Cucsa.291720 (gene) Cucumber (Gy14) v1

Overview
NameCucsa.291720
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionPseudouridine synthase family protein
Locationscaffold02766 : 279768 .. 281600 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GGAAAAaGGAAAAGGAAAAAAaGCGCTGAAAAACCCTGAATGGGGTGGTACCGGCCAGGGGCATCGTCCTTCACTGTCCTAAAaCCCTACTTTCTCTATAACTCTTTAACCACTCCCAAACTCACCAATAGATCTTCTTCGATCGCAGTCATGACGACCAAGTCCGTGGGTGAATCAGATGTTGGCATACTCAGTTACATTTCTTCTCTCCCTGGCTTTCGCGGCATTCTCAAGCAAAGGTCTCAGCTTCTTACCCCCATGCATGCCCTTCATTTCTCAGGGATGCTATAACGCTCAATCCAATTTCGTACTATCTGCTTGATTGTATTGAAGATAAGGGTCAACTCTTAGAATGTGAAATGAAATGTTGAGAGTAAATTTAACTTATGAAACCGTTTTTCTGAATTGATAACAAAATCAGAATGAATGAGACCAAAATGGTAACTTCAACTGCCAATTGTTGTTATGAATTGGTCGACGTAACTGGCACCATGGGAATGTTCTTATGGTTTCATTGCCAGAGATTTTTTGTGGCCGTAATCTGTTCGGAGGAGTGTTTTTTTtCCTATCCCACTTTTGTTGTTTTATCATTTATTAATGAAGTTTCTGATTCCTCAGATAAAAAGGTTCGGTAGTAATGTGTCCATCGTATAGCATCTGTTGTTTGTTCTCGTTCTATTCTTGGAGTGGGAAATAGAGCAGTGAGCCTTTAGGTTGTTGTAGGAATGCTAATAGCAAATAGTATCATTCAACTATATTCTCATAAGGTTTGGATAAAGCCTAAAGGGACAATTTTTACTTTGTTTTTAAGATTTGAAGAAACTTTTACTCAACGATCCAGCTGTAAGACATTTTTTTAAAGCTTTTAGAACCCTATCATTGTAAATTCATCCTTTTTTGGTTTCGATACAGGTATTCTGATTTCATTGTGAATGAAGTTGACACTGATGGAAATGTTGTTCATTTGACGTCATTGGATGCTCCTCCAGAGGTGGTGAAGTTGAATTTCTGTGTTTGTATTGGATGCCTTAGATGTTGATTTCCTTCTTTTGCGTGAACATTTATGGTAATTTTAATTCTTTTGAGGCTTCAGATTGTTTCGGAAAGCGGACCAACGATAGTCCACAGTACAAGCCAGAGTTATGCTTCTGAAATTGAATTGTTTAGATCTCTAGCTGGTGATTCTGATGCTGAGATTTTGGAAATTTTTCTTAAGCAAATCAATTCTGGCGTGAATGATGACATTTTGCCTATTGTATTCTCCCCTGATACAGACAAAACTCATCGAACGGTTAGCTCTAAGACCTTGTTTGTTAATGTATTTGCACATTTATTTTTGCGTATCGTGCATTTGTTGATTCCGAGTAACAACCAGTGCAGGACATCATCTCTTTCTTCTTTTAGCTCATCTTTAGATTATTCCTTTAGTTTTTAACTAAAACTGTCCATTGTGGTACAGATATCTATATCTATTTTCAATGCTAAATATTTTAATTTTGCTCAAAGTGTAACTTCTCTTACCATTCAGTCAGATTGAACAACTAAACAAAGACATATCTTTGTTTTTCATCACCATATACTTTACTCTTTAAAATTGCGGTTTTCAGGCTGTCCACAATTTCTTTAAGAAATTTAAGTTCCTTGTGACCGATACTATTGATGGGCCAGATTCTTCATCAAAATGTGTCCGTGTGAGGGTGGATTCAGGGGCACAGAATAACCGAGGTCGTTTCTCTAAGAAACGGAAAGAAAGGGGCGATAAACCCTTTGATAGTAGAGGTTCGGACAACTGGCCTGAACATGTTGGCAAGTTTCTCAGGTAAGGTATTTTCT

mRNA sequence

ggaaaaaggaaaaggaaaaaaaGCGCTGAAAAACCCTGAATGGGGTGGTACCGGCCAGGGGCATCGTCCTTCACTGTCCTAAAACCCTACTTTCTCTATAACTCTTTAACCACTCCCAAACTCACCAATAGATCTTCTTCGATCGCAGTCATGACGACCAAGTCCGTGGGTGAATCAGATGTTGGCATACTCAGTTACATTTCTTCTCTCCCTGGCTTTCGCGGCATTCTCAAGCAAAGGTATTCTGATTTCATTGTGAATGAAGTTGACACTGATGGAAATGTTGTTCATTTGACGTCATTGGATGCTCCTCCAGAGATTGTTTCGGAAAGCGGACCAACGATAGTCCACAGTACAAGCCAGAGTTATGCTTCTGAAATTGAATTGTTTAGATCTCTAGCTGGTGATTCTGATGCTGAGATTTTGGAAATTTTTCTTAAGCAAATCAATTCTGGCGTGAATGATGACATTTTGCCTATTGTATTCTCCCCTGATACAGACAAAACTCATCGAACGGCTGTCCACAATTTCTTTAAGAAATTTAAGTTCCTTGTGACCGATACTATTGATGGGCCAGATTCTTCATCAAAATGTGTCCGTGTGAGGGTGGATTCAGGGGCACAGAATAACCGAGGTCGTTTCTCTAAGAAACGGAAAGAAAGGGGCGATAAACCCTTTGATAGTAGAGGTTCGGACAACTGGCCTGAACATGTTGGCAAGTTTCTCAGGTAAGGTATTTTCT

Coding sequence (CDS)

ATGGGGTGGTACCGGCCAGGGGCATCGTCCTTCACTGTCCTAAAaCCCTACTTTCTCTATAACTCTTTAACCACTCCCAAACTCACCAATAGATCTTCTTCGATCGCAGTCATGACGACCAAGTCCGTGGGTGAATCAGATGTTGGCATACTCAGTTACATTTCTTCTCTCCCTGGCTTTCGCGGCATTCTCAAGCAAAGGTATTCTGATTTCATTGTGAATGAAGTTGACACTGATGGAAATGTTGTTCATTTGACGTCATTGGATGCTCCTCCAGAGATTGTTTCGGAAAGCGGACCAACGATAGTCCACAGTACAAGCCAGAGTTATGCTTCTGAAATTGAATTGTTTAGATCTCTAGCTGGTGATTCTGATGCTGAGATTTTGGAAATTTTTCTTAAGCAAATCAATTCTGGCGTGAATGATGACATTTTGCCTATTGTATTCTCCCCTGATACAGACAAAACTCATCGAACGGCTGTCCACAATTTCTTTAAGAAATTTAAGTTCCTTGTGACCGATACTATTGATGGGCCAGATTCTTCATCAAAATGTGTCCGTGTGAGGGTGGATTCAGGGGCACAGAATAACCGAGGTCGTTTCTCTAAGAAACGGAAAGAAAGGGGCGATAAACCCTTTGATAGTAGAGGTTCGGACAACTGGCCTGAACATGTTGGCAAGTTTCTCAGGTAA

Protein sequence

MGWYRPGASSFTVLKPYFLYNSLTTPKLTNRSSSIAVMTTKSVGESDVGILSYISSLPGFRGILKQRYSDFIVNEVDTDGNVVHLTSLDAPPEIVSESGPTIVHSTSQSYASEIELFRSLAGDSDAEILEIFLKQINSGVNDDILPIVFSPDTDKTHRTAVHNFFKKFKFLVTDTIDGPDSSSKCVRVRVDSGAQNNRGRFSKKRKERGDKPFDSRGSDNWPEHVGKFLR*
Homology
[Close]
BLAST of Cucsa.291720 vs. Swiss-Prot
Match: PUS7_HUMAN (Pseudouridylate synthase 7 homolog OS=Homo sapiens GN=PUS7 PE=1 SV=2)

HSP 1 Score: 71.2 bits (173), Expect = 1.6e-11
Identity = 39/101 (38.61%), Postives = 58/101 (57.43%), Query Frame = 1 

Query: 32  SSSIAVMTTKSVGESDVGILSYISSLPGFRGILKQRYSDFIVNEVDTDGNVVHLTSLDAP 91
           S S A M    + E+DVGI  ++SS  GF GILK+RYSDF+V+E+  DG + HL  L  P
Sbjct: 98  SESFADMMKHGLTEADVGITKFVSSHQGFSGILKERYSDFVVHEIGKDGRISHLNDLSIP 157

Query: 92  --PEIVSESGPTIVHSTSQSYASEIELFRSLAGDSDAEILE 131
              E  SE   T++ +  +    E++LF++       E++E
Sbjct: 158 VDEEDPSEDIFTVLTAEEKQRLEELQLFKNKETSVAIEVIE 198

[Close]
BLAST of Cucsa.291720 vs. Swiss-Prot
Match: PUS7_BOVIN (Pseudouridylate synthase 7 homolog OS=Bos taurus GN=PUS7 PE=2 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 3.6e-11
Identity = 38/101 (37.62%), Postives = 58/101 (57.43%), Query Frame = 1 

Query: 32  SSSIAVMTTKSVGESDVGILSYISSLPGFRGILKQRYSDFIVNEVDTDGNVVHLTSLDAP 91
           + S A M    + E DVGI  ++SS  GF GILK+RYSDF+V+E+  DG + HL+ L  P
Sbjct: 96  AESFADMMKHGLTELDVGITKFVSSHQGFSGILKERYSDFVVHEIGRDGRISHLSDLSVP 155

Query: 92  --PEIVSESGPTIVHSTSQSYASEIELFRSLAGDSDAEILE 131
              E  SE   T++ +  +    E++LF++       E++E
Sbjct: 156 VDEEDPSEDVFTVLTAEEKQRLEELQLFKNKETSVAIEVIE 196

[Close]
BLAST of Cucsa.291720 vs. Swiss-Prot
Match: PUS7_YEAST (Multisubstrate pseudouridine synthase 7 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PUS7 PE=1 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 6.8e-10
Identity = 54/192 (28.12%), Postives = 98/192 (51.04%), Query Frame = 1 

Query: 43  VGESDVGILSYIS-SLPGFRGILKQRYSDFIVNEVDTDGNVVHLTSLDAPPEIVSESGPT 102
           + E+DVGI  ++S  LPGFRG +KQRY+DF+VNE+D +G V+HLT          +    
Sbjct: 38  IHEADVGITLFLSPELPGFRGQIKQRYTDFLVNEIDQEGKVIHLTDKGFKMPKKPQRSKE 97

Query: 103 IVHSTSQSYASEIELFRSLAGDSDAEILEIFLKQINSGVNDDILPIVFSPDT-------- 162
            V++  +S A+  + F ++  +   +++EIF         +D+L I     T        
Sbjct: 98  EVNAEKESEAARRQEF-NVDPELRNQLVEIF-------GEEDVLKIESVYRTANKMETAK 157

Query: 163 ---DKTHRTAVHNFFKK-FKFLVTDTIDGPDSSSKCVRVRVDSGAQNNRGRFSKKRKERG 222
              DK+ RT +H   ++ FK    + ++   + +   ++     A++NR   + K +E+ 
Sbjct: 158 NFEDKSVRTKIHQLLREAFK----NELESVTTDTNTFKI-----ARSNRNSRTNK-QEKI 211

[Close]
BLAST of Cucsa.291720 vs. Swiss-Prot
Match: YH2X_SCHPO (Putative pseudouridine synthase C1A4.09 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=SPBC1A4.09 PE=3 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 3.7e-08
Identity = 29/55 (52.73%), Postives = 43/55 (78.18%), Query Frame = 1 

Query: 45  ESDVGILSYIS-SLPGFRGILKQRYSDFIVNEVDTDGNVVHLTSLDAPPEIVSES 99
           ESDVGI ++++ +LP   GI+K R++DF V EVDTDGN+VHLT ++A   I+S++
Sbjct: 49  ESDVGIDAFLNPNLPSIDGIIKARFTDFAVFEVDTDGNIVHLTDMEAHDPILSKA 103

[Close]
BLAST of Cucsa.291720 vs. TrEMBL
Match: A0A0A0M0H8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G690310 PE=4 SV=1)

HSP 1 Score: 462.6 bits (1189), Expect = 2.8e-127
Identity = 230/230 (100.00%), Postives = 230/230 (100.00%), Query Frame = 1 

Query: 1   MGWYRPGASSFTVLKPYFLYNSLTTPKLTNRSSSIAVMTTKSVGESDVGILSYISSLPGF 60
           MGWYRPGASSFTVLKPYFLYNSLTTPKLTNRSSSIAVMTTKSVGESDVGILSYISSLPGF
Sbjct: 1   MGWYRPGASSFTVLKPYFLYNSLTTPKLTNRSSSIAVMTTKSVGESDVGILSYISSLPGF 60

Query: 61  RGILKQRYSDFIVNEVDTDGNVVHLTSLDAPPEIVSESGPTIVHSTSQSYASEIELFRSL 120
           RGILKQRYSDFIVNEVDTDGNVVHLTSLDAPPEIVSESGPTIVHSTSQSYASEIELFRSL
Sbjct: 61  RGILKQRYSDFIVNEVDTDGNVVHLTSLDAPPEIVSESGPTIVHSTSQSYASEIELFRSL 120

Query: 121 AGDSDAEILEIFLKQINSGVNDDILPIVFSPDTDKTHRTAVHNFFKKFKFLVTDTIDGPD 180
           AGDSDAEILEIFLKQINSGVNDDILPIVFSPDTDKTHRTAVHNFFKKFKFLVTDTIDGPD
Sbjct: 121 AGDSDAEILEIFLKQINSGVNDDILPIVFSPDTDKTHRTAVHNFFKKFKFLVTDTIDGPD 180

Query: 181 SSSKCVRVRVDSGAQNNRGRFSKKRKERGDKPFDSRGSDNWPEHVGKFLR 231
           SSSKCVRVRVDSGAQNNRGRFSKKRKERGDKPFDSRGSDNWPEHVGKFLR
Sbjct: 181 SSSKCVRVRVDSGAQNNRGRFSKKRKERGDKPFDSRGSDNWPEHVGKFLR 230

[Close]
BLAST of Cucsa.291720 vs. TrEMBL
Match: W9QVC1_9ROSA (Multisubstrate pseudouridine synthase 7 OS=Morus notabilis GN=L484_006163 PE=4 SV=1)

HSP 1 Score: 294.7 bits (753), Expect = 1.0e-76
Identity = 150/224 (66.96%), Postives = 179/224 (79.91%), Query Frame = 1 

Query: 8   ASSFTVLKPYFLYNSLTTPKLTNRSSSIAVMTTKSVGESDVGILSYISSLPGFRGILKQR 67
           +S  +VLKPY L N  TT   ++++ + A+   K++ ESDVGI  YIS+LPGFRGILKQR
Sbjct: 32  SSYISVLKPYSLCN-YTTIAASHKALTTAM---KTLDESDVGIFCYISNLPGFRGILKQR 91

Query: 68  YSDFIVNEVDTDGNVVHLTSLDAPPEIVSESGPTIVHSTSQSYASEIELFRSLAGDSDAE 127
           YSDFIVNEVDTDGNVVHLTSLDAPPE   E+    + + ++SY SEIE FR LAGDSDAE
Sbjct: 92  YSDFIVNEVDTDGNVVHLTSLDAPPETREETRTETIDAGTKSYTSEIESFRCLAGDSDAE 151

Query: 128 ILEIFLKQINSGVNDDILPIVFSPDTDKTHRTAVHNFFKK-FKFLVTDTIDGPDSSSKCV 187
            LE  + QINSG  +++ PIV SP +DK+HRT +HNFFKK FKFLVTDT+DGPD+SSKC+
Sbjct: 152 SLEALINQINSGGGEEVSPIVLSPSSDKSHRTEIHNFFKKNFKFLVTDTVDGPDASSKCI 211

Query: 188 RVRVDSGAQNNRGRFSKKRKERGDKPFDSRGSDNWPEHVGKFLR 231
           RVR++SG  NN GR SKKRKERGDKPFD RGSD+WPEHVGKFLR
Sbjct: 212 RVRLNSGRHNNHGRNSKKRKERGDKPFDRRGSDDWPEHVGKFLR 251

[Close]
BLAST of Cucsa.291720 vs. TrEMBL
Match: A0A061F1A0_THECC (Pseudouridine synthase family protein isoform 3 OS=Theobroma cacao GN=TCM_026386 PE=4 SV=1)

HSP 1 Score: 284.6 bits (727), Expect = 1.0e-73
Identity = 147/230 (63.91%), Postives = 178/230 (77.39%), Query Frame = 1 

Query: 12  TVLKPYFLYN----------SLTTPKLTNRSSSIAVMTTKSVGESDVGILSYISSLPGFR 71
           ++LKPY  ++          +LT   +   S+S+ +MTT  + ESDVGIL +IS LPGFR
Sbjct: 7   SILKPYHYFHCKSCPADSKSNLTKTPVIKSSNSLTLMTT--ITESDVGILCFISHLPGFR 66

Query: 72  GILKQRYSDFIVNEVDTDGNVVHLTSLDAPPEIVSESGPTIVHSTSQSYASEIELFRSLA 131
           GILKQRYSDFIVNEVDT+GNVVHLTSLDAPPE V E         ++ YASEI  FRSLA
Sbjct: 67  GILKQRYSDFIVNEVDTEGNVVHLTSLDAPPEAVQEGETKTPDQLNKCYASEIIKFRSLA 126

Query: 132 GDSDAEILEIFLKQINSGVNDDILPIVFSPDTDKTHRTAVHNFFKK-FKFLVTDTIDGPD 191
           GD+DA+ LE F+ QI SG  +DI PIV SPD+DK+HRTA+HNFFK+  +FLVTDT+DGPD
Sbjct: 127 GDTDADRLEAFINQITSGSEEDICPIVLSPDSDKSHRTAMHNFFKENLRFLVTDTVDGPD 186

Query: 192 SSSKCVRVRVDSGAQNNRGRFSKKRKERGDKPFDSRGSDNWPEHVGKFLR 231
           SSSKC+RVR++SG  NN+GR SKKRKERGDKP+DSRGS +WPEH+GKFLR
Sbjct: 187 SSSKCIRVRLNSG-NNNKGRNSKKRKERGDKPYDSRGSSHWPEHLGKFLR 233

[Close]
BLAST of Cucsa.291720 vs. TrEMBL
Match: A0A061F9D9_THECC (Pseudouridine synthase family protein isoform 2 OS=Theobroma cacao GN=TCM_026386 PE=4 SV=1)

HSP 1 Score: 284.6 bits (727), Expect = 1.0e-73
Identity = 147/230 (63.91%), Postives = 178/230 (77.39%), Query Frame = 1 

Query: 12  TVLKPYFLYN----------SLTTPKLTNRSSSIAVMTTKSVGESDVGILSYISSLPGFR 71
           ++LKPY  ++          +LT   +   S+S+ +MTT  + ESDVGIL +IS LPGFR
Sbjct: 7   SILKPYHYFHCKSCPADSKSNLTKTPVIKSSNSLTLMTT--ITESDVGILCFISHLPGFR 66

Query: 72  GILKQRYSDFIVNEVDTDGNVVHLTSLDAPPEIVSESGPTIVHSTSQSYASEIELFRSLA 131
           GILKQRYSDFIVNEVDT+GNVVHLTSLDAPPE V E         ++ YASEI  FRSLA
Sbjct: 67  GILKQRYSDFIVNEVDTEGNVVHLTSLDAPPEAVQEGETKTPDQLNKCYASEIIKFRSLA 126

Query: 132 GDSDAEILEIFLKQINSGVNDDILPIVFSPDTDKTHRTAVHNFFKK-FKFLVTDTIDGPD 191
           GD+DA+ LE F+ QI SG  +DI PIV SPD+DK+HRTA+HNFFK+  +FLVTDT+DGPD
Sbjct: 127 GDTDADRLEAFINQITSGSEEDICPIVLSPDSDKSHRTAMHNFFKENLRFLVTDTVDGPD 186

Query: 192 SSSKCVRVRVDSGAQNNRGRFSKKRKERGDKPFDSRGSDNWPEHVGKFLR 231
           SSSKC+RVR++SG  NN+GR SKKRKERGDKP+DSRGS +WPEH+GKFLR
Sbjct: 187 SSSKCIRVRLNSG-NNNKGRNSKKRKERGDKPYDSRGSSHWPEHLGKFLR 233

[Close]
BLAST of Cucsa.291720 vs. TrEMBL
Match: A0A061F2I3_THECC (Pseudouridine synthase family protein, putative isoform 1 OS=Theobroma cacao GN=TCM_026386 PE=4 SV=1)

HSP 1 Score: 276.9 bits (707), Expect = 2.2e-71
Identity = 147/243 (60.49%), Postives = 179/243 (73.66%), Query Frame = 1 

Query: 12  TVLKPYFLYN----------SLTTPKLTNRSSSIAVMTTKSVGESDVGILSYISSLPGFR 71
           ++LKPY  ++          +LT   +   S+S+ +MTT  + ESDVGIL +IS LPGFR
Sbjct: 7   SILKPYHYFHCKSCPADSKSNLTKTPVIKSSNSLTLMTT--ITESDVGILCFISHLPGFR 66

Query: 72  GILKQRYSDFIVNEVDTDGNVVHLTSLDAPPEI-------------VSESGPTIVHSTSQ 131
           GILKQRYSDFIVNEVDT+GNVVHLTSLDAPPE+             V E         ++
Sbjct: 67  GILKQRYSDFIVNEVDTEGNVVHLTSLDAPPEVMIDVFSLNKFTQAVQEGETKTPDQLNK 126

Query: 132 SYASEIELFRSLAGDSDAEILEIFLKQINSGVNDDILPIVFSPDTDKTHRTAVHNFFKK- 191
            YASEI  FRSLAGD+DA+ LE F+ QI SG  +DI PIV SPD+DK+HRTA+HNFFK+ 
Sbjct: 127 CYASEIIKFRSLAGDTDADRLEAFINQITSGSEEDICPIVLSPDSDKSHRTAMHNFFKEN 186

Query: 192 FKFLVTDTIDGPDSSSKCVRVRVDSGAQNNRGRFSKKRKERGDKPFDSRGSDNWPEHVGK 231
            +FLVTDT+DGPDSSSKC+RVR++SG  NN+GR SKKRKERGDKP+DSRGS +WPEH+GK
Sbjct: 187 LRFLVTDTVDGPDSSSKCIRVRLNSG-NNNKGRNSKKRKERGDKPYDSRGSSHWPEHLGK 246

[Close]
BLAST of Cucsa.291720 vs. TAIR10
Match: AT3G04820.1 (AT3G04820.1 Pseudouridine synthase family protein)

HSP 1 Score: 262.3 bits (669), Expect = 2.8e-70
Identity = 135/223 (60.54%), Postives = 171/223 (76.68%), Query Frame = 1 

Query: 12  TVLKPYFLYNSLT-TPKLTNRSSSIAVMTTKSVGESDVGILSYISSLPGFRGILKQRYSD 71
           ++LKPY   +SL+ T K+   +++   M  K+V E+DVGI  +IS LPGFRGILKQRY+D
Sbjct: 13  SLLKPY---SSLSWTTKIATIATNFEGM--KTVDEADVGISCFISQLPGFRGILKQRYAD 72

Query: 72  FIVNEVDTDGNVVHLTSLDAPPEIV--SESGPTIVHSTSQSYASEIELFRSLAGDSDAEI 131
           FIVNEVD DGNVVHLTSLD PPE+    E       +  Q+Y S +E F+S++  +DAE 
Sbjct: 73  FIVNEVDMDGNVVHLTSLDLPPEMAVKEEDKAKSCDNVPQNYTSAVESFKSISSVTDAER 132

Query: 132 LEIFLKQINSGVNDDILPIVFSPDTDKTHRTAVHNFFKK-FKFLVTDTIDGPDSSSKCVR 191
           LEI + Q+ SG + ++LP+V  P +DK+ RTA+HNF K+ FKFLVTDT+DGPDS+SKC+R
Sbjct: 133 LEILINQVTSGTDGEVLPVVLDPSSDKSKRTAIHNFVKENFKFLVTDTVDGPDSTSKCIR 192

Query: 192 VRVDSGAQNNRGRFSKKRKERGDKPFDSRGSDNWPEHVGKFLR 231
           VRV+SGA NNRG  SKKRKERGDKPFDSRGS++WPEHVGKFLR
Sbjct: 193 VRVNSGANNNRGNRSKKRKERGDKPFDSRGSEHWPEHVGKFLR 230

[Close]
BLAST of Cucsa.291720 vs. NCBI nr
Match: gi|778664378|ref|XP_011660284.1| (PREDICTED: pseudouridylate synthase 7 homolog [Cucumis sativus])

HSP 1 Score: 462.6 bits (1189), Expect = 4.0e-127
Identity = 230/230 (100.00%), Postives = 230/230 (100.00%), Query Frame = 1 

Query: 1   MGWYRPGASSFTVLKPYFLYNSLTTPKLTNRSSSIAVMTTKSVGESDVGILSYISSLPGF 60
           MGWYRPGASSFTVLKPYFLYNSLTTPKLTNRSSSIAVMTTKSVGESDVGILSYISSLPGF
Sbjct: 1   MGWYRPGASSFTVLKPYFLYNSLTTPKLTNRSSSIAVMTTKSVGESDVGILSYISSLPGF 60

Query: 61  RGILKQRYSDFIVNEVDTDGNVVHLTSLDAPPEIVSESGPTIVHSTSQSYASEIELFRSL 120
           RGILKQRYSDFIVNEVDTDGNVVHLTSLDAPPEIVSESGPTIVHSTSQSYASEIELFRSL
Sbjct: 61  RGILKQRYSDFIVNEVDTDGNVVHLTSLDAPPEIVSESGPTIVHSTSQSYASEIELFRSL 120

Query: 121 AGDSDAEILEIFLKQINSGVNDDILPIVFSPDTDKTHRTAVHNFFKKFKFLVTDTIDGPD 180
           AGDSDAEILEIFLKQINSGVNDDILPIVFSPDTDKTHRTAVHNFFKKFKFLVTDTIDGPD
Sbjct: 121 AGDSDAEILEIFLKQINSGVNDDILPIVFSPDTDKTHRTAVHNFFKKFKFLVTDTIDGPD 180

Query: 181 SSSKCVRVRVDSGAQNNRGRFSKKRKERGDKPFDSRGSDNWPEHVGKFLR 231
           SSSKCVRVRVDSGAQNNRGRFSKKRKERGDKPFDSRGSDNWPEHVGKFLR
Sbjct: 181 SSSKCVRVRVDSGAQNNRGRFSKKRKERGDKPFDSRGSDNWPEHVGKFLR 230

[Close]
BLAST of Cucsa.291720 vs. NCBI nr
Match: gi|700211698|gb|KGN66794.1| (hypothetical protein Csa_1G690310 [Cucumis sativus])

HSP 1 Score: 462.6 bits (1189), Expect = 4.0e-127
Identity = 230/230 (100.00%), Postives = 230/230 (100.00%), Query Frame = 1 

Query: 1   MGWYRPGASSFTVLKPYFLYNSLTTPKLTNRSSSIAVMTTKSVGESDVGILSYISSLPGF 60
           MGWYRPGASSFTVLKPYFLYNSLTTPKLTNRSSSIAVMTTKSVGESDVGILSYISSLPGF
Sbjct: 1   MGWYRPGASSFTVLKPYFLYNSLTTPKLTNRSSSIAVMTTKSVGESDVGILSYISSLPGF 60

Query: 61  RGILKQRYSDFIVNEVDTDGNVVHLTSLDAPPEIVSESGPTIVHSTSQSYASEIELFRSL 120
           RGILKQRYSDFIVNEVDTDGNVVHLTSLDAPPEIVSESGPTIVHSTSQSYASEIELFRSL
Sbjct: 61  RGILKQRYSDFIVNEVDTDGNVVHLTSLDAPPEIVSESGPTIVHSTSQSYASEIELFRSL 120

Query: 121 AGDSDAEILEIFLKQINSGVNDDILPIVFSPDTDKTHRTAVHNFFKKFKFLVTDTIDGPD 180
           AGDSDAEILEIFLKQINSGVNDDILPIVFSPDTDKTHRTAVHNFFKKFKFLVTDTIDGPD
Sbjct: 121 AGDSDAEILEIFLKQINSGVNDDILPIVFSPDTDKTHRTAVHNFFKKFKFLVTDTIDGPD 180

Query: 181 SSSKCVRVRVDSGAQNNRGRFSKKRKERGDKPFDSRGSDNWPEHVGKFLR 231
           SSSKCVRVRVDSGAQNNRGRFSKKRKERGDKPFDSRGSDNWPEHVGKFLR
Sbjct: 181 SSSKCVRVRVDSGAQNNRGRFSKKRKERGDKPFDSRGSDNWPEHVGKFLR 230

[Close]
BLAST of Cucsa.291720 vs. NCBI nr
Match: gi|659125504|ref|XP_008462716.1| (PREDICTED: pseudouridylate synthase 7 homolog isoform X1 [Cucumis melo])

HSP 1 Score: 445.7 bits (1145), Expect = 5.1e-122
Identity = 221/230 (96.09%), Postives = 222/230 (96.52%), Query Frame = 1 

Query: 1   MGWYRPGASSFTVLKPYFLYNSLTTPKLTNRSSSIAVMTTKSVGESDVGILSYISSLPGF 60
           MGWYRPGASSFTVLKPYFL NSLT PKLTNRSSSIAVMTTKS+GESDVGILSYISSLPGF
Sbjct: 1   MGWYRPGASSFTVLKPYFLCNSLTAPKLTNRSSSIAVMTTKSIGESDVGILSYISSLPGF 60

Query: 61  RGILKQRYSDFIVNEVDTDGNVVHLTSLDAPPEIVSESGPTIVHSTSQSYASEIELFRSL 120
           RGILKQRYSDFIVNEVDTDGNVVHLTSLDAPPEIVSESGPTIVHSTSQSY SEIELFRSL
Sbjct: 61  RGILKQRYSDFIVNEVDTDGNVVHLTSLDAPPEIVSESGPTIVHSTSQSYVSEIELFRSL 120

Query: 121 AGDSDAEILEIFLKQINSGVNDDILPIVFSPDTDKTHRTAVHNFFKKFKFLVTDTIDGPD 180
           AGDSDAEILE FLKQINSG NDDI PIVFSPDT KTHRTAVHNFFKKFKFLVTDTIDGPD
Sbjct: 121 AGDSDAEILETFLKQINSGANDDISPIVFSPDTSKTHRTAVHNFFKKFKFLVTDTIDGPD 180

Query: 181 SSSKCVRVRVDSGAQNNRGRFSKKRKERGDKPFDSRGSDNWPEHVGKFLR 231
           SSSKCVRVRVDSG QNNRGRFSKKRKERGDKPFDSRGSDNWPEHVGKFLR
Sbjct: 181 SSSKCVRVRVDSGVQNNRGRFSKKRKERGDKPFDSRGSDNWPEHVGKFLR 230

[Close]
BLAST of Cucsa.291720 vs. NCBI nr
Match: gi|659125508|ref|XP_008462718.1| (PREDICTED: pseudouridylate synthase 7 homolog isoform X2 [Cucumis melo])

HSP 1 Score: 377.9 bits (969), Expect = 1.3e-101
Identity = 187/194 (96.39%), Postives = 188/194 (96.91%), Query Frame = 1 

Query: 37  VMTTKSVGESDVGILSYISSLPGFRGILKQRYSDFIVNEVDTDGNVVHLTSLDAPPEIVS 96
           VMTTKS+GESDVGILSYISSLPGFRGILKQRYSDFIVNEVDTDGNVVHLTSLDAPPEIVS
Sbjct: 7   VMTTKSIGESDVGILSYISSLPGFRGILKQRYSDFIVNEVDTDGNVVHLTSLDAPPEIVS 66

Query: 97  ESGPTIVHSTSQSYASEIELFRSLAGDSDAEILEIFLKQINSGVNDDILPIVFSPDTDKT 156
           ESGPTIVHSTSQSY SEIELFRSLAGDSDAEILE FLKQINSG NDDI PIVFSPDT KT
Sbjct: 67  ESGPTIVHSTSQSYVSEIELFRSLAGDSDAEILETFLKQINSGANDDISPIVFSPDTSKT 126

Query: 157 HRTAVHNFFKKFKFLVTDTIDGPDSSSKCVRVRVDSGAQNNRGRFSKKRKERGDKPFDSR 216
           HRTAVHNFFKKFKFLVTDTIDGPDSSSKCVRVRVDSG QNNRGRFSKKRKERGDKPFDSR
Sbjct: 127 HRTAVHNFFKKFKFLVTDTIDGPDSSSKCVRVRVDSGVQNNRGRFSKKRKERGDKPFDSR 186

Query: 217 GSDNWPEHVGKFLR 231
           GSDNWPEHVGKFLR
Sbjct: 187 GSDNWPEHVGKFLR 200

[Close]
BLAST of Cucsa.291720 vs. NCBI nr
Match: gi|694375900|ref|XP_009364526.1| (PREDICTED: multisubstrate pseudouridine synthase 7 [Pyrus x bretschneideri])

HSP 1 Score: 302.4 bits (773), Expect = 6.9e-79
Identity = 153/229 (66.81%), Postives = 180/229 (78.60%), Query Frame = 1 

Query: 3   WYRPGASSFTVLKPYFLYNSLTTPKLTNRSSSIAVMTTKSVGESDVGILSYISSLPGFRG 62
           W +P  S F +LKPY    S T P     ++  A+M TK+  ESDVGI+ YIS LPGFRG
Sbjct: 2   WLKPAPSCFPMLKPYC---SATPPPPPLTTALKALMATKTTEESDVGIVCYISQLPGFRG 61

Query: 63  ILKQRYSDFIVNEVDTDGNVVHLTSLDAPPEIVSESGPTIVHSTSQSYASEIELFRSLAG 122
           ILKQRYSDF+VNEVD +GNVVHLT+LDAP E V ES  T    TS+ Y +EIE FR+LA 
Sbjct: 62  ILKQRYSDFMVNEVDKEGNVVHLTNLDAPVEAVQESATTTGDGTSKDYTTEIESFRALAD 121

Query: 123 DSDAEILEIFLKQINSGVNDDILPIVFSPDTDKTHRTAVHNFFKK-FKFLVTDTIDGPDS 182
            +DAE LE F+KQINSG  D ++PIV SPD DK+HRTAVHNFFK+ FKFLVTDT+DGPDS
Sbjct: 122 RNDAERLETFIKQINSGGEDGVMPIVLSPDYDKSHRTAVHNFFKQHFKFLVTDTVDGPDS 181

Query: 183 SSKCVRVRVDSGAQNNRGRFSKKRKERGDKPFDSRGSDNWPEHVGKFLR 231
           SSKC+RVR++SG QN+RGR S+KRKERGDKPFDSRGS++W EHVGKFLR
Sbjct: 182 SSKCIRVRINSGGQNSRGRNSRKRKERGDKPFDSRGSEDWSEHVGKFLR 227

The following BLAST results are available for this feature:
BLAST of Cucsa.291720 vs. Swiss-Prot
Match NameE-valueIdentityDescription
PUS7_HUMAN1.6e-1138.61Pseudouridylate synthase 7 homolog OS=Homo sapiens GN=PUS7 PE=1 SV=2[more]
PUS7_BOVIN3.6e-1137.62Pseudouridylate synthase 7 homolog OS=Bos taurus GN=PUS7 PE=2 SV=1[more]
PUS7_YEAST6.8e-1028.13Multisubstrate pseudouridine synthase 7 OS=Saccharomyces cerevisiae (strain ATCC... [more]
YH2X_SCHPO3.7e-0852.73Putative pseudouridine synthase C1A4.09 OS=Schizosaccharomyces pombe (strain 972... [more]
BLAST of Cucsa.291720 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0M0H8_CUCSA2.8e-127100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_1G690310 PE=4 SV=1[more]
W9QVC1_9ROSA1.0e-7666.96Multisubstrate pseudouridine synthase 7 OS=Morus notabilis GN=L484_006163 PE=4 S... [more]
A0A061F1A0_THECC1.0e-7363.91Pseudouridine synthase family protein isoform 3 OS=Theobroma cacao GN=TCM_026386... [more]
A0A061F9D9_THECC1.0e-7363.91Pseudouridine synthase family protein isoform 2 OS=Theobroma cacao GN=TCM_026386... [more]
A0A061F2I3_THECC2.2e-7160.49Pseudouridine synthase family protein, putative isoform 1 OS=Theobroma cacao GN=... [more]
BLAST of Cucsa.291720 vs. TAIR10
Match NameE-valueIdentityDescription
AT3G04820.12.8e-7060.54 Pseudouridine synthase family protein[more]
BLAST of Cucsa.291720 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|778664378|ref|XP_011660284.1|4.0e-127100.00PREDICTED: pseudouridylate synthase 7 homolog [Cucumis sativus][more]
gi|700211698|gb|KGN66794.1|4.0e-127100.00hypothetical protein Csa_1G690310 [Cucumis sativus][more]
gi|659125504|ref|XP_008462716.1|5.1e-12296.09PREDICTED: pseudouridylate synthase 7 homolog isoform X1 [Cucumis melo][more]
gi|659125508|ref|XP_008462718.1|1.3e-10196.39PREDICTED: pseudouridylate synthase 7 homolog isoform X2 [Cucumis melo][more]
gi|694375900|ref|XP_009364526.1|6.9e-7966.81PREDICTED: multisubstrate pseudouridine synthase 7 [Pyrus x bretschneideri][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001656PsdUridine_synth_TruD
IPR020103PsdUridine_synth_cat_dom_sf
IPR001656PsdUridine_synth_TruD
Vocabulary: Biological Process
TermDefinition
GO:0001522pseudouridine synthesis
GO:0009451RNA modification
GO:0001522pseudouridine synthesis
GO:0009451RNA modification
Vocabulary: Molecular Function
TermDefinition
GO:0003723RNA binding
GO:0009982pseudouridine synthase activity
GO:0003723RNA binding
GO:0009982pseudouridine synthase activity
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0001522 pseudouridine synthesis
biological_process GO:0009451 RNA modification
cellular_component GO:0005575 cellular_component
molecular_function GO:0009982 pseudouridine synthase activity
molecular_function GO:0003723 RNA binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.291720.1Cucsa.291720.1mRNA
Cucsa.291720.2Cucsa.291720.2mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001656Pseudouridine synthase, TruDPANTHERPTHR13326TRNA PSEUDOURIDINE SYNTHASE Dcoord: 31..147
score: 2.0
NoneNo IPR availablePANTHERPTHR13326:SF8SUBFAMILY NOT NAMEDcoord: 31..147
score: 2.0