Cucsa.292810 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.292810 vs. Swiss-Prot
Match: PP345_ARATH (Pentatricopeptide repeat-containing protein At4g32430, mitochondrial OS=Arabidopsis thaliana GN=PCMP-E40 PE=2 SV=1)

HSP 1 Score: 196.4 bits (498), Expect = 3.9e-49
Identity = 105/256 (41.02%), Postives = 160/256 (62.50%), Query Frame = 1

Query: 2   YCKSGLLERAFSVFENLHDPDIVSWNTILSGFEKSENALSFARRINLNGVEFDSVTYSTA 61
           Y K G+LE   SVF  + + ++VSW T++S     ++A+S    +  +GV  + VT+   
Sbjct: 321 YSKCGVLEAVKSVFHQMSERNVVSWTTMISS--NKDDAVSIFLNMRFDGVYPNEVTFVGL 380

Query: 62  FSFCFDGKNFLFSWLLHTLALKCGFKGDVFVGNALVTMYWRWEHLVDARKVFDDMPSRDR 121
            +     +       +H L +K GF  +  VGN+ +T+Y ++E L DA+K F+D+  R+ 
Sbjct: 381 INAVKCNEQIKEGLKIHGLCIKTGFVSEPSVGNSFITLYAKFEALEDAKKAFEDITFREI 440

Query: 122 VLWSAMITDYAQEVNNGL--QAIFVFVQNVMEYKPNEYTFGSVLNAISAGEEDIPLKHGQ 181
           + W+AMI+ +AQ   NG   +A+ +F+    E  PNEYTFGSVLNAI A  EDI +K GQ
Sbjct: 441 ISWNAMISGFAQ---NGFSHEALKMFLSAAAETMPNEYTFGSVLNAI-AFAEDISVKQGQ 500

Query: 182 RCHSHLIKVGLNFDPIISGALLDMYAKRGSIQESQRVFNKTSKQSQLAWTVLIFDYAQHG 241
           RCH+HL+K+GLN  P++S ALLDMYAKRG+I ES++VFN+ S+++Q  WT +I  Y+ HG
Sbjct: 501 RCHAHLLKLGLNSCPVVSSALLDMYAKRGNIDESEKVFNEMSQKNQFVWTSIISAYSSHG 560

Query: 242 GLYFYVSFIEKYVFQN 256
                ++   K + +N
Sbjct: 561 DFETVMNLFHKMIKEN 570

HSP 2 Score: 156.8 bits (395), Expect = 3.4e-37
Identity = 84/233 (36.05%), Postives = 136/233 (58.37%), Query Frame = 1

Query: 1   MYCKSGLLERAFSVFENLHDPDIVSWNTILSGFEKSENALSFARRINLNGVEFDSVTYST 60
           MY K+G  + A  +FENL DPD+VSWNTILSGF+ ++ AL+F  R+   GV FD+ TYST
Sbjct: 120 MYRKAGRFDNALCIFENLVDPDVVSWNTILSGFDDNQIALNFVVRMKSAGVVFDAFTYST 179

Query: 61  AFSFCFDGKNFLFSWLLHTLALKCGFKGDVFVGNALVTMYWRWEHLVDARKVFDDMPSRD 120
           A SFC   + FL    L +  +K G + D+ VGN+ +TMY R      AR+VFD+M  +D
Sbjct: 180 ALSFCVGSEGFLLGLQLQSTVVKTGLESDLVVGNSFITMYSRSGSFRGARRVFDEMSFKD 239

Query: 121 RVLWSAMITDYAQEVNNGLQAIFVFVQNVME-YKPNEYTFGSVLNAISAGEEDIPLKHGQ 180
            + W+++++  +QE   G +A+ +F   + E  + +  +F SV+        +  LK  +
Sbjct: 240 MISWNSLLSGLSQEGTFGFEAVVIFRDMMREGVELDHVSFTSVITTCC---HETDLKLAR 299

Query: 181 RCHSHLIKVGLNFDPIISGALLDMYAKRGSIQESQRVFNKTSKQSQLAWTVLI 233
           + H   IK G      +   L+  Y+K G ++  + VF++ S+++ ++WT +I
Sbjct: 300 QIHGLCIKRGYESLLEVGNILMSRYSKCGVLEAVKSVFHQMSERNVVSWTTMI 349

HSP 3 Score: 113.2 bits (282), Expect = 4.3e-24
Identity = 67/244 (27.46%), Postives = 126/244 (51.64%), Query Frame = 1

Query: 1   MYCKSGLLERAFSVFENLHDPDIVSWNTILSGFEKSEN----ALSFARRINLNGVEFDSV 60
           MY +SG    A  VF+ +   D++SWN++LSG  +       A+   R +   GVE D V
Sbjct: 218 MYSRSGSFRGARRVFDEMSFKDMISWNSLLSGLSQEGTFGFEAVVIFRDMMREGVELDHV 277

Query: 61  TYSTAFSFCFDGKNFLFSWLLHTLALKCGFKGDVFVGNALVTMYWRWEHLVDARKVFDDM 120
           ++++  + C    +   +  +H L +K G++  + VGN L++ Y +   L   + VF  M
Sbjct: 278 SFTSVITTCCHETDLKLARQIHGLCIKRGYESLLEVGNILMSRYSKCGVLEAVKSVFHQM 337

Query: 121 PSRDRVLWSAMITDYAQEVNNGLQAIFVFVQNVME-YKPNEYTFGSVLNAISAGEEDIPL 180
             R+ V W+ MI+      +N   A+ +F+    +   PNE TF  ++NA+   E+   +
Sbjct: 338 SERNVVSWTTMIS------SNKDDAVSIFLNMRFDGVYPNEVTFVGLINAVKCNEQ---I 397

Query: 181 KHGQRCHSHLIKVGLNFDPIISGALLDMYAKRGSIQESQRVFNKTSKQSQLAWTVLIFDY 240
           K G + H   IK G   +P +  + + +YAK  +++++++ F   + +  ++W  +I  +
Sbjct: 398 KEGLKIHGLCIKTGFVSEPSVGNSFITLYAKFEALEDAKKAFEDITFREIISWNAMISGF 452

HSP 4 Score: 63.9 bits (154), Expect = 3.0e-09
Identity = 61/230 (26.52%), Postives = 110/230 (47.83%), Query Frame = 1

Query: 1   MYCKSGLLERAFSVFENLHDPDIVSWNTILSGF-------EKSENALSFARRINLNGVEF 60
           +Y K   LE A   FE++   +I+SWN ++SGF       E  +  LS A     N   F
Sbjct: 416 LYAKFEALEDAKKAFEDITFREIISWNAMISGFAQNGFSHEALKMFLSAAAETMPNEYTF 475

Query: 61  DSVTYSTAFSFCFDGKNFLFSWLLHTLALKCGFKGDVFVGNALVTMYWRWEHLVDARKVF 120
            SV  + AF+     K        H   LK G      V +AL+ MY +  ++ ++ KVF
Sbjct: 476 GSVLNAIAFAEDISVKQ---GQRCHAHLLKLGLNSCPVVSSALLDMYAKRGNIDESEKVF 535

Query: 121 DDMPSRDRVLWSAMITDYAQEVNNGLQAIFVFVQNVME-YKPNEYTFGSVLNAIS-AGEE 180
           ++M  +++ +W+++I+ Y+    +    + +F + + E   P+  TF SVL A +  G  
Sbjct: 536 NEMSQKNQFVWTSIISAYSSH-GDFETVMNLFHKMIKENVAPDLVTFLSVLTACNRKGMV 595

Query: 181 DIPLKHGQRCHSHLIKVGLNFDPIIS--GALLDMYAKRGSIQESQRVFNK 220
           D     G    + +I+V  N +P       ++DM  + G ++E++ + ++
Sbjct: 596 D----KGYEIFNMMIEV-YNLEPSHEHYSCMVDMLGRAGRLKEAEELMSE 636

HSP 5 Score: 63.5 bits (153), Expect = 3.9e-09
Identity = 47/194 (24.23%), Postives = 89/194 (45.88%), Query Frame = 1

Query: 50  GVEFDSVTYSTAFSFCFDGKNFLFSWLLHTLALKCGFKGDVFVGNALVTMYWRWEHLVDA 109
           G   D VT   A   C    +      +H  +   GF   V V NA++ MY +     +A
Sbjct: 73  GRHMDEVTLCLALKACRG--DLKRGCQIHGFSTTSGFTSFVCVSNAVMGMYRKAGRFDNA 132

Query: 110 RKVFDDMPSRDRVLWSAMITDYAQEVNNGLQAIFVFVQNVMEYKPNEYTFGSVLNAISAG 169
             +F+++   D V W+ +++ +    +N +   FV          + +T+ + L+    G
Sbjct: 133 LCIFENLVDPDVVSWNTILSGFD---DNQIALNFVVRMKSAGVVFDAFTYSTALS-FCVG 192

Query: 170 EEDIPLKHGQRCHSHLIKVGLNFDPIISGALLDMYAKRGSIQESQRVFNKTSKQSQLAWT 229
            E   L  G +  S ++K GL  D ++  + + MY++ GS + ++RVF++ S +  ++W 
Sbjct: 193 SEGFLL--GLQLQSTVVKTGLESDLVVGNSFITMYSRSGSFRGARRVFDEMSFKDMISWN 252

Query: 230 VLIFDYAQHGGLYF 244
            L+   +Q G   F
Sbjct: 253 SLLSGLSQEGTFGF 258

BLAST of Cucsa.292810 vs. Swiss-Prot
Match: PP277_ARATH (Pentatricopeptide repeat-containing protein At3g50420 OS=Arabidopsis thaliana GN=PCMP-E85 PE=2 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 2.5e-32
Identity = 81/260 (31.15%), Postives = 136/260 (52.31%), Query Frame = 1

Query: 1   MYCKSGLLERAFSVFENLHDPDIVSWNTILSGFEKS----ENALSFARRINLNGVEFDSV 60
           MYC  G +  AF VF  +H+P++VSWN+I+SG  ++    +  L + R + ++    D  
Sbjct: 344 MYCSCGDMREAFYVFGRIHNPNLVSWNSIISGCSENGFGEQAMLMYRRLLRMSTPRPDEY 403

Query: 61  TYSTAFSFCFDGKNFLFSWLLHTLALKCGFKGDVFVGNALVTMYWRWEHLVDARKVFDDM 120
           T+S A S   + + F+   LLH    K G++  VFVG  L++MY++      A+KVFD M
Sbjct: 404 TFSAAISATAEPERFVHGKLLHGQVTKLGYERSVFVGTTLLSMYFKNREAESAQKVFDVM 463

Query: 121 PSRDRVLWSAMITDYAQEVNNGLQAIFVFVQNVMEYKPNEYTFGSVLNAISAGEEDIPLK 180
             RD VLW+ MI  +++  N+ L   F       + + + ++  SV+ A S   +   L+
Sbjct: 464 KERDVVLWTEMIVGHSRLGNSELAVQFFIEMYREKNRSDGFSLSSVIGACS---DMAMLR 523

Query: 181 HGQRCHSHLIKVGLNFDPIISGALLDMYAKRGSIQESQRVFNKTSKQSQLAWTVLIFDYA 240
            G+  H   I+ G +    + GAL+DMY K G  + ++ +F+  S      W  ++  Y+
Sbjct: 524 QGEVFHCLAIRTGFDCVMSVCGALVDMYGKNGKYETAETIFSLASNPDLKCWNSMLGAYS 583

Query: 241 QHGGLYFYVSFIEKYVFQNF 257
           QHG +   +SF E+ +   F
Sbjct: 584 QHGMVEKALSFFEQILENGF 600

HSP 2 Score: 105.1 bits (261), Expect = 1.2e-21
Identity = 77/268 (28.73%), Postives = 132/268 (49.25%), Query Frame = 1

Query: 1   MYCKSGLLERAFSVFENLHDPDIVSWNTILSGF---EKSENALSFARRINLNGVEFDSVT 60
           MY   G LE A  +F+ +++ D V+WNT++ G    +K E+ L F R + ++GV+    T
Sbjct: 243 MYSSCGDLESARRIFDCVNNRDAVAWNTMIVGSLKNDKIEDGLMFFRNMLMSGVDPTQFT 302

Query: 61  YSTAFSFCFDGKNFLFSWLLHTLALKCGFKGDVFVGNALVTMYWRWEHLVDARKVFDDMP 120
           YS   + C    ++    L+H   +      D+ + NAL+ MY     + +A  VF  + 
Sbjct: 303 YSIVLNGCSKLGSYSLGKLIHARIIVSDSLADLPLDNALLDMYCSCGDMREAFYVFGRIH 362

Query: 121 SRDRVLWSAMITDYAQEVNNGLQAIFVF--VQNVMEYKPNEYTFGSVLNAISAGEEDIPL 180
           + + V W+++I+    E   G QA+ ++  +  +   +P+EYTF +   AISA  E    
Sbjct: 363 NPNLVSWNSIISG-CSENGFGEQAMLMYRRLLRMSTPRPDEYTFSA---AISATAEPERF 422

Query: 181 KHGQRCHSHLIKVGLNFDPIISGALLDMYAKRGSIQESQRVFNKTSKQSQLAWTVLIFDY 240
            HG+  H  + K+G      +   LL MY K    + +Q+VF+   ++  + WT +I  +
Sbjct: 423 VHGKLLHGQVTKLGYERSVFVGTTLLSMYFKNREAESAQKVFDVMKERDVVLWTEMIVGH 482

Query: 241 AQHGGLYFYVS-FIEKYVFQNFGFNFHL 263
           ++ G     V  FIE Y  +N    F L
Sbjct: 483 SRLGNSELAVQFFIEMYREKNRSDGFSL 506

HSP 3 Score: 91.7 bits (226), Expect = 1.3e-17
Identity = 64/246 (26.02%), Postives = 125/246 (50.81%), Query Frame = 1

Query: 1   MYCKSGLLERAFSVFENLHDPDIVSWNTILSGFEKSENALSFA----RRINLNGVEFDSV 60
           MY + G LE+A  VF+ +   ++VS+N + S + ++ +  S+A      +    V+ +S 
Sbjct: 141 MYVRCGSLEQARKVFDKMPHRNVVSYNALYSAYSRNPDFASYAFPLTTHMAFEYVKPNSS 200

Query: 61  TYSTAFSFCFDGKNFLFSWLLHTLALKCGFKGDVFVGNALVTMYWRWEHLVDARKVFDDM 120
           T+++    C   ++ L    L++  +K G+  +V V  +++ MY     L  AR++FD +
Sbjct: 201 TFTSLVQVCAVLEDVLMGSSLNSQIIKLGYSDNVVVQTSVLGMYSSCGDLESARRIFDCV 260

Query: 121 PSRDRVLWSAMITDYAQEVNNGLQAIFVFVQNVM--EYKPNEYTFGSVLNAIS-AGEEDI 180
            +RD V W+ MI    +  N+ ++   +F +N++     P ++T+  VLN  S  G   +
Sbjct: 261 NNRDAVAWNTMIVGSLK--NDKIEDGLMFFRNMLMSGVDPTQFTYSIVLNGCSKLGSYSL 320

Query: 181 PLKHGQRCHSHLIKVGLNFDPIISGALLDMYAKRGSIQESQRVFNKTSKQSQLAWTVLIF 240
               G+  H+ +I      D  +  ALLDMY   G ++E+  VF +    + ++W  +I 
Sbjct: 321 ----GKLIHARIIVSDSLADLPLDNALLDMYCSCGDMREAFYVFGRIHNPNLVSWNSIIS 380

HSP 4 Score: 77.0 bits (188), Expect = 3.4e-13
Identity = 48/160 (30.00%), Postives = 86/160 (53.75%), Query Frame = 1

Query: 77  LHTLALKCGFKGDV---FVGNALVTMYWRWEHLVDARKVFDDMPSRDRVLWSAMITDYAQ 136
           +H L L  G        +  N L++MY R   L  ARKVFD MP R+ V ++A+ + Y++
Sbjct: 116 IHALVLTAGAGAATESPYANNNLISMYVRCGSLEQARKVFDKMPHRNVVSYNALYSAYSR 175

Query: 137 EVNNGLQAIFVFVQNVMEY-KPNEYTFGSVLNAISAGEEDIPLKHGQRCHSHLIKVGLNF 196
             +    A  +      EY KPN  TF S++  + A  ED+ +  G   +S +IK+G + 
Sbjct: 176 NPDFASYAFPLTTHMAFEYVKPNSSTFTSLVQ-VCAVLEDVLM--GSSLNSQIIKLGYSD 235

Query: 197 DPIISGALLDMYAKRGSIQESQRVFNKTSKQSQLAWTVLI 233
           + ++  ++L MY+  G ++ ++R+F+  + +  +AW  +I
Sbjct: 236 NVVVQTSVLGMYSSCGDLESARRIFDCVNNRDAVAWNTMI 272

HSP 5 Score: 53.5 bits (127), Expect = 4.1e-06
Identity = 44/196 (22.45%), Postives = 88/196 (44.90%), Query Frame = 1

Query: 91  FVGNALVTMYWRWEHLVDARKVFDDMPSRDRVLWSAMITDYAQEVNNGLQAIFVFVQ--- 150
           +  N L++MY R   L  ARKVFD MP R+      ++T +      GL A+F +V    
Sbjct: 23  YANNNLISMYVRCSSLEQARKVFDKMPQRN------IVTLF------GLSAVFEYVSMGS 82

Query: 151 ----NVMEYKPNEYTFGSVLNAISAGEEDIP--------LKHGQRCHSHLIKVGLNF--- 210
                +++    +  F   LN I++   ++         LK  ++ H+ ++  G      
Sbjct: 83  SLHSQIIKLGSFQMIFFMPLNEIASSVVELTRKCVSITVLKRARQIHALVLTAGAGAATE 142

Query: 211 DPIISGALLDMYAKRGSIQESQRVFNKTSKQSQLAWTVLIFDYAQHGGLYFYVSFIEKYV 269
            P  +  L+ MY + GS++++++VF+K   ++ +++  L   Y+++     Y        
Sbjct: 143 SPYANNNLISMYVRCGSLEQARKVFDKMPHRNVVSYNALYSAYSRNPDFASYA------- 196

HSP 6 Score: 47.8 bits (112), Expect = 2.2e-04
Identity = 32/94 (34.04%), Postives = 48/94 (51.06%), Query Frame = 1

Query: 1   MYCKSGLLERAFSVFENLHDPDIVSWNTILSGFEKS---ENALSFARRINLNGVEFDSVT 60
           MY K+G  E A ++F    +PD+  WN++L  + +    E ALSF  +I  NG   D+VT
Sbjct: 547 MYGKNGKYETAETIFSLASNPDLKCWNSMLGAYSQHGMVEKALSFFEQILENGFMPDAVT 606

Query: 61  YSTAFSFCFDGKNFL---FSW-LLHTLALKCGFK 88
           Y +  + C    + L   F W  +    +K GFK
Sbjct: 607 YLSLLAACSHRGSTLQGKFLWNQMKEQGIKAGFK 640

BLAST of Cucsa.292810 vs. Swiss-Prot
Match: PP252_ARATH (Pentatricopeptide repeat-containing protein At3g24000, mitochondrial OS=Arabidopsis thaliana GN=PCMP-H87 PE=3 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 1.1e-30
Identity = 78/244 (31.97%), Postives = 134/244 (54.92%), Query Frame = 1

Query: 1   MYCKSGLLERAFSVFENLHDPDIVSWNTILSGFEKSE---NALSFARRINLNGVEFDSVT 60
           MY K G LE A  VFE +   D V+W T++SG+ + +   +AL F  ++   G   +  T
Sbjct: 104 MYAKCGSLEEARKVFEKMPQRDFVTWTTLISGYSQHDRPCDALLFFNQMLRFGYSPNEFT 163

Query: 61  YSTAFSFCFDGKNFLFSWLLHTLALKCGFKGDVFVGNALVTMYWRWEHLVDARKVFDDMP 120
            S+        +       LH   +KCGF  +V VG+AL+ +Y R+  + DA+ VFD + 
Sbjct: 164 LSSVIKAAAAERRGCCGHQLHGFCVKCGFDSNVHVGSALLDLYTRYGLMDDAQLVFDALE 223

Query: 121 SRDRVLWSAMITDYAQEVNNGLQAIFVFVQNVME--YKPNEYTFGSVLNAISAGEEDIPL 180
           SR+ V W+A+I  +A+   +G +      Q ++   ++P+ +++ S+  A S+      L
Sbjct: 224 SRNDVSWNALIAGHARR--SGTEKALELFQGMLRDGFRPSHFSYASLFGACSSTGF---L 283

Query: 181 KHGQRCHSHLIKVGLNFDPIISGALLDMYAKRGSIQESQRVFNKTSKQSQLAWTVLIFDY 240
           + G+  H+++IK G          LLDMYAK GSI +++++F++ +K+  ++W  L+  Y
Sbjct: 284 EQGKWVHAYMIKSGEKLVAFAGNTLLDMYAKSGSIHDARKIFDRLAKRDVVSWNSLLTAY 342

HSP 2 Score: 102.1 bits (253), Expect = 1.0e-20
Identity = 58/184 (31.52%), Postives = 97/184 (52.72%), Query Frame = 1

Query: 58  YSTAFSFCFDGKNFLFSWLLHTLALKCGFKGDVFVGNALVTMYWRWEHLVDARKVFDDMP 117
           Y+T    C   K  +   ++H   L+  F+ D+ +GN L+ MY +   L +ARKVF+ MP
Sbjct: 63  YNTLLKKCTVFKLLIQGRIVHAHILQSIFRHDIVMGNTLLNMYAKCGSLEEARKVFEKMP 122

Query: 118 SRDRVLWSAMITDYAQEVNNGLQAIFVFVQNV-MEYKPNEYTFGSVLNAISAGEEDIPLK 177
            RD V W+ +I+ Y+Q  +    A+  F Q +   Y PNE+T  SV+ A +A        
Sbjct: 123 QRDFVTWTTLISGYSQH-DRPCDALLFFNQMLRFGYSPNEFTLSSVIKAAAAERRGC--- 182

Query: 178 HGQRCHSHLIKVGLNFDPIISGALLDMYAKRGSIQESQRVFNKTSKQSQLAWTVLIFDYA 237
            G + H   +K G + +  +  ALLD+Y + G + ++Q VF+    ++ ++W  LI  +A
Sbjct: 183 CGHQLHGFCVKCGFDSNVHVGSALLDLYTRYGLMDDAQLVFDALESRNDVSWNALIAGHA 242

Query: 238 QHGG 241
           +  G
Sbjct: 243 RRSG 242

HSP 3 Score: 84.0 bits (206), Expect = 2.8e-15
Identity = 55/171 (32.16%), Postives = 90/171 (52.63%), Query Frame = 1

Query: 1   MYCKSGLLERAFSVFENLHDPDIVSWNTILSGFEK---SENALSFARRINLNGVEFDSVT 60
           +Y + GL++ A  VF+ L   + VSWN +++G  +   +E AL   + +  +G      +
Sbjct: 205 LYTRYGLMDDAQLVFDALESRNDVSWNALIAGHARRSGTEKALELFQGMLRDGFRPSHFS 264

Query: 61  YSTAFSFCFDGKNFLFSWLLHTLALKCGFKGDVFVGNALVTMYWRWEHLVDARKVFDDMP 120
           Y++ F  C           +H   +K G K   F GN L+ MY +   + DARK+FD + 
Sbjct: 265 YASLFGACSSTGFLEQGKWVHAYMIKSGEKLVAFAGNTLLDMYAKSGSIHDARKIFDRLA 324

Query: 121 SRDRVLWSAMITDYAQEVNNGLQAIFVFVQ-NVMEYKPNEYTFGSVLNAIS 168
            RD V W++++T YAQ    G +A++ F +   +  +PNE +F SVL A S
Sbjct: 325 KRDVVSWNSLLTAYAQH-GFGKEAVWWFEEMRRVGIRPNEISFLSVLTACS 374

BLAST of Cucsa.292810 vs. Swiss-Prot
Match: PP307_ARATH (Pentatricopeptide repeat-containing protein At4g13650 OS=Arabidopsis thaliana GN=PCMP-H42 PE=2 SV=2)

HSP 1 Score: 126.3 bits (316), Expect = 4.9e-28
Identity = 74/243 (30.45%), Postives = 131/243 (53.91%), Query Frame = 1

Query: 1   MYCKSGLLERAFSVFENLHDPDIVSWNTILSGFEK---SENALSFARRINLNGVEFDSVT 60
           MY K G L+ A+ +       D+VSW T+++G+ +    + AL+  R++   G+  D V 
Sbjct: 534 MYAKLGKLDTAWDILIRFAGKDVVSWTTMIAGYTQYNFDDKALTTFRQMLDRGIRSDEVG 593

Query: 61  YSTAFSFCFDGKNFLFSWLLHTLALKCGFKGDVFVGNALVTMYWRWEHLVDARKVFDDMP 120
            + A S C   +       +H  A   GF  D+   NALVT+Y R   + ++   F+   
Sbjct: 594 LTNAVSACAGLQALKEGQQIHAQACVSGFSSDLPFQNALVTLYSRCGKIEESYLAFEQTE 653

Query: 121 SRDRVLWSAMITDYAQEVNNGLQAIFVFVQNVME-YKPNEYTFGSVLNAISAGEEDIPLK 180
           + D + W+A+++ + Q  NN  +A+ VFV+   E    N +TFGS   A+ A  E   +K
Sbjct: 654 AGDNIAWNALVSGFQQSGNNE-EALRVFVRMNREGIDNNNFTFGS---AVKAASETANMK 713

Query: 181 HGQRCHSHLIKVGLNFDPIISGALLDMYAKRGSIQESQRVFNKTSKQSQLAWTVLIFDYA 240
            G++ H+ + K G + +  +  AL+ MYAK GSI ++++ F + S +++++W  +I  Y+
Sbjct: 714 QGKQVHAVITKTGYDSETEVCNALISMYAKCGSISDAEKQFLEVSTKNEVSWNAIINAYS 772

HSP 2 Score: 105.9 bits (263), Expect = 6.9e-22
Identity = 71/239 (29.71%), Postives = 125/239 (52.30%), Query Frame = 1

Query: 1   MYCKSGLLERAFSVFENLHDPDIVSWNTILSGFEKSE---NALSFARRINLNGVEFDSVT 60
           +Y ++G ++ A  VF+ L   D  SW  ++SG  K+E    A+     + + G+      
Sbjct: 231 LYSRNGFVDLARRVFDGLRLKDHSSWVAMISGLSKNECEAEAIRLFCDMYVLGIMPTPYA 290

Query: 61  YSTAFSFCFDGKNFLFSWLLHTLALKCGFKGDVFVGNALVTMYWRWEHLVDARKVFDDMP 120
           +S+  S C   ++      LH L LK GF  D +V NALV++Y+   +L+ A  +F +M 
Sbjct: 291 FSSVLSACKKIESLEIGEQLHGLVLKLGFSSDTYVCNALVSLYFHLGNLISAEHIFSNMS 350

Query: 121 SRDRVLWSAMITDYAQEVNNGLQAIFVFVQNVME-YKPNEYTFGSVLNAISAGEEDIPLK 180
            RD V ++ +I   +Q    G +A+ +F +  ++  +P+  T  S++ A SA   D  L 
Sbjct: 351 QRDAVTYNTLINGLSQ-CGYGEKAMELFKRMHLDGLEPDSNTLASLVVACSA---DGTLF 410

Query: 181 HGQRCHSHLIKVGLNFDPIISGALLDMYAKRGSIQESQRVFNKTSKQSQLAWTVLIFDY 236
            GQ+ H++  K+G   +  I GALL++YAK   I+ +   F +T  ++ + W V++  Y
Sbjct: 411 RGQQLHAYTTKLGFASNNKIEGALLNLYAKCADIETALDYFLETEVENVVLWNVMLVAY 465

HSP 3 Score: 88.6 bits (218), Expect = 1.1e-16
Identity = 63/238 (26.47%), Postives = 118/238 (49.58%), Query Frame = 1

Query: 1   MYCKSGLLERAFSVFENLHDPDIVSWNTILSGFEKS---ENALSFARRINLNGVEFDSVT 60
           +Y + G +E ++  FE     D ++WN ++SGF++S   E AL    R+N  G++ ++ T
Sbjct: 635 LYSRCGKIEESYLAFEQTEAGDNIAWNALVSGFQQSGNNEEALRVFVRMNREGIDNNNFT 694

Query: 61  YSTAFSFCFDGKNFLFSWLLHTLALKCGFKGDVFVGNALVTMYWRWEHLVDARKVFDDMP 120
           + +A     +  N      +H +  K G+  +  V NAL++MY +   + DA K F ++ 
Sbjct: 695 FGSAVKAASETANMKQGKQVHAVITKTGYDSETEVCNALISMYAKCGSISDAEKQFLEVS 754

Query: 121 SRDRVLWSAMITDYAQEVNNGLQAIFVFVQNV-MEYKPNEYTFGSVLNAIS-AGEEDIPL 180
           +++ V W+A+I  Y++    G +A+  F Q +    +PN  T   VL+A S  G  D  +
Sbjct: 755 TKNEVSWNAIINAYSKH-GFGSEALDSFDQMIHSNVRPNHVTLVGVLSACSHIGLVDKGI 814

Query: 181 KHGQRCHSHLIKVGLNFDPIISGALLDMYAKRGSIQESQRVFNKTS-KQSQLAWTVLI 233
            + +  +S   + GL+  P     ++DM  + G +  ++    +   K   L W  L+
Sbjct: 815 AYFESMNS---EYGLSPKPEHYVCVVDMLTRAGLLSRAKEFIQEMPIKPDALVWRTLL 868

HSP 4 Score: 39.3 bits (90), Expect = 7.9e-02
Identity = 17/62 (27.42%), Postives = 36/62 (58.06%), Query Frame = 1

Query: 175 LKHGQRCHSHLIKVGLNFDPIISGALLDMYAKRGSIQESQRVFNKTSKQSQLAWTVLIFD 234
           L  G++ HS ++K+GL+ +  +S  L D Y  +G +  + +VF++  +++   W  +I +
Sbjct: 101 LDEGRKLHSQILKLGLDSNGCLSEKLFDFYLFKGDLYGAFKVFDEMPERTIFTWNKMIKE 160

Query: 235 YA 237
            A
Sbjct: 161 LA 162

BLAST of Cucsa.292810 vs. Swiss-Prot
Match: PP398_ARATH (Pentatricopeptide repeat-containing protein At5g27110 OS=Arabidopsis thaliana GN=PCMP-E14 PE=2 SV=2)

HSP 1 Score: 124.8 bits (312), Expect = 1.4e-27
Identity = 82/261 (31.42%), Postives = 135/261 (51.72%), Query Frame = 1

Query: 1   MYCKSGLLERAFSVFENLHDPDIVSWNTILSGFEKS---ENALSFARRINLNGVEFDSVT 60
           MY K  L E +  VF+ + + D+ SWNT++S F +S   E AL    R+  +G E +SV+
Sbjct: 151 MYAKFNLFENSLQVFDEMPERDVASWNTVISCFYQSGEAEKALELFGRMESSGFEPNSVS 210

Query: 61  YSTAFSFCFDGKNFLFSWL-----LHTLALKCGFKGDVFVGNALVTMYWRWEHLVDARKV 120
            + A S C         WL     +H   +K GF+ D +V +ALV MY + + L  AR+V
Sbjct: 211 LTVAISACSR-----LLWLERGKEIHRKCVKKGFELDEYVNSALVDMYGKCDCLEVAREV 270

Query: 121 FDDMPSRDRVLWSAMITDYAQEVNNGLQAIFVFVQNVME-YKPNEYTFGSVLNAISAGEE 180
           F  MP +  V W++MI  Y  +  +    + +  + ++E  +P++ T  S+L A S    
Sbjct: 271 FQKMPRKSLVAWNSMIKGYVAK-GDSKSCVEILNRMIIEGTRPSQTTLTSILMACSRSRN 330

Query: 181 DIPLKHGQRCHSHLIKVGLNFDPIISGALLDMYAKRGSIQESQRVFNKTSKQSQLAWTVL 240
              L HG+  H ++I+  +N D  ++ +L+D+Y K G    ++ VF+KT K    +W V+
Sbjct: 331 ---LLHGKFIHGYVIRSVVNADIYVNCSLIDLYFKCGEANLAETVFSKTQKDVAESWNVM 390

Query: 241 IFDYAQHGGLYFYVSFIEKYV 253
           I  Y   G  +  V   ++ V
Sbjct: 391 ISSYISVGNWFKAVEVYDQMV 402

HSP 2 Score: 110.5 bits (275), Expect = 2.8e-23
Identity = 71/243 (29.22%), Postives = 121/243 (49.79%), Query Frame = 1

Query: 1   MYCKSGLLERAFSVFENLHDPDIVSWNTILSGFEK---SENALSFARRINLNGVEFDSVT 60
           MY K   LE A  VF+ +    +V+WN+++ G+     S++ +    R+ + G      T
Sbjct: 252 MYGKCDCLEVAREVFQKMPRKSLVAWNSMIKGYVAKGDSKSCVEILNRMIIEGTRPSQTT 311

Query: 61  YSTAFSFCFDGKNFLFSWLLHTLALKCGFKGDVFVGNALVTMYWRWEHLVDARKVFDDMP 120
            ++    C   +N L    +H   ++     D++V  +L+ +Y++      A  VF    
Sbjct: 312 LTSILMACSRSRNLLHGKFIHGYVIRSVVNADIYVNCSLIDLYFKCGEANLAETVFSKTQ 371

Query: 121 SRDRVLWSAMITDYAQEVNNGLQAIFVFVQNV-MEYKPNEYTFGSVLNAISAGEEDIPLK 180
                 W+ MI+ Y   V N  +A+ V+ Q V +  KP+  TF SVL A S   +   L+
Sbjct: 372 KDVAESWNVMISSYIS-VGNWFKAVEVYDQMVSVGVKPDVVTFTSVLPACS---QLAALE 431

Query: 181 HGQRCHSHLIKVGLNFDPIISGALLDMYAKRGSIQESQRVFNKTSKQSQLAWTVLIFDYA 240
            G++ H  + +  L  D ++  ALLDMY+K G+ +E+ R+FN   K+  ++WTV+I  Y 
Sbjct: 432 KGKQIHLSISESRLETDELLLSALLDMYSKCGNEKEAFRIFNSIPKKDVVSWTVMISAYG 490

HSP 3 Score: 104.8 bits (260), Expect = 1.5e-21
Identity = 73/247 (29.55%), Postives = 126/247 (51.01%), Query Frame = 1

Query: 14  VFENLH-DPDIVSWNTILSGFEKS----ENALSFARRINLNGVEFDSVTYSTAF-SFCFD 73
           VFEN     D+  WN+++SG+ K+    +    F R +N +    DS T+     ++   
Sbjct: 61  VFENFDIRSDVYIWNSLMSGYSKNSMFHDTLEVFKRLLNCSICVPDSFTFPNVIKAYGAL 120

Query: 74  GKNFLFSWLLHTLALKCGFKGDVFVGNALVTMYWRWEHLVDARKVFDDMPSRDRVLWSAM 133
           G+ FL   ++HTL +K G+  DV V ++LV MY ++    ++ +VFD+MP RD   W+ +
Sbjct: 121 GREFL-GRMIHTLVVKSGYVCDVVVASSLVGMYAKFNLFENSLQVFDEMPERDVASWNTV 180

Query: 134 ITDYAQEVNNGLQAIFVFVQNVMEYKPNEYTFGSVLNAISAGEEDIPLKHGQRCHSHLIK 193
           I+ + Q      +A+ +F +  ME    E    S+  AISA    + L+ G+  H   +K
Sbjct: 181 ISCFYQS-GEAEKALELFGR--MESSGFEPNSVSLTVAISACSRLLWLERGKEIHRKCVK 240

Query: 194 VGLNFDPIISGALLDMYAKRGSIQESQRVFNKTSKQSQLAWTVLIFDYAQHGGLYFYVSF 253
            G   D  ++ AL+DMY K   ++ ++ VF K  ++S +AW  +I  Y   G     V  
Sbjct: 241 KGFELDEYVNSALVDMYGKCDCLEVAREVFQKMPRKSLVAWNSMIKGYVAKGDSKSCVEI 300

Query: 254 IEKYVFQ 255
           + + + +
Sbjct: 301 LNRMIIE 303

HSP 4 Score: 66.2 bits (160), Expect = 6.1e-10
Identity = 43/167 (25.75%), Postives = 88/167 (52.69%), Query Frame = 1

Query: 76  LLHTLALKCGFKGDVFVGNALVTMYWRWEHLVDARKVFDDMPSRDRV-LWSAMITDYAQE 135
           L+H   L  G + DV +  +L+ +Y+  +    AR VF++   R  V +W+++++ Y++ 
Sbjct: 25  LVHQRILTLGLRRDVVLCKSLINVYFTCKDHCSARHVFENFDIRSDVYIWNSLMSGYSKN 84

Query: 136 -VNNGLQAIFVFVQNVMEYKPNEYTFGSVLNAISA-GEEDIPLKHGQRCHSHLIKVGLNF 195
            + +    +F  + N     P+ +TF +V+ A  A G E +    G+  H+ ++K G   
Sbjct: 85  SMFHDTLEVFKRLLNCSICVPDSFTFPNVIKAYGALGREFL----GRMIHTLVVKSGYVC 144

Query: 196 DPIISGALLDMYAKRGSIQESQRVFNKTSKQSQLAWTVLIFDYAQHG 240
           D +++ +L+ MYAK    + S +VF++  ++   +W  +I  + Q G
Sbjct: 145 DVVVASSLVGMYAKFNLFENSLQVFDEMPERDVASWNTVISCFYQSG 187

HSP 5 Score: 57.0 bits (136), Expect = 3.7e-07
Identity = 58/234 (24.79%), Postives = 105/234 (44.87%), Query Frame = 1

Query: 1   MYCKSGLLERAFSVFENLHDPDIVSWNTILSGFEKSEN---ALSFARRINLNGVEFDSVT 60
           +Y K G    A +VF         SWN ++S +    N   A+    ++   GV+ D VT
Sbjct: 353 LYFKCGEANLAETVFSKTQKDVAESWNVMISSYISVGNWFKAVEVYDQMVSVGVKPDVVT 412

Query: 61  YSTAFSFCFDGKNFLFSWLLHTLALKCGFKGDVFVGNALVTMYWRWEHLVDARKVFDDMP 120
           +++    C           +H    +   + D  + +AL+ MY +  +  +A ++F+ +P
Sbjct: 413 FTSVLPACSQLAALEKGKQIHLSISESRLETDELLLSALLDMYSKCGNEKEAFRIFNSIP 472

Query: 121 SRDRVLWSAMITDYAQEVNNGLQAIFVFVQ-NVMEYKPNEYTFGSVLNAIS-AGEEDIPL 180
            +D V W+ MI+ Y        +A++ F +      KP+  T  +VL+A   AG  D  L
Sbjct: 473 KKDVVSWTVMISAYGSH-GQPREALYQFDEMQKFGLKPDGVTLLAVLSACGHAGLIDEGL 532

Query: 181 KHGQRCHSHLIKVGLNFDPIIS--GALLDMYAKRGSIQESQRVFNKTSKQSQLA 228
           K   +  S   K G+  +PII     ++D+  + G + E+  +  +T + S  A
Sbjct: 533 KFFSQMRS---KYGI--EPIIEHYSCMIDILGRAGRLLEAYEIIQQTPETSDNA 580

BLAST of Cucsa.292810 vs. TrEMBL
Match: A0A0A0LWF3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G320370 PE=4 SV=1)

HSP 1 Score: 279.3 bits (713), Expect = 5.1e-72
Identity = 144/237 (60.76%), Postives = 168/237 (70.89%), Query Frame = 1

Query: 1   MYCKSGLLERAFSVFENLHDPDIVSWNTILSGFEKSENALSFARRINLNGVEFDSVTYST 60
           MYCKSG LERAFSVF+NLHDPDIVSWNTILSGFEKSENALSFA R+NLNGV+FDSVTY+T
Sbjct: 117 MYCKSGQLERAFSVFQNLHDPDIVSWNTILSGFEKSENALSFALRMNLNGVKFDSVTYTT 176

Query: 61  AFSFCFDGKNFLFSWLLHTLALKCGFKGDVFVGNALVTMYWRWEHLVDARKVFDDMPSRD 120
           A SFC DG+ FLF W LHTLALKCGFKGDVFVGNALVTMY RWEHLVDARKVFD+MPSRD
Sbjct: 177 ALSFCLDGEEFLFGWQLHTLALKCGFKGDVFVGNALVTMYSRWEHLVDARKVFDEMPSRD 236

Query: 121 RVLWSAMITDYAQEVNNGLQAIFVFVQNVMEYKPNEYTFGSVLNAISAGEEDIPLKHGQR 180
           RV WSAMIT YAQE +NGLQAIFVFVQN                    G   + +K G  
Sbjct: 237 RVSWSAMITGYAQEGDNGLQAIFVFVQN--------------------GIHCVAVKTGHE 296

Query: 181 CHSHLIKVGLNFDPIISGALLDMYAKRGSIQESQRVFNKTSKQSQLAWTVLIFDYAQ 238
            H+            +   L+  Y+K   I++++ VF   + ++ ++WT +I  Y +
Sbjct: 297 THTS-----------VGNVLISTYSKCEIIEDAKAVFELINDRNVISWTTMISLYEE 322

BLAST of Cucsa.292810 vs. TrEMBL
Match: A0A0A0LWF3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G320370 PE=4 SV=1)

HSP 1 Score: 233.0 bits (593), Expect = 4.2e-58
Identity = 131/254 (51.57%), Postives = 173/254 (68.11%), Query Frame = 1

Query: 2   YCKSGLLERAFSVFENLHDPDIVSWNTILSGFEKSENALSFARRINLNGVEFDSVTYSTA 61
           Y K  ++E A +VFE ++D +++SW T++S +E  E A+S   ++ L+GV  + VT    
Sbjct: 289 YSKCEIIEDAKAVFELINDRNVISWTTMISLYE--EGAVSLFNKMRLDGVYPNDVT---- 348

Query: 62  FSFCFDGKNFLFSWLLHTLALKCGFKGDVF---VGNALVTMYWRWEHLVDARKVFDDMPS 121
                      F  LLH + ++   +  +    VGN+L+TMY ++E + DA +VF ++P 
Sbjct: 349 -----------FIGLLHAITIRNMVEQGLMNLSVGNSLITMYAKFEFMQDASRVFIELPY 408

Query: 122 RDRVLWSAMITDYAQEVNNGL--QAIFVFVQNVMEYKPNEYTFGSVLNAISAGEEDIPLK 181
           R+ + W+A+I+ YAQ   N L  +A+  F+  +MEYKPNEYTFGSVLNAISAGE DI LK
Sbjct: 409 REIISWNALISGYAQ---NALCQEALEAFLYAIMEYKPNEYTFGSVLNAISAGE-DISLK 468

Query: 182 HGQRCHSHLIKVGLNFDPIISGALLDMYAKRGSIQESQRVFNKTSKQSQLAWTVLIFDYA 241
           HGQRCHSHLIKVGLN DPIISGALLDMYAKRGSIQESQRVFN+TSK+SQ AWT LI  YA
Sbjct: 469 HGQRCHSHLIKVGLNVDPIISGALLDMYAKRGSIQESQRVFNETSKESQFAWTALISGYA 521

Query: 242 QHGGLYFYVSFIEK 251
           QHG     +   E+
Sbjct: 529 QHGDYESVIKLFEE 521

HSP 2 Score: 57.8 bits (138), Expect = 2.4e-05
Identity = 52/227 (22.91%), Postives = 103/227 (45.37%), Query Frame = 1

Query: 1   MYCKSGLLERAFSVFENLHDPDIVSWNTILSGFEKSENALSFARRINLNGVEF--DSVTY 60
           MY K   ++ A  VF  L   +I+SWN ++SG+ ++              +E+  +  T+
Sbjct: 372 MYAKFEFMQDASRVFIELPYREIISWNALISGYAQNALCQEALEAFLYAIMEYKPNEYTF 431

Query: 61  STAFSFCFDGKNFLF--SWLLHTLALKCGFKGDVFVGNALVTMYWRWEHLVDARKVFDDM 120
            +  +    G++         H+  +K G   D  +  AL+ MY +   + ++++VF++ 
Sbjct: 432 GSVLNAISAGEDISLKHGQRCHSHLIKVGLNVDPIISGALLDMYAKRGSIQESQRVFNET 491

Query: 121 PSRDRVLWSAMITDYAQEVNNGLQAIFVFVQNVME-YKPNEYTFGSVLNAISAGEEDIPL 180
               +  W+A+I+ YAQ   +    I +F +   E  KP+   F SVL A S       +
Sbjct: 492 SKESQFAWTALISGYAQH-GDYESVIKLFEEMEKERIKPDAVIFLSVLTACSRNR---MV 551

Query: 181 KHGQRCHSHLIKVGLNFDPIIS------GALLDMYAKRGSIQESQRV 217
             G++  + +IK     D +I         ++DM  + G ++E++ +
Sbjct: 552 DMGRQFFNMMIK-----DHMIEPEGEHYSCMVDMLGRAGRLEEAEEI 589

HSP 3 Score: 207.6 bits (527), Expect = 1.9e-50
Identity = 104/232 (44.83%), Postives = 153/232 (65.95%), Query Frame = 1

Query: 1   MYCKSGLLERAFSVFENLHDPDIVSWNTILSGFEKSENALSFARRINLNGVEFDSVTYST 60
           MYCKSG  ++A  +F NL++PDIVSWNT+LSGFEKS++ALSFA R+NL GV FD+VTYST
Sbjct: 3   MYCKSGQFDKALCIFNNLNNPDIVSWNTVLSGFEKSDDALSFALRMNLIGVVFDAVTYST 62

Query: 61  AFSFCFDGKNFLFSWLLHTLALKCGFKGDVFVGNALVTMYWRWEHLVDARKVFDDMPSRD 120
           A SFC D + FLF   LH+L +K G   +V+VGNAL+TMY RW  LV+AR+VFD+MP++D
Sbjct: 63  ALSFCLDHEGFLFGLQLHSLIVKFGLDSEVYVGNALITMYSRWGRLVEARRVFDEMPNKD 122

Query: 121 RVLWSAMITDYAQEVNNGLQAIFVFVQNVMEYKPNEYTFGSVLNAISAGEEDIPLKHGQR 180
            V W+A+++ Y Q+ + G++AI   ++  M  K       S  +A SA   +  L+ G++
Sbjct: 123 SVSWNAILSGYTQDGDYGVEAILALIE--MMRKGLRLDHVSFTSAASACGHEKNLELGKQ 182

Query: 181 CHSHLIKVGLNFDPIISGALLDMYAKRGSIQESQRVFNKTSKQSQLAWTVLI 233
            H   IK+G      +   L+  Y+K     ++ +VF +   ++ ++WT +I
Sbjct: 183 IHGVSIKMGYGTHVSVGNVLMSTYSKCEVTGDANKVFRRMHDRNVISWTTMI 232

BLAST of Cucsa.292810 vs. TrEMBL
Match: A0A067GYB3_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g006364mg PE=4 SV=1)

HSP 1 Score: 201.8 bits (512), Expect = 1.0e-48
Identity = 111/240 (46.25%), Postives = 152/240 (63.33%), Query Frame = 1

Query: 2   YCKSGLLERAFSVFENLHDPDIVSWNTILSGFEKSENALSFARRINLNGVEFDSVTYSTA 61
           Y K  +   A  VF  +HD +++SW T++S     E+A+S  + + L+GV  + VT+   
Sbjct: 204 YSKCEVTGDANKVFRRMHDRNVISWTTMIS--MNREDAVSLFKEMRLDGVCPNDVTFIGL 263

Query: 62  FSFCFDGKNFLFSWLLHTLALKCGFKGDVFVGNALVTMYWRWEHLVDARKVFDDMPSRDR 121
                 G       ++H L +K  F  +  V N L+TMY R+E + D+ KVFD++  R+ 
Sbjct: 264 IHAISIGNLVKEGRMIHGLCIKTNFLSEPSVCNCLITMYARFESMQDSEKVFDELSCREI 323

Query: 122 VLWSAMITDYAQEVNNGLQ--AIFVFVQNVMEYKPNEYTFGSVLNAISAGEEDIPLKHGQ 181
           + W+A+I+ YAQ   NGL   A+  F   + E KPN YTFGSVLNA+ A E DI LKHGQ
Sbjct: 324 ISWNALISGYAQ---NGLSLAAVQAFFGVIKESKPNAYTFGSVLNAVGAAE-DISLKHGQ 383

Query: 182 RCHSHLIKVGLNFDPIISGALLDMYAKRGSIQESQRVFNKTSKQSQLAWTVLIFDYAQHG 240
           RCHSH+IKVGL+ DPI+  ALLDMY KRGSI ESQRVFN+T ++S+ AWT +I   A+HG
Sbjct: 384 RCHSHIIKVGLDSDPIVGSALLDMYGKRGSIFESQRVFNETQEKSEFAWTAIISALARHG 437

HSP 2 Score: 121.7 bits (304), Expect = 1.4e-24
Identity = 79/244 (32.38%), Postives = 130/244 (53.28%), Query Frame = 1

Query: 1   MYCKSGLLERAFSVFENLHDPDIVSWNTILSGFEKSEN----ALSFARRINLNGVEFDSV 60
           MY + G L  A  VF+ + + D VSWN ILSG+ +  +    A+     +   G+  D V
Sbjct: 101 MYSRWGRLVEARRVFDEMPNKDSVSWNAILSGYTQDGDYGVEAILALIEMMRKGLRLDHV 160

Query: 61  TYSTAFSFCFDGKNFLFSWLLHTLALKCGFKGDVFVGNALVTMYWRWEHLVDARKVFDDM 120
           ++++A S C   KN      +H +++K G+   V VGN L++ Y + E   DA KVF  M
Sbjct: 161 SFTSAASACGHEKNLELGKQIHGVSIKMGYGTHVSVGNVLMSTYSKCEVTGDANKVFRRM 220

Query: 121 PSRDRVLWSAMITDYAQEVNNGLQAIFVFVQNVME-YKPNEYTFGSVLNAISAGEEDIPL 180
             R+ + W+ MI+       N   A+ +F +  ++   PN+ TF  +++AIS G     +
Sbjct: 221 HDRNVISWTTMIS------MNREDAVSLFKEMRLDGVCPNDVTFIGLIHAISIGN---LV 280

Query: 181 KHGQRCHSHLIKVGLNFDPIISGALLDMYAKRGSIQESQRVFNKTSKQSQLAWTVLIFDY 240
           K G+  H   IK     +P +   L+ MYA+  S+Q+S++VF++ S +  ++W  LI  Y
Sbjct: 281 KEGRMIHGLCIKTNFLSEPSVCNCLITMYARFESMQDSEKVFDELSCREIISWNALISGY 335

HSP 3 Score: 59.7 bits (143), Expect = 6.3e-06
Identity = 52/223 (23.32%), Postives = 101/223 (45.29%), Query Frame = 1

Query: 1   MYCKSGLLERAFSVFENLHDPDIVSWNTILSGFEKSENALSFARRINLNGVEFDSVTYST 60
           MY +   ++ +  VF+ L   +I+SWN ++SG+  ++N LS A      GV  +S   + 
Sbjct: 299 MYARFESMQDSEKVFDELSCREIISWNALISGY--AQNGLSLAAVQAFFGVIKESKPNAY 358

Query: 61  AFSFCFDGK------NFLFSWLLHTLALKCGFKGDVFVGNALVTMYWRWEHLVDARKVFD 120
            F    +        +       H+  +K G   D  VG+AL+ MY +   + ++++VF+
Sbjct: 359 TFGSVLNAVGAAEDISLKHGQRCHSHIIKVGLDSDPIVGSALLDMYGKRGSIFESQRVFN 418

Query: 121 DMPSRDRVLWSAMITDYAQEVN-NGLQAIFVFVQNVMEYKPNEYTFGSVLNAISAGEEDI 180
           +   +    W+A+I+  A+  +   +   F  ++N    +P+  TF SVL     G   +
Sbjct: 419 ETQEKSEFAWTAIISALARHGDYESVMNQFKEMEN-KGVRPDSITFLSVLTV--CGRNGM 478

Query: 181 PLKHGQRCHSHLIKVGLNFDPIISGALLDMYAKRGSIQESQRV 217
             K      S L    +   P     ++DM  + G ++E++ +
Sbjct: 479 IHKGRHLFDSMLKDYHIEPSPDHYSCMVDMLGRVGRLEEAEEL 516

HSP 4 Score: 206.8 bits (525), Expect = 3.2e-50
Identity = 102/232 (43.97%), Postives = 152/232 (65.52%), Query Frame = 1

Query: 1   MYCKSGLLERAFSVFENLHDPDIVSWNTILSGFEKSENALSFARRINLNGVEFDSVTYST 60
           MY KSG  ++A  VFE L+DPD+VSWNT+LSGF+KSE AL+F  R+NLNGV FD+VT +T
Sbjct: 110 MYSKSGQFDKALCVFEGLNDPDVVSWNTLLSGFQKSEQALNFVLRMNLNGVAFDAVTCTT 169

Query: 61  AFSFCFDGKNFLFSWLLHTLALKCGFKGDVFVGNALVTMYWRWEHLVDARKVFDDMPSRD 120
           A SFC D + FL    LH L +K G   +VFVGNAL+TMY RW+ L +AR++FD+MP++D
Sbjct: 170 ALSFCLDLEGFLLGLQLHALVMKSGLDNEVFVGNALITMYARWKRLEEARRIFDEMPNKD 229

Query: 121 RVLWSAMITDYAQEVNNGLQAIFVFVQNVMEYKPNEYTFGSVLNAISAGEEDIPLKHGQR 180
            V W+A+++ Y+Q+   GL+AI  F++ V +    +    S   A+SA      L  G++
Sbjct: 230 LVSWNAILSGYSQDDGYGLEAICTFIEMVKQRMKLDNV--SFTGAVSACSHQRNLDVGRQ 289

Query: 181 CHSHLIKVGLNFDPIISGALLDMYAKRGSIQESQRVFNKTSKQSQLAWTVLI 233
            H   IK G      +   L+ MYAK   +++++ VF + ++++ ++WT +I
Sbjct: 290 IHGLCIKRGYGTHVSVCNVLMSMYAKCEVVEDAKLVFRRMNERNVISWTTMI 339

BLAST of Cucsa.292810 vs. TrEMBL
Match: A0A0D2QTV3_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_001G216100 PE=4 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 1.3e-43
Identity = 106/241 (43.98%), Postives = 153/241 (63.49%), Query Frame = 1

Query: 1   MYCKSGLLERAFSVFENLHDPDIVSWNTILSGFEKSENALSFARRINLNGVEFDSVTYST 60
           MY K  ++E A  VF  +++ +++SW T++S  EK  +AL     +  + V  + VTY  
Sbjct: 310 MYAKCEVVEDAKLVFRRMNERNVISWTTMISVDEK--DALHHFNEMRSDSVYPNDVTYVG 369

Query: 61  AFSFCFDGKNFLFSWLLHTLALKCGFKGDVFVGNALVTMYWRWEHLVDARKVFDDMPSRD 120
                  GK      ++H   +K  F  +  V N+L+TMY ++E + D+ KVF+++ S++
Sbjct: 370 LIHAITTGKLVEEGRMVHACCVKGNFLLESNVCNSLITMYAKFELIQDSIKVFEELESKE 429

Query: 121 RVLWSAMITDYAQEVNNGL--QAIFVFVQNVMEYKPNEYTFGSVLNAISAGEEDIPLKHG 180
            V W+A+I+ Y Q   NG+  +A+  F+    E KPN+YTFGSVLNAI + E  + LKHG
Sbjct: 430 IVSWNALISGYVQ---NGMLTEALRTFLSAARECKPNQYTFGSVLNAIGSCEH-VSLKHG 489

Query: 181 QRCHSHLIKVGLNFDPIISGALLDMYAKRGSIQESQRVFNKTSKQSQLAWTVLIFDYAQH 240
           Q CH HLIKVGLN DPIIS  LLDMYAKRGSI E+Q+VF++  ++SQ AWT LI  +A+H
Sbjct: 490 QWCHLHLIKVGLNTDPIISNGLLDMYAKRGSISEAQKVFSEIPQKSQFAWTSLISAHARH 544

HSP 2 Score: 104.8 bits (260), Expect = 1.7e-19
Identity = 70/248 (28.23%), Postives = 129/248 (52.02%), Query Frame = 1

Query: 1   MYCKSGLLERAFSVFENLHDPDIVSWNTILSGFEKSEN----ALSFARRINLNGVEFDSV 60
           MY +   LE A  +F+ + + D+VSWN ILSG+ + +     A+     +    ++ D+V
Sbjct: 208 MYARWKRLEEARRIFDEMPNKDLVSWNAILSGYSQDDGYGLEAICTFIEMVKQRMKLDNV 267

Query: 61  TYSTAFSFCFDGKNFLFSWLLHTLALKCGFKGDVFVGNALVTMYWRWEHLVDARKVFDDM 120
           +++ A S C   +N      +H L +K G+   V V N L++MY + E + DA+ VF  M
Sbjct: 268 SFTGAVSACSHQRNLDVGRQIHGLCIKRGYGTHVSVCNVLMSMYAKCEVVEDAKLVFRRM 327

Query: 121 PSRDRVLWSAMITDYAQEV---NNGLQAIFVFVQNVMEYKPNEYTFGSVLNAISAGEEDI 180
             R+ + W+ MI+   ++     N +++  V+        PN+ T+  +++AI+ G+   
Sbjct: 328 NERNVISWTTMISVDEKDALHHFNEMRSDSVY--------PNDVTYVGLIHAITTGK--- 387

Query: 181 PLKHGQRCHSHLIKVGLNFDPIISGALLDMYAKRGSIQESQRVFNKTSKQSQLAWTVLIF 240
            ++ G+  H+  +K     +  +  +L+ MYAK   IQ+S +VF +   +  ++W  LI 
Sbjct: 388 LVEEGRMVHACCVKGNFLLESNVCNSLITMYAKFELIQDSIKVFEELESKEIVSWNALIS 444

Query: 241 DYAQHGGL 242
            Y Q+G L
Sbjct: 448 GYVQNGML 444

HSP 3 Score: 80.1 bits (196), Expect = 4.5e-12
Identity = 61/227 (26.87%), Postives = 101/227 (44.49%), Query Frame = 1

Query: 1   MYCKSGLLERAFSVFENLHDPDIVSWNTILSGF-------EKSENALSFARRINLNGVEF 60
           MY K  L++ +  VFE L   +IVSWN ++SG+       E     LS AR    N   F
Sbjct: 406 MYAKFELIQDSIKVFEELESKEIVSWNALISGYVQNGMLTEALRTFLSAARECKPNQYTF 465

Query: 61  DSVTYSTAFSFCFDGKNFLFSWLLHTLALKCGFKGDVFVGNALVTMYWRWEHLVDARKVF 120
            SV    A   C +  +       H   +K G   D  + N L+ MY +   + +A+KVF
Sbjct: 466 GSVL--NAIGSC-EHVSLKHGQWCHLHLIKVGLNTDPIISNGLLDMYAKRGSISEAQKVF 525

Query: 121 DDMPSRDRVLWSAMITDYAQEVNNGLQAIFVFVQNVMEYKPNEYTFGSVLNAISAGEEDI 180
            ++P + +  W+++I+ +A+  N G              KP+  TF S+L A   G   +
Sbjct: 526 SEIPQKSQFAWTSLISAHARHGNYGSVMASFKEMKREGVKPDSITFLSILTA--CGRNGM 585

Query: 181 PLKHGQRCHSHLIKVGLNFDPIISGALLDMYAKRGSIQESQRVFNKT 221
               GQ   S L +  +         ++DM  + G ++E++++   T
Sbjct: 586 VDIGGQLFDSMLREYQIEPSSEHYSCMVDMLGRAGRLEEAEKLMGCT 627

HSP 4 Score: 203.8 bits (517), Expect = 2.7e-49
Identity = 104/232 (44.83%), Postives = 152/232 (65.52%), Query Frame = 1

Query: 1   MYCKSGLLERAFSVFENLHDPDIVSWNTILSGFEKSENALSFARRINLNGVEFDSVTYST 60
           MY KSG  ++A  +FE L+DPDIVSWNT+LSGF+KS+ AL+F  R+NL+GV FD+VT +T
Sbjct: 120 MYSKSGQFDKALCIFEGLNDPDIVSWNTLLSGFQKSDEALNFVLRMNLSGVVFDAVTCTT 179

Query: 61  AFSFCFDGKNFLFSWLLHTLALKCGFKGDVFVGNALVTMYWRWEHLVDARKVFDDMPSRD 120
           A SFCFD + FL    LHTL  K G   + FVGNAL+TMY RW+ L +AR+VFD+MP+RD
Sbjct: 180 ALSFCFDVEGFLLGLQLHTLVTKSGLDCEAFVGNALITMYSRWKRLEEARRVFDEMPNRD 239

Query: 121 RVLWSAMITDYAQEVNNGLQAIFVFVQNVMEYKPNEYTFGSVLNAISAGEEDIPLKHGQR 180
            V W++M++ Y+QE + GL+AI  F++ V +    +    S   A+SA      L+ G++
Sbjct: 240 LVSWNSMLSGYSQESSYGLEAISSFIEMVGQGMKLDNV--SFTGAVSACGHQRNLEVGRQ 299

Query: 181 CHSHLIKVGLNFDPIISGALLDMYAKRGSIQESQRVFNKTSKQSQLAWTVLI 233
            H   IK G      +   L+ MYAK    +++  VF + ++++ ++WT +I
Sbjct: 300 IHGLCIKRGYGTHVSVCNILMSMYAKCEVFEDATLVFERMNERNVISWTTMI 349

BLAST of Cucsa.292810 vs. TrEMBL
Match: A0A061GNQ5_THECC (Pentatricopeptide repeat (PPR) superfamily protein, putative OS=Theobroma cacao GN=TCM_038064 PE=4 SV=1)

HSP 1 Score: 191.4 bits (485), Expect = 1.4e-45
Identity = 107/241 (44.40%), Postives = 158/241 (65.56%), Query Frame = 1

Query: 1   MYCKSGLLERAFSVFENLHDPDIVSWNTILSGFEKSENALSFARRINLNGVEFDSVTYST 60
           MY K  + E A  VFE +++ +++SW T++S  E  E+A+     + L+GV  + VT+  
Sbjct: 320 MYAKCEVFEDATLVFERMNERNVISWTTMISIDE--EDAVRLFNEMRLDGVYPNDVTFVG 379

Query: 61  AFSFCFDGKNFLFSWLLHTLALKCGFKGDVFVGNALVTMYWRWEHLVDARKVFDDMPSRD 120
                   +      ++H   +K  F  +  V N+L+TMY ++  + D+ KVF+++  R+
Sbjct: 380 LIHAITTRELVEEGQMVHACCVKGNFLSESNVCNSLITMYAKFMLVQDSIKVFEELECRE 439

Query: 121 RVLWSAMITDYAQEVNNG--LQAIFVFVQNVMEYKPNEYTFGSVLNAISAGEEDIPLKHG 180
            V W+A+I+ YA+   NG  L+A+  F+  V E KPN+YTFGS LNAI +GE DI LKHG
Sbjct: 440 IVSWNALISCYAK---NGMCLEALRTFLSAVTECKPNQYTFGSALNAIGSGE-DISLKHG 499

Query: 181 QRCHSHLIKVGLNFDPIISGALLDMYAKRGSIQESQRVFNKTSKQSQLAWTVLIFDYAQH 240
           Q+CHSHLIKVG + DPI+S ALLDMYAKRGSI ES++VF++T ++SQ AWT +I  +A+H
Sbjct: 500 QQCHSHLIKVGFSTDPIVSNALLDMYAKRGSISESEKVFSETPQKSQFAWTSIISAHARH 554

HSP 2 Score: 105.9 bits (263), Expect = 7.7e-20
Identity = 72/244 (29.51%), Postives = 131/244 (53.69%), Query Frame = 1

Query: 1   MYCKSGLLERAFSVFENLHDPDIVSWNTILSGFEKSEN----ALSFARRINLNGVEFDSV 60
           MY +   LE A  VF+ + + D+VSWN++LSG+ +  +    A+S    +   G++ D+V
Sbjct: 218 MYSRWKRLEEARRVFDEMPNRDLVSWNSMLSGYSQESSYGLEAISSFIEMVGQGMKLDNV 277

Query: 61  TYSTAFSFCFDGKNFLFSWLLHTLALKCGFKGDVFVGNALVTMYWRWEHLVDARKVFDDM 120
           +++ A S C   +N      +H L +K G+   V V N L++MY + E   DA  VF+ M
Sbjct: 278 SFTGAVSACGHQRNLEVGRQIHGLCIKRGYGTHVSVCNILMSMYAKCEVFEDATLVFERM 337

Query: 121 PSRDRVLWSAMITDYAQEVNNGLQAIFVFVQNVME-YKPNEYTFGSVLNAISAGEEDIPL 180
             R+ + W+ MI+   ++      A+ +F +  ++   PN+ TF  +++AI+  E    +
Sbjct: 338 NERNVISWTTMISIDEED------AVRLFNEMRLDGVYPNDVTFVGLIHAITTREL---V 397

Query: 181 KHGQRCHSHLIKVGLNFDPIISGALLDMYAKRGSIQESQRVFNKTSKQSQLAWTVLIFDY 240
           + GQ  H+  +K     +  +  +L+ MYAK   +Q+S +VF +   +  ++W  LI  Y
Sbjct: 398 EEGQMVHACCVKGNFLSESNVCNSLITMYAKFMLVQDSIKVFEELECREIVSWNALISCY 452

HSP 3 Score: 71.2 bits (173), Expect = 2.1e-09
Identity = 55/225 (24.44%), Postives = 107/225 (47.56%), Query Frame = 1

Query: 1   MYCKSGLLERAFSVFENLHDPDIVSWNTILSGFEKSENALSFARRINLNGV---EFDSVT 60
           MY K  L++ +  VFE L   +IVSWN ++S + K+   L  A R  L+ V   + +  T
Sbjct: 416 MYAKFMLVQDSIKVFEELECREIVSWNALISCYAKNGMCLE-ALRTFLSAVTECKPNQYT 475

Query: 61  YSTAFSFCFDGKNFLFSW--LLHTLALKCGFKGDVFVGNALVTMYWRWEHLVDARKVFDD 120
           + +A +    G++         H+  +K GF  D  V NAL+ MY +   + ++ KVF +
Sbjct: 476 FGSALNAIGSGEDISLKHGQQCHSHLIKVGFSTDPIVSNALLDMYAKRGSISESEKVFSE 535

Query: 121 MPSRDRVLWSAMITDYAQEVNNGLQAIFVFVQNVMEYKPNEYTFGSVLNAISAGEEDIPL 180
            P + +  W+++I+ +A+  +              + +P+  TF SVL A   G   +  
Sbjct: 536 TPQKSQFAWTSIISAHARHGDYDSVMTSFKEMEREKVQPDSITFLSVLTA--CGRNGMVD 595

Query: 181 KHGQRCHSHLIKVGLNFDPIISGALLDMYAKRGSIQESQRVFNKT 221
              +   S L +  +    +    ++DM  + G ++E++++  ++
Sbjct: 596 MGRKLFDSMLAEYQIEPSSVHYSCMVDMLGRAGRLKEAEKLMGRS 637

HSP 4 Score: 201.1 bits (510), Expect = 1.8e-48
Identity = 108/256 (42.19%), Postives = 162/256 (63.28%), Query Frame = 1

Query: 2   YCKSGLLERAFSVFENLHDPDIVSWNTILSGFEKSENALSFARRINLNGVEFDSVTYSTA 61
           Y K G+LE   SVF  + + ++VSW T++S     ++A+S    + L+GV  + VT+   
Sbjct: 246 YSKCGVLEAVKSVFYQMSERNVVSWTTMISS--NRDDAVSIFLNMRLDGVYPNEVTFVGL 305

Query: 62  FSFCFDGKNFLFSWLLHTLALKCGFKGDVFVGNALVTMYWRWEHLVDARKVFDDMPSRDR 121
            +     +       +H L +K GF  +  VGN+ +TMY ++E L DA+K FDD+  R+ 
Sbjct: 306 LNAVKCNEQIKEGLKIHGLCIKTGFVSEPSVGNSFITMYAKFEALEDAKKAFDDITFREI 365

Query: 122 VLWSAMITDYAQEVNNGL--QAIFVFVQNVMEYKPNEYTFGSVLNAISAGEEDIPLKHGQ 181
           + W+AMI+ +AQ   NG   +A+ +F+    E  PNEYTFGSVLNAI A  EDI +KHGQ
Sbjct: 366 ISWNAMISGFAQ---NGFSHEALKMFLSATAETMPNEYTFGSVLNAI-AFAEDISVKHGQ 425

Query: 182 RCHSHLIKVGLNFDPIISGALLDMYAKRGSIQESQRVFNKTSKQSQLAWTVLIFDYAQHG 241
           RCH+HL+K+GLN  P++S ALLDMYAKRG+I ES++VFN+ S+++Q  WT +I  Y+ HG
Sbjct: 426 RCHAHLLKLGLNSCPVVSSALLDMYAKRGNINESEKVFNEMSQRNQFVWTSIISAYSSHG 485

Query: 242 GLYFYVSFIEKYVFQN 256
                ++   + + +N
Sbjct: 486 DFNSVMNLFHEMIKEN 495

BLAST of Cucsa.292810 vs. TAIR10
Match: AT4G32430.1 (AT4G32430.1 Pentatricopeptide repeat (PPR) superfamily protein)

HSP 1 Score: 196.4 bits (498), Expect = 2.2e-50
Identity = 105/256 (41.02%), Postives = 160/256 (62.50%), Query Frame = 1

Query: 2   YCKSGLLERAFSVFENLHDPDIVSWNTILSGFEKSENALSFARRINLNGVEFDSVTYSTA 61
           Y K G+LE   SVF  + + ++VSW T++S     ++A+S    +  +GV  + VT+   
Sbjct: 321 YSKCGVLEAVKSVFHQMSERNVVSWTTMISS--NKDDAVSIFLNMRFDGVYPNEVTFVGL 380

Query: 62  FSFCFDGKNFLFSWLLHTLALKCGFKGDVFVGNALVTMYWRWEHLVDARKVFDDMPSRDR 121
            +     +       +H L +K GF  +  VGN+ +T+Y ++E L DA+K F+D+  R+ 
Sbjct: 381 INAVKCNEQIKEGLKIHGLCIKTGFVSEPSVGNSFITLYAKFEALEDAKKAFEDITFREI 440

Query: 122 VLWSAMITDYAQEVNNGL--QAIFVFVQNVMEYKPNEYTFGSVLNAISAGEEDIPLKHGQ 181
           + W+AMI+ +AQ   NG   +A+ +F+    E  PNEYTFGSVLNAI A  EDI +K GQ
Sbjct: 441 ISWNAMISGFAQ---NGFSHEALKMFLSAAAETMPNEYTFGSVLNAI-AFAEDISVKQGQ 500

Query: 182 RCHSHLIKVGLNFDPIISGALLDMYAKRGSIQESQRVFNKTSKQSQLAWTVLIFDYAQHG 241
           RCH+HL+K+GLN  P++S ALLDMYAKRG+I ES++VFN+ S+++Q  WT +I  Y+ HG
Sbjct: 501 RCHAHLLKLGLNSCPVVSSALLDMYAKRGNIDESEKVFNEMSQKNQFVWTSIISAYSSHG 560

Query: 242 GLYFYVSFIEKYVFQN 256
                ++   K + +N
Sbjct: 561 DFETVMNLFHKMIKEN 570

HSP 2 Score: 156.8 bits (395), Expect = 1.9e-38
Identity = 84/233 (36.05%), Postives = 136/233 (58.37%), Query Frame = 1

Query: 1   MYCKSGLLERAFSVFENLHDPDIVSWNTILSGFEKSENALSFARRINLNGVEFDSVTYST 60
           MY K+G  + A  +FENL DPD+VSWNTILSGF+ ++ AL+F  R+   GV FD+ TYST
Sbjct: 120 MYRKAGRFDNALCIFENLVDPDVVSWNTILSGFDDNQIALNFVVRMKSAGVVFDAFTYST 179

Query: 61  AFSFCFDGKNFLFSWLLHTLALKCGFKGDVFVGNALVTMYWRWEHLVDARKVFDDMPSRD 120
           A SFC   + FL    L +  +K G + D+ VGN+ +TMY R      AR+VFD+M  +D
Sbjct: 180 ALSFCVGSEGFLLGLQLQSTVVKTGLESDLVVGNSFITMYSRSGSFRGARRVFDEMSFKD 239

Query: 121 RVLWSAMITDYAQEVNNGLQAIFVFVQNVME-YKPNEYTFGSVLNAISAGEEDIPLKHGQ 180
            + W+++++  +QE   G +A+ +F   + E  + +  +F SV+        +  LK  +
Sbjct: 240 MISWNSLLSGLSQEGTFGFEAVVIFRDMMREGVELDHVSFTSVITTCC---HETDLKLAR 299

Query: 181 RCHSHLIKVGLNFDPIISGALLDMYAKRGSIQESQRVFNKTSKQSQLAWTVLI 233
           + H   IK G      +   L+  Y+K G ++  + VF++ S+++ ++WT +I
Sbjct: 300 QIHGLCIKRGYESLLEVGNILMSRYSKCGVLEAVKSVFHQMSERNVVSWTTMI 349

HSP 3 Score: 113.2 bits (282), Expect = 2.4e-25
Identity = 67/244 (27.46%), Postives = 126/244 (51.64%), Query Frame = 1

Query: 1   MYCKSGLLERAFSVFENLHDPDIVSWNTILSGFEKSEN----ALSFARRINLNGVEFDSV 60
           MY +SG    A  VF+ +   D++SWN++LSG  +       A+   R +   GVE D V
Sbjct: 218 MYSRSGSFRGARRVFDEMSFKDMISWNSLLSGLSQEGTFGFEAVVIFRDMMREGVELDHV 277

Query: 61  TYSTAFSFCFDGKNFLFSWLLHTLALKCGFKGDVFVGNALVTMYWRWEHLVDARKVFDDM 120
           ++++  + C    +   +  +H L +K G++  + VGN L++ Y +   L   + VF  M
Sbjct: 278 SFTSVITTCCHETDLKLARQIHGLCIKRGYESLLEVGNILMSRYSKCGVLEAVKSVFHQM 337

Query: 121 PSRDRVLWSAMITDYAQEVNNGLQAIFVFVQNVME-YKPNEYTFGSVLNAISAGEEDIPL 180
             R+ V W+ MI+      +N   A+ +F+    +   PNE TF  ++NA+   E+   +
Sbjct: 338 SERNVVSWTTMIS------SNKDDAVSIFLNMRFDGVYPNEVTFVGLINAVKCNEQ---I 397

Query: 181 KHGQRCHSHLIKVGLNFDPIISGALLDMYAKRGSIQESQRVFNKTSKQSQLAWTVLIFDY 240
           K G + H   IK G   +P +  + + +YAK  +++++++ F   + +  ++W  +I  +
Sbjct: 398 KEGLKIHGLCIKTGFVSEPSVGNSFITLYAKFEALEDAKKAFEDITFREIISWNAMISGF 452

HSP 4 Score: 63.9 bits (154), Expect = 1.7e-10
Identity = 61/230 (26.52%), Postives = 110/230 (47.83%), Query Frame = 1

Query: 1   MYCKSGLLERAFSVFENLHDPDIVSWNTILSGF-------EKSENALSFARRINLNGVEF 60
           +Y K   LE A   FE++   +I+SWN ++SGF       E  +  LS A     N   F
Sbjct: 416 LYAKFEALEDAKKAFEDITFREIISWNAMISGFAQNGFSHEALKMFLSAAAETMPNEYTF 475

Query: 61  DSVTYSTAFSFCFDGKNFLFSWLLHTLALKCGFKGDVFVGNALVTMYWRWEHLVDARKVF 120
            SV  + AF+     K        H   LK G      V +AL+ MY +  ++ ++ KVF
Sbjct: 476 GSVLNAIAFAEDISVKQ---GQRCHAHLLKLGLNSCPVVSSALLDMYAKRGNIDESEKVF 535

Query: 121 DDMPSRDRVLWSAMITDYAQEVNNGLQAIFVFVQNVME-YKPNEYTFGSVLNAIS-AGEE 180
           ++M  +++ +W+++I+ Y+    +    + +F + + E   P+  TF SVL A +  G  
Sbjct: 536 NEMSQKNQFVWTSIISAYSSH-GDFETVMNLFHKMIKENVAPDLVTFLSVLTACNRKGMV 595

Query: 181 DIPLKHGQRCHSHLIKVGLNFDPIIS--GALLDMYAKRGSIQESQRVFNK 220
           D     G    + +I+V  N +P       ++DM  + G ++E++ + ++
Sbjct: 596 D----KGYEIFNMMIEV-YNLEPSHEHYSCMVDMLGRAGRLKEAEELMSE 636

HSP 5 Score: 63.5 bits (153), Expect = 2.2e-10
Identity = 47/194 (24.23%), Postives = 89/194 (45.88%), Query Frame = 1

Query: 50  GVEFDSVTYSTAFSFCFDGKNFLFSWLLHTLALKCGFKGDVFVGNALVTMYWRWEHLVDA 109
           G   D VT   A   C    +      +H  +   GF   V V NA++ MY +     +A
Sbjct: 73  GRHMDEVTLCLALKACRG--DLKRGCQIHGFSTTSGFTSFVCVSNAVMGMYRKAGRFDNA 132

Query: 110 RKVFDDMPSRDRVLWSAMITDYAQEVNNGLQAIFVFVQNVMEYKPNEYTFGSVLNAISAG 169
             +F+++   D V W+ +++ +    +N +   FV          + +T+ + L+    G
Sbjct: 133 LCIFENLVDPDVVSWNTILSGFD---DNQIALNFVVRMKSAGVVFDAFTYSTALS-FCVG 192

Query: 170 EEDIPLKHGQRCHSHLIKVGLNFDPIISGALLDMYAKRGSIQESQRVFNKTSKQSQLAWT 229
            E   L  G +  S ++K GL  D ++  + + MY++ GS + ++RVF++ S +  ++W 
Sbjct: 193 SEGFLL--GLQLQSTVVKTGLESDLVVGNSFITMYSRSGSFRGARRVFDEMSFKDMISWN 252

Query: 230 VLIFDYAQHGGLYF 244
            L+   +Q G   F
Sbjct: 253 SLLSGLSQEGTFGF 258

BLAST of Cucsa.292810 vs. TAIR10
Match: AT3G50420.1 (AT3G50420.1 Pentatricopeptide repeat (PPR) superfamily protein)

HSP 1 Score: 140.6 bits (353), Expect = 1.4e-33
Identity = 81/260 (31.15%), Postives = 136/260 (52.31%), Query Frame = 1

Query: 1   MYCKSGLLERAFSVFENLHDPDIVSWNTILSGFEKS----ENALSFARRINLNGVEFDSV 60
           MYC  G +  AF VF  +H+P++VSWN+I+SG  ++    +  L + R + ++    D  
Sbjct: 344 MYCSCGDMREAFYVFGRIHNPNLVSWNSIISGCSENGFGEQAMLMYRRLLRMSTPRPDEY 403

Query: 61  TYSTAFSFCFDGKNFLFSWLLHTLALKCGFKGDVFVGNALVTMYWRWEHLVDARKVFDDM 120
           T+S A S   + + F+   LLH    K G++  VFVG  L++MY++      A+KVFD M
Sbjct: 404 TFSAAISATAEPERFVHGKLLHGQVTKLGYERSVFVGTTLLSMYFKNREAESAQKVFDVM 463

Query: 121 PSRDRVLWSAMITDYAQEVNNGLQAIFVFVQNVMEYKPNEYTFGSVLNAISAGEEDIPLK 180
             RD VLW+ MI  +++  N+ L   F       + + + ++  SV+ A S   +   L+
Sbjct: 464 KERDVVLWTEMIVGHSRLGNSELAVQFFIEMYREKNRSDGFSLSSVIGACS---DMAMLR 523

Query: 181 HGQRCHSHLIKVGLNFDPIISGALLDMYAKRGSIQESQRVFNKTSKQSQLAWTVLIFDYA 240
            G+  H   I+ G +    + GAL+DMY K G  + ++ +F+  S      W  ++  Y+
Sbjct: 524 QGEVFHCLAIRTGFDCVMSVCGALVDMYGKNGKYETAETIFSLASNPDLKCWNSMLGAYS 583

Query: 241 QHGGLYFYVSFIEKYVFQNF 257
           QHG +   +SF E+ +   F
Sbjct: 584 QHGMVEKALSFFEQILENGF 600

HSP 2 Score: 105.1 bits (261), Expect = 6.6e-23
Identity = 77/268 (28.73%), Postives = 132/268 (49.25%), Query Frame = 1

Query: 1   MYCKSGLLERAFSVFENLHDPDIVSWNTILSGF---EKSENALSFARRINLNGVEFDSVT 60
           MY   G LE A  +F+ +++ D V+WNT++ G    +K E+ L F R + ++GV+    T
Sbjct: 243 MYSSCGDLESARRIFDCVNNRDAVAWNTMIVGSLKNDKIEDGLMFFRNMLMSGVDPTQFT 302

Query: 61  YSTAFSFCFDGKNFLFSWLLHTLALKCGFKGDVFVGNALVTMYWRWEHLVDARKVFDDMP 120
           YS   + C    ++    L+H   +      D+ + NAL+ MY     + +A  VF  + 
Sbjct: 303 YSIVLNGCSKLGSYSLGKLIHARIIVSDSLADLPLDNALLDMYCSCGDMREAFYVFGRIH 362

Query: 121 SRDRVLWSAMITDYAQEVNNGLQAIFVF--VQNVMEYKPNEYTFGSVLNAISAGEEDIPL 180
           + + V W+++I+    E   G QA+ ++  +  +   +P+EYTF +   AISA  E    
Sbjct: 363 NPNLVSWNSIISG-CSENGFGEQAMLMYRRLLRMSTPRPDEYTFSA---AISATAEPERF 422

Query: 181 KHGQRCHSHLIKVGLNFDPIISGALLDMYAKRGSIQESQRVFNKTSKQSQLAWTVLIFDY 240
            HG+  H  + K+G      +   LL MY K    + +Q+VF+   ++  + WT +I  +
Sbjct: 423 VHGKLLHGQVTKLGYERSVFVGTTLLSMYFKNREAESAQKVFDVMKERDVVLWTEMIVGH 482

Query: 241 AQHGGLYFYVS-FIEKYVFQNFGFNFHL 263
           ++ G     V  FIE Y  +N    F L
Sbjct: 483 SRLGNSELAVQFFIEMYREKNRSDGFSL 506

HSP 3 Score: 91.7 bits (226), Expect = 7.6e-19
Identity = 64/246 (26.02%), Postives = 125/246 (50.81%), Query Frame = 1

Query: 1   MYCKSGLLERAFSVFENLHDPDIVSWNTILSGFEKSENALSFA----RRINLNGVEFDSV 60
           MY + G LE+A  VF+ +   ++VS+N + S + ++ +  S+A      +    V+ +S 
Sbjct: 141 MYVRCGSLEQARKVFDKMPHRNVVSYNALYSAYSRNPDFASYAFPLTTHMAFEYVKPNSS 200

Query: 61  TYSTAFSFCFDGKNFLFSWLLHTLALKCGFKGDVFVGNALVTMYWRWEHLVDARKVFDDM 120
           T+++    C   ++ L    L++  +K G+  +V V  +++ MY     L  AR++FD +
Sbjct: 201 TFTSLVQVCAVLEDVLMGSSLNSQIIKLGYSDNVVVQTSVLGMYSSCGDLESARRIFDCV 260

Query: 121 PSRDRVLWSAMITDYAQEVNNGLQAIFVFVQNVM--EYKPNEYTFGSVLNAIS-AGEEDI 180
            +RD V W+ MI    +  N+ ++   +F +N++     P ++T+  VLN  S  G   +
Sbjct: 261 NNRDAVAWNTMIVGSLK--NDKIEDGLMFFRNMLMSGVDPTQFTYSIVLNGCSKLGSYSL 320

Query: 181 PLKHGQRCHSHLIKVGLNFDPIISGALLDMYAKRGSIQESQRVFNKTSKQSQLAWTVLIF 240
               G+  H+ +I      D  +  ALLDMY   G ++E+  VF +    + ++W  +I 
Sbjct: 321 ----GKLIHARIIVSDSLADLPLDNALLDMYCSCGDMREAFYVFGRIHNPNLVSWNSIIS 380

HSP 4 Score: 77.0 bits (188), Expect = 1.9e-14
Identity = 48/160 (30.00%), Postives = 86/160 (53.75%), Query Frame = 1

Query: 77  LHTLALKCGFKGDV---FVGNALVTMYWRWEHLVDARKVFDDMPSRDRVLWSAMITDYAQ 136
           +H L L  G        +  N L++MY R   L  ARKVFD MP R+ V ++A+ + Y++
Sbjct: 116 IHALVLTAGAGAATESPYANNNLISMYVRCGSLEQARKVFDKMPHRNVVSYNALYSAYSR 175

Query: 137 EVNNGLQAIFVFVQNVMEY-KPNEYTFGSVLNAISAGEEDIPLKHGQRCHSHLIKVGLNF 196
             +    A  +      EY KPN  TF S++  + A  ED+ +  G   +S +IK+G + 
Sbjct: 176 NPDFASYAFPLTTHMAFEYVKPNSSTFTSLVQ-VCAVLEDVLM--GSSLNSQIIKLGYSD 235

Query: 197 DPIISGALLDMYAKRGSIQESQRVFNKTSKQSQLAWTVLI 233
           + ++  ++L MY+  G ++ ++R+F+  + +  +AW  +I
Sbjct: 236 NVVVQTSVLGMYSSCGDLESARRIFDCVNNRDAVAWNTMI 272

HSP 5 Score: 53.5 bits (127), Expect = 2.3e-07
Identity = 44/196 (22.45%), Postives = 88/196 (44.90%), Query Frame = 1

Query: 91  FVGNALVTMYWRWEHLVDARKVFDDMPSRDRVLWSAMITDYAQEVNNGLQAIFVFVQ--- 150
           +  N L++MY R   L  ARKVFD MP R+      ++T +      GL A+F +V    
Sbjct: 23  YANNNLISMYVRCSSLEQARKVFDKMPQRN------IVTLF------GLSAVFEYVSMGS 82

Query: 151 ----NVMEYKPNEYTFGSVLNAISAGEEDIP--------LKHGQRCHSHLIKVGLNF--- 210
                +++    +  F   LN I++   ++         LK  ++ H+ ++  G      
Sbjct: 83  SLHSQIIKLGSFQMIFFMPLNEIASSVVELTRKCVSITVLKRARQIHALVLTAGAGAATE 142

Query: 211 DPIISGALLDMYAKRGSIQESQRVFNKTSKQSQLAWTVLIFDYAQHGGLYFYVSFIEKYV 269
            P  +  L+ MY + GS++++++VF+K   ++ +++  L   Y+++     Y        
Sbjct: 143 SPYANNNLISMYVRCGSLEQARKVFDKMPHRNVVSYNALYSAYSRNPDFASYA------- 196

HSP 6 Score: 47.8 bits (112), Expect = 1.3e-05
Identity = 32/94 (34.04%), Postives = 48/94 (51.06%), Query Frame = 1

Query: 1   MYCKSGLLERAFSVFENLHDPDIVSWNTILSGFEKS---ENALSFARRINLNGVEFDSVT 60
           MY K+G  E A ++F    +PD+  WN++L  + +    E ALSF  +I  NG   D+VT
Sbjct: 547 MYGKNGKYETAETIFSLASNPDLKCWNSMLGAYSQHGMVEKALSFFEQILENGFMPDAVT 606

Query: 61  YSTAFSFCFDGKNFL---FSW-LLHTLALKCGFK 88
           Y +  + C    + L   F W  +    +K GFK
Sbjct: 607 YLSLLAACSHRGSTLQGKFLWNQMKEQGIKAGFK 640

BLAST of Cucsa.292810 vs. TAIR10
Match: AT3G24000.1 (AT3G24000.1 Tetratricopeptide repeat (TPR)-like superfamily protein)

HSP 1 Score: 135.2 bits (339), Expect = 6.0e-32
Identity = 78/244 (31.97%), Postives = 134/244 (54.92%), Query Frame = 1

Query: 1   MYCKSGLLERAFSVFENLHDPDIVSWNTILSGFEKSE---NALSFARRINLNGVEFDSVT 60
           MY K G LE A  VFE +   D V+W T++SG+ + +   +AL F  ++   G   +  T
Sbjct: 104 MYAKCGSLEEARKVFEKMPQRDFVTWTTLISGYSQHDRPCDALLFFNQMLRFGYSPNEFT 163

Query: 61  YSTAFSFCFDGKNFLFSWLLHTLALKCGFKGDVFVGNALVTMYWRWEHLVDARKVFDDMP 120
            S+        +       LH   +KCGF  +V VG+AL+ +Y R+  + DA+ VFD + 
Sbjct: 164 LSSVIKAAAAERRGCCGHQLHGFCVKCGFDSNVHVGSALLDLYTRYGLMDDAQLVFDALE 223

Query: 121 SRDRVLWSAMITDYAQEVNNGLQAIFVFVQNVME--YKPNEYTFGSVLNAISAGEEDIPL 180
           SR+ V W+A+I  +A+   +G +      Q ++   ++P+ +++ S+  A S+      L
Sbjct: 224 SRNDVSWNALIAGHARR--SGTEKALELFQGMLRDGFRPSHFSYASLFGACSSTGF---L 283

Query: 181 KHGQRCHSHLIKVGLNFDPIISGALLDMYAKRGSIQESQRVFNKTSKQSQLAWTVLIFDY 240
           + G+  H+++IK G          LLDMYAK GSI +++++F++ +K+  ++W  L+  Y
Sbjct: 284 EQGKWVHAYMIKSGEKLVAFAGNTLLDMYAKSGSIHDARKIFDRLAKRDVVSWNSLLTAY 342

HSP 2 Score: 102.1 bits (253), Expect = 5.6e-22
Identity = 58/184 (31.52%), Postives = 97/184 (52.72%), Query Frame = 1

Query: 58  YSTAFSFCFDGKNFLFSWLLHTLALKCGFKGDVFVGNALVTMYWRWEHLVDARKVFDDMP 117
           Y+T    C   K  +   ++H   L+  F+ D+ +GN L+ MY +   L +ARKVF+ MP
Sbjct: 63  YNTLLKKCTVFKLLIQGRIVHAHILQSIFRHDIVMGNTLLNMYAKCGSLEEARKVFEKMP 122

Query: 118 SRDRVLWSAMITDYAQEVNNGLQAIFVFVQNV-MEYKPNEYTFGSVLNAISAGEEDIPLK 177
            RD V W+ +I+ Y+Q  +    A+  F Q +   Y PNE+T  SV+ A +A        
Sbjct: 123 QRDFVTWTTLISGYSQH-DRPCDALLFFNQMLRFGYSPNEFTLSSVIKAAAAERRGC--- 182

Query: 178 HGQRCHSHLIKVGLNFDPIISGALLDMYAKRGSIQESQRVFNKTSKQSQLAWTVLIFDYA 237
            G + H   +K G + +  +  ALLD+Y + G + ++Q VF+    ++ ++W  LI  +A
Sbjct: 183 CGHQLHGFCVKCGFDSNVHVGSALLDLYTRYGLMDDAQLVFDALESRNDVSWNALIAGHA 242

Query: 238 QHGG 241
           +  G
Sbjct: 243 RRSG 242

HSP 3 Score: 84.0 bits (206), Expect = 1.6e-16
Identity = 55/171 (32.16%), Postives = 90/171 (52.63%), Query Frame = 1

Query: 1   MYCKSGLLERAFSVFENLHDPDIVSWNTILSGFEK---SENALSFARRINLNGVEFDSVT 60
           +Y + GL++ A  VF+ L   + VSWN +++G  +   +E AL   + +  +G      +
Sbjct: 205 LYTRYGLMDDAQLVFDALESRNDVSWNALIAGHARRSGTEKALELFQGMLRDGFRPSHFS 264

Query: 61  YSTAFSFCFDGKNFLFSWLLHTLALKCGFKGDVFVGNALVTMYWRWEHLVDARKVFDDMP 120
           Y++ F  C           +H   +K G K   F GN L+ MY +   + DARK+FD + 
Sbjct: 265 YASLFGACSSTGFLEQGKWVHAYMIKSGEKLVAFAGNTLLDMYAKSGSIHDARKIFDRLA 324

Query: 121 SRDRVLWSAMITDYAQEVNNGLQAIFVFVQ-NVMEYKPNEYTFGSVLNAIS 168
            RD V W++++T YAQ    G +A++ F +   +  +PNE +F SVL A S
Sbjct: 325 KRDVVSWNSLLTAYAQH-GFGKEAVWWFEEMRRVGIRPNEISFLSVLTACS 374

BLAST of Cucsa.292810 vs. TAIR10
Match: AT4G13650.1 (AT4G13650.1 Pentatricopeptide repeat (PPR) superfamily protein)

HSP 1 Score: 126.3 bits (316), Expect = 2.8e-29
Identity = 74/243 (30.45%), Postives = 131/243 (53.91%), Query Frame = 1

Query: 1   MYCKSGLLERAFSVFENLHDPDIVSWNTILSGFEK---SENALSFARRINLNGVEFDSVT 60
           MY K G L+ A+ +       D+VSW T+++G+ +    + AL+  R++   G+  D V 
Sbjct: 534 MYAKLGKLDTAWDILIRFAGKDVVSWTTMIAGYTQYNFDDKALTTFRQMLDRGIRSDEVG 593

Query: 61  YSTAFSFCFDGKNFLFSWLLHTLALKCGFKGDVFVGNALVTMYWRWEHLVDARKVFDDMP 120
            + A S C   +       +H  A   GF  D+   NALVT+Y R   + ++   F+   
Sbjct: 594 LTNAVSACAGLQALKEGQQIHAQACVSGFSSDLPFQNALVTLYSRCGKIEESYLAFEQTE 653

Query: 121 SRDRVLWSAMITDYAQEVNNGLQAIFVFVQNVME-YKPNEYTFGSVLNAISAGEEDIPLK 180
           + D + W+A+++ + Q  NN  +A+ VFV+   E    N +TFGS   A+ A  E   +K
Sbjct: 654 AGDNIAWNALVSGFQQSGNNE-EALRVFVRMNREGIDNNNFTFGS---AVKAASETANMK 713

Query: 181 HGQRCHSHLIKVGLNFDPIISGALLDMYAKRGSIQESQRVFNKTSKQSQLAWTVLIFDYA 240
            G++ H+ + K G + +  +  AL+ MYAK GSI ++++ F + S +++++W  +I  Y+
Sbjct: 714 QGKQVHAVITKTGYDSETEVCNALISMYAKCGSISDAEKQFLEVSTKNEVSWNAIINAYS 772

HSP 2 Score: 105.9 bits (263), Expect = 3.9e-23
Identity = 71/239 (29.71%), Postives = 125/239 (52.30%), Query Frame = 1

Query: 1   MYCKSGLLERAFSVFENLHDPDIVSWNTILSGFEKSE---NALSFARRINLNGVEFDSVT 60
           +Y ++G ++ A  VF+ L   D  SW  ++SG  K+E    A+     + + G+      
Sbjct: 231 LYSRNGFVDLARRVFDGLRLKDHSSWVAMISGLSKNECEAEAIRLFCDMYVLGIMPTPYA 290

Query: 61  YSTAFSFCFDGKNFLFSWLLHTLALKCGFKGDVFVGNALVTMYWRWEHLVDARKVFDDMP 120
           +S+  S C   ++      LH L LK GF  D +V NALV++Y+   +L+ A  +F +M 
Sbjct: 291 FSSVLSACKKIESLEIGEQLHGLVLKLGFSSDTYVCNALVSLYFHLGNLISAEHIFSNMS 350

Query: 121 SRDRVLWSAMITDYAQEVNNGLQAIFVFVQNVME-YKPNEYTFGSVLNAISAGEEDIPLK 180
            RD V ++ +I   +Q    G +A+ +F +  ++  +P+  T  S++ A SA   D  L 
Sbjct: 351 QRDAVTYNTLINGLSQ-CGYGEKAMELFKRMHLDGLEPDSNTLASLVVACSA---DGTLF 410

Query: 181 HGQRCHSHLIKVGLNFDPIISGALLDMYAKRGSIQESQRVFNKTSKQSQLAWTVLIFDY 236
            GQ+ H++  K+G   +  I GALL++YAK   I+ +   F +T  ++ + W V++  Y
Sbjct: 411 RGQQLHAYTTKLGFASNNKIEGALLNLYAKCADIETALDYFLETEVENVVLWNVMLVAY 465

HSP 3 Score: 88.6 bits (218), Expect = 6.4e-18
Identity = 63/238 (26.47%), Postives = 118/238 (49.58%), Query Frame = 1

Query: 1   MYCKSGLLERAFSVFENLHDPDIVSWNTILSGFEKS---ENALSFARRINLNGVEFDSVT 60
           +Y + G +E ++  FE     D ++WN ++SGF++S   E AL    R+N  G++ ++ T
Sbjct: 635 LYSRCGKIEESYLAFEQTEAGDNIAWNALVSGFQQSGNNEEALRVFVRMNREGIDNNNFT 694

Query: 61  YSTAFSFCFDGKNFLFSWLLHTLALKCGFKGDVFVGNALVTMYWRWEHLVDARKVFDDMP 120
           + +A     +  N      +H +  K G+  +  V NAL++MY +   + DA K F ++ 
Sbjct: 695 FGSAVKAASETANMKQGKQVHAVITKTGYDSETEVCNALISMYAKCGSISDAEKQFLEVS 754

Query: 121 SRDRVLWSAMITDYAQEVNNGLQAIFVFVQNV-MEYKPNEYTFGSVLNAIS-AGEEDIPL 180
           +++ V W+A+I  Y++    G +A+  F Q +    +PN  T   VL+A S  G  D  +
Sbjct: 755 TKNEVSWNAIINAYSKH-GFGSEALDSFDQMIHSNVRPNHVTLVGVLSACSHIGLVDKGI 814

Query: 181 KHGQRCHSHLIKVGLNFDPIISGALLDMYAKRGSIQESQRVFNKTS-KQSQLAWTVLI 233
            + +  +S   + GL+  P     ++DM  + G +  ++    +   K   L W  L+
Sbjct: 815 AYFESMNS---EYGLSPKPEHYVCVVDMLTRAGLLSRAKEFIQEMPIKPDALVWRTLL 868

HSP 4 Score: 39.3 bits (90), Expect = 4.5e-03
Identity = 17/62 (27.42%), Postives = 36/62 (58.06%), Query Frame = 1

Query: 175 LKHGQRCHSHLIKVGLNFDPIISGALLDMYAKRGSIQESQRVFNKTSKQSQLAWTVLIFD 234
           L  G++ HS ++K+GL+ +  +S  L D Y  +G +  + +VF++  +++   W  +I +
Sbjct: 101 LDEGRKLHSQILKLGLDSNGCLSEKLFDFYLFKGDLYGAFKVFDEMPERTIFTWNKMIKE 160

Query: 235 YA 237
            A
Sbjct: 161 LA 162

BLAST of Cucsa.292810 vs. TAIR10
Match: AT5G27110.1 (AT5G27110.1 Tetratricopeptide repeat (TPR)-like superfamily protein)

HSP 1 Score: 124.8 bits (312), Expect = 8.1e-29
Identity = 82/261 (31.42%), Postives = 135/261 (51.72%), Query Frame = 1

Query: 1   MYCKSGLLERAFSVFENLHDPDIVSWNTILSGFEKS---ENALSFARRINLNGVEFDSVT 60
           MY K  L E +  VF+ + + D+ SWNT++S F +S   E AL    R+  +G E +SV+
Sbjct: 151 MYAKFNLFENSLQVFDEMPERDVASWNTVISCFYQSGEAEKALELFGRMESSGFEPNSVS 210

Query: 61  YSTAFSFCFDGKNFLFSWL-----LHTLALKCGFKGDVFVGNALVTMYWRWEHLVDARKV 120
            + A S C         WL     +H   +K GF+ D +V +ALV MY + + L  AR+V
Sbjct: 211 LTVAISACSR-----LLWLERGKEIHRKCVKKGFELDEYVNSALVDMYGKCDCLEVAREV 270

Query: 121 FDDMPSRDRVLWSAMITDYAQEVNNGLQAIFVFVQNVME-YKPNEYTFGSVLNAISAGEE 180
           F  MP +  V W++MI  Y  +  +    + +  + ++E  +P++ T  S+L A S    
Sbjct: 271 FQKMPRKSLVAWNSMIKGYVAK-GDSKSCVEILNRMIIEGTRPSQTTLTSILMACSRSRN 330

Query: 181 DIPLKHGQRCHSHLIKVGLNFDPIISGALLDMYAKRGSIQESQRVFNKTSKQSQLAWTVL 240
              L HG+  H ++I+  +N D  ++ +L+D+Y K G    ++ VF+KT K    +W V+
Sbjct: 331 ---LLHGKFIHGYVIRSVVNADIYVNCSLIDLYFKCGEANLAETVFSKTQKDVAESWNVM 390

Query: 241 IFDYAQHGGLYFYVSFIEKYV 253
           I  Y   G  +  V   ++ V
Sbjct: 391 ISSYISVGNWFKAVEVYDQMV 402

HSP 2 Score: 110.5 bits (275), Expect = 1.6e-24
Identity = 71/243 (29.22%), Postives = 121/243 (49.79%), Query Frame = 1

Query: 1   MYCKSGLLERAFSVFENLHDPDIVSWNTILSGFEK---SENALSFARRINLNGVEFDSVT 60
           MY K   LE A  VF+ +    +V+WN+++ G+     S++ +    R+ + G      T
Sbjct: 252 MYGKCDCLEVAREVFQKMPRKSLVAWNSMIKGYVAKGDSKSCVEILNRMIIEGTRPSQTT 311

Query: 61  YSTAFSFCFDGKNFLFSWLLHTLALKCGFKGDVFVGNALVTMYWRWEHLVDARKVFDDMP 120
            ++    C   +N L    +H   ++     D++V  +L+ +Y++      A  VF    
Sbjct: 312 LTSILMACSRSRNLLHGKFIHGYVIRSVVNADIYVNCSLIDLYFKCGEANLAETVFSKTQ 371

Query: 121 SRDRVLWSAMITDYAQEVNNGLQAIFVFVQNV-MEYKPNEYTFGSVLNAISAGEEDIPLK 180
                 W+ MI+ Y   V N  +A+ V+ Q V +  KP+  TF SVL A S   +   L+
Sbjct: 372 KDVAESWNVMISSYIS-VGNWFKAVEVYDQMVSVGVKPDVVTFTSVLPACS---QLAALE 431

Query: 181 HGQRCHSHLIKVGLNFDPIISGALLDMYAKRGSIQESQRVFNKTSKQSQLAWTVLIFDYA 240
            G++ H  + +  L  D ++  ALLDMY+K G+ +E+ R+FN   K+  ++WTV+I  Y 
Sbjct: 432 KGKQIHLSISESRLETDELLLSALLDMYSKCGNEKEAFRIFNSIPKKDVVSWTVMISAYG 490

HSP 3 Score: 104.8 bits (260), Expect = 8.7e-23
Identity = 73/247 (29.55%), Postives = 126/247 (51.01%), Query Frame = 1

Query: 14  VFENLH-DPDIVSWNTILSGFEKS----ENALSFARRINLNGVEFDSVTYSTAF-SFCFD 73
           VFEN     D+  WN+++SG+ K+    +    F R +N +    DS T+     ++   
Sbjct: 61  VFENFDIRSDVYIWNSLMSGYSKNSMFHDTLEVFKRLLNCSICVPDSFTFPNVIKAYGAL 120

Query: 74  GKNFLFSWLLHTLALKCGFKGDVFVGNALVTMYWRWEHLVDARKVFDDMPSRDRVLWSAM 133
           G+ FL   ++HTL +K G+  DV V ++LV MY ++    ++ +VFD+MP RD   W+ +
Sbjct: 121 GREFL-GRMIHTLVVKSGYVCDVVVASSLVGMYAKFNLFENSLQVFDEMPERDVASWNTV 180

Query: 134 ITDYAQEVNNGLQAIFVFVQNVMEYKPNEYTFGSVLNAISAGEEDIPLKHGQRCHSHLIK 193
           I+ + Q      +A+ +F +  ME    E    S+  AISA    + L+ G+  H   +K
Sbjct: 181 ISCFYQS-GEAEKALELFGR--MESSGFEPNSVSLTVAISACSRLLWLERGKEIHRKCVK 240

Query: 194 VGLNFDPIISGALLDMYAKRGSIQESQRVFNKTSKQSQLAWTVLIFDYAQHGGLYFYVSF 253
            G   D  ++ AL+DMY K   ++ ++ VF K  ++S +AW  +I  Y   G     V  
Sbjct: 241 KGFELDEYVNSALVDMYGKCDCLEVAREVFQKMPRKSLVAWNSMIKGYVAKGDSKSCVEI 300

Query: 254 IEKYVFQ 255
           + + + +
Sbjct: 301 LNRMIIE 303

HSP 4 Score: 66.2 bits (160), Expect = 3.4e-11
Identity = 43/167 (25.75%), Postives = 88/167 (52.69%), Query Frame = 1

Query: 76  LLHTLALKCGFKGDVFVGNALVTMYWRWEHLVDARKVFDDMPSRDRV-LWSAMITDYAQE 135
           L+H   L  G + DV +  +L+ +Y+  +    AR VF++   R  V +W+++++ Y++ 
Sbjct: 25  LVHQRILTLGLRRDVVLCKSLINVYFTCKDHCSARHVFENFDIRSDVYIWNSLMSGYSKN 84

Query: 136 -VNNGLQAIFVFVQNVMEYKPNEYTFGSVLNAISA-GEEDIPLKHGQRCHSHLIKVGLNF 195
            + +    +F  + N     P+ +TF +V+ A  A G E +    G+  H+ ++K G   
Sbjct: 85  SMFHDTLEVFKRLLNCSICVPDSFTFPNVIKAYGALGREFL----GRMIHTLVVKSGYVC 144

Query: 196 DPIISGALLDMYAKRGSIQESQRVFNKTSKQSQLAWTVLIFDYAQHG 240
           D +++ +L+ MYAK    + S +VF++  ++   +W  +I  + Q G
Sbjct: 145 DVVVASSLVGMYAKFNLFENSLQVFDEMPERDVASWNTVISCFYQSG 187

HSP 5 Score: 57.0 bits (136), Expect = 2.1e-08
Identity = 58/234 (24.79%), Postives = 105/234 (44.87%), Query Frame = 1

Query: 1   MYCKSGLLERAFSVFENLHDPDIVSWNTILSGFEKSEN---ALSFARRINLNGVEFDSVT 60
           +Y K G    A +VF         SWN ++S +    N   A+    ++   GV+ D VT
Sbjct: 353 LYFKCGEANLAETVFSKTQKDVAESWNVMISSYISVGNWFKAVEVYDQMVSVGVKPDVVT 412

Query: 61  YSTAFSFCFDGKNFLFSWLLHTLALKCGFKGDVFVGNALVTMYWRWEHLVDARKVFDDMP 120
           +++    C           +H    +   + D  + +AL+ MY +  +  +A ++F+ +P
Sbjct: 413 FTSVLPACSQLAALEKGKQIHLSISESRLETDELLLSALLDMYSKCGNEKEAFRIFNSIP 472

Query: 121 SRDRVLWSAMITDYAQEVNNGLQAIFVFVQ-NVMEYKPNEYTFGSVLNAIS-AGEEDIPL 180
            +D V W+ MI+ Y        +A++ F +      KP+  T  +VL+A   AG  D  L
Sbjct: 473 KKDVVSWTVMISAYGSH-GQPREALYQFDEMQKFGLKPDGVTLLAVLSACGHAGLIDEGL 532

Query: 181 KHGQRCHSHLIKVGLNFDPIIS--GALLDMYAKRGSIQESQRVFNKTSKQSQLA 228
           K   +  S   K G+  +PII     ++D+  + G + E+  +  +T + S  A
Sbjct: 533 KFFSQMRS---KYGI--EPIIEHYSCMIDILGRAGRLLEAYEIIQQTPETSDNA 580

BLAST of Cucsa.292810 vs. NCBI nr
Match: gi|778660465|ref|XP_004150024.2| (PREDICTED: LOW QUALITY PROTEIN: pentatricopeptide repeat-containing protein At4g32430, mitochondrial-like [Cucumis sativus])

HSP 1 Score: 290.8 bits (743), Expect = 2.4e-75
Identity = 147/237 (62.03%), Postives = 178/237 (75.11%), Query Frame = 1

Query: 1   MYCKSGLLERAFSVFENLHDPDIVSWNTILSGFEKSENALSFARRINLNGVEFDSVTYST 60
           MYCKSG LERAFSVF+NLHDPDIVSWNTILSGFEKSENALSFA R+NLNGV+FDSVTY+T
Sbjct: 117 MYCKSGQLERAFSVFQNLHDPDIVSWNTILSGFEKSENALSFALRMNLNGVKFDSVTYTT 176

Query: 61  AFSFCFDGKNFLFSWLLHTLALKCGFKGDVFVGNALVTMYWRWEHLVDARKVFDDMPSRD 120
           A SFC DG+ FLF W LHTLALKCGFKGDVFVGNALVTMY RWEHLVDARKVFD+MPSRD
Sbjct: 177 ALSFCLDGEEFLFGWQLHTLALKCGFKGDVFVGNALVTMYSRWEHLVDARKVFDEMPSRD 236

Query: 121 RVLWSAMITDYAQEVNNGLQAIFVFVQNVMEYKPNEYTFGSVLNAISAGEEDIPLKHGQR 180
           RV WSAMIT YAQE +NGLQAIFVFVQN +     ++    +  A+S    +  L+ G++
Sbjct: 237 RVSWSAMITGYAQEGDNGLQAIFVFVQNGV--XGVKFDNVPITGALSVCGHERNLELGKQ 296

Query: 181 CHSHLIKVGLNFDPIISGALLDMYAKRGSIQESQRVFNKTSKQSQLAWTVLIFDYAQ 238
            H   +K G      +   L+  Y+K   I++++ VF   + ++ ++WT +I  Y +
Sbjct: 297 IHCVAVKTGHETHTSVGNVLISTYSKCEIIEDAKAVFELINDRNVISWTTMISLYEE 351

BLAST of Cucsa.292810 vs. NCBI nr
Match: gi|778660465|ref|XP_004150024.2| (PREDICTED: LOW QUALITY PROTEIN: pentatricopeptide repeat-containing protein At4g32430, mitochondrial-like [Cucumis sativus])

HSP 1 Score: 242.7 bits (618), Expect = 7.5e-61
Identity = 133/252 (52.78%), Postives = 177/252 (70.24%), Query Frame = 1

Query: 2   YCKSGLLERAFSVFENLHDPDIVSWNTILSGFEKSENALSFARRINLNGVEFDSVTYSTA 61
           Y K  ++E A +VFE ++D +++SW T++S +E  E A+S   ++ L+GV  + VT+   
Sbjct: 318 YSKCEIIEDAKAVFELINDRNVISWTTMISLYE--EGAVSLFNKMRLDGVYPNDVTF-IG 377

Query: 62  FSFCFDGKNFLFSWLL-HTLALKCGFKGDVFVGNALVTMYWRWEHLVDARKVFDDMPSRD 121
                  +N +   L+ H L +K  F  ++ VGN+L+TMY ++E + DA +VF ++P R+
Sbjct: 378 LLHAITIRNMVEQGLMVHGLCIKADFVSELSVGNSLITMYAKFEFMQDASRVFIELPYRE 437

Query: 122 RVLWSAMITDYAQEVNNGL--QAIFVFVQNVMEYKPNEYTFGSVLNAISAGEEDIPLKHG 181
            + W+A+I+ YAQ   N L  +A+  F+  +MEYKPNEYTFGSVLNAISAGE DI LKHG
Sbjct: 438 IISWNALISGYAQ---NALCQEALEAFLYAIMEYKPNEYTFGSVLNAISAGE-DISLKHG 497

Query: 182 QRCHSHLIKVGLNFDPIISGALLDMYAKRGSIQESQRVFNKTSKQSQLAWTVLIFDYAQH 241
           QRCHSHLIKVGLN DPIISGALLDMYAKRGSIQESQRVFN+TSK+SQ AWT LI  YAQH
Sbjct: 498 QRCHSHLIKVGLNVDPIISGALLDMYAKRGSIQESQRVFNETSKESQFAWTALISGYAQH 557

Query: 242 GGLYFYVSFIEK 251
           G     +   E+
Sbjct: 558 GDYESVIKLFEE 562

HSP 2 Score: 57.8 bits (138), Expect = 3.4e-05
Identity = 52/227 (22.91%), Postives = 103/227 (45.37%), Query Frame = 1

Query: 1   MYCKSGLLERAFSVFENLHDPDIVSWNTILSGFEKSENALSFARRINLNGVEF--DSVTY 60
           MY K   ++ A  VF  L   +I+SWN ++SG+ ++              +E+  +  T+
Sbjct: 413 MYAKFEFMQDASRVFIELPYREIISWNALISGYAQNALCQEALEAFLYAIMEYKPNEYTF 472

Query: 61  STAFSFCFDGKNFLF--SWLLHTLALKCGFKGDVFVGNALVTMYWRWEHLVDARKVFDDM 120
            +  +    G++         H+  +K G   D  +  AL+ MY +   + ++++VF++ 
Sbjct: 473 GSVLNAISAGEDISLKHGQRCHSHLIKVGLNVDPIISGALLDMYAKRGSIQESQRVFNET 532

Query: 121 PSRDRVLWSAMITDYAQEVNNGLQAIFVFVQNVME-YKPNEYTFGSVLNAISAGEEDIPL 180
               +  W+A+I+ YAQ   +    I +F +   E  KP+   F SVL A S       +
Sbjct: 533 SKESQFAWTALISGYAQH-GDYESVIKLFEEMEKERIKPDAVIFLSVLTACSRNR---MV 592

Query: 181 KHGQRCHSHLIKVGLNFDPIIS------GALLDMYAKRGSIQESQRV 217
             G++  + +IK     D +I         ++DM  + G ++E++ +
Sbjct: 593 DMGRQFFNMMIK-----DHMIEPEGEHYSCMVDMLGRAGRLEEAEEI 630

HSP 3 Score: 279.3 bits (713), Expect = 7.3e-72
Identity = 144/237 (60.76%), Postives = 168/237 (70.89%), Query Frame = 1

Query: 1   MYCKSGLLERAFSVFENLHDPDIVSWNTILSGFEKSENALSFARRINLNGVEFDSVTYST 60
           MYCKSG LERAFSVF+NLHDPDIVSWNTILSGFEKSENALSFA R+NLNGV+FDSVTY+T
Sbjct: 117 MYCKSGQLERAFSVFQNLHDPDIVSWNTILSGFEKSENALSFALRMNLNGVKFDSVTYTT 176

Query: 61  AFSFCFDGKNFLFSWLLHTLALKCGFKGDVFVGNALVTMYWRWEHLVDARKVFDDMPSRD 120
           A SFC DG+ FLF W LHTLALKCGFKGDVFVGNALVTMY RWEHLVDARKVFD+MPSRD
Sbjct: 177 ALSFCLDGEEFLFGWQLHTLALKCGFKGDVFVGNALVTMYSRWEHLVDARKVFDEMPSRD 236

Query: 121 RVLWSAMITDYAQEVNNGLQAIFVFVQNVMEYKPNEYTFGSVLNAISAGEEDIPLKHGQR 180
           RV WSAMIT YAQE +NGLQAIFVFVQN                    G   + +K G  
Sbjct: 237 RVSWSAMITGYAQEGDNGLQAIFVFVQN--------------------GIHCVAVKTGHE 296

Query: 181 CHSHLIKVGLNFDPIISGALLDMYAKRGSIQESQRVFNKTSKQSQLAWTVLIFDYAQ 238
            H+            +   L+  Y+K   I++++ VF   + ++ ++WT +I  Y +
Sbjct: 297 THTS-----------VGNVLISTYSKCEIIEDAKAVFELINDRNVISWTTMISLYEE 322

BLAST of Cucsa.292810 vs. NCBI nr
Match: gi|700210223|gb|KGN65319.1| (hypothetical protein Csa_1G320370 [Cucumis sativus])

HSP 1 Score: 233.0 bits (593), Expect = 6.0e-58
Identity = 131/254 (51.57%), Postives = 173/254 (68.11%), Query Frame = 1

Query: 2   YCKSGLLERAFSVFENLHDPDIVSWNTILSGFEKSENALSFARRINLNGVEFDSVTYSTA 61
           Y K  ++E A +VFE ++D +++SW T++S +E  E A+S   ++ L+GV  + VT    
Sbjct: 289 YSKCEIIEDAKAVFELINDRNVISWTTMISLYE--EGAVSLFNKMRLDGVYPNDVT---- 348

Query: 62  FSFCFDGKNFLFSWLLHTLALKCGFKGDVF---VGNALVTMYWRWEHLVDARKVFDDMPS 121
                      F  LLH + ++   +  +    VGN+L+TMY ++E + DA +VF ++P 
Sbjct: 349 -----------FIGLLHAITIRNMVEQGLMNLSVGNSLITMYAKFEFMQDASRVFIELPY 408

Query: 122 RDRVLWSAMITDYAQEVNNGL--QAIFVFVQNVMEYKPNEYTFGSVLNAISAGEEDIPLK 181
           R+ + W+A+I+ YAQ   N L  +A+  F+  +MEYKPNEYTFGSVLNAISAGE DI LK
Sbjct: 409 REIISWNALISGYAQ---NALCQEALEAFLYAIMEYKPNEYTFGSVLNAISAGE-DISLK 468

Query: 182 HGQRCHSHLIKVGLNFDPIISGALLDMYAKRGSIQESQRVFNKTSKQSQLAWTVLIFDYA 241
           HGQRCHSHLIKVGLN DPIISGALLDMYAKRGSIQESQRVFN+TSK+SQ AWT LI  YA
Sbjct: 469 HGQRCHSHLIKVGLNVDPIISGALLDMYAKRGSIQESQRVFNETSKESQFAWTALISGYA 521

Query: 242 QHGGLYFYVSFIEK 251
           QHG     +   E+
Sbjct: 529 QHGDYESVIKLFEE 521

HSP 2 Score: 57.8 bits (138), Expect = 3.4e-05
Identity = 52/227 (22.91%), Postives = 103/227 (45.37%), Query Frame = 1

Query: 1   MYCKSGLLERAFSVFENLHDPDIVSWNTILSGFEKSENALSFARRINLNGVEF--DSVTY 60
           MY K   ++ A  VF  L   +I+SWN ++SG+ ++              +E+  +  T+
Sbjct: 372 MYAKFEFMQDASRVFIELPYREIISWNALISGYAQNALCQEALEAFLYAIMEYKPNEYTF 431

Query: 61  STAFSFCFDGKNFLF--SWLLHTLALKCGFKGDVFVGNALVTMYWRWEHLVDARKVFDDM 120
            +  +    G++         H+  +K G   D  +  AL+ MY +   + ++++VF++ 
Sbjct: 432 GSVLNAISAGEDISLKHGQRCHSHLIKVGLNVDPIISGALLDMYAKRGSIQESQRVFNET 491

Query: 121 PSRDRVLWSAMITDYAQEVNNGLQAIFVFVQNVME-YKPNEYTFGSVLNAISAGEEDIPL 180
               +  W+A+I+ YAQ   +    I +F +   E  KP+   F SVL A S       +
Sbjct: 492 SKESQFAWTALISGYAQH-GDYESVIKLFEEMEKERIKPDAVIFLSVLTACSRNR---MV 551

Query: 181 KHGQRCHSHLIKVGLNFDPIIS------GALLDMYAKRGSIQESQRV 217
             G++  + +IK     D +I         ++DM  + G ++E++ +
Sbjct: 552 DMGRQFFNMMIK-----DHMIEPEGEHYSCMVDMLGRAGRLEEAEEI 589

HSP 3 Score: 274.6 bits (701), Expect = 1.8e-70
Identity = 142/237 (59.92%), Postives = 174/237 (73.42%), Query Frame = 1

Query: 1   MYCKSGLLERAFSVFENLHDPDIVSWNTILSGFEKSENALSFARRINLNGVEFDSVTYST 60
           MYCKSG LERAFSVFENLHDPDIVSWNTILSGFEKSENALSFA R+NLNGV+FDSVTY+T
Sbjct: 122 MYCKSGQLERAFSVFENLHDPDIVSWNTILSGFEKSENALSFALRMNLNGVKFDSVTYTT 181

Query: 61  AFSFCFDGKNFLFSWLLHTLALKCGFKGDVFVGNALVTMYWRWEHLVDARKVFDDMPSRD 120
           A SFC D ++FLF W LH+LALKCG + DVFVGNALVTMY R EHLVDARKVFD+MPSRD
Sbjct: 182 ALSFCLDIEDFLFGWQLHSLALKCGLESDVFVGNALVTMYSRCEHLVDARKVFDEMPSRD 241

Query: 121 RVLWSAMITDYAQEVNNGLQAIFVFVQNVMEYKPNEYTFGSVLNAISAGEEDIPLKHGQR 180
           RV WSAMIT YAQE +NGLQAI VFVQ V E    ++    +  A+S    +  L+ G++
Sbjct: 242 RVSWSAMITGYAQEGDNGLQAILVFVQMVRE--GVKFDNVPITGALSVCGHERNLELGKQ 301

Query: 181 CHSHLIKVGLNFDPIISGALLDMYAKRGSIQESQRVFNKTSKQSQLAWTVLIFDYAQ 238
            H   +K G      +   L+  Y+K   I++++ VF   + ++ ++WT +I  Y +
Sbjct: 302 IHCLAVKTGHETHTSVGNVLISTYSKCEIIEDAKAVFELINDRNVISWTTMISLYEE 356

BLAST of Cucsa.292810 vs. NCBI nr
Match: gi|659086583|ref|XP_008444010.1| (PREDICTED: pentatricopeptide repeat-containing protein At4g32430, mitochondrial [Cucumis melo])

HSP 1 Score: 245.7 bits (626), Expect = 8.9e-62
Identity = 135/252 (53.57%), Postives = 177/252 (70.24%), Query Frame = 1

Query: 2   YCKSGLLERAFSVFENLHDPDIVSWNTILSGFEKSENALSFARRINLNGVEFDSVTYSTA 61
           Y K  ++E A +VFE ++D +++SW T++S +E  E A+S   ++ L+GV  + VT+   
Sbjct: 323 YSKCEIIEDAKAVFELINDRNVISWTTMISLYE--EGAVSLFNKMRLDGVYPNDVTF-IG 382

Query: 62  FSFCFDGKNFLFSWLL-HTLALKCGFKGDVFVGNALVTMYWRWEHLVDARKVFDDMPSRD 121
                  +N +   L+ H L +K  F  ++ VGN+L+TMY ++E + DA +VF ++P R+
Sbjct: 383 LLHAITIRNMVEQGLMVHGLCIKADFVSELTVGNSLITMYAKFEFMQDASRVFMELPYRE 442

Query: 122 RVLWSAMITDYAQEVNNGL--QAIFVFVQNVMEYKPNEYTFGSVLNAISAGEEDIPLKHG 181
            + W+A+I+ YAQ   N L  +A+  F   +MEYKPNEYTFGSVLNAISAGE DI LKHG
Sbjct: 443 IISWNALISGYAQ---NALCQEALEAFFYAIMEYKPNEYTFGSVLNAISAGE-DISLKHG 502

Query: 182 QRCHSHLIKVGLNFDPIISGALLDMYAKRGSIQESQRVFNKTSKQSQLAWTVLIFDYAQH 241
           QRCHSHLIKVGLNFDPIISGALLDMYAKRGSIQESQRVFN+TSKQSQ AWT LI  YAQH
Sbjct: 503 QRCHSHLIKVGLNFDPIISGALLDMYAKRGSIQESQRVFNETSKQSQFAWTALISGYAQH 562

Query: 242 GGLYFYVSFIEK 251
           G     +   E+
Sbjct: 563 GDYESVIKLFEE 567

HSP 2 Score: 58.9 bits (141), Expect = 1.5e-05
Identity = 52/227 (22.91%), Postives = 103/227 (45.37%), Query Frame = 1

Query: 1   MYCKSGLLERAFSVFENLHDPDIVSWNTILSGFEKSENALSFARRINLNGVEF--DSVTY 60
           MY K   ++ A  VF  L   +I+SWN ++SG+ ++              +E+  +  T+
Sbjct: 418 MYAKFEFMQDASRVFMELPYREIISWNALISGYAQNALCQEALEAFFYAIMEYKPNEYTF 477

Query: 61  STAFSFCFDGKNFLF--SWLLHTLALKCGFKGDVFVGNALVTMYWRWEHLVDARKVFDDM 120
            +  +    G++         H+  +K G   D  +  AL+ MY +   + ++++VF++ 
Sbjct: 478 GSVLNAISAGEDISLKHGQRCHSHLIKVGLNFDPIISGALLDMYAKRGSIQESQRVFNET 537

Query: 121 PSRDRVLWSAMITDYAQEVNNGLQAIFVFVQNVME-YKPNEYTFGSVLNAISAGEEDIPL 180
             + +  W+A+I+ YAQ   +    I +F +   E  KP+   F SVL A S       +
Sbjct: 538 SKQSQFAWTALISGYAQH-GDYESVIKLFEEMEKEKIKPDAVIFLSVLTACSRNR---MV 597

Query: 181 KHGQRCHSHLIKVGLNFDPIIS------GALLDMYAKRGSIQESQRV 217
             G++    +IK     D +I         ++DM  + G ++E++ +
Sbjct: 598 NMGRQLFDMMIK-----DHMIEPEGEHYSCMVDMLGRAGRLEEAEEI 635

HSP 3 Score: 211.8 bits (538), Expect = 1.4e-51
Identity = 105/233 (45.06%), Postives = 161/233 (69.10%), Query Frame = 1

Query: 1   MYCKSGLLERAFSVFENLHDPDIVSWNTILSGFEKSENALSFARRINLNGVEFDSVTYST 60
           MYCK+GL +RA  VFENL++PDIVSWNT+LSGF++S++AL+FA R+N  GV FD+VT +T
Sbjct: 122 MYCKAGLFDRALVVFENLNNPDIVSWNTVLSGFQRSDDALNFALRMNFTGVAFDAVTCTT 181

Query: 61  AFSFCFDGKNFLFSWLLHTLALKCGFKGDVFVGNALVTMYWRWEHLVDARKVFDDMPSRD 120
             +FC D + F+F + LH+  LKCG   +VFVGNAL+TMY R   LV+AR+VFD+M ++D
Sbjct: 182 VLAFCSDHEGFIFGFQLHSRILKCGLDCEVFVGNALITMYSRCCRLVEARRVFDEMRNKD 241

Query: 121 RVLWSAMITDYAQEVNNGLQAIFVFVQNVME-YKPNEYTFGSVLNAISAGEEDIPLKHGQ 180
            V W+AM++ YAQE N+GL+AI VF++ + E  K +  +F   ++A   G+E    + G+
Sbjct: 242 LVSWNAMLSGYAQEGNSGLEAILVFLEMLKEGMKLDHVSFTGAISACGHGKE---FELGR 301

Query: 181 RCHSHLIKVGLNFDPIISGALLDMYAKRGSIQESQRVFNKTSKQSQLAWTVLI 233
           + HS  +K+G +    +   L+  Y+K   I++++ VF     ++ ++WT +I
Sbjct: 302 QIHSLAVKIGYDTHVKVCNVLISTYSKCEDIEDAKLVFESIIDRNVVSWTTMI 351

BLAST of Cucsa.292810 vs. NCBI nr
Match: gi|225445472|ref|XP_002281953.1| (PREDICTED: pentatricopeptide repeat-containing protein At4g32430, mitochondrial [Vitis vinifera])

HSP 1 Score: 181.4 bits (459), Expect = 2.1e-42
Identity = 101/241 (41.91%), Postives = 161/241 (66.80%), Query Frame = 1

Query: 2   YCKSGLLERAFSVFENLHDPDIVSWNTILSGFEKSENALSFARRINLNGVEFDSVTYSTA 61
           Y K   +E A  VFE++ D ++VSW T++S  E  E+A S    +  +GV  + VT+   
Sbjct: 323 YSKCEDIEDAKLVFESIIDRNVVSWTTMISISE--EDATSLFNEMRRDGVYPNDVTF-VG 382

Query: 62  FSFCFDGKNFLFSW-LLHTLALKCGFKGDVFVGNALVTMYWRWEHLVDARKVFDDMPSRD 121
                  KN +    ++H + +K  F  ++ V N+L+TMY ++E + D+ KVF+++  R+
Sbjct: 383 LIHAITMKNLVEEGQMIHGVCVKTSFLSELNVSNSLITMYAKFESMSDSMKVFEELNYRE 442

Query: 122 RVLWSAMITDYAQEVNNGL--QAIFVFVQNVMEYKPNEYTFGSVLNAISAGEEDIPLKHG 181
            + W+++I+ YAQ   NGL  +A+  F+  +ME +PNE+TFGSVL++I++ E  I ++HG
Sbjct: 443 IISWNSLISGYAQ---NGLWQEALQTFLSALMESRPNEFTFGSVLSSIASAEA-ISMRHG 502

Query: 182 QRCHSHLIKVGLNFDPIISGALLDMYAKRGSIQESQRVFNKTSKQSQLAWTVLIFDYAQH 240
           QRCHSH++K+GLN +PI+S ALLDMYAKRGSI ES  VF++T  ++++AWT +I  +A+H
Sbjct: 503 QRCHSHILKLGLNTNPIVSSALLDMYAKRGSICESLGVFSETPLKNEVAWTAIISAHARH 556

HSP 2 Score: 63.2 bits (152), Expect = 8.2e-07
Identity = 55/224 (24.55%), Postives = 108/224 (48.21%), Query Frame = 1

Query: 1   MYCKSGLLERAFSVFENLHDPDIVSWNTILSGF-------EKSENALSFARRINLNGVEF 60
           MY K   +  +  VFE L+  +I+SWN+++SG+       E  +  LS       N   F
Sbjct: 418 MYAKFESMSDSMKVFEELNYREIISWNSLISGYAQNGLWQEALQTFLSALMESRPNEFTF 477

Query: 61  DSVTYSTAFSFCFDGKNFLFSWLLHTLALKCGFKGDVFVGNALVTMYWRWEHLVDARKVF 120
            SV  S A +   +  +       H+  LK G   +  V +AL+ MY +   + ++  VF
Sbjct: 478 GSVLSSIASA---EAISMRHGQRCHSHILKLGLNTNPIVSSALLDMYAKRGSICESLGVF 537

Query: 121 DDMPSRDRVLWSAMITDYAQEVNNGLQAIFVFVQNVME--YKPNEYTFGSVLNAISAGEE 180
            + P ++ V W+A+I+ +A+  +   +A+    +++     KP+  TF +V+ A   G +
Sbjct: 538 SETPLKNEVAWTAIISAHAR--HGDYEAVMNLFKDMEREGVKPDSITFLAVITA--CGRK 597

Query: 181 DIPLKHGQRCHSHLIKVGL-NFDPIISGALLDMYAKRGSIQESQ 215
            + +  G +  + ++K  L    P    +++DM  + G ++E++
Sbjct: 598 GM-VDTGYQLFNSMVKDHLIEPSPEHYSSMVDMLGRAGRLKEAE 633

HSP 3 Score: 207.6 bits (527), Expect = 2.7e-50
Identity = 104/232 (44.83%), Postives = 153/232 (65.95%), Query Frame = 1

Query: 1   MYCKSGLLERAFSVFENLHDPDIVSWNTILSGFEKSENALSFARRINLNGVEFDSVTYST 60
           MYCKSG  ++A  +F NL++PDIVSWNT+LSGFEKS++ALSFA R+NL GV FD+VTYST
Sbjct: 3   MYCKSGQFDKALCIFNNLNNPDIVSWNTVLSGFEKSDDALSFALRMNLIGVVFDAVTYST 62

Query: 61  AFSFCFDGKNFLFSWLLHTLALKCGFKGDVFVGNALVTMYWRWEHLVDARKVFDDMPSRD 120
           A SFC D + FLF   LH+L +K G   +V+VGNAL+TMY RW  LV+AR+VFD+MP++D
Sbjct: 63  ALSFCLDHEGFLFGLQLHSLIVKFGLDSEVYVGNALITMYSRWGRLVEARRVFDEMPNKD 122

Query: 121 RVLWSAMITDYAQEVNNGLQAIFVFVQNVMEYKPNEYTFGSVLNAISAGEEDIPLKHGQR 180
            V W+A+++ Y Q+ + G++AI   ++  M  K       S  +A SA   +  L+ G++
Sbjct: 123 SVSWNAILSGYTQDGDYGVEAILALIE--MMRKGLRLDHVSFTSAASACGHEKNLELGKQ 182

Query: 181 CHSHLIKVGLNFDPIISGALLDMYAKRGSIQESQRVFNKTSKQSQLAWTVLI 233
            H   IK+G      +   L+  Y+K     ++ +VF +   ++ ++WT +I
Sbjct: 183 IHGVSIKMGYGTHVSVGNVLMSTYSKCEVTGDANKVFRRMHDRNVISWTTMI 232