Cucsa.281890 (gene) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.281890
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	Phospholipase A2 family protein
Location	scaffold02641 : 71072 .. 74217 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TGTCAAACCAAAATTCATTCAGTCTTTCCAACAACCAAAAGAACAAGAAAATGCCTCAATTTCTTCTTCTTCTTCTGTTGCCTTCCATTGTCTTTCTTCCTTCTCATGCTCTCAACATTGGTGTCCAAGCCCTTGATGCCTCCGTCACTATGGTTCTTCCTCATTTCATTCCCATCTTCTTTTTtCTTTAATTTGATCTTCTTCCTTCTTAATTGGTTTAATTTGTTCATATTGTAGAGTAAGGACTGCAGTAGAAAATGCGAGTCTGAGTTTTGTTCAGGTTAGTTTAAGAAGCTGTTGTTTGGAACAACTCTTTTTAATAACTCACTTGAAAAGTCAATTCAAACCAGTTCTGATTTATGTTTGATTTTGGTTCGAGCTTGTATTTGTATTTAATTGTTTGTGTGTGTTTTGTAGTGCCTCCACTGTTGAGATATGGGAAATATTGTGGGCTGTTGTATAGTGGGTGCCCCGGGGAACACCCCTGTGATGGCTTGGATGCTTGTTGTATGAAGCATGATGCTTGTGTTGTTGCCAAGAACGGTTAGTACTACACATACTTCTCTATTCCCATCCAATTCTTTTtCTTTCCAACAATGCGTTAGGTCTAGAATCCTCAAGGCTCAAATCTTTGGTCTGAGAGATTGACAGTACATATTTGATAATCAATATTGATGTCAGTTTTAATTTGTTTTTCAGTACAACAAATGGAGTAGAAAGAGTGTTACTTTTTCTAAATACACGGAGTCAATAGATAAATAAGCCTTAGAGAAATTAAGATAATGGCACAATTAATATGGAATTGACAAAATTTTGGAGAAAACCAATACTTGCTATGCTAGAGCTAGTTTTTGAATGGTTAATATTTATGATTTGGTCCTATTGAGTGAAGTTTCCTTTTATTAAAATTAATGAATTTGTGAGGATTTTATAGTTTTTTTtATTTTGTTAAAATTGAATTTAGAATCCAATTTGACGAAAATTTTAACTTTCAAATTTGTTACGCTATACGAGTTTTTACTATCAAAATTGTTTTATCGTTCTTATTACATTGTAGCACAATTTGATGTGTTCATTCGTTTATTGCTTTGGTTTAGTTCATATCTCATTAAAGTTGTTGGTATGATTTTAATGTGACGTGTTTGTTGAATAAACTTTGGTGTAATTAGTGTGATATGATAGGAAGTTGGACAAATGACGAATACGTCACATTCCAAAAGCGTTTTATAAATTATTTAGAAGTAGAGTTTGTATGGAATTATGTATTTGTTGTCATTTTAAAATAAATTACTAATTTAATTTTTAGTTTGTCAAAATAAACATATATGATATGTGTATTATCAATCTAAAAATTAAAACATATGATTTTGAGTACTCAATATTTTTCTTTtAAAATAATATTAGTAAAAATAAGACAATAATTTTAAAATAAACTAAAAAaTATTTATCAATTCAAGATTTTAAATTTGGTCACCAATTTTCTAAATGAGTGTTGTGGGGTGCTAATACCTTCTCCACACAAAAATGACTCTCGAACTCAAACTCTATTTTTGGCCGACTATTTTTTTtAAAAATTGTTTACTTTATTTCAGTGTCCAATCACACCCTAAAAAAGATTGGTGGCGATTCCTATATGTTTTAAAAAaTAACTCTTTTAAGGACGTCAACCACCTTGTGTTGTTTCGGCAGATGGATGGTGACACATACTTCTGATTGAATTTTGTCTGATCAATAATTTTGAAGAGTTTAGGGTATATGTGATGAGATGGCTAAATTTGTCTCTTTGCTTTGAGTGAACCAAAAAGGGTCACATGTAAAGGTGGCTATGAAGATCCTTTTTTAAAGCTTAATTTTATGGTTTATTTATTAAAAGAAAAAaCATGAAATTAAATTCAAGACTTTTTAATTAATTTTCATTTTCAATATATATATTGTTTAATTAATGTGCAATAGACTTAGGGTTGGAGACCAAATTGAAATAGAGAATTGTTCTCATTGGAATGTGAGGAATTTCTACAAACATCAAAATTTGGATTTTGAACCTTTTCCAATAAAGAAAAATATTAATAATAAAATGGGCCCAAAAAGAAAAAAAaCTTTTGAAATATTTAACTTGGTTATTCAACTTTCCCTTTTATTTGATTTTAGTTTAAACTCTTCCAACTTGTTAACTTAAATTAATCCAAAGTTTTTTTTtAAAAAAaTAATACTCCAATTTAAAAaTAAAAAaGAAAAAAATAATTAAGTGCAATCGAAGTTGCTCGGAATCCTATACTTTCGAGTTTCATCTTCTTTGCATGCTAAAAATAAAACATTTGCTACGTCTAAGGTGAGTCAACCCTACTTACGTATACGACGCTTTCCAATTATTATTTCTTTCAGGGTTCAACACAACACATGTTGTGTGAGAGATTTGAATCATTGATCTAATAGTAAAAACCCGACGTTCTTAATACATGAAGAAGTAGAAAAAAaTTGTGAGATTAGAAGTGAACCACAAAATTACTAGAAAACATCAAGAATTCACTTAAAATTTCAAGACGAGAGATAAAAAACTATGGTTCACTAGAATACGAAATCTCCTATGTAAAAACGTTACAATTCTCTTTGCTAATCCCAATTACAAGATCAACACCATTCCAAAGCGTTAAAGTCACTCACACAACTTAGTCCTCACTCCCAAAGAGAATAATCGACACAATTTGAAACCAAAaCAAAAaCTATAAACTCTTTTTGAAGTGTTTGGAGTACTCCACTGAAGTGGTGTATATGTGTTTTTGTTACATAATTTCAGATGACTATCTAAGCCAAGAATGCAGTCAAAGCTTCTTGAATTGCATGGAGAATTTCAAGAGATCAAGATCAAGTCCTTTCAAAGGAAACAAATGCCAAGTTGATGAAGTGATTGAAGTCATCTCCTTAGTCATGGAAGCTGCTCTTATTGCAGGCAGAGTTTTTCACAAACCCTAGCTAGCTAGCCACCCCTAACCTACCTTTTTATTTACAAATACCCAATTTCAATCATCAATCTCTTCCTTTTCCCCCCTTCATTATTATGTATTGTTGTTTGGCATAAAACACATACACCAATTTCTCTCATACATATGAAATCACTATTCTATAACATGTTTTGAAGTGTTCGTTTT

mRNA sequence

TGTCAAACCAAAATTCATTCAGTCTTTCCAACAACCAAAAGAACAAGAAAATGCCTCAATTTCTTCTTCTTCTTCTGTTGCCTTCCATTGTCTTTCTTCCTTCTCATGCTCTCAACATTGGTGTCCAAGCCCTTGATGCCTCCGTCACTATGAGTAAGGACTGCAGTAGAAAATGCGAGTCTGAGTTTTGTTCAGTGCCTCCACTGTTGAGATATGGGAAATATTGTGGGCTGTTGTATAGTGGGTGCCCCGGGGAACACCCCTGTGATGGCTTGGATGCTTGTTGTATGAAGCATGATGCTTGTGTTGTTGCCAAGAACGATGACTATCTAAGCCAAGAATGCAGTCAAAGCTTCTTGAATTGCATGGAGAATTTCAAGAGATCAAGATCAAGTCCTTTCAAAGGAAACAAATGCCAAGTTGATGAAGTGATTGAAGTCATCTCCTTAGTCATGGAAGCTGCTCTTATTGCAGGCAGAGTTTTTCACAAACCCTAGCTAGCTAGCCACCCCTAACCTACCTTTTTATTTACAAATACCCAATTTCAATCATCAATCTCTTCCTTTTCCCCCCTTCATTATTATGTATTGTTGTTTGGCATAAAACACATACACCAATTTCTCTCATACATATGAAATCACTATTCTATAACATGTTTTGAAGTGTTCGTTTT

Coding sequence (CDS)

ATGCCTCAATTTCTTCTTCTTCTTCTGTTGCCTTCCATTGTCTTTCTTCCTTCTCATGCTCTCAACATTGGTGTCCAAGCCCTTGATGCCTCCGTCACTATGAGTAAGGACTGCAGTAGAAAATGCGAGTCTGAGTTTTGTTCAGTGCCTCCACTGTTGAGATATGGGAAATATTGTGGGCTGTTGTATAGTGGGTGCCCCGGGGAACACCCCTGTGATGGCTTGGATGCTTGTTGTATGAAGCATGATGCTTGTGTTGTTGCCAAGAACGATGACTATCTAAGCCAAGAATGCAGTCAAAGCTTCTTGAATTGCATGGAGAATTTCAAGAGATCAAGATCAAGTCCTTTCAAAGGAAACAAATGCCAAGTTGATGAAGTGATTGAAGTCATCTCCTTAGTCATGGAAGCTGCTCTTATTGCAGGCAGAGTTTTTCACAAACCCTAG

Protein sequence

MPQFLLLLLLPSIVFLPSHALNIGVQALDASVTMSKDCSRKCESEFCSVPPLLRYGKYCGLLYSGCPGEHPCDGLDACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRSRSSPFKGNKCQVDEVIEVISLVMEAALIAGRVFHKP*

Homology

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BLAST of Cucsa.281890 vs. Swiss-Prot
Match: PLA2A_ARATH (Phospholipase A2-alpha OS=Arabidopsis thaliana GN=PLA2-ALPHA PE=1 SV=1)

HSP 1 Score: 220.7 bits (561), Expect = 1.1e-56
Identity = 96/129 (74.42%), Postives = 112/129 (86.82%), Query Frame = 1


Query: 20  ALNIGVQALDASVTMSKDCSRKCESEFCSVPPLLRYGKYCGLLYSGCPGEHPCDGLDACC 79
           ALN+GVQ +  S++++K+CSRKCESEFCSVPP LRYGKYCGLLYSGCPGE PCDGLD+CC
Sbjct: 20  ALNVGVQLIHPSISLTKECSRKCESEFCSVPPFLRYGKYCGLLYSGCPGERPCDGLDSCC 79

Query: 80  MKHDACVVAKNDDYLSQECSQSFLNCMENFKRSRSSPFKGNKCQVDEVIEVISLVMEAAL 139
           MKHDACV +KN+DYLSQECSQ F+NCM NF + +   FKGNKC  DEVI+VIS+VMEAAL
Sbjct: 80  MKHDACVQSKNNDYLSQECSQKFINCMNNFSQKKQPTFKGNKCDADEVIDVISIVMEAAL 139

Query: 140 IAGRVFHKP 149
           IAG+V  KP
Sbjct: 140 IAGKVLKKP 148

[Close]

BLAST of Cucsa.281890 vs. Swiss-Prot
Match: PLA23_ORYSJ (Phospholipase A2 homolog 3 OS=Oryza sativa subsp. japonica GN=PLA2-III PE=1 SV=1)

HSP 1 Score: 181.0 bits (458), Expect = 9.4e-45
Identity = 86/141 (60.99%), Postives = 106/141 (75.18%), Query Frame = 1


Query: 8   LLLPSIVFLPSHALNIGVQALDASVTMSKDCSRKCESEFCSVPPLLRYGKYCGLLYSGCP 67
           LLL ++V  P+ ALNIG+Q+     + +  CSR CES+ C+ PPLLRYGKYCG+LYSGCP
Sbjct: 25  LLLFAVVAPPAAALNIGLQSAGDGASKAGLCSRTCESDHCTTPPLLRYGKYCGILYSGCP 84

Query: 68  GEHPCDGLDACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRSRSSPFKGNKCQVDEV 127
           GE PCD LDACCM HD CV AKN DYLS  C++  L C+    R  SS F+GNKC +DEV
Sbjct: 85  GEQPCDELDACCMHHDNCVQAKN-DYLSTACNEELLECLARL-REGSSTFQGNKCMIDEV 144

Query: 128 IEVISLVMEAALIAGRVFHKP 149
           I+VISLV+EAA++AGR+ HKP
Sbjct: 145 IDVISLVIEAAVVAGRLLHKP 163

[Close]

BLAST of Cucsa.281890 vs. Swiss-Prot
Match: PLA22_ORYSJ (Probable phospholipase A2 homolog 2 OS=Oryza sativa subsp. japonica GN=PLA2-II PE=1 SV=1)

HSP 1 Score: 154.8 bits (390), Expect = 7.2e-37
Identity = 74/146 (50.68%), Postives = 98/146 (67.12%), Query Frame = 1


Query: 2   PQFLLLLLLPSIVFLPSHALNIGVQALDASVTMSKDCSRKCESEFCSVPPLLRYGKYCGL 61
           P+  +LLLL  ++   S  LNIG   L ++    + CSR CES+FC++ PLLRYGKYCG+
Sbjct: 9   PRCSVLLLL--LLVTASRGLNIG-DLLGSTPAKDQGCSRTCESQFCTIAPLLRYGKYCGI 68

Query: 62  LYSGCPGEHPCDGLDACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRSRSSPFKGNK 121
           LYSGCPGE PCD LDACCM HD CV   NDDYL+  C+++ L+C++   R   + F GNK
Sbjct: 69  LYSGCPGERPCDALDACCMVHDHCVDTHNDDYLNTMCNENLLSCID---RVSGATFPGNK 128

Query: 122 CQVDEVIEVISLVMEAALIAGRVFHK 148
           C V +   VI  V+E A+ AG++ HK
Sbjct: 129 CNVGQTASVIRGVIETAVFAGKILHK 148

[Close]

BLAST of Cucsa.281890 vs. Swiss-Prot
Match: PLA2D_ARATH (Phospholipase A2-delta OS=Arabidopsis thaliana GN=PLA2-DELTA PE=2 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 3.1e-16
Identity = 43/93 (46.24%), Postives = 56/93 (60.22%), Query Frame = 1


Query: 20  ALNIGVQALDASVTMSKDCSRKCESEFCSVPPLLRYGKYCGLLYSGCPGEHPCDGLDACC 79
           AL + V  L A V   + CS+ C ++ C+V   +RYGKYCG+ Y GCPGE PCD LD CC
Sbjct: 11  ALGLTVFLLLAVVHSQEKCSKTCIAQKCNVLG-IRYGKYCGIGYFGCPGEPPCDDLDDCC 70

Query: 80  MKHDACVVAKNDDYLSQECSQSFLNCMENFKRS 113
           M HD CV  K   Y+  +C + F  C+   K+S
Sbjct: 71  MTHDNCVDLKGMTYV--DCHKQFQRCVNELKQS 100

[Close]

BLAST of Cucsa.281890 vs. Swiss-Prot
Match: PLA21_ORYSJ (Probable phospholipase A2 homolog 1 OS=Oryza sativa subsp. japonica GN=PLA2-I PE=2 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 9.2e-16
Identity = 48/135 (35.56%), Postives = 71/135 (52.59%), Query Frame = 1


Query: 10  LPSIVFLPSHALNIGVQALDASVTMSKDCSRKCESEFCSVPPLLRYGKYCGLLYSGCPGE 69
           L ++VFL +  L+       A+      CSR C +  C    + RYGKYCG+ +SGC GE
Sbjct: 7   LLALVFLAAGVLS------SATSPPPPPCSRSCAALNCDSVGI-RYGKYCGVGWSGCDGE 66

Query: 70  HPCDGLDACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRSRSSPFKGNKCQVDEVIE 129
            PCD LDACC  HD CV  K    +S +C + F NCM   K++    F   KC  +  + 
Sbjct: 67  EPCDDLDACCRDHDHCVDKKG--LMSVKCHEKFKNCMRKVKKAGKIGF-SRKCPYEMAMA 126

Query: 130 VISLVMEAALIAGRV 145
            ++  M+ A++  ++
Sbjct: 127 TMTSGMDMAIMLSQL 131

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BLAST of Cucsa.281890 vs. TrEMBL
Match: A0A0A0LUV2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G433610 PE=3 SV=1)

HSP 1 Score: 311.2 bits (796), Expect = 6.7e-82
Identity = 148/148 (100.00%), Postives = 148/148 (100.00%), Query Frame = 1


Query: 1   MPQFLLLLLLPSIVFLPSHALNIGVQALDASVTMSKDCSRKCESEFCSVPPLLRYGKYCG 60
           MPQFLLLLLLPSIVFLPSHALNIGVQALDASVTMSKDCSRKCESEFCSVPPLLRYGKYCG
Sbjct: 1   MPQFLLLLLLPSIVFLPSHALNIGVQALDASVTMSKDCSRKCESEFCSVPPLLRYGKYCG 60

Query: 61  LLYSGCPGEHPCDGLDACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRSRSSPFKGN 120
           LLYSGCPGEHPCDGLDACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRSRSSPFKGN
Sbjct: 61  LLYSGCPGEHPCDGLDACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRSRSSPFKGN 120

Query: 121 KCQVDEVIEVISLVMEAALIAGRVFHKP 149
           KCQVDEVIEVISLVMEAALIAGRVFHKP
Sbjct: 121 KCQVDEVIEVISLVMEAALIAGRVFHKP 148

[Close]

BLAST of Cucsa.281890 vs. TrEMBL
Match: A0A067K6H4_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_20247 PE=3 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 3.2e-60
Identity = 108/133 (81.20%), Postives = 119/133 (89.47%), Query Frame = 1


Query: 16  LPSHALNIGVQALDASVTMSKDCSRKCESEFCSVPPLLRYGKYCGLLYSGCPGEHPCDGL 75
           +P HALNIGVQ  DASV++SK+CSRKCESEFCSVPP LRYGKYCGLLYSGCPGE PCDGL
Sbjct: 24  VPIHALNIGVQRADASVSVSKECSRKCESEFCSVPPFLRYGKYCGLLYSGCPGERPCDGL 83

Query: 76  DACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRSRSSPFKGNKCQVDEVIEVISLVM 135
           DACCMKHDACV AKN+ YLSQECSQ+F+NCM NFK +    FKGNKCQVDEVI++ISLVM
Sbjct: 84  DACCMKHDACVQAKNNGYLSQECSQNFINCMTNFKNTGGHTFKGNKCQVDEVIDIISLVM 143

Query: 136 EAALIAGRVFHKP 149
           EAAL+AGR FHKP
Sbjct: 144 EAALLAGRYFHKP 156

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BLAST of Cucsa.281890 vs. TrEMBL
Match: D7UBL5_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_11s0103g00190 PE=3 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 3.2e-60
Identity = 113/146 (77.40%), Postives = 125/146 (85.62%), Query Frame = 1


Query: 5   LLLLLLPSIVF--LPSHALNIGVQALDASVTMSKDCSRKCESEFCSVPPLLRYGKYCGLL 64
           LLL  L  ++F   P+ ALNIGVQA D SVT+SK+CSRKCESEFCSVPP LRYGKYCGLL
Sbjct: 3   LLLCSLIGLIFSATPTLALNIGVQATDGSVTLSKECSRKCESEFCSVPPFLRYGKYCGLL 62

Query: 65  YSGCPGEHPCDGLDACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRSRSSPFKGNKC 124
           YSGCPGE PCDGLDACCMKHDACV AKN+DYLSQECSQ+F+NCM +FK S    FKGNKC
Sbjct: 63  YSGCPGEKPCDGLDACCMKHDACVQAKNNDYLSQECSQNFINCMNSFKSSGGHTFKGNKC 122

Query: 125 QVDEVIEVISLVMEAALIAGRVFHKP 149
           QVDEVI+VI+LVMEAAL+AGR  HKP
Sbjct: 123 QVDEVIDVITLVMEAALLAGRYLHKP 148

[Close]

BLAST of Cucsa.281890 vs. TrEMBL
Match: A0A061GRE9_THECC (Phospholipase A21 OS=Theobroma cacao GN=TCM_039586 PE=3 SV=1)

HSP 1 Score: 236.5 bits (602), Expect = 2.1e-59
Identity = 108/149 (72.48%), Postives = 129/149 (86.58%), Query Frame = 1


Query: 3   QFLLL---LLLPSIVFLPSHALNIGVQALDASVTMSKDCSRKCESEFCSVPPLLRYGKYC 62
           QFLL    ++  ++  +P  ALN+GVQA D+++T+SK+CSRKCESEFCSVPP LRYGKYC
Sbjct: 5   QFLLFSFSVIAFTLSSVPVEALNVGVQAADSAITVSKECSRKCESEFCSVPPFLRYGKYC 64

Query: 63  GLLYSGCPGEHPCDGLDACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRSRSSPFKG 122
           GLLYSGCPGE PCDGLDACCMKHDACV AKN+DYLSQECSQ+F+NCM NF++S    FKG
Sbjct: 65  GLLYSGCPGEKPCDGLDACCMKHDACVQAKNNDYLSQECSQNFINCMTNFQKSGGRTFKG 124

Query: 123 NKCQVDEVIEVISLVMEAALIAGRVFHKP 149
           NKCQVDEVI+VI++VM+AAL+AGRV HKP
Sbjct: 125 NKCQVDEVIDVITVVMDAALLAGRVLHKP 153

[Close]

BLAST of Cucsa.281890 vs. TrEMBL
Match: B9SCE0_RICCO (Phospholipase A21 OS=Ricinus communis GN=RCOM_0893800 PE=3 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 4.7e-59
Identity = 109/145 (75.17%), Postives = 123/145 (84.83%), Query Frame = 1


Query: 4   FLLLLLLPSIVFLPSHALNIGVQALDASVTMSKDCSRKCESEFCSVPPLLRYGKYCGLLY 63
           FLL L  PS    P HALNIGVQ  ++++T+SK+CSRKCESEFCSVPP LRYGKYCGLLY
Sbjct: 19  FLLSLSFPST---PVHALNIGVQTANSAITLSKECSRKCESEFCSVPPFLRYGKYCGLLY 78

Query: 64  SGCPGEHPCDGLDACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRSRSSPFKGNKCQ 123
           SGCPGE PCDGLDACCMKHD+CV AKN+DYLSQECSQ+F+NCM +FK      FKG+KCQ
Sbjct: 79  SGCPGEKPCDGLDACCMKHDSCVQAKNNDYLSQECSQNFINCMNDFKNKGGHTFKGSKCQ 138

Query: 124 VDEVIEVISLVMEAALIAGRVFHKP 149
           VDEVI+VIS+VMEAALIAGR  HKP
Sbjct: 139 VDEVIDVISVVMEAALIAGRYLHKP 160

[Close]

BLAST of Cucsa.281890 vs. TAIR10
Match: AT2G06925.1 (AT2G06925.1 Phospholipase A2 family protein)

HSP 1 Score: 220.7 bits (561), Expect = 6.0e-58
Identity = 96/129 (74.42%), Postives = 112/129 (86.82%), Query Frame = 1


Query: 20  ALNIGVQALDASVTMSKDCSRKCESEFCSVPPLLRYGKYCGLLYSGCPGEHPCDGLDACC 79
           ALN+GVQ +  S++++K+CSRKCESEFCSVPP LRYGKYCGLLYSGCPGE PCDGLD+CC
Sbjct: 20  ALNVGVQLIHPSISLTKECSRKCESEFCSVPPFLRYGKYCGLLYSGCPGERPCDGLDSCC 79

Query: 80  MKHDACVVAKNDDYLSQECSQSFLNCMENFKRSRSSPFKGNKCQVDEVIEVISLVMEAAL 139
           MKHDACV +KN+DYLSQECSQ F+NCM NF + +   FKGNKC  DEVI+VIS+VMEAAL
Sbjct: 80  MKHDACVQSKNNDYLSQECSQKFINCMNNFSQKKQPTFKGNKCDADEVIDVISIVMEAAL 139

Query: 140 IAGRVFHKP 149
           IAG+V  KP
Sbjct: 140 IAGKVLKKP 148

[Close]

BLAST of Cucsa.281890 vs. TAIR10
Match: AT4G29470.1 (AT4G29470.1 Phospholipase A2 family protein)

HSP 1 Score: 86.3 bits (212), Expect = 1.8e-17
Identity = 43/93 (46.24%), Postives = 56/93 (60.22%), Query Frame = 1


Query: 20  ALNIGVQALDASVTMSKDCSRKCESEFCSVPPLLRYGKYCGLLYSGCPGEHPCDGLDACC 79
           AL + V  L A V   + CS+ C ++ C+V   +RYGKYCG+ Y GCPGE PCD LD CC
Sbjct: 11  ALGLTVFLLLAVVHSQEKCSKTCIAQKCNVLG-IRYGKYCGIGYFGCPGEPPCDDLDDCC 70

Query: 80  MKHDACVVAKNDDYLSQECSQSFLNCMENFKRS 113
           M HD CV  K   Y+  +C + F  C+   K+S
Sbjct: 71  MTHDNCVDLKGMTYV--DCHKQFQRCVNELKQS 100

[Close]

BLAST of Cucsa.281890 vs. TAIR10
Match: AT4G29460.1 (AT4G29460.1 Phospholipase A2 family protein)

HSP 1 Score: 81.6 bits (200), Expect = 4.4e-16
Identity = 38/83 (45.78%), Postives = 50/83 (60.24%), Query Frame = 1


Query: 30  ASVTMSKDCSRKCESEFCSVPPLLRYGKYCGLLYSGCPGEHPCDGLDACCMKHDACVVAK 89
           A V+  + CS  C ++ C+   + RYGKYCG+ Y GCPGE PCD LDACCM HD CV  K
Sbjct: 21  AVVSSQEKCSNTCIAQNCNSLGI-RYGKYCGIGYFGCPGEPPCDDLDACCMTHDNCVDLK 80

Query: 90  NDDYLSQECSQSFLNCMENFKRS 113
              Y++  C + F  C+    +S
Sbjct: 81  GMTYVN--CHKQFKRCVNKLSKS 100

[Close]

BLAST of Cucsa.281890 vs. TAIR10
Match: AT2G19690.2 (AT2G19690.2 phospholipase A2-beta)

HSP 1 Score: 80.9 bits (198), Expect = 7.4e-16
Identity = 43/122 (35.25%), Postives = 66/122 (54.10%), Query Frame = 1


Query: 32  VTMSKDCSRKCESEFCSVPPLLRYGKYCGLLYSGCPGEHPCDGLDACCMKHDACVVAKND 91
           V  S++C+R C ++ C    + RYGKYCG+ +SGCPGE PCD LDACC  HD CV     
Sbjct: 25  VARSEECTRTCIAQNCDTLSI-RYGKYCGIGHSGCPGEEPCDDLDACCKIHDHCVELNGM 84

Query: 92  DYLSQECSQSFLNCMENFKRS-RSSPFK----GNKCQVDEVIEVISLVMEAALIAGRV-F 148
             +S  C + F  C+    ++ + S  K      KC    VI  ++  M+  ++  ++ +
Sbjct: 85  TNIS--CHKKFQRCVNRLSKAIKQSKNKKVGFSTKCPYSVVIPTVNQGMDIGILFSQLAY 143

[Close]

BLAST of Cucsa.281890 vs. NCBI nr
Match: gi|449466059|ref|XP_004150744.1| (PREDICTED: phospholipase A2-alpha [Cucumis sativus])

HSP 1 Score: 311.2 bits (796), Expect = 9.6e-82
Identity = 148/148 (100.00%), Postives = 148/148 (100.00%), Query Frame = 1


Query: 1   MPQFLLLLLLPSIVFLPSHALNIGVQALDASVTMSKDCSRKCESEFCSVPPLLRYGKYCG 60
           MPQFLLLLLLPSIVFLPSHALNIGVQALDASVTMSKDCSRKCESEFCSVPPLLRYGKYCG
Sbjct: 1   MPQFLLLLLLPSIVFLPSHALNIGVQALDASVTMSKDCSRKCESEFCSVPPLLRYGKYCG 60

Query: 61  LLYSGCPGEHPCDGLDACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRSRSSPFKGN 120
           LLYSGCPGEHPCDGLDACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRSRSSPFKGN
Sbjct: 61  LLYSGCPGEHPCDGLDACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRSRSSPFKGN 120

Query: 121 KCQVDEVIEVISLVMEAALIAGRVFHKP 149
           KCQVDEVIEVISLVMEAALIAGRVFHKP
Sbjct: 121 KCQVDEVIEVISLVMEAALIAGRVFHKP 148

[Close]

BLAST of Cucsa.281890 vs. NCBI nr
Match: gi|659107123|ref|XP_008453533.1| (PREDICTED: phospholipase A2-alpha [Cucumis melo])

HSP 1 Score: 293.1 bits (749), Expect = 2.7e-76
Identity = 141/156 (90.38%), Postives = 144/156 (92.31%), Query Frame = 1


Query: 1   MPQFLLLLLL--------PSIVFLPSHALNIGVQALDASVTMSKDCSRKCESEFCSVPPL 60
           MPQFLLLLLL        PSI FLPSHALNIGVQALDAS+TMSKDCSRKCESEFCSVPPL
Sbjct: 1   MPQFLLLLLLLLLLLLLLPSIAFLPSHALNIGVQALDASITMSKDCSRKCESEFCSVPPL 60

Query: 61  LRYGKYCGLLYSGCPGEHPCDGLDACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRS 120
           LRYGKYCGLLYSGCPGE PCDGLDACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRS
Sbjct: 61  LRYGKYCGLLYSGCPGERPCDGLDACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRS 120

Query: 121 RSSPFKGNKCQVDEVIEVISLVMEAALIAGRVFHKP 149
           RS PFKGNKCQVDEVI+VIS VMEAALIAG+VFHKP
Sbjct: 121 RSRPFKGNKCQVDEVIDVISFVMEAALIAGKVFHKP 156

[Close]

BLAST of Cucsa.281890 vs. NCBI nr
Match: gi|802710031|ref|XP_012084568.1| (PREDICTED: phospholipase A2-alpha [Jatropha curcas])

HSP 1 Score: 239.2 bits (609), Expect = 4.6e-60
Identity = 108/133 (81.20%), Postives = 119/133 (89.47%), Query Frame = 1


Query: 16  LPSHALNIGVQALDASVTMSKDCSRKCESEFCSVPPLLRYGKYCGLLYSGCPGEHPCDGL 75
           +P HALNIGVQ  DASV++SK+CSRKCESEFCSVPP LRYGKYCGLLYSGCPGE PCDGL
Sbjct: 24  VPIHALNIGVQRADASVSVSKECSRKCESEFCSVPPFLRYGKYCGLLYSGCPGERPCDGL 83

Query: 76  DACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRSRSSPFKGNKCQVDEVIEVISLVM 135
           DACCMKHDACV AKN+ YLSQECSQ+F+NCM NFK +    FKGNKCQVDEVI++ISLVM
Sbjct: 84  DACCMKHDACVQAKNNGYLSQECSQNFINCMTNFKNTGGHTFKGNKCQVDEVIDIISLVM 143

Query: 136 EAALIAGRVFHKP 149
           EAAL+AGR FHKP
Sbjct: 144 EAALLAGRYFHKP 156

[Close]

BLAST of Cucsa.281890 vs. NCBI nr
Match: gi|225446293|ref|XP_002269830.1| (PREDICTED: phospholipase A2-alpha [Vitis vinifera])

HSP 1 Score: 239.2 bits (609), Expect = 4.6e-60
Identity = 113/146 (77.40%), Postives = 125/146 (85.62%), Query Frame = 1


Query: 5   LLLLLLPSIVF--LPSHALNIGVQALDASVTMSKDCSRKCESEFCSVPPLLRYGKYCGLL 64
           LLL  L  ++F   P+ ALNIGVQA D SVT+SK+CSRKCESEFCSVPP LRYGKYCGLL
Sbjct: 7   LLLCSLIGLIFSATPTLALNIGVQATDGSVTLSKECSRKCESEFCSVPPFLRYGKYCGLL 66

Query: 65  YSGCPGEHPCDGLDACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRSRSSPFKGNKC 124
           YSGCPGE PCDGLDACCMKHDACV AKN+DYLSQECSQ+F+NCM +FK S    FKGNKC
Sbjct: 67  YSGCPGEKPCDGLDACCMKHDACVQAKNNDYLSQECSQNFINCMNSFKSSGGHTFKGNKC 126

Query: 125 QVDEVIEVISLVMEAALIAGRVFHKP 149
           QVDEVI+VI+LVMEAAL+AGR  HKP
Sbjct: 127 QVDEVIDVITLVMEAALLAGRYLHKP 152

[Close]

BLAST of Cucsa.281890 vs. NCBI nr
Match: gi|296090311|emb|CBI40130.3| (unnamed protein product [Vitis vinifera])

HSP 1 Score: 239.2 bits (609), Expect = 4.6e-60
Identity = 113/146 (77.40%), Postives = 125/146 (85.62%), Query Frame = 1


Query: 5   LLLLLLPSIVF--LPSHALNIGVQALDASVTMSKDCSRKCESEFCSVPPLLRYGKYCGLL 64
           LLL  L  ++F   P+ ALNIGVQA D SVT+SK+CSRKCESEFCSVPP LRYGKYCGLL
Sbjct: 3   LLLCSLIGLIFSATPTLALNIGVQATDGSVTLSKECSRKCESEFCSVPPFLRYGKYCGLL 62

Query: 65  YSGCPGEHPCDGLDACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRSRSSPFKGNKC 124
           YSGCPGE PCDGLDACCMKHDACV AKN+DYLSQECSQ+F+NCM +FK S    FKGNKC
Sbjct: 63  YSGCPGEKPCDGLDACCMKHDACVQAKNNDYLSQECSQNFINCMNSFKSSGGHTFKGNKC 122

Query: 125 QVDEVIEVISLVMEAALIAGRVFHKP 149
           QVDEVI+VI+LVMEAAL+AGR  HKP
Sbjct: 123 QVDEVIDVITLVMEAALLAGRYLHKP 148

The following BLAST results are available for this feature:

BLAST of Cucsa.281890 vs. Swiss-Prot


Match Name	E-value	Identity	Description
PLA2A_ARATH	1.1e-56	74.42	Phospholipase A2-alpha OS=Arabidopsis thaliana GN=PLA2-ALPHA PE=1 SV=1	[more]
PLA23_ORYSJ	9.4e-45	60.99	Phospholipase A2 homolog 3 OS=Oryza sativa subsp. japonica GN=PLA2-III PE=1 SV=1	[more]
PLA22_ORYSJ	7.2e-37	50.68	Probable phospholipase A2 homolog 2 OS=Oryza sativa subsp. japonica GN=PLA2-II P...	[more]
PLA2D_ARATH	3.1e-16	46.24	Phospholipase A2-delta OS=Arabidopsis thaliana GN=PLA2-DELTA PE=2 SV=1	[more]
PLA21_ORYSJ	9.2e-16	35.56	Probable phospholipase A2 homolog 1 OS=Oryza sativa subsp. japonica GN=PLA2-I PE...	[more]

BLAST of Cucsa.281890 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LUV2_CUCSA	6.7e-82	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G433610 PE=3 SV=1	[more]
A0A067K6H4_JATCU	3.2e-60	81.20	Uncharacterized protein OS=Jatropha curcas GN=JCGZ_20247 PE=3 SV=1	[more]
D7UBL5_VITVI	3.2e-60	77.40	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_11s0103g00190 PE=3 SV=...	[more]
A0A061GRE9_THECC	2.1e-59	72.48	Phospholipase A21 OS=Theobroma cacao GN=TCM_039586 PE=3 SV=1	[more]
B9SCE0_RICCO	4.7e-59	75.17	Phospholipase A21 OS=Ricinus communis GN=RCOM_0893800 PE=3 SV=1	[more]

BLAST of Cucsa.281890 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G06925.1	6.0e-58	74.42	Phospholipase A2 family protein	[more]
AT4G29470.1	1.8e-17	46.24	Phospholipase A2 family protein	[more]
AT4G29460.1	4.4e-16	45.78	Phospholipase A2 family protein	[more]
AT2G19690.2	7.4e-16	35.25	phospholipase A2-beta	[more]

BLAST of Cucsa.281890 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449466059\|ref\|XP_004150744.1\|	9.6e-82	100.00	PREDICTED: phospholipase A2-alpha [Cucumis sativus]	[more]
gi\|659107123\|ref\|XP_008453533.1\|	2.7e-76	90.38	PREDICTED: phospholipase A2-alpha [Cucumis melo]	[more]
gi\|802710031\|ref\|XP_012084568.1\|	4.6e-60	81.20	PREDICTED: phospholipase A2-alpha [Jatropha curcas]	[more]
gi\|225446293\|ref\|XP_002269830.1\|	4.6e-60	77.40	PREDICTED: phospholipase A2-alpha [Vitis vinifera]	[more]
gi\|296090311\|emb\|CBI40130.3\|	4.6e-60	77.40	unnamed protein product [Vitis vinifera]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR001211	PLipase_A2
IPR016090	Phospholipase A2 domain

Vocabulary: **Molecular Function**
Term	Definition
GO:0004623	phospholipase A2 activity
GO:0005509	calcium ion binding

Vocabulary: **Biological Process**
Term	Definition
GO:0016042	lipid catabolic process

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009395	phospholipid catabolic process
biological_process	GO:0006644	phospholipid metabolic process
biological_process	GO:0016042	lipid catabolic process
biological_process	GO:0050482	arachidonic acid secretion
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0005794	Golgi apparatus
cellular_component	GO:0005773	vacuole
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0004623	phospholipase A2 activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsa.281890.1	Cucsa.281890.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001211	Phospholipase A2	PANTHER	PTHR11716	PHOSPHOLIPASE A2	coord: 37..148 score: 1.2E-70coord: 3..18 score: 1.2
IPR016090	Phospholipase A2 domain	GENE3D	G3DSA:1.20.90.10		coord: 42..110 score: 9.6
IPR016090	Phospholipase A2 domain	unknown	SSF48619	Phospholipase A2, PLA2	coord: 53..147 score: 3.28
None	No IPR available	PANTHER	PTHR11716:SF54	PHOSPHOLIPASE A2-ALPHA	coord: 3..18 score: 1.2E-70coord: 37..148 score: 1.2

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cucsa.281890	Cla018886	Watermelon (97103) v1	cgywmB517
Cucsa.281890	Csa1G433610	Cucumber (Chinese Long) v2	cgycuB401
Cucsa.281890	MELO3C017400	Melon (DHL92) v3.5.1	cgymeB471
Cucsa.281890	ClCG06G013940	Watermelon (Charleston Gray)	cgywcgB488
Cucsa.281890	CSPI01G20830	Wild cucumber (PI 183967)	cgycpiB424
Cucsa.281890	CmaCh11G003380	Cucurbita maxima (Rimu)	cgycmaB0786
Cucsa.281890	CmoCh11G003410	Cucurbita moschata (Rifu)	cgycmoB0781
Cucsa.281890	Cp4.1LG04g13510	Cucurbita pepo (Zucchini)	cgycpeB0743
Cucsa.281890	MELO3C017400.2	Melon (DHL92) v3.6.1	cgymedB468
Cucsa.281890	CsaV3_1G032150	Cucumber (Chinese Long) v3	cgycucB432
Cucsa.281890	Cla97C06G123370	Watermelon (97103) v2	cgywmbB491
Cucsa.281890	Bhi02G000143	Wax gourd	cgywgoB570
Cucsa.281890	Carg09075	Silver-seed gourd	carcgyB0675

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cucsa.281890	Cucurbita maxima (Rimu)	cgycmaB0785
Cucsa.281890	Cucurbita moschata (Rifu)	cgycmoB0780
Cucsa.281890	Cucurbita pepo (Zucchini)	cgycpeB0741
Cucsa.281890	Bottle gourd (USVL1VR-Ls)	cgylsiB457
Cucsa.281890	Silver-seed gourd	carcgyB0900