Query: 61  SWVVFTQRQKLTWLNLQLDKIWPYVDAAASELIRSNVEPVLEEFRPVILSSLKFSKLTLG 120
           SWVVFTQRQKLTWLNLQLDKIWPYVDAAASELIRSNVEPVLEEFRPVILSSLKFSKLTLG
Sbjct: 61  SWVVFTQRQKLTWLNLQLDKIWPYVDAAASELIRSNVEPVLEEFRPVILSSLKFSKLTLG 120

Query: 121 TVAPSFTGISVLEDEPDTGGITLELEMQWDGNPNIVLDIKTKLGVSLPVQVKDIAFTGLF 180
           TVAPSFTGISVLEDEPDTGGITLELEMQWDGNPNIVLDIKTKLGVSLPVQVKDIAFTGLF
Sbjct: 121 TVAPSFTGISVLEDEPDTGGITLELEMQWDGNPNIVLDIKTKLGVSLPVQVKDIAFTGLF 180

Query: 181 RLIFKPLVDEFPCFGAVCYSLRKKKNLDFKLKIIGGDISSIPGVSDAIEETIRDAIEGTI 240
           RLIFKPLVDEFPCFGAVCYSLRKKKNLDFKLKIIGGDISSIPGVSDAIEETIRDAIEGTI
Sbjct: 181 RLIFKPLVDEFPCFGAVCYSLRKKKNLDFKLKIIGGDISSIPGVSDAIEETIRDAIEGTI 240

Query: 241 TWPVRKIVPILAGDYSDLEVKPVGTLEVKLVQAKELTNKDIIGKSDPYAVLFVRPLKERM 300
           TWPVRKIVPILAGDYSDLEVKPVGTLEVKLVQAKELTNKDIIGKSDPYAVLFVRPLKERM
Sbjct: 241 TWPVRKIVPILAGDYSDLEVKPVGTLEVKLVQAKELTNKDIIGKSDPYAVLFVRPLKERM 300

Query: 301 KTSKTINNQLNPIWNEHFNFIVEDASTQHLTIRVFDDEGVQASELIGCAQVALKDLEPGK 360
           KTSKTINNQLNPIWNEHFNFIVEDASTQHLTIRVFDDEGVQASELIGCAQVALKDLEPGK
Sbjct: 301 KTSKTINNQLNPIWNEHFNFIVEDASTQHLTIRVFDDEGVQASELIGCAQVALKDLEPGK 360

Query: 361 VKDVWLKLVKDLEIQRDNKYRGQVRNPLHRFQLNLGCIKQSNLLNFYACTQVHLELLYYP 420
           VKDVWLKLVKDLEIQRDNKYRG                            QVHLELLYYP
Sbjct: 361 VKDVWLKLVKDLEIQRDNKYRG----------------------------QVHLELLYYP 420

Query: 421 YGTDQSLYINPFNPDYALTSVEKALKMAPSGSEDADSGKPSSPKKRDTIVRGVLSVTVIA 480
           YGTDQSLYINPFNPDYALTSVEKALKMAPSGSEDADSGKPSSPKKRDTIVRGVLSVTVIA
Sbjct: 421 YGTDQSLYINPFNPDYALTSVEKALKMAPSGSEDADSGKPSSPKKRDTIVRGVLSVTVIA 480

Query: 481 AEDLPAVDFMGKADPYVVLIMKKSETKVKTRVVHDTVNPVWNQTFDFLVEDALHDMLIVE 540
           AEDLPAVDFMGKADPYVVLIMKKSETKVKTRVVHDTVNPVWNQTFDFLVEDALHDMLIVE
Sbjct: 481 AEDLPAVDFMGKADPYVVLIMKKSETKVKTRVVHDTVNPVWNQTFDFLVEDALHDMLIVE 540

Query: 541 VWDHDTFGKDKLGRVIMTLTRAILEGEIQDNFPLEGAKSGRVFLHLKWAAQPMFRDT 598
           VWDHDTFGKDKLGRVIMTLTRAILEGEIQDNFPLEGAKSGRVFLHLKWAAQPMFRDT
Sbjct: 541 VWDHDTFGKDKLGRVIMTLTRAILEGEIQDNFPLEGAKSGRVFLHLKWAAQPMFRDT 569

BLAST of Cucsa.310180 vs. NCBI nr
Match: gi|659132884|ref|XP_008466436.1| (PREDICTED: synaptotagmin-4-like [Cucumis melo])

HSP 1 Score: 1077.0 bits (2784), Expect = 0.0e+00
Identity = 543/597 (90.95%), Postives = 561/597 (93.97%), Query Frame = 1

Query: 1   MSFFSGIFLGVVVGVLLVIAFARAGNARAKHRSDLATTIAAFARMTAQDSRKILPKEFYP 60
           MSFFSGIF+GVVVGVLL+IA AR GN RAKHRSDLATTIAAFARMTAQDSRKILPKEFYP
Sbjct: 1   MSFFSGIFIGVVVGVLLIIALARVGNVRAKHRSDLATTIAAFARMTAQDSRKILPKEFYP 60

Query: 61  SWVVFTQRQKLTWLNLQLDKIWPYVDAAASELIRSNVEPVLEEFRPVILSSLKFSKLTLG 120
           SWVVFTQRQKLTWLNLQLDKIWPYVDAAASELIRSNVEPVLE+FRP+ILSSLKFSKLTLG
Sbjct: 61  SWVVFTQRQKLTWLNLQLDKIWPYVDAAASELIRSNVEPVLEQFRPMILSSLKFSKLTLG 120

Query: 121 TVAPSFTGISVLEDEPDTGGITLELEMQWDGNPNIVLDIKTKLGVSLPVQVKDIAFTGLF 180
           +VAP+ TGI+VLEDE DT GITLELEMQWDGNPNIVLDIKTKLGVSLPVQVKD+AFTGLF
Sbjct: 121 SVAPNLTGIAVLEDESDTNGITLELEMQWDGNPNIVLDIKTKLGVSLPVQVKDVAFTGLF 180

Query: 181 RLIFKPLVDEFPCFGAVCYSLRKKKNLDFKLKIIGGDISSIPGVSDAIEETIRDAIEGTI 240
           RL+FKPLVDEFPCFGAVCYSLRKKKNLDFKLKIIGGDISSIPGVSDAIEETIRDAIEGTI
Sbjct: 181 RLMFKPLVDEFPCFGAVCYSLRKKKNLDFKLKIIGGDISSIPGVSDAIEETIRDAIEGTI 240

Query: 241 TWPVRKIVPILAGDYSDLEVKPVGTLEVKLVQAKELTNKDIIGKSDPYAVLFVRPLKERM 300
           TWPVR+IVPILAGDYSDLEVKPVGTLEVKLVQAKELTNKDIIGKSDPYAVLFVRPLKERM
Sbjct: 241 TWPVRQIVPILAGDYSDLEVKPVGTLEVKLVQAKELTNKDIIGKSDPYAVLFVRPLKERM 300

Query: 301 KTSKTINNQLNPIWNEHFNFIVEDASTQHLTIRVFDDEGVQASELIGCAQVALKDLEPGK 360
           KTSKTINNQLNPIWNEHF+FIVEDASTQHLTIRVFDDEGVQASELIGCAQVALKDLEPGK
Sbjct: 301 KTSKTINNQLNPIWNEHFDFIVEDASTQHLTIRVFDDEGVQASELIGCAQVALKDLEPGK 360

Query: 361 VKDVWLKLVKDLEIQRDNKYRGQVRNPLHRFQLNLGCIKQSNLLNFYACTQVHLELLYYP 420
           VKDVWLKLVKDLEIQRDNKYRG                            QVHLELLYYP
Sbjct: 361 VKDVWLKLVKDLEIQRDNKYRG----------------------------QVHLELLYYP 420

Query: 421 YGTDQSLYINPFNPDYALTSVEKALKMAPSGSEDADSGKPSSPKKRDTIVRGVLSVTVIA 480
           +GTDQSLYINPFNPDYALTSVEKALKMAP+GSEDADSGK SSPKKRDTIVRGVLSVTVIA
Sbjct: 421 FGTDQSLYINPFNPDYALTSVEKALKMAPNGSEDADSGKQSSPKKRDTIVRGVLSVTVIA 480

Query: 481 AEDLPAVDFMGKADPYVVLIMKKSETKVKTRVVHDTVNPVWNQTFDFLVEDALHDMLIVE 540
           AEDLPAVDFMGKADPYVVL MKKSETKVKTRVVHDTVNPVWNQTFDFLVEDALHDML++E
Sbjct: 481 AEDLPAVDFMGKADPYVVLTMKKSETKVKTRVVHDTVNPVWNQTFDFLVEDALHDMLMIE 540

Query: 541 VWDHDTFGKDKLGRVIMTLTRAILEGEIQDNFPLEGAKSGRVFLHLKWAAQPMFRDT 598
           VWDHDTFGKDKLGRVIMTLTRAILEGEIQD+FPLEGAKSGR+FLHLKWAAQP+FRDT
Sbjct: 541 VWDHDTFGKDKLGRVIMTLTRAILEGEIQDSFPLEGAKSGRIFLHLKWAAQPIFRDT 569

BLAST of Cucsa.310180 vs. NCBI nr
Match: gi|1000974680|ref|XP_015572574.1| (PREDICTED: synaptotagmin-4 [Ricinus communis])

HSP 1 Score: 886.7 bits (2290), Expect = 2.2e-254
Identity = 439/597 (73.53%), Postives = 507/597 (84.92%), Query Frame = 1

Query: 1   MSFFSGIFLGVVVGVLLVIAFARAGNARAKHRSDLATTIAAFARMTAQDSRKILPKEFYP 60
           MSF  G+ +G+ +G+ LV+A+AR  N R+  RS LA T+AAFARM  QDSRK+LP E YP
Sbjct: 1   MSFLVGMIIGMSIGIGLVVAYARYQNIRSLRRSQLAKTVAAFARMKVQDSRKLLPGESYP 60

Query: 61  SWVVFTQRQKLTWLNLQLDKIWPYVDAAASELIRSNVEPVLEEFRPVILSSLKFSKLTLG 120
           SWVVF+QRQKLTWLNLQLDK+WPYV+ AASELIR+NVEPVLE++RP ILSSLKFSKLTLG
Sbjct: 61  SWVVFSQRQKLTWLNLQLDKVWPYVNEAASELIRTNVEPVLEQYRPAILSSLKFSKLTLG 120

Query: 121 TVAPSFTGISVLEDEPDTGGITLELEMQWDGNPNIVLDIKTKLGVSLPVQVKDIAFTGLF 180
           TVAP FTGIS+LEDE   G +T+ELEMQWDGNPNIVLDI T++GV+LP+QVK+I FTG+F
Sbjct: 121 TVAPQFTGISILEDESGAGSVTMELEMQWDGNPNIVLDINTRVGVALPIQVKNIGFTGVF 180

Query: 181 RLIFKPLVDEFPCFGAVCYSLRKKKNLDFKLKIIGGDISSIPGVSDAIEETIRDAIEGTI 240
           RLIFKPLV+EFP FGA+ YSLR KK LDFKLK++GG+IS+IPG+SDAIEETIRDAIE +I
Sbjct: 181 RLIFKPLVEEFPGFGAISYSLRHKKKLDFKLKVVGGEISAIPGISDAIEETIRDAIEDSI 240

Query: 241 TWPVRKIVPILAGDYSDLEVKPVGTLEVKLVQAKELTNKDIIGKSDPYAVLFVRPLKERM 300
           TWPVRKIVPIL GDYSDLEVKPVGTLEVKLVQ KELTNKDIIGKSDPYAV+F+RPL++RM
Sbjct: 241 TWPVRKIVPILPGDYSDLEVKPVGTLEVKLVQGKELTNKDIIGKSDPYAVVFIRPLRDRM 300

Query: 301 KTSKTINNQLNPIWNEHFNFIVEDASTQHLTIRVFDDEGVQASELIGCAQVALKDLEPGK 360
           KTSK INNQLNP+WNEHF FIVED STQHLT+RVFDDEGVQASE IGCAQVALKDLEPGK
Sbjct: 301 KTSKVINNQLNPLWNEHFEFIVEDPSTQHLTVRVFDDEGVQASEFIGCAQVALKDLEPGK 360

Query: 361 VKDVWLKLVKDLEIQRDNKYRGQVRNPLHRFQLNLGCIKQSNLLNFYACTQVHLELLYYP 420
           VKDVWLKLVKDLE+QRD KYRGQV+                            LELLY P
Sbjct: 361 VKDVWLKLVKDLEVQRDTKYRGQVQ----------------------------LELLYCP 420

Query: 421 YGTDQSLYINPFNPDYALTSVEKALKMAPSGSEDADSGKPSSPKKRDTIVRGVLSVTVIA 480
           +GT+ SL  NPFNPDY LT++EKA+K     ++DA  G+ +S KK   IVRGVLSV+V+A
Sbjct: 421 FGTESSLK-NPFNPDYQLTTLEKAIKSGAEAADDATLGRSNSQKK-SVIVRGVLSVSVVA 480

Query: 481 AEDLPAVDFMGKADPYVVLIMKKSETKVKTRVVHDTVNPVWNQTFDFLVEDALHDMLIVE 540
           AE+LPAVD MGKADPYVVL MKKSETKVKTRVV++++NPVWNQTFDF+VEDALHD+LI+E
Sbjct: 481 AENLPAVDLMGKADPYVVLQMKKSETKVKTRVVNESLNPVWNQTFDFVVEDALHDLLILE 540

Query: 541 VWDHDTFGKDKLGRVIMTLTRAILEGEIQDNFPLEGAKSGRVFLHLKWAAQPMFRDT 598
           VWDHDTFGKDK+GRVIMTLTR ILEGE Q++FP++GAKSG++FLHLKW  Q  FR+T
Sbjct: 541 VWDHDTFGKDKIGRVIMTLTRVILEGEFQESFPIDGAKSGKLFLHLKWTPQLKFRET 567

BLAST of Cucsa.310180 vs. NCBI nr
Match: gi|1009108785|ref|XP_015886685.1| (PREDICTED: synaptotagmin-5-like [Ziziphus jujuba])

HSP 1 Score: 877.9 bits (2267), Expect = 1.0e-251
Identity = 433/597 (72.53%), Postives = 501/597 (83.92%), Query Frame = 1

Query: 1   MSFFSGIFLGVVVGVLLVIAFARAGNARAKHRSDLATTIAAFARMTAQDSRKILPKEFYP 60
           MSF SG+ +G  +GV L+I FARA ++R+  RSDLA T+AAFAR+T QDSRK+LP E  P
Sbjct: 1   MSFLSGMIIGFAIGVALIIVFARATSSRSIRRSDLAKTVAAFARLTVQDSRKLLPNELCP 60

Query: 61  SWVVFTQRQKLTWLNLQLDKIWPYVDAAASELIRSNVEPVLEEFRPVILSSLKFSKLTLG 120
            WVVF QRQKLTWLN  L KIWPYV+ AASELI++NVEPVLE++RP ILSSLKFSKLTLG
Sbjct: 61  PWVVFVQRQKLTWLNSHLHKIWPYVNEAASELIKNNVEPVLEQYRPAILSSLKFSKLTLG 120

Query: 121 TVAPSFTGISVLEDEPDTGGITLELEMQWDGNPNIVLDIKTKLGVSLPVQVKDIAFTGLF 180
           TVAP FTG+S++E+E +  G+T+ELEMQWDGNPN+VLD+KT++GV LP+QVK+I FTG+F
Sbjct: 121 TVAPQFTGVSIVEEESNEKGVTMELEMQWDGNPNLVLDVKTRVGVVLPIQVKNIGFTGVF 180

Query: 181 RLIFKPLVDEFPCFGAVCYSLRKKKNLDFKLKIIGGDISSIPGVSDAIEETIRDAIEGTI 240
           RLIFKPLVDEFPCFGAVCYSLR+KK LDFKLK++GGDISSIPG+SDAIEETIRDA+E +I
Sbjct: 181 RLIFKPLVDEFPCFGAVCYSLREKKKLDFKLKVVGGDISSIPGISDAIEETIRDAVEDSI 240

Query: 241 TWPVRKIVPILAGDYSDLEVKPVGTLEVKLVQAKELTNKDIIGKSDPYAVLFVRPLKERM 300
           +WPVRKIVPIL GDYSDLE+KPVGTLEVKLVQAK LTNKD++GKSDPYAVLF+RPL+E+ 
Sbjct: 241 SWPVRKIVPILPGDYSDLELKPVGTLEVKLVQAKNLTNKDLVGKSDPYAVLFIRPLREKT 300

Query: 301 KTSKTINNQLNPIWNEHFNFIVEDASTQHLTIRVFDDEGVQASELIGCAQVALKDLEPGK 360
           KTSKTINNQLNPIWNEH+ FIV+D STQHLT+R+FDDEG+QASE IGCAQVAL+DLEPGK
Sbjct: 301 KTSKTINNQLNPIWNEHYEFIVDDVSTQHLTVRIFDDEGIQASEFIGCAQVALRDLEPGK 360

Query: 361 VKDVWLKLVKDLEIQRDNKYRGQVRNPLHRFQLNLGCIKQSNLLNFYACTQVHLELLYYP 420
           VK VWLKLVKDLEIQRD K RG                            QVHLELLY P
Sbjct: 361 VKVVWLKLVKDLEIQRDTKDRG----------------------------QVHLELLYCP 420

Query: 421 YGTDQSLYINPFNPDYALTSVEKALKMAPSGSEDADSGKPSSPKKRDTIVRGVLSVTVIA 480
           +G   + + NPFNPD+ALTS+EKALK   +G+E      P   KK+D IVRGVLSVTVI+
Sbjct: 421 FGA-ANTFKNPFNPDFALTSLEKALKSEANGTE------PGHKKKKDVIVRGVLSVTVIS 480

Query: 481 AEDLPAVDFMGKADPYVVLIMKKSETKVKTRVVHDTVNPVWNQTFDFLVEDALHDMLIVE 540
           AE LP VD +GKADPYVVLIMKKSETK KTRV+ +T+NPVWNQTFDF+V DALHDMLI+E
Sbjct: 481 AEGLPQVDLIGKADPYVVLIMKKSETKFKTRVLTETLNPVWNQTFDFVVGDALHDMLIIE 540

Query: 541 VWDHDTFGKDKLGRVIMTLTRAILEGEIQDNFPLEGAKSGRVFLHLKWAAQPMFRDT 598
           VWDHDTFGKDKLGRVIMTLTRAILEGE QD+FPLE AKSGR+ LHLKW  QP++RD+
Sbjct: 541 VWDHDTFGKDKLGRVIMTLTRAILEGEFQDSFPLEDAKSGRLNLHLKWTLQPIYRDS 562

BLAST of Cucsa.310180 vs. NCBI nr
Match: gi|731385921|ref|XP_010648678.1| (PREDICTED: synaptotagmin-5 isoform X1 [Vitis vinifera])

HSP 1 Score: 873.2 bits (2255), Expect = 2.5e-250
Identity = 433/597 (72.53%), Postives = 504/597 (84.42%), Query Frame = 1

Query: 1   MSFFSGIFLGVVVGVLLVIAFARAGNARAKHRSDLATTIAAFARMTAQDSRKILPKEFYP 60
           MSFF GI +G+  G+ +V+ FAR  N R+K R DLATT+AAFARMT QDSRKILP +FYP
Sbjct: 1   MSFFFGIVIGIACGIAVVVVFARLANLRSKSRCDLATTVAAFARMTVQDSRKILPPQFYP 60

Query: 61  SWVVFTQRQKLTWLNLQLDKIWPYVDAAASELIRSNVEPVLEEFRPVILSSLKFSKLTLG 120
           SWVVF+Q+QKLTWLN  L KIWPYVD AASELIRSNVEP+LE++RPVILS+LKFSKLTLG
Sbjct: 61  SWVVFSQQQKLTWLNSHLRKIWPYVDEAASELIRSNVEPILEQYRPVILSALKFSKLTLG 120

Query: 121 TVAPSFTGISVLEDEPDTGGITLELEMQWDGNPNIVLDIKTKLGVSLPVQVKDIAFTGLF 180
           TVAP FTG+S+LE E   G +++ELEMQWDGNPNIVLDIKT++GV LPVQVK+I FTG+F
Sbjct: 121 TVAPQFTGVSILEGE--AGEVSMELEMQWDGNPNIVLDIKTRVGVGLPVQVKNIGFTGVF 180

Query: 181 RLIFKPLVDEFPCFGAVCYSLRKKKNLDFKLKIIGGDISSIPGVSDAIEETIRDAIEGTI 240
           RLIFKP+V+EFPCFGAVCYSLR+KKNLDFKLK++GGDI+++PG+SDAIEETI DAIE +I
Sbjct: 181 RLIFKPMVEEFPCFGAVCYSLREKKNLDFKLKVVGGDITALPGISDAIEETILDAIEDSI 240

Query: 241 TWPVRKIVPILAGDYSDLEVKPVGTLEVKLVQAKELTNKDIIGKSDPYAVLFVRPLKERM 300
           TWPVRKIVPI+ GDYSDLE+KPVGTLEVKLVQA++LTNKD+IGKSDPYAVLFVRP+++RM
Sbjct: 241 TWPVRKIVPIIPGDYSDLELKPVGTLEVKLVQARDLTNKDLIGKSDPYAVLFVRPIRDRM 300

Query: 301 KTSKTINNQLNPIWNEHFNFIVEDASTQHLTIRVFDDEGVQASELIGCAQVALKDLEPGK 360
           KTSKTINN+LNPIWNEHF FIVEDASTQHLT+R+FDDEGVQASELIGCAQV LKDLEPGK
Sbjct: 301 KTSKTINNELNPIWNEHFEFIVEDASTQHLTVRIFDDEGVQASELIGCAQVRLKDLEPGK 360

Query: 361 VKDVWLKLVKDLEIQRDNKYRGQVRNPLHRFQLNLGCIKQSNLLNFYACTQVHLELLYYP 420
           VKDVWLKLVKDL++QRD KYRG+VR                            LELLY P
Sbjct: 361 VKDVWLKLVKDLDVQRDKKYRGEVR----------------------------LELLYCP 420

Query: 421 YGTDQSLYINPFNPDYALTSVEKALKMAPSGSEDADSGKPSSPKKRDTIVRGVLSVTVIA 480
           +G  +S++ NPF P+  LTS+EK LK    G+E  D  K  S KKRD IVRGVLSVTVI+
Sbjct: 421 FGM-ESVFTNPFRPN-LLTSLEKVLK--ADGTEADDIKKSHSLKKRDIIVRGVLSVTVIS 480

Query: 481 AEDLPAVDFMGKADPYVVLIMKKSETKVKTRVVHDTVNPVWNQTFDFLVEDALHDMLIVE 540
           AE+LP VD +GKADPYV L MKKS TK +TRVV++++NP+WNQTFDF+VED LHDMLI++
Sbjct: 481 AENLPVVDLIGKADPYVELTMKKSNTKHRTRVVNNSLNPIWNQTFDFVVEDGLHDMLILD 540

Query: 541 VWDHDTFGKDKLGRVIMTLTRAILEGEIQDNFPLEGAKSGRVFLHLKWAAQPMFRDT 598
           VWDHDTFGKDK+GR I TLTR ILEGE +DNFPLEGAKSG + LHLKW+ QP+FRDT
Sbjct: 541 VWDHDTFGKDKIGRCIFTLTRVILEGEFRDNFPLEGAKSGSLNLHLKWSPQPIFRDT 563