Cucsa.302540 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.302540 vs. Swiss-Prot
Match: C76B6_CATRO (Geraniol 8-hydroxylase OS=Catharanthus roseus GN=CYP76B6 PE=1 SV=1)

HSP 1 Score: 242.3 bits (617), Expect = 9.6e-63
Identity = 138/328 (42.07%), Postives = 199/328 (60.67%), Query Frame = 1

Query: 1   MDLLGIFIVLLSIAWLISMISK-TRPITNLPPGPKPLPLIGNLLALGDKPHQSLANLAKS 60
           MD L I + LL    L    S  +R   NLPPGP PLP IG+L  LGD+PH+SLA L+K 
Sbjct: 1   MDYLTIILTLLFALTLYEAFSYLSRRTKNLPPGPSPLPFIGSLHLLGDQPHKSLAKLSKK 60

Query: 61  YGPIMTLKLGQITTVVISSSAMAKQVLQTHDQFLSSRTVPDSMTTHNHDLFGLPWMPVSP 120
           +GPIM+LKLGQITT+VISSS MAK+VLQ  D   SSR+VP+++  HN   F + W+PV+ 
Sbjct: 61  HGPIMSLKLGQITTIVISSSTMAKEVLQKQDLAFSSRSVPNALHAHNQFKFSVVWLPVAS 120

Query: 121 LWRNLRRICNTQLFAARILDANENLRRGQVAELVTEISRCALKGNETVDFGKVAFVTSVN 180
            WR+LR++ N+ +F+   LDAN++LR  +V EL+    + +  G E VD G+ AF TS+N
Sbjct: 121 RWRSLRKVLNSNIFSGNRLDANQHLRTRKVQELIAYCRKNSQSG-EAVDVGRAAFRTSLN 180

Query: 181 LLWNTIFSEDFVDPNSKIGREFKVAVRGAMEEAAKPNFGDYFALLKRFDVQGIKKKQSVH 240
           LL N IFS+D  DP S   +EFK  V   M EA KPN  D+F LL++ D QGI+ + ++H
Sbjct: 181 LLSNLIFSKDLTDPYSDSAKEFKDLVWNIMVEAGKPNLVDFFPLLEKVDPQGIRHRMTIH 240

Query: 241 FDRIFDVLEQMIDERIDEQKKKSDISR-LPYLQAVIKETLRCHSPPFLLPRKALQDVEIS 300
           F  +  +   +++ER+++++ K + +  L  L    +E+      P  + R  ++ + + 
Sbjct: 241 FGEVLKLFGGLVNERLEQRRSKGEKNDVLDVLLTTSQES------PEEIDRTHIERMCLD 300

Query: 301 GFTIPKDAQVPVNLWAMGRDSNVWKNPE 327
            F    D       WAM   S + KNP+
Sbjct: 301 LFVAGTDTTSSTLEWAM---SEMLKNPD 318

HSP 2 Score: 206.5 bits (524), Expect = 5.8e-52
Identity = 96/153 (62.75%), Postives = 122/153 (79.74%), Query Frame = 1

Query: 260 KKSDISRLPYLQAVIKETLRCHSP-PFLLPRKALQDVEISGFTIPKDAQVPVNLWAMGRD 319
           ++SDI+RLPYL+ V+KETLR H P PFL+PRK  Q VE+ G+ +PK +QV VN WA+GRD
Sbjct: 338 EESDINRLPYLRCVMKETLRIHPPVPFLIPRKVEQSVEVCGYNVPKGSQVLVNAWAIGRD 397

Query: 320 SNVWKNPEIFEPERFLEMEIDIKGRDFELVPFGGGRRICPELSLAMRMLPLMLGSLLHFF 379
             VW +   F+PERF+E E+DI+GRDFEL+PFG GRRICP L LA+R +PLMLGSLL+ F
Sbjct: 398 ETVWDDALAFKPERFMESELDIRGRDFELIPFGAGRRICPGLPLALRTVPLMLGSLLNSF 457

Query: 380 DWKLEDGFRPDDLNMDEKYGLTLEMASPLRAFP 412
           +WKLE G  P DL+M+EK+G+TL+ A PLRA P
Sbjct: 458 NWKLEGGMAPKDLDMEEKFGITLQKAHPLRAVP 490

BLAST of Cucsa.302540 vs. Swiss-Prot
Match: C76BA_SWEMU (Geraniol 8-hydroxylase OS=Swertia mussotii GN=CYP76B10 PE=1 SV=1)

HSP 1 Score: 241.1 bits (614), Expect = 2.1e-62
Identity = 135/325 (41.54%), Postives = 197/325 (60.62%), Query Frame = 1

Query: 23  TRPITNLPPGPKPLPLIGNLLALGDKPHQSLANLAKSYGPIMTLKLGQITTVVISSSAMA 82
           +R   NLPPGP PLPLIGNL  LGD+PH+SLA LAK +GPIM L+LGQ+TT+V++SS MA
Sbjct: 26  SRKSKNLPPGPSPLPLIGNLHLLGDQPHKSLAKLAKKHGPIMGLQLGQVTTIVVTSSGMA 85

Query: 83  KQVLQTHDQFLSSRTVPDSMTTHNHDLFGLPWMPVSPLWRNLRRICNTQLFAARILDANE 142
           K+VLQ  D   SSR++P+++  H+   + + W+PV+  WR LR+  N+ +F+   LDAN+
Sbjct: 86  KEVLQKQDLAFSSRSIPNAIHAHDQYKYSVIWLPVASRWRGLRKALNSNMFSGNRLDANQ 145

Query: 143 NLRRGQVAELVTEISRCALKGNETVDFGKVAFVTSVNLLWNTIFSEDFVDPNSKIGREFK 202
           +LR  +V EL+    + +  G + +D G+ AF TS+NLL NT+FS+D  DP S   +EFK
Sbjct: 146 HLRSRKVQELIAYCRKSSQTG-DAIDVGRAAFRTSLNLLSNTMFSKDLTDPYSDSAKEFK 205

Query: 203 VAVRGAMEEAAKPNFGDYFALLKRFDVQGIKKKQSVHFDRIFDVLEQMIDERIDEQKKKS 262
             V   M EA KPN  DYF LL + D QGI+K+ ++HF +I ++   +IDER+ ++K K 
Sbjct: 206 DLVWNVMVEAGKPNLVDYFPLLDKVDPQGIRKRMTIHFGKILELFGGLIDERLQQKKAKG 265

Query: 263 -DISRLPYLQAVIKETLRCHSPPFLLPRKALQDVEISGFTIPKDAQVPVNLWAMGRDSNV 322
            +   L  L    +E+      P  + R  +Q + +  F    D       WAM   S +
Sbjct: 266 VNDDVLDVLLTTSEES------PEEIDRTHIQRMCLDLFVAGTDTTSSTLEWAM---SEM 325

Query: 323 WKNPEIFEPERFLEMEIDIKGRDFE 347
            KNPE  +  +    ++  KG+  E
Sbjct: 326 LKNPEKMKAAQAELAQVIGKGKAVE 340

HSP 2 Score: 203.8 bits (517), Expect = 3.8e-51
Identity = 91/151 (60.26%), Postives = 125/151 (82.78%), Query Frame = 1

Query: 260 KKSDISRLPYLQAVIKETLRCHSP-PFLLPRKALQDVEISGFTIPKDAQVPVNLWAMGRD 319
           +++D++RLPYL+  IKETLR H P P L+PR+  Q+VE+ G+T+PK++QV VN+WA+ RD
Sbjct: 340 EEADLARLPYLRCAIKETLRIHPPVPLLIPRRTEQEVEVCGYTVPKNSQVLVNVWAISRD 399

Query: 320 SNVWKNPEIFEPERFLEMEIDIKGRDFELVPFGGGRRICPELSLAMRMLPLMLGSLLHFF 379
             +WK+P  F+PERFLE E++++G+DFEL+PFG GRRICP L LA+RM+P+MLGSLL+ F
Sbjct: 400 DAIWKDPLSFKPERFLESELEMRGKDFELIPFGAGRRICPGLPLAVRMVPVMLGSLLNSF 459

Query: 380 DWKLEDGFRPDDLNMDEKYGLTLEMASPLRA 410
           DWKLE G  P DL+M+EK+G+TL+ A PLRA
Sbjct: 460 DWKLEGGIAPKDLDMEEKFGITLQKAHPLRA 490

BLAST of Cucsa.302540 vs. Swiss-Prot
Match: C76B1_HELTU (7-ethoxycoumarin O-deethylase OS=Helianthus tuberosus GN=CYP76B1 PE=1 SV=1)

HSP 1 Score: 214.9 bits (546), Expect = 1.6e-54
Identity = 116/255 (45.49%), Postives = 165/255 (64.71%), Query Frame = 1

Query: 1   MDLLGIFIVLLSIAWLISMISKTRPITNLPPGPKPLPLIGNLLALGDKPHQSLANLAKSY 60
           MD L I   LL    LI ++   +P  NLPPGP  LP+IGNL  LG  PHQSLA LAK +
Sbjct: 1   MDFLIIVSTLLLSYILIWVLGVGKP-KNLPPGPTRLPIIGNLHLLGALPHQSLAKLAKIH 60

Query: 61  GPIMTLKLGQITTVVISSSAMAKQVLQTHDQFLSSRTVPDSMTTHNHDLFGLPWMPVSPL 120
           GPIM+L+LGQITT+VISS+  A++VL+  D   S+R VPD++  +NH+   + ++ V   
Sbjct: 61  GPIMSLQLGQITTLVISSATAAEEVLKKQDLAFSTRNVPDAVRAYNHERHSISFLHVCTE 120

Query: 121 WRNLRRICNTQLFAARILDANENLRRGQVAELVTEISRCALKGNETVDFGKVAFVTSVNL 180
           WR LRRI ++ +F+   L+A ++LR  +V EL+    + AL  NE V  G+ AF TS+NL
Sbjct: 121 WRTLRRIVSSNIFSNSSLEAKQHLRSKKVEELIAYCRKAAL-SNENVHIGRAAFRTSLNL 180

Query: 181 LWNTIFSEDFVDPNSKIGREFKVAVRGAMEEAAKPNFGDYFALLKRFDVQGIKKKQSVHF 240
           L NTIFS+D  DP    G+EF+  +   M ++AK N  D F +LK+ D QGIK+  + HF
Sbjct: 181 LSNTIFSKDLTDPYEDSGKEFREVITNIMVDSAKTNLVDVFPVLKKIDPQGIKRGMARHF 240

Query: 241 DRIFDVLEQMIDERI 256
            ++  + +Q+I+ER+
Sbjct: 241 SKVLGIFDQLIEERM 253

HSP 2 Score: 173.3 bits (438), Expect = 5.5e-42
Identity = 82/154 (53.25%), Postives = 107/154 (69.48%), Query Frame = 1

Query: 260 KKSDISRLPYLQAVIKETLRCHSP-PFLLPRKALQDVEISGFTIPKDAQVPVNLWAMGRD 319
           K+ D+ RLPYL  ++KE LR H P P LLPRK +  VE+SG+TIP    V VN WA+GRD
Sbjct: 334 KEDDVLRLPYLSCIVKEVLRLHPPSPLLLPRKVVTQVELSGYTIPAGTLVFVNAWAIGRD 393

Query: 320 SNVWKNPEIFEPERFLEMEIDIKGRDFELVPFGGGRRICPELSLAMRMLPLMLGSLLHFF 379
             VW +   F+P+RFLE  +D++G DF+L+PFG GRRICP + LA RM+P+MLGSLL+ F
Sbjct: 394 PTVWDDSLEFKPQRFLESRLDVRGHDFDLIPFGAGRRICPGIPLATRMVPIMLGSLLNNF 453

Query: 380 DWKLEDGFRPDDLNMDEKYGLTLEMASPLRAFPL 413
           DWK++     D L+M EK G T+  A PL   P+
Sbjct: 454 DWKIDTKVPYDVLDMTEKNGTTISKAKPLCVVPI 487

BLAST of Cucsa.302540 vs. Swiss-Prot
Match: C76AD_BETVU (Cytochrome P450 76AD1 OS=Beta vulgaris GN=CYP76AD1 PE=2 SV=1)

HSP 1 Score: 208.8 bits (530), Expect = 1.2e-52
Identity = 98/158 (62.03%), Postives = 123/158 (77.85%), Query Frame = 1

Query: 256 DEQKKKSDISRLPYLQAVIKETLRCHSPP-FLLPRKALQDVEISGFTIPKDAQVPVNLWA 315
           D+Q ++SDI  LPYLQA+IKETLR H P  FLLPRKA  DVE+ G+ +PKDAQ+ VNLWA
Sbjct: 337 DKQIQESDIINLPYLQAIIKETLRLHPPTVFLLPRKADTDVELYGYIVPKDAQILVNLWA 396

Query: 316 MGRDSNVWKNPEIFEPERFLEMEIDIKGRDFELVPFGGGRRICPELSLAMRMLPLMLGSL 375
           +GRD N W+N +IF PERF+  EID+KGRDF L+PFG GRRICP ++LA+RML LML +L
Sbjct: 397 IGRDPNAWQNADIFSPERFIGCEIDVKGRDFGLLPFGAGRRICPGMNLAIRMLTLMLATL 456

Query: 376 LHFFDWKLEDGFRPDDLNMDEKYGLTLEMASPLRAFPL 413
           L FF+WKLE    P DL+MDEK+G+ L+   PL+  P+
Sbjct: 457 LQFFNWKLEGDISPKDLDMDEKFGIALQKTKPLKLIPI 494

HSP 2 Score: 206.8 bits (525), Expect = 4.4e-52
Identity = 103/253 (40.71%), Postives = 156/253 (61.66%), Query Frame = 1

Query: 8   IVLLSIAWLIS-----MISKTRPITNLPPGPKPLPLIGNLLALGDKPHQSLANLAKSYGP 67
           + ++   W IS     ++   +    LPPGPKPLP+IGN+L +G KPH+S ANLAK +GP
Sbjct: 6   LAMILAIWFISFHFIKLLFSQQTTKLLPPGPKPLPIIGNILEVGKKPHRSFANLAKIHGP 65

Query: 68  IMTLKLGQITTVVISSSAMAKQVLQTHDQFLSSRTVPDSMTTHNHDLFGLPWMPVSPLWR 127
           +++L+LG +TT+V+SS+ +AK++    D  LS+RT+P+S+T  +H    + W+PVSP WR
Sbjct: 66  LISLRLGSVTTIVVSSADVAKEMFLKKDHPLSNRTIPNSVTAGDHHKLTMSWLPVSPKWR 125

Query: 128 NLRRICNTQLFAARILDANENLRRGQVAELVTEISRCALKGNETVDFGKVAFVTSVNLLW 187
           N R+I    L + + LDA +  R  +V +L   +  CA KG + VD GK AF TS+NLL 
Sbjct: 126 NFRKITAVHLLSPQRLDACQTFRHAKVQQLYEYVQECAQKG-QAVDIGKAAFTTSLNLLS 185

Query: 188 NTIFSEDFVDPNSKIGREFKVAVRGAMEEAAKPNFGDYFALLKRFDVQGIKKKQSVHFDR 247
              FS +     S   +EFK  +   ME+  KPN+ DYF +L   D  GI+++ +  FD+
Sbjct: 186 KLFFSVELAHHKSHTSQEFKELIWNIMEDIGKPNYADYFPILGCVDPSGIRRRLACSFDK 245

Query: 248 IFDVLEQMIDERI 256
           +  V + +I ER+
Sbjct: 246 LIAVFQGIICERL 257

BLAST of Cucsa.302540 vs. Swiss-Prot
Match: C76AN_BETVU (Inactive cytochrome P450 76AD1 OS=Beta vulgaris GN=CYP76AD1 PE=3 SV=1)

HSP 1 Score: 207.6 bits (527), Expect = 2.6e-52
Identity = 103/253 (40.71%), Postives = 156/253 (61.66%), Query Frame = 1

Query: 8   IVLLSIAWLIS-----MISKTRPITNLPPGPKPLPLIGNLLALGDKPHQSLANLAKSYGP 67
           + ++   W IS     ++   +    LPPGPKPLP+IGN+L +G KPH+S ANLAK +GP
Sbjct: 6   LAMILAIWFISFHFIKLLFSQQTTKLLPPGPKPLPIIGNILEVGKKPHRSFANLAKIHGP 65

Query: 68  IMTLKLGQITTVVISSSAMAKQVLQTHDQFLSSRTVPDSMTTHNHDLFGLPWMPVSPLWR 127
           +++L+LG +TT+V+SS+ +AK++    D  LS+RT+P+S+T  +H    + W+PVSP WR
Sbjct: 66  LISLRLGSVTTIVVSSADVAKEMFLKKDHPLSNRTIPNSVTAGDHHKLTMSWLPVSPKWR 125

Query: 128 NLRRICNTQLFAARILDANENLRRGQVAELVTEISRCALKGNETVDFGKVAFVTSVNLLW 187
           N R+I    L + + LDA +  R  +V +L   +  CA KG + VD GK AF TS+NLL 
Sbjct: 126 NFRKITAVHLLSPQRLDACQTFRHAKVQQLYEYVQECAQKG-QAVDIGKAAFTTSLNLLS 185

Query: 188 NTIFSEDFVDPNSKIGREFKVAVRGAMEEAAKPNFGDYFALLKRFDVQGIKKKQSVHFDR 247
              FS +     S   +EFK  +   ME+  KPN+ DYF +L   D  GI+++ +  FD+
Sbjct: 186 KLFFSVELAHHKSHTSQEFKELIWNIMEDIGKPNYADYFPILGCVDPSGIRRRLACSFDK 245

Query: 248 IFDVLEQMIDERI 256
           +  V + +I ER+
Sbjct: 246 LIAVFQSIICERL 257

HSP 2 Score: 70.5 bits (171), Expect = 5.0e-11
Identity = 35/54 (64.81%), Postives = 43/54 (79.63%), Query Frame = 1

Query: 256 DEQKKKSDISRLPYLQAVIKETLRCHSPP-FLLPRKALQDVEISGFTIPKDAQV 309
           D+Q ++SDI  LPYLQA+IKETLR H P  FLLPRKA  DVE+ G+ +PKDAQ+
Sbjct: 337 DKQIQESDIINLPYLQAIIKETLRLHPPTVFLLPRKADTDVELYGYIVPKDAQI 390

BLAST of Cucsa.302540 vs. TrEMBL
Match: A0A0A0KP24_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G157260 PE=4 SV=1)

HSP 1 Score: 484.6 bits (1246), Expect = 1.2e-133
Identity = 241/251 (96.02%), Postives = 246/251 (98.01%), Query Frame = 1

Query: 19  MISKTRPITNLPPGPKPLPLIGNLLALGDKPHQSLANLAKSYGPIMTLKLGQITTVVISS 78
           MISKTRPITNLPPGPKPLPLIGNLLALGDKPHQSLANLAKSYGPIMTLKLGQITTVVISS
Sbjct: 1   MISKTRPITNLPPGPKPLPLIGNLLALGDKPHQSLANLAKSYGPIMTLKLGQITTVVISS 60

Query: 79  SAMAKQVLQTHDQFLSSRTVPDSMTTHNHDLFGLPWMPVSPLWRNLRRICNTQLFAARIL 138
           SAMAKQVLQTHDQFLSSRTVPDSMTTHNHDLFGLPWMPVSPLWRNLRRICNTQLFAARIL
Sbjct: 61  SAMAKQVLQTHDQFLSSRTVPDSMTTHNHDLFGLPWMPVSPLWRNLRRICNTQLFAARIL 120

Query: 139 DANENLRRGQVAELVTEISRCALKGNETVDFGKVAFVTSVNLLWNTIFSEDFVDPNSKIG 198
           DANENLRRGQVAELVTEISRCALKGNETVDFGKVAFVTS+NLLWNTIFSEDFVDPNSKIG
Sbjct: 121 DANENLRRGQVAELVTEISRCALKGNETVDFGKVAFVTSMNLLWNTIFSEDFVDPNSKIG 180

Query: 199 REFKVAVRGAMEEAAKPNFGDYFALLKRFDVQGIKKKQSVHFDRIFDVLEQMIDERIDEQ 258
           REFKVAVRGAMEEAAKPNFGDYFALLKRFDVQGIKKKQSVHFDRIFDVLEQMIDERIDEQ
Sbjct: 181 REFKVAVRGAMEEAAKPNFGDYFALLKRFDVQGIKKKQSVHFDRIFDVLEQMIDERIDEQ 240

Query: 259 KKKSDISRLPY 270
           KK    +++ +
Sbjct: 241 KKSCGSNKIKH 251

BLAST of Cucsa.302540 vs. TrEMBL
Match: A0A0B0PL49_GOSAR (7-ethoxycoumarin O-deethylase OS=Gossypium arboreum GN=F383_04436 PE=3 SV=1)

HSP 1 Score: 420.6 bits (1080), Expect = 2.2e-114
Identity = 214/438 (48.86%), Postives = 289/438 (65.98%), Query Frame = 1

Query: 1   MDLLGIFIVLLSIAWLISMI------SKTRPITNLPPGPKPLPLIGNLLALGDKPHQSLA 60
           MDLL   ++ L   W +          +      LPPGP P P++GNL  LGDKPH+SLA
Sbjct: 1   MDLLTSSLLCLLFTWFLLQAFNSFRNGRKSSQRKLPPGPWPKPIVGNLFDLGDKPHRSLA 60

Query: 61  NLAKSYGPIMTLKLGQITTVVISSSAMAKQVLQTHDQFLSSRTVPDSMTTHNHDLFGLPW 120
            LA+ +GP+M+LKLG I T+V+SS   AK++LQ  D    +RT+ D++    H   GLPW
Sbjct: 61  KLAQIHGPVMSLKLGSILTIVVSSETTAKEILQKQDLTFCNRTIVDAVRASQHYEVGLPW 120

Query: 121 MPVSPLWRNLRRICNTQLFAARILDANENLRRGQVAELVTEISRCALKGNETVDFGKVAF 180
           +PVSPLWR LR++C T +FA+  LDANE LR+ ++ EL+  +    LKG E V+ G+VAF
Sbjct: 121 IPVSPLWRTLRKVCKTHIFASIKLDANEYLRQNKIQELIANVGESCLKG-EAVNIGQVAF 180

Query: 181 VTSVNLLWNTIFSEDFVDPNSKIGREFKVAVRGAMEEAAKPNFGDYFALLKRFDVQGI-- 240
            T++NLL NT+FS D VDPNS I REFK  VRG M+E   PN  D+F  L++ D QG+  
Sbjct: 181 DTTINLLSNTVFSMDLVDPNSSIAREFKKTVRGMMDEVGTPNLADFFPFLRKIDPQGVLF 240

Query: 241 -------------KKKQSVHFDRIFDVLEQMIDERIDEQK--KKSDISRLPYLQAVIKET 300
                           + +   ++    ++ +++ I++ K  ++SDI+ LPYLQA+IKET
Sbjct: 241 VAGTDTTSSTLEWAMAELLQNPQVLLKAKKELNQAIEKGKPIEESDINHLPYLQAIIKET 300

Query: 301 LRCHSP-PFLLPRKALQDVEISGFTIPKDAQVPVNLWAMGRDSNVWKNPEIFEPERFLEM 360
            R H   P LLPR+A  D E+ GF +PK +QV VN+WA+GRD ++WKNP  F PERFL  
Sbjct: 301 FRMHPVVPLLLPRRAGSDAELCGFKVPKGSQVLVNVWAIGRDPSIWKNPNSFMPERFLGS 360

Query: 361 EIDIKGRDFELVPFGGGRRICPELSLAMRMLPLMLGSLLHFFDWKLEDGFRPDDLNMDEK 415
           EID+KGRDF L+PFG GRRICP L LA RML LMLGSL++ FDWKLE G  P+++NM+EK
Sbjct: 361 EIDVKGRDFGLIPFGAGRRICPGLPLANRMLHLMLGSLINSFDWKLEGGISPNEINMEEK 420

BLAST of Cucsa.302540 vs. TrEMBL
Match: A0A067DPV6_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g043641mg PE=3 SV=1)

HSP 1 Score: 391.0 bits (1003), Expect = 1.9e-105
Identity = 214/469 (45.63%), Postives = 294/469 (62.69%), Query Frame = 1

Query: 9   VLLSIAWLISMISKTRPITNLPPGPKPLPLIGNLLALGDKPHQSLANLAKSYGPIMTLKL 68
           +L +  W+I++ S ++    LPPGP PLP+IGNLL LG KPH+SLA+LAK +GPIM+L+ 
Sbjct: 11  LLFTWVWVITLRSFSKGSRKLPPGPTPLPIIGNLLELGQKPHKSLADLAKVHGPIMSLQF 70

Query: 69  GQITTVVISSSAMAKQVLQTHDQFLSSRTVPDSMTTHNHDLFGLPWMPVSPLWRNLRRIC 128
           GQ+TTVVISS+AMAKQVLQ HD  L +R VP+S+ +   D +G+PW+PVS  W+ LR+IC
Sbjct: 71  GQVTTVVISSAAMAKQVLQDHDTSLCNRNVPESIRSIQKDEYGIPWLPVSTKWKKLRKIC 130

Query: 129 NTQLFAARILDANENLRRGQVAELVTEISRCALKGNETVDFGKVAFVTSVNLLWNTIFSE 188
           N  +F ++ LDAN++LR  ++ +LV  +      G E  D G+ AF   VN L NTIFS 
Sbjct: 131 NLHIFTSQKLDANQDLRPKKIQQLVAFVQDSCRTG-EPSDVGQAAFDIVVNFLSNTIFSI 190

Query: 189 DFVDPNSKIGREFKVAVRGAMEEAAKPNFGDYFALLKRFDVQGIKK-------------- 248
           D  DPNS   REFK ++ G M EA +PN  D+F +L++ D+QG+++              
Sbjct: 191 DLADPNSDSAREFKDSMWGLMVEAKRPNLSDFFPMLRKLDIQGVQRRITRHTIKILEVLD 250

Query: 249 ---------KQSVHFDRIFDVLEQMID----ERID------------------------- 308
                    +Q   F    D+L+ +++    E ID                         
Sbjct: 251 RFIDQRLEQRQQNSFADSKDMLDTLLNISESEDIDRNDIKFVILAVTELLNNPEALSKAR 310

Query: 309 ---EQK-------KKSDISRLPYLQAVIKETLRCHSP-PFLLPRKALQDVEISGFTIPKD 368
              EQK       ++SDI++LPYLQ ++KETLR H P P LLPRKA  DVEI+GF IPK 
Sbjct: 311 LELEQKVGKGNLIEESDITQLPYLQIIVKETLRLHPPVPLLLPRKASADVEITGFIIPKG 370

Query: 369 AQVPVNLWAMGRDSNVWKNPEIFEPERFLEMEIDIKGRDFELVPFGGGRRICPELSLAMR 415
           AQV VN WA+GRD++ W +P  F+ ERFL  ++D+KGR+FEL PFG GRRIC  L LA+R
Sbjct: 371 AQVLVNAWAIGRDTSTWDDPYSFKLERFLGSDVDVKGRNFELFPFGAGRRICLGLPLAIR 430

BLAST of Cucsa.302540 vs. TrEMBL
Match: A0A166B056_DAUCA (Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_010932 PE=4 SV=1)

HSP 1 Score: 362.1 bits (928), Expect = 9.2e-97
Identity = 192/413 (46.49%), Postives = 275/413 (66.59%), Query Frame = 1

Query: 3   LLGIFIVLLSIAWLISMISKTRPITNLPPGPKPLPLIGNLLALGDKPHQSLANLAKSYGP 62
           +L +  ++L+ A+  S+   +R    LPPGP  LP+IGNL  LGD PHQSLA LA  YGP
Sbjct: 4   VLTVIYMILAFAFFKSLFLFSRRGKKLPPGPFQLPIIGNLATLGDLPHQSLAKLADIYGP 63

Query: 63  IMTLKLGQITTVVISSSAMAKQVLQTHDQFLSSRTVPDSMTTHNHDLFGLPWMPVSPLWR 122
           IM LK+G + T+VISS A+A+QVLQ  D   S R +PD++   +H  + + ++PVSPLW 
Sbjct: 64  IMHLKMGCLDTIVISSPAIAQQVLQKQDIIFSRRFIPDAIFACDHFKYSVAFLPVSPLWS 123

Query: 123 NLRRICNTQLFAARILDANENLRRGQVAELVTEISRCALKGNETVDFGKVAFVTSVNLLW 182
           NLR+I ++ LF+   LDAN++LR  +V +L+  + +C+  G E +D G+ AF TS+NLL 
Sbjct: 124 NLRKIIHSNLFSFNKLDANQHLRSSKVNDLIGYVKKCSQTG-EAIDIGRAAFTTSLNLLS 183

Query: 183 NTIFSEDFVDPNSKIGREFK---VAVRGAMEEAAKPNFGDYFALLKRFDVQGIKKKQSVH 242
           NTIFS+D  DP+    +EFK   + V G    ++   +G   A L R     +K K    
Sbjct: 184 NTIFSKDMADPSQDSSKEFKDLDIFVAGTDTTSSTVEWG--MAELLRNSETMVKAKAE-- 243

Query: 243 FDRIFDVLEQMIDERIDEQK--KKSDISRLPYLQAVIKETLRCHSP-PFLLPRKALQDVE 302
                      +D+ + + K  +++DI+RLPYLQ +++ETLR H P PFLLPR+  ++ E
Sbjct: 244 -----------LDKVLGKGKILEEADITRLPYLQCIVRETLRLHPPIPFLLPRQIAEEAE 303

Query: 303 ISGFTIPKDAQVPVNLWAMGRDSNVWKNPEIFEPERFLEMEIDIKGRDFELVPFGGGRRI 362
           ++G+TIPK++QV VN+WA+GRD + WKNP  F PERFL+ +ID+KG+DFEL+PFG GRRI
Sbjct: 304 VNGYTIPKNSQVLVNVWAIGRDQSSWKNPLSFYPERFLDSDIDLKGQDFELIPFGAGRRI 363

Query: 363 CPELSLAMRMLPLMLGSLLHFFDWKLEDGFRPDDLNMDEKYGLTLEMASPLRA 410
           CP +SLA R +P++LGSL++ FDW+LE     ++L MDEK+G+TL    PL A
Sbjct: 364 CPGMSLASRTVPVVLGSLINCFDWELEGKISANELGMDEKFGITLGKLHPLCA 400

BLAST of Cucsa.302540 vs. TrEMBL
Match: A0A103XBR0_CYNCS (Cytochrome P450 OS=Cynara cardunculus var. scolymus GN=Ccrd_024910 PE=3 SV=1)

HSP 1 Score: 355.5 bits (911), Expect = 8.6e-95
Identity = 196/424 (46.23%), Postives = 265/424 (62.50%), Query Frame = 1

Query: 6   IFIVLLSIA----WLISMISKTRP---ITNLPPGPKPLPLIGNLLALGDKPHQSLANLAK 65
           I  +LLSI+    + ++ +S+ R    IT LPPGP P P+IGN+L LG+KPH SLA L+K
Sbjct: 5   IIFILLSISTCFFYFLNSLSRRRKSGVITRLPPGPYPFPIIGNILKLGNKPHHSLAALSK 64

Query: 66  SYGPIMTLKLGQITTVVISSSAMAKQVLQTHDQFLSSRTVPDSMTTHNHDLFGLPWMPVS 125
           +YGP+M+LKLG  TT+V+SS  +A++    HD   SSR++P  + TH+HD   + W+PV 
Sbjct: 65  TYGPLMSLKLGTTTTIVVSSREIAQEFFLKHDVSFSSRSIPHVVNTHDHDKLSMAWLPVG 124

Query: 126 PLWRNLRRICNTQLFAARILDANENLRRGQVAELVTEISRCALKGNETVDFGKVAFVTSV 185
             WR LRRI    LF  R LDA+  LR+ +V +LV  +      G + V+ G+ A  T++
Sbjct: 125 DQWRKLRRISKEHLFVVRQLDASHLLRKEKVQQLVDHVQGYCDNG-KPVNIGQTAATTTL 184

Query: 186 NLLWNTIFSEDFVDPNSKIGREFKVAVRGAMEEAAKPNFGDYFALLKRFDVQGIKKKQSV 245
           N+L N IFS D    +S+  ++FK  V   ME   KPN  D+F  L     +        
Sbjct: 185 NVLSNFIFSIDLAQYDSRSSQDFKDLVWSLMEVGGKPNIADFFPDLFIAGTETTSSTLEW 244

Query: 246 HFDRIFDVLEQM------IDERI---DEQKKKSDISRLPYLQAVIKETLRCHSP-PFLLP 305
               +    EQM      ++E I   D   ++SDISRLPYL A++KETLR H P  FLLP
Sbjct: 245 AMAELIHNPEQMSKARSELEEVIGKEDRTFQESDISRLPYLHAIVKETLRLHPPVTFLLP 304

Query: 306 RKALQDVEISGFTIPKDAQVPVNLWAMGRDSNVWKNPEIFEPERFLEMEIDIKGRDFELV 365
            KA +DVEI G+ IPKD Q+  NLW MG+DSN W + + F+P+RFL++ ID KGRD EL+
Sbjct: 305 HKATKDVEIQGYIIPKDTQILCNLWGMGQDSNTWSDAQRFKPQRFLDVGIDYKGRDLELI 364

Query: 366 PFGGGRRICPELSLAMRMLPLMLGSLLHFFDWKLEDGFRPDDLNMDEKYGLTLEMASPLR 413
           PFG GRRICP L LA RML LMLGSL++ FDWK+E+G   D   M +K+G+TL+   PL 
Sbjct: 365 PFGTGRRICPGLPLAHRMLHLMLGSLIYKFDWKIEEGMVMD---MSDKFGMTLQKNLPLM 424

BLAST of Cucsa.302540 vs. TAIR10
Match: AT2G45570.1 (AT2G45570.1 cytochrome P450, family 76, subfamily C, polypeptide 2)

HSP 1 Score: 198.4 bits (503), Expect = 8.9e-51
Identity = 94/154 (61.04%), Postives = 119/154 (77.27%), Query Frame = 1

Query: 260 KKSDISRLPYLQAVIKETLRCH-SPPFLLPRKALQDVEISGFTIPKDAQVPVNLWAMGRD 319
           ++SDIS LPYLQAV+KET R H + P L+PRKA  DVE+ GF +PKD QV VN+WA+GRD
Sbjct: 353 EESDISALPYLQAVVKETFRLHPAAPLLVPRKAESDVEVLGFMVPKDTQVFVNVWAIGRD 412

Query: 320 SNVWKNPEIFEPERFLEMEIDIKGRDFELVPFGGGRRICPELSLAMRMLPLMLGSLLHFF 379
            NVW+N   F+PERFL  +ID++GRD+EL PFG GRRICP L LA++ +PLML SLL+ F
Sbjct: 413 PNVWENSSRFKPERFLGKDIDLRGRDYELTPFGAGRRICPGLPLAVKTVPLMLASLLYSF 472

Query: 380 DWKLEDGFRPDDLNMDEKYGLTLEMASPLRAFPL 413
           DWKL +G   +DL+MDE +GLTL   +PL A P+
Sbjct: 473 DWKLPNGVGSEDLDMDETFGLTLHKTNPLHAVPV 506

HSP 2 Score: 176.4 bits (446), Expect = 3.6e-44
Identity = 100/270 (37.04%), Postives = 158/270 (58.52%), Query Frame = 1

Query: 3   LLGIFIVLLSIAWLISMISKTRP-----ITNLPPGPKPLPLIGNLLALGDKPHQSLANLA 62
           L  +F  +LS  + I   + TRP     +   PPGP  LP+IGN+  +G  PH S A+L+
Sbjct: 9   LFPLFCFVLS--FFIIFFTTTRPRSSRKVVPSPPGPPRLPIIGNIHLVGRNPHHSFADLS 68

Query: 63  KSYGPIMTLKLGQITTVVISSSAMAKQVLQTHDQFLSSRTVPDSMTTHNHDLFGLPWM-P 122
           K+YGPIM+LK G + TVV++S   A++VL+T+DQ LSSRT  +S+ + NHD   + W+ P
Sbjct: 69  KTYGPIMSLKFGSLNTVVVTSPEAAREVLRTYDQILSSRTPTNSIRSINHDKVSVVWLPP 128

Query: 123 VSPLWRNLRRICNTQLFAARILDANENLRRGQVAELVTEISRCALKGNETVDFGKVAFVT 182
            S  WR LR++  TQLF+ + ++A + LR  +V ELV+ +S  + +  E VD  +  F+T
Sbjct: 129 SSSRWRLLRKLSATQLFSPQRIEATKTLRENKVKELVSFMSESS-EREEAVDISRATFIT 188

Query: 183 SVNLLWNTIFSEDFVDPNSKIGREFKVAVRGAMEEAAKPNFGDYFALLKRFDVQGIKKKQ 242
           ++N++ N +FS D  + +S     F+  V G ME    P+  ++F  L   D+QG +K  
Sbjct: 189 ALNIISNILFSVDLGNYDSNKSGVFQDTVIGVMEAVGNPDAANFFPFLGFLDLQGNRKTL 248

Query: 243 SVHFDRIFDVLEQMIDERIDEQKKKSDISR 267
               +R+F V    ID ++ E+  +   S+
Sbjct: 249 KACSERLFKVFRGFIDAKLAEKSLRDTNSK 275

BLAST of Cucsa.302540 vs. TAIR10
Match: AT2G45550.1 (AT2G45550.1 cytochrome P450, family 76, subfamily C, polypeptide 4)

HSP 1 Score: 196.4 bits (498), Expect = 3.4e-50
Identity = 93/154 (60.39%), Postives = 119/154 (77.27%), Query Frame = 1

Query: 260 KKSDISRLPYLQAVIKETLRCH-SPPFLLPRKALQDVEISGFTIPKDAQVPVNLWAMGRD 319
           ++SDIS LPYLQAV+KET R H + P L+PRKA  DVE+ GF +PKD QV VN+WA+GRD
Sbjct: 352 QESDISGLPYLQAVVKETFRLHPAAPLLVPRKAESDVEVLGFMVPKDTQVLVNVWAIGRD 411

Query: 320 SNVWKNPEIFEPERFLEMEIDIKGRDFELVPFGGGRRICPELSLAMRMLPLMLGSLLHFF 379
            +VW+NP  FEPERF+  +ID+KGRD+EL PFGGGRRICP L LA++ + LML SLL+ F
Sbjct: 412 PSVWENPSQFEPERFMGKDIDVKGRDYELTPFGGGRRICPGLPLAVKTVSLMLASLLYSF 471

Query: 380 DWKLEDGFRPDDLNMDEKYGLTLEMASPLRAFPL 413
           DWKL +G   +DL+MDE +G+TL   + L A P+
Sbjct: 472 DWKLPNGVVSEDLDMDETFGITLHRTNTLYAIPV 505

HSP 2 Score: 174.1 bits (440), Expect = 1.8e-43
Identity = 115/360 (31.94%), Postives = 188/360 (52.22%), Query Frame = 1

Query: 4   LGIFIVLLSIAWLISMISKTRPITN----LPPGPKPLPLIGNLLALGDKPHQSLANLAKS 63
           L +    +S  +LIS  +++R  +     LPPGP  LP+IGN+  +G  PH S A+LAK 
Sbjct: 9   LFLLFCFISSCFLISTTARSRRSSGRAATLPPGPPRLPIIGNIHQVGKNPHSSFADLAKI 68

Query: 64  YGPIMTLKLGQITTVVISSSAMAKQVLQTHDQFLSSRTVPDSMTTHNHDLFGLPWMPVSP 123
           YGPIM+LK G + +VVI+S   A++VL+THDQ LS R   DS+    H+   + W+P S 
Sbjct: 69  YGPIMSLKFGCLNSVVITSPEAAREVLRTHDQILSGRKSNDSIRCFGHEEVSVIWLPPSS 128

Query: 124 L-WRNLRRICNTQLFAARILDANENLRRGQVAELVTEISRCALKGNETVDFGKVAFVTSV 183
             WR LR++  T +F+ +  +A + LR  +V ELV+ ++  + +  E VD  + ++ T +
Sbjct: 129 ARWRMLRKLSVTLMFSPQRTEATKALRMKKVQELVSFMNESS-ERKEAVDISRASYTTVL 188

Query: 184 NLLWNTIFSEDFVDPNSKIGREFKVAVRGAMEEAAKPNFGDYFALLKRFDVQGIKKKQSV 243
           N++ N +FS D    +SK   EF+  V GAME A KP+  +YF  +   D+QG +K    
Sbjct: 189 NIISNILFSVDLGSYDSKKSNEFQDTVIGAMEAAGKPDAANYFPFMGFLDLQGNRKAMRG 248

Query: 244 HFDRIFDVLEQMIDERIDEQ---KKKSDISRLPYLQAVIKETLRCHSPPFLLPRKALQDV 303
             +R+F V    +D +I E+       D+S   +L +++   +        L    ++ +
Sbjct: 249 LTERLFRVFRGFMDAKIAEKSLGNYSKDVSNRDFLDSLL---ILNEGDEAELDNNDIEHL 308

Query: 304 EISGFTIPKDAQVPVNLWAMGRDSNVWKNPEIF-----EPERFLEMEIDIKGRDFELVPF 351
            +  FT   D       WAM   + + +NP+       E +R L     ++  D   +P+
Sbjct: 309 LLDMFTAGTDTSSSTLEWAM---AELLRNPKTMVKAQAEMDRVLGQNSVVQESDISGLPY 361

BLAST of Cucsa.302540 vs. TAIR10
Match: AT2G45560.1 (AT2G45560.1 cytochrome P450, family 76, subfamily C, polypeptide 1)

HSP 1 Score: 196.4 bits (498), Expect = 3.4e-50
Identity = 93/154 (60.39%), Postives = 117/154 (75.97%), Query Frame = 1

Query: 260 KKSDISRLPYLQAVIKETLRCHSP-PFLLPRKALQDVEISGFTIPKDAQVPVNLWAMGRD 319
           ++SDIS+LPYLQAV+KET R H+P P L+PRKA  D EI GF + KD QV VN+WA+GRD
Sbjct: 352 EESDISKLPYLQAVVKETFRLHTPVPLLIPRKAESDAEILGFMVLKDTQVLVNVWAIGRD 411

Query: 320 SNVWKNPEIFEPERFLEMEIDIKGRDFELVPFGGGRRICPELSLAMRMLPLMLGSLLHFF 379
            +VW NP  FEPERFL  ++D++GRD+EL PFG GRRICP + LAM+ + LML SLL+ F
Sbjct: 412 PSVWDNPSQFEPERFLGKDMDVRGRDYELTPFGAGRRICPGMPLAMKTVSLMLASLLYSF 471

Query: 380 DWKLEDGFRPDDLNMDEKYGLTLEMASPLRAFPL 413
           DWKL  G   +DL+MDE +GLTL   +PL A P+
Sbjct: 472 DWKLPKGVLSEDLDMDETFGLTLHKTNPLHAVPV 505

HSP 2 Score: 174.5 bits (441), Expect = 1.4e-43
Identity = 116/332 (34.94%), Postives = 180/332 (54.22%), Query Frame = 1

Query: 3   LLGIFIVLLSIAWLISMISK---TRPITNLPPGPKPLPLIGNLLALGDKPHQSLANLAKS 62
           LL +F  +LS   + +       +R  T LPPGP  LP+IGN+  +G  PH+S A L+K+
Sbjct: 9   LLLLFCFILSCFLIFTTTRSGRISRGATALPPGPPRLPIIGNIHLVGKHPHRSFAELSKT 68

Query: 63  YGPIMTLKLGQITTVVISSSAMAKQVLQTHDQFLSSRTVPDSMTTHNHDLFGLPWMPVSP 122
           YGP+M+LKLG + TVVI+S   A++VL+THDQ LS+R+  +++ + NH    L W+P S 
Sbjct: 69  YGPVMSLKLGSLNTVVIASPEAAREVLRTHDQILSARSPTNAVRSINHQDASLVWLPSSS 128

Query: 123 L-WRNLRRICNTQLFAARILDANENLRRGQVAELVTEISRCALKGNETVDFGKVAFVTSV 182
             WR LRR+  TQL + + ++A + LR  +V ELV+ IS  + +  E+VD  +VAF+T++
Sbjct: 129 ARWRLLRRLSVTQLLSPQRIEATKALRMNKVKELVSFISESSDR-EESVDISRVAFITTL 188

Query: 183 NLLWNTIFSEDFVDPNSKIG-REFKVAVRGAMEEAAKPNFGDYFALLKRFDVQGIKKKQS 242
           N++ N +FS D    N+K      +  V   M+ A  P+  +YF  L+  D+QG  K   
Sbjct: 189 NIISNILFSVDLGSYNAKASINGVQDTVISVMDAAGTPDAANYFPFLRFLDLQGNVKTFK 248

Query: 243 VHFDRIFDVLEQMIDERIDE---QKKKSDISRLPYLQAVIKETLRCHSPPFLLPRKALQD 302
           V  +R+  V    ID +I E   Q    D+S+  +    +   L        L    ++ 
Sbjct: 249 VCTERLVRVFRGFIDAKIAEKSSQNNPKDVSKNDF----VDNLLDYKGDESELSISDIEH 308

Query: 303 VEISGFTIPKDAQVPVNLWAMGRDSNVWKNPE 327
           + +  FT   D       WAM   + + KNP+
Sbjct: 309 LLLDMFTAGTDTSSSTLEWAM---TELLKNPK 332

BLAST of Cucsa.302540 vs. TAIR10
Match: AT1G33720.1 (AT1G33720.1 cytochrome P450, family 76, subfamily C, polypeptide 6)

HSP 1 Score: 192.6 bits (488), Expect = 4.9e-49
Identity = 98/172 (56.98%), Postives = 127/172 (73.84%), Query Frame = 1

Query: 242 RIFDVLEQMIDERIDEQKKKSDISRLPYLQAVIKETLRCH-SPPFLLPRKALQDVEISGF 301
           ++ D +  +I +  D Q+  SDIS+LPYL+AV+KET R H + PFLL RKA  +VEI GF
Sbjct: 336 KVQDEINHVIGQNGDFQE--SDISKLPYLKAVVKETFRLHPAAPFLLQRKAETNVEILGF 395

Query: 302 TIPKDAQVPVNLWAMGRDSNVWKNPEIFEPERFLEMEIDIKGRDFELVPFGGGRRICPEL 361
           T+ KD+QV VN+WA+GRD  VW+NP  FEPERFL  EID+KG D+EL PFG GRRICP L
Sbjct: 396 TVLKDSQVLVNVWAIGRDPLVWENPTHFEPERFLGKEIDVKGTDYELTPFGAGRRICPGL 455

Query: 362 SLAMRMLPLMLGSLLHFFDWKLEDGFRPDDLNMDEKYGLTLEMASPLRAFPL 413
            LAM+ + LML SLL+ F+WKL +G   +DL+M+E +GLT+   +PL A PL
Sbjct: 456 PLAMKTVHLMLASLLYTFEWKLPNGVGSEDLDMEETFGLTVHKTNPLLAVPL 505

HSP 2 Score: 169.1 bits (427), Expect = 5.8e-42
Identity = 98/276 (35.51%), Postives = 159/276 (57.61%), Query Frame = 1

Query: 6   IFIVLLS-IAWLISMISKTRP--ITNLPPGPKPLPLIGNLLALGDKPHQSLANLAKSYGP 65
           IF  +LS + +  +  S+  P  ++  PPGP  LP+IGN+  +G  PH S  +L+K+YGP
Sbjct: 12  IFCFILSCLLFFTTARSRRSPCQLSKSPPGPPRLPIIGNIHLVGKNPHHSFTDLSKTYGP 71

Query: 66  IMTLKLGQITTVVISSSAMAKQVLQTHDQFLSSRTVPDSMTTHNHDLFGLPWM-PVSPLW 125
           +M+LKLG + +VVI+S    ++VL+THDQ LS R + ++  ++NH  F + W+ P S  +
Sbjct: 72  VMSLKLGCLNSVVIASRDAVREVLKTHDQILSGRYISEATKSNNHHEFSVGWIHPSSSRF 131

Query: 126 RNLRRICNTQLFAARILDANENLRRGQVAELVTEISRCALKGNETVDFGKVAFVTSVNLL 185
           R LR++  TQLF+ + + A + LR  +V ELV  +S  + +  E VD   V+FVT++N++
Sbjct: 132 RMLRKLSATQLFSPQCIQATKALRMKKVQELVNFLSE-SCEREEAVDISHVSFVTALNII 191

Query: 186 WNTIFSEDFVDPNSKIGREFKVAVRGAMEEAAKPNFGDYFALLKRFDVQGIKKKQSVHFD 245
            N +FS +    +SK    F+  V G  E    P+  ++F  ++  D+QG  KK      
Sbjct: 192 SNILFSVNLGSYDSKNSSAFQEMVIGYQESIGNPDLANFFPFMRFLDLQGNSKKMRESSG 251

Query: 246 RIFDVLEQMIDERIDEQKKKS---DISRLPYLQAVI 275
           R+  V  +  D RI E+  +S   D+S   +L  +I
Sbjct: 252 RLLQVFREFYDARIVEKSSRSVEKDVSSKDFLDVLI 286

BLAST of Cucsa.302540 vs. TAIR10
Match: AT1G33730.1 (AT1G33730.1 cytochrome P450, family 76, subfamily C, polypeptide 5)

HSP 1 Score: 190.3 bits (482), Expect = 2.4e-48
Identity = 95/169 (56.21%), Postives = 126/169 (74.56%), Query Frame = 1

Query: 242 RIFDVLEQMIDERIDEQKKKSDISRLPYLQAVIKETLRCH-SPPFLLPRKALQDVEISGF 301
           ++ D + ++I +  D Q+  S IS+LPYLQAVIKET R H + PFLLPRKA +DV+I GF
Sbjct: 200 KVQDEINRVIRQNGDVQE--SHISKLPYLQAVIKETFRLHPAAPFLLPRKAERDVDILGF 259

Query: 302 TIPKDAQVPVNLWAMGRDSNVWKNPEIFEPERFLEMEIDIKGRDFELVPFGGGRRICPEL 361
            +PKD+ V VN+WA+GRD NVW+NP  FEPERFL  +ID+KG ++EL PFG GRRICP L
Sbjct: 260 HVPKDSHVLVNVWAIGRDPNVWENPTQFEPERFLGKDIDVKGTNYELTPFGAGRRICPGL 319

Query: 362 SLAMRMLPLMLGSLLHFFDWKLEDGFRPDDLNMDEKYGLTLEMASPLRA 410
            LA++ + LML SLL+ F+WKL +G   +DL+M E +GLT+   +PL A
Sbjct: 320 PLALKTVHLMLASLLYTFEWKLPNGVGSEDLDMGETFGLTVHKTNPLLA 366

HSP 2 Score: 80.1 bits (196), Expect = 3.5e-15
Identity = 47/148 (31.76%), Postives = 80/148 (54.05%), Query Frame = 1

Query: 130 TQLFAARILDANENLRRGQVAELVTEISRCALKGNETVDFGKVAFVTSVNLLWNTIFSED 189
           TQLF+ + ++A + LR  +V ELV  +S  + +G E VD  + +FVT++N++ N +FS +
Sbjct: 4   TQLFSPQRIEATKALRMKKVQELVNFLSESSERG-EAVDISRASFVTALNIISNILFSVN 63

Query: 190 FVDPNSKIGREFKVAVRGAMEEAAKPNFGDYFALLKRFDVQGIKKKQSVHFDRIFDVLEQ 249
               +SK    F+  V G ME    P+  ++F  ++  D+QG  KK   +  ++  V  +
Sbjct: 64  LGSYDSKNSSAFQEMVIGYMESIGNPDVSNFFPFMRLLDLQGNSKKMKEYSGKLLQVFRE 123

Query: 250 MIDERIDEQKKK---SDISRLPYLQAVI 275
             D RI E   +    D+S   +L A+I
Sbjct: 124 FYDARILENSSRIDEKDVSSRDFLDALI 150

BLAST of Cucsa.302540 vs. NCBI nr
Match: gi|778699836|ref|XP_004147598.2| (PREDICTED: geraniol 8-hydroxylase-like [Cucumis sativus])

HSP 1 Score: 508.1 bits (1307), Expect = 1.5e-140
Identity = 255/260 (98.08%), Postives = 257/260 (98.85%), Query Frame = 1

Query: 1   MDLLGIFIVLLSIAWLISMISKTRPITNLPPGPKPLPLIGNLLALGDKPHQSLANLAKSY 60
           MDLL IFIVLLSIAWLISMISKTRPITNLPPGPKPLPLIGNLLALGDKPHQSLANLAKSY
Sbjct: 1   MDLLWIFIVLLSIAWLISMISKTRPITNLPPGPKPLPLIGNLLALGDKPHQSLANLAKSY 60

Query: 61  GPIMTLKLGQITTVVISSSAMAKQVLQTHDQFLSSRTVPDSMTTHNHDLFGLPWMPVSPL 120
           GPIMTLKLGQITTVVISSSAMAKQVLQTHDQFLSSRTVPDSMTTHNHDLFGLPWMPVSPL
Sbjct: 61  GPIMTLKLGQITTVVISSSAMAKQVLQTHDQFLSSRTVPDSMTTHNHDLFGLPWMPVSPL 120

Query: 121 WRNLRRICNTQLFAARILDANENLRRGQVAELVTEISRCALKGNETVDFGKVAFVTSVNL 180
           WRNLRRICNTQLFAARILDANENLRRGQVAELVTEISRCALKG+E VDFGKVAFVTS+NL
Sbjct: 121 WRNLRRICNTQLFAARILDANENLRRGQVAELVTEISRCALKGDEAVDFGKVAFVTSMNL 180

Query: 181 LWNTIFSEDFVDPNSKIGREFKVAVRGAMEEAAKPNFGDYFALLKRFDVQGIKKKQSVHF 240
           LWNTIFSEDFVDPNSKIGREFKVAVRGAMEEAAKPNFGDYF LLKRFDVQGIKKKQSVHF
Sbjct: 181 LWNTIFSEDFVDPNSKIGREFKVAVRGAMEEAAKPNFGDYFPLLKRFDVQGIKKKQSVHF 240

Query: 241 DRIFDVLEQMIDERIDEQKK 261
           DRIFDVLEQMIDERIDEQKK
Sbjct: 241 DRIFDVLEQMIDERIDEQKK 260

BLAST of Cucsa.302540 vs. NCBI nr
Match: gi|778699836|ref|XP_004147598.2| (PREDICTED: geraniol 8-hydroxylase-like [Cucumis sativus])

HSP 1 Score: 294.3 bits (752), Expect = 3.4e-76
Identity = 144/155 (92.90%), Postives = 147/155 (94.84%), Query Frame = 1

Query: 260 KKSDISRLPYLQAVIKETLRCHSPPFLLPRKALQDVEISGFTIPKDAQVPVNLWAMGRDS 319
           K+SDISRLPYLQAVIKETLR HSPPFLLPRKALQDVEIS FTIPKDAQV VNLWAM RDS
Sbjct: 343 KESDISRLPYLQAVIKETLRYHSPPFLLPRKALQDVEISSFTIPKDAQVLVNLWAMSRDS 402

Query: 320 NVWKNPEIFEPERFLEMEIDIKGRDFELVPFGGGRRICPELSLAMRMLPLMLGSLLHFFD 379
           NVWKNPEIFEPERFLEM+IDIKGRDFELVPFGGGRRICP L LAMRMLPLMLGSLLHFFD
Sbjct: 403 NVWKNPEIFEPERFLEMDIDIKGRDFELVPFGGGRRICPGLPLAMRMLPLMLGSLLHFFD 462

Query: 380 WKLEDGFRPDDLNMDEKYGLTLEMASPLRAFPLLV 415
           WKLEDG RPDDLNMDEKYG+TL MASPLRAFPLLV
Sbjct: 463 WKLEDGCRPDDLNMDEKYGITLAMASPLRAFPLLV 497

HSP 2 Score: 484.6 bits (1246), Expect = 1.8e-133
Identity = 241/251 (96.02%), Postives = 246/251 (98.01%), Query Frame = 1

Query: 19  MISKTRPITNLPPGPKPLPLIGNLLALGDKPHQSLANLAKSYGPIMTLKLGQITTVVISS 78
           MISKTRPITNLPPGPKPLPLIGNLLALGDKPHQSLANLAKSYGPIMTLKLGQITTVVISS
Sbjct: 1   MISKTRPITNLPPGPKPLPLIGNLLALGDKPHQSLANLAKSYGPIMTLKLGQITTVVISS 60

Query: 79  SAMAKQVLQTHDQFLSSRTVPDSMTTHNHDLFGLPWMPVSPLWRNLRRICNTQLFAARIL 138
           SAMAKQVLQTHDQFLSSRTVPDSMTTHNHDLFGLPWMPVSPLWRNLRRICNTQLFAARIL
Sbjct: 61  SAMAKQVLQTHDQFLSSRTVPDSMTTHNHDLFGLPWMPVSPLWRNLRRICNTQLFAARIL 120

Query: 139 DANENLRRGQVAELVTEISRCALKGNETVDFGKVAFVTSVNLLWNTIFSEDFVDPNSKIG 198
           DANENLRRGQVAELVTEISRCALKGNETVDFGKVAFVTS+NLLWNTIFSEDFVDPNSKIG
Sbjct: 121 DANENLRRGQVAELVTEISRCALKGNETVDFGKVAFVTSMNLLWNTIFSEDFVDPNSKIG 180

Query: 199 REFKVAVRGAMEEAAKPNFGDYFALLKRFDVQGIKKKQSVHFDRIFDVLEQMIDERIDEQ 258
           REFKVAVRGAMEEAAKPNFGDYFALLKRFDVQGIKKKQSVHFDRIFDVLEQMIDERIDEQ
Sbjct: 181 REFKVAVRGAMEEAAKPNFGDYFALLKRFDVQGIKKKQSVHFDRIFDVLEQMIDERIDEQ 240

Query: 259 KKKSDISRLPY 270
           KK    +++ +
Sbjct: 241 KKSCGSNKIKH 251

BLAST of Cucsa.302540 vs. NCBI nr
Match: gi|659073652|ref|XP_008437178.1| (PREDICTED: geraniol 8-hydroxylase-like [Cucumis melo])

HSP 1 Score: 481.1 bits (1237), Expect = 2.0e-132
Identity = 239/269 (88.85%), Postives = 252/269 (93.68%), Query Frame = 1

Query: 1   MDLLGIFIVLLSIAWLISMISKTRPITNLPPGPKPLPLIGNLLALGDKPHQSLANLAKSY 60
           MDLL IFIVLLSI WLISMISKTR I  LPPGPKPLPLIGNLLALGD+PH+SLANLAKSY
Sbjct: 1   MDLLWIFIVLLSITWLISMISKTRTIAKLPPGPKPLPLIGNLLALGDQPHKSLANLAKSY 60

Query: 61  GPIMTLKLGQITTVVISSSAMAKQVLQTHDQFLSSRTVPDSMTTHNHDLFGLPWMPVSPL 120
           GPIMTLKLGQ+TTVVISSSAMAKQVLQTHDQFLSSRTVPDSMTTHNHDLFGLPWMPVSPL
Sbjct: 61  GPIMTLKLGQVTTVVISSSAMAKQVLQTHDQFLSSRTVPDSMTTHNHDLFGLPWMPVSPL 120

Query: 121 WRNLRRICNTQLFAARILDANENLRRGQVAELVTEISRCALKGNETVDFGKVAFVTSVNL 180
           WRNLRR+CNTQL A RILDANENLRRGQV ELVTEISRCALKG+E VDFGKVAFVT+VNL
Sbjct: 121 WRNLRRVCNTQLLAPRILDANENLRRGQVEELVTEISRCALKGDEAVDFGKVAFVTTVNL 180

Query: 181 LWNTIFSEDFVDPNSKIGREFKVAVRGAMEEAAKPNFGDYFALLKRFDVQGIKKKQSVHF 240
           LWNTIFSEDFVDPNSKIGREFKVAVRG MEEA KPNFGDYF LLKRFDVQGIKK+QSV+F
Sbjct: 181 LWNTIFSEDFVDPNSKIGREFKVAVRGIMEEAVKPNFGDYFPLLKRFDVQGIKKRQSVYF 240

Query: 241 DRIFDVLEQMIDERIDEQKKKSDISRLPY 270
           DRIFDVLEQ+IDERIDEQKK    +++ +
Sbjct: 241 DRIFDVLEQIIDERIDEQKKSFGSNKIKH 269

BLAST of Cucsa.302540 vs. NCBI nr
Match: gi|659073652|ref|XP_008437178.1| (PREDICTED: geraniol 8-hydroxylase-like [Cucumis melo])

HSP 1 Score: 293.5 bits (750), Expect = 5.8e-76
Identity = 142/155 (91.61%), Postives = 148/155 (95.48%), Query Frame = 1

Query: 260 KKSDISRLPYLQAVIKETLRCHSPPFLLPRKALQDVEISGFTIPKDAQVPVNLWAMGRDS 319
           K+SDISRLPYLQAVIKETLR HSPPFLLPRKAL+DVEISGFTIPKDAQV VNLWAMGRDS
Sbjct: 343 KESDISRLPYLQAVIKETLRLHSPPFLLPRKALEDVEISGFTIPKDAQVLVNLWAMGRDS 402

Query: 320 NVWKNPEIFEPERFLEMEIDIKGRDFELVPFGGGRRICPELSLAMRMLPLMLGSLLHFFD 379
           N WKNPE+FEPERFLEMEIDIKGR+FELVPFG GRRICP LSLAMRMLPLMLGS+LHFFD
Sbjct: 403 NAWKNPEVFEPERFLEMEIDIKGRNFELVPFGAGRRICPGLSLAMRMLPLMLGSVLHFFD 462

Query: 380 WKLEDGFRPDDLNMDEKYGLTLEMASPLRAFPLLV 415
           WKLEDG  PDDLNMDEKYG+TLEMASPLRAFPLLV
Sbjct: 463 WKLEDGCGPDDLNMDEKYGITLEMASPLRAFPLLV 497

HSP 2 Score: 420.6 bits (1080), Expect = 3.1e-114
Identity = 214/438 (48.86%), Postives = 289/438 (65.98%), Query Frame = 1

Query: 1   MDLLGIFIVLLSIAWLISMI------SKTRPITNLPPGPKPLPLIGNLLALGDKPHQSLA 60
           MDLL   ++ L   W +          +      LPPGP P P++GNL  LGDKPH+SLA
Sbjct: 1   MDLLTSSLLCLLFTWFLLQAFNSFRNGRKSSQRKLPPGPWPKPIVGNLFDLGDKPHRSLA 60

Query: 61  NLAKSYGPIMTLKLGQITTVVISSSAMAKQVLQTHDQFLSSRTVPDSMTTHNHDLFGLPW 120
            LA+ +GP+M+LKLG I T+V+SS   AK++LQ  D    +RT+ D++    H   GLPW
Sbjct: 61  KLAQIHGPVMSLKLGSILTIVVSSETTAKEILQKQDLTFCNRTIVDAVRASQHYEVGLPW 120

Query: 121 MPVSPLWRNLRRICNTQLFAARILDANENLRRGQVAELVTEISRCALKGNETVDFGKVAF 180
           +PVSPLWR LR++C T +FA+  LDANE LR+ ++ EL+  +    LKG E V+ G+VAF
Sbjct: 121 IPVSPLWRTLRKVCKTHIFASIKLDANEYLRQNKIQELIANVGESCLKG-EAVNIGQVAF 180

Query: 181 VTSVNLLWNTIFSEDFVDPNSKIGREFKVAVRGAMEEAAKPNFGDYFALLKRFDVQGI-- 240
            T++NLL NT+FS D VDPNS I REFK  VRG M+E   PN  D+F  L++ D QG+  
Sbjct: 181 DTTINLLSNTVFSMDLVDPNSSIAREFKKTVRGMMDEVGTPNLADFFPFLRKIDPQGVLF 240

Query: 241 -------------KKKQSVHFDRIFDVLEQMIDERIDEQK--KKSDISRLPYLQAVIKET 300
                           + +   ++    ++ +++ I++ K  ++SDI+ LPYLQA+IKET
Sbjct: 241 VAGTDTTSSTLEWAMAELLQNPQVLLKAKKELNQAIEKGKPIEESDINHLPYLQAIIKET 300

Query: 301 LRCHSP-PFLLPRKALQDVEISGFTIPKDAQVPVNLWAMGRDSNVWKNPEIFEPERFLEM 360
            R H   P LLPR+A  D E+ GF +PK +QV VN+WA+GRD ++WKNP  F PERFL  
Sbjct: 301 FRMHPVVPLLLPRRAGSDAELCGFKVPKGSQVLVNVWAIGRDPSIWKNPNSFMPERFLGS 360

Query: 361 EIDIKGRDFELVPFGGGRRICPELSLAMRMLPLMLGSLLHFFDWKLEDGFRPDDLNMDEK 415
           EID+KGRDF L+PFG GRRICP L LA RML LMLGSL++ FDWKLE G  P+++NM+EK
Sbjct: 361 EIDVKGRDFGLIPFGAGRRICPGLPLANRMLHLMLGSLINSFDWKLEGGISPNEINMEEK 420

BLAST of Cucsa.302540 vs. NCBI nr
Match: gi|764646292|ref|XP_011457550.1| (PREDICTED: geraniol 8-hydroxylase-like [Fragaria vesca subsp. vesca])

HSP 1 Score: 412.1 bits (1058), Expect = 1.1e-111
Identity = 222/464 (47.84%), Postives = 299/464 (64.44%), Query Frame = 1

Query: 1   MDLL-GIFIVLLSIAWLISMISKTRPITNLPPGPKPLPLIGNLLALGDKPHQSLANLAKS 60
           MD L GI + L  I+  +      R    LPPGPKP PL+GNL  LGDKPH SL NL++ 
Sbjct: 1   MDFLSGILLFLCLISCFLIQALHRRTRVRLPPGPKPFPLVGNLFDLGDKPHISLTNLSQH 60

Query: 61  YGPIMTLKLGQITTVVISSSAMAKQVLQTHDQFLSSRTVPDSMTTHNHDLFGLPWMPVSP 120
           YG IM+LKLGQITTVV+SS  MAK++L+ +D    +RT+PD++     +   LPWMPVSP
Sbjct: 61  YGSIMSLKLGQITTVVVSSPTMAKEILRIYDHLFCNRTIPDALRVLIDN--SLPWMPVSP 120

Query: 121 LWRNLRRICNTQLFAARILDANENLRRGQVAELVTEISRCALKGNETVDFGKVAFVTSVN 180
            WRNLR+IC +QLFA R+LDAN+  RR +V EL+  ++   +KG   VD GK AF+TS+N
Sbjct: 121 RWRNLRKICTSQLFATRVLDANKENRREKVQELIASVNDNMIKGL-AVDIGKAAFITSLN 180

Query: 181 LLWNTIFSEDFVDPNS----KIGREFKVAVRGAMEEAAKPNFGDYFALLKRFDVQGIKKK 240
           LL  TIFS D  D +S    +I +EFK    G +EEA KPN GD+F +LK+ D QG +++
Sbjct: 181 LLSRTIFSVDLADDDSSSSNEITKEFKKISTGMLEEAGKPNLGDFFPVLKKIDPQGRRRR 240

Query: 241 QSVHFDRIF-----------------------DVLEQMIDERIDEQKK------------ 300
            + H++++                        D+L+ +ID R +E ++            
Sbjct: 241 FTNHYNKMLGLFESVIKQRLEFRKGDDYVASNDMLDTLIDLREEENEEDLNNPKVIEHLL 300

Query: 301 ----------KSDISRLPYLQAVIKETLRCHS-PPFLLPRKALQDVEISGFTIPKDAQVP 360
                     +S+I+RLPYLQA+IKET R     P LLPRKA  DVEI G+ +PK AQV 
Sbjct: 301 SALFVAGTEQESNIARLPYLQAIIKETFRLRPVAPLLLPRKAETDVEIGGYIVPKGAQVL 360

Query: 361 VNLWAMGRDSNVWKNPEIFEPERFL--EMEIDIKGRDFELVPFGGGRRICPELSLAMRML 412
           VN+WA+GRD ++W NP  F PERFL  + +ID+ G +FEL+PFG GRRICP L LAMRML
Sbjct: 361 VNIWAIGRDPSIWDNPNSFMPERFLGSDNQIDVMGNNFELIPFGSGRRICPGLPLAMRML 420