Cucsa.275630 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.275630 vs. Swiss-Prot
Match: TMVRN_NICGU (TMV resistance protein N OS=Nicotiana glutinosa GN=N PE=1 SV=1)

HSP 1 Score: 502.3 bits (1292), Expect = 1.2e-140
Identity = 311/803 (38.73%), Postives = 467/803 (58.16%), Query Frame = 1

Query: 4   SSCSSNLKWSYDVFLSFRGEDTRNNFTSHLDRALREKGVNFFIDDK-LERGGQISESLLK 63
           +S SS+ +WSYDVFLSFRGEDTR  FTSHL   L +KG+  F DDK LE G  I   L K
Sbjct: 2   ASSSSSSRWSYDVFLSFRGEDTRKTFTSHLYEVLNDKGIKTFQDDKRLEYGATIPGELCK 61

Query: 64  SIDGSKISIIIFSKNYASSTWCLDELVKIVQCMKSMGHIVFPVFYKVDPSEVRKQTGGFG 123
           +I+ S+ +I++FS+NYA+S WCL+ELVKI++C       V P+FY VDPS VR Q   F 
Sbjct: 62  AIEESQFAIVVFSENYATSRWCLNELVKIMECKTRFKQTVIPIFYDVDPSHVRNQKESFA 121

Query: 124 EALAKHEAN-ELMTNKVQPWKEALTTAASLSGWDLATRKNEADLIHDLVKEVLSILNQTQ 183
           +A  +HE   +     +Q W+ AL  AA+L G      K +AD I  +V ++ S L +  
Sbjct: 122 KAFEEHETKYKDDVEGIQRWRIALNEAANLKGSCDNRDKTDADCIRQIVDQISSKLCKIS 181

Query: 184 LLHVAKHPVGIDSQLRAVEELASHDVPDGVNMVGIHGMGGIGKTTLAKALYNKI------ 243
           L ++ ++ VGID+ L  +E L    + +GV ++GI GMGG+GKTT+A+A+++ +      
Sbjct: 182 LSYL-QNIVGIDTHLEKIESLLEIGI-NGVRIMGIWGMGGVGKTTIARAIFDTLLGRMDS 241

Query: 244 AYQFEACCFLSNVRETLEQFKDLVQLQEKLLSEILKDNAWKVGNVHKGKNIIRDRLCSKK 303
           +YQF+  CFL +++E     + +  LQ  LLSE+L++ A    N   GK+ +  RL SKK
Sbjct: 242 SYQFDGACFLKDIKENK---RGMHSLQNALLSELLREKA-NYNNEEDGKHQMASRLRSKK 301

Query: 304 VLIILDDVD-KDEQLDALVGERDWFGRGSKIIATTRDRHLLENHSFDIVYPIQLLDPKKS 363
           VLI+LDD+D KD  L+ L G+ DWFG GS+II TTRD+HL+E +  DI+Y +  L   +S
Sbjct: 302 VLIVLDDIDNKDHYLEYLAGDLDWFGNGSRIIITTRDKHLIEKN--DIIYEVTALPDHES 361

Query: 364 LELFSLHAFKQNHPSSNYVDLSKFAVSYCKGLPLALVILGSLLHKRERKIWKSKLHELEN 423
           ++LF  HAF +  P+ N+  LS   V+Y KGLPLAL + GSLLH      WKS +  ++N
Sbjct: 362 IQLFKQHAFGKEVPNENFEKLSLEVVNYAKGLPLALKVWGSLLHNLRLTEWKSAIEHMKN 421

Query: 424 SLEPSVEAVFQIGFKELHERVKEIFLDISCFFVGEDINYSKDVLKACDLNPDYGIIILMD 483
           +    +    +I +  L  + +E+FLDI+CF  GE+ +Y   +L++C +  +YG+ IL+D
Sbjct: 422 NSYSGIIDKLKISYDGLEPKQQEMFLDIACFLRGEEKDYILQILESCHIGAEYGLRILID 481

Query: 484 LSLVTV-EDGKIQMHDLIQQMGQTIVRHESFEPAKRSRLWEAEGAIKILKEKSVSDFRQC 543
            SLV + E  ++QMHDLIQ MG+ IV  +  +P +RSRLW A+   +++   + +   + 
Sbjct: 482 KSLVFISEYNQVQMHDLIQDMGKYIVNFQK-DPGERSRLWLAKEVEEVMSNNTGTMAMEA 541

Query: 544 FYLLIAKD---IYSEAFRNMKNLRLLILQRVAYFPRNIFEYLPNSLKWIEWSTFYVNQSS 603
            ++          ++A +NMK LR+  + R +       +YLPN+L+    + +      
Sbjct: 542 IWVSSYSSTLRFSNQAVKNMKRLRVFNMGRSS--THYAIDYLPNNLRCFVCTNY------ 601

Query: 604 SISFSETPNFFATLNLEKLYLRGCTSLKGMRPSFRKFPSHLKFKSLKVLNLRDCLNLEEI 663
              +   P+ F    L  L LR   SL+ +    +  P      SL+ ++L     L   
Sbjct: 602 --PWESFPSTFELKMLVHLQLRH-NSLRHLWTETKHLP------SLRRIDLSWSKRLTRT 661

Query: 664 TDFSMASNLEILDLNTCFSLRIIHESIGSLDKLITLQLDLCHNLEKLPSSLKLKSLDSLS 723
            DF+   NLE ++L  C +L  +H S+G   K+I L L+ C +L++ P  + ++SL+ L 
Sbjct: 662 PDFTGMPNLEYVNLYQCSNLEEVHHSLGCCSKVIGLYLNDCKSLKRFP-CVNVESLEYLG 721

Query: 724 FTNCYKLEQLPEFDENMKSLRVMNLNGTAIRVLPSSI-GYLIGLENLNLNDCANLTALPN 783
             +C  LE+LPE    MK    +++ G+ IR LPSSI  Y   +  L L +  NL ALP+
Sbjct: 722 LRSCDSLEKLPEIYGRMKPEIQIHMQGSGIRELPSSIFQYKTHVTKLLLWNMKNLVALPS 777

Query: 784 EIHWLKSLEELHLRGCSKLDMFP 793
            I  LKSL  L + GCSKL+  P
Sbjct: 782 SICRLKSLVSLSVSGCSKLESLP 777

HSP 2 Score: 83.2 bits (204), Expect = 1.7e-14
Identity = 103/378 (27.25%), Postives = 169/378 (44.71%), Query Frame = 1

Query: 545 EAFRNMKNLRLLILQRVAYFPRNIFEYLPNSLKWIEWSTFYVNQSSSISFSETPNFFATL 604
           E+F +   L++L+  ++ +   N   +L    K +  S   ++ S S   + TP+F    
Sbjct: 587 ESFPSTFELKMLVHLQLRH---NSLRHLWTETKHLP-SLRRIDLSWSKRLTRTPDFTGMP 646

Query: 605 NLEKLYLRGCTSLKGMR-----------------PSFRKFPSHLKFKSLKVLNLRDCLNL 664
           NLE + L  C++L+ +                   S ++FP  +  +SL+ L LR C +L
Sbjct: 647 NLEYVNLYQCSNLEEVHHSLGCCSKVIGLYLNDCKSLKRFPC-VNVESLEYLGLRSCDSL 706

Query: 665 EEITDFSMASNLEILDLNTCFSLRIIHESIGSLDKLIT-LQLDLCHNLEKLPSSL-KLKS 724
           E++ +       EI        +R +  SI      +T L L    NL  LPSS+ +LKS
Sbjct: 707 EKLPEIYGRMKPEIQIHMQGSGIRELPSSIFQYKTHVTKLLLWNMKNLVALPSSICRLKS 766

Query: 725 LDSLSFTNCYKLEQLPEFDENMKSLRVMNLNGTAIRVLPSSIGYLIGLENLNLNDCANLT 784
           L SLS + C KLE LPE   ++ +LRV + + T I   PSSI   I L  L +       
Sbjct: 767 LVSLSVSGCSKLESLPEEIGDLDNLRVFDASDTLILRPPSSI---IRLNKLII---LMFR 826

Query: 785 ALPNEIHWLKSLEELHLRGCSKLDMFPPRSSLNFSQESSYFKLTVLDLKNCNISNSDFLE 844
              + +H+                 FPP +            L  L+L  CN+ +    E
Sbjct: 827 GFKDGVHF----------------EFPPVAE-------GLHSLEYLNLSYCNLIDGGLPE 886

Query: 845 TLSNVCTSLEKLNLSGNKFSCLP-SLQNFKSLRFLELRNCKFLQNIIKLPHHLARVNASG 903
            + ++ +SL+KL+LS N F  LP S+    +L+ L+L++C+ L  + +LP          
Sbjct: 887 EIGSL-SSLKKLDLSRNNFEHLPSSIAQLGALQSLDLKDCQRLTQLPELP---------- 919

BLAST of Cucsa.275630 vs. Swiss-Prot
Match: TAO1_ARATH (Disease resistance protein TAO1 OS=Arabidopsis thaliana GN=TAO1 PE=3 SV=1)

HSP 1 Score: 457.2 bits (1175), Expect = 4.3e-127
Identity = 300/893 (33.59%), Postives = 478/893 (53.53%), Query Frame = 1

Query: 2   SSSSCSSNLK--WSYDVFLSFRGEDTRNNFTSHLDRALREKGVNFFIDDKLERGGQISES 61
           SSSS  S+L   W + VFLSFRGED R    SH+ +  +  G+  FID++++RGG I   
Sbjct: 26  SSSSPPSSLSQNWLHPVFLSFRGEDVRKGLLSHIQKEFQRNGITPFIDNEMKRGGSIGPE 85

Query: 62  LLKSIDGSKISIIIFSKNYASSTWCLDELVKIVQCMKSMGHIVFPVFYKVDPSEVRKQTG 121
           LL++I GSKI+II+ S+NY SS WCLDELV+I++C + +G  V  VFY VDPS+VRKQ G
Sbjct: 86  LLQAIRGSKIAIILLSRNYGSSKWCLDELVEIMKCREELGQTVMTVFYDVDPSDVRKQKG 145

Query: 122 GFGEALAKHEANELMTNKVQPWKEALTTAASLSGWDLATRKNEADLIHDLVKEVLSILNQ 181
            FG+   K          VQ WK+ALT+AA++ G D    +NEAD+I  + K+V  +L+ 
Sbjct: 146 DFGKVFKKTCVGR-PEEMVQRWKQALTSAANILGEDSRNWENEADMIIKISKDVSDVLSF 205

Query: 182 TQLLHVAKHPVGIDSQLRAVEELASHDVPDGVNMVGIHGMGGIGKTTLAKALYNKIAYQF 241
           T      +  VGI++    +  L   D+ + V M+GI G  GIGKTT+++ LYNK+ +QF
Sbjct: 206 TPSKDFDEF-VGIEAHTTEITSLLQLDLEE-VRMIGIWGPAGIGKTTISRVLYNKLFHQF 265

Query: 242 EACCFLSNV-----RETLEQFKDLVQLQEKLLSEILKDNAWKVGNVHKGKNIIRDRLCSK 301
           +    + N+     R   +++   +QLQ++LLS+++      V ++     + ++RL  K
Sbjct: 266 QLGAIIDNIKVRYPRPCHDEYSAKLQLQKELLSQMINQKDMVVPHL----GVAQERLKDK 325

Query: 302 KVLIILDDVDKDEQLDALVGERDWFGRGSKIIATTRDRHLLENHSFDIVYPIQLLDPKKS 361
           KVL++LDDVD   QLDA+  +  WFG GS+II  T+D  LL+ H    +Y +      ++
Sbjct: 326 KVLLVLDDVDGLVQLDAMAKDVQWFGLGSRIIVVTQDLKLLKAHGIKYIYKVDFPTSDEA 385

Query: 362 LELFSLHAFKQNHPSSNYVDLSKFAVSYCKGLPLALVILGSLLHKRERKIWKSKLHELEN 421
           LE+F ++AF +  P   +  +++   +    LPL L ++GS L +  ++ W   +  L  
Sbjct: 386 LEIFCMYAFGEKSPKVGFEQIARTVTTLAGKLPLGLRVMGSYLRRMSKQEWAKSIPRLRT 445

Query: 422 SLEPSVEAVFQIGFKELHERVKEIFLDISCFFVGEDINYSKDVLKACDLNPDYGIIILMD 481
           SL+  +E+V +  +  L E+ K++FL I+CFF  E I   +  L    ++   G+ IL D
Sbjct: 446 SLDDDIESVLKFSYNSLAEQEKDLFLHITCFFRRERIETLEVFLAKKSVDMRQGLQILAD 505

Query: 482 LSLVTVEDGKIQMHDLIQQMGQTIVRHESF-EPAKRSRLWEAEGAIKILKEKSVS----- 541
            SL+++  G I+MH+L+ Q+G  IVR +S  +P KR  L + E   ++L + + +     
Sbjct: 506 KSLLSLNLGNIEMHNLLVQLGLDIVRKQSIHKPGKRQFLVDTEDICEVLTDDTGTRTLIG 565

Query: 542 -DFRQCFYLLIAKDIYSEAFRNMKNLRLLILQR--------VAYFPRNIFEYLPNSLKWI 601
            D      +    +I   AF  M NL+ L            + Y P+ +  ++   L+ +
Sbjct: 566 IDLELSGVIEGVINISERAFERMCNLQFLRFHHPYGDRCHDILYLPQGL-SHISRKLRLL 625

Query: 602 EWSTFYVNQSSSISFSETPNFFATLNLEKLYLRGCTSLKGMRPSFRKFPSHLKFKSLKVL 661
            W  + +   + +     P F   +N+    L             + +  +   ++LK +
Sbjct: 626 HWERYPL---TCLPPKFNPEFLVKINMRDSMLE------------KLWDGNEPIRNLKWM 685

Query: 662 NLRDCLNLEEITDFSMASNLEILDLNTCFSLRIIHESIGSLDKLITLQLDLCHNLEKLPS 721
           +L  C+NL+E+ DFS A+NL+ L L  C SL  +  SIG+   L+ L L  C +L KLPS
Sbjct: 686 DLSFCVNLKELPDFSTATNLQELRLINCLSLVELPSSIGNATNLLELDLIDCSSLVKLPS 745

Query: 722 SL-KLKSLDSLSFTNCYKLEQLPEFDENMKSLRVMNLNG-TAIRVLPSSIGYLIGLENLN 781
           S+  L +L  L    C  L +LP    N+ SL+ +NL+G +++  +PSSIG ++ L+ + 
Sbjct: 746 SIGNLTNLKKLFLNRCSSLVKLPSSFGNVTSLKELNLSGCSSLLEIPSSIGNIVNLKKVY 805

Query: 782 LNDCANLTALPNEIHWLKSLEELHLRGCSKLDMFPPRSSLNFSQESSYFKLTVLDLKNCN 841
            + C++L  LP+ I    +L+ELHL  CS L M  P S LN ++           L++ N
Sbjct: 806 ADGCSSLVQLPSSIGNNTNLKELHLLNCSSL-MECPSSMLNLTR-----------LEDLN 863

Query: 842 ISNSDFLETLSNVCTSLEKLNLSGNKFSCLPSLQNFKSLRFLELRNCKFLQNI 871
           +S           C SL K          LPS+ N  +L+ L L +C  L  +
Sbjct: 866 LSG----------CLSLVK----------LPSIGNVINLQSLYLSDCSSLMEL 863

HSP 2 Score: 451.1 bits (1159), Expect = 3.1e-125
Identity = 315/979 (32.18%), Postives = 507/979 (51.79%), Query Frame = 1

Query: 2   SSSSCSSNLK--WSYDVFLSFRGEDTRNNFTSHLDRALREKGVNFFIDDKLERGGQISES 61
           SSSS  S+L   W + VFLSFRGED R    SH+ +  +  G+  FID++++RGG I   
Sbjct: 26  SSSSPPSSLSQNWLHPVFLSFRGEDVRKGLLSHIQKEFQRNGITPFIDNEMKRGGSIGPE 85

Query: 62  LLKSIDGSKISIIIFSKNYASSTWCLDELVKIVQCMKSMGHIVFPVFYKVDPSEVRKQTG 121
           LL++I GSKI+II+ S+NY SS WCLDELV+I++C + +G  V  VFY VDPS+VRKQ G
Sbjct: 86  LLQAIRGSKIAIILLSRNYGSSKWCLDELVEIMKCREELGQTVMTVFYDVDPSDVRKQKG 145

Query: 122 GFGEALAKHEANELMTNKVQPWKEALTTAASLSGWDLATRKNEADLIHDLVKEVLSILNQ 181
            FG+   K          VQ WK+ALT+AA++ G D    +NEAD+I  + K+V  +L+ 
Sbjct: 146 DFGKVFKKTCVGR-PEEMVQRWKQALTSAANILGEDSRNWENEADMIIKISKDVSDVLSF 205

Query: 182 TQLLHVAKHPVGIDSQLRAVEELASHDVPDGVNMVGIHGMGGIGKTTLAKALYNKIAYQF 241
           T      +  VGI++    +  L   D+ + V M+GI G  GIGKTT+++ LYNK+ +QF
Sbjct: 206 TPSKDFDEF-VGIEAHTTEITSLLQLDLEE-VRMIGIWGPAGIGKTTISRVLYNKLFHQF 265

Query: 242 EACCFLSNV-----RETLEQFKDLVQLQEKLLSEILKDNAWKVGNVHKGKNIIRDRLCSK 301
           +    + N+     R   +++   +QLQ++LLS+++      V ++     + ++RL  K
Sbjct: 266 QLGAIIDNIKVRYPRPCHDEYSAKLQLQKELLSQMINQKDMVVPHL----GVAQERLKDK 325

Query: 302 KVLIILDDVDKDEQLDALVGERDWFGRGSKIIATTRDRHLLENHSFDIVYPIQLLDPKKS 361
           KVL++LDDVD   QLDA+  +  WFG GS+II  T+D  LL+ H    +Y +      ++
Sbjct: 326 KVLLVLDDVDGLVQLDAMAKDVQWFGLGSRIIVVTQDLKLLKAHGIKYIYKVDFPTSDEA 385

Query: 362 LELFSLHAFKQNHPSSNYVDLSKFAVSYCKGLPLALVILGSLLHKRERKIWKSKLHELEN 421
           LE+F ++AF +  P   +  +++   +    LPL L ++GS L +  ++ W   +  L  
Sbjct: 386 LEIFCMYAFGEKSPKVGFEQIARTVTTLAGKLPLGLRVMGSYLRRMSKQEWAKSIPRLRT 445

Query: 422 SLEPSVEAVFQIGFKELHERVKEIFLDISCFFVGEDINYSKDVLKACDLNPDYGIIILMD 481
           SL+  +E+V +  +  L E+ K++FL I+CFF  E I   +  L    ++   G+ IL D
Sbjct: 446 SLDDDIESVLKFSYNSLAEQEKDLFLHITCFFRRERIETLEVFLAKKSVDMRQGLQILAD 505

Query: 482 LSLVTVEDGKIQMHDLIQQMGQTIVRHESF-EPAKRSRLWEAEGAIKILKEKSVS----- 541
            SL+++  G I+MH+L+ Q+G  IVR +S  +P KR  L + E   ++L + + +     
Sbjct: 506 KSLLSLNLGNIEMHNLLVQLGLDIVRKQSIHKPGKRQFLVDTEDICEVLTDDTGTRTLIG 565

Query: 542 -DFRQCFYLLIAKDIYSEAFRNMKNLRLLILQR--------VAYFPRNIFEYLPNSLKWI 601
            D      +    +I   AF  M NL+ L            + Y P+ +  ++   L+ +
Sbjct: 566 IDLELSGVIEGVINISERAFERMCNLQFLRFHHPYGDRCHDILYLPQGL-SHISRKLRLL 625

Query: 602 EWSTFYVNQSSSISFSETPNFFATLNLEKLYLRGCTSLKGMRPSFRKFPSHLKFKSLKVL 661
            W  + +   + +     P F   +N+    L             + +  +   ++LK +
Sbjct: 626 HWERYPL---TCLPPKFNPEFLVKINMRDSMLE------------KLWDGNEPIRNLKWM 685

Query: 662 NLRDCLNLEEITDFSMASNLEILDLNTCFSLRIIHESIGSLDKLITLQLDLCHNLEKLPS 721
           +L  C+NL+E+ DFS A+NL+ L L  C SL  +  SIG+   L+ L L  C +L KLPS
Sbjct: 686 DLSFCVNLKELPDFSTATNLQELRLINCLSLVELPSSIGNATNLLELDLIDCSSLVKLPS 745

Query: 722 SL-KLKSLDSLSFTNCYKLEQLPEFDENMKSLRVMNLNG--------------------- 781
           S+  L +L  L    C  L +LP    N+ SL+ +NL+G                     
Sbjct: 746 SIGNLTNLKKLFLNRCSSLVKLPSSFGNVTSLKELNLSGCSSLLEIPSSIGNIVNLKKVY 805

Query: 782 ----TAIRVLPSSIGYLIGLENLNLNDCANLTALPNEIHWLKSLEELHLRGCSKLDMFPP 841
               +++  LPSSIG    L+ L+L +C++L   P+ +  L  LE+L+L GC  L   P 
Sbjct: 806 ADGCSSLVQLPSSIGNNTNLKELHLLNCSSLMECPSSMLNLTRLEDLNLSGCLSLVKLPS 865

Query: 842 ------RSSLNFSQESSYFKLTVLDLKNCNISNSDFLETLSNV---------CTSLEKLN 901
                   SL  S  SS  +L    ++N    ++ +L+  SN+          T+L+ L 
Sbjct: 866 IGNVINLQSLYLSDCSSLMELP-FTIENATNLDTLYLDGCSNLLELPSSIWNITNLQSLY 925

Query: 902 LSG-NKFSCLPSL-QNFKSLRFLELRNCKFLQNIIKLPHHLARVN----------ASGSE 906
           L+G +    LPSL +N  +L+ L L  C    ++++LP  + R++          +S  E
Sbjct: 926 LNGCSSLKELPSLVENAINLQSLSLMKC---SSLVELPSSIWRISNLSYLDVSNCSSLLE 977

BLAST of Cucsa.275630 vs. Swiss-Prot
Match: Y4117_ARATH (Putative disease resistance protein At4g11170 OS=Arabidopsis thaliana GN=At4g11170 PE=2 SV=1)

HSP 1 Score: 432.6 bits (1111), Expect = 1.1e-119
Identity = 286/809 (35.35%), Postives = 438/809 (54.14%), Query Frame = 1

Query: 7   SSNLKWSYDVFLSFRGEDTRNNFTSHLDRALREKGVNFFIDDKLERGGQISESLLKSIDG 66
           SS+  W YDVF SFRGED RNNF SHL +    KG+  F DD ++R   I   L  +I  
Sbjct: 4   SSSNSWRYDVFPSFRGEDVRNNFLSHLLKEFESKGIVTFRDDHIKRSHTIGHELRAAIRE 63

Query: 67  SKISIIIFSKNYASSTWCLDELVKIVQCMKSMGHIVFPVFYKVDPSEVRKQTGGFGEALA 126
           SKIS+++FS+NYASS+WCLDEL++I++C +  G  V PVFYKVDPS++RKQTG FG +  
Sbjct: 64  SKISVVLFSENYASSSWCLDELIEIMKCKEEQGLKVMPVFYKVDPSDIRKQTGKFGMSFL 123

Query: 127 KHEANELMTNKVQPWKEALTTAASLSGWDLATRKNEADLIHDLVKEVLSILNQTQLLHVA 186
           +    +    +   W+ ALT AA++ G       NEA  I  + K+VL  LN T      
Sbjct: 124 ETCCGK-TEERQHNWRRALTDAANILGDHPQNWDNEAYKITTISKDVLEKLNATPSRDF- 183

Query: 187 KHPVGIDSQLRAVEELASHDVPDGVNMVGIHGMGGIGKTTLAKALYNKIAYQFEACCFLS 246
              VG+++ +  +E L   +   GV +VGI G  G+GKTT+A+ALYN+    F    F+ 
Sbjct: 184 NDLVGMEAHIAKMESLLCLE-SQGVRIVGIWGPAGVGKTTIARALYNQYHENFNLSIFME 243

Query: 247 NVRET-----LEQFKDLVQLQEKLLSEILKDNAWKVGNVHKGKNIIRDRLCSKKVLIILD 306
           NVRE+     L+ +   + LQ++ LS++L     +V ++      I +RL S+KVLIILD
Sbjct: 244 NVRESYGEAGLDDYGLKLHLQQRFLSKLLDQKDLRVRHL----GAIEERLKSQKVLIILD 303

Query: 307 DVDKDEQLDALVGERDWFGRGSKIIATTRDRHLLENHSFDIVYPIQLLDPKKSLELFSLH 366
           DVD  EQL AL  E  WFG  S+I+ TT+++ LL +H  + +Y +     +++L +F  H
Sbjct: 304 DVDNIEQLKALAKENQWFGNKSRIVVTTQNKQLLVSHDINHMYQVAYPSKQEALTIFCQH 363

Query: 367 AFKQNHPSSNYVDLSKFAVSYCKGLPLALVILGSLLHKRERKIWKSKLHELENSLEPSVE 426
           AFKQ+ PS +   L+    +    LPLAL +LGS +  + ++ W+  L  L++ L+  VE
Sbjct: 364 AFKQSSPSDDLKHLAIEFTTLAGHLPLALRVLGSFMRGKGKEEWEFSLPTLKSRLDGEVE 423

Query: 427 AVFQIGFKELHERVKEIFLDISCFFVGEDINYSKDVLKA-CDLNPDYGIIILMDLSLV-T 486
            V ++G+  LH+  K++FL I+C F G+  NY K ++ A  D    +G+ +L D SL+  
Sbjct: 424 KVLKVGYDGLHDHEKDLFLHIACIFSGQHENYLKQMIIANNDTYVSFGLQVLADKSLIQK 483

Query: 487 VEDGKIQMHDLIQQMGQTIVRHES-FEPAKRSRLWEAEGAIKILKEKSVSDFRQCFYL-- 546
            E+G+I+MH L++Q+G+ +VR +S +EP KR  L  A+    +L   + +       L  
Sbjct: 484 FENGRIEMHSLLRQLGKEVVRKQSIYEPGKRQFLMNAKETCGVLSNNTGTGTVLGISLDM 543

Query: 547 -LIAKDIY--SEAFRNMKNLRLL----------ILQRVAYFPRNIFEYLPNSLKWIEWST 606
             I +++Y   + F  M+NL  L           ++     P     YLP  L+ + W  
Sbjct: 544 CEIKEELYISEKTFEEMRNLVYLKFYMSSPIDDKMKVKLQLPEEGLSYLP-QLRLLHWDA 603

Query: 607 FYVNQSSSISFSETPNFFATLNLEKLYLRGCTSLKGMRPSFRKFPSHLKFKSLKVLNLRD 666
           + +    S   S  P     LN+    L+   S  G++P           ++L+ +NL  
Sbjct: 604 YPLEFFPS---SFRPECLVELNMSHSKLKKLWS--GVQP----------LRNLRTMNLNS 663

Query: 667 CLNLEEITDFSMASNLEILDLNTCFSLRIIHESIGSLDKLITLQLDLCHNLEKLPSSLKL 726
             NLE + +   A+ L  LDL  C SL  +  SI +L  LI L++  C  LE +P+++ L
Sbjct: 664 SRNLEILPNLMEATKLNRLDLGWCESLVELPSSIKNLQHLILLEMSCCKKLEIIPTNINL 723

Query: 727 KSLDSLSFTNCYKLEQLPEFDENMKSLRVMNLNGTAIRVLPSSIGYLIGLENLNLNDCAN 786
            SL+ L F  C +L+  PE   N   +R++NL GTAI  +P S+ Y   ++ +    C  
Sbjct: 724 PSLEVLHFRYCTRLQTFPEISTN---IRLLNLIGTAITEVPPSVKYWSKIDEI----CME 782

Query: 787 LTALPNEIHWLKSLEELHLRGCSKLDMFP 793
              +   +H    LE+L LR   +L+  P
Sbjct: 784 RAKVKRLVHVPYVLEKLCLRENKELETIP 782

BLAST of Cucsa.275630 vs. Swiss-Prot
Match: RLM1A_ARATH (Disease resistance protein RML1A OS=Arabidopsis thaliana GN=RLM1A PE=3 SV=1)

HSP 1 Score: 419.5 bits (1077), Expect = 1.0e-115
Identity = 280/827 (33.86%), Postives = 445/827 (53.81%), Query Frame = 1

Query: 2   SSSSCSSNLKWSYDVFLSFRGEDTRNNFTSHLDRALREKGVNFFIDDKLERGGQISESLL 61
           +SSS S++  W Y VF SF G D R +F SH  +     G+  F D ++ RG  IS +L 
Sbjct: 2   ASSSSSASRTWRYRVFTSFHGSDVRTSFLSHFRKQFNNNGITMFDDQRILRGETISPALT 61

Query: 62  KSIDGSKISIIIFSKNYASSTWCLDELVKIVQCMKSMGHIVFPVFYKVDPSEVRKQTGGF 121
           ++I  S+ISI++ SKNYASS WCLDEL++I++C   MG IV  VFY VDPS+VRKQTG F
Sbjct: 62  QAIRESRISIVLLSKNYASSGWCLDELLEILKCKDDMGQIVMTVFYGVDPSDVRKQTGEF 121

Query: 122 GEALAKHEANELMTNKV-QPWKEALTTAASLSGWDLATRKNEADLIHDLVKEVLSILNQT 181
           G  +A +E     T +  Q W +AL    +++G  L    NEA +I  + ++V   LN T
Sbjct: 122 G--IAFNETCACRTEEERQKWSQALNYVGNIAGEHLLNWDNEAKMIEKIARDVSEKLNVT 181

Query: 182 QLLHVAKHPVGIDSQLRAVEELASHDVPDGVNMVGIHGMGGIGKTTLAKALYNKIAYQFE 241
                    VGI++ LR ++ L   D  D V MV I G  GIGK+T+ +AL++ ++ +F 
Sbjct: 182 PCRDF-DGMVGIEAHLRKIQSLLDLD-NDEVKMVAISGPAGIGKSTIGRALHSLLSNRFH 241

Query: 242 ACCFLSNVRET----LEQFKDLVQLQEKLLSEILKDNAWKVGNVHKGKNIIRDRLCSKKV 301
             CF+ N+R +    L+++   ++LQE+LLS+IL  +  ++   H G   I++RLC  KV
Sbjct: 242 HTCFVDNLRGSHPIGLDEYGLKLRLQEQLLSKILNQDGSRI--CHLG--AIKERLCDMKV 301

Query: 302 LIILDDVDKDEQLDALVGERDWFGRGSKIIATTRDRHLLENHSFDIVYPIQLLDPKKSLE 361
            IILDDV+  +QL+AL  E +WFG GS+II TT ++ LL+ H  +  Y +     +++++
Sbjct: 302 FIILDDVNDVKQLEALANESNWFGPGSRIIVTTENKELLKQHGINNTYYVGFPSDEEAIK 361

Query: 362 LFSLHAFKQNHPSSNYVDLSKFAVSYCKGLPLALVILGSLLHKRERKIWKSKLHELENSL 421
           +   +AF+Q+     +  L++     C  LPL L ++GS LH +  + W+  +  LE  +
Sbjct: 362 ILCRYAFRQSSSRHGFKKLTRSVTELCGKLPLGLRVVGSSLHGKNEEEWEYVIRRLETII 421

Query: 422 EPSVEAVFQIGFKELHERVKEIFLDISCFFVGEDINYSKDVLKACDLNPDYGIIILMDLS 481
           +  +E V ++G++ LHE  + +FL I+ FF  ED +  K +L   DL+ ++ + IL++ S
Sbjct: 422 DRDIEQVLRVGYESLHENEQSLFLHIAIFFNYEDGDLVKAMLAENDLDIEHELNILVNKS 481

Query: 482 LVTVE-DGKIQMHDLIQQMGQTIVRHESFEPAKRSRLWEAEGAIKILKEK----SVSDFR 541
           L+ +  DG+I+MH L+Q +G+   + E  EP KR  L +A+    +L+      +VS   
Sbjct: 482 LIYISTDGRIRMHKLLQLVGRQANQRE--EPWKRRILIDAQEICHVLENDIGTGAVSGIL 541

Query: 542 QCFYLLIAKDIYSEAFRNMKNLRLLILQRVAYFPRNIFEY-----LPNSLKWIEWSTFYV 601
                +    I ++A R M NLR L + +  +   N  +       P  L+ + W  +  
Sbjct: 542 FDTSGINEVSISNKALRRMCNLRFLSVYKTKHDGYNRMDIPEDMEFPPRLRLLHWDAY-- 601

Query: 602 NQSSSISFSETPNFFATLNLEKLYLRGCTSLKGMRPSFRKFPSHLKFKSLKVLNLRDCLN 661
             S  +     P  F   NL +L       +K  R  +  +P       LK LNL    N
Sbjct: 602 -PSKCL-----PLKFRAENLVEL------DMKDSRLEY-LWPGTQLLTKLKKLNLEGSYN 661

Query: 662 LEEITDFSMASNLEILDLNTCFSLRIIHESIGSLDKLITLQLDLCHNLEKLPSSLKLKSL 721
           L+E+ D S A+NLE+LDL+ C +L  +  SI +L KL  + +DLC +L  +P+++ L SL
Sbjct: 662 LKELPDLSNATNLEMLDLSVCLALAELPSSIKNLHKLDVIYMDLCESLHMIPTNINLASL 721

Query: 722 DSLSFTNCYKLEQLPEFDENMKSLRVMNLNGTAIRVLPSSIGYLIGLENLNLNDCANL-- 781
           +++  T C +L+  P F   +K L ++    T +  +P+SI +   L  ++L+   NL  
Sbjct: 722 ETMYMTGCPQLKTFPAFSTKIKRLYLVR---TGVEEVPASITHCSRLLKIDLSGSRNLKS 781

Query: 782 -TALPNEIHW------------------LKSLEELHLRGCSKLDMFP 793
            T LP+ +                    L+ L+ L L  C KL   P
Sbjct: 782 ITHLPSSLQTLDLSSTDIEMIADSCIKDLQRLDHLRLCRCRKLKSLP 800

BLAST of Cucsa.275630 vs. Swiss-Prot
Match: RPP1_ARATH (Probable disease resistance protein RPP1 OS=Arabidopsis thaliana GN=RPP1 PE=2 SV=1)

HSP 1 Score: 413.3 bits (1061), Expect = 7.2e-114
Identity = 273/897 (30.43%), Postives = 467/897 (52.06%), Query Frame = 1

Query: 3   SSSCSSNLKWSYDVFLSFRGEDTRNNFTSHLDRALREKGVNFFIDDKLERGGQISESLLK 62
           S + S +  W +DVF SF G D R  F SH+  + R KG++ FID+ +ER   I   L +
Sbjct: 87  SPATSVSRNWKHDVFPSFHGADVRRTFLSHIMESFRRKGIDTFIDNNIERSKSIGPELKE 146

Query: 63  SIDGSKISIIIFSKNYASSTWCLDELVKIVQCMKSMGHIVFPVFYKVDPSEVRKQTGGFG 122
           +I GSKI+I++ S+ YASS+WCLDEL +I++C + +G IV  +FY+VDP++++KQTG FG
Sbjct: 147 AIKGSKIAIVLLSRKYASSSWCLDELAEIMKCRQMVGQIVMTIFYEVDPTDIKKQTGEFG 206

Query: 123 EALAKHEANELMTNKVQPWKEALTTAASLSGWDLATRKNEADLIHDLVKEVLSILNQTQL 182
           +A  K         +V+ W++AL   A+++G+   + +NEAD+I  +  +V ++LN    
Sbjct: 207 KAFTK-TCRGKPKEQVERWRKALEDVATIAGYHSHSWRNEADMIEKISTDVSNMLNSFTP 266

Query: 183 LHVAKHPVGIDSQLRAVEELASHDVPDGVNMVGIHGMGGIGKTTLAKALYNKIAYQFEAC 242
                  VG+ + +  +E+L   D+ D V M+GI G  GIGKTT+A+ L+N+++ +F+  
Sbjct: 267 SRDFDGLVGMRAHMDMLEQLLRLDL-DEVRMIGIWGPPGIGKTTIARFLFNQVSDRFQLS 326

Query: 243 CFLSNV-----RETLEQFKDLVQLQEKLLSEILKDNAWKVGNVHKGKNIIRDRLCSKKVL 302
             + N+     R   +++   +QLQ ++LS+++      + ++     + ++RL  KKV 
Sbjct: 327 AIMVNIKGCYPRPCFDEYSAQLQLQNQMLSQMINHKDIMISHL----GVAQERLRDKKVF 386

Query: 303 IILDDVDKDEQLDALVGERDWFGRGSKIIATTRDRHLLENHSFDIVYPIQLLDPKKSLEL 362
           ++LD+VD+  QLDAL  E  WFG GS+II TT D  +L+ H  + VY ++     ++ ++
Sbjct: 387 LVLDEVDQLGQLDALAKETRWFGPGSRIIITTEDLGVLKAHGINHVYKVEYPSNDEAFQI 446

Query: 363 FSLHAFKQNHPSSNYVDLSKFAVSYCKGLPLALVILGSLLHKRERKIWKSKLHELENSLE 422
           F ++AF Q  P   + +++         LPL L +LGS L  + ++ W+  L  L+ SL+
Sbjct: 447 FCMNAFGQKQPHEGFDEIAWEVTCLAGELPLGLKVLGSALRGKSKREWERTLPRLKTSLD 506

Query: 423 PSVEAVFQIGFKELHERVKEIFLDISCFFVGEDINYSKDVLKACDLNPDYGIIILMDLSL 482
             + ++ Q  +  L +  K +FL I+C F GE     K++L    L+   G+ +L   SL
Sbjct: 507 GKIGSIIQFSYDVLCDEDKYLFLYIACLFNGESTTKVKELLGKF-LDVKQGLHLLAQKSL 566

Query: 483 VTVEDGKIQMHDLIQQMGQTIVR----HESFEPAKRSRLWEAEGAIKILKEKSVSDFRQC 542
           ++ +  +I MH L++Q G+   R    H  F   KR  L  A G  ++L + +    R  
Sbjct: 567 ISFDGERIHMHTLLEQFGRETSRKQFVHHGF--TKRQLLVGARGICEVLDDDTTDSRRFI 626

Query: 543 FYLLIAKDIYSEAFRNMKNLRLLILQRV--AYFPRNIFEYLPNSLKW-----------IE 602
              L   +   E      N+   +L+RV   +F R    + P  L+            I 
Sbjct: 627 GIHLELSNTEEEL-----NISEKVLERVHDFHFVRIDASFQPERLQLALQDLIYHSPKIR 686

Query: 603 WSTFYVNQSSSISFSETPNFFATLNLEKLYLRGCTSLKGMRPSFRKFPSHLKFKSLKVLN 662
              +Y  +S  +  +  P F   L++    LR            + +    + ++LK ++
Sbjct: 687 SLNWYGYESLCLPSTFNPEFLVELDMRSSNLR------------KLWEGTKQLRNLKWMD 746

Query: 663 LRDCLNLEEITDFSMASNLEILDLNTCFSLRIIHESIGSLDKLITLQLDLCHNLEKLPSS 722
           L     L+E+ + S A+NLE L L  C SL  +  SI  L  L  L L+ C +LEKLP+ 
Sbjct: 747 LSYSSYLKELPNLSTATNLEELKLRNCSSLVELPSSIEKLTSLQILDLENCSSLEKLPAI 806

Query: 723 LKLKSLDSLSFTNCYKLEQLPEFDENMKSLRVMNLNGTAIRV-LPSSIGYLIGLENLNLN 782
                L  L   NC  L +LP       +L+ +N++G +  V LPSSIG +  LE  +L+
Sbjct: 807 ENATKLRELKLQNCSSLIELPLSIGTATNLKQLNISGCSSLVKLPSSIGDITDLEVFDLS 866

Query: 783 DCANLTALPNEIHWLKSLEELHLRGCSKLDMFPPRSSLNFSQESSYFKLTVLDLKNCNIS 842
           +C++L  LP+ I  L++L +L +RGCSKL+  P               + +  L   N++
Sbjct: 867 NCSSLVTLPSSIGNLQNLCKLIMRGCSKLEALP-------------ININLKSLDTLNLT 926

Query: 843 NSDFLETLSNVCTSLEKLNLSGNKFSCLP-SLQNFKSLRFLELRNCKFLQNIIKLPH 876
           +   L++   + T + +L L G     +P S+ ++  L   ++    + +++++ PH
Sbjct: 927 DCSQLKSFPEISTHISELRLKGTAIKEVPLSIMSWSPLADFQI---SYFESLMEFPH 941

HSP 2 Score: 105.9 bits (263), Expect = 2.4e-21
Identity = 98/322 (30.43%), Postives = 152/322 (47.20%), Query Frame = 1

Query: 575  SLKWIEWSTFYVNQSSSISFSETPNFFATLNLEKLYLRGCTSLKGMRPSFRKFPSHLKFK 634
            +LKW++ S        S    E PN     NLE+L LR C+SL  +  S  K        
Sbjct: 715  NLKWMDLSY-------SSYLKELPNLSTATNLEELKLRNCSSLVELPSSIEKLT------ 774

Query: 635  SLKVLNLRDCLNLEEITDFSMASNLEILDLNTCFSLRIIHESIGSLDKLITLQLDLCHNL 694
            SL++L+L +C +LE++     A+ L  L L  C SL  +  SIG+   L  L +  C +L
Sbjct: 775  SLQILDLENCSSLEKLPAIENATKLRELKLQNCSSLIELPLSIGTATNLKQLNISGCSSL 834

Query: 695  EKLPSSL-KLKSLDSLSFTNCYKLEQLPEFDENMKSLRVMNLNG-TAIRVLPSSIGYLIG 754
             KLPSS+  +  L+    +NC  L  LP    N+++L  + + G + +  LP +I  L  
Sbjct: 835  VKLPSSIGDITDLEVFDLSNCSSLVTLPSSIGNLQNLCKLIMRGCSKLEALPININ-LKS 894

Query: 755  LENLNLNDCANLTALPNEIHWLKSLEELHLRGCSKLDMFPPRSSLNFSQESSYFKLTVLD 814
            L+ LNL DC+ L + P EI     + EL L+G +  ++  P S +++S            
Sbjct: 895  LDTLNLTDCSQLKSFP-EIS--THISELRLKGTAIKEV--PLSIMSWS-----------P 954

Query: 815  LKNCNISNSDFLETLSNVCTSLEKLNLSGNKFSCLPSLQNFKSLRFLELRNCKFLQNIIK 874
            L +  IS  + L    +    + KL+LS +     P ++    LR L L NC  L ++ +
Sbjct: 955  LADFQISYFESLMEFPHAFDIITKLHLSKDIQEVPPWVKRMSRLRDLSLNNCNNLVSLPQ 1005

Query: 875  LPHHLARVNASGSELLAIRPDC 895
            L   L  + A   + L  R DC
Sbjct: 1015 LSDSLDYIYADNCKSLE-RLDC 1005

HSP 3 Score: 89.4 bits (220), Expect = 2.4e-16
Identity = 92/340 (27.06%), Postives = 151/340 (44.41%), Query Frame = 1

Query: 511  RLWEAEGAIKILKEKSVSDFRQCFYLLIAKDIYSEAFRNMKNLRLLI-LQRVAYFPRNIF 570
            +LWE    ++ LK   +S              YS   + + NL     L+ +     +  
Sbjct: 705  KLWEGTKQLRNLKWMDLS--------------YSSYLKELPNLSTATNLEELKLRNCSSL 764

Query: 571  EYLPNSLKWIEWSTFYVNQSSSISFSETPNFFATLNLEKLYLRGCTSLKGMRPSFRKFPS 630
              LP+S++ +  S   ++  +  S  + P       L +L L+ C+SL  +  S     +
Sbjct: 765  VELPSSIEKLT-SLQILDLENCSSLEKLPAIENATKLRELKLQNCSSLIELPLSIGTATN 824

Query: 631  HLKFKSLKVLNLRDCLNLEEI-TDFSMASNLEILDLNTCFSLRIIHESIGSLDKLITLQL 690
                  LK LN+  C +L ++ +     ++LE+ DL+ C SL  +  SIG+L  L  L +
Sbjct: 825  ------LKQLNISGCSSLVKLPSSIGDITDLEVFDLSNCSSLVTLPSSIGNLQNLCKLIM 884

Query: 691  DLCHNLEKLPSSLKLKSLDSLSFTNCYKLEQLPEFDENMKSLRVMNLNGTAIRVLPSSIG 750
              C  LE LP ++ LKSLD+L+ T+C +L+  PE   ++  LR   L GTAI+ +P SI 
Sbjct: 885  RGCSKLEALPININLKSLDTLNLTDCSQLKSFPEISTHISELR---LKGTAIKEVPLSIM 944

Query: 751  YLIGLENLNLNDCANLTALPNEIHWLKSLEELHLRGCSKLDMFPPRSSLNFSQESSYFKL 810
                L +  ++   +L   P   H    + +LHL     +   PP             +L
Sbjct: 945  SWSPLADFQISYFESLMEFP---HAFDIITKLHL--SKDIQEVPP-------WVKRMSRL 1004

Query: 811  TVLDLKNCN--ISNSDFLETLSNV----CTSLEKLNLSGN 843
              L L NCN  +S     ++L  +    C SLE+L+   N
Sbjct: 1005 RDLSLNNCNNLVSLPQLSDSLDYIYADNCKSLERLDCCFN 1008

HSP 4 Score: 54.7 bits (130), Expect = 6.5e-06
Identity = 77/316 (24.37%), Postives = 130/316 (41.14%), Query Frame = 1

Query: 607 EKLYLRGCTSLKGMRPSFRKFPSHLKFKSLKVLNLRDCLNL--EEITDFSMASNLEILDL 666
           E++++       G   S ++F  H   K   ++  R    +  ++ TD      + +   
Sbjct: 565 ERIHMHTLLEQFGRETSRKQFVHHGFTKRQLLVGARGICEVLDDDTTDSRRFIGIHLELS 624

Query: 667 NTCFSLRIIHESIGSLDKLITLQLDLCHNLEKLPSSL--------KLKSLDSLSFTN-CY 726
           NT   L I  + +  +     +++D     E+L  +L        K++SL+   + + C 
Sbjct: 625 NTEEELNISEKVLERVHDFHFVRIDASFQPERLQLALQDLIYHSPKIRSLNWYGYESLCL 684

Query: 727 KLEQLPEFDENMKSLRVMNLNGTAIRVLPSSIGYLIGLENLNLNDCANLTALPNEIHWLK 786
                PEF      L  +++  + +R L      L  L+ ++L+  + L  LPN +    
Sbjct: 685 PSTFNPEF------LVELDMRSSNLRKLWEGTKQLRNLKWMDLSYSSYLKELPN-LSTAT 744

Query: 787 SLEELHLRGCSKLDMFPPRSSLNFSQESSYFKLTVLDLKNCNISNSDFLETLSNVCTSLE 846
           +LEEL LR CS L   P       S       L +LDL+NC+               SLE
Sbjct: 745 NLEELKLRNCSSLVELP-------SSIEKLTSLQILDLENCS---------------SLE 804

Query: 847 KLNLSGNKFSCLPSLQNFKSLRFLELRNCKFLQNIIKLP------HHLARVNASGSELLA 906
           KL          P+++N   LR L+L+NC    ++I+LP       +L ++N SG   L 
Sbjct: 805 KL----------PAIENATKLRELKLQNC---SSLIELPLSIGTATNLKQLNISGCSSLV 838

BLAST of Cucsa.275630 vs. TrEMBL
Match: A0A0A0KNK0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G494390 PE=4 SV=1)

HSP 1 Score: 1257.3 bits (3252), Expect = 0.0e+00
Identity = 695/952 (73.00%), Postives = 752/952 (78.99%), Query Frame = 1

Query: 1   QSSSSCSSNLKWSYDVFLSFRGEDTRNNFTSHLDRALREKGVNFFIDDKLERGGQISESL 60
           QSSSSCSSNLKWSYDVFLSFRGEDTRNNFTSHLDRALREKGVNFFIDDKLERGGQISESL
Sbjct: 10  QSSSSCSSNLKWSYDVFLSFRGEDTRNNFTSHLDRALREKGVNFFIDDKLERGGQISESL 69

Query: 61  LKSIDGSKISIIIFSKNYASSTWCLDELVKIVQCMKSMGHIVFPVFYKVDPSEVRKQTGG 120
           LKSIDGSKISIIIFSKNYASSTWCLDELVKIVQCMKSMGHIVFPVFYKVDPSEVRKQTGG
Sbjct: 70  LKSIDGSKISIIIFSKNYASSTWCLDELVKIVQCMKSMGHIVFPVFYKVDPSEVRKQTGG 129

Query: 121 FGEALAKHEANELMTNKVQPWKEALTTAASLSGWDLATRKNEADLIHDLVKEVLSILNQT 180
           FGEALAKHEANELMTNKVQPWKEALTTAASLSGWDLATRKNEADLIHDLVKEVLSILNQT
Sbjct: 130 FGEALAKHEANELMTNKVQPWKEALTTAASLSGWDLATRKNEADLIHDLVKEVLSILNQT 189

Query: 181 QLLHVAKHPVGIDSQLRAVEELASHDVPDGVNMVGIHGMGGIGKTTLAKALYNKIAYQFE 240
           QLLHVAKHPVGIDSQLRAVEELASHDVPDGVNMVGIHGMGGIGKTTLAKALYNKIAYQFE
Sbjct: 190 QLLHVAKHPVGIDSQLRAVEELASHDVPDGVNMVGIHGMGGIGKTTLAKALYNKIAYQFE 249

Query: 241 ACCFLSNVRETLEQFKDLVQLQEKLLSEILKDNAWKVGNVHKGKNIIRDRLCSKKVLIIL 300
           ACCFLSNVRETLEQFKDLVQLQEKLLSEILKDNAWKVGNVHKGKNIIRDRLCSKKVLIIL
Sbjct: 250 ACCFLSNVRETLEQFKDLVQLQEKLLSEILKDNAWKVGNVHKGKNIIRDRLCSKKVLIIL 309

Query: 301 DDVDKDEQLDALVGERDWFGRGSKIIATTRDRHLLENHSFDIVYPIQLLDPKKSLELFSL 360
           DDVDKDEQLDALVGERDWFGRGSKIIATTRDRHLLENHSFDIVYPIQLLDPKKSLELFSL
Sbjct: 310 DDVDKDEQLDALVGERDWFGRGSKIIATTRDRHLLENHSFDIVYPIQLLDPKKSLELFSL 369

Query: 361 HAFKQNHPSSNYVDLSKFAVSYCKGLPLALVILGSLLHKRERKIWKSKLHELENSLEPSV 420
           HAFKQNHPSSNYVDLSKFAVSYCKGLPLALVILGSLLHKRERKIWKSKLHELENSLEPSV
Sbjct: 370 HAFKQNHPSSNYVDLSKFAVSYCKGLPLALVILGSLLHKRERKIWKSKLHELENSLEPSV 429

Query: 421 EAVFQIGFKELHERVKEIFLDISCFFVGEDINYSKDVLKACDLNPDYGIIILMDLSLVTV 480
           EAVFQIGFKELHERVKEIFLDISCFFVGEDINYSKDVLKACDLNPDYGIIILMDLSLVTV
Sbjct: 430 EAVFQIGFKELHERVKEIFLDISCFFVGEDINYSKDVLKACDLNPDYGIIILMDLSLVTV 489

Query: 481 EDGKIQMHDLIQQMGQTIVRHESFEPAKRSRLWEAEGAIKILKEKSVSDFRQCFYLLI-- 540
           EDGKIQMHDLIQQMGQTIVRHESFEPAKRSRLWEAEGAIKILKEKS +   +   L +  
Sbjct: 490 EDGKIQMHDLIQQMGQTIVRHESFEPAKRSRLWEAEGAIKILKEKSGTKAVKAIKLDLHY 549

Query: 541 ---AKDIYSEAFRNMKNLRLLILQRVAYFPRNIFEYLPNSLKWIEWSTFYVNQSSSISFS 600
               K + +EAFRNMKNLRLLILQRVAYFP+NIFEYLPNSLKWIEWSTFYVNQSSSISFS
Sbjct: 550 KPWLKIVEAEAFRNMKNLRLLILQRVAYFPKNIFEYLPNSLKWIEWSTFYVNQSSSISFS 609

Query: 601 ETPNFFATLNLEKLYLRGCTSLKGMRPSFRKFPSHLKFKSLKVLNLRDCLNLEEITDFSM 660
                     L  L ++G  + K  R +F         K++K ++L  C  L+E  +FS 
Sbjct: 610 ------VKGRLVGLVMKGVVN-KQPRIAFE------NCKTMKHVDLSYCGTLKETPNFSA 669

Query: 661 ASNLEILDLNTCFSLRIIHESIGSLDKLITLQLDLCHNLEKLPSS-LKLKSLDSLSFTNC 720
             NLE L L  C SL++IHES+ SL KL+TL L+ C NLEK PSS L LKSL+ L+ + C
Sbjct: 670 TLNLEKLYLRGCTSLKVIHESVASLSKLVTLDLEGCDNLEKFPSSYLMLKSLEVLNLSRC 729

Query: 721 YKLEQLPEF--DENMKSLRVMNLNGTAIRVLPSSIG-YLIGLENLNLNDCANLTALPNEI 780
            K+E++P+     N+K L +   +   +R++  SIG  L  L  L+L  C NL  LP   
Sbjct: 730 RKIEEIPDLSASSNLKELYLRECD--RLRIIHDSIGRSLDKLIILDLEGCKNLERLPIYT 789

Query: 781 HWLKSLEELHLRGCSKLDMFPPRSSLNFSQESSYFKLTVLDLKNC-------NISNSDFL 840
           + L+SLE L+L  C KL+ F   S   F     +  L VL+L++C       + S +  L
Sbjct: 790 NKLESLELLNLASCLKLETFFDSSFRKFPSHLKFKSLKVLNLRDCLNLEEITDFSMASNL 849

Query: 841 ETLS-NVCTSLE---------------KLNLSGNKFSCLPSLQNFKSLRFLELRNCKFLQ 900
           E L  N C SL                +L+L  N    LPS    KSL  L   NC  L+
Sbjct: 850 EILDLNTCFSLRIIHESIGSLDKLITLQLDLCHN-LEKLPSSLKLKSLDSLSFTNCYKLE 909

Query: 901 NIIKLPHHLARVNASGSELLAIR--PDCIADMI---YLHANDRHHIKVLFPN 916
            + +   ++  +        AIR  P  I  +I    L+ ND  ++  L PN
Sbjct: 910 QLPEFDENMKSLRVMNLNGTAIRVLPSSIGYLIGLENLNLNDCANLTAL-PN 944

BLAST of Cucsa.275630 vs. TrEMBL
Match: A0A0A0KNK0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G494390 PE=4 SV=1)

HSP 1 Score: 555.8 bits (1431), Expect = 9.9e-155
Identity = 290/334 (86.83%), Postives = 301/334 (90.12%), Query Frame = 1

Query: 605  NLEKLYLRGCTSLKGM-RPSFRKFPSHLKFKSLKVLNLRDCLNLEEITDFSMASNLEILD 664
            +LE L L  C  L+     SFRKFPSHLKFKSLKVLNLRDCLNLEEITDFSMASNLEILD
Sbjct: 779  SLELLNLASCLKLETFFDSSFRKFPSHLKFKSLKVLNLRDCLNLEEITDFSMASNLEILD 838

Query: 665  LNTCFSLRIIHESIGSLDKLITLQLDLCHNLEKLPSSLKLKSLDSLSFTNCYKLEQLPEF 724
            LNTCFSLRIIHESIGSLDKLITLQLDLCHNLEKLPSSLKLKSLDSLSFTNCYKLEQLPEF
Sbjct: 839  LNTCFSLRIIHESIGSLDKLITLQLDLCHNLEKLPSSLKLKSLDSLSFTNCYKLEQLPEF 898

Query: 725  DENMKSLRVMNLNGTAIRVLPSSIGYLIGLENLNLNDCANLTALPNEIHWLKSLEELHLR 784
            DENMKSLRVMNLNGTAIRVLPSSIGYLIGLENLNLNDCANLTALPNEIHWLKSLEELHLR
Sbjct: 899  DENMKSLRVMNLNGTAIRVLPSSIGYLIGLENLNLNDCANLTALPNEIHWLKSLEELHLR 958

Query: 785  GCSKLDMFPPRSSLNFSQESSYFKLTVLDLKNCNISNSDFLETLSNVCTSLEKLNLSGNK 844
            GCSKLDMFPPRSSLNFSQESSYFKLTVLDLKNCNISNSDFLETLSNVCTSLEKLNLSGN 
Sbjct: 959  GCSKLDMFPPRSSLNFSQESSYFKLTVLDLKNCNISNSDFLETLSNVCTSLEKLNLSGNT 1018

Query: 845  FSCLPSLQNFKSLRFLELRNCKFLQNIIKLPHHLARVNASGSELLAIRPDCIADMIY--L 904
            FSCLPSLQNFKSLRFLELRNCKFLQNIIKLPHHLARVNASGSELLAIRPDCIADM++   
Sbjct: 1019 FSCLPSLQNFKSLRFLELRNCKFLQNIIKLPHHLARVNASGSELLAIRPDCIADMMFGKQ 1078

Query: 905  HANDRHHIKVLF--PNTTSKFVSKRFKSANVIMR 934
             A      KVLF   N   K+ +K+   +++ +R
Sbjct: 1079 DAEFSDSTKVLFITNNEIPKYCNKQTTRSSMSVR 1112

HSP 2 Score: 921.8 bits (2381), Expect = 6.9e-265
Identity = 537/928 (57.87%), Postives = 643/928 (69.29%), Query Frame = 1

Query: 1   QSSSSCSSNLKWSYDVFLSFRGEDTRNNFTSHLDRALREKGVNFFIDDKLERGGQISESL 60
           +SSSSCSSN KWSYDVFLSFRGEDTR+ F SHLD ALR +GVNFFIDDKL+RG QIS+SL
Sbjct: 10  ESSSSCSSNSKWSYDVFLSFRGEDTRDKFISHLDLALRREGVNFFIDDKLDRGKQISKSL 69

Query: 61  LKSIDGSKISIIIFSKNYASSTWCLDELVKIVQCMKSMGHIVFPVFYKVDPSEVRKQTGG 120
           LKSI+GS+ISIIIFS+NYASSTWCLDE+VKI++CM+S    V PVFY V PSEV KQTG 
Sbjct: 70  LKSIEGSRISIIIFSQNYASSTWCLDEVVKIIECMRSKKQTVLPVFYNVSPSEVVKQTGI 129

Query: 121 FGEALAKHEANELMTNKVQPWKEALTTAASLSGWDLAT--RKNEADLIHDLVKEVLSILN 180
           FGEA AK+E N LMTNK+QPWKEALTTAA+LSGWDL    + NEA LI DLVK+V SIL 
Sbjct: 130 FGEAFAKYETNPLMTNKIQPWKEALTTAATLSGWDLGNYWKNNEAHLIQDLVKKV-SILK 189

Query: 181 QTQLLHVAKHPVGIDSQLRAVEELASHDVPD-GVNMVGIHGMGGIGKTTLAKALYNKIAY 240
           QTQLL+VAKHPV IDSQL+A+EELASH V D GVNMVGIHGMGGIGKTTLAKALYNKI Y
Sbjct: 190 QTQLLNVAKHPVAIDSQLKAIEELASHGVSDNGVNMVGIHGMGGIGKTTLAKALYNKITY 249

Query: 241 QFEACCFLSNVRETLEQFKDLVQLQEKLLSEILKDNAWKVGNVHKGKNIIRDRLCSKKVL 300
           QFEACCFLSNVRET EQF  LVQLQEKLL+EI KDN  KV NV KG NII+DRLCS+KVL
Sbjct: 250 QFEACCFLSNVRETSEQFNGLVQLQEKLLNEIFKDNNLKVDNVDKGMNIIKDRLCSRKVL 309

Query: 301 IILDDVDKDEQLDALVGERDWFGRGSKIIATTRDRHLLENHSFDIVYPIQLLDPKKSLEL 360
           ++LDDVDKD+QLDALVG RDWFGRGSKII TTRDRHLLE +SFD ++PIQLLD  KSLEL
Sbjct: 310 MVLDDVDKDDQLDALVGGRDWFGRGSKIIVTTRDRHLLETYSFDKIHPIQLLDCDKSLEL 369

Query: 361 FSLHAFKQNHPSSNYVDLSKFAVSYCKGLPLALVILGSLLHKRERKIWKSKLHELENSLE 420
           F  HAFKQ+HPS NY +L +  V YC GLPLALVILGSLL KR++ IWKSKL EL+N  E
Sbjct: 370 FCWHAFKQSHPSRNYSELPEL-VRYCNGLPLALVILGSLLCKRDQIIWKSKLDELKNFPE 429

Query: 421 PSVEAVFQIGFKELHER--VKEIFLDISCFFVGEDINYSKDVLKACDLNPDYGIIILMDL 480
           P +EAVFQI FK L E   VKEIFLDI CFFVGED++YSK+VLKACD   +  IIILMDL
Sbjct: 430 PGIEAVFQISFKRLPENPPVKEIFLDICCFFVGEDVSYSKNVLKACDPYLESRIIILMDL 489

Query: 481 SLVTVEDGKIQMHDLIQQMGQTIVRHESFEPAKRSRLWEAEGAIKILKEKSVSDFRQCFY 540
           SLVTVEDGKIQMHDLI+QMGQ IVR +SF+P KRSRLW A+ A+K+L EKS +   +   
Sbjct: 490 SLVTVEDGKIQMHDLIRQMGQMIVRRKSFKPEKRSRLWVAKEAVKMLIEKSGTHKVKAIK 549

Query: 541 LLIAKD----IYSEAFRNMKNLRLLILQRVAYFPRNIFEYLPNSLKWIEWSTFYVNQSSS 600
           L +  +    + +EAFRNM+NLRLLILQ  A  P NIF+YLPN +KWIE+S      SSS
Sbjct: 550 LDLRNNGSLIVEAEAFRNMENLRLLILQNAAKLPTNIFKYLPN-IKWIEYS------SSS 609

Query: 601 ISFSETPNFFATLNLEKLYLRGCTSLKGMRPSFRKFPSHL--KFKSLKVLNLRDCLNLEE 660
           + +    +F     L  L + G ++         K P  +    K LK ++L     LEE
Sbjct: 610 VRWYFPISFVVNGGLVGLVINGVSN---------KHPGIIFEDCKMLKHVDLSYWRLLEE 669

Query: 661 ITDFSMASNLEILDLNTCFSLRIIHESIGSLDKLITLQLDLCHNLEKLPSS-LKLKSLDS 720
             DFS A NLE L L +C  L++IH S+ SL KL+TL L+ C NLEKLPSS L LKSL+ 
Sbjct: 670 TPDFSAALNLEKLYLLSCKRLKMIHGSVASLSKLVTLDLEGCENLEKLPSSFLMLKSLEV 729

Query: 721 LSFTNCYKLEQLPEF--DENMKSLRVMNLNGTAIRVLPSSIG-YLIGLENLNLNDCANLT 780
           L+ + C KL+++P+     N+K L +       I +  S++G +L  L  L+L  C  L 
Sbjct: 730 LNLSGCIKLKEIPDLSASSNLKELHLRECYHLRI-IHDSAVGRFLDKLVILDLEGCKILE 789

Query: 781 ALPNEIHWLKSLEELHLRGCSKLDMFPPRSSLNFSQESSYFKLTVLDLKNC-NISNSDFL 840
            LP  I   KS+E ++L  C K++         F     +  L VL+L  C N+      
Sbjct: 790 RLPRYISNSKSIEVMNLDSCRKIEQLFDNYFEKFPSHLKFESLKVLNLSYCQNLKEITDF 849

Query: 841 ETLSNV-------CTSLEKLNLS--------------GNKFSCLPSLQNFKSLRFLELRN 892
              SN+       C SL  ++ S               ++   LPS    KSL  L L N
Sbjct: 850 SIASNLEIFDLRGCFSLRTIHKSVGSLDQLIALKLDFCHQLEELPSCLRLKSLDSLSLTN 909

BLAST of Cucsa.275630 vs. TrEMBL
Match: A0A0A0LWV1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G334920 PE=4 SV=1)

HSP 1 Score: 409.8 bits (1052), Expect = 8.8e-111
Identity = 233/406 (57.39%), Postives = 278/406 (68.47%), Query Frame = 1

Query: 503  SFEPAKRSRLWEAEGAIKILKEKSVS--------DFRQCFYLLIAKDIYSEAFRNMKNLR 562
            SF   K   +    G IK+ +   +S          R+C++L I  D  S   R +  L 
Sbjct: 702  SFLMLKSLEVLNLSGCIKLKEIPDLSASSNLKELHLRECYHLRIIHD--SAVGRFLDKLV 761

Query: 563  LLILQRVAYFPRNIFEYLPNSLKWIEWSTFYVNQSSSISFSETPNFFATLNLEKLYLRGC 622
            +L L+        I E LP           Y++ S SI   E  N  +   +E+L+    
Sbjct: 762  ILDLEGC-----KILERLPR----------YISNSKSI---EVMNLDSCRKIEQLFDN-- 821

Query: 623  TSLKGMRPSFRKFPSHLKFKSLKVLNLRDCLNLEEITDFSMASNLEILDLNTCFSLRIIH 682
                     F KFPSHLKF+SLKVLNL  C NL+EITDFS+ASNLEI DL  CFSLR IH
Sbjct: 822  --------YFEKFPSHLKFESLKVLNLSYCQNLKEITDFSIASNLEIFDLRGCFSLRTIH 881

Query: 683  ESIGSLDKLITLQLDLCHNLEKLPSSLKLKSLDSLSFTNCYKLEQLPEFDENMKSLRVMN 742
            +S+GSLD+LI L+LD CH LE+LPS L+LKSLDSLS TNCYK+EQLPEFDENMKSLR MN
Sbjct: 882  KSVGSLDQLIALKLDFCHQLEELPSCLRLKSLDSLSLTNCYKIEQLPEFDENMKSLREMN 941

Query: 743  LNGTAIRVLPSSIGYLIGLENLNLNDCANLTALPNEIHWLKSLEELHLRGCSKLDMFPPR 802
            L GTAIR LP+SI YLIGLENL L+ C NL +LP+EIH LKSL+EL LR CS+LDM P  
Sbjct: 942  LKGTAIRKLPTSIRYLIGLENLILSYCTNLISLPSEIHLLKSLKELDLRECSRLDMLPSG 1001

Query: 803  SSLNFSQESSYFKLTVLDLKNCNISNSDFLETLSNVCTSLEKLNLSGNKFSCLPSLQNFK 862
            SSLNF Q S    LT+LDL+NCNISNSDFLE LSN CT+L++LNLSGNKF CLPSL+NF 
Sbjct: 1002 SSLNFPQRSLCSNLTILDLQNCNISNSDFLENLSNFCTTLKELNLSGNKFCCLPSLKNFT 1061

Query: 863  SLRFLELRNCKFLQNIIKLPHHLARVNASGSELLAIRPDCIADMIY 901
            SLR LELRNCKFL+NI+K+PH L R++ASG ELL I PD IADM++
Sbjct: 1062 SLRLLELRNCKFLRNIVKIPHCLKRMDASGCELLVISPDYIADMMF 1077

HSP 2 Score: 122.1 bits (305), Expect = 3.7e-24
Identity = 105/310 (33.87%), Postives = 147/310 (47.42%), Query Frame = 1

Query: 596 ETPNFFATLNLEKLYLRGCTSLKGMRPS-----------------FRKFP-SHLKFKSLK 655
           ETP+F A LNLEKLYL  C  LK +  S                   K P S L  KSL+
Sbjct: 651 ETPDFSAALNLEKLYLLSCKRLKMIHGSVASLSKLVTLDLEGCENLEKLPSSFLMLKSLE 710

Query: 656 VLNLRDCLNLEEITDFSMASNLEILDLNTCFSLRIIHES-IGS-LDKLITLQLDLCHNLE 715
           VLNL  C+ L+EI D S +SNL+ L L  C+ LRIIH+S +G  LDKL+ L L+ C  LE
Sbjct: 711 VLNLSGCIKLKEIPDLSASSNLKELHLRECYHLRIIHDSAVGRFLDKLVILDLEGCKILE 770

Query: 716 KLPSSL-KLKSLDSLSFTNCYKLEQL-----PEFDENMK--SLRVMNLNGTAIRVLPSSI 775
           +LP  +   KS++ ++  +C K+EQL      +F  ++K  SL+V+NL+        +  
Sbjct: 771 RLPRYISNSKSIEVMNLDSCRKIEQLFDNYFEKFPSHLKFESLKVLNLSYCQNLKEITDF 830

Query: 776 GYLIGLENLNLNDCANLTALPNEIHWLKSLEELHLRGCSKLDMFPPRSSLNFSQESSYFK 835
                LE  +L  C +L  +   +  L  L  L L  C +L+  P    L          
Sbjct: 831 SIASNLEIFDLRGCFSLRTIHKSVGSLDQLIALKLDFCHQLEELPSCLRLK--------S 890

Query: 836 LTVLDLKNCNISNSDFLETLSNVCTSLEKLNLSGNKFSCLPSLQNFKSLRFLELRNCKFL 878
           L  L L NC     + L        SL ++NL G     LP+  + + L  LE     + 
Sbjct: 891 LDSLSLTNC--YKIEQLPEFDENMKSLREMNLKGTAIRKLPT--SIRYLIGLENLILSYC 948

HSP 3 Score: 817.0 bits (2109), Expect = 2.4e-233
Identity = 486/900 (54.00%), Postives = 599/900 (66.56%), Query Frame = 1

Query: 1   QSSSSCSSNLKWSYDVFLSFRGEDTRNNFTSHLDRALREKGVNFFIDDKLERGGQISESL 60
           +SS+S SS+ KWS+DVFLSFRG+DTR+NFT HLD ALR+KGVN FIDD L+RG QISE+L
Sbjct: 9   ESSTSSSSSFKWSFDVFLSFRGDDTRSNFTGHLDMALRQKGVNVFIDDMLKRGEQISETL 68

Query: 61  LKSIDGSKISIIIFSKNYASSTWCLDELVKIVQCMKSMGHIVFPVFYKVDPSEVRKQTGG 120
            K+I  + ISI+IFS+NYASS+WCLDELVKIV+C KS G +V P+FYKVDPS+VRKQTG 
Sbjct: 69  SKAIQEALISIVIFSQNYASSSWCLDELVKIVECKKSKGQLVLPIFYKVDPSDVRKQTGC 128

Query: 121 FGEALAKHEANELMTNKVQPWKEALTTAASLSGWDLATRKNEADLIHDLVKEVLSILNQT 180
           FGEALAKH+AN +   K Q W++ALTT A+ SGWDL TRK EAD I DLVKEVLS LN  
Sbjct: 129 FGEALAKHQANFM--EKTQIWRDALTTVANFSGWDLGTRK-EADFIQDLVKEVLSRLNCA 188

Query: 181 Q-LLHVAKHPVGIDSQLRAVEELASH---DVPDGVNMVGIHGMGGIGKTTLAKALYNKIA 240
              L+VAK+PVGIDSQL  ++ L SH   D  DGV M+GI+G+GGIGKTTLAKALYNKIA
Sbjct: 189 NGQLYVAKYPVGIDSQLEDMK-LLSHQIRDAFDGVYMMGIYGIGGIGKTTLAKALYNKIA 248

Query: 241 YQFEACCFLSNVRETLEQFKDLVQLQEKLLSEILKDNAWKVGNVHKGKNIIRDRLCSKKV 300
            QFE  CFLSNVRET +QF  LVQLQEKLL EILK +  K+GN+ +G NIIR RL SKKV
Sbjct: 249 NQFEGFCFLSNVRETSKQFNGLVQLQEKLLYEILKFDL-KIGNLDEGINIIRSRLRSKKV 308

Query: 301 LIILDDVDKDEQLDALVGERDWFGRGSKIIATTRDRHLLENHSFDIVYPIQLLDPKKSLE 360
           LI+LDDVDK +QL+ALVGERDWFG GSKII TTR+ HLL +H FD  Y ++ L    SLE
Sbjct: 309 LIVLDDVDKLKQLEALVGERDWFGHGSKIIVTTRNSHLLSSHEFDEKYGVRELSHGHSLE 368

Query: 361 LFSLHAFKQNHPSSNYVDLSKFAVSYCKGLPLALVILGSLLHKRERKIWKSKLHELENSL 420
           LFS HAFK++HPSSNY+DLSK A +YCKG PLALV+LGS L  R++  W++ L E ENSL
Sbjct: 369 LFSWHAFKKSHPSSNYLDLSKRATNYCKGHPLALVVLGSFLCTRDQIKWRTILDEFENSL 428

Query: 421 EPSVEAVFQIGFKELHERVKEIFLDISCFFVGEDINYSKDVLKACDLNPDYGIIILMDLS 480
              +E + QI F  L E++KEIFLDISC FVGE +NY K VL  C  + D+GII+LMDLS
Sbjct: 429 SEDIEHIIQISFDGLEEKIKEIFLDISCLFVGEKVNYVKSVLNTCHFSLDFGIIVLMDLS 488

Query: 481 LVTVEDGKIQMHDLIQQMGQTIVRHESFEPAKRSRLWEAEGAIKILKEKSVSDFRQCFYL 540
           L+TVE+ ++QMHDLI+QMGQ IV  ESFEP KRSRLW     +K+  + S +   +   L
Sbjct: 489 LITVENEEVQMHDLIRQMGQKIVNGESFEPGKRSRLWLVHDVLKVFADNSGTIAVKAIKL 548

Query: 541 LIAK----DIYSEAFRNMKNLRLLILQRVAYFPRNIFEYLPNSLKWIEWSTFYVNQSSSI 600
            ++     D+ S AFRNMKNLRLLI+ R A F  N+ EYLP++LKWI+W  F        
Sbjct: 549 DLSNPTRLDVDSRAFRNMKNLRLLIV-RNARFSTNV-EYLPDNLKWIKWHGF-------- 608

Query: 601 SFSETPNFFATLNLEKLYLRGCTSLKGMRPSFRKFPSHLKFKSLKVLNLRDCLNLEEITD 660
           S    P  F   NL  L LR  + ++ +   F+        K LK ++L     LE+I D
Sbjct: 609 SHRFLPLSFLKKNLVGLDLRH-SLIRNLGKGFK------DCKRLKHVDLSYSSLLEKIPD 668

Query: 661 FSMASNLEILDLNTCFSLRIIHESIGSLDKLITLQLDLCHNLEKLPSSLKLKSLDSLSFT 720
           F   SNLE L LN C +LR I +S+ SL KL+TL LD C NL KLPS L LKSL  L   
Sbjct: 669 FPATSNLEELYLNNCTNLRTIPKSVVSLGKLLTLDLDHCSNLIKLPSYLMLKSLKVLKLA 728

Query: 721 NCYKLEQLPEFDENMKSLRVMNLNGTAIRVLPSSIGYLIGLENLNLNDCANLTALPNEIH 780
            C KLE+LP+F       ++     T +R++  SIG L  L  L+L  C+NL  LP+ + 
Sbjct: 729 YCKKLEKLPDFSTASNLEKLYLKECTNLRMIHDSIGSLSKLVTLDLGKCSNLEKLPSYL- 788

Query: 781 WLKSLEELHLRGCSKLDMFPPRSSLNFSQESSYFKLTVLDLKNCNISNSDFLETLSNVCT 840
            LKSLE L+L  C KL+  P          SS   L  L L+ C  +N   +        
Sbjct: 789 TLKSLEYLNLAHCKKLEEIP--------DFSSALNLKSLYLEQC--TNLRVIHESIGSLN 848

Query: 841 SLEKLNL-SGNKFSCLPSLQNFKSLRFLELRNCKFLQNIIKLPHHLARVNASGSELLAIR 892
           SL  L+L        LPS    KSLR  EL  C  L+   K+  ++  + +   +  AIR
Sbjct: 849 SLVTLDLRQCTNLEKLPSYLKLKSLRHFELSGCHKLEMFPKIAENMKSLISLHLDSTAIR 875

BLAST of Cucsa.275630 vs. TrEMBL
Match: A0A0A0LLK3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G020890 PE=4 SV=1)

HSP 1 Score: 272.3 bits (695), Expect = 2.2e-69
Identity = 166/343 (48.40%), Postives = 204/343 (59.48%), Query Frame = 1

Query: 598  PNFFATLNLEKLYLRGCTSLK-----------------GMRPSFRKFPSHLKFKSLKVLN 657
            P+F    NLEKLYL+ CT+L+                 G   +  K PS+L  KSL+ LN
Sbjct: 715  PDFSTASNLEKLYLKECTNLRMIHDSIGSLSKLVTLDLGKCSNLEKLPSYLTLKSLEYLN 774

Query: 658  LRDCLNLEEITDFSMASNLEILDLNTCFSLRIIHESIGSLDKLITLQLDLCHNLEKLPSS 717
            L  C  LEEI DFS A NL+ L L  C +LR+IHESIGSL+ L+TL L  C NLEKLPS 
Sbjct: 775  LAHCKKLEEIPDFSSALNLKSLYLEQCTNLRVIHESIGSLNSLVTLDLRQCTNLEKLPSY 834

Query: 718  LKLKSLDSLSFTNCYKLEQLPEFDENMKSLRVMNLNGTAIRVLPSSIGYLIGLENLNLND 777
            LKLKSL     + C+KLE  P+  ENMKSL  ++L+ TAIR LPSSIGYL  L  LNL+ 
Sbjct: 835  LKLKSLRHFELSGCHKLEMFPKIAENMKSLISLHLDSTAIRELPSSIGYLTALLVLNLHG 894

Query: 778  CANLTALPNEIHWLKSLEELHLRGCSKLDMFPPR------------------SSLNF--- 837
            C NL +LP+ I+ LKSL+ L+L GCS+  +F  +                  SS  F   
Sbjct: 895  CTNLISLPSTIYLLKSLKHLYLGGCSRFQLFSRKWDPTAHPLCSFSKIMDTSSSSEFPHL 954

Query: 838  --SQESSYFKLTVLDLKNCNISNSDFLETLSNVCTSLEKLNLSGNKFSCL-PSLQNFKSL 897
               +ES   K T+LDL+ CNISN DFLE L NV   L  + LS NKFS L P L  F SL
Sbjct: 955  LVPKESLCSKFTMLDLQCCNISNVDFLEILCNVAPFLSSILLSENKFSSLPPCLHKFMSL 1014

Query: 898  RFLELRNCKFLQNIIKLPHHLARVNASGSELLAIRPDCIADMI 900
              L+LRNCKFLQ I  LPH + +++A+G  LL   PD I D+I
Sbjct: 1015 WNLQLRNCKFLQEIPNLPHCIQKMDATGCTLLGRSPDNIMDII 1057

HSP 2 Score: 178.7 bits (452), Expect = 3.3e-41
Identity = 125/307 (40.72%), Postives = 167/307 (54.40%), Query Frame = 1

Query: 585 YVNQSSSISFSETPNFFATLNLEKLYLRGCTSLKGMRPS-----------------FRKF 644
           +V+ S S    + P+F AT NLE+LYL  CT+L+ +  S                   K 
Sbjct: 632 HVDLSYSSLLEKIPDFPATSNLEELYLNNCTNLRTIPKSVVSLGKLLTLDLDHCSNLIKL 691

Query: 645 PSHLKFKSLKVLNLRDCLNLEEITDFSMASNLEILDLNTCFSLRIIHESIGSLDKLITLQ 704
           PS+L  KSLKVL L  C  LE++ DFS ASNLE L L  C +LR+IH+SIGSL KL+TL 
Sbjct: 692 PSYLMLKSLKVLKLAYCKKLEKLPDFSTASNLEKLYLKECTNLRMIHDSIGSLSKLVTLD 751

Query: 705 LDLCHNLEKLPSSLKLKSLDSLSFTNCYKLEQLPEFDE--NMKSLRVMNLNGTAIRVLPS 764
           L  C NLEKLPS L LKSL+ L+  +C KLE++P+F    N+KSL +     T +RV+  
Sbjct: 752 LGKCSNLEKLPSYLTLKSLEYLNLAHCKKLEEIPDFSSALNLKSLYLEQC--TNLRVIHE 811

Query: 765 SIGYLIGLENLNLNDCANLTALPNEIHWLKSLEELHLRGCSKLDMFPPRSSLNFSQESSY 824
           SIG L  L  L+L  C NL  LP+ +  LKSL    L GC KL+MFP  +          
Sbjct: 812 SIGSLNSLVTLDLRQCTNLEKLPSYLK-LKSLRHFELSGCHKLEMFPKIAE--------- 871

Query: 825 FKLTVLDLKNCNISNSDFLETLSNV--CTSLEKLNLSG--NKFSCLPSLQNFKSLRFLEL 869
               +  L + ++ ++   E  S++   T+L  LNL G  N  S   ++   KSL+ L L
Sbjct: 872 ---NMKSLISLHLDSTAIRELPSSIGYLTALLVLNLHGCTNLISLPSTIYLLKSLKHLYL 923

HSP 3 Score: 74.3 bits (181), Expect = 8.8e-10
Identity = 77/266 (28.95%), Postives = 118/266 (44.36%), Query Frame = 1

Query: 164  DLIHDLVKEVLSILNQTQLLHVAKHPVGIDSQLRAVEELASHDVPDGVNMVGIHGMGGIG 223
            D+I    +EV +  +   +LH    PV  DS+++       H    GV    I GM G+ 
Sbjct: 1227 DVIKSFGQEVSAKSDCNAMLHAENFPVWNDSKMQQHMNFPLHVTSQGVTR--IRGMEGMA 1286

Query: 224  KTTLAKALYNKIAYQFEACCFLSNVRETLEQFKDLVQLQEKLLSEILKDNAWKVGNVHKG 283
            +TTLA ++ NK    +E    L + ++ L      +      LS  + D           
Sbjct: 1287 ETTLANSICNK----YERSRNLFSAKKALNHSTGFLCGDGNGLSWEMVD----------- 1346

Query: 284  KNIIRDRLCSKKVLIILDDVDKDEQLDALVGERDWFGRGSKIIATTRDRHLLENHSFDIV 343
            + I+ DRL S+K L I DD D+   L+ +       G G++     R R L         
Sbjct: 1347 RPILSDRLSSQKYLRIFDDRDRYGDLNDVA-----HGTGNRF----RSRFLR-------- 1406

Query: 344  YPIQLLDPKKSLELFSLHAFKQNHPSSNYVDLSKFAVSYCKGLPLALVILGSLLHKRERK 403
                 +D  K  ++     +K  +   +Y+DLSK AV YCKGLPL L +L S L+ R++ 
Sbjct: 1407 -----MDDIKEDDIREEPYWK--YMERDYLDLSKQAVDYCKGLPLGLEVLDSFLYNRDQS 1451

Query: 404  IWKSKLHELE-NSLEPSVEAVFQIGF 429
              KS L   E +SL   ++ + QI F
Sbjct: 1467 KCKSVLDSYEKSSLHKRIQEILQIKF 1451

HSP 4 Score: 776.2 bits (2003), Expect = 4.7e-221
Identity = 460/907 (50.72%), Postives = 595/907 (65.60%), Query Frame = 1

Query: 11  KWSYDVFLSFRGEDTRNNFTSHLDRALREKGVNFFIDDKLERGGQISESLLKSIDGSKIS 70
           KW+YDVFLS+RGEDTR NFTSHLD ALR+KGVN FIDDKLERG QISE+LLKSI  + IS
Sbjct: 14  KWNYDVFLSYRGEDTRTNFTSHLDMALRQKGVNVFIDDKLERGKQISETLLKSIQEALIS 73

Query: 71  IIIFSKNYASSTWCLDELVKIVQCMKSMGHIVFPVFYKVDPSEVRKQTGGFGEALAKHEA 130
           IIIFS+NYASS+WCLDELV I++C KS   IV PVFYKVDPS++RKQ+G FGEALAKH+A
Sbjct: 74  IIIFSQNYASSSWCLDELVNIIECKKSKDQIVLPVFYKVDPSDIRKQSGSFGEALAKHQA 133

Query: 131 NELMTNKVQPWKEALTTAASLSGWDLATRKNEADLIHDLVKEVLSILNQTQL-LHVAKHP 190
                 K+Q W+EALTTAA+LSGWDL TRK EADLI D+VK+VLS LN+T + L+VAK+P
Sbjct: 134 K--FKTKIQIWREALTTAANLSGWDLGTRK-EADLIGDIVKKVLSTLNRTCMPLYVAKYP 193

Query: 191 VGIDSQLRAVEELASHDVPD-----------------GVNMVGIHGMGGIGKTTLAKALY 250
           VGIDS+L  ++ L SH++ +                 G+ MVGI+G+GGIGKTTLAKALY
Sbjct: 194 VGIDSKLEYIK-LRSHNMFEKNNKFHYRTQHEYEFDTGIYMVGIYGIGGIGKTTLAKALY 253

Query: 251 NKIAYQFEACCFLSNVRETLEQFKDLVQLQEKLLSEILKDNAWKVGNVHKGKNIIRDRLC 310
           NKIA QFE CCFLSNVRE  +QF  L QLQE LL EIL  +  KV N+ +G NIIR+RLC
Sbjct: 254 NKIASQFEGCCFLSNVREASKQFNGLAQLQESLLYEILMVDL-KVVNLDRGINIIRNRLC 313

Query: 311 SKKVLIILDDVDKDEQLDALVGERDWFGRGSKIIATTRDRHLLENHSFDIVYPIQLLDPK 370
           SKKVLI+LDDVDK EQL+ALVG  DWFG+GS+II TTR++HLL +H FD ++ I  L+  
Sbjct: 314 SKKVLIVLDDVDKLEQLEALVGGCDWFGKGSRIIVTTRNKHLLFSHGFDEIHNILGLNED 373

Query: 371 KSLELFSLHAFKQNHPSSNYVDLSKFAVSYCKGLPLALVILGSLLHKRERKIWKSKLHEL 430
           K++ELFS HAFK+N PSSNY+DLSK A SYCKG PLALV+LGS L  R++  W S L E 
Sbjct: 374 KAIELFSWHAFKKNRPSSNYLDLSKRATSYCKGHPLALVVLGSFLCIRDQAEWCSILDEF 433

Query: 431 ENSLEPSVEAVFQIGFKELHERVKEIFLDISCFFVGEDINYSKDVLKACDLNPDYGIIIL 490
           ENSL   ++ + Q+ F  L +++K+IFLDISC  VGE + Y KD+L AC +N D+G+I+L
Sbjct: 434 ENSLNKDIKDILQLSFDGLEDKIKDIFLDISCLLVGEKVEYVKDMLGACHVNLDFGVIVL 493

Query: 491 MDLSLVTVEDGKIQMHDLIQQMGQTIVRHESFEPAKRSRLWEAEGAIKILKEKSVSDFRQ 550
           MDLSL+T+E+ K+QMHDLI+QMGQ IV  ES E  KRSRLW  +   ++L   S +D  +
Sbjct: 494 MDLSLITIENDKVQMHDLIKQMGQKIVCGESLELGKRSRLWLVQDVWEVLVNNSGTDAIK 553

Query: 551 CFYLLIAK----DIYSEAFRNMKNLRLLILQRVAYFPRNIFEYLPNSLKWIEWSTFYVNQ 610
              L         + S+AFR MKNLRLLI+Q   +  +   EYLP+SLKWI+W  F    
Sbjct: 554 AIKLDFPNPTRLGVNSQAFRKMKNLRLLIVQNARFSTK--IEYLPDSLKWIKWHGF---- 613

Query: 611 SSSISFSETPNFFATLNLEKLYLRGCTSLKGMRPSFRK-FPSHLK-FKSLKVLNLRDCLN 670
                    P+ F T NL  L          ++ SF K F   L+  K LK ++L     
Sbjct: 614 ----PQPTLPSCFITKNLVGL---------DLQYSFMKTFGKRLEDCKRLKHVDLSHSTF 673

Query: 671 LEEITDFSMASNLEILDLNTCFSLRIIHESIGSLDKLITLQLDLCHNLEKLPSS-LKLKS 730
           LE+I +FS ASNLE L L  C +L +I +S+ SLDKL  L L  C NL+KLP     L+S
Sbjct: 674 LEKIPNFSAASNLEELYLINCKNLGMIDKSVFSLDKLTILNLAGCSNLKKLPRGYFILRS 733

Query: 731 LDSLSFTNCYKLEQLPEFDENMKSLRVMNLNGTAIRVLPSSIGYLIGLENLNLNDCANLT 790
           L  L+ ++C KLE++P+F        +   N T +R++  S+  L  L  LNL+ C+NL 
Sbjct: 734 LRYLNLSHCKKLEKIPDFSAASNLEELYLFNCTNLRMIDKSVFSLHKLTILNLDVCSNLK 793

Query: 791 ALPNEIHWLKSLEELHLRGCSKLDMFPPRSSLNFSQESSYFKLTVLDLKNCNI-SNSDFL 850
            LP   + L SL+ L+L  C KL+  P  S+ +  Q     + T L L + ++ S    +
Sbjct: 794 KLPTSYYKLWSLQYLNLSYCKKLEKIPDLSAASNLQSLCLHECTNLRLIHESVGSLYKLI 853

Query: 851 ETLSNVCTSLEKLNLSGNKFSCLPSLQNFKSLRFLELRNCKFLQNIIKLPHHLARVNASG 892
           +   + CT+L K          LP+    KSLR+L L  C  L++   +  ++  +    
Sbjct: 854 DMDLSGCTNLAK----------LPTYLRLKSLRYLGLSECCKLESFPSIAENMESLRELD 886

BLAST of Cucsa.275630 vs. TrEMBL
Match: E5GB33_CUCME (TIR-NBS-LRR disease resistance protein OS=Cucumis melo subsp. melo PE=4 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 7.3e-65
Identity = 161/357 (45.10%), Postives = 211/357 (59.10%), Query Frame = 1

Query: 585  YVNQSSSISFSETPNFFATLNLEKLYLRGCTSLKGMRPS-----------------FRKF 644
            Y+N S      + P+F A  NLE+LYL  CT+L+ +  S                  +K 
Sbjct: 712  YLNLSHCKKLEKIPDFSAASNLEELYLFNCTNLRMIDKSVFSLHKLTILNLDVCSNLKKL 771

Query: 645  P-SHLKFKSLKVLNLRDCLNLEEITDFSMASNLEILDLNTCFSLRIIHESIGSLDKLITL 704
            P S+ K  SL+ LNL  C  LE+I D S ASNL+ L L+ C +LR+IHES+GSL KLI +
Sbjct: 772  PTSYYKLWSLQYLNLSYCKKLEKIPDLSAASNLQSLCLHECTNLRLIHESVGSLYKLIDM 831

Query: 705  QLDLCHNLEKLPSSLKLKSLDSLSFTNCYKLEQLPEFDENMKSLRVMNLNGTAIRVLPSS 764
             L  C NL KLP+ L+LKSL  L  + C KLE  P   ENM+SLR ++++ TAI+ LPSS
Sbjct: 832  DLSGCTNLAKLPTYLRLKSLRYLGLSECCKLESFPSIAENMESLRELDMDFTAIKELPSS 891

Query: 765  IGYLIGLENLNLNDCANLTALPNEIHWLKSLEELHLRGCSKLDMFP--------PRSSLN 824
            IGYL  L  LNL  C NL +LPN I+ L++L++L L GCS+ +MFP        P  S +
Sbjct: 892  IGYLTQLYRLNLTGCTNLISLPNTIYLLRNLDKLLLSGCSRFEMFPHKWDPTIQPVCSPS 951

Query: 825  FSQESSYFKL---------------TVLDLKNCNISNSDFLETLSNVCTSLEKLNLSGNK 884
               E++ + L               T+LDL++CNISN+ FLE L +V   L  L LS NK
Sbjct: 952  KMMEATSWSLEYPHLLPNESLCSHFTLLDLQSCNISNAKFLEILCDVAPFLSDLRLSENK 1011

Query: 885  FSCLPS-LQNFKSLRFLELRNCKFLQNIIKLPHHLARVNASGSELLAIRPDCIADMI 900
            FS LPS L  F SL  LEL+NCKFLQ I  LP ++  ++ASG + LA  PD I D+I
Sbjct: 1012 FSSLPSCLHKFMSLWNLELKNCKFLQEIPNLPQNIQNLDASGCKSLARSPDNIMDII 1068

HSP 2 Score: 136.7 bits (343), Expect = 1.4e-28
Identity = 112/303 (36.96%), Postives = 158/303 (52.15%), Query Frame = 1

Query: 585 YVNQSSSISFSETPNFFATLNLEKLYLRGCTSLKGMRPS-----------------FRKF 644
           +V+ S S    + PNF A  NLE+LYL  C +L  +  S                  +K 
Sbjct: 641 HVDLSHSTFLEKIPNFSAASNLEELYLINCKNLGMIDKSVFSLDKLTILNLAGCSNLKKL 700

Query: 645 P-SHLKFKSLKVLNLRDCLNLEEITDFSMASNLEILDLNTCFSLRIIHESIGSLDKLITL 704
           P  +   +SL+ LNL  C  LE+I DFS ASNLE L L  C +LR+I +S+ SL KL  L
Sbjct: 701 PRGYFILRSLRYLNLSHCKKLEKIPDFSAASNLEELYLFNCTNLRMIDKSVFSLHKLTIL 760

Query: 705 QLDLCHNLEKLPSS-LKLKSLDSLSFTNCYKLEQLPEFD--ENMKSLRVMNLNGTAIRVL 764
            LD+C NL+KLP+S  KL SL  L+ + C KLE++P+     N++SL +     T +R++
Sbjct: 761 NLDVCSNLKKLPTSYYKLWSLQYLNLSYCKKLEKIPDLSAASNLQSLCLHEC--TNLRLI 820

Query: 765 PSSIGYLIGLENLNLNDCANLTALPNEIHWLKSLEELHLRGCSKLDMFPPRSSLNFSQES 824
             S+G L  L +++L+ C NL  LP  +  LKSL  L L  C KL+ FP   S+  + ES
Sbjct: 821 HESVGSLYKLIDMDLSGCTNLAKLPTYLR-LKSLRYLGLSECCKLESFP---SIAENMES 880

Query: 825 SYFKLTVLDLKNCNISNSDFLETLSNVCTSLEKLNLSG--NKFSCLPSLQNFKSLRFLEL 865
               L  LD+    I     L +     T L +LNL+G  N  S   ++   ++L  L L
Sbjct: 881 ----LRELDMDFTAIKE---LPSSIGYLTQLYRLNLTGCTNLISLPNTIYLLRNLDKLLL 930

HSP 3 Score: 758.1 bits (1956), Expect = 1.3e-215
Identity = 477/955 (49.95%), Postives = 591/955 (61.88%), Query Frame = 1

Query: 7   SSNLKWSYDVFLSFRGEDTRNNFTSHLDRALREKGVNFFIDDKLERGGQISESLLKSIDG 66
           S   KW+YDVFLSFRGEDTR NFTSHLD ALR+KGVN FID+KLERG QISESL KSI  
Sbjct: 40  SPTFKWTYDVFLSFRGEDTRTNFTSHLDMALRQKGVNVFIDNKLERGEQISESLFKSIQE 99

Query: 67  SKISIIIFSKNYASSTWCLDELVKIVQCMKSMGHIVFPVFYKVDPSEVRKQTGGFGEALA 126
           + ISI+IFS+NYASS+WCLDELV I++C KS G  VFPVFYKVDPS++RKQTG FGEALA
Sbjct: 100 ASISIVIFSQNYASSSWCLDELVNIIECKKSKGQNVFPVFYKVDPSDIRKQTGSFGEALA 159

Query: 127 KHEANELMTNKVQPWKEALTTAASLSGWDLATRKNEADLIHDLVKEVLSILNQT-QLLHV 186
           KH+       K Q W+EALTTAA+LSGW+L TRK EADLI DLVK+VLS+LN+T   L+V
Sbjct: 160 KHQPK--FQTKTQIWREALTTAANLSGWNLGTRK-EADLIGDLVKKVLSVLNRTCTPLYV 219

Query: 187 AKHPVGIDSQLRAVEELASHDV-----------------PDGVNMVGIHGMGGIGKTTLA 246
           AK+PVGIDS+L  + +L SH++                   GV MVG++G+GGIGKTTLA
Sbjct: 220 AKYPVGIDSKLEYM-KLRSHNLFEKSNKFHYRKQHEYESDTGVYMVGLYGIGGIGKTTLA 279

Query: 247 KALYNKIAYQFEACCFLSNVRETLEQFKDLVQLQEKLLSEILKDNAWKVGNVHKGKNIIR 306
           KALYNKIA QFEACCFLSNVRE  +QF  L QLQE LL EIL  +  KV N+ +G NIIR
Sbjct: 280 KALYNKIASQFEACCFLSNVREASKQFNGLAQLQETLLYEILTVDL-KVINLDRGINIIR 339

Query: 307 DRLCSKKVLIILDDVDKDEQLDALVGERDWFGRGSKIIATTRDRHLLENHSFDIVYPIQL 366
           +RLC KKVLI+LDDVDK EQL+ALVG RDWFG+GS+II TTR++HLL +H FD +  I  
Sbjct: 340 NRLCLKKVLIVLDDVDKLEQLEALVGGRDWFGQGSRIIVTTRNKHLLSSHGFDEMENILG 399

Query: 367 LDPKKSLELFSLHAFKQNHPSSNYVDLSKFAVSYCKGLPLALVILGSLLHKRERKIWKSK 426
           LD  +++ELFS HAFK+NHPSSNY+DLSK A SYCKG  LALV+LGS L  R++  W S 
Sbjct: 400 LDEDEAIELFSWHAFKKNHPSSNYLDLSKRATSYCKGHSLALVVLGSFLCTRDQVEWCSI 459

Query: 427 LHELENSLEPSVEAVFQIGFKELHERVKEIFLDISCFFVGEDINYSKDVLKACDLNPDYG 486
           L E ENSL   ++ + Q+ F  L +++      I C   GE +   K       L  D  
Sbjct: 460 LDEFENSLNKDIKDILQLSFDGLEDKMGH---KIVC---GESLELGK--RSRLWLVQDVW 519

Query: 487 IIILMDLSLVTVEDGKIQMHDLIQQMGQTIVRHESFEPAKRSRLWEAEGAIKILKEKSVS 546
            +++ +     V+  K+          +  V  ++F   K  RL   + A    K + + 
Sbjct: 520 EVLVNNSGTDAVKGIKLD----FPNSTRLDVDPQAFRKMKNLRLLIVQNARFSTKIEYLP 579

Query: 547 DFRQCFYLLIAKDIYSEAFRNMKNLRLLILQR--VAYFPRNIFEYLPNSLKWIEWSTFYV 606
           D  +       +     +F  MKNL  L LQ   +  F + + +     LK       YV
Sbjct: 580 DSLKWIKWHGFRQPTFPSFFTMKNLVGLDLQHSFIKTFGKRLEDC--ERLK-------YV 639

Query: 607 NQSSSISFSETPNFFATLNLEKLYLRGCTSLKGMRPS-----------------FRKFP- 666
           + S S    + PNF A  NLE+LYL  CT+L  +  S                  +K P 
Sbjct: 640 DLSYSTFLEKIPNFSAASNLEELYLTNCTNLGMIDKSVFSLDKLTVLNLDGCSNLKKLPR 699

Query: 667 SHLKFKSLKVLNLRDCLNLEEITDFSMASNLEILDLNTCFSLRIIHESIGSLDKLITLQL 726
            +    SLK LNL  C  LE+I D S ASNL  L +  C +LR+IHES+GSLDKL  L L
Sbjct: 700 GYFMLSSLKKLNLSYCKKLEKIPDLSSASNLTSLHIYECTNLRVIHESVGSLDKLEGLYL 759

Query: 727 DLCHNLEKLPSSLKLKSLDSLSFTNCYKLEQLPEFDENMKSLRVMNLNGTAIRVLPSSIG 786
             C NL KLPS L LKSL  LS + C KLE  P   +NMKSLR ++L+ TAI+ LPSSI 
Sbjct: 760 KQCTNLVKLPSYLSLKSLLCLSLSGCCKLESFPTIAKNMKSLRTLDLDFTAIKELPSSIR 819

Query: 787 YLIGLENLNLNDCANLTALPNEIHWLKSLEELHLRGCSKLDMFP--------PRSSLNFS 846
           YL  L  L LN C NL +LPN I+ L+SLE L L GCS   MFP        P  S +  
Sbjct: 820 YLTELWTLKLNGCTNLISLPNTIYLLRSLENLLLSGCSIFGMFPDKWNPTIQPVCSPSKM 879

Query: 847 QESSYFKL---------------TVLDLKNCNISNSDFLETLSNVCTSLEKLNLSGNKFS 900
            E++ + L               T+LDL++CNISN++FL+ L +V   L  L LS NKFS
Sbjct: 880 METALWSLKVPHFLVPNESFSHFTLLDLQSCNISNANFLDILCDVAPFLSDLRLSENKFS 939

BLAST of Cucsa.275630 vs. TAIR10
Match: AT5G36930.2 (AT5G36930.2 Disease resistance protein (TIR-NBS-LRR class) family)

HSP 1 Score: 524.6 bits (1350), Expect = 1.2e-148
Identity = 338/895 (37.77%), Postives = 512/895 (57.21%), Query Frame = 1

Query: 11  KWSYDVFLSFRGEDTRNNFTSHLDRALREKGVNFFIDD-KLERGGQISESLLKSIDGSKI 70
           +W+YDVF+SFRG D R NF SHL  +LR  G++ F+DD +L+RG  IS  LL +I+ SKI
Sbjct: 14  RWTYDVFVSFRGADVRKNFLSHLYDSLRRCGISTFMDDVELQRGEYISPELLNAIETSKI 73

Query: 71  SIIIFSKNYASSTWCLDELVKIVQCMKSM-GHIVFPVFYKVDPSEVRKQTGGFGEALAKH 130
            I++ +K+YASS WCLDELV I++  K+   H+VFP+F  VDPS++R Q G + ++ +KH
Sbjct: 74  LIVVLTKDYASSAWCLDELVHIMKSHKNNPSHMVFPIFLYVDPSDIRWQQGSYAKSFSKH 133

Query: 131 EANELMTNKVQPWKEALTTAASLSGWDLATRKNEADLIHDLVKEVLSILNQTQLLHVAKH 190
           + N    NK++ W+EALT  A++SGWD+  R NEA+ I D+ +E+L  L   Q LHV  +
Sbjct: 134 K-NSHPLNKLKDWREALTKVANISGWDIKNR-NEAECIADITREILKRL-PCQYLHVPSY 193

Query: 191 PVGIDSQLRAVEELASHDVPDGVNMVGIHGMGGIGKTTLAKALYNKIAYQFEACCFLSNV 250
            VG+ S+L+ +  L S    DGV ++ I+GMGGIGKTTLAK  +N+ ++ FE   FL N 
Sbjct: 194 AVGLRSRLQHISSLLSIG-SDGVRVIVIYGMGGIGKTTLAKVAFNEFSHLFEGSSFLENF 253

Query: 251 RETLEQFKDLVQLQEKLLSEILKDNAWKVGNVHKGKNIIRDRLCSKKVLIILDDVDKDEQ 310
           RE  ++ +    LQ +LLS+IL+ N  +   +    + +++R  SK+VL+++DDVD   Q
Sbjct: 254 REYSKKPEGRTHLQHQLLSDILRRNDIEFKGLD---HAVKERFRSKRVLLVVDDVDDVHQ 313

Query: 311 LDALVGERDWFGRGSKIIATTRDRHLLENHSFDIVYPIQLLDPKKSLELFSLHAFKQNHP 370
           L++   +RD FG GS+II TTR+ HLL+    +  Y  + LD  +SLELFS HAF+ + P
Sbjct: 314 LNSAAIDRDCFGHGSRIIITTRNMHLLKQLRAEGSYSPKELDGDESLELFSWHAFRTSEP 373

Query: 371 SSNYVDLSKFAVSYCKGLPLALVILGSLLHKRERKIWKSKLHELENSLEPSVEAVFQIGF 430
              ++  S+  V+YC GLPLA+ +LG+ L +R  + W+S L  L+     +++A  QI F
Sbjct: 374 PKEFLQHSEEVVTYCAGLPLAVEVLGAFLIERSIREWESTLKLLKRIPNDNIQAKLQISF 433

Query: 431 KELHERVKEIFLDISCFFVGEDINYSKDVLKACDLNPDYGIIILMDLSLVTVEDGKIQMH 490
             L    K++FLDI+CFF+G D  Y   +L  C+L PD  + +LM+  L+T+    I MH
Sbjct: 434 NALTIEQKDVFLDIACFFIGVDSYYVACILDGCNLYPDIVLSLLMERCLITISGNNIMMH 493

Query: 491 DLIQQMGQTIVRHESFEP-AKRSRLWEAEGAIKILKEKSVSDFRQCFYLLIAKDIYS--- 550
           DL++ MG+ IVR  S +   +RSRLW     + +LK+KS ++  +   L +  D+     
Sbjct: 494 DLLRDMGRQIVREISPKKCGERSRLWSHNDVVGVLKKKSGTNAIE--GLSLKADVMDFQY 553

Query: 551 ---EAFRNMKNLRLLILQRVAYFPRNIFEYLPNSLKWIEWSTFYVNQSSSISFSETPNFF 610
              EAF  M+ LRLL L+ V       +E+ P  L+W+ W  F        S    P   
Sbjct: 554 FEVEAFAKMQELRLLELRYVDL--NGSYEHFPKDLRWLCWHGF--------SLECFPINL 613

Query: 611 ATLNLEKLYLRGCTSLKGMRPSFRKFPSHLKFKSLKVLNLRDCLNLEEITDFSMASNLEI 670
           +  +L  L L+     +  +      P+++    +K L+L   + L E  DFS   N+E 
Sbjct: 614 SLESLAALDLQYSNLKRFWKAQSPPQPANM----VKYLDLSHSVYLRETPDFSYFPNVEK 673

Query: 671 LDLNTCFSLRIIHESIGSLD-KLITLQLDLCHNLEKLPSSL-KLKSLDSLSFTNCYKLEQ 730
           L L  C SL ++H+SIG LD KL+ L L  C  L+ LP  + KLKSL+SL  +NC KLE+
Sbjct: 674 LILINCKSLVLVHKSIGILDKKLVLLNLSSCIELDVLPEEIYKLKSLESLFLSNCSKLER 733

Query: 731 LPEFDENMKSLRVMNLNGTAIRVLPSSIGYLIGLENLNLNDCANLTALPNEIHWLKSLEE 790
           L +    ++SL  +  + TA+R +PS+I  L  L+ L+LN C  L +          ++ 
Sbjct: 734 LDDALGELESLTTLLADFTALREIPSTINQLKKLKRLSLNGCKGLLS--------DDIDN 793

Query: 791 LHLRGCSKLDMFPPRSSLNFSQESSYFKLTVLDLKNCNISNSDFLETLSNVCTSLEKLNL 850
           L+      + +  P S       S    + +L L  CN+S+    E + ++ + L  L+L
Sbjct: 794 LYSEKSHSVSLLRPVSL------SGLTYMRILSLGYCNLSDELIPEDIGSL-SFLRDLDL 853

Query: 851 SGNKFSCLPS-LQNFKSLRFLELRNCKFLQNIIKLPHHLARVNASGSELLAIRPD 894
            GN F  LP+      +L  L L +C  LQ+I+ LP  L  ++     +L   PD
Sbjct: 854 RGNSFCNLPTDFATLPNLGELLLSDCSKLQSILSLPRSLLFLDVGKCIMLKRTPD 870

HSP 2 Score: 85.5 bits (210), Expect = 1.9e-16
Identity = 98/358 (27.37%), Postives = 150/358 (41.90%), Query Frame = 1

Query: 585 YVNQSSSISFSETPNFFATLNLEKLYLRGCTSLKGMRPSFRKFPSHLKFKSLKVLNLRDC 644
           Y++ S S+   ETP+F    N+EKL L  C SL  +  S       +  K L +LNL  C
Sbjct: 627 YLDLSHSVYLRETPDFSYFPNVEKLILINCKSLVLVHKSI-----GILDKKLVLLNLSSC 686

Query: 645 LNLEEI-TDFSMASNLEILDLNTCFSLRIIHESIGSLDKLITLQLDLCHNLEKLPSSL-K 704
           + L+ +  +     +LE L L+ C  L  + +++G L+ L TL  D    L ++PS++ +
Sbjct: 687 IELDVLPEEIYKLKSLESLFLSNCSKLERLDDALGELESLTTLLADFT-ALREIPSTINQ 746

Query: 705 LKSLDSLSFTNCYKL------EQLPEFDENMKSLRVMNLNG-TAIRVL------------ 764
           LK L  LS   C  L          E   ++  LR ++L+G T +R+L            
Sbjct: 747 LKKLKRLSLNGCKGLLSDDIDNLYSEKSHSVSLLRPVSLSGLTYMRILSLGYCNLSDELI 806

Query: 765 PSSIGYLIGLENLNLNDCANLTALPNEIHWLKSLEELHLRGCSKLD--MFPPRSSLNFSQ 824
           P  IG L  L +L+L    +   LP +   L +L EL L  CSKL   +  PRS L    
Sbjct: 807 PEDIGSLSFLRDLDLRG-NSFCNLPTDFATLPNLGELLLSDCSKLQSILSLPRSLLFLDV 866

Query: 825 ESSYFKLTVLDLKNCNISNSDFLETLSNVCTSLEKLNLSGNKFSCLPSLQNFKSLRFLEL 884
                     D+  C    S   +   N C SL +          +P + N + L F+ L
Sbjct: 867 GKCIMLKRTPDISKC----SALFKLQLNDCISLFE----------IPGIHNHEYLSFIVL 926

Query: 885 RNCKFLQNIIKLPHHLARVNASGSELLAI---RPDCIADMIYLHANDRHHIKVLFPNT 917
             CK       +   L        E + I   RP+ I + +Y    ++    +  P T
Sbjct: 927 DGCKLASTDTTINTMLENWLKRNHECIYIPVDRPNVIPNWVYFE-EEKRSFSITVPET 962

BLAST of Cucsa.275630 vs. TAIR10
Match: AT5G17680.1 (AT5G17680.1 disease resistance protein (TIR-NBS-LRR class), putative)

HSP 1 Score: 489.2 bits (1258), Expect = 5.8e-138
Identity = 324/913 (35.49%), Postives = 501/913 (54.87%), Query Frame = 1

Query: 2   SSSSCSSNLKWSYDVFLSFRGEDTRNNFTSHLDRALREKGVNFFIDD-KLERGGQISESL 61
           SSSS SS+  W  DVF+SFRGED R  F SHL       G+  F DD  L+RG  IS  L
Sbjct: 6   SSSSSSSSTVWKTDVFVSFRGEDVRKTFVSHLFCEFDRMGIKAFRDDLDLQRGKSISPEL 65

Query: 62  LKSIDGSKISIIIFSKNYASSTWCLDELVKIVQCMKSMGHIVFPVFYKVDPSEVRKQTGG 121
           + +I GS+ +I++ S+NYA+S+WCLDEL+KI++C K     + P+FY+VDPS+VR+Q G 
Sbjct: 66  IDAIKGSRFAIVVVSRNYAASSWCLDELLKIMECNKDT---IVPIFYEVDPSDVRRQRGS 125

Query: 122 FGEALAKHEANELMTNKVQPWKEALTTAASLSGWDLATRKNEADLIHDLVKEVLSILNQT 181
           FGE +  H   E    KV  WKEAL   A++SG D +   +++ LI  +VK++   L  T
Sbjct: 126 FGEDVESHSDKE----KVGKWKEALKKLAAISGED-SRNWDDSKLIKKIVKDISDKLVST 185

Query: 182 QLLHVAKHPVGIDSQLRAVEELASHDVPDGVNMVGIHGMGGIGKTTLAKALYNKIAYQFE 241
                +K  +G+ S +  ++ + S  V   V M+GI GMGG+GKTT+AK LYN+++ QF+
Sbjct: 186 SW-DDSKGLIGMSSHMDFLQSMISI-VDKDVRMLGIWGMGGVGKTTIAKYLYNQLSGQFQ 245

Query: 242 ACCFLSNVRETLEQFKDLVQLQEKLLSEILKDNAWKVGNVHKGKNIIRDRLCSKKVLIIL 301
             CF+ NV+E   ++  + +LQ + L  + ++   +  +     NII++R   K V I+L
Sbjct: 246 VHCFMENVKEVCNRY-GVRRLQVEFLCRMFQERDKEAWSSVSCCNIIKERFRHKMVFIVL 305

Query: 302 DDVDKDEQLDALVGERDWFGRGSKIIATTRDRHLLENHSFDIVYPIQLLDPKKSLELFSL 361
           DDVD+ EQL+ LV E  WFG GS+II TTRDRHLL +H  ++VY ++ L  K++L+LF  
Sbjct: 306 DDVDRSEQLNELVKETGWFGPGSRIIVTTRDRHLLLSHGINLVYKVKCLPKKEALQLFCN 365

Query: 362 HAFKQNHPSSN-YVDLSKFAVSYCKGLPLALVILGSLLHKRERKIWKSKLHELENSLEPS 421
           +AF++     + + +LS  AV+Y  GLPLAL +LGS L++R +  W+S L  L+      
Sbjct: 366 YAFREEIILPHGFEELSVQAVNYASGLPLALRVLGSFLYRRSQIEWESTLARLKTYPHSD 425

Query: 422 VEAVFQIGFKELHERVKEIFLDISCFFVGEDINYSKDVLKACDLNPDYGIIILMDLSLVT 481
           +  V ++ +  L E+ K IFL ISCF+  + ++Y + +L  C    + GI IL + SL+ 
Sbjct: 426 IMEVLRVSYDGLDEQEKAIFLYISCFYNMKQVDYVRKLLDLCGYAAEIGITILTEKSLIV 485

Query: 482 VEDGKIQMHDLIQQMGQTIVRHESF-EPAKRSRLWEAEGAIKILKEKSVSDFRQCFYLLI 541
             +G +++HDL++QMG+ +VR ++   PA+R  LW+ E    +L E S +   +   L +
Sbjct: 486 ESNGCVKIHDLLEQMGRELVRQQAVNNPAQRLLLWDPEDICHLLSENSGTQLVEGISLNL 545

Query: 542 AK--DIYS--EAFRNMKNLRLLILQRVAYFPR------NIFEYLPNSLKWIEWSTFYVNQ 601
           ++  ++++   AF  + NL+LL    +++         N   YLP  L+++ W  + +  
Sbjct: 546 SEISEVFASDRAFEGLSNLKLLNFYDLSFDGETRVHLPNGLSYLPRKLRYLRWDGYPLKT 605

Query: 602 SSSISFSETPNFFATL-----NLEKLYLRGCTSLKGMRPSFRKFPSHLKFKSLKVLNLRD 661
             S  F   P F   L     NLEKL+        G++P           ++LK ++L  
Sbjct: 606 MPSRFF---PEFLVELCMSNSNLEKLW-------DGIQP----------LRNLKKMDLSR 665

Query: 662 CLNLEEITDFSMASNLEILDLNTCFSLRIIHESIGSLDKLITLQLDLCHNLEKLPSSLKL 721
           C  L E+ D S A+NLE L+L+ C SL  +  SI +L  L    L  C  L+ +P  + L
Sbjct: 666 CKYLVEVPDLSKATNLEELNLSYCQSLVEVTPSIKNLKGLSCFYLTNCIQLKDIPIGIIL 725

Query: 722 KSLDSLSFTNCYKLEQLPEFDENMKSLRVMNLNGTAIRVLPSSIGYLIGLENLNLNDCAN 781
           KSL+++  + C  L+  PE   N + L    L+ T I  LPSSI  L  L  L+++DC  
Sbjct: 726 KSLETVGMSGCSSLKHFPEISWNTRRL---YLSSTKIEELPSSISRLSCLVKLDMSDCQR 785

Query: 782 LTALPNEIHWLKSLEELHLRGCSKLDMFPPRSSLNFSQESSYFKLTVLDLKNCNISNSDF 841
           L  LP+ +  L SL+ L+L GC +L+  P           +   L  L++  C   N   
Sbjct: 786 LRTLPSYLGHLVSLKSLNLDGCRRLENLP-------DTLQNLTSLETLEVSGCLNVNE-- 845

Query: 842 LETLSNVCTSLEKLNLSGNKFSCLPS-LQNFKSLRFLELRNCKFLQNI---IKLPHHLAR 893
                 V TS+E L +S      +P+ + N   LR L++   K L ++   I     L +
Sbjct: 846 ---FPRVSTSIEVLRISETSIEEIPARICNLSQLRSLDISENKRLASLPVSISELRSLEK 872

HSP 2 Score: 105.9 bits (263), Expect = 1.4e-22
Identity = 111/396 (28.03%), Postives = 173/396 (43.69%), Query Frame = 1

Query: 511 RLWEAEGAIKILKEKSVSDFRQCFYLLIAKDIYSEAFRNMKNLRLLILQRVAYFPRNIFE 570
           +LW+    ++ LK+  +S   +C YL+   D+      N++ L L   Q       ++ E
Sbjct: 616 KLWDGIQPLRNLKKMDLS---RCKYLVEVPDLSKAT--NLEELNLSYCQ-------SLVE 675

Query: 571 YLPNSLKWIEWSTFYVNQSSSISFSETPNFFATLNLEKLYLRGCTSLKGMRPSFRKFPSH 630
             P+       S FY+  ++ I   + P      +LE + + GC+SLK   P        
Sbjct: 676 VTPSIKNLKGLSCFYL--TNCIQLKDIPIGIILKSLETVGMSGCSSLKHF-PEISWNTRR 735

Query: 631 LKFKSLKVLNLRDCLNLEEITDFSMASNLEILDLNTCFSLRIIHESIGSLDKLITLQLDL 690
           L   S K+  L   +        S  S L  LD++ C  LR +   +G L  L +L LD 
Sbjct: 736 LYLSSTKIEELPSSI--------SRLSCLVKLDMSDCQRLRTLPSYLGHLVSLKSLNLDG 795

Query: 691 CHNLEKLPSSLK-LKSLDSLSFTNCYKLEQLPEFDENMKSLRVMNLNGTAIRVLPSSIGY 750
           C  LE LP +L+ L SL++L  + C  + + P       S+ V+ ++ T+I  +P+ I  
Sbjct: 796 CRRLENLPDTLQNLTSLETLEVSGCLNVNEFPRVST---SIEVLRISETSIEEIPARICN 855

Query: 751 LIGLENLNLNDCANLTALPNEIHWLKSLEELHLRGCSKLDMFPPRSSLNFSQESS---YF 810
           L  L +L++++   L +LP  I  L+SLE+L L GCS L+ FP    L   Q  S   +F
Sbjct: 856 LSQLRSLDISENKRLASLPVSISELRSLEKLKLSGCSVLESFP----LEICQTMSCLRWF 915

Query: 811 KLTVLDLKNC--NISNSDFLETLSNVCTSLEKLNLS------------GNKF-------- 870
            L    +K    NI N   LE L    T + +   S            GN F        
Sbjct: 916 DLDRTSIKELPENIGNLVALEVLQASRTVIRRAPWSIARLTRLQVLAIGNSFFTPEGLLH 975

Query: 871 SCLPSLQNFKSLRFLELRNCKFLQNIIKLPHHLARV 881
           S  P L  F  LR L L N     N+ ++P+ +  +
Sbjct: 976 SLCPPLSRFDDLRALSLSN----MNMTEIPNSIGNL 977

HSP 3 Score: 87.0 bits (214), Expect = 6.6e-17
Identity = 118/400 (29.50%), Postives = 176/400 (44.00%), Query Frame = 1

Query: 512  LWEAEGAIKILKEKSVSDFRQCFYL---LIAKDI-YSEAFRNMKNLRLLILQRVAYFPRN 571
            L E   +IK LK  S      CFYL   +  KDI      ++++ + +     + +FP  
Sbjct: 661  LVEVTPSIKNLKGLS------CFYLTNCIQLKDIPIGIILKSLETVGMSGCSSLKHFPE- 720

Query: 572  IFEYLPNSLKWIEWSTFYVNQSSSISFSETPNFFATLN-LEKLYLRGCTSLKGMRPSFRK 631
                       I W+T  +  SS+    E P+  + L+ L KL +  C  L       R 
Sbjct: 721  -----------ISWNTRRLYLSST-KIEELPSSISRLSCLVKLDMSDCQRL-------RT 780

Query: 632  FPSHL-KFKSLKVLNLRDCLNLEEITD-FSMASNLEILDLNTCFS-------------LR 691
             PS+L    SLK LNL  C  LE + D     ++LE L+++ C +             LR
Sbjct: 781  LPSYLGHLVSLKSLNLDGCRRLENLPDTLQNLTSLETLEVSGCLNVNEFPRVSTSIEVLR 840

Query: 692  IIHESIG-------SLDKLITLQLDLCHNLEKLPSSL-KLKSLDSLSFTNCYKLEQLP-E 751
            I   SI        +L +L +L +     L  LP S+ +L+SL+ L  + C  LE  P E
Sbjct: 841  ISETSIEEIPARICNLSQLRSLDISENKRLASLPVSISELRSLEKLKLSGCSVLESFPLE 900

Query: 752  FDENMKSLRVMNLNGTAIRVLPSSIGYLIGLENLNLNDCANLTALPNEIHWLKSLEELHL 811
              + M  LR  +L+ T+I+ LP +IG L+ LE L  +    +   P  I  L  L+ L +
Sbjct: 901  ICQTMSCLRWFDLDRTSIKELPENIGNLVALEVLQASRTV-IRRAPWSIARLTRLQVLAI 960

Query: 812  RGCSKLDMFPPRSSLN--FSQESSYFKLTVLDLKNCNISNSDFLETLSNVCTSLEKLNLS 871
                    F P   L+      S +  L  L L N N+  ++   ++ N+   LE L+LS
Sbjct: 961  GN----SFFTPEGLLHSLCPPLSRFDDLRALSLSNMNM--TEIPNSIGNLWNLLE-LDLS 1020

Query: 872  GNKFSCLP-SLQNFKSLRFLELRNCKFLQNIIKLPHHLAR 880
            GN F  +P S++    L  L L NC+ LQ    LP  L R
Sbjct: 1021 GNNFEFIPASIKRLTRLNRLNLNNCQRLQ---ALPDELPR 1023

BLAST of Cucsa.275630 vs. TAIR10
Match: AT4G12010.1 (AT4G12010.1 Disease resistance protein (TIR-NBS-LRR class) family)

HSP 1 Score: 438.3 bits (1126), Expect = 1.2e-122
Identity = 313/925 (33.84%), Postives = 490/925 (52.97%), Query Frame = 1

Query: 1   QSSSSCSSNLKWSYDVFLSFRGEDTRNNFTSHLDRALREKGVNFFIDDKLERGGQISESL 60
           +SSS  S+     +DVFLSFRG DTRNNFT HL +ALR +G++ FIDD+L RG  ++ +L
Sbjct: 2   ESSSPSSAE----FDVFLSFRGFDTRNNFTGHLQKALRLRGIDSFIDDRLRRGDNLT-AL 61

Query: 61  LKSIDGSKISIIIFSKNYASSTWCLDELVKIVQCMKSMGHIVFPVFYKVDPSEVRKQTGG 120
              I+ SKI+II+FS NYA+S WCL ELVKI++C  S   +V P+FYKVD S+V KQ   
Sbjct: 62  FDRIEKSKIAIIVFSTNYANSAWCLRELVKILECRNSNQQLVVPIFYKVDKSDVEKQRNS 121

Query: 121 FGEALAKHEAN--ELMTNKVQPWKEALTTAASLSGW---DLATRKNEADLIHDLVKEVLS 180
           F       E     +   ++  WK AL +A+++ G+   +++T  +EA L+ ++  +   
Sbjct: 122 FAVPFKLPELTFPGVTPEEISSWKAALASASNILGYVVKEIST--SEAKLVDEIAVDTFK 181

Query: 181 ILNQTQLLHVAKHPVGIDSQLRAVEELASHDVPDGVNMVGIHGMGGIGKTTLAKALYNKI 240
            LN        +  VGI+S+L+ +E+L S +  D V+++GI GM GIGKTTLA  LY ++
Sbjct: 182 KLNDLAPSG-NEGLVGIESRLKNLEKLLSWEDLDTVHIIGIVGMVGIGKTTLADCLYGRM 241

Query: 241 AYQFEACCFLSNVRETLEQFKDLVQLQEKLLSEILKDNAWKVGNVHKGKNIIRDRLCSKK 300
             QF+  CFL+N+RE   +   L  L +KL S +L D   ++G           RL SK+
Sbjct: 242 RGQFDGSCFLTNIRENSGR-SGLESLLQKLFSTVLNDRDLEIGAPGNAHERFERRLKSKR 301

Query: 301 VLIILDDVDKDEQLDALVGERDWFGRGSKIIATTRDRHLLENHSFDIVYPIQLLDPKKSL 360
           +LI+LDDV+ ++Q+  L+G   W+  GS+II TTRD  L+E       Y +  L+ +++L
Sbjct: 302 LLIVLDDVNDEKQIRYLMGHCKWYQGGSRIIITTRDSKLIETIK-GRKYVLPKLNDREAL 361

Query: 361 ELFSLHAFKQNHPSSNYVDLSKFAVSYCKGLPLALVILGSLLHKRERKIWKSKLHELENS 420
           +LFSL+AF  + P   +  L+   + Y KG PLAL +LGS L +R+   W++KL  L++ 
Sbjct: 362 KLFSLNAFSNSFPLKEFEGLTNMVLDYAKGHPLALKVLGSDLCERDDLYWEAKLDRLKSR 421

Query: 421 LEPSVEAVFQIGFKELHERVKEIFLDISCFFVGEDINYSKDVLKACDLNPDYGIIILMDL 480
               +  V +  ++EL    K +FLDI+CFF  E+++Y   +L +  ++    +  L+D 
Sbjct: 422 SHGDIYEVLETSYEELTTEQKNVFLDIACFFRSENVDYVTSLLNSHGVDVSGVVKDLVDK 481

Query: 481 SLVTVEDGKIQMHDLIQQMGQTI--------------VRHESFEPAKRSRLWEAEGAIKI 540
            L+T+ D +I+MHD++Q M + I              +     +     RLW++E    +
Sbjct: 482 CLITLSDNRIEMHDMLQTMAKEISLKVETIGIRDCRWLSRHGNQCQWHIRLWDSEDICDL 541

Query: 541 LKEKSVSDFRQCFYL----LIAKDIYSEAFRNMKNLRLLIL----------QRVAYFPRN 600
           L E   +D  +  +L    L A  + ++AF+ M NL+ L +                 R 
Sbjct: 542 LTEGLGTDKIRGIFLDTSKLRAMRLSAKAFQGMYNLKYLKIYDSHCSRGCEAEFKLHLRR 601

Query: 601 IFEYLPNSLKWIEWSTFYVNQSSSISFSETPNFFATLNLEKLYLRGCTSLKGMRPSFRKF 660
              +LPN L ++ W   Y  QS  + F   P     L L    L               +
Sbjct: 602 GLSFLPNELTYLHWHG-YPLQSIPLDFD--PKNLVDLKLPHSQLE------------EIW 661

Query: 661 PSHLKFKSLKVLNLRDCLNLEEITDFSMASNLEILDLNTCFSLRIIHESIGSLDKLITLQ 720
                   LK ++L   +NL +    + A NLE L+L  C SL+ +  +I  L+KLI L 
Sbjct: 662 DDEKDVGMLKWVDLSHSINLRQCLGLANAHNLERLNLEGCTSLKKLPSTINCLEKLIYLN 721

Query: 721 LDLCHNLEKLPSSLKLKSLDSLSFTNCYKLEQLPEFDENMKSLRVMNLNGTAIRVLPSSI 780
           L  C +L  LP  +K +SL +L  + C  L++ P   EN++   V+ L+GT I+ LP SI
Sbjct: 722 LRDCTSLRSLPKGIKTQSLQTLILSGCSSLKKFPLISENVE---VLLLDGTVIKSLPESI 781

Query: 781 GYLIGLENLNLNDCANLTALPNEIHWLKSLEELHLRGCSKLDMFPPRSSLNFSQESSYFK 840
                L  LNL +C  L  L ++++ LK L+EL L GCS+L++FP    +    ES    
Sbjct: 782 QTFRRLALLNLKNCKKLKHLSSDLYKLKCLQELILSGCSQLEVFP---EIKEDMES---- 841

Query: 841 LTVLDLKNCNISNSDFLETLSNVCT-SL--EKLNLSGNKFSCLPSLQNFKSLRFLELRNC 890
           L +L + + +I+    +  LSN+ T SL     ++S + F   P+L     L  L L  C
Sbjct: 842 LEILLMDDTSITEMPKMMHLSNIKTFSLCGTSSHVSVSMFFMPPTL-GCSRLTDLYLSRC 886

BLAST of Cucsa.275630 vs. TAIR10
Match: AT4G11170.1 (AT4G11170.1 Disease resistance protein (TIR-NBS-LRR class) family)

HSP 1 Score: 432.6 bits (1111), Expect = 6.4e-121
Identity = 286/809 (35.35%), Postives = 438/809 (54.14%), Query Frame = 1

Query: 7   SSNLKWSYDVFLSFRGEDTRNNFTSHLDRALREKGVNFFIDDKLERGGQISESLLKSIDG 66
           SS+  W YDVF SFRGED RNNF SHL +    KG+  F DD ++R   I   L  +I  
Sbjct: 4   SSSNSWRYDVFPSFRGEDVRNNFLSHLLKEFESKGIVTFRDDHIKRSHTIGHELRAAIRE 63

Query: 67  SKISIIIFSKNYASSTWCLDELVKIVQCMKSMGHIVFPVFYKVDPSEVRKQTGGFGEALA 126
           SKIS+++FS+NYASS+WCLDEL++I++C +  G  V PVFYKVDPS++RKQTG FG +  
Sbjct: 64  SKISVVLFSENYASSSWCLDELIEIMKCKEEQGLKVMPVFYKVDPSDIRKQTGKFGMSFL 123

Query: 127 KHEANELMTNKVQPWKEALTTAASLSGWDLATRKNEADLIHDLVKEVLSILNQTQLLHVA 186
           +    +    +   W+ ALT AA++ G       NEA  I  + K+VL  LN T      
Sbjct: 124 ETCCGK-TEERQHNWRRALTDAANILGDHPQNWDNEAYKITTISKDVLEKLNATPSRDF- 183

Query: 187 KHPVGIDSQLRAVEELASHDVPDGVNMVGIHGMGGIGKTTLAKALYNKIAYQFEACCFLS 246
              VG+++ +  +E L   +   GV +VGI G  G+GKTT+A+ALYN+    F    F+ 
Sbjct: 184 NDLVGMEAHIAKMESLLCLE-SQGVRIVGIWGPAGVGKTTIARALYNQYHENFNLSIFME 243

Query: 247 NVRET-----LEQFKDLVQLQEKLLSEILKDNAWKVGNVHKGKNIIRDRLCSKKVLIILD 306
           NVRE+     L+ +   + LQ++ LS++L     +V ++      I +RL S+KVLIILD
Sbjct: 244 NVRESYGEAGLDDYGLKLHLQQRFLSKLLDQKDLRVRHL----GAIEERLKSQKVLIILD 303

Query: 307 DVDKDEQLDALVGERDWFGRGSKIIATTRDRHLLENHSFDIVYPIQLLDPKKSLELFSLH 366
           DVD  EQL AL  E  WFG  S+I+ TT+++ LL +H  + +Y +     +++L +F  H
Sbjct: 304 DVDNIEQLKALAKENQWFGNKSRIVVTTQNKQLLVSHDINHMYQVAYPSKQEALTIFCQH 363

Query: 367 AFKQNHPSSNYVDLSKFAVSYCKGLPLALVILGSLLHKRERKIWKSKLHELENSLEPSVE 426
           AFKQ+ PS +   L+    +    LPLAL +LGS +  + ++ W+  L  L++ L+  VE
Sbjct: 364 AFKQSSPSDDLKHLAIEFTTLAGHLPLALRVLGSFMRGKGKEEWEFSLPTLKSRLDGEVE 423

Query: 427 AVFQIGFKELHERVKEIFLDISCFFVGEDINYSKDVLKA-CDLNPDYGIIILMDLSLV-T 486
            V ++G+  LH+  K++FL I+C F G+  NY K ++ A  D    +G+ +L D SL+  
Sbjct: 424 KVLKVGYDGLHDHEKDLFLHIACIFSGQHENYLKQMIIANNDTYVSFGLQVLADKSLIQK 483

Query: 487 VEDGKIQMHDLIQQMGQTIVRHES-FEPAKRSRLWEAEGAIKILKEKSVSDFRQCFYL-- 546
            E+G+I+MH L++Q+G+ +VR +S +EP KR  L  A+    +L   + +       L  
Sbjct: 484 FENGRIEMHSLLRQLGKEVVRKQSIYEPGKRQFLMNAKETCGVLSNNTGTGTVLGISLDM 543

Query: 547 -LIAKDIY--SEAFRNMKNLRLL----------ILQRVAYFPRNIFEYLPNSLKWIEWST 606
             I +++Y   + F  M+NL  L           ++     P     YLP  L+ + W  
Sbjct: 544 CEIKEELYISEKTFEEMRNLVYLKFYMSSPIDDKMKVKLQLPEEGLSYLP-QLRLLHWDA 603

Query: 607 FYVNQSSSISFSETPNFFATLNLEKLYLRGCTSLKGMRPSFRKFPSHLKFKSLKVLNLRD 666
           + +    S   S  P     LN+    L+   S  G++P           ++L+ +NL  
Sbjct: 604 YPLEFFPS---SFRPECLVELNMSHSKLKKLWS--GVQP----------LRNLRTMNLNS 663

Query: 667 CLNLEEITDFSMASNLEILDLNTCFSLRIIHESIGSLDKLITLQLDLCHNLEKLPSSLKL 726
             NLE + +   A+ L  LDL  C SL  +  SI +L  LI L++  C  LE +P+++ L
Sbjct: 664 SRNLEILPNLMEATKLNRLDLGWCESLVELPSSIKNLQHLILLEMSCCKKLEIIPTNINL 723

Query: 727 KSLDSLSFTNCYKLEQLPEFDENMKSLRVMNLNGTAIRVLPSSIGYLIGLENLNLNDCAN 786
            SL+ L F  C +L+  PE   N   +R++NL GTAI  +P S+ Y   ++ +    C  
Sbjct: 724 PSLEVLHFRYCTRLQTFPEISTN---IRLLNLIGTAITEVPPSVKYWSKIDEI----CME 782

Query: 787 LTALPNEIHWLKSLEELHLRGCSKLDMFP 793
              +   +H    LE+L LR   +L+  P
Sbjct: 784 RAKVKRLVHVPYVLEKLCLRENKELETIP 782

BLAST of Cucsa.275630 vs. TAIR10
Match: AT5G18360.1 (AT5G18360.1 Disease resistance protein (TIR-NBS-LRR class) family)

HSP 1 Score: 431.8 bits (1109), Expect = 1.1e-120
Identity = 312/955 (32.67%), Postives = 490/955 (51.31%), Query Frame = 1

Query: 1   QSSSSCSSNLKWSYDVFLSFRGEDTRNNFTSHLDRALREKGVNFFIDDKLERGGQISESL 60
           +SSS    +  W + VF SF G+D R  F SHL +  R KG+  FID+ ++R   IS  L
Sbjct: 3   ESSSLSLQSCNWRHHVFPSFSGKDVRRTFLSHLLKEFRRKGIRTFIDNDIKRSQMISSEL 62

Query: 61  LKSIDGSKISIIIFSKNYASSTWCLDELVKIVQCMKSMGHIVFPVFYKVDPSEVRKQTGG 120
           +++I  S+I++++ S+ YASS+WCL+ELV+I    K +  ++ PVFY+VDPS+VRK+TG 
Sbjct: 63  VRAIRESRIAVVVLSRTYASSSWCLNELVEI----KKVSQMIMPVFYEVDPSDVRKRTGE 122

Query: 121 FGEALAKHEANELMTNKVQPWKEALTTAASLSGWDLATRKNEADLIHDLVKEVLSILNQT 180
           FG+A  +    +      Q W+EAL   A+++G       NEADLI  +   +   LN T
Sbjct: 123 FGKAFEEACERQPDEEVKQKWREALVYIANIAGESSQNWDNEADLIDKIAMSISYELNST 182

Query: 181 QLLHVAKHPVGIDSQLRAVEELASHDVPDGVNMVGIHGMGGIGKTTLAKALYNKIAYQFE 240
            L   + + VGID+ +R ++ L   +  + V MVGI G  GIGKTT+A+AL+N+++  F+
Sbjct: 183 -LSRDSYNLVGIDNHMRELDSLLCLESTE-VKMVGIWGPAGIGKTTIARALFNRLSENFQ 242

Query: 241 ACCFLSNVRET-----LEQFKDLVQLQEKLLSEILKDNAWKVGNVHKGKNIIRDRLCSKK 300
              F+ NV+ +     L+ +   ++LQE+ LSE++     K+ ++     ++++RL   K
Sbjct: 243 HTIFMENVKGSSRTSELDAYGFQLRLQEQFLSEVIDHKHMKIHDL----GLVKERLQDLK 302

Query: 301 VLIILDDVDKDEQLDALVGERDWFGRGSKIIATTRDRHLLENHSFDIVYPIQLLDPKKSL 360
           VL++LDDVDK EQLDALV +  WFG GS+II TT ++ LL  H    +Y +       SL
Sbjct: 303 VLVVLDDVDKLEQLDALVKQSQWFGSGSRIIVTTENKQLLRAHGITCIYELGFPSRSDSL 362

Query: 361 ELFSLHAFKQNHPSSNYVDLSKFAVSYCKGLPLALVILGSLLHKRERKIWKSKLHELENS 420
           ++F  +AF ++      ++L+         LPLAL +LGS L    +   KS L  L  S
Sbjct: 363 QIFCQYAFGESSAPDGCIELATEITKLAGYLPLALKVLGSSLRGMSKDEQKSALPRLRTS 422

Query: 421 LEPSVEAVFQIGFKELHERVKEIFLDISCFFVGEDINYSKDVLKACDLNPDYGIIILMDL 480
           L   +  V ++G+  +H++ K IFL I+C F GE+++Y K +L +  L+  +G+ +L   
Sbjct: 423 LNEDIRNVLRVGYDGIHDKDKVIFLHIACLFNGENVDYVKQILASSGLDVTFGLQVLTSR 482

Query: 481 SLVTVE--DGKIQMHDLIQQMGQTIVRHESF-EPAKRSRLWEAEGAIKILKEKSVSDFRQ 540
           SL+ +   +  I MH+L++Q+G+ IV  +S  EP KR  L +A     +L + + +    
Sbjct: 483 SLIHISRCNRTITMHNLLEQLGREIVCEQSIAEPGKRQFLMDASEIYDVLADNTGTGAVL 542

Query: 541 CFYLLIAK----DIYSEAFRNMKNLRLLIL--------QRVAYFPRNIFEYLPNSLKWIE 600
              L I+K     +   AF  M NL  L          Q   + PR + +YLP  L+ + 
Sbjct: 543 GISLDISKINELFLNERAFGGMHNLLFLRFYKSSSSKDQPELHLPRGL-DYLPRKLRLLH 602

Query: 601 WSTFYVNQSSSISFSETPNFFATLNLEKLYLRGCTSLKGMRPSFRKFPSHLKFKSLKVLN 660
           W  F     +S+  S  P F   +N+ +  L      +G +P           +SLK ++
Sbjct: 603 WDAF---PMTSMPLSFCPQFLVVINIRESQLE--KLWEGTQP----------LRSLKQMD 662

Query: 661 LRDCLNLEEITDFSMASNLEILDLNTCFSLRIIHESIGSLDKLITLQLDLCHNLEKLPSS 720
           L    NL+EI D S A N+E L L+ C SL ++  SI +L+KL+ L +  C  LE +P +
Sbjct: 663 LSKSENLKEIPDLSKAVNIEELCLSYCGSLVMLPSSIKNLNKLVVLDMKYCSKLEIIPCN 722

Query: 721 LKLKSLDSLSFTNCYKLEQLPEFDENMKSLRVMNLNGTAIRVLPSSIGYLIGLENLNLND 780
           + L+SL  L+   C +LE  PE       +  ++L+ TAI  +P+++     L  L+++ 
Sbjct: 723 MDLESLSILNLDGCSRLESFPEIS---SKIGFLSLSETAIEEIPTTVASWPCLAALDMSG 782

Query: 781 CANL---TALPNEIHWL----KSLEELHLRGCSKLDMFPPRSSLNFSQESSYFKLTVLDL 840
           C NL     LP  I WL      +EE+ L            S LN    +S  KL     
Sbjct: 783 CKNLKTFPCLPKTIEWLDLSRTEIEEVPL-------WIDKLSKLNKLLMNSCMKLR---- 842

Query: 841 KNCNISNSDFLETLSNVCTSLEKLNLSGNKFSCLPSLQNFKSLRF-----LELRNCKFLQ 900
                S S  + TL ++ T    L+  G K      ++ F+S RF     +E+RN   +Q
Sbjct: 843 -----SISSGISTLEHIKT----LDFLGCKNIVSFPVEIFESSRFCHNLVMEMRN---IQ 890

Query: 901 NIIKLPHHLARVNASGSELLAIRPDCIADMIYLHANDRHHIKVLFPNTTSKFVSK 924
           N       L R     +  +   PDCI            H K+ F N++    SK
Sbjct: 903 N-----PDLPRPFYFKNNYIDTIPDCIT----------RHCKLPFLNSSGSISSK 890

BLAST of Cucsa.275630 vs. NCBI nr
Match: gi|778708554|ref|XP_011656234.1| (PREDICTED: TMV resistance protein N-like [Cucumis sativus])

HSP 1 Score: 1257.3 bits (3252), Expect = 0.0e+00
Identity = 695/952 (73.00%), Postives = 752/952 (78.99%), Query Frame = 1

Query: 1   QSSSSCSSNLKWSYDVFLSFRGEDTRNNFTSHLDRALREKGVNFFIDDKLERGGQISESL 60
           QSSSSCSSNLKWSYDVFLSFRGEDTRNNFTSHLDRALREKGVNFFIDDKLERGGQISESL
Sbjct: 10  QSSSSCSSNLKWSYDVFLSFRGEDTRNNFTSHLDRALREKGVNFFIDDKLERGGQISESL 69

Query: 61  LKSIDGSKISIIIFSKNYASSTWCLDELVKIVQCMKSMGHIVFPVFYKVDPSEVRKQTGG 120
           LKSIDGSKISIIIFSKNYASSTWCLDELVKIVQCMKSMGHIVFPVFYKVDPSEVRKQTGG
Sbjct: 70  LKSIDGSKISIIIFSKNYASSTWCLDELVKIVQCMKSMGHIVFPVFYKVDPSEVRKQTGG 129

Query: 121 FGEALAKHEANELMTNKVQPWKEALTTAASLSGWDLATRKNEADLIHDLVKEVLSILNQT 180
           FGEALAKHEANELMTNKVQPWKEALTTAASLSGWDLATRKNEADLIHDLVKEVLSILNQT
Sbjct: 130 FGEALAKHEANELMTNKVQPWKEALTTAASLSGWDLATRKNEADLIHDLVKEVLSILNQT 189

Query: 181 QLLHVAKHPVGIDSQLRAVEELASHDVPDGVNMVGIHGMGGIGKTTLAKALYNKIAYQFE 240
           QLLHVAKHPVGIDSQLRAVEELASHDVPDGVNMVGIHGMGGIGKTTLAKALYNKIAYQFE
Sbjct: 190 QLLHVAKHPVGIDSQLRAVEELASHDVPDGVNMVGIHGMGGIGKTTLAKALYNKIAYQFE 249

Query: 241 ACCFLSNVRETLEQFKDLVQLQEKLLSEILKDNAWKVGNVHKGKNIIRDRLCSKKVLIIL 300
           ACCFLSNVRETLEQFKDLVQLQEKLLSEILKDNAWKVGNVHKGKNIIRDRLCSKKVLIIL
Sbjct: 250 ACCFLSNVRETLEQFKDLVQLQEKLLSEILKDNAWKVGNVHKGKNIIRDRLCSKKVLIIL 309

Query: 301 DDVDKDEQLDALVGERDWFGRGSKIIATTRDRHLLENHSFDIVYPIQLLDPKKSLELFSL 360
           DDVDKDEQLDALVGERDWFGRGSKIIATTRDRHLLENHSFDIVYPIQLLDPKKSLELFSL
Sbjct: 310 DDVDKDEQLDALVGERDWFGRGSKIIATTRDRHLLENHSFDIVYPIQLLDPKKSLELFSL 369

Query: 361 HAFKQNHPSSNYVDLSKFAVSYCKGLPLALVILGSLLHKRERKIWKSKLHELENSLEPSV 420
           HAFKQNHPSSNYVDLSKFAVSYCKGLPLALVILGSLLHKRERKIWKSKLHELENSLEPSV
Sbjct: 370 HAFKQNHPSSNYVDLSKFAVSYCKGLPLALVILGSLLHKRERKIWKSKLHELENSLEPSV 429

Query: 421 EAVFQIGFKELHERVKEIFLDISCFFVGEDINYSKDVLKACDLNPDYGIIILMDLSLVTV 480
           EAVFQIGFKELHERVKEIFLDISCFFVGEDINYSKDVLKACDLNPDYGIIILMDLSLVTV
Sbjct: 430 EAVFQIGFKELHERVKEIFLDISCFFVGEDINYSKDVLKACDLNPDYGIIILMDLSLVTV 489

Query: 481 EDGKIQMHDLIQQMGQTIVRHESFEPAKRSRLWEAEGAIKILKEKSVSDFRQCFYLLI-- 540
           EDGKIQMHDLIQQMGQTIVRHESFEPAKRSRLWEAEGAIKILKEKS +   +   L +  
Sbjct: 490 EDGKIQMHDLIQQMGQTIVRHESFEPAKRSRLWEAEGAIKILKEKSGTKAVKAIKLDLHY 549

Query: 541 ---AKDIYSEAFRNMKNLRLLILQRVAYFPRNIFEYLPNSLKWIEWSTFYVNQSSSISFS 600
               K + +EAFRNMKNLRLLILQRVAYFP+NIFEYLPNSLKWIEWSTFYVNQSSSISFS
Sbjct: 550 KPWLKIVEAEAFRNMKNLRLLILQRVAYFPKNIFEYLPNSLKWIEWSTFYVNQSSSISFS 609

Query: 601 ETPNFFATLNLEKLYLRGCTSLKGMRPSFRKFPSHLKFKSLKVLNLRDCLNLEEITDFSM 660
                     L  L ++G  + K  R +F         K++K ++L  C  L+E  +FS 
Sbjct: 610 ------VKGRLVGLVMKGVVN-KQPRIAFE------NCKTMKHVDLSYCGTLKETPNFSA 669

Query: 661 ASNLEILDLNTCFSLRIIHESIGSLDKLITLQLDLCHNLEKLPSS-LKLKSLDSLSFTNC 720
             NLE L L  C SL++IHES+ SL KL+TL L+ C NLEK PSS L LKSL+ L+ + C
Sbjct: 670 TLNLEKLYLRGCTSLKVIHESVASLSKLVTLDLEGCDNLEKFPSSYLMLKSLEVLNLSRC 729

Query: 721 YKLEQLPEF--DENMKSLRVMNLNGTAIRVLPSSIG-YLIGLENLNLNDCANLTALPNEI 780
            K+E++P+     N+K L +   +   +R++  SIG  L  L  L+L  C NL  LP   
Sbjct: 730 RKIEEIPDLSASSNLKELYLRECD--RLRIIHDSIGRSLDKLIILDLEGCKNLERLPIYT 789

Query: 781 HWLKSLEELHLRGCSKLDMFPPRSSLNFSQESSYFKLTVLDLKNC-------NISNSDFL 840
           + L+SLE L+L  C KL+ F   S   F     +  L VL+L++C       + S +  L
Sbjct: 790 NKLESLELLNLASCLKLETFFDSSFRKFPSHLKFKSLKVLNLRDCLNLEEITDFSMASNL 849

Query: 841 ETLS-NVCTSLE---------------KLNLSGNKFSCLPSLQNFKSLRFLELRNCKFLQ 900
           E L  N C SL                +L+L  N    LPS    KSL  L   NC  L+
Sbjct: 850 EILDLNTCFSLRIIHESIGSLDKLITLQLDLCHN-LEKLPSSLKLKSLDSLSFTNCYKLE 909

Query: 901 NIIKLPHHLARVNASGSELLAIR--PDCIADMI---YLHANDRHHIKVLFPN 916
            + +   ++  +        AIR  P  I  +I    L+ ND  ++  L PN
Sbjct: 910 QLPEFDENMKSLRVMNLNGTAIRVLPSSIGYLIGLENLNLNDCANLTAL-PN 944

BLAST of Cucsa.275630 vs. NCBI nr
Match: gi|778708554|ref|XP_011656234.1| (PREDICTED: TMV resistance protein N-like [Cucumis sativus])

HSP 1 Score: 379.4 bits (973), Expect = 1.8e-101
Identity = 194/212 (91.51%), Postives = 198/212 (93.40%), Query Frame = 1

Query: 605 NLEKLYLRGCTSLKGM-RPSFRKFPSHLKFKSLKVLNLRDCLNLEEITDFSMASNLEILD 664
           +LE L L  C  L+     SFRKFPSHLKFKSLKVLNLRDCLNLEEITDFSMASNLEILD
Sbjct: 779 SLELLNLASCLKLETFFDSSFRKFPSHLKFKSLKVLNLRDCLNLEEITDFSMASNLEILD 838

Query: 665 LNTCFSLRIIHESIGSLDKLITLQLDLCHNLEKLPSSLKLKSLDSLSFTNCYKLEQLPEF 724
           LNTCFSLRIIHESIGSLDKLITLQLDLCHNLEKLPSSLKLKSLDSLSFTNCYKLEQLPEF
Sbjct: 839 LNTCFSLRIIHESIGSLDKLITLQLDLCHNLEKLPSSLKLKSLDSLSFTNCYKLEQLPEF 898

Query: 725 DENMKSLRVMNLNGTAIRVLPSSIGYLIGLENLNLNDCANLTALPNEIHWLKSLEELHLR 784
           DENMKSLRVMNLNGTAIRVLPSSIGYLIGLENLNLNDCANLTALPNEIHWLKSLEELHLR
Sbjct: 899 DENMKSLRVMNLNGTAIRVLPSSIGYLIGLENLNLNDCANLTALPNEIHWLKSLEELHLR 958

Query: 785 GCSKLDMFPPRSSLNFSQESSYFKLTVLDLKN 816
           GCSKLDMFPPRSSLNFSQESSYFKLT L + +
Sbjct: 959 GCSKLDMFPPRSSLNFSQESSYFKLTELQVSS 990

HSP 2 Score: 1257.3 bits (3252), Expect = 0.0e+00
Identity = 695/952 (73.00%), Postives = 752/952 (78.99%), Query Frame = 1

Query: 1   QSSSSCSSNLKWSYDVFLSFRGEDTRNNFTSHLDRALREKGVNFFIDDKLERGGQISESL 60
           QSSSSCSSNLKWSYDVFLSFRGEDTRNNFTSHLDRALREKGVNFFIDDKLERGGQISESL
Sbjct: 10  QSSSSCSSNLKWSYDVFLSFRGEDTRNNFTSHLDRALREKGVNFFIDDKLERGGQISESL 69

Query: 61  LKSIDGSKISIIIFSKNYASSTWCLDELVKIVQCMKSMGHIVFPVFYKVDPSEVRKQTGG 120
           LKSIDGSKISIIIFSKNYASSTWCLDELVKIVQCMKSMGHIVFPVFYKVDPSEVRKQTGG
Sbjct: 70  LKSIDGSKISIIIFSKNYASSTWCLDELVKIVQCMKSMGHIVFPVFYKVDPSEVRKQTGG 129

Query: 121 FGEALAKHEANELMTNKVQPWKEALTTAASLSGWDLATRKNEADLIHDLVKEVLSILNQT 180
           FGEALAKHEANELMTNKVQPWKEALTTAASLSGWDLATRKNEADLIHDLVKEVLSILNQT
Sbjct: 130 FGEALAKHEANELMTNKVQPWKEALTTAASLSGWDLATRKNEADLIHDLVKEVLSILNQT 189

Query: 181 QLLHVAKHPVGIDSQLRAVEELASHDVPDGVNMVGIHGMGGIGKTTLAKALYNKIAYQFE 240
           QLLHVAKHPVGIDSQLRAVEELASHDVPDGVNMVGIHGMGGIGKTTLAKALYNKIAYQFE
Sbjct: 190 QLLHVAKHPVGIDSQLRAVEELASHDVPDGVNMVGIHGMGGIGKTTLAKALYNKIAYQFE 249

Query: 241 ACCFLSNVRETLEQFKDLVQLQEKLLSEILKDNAWKVGNVHKGKNIIRDRLCSKKVLIIL 300
           ACCFLSNVRETLEQFKDLVQLQEKLLSEILKDNAWKVGNVHKGKNIIRDRLCSKKVLIIL
Sbjct: 250 ACCFLSNVRETLEQFKDLVQLQEKLLSEILKDNAWKVGNVHKGKNIIRDRLCSKKVLIIL 309

Query: 301 DDVDKDEQLDALVGERDWFGRGSKIIATTRDRHLLENHSFDIVYPIQLLDPKKSLELFSL 360
           DDVDKDEQLDALVGERDWFGRGSKIIATTRDRHLLENHSFDIVYPIQLLDPKKSLELFSL
Sbjct: 310 DDVDKDEQLDALVGERDWFGRGSKIIATTRDRHLLENHSFDIVYPIQLLDPKKSLELFSL 369

Query: 361 HAFKQNHPSSNYVDLSKFAVSYCKGLPLALVILGSLLHKRERKIWKSKLHELENSLEPSV 420
           HAFKQNHPSSNYVDLSKFAVSYCKGLPLALVILGSLLHKRERKIWKSKLHELENSLEPSV
Sbjct: 370 HAFKQNHPSSNYVDLSKFAVSYCKGLPLALVILGSLLHKRERKIWKSKLHELENSLEPSV 429

Query: 421 EAVFQIGFKELHERVKEIFLDISCFFVGEDINYSKDVLKACDLNPDYGIIILMDLSLVTV 480
           EAVFQIGFKELHERVKEIFLDISCFFVGEDINYSKDVLKACDLNPDYGIIILMDLSLVTV
Sbjct: 430 EAVFQIGFKELHERVKEIFLDISCFFVGEDINYSKDVLKACDLNPDYGIIILMDLSLVTV 489

Query: 481 EDGKIQMHDLIQQMGQTIVRHESFEPAKRSRLWEAEGAIKILKEKSVSDFRQCFYLLI-- 540
           EDGKIQMHDLIQQMGQTIVRHESFEPAKRSRLWEAEGAIKILKEKS +   +   L +  
Sbjct: 490 EDGKIQMHDLIQQMGQTIVRHESFEPAKRSRLWEAEGAIKILKEKSGTKAVKAIKLDLHY 549

Query: 541 ---AKDIYSEAFRNMKNLRLLILQRVAYFPRNIFEYLPNSLKWIEWSTFYVNQSSSISFS 600
               K + +EAFRNMKNLRLLILQRVAYFP+NIFEYLPNSLKWIEWSTFYVNQSSSISFS
Sbjct: 550 KPWLKIVEAEAFRNMKNLRLLILQRVAYFPKNIFEYLPNSLKWIEWSTFYVNQSSSISFS 609

Query: 601 ETPNFFATLNLEKLYLRGCTSLKGMRPSFRKFPSHLKFKSLKVLNLRDCLNLEEITDFSM 660
                     L  L ++G  + K  R +F         K++K ++L  C  L+E  +FS 
Sbjct: 610 ------VKGRLVGLVMKGVVN-KQPRIAFE------NCKTMKHVDLSYCGTLKETPNFSA 669

Query: 661 ASNLEILDLNTCFSLRIIHESIGSLDKLITLQLDLCHNLEKLPSS-LKLKSLDSLSFTNC 720
             NLE L L  C SL++IHES+ SL KL+TL L+ C NLEK PSS L LKSL+ L+ + C
Sbjct: 670 TLNLEKLYLRGCTSLKVIHESVASLSKLVTLDLEGCDNLEKFPSSYLMLKSLEVLNLSRC 729

Query: 721 YKLEQLPEF--DENMKSLRVMNLNGTAIRVLPSSIG-YLIGLENLNLNDCANLTALPNEI 780
            K+E++P+     N+K L +   +   +R++  SIG  L  L  L+L  C NL  LP   
Sbjct: 730 RKIEEIPDLSASSNLKELYLRECD--RLRIIHDSIGRSLDKLIILDLEGCKNLERLPIYT 789

Query: 781 HWLKSLEELHLRGCSKLDMFPPRSSLNFSQESSYFKLTVLDLKNC-------NISNSDFL 840
           + L+SLE L+L  C KL+ F   S   F     +  L VL+L++C       + S +  L
Sbjct: 790 NKLESLELLNLASCLKLETFFDSSFRKFPSHLKFKSLKVLNLRDCLNLEEITDFSMASNL 849

Query: 841 ETLS-NVCTSLE---------------KLNLSGNKFSCLPSLQNFKSLRFLELRNCKFLQ 900
           E L  N C SL                +L+L  N    LPS    KSL  L   NC  L+
Sbjct: 850 EILDLNTCFSLRIIHESIGSLDKLITLQLDLCHN-LEKLPSSLKLKSLDSLSFTNCYKLE 909

Query: 901 NIIKLPHHLARVNASGSELLAIR--PDCIADMI---YLHANDRHHIKVLFPN 916
            + +   ++  +        AIR  P  I  +I    L+ ND  ++  L PN
Sbjct: 910 QLPEFDENMKSLRVMNLNGTAIRVLPSSIGYLIGLENLNLNDCANLTAL-PN 944

BLAST of Cucsa.275630 vs. NCBI nr
Match: gi|700196043|gb|KGN51220.1| (hypothetical protein Csa_5G494390 [Cucumis sativus])

HSP 1 Score: 555.8 bits (1431), Expect = 1.4e-154
Identity = 290/334 (86.83%), Postives = 301/334 (90.12%), Query Frame = 1

Query: 605  NLEKLYLRGCTSLKGM-RPSFRKFPSHLKFKSLKVLNLRDCLNLEEITDFSMASNLEILD 664
            +LE L L  C  L+     SFRKFPSHLKFKSLKVLNLRDCLNLEEITDFSMASNLEILD
Sbjct: 779  SLELLNLASCLKLETFFDSSFRKFPSHLKFKSLKVLNLRDCLNLEEITDFSMASNLEILD 838

Query: 665  LNTCFSLRIIHESIGSLDKLITLQLDLCHNLEKLPSSLKLKSLDSLSFTNCYKLEQLPEF 724
            LNTCFSLRIIHESIGSLDKLITLQLDLCHNLEKLPSSLKLKSLDSLSFTNCYKLEQLPEF
Sbjct: 839  LNTCFSLRIIHESIGSLDKLITLQLDLCHNLEKLPSSLKLKSLDSLSFTNCYKLEQLPEF 898

Query: 725  DENMKSLRVMNLNGTAIRVLPSSIGYLIGLENLNLNDCANLTALPNEIHWLKSLEELHLR 784
            DENMKSLRVMNLNGTAIRVLPSSIGYLIGLENLNLNDCANLTALPNEIHWLKSLEELHLR
Sbjct: 899  DENMKSLRVMNLNGTAIRVLPSSIGYLIGLENLNLNDCANLTALPNEIHWLKSLEELHLR 958

Query: 785  GCSKLDMFPPRSSLNFSQESSYFKLTVLDLKNCNISNSDFLETLSNVCTSLEKLNLSGNK 844
            GCSKLDMFPPRSSLNFSQESSYFKLTVLDLKNCNISNSDFLETLSNVCTSLEKLNLSGN 
Sbjct: 959  GCSKLDMFPPRSSLNFSQESSYFKLTVLDLKNCNISNSDFLETLSNVCTSLEKLNLSGNT 1018

Query: 845  FSCLPSLQNFKSLRFLELRNCKFLQNIIKLPHHLARVNASGSELLAIRPDCIADMIY--L 904
            FSCLPSLQNFKSLRFLELRNCKFLQNIIKLPHHLARVNASGSELLAIRPDCIADM++   
Sbjct: 1019 FSCLPSLQNFKSLRFLELRNCKFLQNIIKLPHHLARVNASGSELLAIRPDCIADMMFGKQ 1078

Query: 905  HANDRHHIKVLF--PNTTSKFVSKRFKSANVIMR 934
             A      KVLF   N   K+ +K+   +++ +R
Sbjct: 1079 DAEFSDSTKVLFITNNEIPKYCNKQTTRSSMSVR 1112

HSP 2 Score: 935.3 bits (2416), Expect = 8.6e-269
Identity = 536/887 (60.43%), Postives = 644/887 (72.60%), Query Frame = 1

Query: 1   QSSSSCSSNLKWSYDVFLSFRGEDTRNNFTSHLDRALREKGVNFFIDDKLERGGQISESL 60
           +SSSSCSSNL+WSYDVFLSFRGEDTR+ F SHL  ALR+KGVNFFIDDKL+RG QIS+SL
Sbjct: 10  ESSSSCSSNLRWSYDVFLSFRGEDTRDKFISHLVVALRQKGVNFFIDDKLDRGHQISKSL 69

Query: 61  LKSIDGSKISIIIFSKNYASSTWCLDELVKIVQCMKSMGHIVFPVFYKVDPSEVRKQTGG 120
           LKSI+ S+ISIIIFS+NYASSTWCLDELVKI++CM+S    V PVFY V P+EV KQTG 
Sbjct: 70  LKSIEESRISIIIFSQNYASSTWCLDELVKIIECMRSKKQRVLPVFYNVCPTEVVKQTGS 129

Query: 121 FGEALAKHEANELMTNKVQPWKEALTTAASLSGWDLATR-KNEADLIHDLVKEVLSILNQ 180
           FGEA+AK+E N LM NK+QPWKEALTTAA+LSGWDL    KNEA LIHDLV++V SIL Q
Sbjct: 130 FGEAMAKYETNRLMINKIQPWKEALTTAATLSGWDLPNYWKNEAHLIHDLVEKV-SILKQ 189

Query: 181 TQLLHVAKHPVGIDSQLRAVEELASHDVPD-GVNMVGIHGMGGIGKTTLAKALYNKIAYQ 240
           TQLL+VAKHPVGIDSQL+AVEE ASH V D GVNMVGIHGMGGIGKTTLAKALYNKI Y+
Sbjct: 190 TQLLNVAKHPVGIDSQLKAVEEFASHGVLDNGVNMVGIHGMGGIGKTTLAKALYNKITYE 249

Query: 241 FEACCFLSNVRETLEQFKDLVQLQEKLLSEILKDNAWKVGNVHKGKNIIRDRLCSKKVLI 300
           FEACCFLSNVRE  EQF  LVQLQEKLLSEI KDN  KV NVHKG NI++DRLCS+KVLI
Sbjct: 250 FEACCFLSNVREASEQFNGLVQLQEKLLSEIFKDNNLKVDNVHKGMNIMKDRLCSRKVLI 309

Query: 301 ILDDVDKDEQLDALVGERDWFGRGSKIIATTRDRHLLENHSFDIVYPIQLLDPKKSLELF 360
           +LDDVDKD+QLDALVG RDWFGRGSKII TTRDRHLLE +SFD ++PIQLLD  KSLELF
Sbjct: 310 VLDDVDKDDQLDALVGGRDWFGRGSKIIVTTRDRHLLERYSFDKIHPIQLLDYVKSLELF 369

Query: 361 SLHAFKQNHPSSNYVDLSKFAVSYCKGLPLALVILGSLLHKRERKIWKSKLHELENSLEP 420
             HAFKQNHPS +Y+DLS+  V YC GLPLALVILGSLL KR++KIWKSKL EL+N  EP
Sbjct: 370 CWHAFKQNHPSRDYLDLSELVVRYCNGLPLALVILGSLLCKRDQKIWKSKLDELKNFPEP 429

Query: 421 SVEAVFQIGFKELHER--VKEIFLDISCFFVGEDINYSKDVLKACDLNPDYGIIILMDLS 480
            +EAVFQI FK L E   VKEIFLDI CFFVGED++YSK+VLKACD   +  IIILMDLS
Sbjct: 430 GIEAVFQISFKRLEENPPVKEIFLDICCFFVGEDVSYSKNVLKACDPYLESRIIILMDLS 489

Query: 481 LVTVEDGKIQMHDLIQQMGQTIVRHESFEPAKRSRLWEAEGAIKILKEKSVSDFRQCFYL 540
           LVTVEDGKIQMHDLI+QMGQTIVR +S +P KRSRLW A+ A+K+L EKS +   +   L
Sbjct: 490 LVTVEDGKIQMHDLIRQMGQTIVRCKSSKPEKRSRLWVAKEAVKMLIEKSGTHKVKAIKL 549

Query: 541 LIAKD----IYSEAFRNMKNLRLLILQRVAYFPRNIFEYLPNSLKWIEWSTFYVNQSSSI 600
            +  +    + +EAFRNM+NLRLLILQ  A FP NIF+YLPN +KWIE+S      SS++
Sbjct: 550 DLRSNRPLIVEAEAFRNMENLRLLILQNAAKFPTNIFKYLPN-IKWIEYS------SSNV 609

Query: 601 SFSETPNFFATLNLEKLYLRGCTSLKGMRPSFRKFPSHL--KFKSLKVLNLRDCLNLEEI 660
            +    +F     L  L + G ++         K P  +    K LK ++L     LEE 
Sbjct: 610 QWYFPISFVVNGGLVGLVINGVSN---------KHPGIIFEDCKMLKHVDLSYWRLLEET 669

Query: 661 TDFSMASNLEILDLNTCFSLRIIHESIGSLDKLITLQLDLCHNLEKLPSS-LKLKSLDSL 720
            DFS+A NLE L L +C  L +IH SI SL KL+TL L+ C NLEKLPSS L LKSL+ L
Sbjct: 670 PDFSVALNLEKLYLRSCKRLEMIHGSIASLSKLVTLDLEGCENLEKLPSSFLMLKSLEVL 729

Query: 721 SFTNCYKLEQLPEFDENMKSLRVMNLNGT-AIRVLPSSIG-YLIGLENLNLNDCANLTAL 780
           + + C KL+++P+   +  SL+ ++L     +R++  S+G +L  L  L+   C NL  L
Sbjct: 730 NLSGCIKLKEIPDLSAS-SSLKELHLRECYNLRIIHDSVGRFLDKLVILDFEGCRNLERL 789

Query: 781 PNEIHWLKSLEELHLRGCSKLDMFPPRSSLNFSQESSYFKLTVLDLKNC-NISN-SDFLE 840
           P  I    S+E L+L  C K++         F     Y  L VL+L  C N+   +DF  
Sbjct: 790 PRYISKSGSIEVLNLDSCRKIEQIFDNYFEKFPSHLKYESLKVLNLSYCQNLKGITDF-- 849

Query: 841 TLSNVCTSLEKLNLSG--NKFSCLPSLQNFKSLRFLELRNCKFLQNI 871
              +  ++LE L+L G  +  +   S+ +   L  L+L +C  L+ +
Sbjct: 850 ---SFASNLEILDLRGCFSLRTIHESVGSLDKLIALKLDSCHLLEEL 873

BLAST of Cucsa.275630 vs. NCBI nr
Match: gi|659086568|ref|XP_008444002.1| (PREDICTED: TMV resistance protein N-like [Cucumis melo])

HSP 1 Score: 409.5 bits (1051), Expect = 1.7e-110
Identity = 210/297 (70.71%), Postives = 243/297 (81.82%), Query Frame = 1

Query: 605  NLEKLYLRGCTSLKGMRPS-FRKFPSHLKFKSLKVLNLRDCLNLEEITDFSMASNLEILD 664
            ++E L L  C  ++ +  + F KFPSHLK++SLKVLNL  C NL+ ITDFS ASNLEILD
Sbjct: 780  SIEVLNLDSCRKIEQIFDNYFEKFPSHLKYESLKVLNLSYCQNLKGITDFSFASNLEILD 839

Query: 665  LNTCFSLRIIHESIGSLDKLITLQLDLCHNLEKLPSSLKLKSLDSLSFTNCYKLEQLPEF 724
            L  CFSLR IHES+GSLDKLI L+LD CH LE+LPS L+LKSLDSLS TNCYKLEQLPEF
Sbjct: 840  LRGCFSLRTIHESVGSLDKLIALKLDSCHLLEELPSCLRLKSLDSLSLTNCYKLEQLPEF 899

Query: 725  DENMKSLRVMNLNGTAIRVLPSSIGYLIGLENLNLNDCANLTALPNEIHWLKSLEELHLR 784
            DENMKSLR MNL GTAIR LPSSI YLIGLENLNL+ C NL +LP+EIH LKSL+EL L 
Sbjct: 900  DENMKSLREMNLKGTAIRKLPSSIRYLIGLENLNLSYCTNLISLPSEIHLLKSLKELDLH 959

Query: 785  GCSKLDMFPPRSSLNFSQESSYFKLTVLDLKNCNISNSDFLETLSNVCTSLEKLNLSGNK 844
             CS+LDMFP  SSLNF Q+S +  LT+LDL+NCNISN+DFLE LSN CT+L++LNLSGNK
Sbjct: 960  ECSRLDMFPSGSSLNFPQQSLFSNLTILDLQNCNISNTDFLENLSNFCTTLKELNLSGNK 1019

Query: 845  FSCLPSLQNFKSLRFLELRNCKFLQNIIKLPHHLARVNASGSELLAIRPDCIADMIY 901
            F  LPSLQNF SLR LELRNCKFL+NI+K+PH L RV+ASG EL  I PD IAD+++
Sbjct: 1020 FCSLPSLQNFSSLRHLELRNCKFLRNIVKIPHCLTRVDASGCELFVISPDYIADIMF 1076

HSP 2 Score: 65.9 bits (159), Expect = 4.5e-07
Identity = 62/208 (29.81%), Postives = 96/208 (46.15%), Query Frame = 1

Query: 694 LEKLPSSLKLKSLDSLSFTNCYKLEQLPEFDENMKSLRVMNLNGTA-IRVLPSSIGYLIG 753
           LE+ P      +L+ L   +C +LE +     ++  L  ++L G   +  LPSS   L  
Sbjct: 649 LEETPDFSVALNLEKLYLRSCKRLEMIHGSIASLSKLVTLDLEGCENLEKLPSSFLMLKS 708

Query: 754 LENLNLNDCANLTALPNEIHWLKSLEELHLRGCSKLDMFPPRSSLNFSQESSYFKLTVLD 813
           LE LNL+ C  L  +P ++    SL+ELHLR C  L +      ++ S      KL +LD
Sbjct: 709 LEVLNLSGCIKLKEIP-DLSASSSLKELHLRECYNLRI------IHDSVGRFLDKLVILD 768

Query: 814 LKNCN--------ISNSDFLETLS-NVCTSLEKLNLSGNKFSCLPSLQNFKSLRFLELRN 873
            + C         IS S  +E L+ + C  +E+  +  N F   PS   ++SL+ L L  
Sbjct: 769 FEGCRNLERLPRYISKSGSIEVLNLDSCRKIEQ--IFDNYFEKFPSHLKYESLKVLNLSY 828

Query: 874 CKFLQNIIKLPHHLARVNASGSELLAIR 892
           C+ L+ I           AS  E+L +R
Sbjct: 829 CQNLKGITDFSF------ASNLEILDLR 841

HSP 3 Score: 921.8 bits (2381), Expect = 9.9e-265
Identity = 537/928 (57.87%), Postives = 643/928 (69.29%), Query Frame = 1

Query: 1   QSSSSCSSNLKWSYDVFLSFRGEDTRNNFTSHLDRALREKGVNFFIDDKLERGGQISESL 60
           +SSSSCSSN KWSYDVFLSFRGEDTR+ F SHLD ALR +GVNFFIDDKL+RG QIS+SL
Sbjct: 10  ESSSSCSSNSKWSYDVFLSFRGEDTRDKFISHLDLALRREGVNFFIDDKLDRGKQISKSL 69

Query: 61  LKSIDGSKISIIIFSKNYASSTWCLDELVKIVQCMKSMGHIVFPVFYKVDPSEVRKQTGG 120
           LKSI+GS+ISIIIFS+NYASSTWCLDE+VKI++CM+S    V PVFY V PSEV KQTG 
Sbjct: 70  LKSIEGSRISIIIFSQNYASSTWCLDEVVKIIECMRSKKQTVLPVFYNVSPSEVVKQTGI 129

Query: 121 FGEALAKHEANELMTNKVQPWKEALTTAASLSGWDLAT--RKNEADLIHDLVKEVLSILN 180
           FGEA AK+E N LMTNK+QPWKEALTTAA+LSGWDL    + NEA LI DLVK+V SIL 
Sbjct: 130 FGEAFAKYETNPLMTNKIQPWKEALTTAATLSGWDLGNYWKNNEAHLIQDLVKKV-SILK 189

Query: 181 QTQLLHVAKHPVGIDSQLRAVEELASHDVPD-GVNMVGIHGMGGIGKTTLAKALYNKIAY 240
           QTQLL+VAKHPV IDSQL+A+EELASH V D GVNMVGIHGMGGIGKTTLAKALYNKI Y
Sbjct: 190 QTQLLNVAKHPVAIDSQLKAIEELASHGVSDNGVNMVGIHGMGGIGKTTLAKALYNKITY 249

Query: 241 QFEACCFLSNVRETLEQFKDLVQLQEKLLSEILKDNAWKVGNVHKGKNIIRDRLCSKKVL 300
           QFEACCFLSNVRET EQF  LVQLQEKLL+EI KDN  KV NV KG NII+DRLCS+KVL
Sbjct: 250 QFEACCFLSNVRETSEQFNGLVQLQEKLLNEIFKDNNLKVDNVDKGMNIIKDRLCSRKVL 309

Query: 301 IILDDVDKDEQLDALVGERDWFGRGSKIIATTRDRHLLENHSFDIVYPIQLLDPKKSLEL 360
           ++LDDVDKD+QLDALVG RDWFGRGSKII TTRDRHLLE +SFD ++PIQLLD  KSLEL
Sbjct: 310 MVLDDVDKDDQLDALVGGRDWFGRGSKIIVTTRDRHLLETYSFDKIHPIQLLDCDKSLEL 369

Query: 361 FSLHAFKQNHPSSNYVDLSKFAVSYCKGLPLALVILGSLLHKRERKIWKSKLHELENSLE 420
           F  HAFKQ+HPS NY +L +  V YC GLPLALVILGSLL KR++ IWKSKL EL+N  E
Sbjct: 370 FCWHAFKQSHPSRNYSELPEL-VRYCNGLPLALVILGSLLCKRDQIIWKSKLDELKNFPE 429

Query: 421 PSVEAVFQIGFKELHER--VKEIFLDISCFFVGEDINYSKDVLKACDLNPDYGIIILMDL 480
           P +EAVFQI FK L E   VKEIFLDI CFFVGED++YSK+VLKACD   +  IIILMDL
Sbjct: 430 PGIEAVFQISFKRLPENPPVKEIFLDICCFFVGEDVSYSKNVLKACDPYLESRIIILMDL 489

Query: 481 SLVTVEDGKIQMHDLIQQMGQTIVRHESFEPAKRSRLWEAEGAIKILKEKSVSDFRQCFY 540
           SLVTVEDGKIQMHDLI+QMGQ IVR +SF+P KRSRLW A+ A+K+L EKS +   +   
Sbjct: 490 SLVTVEDGKIQMHDLIRQMGQMIVRRKSFKPEKRSRLWVAKEAVKMLIEKSGTHKVKAIK 549

Query: 541 LLIAKD----IYSEAFRNMKNLRLLILQRVAYFPRNIFEYLPNSLKWIEWSTFYVNQSSS 600
           L +  +    + +EAFRNM+NLRLLILQ  A  P NIF+YLPN +KWIE+S      SSS
Sbjct: 550 LDLRNNGSLIVEAEAFRNMENLRLLILQNAAKLPTNIFKYLPN-IKWIEYS------SSS 609

Query: 601 ISFSETPNFFATLNLEKLYLRGCTSLKGMRPSFRKFPSHL--KFKSLKVLNLRDCLNLEE 660
           + +    +F     L  L + G ++         K P  +    K LK ++L     LEE
Sbjct: 610 VRWYFPISFVVNGGLVGLVINGVSN---------KHPGIIFEDCKMLKHVDLSYWRLLEE 669

Query: 661 ITDFSMASNLEILDLNTCFSLRIIHESIGSLDKLITLQLDLCHNLEKLPSS-LKLKSLDS 720
             DFS A NLE L L +C  L++IH S+ SL KL+TL L+ C NLEKLPSS L LKSL+ 
Sbjct: 670 TPDFSAALNLEKLYLLSCKRLKMIHGSVASLSKLVTLDLEGCENLEKLPSSFLMLKSLEV 729

Query: 721 LSFTNCYKLEQLPEF--DENMKSLRVMNLNGTAIRVLPSSIG-YLIGLENLNLNDCANLT 780
           L+ + C KL+++P+     N+K L +       I +  S++G +L  L  L+L  C  L 
Sbjct: 730 LNLSGCIKLKEIPDLSASSNLKELHLRECYHLRI-IHDSAVGRFLDKLVILDLEGCKILE 789

Query: 781 ALPNEIHWLKSLEELHLRGCSKLDMFPPRSSLNFSQESSYFKLTVLDLKNC-NISNSDFL 840
            LP  I   KS+E ++L  C K++         F     +  L VL+L  C N+      
Sbjct: 790 RLPRYISNSKSIEVMNLDSCRKIEQLFDNYFEKFPSHLKFESLKVLNLSYCQNLKEITDF 849

Query: 841 ETLSNV-------CTSLEKLNLS--------------GNKFSCLPSLQNFKSLRFLELRN 892
              SN+       C SL  ++ S               ++   LPS    KSL  L L N
Sbjct: 850 SIASNLEIFDLRGCFSLRTIHKSVGSLDQLIALKLDFCHQLEELPSCLRLKSLDSLSLTN 909

BLAST of Cucsa.275630 vs. NCBI nr
Match: gi|700210236|gb|KGN65332.1| (hypothetical protein Csa_1G334920 [Cucumis sativus])

HSP 1 Score: 409.8 bits (1052), Expect = 1.3e-110
Identity = 233/406 (57.39%), Postives = 278/406 (68.47%), Query Frame = 1

Query: 503  SFEPAKRSRLWEAEGAIKILKEKSVS--------DFRQCFYLLIAKDIYSEAFRNMKNLR 562
            SF   K   +    G IK+ +   +S          R+C++L I  D  S   R +  L 
Sbjct: 702  SFLMLKSLEVLNLSGCIKLKEIPDLSASSNLKELHLRECYHLRIIHD--SAVGRFLDKLV 761

Query: 563  LLILQRVAYFPRNIFEYLPNSLKWIEWSTFYVNQSSSISFSETPNFFATLNLEKLYLRGC 622
            +L L+        I E LP           Y++ S SI   E  N  +   +E+L+    
Sbjct: 762  ILDLEGC-----KILERLPR----------YISNSKSI---EVMNLDSCRKIEQLFDN-- 821

Query: 623  TSLKGMRPSFRKFPSHLKFKSLKVLNLRDCLNLEEITDFSMASNLEILDLNTCFSLRIIH 682
                     F KFPSHLKF+SLKVLNL  C NL+EITDFS+ASNLEI DL  CFSLR IH
Sbjct: 822  --------YFEKFPSHLKFESLKVLNLSYCQNLKEITDFSIASNLEIFDLRGCFSLRTIH 881

Query: 683  ESIGSLDKLITLQLDLCHNLEKLPSSLKLKSLDSLSFTNCYKLEQLPEFDENMKSLRVMN 742
            +S+GSLD+LI L+LD CH LE+LPS L+LKSLDSLS TNCYK+EQLPEFDENMKSLR MN
Sbjct: 882  KSVGSLDQLIALKLDFCHQLEELPSCLRLKSLDSLSLTNCYKIEQLPEFDENMKSLREMN 941

Query: 743  LNGTAIRVLPSSIGYLIGLENLNLNDCANLTALPNEIHWLKSLEELHLRGCSKLDMFPPR 802
            L GTAIR LP+SI YLIGLENL L+ C NL +LP+EIH LKSL+EL LR CS+LDM P  
Sbjct: 942  LKGTAIRKLPTSIRYLIGLENLILSYCTNLISLPSEIHLLKSLKELDLRECSRLDMLPSG 1001

Query: 803  SSLNFSQESSYFKLTVLDLKNCNISNSDFLETLSNVCTSLEKLNLSGNKFSCLPSLQNFK 862
            SSLNF Q S    LT+LDL+NCNISNSDFLE LSN CT+L++LNLSGNKF CLPSL+NF 
Sbjct: 1002 SSLNFPQRSLCSNLTILDLQNCNISNSDFLENLSNFCTTLKELNLSGNKFCCLPSLKNFT 1061

Query: 863  SLRFLELRNCKFLQNIIKLPHHLARVNASGSELLAIRPDCIADMIY 901
            SLR LELRNCKFL+NI+K+PH L R++ASG ELL I PD IADM++
Sbjct: 1062 SLRLLELRNCKFLRNIVKIPHCLKRMDASGCELLVISPDYIADMMF 1077

HSP 2 Score: 122.1 bits (305), Expect = 5.3e-24
Identity = 105/310 (33.87%), Postives = 147/310 (47.42%), Query Frame = 1

Query: 596 ETPNFFATLNLEKLYLRGCTSLKGMRPS-----------------FRKFP-SHLKFKSLK 655
           ETP+F A LNLEKLYL  C  LK +  S                   K P S L  KSL+
Sbjct: 651 ETPDFSAALNLEKLYLLSCKRLKMIHGSVASLSKLVTLDLEGCENLEKLPSSFLMLKSLE 710

Query: 656 VLNLRDCLNLEEITDFSMASNLEILDLNTCFSLRIIHES-IGS-LDKLITLQLDLCHNLE 715
           VLNL  C+ L+EI D S +SNL+ L L  C+ LRIIH+S +G  LDKL+ L L+ C  LE
Sbjct: 711 VLNLSGCIKLKEIPDLSASSNLKELHLRECYHLRIIHDSAVGRFLDKLVILDLEGCKILE 770

Query: 716 KLPSSL-KLKSLDSLSFTNCYKLEQL-----PEFDENMK--SLRVMNLNGTAIRVLPSSI 775
           +LP  +   KS++ ++  +C K+EQL      +F  ++K  SL+V+NL+        +  
Sbjct: 771 RLPRYISNSKSIEVMNLDSCRKIEQLFDNYFEKFPSHLKFESLKVLNLSYCQNLKEITDF 830

Query: 776 GYLIGLENLNLNDCANLTALPNEIHWLKSLEELHLRGCSKLDMFPPRSSLNFSQESSYFK 835
                LE  +L  C +L  +   +  L  L  L L  C +L+  P    L          
Sbjct: 831 SIASNLEIFDLRGCFSLRTIHKSVGSLDQLIALKLDFCHQLEELPSCLRLK--------S 890

Query: 836 LTVLDLKNCNISNSDFLETLSNVCTSLEKLNLSGNKFSCLPSLQNFKSLRFLELRNCKFL 878
           L  L L NC     + L        SL ++NL G     LP+  + + L  LE     + 
Sbjct: 891 LDSLSLTNC--YKIEQLPEFDENMKSLREMNLKGTAIRKLPT--SIRYLIGLENLILSYC 948

HSP 3 Score: 840.1 bits (2169), Expect = 3.8e-240
Identity = 498/934 (53.32%), Postives = 619/934 (66.27%), Query Frame = 1

Query: 4   SSCSSNLKWSYDVFLSFRGEDTRNNFTSHLDRALREKGVNFFIDDKLERGGQISESLLKS 63
           SS S + KWSYDVFLSF+G+DTR+NFTSHLD ALR+KGVN FIDDKL+RG QISE+L K+
Sbjct: 10  SSTSLSFKWSYDVFLSFKGDDTRSNFTSHLDMALRQKGVNVFIDDKLKRGEQISETLFKA 69

Query: 64  IDGSKISIIIFSKNYASSTWCLDELVKIVQCMKSMGHIVFPVFYKVDPSEVRKQTGGFGE 123
           I  + ISI+IFS+NYASS+WCLDELVKI++C KS G +V P+FYKVDPS+VRKQTG FGE
Sbjct: 70  IQETLISIVIFSQNYASSSWCLDELVKIIECKKSKGQLVLPIFYKVDPSDVRKQTGCFGE 129

Query: 124 ALAKHEANELMTNKVQPWKEALTTAASLSGWDLATRKNEADLIHDLVKEVLSILNQTQ-L 183
           ALAKH+AN +   K Q W++ALTT A+ SGWDL TRK EAD I DLVKEVLS LN     
Sbjct: 130 ALAKHQANFM--EKTQIWRDALTTVANFSGWDLGTRK-EADFIQDLVKEVLSRLNCANGQ 189

Query: 184 LHVAKHPVGIDSQLRAVEELASH---DVPDGVNMVGIHGMGGIGKTTLAKALYNKIAYQF 243
           L+VAK+PVGIDSQL  ++ L SH   DV DGV M+GI+G+GGIGKTTLAKALYNKIA QF
Sbjct: 190 LYVAKYPVGIDSQLEDMK-LLSHQIRDVFDGVYMMGIYGIGGIGKTTLAKALYNKIANQF 249

Query: 244 EACCFLSNVRETLEQFKDLVQLQEKLLSEILKDNAWKVGNVHKGKNIIRDRLCSKKVLII 303
           E  CFLSNVRE  +QF  LVQLQEKLL EILK +  KV N+ +G NIIR RL SKKVLI+
Sbjct: 250 EGFCFLSNVREASKQFNGLVQLQEKLLYEILKVDL-KVDNLDEGINIIRSRLRSKKVLIV 309

Query: 304 LDDVDKDEQLDALVGERDWFGRGSKIIATTRDRHLLENHSFDIVYPIQLLDPKKSLELFS 363
           LDDVDK +QL+ALVG RDWFGRGSKII TTR+ HLL +H FD  Y I+ L    +LELFS
Sbjct: 310 LDDVDKLKQLEALVGGRDWFGRGSKIIVTTRNSHLLSSHEFDEKYGIRELSHGHALELFS 369

Query: 364 LHAFKQNHPSSNYVDLSKFAVSYCKGLPLALVILGSLLHKRERKIWKSKLHELENSLEPS 423
            HAFK++HPSSNY+DLS+ A SYCKG PLAL +LGS L  R++  WK+ L E ENSL   
Sbjct: 370 WHAFKKSHPSSNYLDLSERATSYCKGHPLALAVLGSFLCTRDQTKWKTILDEFENSLSED 429

Query: 424 VEAVFQIGFKELHERVKEIFLDISCFFVGEDINYSKDVLKACDLNPDYGIIILMDLSLVT 483
           +E + QI F  L E++KEIFLDISC FVGE +NY K VL  C  + D+GII+L+DLSL+T
Sbjct: 430 IEHIIQISFDGLEEKIKEIFLDISCLFVGEKVNYVKSVLNTCHFSLDFGIIVLIDLSLIT 489

Query: 484 VEDGKIQMHDLIQQMGQTIVRHESFEPAKRSRLWEAEGAIKILKEKSVSDFRQCFYLLIA 543
           VE+ ++QMHDLI+QMGQ IV  ESFEP KRSRLW     +K+  + S +   +   L ++
Sbjct: 490 VENEEVQMHDLIRQMGQKIVNGESFEPGKRSRLWLVHDVLKVFADNSGTIAVKAIKLDLS 549

Query: 544 K----DIYSEAFRNMKNLRLLILQRVAYFPRNIFEYLPNSLKWIE--------------- 603
                D+ S+AFRNMKNLRLLI+ R A F  N+ EYLP+SLKWI+               
Sbjct: 550 NPTRLDVDSQAFRNMKNLRLLIV-RNAKFSTNV-EYLPDSLKWIKWHGFSHRSLPLSFLK 609

Query: 604 --------WSTFYVN-----------QSSSISFS----ETPNFFATLNLEKLYLRGCTSL 663
                     +F  N           +   +S+S    + P+F AT NLE+LYL  CT+L
Sbjct: 610 KNLVGLDLSHSFIKNLGKGFKDCKRLKHGDLSYSSLLEKIPDFPATSNLEELYLNNCTNL 669

Query: 664 KGMRPS-----------------FRKFPSHLKFKSLKVLNLRDCLNLEEITDFSMASNLE 723
           + +  S                   K PS+L  KSLKVL L  C  LE++ DFS ASNLE
Sbjct: 670 RIIPKSVVSLGKLLTLDLDHCSNLIKLPSYLMLKSLKVLKLSYCKKLEKLPDFSTASNLE 729

Query: 724 ILDLNTCFSLRIIHESIGSLDKLITLQLDLCHNLEKLPSSLKLKSLDSLSFTNCYKLEQL 783
            L L  C +L++IH+SIG L KL+TL L  C NLEKLPS L LKSL+ L+  +C KLE++
Sbjct: 730 KLYLKECTNLKMIHDSIGCLSKLVTLDLGKCSNLEKLPSYLTLKSLEYLNLAHCKKLEEV 789

Query: 784 PEFDE--NMKSLRVMNLNGTAIRVLPSSIGYLIGLENLNLNDCANLTALPNEIHWLKSLE 843
           P+F    N+KSL +     T +RV+  SIG L  L  L+L  C NL  LP+ +  LKSL 
Sbjct: 790 PDFSSALNLKSLYLEQC--TNLRVIHESIGSLNSLVTLDLRQCTNLEKLPSYLK-LKSLT 849

Query: 844 ELHLRGCSKLDMFPPRSSLNFSQESSYFKLTVLDLKNCNISNSDFLETLSNV--CTSLEK 869
              L GC KL+MFP  +              +  L + ++ ++   E  S++   T+L  
Sbjct: 850 HFELSGCCKLEMFPKIAE------------NMKSLMSLHLDSTAIRELPSSIGFLTALLL 909