Cucsa.258860 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.258860 vs. Swiss-Prot
Match: PHY1_CERPU (Light-sensor Protein kinase OS=Ceratodon purpureus GN=PHY1 PE=3 SV=3)

HSP 1 Score: 166.4 bits (420), Expect = 1.1e-39
Identity = 157/530 (29.62%), Postives = 241/530 (45.47%), Query Frame = 1

Query: 23   SVHINQRQCCLLLDVLSFAYDSVAEEMKQNLRFEEKHTRWKVLD-----QPLRDLNRVFK 82
            S   N +QC  L   L     S +  ++  +   E H     +D     +  + L  + K
Sbjct: 807  SPKFNNKQCQYLAGKLKAVLQSASLFLR--ISHHEHHELGASIDMGRHVEIFKLLLALAK 866

Query: 83   EAEWYIRQCLETKDWWAKVIMLYQNTDCIEFHIHNLLYSITVVVEAIEMAGESSGS---D 142
            E E +I+ C   KD W K  M   N   +  ++ ++ +++ +   A   +  +SGS   D
Sbjct: 867  EIESFIQGCC--KDEWIKAAMTLTN---VSEYVSSMGFNLELCKIAFCKSCAASGSLTLD 926

Query: 143  HDELLKKKLINSIKYRREHKDLKIFKWKFGKQYLVTQDFCNR-IEAVWNEDRWFLLNKIR 202
              E++ K     +K R    D+      F K   V  D   + + +  N+   +LL +++
Sbjct: 927  QIEVICKDEAEVVK-RNASIDVDTL---FAK---VIYDLTEKTLSSDQNDLAIYLLQRLK 986

Query: 203  EKKLMASSKYEQRLTDHLLKNINGSESFNGKLLPSLMLVGSKDYQVRRRLGVGSQ--YKE 262
              K +  S +  R +     N     SF+ K    + + GS        LG GS    ++
Sbjct: 987  RAKPILPS-FSSRPS---WWNFYDDWSFSEKFFQWIQITGS--------LGSGSSATVEK 1046

Query: 263  ILWLGESFAMRHFFGEI-ESLIPEISMLLSLSHPNITRFLCGFTDEEKKECFLIMELMSR 322
             +WLG   A + F+G   E    E+ +L  L HPNIT   C  +   +++C +IMELM  
Sbjct: 1047 AVWLGTPVAKKTFYGRNNEDFKREVEILAELCHPNITSMFC--SPLYRRKCSIIMELMDG 1106

Query: 323  DLSGYVKEICGPRK---RIPFTLPVALDLMLQIARGMEYLHSKKVYHGDLNPCNILVKPR 382
            DL   ++      +     PF++   +D++LQ + GM YLH K + H DL   NILVK  
Sbjct: 1107 DLLALMQRRLDRNEDHDSPPFSILEVVDIILQTSEGMNYLHEKGIIHRDLKSMNILVKSV 1166

Query: 383  AYSTD--GYVHGQVSGFGLPAVKFKNSSNQNESL---PFIWYAPEVLE------EQDQSG 442
              +    GYVH +V+ FGL   K  ++   N++       W APEV+       E + S 
Sbjct: 1167 KVTKSEIGYVHVKVADFGLSKTKDSSTRYSNQTWNRGTNRWMAPEVINLGYESTEGEISF 1226

Query: 443  SAESCKYTEKSDVYSFGMVCFEVLTGKVPFEDSHLQGDKMSRNIRAGERPLFPHSMPKYV 502
              +  KY  KSDVYSFGMVC+EVLTG VPF +     + + R +  G RP  P   P  +
Sbjct: 1227 DGKVPKYPLKSDVYSFGMVCYEVLTGDVPFPEEK-NPNNVKRMVLEGVRPDLPAHCPIEL 1286

Query: 503  TNLTKRCWQTDPNQRPSFTSICRILRYTK-----RFVAMNPDYNSQTDPA 522
              L   CW  DP +RPSF  IC+ L+Y K      F +    Y S  +P+
Sbjct: 1287 KALITDCWNQDPLKRPSFAVICQKLKYLKYLLMTGFSSYQDSYPSTEEPS 1307

BLAST of Cucsa.258860 vs. Swiss-Prot
Match: Y9955_DICDI (Probable serine/threonine-protein kinase DDB_G0267514 OS=Dictyostelium discoideum GN=DDB_G0267514 PE=3 SV=1)

HSP 1 Score: 110.5 bits (275), Expect = 7.1e-23
Identity = 83/281 (29.54%), Postives = 137/281 (48.75%), Query Frame = 1

Query: 232 VGSKDYQVRRRLGVGS---QYKEILWLGESFAMRHF-FGE------IESLIPEISMLLSL 291
           +   + ++  +LG G+    YK  LW G S A++     E      +E    E+++L  L
Sbjct: 657 ISFSELKISSKLGEGTFGVVYKG-LWRGSSVAIKQIKINEDVNNQVLEEFRKELTILSRL 716

Query: 292 SHPNITRFLCGFTDEEKKECFLIMELMSRDLSGYVKEICGPRKRIPFTLPVALDLMLQIA 351
            HPNI   +   T      CF+   L    L   +       K+I   + +   L +QIA
Sbjct: 717 RHPNIVLLMAACTAPPNL-CFITEYLPGGSLYDALHS-----KKIKMNMQLYKKLAIQIA 776

Query: 352 RGMEYLHSKKVYHGDLNPCNILVKPRAYSTDGYVHGQVSGFGLPAVKFKNSSNQNESLPF 411
           +GM YLH   V H D+   N+L+       D +++ ++  FGL  +K K++         
Sbjct: 777 QGMNYLHLSGVIHRDIKSLNLLL-------DEHMNVKICDFGLSKLKSKSTEMTKSIGSP 836

Query: 412 IWYAPEVLEEQDQSGSAESCKYTEKSDVYSFGMVCFEVLTGKVPFEDSHLQGDKMSRNIR 471
           IW +PE+L  +D         YTEK DVY+FG++ +E+ TG++P+  S L   +++  + 
Sbjct: 837 IWMSPELLMGED---------YTEKVDVYAFGIILWELGTGELPY--SGLDSVQLALAVT 896

Query: 472 AGE-RPLFPHSMPKYVTNLTKRCWQTDPNQRPSFTSICRIL 502
               RP  P++ P  +++L + CW  DP +RPSFT I  +L
Sbjct: 897 TKSLRPPIPNAWPYQLSHLIQACWHQDPLKRPSFTEILNLL 912

BLAST of Cucsa.258860 vs. Swiss-Prot
Match: HT1_ARATH (Serine/threonine-protein kinase HT1 OS=Arabidopsis thaliana GN=HT1 PE=1 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 6.6e-21
Identity = 69/228 (30.26%), Postives = 113/228 (49.56%), Query Frame = 1

Query: 274 EISMLLSLSHPNITRFLCGFTDEEKKECFLIMELMSRDLSGYVKEICGPRKRIPFTLPVA 333
           E+++L  L HPNI +F+     ++     +I E MS+   G ++     ++    ++   
Sbjct: 135 EVALLSRLFHPNIVQFIAAC--KKPPVYCIITEYMSQ---GNLRMYLNKKEPYSLSIETV 194

Query: 334 LDLMLQIARGMEYLHSKKVYHGDLNPCNILVKPRAYSTDGYVHGQVSGFGLPAVKFKNSS 393
           L L L I+RGMEYLHS+ V H DL   N+L+          +  +V+ FG   ++ +   
Sbjct: 195 LRLALDISRGMEYLHSQGVIHRDLKSNNLLLNDE-------MRVKVADFGTSCLETQCRE 254

Query: 394 NQNESLPFIWYAPEVLEEQDQSGSAESCKYTEKSDVYSFGMVCFEVLTGKVPFEDSHLQG 453
            +     + W APE+++E+          YT K DVYSFG+V +E+ T  +PF+      
Sbjct: 255 AKGNMGTYRWMAPEMIKEKP---------YTRKVDVYSFGIVLWELTTALLPFQGM-TPV 314

Query: 454 DKMSRNIRAGERPLFPHSMPKYVTNLTKRCWQTDPNQRPSFTSICRIL 502
                     ERP  P S    + +L KRCW  +P++RP F++I  +L
Sbjct: 315 QAAFAVAEKNERPPLPASCQPALAHLIKRCWSENPSKRPDFSNIVAVL 340

BLAST of Cucsa.258860 vs. Swiss-Prot
Match: M3K7_BOVIN (Mitogen-activated protein kinase kinase kinase 7 OS=Bos taurus GN=MAP3K7 PE=2 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 1.1e-20
Identity = 119/487 (24.44%), Postives = 206/487 (42.30%), Query Frame = 1

Query: 216 NGSESFNGKLL--PSLML----VGSKDYQVRRRLGVGS--QYKEILWLGESFAMRHFFGE 275
           + S S  G+++  PS +L    +  K+ +V   +G G+     +  W  +  A++    E
Sbjct: 9   SSSSSSAGEMIEAPSQVLNFEEIDYKEIEVEEVVGRGAFGVVCKAKWRAKDVAIKQIESE 68

Query: 276 IE--SLIPEISMLLSLSHPNITRFLCGFTDEEKKECFLIMELMSRDLSGYVKEICGPRKR 335
            E  + I E+  L  ++HPNI +      +       L+ME       G +  +    + 
Sbjct: 69  SERKAFIVELRQLSRVNHPNIVKLYGACLNP----VCLVMEYAE---GGSLYNVLHGAEP 128

Query: 336 IPF-TLPVALDLMLQIARGMEYLHS---KKVYHGDLNPCNILVKPRAYSTDGYVHGQVSG 395
           +P+ T   A+   LQ ++G+ YLHS   K + H DL P N+L+        G    ++  
Sbjct: 129 LPYYTAAHAMSWCLQCSQGVAYLHSMQPKALIHRDLKPPNLLL------VAGGTVLKICD 188

Query: 396 FGLPAVKFKNSSNQNESLPFIWYAPEVLEEQDQSGSAESCKYTEKSDVYSFGMVCFEVLT 455
           FG       + +N   S    W APEV E  +         Y+EK DV+S+G++ +EV+T
Sbjct: 189 FGTACDIQTHMTNNKGSAA--WMAPEVFEGSN---------YSEKCDVFSWGIILWEVIT 248

Query: 456 GKVPFEDSHLQGDKMSRNIRAGERPLFPHSMPKYVTNLTKRCWQTDPNQRPSFTSICRIL 515
            + PF++      ++   +  G RP    ++PK + +L  RCW  DP+QRPS   I +I+
Sbjct: 249 RRKPFDEIGGPAFRIMWAVHNGTRPPLIKNLPKPIESLMTRCWSKDPSQRPSMEEIVKIM 308

Query: 516 RYTKRFVAMNPDYNSQTDPAMPTVDYCD-------IESGLLRRLPSCGISDAA-SPITDI 575
            +  R+      +    +P      Y D         +G    + S   S+ + + +  +
Sbjct: 309 THLMRY------FPGADEPLQYPCQYSDEGQSNSATSTGSFMDITSTNTSNKSDTNMEQV 368

Query: 576 PFQMFAYRVVEKERAGATFKDTSESGSDASACGDETASSIDDPFPTPVERKLPAP-REGS 635
           P      + +E +      K  SESG    + G    SS++   PT   +++ A   E  
Sbjct: 369 PATNDTIKRLESKLLKNQAKQQSESG--RLSLGASRGSSVESLPPTSEGKRMSADMSEIE 428

Query: 636 RRLSLTKNNSDVRLNKLPG-TPKGRFSRPPQISPRGRSMRMNSESQLMAISPKIRRLSGH 679
            R++ T  N   R   +   T  G  + P Q+S R             + SP +R ++  
Sbjct: 429 ARIAATTGNGQPRRRSIQDLTVTG--TDPGQVSSR-------------SSSPSVRMITTS 448

BLAST of Cucsa.258860 vs. Swiss-Prot
Match: YR818_MIMIV (Putative serine/threonine-protein kinase/receptor R818 OS=Acanthamoeba polyphaga mimivirus GN=MIMI_R818 PE=3 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 1.9e-20
Identity = 81/277 (29.24%), Postives = 129/277 (46.57%), Query Frame = 1

Query: 231  LVGSKDYQVRRRLGVGSQYKEIL--WLGESFAMRHFFGE------IESLIPEISMLLSLS 290
            ++   D Q+ +++GVGS     +  W   + A++ F  +      +     EI+ L  L 
Sbjct: 1388 IINYDDIQIGKQIGVGSYGIVNMGKWKNINVAVKKFVKQKIDEKQMLEFRAEIAFLSQLR 1447

Query: 291  HPNITRFLCGFTDEEKKECFLIMELMSRDLSGYVKEICGPRKRIPFTLPVALDLMLQIAR 350
            HP+I   + G   +    C ++ E M       V +   P  ++       + ++ Q A 
Sbjct: 1448 HPHII-LMIGACLKRPNIC-IVTEFMGNGSLRNVIKTTKPEWKLK------IKMLYQTAL 1507

Query: 351  GMEYLHSKK--VYHGDLNPCNILVKPRAYSTDGYVHGQVSGFGLPAVKFKNSSNQNESLP 410
            G+ YLH+    + H D+ P NILV       D  ++ +++ FG   +K +NS       P
Sbjct: 1508 GIGYLHNSDPIIIHRDIKPSNILV-------DDSMNVKIADFGFARIKEENSVMTRCGTP 1567

Query: 411  FIWYAPEVLEEQDQSGSAESCKYTEKSDVYSFGMVCFEVLTGKVPFEDSHLQGDKMSRNI 470
              W APE++  +         KYTEK DV+SFG+V +EVLT K PF   +    K+S +I
Sbjct: 1568 -CWTAPEIIRGE---------KYTEKVDVFSFGIVMWEVLTCKEPFSGCNFM--KVSMDI 1627

Query: 471  RAGERPLFPHSMPKYVTNLTKRCWQTDPNQRPSFTSI 498
              G RP  P   P   T L K+CW   P++RPS   +
Sbjct: 1628 LEGARPQIPSDCPIDFTKLMKQCWHAKPDKRPSMEDV 1637

HSP 2 Score: 78.6 bits (192), Expect = 3.0e-13
Identity = 68/231 (29.44%), Postives = 105/231 (45.45%), Query Frame = 1

Query: 274  EISMLLSLSHPNITRFLCGFTDEEKKECFLIMELMSRDLSGYVKEICGPRKRIPFTLPVA 333
            EI  + SL HPN+  F+   T        ++ME MS    G + ++ G          + 
Sbjct: 838  EIHRMTSLRHPNVVLFMAAST--RPPNMCIVMEFMSL---GSLYDLLGNELVTEIPPVLR 897

Query: 334  LDLMLQIARGMEYLHSKKVYHGDLNPCNILVKPRAYSTDGYVHGQVSGFGLPAVKFKN-- 393
            + +  Q A+GM +LHS  + H DL   N+L+       D   + +VS FGL  +K  N  
Sbjct: 898  IRIAYQAAKGMHFLHSSDIVHRDLKSLNLLL-------DSKWNVKVSDFGLTKIKDNNKG 957

Query: 394  -SSNQNESLPFI-WYAPEVLEEQDQSGSAESCKYTEKSDVYSFGMVCFEVLTGKVPF--- 453
             SS + +S+  I W APEVL E+      +   Y   +DVYSFG++ +E++T   P+   
Sbjct: 958  KSSTKEDSVCSIQWTAPEVLSEK------QDIDYI-LADVYSFGIIMWELMTRLRPYIGL 1017

Query: 454  EDSHLQGDKMSRNIRAGERPLFPHSMPKYVTNLTKRCWQTDPNQRPSFTSI 498
              + +    +  N+R   +    + M      L   CW  D   RPSF  I
Sbjct: 1018 SPAAIAVAVIRDNLRPEIQEEDINLMSSDYVELVNICWHKDTMIRPSFLEI 1049

BLAST of Cucsa.258860 vs. TrEMBL
Match: A0A0A0L614_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G120430 PE=4 SV=1)

HSP 1 Score: 1382.5 bits (3577), Expect = 0.0e+00
Identity = 678/678 (100.00%), Postives = 678/678 (100.00%), Query Frame = 1

Query: 1   MEQFRQLGEALGSVKALMLFKDSVHINQRQCCLLLDVLSFAYDSVAEEMKQNLRFEEKHT 60
           MEQFRQLGEALGSVKALMLFKDSVHINQRQCCLLLDVLSFAYDSVAEEMKQNLRFEEKHT
Sbjct: 1   MEQFRQLGEALGSVKALMLFKDSVHINQRQCCLLLDVLSFAYDSVAEEMKQNLRFEEKHT 60

Query: 61  RWKVLDQPLRDLNRVFKEAEWYIRQCLETKDWWAKVIMLYQNTDCIEFHIHNLLYSITVV 120
           RWKVLDQPLRDLNRVFKEAEWYIRQCLETKDWWAKVIMLYQNTDCIEFHIHNLLYSITVV
Sbjct: 61  RWKVLDQPLRDLNRVFKEAEWYIRQCLETKDWWAKVIMLYQNTDCIEFHIHNLLYSITVV 120

Query: 121 VEAIEMAGESSGSDHDELLKKKLINSIKYRREHKDLKIFKWKFGKQYLVTQDFCNRIEAV 180
           VEAIEMAGESSGSDHDELLKKKLINSIKYRREHKDLKIFKWKFGKQYLVTQDFCNRIEAV
Sbjct: 121 VEAIEMAGESSGSDHDELLKKKLINSIKYRREHKDLKIFKWKFGKQYLVTQDFCNRIEAV 180

Query: 181 WNEDRWFLLNKIREKKLMASSKYEQRLTDHLLKNINGSESFNGKLLPSLMLVGSKDYQVR 240
           WNEDRWFLLNKIREKKLMASSKYEQRLTDHLLKNINGSESFNGKLLPSLMLVGSKDYQVR
Sbjct: 181 WNEDRWFLLNKIREKKLMASSKYEQRLTDHLLKNINGSESFNGKLLPSLMLVGSKDYQVR 240

Query: 241 RRLGVGSQYKEILWLGESFAMRHFFGEIESLIPEISMLLSLSHPNITRFLCGFTDEEKKE 300
           RRLGVGSQYKEILWLGESFAMRHFFGEIESLIPEISMLLSLSHPNITRFLCGFTDEEKKE
Sbjct: 241 RRLGVGSQYKEILWLGESFAMRHFFGEIESLIPEISMLLSLSHPNITRFLCGFTDEEKKE 300

Query: 301 CFLIMELMSRDLSGYVKEICGPRKRIPFTLPVALDLMLQIARGMEYLHSKKVYHGDLNPC 360
           CFLIMELMSRDLSGYVKEICGPRKRIPFTLPVALDLMLQIARGMEYLHSKKVYHGDLNPC
Sbjct: 301 CFLIMELMSRDLSGYVKEICGPRKRIPFTLPVALDLMLQIARGMEYLHSKKVYHGDLNPC 360

Query: 361 NILVKPRAYSTDGYVHGQVSGFGLPAVKFKNSSNQNESLPFIWYAPEVLEEQDQSGSAES 420
           NILVKPRAYSTDGYVHGQVSGFGLPAVKFKNSSNQNESLPFIWYAPEVLEEQDQSGSAES
Sbjct: 361 NILVKPRAYSTDGYVHGQVSGFGLPAVKFKNSSNQNESLPFIWYAPEVLEEQDQSGSAES 420

Query: 421 CKYTEKSDVYSFGMVCFEVLTGKVPFEDSHLQGDKMSRNIRAGERPLFPHSMPKYVTNLT 480
           CKYTEKSDVYSFGMVCFEVLTGKVPFEDSHLQGDKMSRNIRAGERPLFPHSMPKYVTNLT
Sbjct: 421 CKYTEKSDVYSFGMVCFEVLTGKVPFEDSHLQGDKMSRNIRAGERPLFPHSMPKYVTNLT 480

Query: 481 KRCWQTDPNQRPSFTSICRILRYTKRFVAMNPDYNSQTDPAMPTVDYCDIESGLLRRLPS 540
           KRCWQTDPNQRPSFTSICRILRYTKRFVAMNPDYNSQTDPAMPTVDYCDIESGLLRRLPS
Sbjct: 481 KRCWQTDPNQRPSFTSICRILRYTKRFVAMNPDYNSQTDPAMPTVDYCDIESGLLRRLPS 540

Query: 541 CGISDAASPITDIPFQMFAYRVVEKERAGATFKDTSESGSDASACGDETASSIDDPFPTP 600
           CGISDAASPITDIPFQMFAYRVVEKERAGATFKDTSESGSDASACGDETASSIDDPFPTP
Sbjct: 541 CGISDAASPITDIPFQMFAYRVVEKERAGATFKDTSESGSDASACGDETASSIDDPFPTP 600

Query: 601 VERKLPAPREGSRRLSLTKNNSDVRLNKLPGTPKGRFSRPPQISPRGRSMRMNSESQLMA 660
           VERKLPAPREGSRRLSLTKNNSDVRLNKLPGTPKGRFSRPPQISPRGRSMRMNSESQLMA
Sbjct: 601 VERKLPAPREGSRRLSLTKNNSDVRLNKLPGTPKGRFSRPPQISPRGRSMRMNSESQLMA 660

Query: 661 ISPKIRRLSGHASDSELP 679
           ISPKIRRLSGHASDSELP
Sbjct: 661 ISPKIRRLSGHASDSELP 678

BLAST of Cucsa.258860 vs. TrEMBL
Match: M5X3P7_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa021542mg PE=4 SV=1)

HSP 1 Score: 974.9 bits (2519), Expect = 4.9e-281
Identity = 485/692 (70.09%), Postives = 573/692 (82.80%), Query Frame = 1

Query: 1   MEQFRQLGEALGSVKALMLFKDSVHINQRQCCLLLDVLSFAYDSVAEEMKQNLRFEEKHT 60
           MEQFR++GE LGS+KALM+F+D++ INQRQC LLLD+ S AY+S+AEEMK NLRFEEKHT
Sbjct: 1   MEQFRKIGEDLGSLKALMVFQDNIQINQRQCFLLLDIFSSAYESIAEEMKYNLRFEEKHT 60

Query: 61  RWKVLDQPLRDLNRVFKEAEWYIRQCLETKDWWAKVIMLYQNTDCIEFHIHNLLYSITVV 120
           +W+VL+QPLR+L+R+FKE E YIR CLETKDWWAK I LYQN+DCIEFHIHNLL  + +V
Sbjct: 61  KWRVLEQPLRELHRIFKEGEAYIRHCLETKDWWAKAITLYQNSDCIEFHIHNLLSCMPIV 120

Query: 121 VEAIEMAGESSGSDHDELLKKKLINSIKYRREHKDLKIFKWKFGKQYLVTQDFCNRIEAV 180
           +EAI++AGE SG   DE+ +KK + + KY+ +++D K+FKW+FGKQYL+TQDFCNR +  
Sbjct: 121 IEAIDIAGEISGWGQDEIQRKKTVFADKYKDDYRDWKLFKWRFGKQYLITQDFCNRFDTT 180

Query: 181 WNEDRWFLLNKIREKKLMASSKYEQRLTDHLLKNINGSES--FNGKLLPSLMLVGSKDYQ 240
           W EDRW LL+KIREKKL  S+KY +RL D L K+++GSES   NGKLLPS +LVGSKDYQ
Sbjct: 181 WKEDRWTLLHKIREKKLSGSTKYGKRLIDLLFKSLDGSESQPLNGKLLPSSILVGSKDYQ 240

Query: 241 VRRRLGVGSQYKEILWLGESFAMRHFFGEIESLIPEISMLLSLSHPNITRFLCGFTDEEK 300
           VRRRLG GSQYKEILWLGESFA RHFFG+IE L+PEIS LLSLSHPNI  FLCGFTDEEK
Sbjct: 241 VRRRLGGGSQYKEILWLGESFASRHFFGDIEPLLPEISSLLSLSHPNIVHFLCGFTDEEK 300

Query: 301 KECFLIMELMSRDLSGYVKEICGPRKRIPFTLPVALDLMLQIARGMEYLHSKKVYHGDLN 360
           KECFLIMELMSRDL  ++KEICGPRKR+PF+LPVA+DLMLQIARGMEYLHSKK+YHG+LN
Sbjct: 301 KECFLIMELMSRDLCSHIKEICGPRKRLPFSLPVAVDLMLQIARGMEYLHSKKIYHGELN 360

Query: 361 PCNILVKPRAYSTDGYVHGQVSGFGLPAVKF---KNSSNQNESLPFIWYAPEVLEEQDQS 420
           PCNILVK R  STDGY+  +VSGFGL + K    KN SNQN SLPFIWYAPEVLEE +Q+
Sbjct: 361 PCNILVKARGISTDGYLQAKVSGFGLTSAKSPSQKNLSNQNGSLPFIWYAPEVLEEHEQT 420

Query: 421 GSAESCKYTEKSDVYSFGMVCFEVLTGKVPFEDSHLQGDKMSRNIRAGERPLFPHSMPKY 480
            S+E  KYTEKSDVYSFGMVCFE+LTGKVPFEDSHLQG+KMSRNIRAGERPLFP    +Y
Sbjct: 421 KSSEK-KYTEKSDVYSFGMVCFELLTGKVPFEDSHLQGEKMSRNIRAGERPLFPFYSLRY 480

Query: 481 VTNLTKRCWQTDPNQRPSFTSICRILRYTKRFVAMNPDYNSQTDPAMPTVDYCDIESGLL 540
           VTNLTK+CW +DPN RPSF+SICRILRY KRF+AMNPDYNSQ DP +P VDYC+IESG+L
Sbjct: 481 VTNLTKKCWHSDPNLRPSFSSICRILRYIKRFLAMNPDYNSQLDPPVPMVDYCEIESGIL 540

Query: 541 RRLPSCGISDAASPITDIPFQMFAYRVVEKERAGATFKDTSESGSD-ASACGDETASSID 600
           R++PS   S    P++ IPFQMF YR+ E+ER   T KDTSESGSD AS CGDE  ++ D
Sbjct: 541 RKIPSWK-SCEQPPVSQIPFQMFVYRIAERER--TTLKDTSESGSDGASICGDEMVTTPD 600

Query: 601 DPFPTPVERK-LPAPREGSRRLSLTKNNSDVRLNKLP-----GTPKGRFSRPPQISPRGR 660
           +PFP   ERK L +P    ++L   K +SDV+ N+LP     GTP+GR  RPPQ+SP GR
Sbjct: 601 EPFPPAPERKCLTSPDSMKKKLPFLKKSSDVKANRLPGFGYTGTPRGRSVRPPQMSPCGR 660

Query: 661 --SMRMNSESQLMAISPKIRRL-SGHASDSEL 678
             S+RM+SESQLMA++P+IRR  SGHASDSEL
Sbjct: 661 SISLRMSSESQLMAMTPRIRRTSSGHASDSEL 688

BLAST of Cucsa.258860 vs. TrEMBL
Match: V4U287_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10023409mg PE=4 SV=1)

HSP 1 Score: 953.7 bits (2464), Expect = 1.2e-274
Identity = 464/683 (67.94%), Postives = 560/683 (81.99%), Query Frame = 1

Query: 1   MEQFRQLGEALGSVKALMLFKDSVHINQRQCCLLLDVLSFAYDSVAEEMKQNLRFEEKHT 60
           MEQF ++GE +GS+KALM+F+DS+ INQRQCCLLLD+ SFAYDS+AEEM+QNLRFEE+  
Sbjct: 1   MEQFGRIGEVMGSMKALMVFRDSIQINQRQCCLLLDIFSFAYDSIAEEMRQNLRFEERRA 60

Query: 61  RWKVLDQPLRDLNRVFKEAEWYIRQCLETKDWWAKVIMLYQNTDCIEFHIHNLLYSITVV 120
           +WK+L+QPL++L R+FKE E YI+QCLE +DWWAK I LYQNTDC+EF+IHNLL  I ++
Sbjct: 61  KWKILEQPLKELFRIFKEGENYIKQCLEIRDWWAKAITLYQNTDCVEFYIHNLLSCIPIL 120

Query: 121 VEAIEMAGESSGSDHDELLKKKLINSIKYRREHKDLKIFKWKFGKQYLVTQDFCNRIEAV 180
           +EAIE A E SG D D++ KKKL++S KY++E KD K+F+W++GKQYL+T DFC RI+ V
Sbjct: 121 IEAIETAAEFSGWDPDQMHKKKLVHSGKYKKEWKDHKLFQWRYGKQYLITPDFCYRIDTV 180

Query: 181 WNEDRWFLLNKIREKKLMASSKYEQRLTDHLLKNINGSESFNGKLLPSLMLVGSKDYQVR 240
           W EDRW L NKI++KK+  S+K EQ L D L KN++GS S +GKLLPS +L+ S+DYQVR
Sbjct: 181 WKEDRWILFNKIQQKKISGSTKQEQGLIDVLFKNLDGSGSLSGKLLPSRILIKSEDYQVR 240

Query: 241 RRLGVGSQYKEILWLGESFAMRHFFGEIESLIPEISMLLSLSHPNITRFLCGFTDEEKKE 300
           RRLG GSQYKEILWLGESFA+RHFFG+IE L+PEIS LLSLSHPNI  FLCGFTDEEKKE
Sbjct: 241 RRLGSGSQYKEILWLGESFALRHFFGDIEPLVPEISSLLSLSHPNIMHFLCGFTDEEKKE 300

Query: 301 CFLIMELMSRDLSGYVKEICGPRKRIPFTLPVALDLMLQIARGMEYLHSKKVYHGDLNPC 360
           CFLIMELMSRDL  Y+KEIC PRKRIPF+LPVA+DLMLQIARGMEYLHSKK+YHG+LNP 
Sbjct: 301 CFLIMELMSRDLCSYIKEICCPRKRIPFSLPVAVDLMLQIARGMEYLHSKKIYHGNLNPS 360

Query: 361 NILVKPRAYSTDGYVHGQVSGFGLPAVKF---KNSSNQNESLPFIWYAPEVLEEQDQSGS 420
           NIL+KPR  ST+GY+H ++SGFGL +VK    K+ S    + PFIW+APEVLEE +Q+ S
Sbjct: 361 NILLKPRGASTEGYLHAKISGFGLSSVKNFGPKSPSQSGTTHPFIWHAPEVLEENEQTES 420

Query: 421 AESCKYTEKSDVYSFGMVCFEVLTGKVPFEDSHLQGDKMSRNIRAGERPLFPHSMPKYVT 480
           A + KY+EKSDVYSFGM+CFE+LTGKVPFED+HLQGDKMSRNIRAGERPLFP   PKYVT
Sbjct: 421 ASNSKYSEKSDVYSFGMICFEILTGKVPFEDAHLQGDKMSRNIRAGERPLFPFHSPKYVT 480

Query: 481 NLTKRCWQTDPNQRPSFTSICRILRYTKRFVAMNPDYNSQTDPAMPTVDYCDIESGLLRR 540
           NLTKRCW  DPNQRPSF+SICRILRY KRF+ MNP YNSQ DP MP VDY DIES LLR+
Sbjct: 481 NLTKRCWHADPNQRPSFSSICRILRYIKRFIMMNPHYNSQPDPPMPLVDYSDIESRLLRK 540

Query: 541 LPSCGISDAASPITDIPFQMFAYRVVEKERAGATFKDTSESGSD-ASACGDETASSIDDP 600
            PS    +   PI++IPFQMF YRVVEKE+  ++ KDTS+SGSD AS  GDE  ++ +DP
Sbjct: 541 FPSWETHNVL-PISEIPFQMFVYRVVEKEKISSSPKDTSDSGSDKASVSGDENMTTPEDP 600

Query: 601 FPTPVERK-LPAPREGSRRLSLTKNNSDVRLNKLPGTPKGRFSRPPQISPRGRSMRMNSE 660
            P   ERK LP+P   +++LS  K  S+ ++ K  GTPKGR  RPPQ+ P GRS+RM+SE
Sbjct: 601 SPPVTERKFLPSPEAVNKKLSGVKKLSESKVIKQTGTPKGRAVRPPQLIPCGRSLRMSSE 660

Query: 661 SQLMAISPKIRRL-SGHASDSEL 678
            QLMA+SP+I+R  SGH SDSEL
Sbjct: 661 GQLMAMSPRIQRTSSGHVSDSEL 682

BLAST of Cucsa.258860 vs. TrEMBL
Match: A0A067H3G8_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g038696mg PE=4 SV=1)

HSP 1 Score: 953.7 bits (2464), Expect = 1.2e-274
Identity = 464/683 (67.94%), Postives = 560/683 (81.99%), Query Frame = 1

Query: 1   MEQFRQLGEALGSVKALMLFKDSVHINQRQCCLLLDVLSFAYDSVAEEMKQNLRFEEKHT 60
           MEQF ++GE +GS+KALM+F+DS+ INQRQCCLLLD+ SFAYDS+AEEM+QNLRFEE+  
Sbjct: 1   MEQFGRIGEVMGSMKALMVFRDSIQINQRQCCLLLDIFSFAYDSIAEEMRQNLRFEERRA 60

Query: 61  RWKVLDQPLRDLNRVFKEAEWYIRQCLETKDWWAKVIMLYQNTDCIEFHIHNLLYSITVV 120
           +WK+L+QPL++L R+FKE E YI+QCLE +DWWAK I LYQNTDC+EF+IHNLL  I ++
Sbjct: 61  KWKILEQPLKELFRIFKEGENYIKQCLEIRDWWAKAITLYQNTDCVEFYIHNLLSCIPIL 120

Query: 121 VEAIEMAGESSGSDHDELLKKKLINSIKYRREHKDLKIFKWKFGKQYLVTQDFCNRIEAV 180
           +EAIE A E SG D D++ KKKL++S KY++E KD K+F+W++GKQYL+T DFC RI+ V
Sbjct: 121 IEAIETAAEFSGWDPDQMHKKKLVHSGKYKKEWKDHKLFQWRYGKQYLITPDFCYRIDTV 180

Query: 181 WNEDRWFLLNKIREKKLMASSKYEQRLTDHLLKNINGSESFNGKLLPSLMLVGSKDYQVR 240
           W EDRW L NKI++KK+  S+K EQ L D L KN++GS S +GKLLPS +L+ S+DYQVR
Sbjct: 181 WKEDRWILFNKIQQKKISGSTKQEQGLIDVLFKNLDGSGSLSGKLLPSRILIKSEDYQVR 240

Query: 241 RRLGVGSQYKEILWLGESFAMRHFFGEIESLIPEISMLLSLSHPNITRFLCGFTDEEKKE 300
           RRLG GSQYKEILWLGESFA+RHFFG+IE L+PEIS LLSLSHPNI  FLCGFTDEEKKE
Sbjct: 241 RRLGSGSQYKEILWLGESFALRHFFGDIEPLVPEISSLLSLSHPNIMHFLCGFTDEEKKE 300

Query: 301 CFLIMELMSRDLSGYVKEICGPRKRIPFTLPVALDLMLQIARGMEYLHSKKVYHGDLNPC 360
           CFLIMELMSRDL  Y+KEIC PRKRIPF+LPVA+DLMLQIARGMEYLHSKK+YHG+LNP 
Sbjct: 301 CFLIMELMSRDLCSYIKEICCPRKRIPFSLPVAVDLMLQIARGMEYLHSKKIYHGNLNPS 360

Query: 361 NILVKPRAYSTDGYVHGQVSGFGLPAVKF---KNSSNQNESLPFIWYAPEVLEEQDQSGS 420
           NIL+KPR  ST+GY+H ++SGFGL +VK    K+ S    + PFIW+APEVLEE +Q+ S
Sbjct: 361 NILLKPRGASTEGYLHAKISGFGLSSVKNFGPKSPSQSGTTHPFIWHAPEVLEENEQTES 420

Query: 421 AESCKYTEKSDVYSFGMVCFEVLTGKVPFEDSHLQGDKMSRNIRAGERPLFPHSMPKYVT 480
           A + KY+EKSDVYSFGM+CFE+LTGKVPFED+HLQGDKMSRNIRAGERPLFP   PKYVT
Sbjct: 421 ASNSKYSEKSDVYSFGMICFEILTGKVPFEDAHLQGDKMSRNIRAGERPLFPFHSPKYVT 480

Query: 481 NLTKRCWQTDPNQRPSFTSICRILRYTKRFVAMNPDYNSQTDPAMPTVDYCDIESGLLRR 540
           NLTKRCW  DPNQRPSF+SICRILRY KRF+ MNP YNSQ DP MP VDY DIES LLR+
Sbjct: 481 NLTKRCWHADPNQRPSFSSICRILRYIKRFIMMNPHYNSQPDPPMPLVDYSDIESRLLRK 540

Query: 541 LPSCGISDAASPITDIPFQMFAYRVVEKERAGATFKDTSESGSD-ASACGDETASSIDDP 600
            PS    +   PI++IPFQMF YRVVEKE+  ++ KDTS+SGSD AS  GDE  ++ +DP
Sbjct: 541 FPSWETHNVL-PISEIPFQMFVYRVVEKEKISSSPKDTSDSGSDKASVSGDENMTTPEDP 600

Query: 601 FPTPVERK-LPAPREGSRRLSLTKNNSDVRLNKLPGTPKGRFSRPPQISPRGRSMRMNSE 660
            P   ERK LP+P   +++LS  K  S+ ++ K  GTPKGR  RPPQ+ P GRS+RM+SE
Sbjct: 601 SPPVTERKFLPSPEAVNKKLSGVKKLSESKVIKQTGTPKGRAVRPPQLIPCGRSLRMSSE 660

Query: 661 SQLMAISPKIRRL-SGHASDSEL 678
            QLMA+SP+I+R  SGH SDSEL
Sbjct: 661 GQLMAMSPRIQRTSSGHVSDSEL 682

BLAST of Cucsa.258860 vs. TrEMBL
Match: B9T342_RICCO (Serine-threonine protein kinase, putative OS=Ricinus communis GN=RCOM_0149750 PE=4 SV=1)

HSP 1 Score: 943.7 bits (2438), Expect = 1.2e-271
Identity = 464/688 (67.44%), Postives = 564/688 (81.98%), Query Frame = 1

Query: 1   MEQFRQLGEALGSVKALMLFKDSVHINQRQCCLLLDVLSFAYDSVAEEMKQNLRFEEKHT 60
           ME+FRQ+GE LGS+KALM+F D++ IN+RQCCLL+D+  FAYD++AEEMKQNLRFEEKHT
Sbjct: 1   MERFRQIGEVLGSLKALMVFHDNIQINKRQCCLLVDIFIFAYDTIAEEMKQNLRFEEKHT 60

Query: 61  RWKVLDQPLRDLNRVFKEAEWYIRQCLETKDWWAKVIMLYQNTDCIEFHIHNLLYSITVV 120
           +W++L+QPLR++ R FK+ E YI+QCLETKDWWAK + LYQNTDC++ +IHNLL  I +V
Sbjct: 61  KWRILEQPLREILRTFKDGEGYIKQCLETKDWWAKAVTLYQNTDCVQLYIHNLLTCIPIV 120

Query: 121 VEAIEMAGESSGSDHDELLKKKLINSIKYRREHKDLKIFKWKFGKQYLVTQDFCNRIEAV 180
           +EAIE  GE SG D DE+ KK+L+ S KY+++ KD ++F WKF KQ+L+TQDFC R + V
Sbjct: 121 IEAIETTGEFSGWDQDEIQKKRLVYSNKYQKQWKDPQLFHWKFAKQHLITQDFCERFDVV 180

Query: 181 WNEDRWFLLNKIREKKLMASSKYEQRLTDHLLKNINGSESFNGKLLPSLMLVGSKDYQVR 240
           W EDRW LLNKI+EK ++ S KYE++L D L+KN+   ++ N KLLPS +LVGSKDY VR
Sbjct: 181 WKEDRWILLNKIKEKTVLCSRKYERQLRDLLVKNLEEQDTENAKLLPSSILVGSKDYHVR 240

Query: 241 RRLGVGSQYKEILWLGESFAMRHFFGEIESLIPEISMLLSLSHPNITRFLCGFTDEEKKE 300
           RRLG GSQYKEILWLGES A+RHFFG+I+ L+PEIS LLSLSHPNI+ F CGFTDEEKKE
Sbjct: 241 RRLGNGSQYKEILWLGESLAIRHFFGDIQPLVPEISSLLSLSHPNISYFFCGFTDEEKKE 300

Query: 301 CFLIMELMSRDLSGYVKEICGPRKR-IPFTLPVALDLMLQIARGMEYLHSKKVYHGDLNP 360
           CFL+MELMSRD+  Y+KEICGPRKR +PF+LPVA+D+MLQIARGMEYLHSKK+YHGDLNP
Sbjct: 301 CFLVMELMSRDMCSYIKEICGPRKRSLPFSLPVAVDIMLQIARGMEYLHSKKIYHGDLNP 360

Query: 361 CNILVKPRAYSTDGYVHGQVSGFGLPAVKFKNSSNQNESLPFIWYAPEVLEEQDQSGSAE 420
            NILVKPR+ +T+GYVH +VSGFGL + K KN S QN +L FIWYAPEVLEEQ+Q+GSA 
Sbjct: 361 SNILVKPRS-TTEGYVHVKVSGFGLSSFK-KNPSKQNGTLSFIWYAPEVLEEQEQTGSAP 420

Query: 421 SCKYTEKSDVYSFGMVCFEVLTGKVPFEDSHLQGDKMSRNIRAGERPLFPHSMPKYVTNL 480
           + KYTEKSDVYSFGMVCF +LTGKVPF+DSHLQG+KMSRNIRAGERPLFP + PKYVTNL
Sbjct: 421 NSKYTEKSDVYSFGMVCFGILTGKVPFDDSHLQGEKMSRNIRAGERPLFPLNSPKYVTNL 480

Query: 481 TKRCWQTDPNQRPSFTSICRILRYTKRFVAMNPDYNSQTDPAMPTVDYCDIESGLLRRLP 540
           TKRCWQ DPNQRPSF+SICRILRYTKRF+AMNPDYN + DP MP VDY D++S LLR+ P
Sbjct: 481 TKRCWQADPNQRPSFSSICRILRYTKRFLAMNPDYNRELDPLMPAVDYVDMDSKLLRKYP 540

Query: 541 SCGISDAASPITDIPFQMFAYRVVEKERAGATFKDTSESGSD-ASACGDETASSIDDPFP 600
           S   +D +S I  IPFQMF  RV+EKE+     K+TSESGSD AS  GDE  +  DDPFP
Sbjct: 541 SWDAAD-SSQIAQIPFQMFVCRVIEKEKTRVNQKETSESGSDKASVSGDENMNITDDPFP 600

Query: 601 TPV------ERKLPAPREGSRRLSLTKNNSDV-RLNKLPGTPKGRFSRPPQISPRGRSMR 660
           +P        + L +P   +R+ S++K + DV R ++  GTPKGR SRPPQ++P GR +R
Sbjct: 601 SPTLPSPTGRKFLTSPDTLNRKPSMSKRSPDVIRSSRQAGTPKGR-SRPPQLAPFGRGLR 660

Query: 661 MNSESQL-MAISPKIRRL-SGHASDSEL 678
           MNSESQL M ISP++RR  SGHASDSE+
Sbjct: 661 MNSESQLMMMISPRMRRTSSGHASDSEI 684

BLAST of Cucsa.258860 vs. TAIR10
Match: AT1G64300.1 (AT1G64300.1 Protein kinase family protein)

HSP 1 Score: 712.2 bits (1837), Expect = 3.0e-205
Identity = 376/721 (52.15%), Postives = 489/721 (67.82%), Query Frame = 1

Query: 1   MEQFRQLGEALGSVKALMLFKDSVHINQRQCCLLLDVLSFAYDSVAEEMKQNLRFEEKHT 60
           MEQFRQ+GE LGS+ ALM+ +D + INQRQCCLLLD+ S  +++VAEE++ NL+ EEKHT
Sbjct: 1   MEQFRQIGEVLGSLNALMVLQDDILINQRQCCLLLDIFSLGFNTVAEEIRHNLKLEEKHT 60

Query: 61  RWKVLDQPLRDLNRVFKEAEWYIRQCLETKDWWAKVIMLYQNTDCIEFHIHNLLYSITVV 120
           +W+ L+QPLR+L RVFKE E Y+R C+  KDWW KVI  +QN DC+EFHIHNLL   + V
Sbjct: 61  KWRALEQPLRELYRVFKEGEMYVRNCMSNKDWWGKVINFHQNKDCVEFHIHNLLCYFSAV 120

Query: 121 VEAIEMAGESSGSDHDELLKKKLINSIKYRREHKDLKIFKWKFGKQYLVTQDFCNRIEAV 180
           +EAIE AGE SG D  E+ +++++ S KY RE  D K+F+W+FGKQYLV +D C R E  
Sbjct: 121 IEAIETAGEISGLDPSEMERRRVVFSRKYDREWNDPKLFQWRFGKQYLVPRDICLRFEHS 180

Query: 181 WNEDRWFLLNKIREKKLMAS---SKYEQRLTDHLLKNINGSESFNGKLLPSLMLVGSKDY 240
           W EDRW L+  ++EK+   S    K E+RL D LLK + G E FNGKL PS +LVGSKDY
Sbjct: 181 WREDRWNLVEALQEKRKSKSDEIGKTEKRLADFLLKKLTGLEQFNGKLFPSSILVGSKDY 240

Query: 241 QVRRRLGVGSQYKEILWLGESFAMRHFFGEIESLIPEISMLLSLSHPNITRFLCGFTDEE 300
           QVRRRLG G QYKEI WLG+SF +RHFFG++E L  EIS LLSL H NI ++LCGF DEE
Sbjct: 241 QVRRRLGGGGQYKEIQWLGDSFVLRHFFGDLEPLNAEISSLLSLCHSNILQYLCGFYDEE 300

Query: 301 KKECFLIMELMSRDLSGYVKEICGPRKRIPFTLPVALDLMLQIARGMEYLHSKKVYHGDL 360
           +KEC L+MELM +DL  Y+KE CGPR+R  F++PV +D+MLQIARGMEYLHS +++HGDL
Sbjct: 301 RKECSLVMELMHKDLKSYMKENCGPRRRYLFSVPVVIDIMLQIARGMEYLHSNEIFHGDL 360

Query: 361 NPCNILVKPRAYSTDGYVHGQVSGFGLPAVKFKN----SSNQNESLPFIWYAPEVLE--E 420
           NP NIL+K R++ T+GY H ++SGFGL +VK ++    SS      P IWYAPEVL   E
Sbjct: 361 NPMNILLKERSH-TEGYFHAKISGFGLTSVKNQSFSRASSRPTTPDPVIWYAPEVLAEME 420

Query: 421 QDQSGSAESCKYTEKSDVYSFGMVCFEVLTGKVPFEDSHLQGDKMSRNIRAGERPLFPHS 480
           QD  G+A   K+T K+DVYSF MVCFE++TGKVPFED HLQGDKM++NIR GERPLFP  
Sbjct: 421 QDLKGTAPRSKFTHKADVYSFAMVCFELITGKVPFEDDHLQGDKMAKNIRTGERPLFPFP 480

Query: 481 MPKYVTNLTKRCWQTDPNQRPSFTSICRILRYTKRFVAMNPDYNSQTDPAMPTVDYCDIE 540
            PKY+ +L KRCW ++P+QRP+F+SICRILRY K+F+ +NPD+        P VD  D+E
Sbjct: 481 SPKYLVSLIKRCWHSEPSQRPTFSSICRILRYIKKFLVVNPDH-GHLQIQNPLVDCWDLE 540

Query: 541 SGLLRRLPSCGISDAASPITDIPFQMFAYRVVEKERAGATFKDTSESGSDASACGDETAS 600
           +  L++      S A S +T IPFQ+++YR+ EKE+    F    E  SDA     E+ S
Sbjct: 541 ARFLKKFSMESGSHAGS-VTQIPFQLYSYRIAEKEKMSPNF--NKEENSDAGESVSESVS 600

Query: 601 SIDDPFPT-PVERK---LPAPREGS-----------RRLSLTKNNSDV---RLNKLPG-- 660
            +++P  T P+  K   L A  E S           ++ S  K + D    R N + G  
Sbjct: 601 VVEEPPTTMPMYTKSLCLDAISEYSDTRSVYSEAPIKKTSALKKSGDTIKNRRNSISGLR 660

Query: 661 -------TPKGRFSRPPQISPRGRSM--RMNSESQ--LMAISP----KIRRLSGHASDSE 678
                   P+        +SP GRS   + N +++  L  +SP    + R+L+G ASDSE
Sbjct: 661 SPGSSPIKPRSTPKVSSPLSPFGRSSSNKANKDTRLPLSPMSPLSHGRRRQLTGPASDSE 716

BLAST of Cucsa.258860 vs. TAIR10
Match: AT5G41730.1 (AT5G41730.1 Protein kinase family protein)

HSP 1 Score: 699.5 bits (1804), Expect = 2.0e-201
Identity = 364/719 (50.63%), Postives = 485/719 (67.45%), Query Frame = 1

Query: 1   MEQFRQLGEALGSVKALMLFKDSVHINQRQCCLLLDVLSFAYDSVAEEMKQNLRFEEKHT 60
           MEQFRQ+GE LGS+ ALM+ +D + INQRQCCLLL++ S A+++VAEE++QNL+ EEKHT
Sbjct: 1   MEQFRQIGEVLGSLNALMVLQDDILINQRQCCLLLELFSLAFNTVAEEIRQNLKLEEKHT 60

Query: 61  RWKVLDQPLRDLNRVFKEAEWYIRQCLETKDWWAKVIMLYQNTDCIEFHIHNLLYSITVV 120
           +W+ L+QPLR+L RVFKE E Y++ C++  DWW KVI L+QN DC+EFHIHNL    + V
Sbjct: 61  KWRALEQPLRELYRVFKEGELYVKHCMDNSDWWGKVINLHQNKDCVEFHIHNLFCYFSAV 120

Query: 121 VEAIEMAGESSGSDHDELLKKKLINSIKYRREHKDLKIFKWKFGKQYLVTQDFCNRIEAV 180
           VEAIE AGE SG D  E+ +++++ S KY RE  D K+F+W+FGKQYL+++D C+R E  
Sbjct: 121 VEAIEAAGEISGLDPSEMERRRVVFSRKYDREWNDPKMFQWRFGKQYLLSRDICSRFEHS 180

Query: 181 WNEDRWFLLNKIREKKLMAS---SKYEQRLTDHLLKNINGSESFNGKLLPSLMLVGSKDY 240
           W EDRW L+  ++EK+   S    K E+RL D LLK + G E FNGKL PS +L+GSKDY
Sbjct: 181 WREDRWNLVEALQEKRKSDSDDIGKTEKRLADLLLKKLTGLEQFNGKLFPSSILLGSKDY 240

Query: 241 QVRRRLGVGSQYKEILWLGESFAMRHFFGEIESLIPEISMLLSLSHPNITRFLCGFTDEE 300
           QV++RL    QYKEI WLG+SFA+RHFF ++E L  EIS LL+L H NI ++LCGF DEE
Sbjct: 241 QVKKRLDADGQYKEIQWLGDSFALRHFFSDLEPLSSEISSLLALCHSNILQYLCGFYDEE 300

Query: 301 KKECFLIMELMSRDLSGYVKEICGPRKRIPFTLPVALDLMLQIARGMEYLHSKKVYHGDL 360
           +KECFL+MELM +DL  Y+KE CGPR+R  F++PV +D+MLQIARGMEYLH   ++HGDL
Sbjct: 301 RKECFLVMELMHKDLQSYMKENCGPRRRYLFSIPVVIDIMLQIARGMEYLHGNDIFHGDL 360

Query: 361 NPCNILVKPRAYSTDGYVHGQVSGFGLPA-VKFKNSSNQNESLPFIWYAPEVLE--EQDQ 420
           NP NI +K R++ T+GY H ++ GFGL + VK ++SS      P IWYAPEVL   EQD 
Sbjct: 361 NPMNIHLKERSH-TEGYFHAKICGFGLSSVVKAQSSSKPGTPDPVIWYAPEVLAEMEQDL 420

Query: 421 SGSAESCKYTEKSDVYSFGMVCFEVLTGKVPFEDSHLQGDKMSRNIRAGERPLFPHSMPK 480
           +G     K T K+DVYSF MVCFE++TGKVPFEDSHLQG+ M+ NIR GERPLFP   PK
Sbjct: 421 NGKTPKSKLTHKADVYSFAMVCFELITGKVPFEDSHLQGEPMTINIRMGERPLFPFPSPK 480

Query: 481 YVTNLTKRCWQTDPNQRPSFTSICRILRYTKRFVAMNPDYNSQTDPAMPTVDYCDIESGL 540
           Y+ +L KRCW ++P+QRP+F+SICRILRY K+F+ +NPD+        P VD  D+E+  
Sbjct: 481 YLVSLIKRCWHSEPSQRPNFSSICRILRYIKKFLVVNPDH-GHPQMQTPLVDCWDLEARF 540

Query: 541 LRRLPSCGISDAASPITDIPFQMFAYRVVEKERAGATFKDTSESGSDASACGDETASSID 600
           LR+ P    S  AS +  IPFQ+++YRV+EKE+     K++SE+         E+ S ++
Sbjct: 541 LRKFPGDAGSHTAS-VNQIPFQLYSYRVLEKEKMNPNSKESSETSE------SESVSVVE 600

Query: 601 DPFPTPVERK-----LPAPREGSRRLS------------LTKNNSDVRLNKLP------- 660
           DP    + R      L    E S   S            L K+    +L + P       
Sbjct: 601 DPPNAMITRDTKSLCLDTISEYSDTRSVYSEAPMKKVSALKKSGEMAKLRRSPSLGSEKL 660

Query: 661 ---GTPKGRFSRPPQISPR---GRSMRMNSESQL-----MAISPKIRR-LSGHASDSEL 678
              GT   +    P++SP    GRS++   +++L       ++P IRR  +GHASDSEL
Sbjct: 661 RSAGTSTVKARSSPKVSPLSPFGRSIKARKDNRLPLSPMSPLTPGIRRQQTGHASDSEL 710

BLAST of Cucsa.258860 vs. TAIR10
Match: AT1G01450.1 (AT1G01450.1 Protein kinase superfamily protein)

HSP 1 Score: 498.8 bits (1283), Expect = 5.2e-141
Identity = 255/412 (61.89%), Postives = 327/412 (79.37%), Query Frame = 1

Query: 207 LTDHLLKNI-NGSESFNGKLLPSLMLVGSKDYQVRRRLGVGSQYKEILWLGESFAMRHFF 266
           + D LLK++ +G+ES   KL PS +L  +KDYQV++RLG GSQYKEI WLGESFA+RHFF
Sbjct: 1   MADFLLKHLGDGNES--PKLFPSSLLDNTKDYQVKKRLGNGSQYKEITWLGESFALRHFF 60

Query: 267 GEIESLIPEISMLLSLSHPNITRFLCGFTDEEKKECFLIMELMSRDLSGYVKEICGPRKR 326
           G+I++L+P+I+ LLSLSHPNI  +LCGFTDEEKKECFL+MELM + L  ++KE+CGPRK+
Sbjct: 61  GDIDALLPQITPLLSLSHPNIVYYLCGFTDEEKKECFLVMELMRKTLGMHIKEVCGPRKK 120

Query: 327 IPFTLPVALDLMLQIARGMEYLHSKKVYHGDLNPCNILVKPRA-YSTDGYVHGQVSGFGL 386
              +LPVA+DLMLQIA GMEYLHSK++YHG+LNP NILVKPR+  S DGY+ G++ GFGL
Sbjct: 121 NTLSLPVAVDLMLQIALGMEYLHSKRIYHGELNPSNILVKPRSNQSGDGYLLGKIFGFGL 180

Query: 387 PAVKFKNS-----SNQNESLPFIWYAPEVLEEQDQSGSAESCKYTEKSDVYSFGMVCFEV 446
            +VK  +S     ++QNE+ PFIWY+PEVLEEQ+QSG+A S KY++KSDVYSFGMV FE+
Sbjct: 181 NSVKGFSSKSASLTSQNENFPFIWYSPEVLEEQEQSGTAGSLKYSDKSDVYSFGMVSFEL 240

Query: 447 LTGKVPFEDSHLQGDKMSRNIRAGERPLFPHSMPKYVTNLTKRCWQTDPNQRPSFTSICR 506
           LTGKVPFEDSHLQGDKMSRNIRAGERPLFP + PK++TNLTKRCW  DPNQRP+F+SI R
Sbjct: 241 LTGKVPFEDSHLQGDKMSRNIRAGERPLFPFNSPKFITNLTKRCWHADPNQRPTFSSISR 300

Query: 507 ILRYTKRFVAMNPD--YNSQTDPAM-PTVDYCDIESGLLRRLPSCGISDAASPITDIPFQ 566
           ILRY KRF+A+NP+   +SQ DP++ PTVDYC+IE+ LL++L     S   + ++ +PFQ
Sbjct: 301 ILRYIKRFLALNPECYSSSQQDPSIAPTVDYCEIETKLLQKL--SWESTELTKVSQVPFQ 360

Query: 567 MFAYRVVEKERA--GATFKDTSESGSDASACG-DETASSIDDPFPTPVERKL 606
           MFAYRVVE+ +       ++ SESGS+ ++C  DE  +  D+      ER+L
Sbjct: 361 MFAYRVVERAKTCEKDNLREPSESGSEWASCSEDEGGAGSDEQLSYAKERRL 408

BLAST of Cucsa.258860 vs. TAIR10
Match: AT3G46930.1 (AT3G46930.1 Protein kinase superfamily protein)

HSP 1 Score: 108.2 bits (269), Expect = 2.0e-23
Identity = 71/260 (27.31%), Postives = 121/260 (46.54%), Query Frame = 1

Query: 269 ESLIPEISMLLSLSHPNITRFLCGFTDEEKKECFLIMELMSRDLSGYVKEICGPRKRIPF 328
           +  I E ++L  LSHPN+ +F+   T      C +   +    L  Y+ ++   +K +P 
Sbjct: 207 KEFIVEATLLSRLSHPNVVKFVGVNTGN----CIITEYVPRGSLRSYLHKL--EQKSLP- 266

Query: 329 TLPVALDLMLQIARGMEYLHSKKVYHGDLNPCNILVKPRAYSTDGYVHGQVSGFGLPAVK 388
            L   +D  L IA+GMEY+HS+++ H DL P N+L+       D   H +++ FG+   +
Sbjct: 267 -LEQLIDFGLDIAKGMEYIHSREIVHQDLKPENVLI-------DNDFHLKIADFGIACEE 326

Query: 389 FKNSSNQNESLPFIWYAPEVLEEQDQSGSAESCKYTEKSDVYSFGMVCFEVLTGKVPFED 448
                  +    + W APEVL+            +  K DVYSFG++ +E++ G +P+E+
Sbjct: 327 EYCDVLGDNIGTYRWMAPEVLKR---------IPHGRKCDVYSFGLLLWEMVAGALPYEE 386

Query: 449 SHLQGDKMSRNIRAGERPLFPHSMPKYVTNLTKRCWQTDPNQRPSFTSICRILRYTKRFV 508
                      I    RP+ P   P  +  L +RCW +  ++RP F  I ++L + K+ +
Sbjct: 387 MKFAEQIAYAVIYKKIRPVIPTDCPAAMKELIERCWSSQTDKRPEFWQIVKVLEHFKKSL 442

Query: 509 AMNPDYNSQTDPAMPTVDYC 529
                 N       P +  C
Sbjct: 447 TSEGKLNLLPSQICPELKKC 442

BLAST of Cucsa.258860 vs. TAIR10
Match: AT1G14000.1 (AT1G14000.1 VH1-interacting kinase)

HSP 1 Score: 106.3 bits (264), Expect = 7.5e-23
Identity = 89/288 (30.90%), Postives = 137/288 (47.57%), Query Frame = 1

Query: 236 DYQVRRRLGVGS--QYKEILWLGESFAMRHFFGE-------IESLIPEISMLLSLSHPNI 295
           D+     +G GS  +  +  W G   A++            I+    E+ +L+ L HPNI
Sbjct: 161 DFSNAAMIGKGSFGEIVKAYWRGTPVAVKRILPSLSDDRLVIQDFRHEVDLLVKLRHPNI 220

Query: 296 TRFLCGFTDEEKKECFLIME-LMSRDLSGYVKEICGPRKRIPFTLPVALDLMLQIARGME 355
            +FL   T  E+K   LI E L   DL  Y+KE  G       T   A++  L IARGM 
Sbjct: 221 VQFLGAVT--ERKPLMLITEYLRGGDLHQYLKEKGG------LTPTTAVNFALDIARGMT 280

Query: 356 YLHSKK--VYHGDLNPCNILVKPRAYSTDGYVHGQVSGFGLPA-VKFKNSSN----QNES 415
           YLH++   + H DL P N+L+   +       H +V  FGL   +K +NS +      E+
Sbjct: 281 YLHNEPNVIIHRDLKPRNVLLVNSSAD-----HLKVGDFGLSKLIKVQNSHDVYKMTGET 340

Query: 416 LPFIWYAPEVLEEQDQSGSAESCKYTEKSDVYSFGMVCFEVLTGKVPFEDSHLQGDKMSR 475
             + + APEV + +         +Y +K DV+SF M+ +E+L G+ PF  ++ +  + ++
Sbjct: 341 GSYRYMAPEVFKHR---------RYDKKVDVFSFAMILYEMLEGEPPF--ANHEPYEAAK 400

Query: 476 NIRAGERPLF-PHSMPKYVTNLTKRCWQTDPNQRPSFTSICRILRYTK 506
           ++  G RP F        +  L  +CW  D NQRPSF  I + L   K
Sbjct: 401 HVSDGHRPTFRSKGCTPDLRELIVKCWDADMNQRPSFLDILKRLEKIK 424

BLAST of Cucsa.258860 vs. NCBI nr
Match: gi|449433053|ref|XP_004134312.1| (PREDICTED: light-sensor Protein kinase-like [Cucumis sativus])

HSP 1 Score: 1382.5 bits (3577), Expect = 0.0e+00
Identity = 678/678 (100.00%), Postives = 678/678 (100.00%), Query Frame = 1

Query: 1   MEQFRQLGEALGSVKALMLFKDSVHINQRQCCLLLDVLSFAYDSVAEEMKQNLRFEEKHT 60
           MEQFRQLGEALGSVKALMLFKDSVHINQRQCCLLLDVLSFAYDSVAEEMKQNLRFEEKHT
Sbjct: 1   MEQFRQLGEALGSVKALMLFKDSVHINQRQCCLLLDVLSFAYDSVAEEMKQNLRFEEKHT 60

Query: 61  RWKVLDQPLRDLNRVFKEAEWYIRQCLETKDWWAKVIMLYQNTDCIEFHIHNLLYSITVV 120
           RWKVLDQPLRDLNRVFKEAEWYIRQCLETKDWWAKVIMLYQNTDCIEFHIHNLLYSITVV
Sbjct: 61  RWKVLDQPLRDLNRVFKEAEWYIRQCLETKDWWAKVIMLYQNTDCIEFHIHNLLYSITVV 120

Query: 121 VEAIEMAGESSGSDHDELLKKKLINSIKYRREHKDLKIFKWKFGKQYLVTQDFCNRIEAV 180
           VEAIEMAGESSGSDHDELLKKKLINSIKYRREHKDLKIFKWKFGKQYLVTQDFCNRIEAV
Sbjct: 121 VEAIEMAGESSGSDHDELLKKKLINSIKYRREHKDLKIFKWKFGKQYLVTQDFCNRIEAV 180

Query: 181 WNEDRWFLLNKIREKKLMASSKYEQRLTDHLLKNINGSESFNGKLLPSLMLVGSKDYQVR 240
           WNEDRWFLLNKIREKKLMASSKYEQRLTDHLLKNINGSESFNGKLLPSLMLVGSKDYQVR
Sbjct: 181 WNEDRWFLLNKIREKKLMASSKYEQRLTDHLLKNINGSESFNGKLLPSLMLVGSKDYQVR 240

Query: 241 RRLGVGSQYKEILWLGESFAMRHFFGEIESLIPEISMLLSLSHPNITRFLCGFTDEEKKE 300
           RRLGVGSQYKEILWLGESFAMRHFFGEIESLIPEISMLLSLSHPNITRFLCGFTDEEKKE
Sbjct: 241 RRLGVGSQYKEILWLGESFAMRHFFGEIESLIPEISMLLSLSHPNITRFLCGFTDEEKKE 300

Query: 301 CFLIMELMSRDLSGYVKEICGPRKRIPFTLPVALDLMLQIARGMEYLHSKKVYHGDLNPC 360
           CFLIMELMSRDLSGYVKEICGPRKRIPFTLPVALDLMLQIARGMEYLHSKKVYHGDLNPC
Sbjct: 301 CFLIMELMSRDLSGYVKEICGPRKRIPFTLPVALDLMLQIARGMEYLHSKKVYHGDLNPC 360

Query: 361 NILVKPRAYSTDGYVHGQVSGFGLPAVKFKNSSNQNESLPFIWYAPEVLEEQDQSGSAES 420
           NILVKPRAYSTDGYVHGQVSGFGLPAVKFKNSSNQNESLPFIWYAPEVLEEQDQSGSAES
Sbjct: 361 NILVKPRAYSTDGYVHGQVSGFGLPAVKFKNSSNQNESLPFIWYAPEVLEEQDQSGSAES 420

Query: 421 CKYTEKSDVYSFGMVCFEVLTGKVPFEDSHLQGDKMSRNIRAGERPLFPHSMPKYVTNLT 480
           CKYTEKSDVYSFGMVCFEVLTGKVPFEDSHLQGDKMSRNIRAGERPLFPHSMPKYVTNLT
Sbjct: 421 CKYTEKSDVYSFGMVCFEVLTGKVPFEDSHLQGDKMSRNIRAGERPLFPHSMPKYVTNLT 480

Query: 481 KRCWQTDPNQRPSFTSICRILRYTKRFVAMNPDYNSQTDPAMPTVDYCDIESGLLRRLPS 540
           KRCWQTDPNQRPSFTSICRILRYTKRFVAMNPDYNSQTDPAMPTVDYCDIESGLLRRLPS
Sbjct: 481 KRCWQTDPNQRPSFTSICRILRYTKRFVAMNPDYNSQTDPAMPTVDYCDIESGLLRRLPS 540

Query: 541 CGISDAASPITDIPFQMFAYRVVEKERAGATFKDTSESGSDASACGDETASSIDDPFPTP 600
           CGISDAASPITDIPFQMFAYRVVEKERAGATFKDTSESGSDASACGDETASSIDDPFPTP
Sbjct: 541 CGISDAASPITDIPFQMFAYRVVEKERAGATFKDTSESGSDASACGDETASSIDDPFPTP 600

Query: 601 VERKLPAPREGSRRLSLTKNNSDVRLNKLPGTPKGRFSRPPQISPRGRSMRMNSESQLMA 660
           VERKLPAPREGSRRLSLTKNNSDVRLNKLPGTPKGRFSRPPQISPRGRSMRMNSESQLMA
Sbjct: 601 VERKLPAPREGSRRLSLTKNNSDVRLNKLPGTPKGRFSRPPQISPRGRSMRMNSESQLMA 660

Query: 661 ISPKIRRLSGHASDSELP 679
           ISPKIRRLSGHASDSELP
Sbjct: 661 ISPKIRRLSGHASDSELP 678

BLAST of Cucsa.258860 vs. NCBI nr
Match: gi|659075106|ref|XP_008437968.1| (PREDICTED: mitogen-activated protein kinase kinase kinase 7-like [Cucumis melo])

HSP 1 Score: 1332.8 bits (3448), Expect = 0.0e+00
Identity = 649/678 (95.72%), Postives = 662/678 (97.64%), Query Frame = 1

Query: 1   MEQFRQLGEALGSVKALMLFKDSVHINQRQCCLLLDVLSFAYDSVAEEMKQNLRFEEKHT 60
           MEQFRQLGEALGSVKALMLFKDSVHINQRQCCLLLDVLSFAYDSVAEEMKQNLRFEEKHT
Sbjct: 1   MEQFRQLGEALGSVKALMLFKDSVHINQRQCCLLLDVLSFAYDSVAEEMKQNLRFEEKHT 60

Query: 61  RWKVLDQPLRDLNRVFKEAEWYIRQCLETKDWWAKVIMLYQNTDCIEFHIHNLLYSITVV 120
           RWKVLDQPLRDLNRVFKEAEWYIRQCLETKDWWAKVI LYQNTDCIEFHIHNLLYSITVV
Sbjct: 61  RWKVLDQPLRDLNRVFKEAEWYIRQCLETKDWWAKVITLYQNTDCIEFHIHNLLYSITVV 120

Query: 121 VEAIEMAGESSGSDHDELLKKKLINSIKYRREHKDLKIFKWKFGKQYLVTQDFCNRIEAV 180
           VEAIE AGE+SGSDHDELLKK+LINSIKYRREHKDLKIFKWKFGKQYL+T+DFCNRI+AV
Sbjct: 121 VEAIETAGENSGSDHDELLKKRLINSIKYRREHKDLKIFKWKFGKQYLITRDFCNRIDAV 180

Query: 181 WNEDRWFLLNKIREKKLMASSKYEQRLTDHLLKNINGSESFNGKLLPSLMLVGSKDYQVR 240
           WNEDRWFLLNKIREKK  ASSKYEQRLTDHL+KNINGSES NGKLLPS MLVGSKDYQVR
Sbjct: 181 WNEDRWFLLNKIREKKSTASSKYEQRLTDHLMKNINGSESLNGKLLPSSMLVGSKDYQVR 240

Query: 241 RRLGVGSQYKEILWLGESFAMRHFFGEIESLIPEISMLLSLSHPNITRFLCGFTDEEKKE 300
           RRLG GSQYKEILWLGESFAMRHFFGEIESLIPEIS+LLSLSHPNITRFLCGFTDEEKKE
Sbjct: 241 RRLGGGSQYKEILWLGESFAMRHFFGEIESLIPEISILLSLSHPNITRFLCGFTDEEKKE 300

Query: 301 CFLIMELMSRDLSGYVKEICGPRKRIPFTLPVALDLMLQIARGMEYLHSKKVYHGDLNPC 360
           CFLIMELMSRDLS YVKEICGPRKRIPF LPVALDLMLQIARGMEYLHSKKVYHGDLNPC
Sbjct: 301 CFLIMELMSRDLSSYVKEICGPRKRIPFPLPVALDLMLQIARGMEYLHSKKVYHGDLNPC 360

Query: 361 NILVKPRAYSTDGYVHGQVSGFGLPAVKFKNSSNQNESLPFIWYAPEVLEEQDQSGSAES 420
           NILVKPRAYSTDGYVHGQVSGFGLPAVKFKNSSNQNESLPFIWYAPEVLEEQDQSGSAES
Sbjct: 361 NILVKPRAYSTDGYVHGQVSGFGLPAVKFKNSSNQNESLPFIWYAPEVLEEQDQSGSAES 420

Query: 421 CKYTEKSDVYSFGMVCFEVLTGKVPFEDSHLQGDKMSRNIRAGERPLFPHSMPKYVTNLT 480
            KYTEKSDVYSFGMVCFEVLTGKVPFEDSHLQGDKMSRNIRAGERPLFPHSMPKYVTNLT
Sbjct: 421 SKYTEKSDVYSFGMVCFEVLTGKVPFEDSHLQGDKMSRNIRAGERPLFPHSMPKYVTNLT 480

Query: 481 KRCWQTDPNQRPSFTSICRILRYTKRFVAMNPDYNSQTDPAMPTVDYCDIESGLLRRLPS 540
           KRCW TDPNQRPSFTSICRILRYTKRFVAMNPDYNSQTDPAMPTVDYCDIESGLLRRLP 
Sbjct: 481 KRCWHTDPNQRPSFTSICRILRYTKRFVAMNPDYNSQTDPAMPTVDYCDIESGLLRRLPP 540

Query: 541 CGISDAASPITDIPFQMFAYRVVEKERAGATFKDTSESGSDASACGDETASSIDDPFPTP 600
           CGISDAASPITDIPFQMFAYR+VEKERAGATFKDTSESGSDASACGDET SSIDDPFPT 
Sbjct: 541 CGISDAASPITDIPFQMFAYRIVEKERAGATFKDTSESGSDASACGDETGSSIDDPFPTS 600

Query: 601 VERKLPAPREGSRRLSLTKNNSDVRLNKLPGTPKGRFSRPPQISPRGRSMRMNSESQLMA 660
           VE+KLPAPREGSRRLSLTKNNSD++LNKLPGTPKGRFSRPPQISPRGRS+RMNSESQLM 
Sbjct: 601 VEKKLPAPREGSRRLSLTKNNSDIKLNKLPGTPKGRFSRPPQISPRGRSLRMNSESQLMT 660

Query: 661 ISPKIRRLSGHASDSELP 679
           ISPK+RR SGHASDSELP
Sbjct: 661 ISPKVRRSSGHASDSELP 678

BLAST of Cucsa.258860 vs. NCBI nr
Match: gi|596031780|ref|XP_007219478.1| (hypothetical protein PRUPE_ppa021542mg [Prunus persica])

HSP 1 Score: 974.9 bits (2519), Expect = 7.0e-281
Identity = 485/692 (70.09%), Postives = 573/692 (82.80%), Query Frame = 1

Query: 1   MEQFRQLGEALGSVKALMLFKDSVHINQRQCCLLLDVLSFAYDSVAEEMKQNLRFEEKHT 60
           MEQFR++GE LGS+KALM+F+D++ INQRQC LLLD+ S AY+S+AEEMK NLRFEEKHT
Sbjct: 1   MEQFRKIGEDLGSLKALMVFQDNIQINQRQCFLLLDIFSSAYESIAEEMKYNLRFEEKHT 60

Query: 61  RWKVLDQPLRDLNRVFKEAEWYIRQCLETKDWWAKVIMLYQNTDCIEFHIHNLLYSITVV 120
           +W+VL+QPLR+L+R+FKE E YIR CLETKDWWAK I LYQN+DCIEFHIHNLL  + +V
Sbjct: 61  KWRVLEQPLRELHRIFKEGEAYIRHCLETKDWWAKAITLYQNSDCIEFHIHNLLSCMPIV 120

Query: 121 VEAIEMAGESSGSDHDELLKKKLINSIKYRREHKDLKIFKWKFGKQYLVTQDFCNRIEAV 180
           +EAI++AGE SG   DE+ +KK + + KY+ +++D K+FKW+FGKQYL+TQDFCNR +  
Sbjct: 121 IEAIDIAGEISGWGQDEIQRKKTVFADKYKDDYRDWKLFKWRFGKQYLITQDFCNRFDTT 180

Query: 181 WNEDRWFLLNKIREKKLMASSKYEQRLTDHLLKNINGSES--FNGKLLPSLMLVGSKDYQ 240
           W EDRW LL+KIREKKL  S+KY +RL D L K+++GSES   NGKLLPS +LVGSKDYQ
Sbjct: 181 WKEDRWTLLHKIREKKLSGSTKYGKRLIDLLFKSLDGSESQPLNGKLLPSSILVGSKDYQ 240

Query: 241 VRRRLGVGSQYKEILWLGESFAMRHFFGEIESLIPEISMLLSLSHPNITRFLCGFTDEEK 300
           VRRRLG GSQYKEILWLGESFA RHFFG+IE L+PEIS LLSLSHPNI  FLCGFTDEEK
Sbjct: 241 VRRRLGGGSQYKEILWLGESFASRHFFGDIEPLLPEISSLLSLSHPNIVHFLCGFTDEEK 300

Query: 301 KECFLIMELMSRDLSGYVKEICGPRKRIPFTLPVALDLMLQIARGMEYLHSKKVYHGDLN 360
           KECFLIMELMSRDL  ++KEICGPRKR+PF+LPVA+DLMLQIARGMEYLHSKK+YHG+LN
Sbjct: 301 KECFLIMELMSRDLCSHIKEICGPRKRLPFSLPVAVDLMLQIARGMEYLHSKKIYHGELN 360

Query: 361 PCNILVKPRAYSTDGYVHGQVSGFGLPAVKF---KNSSNQNESLPFIWYAPEVLEEQDQS 420
           PCNILVK R  STDGY+  +VSGFGL + K    KN SNQN SLPFIWYAPEVLEE +Q+
Sbjct: 361 PCNILVKARGISTDGYLQAKVSGFGLTSAKSPSQKNLSNQNGSLPFIWYAPEVLEEHEQT 420

Query: 421 GSAESCKYTEKSDVYSFGMVCFEVLTGKVPFEDSHLQGDKMSRNIRAGERPLFPHSMPKY 480
            S+E  KYTEKSDVYSFGMVCFE+LTGKVPFEDSHLQG+KMSRNIRAGERPLFP    +Y
Sbjct: 421 KSSEK-KYTEKSDVYSFGMVCFELLTGKVPFEDSHLQGEKMSRNIRAGERPLFPFYSLRY 480

Query: 481 VTNLTKRCWQTDPNQRPSFTSICRILRYTKRFVAMNPDYNSQTDPAMPTVDYCDIESGLL 540
           VTNLTK+CW +DPN RPSF+SICRILRY KRF+AMNPDYNSQ DP +P VDYC+IESG+L
Sbjct: 481 VTNLTKKCWHSDPNLRPSFSSICRILRYIKRFLAMNPDYNSQLDPPVPMVDYCEIESGIL 540

Query: 541 RRLPSCGISDAASPITDIPFQMFAYRVVEKERAGATFKDTSESGSD-ASACGDETASSID 600
           R++PS   S    P++ IPFQMF YR+ E+ER   T KDTSESGSD AS CGDE  ++ D
Sbjct: 541 RKIPSWK-SCEQPPVSQIPFQMFVYRIAERER--TTLKDTSESGSDGASICGDEMVTTPD 600

Query: 601 DPFPTPVERK-LPAPREGSRRLSLTKNNSDVRLNKLP-----GTPKGRFSRPPQISPRGR 660
           +PFP   ERK L +P    ++L   K +SDV+ N+LP     GTP+GR  RPPQ+SP GR
Sbjct: 601 EPFPPAPERKCLTSPDSMKKKLPFLKKSSDVKANRLPGFGYTGTPRGRSVRPPQMSPCGR 660

Query: 661 --SMRMNSESQLMAISPKIRRL-SGHASDSEL 678
             S+RM+SESQLMA++P+IRR  SGHASDSEL
Sbjct: 661 SISLRMSSESQLMAMTPRIRRTSSGHASDSEL 688

BLAST of Cucsa.258860 vs. NCBI nr
Match: gi|1009143663|ref|XP_015889383.1| (PREDICTED: LOW QUALITY PROTEIN: probable serine/threonine-protein kinase gdt4 [Ziziphus jujuba])

HSP 1 Score: 971.5 bits (2510), Expect = 7.8e-280
Identity = 473/686 (68.95%), Postives = 564/686 (82.22%), Query Frame = 1

Query: 1   MEQFRQLGEALGSVKALMLFKDSVHINQRQCCLLLDVLSFAYDSVAEEMKQNLRFEEKHT 60
           MEQFR +GE LGS+KA+M+F+ ++ INQ+QC  L D+  FAY+S+AEEM+ NLRFEEKHT
Sbjct: 1   MEQFRHIGEVLGSLKAIMVFRGNIQINQKQCFFLFDIFCFAYESIAEEMEHNLRFEEKHT 60

Query: 61  RWKVLDQPLRDLNRVFKEAEWYIRQCLETKDWWAKVIMLYQNTDCIEFHIHNLLYSITVV 120
           +WK+L+QPLRDL R+F+E E YIRQCLETKDWWAK I LYQN+DC+EFHIHNLL  +  V
Sbjct: 61  KWKILEQPLRDLYRIFREGETYIRQCLETKDWWAKAITLYQNSDCVEFHIHNLLCCMPNV 120

Query: 121 VEAIEMAGESSGSDHDELLKKKLINSIKYRREHKDLKIFKWKFGKQYLVTQDFCNRIEAV 180
           +EAIE AGE SG D D + KKK + S KYRRE++D K+F+W+FGKQYL+TQDFCNRI+ V
Sbjct: 121 IEAIETAGEISGLDQDVMQKKKHVYSNKYRREYRDWKLFQWRFGKQYLITQDFCNRIDTV 180

Query: 181 WNEDRWFLLNKIREKKLMASSKYEQRLTDHLLKNINGSESFNGKLLPSLMLVGSKDYQVR 240
           W EDRW LLNKIREKK+  S+KYEQRL D   K+++G E  NG+L P  +LVGSKDY VR
Sbjct: 181 WKEDRWTLLNKIREKKISGSTKYEQRLIDLFFKSLDGLEPLNGRLFPCSILVGSKDYHVR 240

Query: 241 RRLGVGSQYKEILWLGESFAMRHFFGEIESLI--PEISMLLSLSHPNITRFLCGFTDEEK 300
           RRLG GSQYKEILWLGESF +RHFFG+IE+L    EI+ LLSLSHPNI  +LCGFTDEEK
Sbjct: 241 RRLGSGSQYKEILWLGESFVLRHFFGDIETLSRQQEITSLLSLSHPNIMHYLCGFTDEEK 300

Query: 301 KECFLIMELMSRDLSGYVKEICGPRKRIPFTLPVALDLMLQIARGMEYLHSKKVYHGDLN 360
           KECFLI ELM++DLS Y KEICGPRK+IPF+LPVA+DLMLQIARGMEYLHSK+++HG LN
Sbjct: 301 KECFLITELMNKDLSSYNKEICGPRKKIPFSLPVAVDLMLQIARGMEYLHSKQIFHGHLN 360

Query: 361 PCNILVKPRAYSTDGYVHGQVSGFGLPAVK---FKNSSNQNESLPFIWYAPEVLEEQDQS 420
           P NILV+PR  S++G +H +VSGFGL +++    K+ SN N +LPFIWY+PEVLEEQ+Q+
Sbjct: 361 PSNILVRPRGISSEGNLHAKVSGFGLNSIRRFTTKSPSNHNGTLPFIWYSPEVLEEQEQT 420

Query: 421 GSAESCKYTEKSDVYSFGMVCFEVLTGKVPFEDSHLQGDKMSRNIRAGERPLFPHSMPKY 480
           G+AE+ KYTEKSDVYSFGMVCFE+LTGKVPFEDSHLQGDKMSRNIRAGERPLF    PKY
Sbjct: 421 GAAENSKYTEKSDVYSFGMVCFELLTGKVPFEDSHLQGDKMSRNIRAGERPLFTFQSPKY 480

Query: 481 VTNLTKRCWQTDPNQRPSFTSICRILRYTKRFVAMNPDYNSQTDPAMPTVDYCDIESGLL 540
           VTNLTK+CW TDPNQRPSFTSICRILRY KRF+AMNPD+N Q DP +P VDYCDIES LL
Sbjct: 481 VTNLTKKCWHTDPNQRPSFTSICRILRYIKRFLAMNPDHNIQLDPPVPHVDYCDIESRLL 540

Query: 541 RRLPSCGISDAASPITDIPFQMFAYRVVEKERAGATFKDTSESGSD-ASACGDETASSID 600
           R  PSC      S ++ IPFQMF YR++EKE+  A+ KD SESGSD AS CGD+  +++D
Sbjct: 541 RNFPSC-----QSSVSQIPFQMFVYRILEKEKISASLKDNSESGSDGASVCGDDHITTVD 600

Query: 601 DPFP-TPV-ERKLPAPREGSRRLSLTKNNSDVRLNKLPGTPKGRFSRPPQISPRGRSMRM 660
           DPFP +P+ ERK  A  + + R    K + D RLNKLPGTP+GR  RPPQ+SP GRSMRM
Sbjct: 601 DPFPSSPIPERKSLASLDVTNRKLSIKRSPDSRLNKLPGTPRGRSVRPPQMSPCGRSMRM 660

Query: 661 NSESQLMAISPKIRRL-SGHASDSEL 678
           +SESQLMA+SP+IRR  SGHASDSE+
Sbjct: 661 SSESQLMAMSPRIRRTSSGHASDSEI 681

BLAST of Cucsa.258860 vs. NCBI nr
Match: gi|657968710|ref|XP_008376057.1| (PREDICTED: mitogen-activated protein kinase kinase kinase 7-like [Malus domestica])

HSP 1 Score: 959.9 bits (2480), Expect = 2.3e-276
Identity = 476/686 (69.39%), Postives = 568/686 (82.80%), Query Frame = 1

Query: 1   MEQFRQLGEALGSVKALMLFKDSVHINQRQCCLLLDVLSFAYDSVAEEMKQNLRFEEKHT 60
           MEQFR++GE +GS+KALM+F+D++ INQRQC LLLD+ S AY+S+AEE + NLRF+EKH 
Sbjct: 1   MEQFRRIGEDVGSLKALMVFQDNIQINQRQCFLLLDIFSSAYESIAEEXRHNLRFDEKHM 60

Query: 61  RWKVLDQPLRDLNRVFKEAEWYIRQCLETKDWWAKVIMLYQNTDCIEFHIHNLLYSITVV 120
           +W+VL+QPLR+L+R+FK+AE YIRQCL+T DWWAK I LYQN+DC+EFHIHNLL  + +V
Sbjct: 61  KWRVLEQPLRELHRIFKDAEAYIRQCLDTXDWWAKAITLYQNSDCVEFHIHNLLACVPIV 120

Query: 121 VEAIEMAGESSGSDHDELLKKKLINSIKYRREHKDLKIFKWKFGKQYLVTQDFCNRIEAV 180
           +EAI++AGE SG D DE+ +KK + + KY+++++D K+FKW+FGKQYL+TQDFCNR E+ 
Sbjct: 121 IEAIDIAGEISGLDQDEIQRKKTMFADKYKQDYRDWKLFKWRFGKQYLITQDFCNRFESA 180

Query: 181 WNEDRWFLLNKIREKKLMASSKYEQRLTDHLLKNINGSESFN--GKLLPSLMLVGSKDYQ 240
            NEDRW LL+KI EKKL  S+KY +RL D L K+ +GSES N  GKLLPS +LVGSKDYQ
Sbjct: 181 GNEDRWTLLHKISEKKLSGSTKYGKRLID-LFKSADGSESQNLKGKLLPSSVLVGSKDYQ 240

Query: 241 VRRRLGVGSQYKEILWLGESFAMRHFFGEIESLIPEISMLLSLSHPNITRFLCGFTDEEK 300
           VRRRLG GSQYKEI+WLGE+FA RHFFGEIE L+ EIS+LLSLSHPNI  F CGFTDEEK
Sbjct: 241 VRRRLGAGSQYKEIVWLGETFASRHFFGEIEPLLXEISLLLSLSHPNIVLFPCGFTDEEK 300

Query: 301 KECFLIMELMSRDLSGYVKEICGPRKRIPFTLPVALDLMLQIARGMEYLHSKKVYHGDLN 360
           KECFLIMELMSRDL  Y+K ICGPRKR+PF+LPVA+DLMLQIARGMEYLHSKK+YHGDL+
Sbjct: 301 KECFLIMELMSRDLCSYIKAICGPRKRLPFSLPVAIDLMLQIARGMEYLHSKKIYHGDLD 360

Query: 361 PCNILVKPRAYSTDGYVHGQVSGFGLPAV---KFKNSSNQNESLPFIWYAPEVLEEQDQS 420
           P N+LVK R  ST+GY+  +VSGFGL +V     KN SNQN SL FIWYAPEVLEEQ+Q 
Sbjct: 361 PSNVLVKARGISTEGYLQAKVSGFGLASVNTSNHKNPSNQNGSLSFIWYAPEVLEEQEQG 420

Query: 421 GSAESCKYTEKSDVYSFGMVCFEVLTGKVPFEDSHLQGDKMSRNIRAGERPLFPHSMPKY 480
            S E  KY EKSDVYSFGMVCFE+LTGKVPFEDSHLQGDKMSRNIRAGERPLFP    +Y
Sbjct: 421 KSTEK-KYAEKSDVYSFGMVCFELLTGKVPFEDSHLQGDKMSRNIRAGERPLFPFYXLRY 480

Query: 481 VTNLTKRCWQTDPNQRPSFTSICRILRYTKRFVAMNPDYNSQTDPAMPTVDYCDIESGLL 540
           VTNLTK+CW +DPNQRPSF+SICRILRY KRF+AMNPDYNSQ DP +P VDYC+IESGLL
Sbjct: 481 VTNLTKKCWHSDPNQRPSFSSICRILRYIKRFLAMNPDYNSQLDPPVPMVDYCEIESGLL 540

Query: 541 RRLPSCGISDAASPITDIPFQMFAYRVVEKERAGATFKDTSESGSD-ASACGDETASSID 600
           R++PS G S+  SPI+ IPFQMF YR+ E+ER  ++ KDTSESGSD AS CGDE     D
Sbjct: 541 RKIPSWGSSE-PSPISQIPFQMFVYRIAERERTSSSLKDTSESGSDGASICGDEIVVPAD 600

Query: 601 DPFPTPVERK-LPAPREGSRRLSLTKNNSDVRLNKLPGTPKGRFSRPPQISP-RGRSMRM 660
           DPF T  ERK L +P    ++L   K +SDV+ N+LPGTP+GR SRPPQ++P R  S+R+
Sbjct: 601 DPFSTTPERKSLSSPDSVKKKLPFLKKSSDVKSNRLPGTPRGR-SRPPQMTPCRSVSLRL 660

Query: 661 NSESQLMAISPKIRRL-SGHASDSEL 678
            SE QLMA+SP+IRR  SGHASDSEL
Sbjct: 661 GSEXQLMAMSPRIRRTKSGHASDSEL 682