Cucsa.303020 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.303020 vs. Swiss-Prot
Match: 4CLLA_ARATH (Oxalate--CoA ligase OS=Arabidopsis thaliana GN=AAE3 PE=1 SV=1)

HSP 1 Score: 773.5 bits (1996), Expect = 1.5e-222
Identity = 390/511 (76.32%), Postives = 438/511 (85.71%), Query Frame = 1

Query: 1   MENCTFTGLLKKAASEFPRCRALSVSGKFDLTHERLQELIEHAAARLV-DAGVKAGDVVA 60
           M++ T +GLL+  A +FP  RALSVSGKF+LTH RL +LIE AA+RLV DAG+K GDVVA
Sbjct: 1   MDSDTLSGLLENVAKKFPDRRALSVSGKFNLTHARLHDLIERAASRLVSDAGIKPGDVVA 60

Query: 61  LTFPNTVEYVIMFLAVIRCRATAAPLNSAYTAEEFEFYLSDSESKLLLTSKEGISSAQTA 120
           LTFPNTVE+VIMFLAVIR RATAAPLN+AYTAEEFEFYLSDS+SKLLLTSKEG + AQ A
Sbjct: 61  LTFPNTVEFVIMFLAVIRARATAAPLNAAYTAEEFEFYLSDSDSKLLLTSKEGNAPAQEA 120

Query: 121 ATKLNIPQVKANL-SSGDEFIELFPSPIETGSNVVEIVNDPSDAALFLHTSGTTSRPKGV 180
           A+KL I  V A L  +G + +           +  E+VN P D ALFLHTSGTTSRPKGV
Sbjct: 121 ASKLKISHVTATLLDAGSDLVLSVADSDSVVDSATELVNHPDDGALFLHTSGTTSRPKGV 180

Query: 181 PLTQQNLGASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLIAGAAVTLPAAGRF 240
           PLTQ NL +SV+NIK+VYKL+ESDSTV+VLPLFHVHGL+AGLLSSL AGAAVTLPAAGRF
Sbjct: 181 PLTQLNLASSVKNIKAVYKLTESDSTVIVLPLFHVHGLLAGLLSSLGAGAAVTLPAAGRF 240

Query: 241 SASTFWSDMIAYNATWYTAVPTIHQIILERHLSKPEPSYPKLRFIRSCSASLAPSILERL 300
           SA+TFW DM  YNATWYTAVPTIHQIIL+RH S PE  YPKLRFIRSCSASLAP IL RL
Sbjct: 241 SATTFWPDMKKYNATWYTAVPTIHQIILDRHASHPETEYPKLRFIRSCSASLAPVILSRL 300

Query: 301 EQSFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENGAIQSEGVK 360
           E++FGAPVLE+YAMTEA+HLM+SNPLPE+G HK GSVGKP+GQEMAIL+E G IQ    K
Sbjct: 301 EEAFGAPVLEAYAMTEATHLMSSNPLPEEGPHKPGSVGKPVGQEMAILNEKGEIQEPNNK 360

Query: 361 GEVCIRGPNVTKGYKNNPDANNSAFMFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEK 420
           GEVCIRGPNVTKGYKNNP+AN + F FGWFHTGDIG+FD+DGYLHLVGRIKELINRGGEK
Sbjct: 361 GEVCIRGPNVTKGYKNNPEANKAGFEFGWFHTGDIGYFDTDGYLHLVGRIKELINRGGEK 420

Query: 421 ISPIEVDAVLLSHTDVSQAVAFGVPDNKYGEEINCAIIPREGSSINEADVLQFCKKNLAS 480
           ISPIEVDAVLL+H DVSQ VAFGVPD KYGEEINCA+IPREG+++ E D+  FCKKNLA+
Sbjct: 421 ISPIEVDAVLLTHPDVSQGVAFGVPDEKYGEEINCAVIPREGTTVTEEDIKAFCKKNLAA 480

Query: 481 FKVPKKVFITDYLPKTASGKIQRRFVAEHFL 510
           FKVPK+VFITD LPKTASGKIQRR VA+HFL
Sbjct: 481 FKVPKRVFITDNLPKTASGKIQRRIVAQHFL 511

BLAST of Cucsa.303020 vs. Swiss-Prot
Match: FAT2_SCHPO (Putative peroxisomal-coenzyme A synthetase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=SPCC1827.03c PE=1 SV=1)

HSP 1 Score: 442.2 bits (1136), Expect = 7.8e-123
Identity = 242/520 (46.54%), Postives = 321/520 (61.73%), Query Frame = 1

Query: 5   TFTGLLKKAASEFPRCRALSVSGKFDLTHERLQELIEHAAARLVDAGVKAGDVVALTFPN 64
           +FT L      +      ++ S   +L+   L+  I     ++   G+K GD V +  PN
Sbjct: 2   SFTTLYSAIQGDASARALVAPSLNAELSFSELRIAIMDLQRQIASLGIKVGDPVNIAIPN 61

Query: 65  TVEYVIMFLAVIRCRATAAPLNSAYTAEEFEFYLSDSESKLLLTSKEGISSAQTAATKLN 124
            +E+V+ F AV   RA   PLNS Y   EFEFY+ D +SKL++  +  +++        N
Sbjct: 62  GLEFVVAFYAVSWQRAICGPLNSNYKQSEFEFYIDDLKSKLVIVPEGSVAA--------N 121

Query: 125 IPQVKANLSSGDEFIELFPSPIETGSNVVEIVN---------------DPSDAALFLHTS 184
            P V+A         EL   P    S +V IV+                P D  L LHTS
Sbjct: 122 TPAVRAAKKLSVAVAELAWCP---KSRLVRIVHFEGAKINAPQPLGLPQPDDVMLVLHTS 181

Query: 185 GTTSRPKGVPLTQQNLGASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLIAGAA 244
           GTT RPK VPLT +NL  S+ NI + Y+L   D++ +V+PLFHVHGL+ GLLS+L +G  
Sbjct: 182 GTTGRPKVVPLTHKNLCRSIHNITTSYRLDPRDTSYVVMPLFHVHGLLCGLLSTLASGGC 241

Query: 245 VTLPAAGRFSASTFWSDMIAYNATWYTAVPTIHQIILERHLSKPEPSYPKLRFIRSCSAS 304
             +P   +FSA +FW + I Y ATWYTAVPTIHQI+L     KP    P++RFIRSCS+ 
Sbjct: 242 AVVPP--KFSAHSFWKEFIQYGATWYTAVPTIHQILLRTPPPKP---LPRIRFIRSCSSP 301

Query: 305 LAPSILERLEQSFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDEN 364
           LAP +L +LE +F APVLE+YAMTEASH M +NPLP   VHK  SVGKP G E+ ILD+ 
Sbjct: 302 LAPPVLSKLEATFRAPVLEAYAMTEASHQMTTNPLPPL-VHKPHSVGKPFGVELKILDQK 361

Query: 365 GAIQSEGVKGEVCIRGPNVTKGYKNNPDANNSAFMFG-WFHTGDIGFFDSDGYLHLVGRI 424
           G    +G +GE+C+RG NVTKGY NNP AN S+F    +F TGD G  D DGY+ + GRI
Sbjct: 362 GNEMPQGKEGEICVRGINVTKGYLNNPAANKSSFTKDRFFRTGDEGKLDKDGYVFITGRI 421

Query: 425 KELINRGGEKISPIEVDAVLLSHTDVSQAVAFGVPDNKYGEEINCAIIPREGSSINEADV 484
           KEL+NRGGEKISP E+DAVL+ H DVS+AV F VPD KYG++I  AI P  G ++    +
Sbjct: 422 KELVNRGGEKISPAEIDAVLMQHPDVSEAVCFAVPDEKYGQDIQAAINPVAGKTVTPKQL 481

Query: 485 LQFCKKNLASFKVPKKVFITDYLPKTASGKIQRRFVAEHF 509
             + ++ +A+FK+PKK + TD +PKTA+GK+QRR V + F
Sbjct: 482 HDYLEQKVAAFKIPKKFYFTDRIPKTATGKVQRRLVCDAF 504

BLAST of Cucsa.303020 vs. Swiss-Prot
Match: FAT2_YEAST (Peroxisomal-coenzyme A synthetase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PCS60 PE=1 SV=1)

HSP 1 Score: 382.9 bits (982), Expect = 5.6e-105
Identity = 217/485 (44.74%), Postives = 287/485 (59.18%), Query Frame = 1

Query: 56  DVVALTFPNTVEYVIMFLAVIRCRATAAPLNSAYTAEEFEFYLSDSESKLLLTSKEGISS 115
           D VA++  N +E+++ FL         APLN  Y  +EF FYL+D +SK +   K     
Sbjct: 63  DTVAISMRNGLEFIVAFLGATMDAKIGAPLNPNYKEKEFNFYLNDLKSKAICVPK----- 122

Query: 116 AQTAATKLNIPQVKANLSSGDEFI--------------------ELFPSPIETGSNVVEI 175
                TKL   ++  + S+   FI                    + +   I    N  + 
Sbjct: 123 ---GTTKLQSSEILKSASTFGCFIVELAFDATRFRVEYDIYSPEDNYKRVIYRSLNNAKF 182

Query: 176 VND----------PSDAALFLHTSGTTSRPKGVPLTQQNLGASVQNIKSVYKLSESDSTV 235
           VN            SD AL LHTSGTTS PK VPL   N+  S  NI + YKL+  D + 
Sbjct: 183 VNTNPVKFPGFARSSDVALILHTSGTTSTPKTVPLLHLNIVRSTLNIANTYKLTPLDRSY 242

Query: 236 LVLPLFHVHGLMAGLLSSLIAGAAVTLPAAGRFSASTFWSDMIAYNATWYTAVPTIHQII 295
           +V+PLFHVHGL+  LLS+     +V +P    F    FW   + YN  W++ VPTI  I+
Sbjct: 243 VVMPLFHVHGLIGVLLSTFRTQGSVVVPDG--FHPKLFWDQFVKYNCNWFSCVPTISMIM 302

Query: 296 LERHLSKPEPSYPKLRFIRSCSASLAPSILERLEQSFGAPVLESYAMTEASHLMASNPLP 355
           L  ++ KP P +P +RFIRSCS++LAP+   +LE+ F APVLE+YAMTEASH M SN LP
Sbjct: 303 L--NMPKPNP-FPHIRFIRSCSSALAPATFHKLEKEFNAPVLEAYAMTEASHQMTSNNLP 362

Query: 356 EDGVHKAGSVGKPIGQEMAILDENGAIQSEGVKGEVCIRGPNVTKGYKNNPDANNSAFMF 415
             G  K G+VG+P G  + ILD+N  +   G  GEV IRG NVT GY NNP AN   F  
Sbjct: 363 P-GKRKPGTVGQPQGVTVVILDDNDNVLPPGKVGEVSIRGENVTLGYANNPKANKENFTK 422

Query: 416 --GWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKISPIEVDAVLLSHTDVSQAVAFGVP 475
              +F TGD G+FD +G+L L GRIKELINRGGEKISPIE+D ++LSH  + +AVAFGVP
Sbjct: 423 RENYFRTGDQGYFDPEGFLVLTGRIKELINRGGEKISPIELDGIMLSHPKIDEAVAFGVP 482

Query: 476 DNKYGEEINCAIIPREGSSINEADVLQFCKKNLASFKVPKKVFITDYLPKTASGKIQRRF 509
           D+ YG+ +  AI+ ++G  +   +++ F KK+LASFK+P KV+  D LPKTA+GKIQRR 
Sbjct: 483 DDMYGQVVQAAIVLKKGEKMTYEELVNFLKKHLASFKIPTKVYFVDKLPKTATGKIQRRV 533

BLAST of Cucsa.303020 vs. Swiss-Prot
Match: SAUT_CUPNH (Probable sulfoacetate--CoA ligase OS=Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) GN=sauT PE=2 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 4.8e-72
Identity = 158/460 (34.35%), Postives = 242/460 (52.61%), Query Frame = 1

Query: 50  AGVKAGDVVALTFPNTVEYVIMFLAVIRCRATAAPLNSAYTAEEFEFYLSDSESKLLLTS 109
           AG+ +G  V++  PN  +   + L  + C     P+N      +  + L+ S+++L+ T 
Sbjct: 58  AGLGSGQTVSVYLPNGEQTARLLLGTMACGLVVNPINLLCQPAQLRYILAHSDTRLVFTW 117

Query: 110 KEGISSAQTAATKLNIPQVKANLSSGDEFIELFPSPIETGSNVVEIVNDPSDAALFLHTS 169
            +G ++ + A  +  +       +     +   P+  +  S +      P   AL ++TS
Sbjct: 118 PDGEAAIREALREAGLDVPVLVTAPDANSLPALPATHDAASPLPP--PQPDAPALLMYTS 177

Query: 170 GTTSRPKGVPLTQQNLGASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLIAGAA 229
           GTT  PKGV LTQ+NL A+  N+   + L  +D  +  LPL+H++GL+   ++ L+ G +
Sbjct: 178 GTTGTPKGVLLTQRNLVANGTNVSREHCLGPADRVLATLPLYHINGLVVTAIAPLVHGGS 237

Query: 230 VTLPAAGRFSASTFWSDMIAYNATWYTAVPTIHQIILERHLSKPEPSYPK-LRFIRSCSA 289
           V +P   RFSAS FW D   +  TW   VPTI   +L    + P    P  +RF RS SA
Sbjct: 238 VVMPM--RFSASAFWQDSARHGCTWLNVVPTIIAYLL----NDPHGQAPAGVRFCRSASA 297

Query: 290 SLAPSILERLEQSFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDE 349
           +L P      E  FG  V+E+  MTE +    SNPL + G  + GS+G+P G    +L  
Sbjct: 298 ALPPEHHRAFEARFGIGVIETMGMTETAAPAFSNPL-DPGQRRIGSIGRPSGTRARVLGR 357

Query: 350 NGAIQSEGVKGEVCIRGPNVTKGYKNNPDANNSAFMF-GWFHTGDIGFFDSDGYLHLVGR 409
           +G    +G  GE+ ++G +V  GY   PD    AF   GW  TGD+G+ D+DGY ++ GR
Sbjct: 358 DGKPAPDGQVGEIVLQGESVMAGYYKAPDITREAFTHDGWLRTGDLGYRDADGYFYISGR 417

Query: 410 IKELINRGGEKISPIEVDAVLLSHTDVSQAVAFGVPDNKYGEEINCAIIPREGSSINEAD 469
            KELI +GGE I+P E+D  LL H  V +A A GVPD  YG+EI   ++ RE +  ++A 
Sbjct: 418 AKELIIKGGENIAPREIDEALLRHPGVLEAAAVGVPDPAYGQEIVAYVVMREAARCDDAA 477

Query: 470 VLQFCKKNLASFKVPKKVFITDYLPKTASGKIQRRFVAEH 508
           +   C + L  +K PK+      LP+  SGK+QR  +  H
Sbjct: 478 LRAHCLRELGRYKTPKEFRFIAELPRGPSGKVQRLKLLNH 508

BLAST of Cucsa.303020 vs. Swiss-Prot
Match: LCFB_BACSU (Long-chain-fatty-acid--CoA ligase OS=Bacillus subtilis (strain 168) GN=lcfB PE=2 SV=2)

HSP 1 Score: 253.4 bits (646), Expect = 5.1e-66
Identity = 163/512 (31.84%), Postives = 260/512 (50.78%), Query Frame = 1

Query: 10  LKKAASEFPRCRALSVSGKFDLTHERLQELIEHAAARLVDAGVKAGDVVALTFPNTVEYV 69
           L++ ASE P   A        +T++ L E I+  A  L +AG++ GD +AL   N+ +++
Sbjct: 7   LEETASEKPDSIACRFKDHM-MTYQELNEYIQRFADGLQEAGMEKGDHLALLLGNSPDFI 66

Query: 70  IMFLAVIRCRATAAPLNSAYTAEEFEFYLSDSESKLL----------------LTSKEGI 129
           I F   ++      P+N  YT  E  + L++ + K +                L   E +
Sbjct: 67  IAFFGALKAGIVVVPINPLYTPTEIGYMLTNGDVKAIVGVSQLLPLYESMHESLPKVELV 126

Query: 130 SSAQT--AATKLNIPQVKANLSSGDEFIELFPSPIETGSNVVEIVNDPSDAALFLHTSGT 189
              QT  A  +   P+V+  +++  + +     P        E V  P D A+ L+TSGT
Sbjct: 127 ILCQTGEAEPEAADPEVRMKMTTFAKILR----PTSAAKQNQEPV--PDDTAVILYTSGT 186

Query: 190 TSRPKGVPLTQQNLGASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLIAGAAVT 249
           T +PKG  LT QNL ++  ++     + E D+ V  LP+FHV  L   + + L++GA V 
Sbjct: 187 TGKPKGAMLTHQNLYSNANDVAGYLGMDERDNVVCALPMFHVFCLTVCMNAPLMSGATVL 246

Query: 250 LPAAGRFSASTFWSDMIAYNATWYTAVPTIHQIILERHLSKPEPSYPKLRFIRSCSASLA 309
           +    +FS ++ +  +    AT +  VPT++  + +    K +  +  +R   S  AS+ 
Sbjct: 247 IEP--QFSPASVFKLVKQQQATIFAGVPTMYNYLFQHENGKKD-DFSSIRLCISGGASMP 306

Query: 310 PSILERLEQSFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQ-EMAILDENG 369
            ++L   E+ FG  +LE Y ++EAS +   NP   D   K GS+G  I   E  ++D  G
Sbjct: 307 VALLTAFEEKFGVTILEGYGLSEASPVTCFNPF--DRGRKPGSIGTSILHVENKVVDPLG 366

Query: 370 AIQSEGVKGEVCIRGPNVTKGYKNNPDANNSAFMFGWFHTGDIGFFDSDGYLHLVGRIKE 429
                   GE+ ++GPNV KGY   P     A   GW +TGD+   D DGY ++V R K+
Sbjct: 367 RELPAHQVGELIVKGPNVMKGYYKMPMETEHALKDGWLYTGDLARRDEDGYFYIVDRKKD 426

Query: 430 LINRGGEKISPIEVDAVLLSHTDVSQAVAFGVPDNKYGEEINCAIIPREGSSINEADVLQ 489
           +I  GG  + P EV+ VL SH DV +AV  GVPD + GE +   ++P+  S + E D++Q
Sbjct: 427 MIIVGGYNVYPREVEEVLYSHPDVKEAVVIGVPDPQSGEAVKGYVVPKR-SGVTEEDIMQ 486

Query: 490 FCKKNLASFKVPKKVFITDYLPKTASGKIQRR 503
            C+K+LA +K P  +   D +PK A+GK+ RR
Sbjct: 487 HCEKHLAKYKRPAAITFLDDIPKNATGKMLRR 505

BLAST of Cucsa.303020 vs. TrEMBL
Match: A0A0A0KKA0_CUCSA (AMP dependent CoA ligase OS=Cucumis sativus GN=Csa_5G154230 PE=4 SV=1)

HSP 1 Score: 1011.1 bits (2613), Expect = 4.7e-292
Identity = 513/513 (100.00%), Postives = 513/513 (100.00%), Query Frame = 1

Query: 1   MENCTFTGLLKKAASEFPRCRALSVSGKFDLTHERLQELIEHAAARLVDAGVKAGDVVAL 60
           MENCTFTGLLKKAASEFPRCRALSVSGKFDLTHERLQELIEHAAARLVDAGVKAGDVVAL
Sbjct: 1   MENCTFTGLLKKAASEFPRCRALSVSGKFDLTHERLQELIEHAAARLVDAGVKAGDVVAL 60

Query: 61  TFPNTVEYVIMFLAVIRCRATAAPLNSAYTAEEFEFYLSDSESKLLLTSKEGISSAQTAA 120
           TFPNTVEYVIMFLAVIRCRATAAPLNSAYTAEEFEFYLSDSESKLLLTSKEGISSAQTAA
Sbjct: 61  TFPNTVEYVIMFLAVIRCRATAAPLNSAYTAEEFEFYLSDSESKLLLTSKEGISSAQTAA 120

Query: 121 TKLNIPQVKANLSSGDEFIELFPSPIETGSNVVEIVNDPSDAALFLHTSGTTSRPKGVPL 180
           TKLNIPQVKANLSSGDEFIELFPSPIETGSNVVEIVNDPSDAALFLHTSGTTSRPKGVPL
Sbjct: 121 TKLNIPQVKANLSSGDEFIELFPSPIETGSNVVEIVNDPSDAALFLHTSGTTSRPKGVPL 180

Query: 181 TQQNLGASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLIAGAAVTLPAAGRFSA 240
           TQQNLGASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLIAGAAVTLPAAGRFSA
Sbjct: 181 TQQNLGASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLIAGAAVTLPAAGRFSA 240

Query: 241 STFWSDMIAYNATWYTAVPTIHQIILERHLSKPEPSYPKLRFIRSCSASLAPSILERLEQ 300
           STFWSDMIAYNATWYTAVPTIHQIILERHLSKPEPSYPKLRFIRSCSASLAPSILERLEQ
Sbjct: 241 STFWSDMIAYNATWYTAVPTIHQIILERHLSKPEPSYPKLRFIRSCSASLAPSILERLEQ 300

Query: 301 SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENGAIQSEGVKGE 360
           SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENGAIQSEGVKGE
Sbjct: 301 SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENGAIQSEGVKGE 360

Query: 361 VCIRGPNVTKGYKNNPDANNSAFMFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKIS 420
           VCIRGPNVTKGYKNNPDANNSAFMFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKIS
Sbjct: 361 VCIRGPNVTKGYKNNPDANNSAFMFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKIS 420

Query: 421 PIEVDAVLLSHTDVSQAVAFGVPDNKYGEEINCAIIPREGSSINEADVLQFCKKNLASFK 480
           PIEVDAVLLSHTDVSQAVAFGVPDNKYGEEINCAIIPREGSSINEADVLQFCKKNLASFK
Sbjct: 421 PIEVDAVLLSHTDVSQAVAFGVPDNKYGEEINCAIIPREGSSINEADVLQFCKKNLASFK 480

Query: 481 VPKKVFITDYLPKTASGKIQRRFVAEHFLAAKA 514
           VPKKVFITDYLPKTASGKIQRRFVAEHFLAAKA
Sbjct: 481 VPKKVFITDYLPKTASGKIQRRFVAEHFLAAKA 513

BLAST of Cucsa.303020 vs. TrEMBL
Match: A0A067JRK0_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_20609 PE=4 SV=1)

HSP 1 Score: 818.9 bits (2114), Expect = 3.4e-234
Identity = 416/513 (81.09%), Postives = 454/513 (88.50%), Query Frame = 1

Query: 1   MENCTFTGLLKKAASEFPRCRALSVSGKFDLTHERLQELIEHAAARLVDAGVKAGDVVAL 60
           MEN T TGLLKK+A+EFP  RALSVSGK DLTH  LQ+LI+HAA+ LV AGV  GDVVAL
Sbjct: 1   MENLTLTGLLKKSAAEFPNSRALSVSGKLDLTHAELQDLIDHAASLLVAAGVAPGDVVAL 60

Query: 61  TFPNTVEYVIMFLAVIRCRATAAPLNSAYTAEEFEFYLSDSESKLLLTSKEGISSAQTAA 120
           TFPNTVEYVIMFLAVIRCRATAAPLN AYTAEEFEFYLSDSESKLL+TS+ GI SAQ AA
Sbjct: 61  TFPNTVEYVIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESKLLITSQVGIQSAQAAA 120

Query: 121 TKLNIPQVKANLSSGDEFIELFPSPIETGS---NVVEIVNDPSDAALFLHTSGTTSRPKG 180
           +KL IP V A L    + I L  S + T S   +V EIVND SD ALFLHTSGTTSRPKG
Sbjct: 121 SKLKIPHVAATLDVETKAISL--SSLNTESKFDSVAEIVNDSSDVALFLHTSGTTSRPKG 180

Query: 181 VPLTQQNLGASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLIAGAAVTLPAAGR 240
           VPLTQ NL +SV NIKSVY+LSESDSTVLVLPLFHVHGL+AGLLSSLI+GA+V LPAAGR
Sbjct: 181 VPLTQLNLASSVLNIKSVYRLSESDSTVLVLPLFHVHGLLAGLLSSLISGASVALPAAGR 240

Query: 241 FSASTFWSDMIAYNATWYTAVPTIHQIILERHLSKPEPSYPKLRFIRSCSASLAPSILER 300
           FSASTFW DMI YNATWYTAVPTIHQIIL+RH+SKPEP+YP+LRFIRSCSASLAPSIL+R
Sbjct: 241 FSASTFWQDMIIYNATWYTAVPTIHQIILDRHVSKPEPAYPELRFIRSCSASLAPSILDR 300

Query: 301 LEQSFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENGAIQSEGV 360
           LE++FGAPVLE+YAMTEASHLMASNPLPEDG HK+GSVGKP+GQEMAILDENG +Q  GV
Sbjct: 301 LEEAFGAPVLEAYAMTEASHLMASNPLPEDGGHKSGSVGKPVGQEMAILDENGVVQPAGV 360

Query: 361 KGEVCIRGPNVTKGYKNNPDANNSAFMFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGE 420
            GEVCIRG NVTKGYKNN +AN SAF FGWFHTGD+GFFDS+GYL LVGRIKELINRGGE
Sbjct: 361 SGEVCIRGANVTKGYKNNSEANKSAFQFGWFHTGDVGFFDSEGYLRLVGRIKELINRGGE 420

Query: 421 KISPIEVDAVLLSHTDVSQAVAFGVPDNKYGEEINCAIIPREGSSINEADVLQFCKKNLA 480
           KISPIEVDAVLL+H D++QAVAFGVPD KYGEEINCAIIPREGS I+EA+VL +CKKNLA
Sbjct: 421 KISPIEVDAVLLAHPDIAQAVAFGVPDEKYGEEINCAIIPREGSEIDEAEVLGYCKKNLA 480

Query: 481 SFKVPKKVFITDYLPKTASGKIQRRFVAEHFLA 511
            FKVPKKVFITD +PKTA+GKIQRR VAEHFLA
Sbjct: 481 GFKVPKKVFITDSVPKTATGKIQRRIVAEHFLA 511

BLAST of Cucsa.303020 vs. TrEMBL
Match: H9A1W0_CANSA (Acyl-activating enzyme 8 OS=Cannabis sativa PE=2 SV=1)

HSP 1 Score: 817.4 bits (2110), Expect = 9.9e-234
Identity = 410/511 (80.23%), Postives = 455/511 (89.04%), Query Frame = 1

Query: 3   NCTFTGLLKKAASEFPRCRALSVSGKFDLTHERLQELIEHAAARLVDAGVKAGDVVALTF 62
           N T TGLLKKAA EFP  RA+S+SGK+ LTH RLQE+++HAA+ L+ +G+  GDVVALTF
Sbjct: 4   NLTLTGLLKKAAVEFPDRRAISLSGKYHLTHARLQEIVDHAASLLLASGIGHGDVVALTF 63

Query: 63  PNTVEYVIMFLAVIRCRATAAPLNSAYTAEEFEFYLSDSESKLLLTSKEGISSAQTAATK 122
           PNTVEY++MFLAVIRCRA AAPLN+AYTAEEFEFYLSDSESKLL+T  EGI +AQ AA+K
Sbjct: 64  PNTVEYIVMFLAVIRCRAVAAPLNAAYTAEEFEFYLSDSESKLLITGPEGIEAAQAAASK 123

Query: 123 LNIPQVKANL-SSGDEFIELFPSPIETGS--NVVEIVNDPSDAALFLHTSGTTSRPKGVP 182
           LNI +V A L  SGD  I L  S     S  +V EI NDPSD ALFLHTSGTTSRPKGVP
Sbjct: 124 LNIVRVTATLPGSGDGLIALSSSANNESSLDSVAEITNDPSDVALFLHTSGTTSRPKGVP 183

Query: 183 LTQQNLGASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLIAGAAVTLPAAGRFS 242
           LTQ NL +SVQNIKSVYKL+ESDSTV+VLPLFHVHG++AGLLSSLIAGA+VTLPAAGRFS
Sbjct: 184 LTQLNLASSVQNIKSVYKLTESDSTVVVLPLFHVHGMIAGLLSSLIAGASVTLPAAGRFS 243

Query: 243 ASTFWSDMIAYNATWYTAVPTIHQIILERHLSKPEPSYPKLRFIRSCSASLAPSILERLE 302
           ASTFWSDMIA NATWYTAVPTIHQIILERHL+KPEP+YPKLRFIRSCSASLAPSI+ RLE
Sbjct: 244 ASTFWSDMIACNATWYTAVPTIHQIILERHLNKPEPTYPKLRFIRSCSASLAPSIMARLE 303

Query: 303 QSFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENGAIQSEGVKG 362
           +SFGAPVLE+YAMTEA+HLMASNPLPEDG HK GSVGKP+GQEMAILD+NG+ Q  GV G
Sbjct: 304 ESFGAPVLEAYAMTEAAHLMASNPLPEDGGHKPGSVGKPVGQEMAILDQNGSAQLAGVSG 363

Query: 363 EVCIRGPNVTKGYKNNPDANNSAFMFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKI 422
           EVCIRGPNVTKGYKNNP+AN +AF FGWFHTGD+G+FD DGYLHLVGRIKELINRGGEKI
Sbjct: 364 EVCIRGPNVTKGYKNNPEANKAAFQFGWFHTGDVGYFDEDGYLHLVGRIKELINRGGEKI 423

Query: 423 SPIEVDAVLLSHTDVSQAVAFGVPDNKYGEEINCAIIPREGSSINEADVLQFCKKNLASF 482
           SPIEVDAVLLSH D+S AVAFGVPD+KYGEEINCA+IPREGS ++E  VL+FCKKNLA+F
Sbjct: 424 SPIEVDAVLLSHPDISHAVAFGVPDDKYGEEINCAVIPREGSEVDEDVVLRFCKKNLATF 483

Query: 483 KVPKKVFITDYLPKTASGKIQRRFVAEHFLA 511
           KVPKKVFITD LPKTA+GKIQRR VAEHFLA
Sbjct: 484 KVPKKVFITDSLPKTATGKIQRRIVAEHFLA 514

BLAST of Cucsa.303020 vs. TrEMBL
Match: A0A059C1P9_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_E00577 PE=4 SV=1)

HSP 1 Score: 814.7 bits (2103), Expect = 6.4e-233
Identity = 406/507 (80.08%), Postives = 454/507 (89.55%), Query Frame = 1

Query: 5   TFTGLLKKAASEFPRCRALSVSGKFDLTHERLQELIEHAAARLVDAGVKAGDVVALTFPN 64
           T +GLL K AS+FP  RA+S  GKFDLTH RLQEL++HA+A L  +GV AGDVVALTFPN
Sbjct: 7   TLSGLLAKVASDFPDRRAISACGKFDLTHARLQELVDHASALLAVSGVGAGDVVALTFPN 66

Query: 65  TVEYVIMFLAVIRCRATAAPLNSAYTAEEFEFYLSDSESKLLLTSKEGISSAQTAATKLN 124
            VE+VIMFLAVIRCRATAAPLNSAYTAEEFEFYL+DSESKLLLT KEG   AQ+AA+KLN
Sbjct: 67  IVEFVIMFLAVIRCRATAAPLNSAYTAEEFEFYLADSESKLLLTPKEGNQPAQSAASKLN 126

Query: 125 IPQVKANLSSGDEFIELFPSPIETGSNVV-EIVNDPSDAALFLHTSGTTSRPKGVPLTQQ 184
           IP V A L S +  I L  + +E   +++ ++VN+PSD ALFLHTSGTTSRPKGVPLTQ 
Sbjct: 127 IPHVTAELHSPNSKITLSSTKVEPSVDLMSKVVNEPSDVALFLHTSGTTSRPKGVPLTQL 186

Query: 185 NLGASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLIAGAAVTLPAAGRFSASTF 244
           NL +SVQNIKSVYKLSE DSTV+VLPLFHVHGL+AGLLSSL+AGAAV LPAAGRFSASTF
Sbjct: 187 NLASSVQNIKSVYKLSEMDSTVIVLPLFHVHGLLAGLLSSLVAGAAVALPAAGRFSASTF 246

Query: 245 WSDMIAYNATWYTAVPTIHQIILERHLSKPEPSYPKLRFIRSCSASLAPSILERLEQSFG 304
           W+DMI+YNATWYTAVPTIHQIIL+RHLSKPE +YPKLRFIRSCSASLAPSI+ RLE++F 
Sbjct: 247 WADMISYNATWYTAVPTIHQIILDRHLSKPEQTYPKLRFIRSCSASLAPSIMARLEEAFD 306

Query: 305 APVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENGAIQSEGVKGEVCI 364
           APVLE+YAMTEA+HLMASNPLPEDG HKAGSVG+P+GQEMAILDE+GAIQ+EGV GEVCI
Sbjct: 307 APVLEAYAMTEAAHLMASNPLPEDGPHKAGSVGRPVGQEMAILDEHGAIQAEGVSGEVCI 366

Query: 365 RGPNVTKGYKNNPDANNSAFMFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKISPIE 424
           RGPNVTKGYKNNP+AN  AF FGWFHTGD+GF DSDGYLHLVGRIKELINRGGEKISPIE
Sbjct: 367 RGPNVTKGYKNNPEANKQAFQFGWFHTGDLGFMDSDGYLHLVGRIKELINRGGEKISPIE 426

Query: 425 VDAVLLSHTDVSQAVAFGVPDNKYGEEINCAIIPREGSSINEADVLQFCKKNLASFKVPK 484
           VDAVLL+H DV+QAVAFGVPD+KYGEEINCA+IPREG  + E DV+Q+C+KNLASFKVPK
Sbjct: 427 VDAVLLAHPDVAQAVAFGVPDDKYGEEINCAVIPREGVVVTEQDVIQYCRKNLASFKVPK 486

Query: 485 KVFITDYLPKTASGKIQRRFVAEHFLA 511
           KVFITD +PKTASGKIQRR VAEHFLA
Sbjct: 487 KVFITDSVPKTASGKIQRRIVAEHFLA 513

BLAST of Cucsa.303020 vs. TrEMBL
Match: I6XCT7_LINUS (Acyl CoA ligase OS=Linum usitatissimum PE=4 SV=1)

HSP 1 Score: 814.3 bits (2102), Expect = 8.4e-233
Identity = 404/507 (79.68%), Postives = 454/507 (89.55%), Query Frame = 1

Query: 5   TFTGLLKKAASEFPRCRALSVSGKFDLTHERLQELIEHAAARLVDAGVKAGDVVALTFPN 64
           T TGLLK+ A EFP  RALSVSGKFDLTH RL EL+E AA+RLV AG+K GDVVALTFPN
Sbjct: 6   TLTGLLKRVAGEFPDRRALSVSGKFDLTHARLDELVERAASRLVSAGIKPGDVVALTFPN 65

Query: 65  TVEYVIMFLAVIRCRATAAPLNSAYTAEEFEFYLSDSESKLLLTSKEGISSAQTAATKLN 124
           TVE+V+MFLAVIR RATAAPLN+AYTAEEFEFYLSDSESKLL+TS+EG ++A+ AA+KL 
Sbjct: 66  TVEFVVMFLAVIRARATAAPLNAAYTAEEFEFYLSDSESKLLVTSREGNAAAEAAASKLQ 125

Query: 125 IPQVKANLSSGDEFIELFPSPIETGSN-VVEIVNDPSDAALFLHTSGTTSRPKGVPLTQQ 184
           + QV A L+  D  + L  SP ETG N + ++VNDPSD ALFLHTSGTTSRPKGVPLTQ 
Sbjct: 126 VTQVTAGLTGEDSELILSLSPSETGDNSITQLVNDPSDVALFLHTSGTTSRPKGVPLTQL 185

Query: 185 NLGASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLIAGAAVTLPAAGRFSASTF 244
           NL +SVQNIKSVYKL+ESDSTV+VLPLFHVHGL+AGLLSS +AGAAVTLPA+GRFSAS+F
Sbjct: 186 NLASSVQNIKSVYKLTESDSTVIVLPLFHVHGLLAGLLSSFVAGAAVTLPASGRFSASSF 245

Query: 245 WSDMIAYNATWYTAVPTIHQIILERHLSKPEPSYPKLRFIRSCSASLAPSILERLEQSFG 304
           W DM+ YNATWYTAVPTIHQIIL+RH S PEP YPKLRFIRSCSASLAPSIL RLE++FG
Sbjct: 246 WQDMLKYNATWYTAVPTIHQIILDRHGSSPEPVYPKLRFIRSCSASLAPSILARLEEAFG 305

Query: 305 APVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENGAIQSEGVKGEVCI 364
           APVLE+YAMTEA+HLM+SNPLPEDG HK GSVGKP+GQEMAILDE+GAIQ    KGEVCI
Sbjct: 306 APVLEAYAMTEATHLMSSNPLPEDGPHKPGSVGKPVGQEMAILDESGAIQGPDSKGEVCI 365

Query: 365 RGPNVTKGYKNNPDANNSAFMFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKISPIE 424
           RGPNVTKGYKNNPDAN + F+FGWFHTGDIG+FDSDGYL+LVGRIKELINRGGEKISPIE
Sbjct: 366 RGPNVTKGYKNNPDANKAGFLFGWFHTGDIGYFDSDGYLNLVGRIKELINRGGEKISPIE 425

Query: 425 VDAVLLSHTDVSQAVAFGVPDNKYGEEINCAIIPREGSSINEADVLQFCKKNLASFKVPK 484
           VDAVLLSH D++QAV FGVPD+KYGEEINCAIIPREGS I+EA+V  FCKKNLA+FKVPK
Sbjct: 426 VDAVLLSHPDIAQAVCFGVPDDKYGEEINCAIIPREGSKIDEAEVQSFCKKNLAAFKVPK 485

Query: 485 KVFITDYLPKTASGKIQRRFVAEHFLA 511
           KVFIT+ +PKTASGKIQRR VA+HF++
Sbjct: 486 KVFITNDVPKTASGKIQRRIVAQHFIS 512

BLAST of Cucsa.303020 vs. TAIR10
Match: AT3G48990.1 (AT3G48990.1 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 773.5 bits (1996), Expect = 8.3e-224
Identity = 390/511 (76.32%), Postives = 438/511 (85.71%), Query Frame = 1

Query: 1   MENCTFTGLLKKAASEFPRCRALSVSGKFDLTHERLQELIEHAAARLV-DAGVKAGDVVA 60
           M++ T +GLL+  A +FP  RALSVSGKF+LTH RL +LIE AA+RLV DAG+K GDVVA
Sbjct: 1   MDSDTLSGLLENVAKKFPDRRALSVSGKFNLTHARLHDLIERAASRLVSDAGIKPGDVVA 60

Query: 61  LTFPNTVEYVIMFLAVIRCRATAAPLNSAYTAEEFEFYLSDSESKLLLTSKEGISSAQTA 120
           LTFPNTVE+VIMFLAVIR RATAAPLN+AYTAEEFEFYLSDS+SKLLLTSKEG + AQ A
Sbjct: 61  LTFPNTVEFVIMFLAVIRARATAAPLNAAYTAEEFEFYLSDSDSKLLLTSKEGNAPAQEA 120

Query: 121 ATKLNIPQVKANL-SSGDEFIELFPSPIETGSNVVEIVNDPSDAALFLHTSGTTSRPKGV 180
           A+KL I  V A L  +G + +           +  E+VN P D ALFLHTSGTTSRPKGV
Sbjct: 121 ASKLKISHVTATLLDAGSDLVLSVADSDSVVDSATELVNHPDDGALFLHTSGTTSRPKGV 180

Query: 181 PLTQQNLGASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLIAGAAVTLPAAGRF 240
           PLTQ NL +SV+NIK+VYKL+ESDSTV+VLPLFHVHGL+AGLLSSL AGAAVTLPAAGRF
Sbjct: 181 PLTQLNLASSVKNIKAVYKLTESDSTVIVLPLFHVHGLLAGLLSSLGAGAAVTLPAAGRF 240

Query: 241 SASTFWSDMIAYNATWYTAVPTIHQIILERHLSKPEPSYPKLRFIRSCSASLAPSILERL 300
           SA+TFW DM  YNATWYTAVPTIHQIIL+RH S PE  YPKLRFIRSCSASLAP IL RL
Sbjct: 241 SATTFWPDMKKYNATWYTAVPTIHQIILDRHASHPETEYPKLRFIRSCSASLAPVILSRL 300

Query: 301 EQSFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENGAIQSEGVK 360
           E++FGAPVLE+YAMTEA+HLM+SNPLPE+G HK GSVGKP+GQEMAIL+E G IQ    K
Sbjct: 301 EEAFGAPVLEAYAMTEATHLMSSNPLPEEGPHKPGSVGKPVGQEMAILNEKGEIQEPNNK 360

Query: 361 GEVCIRGPNVTKGYKNNPDANNSAFMFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEK 420
           GEVCIRGPNVTKGYKNNP+AN + F FGWFHTGDIG+FD+DGYLHLVGRIKELINRGGEK
Sbjct: 361 GEVCIRGPNVTKGYKNNPEANKAGFEFGWFHTGDIGYFDTDGYLHLVGRIKELINRGGEK 420

Query: 421 ISPIEVDAVLLSHTDVSQAVAFGVPDNKYGEEINCAIIPREGSSINEADVLQFCKKNLAS 480
           ISPIEVDAVLL+H DVSQ VAFGVPD KYGEEINCA+IPREG+++ E D+  FCKKNLA+
Sbjct: 421 ISPIEVDAVLLTHPDVSQGVAFGVPDEKYGEEINCAVIPREGTTVTEEDIKAFCKKNLAA 480

Query: 481 FKVPKKVFITDYLPKTASGKIQRRFVAEHFL 510
           FKVPK+VFITD LPKTASGKIQRR VA+HFL
Sbjct: 481 FKVPKRVFITDNLPKTASGKIQRRIVAQHFL 511

BLAST of Cucsa.303020 vs. TAIR10
Match: AT4G05160.1 (AT4G05160.1 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 203.8 bits (517), Expect = 2.6e-52
Identity = 155/521 (29.75%), Postives = 249/521 (47.79%), Query Frame = 1

Query: 3   NCTFTGLLKKAASEFPRCRALSVSGKFD-LTHERLQELIEHAAARLVDAGVKAGDVVALT 62
           N +    L + +S +P   A++ S   D LT  +L+  +   A      G++  DVV + 
Sbjct: 26  NTSLVSFLFRNSSSYPSKLAIADSDTGDSLTFSQLKSAVARLAHGFHRLGIRKNDVVLIF 85

Query: 63  FPNTVEYVIMFLAVIRCRATAAPLNSAYTAEEFEFYLSDSESKLLLTSKEGISSAQTAAT 122
            PN+ ++ + FLAV          N  YT  E    + DS  K++++  +     +    
Sbjct: 86  APNSYQFPLCFLAVTAIGGVFTTANPLYTVNEVSKQIKDSNPKIIISVNQLFDKIKG--- 145

Query: 123 KLNIPQVKANLSSGDEFIELFP---SPIETGSNVVEIVNDP-----------SDAALFLH 182
             ++P V   L    + +E+ P   S I +  NV+E+ ++P           SD A  L+
Sbjct: 146 -FDLPVV---LLGSKDTVEIPPGSNSKILSFDNVMEL-SEPVSEYPFVEIKQSDTAALLY 205

Query: 183 TSGTTSRPKGVPLTQQN-LGASVQNIKSVYKLSESDSTVLV-LPLFHVHGLMAGLLSSLI 242
           +SGTT   KGV LT  N + AS+        + E     L  LP+FHV GL     S L 
Sbjct: 206 SSGTTGTSKGVELTHGNFIAASLMVTMDQDLMGEYHGVFLCFLPMFHVFGLAVITYSQLQ 265

Query: 243 AGAAVTLPAAGRFSASTFWSDMIAYNATWYTAVPTIHQIILERHLSKPEPSYPKLRFIRS 302
            G A  L +  RF       ++  +  T    VP +   + ++ + K +     L++I S
Sbjct: 266 RGNA--LVSMARFELELVLKNIEKFRVTHLWVVPPVFLALSKQSIVK-KFDLSSLKYIGS 325

Query: 303 CSASLAPSILERLEQSF-GAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGK--PIGQE 362
            +A L   ++E   ++     +++ Y MTE   +++    P  G   +GS G   P  + 
Sbjct: 326 GAAPLGKDLMEECGRNIPNVLLMQGYGMTETCGIVSVED-PRLGKRNSGSAGMLAPGVEA 385

Query: 363 MAILDENGAIQSEGVKGEVCIRGPNVTKGYKNNPDANNSAF-MFGWFHTGDIGFFDSDGY 422
             +  E G  Q    +GE+ +RGPN+ KGY NNP A         W HTGD+G+F+ DG 
Sbjct: 386 QIVSVETGKSQPPNQQGEIWVRGPNMMKGYLNNPQATKETIDKKSWVHTGDLGYFNEDGN 445

Query: 423 LHLVGRIKELINRGGEKISPIEVDAVLLSHTDVSQAVAFGVPDNKYGEEINCAIIPREGS 482
           L++V RIKELI   G +++P E++ +L+SH D+  AV    PD + GE     ++    S
Sbjct: 446 LYVVDRIKELIKYKGFQVAPAELEGLLVSHPDILDAVVIPFPDEEAGEVPIAFVVRSPNS 505

Query: 483 SINEADVLQFCKKNLASFKVPKKVFITDYLPKTASGKIQRR 503
           SI E D+ +F  K +A +K  ++V     +PK+A+GKI RR
Sbjct: 506 SITEQDIQKFIAKQVAPYKRLRRVSFISLVPKSAAGKILRR 534

BLAST of Cucsa.303020 vs. TAIR10
Match: AT1G51680.1 (AT1G51680.1 4-coumarate:CoA ligase 1)

HSP 1 Score: 195.3 bits (495), Expect = 9.3e-50
Identity = 150/494 (30.36%), Postives = 227/494 (45.95%), Query Frame = 1

Query: 32  THERLQELIEHAAARLVDAGVKAGDVVALTFPNTVEYVIMFLAVIRCRATAAPLNSAYTA 91
           T+  +  +    AA     GV   DVV L  PN  E+V+ FLA     ATA   N  +T 
Sbjct: 67  TYSDVHVISRQIAANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTP 126

Query: 92  EEFEFYLSDSESKLLLTSKEGISSAQTAATKLNIPQVKANLSSGD-------EFIELFPS 151
            E       S +KL++T    +   +       +  V  + +           F EL  S
Sbjct: 127 AEIAKQAKASNTKLIITEARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQS 186

Query: 152 PIETGSNVVEIVNDPSDAALFLHTSGTTSRPKGVPLTQQNLGASV-QNI--KSVYKLSES 211
             E    +  +   P D     ++SGTT  PKGV LT + L  SV Q +  ++      S
Sbjct: 187 TTEASEVIDSVEISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHS 246

Query: 212 DSTVL-VLPLFHVHGLMAGLLSSLIAGAAVTLPAAGRFSASTFWSDMIAYNATWYTAVPT 271
           D  +L VLP+FH++ L + +L  L  GAA+ +    +F  +     +        T  P 
Sbjct: 247 DDVILCVLPMFHIYALNSIMLCGLRVGAAILIMP--KFEINLL---LELIQRCKVTVAPM 306

Query: 272 IHQIILERHLSKPEPSYP--KLRFIRSCSASLAPSILERLEQSF-GAPVLESYAMTEASH 331
           +  I+L    S     Y    +R ++S +A L   + + +   F  A + + Y MTEA  
Sbjct: 307 VPPIVLAIAKSSETEKYDLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGP 366

Query: 332 LMASN------PLPEDGVHKAGSVGKPI-GQEMAILD-ENGAIQSEGVKGEVCIRGPNVT 391
           ++A +      P P     K+G+ G  +   EM I+D + G   S    GE+CIRG  + 
Sbjct: 367 VLAMSLGFAKEPFPV----KSGACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIM 426

Query: 392 KGYKNNPDANNSAF-MFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKISPIEVDAVL 451
           KGY NNP A        GW HTGDIG  D D  L +V R+KELI   G +++P E++A+L
Sbjct: 427 KGYLNNPAATAETIDKDGWLHTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALL 486

Query: 452 LSHTDVSQAVAFGVPDNKYGEEINCAIIPREGSSINEADVLQFCKKNLASFKVPKKVFIT 503
           + H D++      + +   GE     ++  + S ++E DV QF  K +  +K   KVF T
Sbjct: 487 IGHPDITDVAVVAMKEEAAGEVPVAFVVKSKDSELSEDDVKQFVSKQVVFYKRINKVFFT 546

BLAST of Cucsa.303020 vs. TAIR10
Match: AT1G20510.1 (AT1G20510.1 OPC-8:0 CoA ligase1)

HSP 1 Score: 194.9 bits (494), Expect = 1.2e-49
Identity = 141/483 (29.19%), Postives = 238/483 (49.28%), Query Frame = 1

Query: 30  DLTHERLQELIEHAAARLVDAGVKAGDVVALTFPNTVEYVIMFLAVIRCRATAAPLNSAY 89
           +LT   L   +E  A  L + G++ G VV L  PN++ + ++ L+V+   A     N   
Sbjct: 57  NLTFTELWRAVESVADCLSEIGIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPLN 116

Query: 90  TAEEFEFYLSDSESKLLLTSKEGISSAQTAATKLNIPQVKANL--SSGD--EFIELFPSP 149
           T+ E    + DS   L  T+ + +     AA KL I  +      S GD    +E+    
Sbjct: 117 TSNEIAKQIKDSNPVLAFTTSQLLPKISAAAKKLPIVLMDEERVDSVGDVRRLVEMMKKE 176

Query: 150 IETGSNVVEIVNDPSDAALFLHTSGTTSRPKGVPLTQQNLGASVQNIKSVYKLSESDST- 209
             +G+ V E V D  D A  L++SGTT   KGV  + +NL A VQ I + +   + +   
Sbjct: 177 -PSGNRVKERV-DQDDTATLLYSSGTTGMSKGVISSHRNLIAMVQTIVNRFGSDDGEQRF 236

Query: 210 VLVLPLFHVHGLMAGLLSSLIAGAAVTLPAAGRFSASTFWSDMIAYNATWYTAVPTIHQI 269
           +  +P+FH++GL A     L  G+  T+    +F      S +  Y AT    VP I   
Sbjct: 237 ICTVPMFHIYGLAAFATGLLAYGS--TIIVLSKFEMHEMMSAIGKYQATSLPLVPPILVA 296

Query: 270 ILE-RHLSKPEPSYPKLRFIRSCSASLAPSILERLEQSFGA-PVLESYAMTEASHLMASN 329
           ++      K +     +  +    A L+  + E   + +    +L+ Y +TE++ + AS 
Sbjct: 297 MVNGADQIKAKYDLSSMHTVLCGGAPLSKEVTEGFAEKYPTVKILQGYGLTESTGIGAST 356

Query: 330 PLPEDGVHKAGSVGK-PIGQEMAILDE-NGAIQSEGVKGEVCIRGPNVTKGYKNNPDANN 389
              E+   + G+ GK     E  I+D   G I      GE+ ++GP++ KGY +N +A +
Sbjct: 357 DTVEES-RRYGTAGKLSASMEGRIVDPVTGQILGPKQTGELWLKGPSIMKGYFSNEEATS 416

Query: 390 SAF-MFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKISPIEVDAVLLSHTDVSQAVA 449
           S     GW  TGD+ + D DG++ +V R+KELI   G +++P E++A+LL+H +++ A  
Sbjct: 417 STLDSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLTHPEITDAAV 476

Query: 450 FGVPDNKYGEEINCAIIPREGSSINEADVLQFCKKNLASFKVPKKVFITDYLPKTASGKI 503
              PD + G+     ++ + GSS++E  +++F  K +A +K  +KV     +PK  SGKI
Sbjct: 477 IPFPDKEVGQFPMAYVVRKTGSSLSEKTIMEFVAKQVAPYKRIRKVAFVSSIPKNPSGKI 534

BLAST of Cucsa.303020 vs. TAIR10
Match: AT4G19010.1 (AT4G19010.1 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 194.5 bits (493), Expect = 1.6e-49
Identity = 138/485 (28.45%), Postives = 247/485 (50.93%), Query Frame = 1

Query: 29  FDLTHERLQELIEHAAARLVDA-GVKAGDVVALTFPNTVEYVIMFLAVIRCRATAAPLNS 88
           F ++H  LQ +++  AA +    GV+ GDVV+L  PN+V + ++FL++I   A    +N 
Sbjct: 71  FSISHTELQIMVQSMAAGIYHVLGVRQGDVVSLVLPNSVYFPMIFLSLISLGAIVTTMNP 130

Query: 89  AYTAEEFEFYLSDSESKLLLTSKEGISSAQTAATK-LNIPQVKANLSSGDEFIELFPSPI 148
           + +  E +  +S+    L  TS E +    +     +++ +     S   E  + +    
Sbjct: 131 SSSLGEIKKQVSECSVGLAFTSTENVEKLSSLGVSVISVSESYDFDSIRIENPKFYSIMK 190

Query: 149 ETGSNVVEIVNDPSDAALFLHTSGTTSRPKGVPLTQQNLGASVQNI----KSVYKLSESD 208
           E+   V + +    D A  +++SGTT   KGV LT +NL AS++       S Y+   S 
Sbjct: 191 ESFGFVPKPLIKQDDVAAIMYSSGTTGASKGVLLTHRNLIASMELFVRFEASQYEYPGSS 250

Query: 209 STVLV-LPLFHVHGLMAGLLSSLIAGAAVTLPAAGRFSASTFWSDMIAYNATWYTAVPTI 268
           +  L  LPL H++GL   ++  L  G+  T+    RF AS   + +  +  T +  VP +
Sbjct: 251 NVYLAALPLCHIYGLSLFVMGLLSLGS--TIVVMKRFDASDVVNVIERFKITHFPVVPPM 310

Query: 269 HQIILERHLSKPEPSYPKLRFIRSCSASLAPSILERLEQSFG-APVLESYAMTEASHLMA 328
              + ++        +  L+ + S +A L+   +E   Q+     +++ Y MTE++  + 
Sbjct: 311 LMALTKKAKGVCGEVFKSLKQVSSGAAPLSRKFIEDFLQTLPHVDLIQGYGMTEST-AVG 370

Query: 329 SNPLPEDGVHKAGSVG--KPIGQEMAILDENGAIQSEGVKGEVCIRGPNVTKGYKNNPDA 388
           +     + + +  SVG   P  Q   +   +G+    G +GE+ I+GP V KGY NNP A
Sbjct: 371 TRGFNSEKLSRYSSVGLLAPNMQAKVVDWSSGSFLPPGNRGELWIQGPGVMKGYLNNPKA 430

Query: 389 NNSAFMF-GWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKISPIEVDAVLLSHTDVSQA 448
              + +   W  TGDI +FD DGYL +V RIKE+I   G +I+P +++AVL+SH  +  A
Sbjct: 431 TQMSIVEDSWLRTGDIAYFDEDGYLFIVDRIKEIIKYKGFQIAPADLEAVLVSHPLIIDA 490

Query: 449 VAFGVPDNKYGEEINCAIIPREGSSINEADVLQFCKKNLASFKVPKKVFITDYLPKTASG 503
                P+ + GE     ++ R+ ++++E DV+ +    +A ++  +KV + + +PK+ +G
Sbjct: 491 AVTAAPNEECGEIPVAFVVRRQETTLSEEDVISYVASQVAPYRKVRKVVMVNSIPKSPTG 550

BLAST of Cucsa.303020 vs. NCBI nr
Match: gi|449452338|ref|XP_004143916.1| (PREDICTED: oxalate--CoA ligase-like [Cucumis sativus])

HSP 1 Score: 1011.1 bits (2613), Expect = 6.7e-292
Identity = 513/513 (100.00%), Postives = 513/513 (100.00%), Query Frame = 1

Query: 1   MENCTFTGLLKKAASEFPRCRALSVSGKFDLTHERLQELIEHAAARLVDAGVKAGDVVAL 60
           MENCTFTGLLKKAASEFPRCRALSVSGKFDLTHERLQELIEHAAARLVDAGVKAGDVVAL
Sbjct: 1   MENCTFTGLLKKAASEFPRCRALSVSGKFDLTHERLQELIEHAAARLVDAGVKAGDVVAL 60

Query: 61  TFPNTVEYVIMFLAVIRCRATAAPLNSAYTAEEFEFYLSDSESKLLLTSKEGISSAQTAA 120
           TFPNTVEYVIMFLAVIRCRATAAPLNSAYTAEEFEFYLSDSESKLLLTSKEGISSAQTAA
Sbjct: 61  TFPNTVEYVIMFLAVIRCRATAAPLNSAYTAEEFEFYLSDSESKLLLTSKEGISSAQTAA 120

Query: 121 TKLNIPQVKANLSSGDEFIELFPSPIETGSNVVEIVNDPSDAALFLHTSGTTSRPKGVPL 180
           TKLNIPQVKANLSSGDEFIELFPSPIETGSNVVEIVNDPSDAALFLHTSGTTSRPKGVPL
Sbjct: 121 TKLNIPQVKANLSSGDEFIELFPSPIETGSNVVEIVNDPSDAALFLHTSGTTSRPKGVPL 180

Query: 181 TQQNLGASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLIAGAAVTLPAAGRFSA 240
           TQQNLGASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLIAGAAVTLPAAGRFSA
Sbjct: 181 TQQNLGASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLIAGAAVTLPAAGRFSA 240

Query: 241 STFWSDMIAYNATWYTAVPTIHQIILERHLSKPEPSYPKLRFIRSCSASLAPSILERLEQ 300
           STFWSDMIAYNATWYTAVPTIHQIILERHLSKPEPSYPKLRFIRSCSASLAPSILERLEQ
Sbjct: 241 STFWSDMIAYNATWYTAVPTIHQIILERHLSKPEPSYPKLRFIRSCSASLAPSILERLEQ 300

Query: 301 SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENGAIQSEGVKGE 360
           SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENGAIQSEGVKGE
Sbjct: 301 SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENGAIQSEGVKGE 360

Query: 361 VCIRGPNVTKGYKNNPDANNSAFMFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKIS 420
           VCIRGPNVTKGYKNNPDANNSAFMFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKIS
Sbjct: 361 VCIRGPNVTKGYKNNPDANNSAFMFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKIS 420

Query: 421 PIEVDAVLLSHTDVSQAVAFGVPDNKYGEEINCAIIPREGSSINEADVLQFCKKNLASFK 480
           PIEVDAVLLSHTDVSQAVAFGVPDNKYGEEINCAIIPREGSSINEADVLQFCKKNLASFK
Sbjct: 421 PIEVDAVLLSHTDVSQAVAFGVPDNKYGEEINCAIIPREGSSINEADVLQFCKKNLASFK 480

Query: 481 VPKKVFITDYLPKTASGKIQRRFVAEHFLAAKA 514
           VPKKVFITDYLPKTASGKIQRRFVAEHFLAAKA
Sbjct: 481 VPKKVFITDYLPKTASGKIQRRFVAEHFLAAKA 513

BLAST of Cucsa.303020 vs. NCBI nr
Match: gi|659073781|ref|XP_008437250.1| (PREDICTED: 4-coumarate--CoA ligase-like 10 [Cucumis melo])

HSP 1 Score: 968.0 bits (2501), Expect = 6.5e-279
Identity = 492/513 (95.91%), Postives = 499/513 (97.27%), Query Frame = 1

Query: 1   MENCTFTGLLKKAASEFPRCRALSVSGKFDLTHERLQELIEHAAARLVDAGVKAGDVVAL 60
           MENCT TGLLKKAASEFP  RALSVSGKFDLTHERLQELIEHAAARLVD+GV AGDVVAL
Sbjct: 1   MENCTLTGLLKKAASEFPHRRALSVSGKFDLTHERLQELIEHAAARLVDSGVNAGDVVAL 60

Query: 61  TFPNTVEYVIMFLAVIRCRATAAPLNSAYTAEEFEFYLSDSESKLLLTSKEGISSAQTAA 120
           TF NTVEYVIMFLAVIRCRATAAPLNSAYTAEEFEFYLSDSESKLLLTSKEGISSAQTAA
Sbjct: 61  TFLNTVEYVIMFLAVIRCRATAAPLNSAYTAEEFEFYLSDSESKLLLTSKEGISSAQTAA 120

Query: 121 TKLNIPQVKANLSSGDEFIELFPSPIETGSNVVEIVNDPSDAALFLHTSGTTSRPKGVPL 180
           TKLNIPQVK +LSSGDE+IELFPSPIE  SNVVEIVNDPSDAALFLHTSGTTSRPKGVPL
Sbjct: 121 TKLNIPQVKVSLSSGDEYIELFPSPIEMSSNVVEIVNDPSDAALFLHTSGTTSRPKGVPL 180

Query: 181 TQQNLGASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLIAGAAVTLPAAGRFSA 240
           TQQNL ASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLIAGAAVTLPAAGRFSA
Sbjct: 181 TQQNLAASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLIAGAAVTLPAAGRFSA 240

Query: 241 STFWSDMIAYNATWYTAVPTIHQIILERHLSKPEPSYPKLRFIRSCSASLAPSILERLEQ 300
           +TFWSDMIAYNATWYTAVPTIHQIILERH SKPEPSYPKLRFIRSCSASLAPSILERLEQ
Sbjct: 241 TTFWSDMIAYNATWYTAVPTIHQIILERHFSKPEPSYPKLRFIRSCSASLAPSILERLEQ 300

Query: 301 SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENGAIQSEGVKGE 360
           SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENGAIQSEGVKGE
Sbjct: 301 SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENGAIQSEGVKGE 360

Query: 361 VCIRGPNVTKGYKNNPDANNSAFMFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKIS 420
           VCIRGPNVTKGYKNNPDAN SAFMFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKIS
Sbjct: 361 VCIRGPNVTKGYKNNPDANKSAFMFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKIS 420

Query: 421 PIEVDAVLLSHTDVSQAVAFGVPDNKYGEEINCAIIPREGSSINEADVLQFCKKNLASFK 480
           PIEVDAVLLSH D+SQAVAFGVPDNKYGEEINCAIIPREGSSINEADVL+FCKKNLASFK
Sbjct: 421 PIEVDAVLLSHPDISQAVAFGVPDNKYGEEINCAIIPREGSSINEADVLEFCKKNLASFK 480

Query: 481 VPKKVFITDYLPKTASGKIQRRFVAEHFLAAKA 514
           VPKKVFITD LPKTA+GKIQRR VAEHFLAAKA
Sbjct: 481 VPKKVFITDSLPKTATGKIQRRIVAEHFLAAKA 513

BLAST of Cucsa.303020 vs. NCBI nr
Match: gi|802740718|ref|XP_012086931.1| (PREDICTED: oxalate--CoA ligase-like [Jatropha curcas])

HSP 1 Score: 818.9 bits (2114), Expect = 4.9e-234
Identity = 416/513 (81.09%), Postives = 454/513 (88.50%), Query Frame = 1

Query: 1   MENCTFTGLLKKAASEFPRCRALSVSGKFDLTHERLQELIEHAAARLVDAGVKAGDVVAL 60
           MEN T TGLLKK+A+EFP  RALSVSGK DLTH  LQ+LI+HAA+ LV AGV  GDVVAL
Sbjct: 1   MENLTLTGLLKKSAAEFPNSRALSVSGKLDLTHAELQDLIDHAASLLVAAGVAPGDVVAL 60

Query: 61  TFPNTVEYVIMFLAVIRCRATAAPLNSAYTAEEFEFYLSDSESKLLLTSKEGISSAQTAA 120
           TFPNTVEYVIMFLAVIRCRATAAPLN AYTAEEFEFYLSDSESKLL+TS+ GI SAQ AA
Sbjct: 61  TFPNTVEYVIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESKLLITSQVGIQSAQAAA 120

Query: 121 TKLNIPQVKANLSSGDEFIELFPSPIETGS---NVVEIVNDPSDAALFLHTSGTTSRPKG 180
           +KL IP V A L    + I L  S + T S   +V EIVND SD ALFLHTSGTTSRPKG
Sbjct: 121 SKLKIPHVAATLDVETKAISL--SSLNTESKFDSVAEIVNDSSDVALFLHTSGTTSRPKG 180

Query: 181 VPLTQQNLGASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLIAGAAVTLPAAGR 240
           VPLTQ NL +SV NIKSVY+LSESDSTVLVLPLFHVHGL+AGLLSSLI+GA+V LPAAGR
Sbjct: 181 VPLTQLNLASSVLNIKSVYRLSESDSTVLVLPLFHVHGLLAGLLSSLISGASVALPAAGR 240

Query: 241 FSASTFWSDMIAYNATWYTAVPTIHQIILERHLSKPEPSYPKLRFIRSCSASLAPSILER 300
           FSASTFW DMI YNATWYTAVPTIHQIIL+RH+SKPEP+YP+LRFIRSCSASLAPSIL+R
Sbjct: 241 FSASTFWQDMIIYNATWYTAVPTIHQIILDRHVSKPEPAYPELRFIRSCSASLAPSILDR 300

Query: 301 LEQSFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENGAIQSEGV 360
           LE++FGAPVLE+YAMTEASHLMASNPLPEDG HK+GSVGKP+GQEMAILDENG +Q  GV
Sbjct: 301 LEEAFGAPVLEAYAMTEASHLMASNPLPEDGGHKSGSVGKPVGQEMAILDENGVVQPAGV 360

Query: 361 KGEVCIRGPNVTKGYKNNPDANNSAFMFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGE 420
            GEVCIRG NVTKGYKNN +AN SAF FGWFHTGD+GFFDS+GYL LVGRIKELINRGGE
Sbjct: 361 SGEVCIRGANVTKGYKNNSEANKSAFQFGWFHTGDVGFFDSEGYLRLVGRIKELINRGGE 420

Query: 421 KISPIEVDAVLLSHTDVSQAVAFGVPDNKYGEEINCAIIPREGSSINEADVLQFCKKNLA 480
           KISPIEVDAVLL+H D++QAVAFGVPD KYGEEINCAIIPREGS I+EA+VL +CKKNLA
Sbjct: 421 KISPIEVDAVLLAHPDIAQAVAFGVPDEKYGEEINCAIIPREGSEIDEAEVLGYCKKNLA 480

Query: 481 SFKVPKKVFITDYLPKTASGKIQRRFVAEHFLA 511
            FKVPKKVFITD +PKTA+GKIQRR VAEHFLA
Sbjct: 481 GFKVPKKVFITDSVPKTATGKIQRRIVAEHFLA 511

BLAST of Cucsa.303020 vs. NCBI nr
Match: gi|380042376|gb|AFD33352.1| (acyl-activating enzyme 8 [Cannabis sativa])

HSP 1 Score: 817.4 bits (2110), Expect = 1.4e-233
Identity = 410/511 (80.23%), Postives = 455/511 (89.04%), Query Frame = 1

Query: 3   NCTFTGLLKKAASEFPRCRALSVSGKFDLTHERLQELIEHAAARLVDAGVKAGDVVALTF 62
           N T TGLLKKAA EFP  RA+S+SGK+ LTH RLQE+++HAA+ L+ +G+  GDVVALTF
Sbjct: 4   NLTLTGLLKKAAVEFPDRRAISLSGKYHLTHARLQEIVDHAASLLLASGIGHGDVVALTF 63

Query: 63  PNTVEYVIMFLAVIRCRATAAPLNSAYTAEEFEFYLSDSESKLLLTSKEGISSAQTAATK 122
           PNTVEY++MFLAVIRCRA AAPLN+AYTAEEFEFYLSDSESKLL+T  EGI +AQ AA+K
Sbjct: 64  PNTVEYIVMFLAVIRCRAVAAPLNAAYTAEEFEFYLSDSESKLLITGPEGIEAAQAAASK 123

Query: 123 LNIPQVKANL-SSGDEFIELFPSPIETGS--NVVEIVNDPSDAALFLHTSGTTSRPKGVP 182
           LNI +V A L  SGD  I L  S     S  +V EI NDPSD ALFLHTSGTTSRPKGVP
Sbjct: 124 LNIVRVTATLPGSGDGLIALSSSANNESSLDSVAEITNDPSDVALFLHTSGTTSRPKGVP 183

Query: 183 LTQQNLGASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLIAGAAVTLPAAGRFS 242
           LTQ NL +SVQNIKSVYKL+ESDSTV+VLPLFHVHG++AGLLSSLIAGA+VTLPAAGRFS
Sbjct: 184 LTQLNLASSVQNIKSVYKLTESDSTVVVLPLFHVHGMIAGLLSSLIAGASVTLPAAGRFS 243

Query: 243 ASTFWSDMIAYNATWYTAVPTIHQIILERHLSKPEPSYPKLRFIRSCSASLAPSILERLE 302
           ASTFWSDMIA NATWYTAVPTIHQIILERHL+KPEP+YPKLRFIRSCSASLAPSI+ RLE
Sbjct: 244 ASTFWSDMIACNATWYTAVPTIHQIILERHLNKPEPTYPKLRFIRSCSASLAPSIMARLE 303

Query: 303 QSFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENGAIQSEGVKG 362
           +SFGAPVLE+YAMTEA+HLMASNPLPEDG HK GSVGKP+GQEMAILD+NG+ Q  GV G
Sbjct: 304 ESFGAPVLEAYAMTEAAHLMASNPLPEDGGHKPGSVGKPVGQEMAILDQNGSAQLAGVSG 363

Query: 363 EVCIRGPNVTKGYKNNPDANNSAFMFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKI 422
           EVCIRGPNVTKGYKNNP+AN +AF FGWFHTGD+G+FD DGYLHLVGRIKELINRGGEKI
Sbjct: 364 EVCIRGPNVTKGYKNNPEANKAAFQFGWFHTGDVGYFDEDGYLHLVGRIKELINRGGEKI 423

Query: 423 SPIEVDAVLLSHTDVSQAVAFGVPDNKYGEEINCAIIPREGSSINEADVLQFCKKNLASF 482
           SPIEVDAVLLSH D+S AVAFGVPD+KYGEEINCA+IPREGS ++E  VL+FCKKNLA+F
Sbjct: 424 SPIEVDAVLLSHPDISHAVAFGVPDDKYGEEINCAVIPREGSEVDEDVVLRFCKKNLATF 483

Query: 483 KVPKKVFITDYLPKTASGKIQRRFVAEHFLA 511
           KVPKKVFITD LPKTA+GKIQRR VAEHFLA
Sbjct: 484 KVPKKVFITDSLPKTATGKIQRRIVAEHFLA 514

BLAST of Cucsa.303020 vs. NCBI nr
Match: gi|702336860|ref|XP_010055622.1| (PREDICTED: 4-coumarate--CoA ligase-like 10 [Eucalyptus grandis])

HSP 1 Score: 814.7 bits (2103), Expect = 9.2e-233
Identity = 406/507 (80.08%), Postives = 454/507 (89.55%), Query Frame = 1

Query: 5   TFTGLLKKAASEFPRCRALSVSGKFDLTHERLQELIEHAAARLVDAGVKAGDVVALTFPN 64
           T +GLL K AS+FP  RA+S  GKFDLTH RLQEL++HA+A L  +GV AGDVVALTFPN
Sbjct: 7   TLSGLLAKVASDFPDRRAISACGKFDLTHARLQELVDHASALLAVSGVGAGDVVALTFPN 66

Query: 65  TVEYVIMFLAVIRCRATAAPLNSAYTAEEFEFYLSDSESKLLLTSKEGISSAQTAATKLN 124
            VE+VIMFLAVIRCRATAAPLNSAYTAEEFEFYL+DSESKLLLT KEG   AQ+AA+KLN
Sbjct: 67  IVEFVIMFLAVIRCRATAAPLNSAYTAEEFEFYLADSESKLLLTPKEGNQPAQSAASKLN 126

Query: 125 IPQVKANLSSGDEFIELFPSPIETGSNVV-EIVNDPSDAALFLHTSGTTSRPKGVPLTQQ 184
           IP V A L S +  I L  + +E   +++ ++VN+PSD ALFLHTSGTTSRPKGVPLTQ 
Sbjct: 127 IPHVTAELHSPNSKITLSSTKVEPSVDLMSKVVNEPSDVALFLHTSGTTSRPKGVPLTQL 186

Query: 185 NLGASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLIAGAAVTLPAAGRFSASTF 244
           NL +SVQNIKSVYKLSE DSTV+VLPLFHVHGL+AGLLSSL+AGAAV LPAAGRFSASTF
Sbjct: 187 NLASSVQNIKSVYKLSEMDSTVIVLPLFHVHGLLAGLLSSLVAGAAVALPAAGRFSASTF 246

Query: 245 WSDMIAYNATWYTAVPTIHQIILERHLSKPEPSYPKLRFIRSCSASLAPSILERLEQSFG 304
           W+DMI+YNATWYTAVPTIHQIIL+RHLSKPE +YPKLRFIRSCSASLAPSI+ RLE++F 
Sbjct: 247 WADMISYNATWYTAVPTIHQIILDRHLSKPEQTYPKLRFIRSCSASLAPSIMARLEEAFD 306

Query: 305 APVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENGAIQSEGVKGEVCI 364
           APVLE+YAMTEA+HLMASNPLPEDG HKAGSVG+P+GQEMAILDE+GAIQ+EGV GEVCI
Sbjct: 307 APVLEAYAMTEAAHLMASNPLPEDGPHKAGSVGRPVGQEMAILDEHGAIQAEGVSGEVCI 366

Query: 365 RGPNVTKGYKNNPDANNSAFMFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKISPIE 424
           RGPNVTKGYKNNP+AN  AF FGWFHTGD+GF DSDGYLHLVGRIKELINRGGEKISPIE
Sbjct: 367 RGPNVTKGYKNNPEANKQAFQFGWFHTGDLGFMDSDGYLHLVGRIKELINRGGEKISPIE 426

Query: 425 VDAVLLSHTDVSQAVAFGVPDNKYGEEINCAIIPREGSSINEADVLQFCKKNLASFKVPK 484
           VDAVLL+H DV+QAVAFGVPD+KYGEEINCA+IPREG  + E DV+Q+C+KNLASFKVPK
Sbjct: 427 VDAVLLAHPDVAQAVAFGVPDDKYGEEINCAVIPREGVVVTEQDVIQYCRKNLASFKVPK 486

Query: 485 KVFITDYLPKTASGKIQRRFVAEHFLA 511
           KVFITD +PKTASGKIQRR VAEHFLA
Sbjct: 487 KVFITDSVPKTASGKIQRRIVAEHFLA 513