Cucsa.274720 (gene) Cucumber (Gy14) v1

NameCucsa.274720
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionNitrate reductase
Locationscaffold02541 : 55549 .. 59039 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTACCTCAATCAACAATCCCCAATTCTCTCATTTGGATTCTTTCTCTAACCTCCGCTCCGACTCATCACTTCGTTGTTGCAATTTCCCTTCCATTCCCACCAATCATCTCCTAATCAACAACCACGACGACAATGAAGATTTCTCTAGTGACGATGACAACGACAATGACTCTGATTTTACCCATTTGATTCGTAAAGGGATTCAAGAATTGGAACCCTCCATTTTGGACCCCAGAGATTTCGGAACGGCTGATTGTTGGATCCACCGAAATGAATCATTGGTTCGTCTTACCGGTAAACATCCCTTCAATTCCGAGCCGCCGTTATCTCGTTTGATGCACCATGGGTTCATCACTCCCGTCCCTCTCCATTACGTTCGTAACCACGGTCCGGTACCCAAGGCTAAGTGGGAGGATTGGACCGTTGAGGTTTGCGGTTTAGTAAAAAAACCAACCCGGTTCACTATGAATCAACTCGTAAGCGATTTCAGCAGCCGTGAGTTTCCTGTCACACTCGTCTGCGCCGGAAACCGCCGTAGGGAGCACAATATGGTTAAAAAAACCATTGGCTTTAACTGGGGTGCAGGCGGGACTTCCACCTCGGTCTGGCGAGGGGTTCCACTACGAGACGTTCTGAAACGGTGTGGTATCTTCAGTCGTAAAAAAGGAGCACTCAATGTCTGCTTTGAAGGAGCTGAATGTCTGCCCGGCGGCGGTGGGTCCAAGTACGGTACTAGTATCAGAAAGGAAATAGCCATGGACCCAGCTAGAGATATAATTTTAGCCTATATGCAAAACGGGGACGTACTGTCACCGGACCATGGTTTTCCGGTGCGGGTTATCATCCCCGGTTTCATCGGTGGAAGGATGGTGAAATGGGTAAAACGCATTATCGTAACAACACAAGAATCCGATAGCTATTACCATTATAGAGACAACAAAGTCCTCCCTTCCCATGTCGATGAAGAATTAGCAAACGCAGAAGGTATAAATAATTAAAATAAATCCCCATAAATCTAATACAAAACAACCCATTTTAATTTCAACCCAATAAGATCATAATCTAATTAATAATGTATTGTGGATTAACTTGTAGCTTGGTGGTATAAGCCGGAGTGTATTATTACTGAGTTGAACATAAACTCGGTGATAACGACCCCATGTCATGAGGAAATCTTGCCGATAAATTCATGGACGACACAACGGCCATACACGTTGAGGGGATATGCATATTCTGGTGAGTAAGTCATTTATTTTGGTGATATATATTGAGGTAGGTGAGTATTTTATTAACGAGGGGAATTGAATAAGTAACTTTATGAATAATTAATTAGGAGGAGGAAAGAAAGTGACACGGGTGGAGGTGACTTTAGATGGTGGTGAGTCGTGGCATGTTTGCGATTTGGACTGTCCTGAGAAAGCTACAAAATACGGCAAATATTGGTGTTGGTGTTTTTGGTCACTGGAAGTTGAAGTTCTTGACTTACTTGCTGCTAAAGAAATTGCAGTACGCGCTTGGGATGAAACTTTCAACACTCAACCTGAAAAGCTCATTTGGAATCTCATGGGAATGATGAACAATTGTTGGTTTCAAGTGAAAACCAACATGTGTAAACCGCATAAAGGAGAGATCGGAATCGTTTTTGAACACCCAACTGTACCCGGCAACCAATCCGGCGGATGGATGGGTAAAGAAAGACATCTCGAAATATCCACCGAATCCAATCAAACCATGAAGAAGAGTATTTCGTCTCCCTTCATCAACACAACCTCAAAGACTTACTCCATCTCTGACGTTCAAAAGCACAACTCAGACCAATCCGCCTGGATCGTCGTACACGGCCATGTTTACGACTGCACCCGTTTCCTCAAAGACCACCCTGGGGGTATGGACAGCATTCTCATCAATGCCGGTACCGATTGTACGGAGGAATTCGACGCCATTCACTCGGACAAAGCCAAAAAAATGCTAGAGGAATACAGAATCGGCGAGTTGATCACCACCGGCTACATATCGGATTCATCGCCTAACAACTCCATCCATGGGGATTCTTTTAGTTCTAATCCTCACTTGGCTCCAATAACAGAAACGAGGCGCGTGGCTTTAATCCCGCGTGAAAAAATTCGCTGCAAATTAGTTTCCAAAACGTCAATCTCACACGACGTCAGACGGTTCAGAATCGGCTTGCCTTTAGACGATCAAATCCTGGGATTACCTGTAGGGAAACACATATTCCTATGTGCAAATATAGACGGTAAGCTCTGTATGAGGGCCTACACACCAACCACCTGCATCAATCAAGTCGGCTATTTCGACTTAGTGGTCAAAATCTATTTCAAAAACGTGAACCCAAAATTTCCAAACGGCGGACTCATGTCTCAGCATTTGGAGTCTCTCCCACTTGGTTCCGAACTAGAAATCAAAGGTCCGTTAGGCCACATCGAATACACCGGAAACGGAAATTTCGTGGTGGACGGTAGAGTAAAGAAGGCAAAGAAGCTAGCAATGTTGGCCGGCGGAACGGGAATAACGCCGATATATCAAGTGGCGCAGGCGGTGCTGAAGGATCCTGAGGATGAAACAGAGATGTTTTTGGTGTATGCGAACAGAACGGAAAATGATATTCTGTTGAAGGATGAGTTGGATGAATGGAGTAAAAGGGATAAGAGGTTTAAAGTGTGGTACGTTGTGAATGAGAGTGTAAGGGAAGGGTGGGAATATAGTGTGGGAGTGGTGAGGGAGAGCATTATGAGGGAGCATCTTCCAGAAGCTTCGCCGGAAACAATGGCGTTGGCTTGTGGACCACCGCCAATGATTAAGTTTGCTGTGAAACCCAGTTTGGAGAAGATGAATTACGACACTACTAACTCGTTGTTGGTGTTTTAGCCAAACTGTGATGGGCTGTTTGGGCCTCATGTTATTCCATAATTCCCCTCTTACCACATAACACCACCCCCACAGTTTCTTCTTCAACGCATCCCCGTCGTAGTCGTCTCCATACTCCTTTCCGTAACCCTGTCTCCTTTCTTCTCCCATGGCTAACCCACTCTGCCAAAGCAACACGACACCAAGATCGTTTGGGGAGGTTGACGAACAACAAGTGGATGAAGGGGAGAAACAGGGCAGTTCAAGCTGTGTGGAGGTGTTTGAAGCCGCCATTGATGAGCTTCACAGGTCGTTTCGGGTTCTTAGAAGATTGAGTAGGAGAAATTTTGACGCACAAATGGGGATCTAACGATGTGGGTCAGCCCTGCACTCTCAGATGAAGATACTTGCATTGAAGGATTTGAAGGTGAAAGGGGAAAAGTAGTGAATTTGCTAAAGAATCAAGCGGTGAAAGTTGTTTTATCACGAGTAATGCACTGAAATTGAAAATATGTAAGGAGATTTGGAATTTCTCTTGAATTTGAGGTGGATTTACATGTTATAAGATTGTATAACTTGTAATTGTACTGATTGAAACAATTAAAATTACATACTATTTTTT

mRNA sequence

ATGGCTACCTCAATCAACAATCCCCAATTCTCTCATTTGGATTCTTTCTCTAACCTCCGCTCCGACTCATCACTTCGTTGTTGCAATTTCCCTTCCATTCCCACCAATCATCTCCTAATCAACAACCACGACGACAATGAAGATTTCTCTAGTGACGATGACAACGACAATGACTCTGATTTTACCCATTTGATTCGTAAAGGGATTCAAGAATTGGAACCCTCCATTTTGGACCCCAGAGATTTCGGAACGGCTGATTGTTGGATCCACCGAAATGAATCATTGGTTCGTCTTACCGGTAAACATCCCTTCAATTCCGAGCCGCCGTTATCTCGTTTGATGCACCATGGGTTCATCACTCCCGTCCCTCTCCATTACGTTCGTAACCACGGTCCGGTACCCAAGGCTAAGTGGGAGGATTGGACCGTTGAGGTTTGCGGTTTAGTAAAAAAACCAACCCGGTTCACTATGAATCAACTCGTAAGCGATTTCAGCAGCCGTGAGTTTCCTGTCACACTCGTCTGCGCCGGAAACCGCCGTAGGGAGCACAATATGGTTAAAAAAACCATTGGCTTTAACTGGGGTGCAGGCGGGACTTCCACCTCGGTCTGGCGAGGGGTTCCACTACGAGACGTTCTGAAACGGTGTGGTATCTTCAGTCGTAAAAAAGGAGCACTCAATGTCTGCTTTGAAGGAGCTGAATGTCTGCCCGGCGGCGGTGGGTCCAAGTACGGTACTAGTATCAGAAAGGAAATAGCCATGGACCCAGCTAGAGATATAATTTTAGCCTATATGCAAAACGGGGACGTACTGTCACCGGACCATGGTTTTCCGGTGCGGGTTATCATCCCCGGTTTCATCGGTGGAAGGATGGTGAAATGGGTAAAACGCATTATCGTAACAACACAAGAATCCGATAGCTATTACCATTATAGAGACAACAAAGTCCTCCCTTCCCATGTCGATGAAGAATTAGCAAACGCAGAAGCTTGGTGGTATAAGCCGGAGTGTATTATTACTGAGTTGAACATAAACTCGGTGATAACGACCCCATGTCATGAGGAAATCTTGCCGATAAATTCATGGACGACACAACGGCCATACACGTTGAGGGGATATGCATATTCTGGAGGAGGAAAGAAAGTGACACGGGTGGAGGTGACTTTAGATGGTGGTGAGTCGTGGCATGTTTGCGATTTGGACTGTCCTGAGAAAGCTACAAAATACGGCAAATATTGGTGTTGGTGTTTTTGGTCACTGGAAGTTGAAGTTCTTGACTTACTTGCTGCTAAAGAAATTGCAGTACGCGCTTGGGATGAAACTTTCAACACTCAACCTGAAAAGCTCATTTGGAATCTCATGGGAATGATGAACAATTGTTGGTTTCAAGTGAAAACCAACATGTGTAAACCGCATAAAGGAGAGATCGGAATCGTTTTTGAACACCCAACTGTACCCGGCAACCAATCCGGCGGATGGATGGGTAAAGAAAGACATCTCGAAATATCCACCGAATCCAATCAAACCATGAAGAAGAGTATTTCGTCTCCCTTCATCAACACAACCTCAAAGACTTACTCCATCTCTGACGTTCAAAAGCACAACTCAGACCAATCCGCCTGGATCGTCGTACACGGCCATGTTTACGACTGCACCCGTTTCCTCAAAGACCACCCTGGGGGTATGGACAGCATTCTCATCAATGCCGGTACCGATTGTACGGAGGAATTCGACGCCATTCACTCGGACAAAGCCAAAAAAATGCTAGAGGAATACAGAATCGGCGAGTTGATCACCACCGGCTACATATCGGATTCATCGCCTAACAACTCCATCCATGGGGATTCTTTTAGTTCTAATCCTCACTTGGCTCCAATAACAGAAACGAGGCGCGTGGCTTTAATCCCGCGTGAAAAAATTCGCTGCAAATTAGTTTCCAAAACGTCAATCTCACACGACGTCAGACGGTTCAGAATCGGCTTGCCTTTAGACGATCAAATCCTGGGATTACCTGTAGGGAAACACATATTCCTATGTGCAAATATAGACGGTAAGCTCTGTATGAGGGCCTACACACCAACCACCTGCATCAATCAAGTCGGCTATTTCGACTTAGTGGTCAAAATCTATTTCAAAAACGTGAACCCAAAATTTCCAAACGGCGGACTCATGTCTCAGCATTTGGAGTCTCTCCCACTTGGTTCCGAACTAGAAATCAAAGGTCCGTTAGGCCACATCGAATACACCGGAAACGGAAATTTCGTGGTGGACGGTAGAGTAAAGAAGGCAAAGAAGCTAGCAATGTTGGCCGGCGGAACGGGAATAACGCCGATATATCAAGTGGCGCAGGCGGTGCTGAAGGATCCTGAGGATGAAACAGAGATGTTTTTGGTGTATGCGAACAGAACGGAAAATGATATTCTGTTGAAGGATGAGTTGGATGAATGGAGTAAAAGGGATAAGAGGTTTAAAGTGTGGTACGTTGTGAATGAGAGTGTAAGGGAAGGGTGGGAATATAGTGTGGGAGTGGTGAGGGAGAGCATTATGAGGGAGCATCTTCCAGAAGCTTCGCCGGAAACAATGGCGTTGGCTTGTGGACCACCGCCAATGATTAAGTTTGCTGTGAAACCCAGTTTGGAGAAGATGAATTACGACACTACTAACTCGTTGTTGGTGTTTTAGCCAAACTGTGATGGGCTGTTTGGGCCTCATGTTATTCCATAATTCCCCTCTTACCACATAACACCACCCCCACAGTTTCTTCTTCAACGCATCCCCGTCGTAGTCGTCTCCATACTCCTTTCCGTAACCCTGTCTCCTTTCTTCTCCCATGGCTAACCCACTCTGCCAAAGCAACACGACACCAAGATCGTTTGGGGAGGTTGACGAACAACAAGTGGATGAAGGGGAGAAACAGGGCAGTTCAAGCTGTGTGGAGGTGTTTGAAGCCGCCATTGATGAGCTTCACAGGTCGTTTCGGGTTCTTAGAAGATTGAGTAGGAGAAATTTTGACGCACAAATGGGGATCTAACGATGTGGGTCAGCCCTGCACTCTCAGATGAAGATACTTGCATTGAAGGATTTGAAGGTGAAAGGGGAAAAGTAGTGAATTTGCTAAAGAATCAAGCGGTGAAAGTTGTTTTATCACGAGTAATGCACTGAAATTGAAAATATGTAAGGAGATTTGGAATTTCTCTTGAATTTGAGGTGGATTTACATGTTATAAGATTGTATAACTTGTAATTGTACTGATTGAAACAATTAAAATTACATACTATTTTTT

Coding sequence (CDS)

ATGGCTACCTCAATCAACAATCCCCAATTCTCTCATTTGGATTCTTTCTCTAACCTCCGCTCCGACTCATCACTTCGTTGTTGCAATTTCCCTTCCATTCCCACCAATCATCTCCTAATCAACAACCACGACGACAATGAAGATTTCTCTAGTGACGATGACAACGACAATGACTCTGATTTTACCCATTTGATTCGTAAAGGGATTCAAGAATTGGAACCCTCCATTTTGGACCCCAGAGATTTCGGAACGGCTGATTGTTGGATCCACCGAAATGAATCATTGGTTCGTCTTACCGGTAAACATCCCTTCAATTCCGAGCCGCCGTTATCTCGTTTGATGCACCATGGGTTCATCACTCCCGTCCCTCTCCATTACGTTCGTAACCACGGTCCGGTACCCAAGGCTAAGTGGGAGGATTGGACCGTTGAGGTTTGCGGTTTAGTAAAAAAACCAACCCGGTTCACTATGAATCAACTCGTAAGCGATTTCAGCAGCCGTGAGTTTCCTGTCACACTCGTCTGCGCCGGAAACCGCCGTAGGGAGCACAATATGGTTAAAAAAACCATTGGCTTTAACTGGGGTGCAGGCGGGACTTCCACCTCGGTCTGGCGAGGGGTTCCACTACGAGACGTTCTGAAACGGTGTGGTATCTTCAGTCGTAAAAAAGGAGCACTCAATGTCTGCTTTGAAGGAGCTGAATGTCTGCCCGGCGGCGGTGGGTCCAAGTACGGTACTAGTATCAGAAAGGAAATAGCCATGGACCCAGCTAGAGATATAATTTTAGCCTATATGCAAAACGGGGACGTACTGTCACCGGACCATGGTTTTCCGGTGCGGGTTATCATCCCCGGTTTCATCGGTGGAAGGATGGTGAAATGGGTAAAACGCATTATCGTAACAACACAAGAATCCGATAGCTATTACCATTATAGAGACAACAAAGTCCTCCCTTCCCATGTCGATGAAGAATTAGCAAACGCAGAAGCTTGGTGGTATAAGCCGGAGTGTATTATTACTGAGTTGAACATAAACTCGGTGATAACGACCCCATGTCATGAGGAAATCTTGCCGATAAATTCATGGACGACACAACGGCCATACACGTTGAGGGGATATGCATATTCTGGAGGAGGAAAGAAAGTGACACGGGTGGAGGTGACTTTAGATGGTGGTGAGTCGTGGCATGTTTGCGATTTGGACTGTCCTGAGAAAGCTACAAAATACGGCAAATATTGGTGTTGGTGTTTTTGGTCACTGGAAGTTGAAGTTCTTGACTTACTTGCTGCTAAAGAAATTGCAGTACGCGCTTGGGATGAAACTTTCAACACTCAACCTGAAAAGCTCATTTGGAATCTCATGGGAATGATGAACAATTGTTGGTTTCAAGTGAAAACCAACATGTGTAAACCGCATAAAGGAGAGATCGGAATCGTTTTTGAACACCCAACTGTACCCGGCAACCAATCCGGCGGATGGATGGGTAAAGAAAGACATCTCGAAATATCCACCGAATCCAATCAAACCATGAAGAAGAGTATTTCGTCTCCCTTCATCAACACAACCTCAAAGACTTACTCCATCTCTGACGTTCAAAAGCACAACTCAGACCAATCCGCCTGGATCGTCGTACACGGCCATGTTTACGACTGCACCCGTTTCCTCAAAGACCACCCTGGGGGTATGGACAGCATTCTCATCAATGCCGGTACCGATTGTACGGAGGAATTCGACGCCATTCACTCGGACAAAGCCAAAAAAATGCTAGAGGAATACAGAATCGGCGAGTTGATCACCACCGGCTACATATCGGATTCATCGCCTAACAACTCCATCCATGGGGATTCTTTTAGTTCTAATCCTCACTTGGCTCCAATAACAGAAACGAGGCGCGTGGCTTTAATCCCGCGTGAAAAAATTCGCTGCAAATTAGTTTCCAAAACGTCAATCTCACACGACGTCAGACGGTTCAGAATCGGCTTGCCTTTAGACGATCAAATCCTGGGATTACCTGTAGGGAAACACATATTCCTATGTGCAAATATAGACGGTAAGCTCTGTATGAGGGCCTACACACCAACCACCTGCATCAATCAAGTCGGCTATTTCGACTTAGTGGTCAAAATCTATTTCAAAAACGTGAACCCAAAATTTCCAAACGGCGGACTCATGTCTCAGCATTTGGAGTCTCTCCCACTTGGTTCCGAACTAGAAATCAAAGGTCCGTTAGGCCACATCGAATACACCGGAAACGGAAATTTCGTGGTGGACGGTAGAGTAAAGAAGGCAAAGAAGCTAGCAATGTTGGCCGGCGGAACGGGAATAACGCCGATATATCAAGTGGCGCAGGCGGTGCTGAAGGATCCTGAGGATGAAACAGAGATGTTTTTGGTGTATGCGAACAGAACGGAAAATGATATTCTGTTGAAGGATGAGTTGGATGAATGGAGTAAAAGGGATAAGAGGTTTAAAGTGTGGTACGTTGTGAATGAGAGTGTAAGGGAAGGGTGGGAATATAGTGTGGGAGTGGTGAGGGAGAGCATTATGAGGGAGCATCTTCCAGAAGCTTCGCCGGAAACAATGGCGTTGGCTTGTGGACCACCGCCAATGATTAAGTTTGCTGTGAAACCCAGTTTGGAGAAGATGAATTACGACACTACTAACTCGTTGTTGGTGTTTTAG

Protein sequence

MATSINNPQFSHLDSFSNLRSDSSLRCCNFPSIPTNHLLINNHDDNEDFSSDDDNDNDSDFTHLIRKGIQELEPSILDPRDFGTADCWIHRNESLVRLTGKHPFNSEPPLSRLMHHGFITPVPLHYVRNHGPVPKAKWEDWTVEVCGLVKKPTRFTMNQLVSDFSSREFPVTLVCAGNRRREHNMVKKTIGFNWGAGGTSTSVWRGVPLRDVLKRCGIFSRKKGALNVCFEGAECLPGGGGSKYGTSIRKEIAMDPARDIILAYMQNGDVLSPDHGFPVRVIIPGFIGGRMVKWVKRIIVTTQESDSYYHYRDNKVLPSHVDEELANAEAWWYKPECIITELNINSVITTPCHEEILPINSWTTQRPYTLRGYAYSGGGKKVTRVEVTLDGGESWHVCDLDCPEKATKYGKYWCWCFWSLEVEVLDLLAAKEIAVRAWDETFNTQPEKLIWNLMGMMNNCWFQVKTNMCKPHKGEIGIVFEHPTVPGNQSGGWMGKERHLEISTESNQTMKKSISSPFINTTSKTYSISDVQKHNSDQSAWIVVHGHVYDCTRFLKDHPGGMDSILINAGTDCTEEFDAIHSDKAKKMLEEYRIGELITTGYISDSSPNNSIHGDSFSSNPHLAPITETRRVALIPREKIRCKLVSKTSISHDVRRFRIGLPLDDQILGLPVGKHIFLCANIDGKLCMRAYTPTTCINQVGYFDLVVKIYFKNVNPKFPNGGLMSQHLESLPLGSELEIKGPLGHIEYTGNGNFVVDGRVKKAKKLAMLAGGTGITPIYQVAQAVLKDPEDETEMFLVYANRTENDILLKDELDEWSKRDKRFKVWYVVNESVREGWEYSVGVVRESIMREHLPEASPETMALACGPPPMIKFAVKPSLEKMNYDTTNSLLVF*
BLAST of Cucsa.274720 vs. Swiss-Prot
Match: NIA_CUCMA (Nitrate reductase [NADH] OS=Cucurbita maxima PE=2 SV=1)

HSP 1 Score: 1447.2 bits (3745), Expect = 0.0e+00
Identity = 696/925 (75.24%), Postives = 799/925 (86.38%), Query Frame = 1

Query: 1   MATSINNPQFSHLD--------SFSN---LRSDSSLRCCNFPSIPTNHLLINNH------ 60
           MA S++N Q+  L         SF N    R+DS +R CNFP+   ++   NN+      
Sbjct: 1   MAASVDNRQYGPLQPPLSGVVRSFKNGPNHRADSPVRGCNFPNSNVDY---NNNRPLKSS 60

Query: 61  --------DDNEDFSSDDDNDNDSDFTHLIRKGIQELEPSILDPRDFGTADCWIHRNESL 120
                   ++ ED  S+D+N+N+  F  LI KG +ELEPSILD RD GTAD WI RN S+
Sbjct: 61  VKIQEAAAEEMEDSCSEDENENE--FRDLIVKGNRELEPSILDHRDEGTADNWIERNASM 120

Query: 121 VRLTGKHPFNSEPPLSRLMHHGFITPVPLHYVRNHGPVPKAKWEDWTVEVCGLVKKPTRF 180
           VRLTGKHPFNSEPPL+RLMHHGFITPVPLHYVRNHG VPKAKW DWTVEVCGLVK+P +F
Sbjct: 121 VRLTGKHPFNSEPPLNRLMHHGFITPVPLHYVRNHGVVPKAKWADWTVEVCGLVKRPAKF 180

Query: 181 TMNQLVSDFSSREFPVTLVCAGNRRREHNMVKKTIGFNWGAGGTSTSVWRGVPLRDVLKR 240
           TM+QL+++F  REFP TLVCAGNRR+E NMVK++IGFNWGA G STSVWR VPL D+LKR
Sbjct: 181 TMDQLLNEFRFREFPATLVCAGNRRKEQNMVKQSIGFNWGAAGVSTSVWRRVPLCDLLKR 240

Query: 241 CGIFSRKKGALNVCFEGAECLPGGGGSKYGTSIRKEIAMDPARDIILAYMQNGDVLSPDH 300
           CGI SRKKGALNVCFEGAE LPGGGGSKYGTSI+KE+AMDPARDIILAYMQNG+ L+PDH
Sbjct: 241 CGILSRKKGALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLAPDH 300

Query: 301 GFPVRVIIPGFIGGRMVKWVKRIIVTTQESDSYYHYRDNKVLPSHVDEELANAEAWWYKP 360
           GFPVR+IIPGFIGGRMVKW+KRIIVTT+ES++YYH++DN+VLPSHVD ++ANAEAWWYKP
Sbjct: 301 GFPVRMIIPGFIGGRMVKWLKRIIVTTKESENYYHFKDNRVLPSHVDADVANAEAWWYKP 360

Query: 361 ECIITELNINSVITTPCHEEILPINSWTTQRPYTLRGYAYSGGGKKVTRVEVTLDGGESW 420
           E II ELNINSVITTPCHEEILPIN+WTTQRPYTLRGY+YSGGGKKVTRVEVT+D GE+W
Sbjct: 361 EHIINELNINSVITTPCHEEILPINAWTTQRPYTLRGYSYSGGGKKVTRVEVTMDSGETW 420

Query: 421 HVCDLDCPEKATKYGKYWCWCFWSLEVEVLDLLAAKEIAVRAWDETFNTQPEKLIWNLMG 480
            VC LD PEKA KYGKYWCWCFWSLEVEVLDLL+AKEIAVRAWDET NTQPEKLIWNLMG
Sbjct: 421 QVCTLDHPEKANKYGKYWCWCFWSLEVEVLDLLSAKEIAVRAWDETHNTQPEKLIWNLMG 480

Query: 481 MMNNCWFQVKTNMCKPHKGEIGIVFEHPTVPGNQSGGWMGKERHLEISTESNQTMKKSIS 540
           MMNNCWF+VKTNMCKPHKGEIGIVFEHPT PGNQSGGWM +ERHLEISTESNQT+KKS+S
Sbjct: 481 MMNNCWFRVKTNMCKPHKGEIGIVFEHPTQPGNQSGGWMDRERHLEISTESNQTLKKSVS 540

Query: 541 SPFINTTSKTYSISDVQKHNSDQSAWIVVHGHVYDCTRFLKDHPGGMDSILINAGTDCTE 600
           +PF+NT S TY++S+V+KHNS QSAWI+VHGHVYDCTRFLKDHPGG DSILINAGTDCTE
Sbjct: 541 TPFMNTASNTYTLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTE 600

Query: 601 EFDAIHSDKAKKMLEEYRIGELITTGYISDS---SPNNSIHGDSFSSNPHLAPITE---T 660
           EFDAIHSDKAKKMLE+YRIGELITTGY SDS   SPNNS HG S  S  HLAPI E   +
Sbjct: 601 EFDAIHSDKAKKMLEDYRIGELITTGYASDSSSNSPNNSTHGASNFS--HLAPIREAPVS 660

Query: 661 RRVALIPREKIRCKLVSKTSISHDVRRFRIGLP-LDDQILGLPVGKHIFLCANIDGKLCM 720
           RRVAL P EKI CKL+SKTSISHDVR FR  LP   DQ LGLPVGKHIF+CA +DGKLCM
Sbjct: 661 RRVALAPNEKIPCKLISKTSISHDVRVFRFALPGGQDQALGLPVGKHIFICATVDGKLCM 720

Query: 721 RAYTPTTCINQVGYFDLVVKIYFKNVNPKFPNGGLMSQHLESLPLGSELEIKGPLGHIEY 780
           RAYTPT+ I+++G+F+LVVK+YFK V+PKFPNGG+MSQ+L+S+ +GS L++KGPLGHIEY
Sbjct: 721 RAYTPTSSIDEMGFFELVVKVYFKGVHPKFPNGGIMSQYLDSMEVGSTLDVKGPLGHIEY 780

Query: 781 TGNGNFVVDGRVKKAKKLAMLAGGTGITPIYQVAQAVLKDPEDETEMFLVYANRTENDIL 840
           TG GNF+V G+ + A++LAMLAGGTGITPIYQV QA+LKDPEDETEM++VYANRTE+DIL
Sbjct: 781 TGRGNFMVHGKPRFARRLAMLAGGTGITPIYQVVQAILKDPEDETEMYVVYANRTEDDIL 840

Query: 841 LKDELDEWSKRDKRFKVWYVVNESVREGWEYSVGVVRESIMREHLPEASPETMALACGPP 894
           L+DELD W+K+++R KVWYVV ES+REGWEYSVG + E+I+REH+P A+ +T+ALACGPP
Sbjct: 841 LRDELDTWAKKNQRLKVWYVVQESIREGWEYSVGFITENILREHIPAAAEDTLALACGPP 900

BLAST of Cucsa.274720 vs. Swiss-Prot
Match: NIA_SOLLC (Nitrate reductase [NADH] OS=Solanum lycopersicum GN=NIA PE=3 SV=1)

HSP 1 Score: 1424.5 bits (3686), Expect = 0.0e+00
Identity = 680/913 (74.48%), Postives = 782/913 (85.65%), Query Frame = 1

Query: 1   MATSINNPQFSHLD-SFSNL------RSDSSLRCCNFPSIPTNHLLINNHDDNE----DF 60
           MA S+ N Q++HL+   S +      R DS +R CNFP   +NH L      N     D+
Sbjct: 1   MAASVENRQYTHLEPGLSGVGRTFKPRPDSPVRGCNFPP-SSNHELPFQKKQNPPIYLDY 60

Query: 61  SSDDDNDND---SDFTHLIRKGIQELEPSILDPRDFGTADCWIHRNESLVRLTGKHPFNS 120
           SS +D D+D   +++  +I+KG  ELEPSI D RD GTAD WI RN SL+RLTGKHPFNS
Sbjct: 61  SSSEDEDDDDEKNEYVQMIKKGKTELEPSIHDTRDEGTADNWIERNFSLIRLTGKHPFNS 120

Query: 121 EPPLSRLMHHGFITPVPLHYVRNHGPVPKAKWEDWTVEVCGLVKKPTRFTMNQLVSDFSS 180
           EPPLSRLMHHGFITPVPLHYVRNHGPVPKA W DWTVEV GLVK+P +FTM+QLV++F S
Sbjct: 121 EPPLSRLMHHGFITPVPLHYVRNHGPVPKASWSDWTVEVTGLVKRPMKFTMDQLVNEFPS 180

Query: 181 REFPVTLVCAGNRRREHNMVKKTIGFNWGAGGTSTSVWRGVPLRDVLKRCGIFSRKKGAL 240
           REFPVTLVCAGNRR+E NMVK+TIGFNWGA   ST+VWRGVPLR +LKRCG+ S+KKGAL
Sbjct: 181 REFPVTLVCAGNRRKEQNMVKQTIGFNWGAAAVSTTVWRGVPLRALLKRCGVQSKKKGAL 240

Query: 241 NVCFEGAECLPGGGGSKYGTSIRKEIAMDPARDIILAYMQNGDVLSPDHGFPVRVIIPGF 300
           NVCFEG++ LPGGGGSKYGTSI+KE AMDP+RDII+AYMQNG++LSPDHGFPVR+IIPGF
Sbjct: 241 NVCFEGSDVLPGGGGSKYGTSIKKEFAMDPSRDIIVAYMQNGEMLSPDHGFPVRMIIPGF 300

Query: 301 IGGRMVKWVKRIIVTTQESDSYYHYRDNKVLPSHVDEELANAEAWWYKPECIITELNINS 360
           IGGRMVKW+KRI+VTTQES+SYYHY+DN+VLP HVD ELANAEAWWYKPE II ELNINS
Sbjct: 301 IGGRMVKWLKRIVVTTQESESYYHYKDNRVLPPHVDAELANAEAWWYKPEYIINELNINS 360

Query: 361 VITTPCHEEILPINSWTTQRPYTLRGYAYSGGGKKVTRVEVTLDGGESWHVCDLDCPEKA 420
           VITTPCHEEILPIN+WTTQRPYTLRGYAYSGGGKKVTRVEVTLDGGE+W VC LD PEK 
Sbjct: 361 VITTPCHEEILPINAWTTQRPYTLRGYAYSGGGKKVTRVEVTLDGGETWSVCTLDHPEKP 420

Query: 421 TKYGKYWCWCFWSLEVEVLDLLAAKEIAVRAWDETFNTQPEKLIWNLMGMMNNCWFQVKT 480
           TKYGKYWCWCFWSLEVEVLDLL+AKEIAVRA DET NTQPEKLIWN+MGMMNNCWF+VK 
Sbjct: 421 TKYGKYWCWCFWSLEVEVLDLLSAKEIAVRATDETLNTQPEKLIWNVMGMMNNCWFRVKM 480

Query: 481 NMCKPHKGEIGIVFEHPTVPGNQSGGWMGKERHLEISTESNQTMKKSISSPFINTTSKTY 540
           N+CKPHKGEIGIVFEHPT PGNQSGGWM KERHLEIS  +  T+KKSIS+PF+NT SK Y
Sbjct: 481 NVCKPHKGEIGIVFEHPTQPGNQSGGWMAKERHLEISAVAPPTLKKSISTPFMNTASKMY 540

Query: 541 SISDVQKHNSDQSAWIVVHGHVYDCTRFLKDHPGGMDSILINAGTDCTEEFDAIHSDKAK 600
           S+S+V+KHNS  SAWI+VHGH+YD +RFLKDHPGG+DSILINAGTDCTEEFDAIHSDKAK
Sbjct: 541 SMSEVRKHNSSDSAWIIVHGHIYDASRFLKDHPGGVDSILINAGTDCTEEFDAIHSDKAK 600

Query: 601 KMLEEYRIGELITTGYISDSSPNNSIHGDSFSSNPHLAPI-----TETRRVALIPREKIR 660
           K+LE++RIGELITTGY SDSSPN+S+HG S S +  LAPI     T TR VALIPREKI 
Sbjct: 601 KLLEDFRIGELITTGYTSDSSPNSSVHGSS-SISSFLAPIKELVQTPTRSVALIPREKIP 660

Query: 661 CKLVSKTSISHDVRRFRIGLPLDDQILGLPVGKHIFLCANIDGKLCMRAYTPTTCINQVG 720
           CKLV K SISHDVR+F+  LP +DQ+LGLPVGKHIFLCA +D KLCMRAYTPT+ +++VG
Sbjct: 661 CKLVDKQSISHDVRKFKFALPSEDQVLGLPVGKHIFLCATVDDKLCMRAYTPTSTVDEVG 720

Query: 721 YFDLVVKIYFKNVNPKFPNGGLMSQHLESLPLGSELEIKGPLGHIEYTGNGNFVVDGRVK 780
           +F+LVVKIYFK V+PKFPNGG MSQHL+SLP+G+ L++KGPLGHIEY G GNF+V G+ K
Sbjct: 721 FFELVVKIYFKGVHPKFPNGGQMSQHLDSLPIGAFLDVKGPLGHIEYQGKGNFLVHGKQK 780

Query: 781 KAKKLAMLAGGTGITPIYQVAQAVLKDPEDETEMFLVYANRTENDILLKDELDEWSKR-D 840
            AKKLAM+AGGTGITP+YQV Q++LKDPED+TEM++VYANRTE+DILLKDELD W+++  
Sbjct: 781 FAKKLAMIAGGTGITPVYQVMQSILKDPEDDTEMYVVYANRTEDDILLKDELDAWAEQVP 840

Query: 841 KRFKVWYVVNESVREGWEYSVGVVRESIMREHLPEASPETMALACGPPPMIKFAVKPSLE 894
            R KVWYVV ES+ +GW+YS G V ESI+REH+PE S  T+ALACGPPPMI+FA+ P+LE
Sbjct: 841 NRVKVWYVVQESITQGWKYSTGFVTESILREHIPEPSHTTLALACGPPPMIQFAINPNLE 900

BLAST of Cucsa.274720 vs. Swiss-Prot
Match: NIA_PETHY (Nitrate reductase [NADH] OS=Petunia hybrida GN=NIA PE=2 SV=1)

HSP 1 Score: 1424.1 bits (3685), Expect = 0.0e+00
Identity = 683/911 (74.97%), Postives = 779/911 (85.51%), Query Frame = 1

Query: 1   MATSINNPQFSHLD--------SFSNLRSDSSLRCCNFP--SIPTNHLLINNHDDNEDFS 60
           MA S+ N QFSHL+        SF   RSDS +R CNFP  +  TN     N     D S
Sbjct: 1   MAASVENRQFSHLEPGLSGVVRSFKP-RSDSPVRGCNFPLNNELTNFQKKPNTTIYLDCS 60

Query: 61  SDDDNDNDSD---FTHLIRKGIQELEPSILDPRDFGTADCWIHRNESLVRLTGKHPFNSE 120
           S +D+D+D D   +  +IRKG  E+EPS+ D RD GTAD WI RN S++RLTGKHPFNSE
Sbjct: 61  SSEDDDDDDDKNEYLQMIRKGKLEVEPSVHDIRDEGTADNWIERNNSMIRLTGKHPFNSE 120

Query: 121 PPLSRLMHHGFITPVPLHYVRNHGPVPKAKWEDWTVEVCGLVKKPTRFTMNQLVSDFSSR 180
           PPL+RLMHHGFITPVPLHYVRNHGPVPK  W+DWTVEV GLVK+P +FTM QLV++F SR
Sbjct: 121 PPLARLMHHGFITPVPLHYVRNHGPVPKGMWDDWTVEVTGLVKRPMKFTMEQLVNEFPSR 180

Query: 181 EFPVTLVCAGNRRREHNMVKKTIGFNWGAGGTSTSVWRGVPLRDVLKRCGIFSRKKGALN 240
           E PVTLVCAGNRR+E NMVK+TIGFNWGA   ST+VWRGVPLR +LKRCGI+SR KGALN
Sbjct: 181 ELPVTLVCAGNRRKEQNMVKQTIGFNWGAAAVSTTVWRGVPLRAILKRCGIYSRTKGALN 240

Query: 241 VCFEGAECLPGGGGSKYGTSIRKEIAMDPARDIILAYMQNGDVLSPDHGFPVRVIIPGFI 300
           +CFEGA+ LPGGGGSKYGTSI+KE AMDP+RDII+AYMQNG+ L+PDHGFP+R+IIPGFI
Sbjct: 241 ICFEGADVLPGGGGSKYGTSIKKEFAMDPSRDIIIAYMQNGEKLTPDHGFPLRMIIPGFI 300

Query: 301 GGRMVKWVKRIIVTTQESDSYYHYRDNKVLPSHVDEELANAEAWWYKPECIITELNINSV 360
           GGRMVKW+KRIIVTTQES+SYYHY+DN+VLP HVD ELANAEAWWYKPE II ELNINSV
Sbjct: 301 GGRMVKWLKRIIVTTQESESYYHYKDNRVLPPHVDAELANAEAWWYKPEYIINELNINSV 360

Query: 361 ITTPCHEEILPINSWTTQRPYTLRGYAYSGGGKKVTRVEVTLDGGESWHVCDLDCPEKAT 420
           ITTPCHEEILPINSWTTQRPYTLRGY+YSGGGKKVTRVEVT+DGGE+W+VC +D PEK  
Sbjct: 361 ITTPCHEEILPINSWTTQRPYTLRGYSYSGGGKKVTRVEVTMDGGETWNVCTVDHPEKPN 420

Query: 421 KYGKYWCWCFWSLEVEVLDLLAAKEIAVRAWDETFNTQPEKLIWNLMGMMNNCWFQVKTN 480
           KYGKYWCWCFWSLEVEVLDLL+AKEIAVRAWDET NTQPEKLIWN+MGMMNNCWF+VKTN
Sbjct: 421 KYGKYWCWCFWSLEVEVLDLLSAKEIAVRAWDETLNTQPEKLIWNVMGMMNNCWFRVKTN 480

Query: 481 MCKPHKGEIGIVFEHPTVPGNQSGGWMGKERHLEISTESNQTMKKSISSPFINTTSKTYS 540
           +CKPHKGEIGIVFEHPT PGN SGGWM KERHLEIS E+  T+KKSIS+PF+NT SK YS
Sbjct: 481 VCKPHKGEIGIVFEHPTQPGNLSGGWMAKERHLEISAEAPPTLKKSISTPFMNTASKMYS 540

Query: 541 ISDVQKHNSDQSAWIVVHGHVYDCTRFLKDHPGGMDSILINAGTDCTEEFDAIHSDKAKK 600
           +S+V+KHNS  SAWI+VHGHVYD TRFLKDHPGG+DSILINAGTDCTEEFDAIHSDKAKK
Sbjct: 541 MSEVKKHNSADSAWIIVHGHVYDATRFLKDHPGGIDSILINAGTDCTEEFDAIHSDKAKK 600

Query: 601 MLEEYRIGELITTGYISDSSPNNSIHGDSFSSNPHLAPITE----TRRVALIPREKIRCK 660
           +LE++RIGELITTGY SDSSPNNS+HG S S +  LAPI E     R VALIPREKI CK
Sbjct: 601 LLEDFRIGELITTGYTSDSSPNNSVHGSS-SFSGFLAPIKELAPAVRSVALIPREKIPCK 660

Query: 661 LVSKTSISHDVRRFRIGLPLDDQILGLPVGKHIFLCANIDGKLCMRAYTPTTCINQVGYF 720
           LV K SISHDVR+FR  LP +DQ+LGLPVGKHIFLCA ID KLCMRAYTPT+ +++VGYF
Sbjct: 661 LVDKKSISHDVRKFRFALPSEDQVLGLPVGKHIFLCAIIDDKLCMRAYTPTSTVDEVGYF 720

Query: 721 DLVVKIYFKNVNPKFPNGGLMSQHLESLPLGSELEIKGPLGHIEYTGNGNFVVDGRVKKA 780
           +LVVKIYFK + PKFPNGG MSQ+L+SLPLG+ +++KGPLGHIEY G GNF+V G+ K A
Sbjct: 721 ELVVKIYFKGIVPKFPNGGQMSQYLDSLPLGAFVDVKGPLGHIEYQGRGNFLVHGKRKFA 780

Query: 781 KKLAMLAGGTGITPIYQVAQAVLKDPEDETEMFLVYANRTENDILLKDELDEWS-KRDKR 840
           KKLAMLAGGTGITP+YQV QA+LKDPEDETEM +VYANRTE+DILLKDELD W+ K  +R
Sbjct: 781 KKLAMLAGGTGITPVYQVMQAILKDPEDETEMHVVYANRTEDDILLKDELDSWAVKLPER 840

Query: 841 FKVWYVVNESVREGWEYSVGVVRESIMREHLPEASPETMALACGPPPMIKFAVKPSLEKM 894
            KVWYVV +S++EGW+YS G + E+++REH+P  S  T+ALACGPPPMI+FAV P+LEKM
Sbjct: 841 VKVWYVVQDSIKEGWKYSTGFITEAVLREHIPLPSQTTLALACGPPPMIQFAVNPNLEKM 900

BLAST of Cucsa.274720 vs. Swiss-Prot
Match: NIA2_TOBAC (Nitrate reductase [NADH] 2 OS=Nicotiana tabacum GN=NIA2 PE=2 SV=2)

HSP 1 Score: 1424.1 bits (3685), Expect = 0.0e+00
Identity = 678/906 (74.83%), Postives = 781/906 (86.20%), Query Frame = 1

Query: 1   MATSINNPQFSHLDSFSNL----RSDSSLRCCNFPSI-PTNHLLINNHDDNEDFSS---D 60
           MA S+ N QFSHL++  +     RSDS +R CNFPS   TN     N     D+SS   D
Sbjct: 1   MAASVENRQFSHLEAGLSRSFKPRSDSPVRGCNFPSPNSTNFQKKPNSTIYLDYSSSEDD 60

Query: 61  DDNDNDSDFTHLIRKGIQELEPSILDPRDFGTADCWIHRNESLVRLTGKHPFNSEPPLSR 120
           DD+D  +++  +I+KG  ELEPS+ D RD GTAD WI RN S++RLTGKHPFNSEPPL+R
Sbjct: 61  DDDDEKNEYLQMIKKGNSELEPSVHDTRDEGTADNWIERNFSMIRLTGKHPFNSEPPLNR 120

Query: 121 LMHHGFITPVPLHYVRNHGPVPKAKWEDWTVEVCGLVKKPTRFTMNQLVSDFSSREFPVT 180
           LMHHGFITPVPLHYVRNHGPVPK  W+DWTVEV GLVK+P +FTM+QLV++F  RE PVT
Sbjct: 121 LMHHGFITPVPLHYVRNHGPVPKGTWDDWTVEVTGLVKRPMKFTMDQLVNEFPCRELPVT 180

Query: 181 LVCAGNRRREHNMVKKTIGFNWGAGGTSTSVWRGVPLRDVLKRCGIFSRKKGALNVCFEG 240
           LVCAGNRR+E NMVK+TIGFNWGA   ST++WRGVPLR +LKRCG+FS+ KGALNVCFEG
Sbjct: 181 LVCAGNRRKEQNMVKQTIGFNWGAAAVSTTIWRGVPLRALLKRCGVFSKNKGALNVCFEG 240

Query: 241 AECLPGGGGSKYGTSIRKEIAMDPARDIILAYMQNGDVLSPDHGFPVRVIIPGFIGGRMV 300
           A+ LPGGGGSKYGTSI+KE AMDPARDII+AYMQNG+ L+PDHGFPVR+IIPGFIGGRMV
Sbjct: 241 ADVLPGGGGSKYGTSIKKEFAMDPARDIIVAYMQNGEKLAPDHGFPVRMIIPGFIGGRMV 300

Query: 301 KWVKRIIVTTQESDSYYHYRDNKVLPSHVDEELANAEAWWYKPECIITELNINSVITTPC 360
           KW+KRIIVTTQESDSYYH++DN+VLP HVD ELAN EAWWYKPE II ELNINSVITTPC
Sbjct: 301 KWIKRIIVTTQESDSYYHFKDNRVLPPHVDAELANTEAWWYKPEYIINELNINSVITTPC 360

Query: 361 HEEILPINSWTTQRPYTLRGYAYSGGGKKVTRVEVTLDGGESWHVCDLDCPEKATKYGKY 420
           HEEILPIN+WTTQRPYTLRGY+YSGGGKKVTRVEVTLDGGE+W V  LD PEK TKYGKY
Sbjct: 361 HEEILPINAWTTQRPYTLRGYSYSGGGKKVTRVEVTLDGGETWQVSTLDHPEKPTKYGKY 420

Query: 421 WCWCFWSLEVEVLDLLAAKEIAVRAWDETFNTQPEKLIWNLMGMMNNCWFQVKTNMCKPH 480
           WCWCFWSLEVEVLDLL+AKEIAVRAWDET NTQPEKLIWN+MGMMNNCWF+VK N+CKPH
Sbjct: 421 WCWCFWSLEVEVLDLLSAKEIAVRAWDETLNTQPEKLIWNVMGMMNNCWFRVKMNVCKPH 480

Query: 481 KGEIGIVFEHPTVPGNQSGGWMGKERHLEISTESNQTMKKSISSPFINTTSKTYSISDVQ 540
           KGEIGIVFEHPT PGNQSGGWM KERHLEIS E+ QT+KKSIS+PF+NT SK YS+S+V+
Sbjct: 481 KGEIGIVFEHPTQPGNQSGGWMAKERHLEISAEAPQTLKKSISTPFMNTASKMYSMSEVR 540

Query: 541 KHNSDQSAWIVVHGHVYDCTRFLKDHPGGMDSILINAGTDCTEEFDAIHSDKAKKMLEEY 600
           KH+S  SAWI+VHGH+YD TRFLKDHPGG DSILINAGTDCTEEFDAIHSDKAKK+LE++
Sbjct: 541 KHSSADSAWIIVHGHIYDATRFLKDHPGGTDSILINAGTDCTEEFDAIHSDKAKKLLEDF 600

Query: 601 RIGELITTGYISDSSPNNSIHGDSFSSNPHLAPITE----TRRVALIPREKIRCKLVSKT 660
           RIGELITTGY SD SP NS+HG S S +  LAPI E     R VALIPREKI CKL+ K 
Sbjct: 601 RIGELITTGYTSD-SPGNSVHGSS-SFSSFLAPIKELVPAQRSVALIPREKIPCKLIDKQ 660

Query: 661 SISHDVRRFRIGLPLDDQILGLPVGKHIFLCANIDGKLCMRAYTPTTCINQVGYFDLVVK 720
           SISHDVR+FR  LP +DQ+LGLPVGKHIFLCA ID KLCMRAYTPT+ I++VGYF+LVVK
Sbjct: 661 SISHDVRKFRFALPSEDQVLGLPVGKHIFLCAVIDDKLCMRAYTPTSTIDEVGYFELVVK 720

Query: 721 IYFKNVNPKFPNGGLMSQHLESLPLGSELEIKGPLGHIEYTGNGNFVVDGRVKKAKKLAM 780
           IYFK ++PKFPNGG MSQ+L+S+PLGS L++KGPLGHIEY G GNF+V G+ K AKKLAM
Sbjct: 721 IYFKGIHPKFPNGGQMSQYLDSMPLGSFLDVKGPLGHIEYQGKGNFLVHGKQKFAKKLAM 780

Query: 781 LAGGTGITPIYQVAQAVLKDPEDETEMFLVYANRTENDILLKDELDEWSKR-DKRFKVWY 840
           +AGGTGITP+YQV QA+LKDPED+TEM++VYANRTE+DILLK+ELD W+++  +R KVWY
Sbjct: 781 IAGGTGITPVYQVMQAILKDPEDDTEMYVVYANRTEDDILLKEELDSWAEKIPERVKVWY 840

Query: 841 VVNESVREGWEYSVGVVRESIMREHLPEASPETMALACGPPPMIKFAVKPSLEKMNYDTT 894
           VV +S++EGW+YS+G + E+I+REH+PE S  T+ALACGPPPMI+FAV P+LEKM YD  
Sbjct: 841 VVQDSIKEGWKYSIGFITEAILREHIPEPSHTTLALACGPPPMIQFAVNPNLEKMGYDIK 900

BLAST of Cucsa.274720 vs. Swiss-Prot
Match: NIA_BETPN (Nitrate reductase [NAD(P)H] OS=Betula pendula GN=NIA1 PE=2 SV=1)

HSP 1 Score: 1416.4 bits (3665), Expect = 0.0e+00
Identity = 674/908 (74.23%), Postives = 775/908 (85.35%), Query Frame = 1

Query: 1   MATSINNPQFSHLDSFSN------------LRSDSSLRCCNFPSIPTNHLLINNHDDNED 60
           MA S+ N +F+H +   N             RSDS       PS P   +        ED
Sbjct: 1   MAASVENRRFTHHEPAVNGLVRTFKPVPNSHRSDSPDLGRQIPSSPKKQVATG-----ED 60

Query: 61  FSSDDDNDNDSDFTHLIRKGIQELEPSILDPRDFGTADCWIHRNESLVRLTGKHPFNSEP 120
            SS+D+N+ND  +  LI+KG  ELEPSILDPRD  TAD W+ RN ++VRLTGKHPFNSE 
Sbjct: 61  SSSEDENEND--YKELIQKGNGELEPSILDPRDEATADNWVERNATMVRLTGKHPFNSEA 120

Query: 121 PLSRLMHHGFITPVPLHYVRNHGPVPKAKWEDWTVEVCGLVKKPTRFTMNQLVSDFSSRE 180
           PL+RLMHHGFITP PLHYVRNHGPVPKA+WEDW+VEVCGLVK+P RFTM++LV++F SRE
Sbjct: 121 PLTRLMHHGFITPAPLHYVRNHGPVPKARWEDWSVEVCGLVKRPARFTMDRLVTEFRSRE 180

Query: 181 FPVTLVCAGNRRREHNMVKKTIGFNWGAGGTSTSVWRGVPLRDVLKRCGIFSRKKGALNV 240
           FPVTLVCAGNRR+E NMVKKTIGFNWGA G STSVWRGVPLRDVLKRCGIFSR +GA NV
Sbjct: 181 FPVTLVCAGNRRKEQNMVKKTIGFNWGAAGVSTSVWRGVPLRDVLKRCGIFSRGRGAFNV 240

Query: 241 CFEGAECLPGGGGSKYGTSIRKEIAMDPARDIILAYMQNGDVLSPDHGFPVRVIIPGFIG 300
           CFEGAE LPGGGGSKYGTS++ E+AMDPARDIIL YMQNG+ LSPDHGFPVR+IIPGFIG
Sbjct: 241 CFEGAEDLPGGGGSKYGTSVKYEMAMDPARDIILGYMQNGERLSPDHGFPVRMIIPGFIG 300

Query: 301 GRMVKWVKRIIVTTQESDSYYHYRDNKVLPSHVDEELANAEAWWYKPECIITELNINSVI 360
           GRMVKW+KRIIVTT+ESD+YYHY DN+VLPSHVD ++A AEAWWYKPE II ELNINSVI
Sbjct: 301 GRMVKWLKRIIVTTKESDNYYHYNDNRVLPSHVDADVAKAEAWWYKPEHIINELNINSVI 360

Query: 361 TTPCHEEILPINSWTTQRPYTLRGYAYSGGGKKVTRVEVTLDGGESWHVCDLDCPEKATK 420
           TTPCHEEILPINSWTTQRPYTLRGYAYSGGG+KVTRVE+T++GGE W VC LD PEK  K
Sbjct: 361 TTPCHEEILPINSWTTQRPYTLRGYAYSGGGRKVTRVEITMNGGEKWRVCALDHPEKPNK 420

Query: 421 YGKYWCWCFWSLEVEVLDLLAAKEIAVRAWDETFNTQPEKLIWNLMGMMNNCWFQVKTNM 480
           YGKYWCWCFWSLEVEVLDLL AKEIAVRAWDE  NTQPEKLIWN+MGMMNNCWF+VKTN+
Sbjct: 421 YGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDEAHNTQPEKLIWNVMGMMNNCWFRVKTNV 480

Query: 481 CKPHKGEIGIVFEHPTVPGNQSGGWMGKERHLEISTESNQTMKKSISSPFINTTSKTYSI 540
           CK H GEIGI FEHPTVPGN+SGGWM +E++LE S+++NQ++KKS+SSPF+NT+SK +S+
Sbjct: 481 CKAHMGEIGIAFEHPTVPGNESGGWMAREKNLETSSDANQSLKKSVSSPFMNTSSKMFSM 540

Query: 541 SDVQKHNSDQSAWIVVHGHVYDCTRFLKDHPGGMDSILINAGTDCTEEFDAIHSDKAKKM 600
           S+V+KHNS +SAWI+VHGH+YDCT FLKDHPGG DSILINAGTDCTEEFDAIHSDKAKKM
Sbjct: 541 SEVKKHNSAESAWIIVHGHIYDCTHFLKDHPGGADSILINAGTDCTEEFDAIHSDKAKKM 600

Query: 601 LEEYRIGELITTGYISDSSPNNSIHGDSFSSNPHLAPITE---TRRVALIPREKIRCKLV 660
           LE+YRIGELITTGY+SD SPN+++HG S +S  HLAPI E    R VALIP  KI  KLV
Sbjct: 601 LEDYRIGELITTGYVSD-SPNSTVHGASNTS--HLAPIKEIAPLRNVALIPGAKIPTKLV 660

Query: 661 SKTSISHDVRRFRIGLPLDDQILGLPVGKHIFLCANIDGKLCMRAYTPTTCINQVGYFDL 720
            K S+SHDVR FR+ LP DDQ+LGLPVGKH+FLCA ID KLCMRAYTPT+ I++VGY DL
Sbjct: 661 YKKSLSHDVRLFRLALPSDDQVLGLPVGKHVFLCATIDDKLCMRAYTPTSTIDEVGYLDL 720

Query: 721 VVKIYFKNVNPKFPNGGLMSQHLESLPLGSELEIKGPLGHIEYTGNGNFVVDGRVKKAKK 780
           VVKIYFKN NP+FPNGGLMSQHL+SLP+GS L +KGPLGH+EYTG GNF+V G  K AK+
Sbjct: 721 VVKIYFKNSNPRFPNGGLMSQHLDSLPIGSVLHVKGPLGHVEYTGRGNFLVHGEPKFAKR 780

Query: 781 LAMLAGGTGITPIYQVAQAVLKDPEDETEMFLVYANRTENDILLKDELDEWSKRDKRFKV 840
           LAM+AGGTGITPIYQV QA+LKDPEDETEMF+VYANRTE+DILL++ELD+W+K+ ++ KV
Sbjct: 781 LAMVAGGTGITPIYQVIQAILKDPEDETEMFVVYANRTEDDILLREELDDWAKKHEKLKV 840

Query: 841 WYVVNESVREGWEYSVGVVRESIMREHLPEASPETMALACGPPPMIKFAVKPSLEKMNYD 894
           WYVV ES REGWEYSVG +RESI+REH+PE S + +ALACG P MI+ AV+ +LEKMNYD
Sbjct: 841 WYVVKESKREGWEYSVGYIRESILREHIPEGSDDVLALACGAPSMIEEAVRLNLEKMNYD 898

BLAST of Cucsa.274720 vs. TrEMBL
Match: A0A0A0L1Y7_CUCSA (Nitrate reductase OS=Cucumis sativus GN=Csa_4G377160 PE=3 SV=1)

HSP 1 Score: 1868.6 bits (4839), Expect = 0.0e+00
Identity = 893/893 (100.00%), Postives = 893/893 (100.00%), Query Frame = 1

Query: 1   MATSINNPQFSHLDSFSNLRSDSSLRCCNFPSIPTNHLLINNHDDNEDFSSDDDNDNDSD 60
           MATSINNPQFSHLDSFSNLRSDSSLRCCNFPSIPTNHLLINNHDDNEDFSSDDDNDNDSD
Sbjct: 1   MATSINNPQFSHLDSFSNLRSDSSLRCCNFPSIPTNHLLINNHDDNEDFSSDDDNDNDSD 60

Query: 61  FTHLIRKGIQELEPSILDPRDFGTADCWIHRNESLVRLTGKHPFNSEPPLSRLMHHGFIT 120
           FTHLIRKGIQELEPSILDPRDFGTADCWIHRNESLVRLTGKHPFNSEPPLSRLMHHGFIT
Sbjct: 61  FTHLIRKGIQELEPSILDPRDFGTADCWIHRNESLVRLTGKHPFNSEPPLSRLMHHGFIT 120

Query: 121 PVPLHYVRNHGPVPKAKWEDWTVEVCGLVKKPTRFTMNQLVSDFSSREFPVTLVCAGNRR 180
           PVPLHYVRNHGPVPKAKWEDWTVEVCGLVKKPTRFTMNQLVSDFSSREFPVTLVCAGNRR
Sbjct: 121 PVPLHYVRNHGPVPKAKWEDWTVEVCGLVKKPTRFTMNQLVSDFSSREFPVTLVCAGNRR 180

Query: 181 REHNMVKKTIGFNWGAGGTSTSVWRGVPLRDVLKRCGIFSRKKGALNVCFEGAECLPGGG 240
           REHNMVKKTIGFNWGAGGTSTSVWRGVPLRDVLKRCGIFSRKKGALNVCFEGAECLPGGG
Sbjct: 181 REHNMVKKTIGFNWGAGGTSTSVWRGVPLRDVLKRCGIFSRKKGALNVCFEGAECLPGGG 240

Query: 241 GSKYGTSIRKEIAMDPARDIILAYMQNGDVLSPDHGFPVRVIIPGFIGGRMVKWVKRIIV 300
           GSKYGTSIRKEIAMDPARDIILAYMQNGDVLSPDHGFPVRVIIPGFIGGRMVKWVKRIIV
Sbjct: 241 GSKYGTSIRKEIAMDPARDIILAYMQNGDVLSPDHGFPVRVIIPGFIGGRMVKWVKRIIV 300

Query: 301 TTQESDSYYHYRDNKVLPSHVDEELANAEAWWYKPECIITELNINSVITTPCHEEILPIN 360
           TTQESDSYYHYRDNKVLPSHVDEELANAEAWWYKPECIITELNINSVITTPCHEEILPIN
Sbjct: 301 TTQESDSYYHYRDNKVLPSHVDEELANAEAWWYKPECIITELNINSVITTPCHEEILPIN 360

Query: 361 SWTTQRPYTLRGYAYSGGGKKVTRVEVTLDGGESWHVCDLDCPEKATKYGKYWCWCFWSL 420
           SWTTQRPYTLRGYAYSGGGKKVTRVEVTLDGGESWHVCDLDCPEKATKYGKYWCWCFWSL
Sbjct: 361 SWTTQRPYTLRGYAYSGGGKKVTRVEVTLDGGESWHVCDLDCPEKATKYGKYWCWCFWSL 420

Query: 421 EVEVLDLLAAKEIAVRAWDETFNTQPEKLIWNLMGMMNNCWFQVKTNMCKPHKGEIGIVF 480
           EVEVLDLLAAKEIAVRAWDETFNTQPEKLIWNLMGMMNNCWFQVKTNMCKPHKGEIGIVF
Sbjct: 421 EVEVLDLLAAKEIAVRAWDETFNTQPEKLIWNLMGMMNNCWFQVKTNMCKPHKGEIGIVF 480

Query: 481 EHPTVPGNQSGGWMGKERHLEISTESNQTMKKSISSPFINTTSKTYSISDVQKHNSDQSA 540
           EHPTVPGNQSGGWMGKERHLEISTESNQTMKKSISSPFINTTSKTYSISDVQKHNSDQSA
Sbjct: 481 EHPTVPGNQSGGWMGKERHLEISTESNQTMKKSISSPFINTTSKTYSISDVQKHNSDQSA 540

Query: 541 WIVVHGHVYDCTRFLKDHPGGMDSILINAGTDCTEEFDAIHSDKAKKMLEEYRIGELITT 600
           WIVVHGHVYDCTRFLKDHPGGMDSILINAGTDCTEEFDAIHSDKAKKMLEEYRIGELITT
Sbjct: 541 WIVVHGHVYDCTRFLKDHPGGMDSILINAGTDCTEEFDAIHSDKAKKMLEEYRIGELITT 600

Query: 601 GYISDSSPNNSIHGDSFSSNPHLAPITETRRVALIPREKIRCKLVSKTSISHDVRRFRIG 660
           GYISDSSPNNSIHGDSFSSNPHLAPITETRRVALIPREKIRCKLVSKTSISHDVRRFRIG
Sbjct: 601 GYISDSSPNNSIHGDSFSSNPHLAPITETRRVALIPREKIRCKLVSKTSISHDVRRFRIG 660

Query: 661 LPLDDQILGLPVGKHIFLCANIDGKLCMRAYTPTTCINQVGYFDLVVKIYFKNVNPKFPN 720
           LPLDDQILGLPVGKHIFLCANIDGKLCMRAYTPTTCINQVGYFDLVVKIYFKNVNPKFPN
Sbjct: 661 LPLDDQILGLPVGKHIFLCANIDGKLCMRAYTPTTCINQVGYFDLVVKIYFKNVNPKFPN 720

Query: 721 GGLMSQHLESLPLGSELEIKGPLGHIEYTGNGNFVVDGRVKKAKKLAMLAGGTGITPIYQ 780
           GGLMSQHLESLPLGSELEIKGPLGHIEYTGNGNFVVDGRVKKAKKLAMLAGGTGITPIYQ
Sbjct: 721 GGLMSQHLESLPLGSELEIKGPLGHIEYTGNGNFVVDGRVKKAKKLAMLAGGTGITPIYQ 780

Query: 781 VAQAVLKDPEDETEMFLVYANRTENDILLKDELDEWSKRDKRFKVWYVVNESVREGWEYS 840
           VAQAVLKDPEDETEMFLVYANRTENDILLKDELDEWSKRDKRFKVWYVVNESVREGWEYS
Sbjct: 781 VAQAVLKDPEDETEMFLVYANRTENDILLKDELDEWSKRDKRFKVWYVVNESVREGWEYS 840

Query: 841 VGVVRESIMREHLPEASPETMALACGPPPMIKFAVKPSLEKMNYDTTNSLLVF 894
           VGVVRESIMREHLPEASPETMALACGPPPMIKFAVKPSLEKMNYDTTNSLLVF
Sbjct: 841 VGVVRESIMREHLPEASPETMALACGPPPMIKFAVKPSLEKMNYDTTNSLLVF 893

BLAST of Cucsa.274720 vs. TrEMBL
Match: E2IZV4_CUCSA (Nitrate reductase OS=Cucumis sativus GN=NR1 PE=2 SV=1)

HSP 1 Score: 1837.8 bits (4759), Expect = 0.0e+00
Identity = 877/877 (100.00%), Postives = 877/877 (100.00%), Query Frame = 1

Query: 1   MATSINNPQFSHLDSFSNLRSDSSLRCCNFPSIPTNHLLINNHDDNEDFSSDDDNDNDSD 60
           MATSINNPQFSHLDSFSNLRSDSSLRCCNFPSIPTNHLLINNHDDNEDFSSDDDNDNDSD
Sbjct: 1   MATSINNPQFSHLDSFSNLRSDSSLRCCNFPSIPTNHLLINNHDDNEDFSSDDDNDNDSD 60

Query: 61  FTHLIRKGIQELEPSILDPRDFGTADCWIHRNESLVRLTGKHPFNSEPPLSRLMHHGFIT 120
           FTHLIRKGIQELEPSILDPRDFGTADCWIHRNESLVRLTGKHPFNSEPPLSRLMHHGFIT
Sbjct: 61  FTHLIRKGIQELEPSILDPRDFGTADCWIHRNESLVRLTGKHPFNSEPPLSRLMHHGFIT 120

Query: 121 PVPLHYVRNHGPVPKAKWEDWTVEVCGLVKKPTRFTMNQLVSDFSSREFPVTLVCAGNRR 180
           PVPLHYVRNHGPVPKAKWEDWTVEVCGLVKKPTRFTMNQLVSDFSSREFPVTLVCAGNRR
Sbjct: 121 PVPLHYVRNHGPVPKAKWEDWTVEVCGLVKKPTRFTMNQLVSDFSSREFPVTLVCAGNRR 180

Query: 181 REHNMVKKTIGFNWGAGGTSTSVWRGVPLRDVLKRCGIFSRKKGALNVCFEGAECLPGGG 240
           REHNMVKKTIGFNWGAGGTSTSVWRGVPLRDVLKRCGIFSRKKGALNVCFEGAECLPGGG
Sbjct: 181 REHNMVKKTIGFNWGAGGTSTSVWRGVPLRDVLKRCGIFSRKKGALNVCFEGAECLPGGG 240

Query: 241 GSKYGTSIRKEIAMDPARDIILAYMQNGDVLSPDHGFPVRVIIPGFIGGRMVKWVKRIIV 300
           GSKYGTSIRKEIAMDPARDIILAYMQNGDVLSPDHGFPVRVIIPGFIGGRMVKWVKRIIV
Sbjct: 241 GSKYGTSIRKEIAMDPARDIILAYMQNGDVLSPDHGFPVRVIIPGFIGGRMVKWVKRIIV 300

Query: 301 TTQESDSYYHYRDNKVLPSHVDEELANAEAWWYKPECIITELNINSVITTPCHEEILPIN 360
           TTQESDSYYHYRDNKVLPSHVDEELANAEAWWYKPECIITELNINSVITTPCHEEILPIN
Sbjct: 301 TTQESDSYYHYRDNKVLPSHVDEELANAEAWWYKPECIITELNINSVITTPCHEEILPIN 360

Query: 361 SWTTQRPYTLRGYAYSGGGKKVTRVEVTLDGGESWHVCDLDCPEKATKYGKYWCWCFWSL 420
           SWTTQRPYTLRGYAYSGGGKKVTRVEVTLDGGESWHVCDLDCPEKATKYGKYWCWCFWSL
Sbjct: 361 SWTTQRPYTLRGYAYSGGGKKVTRVEVTLDGGESWHVCDLDCPEKATKYGKYWCWCFWSL 420

Query: 421 EVEVLDLLAAKEIAVRAWDETFNTQPEKLIWNLMGMMNNCWFQVKTNMCKPHKGEIGIVF 480
           EVEVLDLLAAKEIAVRAWDETFNTQPEKLIWNLMGMMNNCWFQVKTNMCKPHKGEIGIVF
Sbjct: 421 EVEVLDLLAAKEIAVRAWDETFNTQPEKLIWNLMGMMNNCWFQVKTNMCKPHKGEIGIVF 480

Query: 481 EHPTVPGNQSGGWMGKERHLEISTESNQTMKKSISSPFINTTSKTYSISDVQKHNSDQSA 540
           EHPTVPGNQSGGWMGKERHLEISTESNQTMKKSISSPFINTTSKTYSISDVQKHNSDQSA
Sbjct: 481 EHPTVPGNQSGGWMGKERHLEISTESNQTMKKSISSPFINTTSKTYSISDVQKHNSDQSA 540

Query: 541 WIVVHGHVYDCTRFLKDHPGGMDSILINAGTDCTEEFDAIHSDKAKKMLEEYRIGELITT 600
           WIVVHGHVYDCTRFLKDHPGGMDSILINAGTDCTEEFDAIHSDKAKKMLEEYRIGELITT
Sbjct: 541 WIVVHGHVYDCTRFLKDHPGGMDSILINAGTDCTEEFDAIHSDKAKKMLEEYRIGELITT 600

Query: 601 GYISDSSPNNSIHGDSFSSNPHLAPITETRRVALIPREKIRCKLVSKTSISHDVRRFRIG 660
           GYISDSSPNNSIHGDSFSSNPHLAPITETRRVALIPREKIRCKLVSKTSISHDVRRFRIG
Sbjct: 601 GYISDSSPNNSIHGDSFSSNPHLAPITETRRVALIPREKIRCKLVSKTSISHDVRRFRIG 660

Query: 661 LPLDDQILGLPVGKHIFLCANIDGKLCMRAYTPTTCINQVGYFDLVVKIYFKNVNPKFPN 720
           LPLDDQILGLPVGKHIFLCANIDGKLCMRAYTPTTCINQVGYFDLVVKIYFKNVNPKFPN
Sbjct: 661 LPLDDQILGLPVGKHIFLCANIDGKLCMRAYTPTTCINQVGYFDLVVKIYFKNVNPKFPN 720

Query: 721 GGLMSQHLESLPLGSELEIKGPLGHIEYTGNGNFVVDGRVKKAKKLAMLAGGTGITPIYQ 780
           GGLMSQHLESLPLGSELEIKGPLGHIEYTGNGNFVVDGRVKKAKKLAMLAGGTGITPIYQ
Sbjct: 721 GGLMSQHLESLPLGSELEIKGPLGHIEYTGNGNFVVDGRVKKAKKLAMLAGGTGITPIYQ 780

Query: 781 VAQAVLKDPEDETEMFLVYANRTENDILLKDELDEWSKRDKRFKVWYVVNESVREGWEYS 840
           VAQAVLKDPEDETEMFLVYANRTENDILLKDELDEWSKRDKRFKVWYVVNESVREGWEYS
Sbjct: 781 VAQAVLKDPEDETEMFLVYANRTENDILLKDELDEWSKRDKRFKVWYVVNESVREGWEYS 840

Query: 841 VGVVRESIMREHLPEASPETMALACGPPPMIKFAVKP 878
           VGVVRESIMREHLPEASPETMALACGPPPMIKFAVKP
Sbjct: 841 VGVVRESIMREHLPEASPETMALACGPPPMIKFAVKP 877

BLAST of Cucsa.274720 vs. TrEMBL
Match: E2IZV5_CUCSA (Nitrate reductase OS=Cucumis sativus GN=NR2 PE=2 SV=1)

HSP 1 Score: 1473.8 bits (3814), Expect = 0.0e+00
Identity = 700/918 (76.25%), Postives = 798/918 (86.93%), Query Frame = 1

Query: 1   MATSINNPQFSHLD-----------SFSNLRSDSSLRCCNFPSIPTNHLLINN------H 60
           MA S++N QF  L            S  N RSDS +R CNFP+   N+ L+ +       
Sbjct: 1   MAASVDNRQFGSLQPPLNGVVRSFKSGPNHRSDSPVRGCNFPNSNINNRLLKSSVKMEQE 60

Query: 61  DDNEDFSSDDDNDNDSDFTHLIRKGIQELEPSILDPRDFGTADCWIHRNESLVRLTGKHP 120
           ++++D  S+D+NDN+ +   LI+KG +ELEPS +DPRD GTAD WI RN S+VRLTGKHP
Sbjct: 61  EEDDDSCSEDENDNN-ELRELIKKGNRELEPSTVDPRDEGTADNWIERNSSMVRLTGKHP 120

Query: 121 FNSEPPLSRLMHHGFITPVPLHYVRNHGPVPKAKWEDWTVEVCGLVKKPTRFTMNQLVSD 180
           FNSEPPL+RLMHHGFITPVPLHYVRNHG VPKAKW DWT+EVCGLVK+PT+F+M+QLV++
Sbjct: 121 FNSEPPLNRLMHHGFITPVPLHYVRNHGAVPKAKWSDWTIEVCGLVKRPTKFSMDQLVNE 180

Query: 181 FSSREFPVTLVCAGNRRREHNMVKKTIGFNWGAGGTSTSVWRGVPLRDVLKRCGIFSRKK 240
           F  REFP TLVCAGNRR+E NMVKK+IGFNWGA G STSVWRGVPLR+VLKRCGI SRKK
Sbjct: 181 FRFREFPATLVCAGNRRKEQNMVKKSIGFNWGAAGVSTSVWRGVPLREVLKRCGIMSRKK 240

Query: 241 GALNVCFEGAECLPGGGGSKYGTSIRKEIAMDPARDIILAYMQNGDVLSPDHGFPVRVII 300
           GALNVCFEGAE LPGGGGSKYGTSI+KE+AMDPARDIILAYMQNG+ L+PDHGFPVR+II
Sbjct: 241 GALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLAPDHGFPVRMII 300

Query: 301 PGFIGGRMVKWVKRIIVTTQESDSYYHYRDNKVLPSHVDEELANAEAWWYKPECIITELN 360
           PGFIGGRMVKW+KRIIVTT+ES++YYH++DN+VLPSHVD ELANAEAWWYKPE II ELN
Sbjct: 301 PGFIGGRMVKWLKRIIVTTKESENYYHFKDNRVLPSHVDAELANAEAWWYKPEYIINELN 360

Query: 361 INSVITTPCHEEILPINSWTTQRPYTLRGYAYSGGGKKVTRVEVTLDGGESWHVCDLDCP 420
           INSVITTPCHEEILPINSWTTQRPYTLRGY+YSGGGKKVTRVEVT+DGGE+W VC LD P
Sbjct: 361 INSVITTPCHEEILPINSWTTQRPYTLRGYSYSGGGKKVTRVEVTMDGGETWQVCTLDHP 420

Query: 421 EKATKYGKYWCWCFWSLEVEVLDLLAAKEIAVRAWDETFNTQPEKLIWNLMGMMNNCWFQ 480
           EK  KYGKYWCWCFWSLEVEVLDLL AKEIAVRAWDET NTQPEKLIWNLMGMMNNCWF 
Sbjct: 421 EKPNKYGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNCWFT 480

Query: 481 VKTNMCKPHKGEIGIVFEHPTVPGNQSGGWMGKERHLEISTESNQTMKKSISSPFINTTS 540
           VKTN+CKPHKGEIGI+FEHPTVPGNQSGGWM +ERHLEISTESNQT+KKSIS+PF+NT S
Sbjct: 481 VKTNVCKPHKGEIGIIFEHPTVPGNQSGGWMDRERHLEISTESNQTLKKSISTPFMNTAS 540

Query: 541 KTYSISDVQKHNSDQSAWIVVHGHVYDCTRFLKDHPGGMDSILINAGTDCTEEFDAIHSD 600
            TYS+S+V+KHNS QSAWI+VHGHVYDCTRFLKDHPGG DSILINAGTDCTEEFDAIHSD
Sbjct: 541 NTYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAIHSD 600

Query: 601 KAKKMLEEYRIGELITTGYISDS---SPNNSIHGDSFSSNPHLAPITE----TRRVALIP 660
           KAKKMLE+YRIGELITTGY SDS   SPNNS HG S  S  HLAPI E    TRR AL P
Sbjct: 601 KAKKMLEDYRIGELITTGYASDSSSNSPNNSTHGASNFS--HLAPIREAPMVTRRAALAP 660

Query: 661 REKIRCKLVSKTSISHDVRRFRIGLP-LDDQILGLPVGKHIFLCANIDGKLCMRAYTPTT 720
            EKI CKL+SKT ISHDVR FR  LP   DQ+LGLPVGKHIF+CA +DGKLCMRAYTP++
Sbjct: 661 NEKIPCKLISKTEISHDVRVFRFELPGGQDQVLGLPVGKHIFICAKVDGKLCMRAYTPSS 720

Query: 721 CINQVGYFDLVVKIYFKNVNPKFPNGGLMSQHLESLPLGSELEIKGPLGHIEYTGNGNFV 780
            ++Q+GYF+LVVK+YFKNV+PKFPNGG+MSQ L+++ +GS +E+KGPLGHIEYTG GNF 
Sbjct: 721 TVDQMGYFELVVKVYFKNVHPKFPNGGIMSQFLDNMEVGSTVEVKGPLGHIEYTGRGNFT 780

Query: 781 VDGRVKKAKKLAMLAGGTGITPIYQVAQAVLKDPEDETEMFLVYANRTENDILLKDELDE 840
           V G+ + AK+LAMLAGGTGITPIYQ+ QA+LKDPEDETEMF+VYANRTE+DILL++ELD 
Sbjct: 781 VHGKPRFAKRLAMLAGGTGITPIYQIVQAILKDPEDETEMFVVYANRTEDDILLREELDT 840

Query: 841 WSKRDKRFKVWYVVNESVREGWEYSVGVVRESIMREHLPEASPETMALACGPPPMIKFAV 894
           W+K+++R KVWYVV ES+REGWEYS+G + E I REHLP A+ +T+AL CGPPPMI+FAV
Sbjct: 841 WAKKNERLKVWYVVQESIREGWEYSIGFITEEITREHLPAAAEDTLALVCGPPPMIQFAV 900

BLAST of Cucsa.274720 vs. TrEMBL
Match: E7BXW7_CUCSA (Nitrate reductase OS=Cucumis sativus PE=2 SV=1)

HSP 1 Score: 1468.0 bits (3799), Expect = 0.0e+00
Identity = 699/918 (76.14%), Postives = 797/918 (86.82%), Query Frame = 1

Query: 1   MATSINNPQFSHLD-----------SFSNLRSDSSLRCCNFPSIPTNHLLINN------H 60
           MA S++N QF  L            S  N RSDS +R CNFP+   N+ L+ +       
Sbjct: 1   MAASVDNRQFGSLQPPLNGVVRSFKSGPNHRSDSPVRGCNFPNSNINNRLLKSSVKMEQE 60

Query: 61  DDNEDFSSDDDNDNDSDFTHLIRKGIQELEPSILDPRDFGTADCWIHRNESLVRLTGKHP 120
           ++++D  S+D+NDN+ +   LI+KG +ELEPS +DPRD GTAD WI RN S+VRLTGKHP
Sbjct: 61  EEDDDSCSEDENDNN-ELRELIKKGNRELEPSTVDPRDEGTADNWIERNSSMVRLTGKHP 120

Query: 121 FNSEPPLSRLMHHGFITPVPLHYVRNHGPVPKAKWEDWTVEVCGLVKKPTRFTMNQLVSD 180
           FNSEPPL+RLMHHGFITPVPLHYVRNHG VPKAKW DWT+EVCGLVK+PT+F+M+QLV++
Sbjct: 121 FNSEPPLNRLMHHGFITPVPLHYVRNHGAVPKAKWADWTIEVCGLVKRPTKFSMDQLVNE 180

Query: 181 FSSREFPVTLVCAGNRRREHNMVKKTIGFNWGAGGTSTSVWRGVPLRDVLKRCGIFSRKK 240
           F  REFP TLVCAGNRR+E NMVKK+IGFNWGA G STSVWRGVPLR+VLKRCGI SRKK
Sbjct: 181 FRFREFPATLVCAGNRRKEQNMVKKSIGFNWGAAGVSTSVWRGVPLREVLKRCGIMSRKK 240

Query: 241 GALNVCFEGAECLPGGGGSKYGTSIRKEIAMDPARDIILAYMQNGDVLSPDHGFPVRVII 300
           GALNVCFEGAE LPGGGGSKYGTSI+KE+AMDPARDIILAYMQNG+ L+PDHGFPVR+II
Sbjct: 241 GALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLAPDHGFPVRMII 300

Query: 301 PGFIGGRMVKWVKRIIVTTQESDSYYHYRDNKVLPSHVDEELANAEAWWYKPECIITELN 360
           PGFIGGRMVKW+KRIIVTT+ES++YYH++DN+VLPSHVD ELANAEAWWYKPE II ELN
Sbjct: 301 PGFIGGRMVKWLKRIIVTTKESENYYHFKDNRVLPSHVDAELANAEAWWYKPEYIINELN 360

Query: 361 INSVITTPCHEEILPINSWTTQRPYTLRGYAYSGGGKKVTRVEVTLDGGESWHVCDLDCP 420
           INSVITTPCHEEILPINSWTTQRPYTLRGY+YSGGGKKVTRVEVT+DGGE+  VC LD P
Sbjct: 361 INSVITTPCHEEILPINSWTTQRPYTLRGYSYSGGGKKVTRVEVTMDGGETRQVCTLDHP 420

Query: 421 EKATKYGKYWCWCFWSLEVEVLDLLAAKEIAVRAWDETFNTQPEKLIWNLMGMMNNCWFQ 480
           EK  KYGKYWCWCFWSLEVEVLDLL AKEIAVRAWDET NTQPEKLIWNLMGMMNNCWF 
Sbjct: 421 EKPNKYGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNCWFT 480

Query: 481 VKTNMCKPHKGEIGIVFEHPTVPGNQSGGWMGKERHLEISTESNQTMKKSISSPFINTTS 540
           VKTN+CKPHKGEIGI+FEHPTVPGNQSGGWM +ERHLEISTESNQT+KKSIS+PF+NT S
Sbjct: 481 VKTNVCKPHKGEIGIIFEHPTVPGNQSGGWMDRERHLEISTESNQTLKKSISTPFMNTAS 540

Query: 541 KTYSISDVQKHNSDQSAWIVVHGHVYDCTRFLKDHPGGMDSILINAGTDCTEEFDAIHSD 600
            TYS+S+V+KHNS QSAWI+VHGHVYDCTRFLKDHPGG DSILINAGTDCTEEFDAIHSD
Sbjct: 541 NTYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAIHSD 600

Query: 601 KAKKMLEEYRIGELITTGYISDS---SPNNSIHGDSFSSNPHLAPITE----TRRVALIP 660
           KAKKMLE+YRIGELITTGY SDS   SPNNS HG S  S  HLAPI E    TRR AL P
Sbjct: 601 KAKKMLEDYRIGELITTGYASDSSSNSPNNSTHGASNFS--HLAPIREAPMVTRRAALAP 660

Query: 661 REKIRCKLVSKTSISHDVRRFRIGLP-LDDQILGLPVGKHIFLCANIDGKLCMRAYTPTT 720
            EKI CKL+SKT ISHDVR FR  LP   DQ+LGLPVGKHIF+CA +DGKLCMRAYTP++
Sbjct: 661 NEKIPCKLISKTEISHDVRVFRFELPGGQDQVLGLPVGKHIFICAKVDGKLCMRAYTPSS 720

Query: 721 CINQVGYFDLVVKIYFKNVNPKFPNGGLMSQHLESLPLGSELEIKGPLGHIEYTGNGNFV 780
            ++Q+GYF+LVVK+YFKNV+PKFPNGG+MSQ L+++ +GS +E+KGPLGHIEYTG GNF 
Sbjct: 721 TVDQMGYFELVVKVYFKNVHPKFPNGGIMSQFLDNMEVGSTVEVKGPLGHIEYTGRGNFT 780

Query: 781 VDGRVKKAKKLAMLAGGTGITPIYQVAQAVLKDPEDETEMFLVYANRTENDILLKDELDE 840
           V G+ + AK+LAMLAGGTGITPIYQ+ QA+LKDPEDETEMF+VYANRTE+DILL++ELD 
Sbjct: 781 VHGKPRFAKRLAMLAGGTGITPIYQIVQAILKDPEDETEMFVVYANRTEDDILLREELDT 840

Query: 841 WSKRDKRFKVWYVVNESVREGWEYSVGVVRESIMREHLPEASPETMALACGPPPMIKFAV 894
           W+K+++R KVWYVV ES+REGWEYS+G + E I REHLP A+ +T+AL CGPPPMI+FAV
Sbjct: 841 WAKKNERLKVWYVVQESIREGWEYSIGFITEEITREHLPAAAEDTLALVCGPPPMIQFAV 900

BLAST of Cucsa.274720 vs. TrEMBL
Match: V4TCH9_9ROSI (Nitrate reductase OS=Citrus clementina GN=CICLE_v10000220mg PE=3 SV=1)

HSP 1 Score: 1454.9 bits (3765), Expect = 0.0e+00
Identity = 693/909 (76.24%), Postives = 785/909 (86.36%), Query Frame = 1

Query: 1   MATSINNPQFSHLDSFS----------NLRSDSSLRCCNFPSIPTNHLLINNHDDNEDFS 60
           MA S++N QF+HL+S +          N RSDS +R       P   + +      ED+S
Sbjct: 1   MAASVDNRQFTHLESLNGIVRPYKPGPNHRSDSPIRSSQNGFAPKKQVFLE-----EDWS 60

Query: 61  SDDDNDNDSDFTHLIRKGIQELEPSILDPRDFGTADCWIHRNESLVRLTGKHPFNSEPPL 120
           SDD+N+  +D+  +IRKG  ELEPSILDPRD GTAD WI RN S+VRLTGKHPFN E PL
Sbjct: 61  SDDENE--ADYMEIIRKGNSELEPSILDPRDEGTADNWIERNPSMVRLTGKHPFNCEAPL 120

Query: 121 SRLMHHGFITPVPLHYVRNHGPVPKAKWEDWTVEVCGLVKKPTRFTMNQLVSDFSSREFP 180
           +RLM HGFITPVPLHYVRNHGPVPKA W DWT+EV GLVK+P RFTM+QL+ +F SREFP
Sbjct: 121 TRLMQHGFITPVPLHYVRNHGPVPKASWVDWTIEVTGLVKRPARFTMDQLMHEFPSREFP 180

Query: 181 VTLVCAGNRRREHNMVKKTIGFNWGAGGTSTSVWRGVPLRDVLKRCGIFSRKKGALNVCF 240
           VTLVCAGNRR+E NMVK+TIGFNWGA G STSVWRGVPLR VLKRCGIFSRK GALNVCF
Sbjct: 181 VTLVCAGNRRKEQNMVKQTIGFNWGAAGVSTSVWRGVPLRHVLKRCGIFSRKSGALNVCF 240

Query: 241 EGAECLPGGGGSKYGTSIRKEIAMDPARDIILAYMQNGDVLSPDHGFPVRVIIPGFIGGR 300
           EGAE LPGGGGSKYGTSIRKE+ MDPARDIILAYMQNG++LSPDHGFPVRVIIPG IGGR
Sbjct: 241 EGAEDLPGGGGSKYGTSIRKEVGMDPARDIILAYMQNGELLSPDHGFPVRVIIPGCIGGR 300

Query: 301 MVKWVKRIIVTTQESDSYYHYRDNKVLPSHVDEELANAEAWWYKPECIITELNINSVITT 360
           MVKW+KRIIVTTQESD+YYHY+DN+VLPSHVD ELANAEAWWYKPE II ELNINSVITT
Sbjct: 301 MVKWLKRIIVTTQESDNYYHYKDNRVLPSHVDAELANAEAWWYKPEYIINELNINSVITT 360

Query: 361 PCHEEILPINSWTTQRPYTLRGYAYSGGGKKVTRVEVTLDGGESWHVCDLDCPEKATKYG 420
           PCHEEILPINSWT QRPYTLRGYAYSGGGKKVTRVEVTLDGG++WHVC LD PE+  KYG
Sbjct: 361 PCHEEILPINSWTAQRPYTLRGYAYSGGGKKVTRVEVTLDGGDTWHVCTLDHPEQPNKYG 420

Query: 421 KYWCWCFWSLEVEVLDLLAAKEIAVRAWDETFNTQPEKLIWNLMGMMNNCWFQVKTNMCK 480
           KYWCWCFWSLE+EVLDLL AK+IAVRAWDET NTQPEKLIWN+MGMMNNCWF+VK N+CK
Sbjct: 421 KYWCWCFWSLELEVLDLLGAKDIAVRAWDETLNTQPEKLIWNVMGMMNNCWFRVKANVCK 480

Query: 481 PHKGEIGIVFEHPTVPGNQSGGWMGKERHLEISTESNQTMKKSISSPFINTTSKTYSISD 540
            HKGEIGIVFEHPT PGNQSGGWM KERHL  S+++NQT+KKS+SSPF+NTTS TYS+S+
Sbjct: 481 AHKGEIGIVFEHPTQPGNQSGGWMAKERHLGRSSDANQTLKKSVSSPFMNTTSNTYSLSE 540

Query: 541 VQKHNSDQSAWIVVHGHVYDCTRFLKDHPGGMDSILINAGTDCTEEFDAIHSDKAKKMLE 600
           V KHNS  SAWI+VHGH+YDCTRFLKDHPGG DSILINAGTDCTEEFDAIHSDKAKKMLE
Sbjct: 541 VAKHNSADSAWIIVHGHIYDCTRFLKDHPGGTDSILINAGTDCTEEFDAIHSDKAKKMLE 600

Query: 601 EYRIGELITTGYISD---SSPNNSIHGDSFSSNPHLAPITE---TRRVALIPREKIRCKL 660
           +YRIGELITTGY SD   SSPNNS+HG S     +LAPI E   TR VAL+PREKI CKL
Sbjct: 601 DYRIGELITTGYTSDSSASSPNNSMHGASNYIGTNLAPIKEVAPTRSVALVPREKIPCKL 660

Query: 661 VSKTSISHDVRRFRIGLPLDDQILGLPVGKHIFLCANIDGKLCMRAYTPTTCINQVGYFD 720
           V K  ISHDVR FR  LP +DQ+LGLPVGKHIFLCA ID KLCMRAYTPT+ +++VG+FD
Sbjct: 661 VEKILISHDVRLFRFALPQEDQVLGLPVGKHIFLCATIDDKLCMRAYTPTSSVDEVGHFD 720

Query: 721 LVVKIYFKNVNPKFPNGGLMSQHLESLPLGSELEIKGPLGHIEYTGNGNFVVDGRVKKAK 780
           LVVKIYFK V+PKFPNGGLMSQ+L+SL +GS L++KGPLGHI+YTG GNF+V G+ K AK
Sbjct: 721 LVVKIYFKGVHPKFPNGGLMSQYLDSLSVGSILDVKGPLGHIQYTGRGNFLVHGKPKFAK 780

Query: 781 KLAMLAGGTGITPIYQVAQAVLKDPEDETEMFLVYANRTENDILLKDELDEWSKRDKRFK 840
           KLAMLAGGTGITPIYQ+ QA+LKDPEDETEM++VYANRTE+DILL++ELD+W+K+  R K
Sbjct: 781 KLAMLAGGTGITPIYQIMQAILKDPEDETEMYVVYANRTEDDILLREELDDWAKKHDRVK 840

Query: 841 VWYVVNESVREGWEYSVGVVRESIMREHLPEASPETMALACGPPPMIKFAVKPSLEKMNY 894
           VWYVV +S++EGW+YS G + E+I+REH+PE S +T+ALACGPPPMI+FAV+P+LEKMNY
Sbjct: 841 VWYVVEKSIKEGWQYSTGFITEAILREHVPEGSGDTLALACGPPPMIQFAVQPNLEKMNY 900

BLAST of Cucsa.274720 vs. TAIR10
Match: AT1G77760.1 (AT1G77760.1 nitrate reductase 1)

HSP 1 Score: 1343.6 bits (3476), Expect = 0.0e+00
Identity = 627/860 (72.91%), Postives = 748/860 (86.98%), Query Frame = 1

Query: 44  DDNEDFSSDDD----NDNDSDFTHLIRKGIQELEPSILDPRDFGTADCWIHRNESLVRLT 103
           D  +D SSDD+    N N   +  L++K   +LEPSILDPRD  TAD WI RN S++RLT
Sbjct: 62  DSYDDSSSDDEDESHNRNVPYYKELVKKSNSDLEPSILDPRDESTADSWIQRNSSMLRLT 121

Query: 104 GKHPFNSEPPLSRLMHHGFITPVPLHYVRNHGPVPKAKWEDWTVEVCGLVKKPTRFTMNQ 163
           GKHPFN+E PL RLMHHGFITPVPLHYVRNHG VPKA W DW++E+ GLVK+P +FTM +
Sbjct: 122 GKHPFNAEAPLPRLMHHGFITPVPLHYVRNHGAVPKANWSDWSIEITGLVKRPAKFTMEE 181

Query: 164 LVSDFSSREFPVTLVCAGNRRREHNMVKKTIGFNWGAGGTSTSVWRGVPLRDVLKRCGIF 223
           L+S+F SREFPVTLVCAGNRR+E NMVK+TIGFNWG+ G STS+W+G+PL ++L+RCGI+
Sbjct: 182 LISEFPSREFPVTLVCAGNRRKEQNMVKQTIGFNWGSAGVSTSLWKGIPLSEILRRCGIY 241

Query: 224 SRKKGALNVCFEGAECLPGGGGSKYGTSIRKEIAMDPARDIILAYMQNGDVLSPDHGFPV 283
           SR+ GALNVCFEGAE LPGGGGSKYGTSI+KE+AMDPARDIILAYMQNG++L+PDHGFPV
Sbjct: 242 SRRGGALNVCFEGAEDLPGGGGSKYGTSIKKEMAMDPARDIILAYMQNGELLTPDHGFPV 301

Query: 284 RVIIPGFIGGRMVKWVKRIIVTTQESDSYYHYRDNKVLPSHVDEELANAEAWWYKPECII 343
           RVI+PGFIGGRMVKW+KRIIVT QESDSYYHY+DN+VLPS VD ELAN+EAWWYKPE II
Sbjct: 302 RVIVPGFIGGRMVKWLKRIIVTPQESDSYYHYKDNRVLPSLVDAELANSEAWWYKPEYII 361

Query: 344 TELNINSVITTPCHEEILPINSWTTQRPYTLRGYAYSGGGKKVTRVEVTLDGGESWHVCD 403
            ELNINSVITTP H EILPIN++TTQ+PYTL+GYAYSGGGKKVTRVEVTLDGG++W VC+
Sbjct: 362 NELNINSVITTPGHAEILPINAFTTQKPYTLKGYAYSGGGKKVTRVEVTLDGGDTWSVCE 421

Query: 404 LDCPEKATKYGKYWCWCFWSLEVEVLDLLAAKEIAVRAWDETFNTQPEKLIWNLMGMMNN 463
           LD  EK  KYGK+WCWCFWSL+VEVLDLL+AK++AVRAWDE+FNTQP+KLIWNLMGMMNN
Sbjct: 422 LDHQEKPNKYGKFWCWCFWSLDVEVLDLLSAKDVAVRAWDESFNTQPDKLIWNLMGMMNN 481

Query: 464 CWFQVKTNMCKPHKGEIGIVFEHPTVPGNQSGGWMGKERHLEISTESNQTMKKSISSPFI 523
           CWF+++TN+CKPH+GEIGIVFEHPT PGNQSGGWM KER LEIS+ESN T+KKS+SSPF+
Sbjct: 482 CWFRIRTNVCKPHRGEIGIVFEHPTRPGNQSGGWMAKERQLEISSESNNTLKKSVSSPFM 541

Query: 524 NTTSKTYSISDVQKHNSDQSAWIVVHGHVYDCTRFLKDHPGGMDSILINAGTDCTEEFDA 583
           NT SK YSIS+V+KHN+  SAWI+VHGH+YDCTRFLKDHPGG DSILINAGTDCTEEF+A
Sbjct: 542 NTASKMYSISEVRKHNTADSAWIIVHGHIYDCTRFLKDHPGGTDSILINAGTDCTEEFEA 601

Query: 584 IHSDKAKKMLEEYRIGELITTGYISDSSPNNSIHGDSFSSNPHLAPI---TETRRVALI- 643
           IHSDKAKK+LE+YRIGELITTGY  DSSPN S+HG S +  P LAPI   T  + +AL+ 
Sbjct: 602 IHSDKAKKLLEDYRIGELITTGY--DSSPNVSVHGAS-NFGPLLAPIKELTPQKNIALVN 661

Query: 644 PREKIRCKLVSKTSISHDVRRFRIGLPLDDQILGLPVGKHIFLCANIDGKLCMRAYTPTT 703
           PREKI  +L+ KTSISHDVR+FR  LP +DQ LGLPVGKH+F+CANI+ KLC+RAYTPT+
Sbjct: 662 PREKIPVRLIEKTSISHDVRKFRFALPSEDQQLGLPVGKHVFVCANINDKLCLRAYTPTS 721

Query: 704 CINQVGYFDLVVKIYFKNVNPKFPNGGLMSQHLESLPLGSELEIKGPLGHIEYTGNGNFV 763
            I+ VG+ DLVVK+YFK+V+P+FPNGGLMSQHL+SLP+GS ++IKGPLGHIEY G GNF+
Sbjct: 722 AIDAVGHIDLVVKVYFKDVHPRFPNGGLMSQHLDSLPIGSMIDIKGPLGHIEYKGKGNFL 781

Query: 764 VDGRVKKAKKLAMLAGGTGITPIYQVAQAVLKDPEDETEMFLVYANRTENDILLKDELDE 823
           V G+ K AKKLAMLAGGTGITPIYQ+ Q++L DPEDETEM++VYANRTE+DIL+++EL+ 
Sbjct: 782 VSGKPKFAKKLAMLAGGTGITPIYQIIQSILSDPEDETEMYVVYANRTEDDILVREELEG 841

Query: 824 W-SKRDKRFKVWYVVNESVREGWEYSVGVVRESIMREHLPEA-SPETMALACGPPPMIKF 883
           W SK  +R K+WYVV E  +EGW YS G + E+++REH+PE    E++ALACGPPPMI+F
Sbjct: 842 WASKHKERLKIWYVV-EIAKEGWSYSTGFITEAVLREHIPEGLEGESLALACGPPPMIQF 901

Query: 884 AVKPSLEKMNYDTTNSLLVF 894
           A++P+LEKM Y+    LL+F
Sbjct: 902 ALQPNLEKMGYNVKEDLLIF 917

BLAST of Cucsa.274720 vs. TAIR10
Match: AT1G37130.1 (AT1G37130.1 nitrate reductase 2)

HSP 1 Score: 1331.2 bits (3444), Expect = 0.0e+00
Identity = 642/918 (69.93%), Postives = 759/918 (82.68%), Query Frame = 1

Query: 1   MATSINNPQFSHLDSFSNL-----------RSDSSLRCCNFPSIPTNHLL-INNHDDNED 60
           MA S++N Q++ L+   N            RSDS     N  +  T  L     HDD+ED
Sbjct: 1   MAASVDNRQYARLEPGLNGVVRSYKPPVPGRSDSPKAHQNQTTNQTVFLKPAKVHDDDED 60

Query: 61  FSSDDDNDNDSD----FTHLIRKGIQELEPSILDPRDFGTADCWIHRNESLVRLTGKHPF 120
            SS+D+N+  +     +  +IRK   ELEPS+LDPRD  TAD WI RN S+VRLTGKHPF
Sbjct: 61  VSSEDENETHNSNAVYYKEMIRKSNAELEPSVLDPRDEYTADSWIERNPSMVRLTGKHPF 120

Query: 121 NSEPPLSRLMHHGFITPVPLHYVRNHGPVPKAKWEDWTVEVCGLVKKPTRFTMNQLVSDF 180
           NSE PL+RLMHHGFITPVPLHYVRNHG VPKA+W +WTVEV G VK+P +FTM+QLVS+F
Sbjct: 121 NSEAPLNRLMHHGFITPVPLHYVRNHGHVPKAQWAEWTVEVTGFVKRPMKFTMDQLVSEF 180

Query: 181 SSREFPVTLVCAGNRRREHNMVKKTIGFNWGAGGTSTSVWRGVPLRDVLKRCGIFSRKKG 240
           + REF  TLVCAGNRR+E NMVKK+ GFNWG+ G STSVWRGVPL DVL+RCGIFSRK G
Sbjct: 181 AYREFAATLVCAGNRRKEQNMVKKSKGFNWGSAGVSTSVWRGVPLCDVLRRCGIFSRKGG 240

Query: 241 ALNVCFEGAECLPGGGG---SKYGTSIRKEIAMDPARDIILAYMQNGDVLSPDHGFPVRV 300
           ALNVCFEG+E LPGG G   SKYGTSI+KE AMDP+RDIILAYMQNG+ L+PDHGFPVR+
Sbjct: 241 ALNVCFEGSEDLPGGAGTAGSKYGTSIKKEYAMDPSRDIILAYMQNGEYLTPDHGFPVRI 300

Query: 301 IIPGFIGGRMVKWVKRIIVTTQESDSYYHYRDNKVLPSHVDEELANAEAWWYKPECIITE 360
           IIPGFIGGRMVKW+KRIIVTT+ESD++YH++DN+VLPS VD ELA+ E WWYKPE II E
Sbjct: 301 IIPGFIGGRMVKWLKRIIVTTKESDNFYHFKDNRVLPSLVDAELADEEGWWYKPEYIINE 360

Query: 361 LNINSVITTPCHEEILPINSWTTQRPYTLRGYAYSGGGKKVTRVEVTLDGGESWHVCDLD 420
           LNINSVITTPCHEEILPIN++TTQRPYTL+GYAYSGGGKKVTRVEVT+DGGE+W+VC LD
Sbjct: 361 LNINSVITTPCHEEILPINAFTTQRPYTLKGYAYSGGGKKVTRVEVTVDGGETWNVCALD 420

Query: 421 CPEKATKYGKYWCWCFWSLEVEVLDLLAAKEIAVRAWDETFNTQPEKLIWNLMGMMNNCW 480
             EK  KYGK+WCWCFWSLEVEVLDLL+AKEIAVRAWDET NTQPEK+IWNLMGMMNNCW
Sbjct: 421 HQEKPNKYGKFWCWCFWSLEVEVLDLLSAKEIAVRAWDETLNTQPEKMIWNLMGMMNNCW 480

Query: 481 FQVKTNMCKPHKGEIGIVFEHPTVPGNQSGGWMGKERHLEISTESNQTMKKSISSPFINT 540
           F+VKTN+CKPHKGEIGIVFEHPT+PGN+SGGWM KERHLE S ++  ++KKS+S+PF+NT
Sbjct: 481 FRVKTNVCKPHKGEIGIVFEHPTLPGNESGGWMAKERHLEKSADAPPSLKKSVSTPFMNT 540

Query: 541 TSKTYSISDVQKHNSDQSAWIVVHGHVYDCTRFLKDHPGGMDSILINAGTDCTEEFDAIH 600
           T+K YS+S+V+KHNS  S WI+VHGH+YDCTRFL DHPGG DSILINAGTDCTEEF+AIH
Sbjct: 541 TAKMYSMSEVKKHNSADSCWIIVHGHIYDCTRFLMDHPGGSDSILINAGTDCTEEFEAIH 600

Query: 601 SDKAKKMLEEYRIGELITTGYISD-SSPNNSIHGDS--FSSNPHLAPITETRRVALI-PR 660
           SDKAKKMLE+YRIGELITTGY SD SSPNNS+HG S  FS    +   T  R +AL+ PR
Sbjct: 601 SDKAKKMLEDYRIGELITTGYSSDSSSPNNSVHGSSAVFSLLAPIGEATPVRNLALVNPR 660

Query: 661 EKIRCKLVSKTSISHDVRRFRIGLPLDDQILGLPVGKHIFLCANIDGKLCMRAYTPTTCI 720
            K+  +LV KTSISHDVR+FR  LP++D +LGLPVGKHIFLCA I+ KLC+RAYTP++ +
Sbjct: 661 AKVPVQLVEKTSISHDVRKFRFALPVEDMVLGLPVGKHIFLCATINDKLCLRAYTPSSTV 720

Query: 721 NQVGYFDLVVKIYFKNVNPKFPNGGLMSQHLESLPLGSELEIKGPLGHIEYTGNGNFVVD 780
           + VGYF+LVVKIYF  V+P+FPNGGLMSQ+L+SLP+GS LEIKGPLGH+EY G G+F V 
Sbjct: 721 DVVGYFELVVKIYFGGVHPRFPNGGLMSQYLDSLPIGSTLEIKGPLGHVEYLGKGSFTVH 780

Query: 781 GRVKKAKKLAMLAGGTGITPIYQVAQAVLKDPEDETEMFLVYANRTENDILLKDELDEWS 840
           G+ K A KLAMLAGGTGITP+YQ+ QA+LKDPEDETEM+++YANRTE DILL++ELD W+
Sbjct: 781 GKPKFADKLAMLAGGTGITPVYQIIQAILKDPEDETEMYVIYANRTEEDILLREELDGWA 840

Query: 841 KR-DKRFKVWYVVNESVREGWEYSVGVVRESIMREHLPEA-SPETMALACGPPPMIKFAV 894
           ++   R KVWYVV ES +EGW YS G + E+IMREH+P+      +A+ACGPPPMI+FAV
Sbjct: 841 EQYPDRLKVWYVV-ESAKEGWAYSTGFISEAIMREHIPDGLDGSALAMACGPPPMIQFAV 900

BLAST of Cucsa.274720 vs. TAIR10
Match: AT3G01910.1 (AT3G01910.1 sulfite oxidase)

HSP 1 Score: 204.1 bits (518), Expect = 3.5e-52
Identity = 125/384 (32.55%), Postives = 191/384 (49.74%), Query Frame = 1

Query: 96  VRLTGKHPFNSEPPLSRLMHHGFITPVPLHYVRNHGPVPKAKW-EDWTVEVCGLVKKPTR 155
           +++  K PFN+EPP S L+   ++TPV L Y RNHGP+P     + ++V + GL++ P +
Sbjct: 20  LKVNAKEPFNAEPPRSALVS-SYVTPVDLFYKRNHGPIPIVDHLQSYSVTLTGLIQNPRK 79

Query: 156 FTMNQLVSDFSSREFPVTLVCAGNRRREHNMVKKTIGFNWGAGGTSTSVWRGVPLRDVLK 215
             +  + S         TL CAGNRR   + V+   G  W       +VW G  L DVL+
Sbjct: 80  LFIKDIRS-LPKYNVTATLQCAGNRRTAMSKVRNVRGVGWDVSAIGNAVWGGAKLADVLE 139

Query: 216 RCGI----FSRKKGALNVCFEGAECLPGGGGSKYGTSIRKEIAMDPARDIILAYMQNGDV 275
             GI     S   GA +V F   +      G  Y  SI    A +P  D++LAY  NG+ 
Sbjct: 140 LVGIPKLTASTNLGARHVEFVSVDRCKEENGGPYKASITLSQATNPEADVLLAYEMNGET 199

Query: 276 LSPDHGFPVRVIIPGFIGGRMVKWVKRIIVTTQESDSYYHYRDNKVLPSHVDEELANAEA 335
           L+ DHGFP+RV++PG IG R VKW+  I V  +ES  ++  +D K+ P  V+ +  N  +
Sbjct: 200 LNRDHGFPLRVVVPGVIGARSVKWLDSINVIAEESQGFFMQKDYKMFPPSVNWDNINWSS 259

Query: 336 WWYKPECIITELNINSVITTPCHEEILPINSWTTQRP--YTLRGYAYSGGGKKVTRVEVT 395
              +P+           +  P    I  +      +P   +++GYA SGGG+ + RV+++
Sbjct: 260 --RRPQ-----------MDFPVQSAICSVEDVQMVKPGKVSIKGYAVSGGGRGIERVDIS 319

Query: 396 LDGGESWHVCDLDCPEKATKY------GKYWCWCFWSLEVEVLDLLAAKEIAVRAWDETF 455
           LDGG++W V      E   +Y         W W  +      +D+    E+  +A D   
Sbjct: 320 LDGGKNW-VEASRTQEPGKQYISEHSSSDKWAWVLFE---ATIDVSQTTEVIAKAVDSAA 379

Query: 456 NTQPEKL--IWNLMGMMNNCWFQV 465
           N QPE +  +WNL G++N  W +V
Sbjct: 380 NVQPENVESVWNLRGVLNTSWHRV 384

BLAST of Cucsa.274720 vs. TAIR10
Match: AT5G17770.1 (AT5G17770.1 NADH:cytochrome B5 reductase 1)

HSP 1 Score: 172.2 bits (435), Expect = 1.5e-42
Identity = 102/261 (39.08%), Postives = 155/261 (59.39%), Query Frame = 1

Query: 627 TETRRVALIPREKIRCKLVSKTSISHDVRRFRIGLPLDDQILGLPVGKHIFLCANIDGKL 686
           ++ RRV L P      KLV +  +SH+V +F   LP    +LGLP+G+HI  C   DG+ 
Sbjct: 35  SKKRRVCLDPENFKEFKLVKRHQLSHNVAKFVFELPTSTSVLGLPIGQHIS-CRGKDGQG 94

Query: 687 --CMRAYTPTTCINQVGYFDLVVKIYFKNVNPKFPNGGLMSQHLESLPLGSELEIKGPLG 746
              ++ YTPTT  + VG F+LV+K+Y +         G MS H   + +G  L +KGP G
Sbjct: 95  EDVIKPYTPTTLDSDVGRFELVIKMYPQ---------GRMSHHFREMRVGDHLAVKGPKG 154

Query: 747 HIEYTGNGNFVVDGRVKKAKKLAMLAGGTGITPIYQVAQAVLKDPEDETEMFLVYANRTE 806
             +Y   G F         +   MLAGG+GITP++QVA+A+L++P D+T++ L+YAN T 
Sbjct: 155 RFKYQP-GQF---------RAFGMLAGGSGITPMFQVARAILENPTDKTKVHLIYANVTY 214

Query: 807 NDILLKDELDEWSKR-DKRFKVWYVVNESVREGWEYSVGVVRESIMREHLPEASPETMAL 866
           +DILLK+EL+  +    ++FK++YV+N+   E W+  VG V + +++ H P  + +   L
Sbjct: 215 DDILLKEELEGLTTNYPEQFKIFYVLNQP-PEVWDGGVGFVSKEMIQTHCPAPASDIQIL 273

Query: 867 ACGPPPMIKFAVKPSLEKMNY 885
            CGPPPM K A+  +LE + Y
Sbjct: 275 RCGPPPMNK-AMAANLEALGY 273

BLAST of Cucsa.274720 vs. TAIR10
Match: AT5G20080.1 (AT5G20080.1 FAD/NAD(P)-binding oxidoreductase)

HSP 1 Score: 137.5 bits (345), Expect = 4.0e-32
Identity = 87/248 (35.08%), Postives = 133/248 (53.63%), Query Frame = 1

Query: 631 RVALIPREKIRCKLVSKTSISHDVRRFRIGLPLDDQILGLPVGKHIFLCA----NIDGKL 690
           + AL P + +  KL     +SH+ + FR       + LGL V   +   A    N +GK 
Sbjct: 70  KTALNPDKWLEFKLQDTARVSHNTQLFRFSFDPSAE-LGLHVASCLLTRAPLGYNAEGKT 129

Query: 691 --CMRAYTPTTCINQVGYFDLVVKIYFKNVNPKFPNGGLMSQHLESLPLGSELEIKGPLG 750
              +R YTP +     GYFDL++K+Y        P+G  MSQH  SL  G  LE+KGP+ 
Sbjct: 130 KYVIRPYTPISDPEAKGYFDLLIKVY--------PDGK-MSQHFASLKPGDVLEVKGPVE 189

Query: 751 HIEYTGNGNFVVDGRVKKAKKLAMLAGGTGITPIYQVAQAVLKDPEDETEMFLVYANRTE 810
             +Y+ N            K + M+AGG+GITP+ QV  A++K+PED T++ L+YAN + 
Sbjct: 190 KFKYSPN----------MKKHIGMIAGGSGITPMLQVIDAIVKNPEDNTQISLLYANVSP 249

Query: 811 NDILLKDELDEWSKRDKRFKVWYVVNESVREGWEYSVGVVRESIMREHLPEASPETMALA 870
           +DILLK +LD         K++Y V+   +  W+  VG + + +  + LP  + +T+ L 
Sbjct: 250 DDILLKQKLDVLQANHPNLKIFYTVDNPTK-NWKGGVGYISKDMALKGLPLPTDDTLILV 296

Query: 871 CGPPPMIK 873
           CGPP M++
Sbjct: 310 CGPPGMME 296

BLAST of Cucsa.274720 vs. NCBI nr
Match: gi|778689095|ref|XP_011652901.1| (PREDICTED: uncharacterized protein LOC101216001 isoform X1 [Cucumis sativus])

HSP 1 Score: 1868.6 bits (4839), Expect = 0.0e+00
Identity = 893/893 (100.00%), Postives = 893/893 (100.00%), Query Frame = 1

Query: 1   MATSINNPQFSHLDSFSNLRSDSSLRCCNFPSIPTNHLLINNHDDNEDFSSDDDNDNDSD 60
           MATSINNPQFSHLDSFSNLRSDSSLRCCNFPSIPTNHLLINNHDDNEDFSSDDDNDNDSD
Sbjct: 1   MATSINNPQFSHLDSFSNLRSDSSLRCCNFPSIPTNHLLINNHDDNEDFSSDDDNDNDSD 60

Query: 61  FTHLIRKGIQELEPSILDPRDFGTADCWIHRNESLVRLTGKHPFNSEPPLSRLMHHGFIT 120
           FTHLIRKGIQELEPSILDPRDFGTADCWIHRNESLVRLTGKHPFNSEPPLSRLMHHGFIT
Sbjct: 61  FTHLIRKGIQELEPSILDPRDFGTADCWIHRNESLVRLTGKHPFNSEPPLSRLMHHGFIT 120

Query: 121 PVPLHYVRNHGPVPKAKWEDWTVEVCGLVKKPTRFTMNQLVSDFSSREFPVTLVCAGNRR 180
           PVPLHYVRNHGPVPKAKWEDWTVEVCGLVKKPTRFTMNQLVSDFSSREFPVTLVCAGNRR
Sbjct: 121 PVPLHYVRNHGPVPKAKWEDWTVEVCGLVKKPTRFTMNQLVSDFSSREFPVTLVCAGNRR 180

Query: 181 REHNMVKKTIGFNWGAGGTSTSVWRGVPLRDVLKRCGIFSRKKGALNVCFEGAECLPGGG 240
           REHNMVKKTIGFNWGAGGTSTSVWRGVPLRDVLKRCGIFSRKKGALNVCFEGAECLPGGG
Sbjct: 181 REHNMVKKTIGFNWGAGGTSTSVWRGVPLRDVLKRCGIFSRKKGALNVCFEGAECLPGGG 240

Query: 241 GSKYGTSIRKEIAMDPARDIILAYMQNGDVLSPDHGFPVRVIIPGFIGGRMVKWVKRIIV 300
           GSKYGTSIRKEIAMDPARDIILAYMQNGDVLSPDHGFPVRVIIPGFIGGRMVKWVKRIIV
Sbjct: 241 GSKYGTSIRKEIAMDPARDIILAYMQNGDVLSPDHGFPVRVIIPGFIGGRMVKWVKRIIV 300

Query: 301 TTQESDSYYHYRDNKVLPSHVDEELANAEAWWYKPECIITELNINSVITTPCHEEILPIN 360
           TTQESDSYYHYRDNKVLPSHVDEELANAEAWWYKPECIITELNINSVITTPCHEEILPIN
Sbjct: 301 TTQESDSYYHYRDNKVLPSHVDEELANAEAWWYKPECIITELNINSVITTPCHEEILPIN 360

Query: 361 SWTTQRPYTLRGYAYSGGGKKVTRVEVTLDGGESWHVCDLDCPEKATKYGKYWCWCFWSL 420
           SWTTQRPYTLRGYAYSGGGKKVTRVEVTLDGGESWHVCDLDCPEKATKYGKYWCWCFWSL
Sbjct: 361 SWTTQRPYTLRGYAYSGGGKKVTRVEVTLDGGESWHVCDLDCPEKATKYGKYWCWCFWSL 420

Query: 421 EVEVLDLLAAKEIAVRAWDETFNTQPEKLIWNLMGMMNNCWFQVKTNMCKPHKGEIGIVF 480
           EVEVLDLLAAKEIAVRAWDETFNTQPEKLIWNLMGMMNNCWFQVKTNMCKPHKGEIGIVF
Sbjct: 421 EVEVLDLLAAKEIAVRAWDETFNTQPEKLIWNLMGMMNNCWFQVKTNMCKPHKGEIGIVF 480

Query: 481 EHPTVPGNQSGGWMGKERHLEISTESNQTMKKSISSPFINTTSKTYSISDVQKHNSDQSA 540
           EHPTVPGNQSGGWMGKERHLEISTESNQTMKKSISSPFINTTSKTYSISDVQKHNSDQSA
Sbjct: 481 EHPTVPGNQSGGWMGKERHLEISTESNQTMKKSISSPFINTTSKTYSISDVQKHNSDQSA 540

Query: 541 WIVVHGHVYDCTRFLKDHPGGMDSILINAGTDCTEEFDAIHSDKAKKMLEEYRIGELITT 600
           WIVVHGHVYDCTRFLKDHPGGMDSILINAGTDCTEEFDAIHSDKAKKMLEEYRIGELITT
Sbjct: 541 WIVVHGHVYDCTRFLKDHPGGMDSILINAGTDCTEEFDAIHSDKAKKMLEEYRIGELITT 600

Query: 601 GYISDSSPNNSIHGDSFSSNPHLAPITETRRVALIPREKIRCKLVSKTSISHDVRRFRIG 660
           GYISDSSPNNSIHGDSFSSNPHLAPITETRRVALIPREKIRCKLVSKTSISHDVRRFRIG
Sbjct: 601 GYISDSSPNNSIHGDSFSSNPHLAPITETRRVALIPREKIRCKLVSKTSISHDVRRFRIG 660

Query: 661 LPLDDQILGLPVGKHIFLCANIDGKLCMRAYTPTTCINQVGYFDLVVKIYFKNVNPKFPN 720
           LPLDDQILGLPVGKHIFLCANIDGKLCMRAYTPTTCINQVGYFDLVVKIYFKNVNPKFPN
Sbjct: 661 LPLDDQILGLPVGKHIFLCANIDGKLCMRAYTPTTCINQVGYFDLVVKIYFKNVNPKFPN 720

Query: 721 GGLMSQHLESLPLGSELEIKGPLGHIEYTGNGNFVVDGRVKKAKKLAMLAGGTGITPIYQ 780
           GGLMSQHLESLPLGSELEIKGPLGHIEYTGNGNFVVDGRVKKAKKLAMLAGGTGITPIYQ
Sbjct: 721 GGLMSQHLESLPLGSELEIKGPLGHIEYTGNGNFVVDGRVKKAKKLAMLAGGTGITPIYQ 780

Query: 781 VAQAVLKDPEDETEMFLVYANRTENDILLKDELDEWSKRDKRFKVWYVVNESVREGWEYS 840
           VAQAVLKDPEDETEMFLVYANRTENDILLKDELDEWSKRDKRFKVWYVVNESVREGWEYS
Sbjct: 781 VAQAVLKDPEDETEMFLVYANRTENDILLKDELDEWSKRDKRFKVWYVVNESVREGWEYS 840

Query: 841 VGVVRESIMREHLPEASPETMALACGPPPMIKFAVKPSLEKMNYDTTNSLLVF 894
           VGVVRESIMREHLPEASPETMALACGPPPMIKFAVKPSLEKMNYDTTNSLLVF
Sbjct: 841 VGVVRESIMREHLPEASPETMALACGPPPMIKFAVKPSLEKMNYDTTNSLLVF 893

BLAST of Cucsa.274720 vs. NCBI nr
Match: gi|525507409|ref|NP_001267494.1| (uncharacterized protein LOC101216001 [Cucumis sativus])

HSP 1 Score: 1837.8 bits (4759), Expect = 0.0e+00
Identity = 877/877 (100.00%), Postives = 877/877 (100.00%), Query Frame = 1

Query: 1   MATSINNPQFSHLDSFSNLRSDSSLRCCNFPSIPTNHLLINNHDDNEDFSSDDDNDNDSD 60
           MATSINNPQFSHLDSFSNLRSDSSLRCCNFPSIPTNHLLINNHDDNEDFSSDDDNDNDSD
Sbjct: 1   MATSINNPQFSHLDSFSNLRSDSSLRCCNFPSIPTNHLLINNHDDNEDFSSDDDNDNDSD 60

Query: 61  FTHLIRKGIQELEPSILDPRDFGTADCWIHRNESLVRLTGKHPFNSEPPLSRLMHHGFIT 120
           FTHLIRKGIQELEPSILDPRDFGTADCWIHRNESLVRLTGKHPFNSEPPLSRLMHHGFIT
Sbjct: 61  FTHLIRKGIQELEPSILDPRDFGTADCWIHRNESLVRLTGKHPFNSEPPLSRLMHHGFIT 120

Query: 121 PVPLHYVRNHGPVPKAKWEDWTVEVCGLVKKPTRFTMNQLVSDFSSREFPVTLVCAGNRR 180
           PVPLHYVRNHGPVPKAKWEDWTVEVCGLVKKPTRFTMNQLVSDFSSREFPVTLVCAGNRR
Sbjct: 121 PVPLHYVRNHGPVPKAKWEDWTVEVCGLVKKPTRFTMNQLVSDFSSREFPVTLVCAGNRR 180

Query: 181 REHNMVKKTIGFNWGAGGTSTSVWRGVPLRDVLKRCGIFSRKKGALNVCFEGAECLPGGG 240
           REHNMVKKTIGFNWGAGGTSTSVWRGVPLRDVLKRCGIFSRKKGALNVCFEGAECLPGGG
Sbjct: 181 REHNMVKKTIGFNWGAGGTSTSVWRGVPLRDVLKRCGIFSRKKGALNVCFEGAECLPGGG 240

Query: 241 GSKYGTSIRKEIAMDPARDIILAYMQNGDVLSPDHGFPVRVIIPGFIGGRMVKWVKRIIV 300
           GSKYGTSIRKEIAMDPARDIILAYMQNGDVLSPDHGFPVRVIIPGFIGGRMVKWVKRIIV
Sbjct: 241 GSKYGTSIRKEIAMDPARDIILAYMQNGDVLSPDHGFPVRVIIPGFIGGRMVKWVKRIIV 300

Query: 301 TTQESDSYYHYRDNKVLPSHVDEELANAEAWWYKPECIITELNINSVITTPCHEEILPIN 360
           TTQESDSYYHYRDNKVLPSHVDEELANAEAWWYKPECIITELNINSVITTPCHEEILPIN
Sbjct: 301 TTQESDSYYHYRDNKVLPSHVDEELANAEAWWYKPECIITELNINSVITTPCHEEILPIN 360

Query: 361 SWTTQRPYTLRGYAYSGGGKKVTRVEVTLDGGESWHVCDLDCPEKATKYGKYWCWCFWSL 420
           SWTTQRPYTLRGYAYSGGGKKVTRVEVTLDGGESWHVCDLDCPEKATKYGKYWCWCFWSL
Sbjct: 361 SWTTQRPYTLRGYAYSGGGKKVTRVEVTLDGGESWHVCDLDCPEKATKYGKYWCWCFWSL 420

Query: 421 EVEVLDLLAAKEIAVRAWDETFNTQPEKLIWNLMGMMNNCWFQVKTNMCKPHKGEIGIVF 480
           EVEVLDLLAAKEIAVRAWDETFNTQPEKLIWNLMGMMNNCWFQVKTNMCKPHKGEIGIVF
Sbjct: 421 EVEVLDLLAAKEIAVRAWDETFNTQPEKLIWNLMGMMNNCWFQVKTNMCKPHKGEIGIVF 480

Query: 481 EHPTVPGNQSGGWMGKERHLEISTESNQTMKKSISSPFINTTSKTYSISDVQKHNSDQSA 540
           EHPTVPGNQSGGWMGKERHLEISTESNQTMKKSISSPFINTTSKTYSISDVQKHNSDQSA
Sbjct: 481 EHPTVPGNQSGGWMGKERHLEISTESNQTMKKSISSPFINTTSKTYSISDVQKHNSDQSA 540

Query: 541 WIVVHGHVYDCTRFLKDHPGGMDSILINAGTDCTEEFDAIHSDKAKKMLEEYRIGELITT 600
           WIVVHGHVYDCTRFLKDHPGGMDSILINAGTDCTEEFDAIHSDKAKKMLEEYRIGELITT
Sbjct: 541 WIVVHGHVYDCTRFLKDHPGGMDSILINAGTDCTEEFDAIHSDKAKKMLEEYRIGELITT 600

Query: 601 GYISDSSPNNSIHGDSFSSNPHLAPITETRRVALIPREKIRCKLVSKTSISHDVRRFRIG 660
           GYISDSSPNNSIHGDSFSSNPHLAPITETRRVALIPREKIRCKLVSKTSISHDVRRFRIG
Sbjct: 601 GYISDSSPNNSIHGDSFSSNPHLAPITETRRVALIPREKIRCKLVSKTSISHDVRRFRIG 660

Query: 661 LPLDDQILGLPVGKHIFLCANIDGKLCMRAYTPTTCINQVGYFDLVVKIYFKNVNPKFPN 720
           LPLDDQILGLPVGKHIFLCANIDGKLCMRAYTPTTCINQVGYFDLVVKIYFKNVNPKFPN
Sbjct: 661 LPLDDQILGLPVGKHIFLCANIDGKLCMRAYTPTTCINQVGYFDLVVKIYFKNVNPKFPN 720

Query: 721 GGLMSQHLESLPLGSELEIKGPLGHIEYTGNGNFVVDGRVKKAKKLAMLAGGTGITPIYQ 780
           GGLMSQHLESLPLGSELEIKGPLGHIEYTGNGNFVVDGRVKKAKKLAMLAGGTGITPIYQ
Sbjct: 721 GGLMSQHLESLPLGSELEIKGPLGHIEYTGNGNFVVDGRVKKAKKLAMLAGGTGITPIYQ 780

Query: 781 VAQAVLKDPEDETEMFLVYANRTENDILLKDELDEWSKRDKRFKVWYVVNESVREGWEYS 840
           VAQAVLKDPEDETEMFLVYANRTENDILLKDELDEWSKRDKRFKVWYVVNESVREGWEYS
Sbjct: 781 VAQAVLKDPEDETEMFLVYANRTENDILLKDELDEWSKRDKRFKVWYVVNESVREGWEYS 840

Query: 841 VGVVRESIMREHLPEASPETMALACGPPPMIKFAVKP 878
           VGVVRESIMREHLPEASPETMALACGPPPMIKFAVKP
Sbjct: 841 VGVVRESIMREHLPEASPETMALACGPPPMIKFAVKP 877

BLAST of Cucsa.274720 vs. NCBI nr
Match: gi|659086811|ref|XP_008444121.1| (PREDICTED: nitrate reductase [NADH])

HSP 1 Score: 1761.5 bits (4561), Expect = 0.0e+00
Identity = 841/893 (94.18%), Postives = 865/893 (96.86%), Query Frame = 1

Query: 1   MATSINNPQFSHLDSFSNLRSDSSLRCCNFPSIPTNHLLINNHDDNEDFSSDDDNDNDSD 60
           MATSI+NPQFSHLDSFSNLRSDSSLRCCN PSIP NHL INNHDDNE+FSSDDD  NDSD
Sbjct: 1   MATSIHNPQFSHLDSFSNLRSDSSLRCCNVPSIPNNHLQINNHDDNEEFSSDDD--NDSD 60

Query: 61  FTHLIRKGIQELEPSILDPRDFGTADCWIHRNESLVRLTGKHPFNSEPPLSRLMHHGFIT 120
           F+HLIRKGIQELEPSILDPRDF TADCWIHRNES+VRLTGKHPFNSEP LSRLMHHGFIT
Sbjct: 61  FSHLIRKGIQELEPSILDPRDFRTADCWIHRNESMVRLTGKHPFNSEPSLSRLMHHGFIT 120

Query: 121 PVPLHYVRNHGPVPKAKWEDWTVEVCGLVKKPTRFTMNQLVSDFSSREFPVTLVCAGNRR 180
           PVPLHYVRNHGPVPKAKWEDWTV+VCGLVKKPTRFTM+QLV+DF SREFPVTLVCAGNRR
Sbjct: 121 PVPLHYVRNHGPVPKAKWEDWTVDVCGLVKKPTRFTMDQLVNDFRSREFPVTLVCAGNRR 180

Query: 181 REHNMVKKTIGFNWGAGGTSTSVWRGVPLRDVLKRCGIFSRKKGALNVCFEGAECLPGGG 240
           +E NMVKKTIGF+WGAGGTSTS+WRGV LRDVLKRCGIFS KKGALNVCFEGAECLPGGG
Sbjct: 181 KEQNMVKKTIGFSWGAGGTSTSIWRGVTLRDVLKRCGIFSSKKGALNVCFEGAECLPGGG 240

Query: 241 GSKYGTSIRKEIAMDPARDIILAYMQNGDVLSPDHGFPVRVIIPGFIGGRMVKWVKRIIV 300
           GSKYGTS+RKEIAMDPARDIILAYMQNGDVLSPDHGFPVRVIIPG+IGGRMVKW+KRIIV
Sbjct: 241 GSKYGTSVRKEIAMDPARDIILAYMQNGDVLSPDHGFPVRVIIPGYIGGRMVKWLKRIIV 300

Query: 301 TTQESDSYYHYRDNKVLPSHVDEELANAEAWWYKPECIITELNINSVITTPCHEEILPIN 360
           TTQESDSYYHYRDNKVLPSHVD ELANAEAWWYKPECII+ELNINSVITTPCHEEILPIN
Sbjct: 301 TTQESDSYYHYRDNKVLPSHVDAELANAEAWWYKPECIISELNINSVITTPCHEEILPIN 360

Query: 361 SWTTQRPYTLRGYAYSGGGKKVTRVEVTLDGGESWHVCDLDCPEKATKYGKYWCWCFWSL 420
           S TTQRPYTLRGYAYSGGGKKVTRVEVTLDGGESWH+CDLD PEKATKYGKYWCWCFWSL
Sbjct: 361 SLTTQRPYTLRGYAYSGGGKKVTRVEVTLDGGESWHLCDLDRPEKATKYGKYWCWCFWSL 420

Query: 421 EVEVLDLLAAKEIAVRAWDETFNTQPEKLIWNLMGMMNNCWFQVKTNMCKPHKGEIGIVF 480
           EVEVLDLLAAKEIAVRAWDETFNTQPEKLIWNLMGMMNNCWF+VKTNMC+PHKGEIGIVF
Sbjct: 421 EVEVLDLLAAKEIAVRAWDETFNTQPEKLIWNLMGMMNNCWFRVKTNMCRPHKGEIGIVF 480

Query: 481 EHPTVPGNQSGGWMGKERHLEISTESNQTMKKSISSPFINTTSKTYSISDVQKHNSDQSA 540
           EHPTVPGNQSGGWM KERHLEISTE NQTMKKSISSPFINTTSKTYSISDVQKHNSDQSA
Sbjct: 481 EHPTVPGNQSGGWMAKERHLEISTEPNQTMKKSISSPFINTTSKTYSISDVQKHNSDQSA 540

Query: 541 WIVVHGHVYDCTRFLKDHPGGMDSILINAGTDCTEEFDAIHSDKAKKMLEEYRIGELITT 600
           WIVVHGHVYDCTRFLKDHPGG DSILINAGTDCTEEFDAIHSDKAKKMLEEYRIGELITT
Sbjct: 541 WIVVHGHVYDCTRFLKDHPGGTDSILINAGTDCTEEFDAIHSDKAKKMLEEYRIGELITT 600

Query: 601 GYISDSSPNNSIHGDSFSSNPHLAPITETRRVALIPREKIRCKLVSKTSISHDVRRFRIG 660
           GY SDSSPNNSIHGD  +SNPHL PITETRRVALIPREKIRCKL+SKTSISHDVRRFR  
Sbjct: 601 GYTSDSSPNNSIHGDPSNSNPHLDPITETRRVALIPREKIRCKLISKTSISHDVRRFRFA 660

Query: 661 LPLDDQILGLPVGKHIFLCANIDGKLCMRAYTPTTCINQVGYFDLVVKIYFKNVNPKFPN 720
           LPL+DQ+LGLPVGKHIFLCANIDGKLCMRAYTPTT INQVGYFDLVVKIYFKNVNPKFPN
Sbjct: 661 LPLEDQVLGLPVGKHIFLCANIDGKLCMRAYTPTTGINQVGYFDLVVKIYFKNVNPKFPN 720

Query: 721 GGLMSQHLESLPLGSELEIKGPLGHIEYTGNGNFVVDGRVKKAKKLAMLAGGTGITPIYQ 780
           GGLMSQHLESLPLGSELEIKGPLGHIEYTGNGNFVVDG+VKK KKLAMLAGGTGITPIYQ
Sbjct: 721 GGLMSQHLESLPLGSELEIKGPLGHIEYTGNGNFVVDGKVKKGKKLAMLAGGTGITPIYQ 780

Query: 781 VAQAVLKDPEDETEMFLVYANRTENDILLKDELDEWSKRDKRFKVWYVVNESVREGWEYS 840
           VAQAVLKDPEDETEMFLVYANRTENDILLKDELDEWSKRDKRFKVWYVVNESVREGWEYS
Sbjct: 781 VAQAVLKDPEDETEMFLVYANRTENDILLKDELDEWSKRDKRFKVWYVVNESVREGWEYS 840

Query: 841 VGVVRESIMREHLPEASPETMALACGPPPMIKFAVKPSLEKMNYDTTNSLLVF 894
           VGVV ESIMREHLPEAS ETMALACGPPPMIKFAV+P+LEKMNYDT NSLLVF
Sbjct: 841 VGVVTESIMREHLPEASSETMALACGPPPMIKFAVQPNLEKMNYDTNNSLLVF 891

BLAST of Cucsa.274720 vs. NCBI nr
Match: gi|659120996|ref|XP_008460453.1| (PREDICTED: nitrate reductase [NADH])

HSP 1 Score: 1477.6 bits (3824), Expect = 0.0e+00
Identity = 703/918 (76.58%), Postives = 800/918 (87.15%), Query Frame = 1

Query: 1   MATSINNPQFSHLD-----------SFSNLRSDSSLRCCNFPSIPTNHLLINN------H 60
           MA S++N QF  L            S  N RSDS +R CNFP+   N+ L+ +       
Sbjct: 1   MAASVDNRQFGSLQPPLNGVVRSFKSGPNRRSDSPVRGCNFPNSNINNRLLKSSVKMEQE 60

Query: 61  DDNEDFSSDDDNDNDSDFTHLIRKGIQELEPSILDPRDFGTADCWIHRNESLVRLTGKHP 120
           ++++D  S+D+NDN+ +   LI+KG +ELEPSI+DPRD  TAD WI RN S+VRLTGKHP
Sbjct: 61  EEDDDSCSEDENDNN-ELRELIKKGNRELEPSIVDPRDEATADNWIERNSSMVRLTGKHP 120

Query: 121 FNSEPPLSRLMHHGFITPVPLHYVRNHGPVPKAKWEDWTVEVCGLVKKPTRFTMNQLVSD 180
           FNSEPPL+RLMHHGFITPVPLHYVRNHG VPKA W DWT+EVCGLVK+PT+FTM+QLV++
Sbjct: 121 FNSEPPLNRLMHHGFITPVPLHYVRNHGAVPKANWSDWTIEVCGLVKRPTKFTMDQLVNE 180

Query: 181 FSSREFPVTLVCAGNRRREHNMVKKTIGFNWGAGGTSTSVWRGVPLRDVLKRCGIFSRKK 240
           F  REFP TLVCAGNRR+E NMVKK+IGFNWGA G STSVWRGVPLRDVLKRCGI SRKK
Sbjct: 181 FRFREFPATLVCAGNRRKEQNMVKKSIGFNWGAAGVSTSVWRGVPLRDVLKRCGIMSRKK 240

Query: 241 GALNVCFEGAECLPGGGGSKYGTSIRKEIAMDPARDIILAYMQNGDVLSPDHGFPVRVII 300
           GALNVCFEGAE LPGGGGSKYGTSI+KE+AMDPARDIILAYMQNG+ L PDHGFPVR+II
Sbjct: 241 GALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLVPDHGFPVRMII 300

Query: 301 PGFIGGRMVKWVKRIIVTTQESDSYYHYRDNKVLPSHVDEELANAEAWWYKPECIITELN 360
           PGFIGGRMVKW+KRIIVTT+ES++YYH++DN+VLPSHVD ELANAEAWWYKPE II ELN
Sbjct: 301 PGFIGGRMVKWLKRIIVTTKESENYYHFKDNRVLPSHVDAELANAEAWWYKPEYIINELN 360

Query: 361 INSVITTPCHEEILPINSWTTQRPYTLRGYAYSGGGKKVTRVEVTLDGGESWHVCDLDCP 420
           INSVITTPCHEEILPINSWTTQRPYTLRGY+YSGGGKKVTRVEVT+DGGE+W VC LD P
Sbjct: 361 INSVITTPCHEEILPINSWTTQRPYTLRGYSYSGGGKKVTRVEVTMDGGETWQVCTLDHP 420

Query: 421 EKATKYGKYWCWCFWSLEVEVLDLLAAKEIAVRAWDETFNTQPEKLIWNLMGMMNNCWFQ 480
           EK  KYGKYWCWCFWSLEVEVLDLL AKEIAVRAWDET NTQPEKLIWNLMGMMNNCWF 
Sbjct: 421 EKPNKYGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNCWFT 480

Query: 481 VKTNMCKPHKGEIGIVFEHPTVPGNQSGGWMGKERHLEISTESNQTMKKSISSPFINTTS 540
           VKTN+CKPHKGEIGI+FEHPTVPGNQSGGWM +ERHLEISTESNQT+KKSIS+PF+NT S
Sbjct: 481 VKTNVCKPHKGEIGIIFEHPTVPGNQSGGWMDRERHLEISTESNQTLKKSISTPFMNTAS 540

Query: 541 KTYSISDVQKHNSDQSAWIVVHGHVYDCTRFLKDHPGGMDSILINAGTDCTEEFDAIHSD 600
            TYS+S+V+KHNS QSAWI+VHGHVYDCTRFLKDHPGG DSILINAGTDCTEEFDAIHSD
Sbjct: 541 NTYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAIHSD 600

Query: 601 KAKKMLEEYRIGELITTGYISDS---SPNNSIHGDSFSSNPHLAPITE----TRRVALIP 660
           KAKKMLE+YRIGELITTGY SDS   SPNNS HG S  S  HLAPI E    TRRVAL P
Sbjct: 601 KAKKMLEDYRIGELITTGYASDSSSNSPNNSTHGASNFS--HLAPIREAPVVTRRVALAP 660

Query: 661 REKIRCKLVSKTSISHDVRRFRIGLP-LDDQILGLPVGKHIFLCANIDGKLCMRAYTPTT 720
            EKI CKL+SKT+ISHDVR FR  LP   DQ+LGLPVGKHIF+CA ++GKLCMRAYTP+T
Sbjct: 661 NEKIPCKLISKTAISHDVRVFRFELPGGQDQVLGLPVGKHIFICAKVEGKLCMRAYTPST 720

Query: 721 CINQVGYFDLVVKIYFKNVNPKFPNGGLMSQHLESLPLGSELEIKGPLGHIEYTGNGNFV 780
            ++Q+GYF+LVVK+YF+NV+PKFPNGG+MSQ L+++ +GS +E+KGPLGHIEYTG+GNF 
Sbjct: 721 TVDQMGYFELVVKVYFRNVHPKFPNGGIMSQFLDNMEVGSTVEVKGPLGHIEYTGSGNFT 780

Query: 781 VDGRVKKAKKLAMLAGGTGITPIYQVAQAVLKDPEDETEMFLVYANRTENDILLKDELDE 840
           V G+ + AK+LAMLAGGTGITPIYQV QA+LKDPEDETEMF+VYANRTE+DILL++ELD 
Sbjct: 781 VHGKPRFAKRLAMLAGGTGITPIYQVVQAILKDPEDETEMFVVYANRTEDDILLREELDT 840

Query: 841 WSKRDKRFKVWYVVNESVREGWEYSVGVVRESIMREHLPEASPETMALACGPPPMIKFAV 894
           W+K+++R KVWYVV ES+REGWEYS+G + E IMREHLP A+ +T+AL CGPPPMI+FAV
Sbjct: 841 WAKKNERLKVWYVVQESIREGWEYSIGFITEEIMREHLPAAAEDTLALVCGPPPMIQFAV 900

BLAST of Cucsa.274720 vs. NCBI nr
Match: gi|525507312|ref|NP_001267696.1| (nitrate reductase [NADH])

HSP 1 Score: 1473.8 bits (3814), Expect = 0.0e+00
Identity = 700/918 (76.25%), Postives = 798/918 (86.93%), Query Frame = 1

Query: 1   MATSINNPQFSHLD-----------SFSNLRSDSSLRCCNFPSIPTNHLLINN------H 60
           MA S++N QF  L            S  N RSDS +R CNFP+   N+ L+ +       
Sbjct: 1   MAASVDNRQFGSLQPPLNGVVRSFKSGPNHRSDSPVRGCNFPNSNINNRLLKSSVKMEQE 60

Query: 61  DDNEDFSSDDDNDNDSDFTHLIRKGIQELEPSILDPRDFGTADCWIHRNESLVRLTGKHP 120
           ++++D  S+D+NDN+ +   LI+KG +ELEPS +DPRD GTAD WI RN S+VRLTGKHP
Sbjct: 61  EEDDDSCSEDENDNN-ELRELIKKGNRELEPSTVDPRDEGTADNWIERNSSMVRLTGKHP 120

Query: 121 FNSEPPLSRLMHHGFITPVPLHYVRNHGPVPKAKWEDWTVEVCGLVKKPTRFTMNQLVSD 180
           FNSEPPL+RLMHHGFITPVPLHYVRNHG VPKAKW DWT+EVCGLVK+PT+F+M+QLV++
Sbjct: 121 FNSEPPLNRLMHHGFITPVPLHYVRNHGAVPKAKWSDWTIEVCGLVKRPTKFSMDQLVNE 180

Query: 181 FSSREFPVTLVCAGNRRREHNMVKKTIGFNWGAGGTSTSVWRGVPLRDVLKRCGIFSRKK 240
           F  REFP TLVCAGNRR+E NMVKK+IGFNWGA G STSVWRGVPLR+VLKRCGI SRKK
Sbjct: 181 FRFREFPATLVCAGNRRKEQNMVKKSIGFNWGAAGVSTSVWRGVPLREVLKRCGIMSRKK 240

Query: 241 GALNVCFEGAECLPGGGGSKYGTSIRKEIAMDPARDIILAYMQNGDVLSPDHGFPVRVII 300
           GALNVCFEGAE LPGGGGSKYGTSI+KE+AMDPARDIILAYMQNG+ L+PDHGFPVR+II
Sbjct: 241 GALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLAPDHGFPVRMII 300

Query: 301 PGFIGGRMVKWVKRIIVTTQESDSYYHYRDNKVLPSHVDEELANAEAWWYKPECIITELN 360
           PGFIGGRMVKW+KRIIVTT+ES++YYH++DN+VLPSHVD ELANAEAWWYKPE II ELN
Sbjct: 301 PGFIGGRMVKWLKRIIVTTKESENYYHFKDNRVLPSHVDAELANAEAWWYKPEYIINELN 360

Query: 361 INSVITTPCHEEILPINSWTTQRPYTLRGYAYSGGGKKVTRVEVTLDGGESWHVCDLDCP 420
           INSVITTPCHEEILPINSWTTQRPYTLRGY+YSGGGKKVTRVEVT+DGGE+W VC LD P
Sbjct: 361 INSVITTPCHEEILPINSWTTQRPYTLRGYSYSGGGKKVTRVEVTMDGGETWQVCTLDHP 420

Query: 421 EKATKYGKYWCWCFWSLEVEVLDLLAAKEIAVRAWDETFNTQPEKLIWNLMGMMNNCWFQ 480
           EK  KYGKYWCWCFWSLEVEVLDLL AKEIAVRAWDET NTQPEKLIWNLMGMMNNCWF 
Sbjct: 421 EKPNKYGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNCWFT 480

Query: 481 VKTNMCKPHKGEIGIVFEHPTVPGNQSGGWMGKERHLEISTESNQTMKKSISSPFINTTS 540
           VKTN+CKPHKGEIGI+FEHPTVPGNQSGGWM +ERHLEISTESNQT+KKSIS+PF+NT S
Sbjct: 481 VKTNVCKPHKGEIGIIFEHPTVPGNQSGGWMDRERHLEISTESNQTLKKSISTPFMNTAS 540

Query: 541 KTYSISDVQKHNSDQSAWIVVHGHVYDCTRFLKDHPGGMDSILINAGTDCTEEFDAIHSD 600
            TYS+S+V+KHNS QSAWI+VHGHVYDCTRFLKDHPGG DSILINAGTDCTEEFDAIHSD
Sbjct: 541 NTYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAIHSD 600

Query: 601 KAKKMLEEYRIGELITTGYISDS---SPNNSIHGDSFSSNPHLAPITE----TRRVALIP 660
           KAKKMLE+YRIGELITTGY SDS   SPNNS HG S  S  HLAPI E    TRR AL P
Sbjct: 601 KAKKMLEDYRIGELITTGYASDSSSNSPNNSTHGASNFS--HLAPIREAPMVTRRAALAP 660

Query: 661 REKIRCKLVSKTSISHDVRRFRIGLP-LDDQILGLPVGKHIFLCANIDGKLCMRAYTPTT 720
            EKI CKL+SKT ISHDVR FR  LP   DQ+LGLPVGKHIF+CA +DGKLCMRAYTP++
Sbjct: 661 NEKIPCKLISKTEISHDVRVFRFELPGGQDQVLGLPVGKHIFICAKVDGKLCMRAYTPSS 720

Query: 721 CINQVGYFDLVVKIYFKNVNPKFPNGGLMSQHLESLPLGSELEIKGPLGHIEYTGNGNFV 780
            ++Q+GYF+LVVK+YFKNV+PKFPNGG+MSQ L+++ +GS +E+KGPLGHIEYTG GNF 
Sbjct: 721 TVDQMGYFELVVKVYFKNVHPKFPNGGIMSQFLDNMEVGSTVEVKGPLGHIEYTGRGNFT 780

Query: 781 VDGRVKKAKKLAMLAGGTGITPIYQVAQAVLKDPEDETEMFLVYANRTENDILLKDELDE 840
           V G+ + AK+LAMLAGGTGITPIYQ+ QA+LKDPEDETEMF+VYANRTE+DILL++ELD 
Sbjct: 781 VHGKPRFAKRLAMLAGGTGITPIYQIVQAILKDPEDETEMFVVYANRTEDDILLREELDT 840

Query: 841 WSKRDKRFKVWYVVNESVREGWEYSVGVVRESIMREHLPEASPETMALACGPPPMIKFAV 894
           W+K+++R KVWYVV ES+REGWEYS+G + E I REHLP A+ +T+AL CGPPPMI+FAV
Sbjct: 841 WAKKNERLKVWYVVQESIREGWEYSIGFITEEITREHLPAAAEDTLALVCGPPPMIQFAV 900

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
NIA_CUCMA0.0e+0075.24Nitrate reductase [NADH] OS=Cucurbita maxima PE=2 SV=1[more]
NIA_SOLLC0.0e+0074.48Nitrate reductase [NADH] OS=Solanum lycopersicum GN=NIA PE=3 SV=1[more]
NIA_PETHY0.0e+0074.97Nitrate reductase [NADH] OS=Petunia hybrida GN=NIA PE=2 SV=1[more]
NIA2_TOBAC0.0e+0074.83Nitrate reductase [NADH] 2 OS=Nicotiana tabacum GN=NIA2 PE=2 SV=2[more]
NIA_BETPN0.0e+0074.23Nitrate reductase [NAD(P)H] OS=Betula pendula GN=NIA1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0L1Y7_CUCSA0.0e+00100.00Nitrate reductase OS=Cucumis sativus GN=Csa_4G377160 PE=3 SV=1[more]
E2IZV4_CUCSA0.0e+00100.00Nitrate reductase OS=Cucumis sativus GN=NR1 PE=2 SV=1[more]
E2IZV5_CUCSA0.0e+0076.25Nitrate reductase OS=Cucumis sativus GN=NR2 PE=2 SV=1[more]
E7BXW7_CUCSA0.0e+0076.14Nitrate reductase OS=Cucumis sativus PE=2 SV=1[more]
V4TCH9_9ROSI0.0e+0076.24Nitrate reductase OS=Citrus clementina GN=CICLE_v10000220mg PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G77760.10.0e+0072.91 nitrate reductase 1[more]
AT1G37130.10.0e+0069.93 nitrate reductase 2[more]
AT3G01910.13.5e-5232.55 sulfite oxidase[more]
AT5G17770.11.5e-4239.08 NADH:cytochrome B5 reductase 1[more]
AT5G20080.14.0e-3235.08 FAD/NAD(P)-binding oxidoreductase[more]
Match NameE-valueIdentityDescription
gi|778689095|ref|XP_011652901.1|0.0e+00100.00PREDICTED: uncharacterized protein LOC101216001 isoform X1 [Cucumis sativus][more]
gi|525507409|ref|NP_001267494.1|0.0e+00100.00uncharacterized protein LOC101216001 [Cucumis sativus][more]
gi|659086811|ref|XP_008444121.1|0.0e+0094.18PREDICTED: nitrate reductase [NADH][more]
gi|659120996|ref|XP_008460453.1|0.0e+0076.58PREDICTED: nitrate reductase [NADH][more]
gi|525507312|ref|NP_001267696.1|0.0e+0076.25nitrate reductase [NADH][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000572OxRdtase_Mopterin-bd_dom
IPR001199Cyt_B5-like_heme/steroid-bd
IPR001433OxRdtase_FAD/NAD-bd
IPR001709Flavoprot_Pyr_Nucl_cyt_Rdtase
IPR001834NADH-Cyt_B5_reductase
IPR005066MoCF_OxRdtse_dimer
IPR008333OxRdtase_FAD-bd_dom
IPR008335Mopterin_OxRdtase_euk
IPR012137Nitr_rd_NADH
IPR014756Ig_E-set
IPR017927Fd_Rdtase_FAD-bd
IPR017938Riboflavin_synthase-like_b-brl
IPR018506Cyt_B5_heme-BS
IPR022407OxRdtase_Mopterin_BS
Vocabulary: Biological Process
TermDefinition
GO:0042128nitrate assimilation
GO:0055114oxidation-reduction process
GO:0006809nitric oxide biosynthetic process
Vocabulary: Molecular Function
TermDefinition
GO:0016491oxidoreductase activity
GO:0030151molybdenum ion binding
GO:0050464nitrate reductase (NADPH) activity
GO:0020037heme binding
GO:0043546molybdopterin cofactor binding
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042128 nitrate assimilation
biological_process GO:0006809 nitric oxide biosynthetic process
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0009325 nitrate reductase complex
molecular_function GO:0020037 heme binding
molecular_function GO:0030151 molybdenum ion binding
molecular_function GO:0043546 molybdopterin cofactor binding
molecular_function GO:0009703 nitrate reductase (NADH) activity
molecular_function GO:0050464 nitrate reductase (NADPH) activity
molecular_function GO:0050463 nitrate reductase [NAD(P)H] activity
molecular_function GO:0016491 oxidoreductase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.274720.1Cucsa.274720.1mRNA


Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000572Oxidoreductase, molybdopterin-binding domainGENE3DG3DSA:3.90.420.10coord: 86..334
score: 2.1
IPR000572Oxidoreductase, molybdopterin-binding domainPFAMPF00174Oxidored_molybcoord: 130..309
score: 5.9
IPR000572Oxidoreductase, molybdopterin-binding domainunknownSSF56524Oxidoreductase molybdopterin-binding domaincoord: 78..339
score: 3.53
IPR001199Cytochrome b5-like heme/steroid binding domainPRINTSPR00363CYTOCHROMEB5coord: 558..572
score: 4.1E-18coord: 573..580
score: 4.1E-18coord: 548..558
score: 4.1E-18coord: 585..597
score: 4.1
IPR001199Cytochrome b5-like heme/steroid binding domainGENE3DG3DSA:3.10.120.10coord: 523..597
score: 3.0
IPR001199Cytochrome b5-like heme/steroid binding domainPFAMPF00173Cyt-b5coord: 529..597
score: 4.3
IPR001199Cytochrome b5-like heme/steroid binding domainSMARTSM01117Cyt_b5_2coord: 526..598
score: 5.7
IPR001199Cytochrome b5-like heme/steroid binding domainPROFILEPS50255CYTOCHROME_B5_2coord: 523..598
score: 27
IPR001199Cytochrome b5-like heme/steroid binding domainunknownSSF55856Cytochrome b5-like heme/steroid binding domaincoord: 521..602
score: 1.44
IPR001433Oxidoreductase FAD/NAD(P)-bindingPFAMPF00175NAD_binding_1coord: 768..875
score: 6.8
IPR001709Flavoprotein pyridine nucleotide cytochrome reductasePRINTSPR00371FPNCRcoord: 767..786
score: 2.5E-15coord: 861..869
score: 2.5E-15coord: 793..802
score: 2.5E-15coord: 557..567
score: 2.5E-15coord: 805..816
score: 2.5E-15coord: 689..696
score: 2.5E-15coord: 837..853
score: 2.5
IPR001834NADH:cytochrome b5 reductase (CBR)PRINTSPR00406CYTB5RDTASEcoord: 689..696
score: 4.0E-37coord: 805..816
score: 4.0E-37coord: 668..679
score: 4.0E-37coord: 861..869
score: 4.0E-37coord: 767..786
score: 4.0E-37coord: 734..748
score: 4.0
IPR005066Moybdenum cofactor oxidoreductase, dimerisationGENE3DG3DSA:2.60.40.650coord: 341..468
score: 9.7
IPR005066Moybdenum cofactor oxidoreductase, dimerisationPFAMPF03404Mo-co_dimercoord: 338..467
score: 1.2
IPR008333Oxidoreductase, FAD-binding domainPFAMPF00970FAD_binding_6coord: 642..748
score: 2.3
IPR008335Eukaryotic molybdopterin oxidoreductasePRINTSPR00407EUMOPTERINcoord: 182..194
score: 1.5E-105coord: 384..396
score: 1.5E-105coord: 449..462
score: 1.5E-105coord: 411..421
score: 1.5E-105coord: 272..285
score: 1.5E-105coord: 339..351
score: 1.5E-105coord: 96..110
score: 1.5E-105coord: 171..182
score: 1.5E-105coord: 255..271
score: 1.5E-105coord: 288..301
score: 1.5E-105coord: 118..134
score: 1.5E-105coord: 370..384
score: 1.5E-105coord: 197..212
score: 1.5E-105coord: 436..448
score: 1.5E
IPR012137Nitrate reductase NADH dependentPIRPIRSF000233Nitr_rd_NADHcoord: 14..892
score:
IPR014756Immunoglobulin E-setunknownSSF81296E set domainscoord: 341..466
score: 2.03
IPR017927Ferredoxin reductase-type FAD-binding domainPROFILEPS51384FAD_FRcoord: 637..749
score: 14
IPR017938Riboflavin synthase-like beta-barrelunknownSSF63380Riboflavin synthase domain-likecoord: 629..751
score: 3.94
IPR018506Cytochrome b5, heme-binding sitePROSITEPS00191CYTOCHROME_B5_1coord: 554..561
scor
IPR022407Oxidoreductase, molybdopterin binding sitePROSITEPS00559MOLYBDOPTERIN_EUKcoord: 147..182
scor
NoneNo IPR availableGENE3DG3DSA:2.40.30.10coord: 632..735
score: 4.1
NoneNo IPR availableGENE3DG3DSA:3.40.50.80coord: 736..887
score: 5.0
NoneNo IPR availablePANTHERPTHR19370NADH-CYTOCHROME B5 REDUCTASEcoord: 439..892
score: 8.0E
NoneNo IPR availablePANTHERPTHR19370:SF100SUBFAMILY NOT NAMEDcoord: 439..892
score: 8.0E
NoneNo IPR availableunknownSSF52343Ferredoxin reductase-like, C-terminal NADP-linked domaincoord: 737..887
score: 3.14