Cucsa.306610 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.306610 vs. Swiss-Prot
Match: NFD4_ARATH (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana GN=NFD4 PE=3 SV=1)

HSP 1 Score: 155.6 bits (392), Expect = 1.6e-36
Identity = 135/547 (24.68%), Postives = 247/547 (45.16%), Query Frame = 1

Query: 19  LFGRWFS---VLASILIMSVAGATYMFALYSSDIKSSLGYDQTTLNLLSFFKDLGGNVGV 78
           +FGRW     ++A+I I +  G  + F+ YSS +KS LG  Q  LN L+   DLG   G 
Sbjct: 37  MFGRWRKWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGW 96

Query: 79  ISGLTNEVAPAWVVLLIGAVMNLFGYTMIWLAVTKRIPKPQ--IWHMCLYITIGANSQTF 138
            SG+     P  VVL   A M   GY + WL +T  I  P   ++  CL   +   S  +
Sbjct: 97  SSGIALGYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLPYSLVFLCCL---LAGLSICW 156

Query: 139 ANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLYRAFYGNNPESLILLIAWLPAA 198
            NT   + C+++FP +R   L L   F G+S A+ S  + A   ++    +LL + +P  
Sbjct: 157 FNTACFILCIRHFPNNRALALSLTVSFNGISAALYSLAFNAINPSSSNLYLLLNSLVPLV 216

Query: 199 VSVVLLRFVRIIKDLRQPNELKVFYH----FLYISLGLAGTLMVLIILQSLLRFQQIQYV 258
           VS   L  V     L    +     H    F  +++    T   L++  S     ++ ++
Sbjct: 217 VSFAALYPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSSTSSARLNFI 276

Query: 259 GSAIVVIVLLLLPLTIVFREELSVWKSKIASPVLQLESASQQPPPPLTSTVSL--APSSP 318
           G+ ++++  L  PL +  R+           PV+      +     + +   L    +S 
Sbjct: 277 GAVVLLVFPLCAPLLVYARDYF--------LPVINARLNHESSGYVMLNIDELKNQKTSV 336

Query: 319 PSESCFKNM-----FNPPSRGEDYTIPQAIFSMDLIILFMATICGVGGTLTAIDNLGQIG 378
            S++ +++M      N    G++++    I  ++  + ++A  CG    L   +NLGQI 
Sbjct: 337 SSKTGYEHMGTAKEGNTVRLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIA 396

Query: 379 ESLGYQSHSITTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGHLL 438
           +SLG  S   TT +++ S +++ GR++S    ++  K++++ R  +    L+ + +   L
Sbjct: 397 QSLGQNS---TTLVTIYSSFSFFGRLLSA-APDFMHKRFRLTRTGWFAIALLPTPIAFFL 456

Query: 439 IAF--GVPNSLYFSSIVIGFCFGAQWPLIYAIISEIFGLKYYATLSSMSGGASPIGAYIL 498
           +A       +L  ++ +IG   G  +    +I S++FG        ++     PIG+ +L
Sbjct: 457 LAVSSSQQTALQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGS-LL 516

Query: 499 NVRVAGHLYDREAQRQMEATGRRRNIGEDLSCLGVECYRKAFLIITAATVFGALVSLILV 548
              +A  +Y+  A   +        + + + C+G +CY K F+     ++ G + SL L 
Sbjct: 517 YGYIAASIYEANASPDITPI-----VSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLY 562

BLAST of Cucsa.306610 vs. TrEMBL
Match: A0A0A0LEA4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G742600 PE=4 SV=1)

HSP 1 Score: 1146.0 bits (2963), Expect = 0.0e+00
Identity = 580/580 (100.00%), Postives = 580/580 (100.00%), Query Frame = 1

Query: 1   MAASSSNPTSSNPFWYQFLFGRWFSVLASILIMSVAGATYMFALYSSDIKSSLGYDQTTL 60
           MAASSSNPTSSNPFWYQFLFGRWFSVLASILIMSVAGATYMFALYSSDIKSSLGYDQTTL
Sbjct: 1   MAASSSNPTSSNPFWYQFLFGRWFSVLASILIMSVAGATYMFALYSSDIKSSLGYDQTTL 60

Query: 61  NLLSFFKDLGGNVGVISGLTNEVAPAWVVLLIGAVMNLFGYTMIWLAVTKRIPKPQIWHM 120
           NLLSFFKDLGGNVGVISGLTNEVAPAWVVLLIGAVMNLFGYTMIWLAVTKRIPKPQIWHM
Sbjct: 61  NLLSFFKDLGGNVGVISGLTNEVAPAWVVLLIGAVMNLFGYTMIWLAVTKRIPKPQIWHM 120

Query: 121 CLYITIGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLYRAFYGNNP 180
           CLYITIGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLYRAFYGNNP
Sbjct: 121 CLYITIGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLYRAFYGNNP 180

Query: 181 ESLILLIAWLPAAVSVVLLRFVRIIKDLRQPNELKVFYHFLYISLGLAGTLMVLIILQSL 240
           ESLILLIAWLPAAVSVVLLRFVRIIKDLRQPNELKVFYHFLYISLGLAGTLMVLIILQSL
Sbjct: 181 ESLILLIAWLPAAVSVVLLRFVRIIKDLRQPNELKVFYHFLYISLGLAGTLMVLIILQSL 240

Query: 241 LRFQQIQYVGSAIVVIVLLLLPLTIVFREELSVWKSKIASPVLQLESASQQPPPPLTSTV 300
           LRFQQIQYVGSAIVVIVLLLLPLTIVFREELSVWKSKIASPVLQLESASQQPPPPLTSTV
Sbjct: 241 LRFQQIQYVGSAIVVIVLLLLPLTIVFREELSVWKSKIASPVLQLESASQQPPPPLTSTV 300

Query: 301 SLAPSSPPSESCFKNMFNPPSRGEDYTIPQAIFSMDLIILFMATICGVGGTLTAIDNLGQ 360
           SLAPSSPPSESCFKNMFNPPSRGEDYTIPQAIFSMDLIILFMATICGVGGTLTAIDNLGQ
Sbjct: 301 SLAPSSPPSESCFKNMFNPPSRGEDYTIPQAIFSMDLIILFMATICGVGGTLTAIDNLGQ 360

Query: 361 IGESLGYQSHSITTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGH 420
           IGESLGYQSHSITTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGH
Sbjct: 361 IGESLGYQSHSITTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGH 420

Query: 421 LLIAFGVPNSLYFSSIVIGFCFGAQWPLIYAIISEIFGLKYYATLSSMSGGASPIGAYIL 480
           LLIAFGVPNSLYFSSIVIGFCFGAQWPLIYAIISEIFGLKYYATLSSMSGGASPIGAYIL
Sbjct: 421 LLIAFGVPNSLYFSSIVIGFCFGAQWPLIYAIISEIFGLKYYATLSSMSGGASPIGAYIL 480

Query: 481 NVRVAGHLYDREAQRQMEATGRRRNIGEDLSCLGVECYRKAFLIITAATVFGALVSLILV 540
           NVRVAGHLYDREAQRQMEATGRRRNIGEDLSCLGVECYRKAFLIITAATVFGALVSLILV
Sbjct: 481 NVRVAGHLYDREAQRQMEATGRRRNIGEDLSCLGVECYRKAFLIITAATVFGALVSLILV 540

Query: 541 VRTWKFYKGDIYRKFREVEGEDIEMKMAAPTNGTLTAAKN 581
           VRTWKFYKGDIYRKFREVEGEDIEMKMAAPTNGTLTAAKN
Sbjct: 541 VRTWKFYKGDIYRKFREVEGEDIEMKMAAPTNGTLTAAKN 580

BLAST of Cucsa.306610 vs. TrEMBL
Match: F6HKC4_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_08s0007g04860 PE=4 SV=1)

HSP 1 Score: 770.4 bits (1988), Expect = 1.6e-219
Identity = 387/575 (67.30%), Postives = 459/575 (79.83%), Query Frame = 1

Query: 14  FWYQFLFGRWFSVLASILIMSVAGATYMFALYSSDIKSSLGYDQTTLNLLSFFKDLGGNV 73
           F  Q L GRWF V AS+LIMSVAGATYMF+LYS +IK+SLGYDQTTLNLLSFFKDLGGNV
Sbjct: 18  FGLQVLVGRWFMVFASLLIMSVAGATYMFSLYSGEIKTSLGYDQTTLNLLSFFKDLGGNV 77

Query: 74  GVISGLTNEVAPAWVVLLIGAVMNLFGYTMIWLAVTKRIPKPQIWHMCLYITIGANSQTF 133
           GV+SGL NEV P WVVL IGAVMN FGY MIWLAVT RI KPQIW MCLYI IGANSQ+F
Sbjct: 78  GVLSGLINEVTPPWVVLCIGAVMNFFGYFMIWLAVTDRIAKPQIWQMCLYICIGANSQSF 137

Query: 134 ANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLYRAFYGNNPESLILLIAWLPAA 193
           ANTGALVTCV+NFPESRG VLGLLKGFVGLSGAI++QLYRAFYG++ +SLIL IAWLPAA
Sbjct: 138 ANTGALVTCVRNFPESRGIVLGLLKGFVGLSGAIITQLYRAFYGDDSKSLILFIAWLPAA 197

Query: 194 VSVVLLRFVRIIKDLRQPNELKVFYHFLYISLGLAGTLMVLIILQSLLRFQQIQYVGSAI 253
           VS V LR +RI+K  RQ NELKVFY  LY+SLGLAG LMV+II+Q+   F +I+Y GSA 
Sbjct: 198 VSFVFLRTIRIMKVGRQANELKVFYDLLYMSLGLAGFLMVIIIIQNKFTFSRIEYSGSAA 257

Query: 254 VVIVLLLLPLTIVFREELSVWK-SKIASPVLQLESASQQPP------PPLTSTVSLAP-- 313
           VV++LL LPL +V +EE+++WK  K A    Q++  ++ PP       P+ S   L P  
Sbjct: 258 VVLILLFLPLAVVIKEEINIWKGKKQALDAAQVKVITENPPAVELASSPVVSLDQLPPPT 317

Query: 314 ----SSPPSESCFKNMFNPPSRGEDYTIPQAIFSMDLIILFMATICGVGGTLTAIDNLGQ 373
               ++  S SCFK MF PP RGEDYTI QA+FS+D++ILF+ T CGVGGTLTAIDNLGQ
Sbjct: 318 AAPENAEKSVSCFKTMFKPPDRGEDYTILQALFSIDMLILFIVTTCGVGGTLTAIDNLGQ 377

Query: 374 IGESLGYQSHSITTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGH 433
           IG S GY +HS TTF+SLVSIWNYLGRVVSGF SE F  +YK PRPL L   L+ SCVGH
Sbjct: 378 IGSSQGYTAHSTTTFVSLVSIWNYLGRVVSGFASEIFLTRYKFPRPLMLTFVLLFSCVGH 437

Query: 434 LLIAFGVPNSLYFSSIVIGFCFGAQWPLIYAIISEIFGLKYYATLSSMSGGASPIGAYIL 493
           LLIAF VPNSLYF+S++IGFCFGAQWPL++AIISE+FGLKYY+TL +    ASP+G+YIL
Sbjct: 438 LLIAFAVPNSLYFASVIIGFCFGAQWPLVFAIISELFGLKYYSTLYNFGAVASPVGSYIL 497

Query: 494 NVRVAGHLYDREAQRQMEATGRRRNIGEDLSCLGVECYRKAFLIITAATVFGALVSLILV 553
           NV+VAGHLYD+EA +Q+EA+G  R  G+DL+C G  CY+ +F+IITAAT+FG ++S ILV
Sbjct: 498 NVKVAGHLYDKEALKQLEASGVTRVAGQDLTCTGAACYKLSFIIITAATLFGCIISFILV 557

Query: 554 VRTWKFYKGDIYRKFREVEGEDIEMKMAAPTNGTL 576
           +RT KFY+GDIY+KFR+ E    E+ MA+  NG L
Sbjct: 558 IRTKKFYQGDIYKKFRQ-EANKAEINMASSGNGIL 591

BLAST of Cucsa.306610 vs. TrEMBL
Match: A0A067KBI7_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_16568 PE=4 SV=1)

HSP 1 Score: 762.7 bits (1968), Expect = 3.3e-217
Identity = 376/548 (68.61%), Postives = 452/548 (82.48%), Query Frame = 1

Query: 14  FWYQFLFGRWFSVLASILIMSVAGATYMFALYSSDIKSSLGYDQTTLNLLSFFKDLGGNV 73
           F+ + + GRWF V ASILIM+VAGATYMF LYS+DIK+SLGYDQTTLNLLSFFKDLGGNV
Sbjct: 19  FFLKMILGRWFMVFASILIMAVAGATYMFGLYSNDIKTSLGYDQTTLNLLSFFKDLGGNV 78

Query: 74  GVISGLTNEVAPAWVVLLIGAVMNLFGYTMIWLAVTKRIPKPQIWHMCLYITIGANSQTF 133
           GV+SGL NEV P WVVL IGA+MN FGY MIWLAVT  IPKP++W MCLYI IGANSQ F
Sbjct: 79  GVLSGLINEVTPPWVVLSIGAIMNFFGYFMIWLAVTGHIPKPKVWQMCLYICIGANSQAF 138

Query: 134 ANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLYRAFYGNNPESLILLIAWLPAA 193
           ANTGALVTCVKNFPESRG+VLGLLK FVGLSGAI +Q+Y A YG + +SLILLIAWLPAA
Sbjct: 139 ANTGALVTCVKNFPESRGAVLGLLKSFVGLSGAIFTQIYHALYGYDSKSLILLIAWLPAA 198

Query: 194 VSVVLLRFVRIIKDLRQPNELKVFYHFLYISLGLAGTLMVLIILQSLLRFQQIQYVGSAI 253
           +S + LR +RII+ +RQ NELK+FY  LYISLGLAG LMVLII+Q+   F +I+Y+GS+ 
Sbjct: 199 ISFIFLRTIRIIEVVRQANELKIFYKLLYISLGLAGFLMVLIIMQNKFNFTRIEYIGSSS 258

Query: 254 VVIVLLLLPLTIVFREELSVWKSKIASPVLQ--LESASQQPPPPLTSTVSLAPSSPP--S 313
           +V++LL LP+ +V +EE  V KSK A+   Q  ++  +Q+ P    S+V ++ SSPP   
Sbjct: 259 IVLLLLFLPIVVVIKEEYHVRKSKKAALANQSSIKVITQENP----SSVEVSSSSPPRAQ 318

Query: 314 ESCFKNMFNPPSRGEDYTIPQAIFSMDLIILFMATICGVGGTLTAIDNLGQIGESLGYQS 373
            SC  N+FNPP+RGEDYTI QA+FS+D++ILF+AT CGVGGTLTA+DNLGQIG SLGY S
Sbjct: 319 PSCTNNIFNPPNRGEDYTILQALFSVDMLILFIATTCGVGGTLTAVDNLGQIGNSLGYPS 378

Query: 374 HSITTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGHLLIAFGVPN 433
           HS TTF+SLVSIWNYLGR V+GF SE+   KYK+PRPL     L+ SCVGHLLIAFGVPN
Sbjct: 379 HSTTTFVSLVSIWNYLGRAVAGFASEFLLTKYKIPRPLLFTLVLLFSCVGHLLIAFGVPN 438

Query: 434 SLYFSSIVIGFCFGAQWPLIYAIISEIFGLKYYATLSSMSGGASPIGAYILNVRVAGHLY 493
           SLYF+S++IGFCFGAQWPL++AIISEIFGLKYY+TL +    ASP+G+YILNVRVAGHLY
Sbjct: 439 SLYFASVIIGFCFGAQWPLLFAIISEIFGLKYYSTLYNFGSVASPVGSYILNVRVAGHLY 498

Query: 494 DREAQRQMEATGRRRNIGEDLSCLGVECYRKAFLIITAATVFGALVSLILVVRTWKFYKG 553
           D+EA +Q+E  G +R  G+ L+C GV+CY+ AFLIITAAT+FG ++S ILVVRT KFY+G
Sbjct: 499 DKEALKQIEGLGLKREAGKYLTCSGVQCYKMAFLIITAATLFGCVISSILVVRTRKFYQG 558

Query: 554 DIYRKFRE 558
           DIY+KFRE
Sbjct: 559 DIYKKFRE 562

BLAST of Cucsa.306610 vs. TrEMBL
Match: A0A061ESC1_THECC (Major facilitator superfamily protein OS=Theobroma cacao GN=TCM_022288 PE=4 SV=1)

HSP 1 Score: 760.0 bits (1961), Expect = 2.1e-216
Identity = 393/594 (66.16%), Postives = 457/594 (76.94%), Query Frame = 1

Query: 14  FWYQFLFGRWFSVLASILIMSVAGATYMFALYSSDIKSSLGYDQTTLNLLSFFKDLGGNV 73
           F    + GRWF V AS+LIMSV+GATYMF LYS+ IKSSLGYDQTTLNLLSF KDLGGNV
Sbjct: 19  FGLNLIIGRWFMVFASLLIMSVSGATYMFGLYSTVIKSSLGYDQTTLNLLSFSKDLGGNV 78

Query: 74  GVISGLTNEVAPAWVVLLIGAVMNLFGYTMIWLAVTKRIPKPQIWHMCLYITIGANSQTF 133
           GV+SGL NEV P WVVLLIG+VMN FGY MIWLAVT R  KP +W MC+YI +GANSQ F
Sbjct: 79  GVLSGLINEVTPPWVVLLIGSVMNFFGYFMIWLAVTGRTAKPHVWQMCVYICLGANSQAF 138

Query: 134 ANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLYRAFYGNNPESLILLIAWLPAA 193
           ANTGALVTCVKNFPESRGSVLGLLK +VGLSGAIL+QLY AFYG N +SLILLIAWLPAA
Sbjct: 139 ANTGALVTCVKNFPESRGSVLGLLKSYVGLSGAILTQLYHAFYGENSKSLILLIAWLPAA 198

Query: 194 VSVVLLRFVRIIKDLRQPNELKVFYHFLYISLGLAGTLMVLIILQSLLRFQQIQYVGSAI 253
           VS V LR +RIIK +RQ NEL+VFY+  YISL LAG LMVLI++Q+ L F +I+YVGSA 
Sbjct: 199 VSFVFLRTIRIIKIVRQTNELRVFYNMFYISLALAGFLMVLIVIQNRLSFNRIEYVGSAS 258

Query: 254 VVIVLLLLPLTIVFREELSVW--KSKIASPVLQLESASQQPPPPLTSTVSLAPSSPPSES 313
           +V +LLL PLT+V RE+  +W  K +  + V Q++  ++ PP     TV LAP +   ES
Sbjct: 259 LVTLLLLFPLTVVVREDFKIWRRKKQALNDVSQMKVVTENPP-----TVELAPQTMAVES 318

Query: 314 ------------------CFKNMFNPPSRGEDYTIPQAIFSMDLIILFMATICGVGGTLT 373
                             C +N+F PP RGEDYTI QA+FS+D++ILF+AT CGVGGTLT
Sbjct: 319 LQPQPAGDPKSPDQKHVACMENIFKPPDRGEDYTILQALFSIDMLILFIATTCGVGGTLT 378

Query: 374 AIDNLGQIGESLGYQSHSITTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATL 433
           AIDNLGQIG SLGY S SITTF+SLVSIWNYLGR V+GF SEY   KYK+PRPL   + +
Sbjct: 379 AIDNLGQIGNSLGYPSRSITTFVSLVSIWNYLGRAVAGFFSEYLLTKYKIPRPLLFTSVI 438

Query: 434 ILSCVGHLLIAFGVPNSLYFSSIVIGFCFGAQWPLIYAIISEIFGLKYYATLSSMSGGAS 493
           + SCVGHLLIAF VPNSLYF+S++IGFCFGAQWPL+YAIISEIFGLKYY+TL +    AS
Sbjct: 439 LFSCVGHLLIAFAVPNSLYFASVIIGFCFGAQWPLMYAIISEIFGLKYYSTLYNFGAVAS 498

Query: 494 PIGAYILNVRVAGHLYDREAQRQMEATGRRRNIGEDLSCLGVECYRKAFLIITAATVFGA 553
           P+G+YILNVRVAGHLYD+EA +QM+A GR R  GEDL+C G +CYR AF+II A T+FG 
Sbjct: 499 PVGSYILNVRVAGHLYDKEALKQMKALGRTRKPGEDLTCTGGQCYRMAFIIIIATTLFGF 558

Query: 554 LVSLILVVRTWKFYKGDIYRKFREVEGEDIEMKMA---------APTN-GTLTA 578
           LVS ILV+RT KFYK DIY+KFRE E E  E  MA         APT+ GT TA
Sbjct: 559 LVSCILVLRTRKFYKSDIYKKFRE-EAEAAETDMASTSKQVEPKAPTDAGTTTA 606

BLAST of Cucsa.306610 vs. TrEMBL
Match: M5VWK9_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa002810mg PE=4 SV=1)

HSP 1 Score: 756.5 bits (1952), Expect = 2.3e-215
Identity = 382/566 (67.49%), Postives = 456/566 (80.57%), Query Frame = 1

Query: 14  FWYQFLFGRWFSVLASILIMSVAGATYMFALYSSDIKSSLGYDQTTLNLLSFFKDLGGNV 73
           F  Q + GRWF V AS+LI+SVAGATYMFALYS+DIK SLGYDQTT+NLLSFFKDLGGNV
Sbjct: 21  FGLQVVHGRWFMVFASMLILSVAGATYMFALYSNDIKKSLGYDQTTINLLSFFKDLGGNV 80

Query: 74  GVISGLTNEVAPAWVVLLIGAVMNLFGYTMIWLAVTKRIPKPQIWHMCLYITIGANSQTF 133
           GVI+GL NE    WVVLLIGAVMN FGY MIWLAVT R  KP++W MCLYI IGANSQTF
Sbjct: 81  GVIAGLINEFTAPWVVLLIGAVMNFFGYFMIWLAVTGRTSKPRVWQMCLYICIGANSQTF 140

Query: 134 ANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLYRAFYGNNPESLILLIAWLPAA 193
           +NTGA+V+CVKNFPESRGSVLGLLKG VGLSGAI++QL+ AFYG + ++LILLI WLPAA
Sbjct: 141 SNTGAVVSCVKNFPESRGSVLGLLKGLVGLSGAIITQLFLAFYGRDSKALILLIGWLPAA 200

Query: 194 VSVVLLRFVRIIKDLRQPNELKVFYHFLYISLGLAGTLMVLIILQSLLRFQQIQYVGSAI 253
           VS+V L  +R++K +RQPNEL+VFY+ LY++LGLAG +M LII+Q  LRF +I+Y+GSA 
Sbjct: 201 VSMVFLPIIRLLKVVRQPNELRVFYNLLYVTLGLAGFIMFLIIIQHKLRFSRIEYIGSAS 260

Query: 254 VVIVLLLLPLTIVFREELSVWKSKI--ASPVLQLESASQQPPPPLTSTVSLAP-SSPPS- 313
           VV+VLL LPL +V  EE  +WKSK+   +   QL+  ++ P PP+   V+L P ++PPS 
Sbjct: 261 VVLVLLFLPLAVVIFEEFKIWKSKMRALNDQSQLKVVAENPAPPV--QVALPPVAAPPSP 320

Query: 314 ------ESCFKNMFNPPSRGEDYTIPQAIFSMDLIILFMATICGVGGTLTAIDNLGQIGE 373
                  SC +N+F PP RGEDYTI QA+FS+D++ILF+A  CG GGTLTAIDNLGQIG 
Sbjct: 321 QTQQKPNSCLQNVFRPPDRGEDYTILQAVFSIDMLILFIAITCGAGGTLTAIDNLGQIGG 380

Query: 374 SLGYQSHSITTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGHLLI 433
           SLGY +HSI+TFISLVSIWNYLGRV SGF SE   KKYK PRPL L   L+LSCVGHLLI
Sbjct: 381 SLGYPTHSISTFISLVSIWNYLGRVTSGFSSEVLLKKYKFPRPLLLTLVLLLSCVGHLLI 440

Query: 434 AFGVPNSLYFSSIVIGFCFGAQWPLIYAIISEIFGLKYYATLSSMSGGASPIGAYILNVR 493
           AFGVPNSLY +S++IGFCFGAQWPLI+AIISEIFGLKYYATL ++ G ASPIGAY+LNV+
Sbjct: 441 AFGVPNSLYVASVIIGFCFGAQWPLIFAIISEIFGLKYYATLINVGGAASPIGAYLLNVK 500

Query: 494 VAGHLYDREAQRQMEATGRRRNIGEDLSCLGVECYRKAFLIITAATVFGALVSLILVVRT 553
           VAG+LYD EA +Q++A G+ R  GEDL+C GV CYR AF I+ A  +FG+LVS ILV+RT
Sbjct: 501 VAGNLYDAEALKQLKALGKVRKAGEDLTCEGVPCYRLAFCIMAAVALFGSLVSFILVIRT 560

Query: 554 WKFYKGDIYRKFREVEGEDIEMKMAA 570
            KFY+ DIY+KFRE   E  E  +AA
Sbjct: 561 RKFYRSDIYKKFREA-AEAAESDVAA 583

BLAST of Cucsa.306610 vs. TAIR10
Match: AT2G39210.1 (AT2G39210.1 Major facilitator superfamily protein)

HSP 1 Score: 721.5 bits (1861), Expect = 4.2e-208
Identity = 366/582 (62.89%), Postives = 438/582 (75.26%), Query Frame = 1

Query: 17  QFLFGRWFSVLASILIMSVAGATYMFALYSSDIKSSLGYDQTTLNLLSFFKDLGGNVGVI 76
           Q L GRWF    S+LIMS AGATYMF +YS DIK +LGYDQTTLNLLSFFKDLG NVGV+
Sbjct: 16  QILTGRWFMFFGSLLIMSTAGATYMFGIYSGDIKETLGYDQTTLNLLSFFKDLGANVGVL 75

Query: 77  SGLTNEVAPAWVVLLIGAVMNLFGYTMIWLAVTKRIPKPQIWHMCLYITIGANSQTFANT 136
           +GL NEV P W +LLIGA++N FGY MIWLAVT+RI KPQ+WHMCLYI +GANSQ+FANT
Sbjct: 76  AGLLNEVTPPWFILLIGAILNFFGYFMIWLAVTERISKPQVWHMCLYICVGANSQSFANT 135

Query: 137 GALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLYRAFYGNNPESLILLIAWLPAAVSV 196
           G+LVTCVKNFPESRG VLG+LKG+VGLSGAI++QLYRAFYG + + LIL+I WLPA VS 
Sbjct: 136 GSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLYRAFYGEDTKELILMIGWLPAIVSF 195

Query: 197 VLLRFVRIIKDLRQPNELKVFYHFLYISLGLAGTLMVLIILQSLLRFQQIQYVGSAIVVI 256
             LR +RI+K  RQ NELKVFY+FLYISLGLA  LMV+II+  L  F Q ++ GSA VVI
Sbjct: 196 AFLRTIRIMKVKRQTNELKVFYNFLYISLGLATFLMVVIIINKLSGFTQSEFGGSAAVVI 255

Query: 257 VLLLLPLTIVFREELSVWKSKIASPVLQLESASQQPPPPLTSTVSLAPSSPPSE------ 316
           VLLLLP+ +V  EE  +WK K        + A   P P   + V+  P    SE      
Sbjct: 256 VLLLLPIIVVILEEKKLWKEK--------QVALNDPAP--INVVTEKPKLDSSEFKDDDG 315

Query: 317 -------------SCFKNMFNPPSRGEDYTIPQAIFSMDLIILFMATICGVGGTLTAIDN 376
                        SC+  +FNPP RG+DYTI QA+FS+D++ILF+ATICGVGGTLTAIDN
Sbjct: 316 EESKEVVEKVKTPSCWTTVFNPPERGDDYTILQALFSVDMLILFLATICGVGGTLTAIDN 375

Query: 377 LGQIGESLGYQSHSITTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSC 436
           LGQIG SLGY   S++TF+SLVSIWNY GRVVSG VSE F  KYK PRPL L   L+LSC
Sbjct: 376 LGQIGNSLGYPKRSVSTFVSLVSIWNYYGRVVSGVVSEIFLIKYKFPRPLMLTMVLLLSC 435

Query: 437 VGHLLIAFGVPNSLYFSSIVIGFCFGAQWPLIYAIISEIFGLKYYATLSSMSGGASPIGA 496
            GHLLIAF VP  LY +S++IGFCFGAQWPL++AIISEIFGLKYY+TL +    ASPIG+
Sbjct: 436 AGHLLIAFNVPGGLYVASVIIGFCFGAQWPLLFAIISEIFGLKYYSTLYNFGSVASPIGS 495

Query: 497 YILNVRVAGHLYDREAQRQMEATGRRRNIGEDLSCLGVECYRKAFLIITAATVFGALVSL 556
           Y+LNVRVAG+LYD EA +Q +A G+ R  G+DL+C+G  C++ +F+II A T+FG LVS+
Sbjct: 496 YLLNVRVAGYLYDVEAGKQYKALGKTRVEGQDLNCIGTSCFKLSFIIIAAVTLFGVLVSM 555

Query: 557 ILVVRTWKFYKGDIYRKFREVEGEDIEMKMAAPTNGTLTAAK 580
           +LV+RT KFYK DIY+KFRE +    EM+MAAP     T AK
Sbjct: 556 VLVIRTKKFYKSDIYKKFRE-KALAAEMEMAAPAAARSTVAK 586

BLAST of Cucsa.306610 vs. TAIR10
Match: AT2G28120.1 (AT2G28120.1 Major facilitator superfamily protein)

HSP 1 Score: 655.2 bits (1689), Expect = 3.7e-188
Identity = 331/556 (59.53%), Postives = 409/556 (73.56%), Query Frame = 1

Query: 14  FWYQFLFGRWFSVLASILIMSVAGATYMFALYSSDIKSSLGYDQTTLNLLSFFKDLGGNV 73
           F   F  GRWF V AS LIM+ AGATY+F  YS DIKS+LGYDQTTLNLL FFKDLG NV
Sbjct: 9   FLIHFFNGRWFMVFASFLIMACAGATYLFGTYSKDIKSTLGYDQTTLNLLGFFKDLGANV 68

Query: 74  GVISGLTNEVAPAWVVLLIGAVMNLFGYTMIWLAVTKRIPKPQIWHMCLYITIGANSQTF 133
           GV+SGL  EV P W VL IG+ MN  GY MIWL VT ++ KP++W MCLYI IGANSQ F
Sbjct: 69  GVLSGLIAEVTPTWFVLTIGSAMNFVGYFMIWLTVTGKVAKPKVWQMCLYICIGANSQNF 128

Query: 134 ANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLYRAFYGNNPESLILLIAWLPAA 193
           ANTGALVTCVKNFPESRG +LGLLKG+VGLSGAI +QLY A YG++ +SLILLIAWLPAA
Sbjct: 129 ANTGALVTCVKNFPESRGVMLGLLKGYVGLSGAIFTQLYFAIYGHDSKSLILLIAWLPAA 188

Query: 194 VSVVLLRFVRIIKDLRQPNELKVFYHFLYISLGLAGTLMVLIILQSLLRFQQIQYVGSAI 253
           VS+V +  +R  K +RQ NEL VFY FLYIS+ LA  LM + I +  + F +  Y  SA 
Sbjct: 189 VSLVFVYLIREKKVVRQRNELSVFYQFLYISIFLALFLMAMNIAEKQVHFSKAAYAASAT 248

Query: 254 VVIVLLLLPLTIVFREELSVW---KSKIASPV-LQLESASQQPPPPLTSTVSLAPSSPPS 313
           +   LL +PLT+  ++EL VW   K  I  P  +++E   ++          +      +
Sbjct: 249 ICCALLFVPLTVSVKQELEVWNMMKLPIEEPSEVKVEKPKKELDLDQDKAAKVNGEEKET 308

Query: 314 ESCFKNMFNPPSRGEDYTIPQAIFSMDLIILFMATICGVGGTLTAIDNLGQIGESLGYQS 373
           +SCF  +F+PP RGEDYTI QA+ S D+IILF+AT CG+G +LTA+DNLGQIGESLGY +
Sbjct: 309 KSCFSTVFSPPPRGEDYTILQALLSTDMIILFVATFCGLGSSLTAVDNLGQIGESLGYPN 368

Query: 374 HSITTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGHLLIAFGVPN 433
           H++++F+SLVSIWNY GRV SGFVSEY   KYK+PRPL +   L+LSC GHLLIAF VP 
Sbjct: 369 HTVSSFVSLVSIWNYFGRVFSGFVSEYLLAKYKLPRPLMMTLVLLLSCAGHLLIAFPVPG 428

Query: 434 SLYFSSIVIGFCFGAQWPLIYAIISEIFGLKYYATLSSMSGGASPIGAYILNVRVAGHLY 493
           S+Y +SI++GF FGAQ PL++AIISE+FGLKYY+TL +    ASP+G+YILNVRV G LY
Sbjct: 429 SVYIASILMGFSFGAQLPLLFAIISELFGLKYYSTLFNCGQLASPLGSYILNVRVTGMLY 488

Query: 494 DREAQRQMEATGRRRNIGEDLSCLGVECYRKAFLIITAATVFGALVSLILVVRTWKFYKG 553
           D+EA +Q+ A G  R   +DL+CLG +CY+  FLI+ A T FGALVSL L +RT +FYKG
Sbjct: 489 DKEALKQLTARGLTRKDVKDLTCLGSQCYKLPFLILAAVTFFGALVSLGLAIRTREFYKG 548

Query: 554 DIYRKFREVEGEDIEM 566
           DIY+KFRE    + E+
Sbjct: 549 DIYKKFRESPESESEL 564

BLAST of Cucsa.306610 vs. TAIR10
Match: AT5G14120.1 (AT5G14120.1 Major facilitator superfamily protein)

HSP 1 Score: 353.2 bits (905), Expect = 3.0e-97
Identity = 212/576 (36.81%), Postives = 315/576 (54.69%), Query Frame = 1

Query: 18  FLFGRWFSVLASILIMSVAGATYMFALYSSDIKSSLGYDQTTLNLLSFFKDLGGNVGVIS 77
           F+  RW   +A++ I S AG  Y+F   S  IKSSL Y+Q  L+ L   KDLG +VG I+
Sbjct: 12  FINNRWLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKELSRLGVAKDLGDSVGFIA 71

Query: 78  GLTNEVAPAWVVLLIGAVMNLFGYTMIWLAVTKRIPKPQIWHMCLYITIGANSQTFANTG 137
           G  +E+ P W  LL+GAV NL GY  +WL VT R P   +W MC+ I +G N +T+ NTG
Sbjct: 72  GTLSEILPLWAALLVGAVQNLIGYGWVWLIVTGRAPILPLWAMCVLIFVGNNGETYFNTG 131

Query: 138 ALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLYRAFYGNNPESLILLIAWLPAAVSVV 197
           ALV+ V+NFP+SRG V+G+LKGF GL GAI+SQ+Y   + +NP SLIL++A  PA V V 
Sbjct: 132 ALVSGVQNFPKSRGPVVGILKGFAGLGGAIISQIYTMIHSSNPASLILMVAVTPAVVVVC 191

Query: 198 LLRFVRIIKDLRQ--PNELKVFYHFLYISLGLAGTLMVLIILQSLLRFQQIQYVGSAIVV 257
           L+ F+R +   +Q  P +   F     + L LA  LM ++++Q L+           IV+
Sbjct: 192 LMFFIRPVGGHKQIRPTDGASFTFIYGVCLLLAAYLMSVMLIQDLVVVSHNVITVFTIVL 251

Query: 258 IVLLLLPLTIVFR--------------EELSVWKSKIASPVLQ-----LESASQQPPPPL 317
            V+L++P+ +                 EE  V K +   P LQ     L     + P   
Sbjct: 252 FVILVVPILVPIMTSFFTETNEPDDTIEEPLVPKREDQEPGLQTPDLILSEVEDEKP--- 311

Query: 318 TSTVSLAPSS---------------PPSESCFK-NMFNPPSRGEDYTIPQAIFSMDLIIL 377
              V L P+S                 +E   + N    P RGED+T+ QA+   D  ++
Sbjct: 312 -KDVDLLPASERHKRIAHLQAQLMQAAAEGAVRVNRRRGPHRGEDFTLTQALVKADFWLI 371

Query: 378 FMATICGVGGTLTAIDNLGQIGESLGYQSHSITTFISLVSIWNYLGRVVSGFVSEYFWKK 437
           F + + G G  LT IDNLGQ+ +SLGY +  +   +S++SIWN+LGR+  G+ SE   + 
Sbjct: 372 FFSLLLGSGSGLTVIDNLGQMSQSLGYDNTHV--LVSMISIWNFLGRIGGGYFSELVVRD 431

Query: 438 YKVPRPLFLFATLILSCVGHLLIAFGVPNSLYFSSIVIGFCFGAQWPLIYAIISEIFGLK 497
           Y  PRP+ +    ++  VGH+  A+G P ++Y  +++IG  +GA W ++ A  SE+FGLK
Sbjct: 432 YAYPRPVAMAVAQLIMSVGHIFFAYGWPGAMYIGTLLIGLGYGAHWAIVPATASELFGLK 491

Query: 498 YYATLSSMSGGASPIGAYILNVRVAGHLYDREAQRQMEATGRRRNIGEDLSCLGVECYRK 557
            +  L +    A+P G+ + +  +A  +YDREA+RQ  A G   +  + L C G  C+  
Sbjct: 492 KFGALYNFLTLANPAGSLVFSGMIASSIYDREAERQ--AHGSVFDPDDALRCNGSICFFL 551

BLAST of Cucsa.306610 vs. TAIR10
Match: AT2G34355.1 (AT2G34355.1 Major facilitator superfamily protein)

HSP 1 Score: 351.3 bits (900), Expect = 1.2e-96
Identity = 202/545 (37.06%), Postives = 312/545 (57.25%), Query Frame = 1

Query: 22  RWFSVLASILIMSVAGATYMFALYSSDIKSSLGYDQTTLNLLSFFKDLGGNVGVISGLTN 81
           +W +  ASI I S +GATY FA+YSS +KSS  YDQ+TL+ +S FKD+GG  G+ISG   
Sbjct: 7   KWVAAAASIWIQSFSGATYTFAIYSSILKSSQSYDQSTLDFVSVFKDIGGTFGIISGFLY 66

Query: 82  EVAPA--------WVVLLIGAVMNLFGYTMIWLAVTKRIPKPQIWHMCLYITIGANSQTF 141
               +        WVV+ +G V    G+  IW +V   I  P +  MCL++ +  +S  F
Sbjct: 67  TAMTSKSRGFGGPWVVVFVGLVQWFVGFFFIWASVVGLIAPPPVPLMCLFVFLAGHSLPF 126

Query: 142 ANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLYRAFYGN--NPESLILLIAWLP 201
            NT  +VT  +NF +  G+ +G+++GF+GLSGAIL QLY A  G   NP + ILL+A +P
Sbjct: 127 FNTANVVTAARNFSQYGGTAVGIMQGFLGLSGAILIQLYHAVCGGEGNPATFILLLAIVP 186

Query: 202 AAVSVVLLRFVRIIKDLRQPNELKVFYHFLYISLGLAGTLMVLIILQSLLRFQQIQYVGS 261
             V  + + FVR+ + +   ++ K       IS+ +A  LMV+I ++++L   +   + S
Sbjct: 187 TLVMFLAMPFVRVYETVTISDK-KHLDGLSAISMIIAAYLMVVITVENVLGLSRSMQIFS 246

Query: 262 AIVVIVLLLLPLTIVFR--EELSVWKSKIASPVLQLESASQQPPPPLTSTVSLAPSSPPS 321
            I+V++LL  PL +  R   E     S +  PVL                   A   PPS
Sbjct: 247 FILVLLLLASPLLVAVRALREKRQTLSSLDGPVLDTS----------------ALLDPPS 306

Query: 322 ESCFKNMFNPPSRGEDYTIPQAIFSMDLIILFMATICGVGGTLTAIDNLGQIGESLGYQS 381
            + F +        ED  I +A+ +++  +LF+A +CG+G     ++N+ QIGESL Y S
Sbjct: 307 SNIFPD--GDHLVAEDSNILEAMSTVNFWLLFLAMLCGMGSGFATVNNMRQIGESLRYSS 366

Query: 382 HSITTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGHLLIAFGVPN 441
             + + +SL SIWN+LGR  +G+VS+ F  K+  PRP+F+  TL +  +GH+++A GV  
Sbjct: 367 VQLNSLVSLWSIWNFLGRFGAGYVSDTFLHKHSWPRPIFMAITLGVMAIGHIIVASGVQG 426

Query: 442 SLYFSSIVIGFCFGAQWPLIYAIISEIFGLKYYATLSSMSGGASPIGAYILNVRVAGHLY 501
           SLY  S++IG  +G+QW L+  I SEIFG+++  T+      A PIG+YIL+V+V G+ Y
Sbjct: 427 SLYAGSVLIGMAYGSQWSLMPTITSEIFGIRHMGTIYFTISIAGPIGSYILSVKVIGYFY 486

Query: 502 DREAQRQMEATGRRRNIGEDLSCLGVECYRKAFLIITAATVFGALVSLILVVRTWKFYKG 555
           D+ A              +D SC G +C+R +F+I+ +  +FG+LV+ +L  RT KFYK 
Sbjct: 487 DKVASE------------DDNSCFGSQCFRTSFMIMASVALFGSLVASVLFFRTHKFYKN 520

BLAST of Cucsa.306610 vs. TAIR10
Match: AT1G18940.1 (AT1G18940.1 Nodulin-like / Major Facilitator Superfamily protein)

HSP 1 Score: 345.9 bits (886), Expect = 4.8e-95
Identity = 201/546 (36.81%), Postives = 309/546 (56.59%), Query Frame = 1

Query: 17  QFLFGRWFSVLASILIMSVAGATYMFALYSSDIKSSLGYDQTTLNLLSFFKDLGGNVGVI 76
           + L  +W ++ ASI I   AG +Y F +YS+ +KS+  YDQ+TL+ +S FKD+GGNVGV+
Sbjct: 4   EILRTKWMAMTASIWIQCSAGGSYTFGIYSAILKSTQSYDQSTLDTVSVFKDIGGNVGVL 63

Query: 77  SGLTNEVAP--------------AWVVLLIGAVMNLFGYTMIWLAVTKRIPKPQIWHMCL 136
           SGL    A                WVV+LIGA++N  GY ++W +VT  I +P +  MCL
Sbjct: 64  SGLVYTAATFNRRRRDGRERRGGPWVVILIGAILNFTGYFLMWASVTGLIKRPPVPVMCL 123

Query: 137 YITIGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLYRAFYGNNPES 196
           ++ I A S TF NT  +V+ ++NF +  G+ +G++KGFVGLSGA+L QLY      +P++
Sbjct: 124 FMFIAAQSLTFLNTANVVSSLENFADYGGTAVGIMKGFVGLSGAMLIQLYEVVCPGDPKT 183

Query: 197 LILLIAWLPAAVSVVLLRFVRIIKDLRQPNELKVFYHFLYISLGLAGTLMVLIILQSLLR 256
            ILL+A +P+ +SV+++  VR+ K     +E K       +SL +A  LM+ IIL+S L 
Sbjct: 184 FILLLAIVPSLLSVLVMPLVRVYKT-STVDEKKHLDGLSTLSLIIAAYLMITIILKSTLS 243

Query: 257 FQQIQYVGSAIVVIVLLLLPLTIVFREELSVWKSKIASPVLQLESASQQPPPPLTSTVSL 316
                   +  V++VLL  PL +  R      +  I  P+  + S       PL   +  
Sbjct: 244 LPSWANAVTLAVLLVLLSSPLLVAVRAH----RDSIEKPLSSVYS-------PLVDNLEA 303

Query: 317 APSSPPSESCFKNMFNPPSRGEDYTIPQAIFSMDLIILFMATICGVGGTLTAIDNLGQIG 376
             S                  +   + QA+ ++D  +LF+A ICG+G  ++ I+N+ QIG
Sbjct: 304 TTSGE---------ILMLDEDKSLNLLQAMCNVDFWLLFLAMICGMGSGISTINNIRQIG 363

Query: 377 ESLGYQSHSITTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGHLL 436
           ESL Y S  I + ++L +IWN++GR   G+VS++   +   PRPL +  TL    +GHL+
Sbjct: 364 ESLRYTSVEINSLLALWNIWNFIGRFGGGYVSDWLLHRKGWPRPLLMATTLGTMTIGHLI 423

Query: 437 IAFGVPNSLYFSSIVIGFCFGAQWPLIYAIISEIFGLKYYATLSSMSGGASPIGAYILNV 496
           IA G   +LY  SI++G C+G+QW L+  I SE+FG+K+  T+ +    ASP+G+YI +V
Sbjct: 424 IASGFQGNLYPGSIIVGICYGSQWSLMPTITSELFGVKHMGTIYNTISIASPMGSYIFSV 483

Query: 497 RVAGHLYDREAQRQMEATGRRRNIGEDLSCLGVECYRKAFLIITAATVFGALVSLILVVR 549
           R+ G++YDR              IGE  +C G  C+R A+++I +    G LVS +LV R
Sbjct: 484 RLIGYIYDRTI------------IGEGNTCYGPHCFRLAYVVIASVAFLGFLVSCVLVFR 516

BLAST of Cucsa.306610 vs. NCBI nr
Match: gi|778683937|ref|XP_011651970.1| (PREDICTED: uncharacterized protein LOC101216551 [Cucumis sativus])

HSP 1 Score: 1146.0 bits (2963), Expect = 0.0e+00
Identity = 580/580 (100.00%), Postives = 580/580 (100.00%), Query Frame = 1

Query: 1   MAASSSNPTSSNPFWYQFLFGRWFSVLASILIMSVAGATYMFALYSSDIKSSLGYDQTTL 60
           MAASSSNPTSSNPFWYQFLFGRWFSVLASILIMSVAGATYMFALYSSDIKSSLGYDQTTL
Sbjct: 1   MAASSSNPTSSNPFWYQFLFGRWFSVLASILIMSVAGATYMFALYSSDIKSSLGYDQTTL 60

Query: 61  NLLSFFKDLGGNVGVISGLTNEVAPAWVVLLIGAVMNLFGYTMIWLAVTKRIPKPQIWHM 120
           NLLSFFKDLGGNVGVISGLTNEVAPAWVVLLIGAVMNLFGYTMIWLAVTKRIPKPQIWHM
Sbjct: 61  NLLSFFKDLGGNVGVISGLTNEVAPAWVVLLIGAVMNLFGYTMIWLAVTKRIPKPQIWHM 120

Query: 121 CLYITIGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLYRAFYGNNP 180
           CLYITIGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLYRAFYGNNP
Sbjct: 121 CLYITIGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLYRAFYGNNP 180

Query: 181 ESLILLIAWLPAAVSVVLLRFVRIIKDLRQPNELKVFYHFLYISLGLAGTLMVLIILQSL 240
           ESLILLIAWLPAAVSVVLLRFVRIIKDLRQPNELKVFYHFLYISLGLAGTLMVLIILQSL
Sbjct: 181 ESLILLIAWLPAAVSVVLLRFVRIIKDLRQPNELKVFYHFLYISLGLAGTLMVLIILQSL 240

Query: 241 LRFQQIQYVGSAIVVIVLLLLPLTIVFREELSVWKSKIASPVLQLESASQQPPPPLTSTV 300
           LRFQQIQYVGSAIVVIVLLLLPLTIVFREELSVWKSKIASPVLQLESASQQPPPPLTSTV
Sbjct: 241 LRFQQIQYVGSAIVVIVLLLLPLTIVFREELSVWKSKIASPVLQLESASQQPPPPLTSTV 300

Query: 301 SLAPSSPPSESCFKNMFNPPSRGEDYTIPQAIFSMDLIILFMATICGVGGTLTAIDNLGQ 360
           SLAPSSPPSESCFKNMFNPPSRGEDYTIPQAIFSMDLIILFMATICGVGGTLTAIDNLGQ
Sbjct: 301 SLAPSSPPSESCFKNMFNPPSRGEDYTIPQAIFSMDLIILFMATICGVGGTLTAIDNLGQ 360

Query: 361 IGESLGYQSHSITTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGH 420
           IGESLGYQSHSITTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGH
Sbjct: 361 IGESLGYQSHSITTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGH 420

Query: 421 LLIAFGVPNSLYFSSIVIGFCFGAQWPLIYAIISEIFGLKYYATLSSMSGGASPIGAYIL 480
           LLIAFGVPNSLYFSSIVIGFCFGAQWPLIYAIISEIFGLKYYATLSSMSGGASPIGAYIL
Sbjct: 421 LLIAFGVPNSLYFSSIVIGFCFGAQWPLIYAIISEIFGLKYYATLSSMSGGASPIGAYIL 480

Query: 481 NVRVAGHLYDREAQRQMEATGRRRNIGEDLSCLGVECYRKAFLIITAATVFGALVSLILV 540
           NVRVAGHLYDREAQRQMEATGRRRNIGEDLSCLGVECYRKAFLIITAATVFGALVSLILV
Sbjct: 481 NVRVAGHLYDREAQRQMEATGRRRNIGEDLSCLGVECYRKAFLIITAATVFGALVSLILV 540

Query: 541 VRTWKFYKGDIYRKFREVEGEDIEMKMAAPTNGTLTAAKN 581
           VRTWKFYKGDIYRKFREVEGEDIEMKMAAPTNGTLTAAKN
Sbjct: 541 VRTWKFYKGDIYRKFREVEGEDIEMKMAAPTNGTLTAAKN 580

BLAST of Cucsa.306610 vs. NCBI nr
Match: gi|659084118|ref|XP_008442718.1| (PREDICTED: LOW QUALITY PROTEIN: uncharacterized protein LOC103486508 [Cucumis melo])

HSP 1 Score: 857.4 bits (2214), Expect = 1.4e-245
Identity = 436/536 (81.34%), Postives = 465/536 (86.75%), Query Frame = 1

Query: 9   TSSNPFWYQFLFGRWFSVLASILIMSVAGATYMFALYSSDIKSSLGYDQTTLNLLSFFKD 68
           TS NPFW+Q  FGRWFS  ASIL+MSV+GA +MFAL SSDIKSS GYDQTT NLLSFFK 
Sbjct: 3   TSGNPFWHQLFFGRWFSAFASILMMSVSGAAFMFALSSSDIKSSFGYDQTTXNLLSFFKG 62

Query: 69  LGGNVGVISGLTNEVAPAWVVLLIGAVMNLFGYTMIWLAVTKRIPKPQIWHMCLYITIGA 128
           LGGN GVISGL NEVAP W +LLIGAVMNLFGYTMIWLAVTK IP PQIWHMCLYI IGA
Sbjct: 63  LGGNAGVISGLINEVAPTWRILLIGAVMNLFGYTMIWLAVTKPIPNPQIWHMCLYICIGA 122

Query: 129 NSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLYRAFYGNNPESLILLIA 188
           NSQTFANTGA+VTCV NFPESRGSVLGLLKG+VGLSGAILSQLY AFYGNN +S ILLIA
Sbjct: 123 NSQTFANTGAIVTCVNNFPESRGSVLGLLKGYVGLSGAILSQLYHAFYGNNSKSFILLIA 182

Query: 189 WLPAAVSVVLLRFVRIIKDLRQPNELKVFYHFLYISLGLAGTLMVLIILQSLLRFQQIQY 248
           WLPAAV VV LRFVRIIKDL QPNE+KVFY  LYISLGLAG+L V IILQ+ +RFQQIQY
Sbjct: 183 WLPAAVIVVFLRFVRIIKDLLQPNEVKVFYRILYISLGLAGSLTVFIILQNRIRFQQIQY 242

Query: 249 VGSAIVVIVLLLLPL-TIVFREELSVWKSKIASPVLQLESASQQPPPPLTSTVSLAPSSP 308
           VGSAIVVIVLLLLPL  IVFREEL VWK KIA+P+ QLE ASQQPPP     +S  P SP
Sbjct: 243 VGSAIVVIVLLLLPLAAIVFREELIVWKRKIANPISQLELASQQPPP----RISAVPLSP 302

Query: 309 PSESCFKNMFNPPSRGEDYTIPQAIFSMDLIILFMATICGVGGTLTAIDNLGQIGESLGY 368
           PS+SCFKNMF PP+RGEDYTIPQAIFS+D+ ILFMATIC VGGTLTA+DNLGQIGES GY
Sbjct: 303 PSDSCFKNMFKPPNRGEDYTIPQAIFSVDMKILFMATICAVGGTLTAMDNLGQIGESSGY 362

Query: 369 QSHSITTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPR-PLFLFATLILSCVGHLLIAFG 428
            S S TTFISLVSIWNYLGRVVSGFVSEY WKK+KVPR PLFLF TLI+SCVGHLLIAFG
Sbjct: 363 PSQSTTTFISLVSIWNYLGRVVSGFVSEYLWKKFKVPRPPLFLFGTLIISCVGHLLIAFG 422

Query: 429 VPNSLYFSSIVIGFCFGAQWPLIYAIISEIFGLKYYATLSSMSGGASPIGAYILNVRVAG 488
           VPNSLYFS I+IGFCFGAQ PLI AI SEIFGLKYYATLS+    A+PIG YI+NV+V G
Sbjct: 423 VPNSLYFSPIIIGFCFGAQLPLILAITSEIFGLKYYATLSNFGVAANPIGTYIMNVKVVG 482

Query: 489 HLYDREAQRQMEATGRRRNIGEDLSCLGVECYRKAFLIITAATVFGALVSLILVVR 543
           HLYDREA+RQMEA G RR IGE LSCLGVECYRKAFLIIT  TV G +VSLILVVR
Sbjct: 483 HLYDREAERQMEAAGGRRKIGESLSCLGVECYRKAFLIITGTTVLGGVVSLILVVR 534

BLAST of Cucsa.306610 vs. NCBI nr
Match: gi|359481931|ref|XP_002268663.2| (PREDICTED: uncharacterized protein LOC100248651 [Vitis vinifera])

HSP 1 Score: 770.4 bits (1988), Expect = 2.3e-219
Identity = 387/575 (67.30%), Postives = 459/575 (79.83%), Query Frame = 1

Query: 14  FWYQFLFGRWFSVLASILIMSVAGATYMFALYSSDIKSSLGYDQTTLNLLSFFKDLGGNV 73
           F  Q L GRWF V AS+LIMSVAGATYMF+LYS +IK+SLGYDQTTLNLLSFFKDLGGNV
Sbjct: 18  FGLQVLVGRWFMVFASLLIMSVAGATYMFSLYSGEIKTSLGYDQTTLNLLSFFKDLGGNV 77

Query: 74  GVISGLTNEVAPAWVVLLIGAVMNLFGYTMIWLAVTKRIPKPQIWHMCLYITIGANSQTF 133
           GV+SGL NEV P WVVL IGAVMN FGY MIWLAVT RI KPQIW MCLYI IGANSQ+F
Sbjct: 78  GVLSGLINEVTPPWVVLCIGAVMNFFGYFMIWLAVTDRIAKPQIWQMCLYICIGANSQSF 137

Query: 134 ANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLYRAFYGNNPESLILLIAWLPAA 193
           ANTGALVTCV+NFPESRG VLGLLKGFVGLSGAI++QLYRAFYG++ +SLIL IAWLPAA
Sbjct: 138 ANTGALVTCVRNFPESRGIVLGLLKGFVGLSGAIITQLYRAFYGDDSKSLILFIAWLPAA 197

Query: 194 VSVVLLRFVRIIKDLRQPNELKVFYHFLYISLGLAGTLMVLIILQSLLRFQQIQYVGSAI 253
           VS V LR +RI+K  RQ NELKVFY  LY+SLGLAG LMV+II+Q+   F +I+Y GSA 
Sbjct: 198 VSFVFLRTIRIMKVGRQANELKVFYDLLYMSLGLAGFLMVIIIIQNKFTFSRIEYSGSAA 257

Query: 254 VVIVLLLLPLTIVFREELSVWK-SKIASPVLQLESASQQPP------PPLTSTVSLAP-- 313
           VV++LL LPL +V +EE+++WK  K A    Q++  ++ PP       P+ S   L P  
Sbjct: 258 VVLILLFLPLAVVIKEEINIWKGKKQALDAAQVKVITENPPAVELASSPVVSLDQLPPPT 317

Query: 314 ----SSPPSESCFKNMFNPPSRGEDYTIPQAIFSMDLIILFMATICGVGGTLTAIDNLGQ 373
               ++  S SCFK MF PP RGEDYTI QA+FS+D++ILF+ T CGVGGTLTAIDNLGQ
Sbjct: 318 AAPENAEKSVSCFKTMFKPPDRGEDYTILQALFSIDMLILFIVTTCGVGGTLTAIDNLGQ 377

Query: 374 IGESLGYQSHSITTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGH 433
           IG S GY +HS TTF+SLVSIWNYLGRVVSGF SE F  +YK PRPL L   L+ SCVGH
Sbjct: 378 IGSSQGYTAHSTTTFVSLVSIWNYLGRVVSGFASEIFLTRYKFPRPLMLTFVLLFSCVGH 437

Query: 434 LLIAFGVPNSLYFSSIVIGFCFGAQWPLIYAIISEIFGLKYYATLSSMSGGASPIGAYIL 493
           LLIAF VPNSLYF+S++IGFCFGAQWPL++AIISE+FGLKYY+TL +    ASP+G+YIL
Sbjct: 438 LLIAFAVPNSLYFASVIIGFCFGAQWPLVFAIISELFGLKYYSTLYNFGAVASPVGSYIL 497

Query: 494 NVRVAGHLYDREAQRQMEATGRRRNIGEDLSCLGVECYRKAFLIITAATVFGALVSLILV 553
           NV+VAGHLYD+EA +Q+EA+G  R  G+DL+C G  CY+ +F+IITAAT+FG ++S ILV
Sbjct: 498 NVKVAGHLYDKEALKQLEASGVTRVAGQDLTCTGAACYKLSFIIITAATLFGCIISFILV 557

Query: 554 VRTWKFYKGDIYRKFREVEGEDIEMKMAAPTNGTL 576
           +RT KFY+GDIY+KFR+ E    E+ MA+  NG L
Sbjct: 558 IRTKKFYQGDIYKKFRQ-EANKAEINMASSGNGIL 591

BLAST of Cucsa.306610 vs. NCBI nr
Match: gi|802687698|ref|XP_012082447.1| (PREDICTED: protein NUCLEAR FUSION DEFECTIVE 4-like [Jatropha curcas])

HSP 1 Score: 762.7 bits (1968), Expect = 4.7e-217
Identity = 376/548 (68.61%), Postives = 452/548 (82.48%), Query Frame = 1

Query: 14  FWYQFLFGRWFSVLASILIMSVAGATYMFALYSSDIKSSLGYDQTTLNLLSFFKDLGGNV 73
           F+ + + GRWF V ASILIM+VAGATYMF LYS+DIK+SLGYDQTTLNLLSFFKDLGGNV
Sbjct: 19  FFLKMILGRWFMVFASILIMAVAGATYMFGLYSNDIKTSLGYDQTTLNLLSFFKDLGGNV 78

Query: 74  GVISGLTNEVAPAWVVLLIGAVMNLFGYTMIWLAVTKRIPKPQIWHMCLYITIGANSQTF 133
           GV+SGL NEV P WVVL IGA+MN FGY MIWLAVT  IPKP++W MCLYI IGANSQ F
Sbjct: 79  GVLSGLINEVTPPWVVLSIGAIMNFFGYFMIWLAVTGHIPKPKVWQMCLYICIGANSQAF 138

Query: 134 ANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLYRAFYGNNPESLILLIAWLPAA 193
           ANTGALVTCVKNFPESRG+VLGLLK FVGLSGAI +Q+Y A YG + +SLILLIAWLPAA
Sbjct: 139 ANTGALVTCVKNFPESRGAVLGLLKSFVGLSGAIFTQIYHALYGYDSKSLILLIAWLPAA 198

Query: 194 VSVVLLRFVRIIKDLRQPNELKVFYHFLYISLGLAGTLMVLIILQSLLRFQQIQYVGSAI 253
           +S + LR +RII+ +RQ NELK+FY  LYISLGLAG LMVLII+Q+   F +I+Y+GS+ 
Sbjct: 199 ISFIFLRTIRIIEVVRQANELKIFYKLLYISLGLAGFLMVLIIMQNKFNFTRIEYIGSSS 258

Query: 254 VVIVLLLLPLTIVFREELSVWKSKIASPVLQ--LESASQQPPPPLTSTVSLAPSSPP--S 313
           +V++LL LP+ +V +EE  V KSK A+   Q  ++  +Q+ P    S+V ++ SSPP   
Sbjct: 259 IVLLLLFLPIVVVIKEEYHVRKSKKAALANQSSIKVITQENP----SSVEVSSSSPPRAQ 318

Query: 314 ESCFKNMFNPPSRGEDYTIPQAIFSMDLIILFMATICGVGGTLTAIDNLGQIGESLGYQS 373
            SC  N+FNPP+RGEDYTI QA+FS+D++ILF+AT CGVGGTLTA+DNLGQIG SLGY S
Sbjct: 319 PSCTNNIFNPPNRGEDYTILQALFSVDMLILFIATTCGVGGTLTAVDNLGQIGNSLGYPS 378

Query: 374 HSITTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGHLLIAFGVPN 433
           HS TTF+SLVSIWNYLGR V+GF SE+   KYK+PRPL     L+ SCVGHLLIAFGVPN
Sbjct: 379 HSTTTFVSLVSIWNYLGRAVAGFASEFLLTKYKIPRPLLFTLVLLFSCVGHLLIAFGVPN 438

Query: 434 SLYFSSIVIGFCFGAQWPLIYAIISEIFGLKYYATLSSMSGGASPIGAYILNVRVAGHLY 493
           SLYF+S++IGFCFGAQWPL++AIISEIFGLKYY+TL +    ASP+G+YILNVRVAGHLY
Sbjct: 439 SLYFASVIIGFCFGAQWPLLFAIISEIFGLKYYSTLYNFGSVASPVGSYILNVRVAGHLY 498

Query: 494 DREAQRQMEATGRRRNIGEDLSCLGVECYRKAFLIITAATVFGALVSLILVVRTWKFYKG 553
           D+EA +Q+E  G +R  G+ L+C GV+CY+ AFLIITAAT+FG ++S ILVVRT KFY+G
Sbjct: 499 DKEALKQIEGLGLKREAGKYLTCSGVQCYKMAFLIITAATLFGCVISSILVVRTRKFYQG 558

Query: 554 DIYRKFRE 558
           DIY+KFRE
Sbjct: 559 DIYKKFRE 562

BLAST of Cucsa.306610 vs. NCBI nr
Match: gi|590631034|ref|XP_007027453.1| (Major facilitator superfamily protein [Theobroma cacao])

HSP 1 Score: 760.0 bits (1961), Expect = 3.0e-216
Identity = 393/594 (66.16%), Postives = 457/594 (76.94%), Query Frame = 1

Query: 14  FWYQFLFGRWFSVLASILIMSVAGATYMFALYSSDIKSSLGYDQTTLNLLSFFKDLGGNV 73
           F    + GRWF V AS+LIMSV+GATYMF LYS+ IKSSLGYDQTTLNLLSF KDLGGNV
Sbjct: 19  FGLNLIIGRWFMVFASLLIMSVSGATYMFGLYSTVIKSSLGYDQTTLNLLSFSKDLGGNV 78

Query: 74  GVISGLTNEVAPAWVVLLIGAVMNLFGYTMIWLAVTKRIPKPQIWHMCLYITIGANSQTF 133
           GV+SGL NEV P WVVLLIG+VMN FGY MIWLAVT R  KP +W MC+YI +GANSQ F
Sbjct: 79  GVLSGLINEVTPPWVVLLIGSVMNFFGYFMIWLAVTGRTAKPHVWQMCVYICLGANSQAF 138

Query: 134 ANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLYRAFYGNNPESLILLIAWLPAA 193
           ANTGALVTCVKNFPESRGSVLGLLK +VGLSGAIL+QLY AFYG N +SLILLIAWLPAA
Sbjct: 139 ANTGALVTCVKNFPESRGSVLGLLKSYVGLSGAILTQLYHAFYGENSKSLILLIAWLPAA 198

Query: 194 VSVVLLRFVRIIKDLRQPNELKVFYHFLYISLGLAGTLMVLIILQSLLRFQQIQYVGSAI 253
           VS V LR +RIIK +RQ NEL+VFY+  YISL LAG LMVLI++Q+ L F +I+YVGSA 
Sbjct: 199 VSFVFLRTIRIIKIVRQTNELRVFYNMFYISLALAGFLMVLIVIQNRLSFNRIEYVGSAS 258

Query: 254 VVIVLLLLPLTIVFREELSVW--KSKIASPVLQLESASQQPPPPLTSTVSLAPSSPPSES 313
           +V +LLL PLT+V RE+  +W  K +  + V Q++  ++ PP     TV LAP +   ES
Sbjct: 259 LVTLLLLFPLTVVVREDFKIWRRKKQALNDVSQMKVVTENPP-----TVELAPQTMAVES 318

Query: 314 ------------------CFKNMFNPPSRGEDYTIPQAIFSMDLIILFMATICGVGGTLT 373
                             C +N+F PP RGEDYTI QA+FS+D++ILF+AT CGVGGTLT
Sbjct: 319 LQPQPAGDPKSPDQKHVACMENIFKPPDRGEDYTILQALFSIDMLILFIATTCGVGGTLT 378

Query: 374 AIDNLGQIGESLGYQSHSITTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATL 433
           AIDNLGQIG SLGY S SITTF+SLVSIWNYLGR V+GF SEY   KYK+PRPL   + +
Sbjct: 379 AIDNLGQIGNSLGYPSRSITTFVSLVSIWNYLGRAVAGFFSEYLLTKYKIPRPLLFTSVI 438

Query: 434 ILSCVGHLLIAFGVPNSLYFSSIVIGFCFGAQWPLIYAIISEIFGLKYYATLSSMSGGAS 493
           + SCVGHLLIAF VPNSLYF+S++IGFCFGAQWPL+YAIISEIFGLKYY+TL +    AS
Sbjct: 439 LFSCVGHLLIAFAVPNSLYFASVIIGFCFGAQWPLMYAIISEIFGLKYYSTLYNFGAVAS 498

Query: 494 PIGAYILNVRVAGHLYDREAQRQMEATGRRRNIGEDLSCLGVECYRKAFLIITAATVFGA 553
           P+G+YILNVRVAGHLYD+EA +QM+A GR R  GEDL+C G +CYR AF+II A T+FG 
Sbjct: 499 PVGSYILNVRVAGHLYDKEALKQMKALGRTRKPGEDLTCTGGQCYRMAFIIIIATTLFGF 558

Query: 554 LVSLILVVRTWKFYKGDIYRKFREVEGEDIEMKMA---------APTN-GTLTA 578
           LVS ILV+RT KFYK DIY+KFRE E E  E  MA         APT+ GT TA
Sbjct: 559 LVSCILVLRTRKFYKSDIYKKFRE-EAEAAETDMASTSKQVEPKAPTDAGTTTA 606