BLAST of Cucsa.299750 vs. Swiss-Prot
Match:
MSL10_ARATH (Mechanosensitive ion channel protein 10 OS=Arabidopsis thaliana GN=MSL10 PE=1 SV=1)
HSP 1 Score: 706.4 bits (1822), Expect = 3.1e-202
Identity = 387/740 (52.30%), Postives = 504/740 (68.11%), Query Frame = 1
Query: 14 SQKESGNGGEVVVQLSGVENECSVPKQNRADSQTKEPTGSSVGYDNASPLAPTPNKPPKI 73
S G GG+VV+ + VE K+ + K S S L +PNKPP+
Sbjct: 6 SSNGGGGGGDVVINVP-VEEASRRSKEMASPESEKGVPFSKSPSPEISKLVGSPNKPPRA 65
Query: 74 PISNGT-LTPRRSLRRSTLSKPKSRFGEQSCFIDSDMLEEENHVSLREQIGATSSRSSNL 133
P N LT R+S RS SKPKSRF + SC +D+ +LEEE +REQ+GA S S
Sbjct: 66 PNQNNVGLTQRKSFARSVYSKPKSRFVDPSCPVDTSILEEE----VREQLGAGFSFS--- 125
Query: 134 NTPKAQPEGEDDGNMGSNAK----------------HKKVKVK----------TVIKWIG 193
+A P + + ++GS A +KKVK+ +I+
Sbjct: 126 ---RASPNNKSNRSVGSPAPVTPSKVVVEKDEDEEIYKKVKLNREMRSKISTLALIESAF 185
Query: 194 VFCIISCLVASLTVNPLKNRFLWGLKVWKWCLLATVILCGLIFTRWVMNVVVFLIERNFL 253
I+S LVASLT+N LK+ WGL+VWKWC+L VI G++ T W M ++VFLIE NFL
Sbjct: 186 FVVILSALVASLTINVLKHHTFWGLEVWKWCVLVMVIFSGMLVTNWFMRLIVFLIETNFL 245
Query: 254 LKKKVLYFVHGLKKSVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASF 313
L++KVLYFVHGLKKSVQV +WL L+L W LF NH + S K+L +T TL S
Sbjct: 246 LRRKVLYFVHGLKKSVQVFIWLCLILVAWILLF---NHDVKRSPAATKVLKCITRTLISI 305
Query: 314 LIGAFLWLIKTLLLKILASKFHMNRFFDRIQESLFLHHVLQTLLRPPLVED------EST 373
L GAF WL+KTLLLKILA+ F++N FFDRIQ+S+F +VLQTL PL+E+ E +
Sbjct: 306 LTGAFFWLVKTLLLKILAANFNVNNFFDRIQDSVFHQYVLQTLSGLPLMEEAERVGREPS 365
Query: 374 AKFRCCRFCFESKKPDRKKVIDMGKIHELKREKVSAWTMKVLVDAVTSSEMS-VSQILDD 433
+ KKVIDMGK+H++KREKVSAWTM+VL++AV +S +S +S LD+
Sbjct: 366 TGHLSFATVVKKGTVKEKKVIDMGKVHKMKREKVSAWTMRVLMEAVRTSGLSTISDTLDE 425
Query: 434 ESY---RDVADGDITNEMKVAKEAAKEIFKNVALPGNKFIEERDLLEFMIPEEVNLVWPH 493
+Y ++ AD +IT+EM+ A AA +F+NVA P +IEE DLL FMI EEV+LV+P
Sbjct: 426 TAYGEGKEQADREITSEME-ALAAAYHVFRNVAQPFFNYIEEEDLLRFMIKEEVDLVFPL 485
Query: 494 FE-VDKTRKIDMKALTNWVVKVYQGRKTLAHALKDTKTAVKQLNNLITALIIVVTAVIWL 553
F+ +T +I KA T WVVKVY R+ LAH+L DTKTAVKQLN L+TA+++VVT VIWL
Sbjct: 486 FDGAAETGRITRKAFTEWVVKVYTSRRALAHSLNDTKTAVKQLNKLVTAILMVVTVVIWL 545
Query: 554 LLMEIATTKVLVFLLTQLAVAAFMFGNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLV 613
LL+E+ATTKVL+F TQL AF+ G+TCK FE+++FVFVMHP+DVGDRCVVDGV +LV
Sbjct: 546 LLLEVATTKVLLFFSTQLVALAFIIGSTCKNLFESIVFVFVMHPYDVGDRCVVDGVAMLV 605
Query: 614 EEMNILTTVFLKLNNEKVYYPNSVLATKPITNYYRSPDMGDTVEFSIGFETPVERIGAMK 673
EEMN+LTTVFLKLNNEKVYYPN+VLATKPI+NY+RSP+MG+TVEFSI F TPV +I +K
Sbjct: 606 EEMNLLTTVFLKLNNEKVYYPNAVLATKPISNYFRSPNMGETVEFSISFSTPVSKIAHLK 665
Query: 674 EQIKRYLEENPQHWYPNHNVVVKEIENVNKIKIALYTNHTMNFQDWAEKNQRRTKLVMEL 716
E+I YLE+NPQHW P H+VVVKEIEN+NK+K+ALY++HT+ FQ+ E+N RRT+L + +
Sbjct: 666 ERIAEYLEQNPQHWAPVHSVVVKEIENMNKLKMALYSDHTITFQENRERNLRRTELSLAI 725
BLAST of Cucsa.299750 vs. Swiss-Prot
Match:
MSL9_ARATH (Mechanosensitive ion channel protein 9 OS=Arabidopsis thaliana GN=MSL9 PE=1 SV=1)
HSP 1 Score: 648.3 bits (1671), Expect = 1.0e-184
Identity = 356/748 (47.59%), Postives = 487/748 (65.11%), Query Frame = 1
Query: 14 SQKESGNGGEVVVQLSGVENECSVPKQNRADSQTKEPTGSSVGYDNASPLAP----TP-- 73
+++ NG EVV+ +S E+ P+ + + + P S G + + P+ P TP
Sbjct: 2 AERRVSNGEEVVINVSDKEDSKD-PRASPSFNPLASPD-SDAGIEKSKPVPPISIPTPEI 61
Query: 74 -------NKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQSCFIDSDMLEEENHVSLRE 133
+KPPKIP G L R+SL RS SKPKSRFGEQ F EE SLRE
Sbjct: 62 YKFSGSVHKPPKIPSPEG-LVRRKSLSRSIYSKPKSRFGEQQSFRYDSTREENGGRSLRE 121
Query: 134 QIGATSSRSSNLNTPKAQPEGEDDGNMGSNAK--------------HKKVKVKTV----- 193
Q GA S + + +A P + + ++ S A +KKVK+ V
Sbjct: 122 QFGAGSFARGSFD--RASPNNKSNRSVASAALSKVAEEEPDENEEIYKKVKLHRVKRSGM 181
Query: 194 -----IKWIGVFCIISCLVASLTVNPLKNRFLWGLKVWKWCLLATVILCGLIFTRWVMNV 253
++ + I+ L+ SLT++ + +WGL+ WKWC+L V L G++ T W M+
Sbjct: 182 KPLAFLELVVFMAILGALIVSLTIDVVNKHTIWGLEFWKWCVLVMVTLSGMLVTNWFMHF 241
Query: 254 VVFLIERNFLLKKKVLYFVHGLKKSVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKIL 313
VVF+IE+N+LL+KKVLYFVHGLKK+VQV +W SLVL W LFD + +R T + L
Sbjct: 242 VVFIIEKNYLLRKKVLYFVHGLKKNVQVFIWFSLVLIAWICLFDGD---VKRTRKTKRFL 301
Query: 314 DAVTWTLASFLIGAFLWLIKTLLLKILASKFHMNRFFDRIQESLFLHHVLQTLLRPPLVE 373
D +TWT+ S L+G+ L+L+KT LK+LASKF++ FF+RIQES+F +VLQTL PPL+E
Sbjct: 302 DFITWTIVSLLVGSILFLVKTFALKVLASKFNVRNFFERIQESVFHQYVLQTLSGPPLIE 361
Query: 374 DE-------STAKFRCCRFCFESKKPDRKKVIDMGKIHELKREKVSAWTMKVLVDAVTSS 433
+ ST R + K KKVIDMGK+H +K+EKVSAWTM+VL++AV +S
Sbjct: 362 EAENVGRVPSTGHLSFTRT--KDGKVKDKKVIDMGKVHRMKQEKVSAWTMRVLIEAVGTS 421
Query: 434 EMS-VSQILDD-ESYRDVADGDITNEMKVAKEAAKEIFKNVALPGNKFIEERDLLEFMIP 493
+S +S LD+ + ++ D +ITNEM+ A AA ++F NVA P + +IEE DLL FMI
Sbjct: 422 GISTISSTLDEVNNKKERTDKEITNEME-AVAAAYDVFNNVAKPNHNYIEEDDLLRFMIK 481
Query: 494 EEVNLVWPHFEVDKTRKIDMKALTNWVVKVYQGRKTLAHALKDTKTAVKQLNNLITALII 553
EEV+LV P E T KI K T WVV VY RKT+ H+L DTKTAVKQL+ LIT ++
Sbjct: 482 EEVDLVLPLIEDADTGKITRKTFTEWVVNVYTSRKTIGHSLNDTKTAVKQLDKLITGILT 541
Query: 554 VVTAVIWLLLMEIATTKVLVFLLTQLAVAAFMFGNTCKTAFEALIFVFVMHPFDVGDRCV 613
V+T ++W++L++IA+TK+L+ +Q AFM G+TCK FE+ +FVFVMHP+DVGDRCV
Sbjct: 542 VITFIVWMVLLDIASTKLLLVFSSQFLGLAFMIGSTCKNIFESFMFVFVMHPYDVGDRCV 601
Query: 614 VDGVQLLVEEMNILTTVFLKLNNEKVYYPNSVLATKPITNYYRSPDMGDTVEFSIGFETP 673
VDGV LLVEE+++LTTVFLK++NEKV+YPNSVL +KPI+N+YRSPDMGD V+F I F TP
Sbjct: 602 VDGVMLLVEEIDLLTTVFLKIDNEKVFYPNSVLISKPISNFYRSPDMGDYVDFGIAFSTP 661
Query: 674 VERIGAMKEQIKRYLEENPQHWYPNHNVVVKEIENVNKIKIALYTNHTMNFQDWAEKNQR 716
E+IG +K +I YL N QHWYP V+V+ IEN+NK+ + + HT+NFQ + EK+ R
Sbjct: 662 AEKIGCLKGKIGEYLVANSQHWYPEAQVMVRAIENMNKLVLNILVQHTINFQVYVEKSLR 721
BLAST of Cucsa.299750 vs. Swiss-Prot
Match:
MSL5_ARATH (Mechanosensitive ion channel protein 5 OS=Arabidopsis thaliana GN=MSL5 PE=2 SV=1)
HSP 1 Score: 473.0 bits (1216), Expect = 5.8e-132
Identity = 255/618 (41.26%), Postives = 375/618 (60.68%), Query Frame = 1
Query: 138 QPEGED---DGNMGSNAKHKKVKVKTVIKWIGVFCIISCLVASLTVNPLKNRFLWGLKVW 197
+ E ED D ++ K K+ ++WI + I++ LV SLT++ L+ + W L +W
Sbjct: 244 EEEEEDPFLDEDLPEEFKRDKLSFWVFLEWISLVLIVTSLVCSLTIHNLQRKTWWKLDLW 303
Query: 198 KWCLLATVILCGLIFTRWVMNVVVFLIERNFLLKKKVLYFVHGLKKSVQVTLWLSLVLAT 257
KW + V++CG + + W++ ++VFL+E+NF +K+VLYFV+G++KSVQ LWL LVL
Sbjct: 304 KWEVTVLVLICGRLVSSWIVRIIVFLVEKNFTWRKRVLYFVYGVRKSVQNCLWLGLVLLA 363
Query: 258 WGSLFDRRNHMISSSRITAKILDAVTWTLASFLIGAFLWLIKTLLLKILASKFHMNRFFD 317
W LFD++ + +R TA L VT L L+ +WL+KT+L+K+LAS FHM+ +FD
Sbjct: 364 WHFLFDKK--VERETRSTA--LRYVTRVLVCLLVALIIWLVKTILVKVLASSFHMSTYFD 423
Query: 318 RIQESLFLHHVLQTLLRPPLVEDES-------------------------TAKFRCCRFC 377
RIQESLF +V++TL PPL+E + K F
Sbjct: 424 RIQESLFTQYVIETLSGPPLMEIQRMEEEEQQVAEDVKSLEKLAGAKLPPALKATVKSFM 483
Query: 378 FESKKPDRKKV----------IDMGKIHELKREKVSAWTMKVLVDAVTSSEMS-VSQILD 437
K P ++ I + ++ + + VSAW MK L++ + +S + Q +
Sbjct: 484 KVGKSPGLNRIGSKRGEDGEGIRIDQLKRMNTKNVSAWNMKRLMNIILKGAISTLDQNMQ 543
Query: 438 DESYRDVADGDITNEMKVAKEAAKEIFKNVALPGNKFIEERDLLEFMIPEEVNLVWPHFE 497
D + D I +E + AK AA++IF NV PG+++I D L F+ EE FE
Sbjct: 544 DTTQEDEDATHIRSEYE-AKCAARKIFHNVTEPGSRYIYLEDFLRFLCEEEAERAMALFE 603
Query: 498 -VDKTRKIDMKALTNWVVKVYQGRKTLAHALKDTKTAVKQLNNLITALIIVVTAVIWLLL 557
++ KI L NWVVK ++ R+ LA L DTKTAV +L+ +I +I ++ +IWLL+
Sbjct: 604 GASESDKISKSCLKNWVVKAFRERRALALTLNDTKTAVDRLHRIINVVIGIIIIIIWLLI 663
Query: 558 MEIATTKVLVFLLTQLAVAAFMFGNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEE 617
+ IATT+ L+ L +QL + AF+FGN+CKT FEA+IF+FVMHPFDVGDRC +DGVQL+VEE
Sbjct: 664 LGIATTRFLLVLSSQLLLVAFVFGNSCKTIFEAIIFLFVMHPFDVGDRCEIDGVQLVVEE 723
Query: 618 MNILTTVFLKLNNEKVYYPNSVLATKPITNYYRSPDMGDTVEFSIGFETPVERIGAMKEQ 677
MNILTTVFL+ +N+K+ YPNSVL TKPI NYYRSPDMGD VEF + TP E+I A+K++
Sbjct: 724 MNILTTVFLRYDNQKIIYPNSVLGTKPIANYYRSPDMGDAVEFCVHIATPPEKITAIKQR 783
Query: 678 IKRYLEENPQHWYPNHNVVVKEIENVNKIKIALYTNHTMNFQDWAEKNQRRTKLVMELKR 716
I Y++ +WYP +V ++++N +KIA++ H MN QD E+ RR L+ E+ +
Sbjct: 784 ILSYVDNKKDYWYPAPMIVFLSMDDLNSVKIAVWLTHRMNHQDMGERYIRRGLLLEEVGK 843
BLAST of Cucsa.299750 vs. Swiss-Prot
Match:
MSL6_ARATH (Mechanosensitive ion channel protein 6 OS=Arabidopsis thaliana GN=MSL6 PE=1 SV=1)
HSP 1 Score: 471.9 bits (1213), Expect = 1.3e-131
Identity = 255/627 (40.67%), Postives = 378/627 (60.29%), Query Frame = 1
Query: 134 TPKAQPEGEDDG-----NMGSNAKHKKVKVKTVIKWIGVFCIISCLVASLTVNPLKNRFL 193
+PK Q E E+D ++ + K+ + V++W+ + II+ V +L + L+ + L
Sbjct: 211 SPKTQGEEEEDDPFAAEDLPEEYRKDKLSLWIVLEWLSLILIIAGFVCTLAIPSLRKKKL 270
Query: 194 WGLKVWKWCLLATVILCGLIFTRWVMNVVVFLIERNFLLKKKVLYFVHGLKKSVQVTLWL 253
W L++WKW + V++CG + + W++ +VVF IERNFLL+K+VLYFV+G++K+VQ LWL
Sbjct: 271 WELQLWKWESMVLVLICGRLVSSWIVKIVVFFIERNFLLRKRVLYFVYGVRKAVQNCLWL 330
Query: 254 SLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASFLIGAFLWLIKTLLLKILASKFH 313
LVL W LFD + + K L VT L+G LWL+KTLL+K+LAS FH
Sbjct: 331 GLVLLAWHFLFDEK----VAKAANTKALRVVTKIFVCLLVGFLLWLVKTLLVKVLASSFH 390
Query: 314 MNRFFDRIQESLFLHHVL-------------------------QTLLRPPLVEDESTAKF 373
M+ +FDRIQESLF +V+ + P VE +S A+
Sbjct: 391 MSTYFDRIQESLFTQYVIETLSGPPLIEIQKNEEEEERISVEVKKFQNPGGVEIQSGAQK 450
Query: 374 RCCRFCFESKKPDRKKVIDMG-------------KIHELKREKVSAWTMKVLVDAVTSSE 433
+ K P V+ G +H+L + VSAW MK L++ + +
Sbjct: 451 SPMK---TGKSPFLSHVLSNGGGGGGENKGITIDSLHKLNPKNVSAWKMKRLMNIIRNGS 510
Query: 434 MS-VSQILDDESYRDVADGDITNEMKVAKEAAKEIFKNVALPGNKFIEERDLLEFMIPEE 493
++ + + L D S D I +E + AK AA++IF NVA PG+KFI D++ F+ +E
Sbjct: 511 LTTLDEQLQDPSLDDDKGNQIRSEFE-AKLAARKIFHNVAKPGSKFIYANDIMRFLPDDE 570
Query: 494 VNLVWPHFE-VDKTRKIDMKALTNWVVKVYQGRKTLAHALKDTKTAVKQLNNLITALIIV 553
FE +T +I +L NWVV ++ R+ LA L DTKTAV +L+ ++ ++ +
Sbjct: 571 ALKTLSLFEGASETNRISKSSLKNWVVNAFRERRALALTLNDTKTAVNRLHKMVNIVVGI 630
Query: 554 VTAVIWLLLMEIATTKVLVFLLTQLAVAAFMFGNTCKTAFEALIFVFVMHPFDVGDRCVV 613
+ VIWL+++ I +TK LV + +Q+ V AF+FGN CK FE++I++FV+HPFDVGDRC +
Sbjct: 631 IILVIWLIILGITSTKFLVVMSSQVVVVAFIFGNMCKIVFESIIYLFVIHPFDVGDRCEI 690
Query: 614 DGVQLLVEEMNILTTVFLKLNNEKVYYPNSVLATKPITNYYRSPDMGDTVEFSIGFETPV 673
DGVQ++VEEMNILTTVFL+ +N+KV YPNS+L TK I NYYRSPDMGD +EFSI TP
Sbjct: 691 DGVQMVVEEMNILTTVFLRFDNQKVVYPNSLLWTKSIGNYYRSPDMGDGIEFSIHITTPA 750
Query: 674 ERIGAMKEQIKRYLEENPQHWYPNHNVVVKEIENVNKIKIALYTNHTMNFQDWAEKNQRR 716
E+I +K++I Y+E HWYP +V K++E++N ++IA++ H MN QD EK RR
Sbjct: 751 EKIILIKQRITSYIEGKKDHWYPAPMIVFKDMESLNSVRIAVWPTHRMNHQDMGEKWARR 810
BLAST of Cucsa.299750 vs. Swiss-Prot
Match:
MSL8_ARATH (Mechanosensitive ion channel protein 8 OS=Arabidopsis thaliana GN=MSL8 PE=2 SV=2)
HSP 1 Score: 464.5 bits (1194), Expect = 2.1e-129
Identity = 247/614 (40.23%), Postives = 379/614 (61.73%), Query Frame = 1
Query: 139 PEGEDDGNMGSNAKHKKVKVKTVIKWIGVFCIISCLVASLTVNPLKNRFLWGLKVWKWCL 198
P E+D + K K+ T+++W+ + II+ L SL++ K +W L +WKW +
Sbjct: 281 PLAEED--VPDEYKRGKLDAITLLQWLSLVAIIAALACSLSIQSWKKVRVWNLHLWKWEV 340
Query: 199 LATVILCGLIFTRWVMNVVVFLIERNFLLKKKVLYFVHGLKKSVQVTLWLSLVLATWGSL 258
V++CG + + W + +VVF IERNFLL+K+VLYFV+G++++VQ LWL LVL W L
Sbjct: 341 FLLVLICGRLVSGWGIRIVVFFIERNFLLRKRVLYFVYGVRRAVQNCLWLGLVLLAWHFL 400
Query: 259 FDRRNHMISSSRITAKILDAVTWTLASFLIGAFLWLIKTLLLKILASKFHMNRFFDRIQE 318
FD++ + SR L VT L FL+ LWLIKTL++K+LAS FH++ +FDRIQE
Sbjct: 401 FDKKVQRETRSRF----LPYVTKILVCFLLSTILWLIKTLVVKVLASSFHVSTYFDRIQE 460
Query: 319 SLFLHHVLQTLLRPPLVE------DESTAKFRC--------------CRFCFESKKPDR- 378
+LF +V++TL PP++E +E A+ C F K R
Sbjct: 461 ALFNQYVIETLSGPPMIEMSRIEEEEERAQDEIFKMQNAGANLPPDLCAAAFPPGKSGRV 520
Query: 379 ----------KKVIDMG----KIHELKREKVSAWTMKVLVDAVTSSEMS-VSQILDDESY 438
K D G +H + + +SAW MK L+ V + ++ + + + + +Y
Sbjct: 521 MNPKLSPIIPKSTTDNGISMEHLHRMNHKNISAWNMKRLMKIVRNVSLTTLDEQMLESTY 580
Query: 439 RDVADGDITNEMKVAKEAAKEIFKNVALPGNKFIEERDLLEFMIPEEVNLVWPHFE-VDK 498
D + I +E K AK AA++IFKNV G K+I DL+ F+ +E FE +
Sbjct: 581 EDESTRQIRSE-KEAKAAARKIFKNVEQRGAKYIYLEDLMRFLREDEAMKTMGLFEGAPE 640
Query: 499 TRKIDMKALTNWVVKVYQGRKTLAHALKDTKTAVKQLNNLITALIIVVTAVIWLLLMEIA 558
++I AL NW+V ++ R+ LA L DTKTAV +L+++I + +V VIWL+L+EIA
Sbjct: 641 NKRISKSALKNWLVNAFRERRALALTLNDTKTAVNKLHHMINIVTAIVIVVIWLVLLEIA 700
Query: 559 TTKVLVFLLTQLAVAAFMFGNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNIL 618
++KVL+F+ +Q+ + AF+FGNT KT FE++IF+F++HP+DVGDRC +D VQL+VEEMNIL
Sbjct: 701 SSKVLLFVSSQVVLLAFIFGNTVKTVFESIIFLFIVHPYDVGDRCEIDSVQLVVEEMNIL 760
Query: 619 TTVFLKLNNEKVYYPNSVLATKPITNYYRSPDMGDTVEFSIGFETPVERIGAMKEQIKRY 678
TTVFL+ +N K+ YPNS+L K I NYYRSPDMGD +EF + TP+E+I +K++I Y
Sbjct: 761 TTVFLRYDNLKIMYPNSLLWQKSINNYYRSPDMGDAIEFCVHITTPLEKISVIKQRISNY 820
Query: 679 LEENPQHWYPNHNVVVKEIENVNKIKIALYTNHTMNFQDWAEKNQRRTKLVMELKRIFEE 716
++ P++WYP ++VK++E+++ +++A++ H +N QD AE+ RR LV E+ +I E
Sbjct: 821 IDNKPEYWYPQAKIIVKDLEDLHIVRLAIWPCHRINHQDMAERWTRRAVLVEEVIKILLE 880
BLAST of Cucsa.299750 vs. TrEMBL
Match:
A0A0A0L5E6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G647440 PE=4 SV=1)
HSP 1 Score: 1440.6 bits (3728), Expect = 0.0e+00
Identity = 720/721 (99.86%), Postives = 721/721 (100.00%), Query Frame = 1
Query: 1 MDVSGNKGIRRGSSQKESGNGGEVVVQLSGVENECSVPKQNRADSQTKEPTGSSVGYDNA 60
MDVSGNKGIRRGSSQKESGNGGEVVVQLSGVENECSVPKQNRADSQTKEPTGSSVGYDNA
Sbjct: 1 MDVSGNKGIRRGSSQKESGNGGEVVVQLSGVENECSVPKQNRADSQTKEPTGSSVGYDNA 60
Query: 61 SPLAPTPNKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQSCFIDSDMLEEENHVSLRE 120
SPLAPTPNKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQSCFIDSDMLEEENHVSLRE
Sbjct: 61 SPLAPTPNKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQSCFIDSDMLEEENHVSLRE 120
Query: 121 QIGATSSRSSNLNTPKAQPEGEDDGNMGSNAKHKKVKVKTVIKWIGVFCIISCLVASLTV 180
QIGATSSRSSNLNTPKAQPEGEDDGNMGSNAKHKKVKVKTVIKWIGVFCIISCLVASLTV
Sbjct: 121 QIGATSSRSSNLNTPKAQPEGEDDGNMGSNAKHKKVKVKTVIKWIGVFCIISCLVASLTV 180
Query: 181 NPLKNRFLWGLKVWKWCLLATVILCGLIFTRWVMNVVVFLIERNFLLKKKVLYFVHGLKK 240
NPLKNRFLWGLKVWKWCLLATVILCGLIFTRWVMNVVVFLIERNFLLKKKVLYFVHGLKK
Sbjct: 181 NPLKNRFLWGLKVWKWCLLATVILCGLIFTRWVMNVVVFLIERNFLLKKKVLYFVHGLKK 240
Query: 241 SVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASFLIGAFLWLIKTLLL 300
SVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASFLIGAFLWLIKTLLL
Sbjct: 241 SVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASFLIGAFLWLIKTLLL 300
Query: 301 KILASKFHMNRFFDRIQESLFLHHVLQTLLRPPLVEDESTAKFRCCRFCFESKKPDRKKV 360
KILASKFHMNRFFDRIQESLFLHHVLQTLLRPPLVEDESTAKFRCCRFCFESKKPDRKKV
Sbjct: 301 KILASKFHMNRFFDRIQESLFLHHVLQTLLRPPLVEDESTAKFRCCRFCFESKKPDRKKV 360
Query: 361 IDMGKIHELKREKVSAWTMKVLVDAVTSSEMSVSQILDDESYRDVADGDITNEMKVAKEA 420
IDMGKIHELKREKVSAWTMKVLVDAVTSSEMSVSQILDDESYRDVADGDITNEMKVAKEA
Sbjct: 361 IDMGKIHELKREKVSAWTMKVLVDAVTSSEMSVSQILDDESYRDVADGDITNEMKVAKEA 420
Query: 421 AKEIFKNVALPGNKFIEERDLLEFMIPEEVNLVWPHFEVDKTRKIDMKALTNWVVKVYQG 480
AKEIFKNVALPGNKFIEERDLLEFMIPEEVNLVWPHFEVDKTRKIDMKALTNWVVKVYQG
Sbjct: 421 AKEIFKNVALPGNKFIEERDLLEFMIPEEVNLVWPHFEVDKTRKIDMKALTNWVVKVYQG 480
Query: 481 RKTLAHALKDTKTAVKQLNNLITALIIVVTAVIWLLLMEIATTKVLVFLLTQLAVAAFMF 540
RKTLAHALKDTKTAVKQLNNLITALIIVVTAVIWLLLMEIATTKVLVFLLTQLAVAAFMF
Sbjct: 481 RKTLAHALKDTKTAVKQLNNLITALIIVVTAVIWLLLMEIATTKVLVFLLTQLAVAAFMF 540
Query: 541 GNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEKVYYPNSVL 600
GNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEKVYYPNSVL
Sbjct: 541 GNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEKVYYPNSVL 600
Query: 601 ATKPITNYYRSPDMGDTVEFSIGFETPVERIGAMKEQIKRYLEENPQHWYPNHNVVVKEI 660
ATKPITNYYRSPDMGDTVEFSIGFETPVERIGAMKEQIKRYLEENPQHWYPNHNVVVKEI
Sbjct: 601 ATKPITNYYRSPDMGDTVEFSIGFETPVERIGAMKEQIKRYLEENPQHWYPNHNVVVKEI 660
Query: 661 ENVNKIKIALYTNHTMNFQDWAEKNQRRTKLVMELKRIFEELKINYNLLPQTVHLFPVEG 720
ENVNKIKIALYTNHTMNFQDWAEKN+RRTKLVMELKRIFEELKINYNLLPQTVHLFPVEG
Sbjct: 661 ENVNKIKIALYTNHTMNFQDWAEKNRRRTKLVMELKRIFEELKINYNLLPQTVHLFPVEG 720
Query: 721 H 722
H
Sbjct: 721 H 721
BLAST of Cucsa.299750 vs. TrEMBL
Match:
A0A067KQE3_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_07615 PE=4 SV=1)
HSP 1 Score: 812.0 bits (2096), Expect = 5.9e-232
Identity = 438/780 (56.15%), Postives = 551/780 (70.64%), Query Frame = 1
Query: 5 GNKGIRRGSSQKESGNGGEVVVQLSGVE--NECSVPK--------------QNRADSQTK 64
G KG +K+S NGGEV++ +S E N + PK Q+R +S +
Sbjct: 7 GRKGGNISMLEKKSTNGGEVLITISNEEKPNANATPKGLTSPKDQEILGSRQSRPNSPVQ 66
Query: 65 EPTGSSVGYDNASPLA----------PTPNKPPKIPISNGTLTPRRSLRRSTLSKPKSRF 124
E ++ G+ + P++ P+PNKPPK+P SN T RRSL RS SKPKSRF
Sbjct: 67 E--SNAFGFIKSMPISYPSPEISRFSPSPNKPPKVPASNENFTRRRSLTRSVYSKPKSRF 126
Query: 125 GEQSCFIDSDMLEEENHVSLREQIGATSSRSSNLN-----------------TPK----A 184
GEQ ID M EE++ ++L+EQ S ++L+ TPK A
Sbjct: 127 GEQPYPIDDAMFEEDS-LTLQEQFSRNSPYRNSLSRGSPNNKSASTIRTNSITPKTPLMA 186
Query: 185 QPEG--EDDGNMG--------SNAKHKKVKVKTVIKWIGVFCIISCLVASLTVNPLKNRF 244
P G +D+ + S KHK+++VK VI+W+ CI+ CLVA LTV L+
Sbjct: 187 SPGGPADDEDELDEVVKKVELSQEKHKRIRVKLVIQWVAFVCIVGCLVAGLTVEKLEKTM 246
Query: 245 LWGLKVWKWCLLATVILCGLIFTRWVMNVVVFLIERNFLLKKKVLYFVHGLKKSVQVTLW 304
+WGL WKW +L V G+ T W M+ +VFLIERNFLL+KKVLYFV+GLKKSVQV +W
Sbjct: 247 IWGLDFWKWSVLVMVTFSGMSITNWFMHFIVFLIERNFLLRKKVLYFVYGLKKSVQVFMW 306
Query: 305 LSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASFLIGAFLWLIKTLLLKILASKF 364
+ LVL W LF+R + S+ KIL+ VTWTL S LIG+FLWL+KTLLLKILAS F
Sbjct: 307 IGLVLLAWAFLFNRG---VERSKTATKILNYVTWTLISLLIGSFLWLLKTLLLKILASNF 366
Query: 365 HMNRFFDRIQESLFLHHVLQTLLRPPLVEDESTA---------KFRCCRFCFESKKPDRK 424
H+ +FFDRIQES+F +VLQTL PPL+E+ FR + + K+ K
Sbjct: 367 HVTKFFDRIQESVFHQYVLQTLSGPPLIEEAERVGRSASTGQLSFRSTK---KGKETKEK 426
Query: 425 KVIDMGKIHELKREKVSAWTMKVLVDAVTSSEMS-VSQILDDE--SYRDVADGDITNEMK 484
KVIDMG +H++K+EKVSAWTMKVLVDA+T+S +S +S LD+ + D +ITNEM+
Sbjct: 427 KVIDMGMLHKIKQEKVSAWTMKVLVDAITNSGLSTISNTLDESIGGAGEQTDSEITNEME 486
Query: 485 VAKEAAKEIFKNVALPGNKFIEERDLLEFMIPEEVNLVWPHFEVDKTRKIDMKALTNWVV 544
A AA IF+NVA PG K+I+E DLL FMI EEV+LV+P FEV +TR+I+ KALT+WVV
Sbjct: 487 -ATAAAYHIFRNVAKPGWKYIDEEDLLRFMIKEEVDLVFPLFEVSETRQIERKALTDWVV 546
Query: 545 KVYQGRKTLAHALKDTKTAVKQLNNLITALIIVVTAVIWLLLMEIATTKVLVFLLTQLAV 604
KVY+GRK LAHAL DTKTAVKQLN L+T +++VVT +IWLLLM IATTKV+V L +QL V
Sbjct: 547 KVYKGRKALAHALSDTKTAVKQLNKLVTVILVVVTVIIWLLLMGIATTKVIVVLSSQLVV 606
Query: 605 AAFMFGNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEKVYY 664
AAF+FGNTCKT FEA+IFVFVMHPFDVGDRCVVDGV LLVEEMNILTTVFLKL+NEK+YY
Sbjct: 607 AAFIFGNTCKTMFEAIIFVFVMHPFDVGDRCVVDGVPLLVEEMNILTTVFLKLDNEKIYY 666
Query: 665 PNSVLATKPITNYYRSPDMGDTVEFSIGFETPVERIGAMKEQIKRYLEENPQHWYPNHNV 716
PNSVLATKPI+NYYRSPDMGD+VEFSI F TP E+IG +K++IK++LE+NPQHW+PNH+V
Sbjct: 667 PNSVLATKPISNYYRSPDMGDSVEFSIDFSTPTEKIGLLKDKIKKFLEKNPQHWHPNHSV 726
BLAST of Cucsa.299750 vs. TrEMBL
Match:
A0A059CMY1_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_C00709 PE=4 SV=1)
HSP 1 Score: 789.6 bits (2038), Expect = 3.1e-225
Identity = 435/780 (55.77%), Postives = 534/780 (68.46%), Query Frame = 1
Query: 5 GNKGIRRGSSQKESGNGGEVVVQLS------------------GVENECSVPKQNRADSQ 64
G KG SQK + +G +VVV +S E E + PKQ DS
Sbjct: 7 GLKGGEIAMSQKRNVSGNDVVVNISTEGDAKAVTEGGGLKGSSSKELETAAPKQTPVDSA 66
Query: 65 TKEPTGSSVGYDNASP----------LAPTPNKPPKIPISNGTLTPRRSLRRSTLSKPKS 124
+ + SS + P L+P+ NKPPK P +N +L +RSL RS SKPKS
Sbjct: 67 CQASSESSTVVAKSVPMSCPSPEIAGLSPSRNKPPKAPNANESLVRKRSLNRSIFSKPKS 126
Query: 125 RFGEQSCFIDSDMLEEENHVSLREQIGATSSRSSNLN-----------------TPKAQP 184
RFGEQ +D+ M EEE + L+EQ G S + N TP+
Sbjct: 127 RFGEQQYPLDATMFEEEPNALLQEQAGGHSPFRGSFNQASPNNRSTRSAGNVPVTPRPVG 186
Query: 185 EG---EDDGNMG-------SNAKHKKVKVKTVIKWIGVFCIISCLVASLTVNPLKNRFLW 244
G EDD + S AK K++K K +++W F I+ CLVAS TV+ L+ LW
Sbjct: 187 GGGGEEDDEEIYKQVTLELSKAKQKRIKTKALVEWTLFFLILGCLVASSTVDQLEACKLW 246
Query: 245 GLKVWKWCLLATVILCGLIFTRWVMNVVVFLIERNFLLKKKVLYFVHGLKKSVQVTLWLS 304
GL++WKWC+L V+ G++ T WVM+ +VFLIE+ FLL+KKVLYFVHGLKKSVQV WL+
Sbjct: 247 GLEMWKWCVLVMVLFSGMLVTNWVMHFIVFLIEKKFLLRKKVLYFVHGLKKSVQVFTWLA 306
Query: 305 LVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASFLIGAFLWLIKTLLLKILASKFHM 364
VL TW LF+R + S+I K+LD +TWTL S LIG+FLW +KTL+LKILAS FH+
Sbjct: 307 FVLLTWVLLFNRG---VRRSKIANKVLDCITWTLVSILIGSFLWFLKTLMLKILASSFHV 366
Query: 365 NRFFDRIQESLFLHHVLQTLLRPPLVED-ESTAKFRCCR-FCFESKKPDR----KKVIDM 424
FFDRIQES+F H+VLQTL PPL+E+ E K R F F + K + KKVIDM
Sbjct: 367 TAFFDRIQESIFHHYVLQTLSGPPLIEEAERVGKDPSVREFSFRTMKKGKLGKEKKVIDM 426
Query: 425 GKIHELKREKVSAWTMKVLVDAVTSSEMSVSQILDDESYRD----VADGDITNEMKVAKE 484
GKI+ +K+EKVSAWTMK+L+DAVT+S +S DES D AD +ITNEM+ A
Sbjct: 427 GKIYRMKQEKVSAWTMKMLIDAVTNSGLSTLSNTLDESVDDGGVEQADKEITNEME-AMA 486
Query: 485 AAKEIFKNVALPGNKFIEERDLLEFMIPEEVNLVWPHFEVDKTRKIDMKALTNWVVKVYQ 544
AA IF+NVA P K+ EE DL FMI EEV+L++P E T KID+K+L++WVVKVYQ
Sbjct: 487 AAYHIFRNVAPPSRKYFEEEDLGRFMIKEEVDLIFPLLEGAATGKIDIKSLSDWVVKVYQ 546
Query: 545 GRKTLAHALKDTKTAVKQLNNLITALIIVVTAVIWLLLMEIATTKVLVFLLTQLAVAAFM 604
GRK L HAL DTKTAVKQLN L+T ++IV+T +IWLLLMEIATTKVLV L +QL VAAF+
Sbjct: 547 GRKALVHALTDTKTAVKQLNKLVTGILIVITIIIWLLLMEIATTKVLVLLSSQLVVAAFI 606
Query: 605 FGNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEKVYYPNSV 664
FGNTCKT FEA+IFVFVMHPFDVGDRCV+DGVQLLVEEMNIL TVFLKL+NEK+YYPNSV
Sbjct: 607 FGNTCKTIFEAIIFVFVMHPFDVGDRCVIDGVQLLVEEMNILNTVFLKLDNEKIYYPNSV 666
Query: 665 LATKPITNYYRSPDMGDTVEFSIGFETPVERIGAMKEQIKRYLEENPQHWYPNHNVVVKE 720
L+TKPI+NY RS DMGD VEFSI TP+E IGAMKE+IK+YLE+N HW PNHN++V E
Sbjct: 667 LSTKPISNYNRSSDMGDKVEFSIDIMTPLETIGAMKEKIKKYLEKNQLHWRPNHNLLVSE 726
BLAST of Cucsa.299750 vs. TrEMBL
Match:
B9RQV1_RICCO (Putative uncharacterized protein OS=Ricinus communis GN=RCOM_0706730 PE=4 SV=1)
HSP 1 Score: 788.1 bits (2034), Expect = 9.1e-225
Identity = 423/753 (56.18%), Postives = 527/753 (69.99%), Query Frame = 1
Query: 14 SQKESGNGG--EVVVQLSGVE---------NECSVPKQNRADSQTKEPTGSS----VGYD 73
++K++ NGG EV++ +SG E ++ KQ+R S KE G + V +
Sbjct: 2 AEKKTSNGGRGEVLITISGDETPKGSASKDSDSVASKQSRPSSPVKESNGGAFAKTVSIN 61
Query: 74 NASP----LAPTPNKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQSCFIDSDMLEEEN 133
N SP L PTP KPPKIP+SN LT RRSL RS SKPKSRFGEQ +D+ +LEE++
Sbjct: 62 NHSPEISSLNPTPTKPPKIPVSNENLTRRRSLARSVYSKPKSRFGEQPVLVDATVLEEDS 121
Query: 134 HVSLREQIG-------------ATSSRSSNLNTPKAQPEGEDDGN----------MGSNA 193
+ L EQI A + +S++ T P G D
Sbjct: 122 LI-LEEQISRNLSYRKSLSRGSANNKSASSIRTNSMNPNGPVDDEDEVEDVVKKVQSIKE 181
Query: 194 KHKKVKVKTVIKWIGVFCIISCLVASLTVNPLKNRFLWGLKVWKWCLLATVILCGLIFTR 253
K+K+V K VI+WI C+ CLVASLTV L+ +WGL+ WKWC+L VI+ G+ T
Sbjct: 182 KNKRVGAKAVIQWITFVCLAGCLVASLTVQKLEKTMIWGLEPWKWCVLLLVIISGMFITN 241
Query: 254 WVMNVVVFLIERNFLLKKKVLYFVHGLKKSVQVTLWLSLVLATWGSLFDRRNHMISSSRI 313
W M+ +VF+IERNFLL+KKVLYFV+GLK SVQV +W+ LVL W L D H I S+
Sbjct: 242 WFMHFIVFVIERNFLLRKKVLYFVYGLKNSVQVFVWIGLVLLAWAFLID---HEIGRSKT 301
Query: 314 TAKILDAVTWTLASFLIGAFLWLIKTLLLKILASKFHMNRFFDRIQESLFLHHVLQTLLR 373
IL VTWTL S LIG+FLWL+K L LKILAS FH+N+FFDRIQES+F +VLQTL
Sbjct: 302 ATTILKCVTWTLMSLLIGSFLWLVKNLSLKILASNFHVNKFFDRIQESVFNQYVLQTLSG 361
Query: 374 PPLVED-----ESTAKFRCCRFCFESKKPDRKKVIDMGKIHELKREKVSAWTMKVLVDAV 433
PPL+E+ ST+ + ++ K + KKVID+G +H++K+EKVSAWTMKVLVDAV
Sbjct: 362 PPLIEEAERVGRSTSSGQLSFRSTKNGKTEEKKVIDIGMLHKVKQEKVSAWTMKVLVDAV 421
Query: 434 TSSEMSVSQILDDESY----RDVADGDITNEMKVAKEAAKEIFKNVALPGNKFIEERDLL 493
TSS +S +ES + D +ITNEM+ A AA IF+NVA PG K+I+E DLL
Sbjct: 422 TSSGLSTLSNTLEESVGGRDKQTTDMEITNEME-ATAAAYHIFRNVAKPGWKYIDEDDLL 481
Query: 494 EFMIPEEVNLVWPHFEVDKTRKIDMKALTNWVVKVYQGRKTLAHALKDTKTAVKQLNNLI 553
FMI EEV+LV P FE + +ID K+LT+WVVKVY+ RK LAHAL DTKTAVKQLN L+
Sbjct: 482 RFMIKEEVDLVLPLFEASENGQIDRKSLTDWVVKVYKDRKALAHALGDTKTAVKQLNKLV 541
Query: 554 TALIIVVTAVIWLLLMEIATTKVLVFLLTQLAVAAFMFGNTCKTAFEALIFVFVMHPFDV 613
T ++I+VT VIWLLL+E+ATTKVL+ LL+Q VAAFM NTCKT FEAL+FVFVMHPFDV
Sbjct: 542 TGILIIVTIVIWLLLIEVATTKVLMVLLSQFLVAAFMAKNTCKTVFEALMFVFVMHPFDV 601
Query: 614 GDRCVVDGVQLLVEEMNILTTVFLKLNNEKVYYPNSVLATKPITNYYRSPDMGDTVEFSI 673
GDRCVVDGV LLVEEMNILTTVFLKL+NEK+YYPNSVLA KPI+NYYRSPDMGD VEFSI
Sbjct: 602 GDRCVVDGVALLVEEMNILTTVFLKLDNEKIYYPNSVLANKPISNYYRSPDMGDAVEFSI 661
Query: 674 GFETPVERIGAMKEQIKRYLEENPQHWYPNHNVVVKEIENVNKIKIALYTNHTMNFQDWA 716
F TP E+IG +K++IK+YLE PQ+WYP H VVKEIENVN++K+ALY NH MNFQ++
Sbjct: 662 DFATPSEKIGLLKDKIKQYLENTPQYWYPGHGFVVKEIENVNRLKLALYCNHKMNFQEFG 721
BLAST of Cucsa.299750 vs. TrEMBL
Match:
W9R6V9_9ROSA (Mechanosensitive ion channel protein 10 OS=Morus notabilis GN=L484_018576 PE=4 SV=1)
HSP 1 Score: 780.0 bits (2013), Expect = 2.5e-222
Identity = 429/753 (56.97%), Postives = 533/753 (70.78%), Query Frame = 1
Query: 4 SGNKGIRRGSSQKESGNGGEVVVQLSGVE------NECSVPKQ-NRADSQTK---EPTGS 63
+ + G S+K++ NG VVV++ +E PKQ + DS K E TG
Sbjct: 7 TSSNGGETSMSEKKTENGSAVVVEIRTESESAKKGSESPFPKQLTKTDSPQKVSTESTGI 66
Query: 64 SVGYDNASP------LAPTPNKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQSCFIDS 123
S + P +P+PNKPPKIP + TLT R+SL RS S+PKSRFGE S IDS
Sbjct: 67 SKSVPVSCPSPEIGRFSPSPNKPPKIPATYDTLTRRKSLNRSVFSRPKSRFGEPSVPIDS 126
Query: 124 DMLEEEN---HVSLREQIGATSSRSS--NLNTPK----AQPEGEDDGN-------MGSNA 183
M EE N + L + +R+S N++T + AQ +D + + S
Sbjct: 127 AMFEEHNGSGQIGLGSPYRGSFNRASPNNVSTARTVSIAQKSAAEDEDEEIYKKVVLSEE 186
Query: 184 KHKKVKVKTVIKWIGVFCIISCLVASLTVNPLKNRFLWGLKVWKWCLLATVILCGLIFTR 243
K K+VK K +++WI ++ CL ASLTV L++ LW L++WKW +L VI+CG++ T
Sbjct: 187 KRKRVKAKVLVEWIMFLFLVVCLTASLTVEGLESTKLWELELWKWVVLVMVIICGMLVTN 246
Query: 244 WVMNVVVFLIERNFLLKKKVLYFVHGLKKSVQVTLWLSLVLATWGSLFDRRNHMISSSRI 303
W M +VVF+IERNFLL+KKVLYFVHGLKKSVQV +WL LVL TW +F NH +
Sbjct: 247 WFMRIVVFVIERNFLLRKKVLYFVHGLKKSVQVFIWLCLVLLTWVLVF---NHGVKRPET 306
Query: 304 TAKILDAVTWTLASFLIGAFLWLIKTLLLKILASKFHMNRFFDRIQESLFLHHVLQTLLR 363
KIL VTWTL + LIG F+WLIKTLLLKILAS FH+N FFDRIQES+F +VLQTL
Sbjct: 307 ANKILHYVTWTLVTLLIGGFIWLIKTLLLKILASNFHVNTFFDRIQESIFHQYVLQTLSG 366
Query: 364 PPLVED-ESTAKFRCC-RFCFESKKPDRK----KVIDMGKIHELKREKVSAWTMKVLVDA 423
P L+E+ E + + F S K + + IDM +H++K+EKVSAWTMKVLVDA
Sbjct: 367 PALIEEAEKVGRSPSMGQLSFRSTKKGKAAKTIETIDMANLHKMKQEKVSAWTMKVLVDA 426
Query: 424 VTSSEMS-VSQILDD--ESYRDVADGDITNEMKVAKEAAKEIFKNVALPGNKFIEERDLL 483
V+SS +S +S LD+ + D +IT+EM+ A AA IF+NVA PG+KFI+E DLL
Sbjct: 427 VSSSGLSTISNTLDEMENGAMEQMDKEITSEME-ATAAAYHIFRNVAQPGSKFIDEDDLL 486
Query: 484 EFMIPEEVNLVWPHFEVDKTRKIDMKALTNWVVKVYQGRKTLAHALKDTKTAVKQLNNLI 543
FMI EEV++V P F D R ID KALT+WV+KVY GRK LAHAL DTKTAVKQLN L+
Sbjct: 487 RFMIKEEVDIVLPLFATDNGR-IDRKALTDWVIKVYNGRKALAHALNDTKTAVKQLNKLV 546
Query: 544 TALIIVVTAVIWLLLMEIATTKVLVFLLTQLAVAAFMFGNTCKTAFEALIFVFVMHPFDV 603
T ++ VV ++WLLLMEIATTKVLVFL +QL VAAFMFGNTCKT FEA++FVFVMHPFDV
Sbjct: 547 TGILFVVIILVWLLLMEIATTKVLVFLSSQLVVAAFMFGNTCKTIFEAIVFVFVMHPFDV 606
Query: 604 GDRCVVDGVQLLVEEMNILTTVFLKLNNEKVYYPNSVLATKPITNYYRSPDMGDTVEFSI 663
GDRCV+DGV LLVEEMNIL TVFLKLNNEKVYYPNSVL+TKPI+NYYRS DMGDTVEFSI
Sbjct: 607 GDRCVIDGVPLLVEEMNILNTVFLKLNNEKVYYPNSVLSTKPISNYYRSSDMGDTVEFSI 666
Query: 664 GFETPVERIGAMKEQIKRYLEENPQHWYPNHNVVVKEIENVNKIKIALYTNHTMNFQDWA 716
F TPVE+IG +K++I +Y+E+NPQ W+PNH+VVV EIENVNK+K+ALY NHT++FQ++
Sbjct: 667 DFMTPVEKIGHLKDKINKYIEKNPQQWHPNHSVVVVEIENVNKLKMALYVNHTISFQEYG 726
BLAST of Cucsa.299750 vs. TAIR10
Match:
AT5G12080.1 (AT5G12080.1 mechanosensitive channel of small conductance-like 10)
HSP 1 Score: 706.4 bits (1822), Expect = 1.8e-203
Identity = 387/740 (52.30%), Postives = 504/740 (68.11%), Query Frame = 1
Query: 14 SQKESGNGGEVVVQLSGVENECSVPKQNRADSQTKEPTGSSVGYDNASPLAPTPNKPPKI 73
S G GG+VV+ + VE K+ + K S S L +PNKPP+
Sbjct: 6 SSNGGGGGGDVVINVP-VEEASRRSKEMASPESEKGVPFSKSPSPEISKLVGSPNKPPRA 65
Query: 74 PISNGT-LTPRRSLRRSTLSKPKSRFGEQSCFIDSDMLEEENHVSLREQIGATSSRSSNL 133
P N LT R+S RS SKPKSRF + SC +D+ +LEEE +REQ+GA S S
Sbjct: 66 PNQNNVGLTQRKSFARSVYSKPKSRFVDPSCPVDTSILEEE----VREQLGAGFSFS--- 125
Query: 134 NTPKAQPEGEDDGNMGSNAK----------------HKKVKVK----------TVIKWIG 193
+A P + + ++GS A +KKVK+ +I+
Sbjct: 126 ---RASPNNKSNRSVGSPAPVTPSKVVVEKDEDEEIYKKVKLNREMRSKISTLALIESAF 185
Query: 194 VFCIISCLVASLTVNPLKNRFLWGLKVWKWCLLATVILCGLIFTRWVMNVVVFLIERNFL 253
I+S LVASLT+N LK+ WGL+VWKWC+L VI G++ T W M ++VFLIE NFL
Sbjct: 186 FVVILSALVASLTINVLKHHTFWGLEVWKWCVLVMVIFSGMLVTNWFMRLIVFLIETNFL 245
Query: 254 LKKKVLYFVHGLKKSVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASF 313
L++KVLYFVHGLKKSVQV +WL L+L W LF NH + S K+L +T TL S
Sbjct: 246 LRRKVLYFVHGLKKSVQVFIWLCLILVAWILLF---NHDVKRSPAATKVLKCITRTLISI 305
Query: 314 LIGAFLWLIKTLLLKILASKFHMNRFFDRIQESLFLHHVLQTLLRPPLVED------EST 373
L GAF WL+KTLLLKILA+ F++N FFDRIQ+S+F +VLQTL PL+E+ E +
Sbjct: 306 LTGAFFWLVKTLLLKILAANFNVNNFFDRIQDSVFHQYVLQTLSGLPLMEEAERVGREPS 365
Query: 374 AKFRCCRFCFESKKPDRKKVIDMGKIHELKREKVSAWTMKVLVDAVTSSEMS-VSQILDD 433
+ KKVIDMGK+H++KREKVSAWTM+VL++AV +S +S +S LD+
Sbjct: 366 TGHLSFATVVKKGTVKEKKVIDMGKVHKMKREKVSAWTMRVLMEAVRTSGLSTISDTLDE 425
Query: 434 ESY---RDVADGDITNEMKVAKEAAKEIFKNVALPGNKFIEERDLLEFMIPEEVNLVWPH 493
+Y ++ AD +IT+EM+ A AA +F+NVA P +IEE DLL FMI EEV+LV+P
Sbjct: 426 TAYGEGKEQADREITSEME-ALAAAYHVFRNVAQPFFNYIEEEDLLRFMIKEEVDLVFPL 485
Query: 494 FE-VDKTRKIDMKALTNWVVKVYQGRKTLAHALKDTKTAVKQLNNLITALIIVVTAVIWL 553
F+ +T +I KA T WVVKVY R+ LAH+L DTKTAVKQLN L+TA+++VVT VIWL
Sbjct: 486 FDGAAETGRITRKAFTEWVVKVYTSRRALAHSLNDTKTAVKQLNKLVTAILMVVTVVIWL 545
Query: 554 LLMEIATTKVLVFLLTQLAVAAFMFGNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLV 613
LL+E+ATTKVL+F TQL AF+ G+TCK FE+++FVFVMHP+DVGDRCVVDGV +LV
Sbjct: 546 LLLEVATTKVLLFFSTQLVALAFIIGSTCKNLFESIVFVFVMHPYDVGDRCVVDGVAMLV 605
Query: 614 EEMNILTTVFLKLNNEKVYYPNSVLATKPITNYYRSPDMGDTVEFSIGFETPVERIGAMK 673
EEMN+LTTVFLKLNNEKVYYPN+VLATKPI+NY+RSP+MG+TVEFSI F TPV +I +K
Sbjct: 606 EEMNLLTTVFLKLNNEKVYYPNAVLATKPISNYFRSPNMGETVEFSISFSTPVSKIAHLK 665
Query: 674 EQIKRYLEENPQHWYPNHNVVVKEIENVNKIKIALYTNHTMNFQDWAEKNQRRTKLVMEL 716
E+I YLE+NPQHW P H+VVVKEIEN+NK+K+ALY++HT+ FQ+ E+N RRT+L + +
Sbjct: 666 ERIAEYLEQNPQHWAPVHSVVVKEIENMNKLKMALYSDHTITFQENRERNLRRTELSLAI 725
BLAST of Cucsa.299750 vs. TAIR10
Match:
AT5G19520.1 (AT5G19520.1 mechanosensitive channel of small conductance-like 9)
HSP 1 Score: 648.3 bits (1671), Expect = 5.7e-186
Identity = 356/748 (47.59%), Postives = 487/748 (65.11%), Query Frame = 1
Query: 14 SQKESGNGGEVVVQLSGVENECSVPKQNRADSQTKEPTGSSVGYDNASPLAP----TP-- 73
+++ NG EVV+ +S E+ P+ + + + P S G + + P+ P TP
Sbjct: 2 AERRVSNGEEVVINVSDKEDSKD-PRASPSFNPLASPD-SDAGIEKSKPVPPISIPTPEI 61
Query: 74 -------NKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQSCFIDSDMLEEENHVSLRE 133
+KPPKIP G L R+SL RS SKPKSRFGEQ F EE SLRE
Sbjct: 62 YKFSGSVHKPPKIPSPEG-LVRRKSLSRSIYSKPKSRFGEQQSFRYDSTREENGGRSLRE 121
Query: 134 QIGATSSRSSNLNTPKAQPEGEDDGNMGSNAK--------------HKKVKVKTV----- 193
Q GA S + + +A P + + ++ S A +KKVK+ V
Sbjct: 122 QFGAGSFARGSFD--RASPNNKSNRSVASAALSKVAEEEPDENEEIYKKVKLHRVKRSGM 181
Query: 194 -----IKWIGVFCIISCLVASLTVNPLKNRFLWGLKVWKWCLLATVILCGLIFTRWVMNV 253
++ + I+ L+ SLT++ + +WGL+ WKWC+L V L G++ T W M+
Sbjct: 182 KPLAFLELVVFMAILGALIVSLTIDVVNKHTIWGLEFWKWCVLVMVTLSGMLVTNWFMHF 241
Query: 254 VVFLIERNFLLKKKVLYFVHGLKKSVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKIL 313
VVF+IE+N+LL+KKVLYFVHGLKK+VQV +W SLVL W LFD + +R T + L
Sbjct: 242 VVFIIEKNYLLRKKVLYFVHGLKKNVQVFIWFSLVLIAWICLFDGD---VKRTRKTKRFL 301
Query: 314 DAVTWTLASFLIGAFLWLIKTLLLKILASKFHMNRFFDRIQESLFLHHVLQTLLRPPLVE 373
D +TWT+ S L+G+ L+L+KT LK+LASKF++ FF+RIQES+F +VLQTL PPL+E
Sbjct: 302 DFITWTIVSLLVGSILFLVKTFALKVLASKFNVRNFFERIQESVFHQYVLQTLSGPPLIE 361
Query: 374 DE-------STAKFRCCRFCFESKKPDRKKVIDMGKIHELKREKVSAWTMKVLVDAVTSS 433
+ ST R + K KKVIDMGK+H +K+EKVSAWTM+VL++AV +S
Sbjct: 362 EAENVGRVPSTGHLSFTRT--KDGKVKDKKVIDMGKVHRMKQEKVSAWTMRVLIEAVGTS 421
Query: 434 EMS-VSQILDD-ESYRDVADGDITNEMKVAKEAAKEIFKNVALPGNKFIEERDLLEFMIP 493
+S +S LD+ + ++ D +ITNEM+ A AA ++F NVA P + +IEE DLL FMI
Sbjct: 422 GISTISSTLDEVNNKKERTDKEITNEME-AVAAAYDVFNNVAKPNHNYIEEDDLLRFMIK 481
Query: 494 EEVNLVWPHFEVDKTRKIDMKALTNWVVKVYQGRKTLAHALKDTKTAVKQLNNLITALII 553
EEV+LV P E T KI K T WVV VY RKT+ H+L DTKTAVKQL+ LIT ++
Sbjct: 482 EEVDLVLPLIEDADTGKITRKTFTEWVVNVYTSRKTIGHSLNDTKTAVKQLDKLITGILT 541
Query: 554 VVTAVIWLLLMEIATTKVLVFLLTQLAVAAFMFGNTCKTAFEALIFVFVMHPFDVGDRCV 613
V+T ++W++L++IA+TK+L+ +Q AFM G+TCK FE+ +FVFVMHP+DVGDRCV
Sbjct: 542 VITFIVWMVLLDIASTKLLLVFSSQFLGLAFMIGSTCKNIFESFMFVFVMHPYDVGDRCV 601
Query: 614 VDGVQLLVEEMNILTTVFLKLNNEKVYYPNSVLATKPITNYYRSPDMGDTVEFSIGFETP 673
VDGV LLVEE+++LTTVFLK++NEKV+YPNSVL +KPI+N+YRSPDMGD V+F I F TP
Sbjct: 602 VDGVMLLVEEIDLLTTVFLKIDNEKVFYPNSVLISKPISNFYRSPDMGDYVDFGIAFSTP 661
Query: 674 VERIGAMKEQIKRYLEENPQHWYPNHNVVVKEIENVNKIKIALYTNHTMNFQDWAEKNQR 716
E+IG +K +I YL N QHWYP V+V+ IEN+NK+ + + HT+NFQ + EK+ R
Sbjct: 662 AEKIGCLKGKIGEYLVANSQHWYPEAQVMVRAIENMNKLVLNILVQHTINFQVYVEKSLR 721
BLAST of Cucsa.299750 vs. TAIR10
Match:
AT3G14810.1 (AT3G14810.1 mechanosensitive channel of small conductance-like 5)
HSP 1 Score: 473.0 bits (1216), Expect = 3.3e-133
Identity = 255/618 (41.26%), Postives = 375/618 (60.68%), Query Frame = 1
Query: 138 QPEGED---DGNMGSNAKHKKVKVKTVIKWIGVFCIISCLVASLTVNPLKNRFLWGLKVW 197
+ E ED D ++ K K+ ++WI + I++ LV SLT++ L+ + W L +W
Sbjct: 244 EEEEEDPFLDEDLPEEFKRDKLSFWVFLEWISLVLIVTSLVCSLTIHNLQRKTWWKLDLW 303
Query: 198 KWCLLATVILCGLIFTRWVMNVVVFLIERNFLLKKKVLYFVHGLKKSVQVTLWLSLVLAT 257
KW + V++CG + + W++ ++VFL+E+NF +K+VLYFV+G++KSVQ LWL LVL
Sbjct: 304 KWEVTVLVLICGRLVSSWIVRIIVFLVEKNFTWRKRVLYFVYGVRKSVQNCLWLGLVLLA 363
Query: 258 WGSLFDRRNHMISSSRITAKILDAVTWTLASFLIGAFLWLIKTLLLKILASKFHMNRFFD 317
W LFD++ + +R TA L VT L L+ +WL+KT+L+K+LAS FHM+ +FD
Sbjct: 364 WHFLFDKK--VERETRSTA--LRYVTRVLVCLLVALIIWLVKTILVKVLASSFHMSTYFD 423
Query: 318 RIQESLFLHHVLQTLLRPPLVEDES-------------------------TAKFRCCRFC 377
RIQESLF +V++TL PPL+E + K F
Sbjct: 424 RIQESLFTQYVIETLSGPPLMEIQRMEEEEQQVAEDVKSLEKLAGAKLPPALKATVKSFM 483
Query: 378 FESKKPDRKKV----------IDMGKIHELKREKVSAWTMKVLVDAVTSSEMS-VSQILD 437
K P ++ I + ++ + + VSAW MK L++ + +S + Q +
Sbjct: 484 KVGKSPGLNRIGSKRGEDGEGIRIDQLKRMNTKNVSAWNMKRLMNIILKGAISTLDQNMQ 543
Query: 438 DESYRDVADGDITNEMKVAKEAAKEIFKNVALPGNKFIEERDLLEFMIPEEVNLVWPHFE 497
D + D I +E + AK AA++IF NV PG+++I D L F+ EE FE
Sbjct: 544 DTTQEDEDATHIRSEYE-AKCAARKIFHNVTEPGSRYIYLEDFLRFLCEEEAERAMALFE 603
Query: 498 -VDKTRKIDMKALTNWVVKVYQGRKTLAHALKDTKTAVKQLNNLITALIIVVTAVIWLLL 557
++ KI L NWVVK ++ R+ LA L DTKTAV +L+ +I +I ++ +IWLL+
Sbjct: 604 GASESDKISKSCLKNWVVKAFRERRALALTLNDTKTAVDRLHRIINVVIGIIIIIIWLLI 663
Query: 558 MEIATTKVLVFLLTQLAVAAFMFGNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEE 617
+ IATT+ L+ L +QL + AF+FGN+CKT FEA+IF+FVMHPFDVGDRC +DGVQL+VEE
Sbjct: 664 LGIATTRFLLVLSSQLLLVAFVFGNSCKTIFEAIIFLFVMHPFDVGDRCEIDGVQLVVEE 723
Query: 618 MNILTTVFLKLNNEKVYYPNSVLATKPITNYYRSPDMGDTVEFSIGFETPVERIGAMKEQ 677
MNILTTVFL+ +N+K+ YPNSVL TKPI NYYRSPDMGD VEF + TP E+I A+K++
Sbjct: 724 MNILTTVFLRYDNQKIIYPNSVLGTKPIANYYRSPDMGDAVEFCVHIATPPEKITAIKQR 783
Query: 678 IKRYLEENPQHWYPNHNVVVKEIENVNKIKIALYTNHTMNFQDWAEKNQRRTKLVMELKR 716
I Y++ +WYP +V ++++N +KIA++ H MN QD E+ RR L+ E+ +
Sbjct: 784 ILSYVDNKKDYWYPAPMIVFLSMDDLNSVKIAVWLTHRMNHQDMGERYIRRGLLLEEVGK 843
BLAST of Cucsa.299750 vs. TAIR10
Match:
AT1G78610.1 (AT1G78610.1 mechanosensitive channel of small conductance-like 6)
HSP 1 Score: 471.9 bits (1213), Expect = 7.3e-133
Identity = 255/627 (40.67%), Postives = 378/627 (60.29%), Query Frame = 1
Query: 134 TPKAQPEGEDDG-----NMGSNAKHKKVKVKTVIKWIGVFCIISCLVASLTVNPLKNRFL 193
+PK Q E E+D ++ + K+ + V++W+ + II+ V +L + L+ + L
Sbjct: 211 SPKTQGEEEEDDPFAAEDLPEEYRKDKLSLWIVLEWLSLILIIAGFVCTLAIPSLRKKKL 270
Query: 194 WGLKVWKWCLLATVILCGLIFTRWVMNVVVFLIERNFLLKKKVLYFVHGLKKSVQVTLWL 253
W L++WKW + V++CG + + W++ +VVF IERNFLL+K+VLYFV+G++K+VQ LWL
Sbjct: 271 WELQLWKWESMVLVLICGRLVSSWIVKIVVFFIERNFLLRKRVLYFVYGVRKAVQNCLWL 330
Query: 254 SLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASFLIGAFLWLIKTLLLKILASKFH 313
LVL W LFD + + K L VT L+G LWL+KTLL+K+LAS FH
Sbjct: 331 GLVLLAWHFLFDEK----VAKAANTKALRVVTKIFVCLLVGFLLWLVKTLLVKVLASSFH 390
Query: 314 MNRFFDRIQESLFLHHVL-------------------------QTLLRPPLVEDESTAKF 373
M+ +FDRIQESLF +V+ + P VE +S A+
Sbjct: 391 MSTYFDRIQESLFTQYVIETLSGPPLIEIQKNEEEEERISVEVKKFQNPGGVEIQSGAQK 450
Query: 374 RCCRFCFESKKPDRKKVIDMG-------------KIHELKREKVSAWTMKVLVDAVTSSE 433
+ K P V+ G +H+L + VSAW MK L++ + +
Sbjct: 451 SPMK---TGKSPFLSHVLSNGGGGGGENKGITIDSLHKLNPKNVSAWKMKRLMNIIRNGS 510
Query: 434 MS-VSQILDDESYRDVADGDITNEMKVAKEAAKEIFKNVALPGNKFIEERDLLEFMIPEE 493
++ + + L D S D I +E + AK AA++IF NVA PG+KFI D++ F+ +E
Sbjct: 511 LTTLDEQLQDPSLDDDKGNQIRSEFE-AKLAARKIFHNVAKPGSKFIYANDIMRFLPDDE 570
Query: 494 VNLVWPHFE-VDKTRKIDMKALTNWVVKVYQGRKTLAHALKDTKTAVKQLNNLITALIIV 553
FE +T +I +L NWVV ++ R+ LA L DTKTAV +L+ ++ ++ +
Sbjct: 571 ALKTLSLFEGASETNRISKSSLKNWVVNAFRERRALALTLNDTKTAVNRLHKMVNIVVGI 630
Query: 554 VTAVIWLLLMEIATTKVLVFLLTQLAVAAFMFGNTCKTAFEALIFVFVMHPFDVGDRCVV 613
+ VIWL+++ I +TK LV + +Q+ V AF+FGN CK FE++I++FV+HPFDVGDRC +
Sbjct: 631 IILVIWLIILGITSTKFLVVMSSQVVVVAFIFGNMCKIVFESIIYLFVIHPFDVGDRCEI 690
Query: 614 DGVQLLVEEMNILTTVFLKLNNEKVYYPNSVLATKPITNYYRSPDMGDTVEFSIGFETPV 673
DGVQ++VEEMNILTTVFL+ +N+KV YPNS+L TK I NYYRSPDMGD +EFSI TP
Sbjct: 691 DGVQMVVEEMNILTTVFLRFDNQKVVYPNSLLWTKSIGNYYRSPDMGDGIEFSIHITTPA 750
Query: 674 ERIGAMKEQIKRYLEENPQHWYPNHNVVVKEIENVNKIKIALYTNHTMNFQDWAEKNQRR 716
E+I +K++I Y+E HWYP +V K++E++N ++IA++ H MN QD EK RR
Sbjct: 751 EKIILIKQRITSYIEGKKDHWYPAPMIVFKDMESLNSVRIAVWPTHRMNHQDMGEKWARR 810
BLAST of Cucsa.299750 vs. TAIR10
Match:
AT2G17000.1 (AT2G17000.1 Mechanosensitive ion channel family protein)
HSP 1 Score: 455.3 bits (1170), Expect = 7.0e-128
Identity = 260/664 (39.16%), Postives = 402/664 (60.54%), Query Frame = 1
Query: 96 SRFGEQSCFIDSDMLEEENHVSLREQIGATSS-RSSNLNTPKAQPEGEDDGNMGSNA--K 155
SR +S ID EE+ + S IG + RS L E EDD + + +
Sbjct: 167 SRAKARSRLIDPPQEEEQQYSSW---IGTSDQLRSGLLGRHSDDIEEEDDSSAEEDVPVE 226
Query: 156 HKKVKVK--TVIKWIGVFCIISCLVASLTVNPLKNRFLWGLKVWKWCLLATVILCGLIFT 215
++K+K+ T+++W+ + ++ LV SL ++ +N LW L +WKW ++ V++CG + +
Sbjct: 227 YRKLKMDAITLLQWMSLIALVVALVLSLGLHTWRNATLWSLHLWKWEVVLLVLICGRLVS 286
Query: 216 RWVMNVVVFLIERNFLLKKKVLYFVHGLKKSVQVTLWLSLVLATWGSLFDRRNHMISSSR 275
+ ++VF IERNFLL+K+VLYFV+G+K +VQ LWL LVL W LFD++ + S
Sbjct: 287 GCGIRIIVFFIERNFLLRKRVLYFVYGVKTAVQNCLWLGLVLLAWHFLFDKKVEKETQS- 346
Query: 276 ITAKILDAVTWTLASFLIGAFLWLIKTLLLKILASKFHMNRFFDRIQESLFLHHVLQTLL 335
+L ++ L FL+ LWLIKTL++K+LAS FH++ +FDRIQE+LF H++++TL
Sbjct: 347 ---DVLLLMSKILVCFLLSTVLWLIKTLVVKVLASSFHVSTYFDRIQEALFHHYLIETLS 406
Query: 336 RPPLVE------------DE--------STAKFRCCRFCFESKK----------PDRKKV 395
PP++E DE + C F +K P K
Sbjct: 407 GPPMLELSRIEEEEDRTQDEIYKMQKGGADLSPELCSAAFPQEKSGSTMNMKFSPIIPKT 466
Query: 396 -----IDMGKIHELKREKVSAWTMKVLVDAVTSSEMSVSQILDDESYRDVADGDITNEM- 455
I M +H++ ++ VSAW MK L+ V + +S LD+++ ++ + + T ++
Sbjct: 467 GSDNGITMDDLHKMNQKNVSAWNMKRLMKIVRNVSLST---LDEQALQNTCEDESTRQIR 526
Query: 456 --KVAKEAAKEIFKNVALPGNKFIEERDLLEFMIPEEVNLVWPHFE-VDKTRKIDMKALT 515
K AK AA++IFKNVA PG K I DL+ F+ +E FE T+KI AL
Sbjct: 527 SEKEAKAAARKIFKNVAQPGTKHIYLEDLMRFLRVDEAMKTMCLFEGALVTKKITKSALK 586
Query: 516 NWVVKVYQGRKTLAHALKDTKTAVKQLNNLITALIIVVTAVIWLLLMEIATTKVLVFLLT 575
NW+V ++ R+ LA L DTKTAV +L+++I+ L +V VIWL+L+EIAT+K L+FL +
Sbjct: 587 NWLVNAFRERRALALTLNDTKTAVNKLHHMISFLTAIVIIVIWLILLEIATSKYLLFLTS 646
Query: 576 QLAVAAFMFGNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNE 635
Q+ + AFMFGN+ KT FE++IF+F++HP+DVGDR ++D V+++VEEMNILTTVFL+ +N
Sbjct: 647 QVVLLAFMFGNSLKTVFESIIFLFIIHPYDVGDRLLIDTVEMVVEEMNILTTVFLRADNL 706
Query: 636 KVYYPNSVLATKPITNYYRSPDMGDTVEFSIGFETPVERIGAMKEQIKRYLEENPQHWYP 695
K+ YPN +L K I NY RSPDMGD V + TP E+I A+K++I Y++ P++WYP
Sbjct: 707 KIVYPNILLWQKAIHNYNRSPDMGDEVTCCVHITTPPEKIAAIKQRISSYIDSKPEYWYP 766
Query: 696 NHNVVVKEIENVNKIKIALYTNHTMNFQDWAEKNQRRTKLVMELKRIFEELKINYNLLPQ 716
+V+VK++E++N ++IA++ H +N Q+ E+ RR L+ E+ +I EL I Y P
Sbjct: 767 KADVIVKDVEDLNIVRIAIWLCHKINHQNMGERFTRRALLIEEVIKILLELDIQYRFHPL 820
BLAST of Cucsa.299750 vs. NCBI nr
Match:
gi|778696724|ref|XP_004141433.2| (PREDICTED: mechanosensitive ion channel protein 10 [Cucumis sativus])
HSP 1 Score: 1440.6 bits (3728), Expect = 0.0e+00
Identity = 720/721 (99.86%), Postives = 721/721 (100.00%), Query Frame = 1
Query: 1 MDVSGNKGIRRGSSQKESGNGGEVVVQLSGVENECSVPKQNRADSQTKEPTGSSVGYDNA 60
MDVSGNKGIRRGSSQKESGNGGEVVVQLSGVENECSVPKQNRADSQTKEPTGSSVGYDNA
Sbjct: 1 MDVSGNKGIRRGSSQKESGNGGEVVVQLSGVENECSVPKQNRADSQTKEPTGSSVGYDNA 60
Query: 61 SPLAPTPNKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQSCFIDSDMLEEENHVSLRE 120
SPLAPTPNKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQSCFIDSDMLEEENHVSLRE
Sbjct: 61 SPLAPTPNKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQSCFIDSDMLEEENHVSLRE 120
Query: 121 QIGATSSRSSNLNTPKAQPEGEDDGNMGSNAKHKKVKVKTVIKWIGVFCIISCLVASLTV 180
QIGATSSRSSNLNTPKAQPEGEDDGNMGSNAKHKKVKVKTVIKWIGVFCIISCLVASLTV
Sbjct: 121 QIGATSSRSSNLNTPKAQPEGEDDGNMGSNAKHKKVKVKTVIKWIGVFCIISCLVASLTV 180
Query: 181 NPLKNRFLWGLKVWKWCLLATVILCGLIFTRWVMNVVVFLIERNFLLKKKVLYFVHGLKK 240
NPLKNRFLWGLKVWKWCLLATVILCGLIFTRWVMNVVVFLIERNFLLKKKVLYFVHGLKK
Sbjct: 181 NPLKNRFLWGLKVWKWCLLATVILCGLIFTRWVMNVVVFLIERNFLLKKKVLYFVHGLKK 240
Query: 241 SVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASFLIGAFLWLIKTLLL 300
SVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASFLIGAFLWLIKTLLL
Sbjct: 241 SVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASFLIGAFLWLIKTLLL 300
Query: 301 KILASKFHMNRFFDRIQESLFLHHVLQTLLRPPLVEDESTAKFRCCRFCFESKKPDRKKV 360
KILASKFHMNRFFDRIQESLFLHHVLQTLLRPPLVEDESTAKFRCCRFCFESKKPDRKKV
Sbjct: 301 KILASKFHMNRFFDRIQESLFLHHVLQTLLRPPLVEDESTAKFRCCRFCFESKKPDRKKV 360
Query: 361 IDMGKIHELKREKVSAWTMKVLVDAVTSSEMSVSQILDDESYRDVADGDITNEMKVAKEA 420
IDMGKIHELKREKVSAWTMKVLVDAVTSSEMSVSQILDDESYRDVADGDITNEMKVAKEA
Sbjct: 361 IDMGKIHELKREKVSAWTMKVLVDAVTSSEMSVSQILDDESYRDVADGDITNEMKVAKEA 420
Query: 421 AKEIFKNVALPGNKFIEERDLLEFMIPEEVNLVWPHFEVDKTRKIDMKALTNWVVKVYQG 480
AKEIFKNVALPGNKFIEERDLLEFMIPEEVNLVWPHFEVDKTRKIDMKALTNWVVKVYQG
Sbjct: 421 AKEIFKNVALPGNKFIEERDLLEFMIPEEVNLVWPHFEVDKTRKIDMKALTNWVVKVYQG 480
Query: 481 RKTLAHALKDTKTAVKQLNNLITALIIVVTAVIWLLLMEIATTKVLVFLLTQLAVAAFMF 540
RKTLAHALKDTKTAVKQLNNLITALIIVVTAVIWLLLMEIATTKVLVFLLTQLAVAAFMF
Sbjct: 481 RKTLAHALKDTKTAVKQLNNLITALIIVVTAVIWLLLMEIATTKVLVFLLTQLAVAAFMF 540
Query: 541 GNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEKVYYPNSVL 600
GNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEKVYYPNSVL
Sbjct: 541 GNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEKVYYPNSVL 600
Query: 601 ATKPITNYYRSPDMGDTVEFSIGFETPVERIGAMKEQIKRYLEENPQHWYPNHNVVVKEI 660
ATKPITNYYRSPDMGDTVEFSIGFETPVERIGAMKEQIKRYLEENPQHWYPNHNVVVKEI
Sbjct: 601 ATKPITNYYRSPDMGDTVEFSIGFETPVERIGAMKEQIKRYLEENPQHWYPNHNVVVKEI 660
Query: 661 ENVNKIKIALYTNHTMNFQDWAEKNQRRTKLVMELKRIFEELKINYNLLPQTVHLFPVEG 720
ENVNKIKIALYTNHTMNFQDWAEKN+RRTKLVMELKRIFEELKINYNLLPQTVHLFPVEG
Sbjct: 661 ENVNKIKIALYTNHTMNFQDWAEKNRRRTKLVMELKRIFEELKINYNLLPQTVHLFPVEG 720
Query: 721 H 722
H
Sbjct: 721 H 721
BLAST of Cucsa.299750 vs. NCBI nr
Match:
gi|659104013|ref|XP_008452779.1| (PREDICTED: mechanosensitive ion channel protein 10-like [Cucumis melo])
HSP 1 Score: 1372.1 bits (3550), Expect = 0.0e+00
Identity = 687/721 (95.28%), Postives = 697/721 (96.67%), Query Frame = 1
Query: 1 MDVSGNKGIRRGSSQKESGNGGEVVVQLSGVENECSVPKQNRADSQTKEPTGSSVGYDNA 60
MDV+GNK IRR SSQKES NGGEVVVQLSGVENE SVPKQNR DSQTKEPTGSSVGY NA
Sbjct: 1 MDVNGNKPIRRSSSQKESENGGEVVVQLSGVENEYSVPKQNRVDSQTKEPTGSSVGYGNA 60
Query: 61 SPLAPTPNKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQSCFIDSDMLEEENHVSLRE 120
S LAPT NKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQ +IDSDM EEENHVSLRE
Sbjct: 61 SQLAPTANKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQPGYIDSDMFEEENHVSLRE 120
Query: 121 QIGATSSRSSNLNTPKAQPEGEDDGNMGSNAKHKKVKVKTVIKWIGVFCIISCLVASLTV 180
QIGATSSRSSNLNTPKAQPEGED NMG N KHKK KVKTV KWIGVFCIISCLVASLTV
Sbjct: 121 QIGATSSRSSNLNTPKAQPEGEDGRNMGLNEKHKKAKVKTVFKWIGVFCIISCLVASLTV 180
Query: 181 NPLKNRFLWGLKVWKWCLLATVILCGLIFTRWVMNVVVFLIERNFLLKKKVLYFVHGLKK 240
PLKNRFLWGLKVWKWCLLATVI CGLIFTRW+MNVVVFLIERNFLLKKKVLYFVHGLKK
Sbjct: 181 KPLKNRFLWGLKVWKWCLLATVIFCGLIFTRWIMNVVVFLIERNFLLKKKVLYFVHGLKK 240
Query: 241 SVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASFLIGAFLWLIKTLLL 300
SVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLAS LIGAFLWLIKTLLL
Sbjct: 241 SVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASLLIGAFLWLIKTLLL 300
Query: 301 KILASKFHMNRFFDRIQESLFLHHVLQTLLRPPLVEDESTAKFRCCRFCFESKKPDRKKV 360
KILASKFHMNRFFDRIQESLFLHHVLQTLLRPP VEDESTAKFRCCRFCFESK+ DRKKV
Sbjct: 301 KILASKFHMNRFFDRIQESLFLHHVLQTLLRPPPVEDESTAKFRCCRFCFESKESDRKKV 360
Query: 361 IDMGKIHELKREKVSAWTMKVLVDAVTSSEMSVSQILDDESYRDVADGDITNEMKVAKEA 420
IDMGKIH+LKREKVSAWTMKVLVDAVTSSEMS+SQILDDESYRDVADGDITNEM VAKEA
Sbjct: 361 IDMGKIHQLKREKVSAWTMKVLVDAVTSSEMSISQILDDESYRDVADGDITNEMNVAKEA 420
Query: 421 AKEIFKNVALPGNKFIEERDLLEFMIPEEVNLVWPHFEVDKTRKIDMKALTNWVVKVYQG 480
AKEIFKNVALPGNKFIEERDLL FMIPEEVNLVWPHFEVDKTRKIDMKALTNWVVKVYQG
Sbjct: 421 AKEIFKNVALPGNKFIEERDLLAFMIPEEVNLVWPHFEVDKTRKIDMKALTNWVVKVYQG 480
Query: 481 RKTLAHALKDTKTAVKQLNNLITALIIVVTAVIWLLLMEIATTKVLVFLLTQLAVAAFMF 540
RKTLAHALKDTKTAVKQLNNLITALII+VTA+IWLLLMEIATTKVLVFLLTQLAVAAFMF
Sbjct: 481 RKTLAHALKDTKTAVKQLNNLITALIIIVTAIIWLLLMEIATTKVLVFLLTQLAVAAFMF 540
Query: 541 GNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEKVYYPNSVL 600
GNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEKVYYPNSVL
Sbjct: 541 GNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEKVYYPNSVL 600
Query: 601 ATKPITNYYRSPDMGDTVEFSIGFETPVERIGAMKEQIKRYLEENPQHWYPNHNVVVKEI 660
ATKPITNYYRSPDMGDTVEFSIGFETPVERIGAMKEQIKRYLEENPQHWYPNHNVVVKEI
Sbjct: 601 ATKPITNYYRSPDMGDTVEFSIGFETPVERIGAMKEQIKRYLEENPQHWYPNHNVVVKEI 660
Query: 661 ENVNKIKIALYTNHTMNFQDWAEKNQRRTKLVMELKRIFEELKINYNLLPQTVHLFPVEG 720
ENVNKIKIALYTNHTMNFQDWAEKN+RRT+LVMELKRIFEELKINYNLLPQTVHLFPVEG
Sbjct: 661 ENVNKIKIALYTNHTMNFQDWAEKNRRRTELVMELKRIFEELKINYNLLPQTVHLFPVEG 720
Query: 721 H 722
H
Sbjct: 721 H 721
BLAST of Cucsa.299750 vs. NCBI nr
Match:
gi|778696721|ref|XP_004141434.2| (PREDICTED: mechanosensitive ion channel protein 10-like [Cucumis sativus])
HSP 1 Score: 1118.6 bits (2892), Expect = 0.0e+00
Identity = 575/723 (79.53%), Postives = 627/723 (86.72%), Query Frame = 1
Query: 1 MDVSG-NKGIRRGSSQKESGNGGEVVVQLSGVENECSVPKQNRADSQTKEPTGSSVGYDN 60
+D+S +K + R S KES NGG+ VV+LSGVEN SV +QN+ +SQT E SS+ Y N
Sbjct: 4 VDISNPSKVVPRSSPPKESENGGQFVVELSGVENGYSVQEQNKVNSQTNELIDSSISYGN 63
Query: 61 ASPLAPTPNKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQSCFIDSDMLEEENHVSLR 120
S LA NKP I SNG L LRR+ LSK KSRFG Q + DS+M EEEN+ S R
Sbjct: 64 DSQLA---NKPQNISSSNGNLI----LRRAILSKAKSRFGVQPVYTDSNMFEEENYPSSR 123
Query: 121 EQIGATSSRSSNLNTPKAQPEGEDDGNMGSNAKHKKVKVKTVIKWIGVFCIISCLVASLT 180
EQIG TSSR+ NT KA PE +D+ KHKKVKVKTVIKWIGVFCIISCLVASLT
Sbjct: 124 EQIGETSSRNFTHNTQKATPEKKDE-------KHKKVKVKTVIKWIGVFCIISCLVASLT 183
Query: 181 VNPLKNRFLWGLKVWKWCLLATVILCGLIFTRWVMNVVVFLIERNFLLKKKVLYFVHGLK 240
VNPLKNRFLWGLKVWKWCLLATVILCGL+FTRWVMNVVVFLIERNFLLKKKVLYFVHGLK
Sbjct: 184 VNPLKNRFLWGLKVWKWCLLATVILCGLLFTRWVMNVVVFLIERNFLLKKKVLYFVHGLK 243
Query: 241 KSVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASFLIGAFLWLIKTLL 300
KSVQVTLWL+LVLATW SLFDRRNHM+S+S I AK+LD VTWTL S LIGAFLWLIKTLL
Sbjct: 244 KSVQVTLWLTLVLATWESLFDRRNHMVSNSGINAKVLDLVTWTLVSLLIGAFLWLIKTLL 303
Query: 301 LKILASKFHMNRFFDRIQESLFLHHVLQTLLRPPLVEDESTAKFRCCRFCFESKKPDRKK 360
LKI+ASKFHMNRFFDRIQES+F HH+LQTLL EDES A+FRCCRF FESKK D +K
Sbjct: 304 LKIVASKFHMNRFFDRIQESIFHHHILQTLLMARTQEDESFAEFRCCRFSFESKKSDCQK 363
Query: 361 VIDMGKIHELKREKVSAWTMKVLVDAVTSSEMSVSQILDDESYRDVADGDITNEMKVAKE 420
I++ KI +LKREKVSAW MK LVDAVTSSEMS+S+ LD ESYR+ DG+IT+EMKVAK+
Sbjct: 364 AINIEKILQLKREKVSAWKMKTLVDAVTSSEMSISKTLD-ESYRNADDGEITDEMKVAKQ 423
Query: 421 AAKEIFKNVALPGNKFIEERDLLEFMIPE-EVNLVWPHFEVDKTRKIDMKALTNWVVKVY 480
AK+IFKNVA PG KFIEE+DLL+FMI E EVNL+WPHFEVDKT+KIDMK LTNWVVKVY
Sbjct: 424 TAKKIFKNVA-PGKKFIEEKDLLKFMIDEAEVNLLWPHFEVDKTKKIDMKGLTNWVVKVY 483
Query: 481 QGRKTLAHALKDTKTAVKQLNNLITALIIVVTAVIWLLLMEIATTKVLVFLLTQLAVAAF 540
QGRKTLAHALKDTKTAVKQLNNL+ ALI+VVTAVIWLLLMEIATTKVLVFLLTQLAVAAF
Sbjct: 484 QGRKTLAHALKDTKTAVKQLNNLVAALIVVVTAVIWLLLMEIATTKVLVFLLTQLAVAAF 543
Query: 541 MFGNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEKVYYPNS 600
MFGNTCK FE LIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEKVYYPNS
Sbjct: 544 MFGNTCKNTFEGLIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEKVYYPNS 603
Query: 601 VLATKPITNYYRSPDMGDTVEFSIGFETPVERIGAMKEQIKRYLEENPQHWYPNHNVVVK 660
VLATKPITNYYRSPDMGDT+EFSI F TP+E+IG MKE+IKRYLE+NPQHWYPNH+VVV+
Sbjct: 604 VLATKPITNYYRSPDMGDTIEFSISFTTPLEKIGVMKEKIKRYLEDNPQHWYPNHSVVVQ 663
Query: 661 EIENVNKIKIALYTNHTMNFQDWAEKNQRRTKLVMELKRIFEELKINYNLLPQTVHLFPV 720
EIENVNKIKIALYTNHTMNFQDW EKNQRRT+LVMELKRIFEELKINYNLLPQTVHLFP+
Sbjct: 664 EIENVNKIKIALYTNHTMNFQDWTEKNQRRTELVMELKRIFEELKINYNLLPQTVHLFPI 710
Query: 721 EGH 722
EGH
Sbjct: 724 EGH 710
BLAST of Cucsa.299750 vs. NCBI nr
Match:
gi|778696727|ref|XP_004141432.2| (PREDICTED: mechanosensitive ion channel protein 10 [Cucumis sativus])
HSP 1 Score: 1036.2 bits (2678), Expect = 2.7e-299
Identity = 534/738 (72.36%), Postives = 613/738 (83.06%), Query Frame = 1
Query: 1 MDVSGNKG--IRRGSSQKESGNGGEVVVQLSGVE----------NECSVPKQNRADSQTK 60
MDV+GNK +RR SSQKE N G+VVV++S N SV KQ+R DSQ K
Sbjct: 1 MDVNGNKPKVVRRSSSQKEGENAGQVVVEISNSSVVFSKETRDGNNYSVLKQDRVDSQNK 60
Query: 61 EPTGSSVGYDNASPLAPTPNKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQSCFIDSD 120
E TGSS+ + S + PT N+P KIP SN TLTPRRSL+RS LS+PKSRFGEQS + DSD
Sbjct: 61 ESTGSSIDHGFDSHIPPTANEPLKIPSSNRTLTPRRSLKRSILSRPKSRFGEQSRYTDSD 120
Query: 121 MLEEENHVSLREQIGATSSRSSNLNTPKAQPEGEDDGNMGS----NAKHKKVKVKTVIKW 180
EE H SLREQ GATSSRSS+LNTPKAQPE ED+ ++ N KHKK KVKTVIKW
Sbjct: 121 DKFEEKHESLREQTGATSSRSSSLNTPKAQPEEEDEEDIVKTEQLNKKHKKWKVKTVIKW 180
Query: 181 IGVFCIISCLVASLTVNPLKNRFLWGLKVWKWCLLATVILCGLIFTRWVMNVVVFLIERN 240
I VFC+I CLVASLTVN LKN F GL++WKWCLLATVI CGLI T W MNVVV LIE N
Sbjct: 181 IVVFCLIGCLVASLTVNRLKNCFFLGLEIWKWCLLATVIFCGLILTHWAMNVVVSLIEGN 240
Query: 241 FLLKKKVLYFVHGLKKSVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLA 300
FLLKKKVLYFVHGLKKSVQVTLWL+ VL+TW LF++RNH SSR T KILDA+TWTL
Sbjct: 241 FLLKKKVLYFVHGLKKSVQVTLWLASVLSTWEPLFNQRNHR--SSRTTGKILDAITWTLV 300
Query: 301 SFLIGAFLWLIKTLLLKILASKFHMNRFFDRIQESLFLHHVLQTLLRPPLVED-ESTAKF 360
+ LIG+FLWL+KTLLLKILASKFH +RFFDRIQES+F HHVLQ LL PPL+++ ES AKF
Sbjct: 301 ALLIGSFLWLVKTLLLKILASKFHKDRFFDRIQESIFHHHVLQALLGPPLMQEVESAAKF 360
Query: 361 RCCRFCFESKKPDRKKVIDMGKIHELKREKVSAWTMKVLVDAVTSSEMSVSQILDDESYR 420
C F +E+KK D KK+ID GKIH L+REKVS+WTMKVLV+AVTSS MS+SQILD ESY
Sbjct: 361 SRCLFSWENKKSDLKKIIDTGKIHHLQREKVSSWTMKVLVEAVTSSAMSISQILD-ESYY 420
Query: 421 DVADGDITNEMKVAKEAAKEIFKNVALPGNKFIEERDLLEFMIPEEVNLVWPHFEVDKTR 480
+V DG+I +EM++A A +I +NVALPG KFI+E DLL+F++ EE++LV PHFEVD+T+
Sbjct: 421 NVDDGEIDHEMEIASVVASKILRNVALPGKKFIQEEDLLQFVVKEEIDLVLPHFEVDETK 480
Query: 481 KIDMKALTNWVVKVYQGRKTLAHALKDTKTAVKQLNNLITALIIVVTAVIWLLLMEIATT 540
+I KAL WVVKV+Q RKTLAHALKDTKTAVKQLNNL+TA++I+V AVIWLLLMEIAT+
Sbjct: 481 RIGKKALKKWVVKVFQERKTLAHALKDTKTAVKQLNNLVTAVVIIVMAVIWLLLMEIATS 540
Query: 541 KVLVFLLTQLAVAAFMFGNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTT 600
KVLVFLL+QLAVAAFMFGN CKT FEALIFVFVMHPFDVGDRCVVDGV LLVEEMNILTT
Sbjct: 541 KVLVFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVDGVPLLVEEMNILTT 600
Query: 601 VFLKLNNEKVYYPNSVLATKPITNYYRSPDMGDTVEFSIGFETPVERIGAMKEQIKRYLE 660
VFLKLNNEKVYYPNSVLATKPI+NYYRSPDM +T EFSI F TP+ERIGAMKE+IKRYLE
Sbjct: 601 VFLKLNNEKVYYPNSVLATKPISNYYRSPDMSETTEFSINFATPLERIGAMKEKIKRYLE 660
Query: 661 ENPQHWYPNHNVVVKEIENVNKIKIALYTNHTMNFQDWAEKNQRRTKLVMELKRIFEELK 720
+NPQHW P+H +VVKEIENVN+IKIALY+ HTM++QD+ EK +RR++LVMELKRIFEELK
Sbjct: 661 KNPQHWRPSHLMVVKEIENVNEIKIALYSTHTMSYQDYGEKMKRRSELVMELKRIFEELK 720
Query: 721 INYNLLPQTVHLFPVEGH 722
INY LLPQT+HLFPVE H
Sbjct: 721 INYTLLPQTIHLFPVESH 735
BLAST of Cucsa.299750 vs. NCBI nr
Match:
gi|659104008|ref|XP_008452776.1| (PREDICTED: mechanosensitive ion channel protein 10-like [Cucumis melo])
HSP 1 Score: 951.8 bits (2459), Expect = 6.8e-274
Identity = 495/633 (78.20%), Postives = 543/633 (85.78%), Query Frame = 1
Query: 6 NKGIRRGSSQKESGNGGEVVVQLSGVENECSVPKQNRADSQTKEPTGSSVGYDNASPLAP 65
+K + R S KES +GG+ VV+LS +EN CSVP+QN SQT E SS+ YDN S LA
Sbjct: 10 SKVVPRSSFPKESEDGGQFVVELSSIENGCSVPEQNLG-SQTNEFIDSSISYDNDSQLA- 69
Query: 66 TPNKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQSCFIDSDMLEEENHVSLREQIGAT 125
NKP IP SNG LT LRR+ LSK KSRFG Q + DS+M EEEN+ S REQIG T
Sbjct: 70 --NKPQNIPSSNGNLT----LRRAILSKSKSRFGVQPVYTDSNMCEEENYPSSREQIGET 129
Query: 126 SSRSSNLNTPKAQPEGEDDGNMGSNAKHKKVKVKTVIKWIGVFCIISCLVASLTVNPLKN 185
SSRS NT KA PE +D+ KHKKVKVKTVIKWIGVFCIISCLVASLTV+PLKN
Sbjct: 130 SSRSFTHNTQKATPERKDE-------KHKKVKVKTVIKWIGVFCIISCLVASLTVDPLKN 189
Query: 186 RFLWGLKVWKWCLLATVILCGLIFTRWVMNVVVFLIERNFLLKKKVLYFVHGLKKSVQVT 245
RFLWGLKVWKWCLLATVILCGL+FTRWVMNVVVFLIE+NFLLKKKVLYFVHGLKKSVQVT
Sbjct: 190 RFLWGLKVWKWCLLATVILCGLLFTRWVMNVVVFLIEKNFLLKKKVLYFVHGLKKSVQVT 249
Query: 246 LWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASFLIGAFLWLIKTLLLKILAS 305
LWL+LV ATW SLFDRRNH +S+SRIT+K+LD VTWTL S LIGAFLWLIKTLLLKILAS
Sbjct: 250 LWLTLVFATWESLFDRRNHTVSNSRITSKVLDFVTWTLVSLLIGAFLWLIKTLLLKILAS 309
Query: 306 KFHMNRFFDRIQESLFLHHVLQTLLRPPLVEDESTAKFRCCRFCFESKKPDRKKVIDMGK 365
KFHMNRFFDRIQESLF HH+LQ LL EDES A+FRCCRF ESKK D +KVID+ K
Sbjct: 310 KFHMNRFFDRIQESLFHHHILQMLLMARTQEDESYAEFRCCRFPSESKKSDCQKVIDIEK 369
Query: 366 IHELKREKVSAWTMKVLVDAVTSSEMSVSQILDDESYRDVADGDITNEMKVAKEAAKEIF 425
IH+LKREKVSAW MK LVDAVTSSEMS+S+ L DESYR+ ADG+IT+EMKVAK+AAK+IF
Sbjct: 370 IHQLKREKVSAWKMKTLVDAVTSSEMSISKAL-DESYRNAADGEITDEMKVAKQAAKKIF 429
Query: 426 KNVALPGNKFIEERDLLEFMIPE-EVNLVWPHFEVDKTRKIDMKALTNWVVKVYQGRKTL 485
KNVA PG KF+EE+DLL+FMI E EVNL+WPHFEVDKT+KIDMKALTNWVVKVYQGRKTL
Sbjct: 430 KNVA-PGKKFLEEKDLLKFMIDEAEVNLLWPHFEVDKTKKIDMKALTNWVVKVYQGRKTL 489
Query: 486 AHALKDTKTAVKQLNNLITALIIVVTAVIWLLLMEIATTKVLVFLLTQLAVAAFMFGNTC 545
AHALKDTKTAVKQL+NL+ ALI++VTAVIWLLLMEIATTKVLVFLLTQ AVAAFMFGNTC
Sbjct: 490 AHALKDTKTAVKQLDNLVAALIVIVTAVIWLLLMEIATTKVLVFLLTQFAVAAFMFGNTC 549
Query: 546 KTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEKVYYPNSVLATKP 605
K FE LIFVFVMHPFDVGD CVVDG+QLLVEEMNILTTVFLKLNNEKVYYPNSVLATKP
Sbjct: 550 KNTFEGLIFVFVMHPFDVGDLCVVDGIQLLVEEMNILTTVFLKLNNEKVYYPNSVLATKP 609
Query: 606 ITNYYRSPDMGDTVEFSIGFETPVERIGAMKEQ 638
ITNYYRSPDMGDT+EFSI F TP+E+IG MKE+
Sbjct: 610 ITNYYRSPDMGDTIEFSISFTTPLEKIGIMKEK 625
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| MSL10_ARATH | 3.1e-202 | 52.30 | Mechanosensitive ion channel protein 10 OS=Arabidopsis thaliana GN=MSL10 PE=1 SV... | [more] |
| MSL9_ARATH | 1.0e-184 | 47.59 | Mechanosensitive ion channel protein 9 OS=Arabidopsis thaliana GN=MSL9 PE=1 SV=1 | [more] |
| MSL5_ARATH | 5.8e-132 | 41.26 | Mechanosensitive ion channel protein 5 OS=Arabidopsis thaliana GN=MSL5 PE=2 SV=1 | [more] |
| MSL6_ARATH | 1.3e-131 | 40.67 | Mechanosensitive ion channel protein 6 OS=Arabidopsis thaliana GN=MSL6 PE=1 SV=1 | [more] |
| MSL8_ARATH | 2.1e-129 | 40.23 | Mechanosensitive ion channel protein 8 OS=Arabidopsis thaliana GN=MSL8 PE=2 SV=2 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A0A0L5E6_CUCSA | 0.0e+00 | 99.86 | Uncharacterized protein OS=Cucumis sativus GN=Csa_4G647440 PE=4 SV=1 | [more] |
| A0A067KQE3_JATCU | 5.9e-232 | 56.15 | Uncharacterized protein OS=Jatropha curcas GN=JCGZ_07615 PE=4 SV=1 | [more] |
| A0A059CMY1_EUCGR | 3.1e-225 | 55.77 | Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_C00709 PE=4 SV=1 | [more] |
| B9RQV1_RICCO | 9.1e-225 | 56.18 | Putative uncharacterized protein OS=Ricinus communis GN=RCOM_0706730 PE=4 SV=1 | [more] |
| W9R6V9_9ROSA | 2.5e-222 | 56.97 | Mechanosensitive ion channel protein 10 OS=Morus notabilis GN=L484_018576 PE=4 S... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| AT5G12080.1 | 1.8e-203 | 52.30 | mechanosensitive channel of small conductance-like 10 | [more] |
| AT5G19520.1 | 5.7e-186 | 47.59 | mechanosensitive channel of small conductance-like 9 | [more] |
| AT3G14810.1 | 3.3e-133 | 41.26 | mechanosensitive channel of small conductance-like 5 | [more] |
| AT1G78610.1 | 7.3e-133 | 40.67 | mechanosensitive channel of small conductance-like 6 | [more] |
| AT2G17000.1 | 7.0e-128 | 39.16 | Mechanosensitive ion channel family protein | [more] |
