Cucsa.279470 (gene) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.279470
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	Outward rectifying potassium channel protein
Location	scaffold02622 : 60783 .. 62134 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TGTTGAATTATCTTAATGGGTAGCCAAGATGCTAGACAACCACTGTTACCAACATCATCTAATACTTTAGAAACAAGGGTAATAAATATTCCTAGAAGCAAAAGGCGATTGAGGCGCACCAAGAGTGCTCCTCATGCAAATTCTCCTACTGAGATCACCCACACCAGCAACGTGCCGGCCACAGGTCCAGTTCCACGTTCAGGATTAATATTCGGAAATCTACACCCGAGTTTCAGAAGAGTAGCCTTAGTTTTAATAACATACCTGGGTATAGGAACTTTATGTTTTTACCTTGTCAGGCACCAAATCCAAGGGGAGAAAACAAACAGATTAGTTGATGCCATATATTTCACTATTGTGACAATGACAACTGTTGGGTATGGAGATCTTGTCCCAAACAGTCCTTCCACAAAACTGCTAGCTTGTGCTTTCGTTTTCACAGGAATGGCCCTAGTTGGCCTAATACTGAGCAATGCAGCCGATTATTTGGTAGAGAAGCAGGAAATCTTGCTATTTAAAGCCTTCCATATTGACCAAAATGGCCATTGTGACATTTCGAAAGAAATCGATACTAATAAAGCTAGAAACAAGTGCATAGTGGTGTTTCTCCTTCTTCTTTTGTTCATCATTTCTGGGACTGCCTTTCTAGTTACCATTGAGAAGTTAGATTTTATTGATGCCTTTTATTGTGTATGTTCTACGATCACAACGCTAGGCTATGGAGATCAAAGCTTTTCAACCAAGTGGGGACGTGTGTTTGCCATTTTCTGGATCTTGATAAGTACCATCACTCTAGCTCAGTTTTTCCTTTACATTGCTGAGCTAAACACTGAAAGAAGGCAAAAGTCTCTTGTAAAGTGGGTTCTAAGTAAAAAGGTGACAGACATAGACCTTGAGGTTGCAGATATCGATGATGACGGTGTTGTCGGGTAAGTCATTGCCTATTCTTTTCATTACACTCCCCTATCATGTAATCGCAAATTCATTTGATCAGAGCACATCCATTCCTTTCTGATCAGACCTCTAGCATTTTTGTCAATTTCGTAGTTGAAGTTGATTGAATTTCTATAGCTATTTGCTTATTGTTCCTTCTTAATCTTCCACAGGGCTGCAGAATTTGTGATATACAAGCTGAAGGAGATGGGGAAGATTACTGAAGATGACATTTCACTTGTGTTGAATGAATTTGAGAATCTTGATGTTGATCAATCAGGAACACTGTCCATATCTGATATAACACTTGCTCAATTATCTTAAGCAGAGATGATGATGGTGGCCTTTCTTCTATGACATCAGTTAAGTTGTTTTTGCAATGTTTTTATATTGCTCGACTTTCTGCTTCTTATCTTGTG

mRNA sequence

TGTTGAATTATCTTAATGGGTAGCCAAGATGCTAGACAACCACTGTTACCAACATCATCTAATACTTTAGAAACAAGGGTAATAAATATTCCTAGAAGCAAAAGGCGATTGAGGCGCACCAAGAGTGCTCCTCATGCAAATTCTCCTACTGAGATCACCCACACCAGCAACGTGCCGGCCACAGGTCCAGTTCCACGTTCAGGATTAATATTCGGAAATCTACACCCGAGTTTCAGAAGAGTAGCCTTAGTTTTAATAACATACCTGGGTATAGGAACTTTATGTTTTTACCTTGTCAGGCACCAAATCCAAGGGGAGAAAACAAACAGATTAGTTGATGCCATATATTTCACTATTGTGACAATGACAACTGTTGGGTATGGAGATCTTGTCCCAAACAGTCCTTCCACAAAACTGCTAGCTTGTGCTTTCGTTTTCACAGGAATGGCCCTAGTTGGCCTAATACTGAGCAATGCAGCCGATTATTTGGTAGAGAAGCAGGAAATCTTGCTATTTAAAGCCTTCCATATTGACCAAAATGGCCATTGTGACATTTCGAAAGAAATCGATACTAATAAAGCTAGAAACAAGTGCATAGTGGTGTTTCTCCTTCTTCTTTTGTTCATCATTTCTGGGACTGCCTTTCTAGTTACCATTGAGAAGTTAGATTTTATTGATGCCTTTTATTGTGTATGTTCTACGATCACAACGCTAGGCTATGGAGATCAAAGCTTTTCAACCAAGTGGGGACGTGTGTTTGCCATTTTCTGGATCTTGATAAGTACCATCACTCTAGCTCAGTTTTTCCTTTACATTGCTGAGCTAAACACTGAAAGAAGGCAAAAGTCTCTTGTAAAGTGGGTTCTAAGTAAAAAGGTGACAGACATAGACCTTGAGGTTGCAGATATCGATGATGACGGTGTTGTCGGGGCTGCAGAATTTGTGATATACAAGCTGAAGGAGATGGGGAAGATTACTGAAGATGACATTTCACTTGTGTTGAATGAATTTGAGAATCTTGATGTTGATCAATCAGGAACACTGTCCATATCTGATATAACACTTGCTCAATTATCTTAAGCAGAGATGATGATGGTGGCCTTTCTTCTATGACATCAGTTAAGTTGTTTTTGCAATGTTTTTATATTGCTCGACTTTCTGCTTCTTATCTTGTG

Coding sequence (CDS)

ATGGGTAGCCAAGATGCTAGACAACCACTGTTACCAACATCATCTAATACTTTAGAAACAAGGGTAATAAATATTCCTAGAAGCAAAAGGCGATTGAGGCGCACCAAGAGTGCTCCTCATGCAAATTCTCCTACTGAGATCACCCACACCAGCAACGTGCCGGCCACAGGTCCAGTTCCACGTTCAGGATTAATATTCGGAAATCTACACCCGAGTTTCAGAAGAGTAGCCTTAGTTTTAATAACATACCTGGGTATAGGAACTTTATGTTTTTACCTTGTCAGGCACCAAATCCAAGGGGAGAAAACAAACAGATTAGTTGATGCCATATATTTCACTATTGTGACAATGACAACTGTTGGGTATGGAGATCTTGTCCCAAACAGTCCTTCCACAAAACTGCTAGCTTGTGCTTTCGTTTTCACAGGAATGGCCCTAGTTGGCCTAATACTGAGCAATGCAGCCGATTATTTGGTAGAGAAGCAGGAAATCTTGCTATTTAAAGCCTTCCATATTGACCAAAATGGCCATTGTGACATTTCGAAAGAAATCGATACTAATAAAGCTAGAAACAAGTGCATAGTGGTGTTTCTCCTTCTTCTTTTGTTCATCATTTCTGGGACTGCCTTTCTAGTTACCATTGAGAAGTTAGATTTTATTGATGCCTTTTATTGTGTATGTTCTACGATCACAACGCTAGGCTATGGAGATCAAAGCTTTTCAACCAAGTGGGGACGTGTGTTTGCCATTTTCTGGATCTTGATAAGTACCATCACTCTAGCTCAGTTTTTCCTTTACATTGCTGAGCTAAACACTGAAAGAAGGCAAAAGTCTCTTGTAAAGTGGGTTCTAAGTAAAAAGGTGACAGACATAGACCTTGAGGTTGCAGATATCGATGATGACGGTGTTGTCGGGGCTGCAGAATTTGTGATATACAAGCTGAAGGAGATGGGGAAGATTACTGAAGATGACATTTCACTTGTGTTGAATGAATTTGAGAATCTTGATGTTGATCAATCAGGAACACTGTCCATATCTGATATAACACTTGCTCAATTATCTTAA

Protein sequence

MGSQDARQPLLPTSSNTLETRVINIPRSKRRLRRTKSAPHANSPTEITHTSNVPATGPVPRSGLIFGNLHPSFRRVALVLITYLGIGTLCFYLVRHQIQGEKTNRLVDAIYFTIVTMTTVGYGDLVPNSPSTKLLACAFVFTGMALVGLILSNAADYLVEKQEILLFKAFHIDQNGHCDISKEIDTNKARNKCIVVFLLLLLFIISGTAFLVTIEKLDFIDAFYCVCSTITTLGYGDQSFSTKWGRVFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWVLSKKVTDIDLEVADIDDDGVVGAAEFVIYKLKEMGKITEDDISLVLNEFENLDVDQSGTLSISDITLAQLS*

Homology

[Close]

BLAST of Cucsa.279470 vs. Swiss-Prot
Match: KCO1_ARATH (Two-pore potassium channel 1 OS=Arabidopsis thaliana GN=TPK1 PE=1 SV=2)

HSP 1 Score: 408.3 bits (1048), Expect = 8.6e-113
Identity = 213/360 (59.17%), Postives = 267/360 (74.17%), Query Frame = 1


Query: 1   MGSQDARQPLLPTSSNTLETRVINI-----PRSKRRLRRTKSAPHANSPTEITHTSNVPA 60
           M S  AR PLLPT       +  N+        KRRLRR++SAP      +  +  +V  
Sbjct: 1   MSSDAARTPLLPTEKIDTMAQDFNLNSRTSSSRKRRLRRSRSAPRG----DCMYNDDVKI 60

Query: 61  TGPVPRSGLI--FGNLHPSFRRVALVLITYLGIGTLCFYLVRHQIQGEKTNRLVDAIYFT 120
             P P    I  F +L+P+ RRV + L  YL IGTLCFYLVR QI G KT+ +VDA+YF 
Sbjct: 61  DEPPPHPSKIPMFSDLNPNLRRVIMFLALYLTIGTLCFYLVRDQISGHKTSGVVDALYFC 120

Query: 121 IVTMTTVGYGDLVPNSPSTKLLACAFVFTGMALVGLILSNAADYLVEKQEILLFKAFHID 180
           IVTMTTVGYGDLVPNS +++LLACAFVF+GM LVG +LS AADYLVEKQE LL +AFH+ 
Sbjct: 121 IVTMTTVGYGDLVPNSSASRLLACAFVFSGMVLVGHLLSRAADYLVEKQEALLVRAFHLR 180

Query: 181 QN-GHCDISKEIDTNKARNKCIVVFLLLLLFIISGTAFLVTIEKLDFIDAFYCVCSTITT 240
           Q+ G  DI KE+ TNK R KC    L+L++  I GT FLV +EK+  I AFYCVCST+TT
Sbjct: 181 QSFGPTDILKELHTNKLRYKCYATCLVLVVLFIVGTIFLVMVEKMPVISAFYCVCSTVTT 240

Query: 241 LGYGDQSFSTKWGRVFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWVLSKKVTDID 300
           LGYGD+SF+++ GR+FA+FWIL S+I LAQFFLY+AELNTE +Q++LVKWVL++++T+ D
Sbjct: 241 LGYGDKSFNSEAGRLFAVFWILTSSICLAQFFLYVAELNTENKQRALVKWVLTRRITNND 300

Query: 301 LEVADIDDDGVVGAAEFVIYKLKEMGKITEDDISLVLNEFENLDVDQSGTLSISDITLAQ 353
           LE AD+D+DGVVGAAEF++YKLKEMGKI E DIS +++EFE LD D+SGTL+ SDI LAQ
Sbjct: 301 LEAADLDEDGVVGAAEFIVYKLKEMGKIDEKDISGIMDEFEQLDYDESGTLTTSDIVLAQ 356

[Close]

BLAST of Cucsa.279470 vs. Swiss-Prot
Match: KCO1_ORYSJ (Two pore potassium channel a OS=Oryza sativa subsp. japonica GN=TPKA PE=1 SV=1)

HSP 1 Score: 371.3 bits (952), Expect = 1.2e-101
Identity = 193/353 (54.67%), Postives = 246/353 (69.69%), Query Frame = 1


Query: 1   MGSQDARQPLLPTSSNTLETRVINIPRSKRRLRRTKSAPHANSPTEITHTSNVPATGPVP 60
           M     +Q LL  + N L+ +        RR R T S      P         P  G   
Sbjct: 1   MDDNSIQQSLLADNPNVLQRKPSEGVNRFRRCRSTPSTDPLQGP---------PEKGSSV 60

Query: 61  RSGLIFGNLHPSFRRVALVLITYLGIGTLCFYLVRHQIQGEKTNRLVDAIYFTIVTMTTV 120
           ++  +F  + PSFR V L+L  YL +G L FY V  +I G++TNR++DA+YF +VTMTTV
Sbjct: 61  KAKELFKEMRPSFRLVGLLLFIYLLVGVLAFYAVMDEISGKRTNRVLDALYFCVVTMTTV 120

Query: 121 GYGDLVPNSPSTKLLACAFVFTGMALVGLILSNAADYLVEKQEILLFKAFHID-QNGHCD 180
           GYGDLVPN+ +TKLLACAFVF GMA+V L +S  ADYLVEKQE+L FKA H + + G   
Sbjct: 121 GYGDLVPNNDTTKLLACAFVFMGMAVVALFVSKVADYLVEKQEVLFFKALHTNLKGGETK 180

Query: 181 ISKEIDTNKARNKCIVVFLLLLLFIISGTAFLVTIEKLDFIDAFYCVCSTITTLGYGDQS 240
           + + I+TN+ + K     LLL+L IISGT FL  +EKL  +D+FYCVC+TITTLGYGD+S
Sbjct: 181 MLRAIETNRIKYKFYTNALLLVLSIISGTVFLWKVEKLSLVDSFYCVCATITTLGYGDKS 240

Query: 241 FSTKWGRVFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWVLSKKVTDIDLEVADID 300
           FS+K GRVFA+FWI+ STI +AQFF+Y+AE+ TERRQK L  WVL++K+T +DLE AD+D
Sbjct: 241 FSSKLGRVFAVFWIITSTIIMAQFFMYLAEIYTERRQKMLANWVLTRKMTKMDLEAADLD 300

Query: 301 DDGVVGAAEFVIYKLKEMGKITEDDISLVLNEFENLDVDQSGTLSISDITLAQ 353
           DD  VGAAEFV+YKLKE+GKI +++IS  L EFE LDVD SGTLS  D+TLAQ
Sbjct: 301 DDRQVGAAEFVVYKLKELGKINQEEISSFLEEFEKLDVDHSGTLSPYDLTLAQ 344

[Close]

BLAST of Cucsa.279470 vs. Swiss-Prot
Match: KCO2_ORYSJ (Two pore potassium channel b OS=Oryza sativa subsp. japonica GN=TPKB PE=1 SV=1)

HSP 1 Score: 335.5 bits (859), Expect = 7.1e-91
Identity = 174/323 (53.87%), Postives = 227/323 (70.28%), Query Frame = 1


Query: 30  RRLRRTKSAPHANSPTEITHTSNVPATGPVPRSGLIFGNLHPSFRRVALVLITYLGIGTL 89
           RR RR ++AP +  P   T   N  A    P++ L  G   PSFR V L+L+ YL +GT+
Sbjct: 25  RRFRRCRTAPSSEPPP--TDKDNSSAADAPPKT-LFTGGGRPSFRLVGLLLVAYLLLGTI 84

Query: 90  CFYLVRHQIQGEKTNRLVDAIYFTIVTMTTVGYGDLVPNSPSTKLLACAFVFTGMALVGL 149
            FYL    + G +T R +DA+YF +VTMTTVGYGDLVP S + KLLACAFVF G+A+VG 
Sbjct: 85  AFYLAMDHMSGTRTTRALDALYFCVVTMTTVGYGDLVPASDAAKLLACAFVFAGVAVVGT 144

Query: 150 ILSNAADYLVEKQEILLFKAFHIDQNGHCDISKEIDTNKARNKCIVVFLLLLLFIISGTA 209
            LS AADYLVEKQE LLF+A H     H  + + ++ NK R K     LLL+  + SGT 
Sbjct: 145 FLSKAADYLVEKQEALLFRALH----SHTMV-RAMEMNKVRYKLYTAGLLLVAAVASGTV 204

Query: 210 FLVTIEKLDFIDAFYCVCSTITTLGYGDQSFSTKWGRVFAIFWILISTITLAQFFLYIAE 269
            L  +E +  +DAFYCVC+T+TTLGYGD+SFS++ GR FA+ WI +ST+ +A FFLY AE
Sbjct: 205 VLWKVEGMRAVDAFYCVCATVTTLGYGDRSFSSEGGRAFAVAWITVSTVVVALFFLYAAE 264

Query: 270 LNTERRQKSLVKWVLSKKVTDIDLEVADIDDDGVVGAAEFVIYKLKEMGKITEDDISLVL 329
           L TERRQ+ L +WVL ++ T++DLE AD+D D  VGAA+FV+YKLKE+GKI+++DIS  L
Sbjct: 265 LYTERRQRELARWVLRRRTTNMDLEAADLDGDHRVGAADFVLYKLKELGKISQEDISEFL 324

Query: 330 NEFENLDVDQSGTLSISDITLAQ 353
           +EF+NLD D SGTLS +D+  AQ
Sbjct: 325 DEFDNLDADHSGTLSPADLAAAQ 339

[Close]

BLAST of Cucsa.279470 vs. Swiss-Prot
Match: KCO3_ORYSJ (Two pore potassium channel c OS=Oryza sativa subsp. japonica GN=TPKC PE=3 SV=1)

HSP 1 Score: 197.6 bits (501), Expect = 2.3e-49
Identity = 115/335 (34.33%), Postives = 189/335 (56.42%), Query Frame = 1


Query: 29  KRRLRRTKSAPHANSPTEITHTSNVPATG------PVPRSGLIFGNLHPSFRRVALVLIT 88
           +  L R+++AP A +P      +   A+G      P PR   I   LH       L L+ 
Sbjct: 113 RTNLHRSRTAP-AMAPLNAAAIAAAAASGDSRNPPPPPRRPAIV--LH-----AFLFLLA 172

Query: 89  YLGIGTLCFYLVRHQIQGEK--TNRLVDAIYFTIVTMTTVGYGDLVPNSPSTKLLACAFV 148
           YL +G   +  +          T+ + DA+YF IVT+ T+GYGD+ P +P+ KL + +FV
Sbjct: 173 YLAMGVTFYAALPGNFTSSAGPTHPVADALYFCIVTLCTIGYGDITPATPAAKLFSISFV 232

Query: 149 FTGMALVGLILSNAADYLVEKQEILLFKAFH----IDQNGH---CDISKEIDTNKARNKC 208
             G   V ++LS    Y+++ QE LL  A      + ++ H    D+ K     + R K 
Sbjct: 233 LIGFGFVDILLSGMVSYVLDLQEHLLITALKNPRSVRKHRHNYIFDLKK--GRMRVRMKV 292

Query: 209 IVVFLLLLLFIISGTAFLVTIEKLDFIDAFYCVCSTITTLGYGDQSFSTKWGRVFAIFWI 268
            +   ++ + +  G A L  +E L ++DA Y    ++TT+GYGD +F T  GR+FA  W+
Sbjct: 293 ALALTVVAICVGVGAAVLKRVENLGWLDAVYLAVMSVTTVGYGDHAFRTLAGRLFASAWL 352

Query: 269 LISTITLAQFFLYIAELNTERRQKSLVKWVLSKKVTDIDLEVADIDDDGVVGAAEFVIYK 328
           L+ST+ +A+ FLY+AE+  ++R +++  WVLS+ +T  +   ADID++G V  +EFV+YK
Sbjct: 353 LVSTLAVARAFLYLAEMRIDKRHRAMANWVLSRDMTVSEFLAADIDNNGYVTKSEFVVYK 412

Query: 329 LKEMGKITEDDISLVLNEFENLDVDQSGTLSISDI 349
           LKEMGKI+E DI ++ ++F+ +D    G +++SD+
Sbjct: 413 LKEMGKISEKDIMMICDQFQRMDSGNCGKITLSDL 437

[Close]

BLAST of Cucsa.279470 vs. Swiss-Prot
Match: KCO5_ARATH (Two-pore potassium channel 5 OS=Arabidopsis thaliana GN=TPK5 PE=1 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 3.9e-49
Identity = 106/286 (37.06%), Postives = 170/286 (59.44%), Query Frame = 1


Query: 74  RRVALVLITYLGIGTLCFYLVRHQIQGEKTNRLVDAIYFTIVTMTTVGYGDLVPNSPSTK 133
           R+   +LI YL +G   +   R    G +T+ +VDA+YF IVTM T+GYGD+ P +P TK
Sbjct: 118 RQAIFLLIVYLTLGVSIYSFNRDHYSGIETHPVVDALYFCIVTMCTIGYGDIAPLTPWTK 177

Query: 134 LLACAFVFTGMALVGLILSNAADYLVEKQEILLF-----KAFHIDQNGHCDISKE--IDT 193
           + A  FV  G   + ++LS   +Y+++ QE ++      +  H   + H   +K+  ID 
Sbjct: 178 IFAVVFVLFGFGFLDILLSGVVNYVLDLQESMILTGIQTRQHHQHHHHHRFSAKDYIIDF 237

Query: 194 NKARN----KCIVVFLLLLLFIISGTAFLVTIEKLDFIDAFYCVCSTITTLGYGDQSFST 253
            K R     K  +   +++L I  G   L  +E+L F+D+ Y    ++TT+GYGD++F T
Sbjct: 238 EKGRMRIRMKVCLALCVVVLCIGVGALVLHFVEELGFVDSVYLSVMSVTTVGYGDRAFKT 297

Query: 254 KWGRVFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWVLSKKVTDIDLEVADIDDDG 313
             GR+FA  W+L+ST+ +A+ FLY+AE   +RR +  VK  L++++T  DL  AD    G
Sbjct: 298 LQGRLFAAVWLLVSTLAVARAFLYLAEARIDRRHRKAVKLALNREITVDDLLKADTYQHG 357

Query: 314 VVGAAEFVIYKLKEMGKITEDDISLVLNEFENLDVDQSGTLSISDI 349
            +  +E+++ KLKEMGKIT+ DI  V+ +FE LD +Q G +++ D+
Sbjct: 358 FISKSEYIVLKLKEMGKITQKDIDQVVIQFEKLDPNQIGKITLPDL 403

[Close]

BLAST of Cucsa.279470 vs. TrEMBL
Match: A0A0A0K3G0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G098760 PE=4 SV=1)

HSP 1 Score: 696.4 bits (1796), Expect = 1.8e-197
Identity = 354/354 (100.00%), Postives = 354/354 (100.00%), Query Frame = 1


Query: 1   MGSQDARQPLLPTSSNTLETRVINIPRSKRRLRRTKSAPHANSPTEITHTSNVPATGPVP 60
           MGSQDARQPLLPTSSNTLETRVINIPRSKRRLRRTKSAPHANSPTEITHTSNVPATGPVP
Sbjct: 1   MGSQDARQPLLPTSSNTLETRVINIPRSKRRLRRTKSAPHANSPTEITHTSNVPATGPVP 60

Query: 61  RSGLIFGNLHPSFRRVALVLITYLGIGTLCFYLVRHQIQGEKTNRLVDAIYFTIVTMTTV 120
           RSGLIFGNLHPSFRRVALVLITYLGIGTLCFYLVRHQIQGEKTNRLVDAIYFTIVTMTTV
Sbjct: 61  RSGLIFGNLHPSFRRVALVLITYLGIGTLCFYLVRHQIQGEKTNRLVDAIYFTIVTMTTV 120

Query: 121 GYGDLVPNSPSTKLLACAFVFTGMALVGLILSNAADYLVEKQEILLFKAFHIDQNGHCDI 180
           GYGDLVPNSPSTKLLACAFVFTGMALVGLILSNAADYLVEKQEILLFKAFHIDQNGHCDI
Sbjct: 121 GYGDLVPNSPSTKLLACAFVFTGMALVGLILSNAADYLVEKQEILLFKAFHIDQNGHCDI 180

Query: 181 SKEIDTNKARNKCIVVFLLLLLFIISGTAFLVTIEKLDFIDAFYCVCSTITTLGYGDQSF 240
           SKEIDTNKARNKCIVVFLLLLLFIISGTAFLVTIEKLDFIDAFYCVCSTITTLGYGDQSF
Sbjct: 181 SKEIDTNKARNKCIVVFLLLLLFIISGTAFLVTIEKLDFIDAFYCVCSTITTLGYGDQSF 240

Query: 241 STKWGRVFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWVLSKKVTDIDLEVADIDD 300
           STKWGRVFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWVLSKKVTDIDLEVADIDD
Sbjct: 241 STKWGRVFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWVLSKKVTDIDLEVADIDD 300

Query: 301 DGVVGAAEFVIYKLKEMGKITEDDISLVLNEFENLDVDQSGTLSISDITLAQLS 355
           DGVVGAAEFVIYKLKEMGKITEDDISLVLNEFENLDVDQSGTLSISDITLAQLS
Sbjct: 301 DGVVGAAEFVIYKLKEMGKITEDDISLVLNEFENLDVDQSGTLSISDITLAQLS 354

[Close]

BLAST of Cucsa.279470 vs. TrEMBL
Match: A0A059BX26_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_F03693 PE=4 SV=1)

HSP 1 Score: 454.1 bits (1167), Expect = 1.5e-124
Identity = 234/355 (65.92%), Postives = 285/355 (80.28%), Query Frame = 1


Query: 1   MGSQDARQPLLPTSSNTLETRVINIPRSKRRLRRTKSAPHANSPTEITHTSNVPATGPVP 60
           M +   +QPLL    N+  T    + R+  RLRR KSAP A +   +   +N+   GP+ 
Sbjct: 1   MDANGVKQPLLSKPFNSPRTDKKELKRN--RLRRCKSAPLAEA---VLQEANL--NGPIQ 60

Query: 61  RSGLIFGNLHPSFRRVALVLITYLGIGTLCFYLVRHQIQGEKTNRLVDAIYFTIVTMTTV 120
            S  I  NLHPSF+RVA++L+ YLGIGT+CFYLVR++I GEKTN L DA+YF IVTMTTV
Sbjct: 61  PSNSILRNLHPSFKRVAIILVFYLGIGTMCFYLVRNEIDGEKTNDLFDAVYFCIVTMTTV 120

Query: 121 GYGDLVPNSPSTKLLACAFVFTGMALVGLILSNAADYLVEKQEILLFKAFHI-DQNGHCD 180
           GYGDLVP S  TKLLACAFVF+GMA+VGLILS AADYLVEKQEILL KA H+ ++ G  +
Sbjct: 121 GYGDLVPGSALTKLLACAFVFSGMAIVGLILSRAADYLVEKQEILLVKALHLRNKVGPTE 180

Query: 181 ISKEIDTNKARNKCIVVFLLLLLFIISGTAFLVTIEKLDFIDAFYCVCSTITTLGYGDQS 240
           + KEI+TN  R KC+  F+LLLL I++GT FL  IE LD +DAFYCVCSTITTLGYGD+S
Sbjct: 181 MLKEIETNGVRYKCVTAFILLLLLIVAGTTFLALIENLDIVDAFYCVCSTITTLGYGDKS 240

Query: 241 FSTKWGRVFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWVLSKKVTDIDLEVADID 300
           FST+ GR+FA+FWIL STI LAQFFLYIAELNTE RQ++LVKWVLS+++T++DLE AD+D
Sbjct: 241 FSTEGGRIFAVFWILTSTICLAQFFLYIAELNTENRQRALVKWVLSRRMTNVDLEAADLD 300

Query: 301 DDGVVGAAEFVIYKLKEMGKITEDDISLVLNEFENLDVDQSGTLSISDITLAQLS 355
           +DGVVGAAEF+IYKLKEMGKI+++DISL+L EF++LDVDQSGTLS SDITLAQ S
Sbjct: 301 NDGVVGAAEFIIYKLKEMGKISQEDISLILEEFDDLDVDQSGTLSGSDITLAQSS 348

[Close]

BLAST of Cucsa.279470 vs. TrEMBL
Match: I3S745_LOTJA (Uncharacterized protein OS=Lotus japonicus PE=2 SV=1)

HSP 1 Score: 453.8 bits (1166), Expect = 2.0e-124
Identity = 235/355 (66.20%), Postives = 283/355 (79.72%), Query Frame = 1


Query: 1   MGSQDARQPLLPTSSNTLETRVINIPRSKRRLRRTKSAPHANSPTEITHTSNVPATGPVP 60
           M + D+++PL+  S +    +   +   KRRL R++SAP  +     T+ + +     +P
Sbjct: 1   MANNDSQEPLISGSMDPSAQKA-RLQLKKRRLIRSRSAPQTDLDPPETNGNKL-----IP 60

Query: 61  RSGLIFGNLHPSFRRVALVLITYLGIGTLCFYLVRHQIQGEKTNRLVDAIYFTIVTMTTV 120
            SG IFG+LHPSFR+VAL L  Y+G+G L FYLVR+QI+G KTNR +DA+YFTIVTMTTV
Sbjct: 61  LSGSIFGSLHPSFRKVALCLAVYVGVGALAFYLVRNQIKGLKTNRFLDALYFTIVTMTTV 120

Query: 121 GYGDLVPNSPSTKLLACAFVFTGMALVGLILSNAADYLVEKQEILLFKAFHIDQN-GHCD 180
           GYGDLVPNS  TKLLACAFVF+GMA+VGLILS AADYLVEKQE LL KA H+ +N G  +
Sbjct: 121 GYGDLVPNSNLTKLLACAFVFSGMAVVGLILSKAADYLVEKQEALLVKAMHMRENFGPSE 180

Query: 181 ISKEIDTNKARNKCIVVFLLLLLFIISGTAFLVTIEKLDFIDAFYCVCSTITTLGYGDQS 240
           I KEI+TNK R K ++V LLLL+ I  GT FLV++EKLDF+DAFYCVCSTITTLGYGD+S
Sbjct: 181 ILKEIETNKTRYKFLLVLLLLLVLITVGTIFLVSVEKLDFVDAFYCVCSTITTLGYGDKS 240

Query: 241 FSTKWGRVFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWVLSKKVTDIDLEVADID 300
           FST+ GRVFA+ WILI TIT+AQFFLY+AELNTE RQK L KWVL +K+T +DLE AD+D
Sbjct: 241 FSTQAGRVFAVIWILIGTITVAQFFLYMAELNTESRQKELAKWVLERKITHLDLEAADLD 300

Query: 301 DDGVVGAAEFVIYKLKEMGKITEDDISLVLNEFENLDVDQSGTLSISDITLAQLS 355
           DDG VGAAEFVIYKLKEMGKI+++DISL L EFE LDVDQSGTLS+SDITLAQ S
Sbjct: 301 DDGTVGAAEFVIYKLKEMGKISQEDISLFLKEFEELDVDQSGTLSVSDITLAQSS 349

[Close]

BLAST of Cucsa.279470 vs. TrEMBL
Match: G7K4P7_MEDTR (Outward rectifying potassium channel protein OS=Medicago truncatula GN=MTR_5g095650 PE=4 SV=1)

HSP 1 Score: 451.4 bits (1160), Expect = 9.9e-124
Identity = 232/356 (65.17%), Postives = 285/356 (80.06%), Query Frame = 1


Query: 1   MGSQDARQPLLPTSSNTLETRVINIPRSKRRLRRTKSAPHAN-SPTEITHTSNVPATGPV 60
           M S D  +PLL  S +T+  +      ++RRL R++SAPH + +P  I    ++P     
Sbjct: 1   MASDDTNEPLLLRSQDTVAQKAKG-RLNQRRLLRSRSAPHTDHAPLVINDKESIPL---- 60

Query: 61  PRSGLIFGNLHPSFRRVALVLITYLGIGTLCFYLVRHQIQGEKTNRLVDAIYFTIVTMTT 120
             S  IFGNLHPSF++VA++L+ YLG+GTL FYLVR+QI+G KT R +DA+YFTIVTMTT
Sbjct: 61  --SETIFGNLHPSFKKVAIILMVYLGVGTLIFYLVRNQIKGMKTERFLDALYFTIVTMTT 120

Query: 121 VGYGDLVPNSPSTKLLACAFVFTGMALVGLILSNAADYLVEKQEILLFKAFHIDQN-GHC 180
           VGYGDLVPNS  TKLLACAFVF+GMAL+GLILS AADYLVEKQE+LL KA H+ Q  G  
Sbjct: 121 VGYGDLVPNSDLTKLLACAFVFSGMALMGLILSKAADYLVEKQEVLLIKAMHMRQKVGPS 180

Query: 181 DISKEIDTNKARNKCIVVFLLLLLFIISGTAFLVTIEKLDFIDAFYCVCSTITTLGYGDQ 240
           +I KE++ NK R K  +VFLLLL+ +I GT FLV +EKL+ IDAFYCVCSTITTLGYGD+
Sbjct: 181 EILKELEINKTRYKFFLVFLLLLILVIVGTIFLVNVEKLEVIDAFYCVCSTITTLGYGDK 240

Query: 241 SFSTKWGRVFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWVLSKKVTDIDLEVADI 300
           SFST+ GR+FA+FWIL  TI LAQFFLY+AELNTE RQK+LV WVL++K+T+ DLE AD+
Sbjct: 241 SFSTQAGRIFAVFWILTGTICLAQFFLYMAELNTESRQKALVNWVLTRKMTNYDLEAADL 300

Query: 301 DDDGVVGAAEFVIYKLKEMGKITEDDISLVLNEFENLDVDQSGTLSISDITLAQLS 355
           D+DG VGAAEFVIYKLKEMGKI+++DI+LV+ EFE LD+DQSGTLS+SDITLAQ S
Sbjct: 301 DEDGTVGAAEFVIYKLKEMGKISQEDITLVMKEFEELDIDQSGTLSVSDITLAQSS 349

[Close]

BLAST of Cucsa.279470 vs. TrEMBL
Match: Q9LLM5_EUCCA (Outward-rectifying potassium channel KCO1 OS=Eucalyptus camaldulensis PE=2 SV=1)

HSP 1 Score: 451.1 bits (1159), Expect = 1.3e-123
Identity = 234/355 (65.92%), Postives = 283/355 (79.72%), Query Frame = 1


Query: 1   MGSQDARQPLLPTSSNTLETRVINIPRSKRRLRRTKSAPHANSPTEITHTSNVPATGPVP 60
           M +   +QPLL    N+ +T    + R+  RLRR KSAP A +   +   +N+   GPV 
Sbjct: 1   MDANGVKQPLLSKPLNSPQTDKKELKRN--RLRRCKSAPLAEA---VPQEANL--NGPVQ 60

Query: 61  RSGLIFGNLHPSFRRVALVLITYLGIGTLCFYLVRHQIQGEKTNRLVDAIYFTIVTMTTV 120
            S  I  NLHPSF+RVA++L+ YLGIGT+CFYLVR++I GEKTN L DA+YF IVTMTTV
Sbjct: 61  PSNSILRNLHPSFKRVAIILVFYLGIGTMCFYLVRNEIDGEKTNDLFDAVYFCIVTMTTV 120

Query: 121 GYGDLVPNSPSTKLLACAFVFTGMALVGLILSNAADYLVEKQEILLFKAFHI-DQNGHCD 180
           GYGDLVP S  TKLLACAFVF+GMA+VGLILS AADYLVEKQEILL KA H+ ++ G  +
Sbjct: 121 GYGDLVPGSALTKLLACAFVFSGMAIVGLILSRAADYLVEKQEILLVKALHLRNKVGPTE 180

Query: 181 ISKEIDTNKARNKCIVVFLLLLLFIISGTAFLVTIEKLDFIDAFYCVCSTITTLGYGDQS 240
           + KEI+TN  R KC+  F+LLLL I++GT FL  IE LD +DAFYCVCSTITTLGYGD+S
Sbjct: 181 MLKEIETNGVRYKCVTAFILLLLLIVAGTTFLALIENLDIVDAFYCVCSTITTLGYGDKS 240

Query: 241 FSTKWGRVFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWVLSKKVTDIDLEVADID 300
           FST+ GR+FA+FWIL STI LAQFFLYIAELNTE RQ++LVKWV S+++T+ DLE AD+D
Sbjct: 241 FSTEGGRIFAVFWILTSTICLAQFFLYIAELNTENRQRALVKWVPSRRMTNFDLEAADLD 300

Query: 301 DDGVVGAAEFVIYKLKEMGKITEDDISLVLNEFENLDVDQSGTLSISDITLAQLS 355
           +DGVVGAAEF+IYKLKEMGKI ++DISL+L EF++LDVDQSGTLS SDITLAQ S
Sbjct: 301 NDGVVGAAEFIIYKLKEMGKINQEDISLILEEFDDLDVDQSGTLSGSDITLAQSS 348

[Close]

BLAST of Cucsa.279470 vs. TAIR10
Match: AT5G55630.1 (AT5G55630.1 Outward rectifying potassium channel protein)

HSP 1 Score: 408.3 bits (1048), Expect = 4.9e-114
Identity = 213/360 (59.17%), Postives = 267/360 (74.17%), Query Frame = 1


Query: 1   MGSQDARQPLLPTSSNTLETRVINI-----PRSKRRLRRTKSAPHANSPTEITHTSNVPA 60
           M S  AR PLLPT       +  N+        KRRLRR++SAP      +  +  +V  
Sbjct: 1   MSSDAARTPLLPTEKIDTMAQDFNLNSRTSSSRKRRLRRSRSAPRG----DCMYNDDVKI 60

Query: 61  TGPVPRSGLI--FGNLHPSFRRVALVLITYLGIGTLCFYLVRHQIQGEKTNRLVDAIYFT 120
             P P    I  F +L+P+ RRV + L  YL IGTLCFYLVR QI G KT+ +VDA+YF 
Sbjct: 61  DEPPPHPSKIPMFSDLNPNLRRVIMFLALYLTIGTLCFYLVRDQISGHKTSGVVDALYFC 120

Query: 121 IVTMTTVGYGDLVPNSPSTKLLACAFVFTGMALVGLILSNAADYLVEKQEILLFKAFHID 180
           IVTMTTVGYGDLVPNS +++LLACAFVF+GM LVG +LS AADYLVEKQE LL +AFH+ 
Sbjct: 121 IVTMTTVGYGDLVPNSSASRLLACAFVFSGMVLVGHLLSRAADYLVEKQEALLVRAFHLR 180

Query: 181 QN-GHCDISKEIDTNKARNKCIVVFLLLLLFIISGTAFLVTIEKLDFIDAFYCVCSTITT 240
           Q+ G  DI KE+ TNK R KC    L+L++  I GT FLV +EK+  I AFYCVCST+TT
Sbjct: 181 QSFGPTDILKELHTNKLRYKCYATCLVLVVLFIVGTIFLVMVEKMPVISAFYCVCSTVTT 240

Query: 241 LGYGDQSFSTKWGRVFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWVLSKKVTDID 300
           LGYGD+SF+++ GR+FA+FWIL S+I LAQFFLY+AELNTE +Q++LVKWVL++++T+ D
Sbjct: 241 LGYGDKSFNSEAGRLFAVFWILTSSICLAQFFLYVAELNTENKQRALVKWVLTRRITNND 300

Query: 301 LEVADIDDDGVVGAAEFVIYKLKEMGKITEDDISLVLNEFENLDVDQSGTLSISDITLAQ 353
           LE AD+D+DGVVGAAEF++YKLKEMGKI E DIS +++EFE LD D+SGTL+ SDI LAQ
Sbjct: 301 LEAADLDEDGVVGAAEFIVYKLKEMGKIDEKDISGIMDEFEQLDYDESGTLTTSDIVLAQ 356

[Close]

BLAST of Cucsa.279470 vs. TAIR10
Match: AT4G01840.1 (AT4G01840.1 Ca2+ activated outward rectifying K+ channel 5)

HSP 1 Score: 196.8 bits (499), Expect = 2.2e-50
Identity = 106/286 (37.06%), Postives = 170/286 (59.44%), Query Frame = 1


Query: 74  RRVALVLITYLGIGTLCFYLVRHQIQGEKTNRLVDAIYFTIVTMTTVGYGDLVPNSPSTK 133
           R+   +LI YL +G   +   R    G +T+ +VDA+YF IVTM T+GYGD+ P +P TK
Sbjct: 118 RQAIFLLIVYLTLGVSIYSFNRDHYSGIETHPVVDALYFCIVTMCTIGYGDIAPLTPWTK 177

Query: 134 LLACAFVFTGMALVGLILSNAADYLVEKQEILLF-----KAFHIDQNGHCDISKE--IDT 193
           + A  FV  G   + ++LS   +Y+++ QE ++      +  H   + H   +K+  ID 
Sbjct: 178 IFAVVFVLFGFGFLDILLSGVVNYVLDLQESMILTGIQTRQHHQHHHHHRFSAKDYIIDF 237

Query: 194 NKARN----KCIVVFLLLLLFIISGTAFLVTIEKLDFIDAFYCVCSTITTLGYGDQSFST 253
            K R     K  +   +++L I  G   L  +E+L F+D+ Y    ++TT+GYGD++F T
Sbjct: 238 EKGRMRIRMKVCLALCVVVLCIGVGALVLHFVEELGFVDSVYLSVMSVTTVGYGDRAFKT 297

Query: 254 KWGRVFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWVLSKKVTDIDLEVADIDDDG 313
             GR+FA  W+L+ST+ +A+ FLY+AE   +RR +  VK  L++++T  DL  AD    G
Sbjct: 298 LQGRLFAAVWLLVSTLAVARAFLYLAEARIDRRHRKAVKLALNREITVDDLLKADTYQHG 357

Query: 314 VVGAAEFVIYKLKEMGKITEDDISLVLNEFENLDVDQSGTLSISDI 349
            +  +E+++ KLKEMGKIT+ DI  V+ +FE LD +Q G +++ D+
Sbjct: 358 FISKSEYIVLKLKEMGKITQKDIDQVVIQFEKLDPNQIGKITLPDL 403

[Close]

BLAST of Cucsa.279470 vs. TAIR10
Match: AT4G18160.1 (AT4G18160.1 Ca2+ activated outward rectifying K+ channel 6)

HSP 1 Score: 192.6 bits (488), Expect = 4.2e-49
Identity = 110/321 (34.27%), Postives = 176/321 (54.83%), Query Frame = 1


Query: 32  LRRTKSAPHANSPTEITHTSNVPATGPVPRSGLIFGNLHPSFRRVALVLITYLGIGTLCF 91
           L R+K+AP      ++ H           RS +         R+   +L+ YL +G L +
Sbjct: 118 LHRSKTAPAMAVINDLHHPIRQKDPTETSRSVV---------RQAFALLVVYLSLGVLIY 177

Query: 92  YLVRHQIQGEKTNRLVDAIYFTIVTMTTVGYGDLVPNSPSTKLLACAFVFTGMALVGLIL 151
           +L R      +T+ +VD +YF IVTM T+GYGD+ PNS  TKL +  FV  G   + ++L
Sbjct: 178 WLNRDHYVVNQTHPVVDGLYFCIVTMCTIGYGDITPNSVVTKLFSIMFVLVGFGFIDILL 237

Query: 152 SNAADYLVEKQEILLFKAFHIDQNGHCDISKEIDTNKARN----KCIVVFLLLLLFIISG 211
           S    Y+++ QE  +  +           S  ID  K R     K  +   +++L I  G
Sbjct: 238 SGMVSYVLDLQESYMLDSAKRRDEPEKRRSYIIDVKKGRMRIRLKVALALGVVVLCIAVG 297

Query: 212 TAFLVTIEKLDFIDAFYCVCSTITTLGYGDQSFSTKWGRVFAIFWILISTITLAQFFLYI 271
              +  IE++ ++D+FY    ++TT+GYGD++F T  GR+FA  W+L+ST+ +A+ FLY+
Sbjct: 298 VGIMHFIEEIGWLDSFYLSVMSVTTVGYGDRAFKTLPGRLFAAIWLLVSTLAVARAFLYL 357

Query: 272 AELNTERRQKSLVKWVLSKKVTDIDLEVADIDDDGVVGAAEFVIYKLKEMGKITEDDISL 331
           AE   ++R +   K VL + ++      ADID++G V  AE+VIYKLKEM KIT+ DI  
Sbjct: 358 AEARVDKRNRERAKKVLCETMSVSQFFAADIDNNGCVSKAEYVIYKLKEMEKITDKDILP 417

Query: 332 VLNEFENLDVDQSGTLSISDI 349
           +  +F+ LD   +G +++ D+
Sbjct: 418 ISKQFDKLDRCSNGKITLLDL 429

[Close]

BLAST of Cucsa.279470 vs. TAIR10
Match: AT5G46370.1 (AT5G46370.1 Ca2+ activated outward rectifying K+ channel 2)

HSP 1 Score: 184.9 bits (468), Expect = 8.7e-47
Identity = 116/323 (35.91%), Postives = 181/323 (56.04%), Query Frame = 1


Query: 34  RTKSAPHANSPTEITHTSNVPATGPVPRSGLIFGNLHPSFRRVALVLITYLGIGTLCFYL 93
           R+K+AP   +   I+H  N P T     S  I        + VAL L+ YL +G L ++L
Sbjct: 115 RSKTAPAMAAINNISHP-NDPKTDQQSDSKTIVN------QAVAL-LVVYLSLGVLIYWL 174

Query: 94  VRHQIQGEKTNRLVDAIYFTIVTMTTVGYGDLVPNSPSTKLLACAFVFTGMALVGLILSN 153
            R     ++T+ +VDA+YF IVTM T+GYGD+ P+S  TKL +  FV  G   + ++LS 
Sbjct: 175 NRDSYNVKQTHPVVDALYFCIVTMCTIGYGDITPDSVVTKLFSIFFVLVGFGFMDILLSG 234

Query: 154 AADYLVEKQEILLFKAFHIDQNGHCDISKE----IDTNKARNKCIVVFLLLLLFIISGTA 213
              Y+++ QE  + +    +     D  K     ID  K R +  +   L L  ++    
Sbjct: 235 MVTYVLDLQENYMLETARNESLNLNDRDKVRSYIIDVKKGRMRIRLKVGLALGVVVLCLG 294

Query: 214 FLVTI----EKLDFIDAFYCVCSTITTLGYGDQSFSTKWGRVFAIFWILISTITLAQFFL 273
           F V I    EK+ ++D+FY    ++TT+GYGD++F+T  GR+ A  W+L+ST+ +A+  L
Sbjct: 295 FGVLIMHFVEKIGWLDSFYFSVMSVTTVGYGDRAFNTLAGRLLAAMWLLVSTLAVARAIL 354

Query: 274 YIAELNTERRQKSLVKWVLSKKVTDIDLEVADIDDDGVVGAAEFVIYKLKEMGKITEDDI 333
           ++AE   ++R +   K VL + ++      ADID +G V  AEFVIYKLK+M KITE DI
Sbjct: 355 FLAESRVDKRNRERAKKVLGESMSISQFLDADIDCNGCVSKAEFVIYKLKKMDKITEKDI 414

Query: 334 SLVLNEFENLDVDQSGTLSISDI 349
           + +  +F+ LD   SG +++ D+
Sbjct: 415 NPIGFQFDKLDRTNSGRITLLDL 429

[Close]

BLAST of Cucsa.279470 vs. TAIR10
Match: AT1G02510.1 (AT1G02510.1 Outward rectifying potassium channel protein)

HSP 1 Score: 167.9 bits (424), Expect = 1.1e-41
Identity = 95/267 (35.58%), Postives = 152/267 (56.93%), Query Frame = 1


Query: 78  LVLITYLGIGTLCFYLVRHQIQGEKTNRLVDAIYFTIVTMTTVGYGDLVPNSPSTKLLAC 137
           ++L+ YL  G   +   R Q  G +TN  VDA YF+IVT +TVGYGD+VP++ +TK+L  
Sbjct: 40  ILLLVYLTFGVCTYSFFRDQFSGTETNLFVDAFYFSIVTFSTVGYGDIVPSTSTTKILTI 99

Query: 138 AFVFTGMALVGLILSNAADYLVEKQEILLFKAFHIDQNGHC-DISKEIDTNKARNKCIVV 197
             V TG+  +  +L+    +++  QE  +    +  +N    D   E    + + K  + 
Sbjct: 100 VLVSTGVVFLDYLLNRVVSHVLSLQENAILDRINKTRNRAIRDHIAEDGKIRLKWKLCLA 159

Query: 198 FLLLLLFIISGTAFLVTIEKLDFIDAFYCVCSTITTLGYGDQSFSTKWGRVFAIFWILIS 257
           F  + L + SG  FL   E+LD++D+ Y    ++TT+GYGD++F T  GR FA+FW+L+S
Sbjct: 160 FCAVGLCVGSGALFLHVFERLDWLDSVYLSVISVTTVGYGDKTFKTVEGRGFAVFWLLLS 219

Query: 258 TITLAQFFLYIAELNTERRQKSLVKWVLSKKVTDIDLEVADIDDDGVVGAAEFVIYKLKE 317
           TI +A  FLY+AE+  +R              T + L  ++         +EF+++KL+E
Sbjct: 220 TIAMATLFLYLAEMRIDR-------------TTVMKLPPSE---------SEFIVFKLRE 279

Query: 318 MGKITEDDISLVLNEFENL-DVDQSGT 343
            G+I+EDDI  ++ EFENL +V  SG+
Sbjct: 280 SGRISEDDIKQIVREFENLEEVPSSGS 284

[Close]

BLAST of Cucsa.279470 vs. NCBI nr
Match: gi|449466149|ref|XP_004150789.1| (PREDICTED: two-pore potassium channel 1 [Cucumis sativus])

HSP 1 Score: 696.4 bits (1796), Expect = 2.5e-197
Identity = 354/354 (100.00%), Postives = 354/354 (100.00%), Query Frame = 1


Query: 1   MGSQDARQPLLPTSSNTLETRVINIPRSKRRLRRTKSAPHANSPTEITHTSNVPATGPVP 60
           MGSQDARQPLLPTSSNTLETRVINIPRSKRRLRRTKSAPHANSPTEITHTSNVPATGPVP
Sbjct: 1   MGSQDARQPLLPTSSNTLETRVINIPRSKRRLRRTKSAPHANSPTEITHTSNVPATGPVP 60

Query: 61  RSGLIFGNLHPSFRRVALVLITYLGIGTLCFYLVRHQIQGEKTNRLVDAIYFTIVTMTTV 120
           RSGLIFGNLHPSFRRVALVLITYLGIGTLCFYLVRHQIQGEKTNRLVDAIYFTIVTMTTV
Sbjct: 61  RSGLIFGNLHPSFRRVALVLITYLGIGTLCFYLVRHQIQGEKTNRLVDAIYFTIVTMTTV 120

Query: 121 GYGDLVPNSPSTKLLACAFVFTGMALVGLILSNAADYLVEKQEILLFKAFHIDQNGHCDI 180
           GYGDLVPNSPSTKLLACAFVFTGMALVGLILSNAADYLVEKQEILLFKAFHIDQNGHCDI
Sbjct: 121 GYGDLVPNSPSTKLLACAFVFTGMALVGLILSNAADYLVEKQEILLFKAFHIDQNGHCDI 180

Query: 181 SKEIDTNKARNKCIVVFLLLLLFIISGTAFLVTIEKLDFIDAFYCVCSTITTLGYGDQSF 240
           SKEIDTNKARNKCIVVFLLLLLFIISGTAFLVTIEKLDFIDAFYCVCSTITTLGYGDQSF
Sbjct: 181 SKEIDTNKARNKCIVVFLLLLLFIISGTAFLVTIEKLDFIDAFYCVCSTITTLGYGDQSF 240

Query: 241 STKWGRVFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWVLSKKVTDIDLEVADIDD 300
           STKWGRVFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWVLSKKVTDIDLEVADIDD
Sbjct: 241 STKWGRVFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWVLSKKVTDIDLEVADIDD 300

Query: 301 DGVVGAAEFVIYKLKEMGKITEDDISLVLNEFENLDVDQSGTLSISDITLAQLS 355
           DGVVGAAEFVIYKLKEMGKITEDDISLVLNEFENLDVDQSGTLSISDITLAQLS
Sbjct: 301 DGVVGAAEFVIYKLKEMGKITEDDISLVLNEFENLDVDQSGTLSISDITLAQLS 354

[Close]

BLAST of Cucsa.279470 vs. NCBI nr
Match: gi|659126060|ref|XP_008462994.1| (PREDICTED: two-pore potassium channel 1 isoform X1 [Cucumis melo])

HSP 1 Score: 665.2 bits (1715), Expect = 6.2e-188
Identity = 337/354 (95.20%), Postives = 348/354 (98.31%), Query Frame = 1


Query: 1   MGSQDARQPLLPTSSNTLETRVINIPRSKRRLRRTKSAPHANSPTEITHTSNVPATGPVP 60
           MGS+DARQPLLPTSSNTLETRVI+IPRSKRRLRRTKSAPHANSPTEIT TSNVPATGPVP
Sbjct: 1   MGSKDARQPLLPTSSNTLETRVIDIPRSKRRLRRTKSAPHANSPTEITRTSNVPATGPVP 60

Query: 61  RSGLIFGNLHPSFRRVALVLITYLGIGTLCFYLVRHQIQGEKTNRLVDAIYFTIVTMTTV 120
           RSGLIFGNLHPSFRRVALVLI YLGIGTLCFYLVR+QI+GEK+N +VDAIYFTIVTMTTV
Sbjct: 61  RSGLIFGNLHPSFRRVALVLIIYLGIGTLCFYLVRYQIKGEKSNGIVDAIYFTIVTMTTV 120

Query: 121 GYGDLVPNSPSTKLLACAFVFTGMALVGLILSNAADYLVEKQEILLFKAFHIDQNGHCDI 180
           GYGDLVPNSPSTKLLACAFVF+GMALVGLILSNAADYLVEKQEILLFKAFHI QNGHCDI
Sbjct: 121 GYGDLVPNSPSTKLLACAFVFSGMALVGLILSNAADYLVEKQEILLFKAFHIHQNGHCDI 180

Query: 181 SKEIDTNKARNKCIVVFLLLLLFIISGTAFLVTIEKLDFIDAFYCVCSTITTLGYGDQSF 240
           SKEIDTNKARNKC+VVFLLLLLFIISGTAFLV IEKLDFIDAFYCVCSTITTLGYGDQSF
Sbjct: 181 SKEIDTNKARNKCVVVFLLLLLFIISGTAFLVIIEKLDFIDAFYCVCSTITTLGYGDQSF 240

Query: 241 STKWGRVFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWVLSKKVTDIDLEVADIDD 300
           STKWGR+FAIFWILISTITLAQFFLYIAELNTERRQKSLVKWVLS+KVTDIDLEVADIDD
Sbjct: 241 STKWGRIFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWVLSEKVTDIDLEVADIDD 300

Query: 301 DGVVGAAEFVIYKLKEMGKITEDDISLVLNEFENLDVDQSGTLSISDITLAQLS 355
           DGVVGAAEFVIYKLKEMGKITEDDIS VL+EFENLDVDQSGTLSISDITLAQLS
Sbjct: 301 DGVVGAAEFVIYKLKEMGKITEDDISPVLDEFENLDVDQSGTLSISDITLAQLS 354

[Close]

BLAST of Cucsa.279470 vs. NCBI nr
Match: gi|702379619|ref|XP_010063273.1| (PREDICTED: two-pore potassium channel 1 isoform X3 [Eucalyptus grandis])

HSP 1 Score: 454.1 bits (1167), Expect = 2.2e-124
Identity = 234/355 (65.92%), Postives = 285/355 (80.28%), Query Frame = 1


Query: 1   MGSQDARQPLLPTSSNTLETRVINIPRSKRRLRRTKSAPHANSPTEITHTSNVPATGPVP 60
           M +   +QPLL    N+  T    + R+  RLRR KSAP A +   +   +N+   GP+ 
Sbjct: 1   MDANGVKQPLLSKPFNSPRTDKKELKRN--RLRRCKSAPLAEA---VLQEANL--NGPIQ 60

Query: 61  RSGLIFGNLHPSFRRVALVLITYLGIGTLCFYLVRHQIQGEKTNRLVDAIYFTIVTMTTV 120
            S  I  NLHPSF+RVA++L+ YLGIGT+CFYLVR++I GEKTN L DA+YF IVTMTTV
Sbjct: 61  PSNSILRNLHPSFKRVAIILVFYLGIGTMCFYLVRNEIDGEKTNDLFDAVYFCIVTMTTV 120

Query: 121 GYGDLVPNSPSTKLLACAFVFTGMALVGLILSNAADYLVEKQEILLFKAFHI-DQNGHCD 180
           GYGDLVP S  TKLLACAFVF+GMA+VGLILS AADYLVEKQEILL KA H+ ++ G  +
Sbjct: 121 GYGDLVPGSALTKLLACAFVFSGMAIVGLILSRAADYLVEKQEILLVKALHLRNKVGPTE 180

Query: 181 ISKEIDTNKARNKCIVVFLLLLLFIISGTAFLVTIEKLDFIDAFYCVCSTITTLGYGDQS 240
           + KEI+TN  R KC+  F+LLLL I++GT FL  IE LD +DAFYCVCSTITTLGYGD+S
Sbjct: 181 MLKEIETNGVRYKCVTAFILLLLLIVAGTTFLALIENLDIVDAFYCVCSTITTLGYGDKS 240

Query: 241 FSTKWGRVFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWVLSKKVTDIDLEVADID 300
           FST+ GR+FA+FWIL STI LAQFFLYIAELNTE RQ++LVKWVLS+++T++DLE AD+D
Sbjct: 241 FSTEGGRIFAVFWILTSTICLAQFFLYIAELNTENRQRALVKWVLSRRMTNVDLEAADLD 300

Query: 301 DDGVVGAAEFVIYKLKEMGKITEDDISLVLNEFENLDVDQSGTLSISDITLAQLS 355
           +DGVVGAAEF+IYKLKEMGKI+++DISL+L EF++LDVDQSGTLS SDITLAQ S
Sbjct: 301 NDGVVGAAEFIIYKLKEMGKISQEDISLILEEFDDLDVDQSGTLSGSDITLAQSS 348

[Close]

BLAST of Cucsa.279470 vs. NCBI nr
Match: gi|702379614|ref|XP_010063272.1| (PREDICTED: two-pore potassium channel 1 isoform X2 [Eucalyptus grandis])

HSP 1 Score: 454.1 bits (1167), Expect = 2.2e-124
Identity = 234/355 (65.92%), Postives = 285/355 (80.28%), Query Frame = 1


Query: 1   MGSQDARQPLLPTSSNTLETRVINIPRSKRRLRRTKSAPHANSPTEITHTSNVPATGPVP 60
           M +   +QPLL    N+  T    + R+  RLRR KSAP A +   +   +N+   GP+ 
Sbjct: 11  MDANGVKQPLLSKPFNSPRTDKKELKRN--RLRRCKSAPLAEA---VLQEANL--NGPIQ 70

Query: 61  RSGLIFGNLHPSFRRVALVLITYLGIGTLCFYLVRHQIQGEKTNRLVDAIYFTIVTMTTV 120
            S  I  NLHPSF+RVA++L+ YLGIGT+CFYLVR++I GEKTN L DA+YF IVTMTTV
Sbjct: 71  PSNSILRNLHPSFKRVAIILVFYLGIGTMCFYLVRNEIDGEKTNDLFDAVYFCIVTMTTV 130

Query: 121 GYGDLVPNSPSTKLLACAFVFTGMALVGLILSNAADYLVEKQEILLFKAFHI-DQNGHCD 180
           GYGDLVP S  TKLLACAFVF+GMA+VGLILS AADYLVEKQEILL KA H+ ++ G  +
Sbjct: 131 GYGDLVPGSALTKLLACAFVFSGMAIVGLILSRAADYLVEKQEILLVKALHLRNKVGPTE 190

Query: 181 ISKEIDTNKARNKCIVVFLLLLLFIISGTAFLVTIEKLDFIDAFYCVCSTITTLGYGDQS 240
           + KEI+TN  R KC+  F+LLLL I++GT FL  IE LD +DAFYCVCSTITTLGYGD+S
Sbjct: 191 MLKEIETNGVRYKCVTAFILLLLLIVAGTTFLALIENLDIVDAFYCVCSTITTLGYGDKS 250

Query: 241 FSTKWGRVFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWVLSKKVTDIDLEVADID 300
           FST+ GR+FA+FWIL STI LAQFFLYIAELNTE RQ++LVKWVLS+++T++DLE AD+D
Sbjct: 251 FSTEGGRIFAVFWILTSTICLAQFFLYIAELNTENRQRALVKWVLSRRMTNVDLEAADLD 310

Query: 301 DDGVVGAAEFVIYKLKEMGKITEDDISLVLNEFENLDVDQSGTLSISDITLAQLS 355
           +DGVVGAAEF+IYKLKEMGKI+++DISL+L EF++LDVDQSGTLS SDITLAQ S
Sbjct: 311 NDGVVGAAEFIIYKLKEMGKISQEDISLILEEFDDLDVDQSGTLSGSDITLAQSS 358

[Close]

BLAST of Cucsa.279470 vs. NCBI nr
Match: gi|702379611|ref|XP_010063270.1| (PREDICTED: two-pore potassium channel 1 isoform X1 [Eucalyptus grandis])

HSP 1 Score: 454.1 bits (1167), Expect = 2.2e-124
Identity = 234/355 (65.92%), Postives = 285/355 (80.28%), Query Frame = 1


Query: 1   MGSQDARQPLLPTSSNTLETRVINIPRSKRRLRRTKSAPHANSPTEITHTSNVPATGPVP 60
           M +   +QPLL    N+  T    + R+  RLRR KSAP A +   +   +N+   GP+ 
Sbjct: 95  MDANGVKQPLLSKPFNSPRTDKKELKRN--RLRRCKSAPLAEA---VLQEANL--NGPIQ 154

Query: 61  RSGLIFGNLHPSFRRVALVLITYLGIGTLCFYLVRHQIQGEKTNRLVDAIYFTIVTMTTV 120
            S  I  NLHPSF+RVA++L+ YLGIGT+CFYLVR++I GEKTN L DA+YF IVTMTTV
Sbjct: 155 PSNSILRNLHPSFKRVAIILVFYLGIGTMCFYLVRNEIDGEKTNDLFDAVYFCIVTMTTV 214

Query: 121 GYGDLVPNSPSTKLLACAFVFTGMALVGLILSNAADYLVEKQEILLFKAFHI-DQNGHCD 180
           GYGDLVP S  TKLLACAFVF+GMA+VGLILS AADYLVEKQEILL KA H+ ++ G  +
Sbjct: 215 GYGDLVPGSALTKLLACAFVFSGMAIVGLILSRAADYLVEKQEILLVKALHLRNKVGPTE 274

Query: 181 ISKEIDTNKARNKCIVVFLLLLLFIISGTAFLVTIEKLDFIDAFYCVCSTITTLGYGDQS 240
           + KEI+TN  R KC+  F+LLLL I++GT FL  IE LD +DAFYCVCSTITTLGYGD+S
Sbjct: 275 MLKEIETNGVRYKCVTAFILLLLLIVAGTTFLALIENLDIVDAFYCVCSTITTLGYGDKS 334

Query: 241 FSTKWGRVFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWVLSKKVTDIDLEVADID 300
           FST+ GR+FA+FWIL STI LAQFFLYIAELNTE RQ++LVKWVLS+++T++DLE AD+D
Sbjct: 335 FSTEGGRIFAVFWILTSTICLAQFFLYIAELNTENRQRALVKWVLSRRMTNVDLEAADLD 394

Query: 301 DDGVVGAAEFVIYKLKEMGKITEDDISLVLNEFENLDVDQSGTLSISDITLAQLS 355
           +DGVVGAAEF+IYKLKEMGKI+++DISL+L EF++LDVDQSGTLS SDITLAQ S
Sbjct: 395 NDGVVGAAEFIIYKLKEMGKISQEDISLILEEFDDLDVDQSGTLSGSDITLAQSS 442

The following BLAST results are available for this feature:

BLAST of Cucsa.279470 vs. Swiss-Prot


Match Name	E-value	Identity	Description
KCO1_ARATH	8.6e-113	59.17	Two-pore potassium channel 1 OS=Arabidopsis thaliana GN=TPK1 PE=1 SV=2	[more]
KCO1_ORYSJ	1.2e-101	54.67	Two pore potassium channel a OS=Oryza sativa subsp. japonica GN=TPKA PE=1 SV=1	[more]
KCO2_ORYSJ	7.1e-91	53.87	Two pore potassium channel b OS=Oryza sativa subsp. japonica GN=TPKB PE=1 SV=1	[more]
KCO3_ORYSJ	2.3e-49	34.33	Two pore potassium channel c OS=Oryza sativa subsp. japonica GN=TPKC PE=3 SV=1	[more]
KCO5_ARATH	3.9e-49	37.06	Two-pore potassium channel 5 OS=Arabidopsis thaliana GN=TPK5 PE=1 SV=1	[more]

BLAST of Cucsa.279470 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K3G0_CUCSA	1.8e-197	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G098760 PE=4 SV=1	[more]
A0A059BX26_EUCGR	1.5e-124	65.92	Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_F03693 PE=4 SV=1	[more]
I3S745_LOTJA	2.0e-124	66.20	Uncharacterized protein OS=Lotus japonicus PE=2 SV=1	[more]
G7K4P7_MEDTR	9.9e-124	65.17	Outward rectifying potassium channel protein OS=Medicago truncatula GN=MTR_5g095...	[more]
Q9LLM5_EUCCA	1.3e-123	65.92	Outward-rectifying potassium channel KCO1 OS=Eucalyptus camaldulensis PE=2 SV=1	[more]

BLAST of Cucsa.279470 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G55630.1	4.9e-114	59.17	Outward rectifying potassium channel protein	[more]
AT4G01840.1	2.2e-50	37.06	Ca2+ activated outward rectifying K+ channel 5	[more]
AT4G18160.1	4.2e-49	34.27	Ca2+ activated outward rectifying K+ channel 6	[more]
AT5G46370.1	8.7e-47	35.91	Ca2+ activated outward rectifying K+ channel 2	[more]
AT1G02510.1	1.1e-41	35.58	Outward rectifying potassium channel protein	[more]

BLAST of Cucsa.279470 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449466149\|ref\|XP_004150789.1\|	2.5e-197	100.00	PREDICTED: two-pore potassium channel 1 [Cucumis sativus]	[more]
gi\|659126060\|ref\|XP_008462994.1\|	6.2e-188	95.20	PREDICTED: two-pore potassium channel 1 isoform X1 [Cucumis melo]	[more]
gi\|702379619\|ref\|XP_010063273.1\|	2.2e-124	65.92	PREDICTED: two-pore potassium channel 1 isoform X3 [Eucalyptus grandis]	[more]
gi\|702379614\|ref\|XP_010063272.1\|	2.2e-124	65.92	PREDICTED: two-pore potassium channel 1 isoform X2 [Eucalyptus grandis]	[more]
gi\|702379611\|ref\|XP_010063270.1\|	2.2e-124	65.92	PREDICTED: two-pore potassium channel 1 isoform X1 [Eucalyptus grandis]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR003280	2pore_dom_K_chnl
IPR011992	EF-hand-dom_pair
IPR013099	K_chnl_dom
IPR018247	EF_Hand_1_Ca_BS

Vocabulary: **Molecular Function**
Term	Definition
GO:0005249	voltage-gated potassium channel activity
GO:0005267	potassium channel activity

Vocabulary: **Cellular Component**
Term	Definition
GO:0005774	vacuolar membrane
GO:0016020	membrane

Vocabulary: **Biological Process**
Term	Definition
GO:0030007	cellular potassium ion homeostasis
GO:0071805	potassium ion transmembrane transport

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006816	calcium ion transport
biological_process	GO:0030007	cellular potassium ion homeostasis
biological_process	GO:0007030	Golgi organization
biological_process	GO:0000271	polysaccharide biosynthetic process
biological_process	GO:0071805	potassium ion transmembrane transport
biological_process	GO:0051260	protein homooligomerization
biological_process	GO:0010029	regulation of seed germination
biological_process	GO:0010119	regulation of stomatal movement
biological_process	GO:0009651	response to salt stress
cellular_component	GO:0005774	vacuolar membrane
cellular_component	GO:0008076	voltage-gated potassium channel complex
cellular_component	GO:0016020	membrane
molecular_function	GO:0005249	voltage-gated potassium channel activity
molecular_function	GO:0005267	potassium channel activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsa.279470.1	Cucsa.279470.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003280	Two pore domain potassium channel	PRINTS	PR01333	2POREKCHANEL	coord: 115..143 score: 8.3E-6coord: 231..240 score: 8.
IPR011992	EF-hand domain pair	GENE3D	G3DSA:1.10.238.10		coord: 270..348 score: 1.
IPR011992	EF-hand domain pair	unknown	SSF47473	EF-hand	coord: 281..349 score: 9.0
IPR013099	Potassium channel domain	PFAM	PF07885	Ion_trans_2	coord: 199..270 score: 5.2E-15coord: 79..159 score: 1.2
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 336..348 score: -coord: 297..309 scor
None	No IPR available	GENE3D	G3DSA:1.10.287.70		coord: 72..162 score: 1.5E-23coord: 190..269 score: 3.3
None	No IPR available	PANTHER	PTHR11003	POTASSIUM CHANNEL, SUBFAMILY K	coord: 64..352 score: 5.2E
None	No IPR available	unknown	SSF81324	Voltage-gated potassium channels	coord: 70..168 score: 1.7E-25coord: 186..291 score: 6.15

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cucsa.279470	Cla003303	Watermelon (97103) v1	cgywmB510
Cucsa.279470	MELO3C024926	Melon (DHL92) v3.5.1	cgymeB466
Cucsa.279470	ClCG09G021230	Watermelon (Charleston Gray)	cgywcgB482
Cucsa.279470	MELO3C024926.2	Melon (DHL92) v3.6.1	cgymedB462
Cucsa.279470	Bhi09G001469	Wax gourd	cgywgoB563

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:

None