Cucsa.302680 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.302680 vs. Swiss-Prot
Match: MOCOS_SOLLC (Molybdenum cofactor sulfurase OS=Solanum lycopersicum GN=FLACCA PE=2 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 8.8e-15
Identity = 77/336 (22.92%), Postives = 144/336 (42.86%), Query Frame = 1

Query: 65  SIPTLKDAFTYFIRAYPLYLDT-QQIDRIRADEYNHLALSKHVCLDYNGQCLFSFAQQQS 124
           +I + K+ F     +Y  Y ++ + ID IRA E+  L  +  V LD+ G  L+S +Q ++
Sbjct: 2   NIESEKEQFLKEFGSYYGYANSPKNIDEIRATEFKRL--NDTVYLDHAGATLYSESQMEA 61

Query: 125 SPMAPAASSSSPPGSPPLILHSPGSPFFNISHKAVKPNSQVKNGGQESEFESRIRSRIMK 184
                             +     S  +        P+SQ        +   + R +++ 
Sbjct: 62  ------------------VFKDLNSTLYG------NPHSQSTCSLATEDIVGKARQQVLS 121

Query: 185 FMNLSEDDYAMVFTANQSSAFKLLADTYPFQQNRNLITVYDHESEAVDLMVESSRKKGAR 244
           F N S  +Y+ +FT+  ++A KL+ +T+P+  N + +   ++ +  + +  E +  KGA 
Sbjct: 122 FFNASPREYSCIFTSGATAALKLVGETFPWSSNSSFMYSMENHNSVLGIR-EYALSKGAA 181

Query: 245 IYSAEFLWPNLNISTG-----KLRRLIVSKRKRKKKMK--MKMNKRGLFVLPLQSRLTGT 304
            ++ +    ++  S       KL +  + +R     +K  M  N   LF  P +   +G 
Sbjct: 182 AFAVDIEDTHVGESESPQSNLKLTQHHIQRRNEGGVLKEGMTGNTYNLFAFPSECNFSGR 241

Query: 305 PYSYQWLNIARDNE-------------WDVCLDT---CALGPKDMETLGLSLFKPEFLIS 364
            +    + I ++               W V +D    CA  P +     LS+FK +F++ 
Sbjct: 242 KFDPNLIKIIKEGSERILESSQYSRGCWLVLIDAAKGCATNPPN-----LSMFKADFVVF 301

Query: 365 SFYKVFGENPSGFGCLFIKKSNVSLMESLLTSPANI 377
           SFYK+FG  P+G G L ++K    LM+    S   +
Sbjct: 302 SFYKLFG-YPTGLGALIVRKDAAKLMKKTYFSGGTV 304

BLAST of Cucsa.302680 vs. Swiss-Prot
Match: MOCOS_CAEEL (Molybdenum cofactor sulfurase OS=Caenorhabditis elegans GN=mocs-1 PE=3 SV=2)

HSP 1 Score: 77.4 bits (189), Expect = 6.3e-13
Identity = 53/208 (25.48%), Postives = 94/208 (45.19%), Query Frame = 1

Query: 160 PNSQVKNGGQESEFESRIRSRIMKFMNLSEDDYAMVFTANQSSAFKLLADTYPFQQNRNL 219
           P+S      +  +  +  R RI+++ N + DDY +V T N +   K++A+ + F Q  + 
Sbjct: 32  PHSHHATAVKTKQIVNSARLRILQYFNTTSDDYFVVLTNNTTHGLKIVAENFKFGQKTHS 91

Query: 220 ITVYDHESEAVDLMVESSRKKGARIYSAEFLWPNLNISTGKLRRLIVSKRKRKKKMKMKM 279
           I           ++   S   G    S   +    ++  GK+  +     +   + ++  
Sbjct: 92  IL------NIASVLHGGSSNLGYLYDSHHSVVGLRHVVNGKVNSISCVNEESILEHEIPD 151

Query: 280 NKRGLFVLPLQSRLTGTPYSYQWLNIARDNEWDVCLDTCALGPKDMETLGLSLFKPEFLI 339
            +  LFVL   S   G  YS + ++  ++  W VCLD  +        L LS  +P F+ 
Sbjct: 152 VEHSLFVLTAMSNFCGKKYSLESVHRLQEKGWAVCLDAASF--VSSSALDLSQQRPNFIA 211

Query: 340 SSFYKVFGENPSGFGCLFIKKSNVSLME 368
            SFYK+FG  P+G G L ++K +  L+E
Sbjct: 212 FSFYKIFG-YPTGIGALLVRKDSAHLIE 230

BLAST of Cucsa.302680 vs. Swiss-Prot
Match: MOCOS_ARATH (Molybdenum cofactor sulfurase OS=Arabidopsis thaliana GN=ABA3 PE=1 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 9.1e-12
Identity = 56/243 (23.05%), Postives = 111/243 (45.68%), Query Frame = 1

Query: 160 PNSQVKNGGQESEFESRIRSRIMKFMNLSEDDYAMVFTANQSSAFKLLADTYPFQQNRNL 219
           P+SQ       S+  +  R +++++ N S +DY+ +FT+  ++A KL+ +T+P+ Q+ N 
Sbjct: 67  PHSQSDISSATSDLIADARHQVLEYFNASPEDYSCLFTSGATAALKLVGETFPWTQDSNF 126

Query: 220 ITVYDHESEAVDLMVESSRKKGARIYSAEF----LWPNLNISTG---KLRRLIVSKRKRK 279
           +   ++ +  + +  E +  +GA   + +       P    ++G   K++   V  R   
Sbjct: 127 LYTMENHNSVLGIR-EYALAQGASACAVDIEEAANQPGQLTNSGPSIKVKHRAVQMRNTS 186

Query: 280 K--KMKMKMNKRGLFVLPLQSRLTGTPYSYQWLNIARDN--------------EWDVCLD 339
           K  K + + N   LF  P +   +G  ++   + + ++N               W V +D
Sbjct: 187 KLQKEESRGNAYNLFAFPSECNFSGLRFNLDLVKLMKENTETVLQGSPFSKSKRWMVLID 246

Query: 340 T---CALGPKDMETLGLSLFKPEFLISSFYKVFGENPSGFGCLFIKKSNVSLMESLLTSP 377
               CA  P D     LS +  +F++ SFYK+FG  P+G G L ++     L++    S 
Sbjct: 247 AAKGCATLPPD-----LSEYPADFVVLSFYKLFG-YPTGLGALLVRNDAAKLLKKTYFSG 302

BLAST of Cucsa.302680 vs. Swiss-Prot
Match: MOCO1_CULQU (Molybdenum cofactor sulfurase 1 OS=Culex quinquefasciatus GN=mal1 PE=3 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 7.7e-11
Identity = 52/225 (23.11%), Postives = 100/225 (44.44%), Query Frame = 1

Query: 169 QESEFESRIRSRIMKFMNLSEDDYAMVFTANQSSAFKLLADTYPFQQNRNLITVYDHESE 228
           Q  +    +R R+++F N    +Y+++FT+  +++ K++A+ + F+              
Sbjct: 61  QTGQLMDEVRRRVLRFFNTDSSEYSLIFTSGATASLKMVAENFTFR-------------- 120

Query: 229 AVDLMVESSRKKGARIYSAE----FLWPNLNISTGKLRRLIVSKRKRKKKMKMKMNKR-- 288
           A D    +   +GA +Y  +     L     + T ++  L      R  K+  + ++R  
Sbjct: 121 AAD---SAEGDEGAFVYLRDNHTSVLGMRAIVGTSRIHPLERENFVRHLKVSARSSQRKP 180

Query: 289 GLFVLPLQSRLTGTPYSYQWLNIARDN--------EWDVCLDTCALGPKDMETLGLSLFK 348
            L V P Q+      Y  + +   R+N        ++ VCLD  +    +   L L  +K
Sbjct: 181 SLVVFPAQNNFNAAKYPLELIEEIRENGLVGYDDDKFYVCLDVASFVSTNF--LDLDRYK 240

Query: 349 PEFLISSFYKVFGENPSGFGCLFIKKSNVSLMESLLTSPANIGVI 380
           P+F+  SFYK+FG  P+G G L I+K +  L++        I ++
Sbjct: 241 PDFVCMSFYKIFG-YPTGLGALLIRKGSEDLLDKKYYGGGTIQIV 265

BLAST of Cucsa.302680 vs. Swiss-Prot
Match: MOCOS_ORYSJ (Molybdenum cofactor sulfurase OS=Oryza sativa subsp. japonica GN=MCSU3 PE=2 SV=2)

HSP 1 Score: 70.1 bits (170), Expect = 1.0e-10
Identity = 71/353 (20.11%), Postives = 145/353 (41.08%), Query Frame = 1

Query: 89  IDRIRADEYNHLALSKHVCLDYNGQCLFSFAQQQSSPMAPAASSSSPPGSPPLILHSPGS 148
           +D +RA E+  L    +  LD+ G  L+S AQ                     +L    S
Sbjct: 25  VDEMRAAEFKRLEGMAY--LDHAGATLYSEAQMAD------------------VLKDLAS 84

Query: 149 PFFNISHKAVKPNSQVKNGGQESEFESRIRSRIMKFMNLSEDDYAMVFTANQSSAFKLLA 208
             +        P+SQ  +    S+  +  R +++K+ N S  +Y  +FT+  ++A KL+ 
Sbjct: 85  NVYG------NPHSQSDSSMAASDLVTAARHQVLKYFNASPREYKCIFTSGATAALKLVG 144

Query: 209 DTYPFQQNRNLITVYDHESEAVDLMVESSRKKGARIYSAEF-LWPNLNISTGKLRRLIVS 268
           + +P+ +    +   ++ +  + +  E +  KGA + + +     +L    G      +S
Sbjct: 145 ECFPWSRESCYMYTMENHNSVLGIR-EYALSKGATVLAVDVEEGADLAKDNGSYSLYKIS 204

Query: 269 KRKRKKKMK--------------MKMNKRGLFVLPLQSRLTGTPYSYQWLNIARD----- 328
           +R  +++ K              +  N   +F  P +   +G  +S   + + ++     
Sbjct: 205 RRTNQRRSKDVLSHNCQNGSLSDISGNNWNIFAFPSECNFSGQKFSLSLVKLIKEGKIPL 264

Query: 329 ---NEWDVCLDT---CALGPKDMETLGLSLFKPEFLISSFYKVFGENPSGFGCLFIKKSN 388
               +W V +D    CA  P +     L+++  +F++ SFYK+FG  P+G G L +K   
Sbjct: 265 QQQGKWMVLIDAAKGCATEPPN-----LTVYPADFVVCSFYKIFG-YPTGLGALIVKNEA 324

Query: 389 VSLMESLLTSPANIGVITLISTSPSFPFTEEPETTETKTQQISKPTLEIQNLA 416
            +L+     S       T+ ++     F ++ +  E   Q +   T+   N+A
Sbjct: 325 ANLLNKTYFSGG-----TVAASIADIDFVQKRKNIE---QVLEDGTISFLNIA 336

BLAST of Cucsa.302680 vs. TrEMBL
Match: A0A0A0KKN1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G156130 PE=4 SV=1)

HSP 1 Score: 1285.0 bits (3324), Expect = 0.0e+00
Identity = 640/640 (100.00%), Postives = 640/640 (100.00%), Query Frame = 1

Query: 1   MQSPCIREASQACLRGCCRTPFLGLTDSSQTAIDRSSAASTPAYNFHGTTETSLHPDARF 60
           MQSPCIREASQACLRGCCRTPFLGLTDSSQTAIDRSSAASTPAYNFHGTTETSLHPDARF
Sbjct: 1   MQSPCIREASQACLRGCCRTPFLGLTDSSQTAIDRSSAASTPAYNFHGTTETSLHPDARF 60

Query: 61  SDHESIPTLKDAFTYFIRAYPLYLDTQQIDRIRADEYNHLALSKHVCLDYNGQCLFSFAQ 120
           SDHESIPTLKDAFTYFIRAYPLYLDTQQIDRIRADEYNHLALSKHVCLDYNGQCLFSFAQ
Sbjct: 61  SDHESIPTLKDAFTYFIRAYPLYLDTQQIDRIRADEYNHLALSKHVCLDYNGQCLFSFAQ 120

Query: 121 QQSSPMAPAASSSSPPGSPPLILHSPGSPFFNISHKAVKPNSQVKNGGQESEFESRIRSR 180
           QQSSPMAPAASSSSPPGSPPLILHSPGSPFFNISHKAVKPNSQVKNGGQESEFESRIRSR
Sbjct: 121 QQSSPMAPAASSSSPPGSPPLILHSPGSPFFNISHKAVKPNSQVKNGGQESEFESRIRSR 180

Query: 181 IMKFMNLSEDDYAMVFTANQSSAFKLLADTYPFQQNRNLITVYDHESEAVDLMVESSRKK 240
           IMKFMNLSEDDYAMVFTANQSSAFKLLADTYPFQQNRNLITVYDHESEAVDLMVESSRKK
Sbjct: 181 IMKFMNLSEDDYAMVFTANQSSAFKLLADTYPFQQNRNLITVYDHESEAVDLMVESSRKK 240

Query: 241 GARIYSAEFLWPNLNISTGKLRRLIVSKRKRKKKMKMKMNKRGLFVLPLQSRLTGTPYSY 300
           GARIYSAEFLWPNLNISTGKLRRLIVSKRKRKKKMKMKMNKRGLFVLPLQSRLTGTPYSY
Sbjct: 241 GARIYSAEFLWPNLNISTGKLRRLIVSKRKRKKKMKMKMNKRGLFVLPLQSRLTGTPYSY 300

Query: 301 QWLNIARDNEWDVCLDTCALGPKDMETLGLSLFKPEFLISSFYKVFGENPSGFGCLFIKK 360
           QWLNIARDNEWDVCLDTCALGPKDMETLGLSLFKPEFLISSFYKVFGENPSGFGCLFIKK
Sbjct: 301 QWLNIARDNEWDVCLDTCALGPKDMETLGLSLFKPEFLISSFYKVFGENPSGFGCLFIKK 360

Query: 361 SNVSLMESLLTSPANIGVITLISTSPSFPFTEEPETTETKTQQISKPTLEIQNLAIPESR 420
           SNVSLMESLLTSPANIGVITLISTSPSFPFTEEPETTETKTQQISKPTLEIQNLAIPESR
Sbjct: 361 SNVSLMESLLTSPANIGVITLISTSPSFPFTEEPETTETKTQQISKPTLEIQNLAIPESR 420

Query: 421 NSPEITEATEIEEEELSITGIVESTTPFVSTRSTNTEMNSYMDCRGLDHADSVGLRLISI 480
           NSPEITEATEIEEEELSITGIVESTTPFVSTRSTNTEMNSYMDCRGLDHADSVGLRLISI
Sbjct: 421 NSPEITEATEIEEEELSITGIVESTTPFVSTRSTNTEMNSYMDCRGLDHADSVGLRLISI 480

Query: 481 RARYLINWLTNALMNLQHPNPEGRIAKALVRIYGPKIEINRGPAVAFNIFDWKGEKVDPA 540
           RARYLINWLTNALMNLQHPNPEGRIAKALVRIYGPKIEINRGPAVAFNIFDWKGEKVDPA
Sbjct: 481 RARYLINWLTNALMNLQHPNPEGRIAKALVRIYGPKIEINRGPAVAFNIFDWKGEKVDPA 540

Query: 541 MVQKLADRSNISLSNGIVKEVSFLDKNEEENEMRKERAMEEGERIDRNEKRHCRIRVVSA 600
           MVQKLADRSNISLSNGIVKEVSFLDKNEEENEMRKERAMEEGERIDRNEKRHCRIRVVSA
Sbjct: 541 MVQKLADRSNISLSNGIVKEVSFLDKNEEENEMRKERAMEEGERIDRNEKRHCRIRVVSA 600

Query: 601 GIGFLTNFEDVYKFWAFVSRFLDADFVEKERWRYMALNQK 641
           GIGFLTNFEDVYKFWAFVSRFLDADFVEKERWRYMALNQK
Sbjct: 601 GIGFLTNFEDVYKFWAFVSRFLDADFVEKERWRYMALNQK 640

BLAST of Cucsa.302680 vs. TrEMBL
Match: M5WBZ1_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa017747mg PE=4 SV=1)

HSP 1 Score: 670.6 bits (1729), Expect = 1.9e-189
Identity = 355/653 (54.36%), Postives = 463/653 (70.90%), Query Frame = 1

Query: 1   MQSPCIREASQACLRGCCRTP-FLGLTDSSQTAIDRSSAASTPA------YNFHGTTETS 60
           M SPCIREAS+ CL  CC  P FLG   SS +    +   ST        Y F   T +S
Sbjct: 1   MLSPCIREASETCLHDCCPAPNFLGNHGSSTSNPSSTPNKSTETVVTGFRYAFTIATASS 60

Query: 61  LHPDARFSDHESIPTLKDAFTYFIRAYPLYLDTQQIDRIRADEYNHLALSKHVCLDYNGQ 120
           L PD +F++HES+P+L+++++YFI+AYP +  T Q D IRA EY HL LS HVCLDY G 
Sbjct: 61  LCPDTQFTNHESLPSLQESYSYFIQAYPQFSQTDQADHIRAHEYYHLTLSNHVCLDYIGH 120

Query: 121 CLFSFAQQQSSPMAP----AASSSSPPGSPPLILHSPGSPFFNISHKAVKPNSQVKNGGQ 180
            LFS++QQQ+    P    A++SSSPP  PP +LHSP   FF+IS+K+V  ++QV  GGQ
Sbjct: 121 GLFSYSQQQTQHYYPTPTIASTSSSPPPPPPQLLHSPEPLFFDISYKSVNLHTQVVYGGQ 180

Query: 181 ESEFESRIRSRIMKFMNLSEDDYAMVFTANQSSAFKLLADTYPFQQNRNLITVYDHESEA 240
           ESE E  +R RIM +MN+SE DYAMVFTANQSSAFKLLAD+YPFQQN +L+TVYD++ EA
Sbjct: 181 ESEVEFEMRKRIMSYMNISECDYAMVFTANQSSAFKLLADSYPFQQNPSLLTVYDYKCEA 240

Query: 241 VDLMVESSRKKGARIYSAEFLWPNLNISTGKLRRLIVSKRKRKKKMKMKMNKRGLFVLPL 300
           VD+M ESS+KKG R+ SAEF WPN+ I + KLR+ I + +K +KK        GLFV PL
Sbjct: 241 VDVMTESSKKKGGRVMSAEFSWPNMRIQSRKLRKRIGNMKKTRKKP-------GLFVFPL 300

Query: 301 QSRLTGTPYSYQWLNIARDNEWDVCLDTCALGPKDMETLGLSLFKPEFLISSFYKVFGEN 360
           QSR+TG  YSY W++IA++N W V LD C+LGPKDM+TLGLSLF+P+FLI SF+KVFGEN
Sbjct: 301 QSRMTGARYSYMWMSIAQENGWHVLLDACSLGPKDMDTLGLSLFQPDFLICSFFKVFGEN 360

Query: 361 PSGFGCLFIKKSNVSLMESLLTSPANIGVITLISTSPSFPFTEEPETTETKTQQISKPTL 420
           PSGFGCLF+KKS+ S+++   T  ++IG+++L+  S    ++E+  + + +T +      
Sbjct: 361 PSGFGCLFVKKSSASVLKDS-TFASSIGIVSLVPASKPSEYSEDSISMDIETDKKQSKL- 420

Query: 421 EIQNLAIPESRNSPEITEATEIEEEELSITGIVESTTP--FVSTRSTNTEMNSYMDCRGL 480
                   E+  S EI E T I+++  S++ I++      F S++  + E+    +CRGL
Sbjct: 421 --------ENSKSHEIEEVT-IKQKAPSLSEIMKLDRDHHFESSQPKSAEI----ECRGL 480

Query: 481 DHADSVGLRLISIRARYLINWLTNALMNLQHPNPEGRIAKALVRIYGPKIEINRGPAVAF 540
           DHADS+GL LIS RARYLINWL NALM+LQHP+ +      LVRIYGPKI++ RGP++AF
Sbjct: 481 DHADSLGLVLISRRARYLINWLVNALMSLQHPHSQ--YGHRLVRIYGPKIKVERGPSLAF 540

Query: 541 NIFDWKGEKVDPAMVQKLADRSNISLSNGIVKEVSFLDKNEEENEMRKERAMEEGERIDR 600
           N+FDWKGEK+DP +VQKLADR+NISLSNGI+  + F DK+EEE E + E    +     R
Sbjct: 541 NVFDWKGEKIDPLIVQKLADRNNISLSNGILNHIWFSDKHEEERETKLETCASDRLVNKR 600

Query: 601 NEKRHCRIRVVSAGIGFLTNFEDVYKFWAFVSRFLDADFVEKERWRYMALNQK 641
            +  H  I VV+A +GFLTNFED+Y+ WAFVSRFLDADFVEKERWRYMALNQ+
Sbjct: 601 KDGCHSGISVVTAALGFLTNFEDIYRLWAFVSRFLDADFVEKERWRYMALNQR 629

BLAST of Cucsa.302680 vs. TrEMBL
Match: B9RC09_RICCO (Molybdopterin cofactor sulfurase, putative OS=Ricinus communis GN=RCOM_1683160 PE=4 SV=1)

HSP 1 Score: 652.9 bits (1683), Expect = 4.0e-184
Identity = 343/659 (52.05%), Postives = 460/659 (69.80%), Query Frame = 1

Query: 1   MQSPCIREASQACLRGCCRTPFLGLTDSSQTAIDRSSAASTPAYNFHGTTETSLHPDARF 60
           M SPCIREAS+ C  GCC TPFLG     QTA   ++AAS+  Y+F     +S++P+++F
Sbjct: 1   MHSPCIREASEVCSHGCCPTPFLGFPQP-QTATSATTAASS-RYDFEVAMTSSIYPNSQF 60

Query: 61  SDHESIPTLKDAFTYFIRAYPLYLDTQQIDRIRADEYNHLALSKHVCLDYNGQCLFSFAQ 120
           ++HES+P+L ++F+ F +A+P Y  T   D+IRA EY HL+LS HVCLDY G  LFS++Q
Sbjct: 61  TNHESLPSLDESFSNFTKAFPQYALTDLADKIRAQEYYHLSLSNHVCLDYIGHGLFSYSQ 120

Query: 121 QQSSPMA-PAASSSSPPGSPPLILHSPG--SPFFNISHKAVKPNSQVKNGGQESEFESRI 180
           Q S   A P AS+S+ P  PP   HS     PFF+I +++V  NSQ++ GG ES+ E++I
Sbjct: 121 QASHYQASPIASTSTSP--PPSTSHSTALEPPFFDIFNRSVTLNSQLQYGGPESDMENKI 180

Query: 181 RSRIMKFMNLSEDDYAMVFTANQSSAFKLLADTYPFQQNRNLITVYDHESEAVDLMVESS 240
           R RI+ FMN+SED+Y +VFTANQ+SAFKLLAD YPFQ +R L+T+YD+ESEAV +M+ESS
Sbjct: 181 RRRIIAFMNISEDEYTVVFTANQTSAFKLLADAYPFQSHRKLLTMYDNESEAVKVMIESS 240

Query: 241 RKKGARIYSAEFLWPNLNISTGKLRRLIVSKRKRKKKMKMKMNKRGLFVLPLQSRLTGTP 300
           ++KG +++SA+F WP+L I +GKL++ +VSKRK ++K      KRGLFV PLQSR+TGT 
Sbjct: 241 KQKGGQVFSADFSWPSLRIQSGKLKKKVVSKRKTERK-----KKRGLFVFPLQSRMTGTR 300

Query: 301 YSYQWLNIARDNEWDVCLDTCALGPKDMETLGLSLFKPEFLISSFYKVFGENPSGFGCLF 360
           YSY W+++A++N W + LD CALGPK+METLGLSLFKP+FLI SF+KVFGENPSGFGCLF
Sbjct: 301 YSYFWMSMAQENGWHILLDACALGPKEMETLGLSLFKPDFLICSFFKVFGENPSGFGCLF 360

Query: 361 IKKSNVSLMESLLTSPANIGVITLISTSPSFPFTEEPETTETKTQQISKPTLEIQNLAIP 420
           +KKS+ S++ +  T+ A+IG++ L+       F+EE    + + +   K  LE+ N  I 
Sbjct: 361 VKKSSASVLMNSTTA-ASIGIVRLVPAIGPSQFSEESFVADVEIEP--KENLELHNDKIL 420

Query: 421 ESRNSPEI--------------TEATEIEEEELSITGIVESTTPFVSTRSTNTEMN--SY 480
           +  +S                 TE T I+++E  I  +    T F   +   +  N  + 
Sbjct: 421 QGMSSKPASGHQMSSRSSEMNETEETTIKQKESEIEELETPPTEFSQFKFNESGGNGKTV 480

Query: 481 MDCRGLDHADSVGLRLISIRARYLINWLTNALMNLQHPNPEGRIAKALVRIYGPKIEINR 540
           ++ +GL+HADS+GL LIS RARYLINWL NALM+LQHP+ E      L+RIYGPKI+ +R
Sbjct: 481 LEFKGLEHADSLGLILISTRARYLINWLVNALMSLQHPHSEN--GNPLIRIYGPKIKFDR 540

Query: 541 GPAVAFNIFDWKGEKVDPAMVQKLADRSNISLSNGIVKEVSFLDKNEEENEMRKERAMEE 600
           GPAVAFNIFDWKGE++DP +VQKLADR+NISLS G +  +    K+EE+     E   + 
Sbjct: 541 GPAVAFNIFDWKGERIDPVLVQKLADRNNISLSYGFLHHIWLPAKHEEQRGQLSEMGAQN 600

Query: 601 GERIDRNEKRHCRIRVVSAGIGFLTNFEDVYKFWAFVSRFLDADFVEKERWRYMALNQK 641
                  +K H  I  ++A +GFLTNFEDVY+ WAFVSRFLDADFVEKERWRY ALNQK
Sbjct: 601 LNEKREKQKPHSGISAITATLGFLTNFEDVYRLWAFVSRFLDADFVEKERWRYTALNQK 645

BLAST of Cucsa.302680 vs. TrEMBL
Match: F6HUI5_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_02s0025g04570 PE=4 SV=1)

HSP 1 Score: 652.1 bits (1681), Expect = 6.9e-184
Identity = 347/660 (52.58%), Postives = 447/660 (67.73%), Query Frame = 1

Query: 1   MQSPCIREASQACLRGCCRTPFLGLTDSSQTAIDRSSAASTPAYNFHGTTETSLHPDARF 60
           M SPCIRE S+AC +GCC     G  D   T     S+A+   YNF  TT +SL P+ +F
Sbjct: 1   MHSPCIRETSEACFQGCCLASLPGFPDPHGTDPKNLSSAAVSRYNFALTTVSSLFPNTQF 60

Query: 61  SDHESIPTLKDAFTYFIRAYPLYLDTQQIDRIRADEYNHLALSKHVCLDYNGQCLFSFAQ 120
           ++HES+P L ++F+ F +AYP Y +T Q D+IRA EY HL++S HVCLDY G  LFS++Q
Sbjct: 61  TNHESLPPLDESFSSFNKAYPQYSNTNQADQIRAQEYYHLSMSNHVCLDYIGHGLFSYSQ 120

Query: 121 QQSSPMAPAASSSSPPGSPPLILHSPGSPFFNISHKAVKPNSQVKNGGQESEFESRIRSR 180
            Q  P                        FF IS+K+V  NSQ+  GG+ESE ES+IR R
Sbjct: 121 LQKLP------------------------FFEISYKSVNLNSQILYGGEESELESKIRKR 180

Query: 181 IMKFMNLSEDDYAMVFTANQSSAFKLLADTYPFQQNRNLITVYDHESEAVDLMVESSRKK 240
           IM FMN+SE DY+MVFTANQSSAFKLLAD YPFQ N+NL+TVYD+E+EAV  M+ +S+K+
Sbjct: 181 IMDFMNISEADYSMVFTANQSSAFKLLADFYPFQSNQNLLTVYDYENEAVGAMIRASKKR 240

Query: 241 GARIYSAEFLWPNLNISTGKLRRLIVSKRKRKKKMKMKMNKRGLFVLPLQSRLTGTPYSY 300
            AR+ SAEF WPNL I + KL+++I++KRK+         +RGLFV PLQSR+TG  YSY
Sbjct: 241 SARVLSAEFSWPNLRIHSAKLKKIILNKRKK---------RRGLFVFPLQSRMTGARYSY 300

Query: 301 QWLNIARDNEWDVCLDTCALGPKDMETLGLSLFKPEFLISSFYKVFGENPSGFGCLFIKK 360
            W+++A++N W V LD CALGPKDMETLGLSLF+P+FLI SF+KVFG+NPSGFGCLF+KK
Sbjct: 301 LWMSMAQENGWHVLLDACALGPKDMETLGLSLFRPDFLICSFFKVFGKNPSGFGCLFVKK 360

Query: 361 SNVSLMESLLTSPANIGVITLISTSPSFPFTEEPETTETKTQQISK--------PTLEIQ 420
           S+ S+++   T+  ++G+++L+  +    F +E  TT+ +T+Q SK        P     
Sbjct: 361 SSASILKD-STTAVSVGIVSLLPATRRSQFPDESATTDIETEQTSKLKLHKGELPAASSL 420

Query: 421 NLAIPESRNSPEITEATEIEEEELSITG-----IVESTTPFVSTRSTNTEMN----SYMD 480
           +  +P  + S E  E+ EI +      G     IVE   P    +S N + +    S ++
Sbjct: 421 SGPLPVQKISNETFESYEISDVNFKQKGSSSSEIVELEMPLDIPQSLNKDSSVNGYSQIE 480

Query: 481 CRGLDHADSVGLRLISIRARYLINWLTNALMNLQHPNPEGRIAKALVRIYGPKIEINRGP 540
           CRGLDHADS+GL LIS+RAR+LINWL NALM+L+HP+ E  +   LVRIYGP +  +RGP
Sbjct: 481 CRGLDHADSLGLILISLRARFLINWLVNALMSLRHPHSENGL--PLVRIYGPNVAFDRGP 540

Query: 541 AVAFNIFDWKGEKVDPAMVQKLADRSNISLSNGIVKEVSFLDKNEEENEMRKERAMEEGE 600
           AVAFN+FDWKGEKV+P +VQKLADRSNISLS+G ++ + F DK EEE E   E      E
Sbjct: 541 AVAFNVFDWKGEKVEPTLVQKLADRSNISLSHGFLQHIWFSDKYEEEKEKILELRTIGVE 600

Query: 601 RIDRNEKR---HCRIRVVSAGIGFLTNFEDVYKFWAFVSRFLDADFVEKERWRYMALNQK 641
               N+KR      I VVSA +G LTNFEDVY  WAFVSRFLDADFVEKERWRY+ALNQK
Sbjct: 601 GTLGNKKRDKSSSGISVVSAALGLLTNFEDVYNLWAFVSRFLDADFVEKERWRYVALNQK 624

BLAST of Cucsa.302680 vs. TrEMBL
Match: A0A061G5C0_THECC (Pyridoxal phosphate-dependent transferases superfamily protein OS=Theobroma cacao GN=TCM_016164 PE=4 SV=1)

HSP 1 Score: 650.6 bits (1677), Expect = 2.0e-183
Identity = 356/665 (53.53%), Postives = 464/665 (69.77%), Query Frame = 1

Query: 1   MQSPCIREASQACLRGCCRTPFLGLTDS--SQTAIDRSSAASTPAYNFHGTTETSLHPDA 60
           M SPC+REASQAC  GCC  PF GL +S  + + I RS+AAS   Y F   T +SL P+ 
Sbjct: 1   MHSPCLREASQACY-GCCLNPFPGLPESRAATSQIPRSAAASR--YEFEVCTASSLCPNF 60

Query: 61  RFSDHESIPTLKDAFTYFIRAYPLYLDTQQIDRIRADEYNHLALSKHVCLDYNGQCLFSF 120
           +F++HES+P+ +++F+YFI+ YP Y  T Q D+IRA EY HL+LSKHVCLDY G  LFS+
Sbjct: 61  QFTNHESLPSSEESFSYFIKVYPQYSQTDQADKIRAQEYYHLSLSKHVCLDYIGHGLFSY 120

Query: 121 AQQQSS-PMAPAASSSS---PPGSPPLILHSPGSPFFNISHKAVKPNSQVKNGGQESEFE 180
           +Q +S  P +PAASSSS   PP  PP+   +  +PFF++S+K+V  NSQ+  GG+ESEFE
Sbjct: 121 SQLESQCPGSPAASSSSSPPPPPPPPVRSVTLEAPFFDVSYKSVNLNSQILYGGEESEFE 180

Query: 181 SRIRSRIMKFMNLSEDDYAMVFTANQSSAFKLLADTYPFQQNRNLITVYDHESEAVDLMV 240
           S IR RIM FMN+SE DY MV +ANQSSA KLLA++YPFQ  +NL+TVYD++SEAV++M+
Sbjct: 181 SNIRKRIMAFMNISEADYTMVLSANQSSASKLLAESYPFQSYQNLLTVYDYQSEAVEVMI 240

Query: 241 ESSRKKGARIYSAEFLWPNLNISTGKLRRLIVSKRKRKKKMKMKMNKRGLFVLPLQSRLT 300
           ESS+K+GA + SA F WPNL+I + KLR+ I +K K KKK        GLFV PLQSR+T
Sbjct: 241 ESSKKRGANVMSANFSWPNLSIQSEKLRKKIANKSKHKKK--------GLFVFPLQSRVT 300

Query: 301 GTPYSYQWLNIARDNEWDVCLDTCALGPKDMETLGLSLFKPEFLISSFYKVFGENPSGFG 360
           G+ YSY W+++A++N W V LD  ALG KDMETLGLSLF P+FLI SF+KVFGENPSGF 
Sbjct: 301 GSRYSYLWMSLAQENGWHVLLDASALGAKDMETLGLSLFNPDFLICSFFKVFGENPSGFC 360

Query: 361 CLFIKKSNVSLMESLLTSPANIGVITLISTSPSFPFTEEPETTETKTQQISKPTLEIQ-- 420
           CLFI+KS+ S+++   T+  +IG++ L+   P    T  PE++   + +  K + E    
Sbjct: 361 CLFIRKSSASVLKD-STTATSIGIVNLV---PGSEPTRIPESSAISSIETRKKSKEFPAQ 420

Query: 421 -----NLAIPESRNSPEI----TEATEIEEEELSITGIVE-STTPFVSTRS----TNTEM 480
                 ++I + R+   +    TE    +++ +S + I E   T F S  S    T    
Sbjct: 421 GSFSGPISIQQRRDETTLDLHKTEGINRKQKTVSFSEIEEVIETSFESASSIINNTRQSK 480

Query: 481 NSYMDCRGLDHADSVGLRLISIRARYLINWLTNALMNLQHPNPEGRIAKALVRIYGPKIE 540
           N  ++CR LDHADS+GL LIS R R LINWL NALM+LQHP+ E  I    V+IYGPKI 
Sbjct: 481 NPKIECRSLDHADSLGLILISSRTRNLINWLVNALMSLQHPHSENGI--PAVKIYGPKIM 540

Query: 541 INRGPAVAFNIFDWKGEKVDPAMVQKLADRSNISLSNGIVKEVSFLDKNEEENEMRKERA 600
            +RGPAVAFN+FDWKGEK+DP +VQKLADR+NISLS G ++ + F DK+EEE E + E  
Sbjct: 541 FDRGPAVAFNVFDWKGEKIDPVLVQKLADRNNISLSIGFLQHIWFSDKHEEEKEKQLETR 600

Query: 601 MEEGERIDRNEKR---HCRIRVVSAGIGFLTNFEDVYKFWAFVSRFLDADFVEKERWRYM 641
             E E    ++KR   H  I VV+A +GFLTNFED+Y+ WAFVSRFLDADF+EKE+WRY 
Sbjct: 601 TSEAEEPVSSKKRDKFHSGISVVTAALGFLTNFEDIYRLWAFVSRFLDADFLEKEKWRYK 648

BLAST of Cucsa.302680 vs. TAIR10
Match: AT4G22980.1 (AT4G22980.1 FUNCTIONS IN: molecular_function unknown)

HSP 1 Score: 424.9 bits (1091), Expect = 9.1e-119
Identity = 259/641 (40.41%), Postives = 365/641 (56.94%), Query Frame = 1

Query: 1   MQSPCIREASQACLRGCCRTPFLGLTDSSQTAIDRSSAASTPAYNFHGTTETSLHPDARF 60
           M S  I+EAS+AC  GCC +PF   + S +      S  +T      GT+   L  + +F
Sbjct: 1   MNSHFIQEASEACFNGCCSSPFSSHSMSEKPEELEFSVTTT------GTS--FLTRNTKF 60

Query: 61  SDHESIPTLKDAFTYFIRAYPLYLDTQQIDRIRADEYNHLALSKHVCLDYNGQCLFSFAQ 120
           +  ES+P L+ +F   I A+P YL T Q D +R+ EY +L+ S HV           F Q
Sbjct: 61  TSQESLPRLRTSFYDLITAFPDYLQTNQADHLRSTEYQNLSSSSHV-----------FGQ 120

Query: 121 QQSSPMAPAASSSSPPGSPPLILHSPGSPFFNISHKAVKPNSQVKNGGQESEFESRIRSR 180
           QQ  P+   +       S   + HS       +S K V    ++ +  +ES F+SRIR R
Sbjct: 121 QQ--PLFSYSQFREISESESDLNHS----LLTLSCKQVSSGKELLSFEEESRFQSRIRKR 180

Query: 181 IMKFMNLSEDDYAMVFTANQSSAFKLLADTYPFQQNRNLITVYDHESEAVDLMVESSRKK 240
           I  FMNL E +Y M+ T ++SSAFK++A+ Y F+ N NL+TVY++E EAV+ M+  S KK
Sbjct: 181 ITSFMNLEESEYHMILTQDRSSAFKIVAELYSFKTNPNLLTVYNYEDEAVEEMIRISEKK 240

Query: 241 GARIYSAEFLWPNLNISTGKLRRLIVSKRKRKKKMKMKMNKRGLFVLPLQSRLTGTPYSY 300
           G +  SAEF WP+  I + KL+R I   ++R         KRGLFV PLQS +TG  YSY
Sbjct: 241 GIKPQSAEFSWPSTEILSEKLKRRITRSKRR--------GKRGLFVFPLQSLVTGASYSY 300

Query: 301 QWLNIARDNEWDVCLDTCALGPKDMETLGLSLFKPEFLISSFYKVFG-ENPSGFGCLFIK 360
            W+++AR++EW V LDT ALG KDMETLGLSLF+P+FLI SF +V G ++PSGFGCLF+K
Sbjct: 301 SWMSLARESEWHVLLDTSALGSKDMETLGLSLFQPDFLICSFTEVLGQDDPSGFGCLFVK 360

Query: 361 KSNVSLMESLLTSPANIGVITLISTSPSFPFTEEPETTETKTQQISKPTLEIQNLAIPES 420
           KS+ + +    T+P N   +T +   PS+ +  E +                        
Sbjct: 361 KSSSTALSEEPTNPEN---LTAVKAEPSWKWKTEYQA----------------------- 420

Query: 421 RNSPEITEATEIEEEELSITGIVESTTPFVSTRSTNTEMNSYMDCRGLDHADSVGLRLIS 480
                  E T ++ E+        S++  V   S+  +  + ++ +GLDHADS+GL LIS
Sbjct: 421 ----GYNEITPVDHEDHKAAS--TSSSEIVEIESSVKQDKAMIEFQGLDHADSLGLILIS 480

Query: 481 IRARYLINWLTNALMNLQHPNPEGRIAKALVRIYGPKIEINRGPAVAFNIFDWKGEKVDP 540
            R++ L  WL  AL  LQHP    +    LV++YGPK + +RGP+++FNIFDW+GEKVDP
Sbjct: 481 RRSKSLTLWLLRALRTLQHPGYH-QTEMPLVKLYGPKTKPSRGPSISFNIFDWQGEKVDP 540

Query: 541 AMVQKLADRSNISLSNGIVKEVSFLDKNEEENEMRKERAMEEGERIDRNEKRHCRIRVVS 600
            MV++LA+R  I L        ++L K    N+ R + A+              R+ VV+
Sbjct: 541 LMVERLAEREKIGL------RCAYLHKFRIGNKRRSDEAVS------------LRLSVVT 557

Query: 601 AGI-GFLTNFEDVYKFWAFVSRFLDADFVEKERWRYMALNQ 640
             + GF+TNFEDV+K W FVSRFLDADFVEKE+WR  AL++
Sbjct: 601 VRLGGFMTNFEDVFKVWEFVSRFLDADFVEKEKWRMKALDK 557

BLAST of Cucsa.302680 vs. TAIR10
Match: AT5G51920.1 (AT5G51920.1 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein)

HSP 1 Score: 329.7 bits (844), Expect = 4.0e-90
Identity = 175/395 (44.30%), Postives = 256/395 (64.81%), Query Frame = 1

Query: 13  CLRGC-CRTPFLGLTDSSQTAIDRSSAAS-TPAYNFHGTTETSLHPDARFSDHESIPTLK 72
           CL GC   +PF G T S        +  S T   NF  TT +++ PD  F+D  S+P+ +
Sbjct: 14  CLHGCFSSSPFHGTTSSEHPPHSTPTVTSATLRRNFAQTTVSTIFPDTEFTDPNSLPSHQ 73

Query: 73  DAFTYFIRAYPLYLDTQQIDRIRADEYNHLALSKHVCLDYNGQCLFSFAQQQSSPMAPAA 132
           ++F+ FI+AYP Y DT +IDR+R+D Y HL LS + CLDY G  L+S++Q  +   +   
Sbjct: 74  ESFSDFIQAYPNYSDTYKIDRLRSDHYFHLGLSHYTCLDYIGIGLYSYSQLLNYDPSTYQ 133

Query: 133 SSSSPPGSPPLILHSPGSPFFNISHKAVKPNSQVKN-GGQESEFESRIRSRIMKFMNLSE 192
            SSS             SPFF++S K      ++ N GGQE+EFE  ++ RIM F+ +SE
Sbjct: 134 ISSS----------LSESPFFSVSPKIGNLKEKLLNDGGQETEFEYSMKRRIMGFLKISE 193

Query: 193 DDYAMVFTANQSSAFKLLADTYPFQQNRNLITVYDHESEAVDLMVESSRKKGARIYSAEF 252
           +DY+MVFTAN++SAF+L+A++YPF   R L+TVYD+ESEAV  +   S K+GA++ +AEF
Sbjct: 194 EDYSMVFTANRTSAFRLVAESYPFNSKRKLLTVYDYESEAVSEINRVSEKRGAKVAAAEF 253

Query: 253 LWPNLNISTGKLRRLIVSKRKRKKKMKMKMNKRGLFVLPLQSRLTGTPYSYQWLNIARDN 312
            WP L + + KLR+L+ + +   K       K+G++V PL SR+TG+ Y Y W+++A++N
Sbjct: 254 SWPRLKLCSSKLRKLVTAGKNGSKT-----KKKGIYVFPLHSRVTGSRYPYLWMSVAQEN 313

Query: 313 EWDVCLDTCALGPKDMETLGLSLFKPEFLISSFYKVFGENPSGFGCLFIKKSNVSLMESL 372
            W V +D C LGPKDM++ GLS++ P+F++ SFYKVFGENPSGFGCLF+KKS +S++ES 
Sbjct: 314 GWHVMIDACGLGPKDMDSFGLSIYNPDFMVCSFYKVFGENPSGFGCLFVKKSTISILES- 373

Query: 373 LTSPANIGVITLIST-SPSFPFTEEPETTETKTQQ 404
            T P   G+I L+ T +P      E   T+T +++
Sbjct: 374 STGP---GMINLVPTDNPISLHALEINRTQTDSEE 389

HSP 2 Score: 162.5 bits (410), Expect = 8.4e-40
Identity = 87/175 (49.71%), Postives = 117/175 (66.86%), Query Frame = 1

Query: 459 NSYMDCRGLDHADSVGLRLISIRARYLINWLTNALMNLQHPNPEGRIAKALVRIYGPKIE 518
           +S ++ +GLDH DS+GL     R+R LINWL +AL  L+H          LV+IYGPK+ 
Sbjct: 394 SSSVEYKGLDHVDSLGLVATGNRSRCLINWLVSALYKLKHST-----TSRLVKIYGPKVN 453

Query: 519 INRGPAVAFNIFDWKGEKVDPAMVQKLADRSNISLSNGIVKEVSFLDKNEEENEMRKERA 578
            NRGPAVAFN+F+ KGEK++P +VQKLA+ SNISL    +K + F    +E+ E  K+R 
Sbjct: 454 FNRGPAVAFNLFNHKGEKIEPFIVQKLAECSNISLGKSFLKNILF----QEDYEGVKDRV 513

Query: 579 MEEGERIDRNEKRHCRIRVVSAGIGFLTNFEDVYKFWAFVSRFLDADFVEKERWR 634
            E+    D +E    RI V++A +GFL NFEDVYK W FV+RFLD++FV+KE  R
Sbjct: 514 FEKKRNRDVDEP---RISVLTAALGFLANFEDVYKLWIFVARFLDSEFVDKESVR 556

BLAST of Cucsa.302680 vs. TAIR10
Match: AT5G66950.1 (AT5G66950.1 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein)

HSP 1 Score: 272.7 bits (696), Expect = 5.8e-73
Identity = 143/339 (42.18%), Postives = 217/339 (64.01%), Query Frame = 1

Query: 52  TSLHPDARFSDHESIPTLKDAFTYFIRAYPLYLDTQQIDRIRADEYNHLALSKHVCLDYN 111
           TSL     F   E++P L++A T F+  YP Y  ++++D +R DEY HL+L K VCLDY 
Sbjct: 94  TSLAAQRAFESEETLPELEEALTIFLTMYPKYQSSEKVDELRNDEYFHLSLPK-VCLDYC 153

Query: 112 GQCLFSFAQQQSSPMAPAASSSSPPGSPPLILHSPGSPFFNISH-KAVKPNSQVKNGGQE 171
           G  LFS+ Q                      +H   +  F++S   A   N  +  G ++
Sbjct: 154 GFGLFSYLQT---------------------VHYWDTCTFSLSEISANLSNHAIYGGAEK 213

Query: 172 SEFESRIRSRIMKFMNLSEDDYAMVFTANQSSAFKLLADTYPFQQNRNLITVYDHESEAV 231
              E  I+ RIM ++N+ E++Y +VFT ++ SAFKLLA++YPF  N+ L+T++DHES++V
Sbjct: 214 GSIEHDIKIRIMDYLNIPENEYGLVFTVSRGSAFKLLAESYPFHTNKKLLTMFDHESQSV 273

Query: 232 DLMVESSRKKGARIYSAEFLWPNLNISTGKLRRLIVSKRKRKKKMKMKMNKRGLFVLPLQ 291
             M + +++KGA++ SA F WP L + +  L++ I+SK+KRKK      +  GLFV P+Q
Sbjct: 274 SWMGQCAKEKGAKVGSAWFKWPTLRLCSMDLKKEILSKKKRKKD-----SATGLFVFPVQ 333

Query: 292 SRLTGTPYSYQWLNIARDNEWDVCLDTCALGPKDMETLGLSLFKPEFLISSFYKVFGENP 351
           SR+TG+ YSYQW+ +A+ N W V LD  ALGPKDM++LGLSLF+P+F+I+SFY+VFG +P
Sbjct: 334 SRVTGSKYSYQWMALAQQNNWHVLLDAGALGPKDMDSLGLSLFRPDFIITSFYRVFGYDP 393

Query: 352 SGFGCLFIKKSNVSLMESLLTSPANIGVITLISTSPSFP 390
           +GFGCL IKKS +S ++S  +   + G++ +   +P +P
Sbjct: 394 TGFGCLLIKKSVISCLQS-QSGKTSSGIVKI---TPEYP 401

HSP 2 Score: 133.3 bits (334), Expect = 5.5e-31
Identity = 72/169 (42.60%), Postives = 101/169 (59.76%), Query Frame = 1

Query: 464 CRGLDHADSVGLRLISIRARYLINWLTNALMNLQHPNPEGRIA-KALVRIYGPKIEINRG 523
           CR +DH + +GL   + R RYLINWL  +L+ L+ P  +     K LV+IYGPKI+  RG
Sbjct: 695 CRHIDHVNMLGLNKTTSRLRYLINWLVTSLLQLRLPRSDSDGEHKNLVQIYGPKIKYERG 754

Query: 524 PAVAFNIFDWKGEKVDPAMVQKLADRSNISLSNGIVKEVSFLDKNEEENEMRKERAMEEG 583
            +VAFNI D K   V P +VQKLA+R  ISL  G +  +  +D   E++   K       
Sbjct: 755 SSVAFNIRDLKSGMVHPEIVQKLAEREGISLGIGYLSHIKIIDNRSEDSSSWKP------ 814

Query: 584 ERIDRNEKRH--CRIRVVSAGIGFLTNFEDVYKFWAFVSRFLDADFVEK 630
             +DR  + +   R+ VV+A +GFLTNFEDVY+ W FV++FL   F ++
Sbjct: 815 --VDREGRNNGFIRVEVVTASLGFLTNFEDVYRLWNFVAKFLSPGFAKQ 855

BLAST of Cucsa.302680 vs. TAIR10
Match: AT4G37100.1 (AT4G37100.1 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein)

HSP 1 Score: 272.7 bits (696), Expect = 5.8e-73
Identity = 165/450 (36.67%), Postives = 253/450 (56.22%), Query Frame = 1

Query: 27  DSSQTAIDRSSAASTPAYNFHGTTE----TSLHPDARFSDHESIPTLKDAFTYFIRAYPL 86
           D  Q   D S   S      H   E    T+L  +      +SIP L++A T F+  YP 
Sbjct: 69  DIDQDNGDGSLGRSRSLARLHAQREFLRATALAAERIIESEDSIPELREALTKFLSMYPK 128

Query: 87  YLDTQQIDRIRADEYNHLALS-KHVCLDYNGQCLFSFAQQQSSPMAPAASSSSPPGSPPL 146
           Y  +++ID++R+DEY+HL+ S   VCLDY G  LFS+ Q                     
Sbjct: 129 YQASEKIDQLRSDEYSHLSSSASKVCLDYCGFGLFSYVQT-------------------- 188

Query: 147 ILHSPGSPFFNISHKAVKPNSQVKNGGQES-EFESRIRSRIMKFMNLSEDDYAMVFTANQ 206
            LH   +  F++S      ++    GG ES   E  I++RIM ++N+ E++Y +VFT ++
Sbjct: 189 -LHYWDTCTFSLSEITANLSNHALYGGAESGTVEHDIKTRIMDYLNIPENEYGLVFTVSR 248

Query: 207 SSAFKLLADTYPFQQNRNLITVYDHESEAVDLMVESSRKKGARIYSAEFLWPNLNISTGK 266
            SAF+LLA++YPFQ N+ L+T++DHES++V+ M +++R+KGA+ Y+A F WP L + +  
Sbjct: 249 GSAFRLLAESYPFQSNKRLLTMFDHESQSVNWMAQTAREKGAKAYNAWFKWPTLKLCSTD 308

Query: 267 LRRLIVSKRKRKKKMKMKMNKRGLFVLPLQSRLTGTPYSYQWLNIARDNEWDVCLDTCAL 326
           L++ +  K+++KK   +     GLFV P QSR+TGT YSYQW+ +A+ N W V LD  +L
Sbjct: 309 LKKRLSYKKRKKKDSAV-----GLFVFPAQSRVTGTKYSYQWMALAQQNHWHVLLDAGSL 368

Query: 327 GPKDMETLGLSLFKPEFLISSFYKVFGENPSGFGCLFIKKSNVSLMESLLTSPANIGVIT 386
           GPKDM++LGLSLF+PEF+I+SFY+VFG +P+GFGCL IKK   S+M SL +     G   
Sbjct: 369 GPKDMDSLGLSLFRPEFIITSFYRVFGHDPTGFGCLLIKK---SVMGSLQSQSGKTG-SG 428

Query: 387 LISTSPSFP------------FTEEPETTETKTQQISKPTLEIQNLAIPESRNSPEITEA 446
           ++  +P +P                 +  + KT++  +P  ++   A   +  S ++ + 
Sbjct: 429 IVKITPEYPLYLSDSVDGLDGLVGFEDHNDDKTKEAHRPGTQMP--AFSGAYTSAQVRDV 486

Query: 447 TEIEEEELSITGI---VESTTPFVSTRSTN 456
            E E  E +I+       STT F  T S +
Sbjct: 489 FETELLEDNISSDRDGTTSTTIFEETESVS 486

HSP 2 Score: 131.0 bits (328), Expect = 2.7e-30
Identity = 72/175 (41.14%), Postives = 100/175 (57.14%), Query Frame = 1

Query: 452 RSTNTEMNSYMDCRGLDHADSVGLRLISIRARYLINWLTNALMNLQHPNPEGRIAKALVR 511
           R T TE+     CR +DH + +GL   + R R+LINWL  +L+ LQ P   GR    LV+
Sbjct: 706 RDTETEIV----CRHIDHVNMLGLNKTTTRLRFLINWLVISLLQLQVPESGGRHMN-LVQ 765

Query: 512 IYGPKIEINRGPAVAFNIFDWKGEKVDPAMVQKLADRSNISLSNGIVKEVSFLDKNEEEN 571
           IYGPKI+  RG AVAFN+ D     V P +VQ+L DR  +SL  GI+  +  +D+    +
Sbjct: 766 IYGPKIKYERGAAVAFNVRDKSKGFVSPEIVQRLGDREGVSLGIGILSHIRIVDEKPRNH 825

Query: 572 EMRKERAMEEGERIDRNEKRHCRIRVVSAGIGFLTNFEDVYKFWAFVSRFLDADF 627
             R +       + +  +    R  VV+A + FLTNFEDVYK W FV++FL+  F
Sbjct: 826 RARTKEDSALHLQNEAGKNGFIRFEVVTASLSFLTNFEDVYKLWVFVAKFLNPGF 875

BLAST of Cucsa.302680 vs. TAIR10
Match: AT2G23520.1 (AT2G23520.1 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein)

HSP 1 Score: 265.8 bits (678), Expect = 7.0e-71
Identity = 144/361 (39.89%), Postives = 217/361 (60.11%), Query Frame = 1

Query: 34  DRSSAASTPAYNFHGTTE----TSLHPDARFSDHESIPTLKDAFTYFIRAYPLYLDTQQI 93
           D S   S      H   E    T+L  +  F   + IP L +AF  F+  YP +  ++++
Sbjct: 74  DESLGRSRSLARLHAQREFLRATALAAERAFESEDDIPELLEAFNKFLTMYPKFETSEKV 133

Query: 94  DRIRADEYNHLALSKHVCLDYNGQCLFSFAQQQSSPMAPAASSSSPPGSPPLILHSPGSP 153
           D++R+DEY HL  SK VCLDY G  LFS+ Q                      LH   S 
Sbjct: 134 DQLRSDEYGHLLDSK-VCLDYCGFGLFSYVQT---------------------LHYWDSC 193

Query: 154 FFNISHKAVKPNSQVKNGGQE-SEFESRIRSRIMKFMNLSEDDYAMVFTANQSSAFKLLA 213
            F++S      ++    GG E    E  +++RIM ++N+ E +Y +VFT ++ SAF+LLA
Sbjct: 194 TFSLSEITANLSNHALYGGAEIGTVEHDLKTRIMDYLNIPESEYGLVFTGSRGSAFRLLA 253

Query: 214 DTYPFQQNRNLITVYDHESEAVDLMVESSRKKGARIYSAEFLWPNLNISTGKLRRLIVSK 273
           ++YPF  N+ L+T++DHES++V+ M +++R+KGA+ Y+A F WP L + +  L++ +  K
Sbjct: 254 ESYPFHTNKRLLTMFDHESQSVNWMAQTAREKGAKAYNAWFKWPTLKLCSTDLKKRLSHK 313

Query: 274 RKRKKKMKMKMNKRGLFVLPLQSRLTGTPYSYQWLNIARDNEWDVCLDTCALGPKDMETL 333
           +++KK   +     GLFV P QSR+TG+ YSYQW+ +A+ N W V LD  +LGPKDM++L
Sbjct: 314 KRKKKDSAV-----GLFVFPAQSRVTGSKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSL 373

Query: 334 GLSLFKPEFLISSFYKVFGENPSGFGCLFIKKSNVSLMESLLTSPANIGVITLISTSPSF 390
           GLSLF+PEF+I+SFYKVFG +P+GFGCL IKK   S+M +L +     G   ++  +P +
Sbjct: 374 GLSLFRPEFIITSFYKVFGHDPTGFGCLLIKK---SVMGNLQSQSGKTG-SGIVKITPQY 403

HSP 2 Score: 119.8 bits (299), Expect = 6.2e-27
Identity = 70/175 (40.00%), Postives = 102/175 (58.29%), Query Frame = 1

Query: 464 CRGLDHADSVGLRLISIRARYLINWLTNALMNLQHPNPEGRIAKA---LVRIYGPKIEIN 523
           C  +DH + +GL   + R R+LINWL  +L+ L+ P P    +     LV+IYGPKI+  
Sbjct: 706 CSHIDHVNMLGLNKTTSRLRFLINWLVISLLQLKVPEPGSDGSSRYMNLVQIYGPKIKYE 765

Query: 524 RGPAVAFNIFDWKGEKVDPAMVQKLADRSNISLSNGIVKEVSFLD---------KNEEEN 583
           RG AVAFN+ D     V P +V KLA+R  +SL  GI+  +  +D         + +E++
Sbjct: 766 RGAAVAFNVKDKSKGFVSPEIVLKLAEREGVSLGIGILSHIRIMDLPRNHRGGARIKEDS 825

Query: 584 EMRKERAMEEGERIDRNEKRHCRIRVVSAGIGFLTNFEDVYKFWAFVSRFLDADF 627
            +  +R  E G+R  +N     R  VV+A + FL+NFEDVYK WAFV++FL+  F
Sbjct: 826 SLHLQR--EAGKRGGKN--GFVRFEVVTASLSFLSNFEDVYKLWAFVAKFLNPGF 876

BLAST of Cucsa.302680 vs. NCBI nr
Match: gi|778699798|ref|XP_004143996.2| (PREDICTED: molybdenum cofactor sulfurase-like [Cucumis sativus])

HSP 1 Score: 1285.0 bits (3324), Expect = 0.0e+00
Identity = 640/640 (100.00%), Postives = 640/640 (100.00%), Query Frame = 1

Query: 1   MQSPCIREASQACLRGCCRTPFLGLTDSSQTAIDRSSAASTPAYNFHGTTETSLHPDARF 60
           MQSPCIREASQACLRGCCRTPFLGLTDSSQTAIDRSSAASTPAYNFHGTTETSLHPDARF
Sbjct: 1   MQSPCIREASQACLRGCCRTPFLGLTDSSQTAIDRSSAASTPAYNFHGTTETSLHPDARF 60

Query: 61  SDHESIPTLKDAFTYFIRAYPLYLDTQQIDRIRADEYNHLALSKHVCLDYNGQCLFSFAQ 120
           SDHESIPTLKDAFTYFIRAYPLYLDTQQIDRIRADEYNHLALSKHVCLDYNGQCLFSFAQ
Sbjct: 61  SDHESIPTLKDAFTYFIRAYPLYLDTQQIDRIRADEYNHLALSKHVCLDYNGQCLFSFAQ 120

Query: 121 QQSSPMAPAASSSSPPGSPPLILHSPGSPFFNISHKAVKPNSQVKNGGQESEFESRIRSR 180
           QQSSPMAPAASSSSPPGSPPLILHSPGSPFFNISHKAVKPNSQVKNGGQESEFESRIRSR
Sbjct: 121 QQSSPMAPAASSSSPPGSPPLILHSPGSPFFNISHKAVKPNSQVKNGGQESEFESRIRSR 180

Query: 181 IMKFMNLSEDDYAMVFTANQSSAFKLLADTYPFQQNRNLITVYDHESEAVDLMVESSRKK 240
           IMKFMNLSEDDYAMVFTANQSSAFKLLADTYPFQQNRNLITVYDHESEAVDLMVESSRKK
Sbjct: 181 IMKFMNLSEDDYAMVFTANQSSAFKLLADTYPFQQNRNLITVYDHESEAVDLMVESSRKK 240

Query: 241 GARIYSAEFLWPNLNISTGKLRRLIVSKRKRKKKMKMKMNKRGLFVLPLQSRLTGTPYSY 300
           GARIYSAEFLWPNLNISTGKLRRLIVSKRKRKKKMKMKMNKRGLFVLPLQSRLTGTPYSY
Sbjct: 241 GARIYSAEFLWPNLNISTGKLRRLIVSKRKRKKKMKMKMNKRGLFVLPLQSRLTGTPYSY 300

Query: 301 QWLNIARDNEWDVCLDTCALGPKDMETLGLSLFKPEFLISSFYKVFGENPSGFGCLFIKK 360
           QWLNIARDNEWDVCLDTCALGPKDMETLGLSLFKPEFLISSFYKVFGENPSGFGCLFIKK
Sbjct: 301 QWLNIARDNEWDVCLDTCALGPKDMETLGLSLFKPEFLISSFYKVFGENPSGFGCLFIKK 360

Query: 361 SNVSLMESLLTSPANIGVITLISTSPSFPFTEEPETTETKTQQISKPTLEIQNLAIPESR 420
           SNVSLMESLLTSPANIGVITLISTSPSFPFTEEPETTETKTQQISKPTLEIQNLAIPESR
Sbjct: 361 SNVSLMESLLTSPANIGVITLISTSPSFPFTEEPETTETKTQQISKPTLEIQNLAIPESR 420

Query: 421 NSPEITEATEIEEEELSITGIVESTTPFVSTRSTNTEMNSYMDCRGLDHADSVGLRLISI 480
           NSPEITEATEIEEEELSITGIVESTTPFVSTRSTNTEMNSYMDCRGLDHADSVGLRLISI
Sbjct: 421 NSPEITEATEIEEEELSITGIVESTTPFVSTRSTNTEMNSYMDCRGLDHADSVGLRLISI 480

Query: 481 RARYLINWLTNALMNLQHPNPEGRIAKALVRIYGPKIEINRGPAVAFNIFDWKGEKVDPA 540
           RARYLINWLTNALMNLQHPNPEGRIAKALVRIYGPKIEINRGPAVAFNIFDWKGEKVDPA
Sbjct: 481 RARYLINWLTNALMNLQHPNPEGRIAKALVRIYGPKIEINRGPAVAFNIFDWKGEKVDPA 540

Query: 541 MVQKLADRSNISLSNGIVKEVSFLDKNEEENEMRKERAMEEGERIDRNEKRHCRIRVVSA 600
           MVQKLADRSNISLSNGIVKEVSFLDKNEEENEMRKERAMEEGERIDRNEKRHCRIRVVSA
Sbjct: 541 MVQKLADRSNISLSNGIVKEVSFLDKNEEENEMRKERAMEEGERIDRNEKRHCRIRVVSA 600

Query: 601 GIGFLTNFEDVYKFWAFVSRFLDADFVEKERWRYMALNQK 641
           GIGFLTNFEDVYKFWAFVSRFLDADFVEKERWRYMALNQK
Sbjct: 601 GIGFLTNFEDVYKFWAFVSRFLDADFVEKERWRYMALNQK 640

BLAST of Cucsa.302680 vs. NCBI nr
Match: gi|659073684|ref|XP_008437196.1| (PREDICTED: uncharacterized protein LOC103482698 [Cucumis melo])

HSP 1 Score: 1232.2 bits (3187), Expect = 0.0e+00
Identity = 613/640 (95.78%), Postives = 624/640 (97.50%), Query Frame = 1

Query: 1   MQSPCIREASQACLRGCCRTPFLGLTDSSQTAIDRSSAASTPAYNFHGTTETSLHPDARF 60
           MQSPCIREASQACLRGCCRTPFLGLTDSSQTAIDRSSAASTPAYNFHG TETSL P+ARF
Sbjct: 1   MQSPCIREASQACLRGCCRTPFLGLTDSSQTAIDRSSAASTPAYNFHGITETSLRPNARF 60

Query: 61  SDHESIPTLKDAFTYFIRAYPLYLDTQQIDRIRADEYNHLALSKHVCLDYNGQCLFSFAQ 120
           SDHESIP+LKDAFTYFIRAYPLYLDTQQIDRIRADEYNHLALSKHVCLDYNGQCLFSFAQ
Sbjct: 61  SDHESIPSLKDAFTYFIRAYPLYLDTQQIDRIRADEYNHLALSKHVCLDYNGQCLFSFAQ 120

Query: 121 QQSSPMAPAASSSSPPGSPPLILHSPGSPFFNISHKAVKPNSQVKNGGQESEFESRIRSR 180
           QQSSPM PAASSSSP GSPPLILHSPGSPFFNISHKAVKPNSQVKNGGQESEFESRIRSR
Sbjct: 121 QQSSPMTPAASSSSPSGSPPLILHSPGSPFFNISHKAVKPNSQVKNGGQESEFESRIRSR 180

Query: 181 IMKFMNLSEDDYAMVFTANQSSAFKLLADTYPFQQNRNLITVYDHESEAVDLMVESSRKK 240
           IMKFMNLSEDDYAMVFTANQSSAFKLLADTYPFQQNRNLITVYDH+SEAVDLMVESSRKK
Sbjct: 181 IMKFMNLSEDDYAMVFTANQSSAFKLLADTYPFQQNRNLITVYDHKSEAVDLMVESSRKK 240

Query: 241 GARIYSAEFLWPNLNISTGKLRRLIVSKRKRKKKMKMKMNKRGLFVLPLQSRLTGTPYSY 300
           GARIYSAEFLWPNLNISTGKLRRLIVSKRKRKKKMKM M KRGLFV PLQSRLTGTPYSY
Sbjct: 241 GARIYSAEFLWPNLNISTGKLRRLIVSKRKRKKKMKMTMKKRGLFVFPLQSRLTGTPYSY 300

Query: 301 QWLNIARDNEWDVCLDTCALGPKDMETLGLSLFKPEFLISSFYKVFGENPSGFGCLFIKK 360
           QWLNIARDNEWDVCLDTCALGPKDMETLGLSLFKPEFLISSFYKVFGENPSGFGCLFIKK
Sbjct: 301 QWLNIARDNEWDVCLDTCALGPKDMETLGLSLFKPEFLISSFYKVFGENPSGFGCLFIKK 360

Query: 361 SNVSLMESLLTSPANIGVITLISTSPSFPFTEEPETTETKTQQISKPTLEIQNLAIPESR 420
           SNVS MESLLTSPANIGVI+LISTSPSFPF EEPETTE +TQQISKPTLEIQ+LA PES 
Sbjct: 361 SNVSFMESLLTSPANIGVISLISTSPSFPFPEEPETTEIETQQISKPTLEIQSLARPESG 420

Query: 421 NSPEITEATEIEEEELSITGIVESTTPFVSTRSTNTEMNSYMDCRGLDHADSVGLRLISI 480
           NSPEITEATEIEEE LSITGIVESTTPFV+TRSTNTEMNSY+DCRGLDHADS+GLRLISI
Sbjct: 421 NSPEITEATEIEEEVLSITGIVESTTPFVATRSTNTEMNSYLDCRGLDHADSIGLRLISI 480

Query: 481 RARYLINWLTNALMNLQHPNPEGRIAKALVRIYGPKIEINRGPAVAFNIFDWKGEKVDPA 540
           R RYLINWLTNALMNLQHPNPEG IAKALVRIYGPKIEINRGPAVAFNIFDWKGEKVDPA
Sbjct: 481 RGRYLINWLTNALMNLQHPNPEGGIAKALVRIYGPKIEINRGPAVAFNIFDWKGEKVDPA 540

Query: 541 MVQKLADRSNISLSNGIVKEVSFLDKNEEENEMRKERAMEEGERIDRNEKRHCRIRVVSA 600
           MVQKLADRSNISLSNGIVKEVSFLDKNEEENEMRKERA+EEGE IDRNEKRHCRIRVVSA
Sbjct: 541 MVQKLADRSNISLSNGIVKEVSFLDKNEEENEMRKERALEEGEIIDRNEKRHCRIRVVSA 600

Query: 601 GIGFLTNFEDVYKFWAFVSRFLDADFVEKERWRYMALNQK 641
           GIGFLTNFEDVY+FWAFVSRFLDADFVEKERWRYMALNQK
Sbjct: 601 GIGFLTNFEDVYRFWAFVSRFLDADFVEKERWRYMALNQK 640

BLAST of Cucsa.302680 vs. NCBI nr
Match: gi|225426751|ref|XP_002275855.1| (PREDICTED: molybdenum cofactor sulfurase [Vitis vinifera])

HSP 1 Score: 672.9 bits (1735), Expect = 5.4e-190
Identity = 355/660 (53.79%), Postives = 456/660 (69.09%), Query Frame = 1

Query: 1   MQSPCIREASQACLRGCCRTPFLGLTDSSQTAIDRSSAASTPAYNFHGTTETSLHPDARF 60
           M SPCIRE S+AC +GCC     G  D   T     S+A+   YNF  TT +SL P+ +F
Sbjct: 1   MHSPCIRETSEACFQGCCLASLPGFPDPHGTDPKNLSSAAVSRYNFALTTVSSLFPNTQF 60

Query: 61  SDHESIPTLKDAFTYFIRAYPLYLDTQQIDRIRADEYNHLALSKHVCLDYNGQCLFSFAQ 120
           ++HES+P L ++F+ F +AYP Y +T Q D+IRA EY HL++S HVCLDY G  LFS++Q
Sbjct: 61  TNHESLPPLDESFSSFNKAYPQYSNTNQADQIRAQEYYHLSMSNHVCLDYIGHGLFSYSQ 120

Query: 121 QQSSPMAPAASSSSPPGSPPLILHSPGSPFFNISHKAVKPNSQVKNGGQESEFESRIRSR 180
            QS  M     SSS   +P L   S   PFF IS+K+V  NSQ+  GG+ESE ES+IR R
Sbjct: 121 LQSHHMTAPVPSSSSSSAPSLNFSSLELPFFEISYKSVNLNSQILYGGEESELESKIRKR 180

Query: 181 IMKFMNLSEDDYAMVFTANQSSAFKLLADTYPFQQNRNLITVYDHESEAVDLMVESSRKK 240
           IM FMN+SE DY+MVFTANQSSAFKLLAD YPFQ N+NL+TVYD+E+EAV  M+ +S+K+
Sbjct: 181 IMDFMNISEADYSMVFTANQSSAFKLLADFYPFQSNQNLLTVYDYENEAVGAMIRASKKR 240

Query: 241 GARIYSAEFLWPNLNISTGKLRRLIVSKRKRKKKMKMKMNKRGLFVLPLQSRLTGTPYSY 300
            AR+ SAEF WPNL I + KL+++I++KRK+         +RGLFV PLQSR+TG  YSY
Sbjct: 241 SARVLSAEFSWPNLRIHSAKLKKIILNKRKK---------RRGLFVFPLQSRMTGARYSY 300

Query: 301 QWLNIARDNEWDVCLDTCALGPKDMETLGLSLFKPEFLISSFYKVFGENPSGFGCLFIKK 360
            W+++A++N W V LD CALGPKDMETLGLSLF+P+FLI SF+KVFG+NPSGFGCLF+KK
Sbjct: 301 LWMSMAQENGWHVLLDACALGPKDMETLGLSLFRPDFLICSFFKVFGKNPSGFGCLFVKK 360

Query: 361 SNVSLMESLLTSPANIGVITLISTSPSFPFTEEPETTETKTQQISK--------PTLEIQ 420
           S+ S+++   T+  ++G+++L+  +    F +E  TT+ +T+Q SK        P     
Sbjct: 361 SSASILKD-STTAVSVGIVSLLPATRRSQFPDESATTDIETEQTSKLKLHKGELPAASSL 420

Query: 421 NLAIPESRNSPEITEATEIEEEELSITG-----IVESTTPFVSTRSTNTEMN----SYMD 480
           +  +P  + S E  E+ EI +      G     IVE   P    +S N + +    S ++
Sbjct: 421 SGPLPVQKISNETFESYEISDVNFKQKGSSSSEIVELEMPLDIPQSLNKDSSVNGYSQIE 480

Query: 481 CRGLDHADSVGLRLISIRARYLINWLTNALMNLQHPNPEGRIAKALVRIYGPKIEINRGP 540
           CRGLDHADS+GL LIS+RAR+LINWL NALM+L+HP+ E  +   LVRIYGP +  +RGP
Sbjct: 481 CRGLDHADSLGLILISLRARFLINWLVNALMSLRHPHSENGL--PLVRIYGPNVAFDRGP 540

Query: 541 AVAFNIFDWKGEKVDPAMVQKLADRSNISLSNGIVKEVSFLDKNEEENEMRKERAMEEGE 600
           AVAFN+FDWKGEKV+P +VQKLADRSNISLS+G ++ + F DK EEE E   E      E
Sbjct: 541 AVAFNVFDWKGEKVEPTLVQKLADRSNISLSHGFLQHIWFSDKYEEEKEKILELRTIGVE 600

Query: 601 RIDRNEKR---HCRIRVVSAGIGFLTNFEDVYKFWAFVSRFLDADFVEKERWRYMALNQK 641
               N+KR      I VVSA +G LTNFEDVY  WAFVSRFLDADFVEKERWRY+ALNQK
Sbjct: 601 GTLGNKKRDKSSSGISVVSAALGLLTNFEDVYNLWAFVSRFLDADFVEKERWRYVALNQK 648

BLAST of Cucsa.302680 vs. NCBI nr
Match: gi|595851117|ref|XP_007210050.1| (hypothetical protein PRUPE_ppa017747mg [Prunus persica])

HSP 1 Score: 670.6 bits (1729), Expect = 2.7e-189
Identity = 355/653 (54.36%), Postives = 463/653 (70.90%), Query Frame = 1

Query: 1   MQSPCIREASQACLRGCCRTP-FLGLTDSSQTAIDRSSAASTPA------YNFHGTTETS 60
           M SPCIREAS+ CL  CC  P FLG   SS +    +   ST        Y F   T +S
Sbjct: 1   MLSPCIREASETCLHDCCPAPNFLGNHGSSTSNPSSTPNKSTETVVTGFRYAFTIATASS 60

Query: 61  LHPDARFSDHESIPTLKDAFTYFIRAYPLYLDTQQIDRIRADEYNHLALSKHVCLDYNGQ 120
           L PD +F++HES+P+L+++++YFI+AYP +  T Q D IRA EY HL LS HVCLDY G 
Sbjct: 61  LCPDTQFTNHESLPSLQESYSYFIQAYPQFSQTDQADHIRAHEYYHLTLSNHVCLDYIGH 120

Query: 121 CLFSFAQQQSSPMAP----AASSSSPPGSPPLILHSPGSPFFNISHKAVKPNSQVKNGGQ 180
            LFS++QQQ+    P    A++SSSPP  PP +LHSP   FF+IS+K+V  ++QV  GGQ
Sbjct: 121 GLFSYSQQQTQHYYPTPTIASTSSSPPPPPPQLLHSPEPLFFDISYKSVNLHTQVVYGGQ 180

Query: 181 ESEFESRIRSRIMKFMNLSEDDYAMVFTANQSSAFKLLADTYPFQQNRNLITVYDHESEA 240
           ESE E  +R RIM +MN+SE DYAMVFTANQSSAFKLLAD+YPFQQN +L+TVYD++ EA
Sbjct: 181 ESEVEFEMRKRIMSYMNISECDYAMVFTANQSSAFKLLADSYPFQQNPSLLTVYDYKCEA 240

Query: 241 VDLMVESSRKKGARIYSAEFLWPNLNISTGKLRRLIVSKRKRKKKMKMKMNKRGLFVLPL 300
           VD+M ESS+KKG R+ SAEF WPN+ I + KLR+ I + +K +KK        GLFV PL
Sbjct: 241 VDVMTESSKKKGGRVMSAEFSWPNMRIQSRKLRKRIGNMKKTRKKP-------GLFVFPL 300

Query: 301 QSRLTGTPYSYQWLNIARDNEWDVCLDTCALGPKDMETLGLSLFKPEFLISSFYKVFGEN 360
           QSR+TG  YSY W++IA++N W V LD C+LGPKDM+TLGLSLF+P+FLI SF+KVFGEN
Sbjct: 301 QSRMTGARYSYMWMSIAQENGWHVLLDACSLGPKDMDTLGLSLFQPDFLICSFFKVFGEN 360

Query: 361 PSGFGCLFIKKSNVSLMESLLTSPANIGVITLISTSPSFPFTEEPETTETKTQQISKPTL 420
           PSGFGCLF+KKS+ S+++   T  ++IG+++L+  S    ++E+  + + +T +      
Sbjct: 361 PSGFGCLFVKKSSASVLKDS-TFASSIGIVSLVPASKPSEYSEDSISMDIETDKKQSKL- 420

Query: 421 EIQNLAIPESRNSPEITEATEIEEEELSITGIVESTTP--FVSTRSTNTEMNSYMDCRGL 480
                   E+  S EI E T I+++  S++ I++      F S++  + E+    +CRGL
Sbjct: 421 --------ENSKSHEIEEVT-IKQKAPSLSEIMKLDRDHHFESSQPKSAEI----ECRGL 480

Query: 481 DHADSVGLRLISIRARYLINWLTNALMNLQHPNPEGRIAKALVRIYGPKIEINRGPAVAF 540
           DHADS+GL LIS RARYLINWL NALM+LQHP+ +      LVRIYGPKI++ RGP++AF
Sbjct: 481 DHADSLGLVLISRRARYLINWLVNALMSLQHPHSQ--YGHRLVRIYGPKIKVERGPSLAF 540

Query: 541 NIFDWKGEKVDPAMVQKLADRSNISLSNGIVKEVSFLDKNEEENEMRKERAMEEGERIDR 600
           N+FDWKGEK+DP +VQKLADR+NISLSNGI+  + F DK+EEE E + E    +     R
Sbjct: 541 NVFDWKGEKIDPLIVQKLADRNNISLSNGILNHIWFSDKHEEERETKLETCASDRLVNKR 600

Query: 601 NEKRHCRIRVVSAGIGFLTNFEDVYKFWAFVSRFLDADFVEKERWRYMALNQK 641
            +  H  I VV+A +GFLTNFED+Y+ WAFVSRFLDADFVEKERWRYMALNQ+
Sbjct: 601 KDGCHSGISVVTAALGFLTNFEDIYRLWAFVSRFLDADFVEKERWRYMALNQR 629

BLAST of Cucsa.302680 vs. NCBI nr
Match: gi|1009166968|ref|XP_015901871.1| (PREDICTED: molybdenum cofactor sulfurase-like [Ziziphus jujuba])

HSP 1 Score: 669.5 bits (1726), Expect = 6.0e-189
Identity = 351/658 (53.34%), Postives = 469/658 (71.28%), Query Frame = 1

Query: 1   MQSPCIREASQACLRGCCRTPFLGLTDSSQTAIDRSSAASTPA---YNFHGTTETSLHPD 60
           MQSPCIREAS+A + GCC  PF+GL DS  +     + +   A   ++F   T ++L P+
Sbjct: 1   MQSPCIREASKALIHGCCAAPFVGLPDSHSSHSPDPTQSVWVAGSDHHFALATASTLPPN 60

Query: 61  ARFSDHESIPTLKDAFTYFIRAYPLYLDTQQIDRIRADEYNHLALSKHVCLDYNGQCLFS 120
           ++F++HES+P+L ++F+ FIRAYP Y +T   D+IR+ +Y HL +S HVCLDY G  LFS
Sbjct: 61  SQFTNHESLPSLLESFSKFIRAYPQYSETHLADQIRSQQYYHLTISNHVCLDYVGHGLFS 120

Query: 121 FAQQQ-----SSPMAPAASSSSPPGSPPLILHSPGSPFFNISHKAVKPNSQVKNGGQESE 180
           F+QQ      SS  + A+SSS+PP  PP++ +S    FF+IS+K+V   SQ+ +GG +SE
Sbjct: 121 FSQQHRKHFSSSTASFASSSSAPPPPPPIVQYSSEPNFFDISYKSVNLKSQLLHGGIDSE 180

Query: 181 FESRIRSRIMKFMNLSEDDYAMVFTANQSSAFKLLADTYPFQQNRNLITVYDHESEAVDL 240
            E ++RSRIM+FMN+SEDDY MVFTANQSSA KLLAD+YPFQ NRNL+TVYD+++EAV++
Sbjct: 181 IEMKVRSRIMEFMNVSEDDYTMVFTANQSSALKLLADSYPFQTNRNLLTVYDYKNEAVEV 240

Query: 241 MVESSRKKGARIYSAEFLWPNLNISTGKLRRLIVSKRKRKKKMKMKMNKRGLFVLPLQSR 300
           M+ESS+K+GAR+ +AE+ WPN+ I + KLR+++V KR  +        KRGLFV PLQSR
Sbjct: 241 MIESSKKRGARVMAAEYSWPNMRIQSRKLRKMVVHKRNTR--------KRGLFVFPLQSR 300

Query: 301 LTGTPYSYQWLNIARDNEWDVCLDTCALGPKDMETLGLSLFKPEFLISSFYKVFGENPSG 360
           +TG  Y Y W++IAR+N W V LD C+LGPKDMETLGL+LF+P+FLI SF+K+FGENPSG
Sbjct: 301 MTGARYPYLWMSIARENGWHVLLDACSLGPKDMETLGLALFRPDFLICSFFKIFGENPSG 360

Query: 361 FGCLFIKKSNVSLMESLLTSPANIGVITLISTSPSFP--FTEEPETTETKTQQISKPTLE 420
           FGCLF+KK+ VSL+    TS  +IG+++L+ TS   P  F +E  T + +T+Q  K   +
Sbjct: 361 FGCLFVKKTIVSLLTD-STSSTSIGIVSLLPTSTPLPSHFPQESTTEDKETEQ--KWNFD 420

Query: 421 IQNLAIPESRNSPEITEATEIEEEELSITGIVES-TTPFVSTRSTNTEM--NSYMDCRGL 480
              LA+          E   +E+ E S + +  S T PF S ++ N  +  NS ++ RGL
Sbjct: 421 SDGLAVSN-------IEEVSLEKRETSFSELGGSKTIPFESDQTKNKSVGGNSEIEFRGL 480

Query: 481 DHADSVGLRLISIRARYLINWLTNALMNLQHPNPEGRIAKALVRIYGPKIEINRGPAVAF 540
           DHADS+GL LIS R +YLINWL NAL++LQHP+ E      L+RIYGPKI  +RGP+VAF
Sbjct: 481 DHADSLGLILISSRVKYLINWLVNALISLQHPHAEN--GPPLIRIYGPKIRPDRGPSVAF 540

Query: 541 NIFDWKGEKVDPAMVQKLADRSNISLSNGIVKEVSFLDKNEEENEMRKERAMEEGERI-- 600
           N+FDWKGEK+DPA+VQKLADR+NISLS G ++ + F DKNEEE E + ER   E + I  
Sbjct: 541 NVFDWKGEKIDPALVQKLADRNNISLSYGFLQHIWFSDKNEEERETKIERFTAETKGIGL 600

Query: 601 ---DRNEKRHCRIRVVSAGIGFLTNFEDVYKFWAFVSRFLDADFVEKERWRYMALNQK 641
               R+E     I  V+A +G LTNFED+Y+ W FVSRFLDADFVEKERWRY+ALNQ+
Sbjct: 601 SNKSRDEYHSGGISAVTATLGMLTNFEDIYRLWVFVSRFLDADFVEKERWRYLALNQR 638