Cucsa.291730 (gene) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.291730
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	Pseudouridine synthase family protein
Location	scaffold02766 : 294676 .. 295757 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAAGAGCTTCAAGGGACATGAGAAATTTCAATATATTCATTAAGGAAAATAACTTCATGGAAGTTCCTCTTTCAAACGGCCGATTCACTTGGTCAAGTGAAGGAGAAGTAATCTCTAAATCTCTTATTGACAGATTTCTGGTTTCTAACAAGTGGGAGGAAGCTTTTGAGAAGAGGAAGGCATTCAGTACCTCCTCTTTGAGTGCAGTTATGCTCAAAATTGTTGGTTTGCATTATTTAAATTTTTTGGCTTGGGATAGAAGATATGTTCAAGTGTTTTTTtGATAGATTTGATCAACCCCAAATTAGATTTTTGTACTCATGATGGTGCAAATGAGAGCCAATGCAGATAAAAAATTACACTGCATCTGAATCAGCAATATATTTTGTTAATTGATGCAATGGGTGCTATTGATTCAGTATTTCTAAATGTTTTGCTAACTATGCCATCTGTGTTTTTGAATTAAGCGTTTTGGCAGTGGTTCTGTACCGACTCATCTTGTTGGAGCTTCACTTCTTAGAGGAGAGTGGAAGGGTGCTGTCAATATGATTCTTGATCCAAGAGAAGGGGATATCCTTTTATTAATGCGTGTGCAAGGAAGGATTGTCACTCTACTTATCTGCTTGCTTTCCAATGTTTTTCTCCTTGCAGGCCTAGTTTGTTTCCTTAACTCAATTTTTAATTACAAACCAATGAAATTGCAAAGGCAAGAGACTACTACAAGGAAAGTGATGATATTGATGGAACGTTGAAGCAATTACCTCGATATTTAGTGGCTGAAAGAGCAATAGTAAGTTCATAGATATAATAGTCAGTCATTAATTCCACATTTTTGATTTTCTGATACAGTTATCTCGTAAATGATTTTtTTTtATTACTGTAGTACTGATTGCCTGCAACGTACATACATACATGGATGTGTGTGATTTTGCATTATAGCAAGCACACTGTATTTTCCTTGTGTGTAATAATGATGTAATTCTTGCTCTTTCTAAATTATGCAGCTACAATGTTTGAAGAAATGTCCTGGGAACTATTTGCAGGCTCTTAAAGCTATACCTAGAACTTTGAGAATGAT

mRNA sequence

atgggaagagcttcaagggacatgagaaatttcaatatattcattaaggaaaataacttcatggaagttcctctttcaaacggccgattcacttggtcaagtgaaggagaagtaatctctaaatctcttattgacagatttctggtttctaacaagtgggaggaagcttttgagaaGAGGAAGGCATTCAGTACCTCCTCTTTGAGTGCAGTTATGCTCAAAATTGTTGGTTTGCATTATTTAAATTTTTTGGCTTGGGATAGAAGATATGTTCAACGTTTTGGCAGTGGTTCTGTACCGACTCATCTTGTTGGAGCTTCACTTCTTAGAGGAGAGTGGAAGGGTGCTGTCAATATGATTCTTGATCCAAGAGAAGGGGATATCCTTTTATTAATGCGTGCAAGAGACTACTACAAGGAAAGTGATGATATTGATGGAACGTTGAAGCAATTACCTCGATATTTAGTGGCTGAAAGAGCAATACTACAATGTTTGAAGAAATGTCCTGGGAACTATTTGCAGGCTCTTAAAGCTATACCTAGAACTTTGAGAATGAT

Coding sequence (CDS)

ATGGGAAGAGCTTCAAGGGACATGAGAAATTTCAATATATTCATTAAGGAAAATAACTTCATGGAAGTTCCTCTTTCAAACGGCCGATTCACTTGGTCAAGTGAAGGAGAAGTAATCTCTAAATCTCTTATTGACAGATTTCTGGTTTCTAACAAGTGGGAGGAAGCTTTTGAGAAGAGGAAGGCATTCAGTACCTCCTCTTTGAGTGCAGTTATGCTCAAAATTGTTGGTTTGCATTATTTAAATTTTTTGGCTTGGGATAGAAGATATGTTCAACGTTTTGGCAGTGGTTCTGTACCGACTCATCTTGTTGGAGCTTCACTTCTTAGAGGAGAGTGGAAGGGTGCTGTCAATATGATTCTTGATCCAAGAGAAGGGGATATCCTTTTATTAATGCGTGCAAGAGACTACTACAAGGAAAGTGATGATATTGATGGAACGTTGAAGCAATTACCTCGATATTTAGTGGCTGAAAGAGCAATACTACAATGTTTGAAGAAATGTCCTGGGAACTATTTGCAGGCTCTTAAAGCTATACCTAGAACTTTGAGAATGAT

Protein sequence

MGRASRDMRNFNIFIKENNFMEVPLSNGRFTWSSEGEVISKSLIDRFLVSNKWEEAFEKRKAFSTSSLSAVMLKIVGLHYLNFLAWDRRYVQRFGSGSVPTHLVGASLLRGEWKGAVNMILDPREGDILLLMRARDYYKESDDIDGTLKQLPRYLVAERAILQCLKKCPGNYLQALKAIPRTLRMX

Homology

[Close]

BLAST of Cucsa.291730 vs. Swiss-Prot
Match: YH2X_SCHPO (Putative pseudouridine synthase C1A4.09 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=SPBC1A4.09 PE=3 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 2.6e-12
Identity = 39/96 (40.62%), Postives = 59/96 (61.46%), Query Frame = 1


Query: 91  VQRFGSGSVPTHLVGASLLRGEWKGAVNMILDPREGDILLLMRARDYYKESDDIDGTLKQ 150
           +QRFG+ SV TH +G  LL+ +WKGAV++IL PR      +  A D +  + D + +L+ 
Sbjct: 365 LQRFGTSSVGTHTIGVRLLQSDWKGAVDLILSPRPEHTGSVKEAIDLWHSTHDAEASLRI 424

Query: 151 LPRYLVAERAILQCLKKCPG--NYLQALKAIPRTLR 185
           LPR ++AE +IL+   +     +YL A + IPR LR
Sbjct: 425 LPRRMIAESSILETWSRSGNQTDYLGAFQRIPRHLR 460

[Close]

BLAST of Cucsa.291730 vs. Swiss-Prot
Match: PUS7_YEAST (Multisubstrate pseudouridine synthase 7 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PUS7 PE=1 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 1.8e-08
Identity = 38/103 (36.89%), Postives = 54/103 (52.43%), Query Frame = 1


Query: 91  VQRFGSGSVPTHLVGASLLRGEWKGAVNMILDPREGDILLLMRARDYYKESDDIDGTLKQ 150
           +QRFG+ S+ TH +G  LL   WK A  +IL  ++  +     AR  + E+ D    LKQ
Sbjct: 345 MQRFGTFSISTHTIGRELLLSNWKKAAELILSDQDNVLPKSKEARKIWAETKDAALALKQ 404

Query: 151 LPRYLVAERAILQCL----KKCPGNYLQ-----ALKAIPRTLR 185
           +PR  +AE A+L  L    K+  G Y +     A+  IPR LR
Sbjct: 405 MPRQCLAENALLYSLSNQRKEEDGTYSENAYYTAIMKIPRNLR 447

[Close]

BLAST of Cucsa.291730 vs. Swiss-Prot
Match: TRUD_HALMA (Probable tRNA pseudouridine synthase D OS=Haloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809) GN=truD PE=3 SV=1)

HSP 1 Score: 56.6 bits (135), Expect = 3.3e-07
Identity = 38/98 (38.78%), Postives = 53/98 (54.08%), Query Frame = 1


Query: 92  QRFGSGSVPTHLVGASLLRGEWKGAV-NMILDPREGDILLLMRARDYYKESDDIDGTLKQ 151
           QRFGS    TH VG ++ RGEWK AV   + +P E +      AR Y  E+ D  G L +
Sbjct: 197 QRFGSRRPVTHEVGLAIARGEWKDAVLAYVGNPNEREPESTQEARGYVDETHDWAGALDR 256

Query: 152 LPRYLVAERAILQCLKKC----PGNYLQALKAIPRTLR 185
           LP  L  ER+I   L +     P ++ +AL+A+P  L+
Sbjct: 257 LPGALGYERSICHRLVENGASEPADFREALEALPSNLQ 294

[Close]

BLAST of Cucsa.291730 vs. Swiss-Prot
Match: PUS7L_HUMAN (Pseudouridylate synthase 7 homolog-like protein OS=Homo sapiens GN=PUS7L PE=1 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 1.7e-06
Identity = 33/98 (33.67%), Postives = 56/98 (57.14%), Query Frame = 1


Query: 92  QRFGSG-SVPTHLVGASLLRGEWKGAVNMILDPREGDILLLMRARDYYKESDDIDGTLKQ 151
           QRFG G  V T  +G +LL+ E   A+ + L P + D  +  RA+ Y+ +++D  GTL  
Sbjct: 432 QRFGKGRKVHTDQIGLALLKNEMMKAIKLFLTPEDLDDPV-NRAKKYFLQTEDAKGTLSL 491

Query: 152 LPRYLVAERAILQCLKK---CPGNYLQALKAIPRTLRM 186
           +P + V ERA+L+ L +        +QA  ++P ++R+
Sbjct: 492 MPEFKVRERALLEALHRFGMTEEGCIQAWFSLPHSMRI 528

[Close]

BLAST of Cucsa.291730 vs. Swiss-Prot
Match: TRUD_THEGJ (Probable tRNA pseudouridine synthase D OS=Thermococcus gammatolerans (strain DSM 15229 / JCM 11827 / EJ3) GN=truD PE=3 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 2.8e-06
Identity = 31/89 (34.83%), Postives = 52/89 (58.43%), Query Frame = 1


Query: 92  QRFGSGSVPTHLVGASLLRGEWKGAVNMILDPREGDILLLMRARDYYKESDDIDGTLKQL 151
           QRFG   V  HL+G  LL+GE+  A  + L   +G +      R+++ E++D+D  L++ 
Sbjct: 166 QRFGERRVVNHLIGKLLLQGEFDEAARLFLGYADGSMEGDEARRNFW-ETEDVDRALEEF 225

Query: 152 PRYLVAERAILQCLKKCPGNYLQALKAIP 181
           PR+L  ER +L   KK  G++ +A  ++P
Sbjct: 226 PRFLRYERTLLYTYKK-TGSWKKAFLSLP 252

[Close]

BLAST of Cucsa.291730 vs. TrEMBL
Match: A0A0A0LY46_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G690320 PE=4 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 4.1e-44
Identity = 94/99 (94.95%), Postives = 94/99 (94.95%), Query Frame = 1


Query: 92  QRFGSGSVPTHLVGASLLRGEWKGAVNMILDPREGDILLLMR-----ARDYYKESDDIDG 151
           QRFGSGSVPTHLVGASLLRGEWKGAVNMILDPREGDILLLMR     ARDYYKESDDIDG
Sbjct: 192 QRFGSGSVPTHLVGASLLRGEWKGAVNMILDPREGDILLLMRVQGRIARDYYKESDDIDG 251

Query: 152 TLKQLPRYLVAERAILQCLKKCPGNYLQALKAIPRTLRM 186
           TLKQLPRYLVAERAILQCLKKCPGNYLQALKAIPRTLRM
Sbjct: 252 TLKQLPRYLVAERAILQCLKKCPGNYLQALKAIPRTLRM 290

[Close]

BLAST of Cucsa.291730 vs. TrEMBL
Match: B9RER1_RICCO (tRNA pseudouridine synthase d, putative OS=Ricinus communis GN=RCOM_1427850 PE=4 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 3.6e-40
Identity = 100/166 (60.24%), Postives = 120/166 (72.29%), Query Frame = 1


Query: 28  GRFTWSSEGEVISKSLIDRFLVSNKWEEAFEKRKAFSTSSLSAVMLKIVGLH-YLNFLAW 87
           G F + +EG ++ + L +RF V+ +   A  +     T   SA  L   G H ++N+   
Sbjct: 312 GDFCYVNEGLLLGQLLGNRFTVTLRGVVADSE----DTIKGSADSL---GRHGFINYFG- 371

Query: 88  DRRYVQRFGSGSVPTHLVGASLLRGEWKGAVNMILDPREGDILLLM-------RARDYYK 147
               +QRFGSGSVPTHL+GA+LLRGEWK AV+MILDPREGDILLL+        AR+YYK
Sbjct: 372 ----LQRFGSGSVPTHLIGAALLRGEWKYAVSMILDPREGDILLLVICFMWMRMAREYYK 431

Query: 148 ESDDIDGTLKQLPRYLVAERAILQCLKKCPGNYLQALKAIPRTLRM 186
           ESDDIDGTL+QLPRYL AERA+LQCLKKCPGNYLQALKAIPRTLRM
Sbjct: 432 ESDDIDGTLRQLPRYLTAERAVLQCLKKCPGNYLQALKAIPRTLRM 465

[Close]

BLAST of Cucsa.291730 vs. TrEMBL
Match: V4V316_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10003382mg PE=4 SV=1)

HSP 1 Score: 170.2 bits (430), Expect = 2.3e-39
Identity = 83/95 (87.37%), Postives = 91/95 (95.79%), Query Frame = 1


Query: 91  VQRFGSGSVPTHLVGASLLRGEWKGAVNMILDPREGDILLLMRARDYYKESDDIDGTLKQ 150
           +QRFGSGSVPTHLVGA+LLRGEWK AV MILDPREGDILL  +AR+YYKE+DDIDGTL++
Sbjct: 315 LQRFGSGSVPTHLVGATLLRGEWKSAVKMILDPREGDILLF-KAREYYKETDDIDGTLRK 374

Query: 151 LPRYLVAERAILQCLKKCPGNYLQALKAIPRTLRM 186
           LPR+LVAERAILQCLKKCPGNYLQALKAIPRTLRM
Sbjct: 375 LPRHLVAERAILQCLKKCPGNYLQALKAIPRTLRM 408

[Close]

BLAST of Cucsa.291730 vs. TrEMBL
Match: F6HTS9_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_14s0030g01850 PE=4 SV=1)

HSP 1 Score: 167.2 bits (422), Expect = 1.9e-38
Identity = 95/158 (60.13%), Postives = 117/158 (74.05%), Query Frame = 1


Query: 28  GRFTWSSEGEVISKSLIDRFLVSNKWEEAFEKRKAFSTSSLSAVMLKIVGLHYLNFLAWD 87
           G F    EG V+ + L +RF ++ +   A  +     T   SA  L  +G  ++N+    
Sbjct: 86  GDFCHVREGLVLGQLLGNRFTITLRGVVADSEE----TIKASADALGRLG--FINYFG-- 145

Query: 88  RRYVQRFGSGSVPTHLVGASLLRGEWKGAVNMILDPREGDILLLMRARDYYKESDDIDGT 147
              +QRFGSGSVPTHL+GA+LLRGEW+ AV++ILDPREGDILLL +AR+YYKESDDI+GT
Sbjct: 146 ---LQRFGSGSVPTHLIGAALLRGEWETAVSLILDPREGDILLL-KAREYYKESDDIEGT 205

Query: 148 LKQLPRYLVAERAILQCLKKCPGNYLQALKAIPRTLRM 186
           L+Q PR+LVAERAILQCLKK PGNYLQALKAIPRTLRM
Sbjct: 206 LRQFPRHLVAERAILQCLKKYPGNYLQALKAIPRTLRM 231

[Close]

BLAST of Cucsa.291730 vs. TrEMBL
Match: M5WRD4_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa020985mg PE=3 SV=1)

HSP 1 Score: 167.2 bits (422), Expect = 1.9e-38
Identity = 88/160 (55.00%), Postives = 118/160 (73.75%), Query Frame = 1


Query: 28  GRFTWSSEGEVISKSLIDRFLVSNKWEEAFEKRKAFSTSSLSAVMLKIVGLHYLNFLAWD 87
           G F +  EG V+ + L +RF ++ +   A       S  ++ A ++ +    ++N+    
Sbjct: 259 GDFCYVKEGLVLGQLLGNRFTITLRGVVAD------SEDTIKASVIALGKQGFINYFG-- 318

Query: 88  RRYVQRFGSGSVPTHLVGASLLRGEWKGAVNMILDPREGDILLLMR--ARDYYKESDDID 147
              +QRFGSGSVPTHL+GA+LLRGEWK  V++ LDPREGDILL++   AR+YYKE++DI+
Sbjct: 319 ---LQRFGSGSVPTHLIGATLLRGEWKSTVSLFLDPREGDILLMLLGIAREYYKETNDIE 378

Query: 148 GTLKQLPRYLVAERAILQCLKKCPGNYLQALKAIPRTLRM 186
           GTL+QLPR+LV+E+AILQCLKKCPGNYLQALKAIPRTLRM
Sbjct: 379 GTLRQLPRHLVSEKAILQCLKKCPGNYLQALKAIPRTLRM 407

[Close]

BLAST of Cucsa.291730 vs. TAIR10
Match: AT3G04820.1 (AT3G04820.1 Pseudouridine synthase family protein)

HSP 1 Score: 157.1 bits (396), Expect = 1.0e-38
Identity = 76/95 (80.00%), Postives = 86/95 (90.53%), Query Frame = 1


Query: 91  VQRFGSGSVPTHLVGASLLRGEWKGAVNMILDPREGDILLLMRARDYYKESDDIDGTLKQ 150
           +QRFGSGSVPTH VGA+LL+GEWK AV+MILDPRE +  ++  AR+YYKE+ DIDGTL+Q
Sbjct: 349 LQRFGSGSVPTHHVGAALLKGEWKDAVDMILDPRETERHVVNDAREYYKETGDIDGTLRQ 408

Query: 151 LPRYLVAERAILQCLKKCPGNYLQALKAIPRTLRM 186
           LPRYLVAERAILQCLKKCPGNYLQALK IPRTLRM
Sbjct: 409 LPRYLVAERAILQCLKKCPGNYLQALKGIPRTLRM 443

[Close]

BLAST of Cucsa.291730 vs. NCBI nr
Match: gi|700211699|gb|KGN66795.1| (hypothetical protein Csa_1G690320 [Cucumis sativus])

HSP 1 Score: 186.0 bits (471), Expect = 5.8e-44
Identity = 94/99 (94.95%), Postives = 94/99 (94.95%), Query Frame = 1


Query: 92  QRFGSGSVPTHLVGASLLRGEWKGAVNMILDPREGDILLLMR-----ARDYYKESDDIDG 151
           QRFGSGSVPTHLVGASLLRGEWKGAVNMILDPREGDILLLMR     ARDYYKESDDIDG
Sbjct: 192 QRFGSGSVPTHLVGASLLRGEWKGAVNMILDPREGDILLLMRVQGRIARDYYKESDDIDG 251

Query: 152 TLKQLPRYLVAERAILQCLKKCPGNYLQALKAIPRTLRM 186
           TLKQLPRYLVAERAILQCLKKCPGNYLQALKAIPRTLRM
Sbjct: 252 TLKQLPRYLVAERAILQCLKKCPGNYLQALKAIPRTLRM 290

[Close]

BLAST of Cucsa.291730 vs. NCBI nr
Match: gi|778664378|ref|XP_011660284.1| (PREDICTED: pseudouridylate synthase 7 homolog [Cucumis sativus])

HSP 1 Score: 184.9 bits (468), Expect = 1.3e-43
Identity = 98/159 (61.64%), Postives = 119/159 (74.84%), Query Frame = 1


Query: 28  GRFTWSSEGEVISKSLIDRFLVSNKWEEAFEKRKAFSTSSLSAVMLKIVGLH-YLNFLAW 87
           G F++  EG ++ + L +RF ++ +   A       ++  +     + +G H ++N+   
Sbjct: 297 GNFSYIQEGLLLGQLLGNRFTITLRGVVA-------NSEDVIKASAEALGRHGFINYFG- 356

Query: 88  DRRYVQRFGSGSVPTHLVGASLLRGEWKGAVNMILDPREGDILLLMRARDYYKESDDIDG 147
               +QRFGSGSVPTHLVGASLLRGEWKGAVNMILDPREG+   + +ARDYYKESDDIDG
Sbjct: 357 ----LQRFGSGSVPTHLVGASLLRGEWKGAVNMILDPREGETNEIAKARDYYKESDDIDG 416

Query: 148 TLKQLPRYLVAERAILQCLKKCPGNYLQALKAIPRTLRM 186
           TLKQLPRYLVAERAILQCLKKCPGNYLQALKAIPRTLRM
Sbjct: 417 TLKQLPRYLVAERAILQCLKKCPGNYLQALKAIPRTLRM 443

[Close]

BLAST of Cucsa.291730 vs. NCBI nr
Match: gi|659125504|ref|XP_008462716.1| (PREDICTED: pseudouridylate synthase 7 homolog isoform X1 [Cucumis melo])

HSP 1 Score: 183.7 bits (465), Expect = 2.9e-43
Identity = 97/159 (61.01%), Postives = 119/159 (74.84%), Query Frame = 1


Query: 28  GRFTWSSEGEVISKSLIDRFLVSNKWEEAFEKRKAFSTSSLSAVMLKIVGLH-YLNFLAW 87
           G F++  EG ++ + L +RF ++ +   A       ++  +     + +G H ++N+   
Sbjct: 297 GNFSYIQEGLLLGQLLGNRFTITLRGVVA-------NSEDVIKASAEALGTHGFINYFG- 356

Query: 88  DRRYVQRFGSGSVPTHLVGASLLRGEWKGAVNMILDPREGDILLLMRARDYYKESDDIDG 147
               +QRFGSGSVPTHLVGASLLRGEWKGAVNMILDPREG+   + +AR+YYKESDDIDG
Sbjct: 357 ----LQRFGSGSVPTHLVGASLLRGEWKGAVNMILDPREGETNEIAKAREYYKESDDIDG 416

Query: 148 TLKQLPRYLVAERAILQCLKKCPGNYLQALKAIPRTLRM 186
           TLKQLPRYLVAERAILQCLKKCPGNYLQALKAIPRTLRM
Sbjct: 417 TLKQLPRYLVAERAILQCLKKCPGNYLQALKAIPRTLRM 443

[Close]

BLAST of Cucsa.291730 vs. NCBI nr
Match: gi|659125508|ref|XP_008462718.1| (PREDICTED: pseudouridylate synthase 7 homolog isoform X2 [Cucumis melo])

HSP 1 Score: 183.7 bits (465), Expect = 2.9e-43
Identity = 97/159 (61.01%), Postives = 119/159 (74.84%), Query Frame = 1


Query: 28  GRFTWSSEGEVISKSLIDRFLVSNKWEEAFEKRKAFSTSSLSAVMLKIVGLH-YLNFLAW 87
           G F++  EG ++ + L +RF ++ +   A       ++  +     + +G H ++N+   
Sbjct: 267 GNFSYIQEGLLLGQLLGNRFTITLRGVVA-------NSEDVIKASAEALGTHGFINYFG- 326

Query: 88  DRRYVQRFGSGSVPTHLVGASLLRGEWKGAVNMILDPREGDILLLMRARDYYKESDDIDG 147
               +QRFGSGSVPTHLVGASLLRGEWKGAVNMILDPREG+   + +AR+YYKESDDIDG
Sbjct: 327 ----LQRFGSGSVPTHLVGASLLRGEWKGAVNMILDPREGETNEIAKAREYYKESDDIDG 386

Query: 148 TLKQLPRYLVAERAILQCLKKCPGNYLQALKAIPRTLRM 186
           TLKQLPRYLVAERAILQCLKKCPGNYLQALKAIPRTLRM
Sbjct: 387 TLKQLPRYLVAERAILQCLKKCPGNYLQALKAIPRTLRM 413

[Close]

BLAST of Cucsa.291730 vs. NCBI nr
Match: gi|223548191|gb|EEF49682.1| (tRNA pseudouridine synthase d, putative [Ricinus communis])

HSP 1 Score: 172.9 bits (437), Expect = 5.1e-40
Identity = 100/166 (60.24%), Postives = 120/166 (72.29%), Query Frame = 1


Query: 28  GRFTWSSEGEVISKSLIDRFLVSNKWEEAFEKRKAFSTSSLSAVMLKIVGLH-YLNFLAW 87
           G F + +EG ++ + L +RF V+ +   A  +     T   SA  L   G H ++N+   
Sbjct: 312 GDFCYVNEGLLLGQLLGNRFTVTLRGVVADSE----DTIKGSADSL---GRHGFINYFG- 371

Query: 88  DRRYVQRFGSGSVPTHLVGASLLRGEWKGAVNMILDPREGDILLLM-------RARDYYK 147
               +QRFGSGSVPTHL+GA+LLRGEWK AV+MILDPREGDILLL+        AR+YYK
Sbjct: 372 ----LQRFGSGSVPTHLIGAALLRGEWKYAVSMILDPREGDILLLVICFMWMRMAREYYK 431

Query: 148 ESDDIDGTLKQLPRYLVAERAILQCLKKCPGNYLQALKAIPRTLRM 186
           ESDDIDGTL+QLPRYL AERA+LQCLKKCPGNYLQALKAIPRTLRM
Sbjct: 432 ESDDIDGTLRQLPRYLTAERAVLQCLKKCPGNYLQALKAIPRTLRM 465

The following BLAST results are available for this feature:

BLAST of Cucsa.291730 vs. Swiss-Prot


Match Name	E-value	Identity	Description
YH2X_SCHPO	2.6e-12	40.63	Putative pseudouridine synthase C1A4.09 OS=Schizosaccharomyces pombe (strain 972...	[more]
PUS7_YEAST	1.8e-08	36.89	Multisubstrate pseudouridine synthase 7 OS=Saccharomyces cerevisiae (strain ATCC...	[more]
TRUD_HALMA	3.3e-07	38.78	Probable tRNA pseudouridine synthase D OS=Haloarcula marismortui (strain ATCC 43...	[more]
PUS7L_HUMAN	1.7e-06	33.67	Pseudouridylate synthase 7 homolog-like protein OS=Homo sapiens GN=PUS7L PE=1 SV...	[more]
TRUD_THEGJ	2.8e-06	34.83	Probable tRNA pseudouridine synthase D OS=Thermococcus gammatolerans (strain DSM...	[more]

BLAST of Cucsa.291730 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LY46_CUCSA	4.1e-44	94.95	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G690320 PE=4 SV=1	[more]
B9RER1_RICCO	3.6e-40	60.24	tRNA pseudouridine synthase d, putative OS=Ricinus communis GN=RCOM_1427850 PE=4...	[more]
V4V316_9ROSI	2.3e-39	87.37	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10003382mg PE=4 SV=1	[more]
F6HTS9_VITVI	1.9e-38	60.13	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_14s0030g01850 PE=4 SV=...	[more]
M5WRD4_PRUPE	1.9e-38	55.00	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa020985mg PE=3 SV=1	[more]

BLAST of Cucsa.291730 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G04820.1	1.0e-38	80.00	Pseudouridine synthase family protein	[more]

BLAST of Cucsa.291730 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700211699\|gb\|KGN66795.1\|	5.8e-44	94.95	hypothetical protein Csa_1G690320 [Cucumis sativus]	[more]
gi\|778664378\|ref\|XP_011660284.1\|	1.3e-43	61.64	PREDICTED: pseudouridylate synthase 7 homolog [Cucumis sativus]	[more]
gi\|659125504\|ref\|XP_008462716.1\|	2.9e-43	61.01	PREDICTED: pseudouridylate synthase 7 homolog isoform X1 [Cucumis melo]	[more]
gi\|659125508\|ref\|XP_008462718.1\|	2.9e-43	61.01	PREDICTED: pseudouridylate synthase 7 homolog isoform X2 [Cucumis melo]	[more]
gi\|223548191\|gb\|EEF49682.1\|	5.1e-40	60.24	tRNA pseudouridine synthase d, putative [Ricinus communis]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR001656	PsdUridine_synth_TruD
IPR011760	PsdUridine_synth_TruD_insert

Vocabulary: **Biological Process**
Term	Definition
GO:0001522	pseudouridine synthesis
GO:0009451	RNA modification

Vocabulary: **Molecular Function**
Term	Definition
GO:0003723	RNA binding
GO:0009982	pseudouridine synthase activity

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0001522	pseudouridine synthesis
biological_process	GO:0009451	RNA modification
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0009982	pseudouridine synthase activity
molecular_function	GO:0003723	RNA binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsa.291730.1	Cucsa.291730.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001656	Pseudouridine synthase, TruD	PANTHER	PTHR13326	TRNA PSEUDOURIDINE SYNTHASE D	coord: 92..185 score: 2.9
IPR001656	Pseudouridine synthase, TruD	PFAM	PF01142	TruD	coord: 91..184 score: 3.7
IPR011760	Pseudouridine synthase, TruD, insertion domain	PROFILE	PS50984	TRUD	coord: 84..186 score: 16

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cucsa.291730	Csa1G690320	Cucumber (Chinese Long) v2	cgycuB417
Cucsa.291730	CSPI01G33650	Wild cucumber (PI 183967)	cgycpiB440

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cucsa.291730	Cucumber (Chinese Long) v3	cgycucB450
Cucsa.291730	Cucumber (Chinese Long) v3	cgycucB451
Cucsa.291730	Watermelon (97103) v2	cgywmbB508
Cucsa.291730	Wax gourd	cgywgoB591
Cucsa.291730	Cucurbita maxima (Rimu)	cgycmaB0815
Cucsa.291730	Cucurbita maxima (Rimu)	cgycmaB0816
Cucsa.291730	Cucurbita moschata (Rifu)	cgycmoB0809
Cucsa.291730	Cucurbita moschata (Rifu)	cgycmoB0810
Cucsa.291730	Wild cucumber (PI 183967)	cgycpiB441
Cucsa.291730	Cucumber (Chinese Long) v2	cgycuB418
Cucsa.291730	Melon (DHL92) v3.5.1	cgymeB484
Cucsa.291730	Watermelon (Charleston Gray)	cgywcgB507
Cucsa.291730	Watermelon (97103) v1	cgywmB535
Cucsa.291730	Cucurbita pepo (Zucchini)	cgycpeB0771
Cucsa.291730	Cucurbita pepo (Zucchini)	cgycpeB0772
Cucsa.291730	Bottle gourd (USVL1VR-Ls)	cgylsiB473
Cucsa.291730	Bottle gourd (USVL1VR-Ls)	cgylsiB474
Cucsa.291730	Melon (DHL92) v3.6.1	cgymedB483
Cucsa.291730	Silver-seed gourd	carcgyB0025
Cucsa.291730	Silver-seed gourd	carcgyB1031