Cucsa.257860 (gene) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.257860
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	Erythronate-4-phosphate dehydrogenase
Location	scaffold02229 : 5050633 .. 5052419 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AGAAACTTGTGCAGTTCTAGTCCCTCCCTCTCAGCTTATTTTTACACGCAAATAGAAATAAGAATGGCAGTCAAAATCCAATCCGACGAGCTTCATCAAGTTTTGGTTCTAAGTCCCCCGTCGGTATTCACGTCTCTGGAATCACAATTCCAAAaTCGATTCCAGTTTCTCAAACCGTGGGATTCCAATCTCCCACTACTTCAATTCCTCATCTCCAATGCACAATCCGTCCGAGCTTGTCTTGTCACTCCAGGTGACGGACTCGCAGTGTCCTCTGCCATTCTCGACTGCCTCCCCTCCCTCAAGTTTGTCGTCACTGCCAGCGCCGGTGTCGACCACCTGAACGTGGCGGAGCTGCGGCGGCGTGGTGTAGCAATTGCCTATGCAGGGAACTTATTCTCTCAAGATGTTGCTGACATGGCCGTGGGATTACTAATCGACGTCCTTAGGAATGTTTCAGCGGGAGATCGGTTCGTGAGGCAAGGACTTTGGGCTACGCAAATGGATTTTTCTCTCGGATTAAAGGTCCACCTCTGGTTTTGATCTAATGCAAATATTTCGTCAAGGTTTCTAACCAGTTAGGGCAAGTTTGGAGAGTGATCAAATCTCTTGTTCAAAATCATATCAAAACACGGATTTATTCACTAACAATCAATTTTGATGGTAGATTAGTATCTCTTTTAATCATTTTGAGTGGTTAAATTCATCTTTTGAAGTGATTTTGAATATCAGCTGAGCACATCCTCGAACATGCCCTTAATCTATCTTGGAAGAGGCGTAGTTGAATTAGGATTTTTTTtCATCTTTTCTGAATTTCAATGTTGAAAAATTTTCACAGTTGACCGGCAAGCGGATTGGGATTGTTGGATTGGGGAAAATAGGCTCTGAAGTTGCCAAAAGACTGGAGGGTTTTGGGTGCAGAATCTCATATAACTCAAGGACCAAAAAGCCATTAGTTCCATATTCCCACTATTCCAATGTACATGAACTTGCAACCAACTGTGATGTCCTTATCATCTGTTCTAGCTTAACAGAAGAAACTCGCCATTTGATCAACAGAGAGGTGATGGTTGCATTGGGAAAAGATGGAGTAATAATCAATGTCGGTCGAGGGGCAATCATTGATGAGAAGGCGATGATTGAATATTTGATTCAAGGGGAGATTAAGGGGGCTGGGCTGGATGTTTTTGAGGATGAACCTGAAATTCCCAAACAGTTGTTTAATCTTGATAATGTTGTGTTGTCGCCGCATGTTGCTGTCACAACAACTGAATCTATAGCGGGATTGATTGAATTGGCATTGGAGAATTTGGAGGCATTCTTCTCAAACAAACCTTTGGTGTCTCCCTTTCTGGATTAGTTGAGGTGCAGCTGTTTAATGGGGAGGGAGAGAATTTTATGAAAGAGCTCAGAATTTTTGGGTAATTTTAGCTCATTATTATTCAAGGGTGGTGATGTATTTACCACAGTAGTACAATTAAATGGTAAAACGAAAAAAGTAGACAGGCTCTCAACTGATCTAGAAACAGAAAAGCTCCCCAAAGAAGAGCTGCTATTAGTGACAGGAAAGCTATTCCAAAGCAGGTTTCTAGCTTCTAAAATCAATCCTTTCCAGCAAGAGCTTTTCTTCCAAAGCACTTTGCAGGATTTAATGGGATTGATTAAAGGTCCTTTGGAAATTGAATAAGACCCACTTATAGATTATGCATGCATGTAGGTAAAGTCACCAATCACTTGTCTTGAACGGACCTATGTTTGGATAAGTGACCAACTTTCTCTTAAATGGA

mRNA sequence

AGAAACTTGTGCAGTTCTAGTCCCTCCCTCTCAGCTTATTTTTACACGCAAATAGAAATAAGAATGGCAGTCAAAATCCAATCCGACGAGCTTCATCAAGTTTTGGTTCTAAGTCCCCCGTCGGTATTCACGTCTCTGGAATCACAATTCCAAAATCGATTCCAGTTTCTCAAACCGTGGGATTCCAATCTCCCACTACTTCAATTCCTCATCTCCAATGCACAATCCGTCCGAGCTTGTCTTGTCACTCCAGGTGACGGACTCGCAGTGTCCTCTGCCATTCTCGACTGCCTCCCCTCCCTCAAGTTTGTCGTCACTGCCAGCGCCGGTGTCGACCACCTGAACGTGGCGGAGCTGCGGCGGCGTGGTGTAGCAATTGCCTATGCAGGGAACTTATTCTCTCAAGATGTTGCTGACATGGCCGTGGGATTACTAATCGACGTCCTTAGGAATGTTTCAGCGGGAGATCGGTTCGTGAGGCAAGGACTTTGGGCTACGCAAATGGATTTTTCTCTCGGATTAAAGTTGACCGGCAAGCGGATTGGGATTGTTGGATTGGGGAAAATAGGCTCTGAAGTTGCCAAAAGACTGGAGGGTTTTGGGTGCAGAATCTCATATAACTCAAGGACCAAAAAGCCATTAGTTCCATATTCCCACTATTCCAATGTACATGAACTTGCAACCAACTGTGATGTCCTTATCATCTGTTCTAGCTTAACAGAAGAAACTCGCCATTTGATCAACAGAGAGGTGATGGTTGCATTGGGAAAAGATGGAGTAATAATCAATGTCGGTCGAGGGGCAATCATTGATGAGAAGGCGATGATTGAATATTTGATTCAAGGGGAGATTAAGGGGGCTGGGCTGGATGTTTTTGAGGATGAACCTGAAATTCCCAAACAGTTGTTTAATCTTGATAATGTTGTGTTGTCGCCGCATGTTGCTGTCACAACAACTGAATCTATAGCGGGATTGATTGAATTGGCATTGGAGAATTTGGAGGCATTCTTCTCAAACAAACCTTTGGTGTCTCCCTTTCTGGATTAGTTGAGGTGCAGCTGTTTAATGGGGAGGGAGAGAATTTTATGAAAGAGCTCAGAATTTTTGGGTAATTTTAGCTCATTATTATTCAAGGGTGGTGATGTATTTACCACAGTAGTACAATTAAATGGTAAAACGAAAAAAGTAGACAGGCTCTCAACTGATCTAGAAACAGAAAAGCTCCCCAAAGAAGAGCTGCTATTAGTGACAGGAAAGCTATTCCAAAGCAGGTTTCTAGCTTCTAAAATCAATCCTTTCCAGCAAGAGCTTTTCTTCCAAAGCACTTTGCAGGATTTAATGGGATTGATTAAAGGTCCTTTGGAAATTGAATAAGACCCACTTATAGATTATGCATGCATGTAGGTAAAGTCACCAATCACTTGTCTTGAACGGACCTATGTTTGGATAAGTGACCAACTTTCTCTTAAATGGA

Coding sequence (CDS)

ATGGCAGTCAAAATCCAATCCGACGAGCTTCATCAAGTTTTGGTTCTAAGTCCCCCGTCGGTATTCACGTCTCTGGAATCACAATTCCAAAaTCGATTCCAGTTTCTCAAACCGTGGGATTCCAATCTCCCACTACTTCAATTCCTCATCTCCAATGCACAATCCGTCCGAGCTTGTCTTGTCACTCCAGGTGACGGACTCGCAGTGTCCTCTGCCATTCTCGACTGCCTCCCCTCCCTCAAGTTTGTCGTCACTGCCAGCGCCGGTGTCGACCACCTGAACGTGGCGGAGCTGCGGCGGCGTGGTGTAGCAATTGCCTATGCAGGGAACTTATTCTCTCAAGATGTTGCTGACATGGCCGTGGGATTACTAATCGACGTCCTTAGGAATGTTTCAGCGGGAGATCGGTTCGTGAGGCAAGGACTTTGGGCTACGCAAATGGATTTTTCTCTCGGATTAAAGTTGACCGGCAAGCGGATTGGGATTGTTGGATTGGGGAAAATAGGCTCTGAAGTTGCCAAAAGACTGGAGGGTTTTGGGTGCAGAATCTCATATAACTCAAGGACCAAAAAGCCATTAGTTCCATATTCCCACTATTCCAATGTACATGAACTTGCAACCAACTGTGATGTCCTTATCATCTGTTCTAGCTTAACAGAAGAAACTCGCCATTTGATCAACAGAGAGGTGATGGTTGCATTGGGAAAAGATGGAGTAATAATCAATGTCGGTCGAGGGGCAATCATTGATGAGAAGGCGATGATTGAATATTTGATTCAAGGGGAGATTAAGGGGGCTGGGCTGGATGTTTTTGAGGATGAACCTGAAATTCCCAAACAGTTGTTTAATCTTGATAATGTTGTGTTGTCGCCGCATGTTGCTGTCACAACAACTGAATCTATAGCGGGATTGATTGAATTGGCATTGGAGAATTTGGAGGCATTCTTCTCAAACAAACCTTTGGTGTCTCCCTTTCTGGATTAG

Protein sequence

MAVKIQSDELHQVLVLSPPSVFTSLESQFQNRFQFLKPWDSNLPLLQFLISNAQSVRACLVTPGDGLAVSSAILDCLPSLKFVVTASAGVDHLNVAELRRRGVAIAYAGNLFSQDVADMAVGLLIDVLRNVSAGDRFVRQGLWATQMDFSLGLKLTGKRIGIVGLGKIGSEVAKRLEGFGCRISYNSRTKKPLVPYSHYSNVHELATNCDVLIICSSLTEETRHLINREVMVALGKDGVIINVGRGAIIDEKAMIEYLIQGEIKGAGLDVFEDEPEIPKQLFNLDNVVLSPHVAVTTTESIAGLIELALENLEAFFSNKPLVSPFLD*

Homology

[Close]

BLAST of Cucsa.257860 vs. Swiss-Prot
Match: HPR3_ARATH (Glyoxylate/hydroxypyruvate reductase HPR3 OS=Arabidopsis thaliana GN=HPR3 PE=2 SV=1)

HSP 1 Score: 326.6 bits (836), Expect = 3.0e-88
Identity = 174/320 (54.37%), Postives = 226/320 (70.62%), Query Frame = 1


Query: 6   QSDELHQVLVLSPPSVFTSLESQFQNRFQFL-KPWDSNLPLLQFLISNAQSVRACLVTPG 65
           +S E   VL+  PPS+ T ++      F+ L     S+  L  F   +A S RA +++  
Sbjct: 3   ESSEPPVVLLHRPPSL-TFMDEILTREFRTLITDTSSSESLPSFFPRHASSARAFVIS-- 62

Query: 66  DGLAVSSAILDCLPSLKFVVTASAGVDHLNVAELRRRGVAIAYAGNLFSQDVADMAVGLL 125
             L V+  +L  LPSL+ +V  S G+DH+++A  +RRG+ I  AGN FS DVAD AVGLL
Sbjct: 63  GRLPVTDELLSHLPSLQILVCTSVGIDHIDLAACKRRGIVITNAGNAFSDDVADCAVGLL 122

Query: 126 IDVLRNVSAGDRFVRQGLWATQMDFSLGLKLTGKRIGIVGLGKIGSEVAKRLEGFGCRIS 185
           I VLR + A DR+VR G WA   DF LG K++GKR+GIVGLG IGS VAKRLE FGC IS
Sbjct: 123 ISVLRRIPAADRYVRSGNWAKFGDFQLGSKVSGKRVGIVGLGSIGSFVAKRLESFGCVIS 182

Query: 186 YNSRTKKPLVPYSHYSNVHELATNCDVLIICSSLTEETRHLINREVMVALGKDGVIINVG 245
           YNSR++K   PY +YS++  LA N DVL++C SLT+ET H++NREVM  LGKDGV+INVG
Sbjct: 183 YNSRSQKQSSPYRYYSDILSLAENNDVLVLCCSLTDETHHIVNREVMELLGKDGVVINVG 242

Query: 246 RGAIIDEKAMIEYLIQGEIKGAGLDVFEDEPEIPKQLFNLDNVVLSPHVAVTTTESIAGL 305
           RG +IDEK M++ L+ G I GAGLDVFE+EP +P++LF LDNVVLSPH AV T  S+  +
Sbjct: 243 RGKLIDEKEMVKCLVDGVIGGAGLDVFENEPAVPQELFGLDNVVLSPHFAVATPGSLDNV 302

Query: 306 IELALENLEAFFSNKPLVSP 325
            ++AL NL+AFFSN+PL+SP
Sbjct: 303 AQIALANLKAFFSNRPLLSP 319

[Close]

BLAST of Cucsa.257860 vs. Swiss-Prot
Match: HPPR_PLESU (Hydroxyphenylpyruvate reductase OS=Plectranthus scutellarioides GN=HPPR PE=1 SV=2)

HSP 1 Score: 263.5 bits (672), Expect = 3.2e-69
Identity = 137/312 (43.91%), Postives = 201/312 (64.42%), Query Frame = 1


Query: 13  VLVLSPPSVFTSLESQFQNRFQFLKPWDSNLPLLQFLISNAQSVRACLVTPGDGLAVSSA 72
           VL++ P S +  LE +   RF+  + W        FL   A+S+RA  V         + 
Sbjct: 6   VLMMCPMSTY--LEQELDKRFKLFRYWTQPAQR-DFLALQAESIRA--VVGNSNAGADAE 65

Query: 73  ILDCLPSLKFVVTASAGVDHLNVAELRRRGVAIAYAGNLFSQDVADMAVGLLIDVLRNVS 132
           ++D LP L+ V + S G+D +++ +   +GV +    ++ + DVAD+A+GL++ VLR + 
Sbjct: 66  LIDALPKLEIVSSFSVGLDKVDLIKCEEKGVRVTNTPDVLTDDVADLAIGLILAVLRRIC 125

Query: 133 AGDRFVRQGLWATQMDFSLGLKLTGKRIGIVGLGKIGSEVAKRLEGFGCRISYNSRTKKP 192
             D++VR+G W    DF L  K +GKR+GI+GLG+IG  VA+R E F C ISY SR+KKP
Sbjct: 126 ECDKYVRRGAWKFG-DFKLTTKFSGKRVGIIGLGRIGLAVAERAEAFDCPISYFSRSKKP 185

Query: 193 LVPYSHYSNVHELATNCDVLIICSSLTEETRHLINREVMVALGKDGVIINVGRGAIIDEK 252
              Y++Y +V ELA+N D+L++   LT ET H+INREV+ ALG  GV+IN+GRG  +DE 
Sbjct: 186 NTNYTYYGSVVELASNSDILVVACPLTPETTHIINREVIDALGPKGVLINIGRGPHVDEP 245

Query: 253 AMIEYLIQGEIKGAGLDVFEDEPEIPKQLFNLDNVVLSPHVAVTTTESIAGLIELALENL 312
            ++  L++G + GAGLDVFE EPE+P++LF L+NVVL PHV   T E+   + +L + NL
Sbjct: 246 ELVSALVEGRLGGAGLDVFEREPEVPEKLFGLENVVLLPHVGSGTVETRKVMADLVVGNL 305

Query: 313 EAFFSNKPLVSP 325
           EA FS KPL++P
Sbjct: 306 EAHFSGKPLLTP 311

[Close]

BLAST of Cucsa.257860 vs. Swiss-Prot
Match: HPR2_ARATH (Glyoxylate/hydroxypyruvate reductase A HPR2 OS=Arabidopsis thaliana GN=HPR2 PE=1 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 9.5e-66
Identity = 130/312 (41.67%), Postives = 204/312 (65.38%), Query Frame = 1


Query: 13  VLVLSPPSVFTSLESQFQNRFQFLKPWDSNLPLLQFLISNAQSVRACLVTPGDGLAVSSA 72
           VL++ P S +  LE++ + RF  L+ W S    +  L ++  S+RA +     G    + 
Sbjct: 6   VLMMCPMSSY--LENELEKRFNLLRFWTSPEKSV-LLETHRNSIRAVVGNASAG--ADAQ 65

Query: 73  ILDCLPSLKFVVTASAGVDHLNVAELRRRGVAIAYAGNLFSQDVADMAVGLLIDVLRNVS 132
           ++  LP+L+ V + S G+D +++ + + +G+ +    ++ ++DVAD+A+GL++ +LR + 
Sbjct: 66  LISDLPNLEIVSSFSVGLDKIDLGKCKEKGIRVTNTPDVLTEDVADLAIGLILALLRRLC 125

Query: 133 AGDRFVRQGLWATQMDFSLGLKLTGKRIGIVGLGKIGSEVAKRLEGFGCRISYNSRTKKP 192
             DR+VR G W  Q +F L  K +GK +GI+GLG+IG+ +AKR E F C I+Y SRT KP
Sbjct: 126 ECDRYVRSGKWK-QGEFQLTTKFSGKSVGIIGLGRIGTAIAKRAEAFSCPINYYSRTIKP 185

Query: 193 LVPYSHYSNVHELATNCDVLIICSSLTEETRHLINREVMVALGKDGVIINVGRGAIIDEK 252
            V Y +Y  V +LA N D+L++   LTE+TRH+++R+VM ALG  GV+IN+GRG  +DE+
Sbjct: 186 DVAYKYYPTVVDLAQNSDILVVACPLTEQTRHIVDRQVMDALGAKGVLINIGRGPHVDEQ 245

Query: 253 AMIEYLIQGEIKGAGLDVFEDEPEIPKQLFNLDNVVLSPHVAVTTTESIAGLIELALENL 312
            +I+ L +G + GA LDVFE EP +P++LF L+NVVL PHV   T E+   + +L + NL
Sbjct: 246 ELIKALTEGRLGGAALDVFEQEPHVPEELFGLENVVLLPHVGSGTVETRNAMADLVVGNL 305

Query: 313 EAFFSNKPLVSP 325
           EA FS K L++P
Sbjct: 306 EAHFSGKSLLTP 311

[Close]

BLAST of Cucsa.257860 vs. Swiss-Prot
Match: 2KGR_GLUOX (2-ketogluconate reductase OS=Gluconobacter oxydans (strain 621H) GN=GOX0417 PE=1 SV=1)

HSP 1 Score: 202.6 bits (514), Expect = 6.6e-51
Identity = 117/316 (37.03%), Postives = 179/316 (56.65%), Query Frame = 1


Query: 13  VLVLSP--PSVFTSLESQFQNRFQFLKPWDSNLPLLQFLISNAQSVRACLVTPGDGLAVS 72
           +L + P  P +   LE  F      L P+ S    L+ L + A ++R   +T G G  V 
Sbjct: 7   ILTIDPLVPVMKERLEKSFT-----LHPYTS----LENLKNIAPAIRG--ITTGGGSGVP 66

Query: 73  SAILDCLPSLKFVVTASAGVDHLNVAELRRRGVAIAYAGNLFSQDVADMAVGLLIDVLRN 132
           S I+D LP+L+ +     G D +N+ E RRR + +A   N  + DVADMAV L++ V+R+
Sbjct: 67  SEIMDALPNLEVISVNGVGTDRINLDEARRRNIGVAITQNTLTDDVADMAVALMMAVMRS 126

Query: 133 VSAGDRFVRQGLWATQMDFSLGLKLTGKRIGIVGLGKIGSEVAKRLEGFGCRISYNSRTK 192
           +   D FVR G W +     LG  LT K++GI G G IG  +AKR+  FG  ++Y +   
Sbjct: 127 IVTNDAFVRAGKWPSATA-PLGRSLTRKKVGIAGFGHIGQAIAKRVSAFGMEVAYFNSHA 186

Query: 193 KPLVPYSHYSNVHELATNCDVLIICSSLTEETRHLINREVMVALGKDGVIINVGRGAIID 252
           +P        ++  LAT CDVLI+  S    + ++I+R+ + ALGKDG ++N+ RG ++D
Sbjct: 187 RPESTCHFEPDLKALATWCDVLILAVSGGPRSANMIDRDTLDALGKDGFLVNIARGTVVD 246

Query: 253 EKAMIEYLIQGEIKGAGLDVFEDEPEIPKQLFNLDNVVLSPHVAVTTTESIAGLIELALE 312
           E A++  L +  I GAGLDVF++EP I     +L N VL  H A  T E+   +  L ++
Sbjct: 247 EAALLSALQEKRIAGAGLDVFQNEPNINPAFLSLPNTVLQAHQASATVETRTTMANLVVD 306

Query: 313 NLEAFFSNKPLVSPFL 327
           NL A+F++K L++P +
Sbjct: 307 NLIAYFTDKTLLTPVI 310

[Close]

BLAST of Cucsa.257860 vs. Swiss-Prot
Match: GYAR_PYRHO (Glyoxylate reductase OS=Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) GN=gyaR PE=1 SV=2)

HSP 1 Score: 183.3 bits (464), Expect = 4.2e-45
Identity = 97/255 (38.04%), Postives = 149/255 (58.43%), Query Frame = 1


Query: 69  VSSAILDCLPSLKFVVTASAGVDHLNVAELRRRGVAIAYAGNLFSQDVADMAVGLLIDVL 128
           +   + +  P L+ V   + G D++++ E  +RG+ +    ++ +   AD+A  LL+   
Sbjct: 57  IDKEVFENAPKLRIVANYAVGYDNIDIEEATKRGIYVTNTPDVLTDATADLAFALLLATA 116

Query: 129 RNVSAGDRFVRQGLW-----ATQMDFSLGLKLTGKRIGIVGLGKIGSEVAKRLEGFGCRI 188
           R+V  GDRFVR G W     A    + LG  + GK IGI+GLG+IG  +AKR +GF  RI
Sbjct: 117 RHVVKGDRFVRSGEWKKRGVAWHPKWFLGYDVYGKTIGIIGLGRIGQAIAKRAKGFNMRI 176

Query: 189 SYNSRTKKPLVPY---SHYSNVHELATNCDVLIICSSLTEETRHLINREVMVALGKDGVI 248
            Y SRT+K  V     + +  + +L    D +++   LT ET HLIN E +  + K  ++
Sbjct: 177 LYYSRTRKEEVERELNAEFKPLEDLLRESDFVVLAVPLTRETYHLINEERLKLMKKTAIL 236

Query: 249 INVGRGAIIDEKAMIEYLIQGEIKGAGLDVFEDEPEIPKQLFNLDNVVLSPHVAVTTTES 308
           IN+ RG ++D  A+++ L +G I GAGLDVFE+EP   ++LF LDNVVL+PH+   +  +
Sbjct: 237 INIARGKVVDTNALVKALKEGWIAGAGLDVFEEEPYYNEELFKLDNVVLTPHIGSASFGA 296

Query: 309 IAGLIELALENLEAF 316
             G+ EL  +NL AF
Sbjct: 297 REGMAELVAKNLIAF 311

[Close]

BLAST of Cucsa.257860 vs. TrEMBL
Match: A0A0A0L4C2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G124920 PE=3 SV=1)

HSP 1 Score: 637.9 bits (1644), Expect = 6.9e-180
Identity = 327/327 (100.00%), Postives = 327/327 (100.00%), Query Frame = 1


Query: 1   MAVKIQSDELHQVLVLSPPSVFTSLESQFQNRFQFLKPWDSNLPLLQFLISNAQSVRACL 60
           MAVKIQSDELHQVLVLSPPSVFTSLESQFQNRFQFLKPWDSNLPLLQFLISNAQSVRACL
Sbjct: 1   MAVKIQSDELHQVLVLSPPSVFTSLESQFQNRFQFLKPWDSNLPLLQFLISNAQSVRACL 60

Query: 61  VTPGDGLAVSSAILDCLPSLKFVVTASAGVDHLNVAELRRRGVAIAYAGNLFSQDVADMA 120
           VTPGDGLAVSSAILDCLPSLKFVVTASAGVDHLNVAELRRRGVAIAYAGNLFSQDVADMA
Sbjct: 61  VTPGDGLAVSSAILDCLPSLKFVVTASAGVDHLNVAELRRRGVAIAYAGNLFSQDVADMA 120

Query: 121 VGLLIDVLRNVSAGDRFVRQGLWATQMDFSLGLKLTGKRIGIVGLGKIGSEVAKRLEGFG 180
           VGLLIDVLRNVSAGDRFVRQGLWATQMDFSLGLKLTGKRIGIVGLGKIGSEVAKRLEGFG
Sbjct: 121 VGLLIDVLRNVSAGDRFVRQGLWATQMDFSLGLKLTGKRIGIVGLGKIGSEVAKRLEGFG 180

Query: 181 CRISYNSRTKKPLVPYSHYSNVHELATNCDVLIICSSLTEETRHLINREVMVALGKDGVI 240
           CRISYNSRTKKPLVPYSHYSNVHELATNCDVLIICSSLTEETRHLINREVMVALGKDGVI
Sbjct: 181 CRISYNSRTKKPLVPYSHYSNVHELATNCDVLIICSSLTEETRHLINREVMVALGKDGVI 240

Query: 241 INVGRGAIIDEKAMIEYLIQGEIKGAGLDVFEDEPEIPKQLFNLDNVVLSPHVAVTTTES 300
           INVGRGAIIDEKAMIEYLIQGEIKGAGLDVFEDEPEIPKQLFNLDNVVLSPHVAVTTTES
Sbjct: 241 INVGRGAIIDEKAMIEYLIQGEIKGAGLDVFEDEPEIPKQLFNLDNVVLSPHVAVTTTES 300

Query: 301 IAGLIELALENLEAFFSNKPLVSPFLD 328
           IAGLIELALENLEAFFSNKPLVSPFLD
Sbjct: 301 IAGLIELALENLEAFFSNKPLVSPFLD 327

[Close]

BLAST of Cucsa.257860 vs. TrEMBL
Match: A0A0A0L908_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G124900 PE=3 SV=1)

HSP 1 Score: 467.6 bits (1202), Expect = 1.2e-128
Identity = 242/326 (74.23%), Postives = 275/326 (84.36%), Query Frame = 1


Query: 1   MAVKIQSDELHQVLVLSPPSVFTSLESQFQNRFQFLKPWDSNLPLLQFLISNAQSVRACL 60
           MA++ +S EL +VLVL PP VF++LESQF N+F +LKPW S LPL QFL S AQS +A L
Sbjct: 1   MAMEGESKELPEVLVLGPPWVFSTLESQFPNKFHYLKPWLSQLPLHQFLTSYAQSTQALL 60

Query: 61  VTPGDGLAVSSAILDCLPSLKFVVTASAGVDHLNVAELRRRGVAIAYAGNLFSQDVADMA 120
           +     L  +S ILDCLPSLK VVT SAGV+HLN AELR RG+A+AYAGN+FS+DVADMA
Sbjct: 61  IPVSPPL--NSPILDCLPSLKLVVTVSAGVEHLNFAELRGRGIAVAYAGNVFSEDVADMA 120

Query: 121 VGLLIDVLRNVSAGDRFVRQGLWATQMDFSLGLKLTGKRIGIVGLGKIGSEVAKRLEGFG 180
           VGLLIDVLR VSAGDRFV+Q L   + DF L  KL+GK+IGIVGLGKIGSEVAKRLEGFG
Sbjct: 121 VGLLIDVLRKVSAGDRFVKQRLQPIKPDFPLRSKLSGKQIGIVGLGKIGSEVAKRLEGFG 180

Query: 181 CRISYNSRTKKPLVPYSHYSNVHELATNCDVLIICSSLTEETRHLINREVMVALGKDGVI 240
           CRISYNSRTKKPLV YS+YSNVHELATNC+VLIIC  LTEET H+INREVM+ LGKDGVI
Sbjct: 181 CRISYNSRTKKPLVSYSYYSNVHELATNCEVLIICCGLTEETHHMINREVMLELGKDGVI 240

Query: 241 INVGRGAIIDEKAMIEYLIQGEIKGAGLDVFEDEPEIPKQLFNLDNVVLSPHVAVTTTES 300
           IN+GRGA+IDEK MI  LI+GEI GAGLDVFE EPEIPKQLF LDNVVLSPH AVTT ES
Sbjct: 241 INIGRGAVIDEKEMIRCLIEGEIGGAGLDVFEFEPEIPKQLFTLDNVVLSPHTAVTTHES 300

Query: 301 IAGLIELALENLEAFFSNKPLVSPFL 327
             G+ +LA+ENLEAFFSNKPL+SP++
Sbjct: 301 FVGIAKLAVENLEAFFSNKPLLSPYV 324

[Close]

BLAST of Cucsa.257860 vs. TrEMBL
Match: A0A0A0L7K6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G124910 PE=3 SV=1)

HSP 1 Score: 453.8 bits (1166), Expect = 1.8e-124
Identity = 233/332 (70.18%), Postives = 268/332 (80.72%), Query Frame = 1


Query: 1   MAVKIQSDELHQVLVLSPPSVFTSLESQFQNRFQFLKPWDSNLPLLQFLISNAQSVRACL 60
           MA + Q+ EL Q+L+L PPS+F  LESQF NRF FLKPW  NLPL QFL S AQS +A L
Sbjct: 1   MAAEEQAKELPQILILGPPSIFPYLESQFSNRFLFLKPWLYNLPLTQFLTSYAQSTQALL 60

Query: 61  VTPGDGLAVSSAILDCLPSLKFVVTASAGVDHLNVAELRRRGVAIAYAGNLFSQDVADMA 120
           +  G    ++S I+DCLPSLK VVT+S GVDHL+  ELRRRGVAIA AGNLFS+D ADMA
Sbjct: 61  IRGGGNTQLTSTIIDCLPSLKLVVTSSVGVDHLDFPELRRRGVAIANAGNLFSEDTADMA 120

Query: 121 -----VGLLIDVLRNVSAGDRFVRQGLWATQMDFSLGLKLTGKRIGIVGLGKIGSEVAKR 180
                VGLLIDVLR +SAGDRFVRQGLW+ + DF  GLKL+GKRIGIVGLGKIGSEVAKR
Sbjct: 121 GGGVAVGLLIDVLRKISAGDRFVRQGLWSKKEDFPPGLKLSGKRIGIVGLGKIGSEVAKR 180

Query: 181 LEGFGCRISYNSRTKKPLVPYSHYSNVHELATNCDVLIICSSLTEETRHLINREVMVALG 240
           LEGFGC+ISYNSRTKK + PYS+Y NV+ELA N + LIIC +LT+ET HLIN+EVM+ALG
Sbjct: 181 LEGFGCKISYNSRTKKSMAPYSYYPNVYELAANTEALIICCALTKETYHLINKEVMLALG 240

Query: 241 KDGVIINVGRGAIIDEKAMIEYLIQGEIKGAGLDVFEDEPEIPKQLFNLDNVVLSPHVAV 300
           KDGVI+N+GRG IIDEK MI  L QGEI GAGLDVFE+EP +P++LFNLDNVVLSPH AV
Sbjct: 241 KDGVIVNIGRGLIIDEKEMIRCLTQGEIGGAGLDVFENEPNVPEELFNLDNVVLSPHAAV 300

Query: 301 TTTESIAGLIELALENLEAFFSNKPLVSPFLD 328
            T ES   L +L + NLEAFFSNKPLVSP +D
Sbjct: 301 MTYESKVELSKLVVNNLEAFFSNKPLVSPVVD 332

[Close]

BLAST of Cucsa.257860 vs. TrEMBL
Match: M5XK89_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa025117mg PE=3 SV=1)

HSP 1 Score: 399.8 bits (1026), Expect = 3.2e-108
Identity = 202/327 (61.77%), Postives = 251/327 (76.76%), Query Frame = 1


Query: 1   MAVKIQSDELHQVLVLSPPSVFTSLESQFQNRFQFLKPWDSNLPLLQFLISNAQSVRACL 60
           MA+     EL QVLVL PP  FT LES + ++F+FLK WDS LP  QFL ++A SV+A L
Sbjct: 1   MALNGNHSELPQVLVLLPPGCFTLLESNYSHKFKFLKAWDSPLPQDQFLATHAGSVQA-L 60

Query: 61  VTPGDGLAVSSAILDCLPSLKFVVTASAGVDHLNVAELRRRGVAIAYAGNLFSQDVADMA 120
           ++  +G  +++ IL  LPSLK +VT S G+DHL++AE R RGVAIA    +F++DVADMA
Sbjct: 61  LSSANGPTITANILQMLPSLKVIVTTSVGIDHLDLAECRSRGVAIASTPKIFTEDVADMA 120

Query: 121 VGLLIDVLRNVSAGDRFVRQGLWATQMDFSLGLKLTGKRIGIVGLGKIGSEVAKRLEGFG 180
           VGLL+DV+R +SAGDR+VR GLWAT+ D++LG K+ GKR+GIVGLG IG EVAKRLE FG
Sbjct: 121 VGLLLDVMRKISAGDRYVRDGLWATRGDYALGSKIGGKRVGIVGLGNIGLEVAKRLEAFG 180

Query: 181 CRISYNSRTKKPLVPYSHYSNVHELATNCDVLIICSSLTEETRHLINREVMVALGKDGVI 240
           C I YNSRT+KP V Y  YS++ ELA N D L+IC +LT ET HLIN++V++ALG+DGVI
Sbjct: 181 CNILYNSRTEKPFVSYPFYSDICELAANSDALVICCALTAETHHLINKKVLLALGRDGVI 240

Query: 241 INVGRGAIIDEKAMIEYLIQGEIKGAGLDVFEDEPEIPKQLFNLDNVVLSPHVAVTTTES 300
           +N+GRGAIIDEK M+  L+ GEI GAGLDVFEDEPE+P++LF LDNVVLSPH A  T E 
Sbjct: 241 VNIGRGAIIDEKEMVRCLVIGEIGGAGLDVFEDEPEVPEELFALDNVVLSPHYATYTPEC 300

Query: 301 IAGLIELALENLEAFFSNKPLVSPFLD 328
              L EL   N EAFFSNKPL+SP +D
Sbjct: 301 FMALCELVAGNFEAFFSNKPLLSPAVD 326

[Close]

BLAST of Cucsa.257860 vs. TrEMBL
Match: M5XDE9_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa008547mg PE=3 SV=1)

HSP 1 Score: 394.8 bits (1013), Expect = 1.0e-106
Identity = 201/327 (61.47%), Postives = 248/327 (75.84%), Query Frame = 1


Query: 1   MAVKIQSDELHQVLVLSPPSVFTSLESQFQNRFQFLKPWDSNLPLLQFLISNAQSVRACL 60
           MA+     EL QVLVL PP  FT LES + ++F+FLK WDS LP  QFL ++A SV+A L
Sbjct: 1   MALNGNHSELPQVLVLLPPGCFTLLESNYSHKFKFLKAWDSPLPQDQFLATHAGSVQA-L 60

Query: 61  VTPGDGLAVSSAILDCLPSLKFVVTASAGVDHLNVAELRRRGVAIAYAGNLFSQDVADMA 120
           ++   G  +++ IL  LPSLK +VT  AG+DHL++AE R RGVAIA    +F++DVADMA
Sbjct: 61  LSSVKGPTITANILQMLPSLKVIVTTGAGIDHLDLAECRSRGVAIASTPKIFTEDVADMA 120

Query: 121 VGLLIDVLRNVSAGDRFVRQGLWATQMDFSLGLKLTGKRIGIVGLGKIGSEVAKRLEGFG 180
           VGLL+DV+R +SAGDR+VR GLWAT+ D++LG K+ G R+GIVGLG IG EVAKRLE FG
Sbjct: 121 VGLLLDVMRKISAGDRYVRDGLWATRGDYALGSKIGGNRVGIVGLGNIGVEVAKRLEAFG 180

Query: 181 CRISYNSRTKKPLVPYSHYSNVHELATNCDVLIICSSLTEETRHLINREVMVALGKDGVI 240
           C I YNSRT+KP V Y  YS++ ELA N D L+IC +LT ET HLIN++V + LG+DGVI
Sbjct: 181 CNILYNSRTEKPFVSYPFYSDICELAANSDALVICCALTAETHHLINKKVSLKLGRDGVI 240

Query: 241 INVGRGAIIDEKAMIEYLIQGEIKGAGLDVFEDEPEIPKQLFNLDNVVLSPHVAVTTTES 300
           +NVGRGAIIDEK M+  L++GEI GAGLDVFEDEPE+P++LF LDNVVLSPH A  T E 
Sbjct: 241 VNVGRGAIIDEKEMVRCLVKGEIGGAGLDVFEDEPEVPEELFALDNVVLSPHYATYTPEC 300

Query: 301 IAGLIELALENLEAFFSNKPLVSPFLD 328
              L EL   N EAFFSNKPL+SP +D
Sbjct: 301 FMALCELVAGNFEAFFSNKPLLSPAVD 326

[Close]

BLAST of Cucsa.257860 vs. TAIR10
Match: AT2G45630.2 (AT2G45630.2 D-isomer specific 2-hydroxyacid dehydrogenase family protein)

HSP 1 Score: 329.3 bits (843), Expect = 2.6e-90
Identity = 172/329 (52.28%), Postives = 232/329 (70.52%), Query Frame = 1


Query: 1   MAVKIQSDELHQVLVLSPPSVFTSLESQF--QNRFQFLKPWDSNLPLLQFLISNAQSVRA 60
           MA    +++L +VL++  P     L   F    +F+ LK ++S LPL +FL  ++ S+ A
Sbjct: 12  MATSSSTEKLPRVLIVKRPDAMAVLGDGFVASTKFEILKAFESPLPLPEFLAYHSDSISA 71

Query: 61  CLVTPGDGLAVSSAILDCLPSLKFVVTASAGVDHLNVAELRRRGVAIAYAGNLFSQDVAD 120
            +        V++ ++  LP+L+ VVT SAGVDH+++ E RRRG+++A AG+ FS+DVAD
Sbjct: 72  IIAPVA--APVTADLIRILPNLRLVVTTSAGVDHVDLVECRRRGISVANAGSSFSEDVAD 131

Query: 121 MAVGLLIDVLRNVSAGDRFVRQGLWATQMDFSLGLKLTGKRIGIVGLGKIGSEVAKRLEG 180
            AVGLLIDV R +SA +RFV+Q  W  + D+ LG KL  KRIGIVGLG IGS+VA RL+ 
Sbjct: 132 TAVGLLIDVFRRISAANRFVKQRFWPLKGDYPLGSKLGRKRIGIVGLGSIGSKVATRLDA 191

Query: 181 FGCRISYNSRTKKPL-VPYSHYSNVHELATNCDVLIICSSLTEETRHLINREVMVALGKD 240
           FGC+ISY+SR +KP  VPY +Y ++ E+A N D LIIC  L E+T  LIN++V+ ALGK 
Sbjct: 192 FGCQISYSSRNRKPYDVPYHYYMDIEEMAANSDALIICCELNEKTLRLINKDVLSALGKR 251

Query: 241 GVIINVGRGAIIDEKAMIEYLIQGEIKGAGLDVFEDEPEIPKQLFNLDNVVLSPHVAVTT 300
           GVI+NV RGAIIDE+ M+  L +GEI GAGLDVFEDEP +PK+LF LDNVV SPH A  T
Sbjct: 252 GVIVNVARGAIIDEEEMVRCLREGEIGGAGLDVFEDEPNVPKELFELDNVVFSPHSAFMT 311

Query: 301 TESIAGLIELALENLEAFFSNKPLVSPFL 327
            E +  L ++ + N+EAFFSNKPL++P L
Sbjct: 312 LEGLEELGKVVVGNIEAFFSNKPLLTPVL 338

[Close]

BLAST of Cucsa.257860 vs. TAIR10
Match: AT1G12550.1 (AT1G12550.1 D-isomer specific 2-hydroxyacid dehydrogenase family protein)

HSP 1 Score: 326.6 bits (836), Expect = 1.7e-89
Identity = 174/320 (54.37%), Postives = 226/320 (70.62%), Query Frame = 1


Query: 6   QSDELHQVLVLSPPSVFTSLESQFQNRFQFL-KPWDSNLPLLQFLISNAQSVRACLVTPG 65
           +S E   VL+  PPS+ T ++      F+ L     S+  L  F   +A S RA +++  
Sbjct: 3   ESSEPPVVLLHRPPSL-TFMDEILTREFRTLITDTSSSESLPSFFPRHASSARAFVIS-- 62

Query: 66  DGLAVSSAILDCLPSLKFVVTASAGVDHLNVAELRRRGVAIAYAGNLFSQDVADMAVGLL 125
             L V+  +L  LPSL+ +V  S G+DH+++A  +RRG+ I  AGN FS DVAD AVGLL
Sbjct: 63  GRLPVTDELLSHLPSLQILVCTSVGIDHIDLAACKRRGIVITNAGNAFSDDVADCAVGLL 122

Query: 126 IDVLRNVSAGDRFVRQGLWATQMDFSLGLKLTGKRIGIVGLGKIGSEVAKRLEGFGCRIS 185
           I VLR + A DR+VR G WA   DF LG K++GKR+GIVGLG IGS VAKRLE FGC IS
Sbjct: 123 ISVLRRIPAADRYVRSGNWAKFGDFQLGSKVSGKRVGIVGLGSIGSFVAKRLESFGCVIS 182

Query: 186 YNSRTKKPLVPYSHYSNVHELATNCDVLIICSSLTEETRHLINREVMVALGKDGVIINVG 245
           YNSR++K   PY +YS++  LA N DVL++C SLT+ET H++NREVM  LGKDGV+INVG
Sbjct: 183 YNSRSQKQSSPYRYYSDILSLAENNDVLVLCCSLTDETHHIVNREVMELLGKDGVVINVG 242

Query: 246 RGAIIDEKAMIEYLIQGEIKGAGLDVFEDEPEIPKQLFNLDNVVLSPHVAVTTTESIAGL 305
           RG +IDEK M++ L+ G I GAGLDVFE+EP +P++LF LDNVVLSPH AV T  S+  +
Sbjct: 243 RGKLIDEKEMVKCLVDGVIGGAGLDVFENEPAVPQELFGLDNVVLSPHFAVATPGSLDNV 302

Query: 306 IELALENLEAFFSNKPLVSP 325
            ++AL NL+AFFSN+PL+SP
Sbjct: 303 AQIALANLKAFFSNRPLLSP 319

[Close]

BLAST of Cucsa.257860 vs. TAIR10
Match: AT1G79870.1 (AT1G79870.1 D-isomer specific 2-hydroxyacid dehydrogenase family protein)

HSP 1 Score: 251.9 bits (642), Expect = 5.4e-67
Identity = 130/312 (41.67%), Postives = 204/312 (65.38%), Query Frame = 1


Query: 13  VLVLSPPSVFTSLESQFQNRFQFLKPWDSNLPLLQFLISNAQSVRACLVTPGDGLAVSSA 72
           VL++ P S +  LE++ + RF  L+ W S    +  L ++  S+RA +     G    + 
Sbjct: 6   VLMMCPMSSY--LENELEKRFNLLRFWTSPEKSV-LLETHRNSIRAVVGNASAG--ADAQ 65

Query: 73  ILDCLPSLKFVVTASAGVDHLNVAELRRRGVAIAYAGNLFSQDVADMAVGLLIDVLRNVS 132
           ++  LP+L+ V + S G+D +++ + + +G+ +    ++ ++DVAD+A+GL++ +LR + 
Sbjct: 66  LISDLPNLEIVSSFSVGLDKIDLGKCKEKGIRVTNTPDVLTEDVADLAIGLILALLRRLC 125

Query: 133 AGDRFVRQGLWATQMDFSLGLKLTGKRIGIVGLGKIGSEVAKRLEGFGCRISYNSRTKKP 192
             DR+VR G W  Q +F L  K +GK +GI+GLG+IG+ +AKR E F C I+Y SRT KP
Sbjct: 126 ECDRYVRSGKWK-QGEFQLTTKFSGKSVGIIGLGRIGTAIAKRAEAFSCPINYYSRTIKP 185

Query: 193 LVPYSHYSNVHELATNCDVLIICSSLTEETRHLINREVMVALGKDGVIINVGRGAIIDEK 252
            V Y +Y  V +LA N D+L++   LTE+TRH+++R+VM ALG  GV+IN+GRG  +DE+
Sbjct: 186 DVAYKYYPTVVDLAQNSDILVVACPLTEQTRHIVDRQVMDALGAKGVLINIGRGPHVDEQ 245

Query: 253 AMIEYLIQGEIKGAGLDVFEDEPEIPKQLFNLDNVVLSPHVAVTTTESIAGLIELALENL 312
            +I+ L +G + GA LDVFE EP +P++LF L+NVVL PHV   T E+   + +L + NL
Sbjct: 246 ELIKALTEGRLGGAALDVFEQEPHVPEELFGLENVVLLPHVGSGTVETRNAMADLVVGNL 305

Query: 313 EAFFSNKPLVSP 325
           EA FS K L++P
Sbjct: 306 EAHFSGKSLLTP 311

[Close]

BLAST of Cucsa.257860 vs. TAIR10
Match: AT5G28310.1 (AT5G28310.1 NAD(P)-binding Rossmann-fold superfamily protein)

HSP 1 Score: 117.1 bits (292), Expect = 2.1e-26
Identity = 77/194 (39.69%), Postives = 99/194 (51.03%), Query Frame = 1


Query: 135 DRFVRQGLWATQMDF-SLGLKLTGKRIGIVGLGKIGSEVAKRLEGFGCRISYNSRTKKPL 194
           D FV Q    +  D   L    + KRIGIVGLG IGS+VA RL+ FGC+ISY+SR +KP 
Sbjct: 91  DYFVNQSASTSGYDDPDLNQYQSKKRIGIVGLGSIGSKVATRLKAFGCQISYSSRNRKPY 150

Query: 195 -VPYSHYSNVHELATNCDVLIICSSLTEETRHLINREVMVALGKDGVIINVGRGAIIDEK 254
            VPY +Y ++ E+                                GVI+NV  GAIIDE+
Sbjct: 151 AVPYHYYMDIEEM-------------------------------HGVIVNVALGAIIDEE 210

Query: 255 AMIEYLIQGEIKGAGLDVFEDEPEIPKQLFNLDNVVLSPHVAVTTTESIAGLIELALENL 314
            M                      +PK+LF LDNVV SPH A  T E +  L ++ + N+
Sbjct: 211 EM--------------------SNVPKELFELDNVVFSPHCAFMTLEGLEELGKVVVGNI 233

Query: 315 EAFFSNKPLVSPFL 327
           EAFFSNKPL++P L
Sbjct: 271 EAFFSNKPLLTPVL 233

[Close]

BLAST of Cucsa.257860 vs. TAIR10
Match: AT1G68010.2 (AT1G68010.2 hydroxypyruvate reductase)

HSP 1 Score: 110.9 bits (276), Expect = 1.5e-24
Identity = 71/269 (26.39%), Postives = 136/269 (50.56%), Query Frame = 1


Query: 87  SAGVDHLNVAELRRRGVAIAYAGNLFSQDVADMAVGLLIDVLRNVSAGDRFVRQGLWATQ 146
           + G ++++V    + G+A+     + ++  A++A  L +   R +   D F+R GL+   
Sbjct: 94  AVGYNNVDVEAANKYGIAVGNTPGVLTETTAELAASLSLAAARRIVEADEFMRGGLYEGW 153

Query: 147 MD-FSLGLKLTGKRIGIVGLGKIGSEVAKRL-EGFGCRISY-----NSRTKKPLVPYSHY 206
           +    +G  L G+ +G++G G+IGS  A+ + EGF   + Y     ++R +K +  Y  +
Sbjct: 154 LPHLFVGNLLKGQTVGVIGAGRIGSAYARMMVEGFKMNLIYFDLYQSTRLEKFVTAYGQF 213

Query: 207 --------------SNVHELATNCDVLIICSSLTEETRHLINREVMVALGK-DGVIINVG 266
                         S++ E+    D++ +   L + T HL+N+E +  + K + +++N  
Sbjct: 214 LKANGEQPVTWKRASSMEEVLREADLISLHPVLDKTTYHLVNKERLAMMKKVEAILVNCS 273

Query: 267 RGAIIDEKAMIEYLIQGEIKGAGLDVFEDEPEIPKQLFNLDNVVLSPHVAVTTTESIAGL 326
           RG +IDE A++E+L +  +   GLDVFE+EP +   L +  N ++ PH+A  +  +  G+
Sbjct: 274 RGPVIDEAALVEHLKENPMFRVGLDVFEEEPFMKPGLADTKNAIVVPHIASASKWTREGM 333

Query: 327 IELALENLEAFFSNKPL------VSPFLD 328
             LA  N+       P+      V PFL+
Sbjct: 334 ATLAALNVLGRVKGYPIWHDPNRVDPFLN 362

[Close]

BLAST of Cucsa.257860 vs. NCBI nr
Match: gi|778677375|ref|XP_004134341.2| (PREDICTED: glyoxylate/hydroxypyruvate reductase HPR3-like [Cucumis sativus])

HSP 1 Score: 637.9 bits (1644), Expect = 9.9e-180
Identity = 327/327 (100.00%), Postives = 327/327 (100.00%), Query Frame = 1


Query: 1   MAVKIQSDELHQVLVLSPPSVFTSLESQFQNRFQFLKPWDSNLPLLQFLISNAQSVRACL 60
           MAVKIQSDELHQVLVLSPPSVFTSLESQFQNRFQFLKPWDSNLPLLQFLISNAQSVRACL
Sbjct: 1   MAVKIQSDELHQVLVLSPPSVFTSLESQFQNRFQFLKPWDSNLPLLQFLISNAQSVRACL 60

Query: 61  VTPGDGLAVSSAILDCLPSLKFVVTASAGVDHLNVAELRRRGVAIAYAGNLFSQDVADMA 120
           VTPGDGLAVSSAILDCLPSLKFVVTASAGVDHLNVAELRRRGVAIAYAGNLFSQDVADMA
Sbjct: 61  VTPGDGLAVSSAILDCLPSLKFVVTASAGVDHLNVAELRRRGVAIAYAGNLFSQDVADMA 120

Query: 121 VGLLIDVLRNVSAGDRFVRQGLWATQMDFSLGLKLTGKRIGIVGLGKIGSEVAKRLEGFG 180
           VGLLIDVLRNVSAGDRFVRQGLWATQMDFSLGLKLTGKRIGIVGLGKIGSEVAKRLEGFG
Sbjct: 121 VGLLIDVLRNVSAGDRFVRQGLWATQMDFSLGLKLTGKRIGIVGLGKIGSEVAKRLEGFG 180

Query: 181 CRISYNSRTKKPLVPYSHYSNVHELATNCDVLIICSSLTEETRHLINREVMVALGKDGVI 240
           CRISYNSRTKKPLVPYSHYSNVHELATNCDVLIICSSLTEETRHLINREVMVALGKDGVI
Sbjct: 181 CRISYNSRTKKPLVPYSHYSNVHELATNCDVLIICSSLTEETRHLINREVMVALGKDGVI 240

Query: 241 INVGRGAIIDEKAMIEYLIQGEIKGAGLDVFEDEPEIPKQLFNLDNVVLSPHVAVTTTES 300
           INVGRGAIIDEKAMIEYLIQGEIKGAGLDVFEDEPEIPKQLFNLDNVVLSPHVAVTTTES
Sbjct: 241 INVGRGAIIDEKAMIEYLIQGEIKGAGLDVFEDEPEIPKQLFNLDNVVLSPHVAVTTTES 300

Query: 301 IAGLIELALENLEAFFSNKPLVSPFLD 328
           IAGLIELALENLEAFFSNKPLVSPFLD
Sbjct: 301 IAGLIELALENLEAFFSNKPLVSPFLD 327

[Close]

BLAST of Cucsa.257860 vs. NCBI nr
Match: gi|659075398|ref|XP_008438122.1| (PREDICTED: glyoxylate/hydroxypyruvate reductase HPR3-like [Cucumis melo])

HSP 1 Score: 577.0 bits (1486), Expect = 2.1e-161
Identity = 296/327 (90.52%), Postives = 305/327 (93.27%), Query Frame = 1


Query: 1   MAVKIQSDELHQVLVLSPPSVFTSLESQFQNRFQFLKPWDSNLPLLQFLISNAQSVRACL 60
           MAV+I SDELHQVLVLSPP  FTSLESQFQNRFQFL PW+SNLPLLQFLISNAQSVRACL
Sbjct: 1   MAVEIPSDELHQVLVLSPPFEFTSLESQFQNRFQFLNPWNSNLPLLQFLISNAQSVRACL 60

Query: 61  VTPGDGLAVSSAILDCLPSLKFVVTASAGVDHLNVAELRRRGVAIAYAGNLFSQDVADMA 120
           V PG  LAVSSAILDCLPSL+ VVT SAGVDHL+VAE+RRRGVAIA+AGNL SQDVADMA
Sbjct: 61  VMPGGRLAVSSAILDCLPSLELVVTTSAGVDHLHVAEIRRRGVAIAFAGNLLSQDVADMA 120

Query: 121 VGLLIDVLRNVSAGDRFVRQGLWATQMDFSLGLKLTGKRIGIVGLGKIGSEVAKRLEGFG 180
           VGLLIDVLRN+SAGDRFVRQGLW TQ DFS+GLKL+GKRIGIVGLGKIGSEVAKRLEGFG
Sbjct: 121 VGLLIDVLRNISAGDRFVRQGLWPTQGDFSIGLKLSGKRIGIVGLGKIGSEVAKRLEGFG 180

Query: 181 CRISYNSRTKKPLVPYSHYSNVHELATNCDVLIICSSLTEETRHLINREVMVALGKDGVI 240
           CRISYNSRTKKPLVPYSHYSNVHELATNCD LIIC  LTEETRHLINREVMVALGKDGVI
Sbjct: 181 CRISYNSRTKKPLVPYSHYSNVHELATNCDALIICCCLTEETRHLINREVMVALGKDGVI 240

Query: 241 INVGRGAIIDEKAMIEYLIQGEIKGAGLDVFEDEPEIPKQLFNLDNVVLSPHVAVTTTES 300
           INVGRGAIIDEKAMIE LIQGEIKGAGLDVFE EPEIPKQLFNLDNVVLSPH A TTTES
Sbjct: 241 INVGRGAIIDEKAMIECLIQGEIKGAGLDVFEHEPEIPKQLFNLDNVVLSPHAAGTTTES 300

Query: 301 IAGLIELALENLEAFFSNKPLVSPFLD 328
           I GL ELA ENLEAFFSNKPLVSPFLD
Sbjct: 301 IVGLTELAFENLEAFFSNKPLVSPFLD 327

[Close]

BLAST of Cucsa.257860 vs. NCBI nr
Match: gi|449433109|ref|XP_004134340.1| (PREDICTED: glyoxylate/hydroxypyruvate reductase HPR3 [Cucumis sativus])

HSP 1 Score: 467.6 bits (1202), Expect = 1.8e-128
Identity = 242/326 (74.23%), Postives = 275/326 (84.36%), Query Frame = 1


Query: 1   MAVKIQSDELHQVLVLSPPSVFTSLESQFQNRFQFLKPWDSNLPLLQFLISNAQSVRACL 60
           MA++ +S EL +VLVL PP VF++LESQF N+F +LKPW S LPL QFL S AQS +A L
Sbjct: 1   MAMEGESKELPEVLVLGPPWVFSTLESQFPNKFHYLKPWLSQLPLHQFLTSYAQSTQALL 60

Query: 61  VTPGDGLAVSSAILDCLPSLKFVVTASAGVDHLNVAELRRRGVAIAYAGNLFSQDVADMA 120
           +     L  +S ILDCLPSLK VVT SAGV+HLN AELR RG+A+AYAGN+FS+DVADMA
Sbjct: 61  IPVSPPL--NSPILDCLPSLKLVVTVSAGVEHLNFAELRGRGIAVAYAGNVFSEDVADMA 120

Query: 121 VGLLIDVLRNVSAGDRFVRQGLWATQMDFSLGLKLTGKRIGIVGLGKIGSEVAKRLEGFG 180
           VGLLIDVLR VSAGDRFV+Q L   + DF L  KL+GK+IGIVGLGKIGSEVAKRLEGFG
Sbjct: 121 VGLLIDVLRKVSAGDRFVKQRLQPIKPDFPLRSKLSGKQIGIVGLGKIGSEVAKRLEGFG 180

Query: 181 CRISYNSRTKKPLVPYSHYSNVHELATNCDVLIICSSLTEETRHLINREVMVALGKDGVI 240
           CRISYNSRTKKPLV YS+YSNVHELATNC+VLIIC  LTEET H+INREVM+ LGKDGVI
Sbjct: 181 CRISYNSRTKKPLVSYSYYSNVHELATNCEVLIICCGLTEETHHMINREVMLELGKDGVI 240

Query: 241 INVGRGAIIDEKAMIEYLIQGEIKGAGLDVFEDEPEIPKQLFNLDNVVLSPHVAVTTTES 300
           IN+GRGA+IDEK MI  LI+GEI GAGLDVFE EPEIPKQLF LDNVVLSPH AVTT ES
Sbjct: 241 INIGRGAVIDEKEMIRCLIEGEIGGAGLDVFEFEPEIPKQLFTLDNVVLSPHTAVTTHES 300

Query: 301 IAGLIELALENLEAFFSNKPLVSPFL 327
             G+ +LA+ENLEAFFSNKPL+SP++
Sbjct: 301 FVGIAKLAVENLEAFFSNKPLLSPYV 324

[Close]

BLAST of Cucsa.257860 vs. NCBI nr
Match: gi|659077733|ref|XP_008439355.1| (PREDICTED: glyoxylate/hydroxypyruvate reductase HPR3-like [Cucumis melo])

HSP 1 Score: 457.2 bits (1175), Expect = 2.4e-125
Identity = 237/318 (74.53%), Postives = 273/318 (85.85%), Query Frame = 1


Query: 12  QVLVLSPPSVFTSLESQFQNRFQFLKPW-DSNLPLLQFLISNAQSVRACL--VTPGDGLA 71
           +VLVL PP VF++LESQF N+F++LKPW    LPL QFL S AQS +A L  V P     
Sbjct: 8   EVLVLGPPWVFSTLESQFPNKFRYLKPWLFPQLPLHQFLTSYAQSTQALLMPVIP----L 67

Query: 72  VSSAILDCLPSLKFVVTASAGVDHLNVAELRRRGVAIAYAGNLFSQDVADMAVGLLIDVL 131
           ++SA LDCLPSLK VVTASAGV+HLN+A+LR RG+A+AYAGN+FS+DVADMAVGLLIDVL
Sbjct: 68  LNSATLDCLPSLKLVVTASAGVEHLNLADLRGRGIAVAYAGNVFSEDVADMAVGLLIDVL 127

Query: 132 RNVSAGDRFVRQGLWATQMDFSLGLKLTGKRIGIVGLGKIGSEVAKRLEGFGCRISYNSR 191
           R VSAGDRFV+Q L  T++ F LG KL+GK+IGIVGLGKIG+EVA+RLEGFGC ISYNSR
Sbjct: 128 RKVSAGDRFVKQRLQPTKLHFPLGSKLSGKQIGIVGLGKIGTEVARRLEGFGCTISYNSR 187

Query: 192 TKKPLVPYSHYSNVHELATNCDVLIICSSLTEETRHLINREVMVALGKDGVIINVGRGAI 251
           TKKPLV YS+YSNVHELATNC+VLIIC  LTEETRH+INREVM+ALGKDGVIIN+GRGA+
Sbjct: 188 TKKPLVSYSYYSNVHELATNCEVLIICCGLTEETRHMINREVMLALGKDGVIINIGRGAV 247

Query: 252 IDEKAMIEYLIQGEIKGAGLDVFEDEPEIPKQLFNLDNVVLSPHVAVTTTESIAGLIELA 311
           I+EK MI  LI+GEI GAGLDVFE EPEIPKQLF LDNVVLSPH A+TT ES  G+ +LA
Sbjct: 248 IEEKEMIRCLIEGEIGGAGLDVFEFEPEIPKQLFTLDNVVLSPHAAITTHESFGGMAKLA 307

Query: 312 LENLEAFFSNKPLVSPFL 327
           +ENLEAFFSNKPLVSP+L
Sbjct: 308 VENLEAFFSNKPLVSPYL 321

[Close]

BLAST of Cucsa.257860 vs. NCBI nr
Match: gi|778677370|ref|XP_004133897.2| (PREDICTED: glyoxylate/hydroxypyruvate reductase HPR3-like [Cucumis sativus])

HSP 1 Score: 453.8 bits (1166), Expect = 2.6e-124
Identity = 233/332 (70.18%), Postives = 268/332 (80.72%), Query Frame = 1


Query: 1   MAVKIQSDELHQVLVLSPPSVFTSLESQFQNRFQFLKPWDSNLPLLQFLISNAQSVRACL 60
           MA + Q+ EL Q+L+L PPS+F  LESQF NRF FLKPW  NLPL QFL S AQS +A L
Sbjct: 1   MAAEEQAKELPQILILGPPSIFPYLESQFSNRFLFLKPWLYNLPLTQFLTSYAQSTQALL 60

Query: 61  VTPGDGLAVSSAILDCLPSLKFVVTASAGVDHLNVAELRRRGVAIAYAGNLFSQDVADMA 120
           +  G    ++S I+DCLPSLK VVT+S GVDHL+  ELRRRGVAIA AGNLFS+D ADMA
Sbjct: 61  IRGGGNTQLTSTIIDCLPSLKLVVTSSVGVDHLDFPELRRRGVAIANAGNLFSEDTADMA 120

Query: 121 -----VGLLIDVLRNVSAGDRFVRQGLWATQMDFSLGLKLTGKRIGIVGLGKIGSEVAKR 180
                VGLLIDVLR +SAGDRFVRQGLW+ + DF  GLKL+GKRIGIVGLGKIGSEVAKR
Sbjct: 121 GGGVAVGLLIDVLRKISAGDRFVRQGLWSKKEDFPPGLKLSGKRIGIVGLGKIGSEVAKR 180

Query: 181 LEGFGCRISYNSRTKKPLVPYSHYSNVHELATNCDVLIICSSLTEETRHLINREVMVALG 240
           LEGFGC+ISYNSRTKK + PYS+Y NV+ELA N + LIIC +LT+ET HLIN+EVM+ALG
Sbjct: 181 LEGFGCKISYNSRTKKSMAPYSYYPNVYELAANTEALIICCALTKETYHLINKEVMLALG 240

Query: 241 KDGVIINVGRGAIIDEKAMIEYLIQGEIKGAGLDVFEDEPEIPKQLFNLDNVVLSPHVAV 300
           KDGVI+N+GRG IIDEK MI  L QGEI GAGLDVFE+EP +P++LFNLDNVVLSPH AV
Sbjct: 241 KDGVIVNIGRGLIIDEKEMIRCLTQGEIGGAGLDVFENEPNVPEELFNLDNVVLSPHAAV 300

Query: 301 TTTESIAGLIELALENLEAFFSNKPLVSPFLD 328
            T ES   L +L + NLEAFFSNKPLVSP +D
Sbjct: 301 MTYESKVELSKLVVNNLEAFFSNKPLVSPVVD 332

The following BLAST results are available for this feature:

BLAST of Cucsa.257860 vs. Swiss-Prot


Match Name	E-value	Identity	Description
HPR3_ARATH	3.0e-88	54.38	Glyoxylate/hydroxypyruvate reductase HPR3 OS=Arabidopsis thaliana GN=HPR3 PE=2 S...	[more]
HPPR_PLESU	3.2e-69	43.91	Hydroxyphenylpyruvate reductase OS=Plectranthus scutellarioides GN=HPPR PE=1 SV=...	[more]
HPR2_ARATH	9.5e-66	41.67	Glyoxylate/hydroxypyruvate reductase A HPR2 OS=Arabidopsis thaliana GN=HPR2 PE=1...	[more]
2KGR_GLUOX	6.6e-51	37.03	2-ketogluconate reductase OS=Gluconobacter oxydans (strain 621H) GN=GOX0417 PE=1...	[more]
GYAR_PYRHO	4.2e-45	38.04	Glyoxylate reductase OS=Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / ...	[more]

BLAST of Cucsa.257860 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L4C2_CUCSA	6.9e-180	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G124920 PE=3 SV=1	[more]
A0A0A0L908_CUCSA	1.2e-128	74.23	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G124900 PE=3 SV=1	[more]
A0A0A0L7K6_CUCSA	1.8e-124	70.18	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G124910 PE=3 SV=1	[more]
M5XK89_PRUPE	3.2e-108	61.77	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa025117mg PE=3 SV=1	[more]
M5XDE9_PRUPE	1.0e-106	61.47	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa008547mg PE=3 SV=1	[more]

BLAST of Cucsa.257860 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G45630.2	2.6e-90	52.28	D-isomer specific 2-hydroxyacid dehydrogenase family protein	[more]
AT1G12550.1	1.7e-89	54.38	D-isomer specific 2-hydroxyacid dehydrogenase family protein	[more]
AT1G79870.1	5.4e-67	41.67	D-isomer specific 2-hydroxyacid dehydrogenase family protein	[more]
AT5G28310.1	2.1e-26	39.69	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT1G68010.2	1.5e-24	26.39	hydroxypyruvate reductase	[more]

BLAST of Cucsa.257860 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778677375\|ref\|XP_004134341.2\|	9.9e-180	100.00	PREDICTED: glyoxylate/hydroxypyruvate reductase HPR3-like [Cucumis sativus]	[more]
gi\|659075398\|ref\|XP_008438122.1\|	2.1e-161	90.52	PREDICTED: glyoxylate/hydroxypyruvate reductase HPR3-like [Cucumis melo]	[more]
gi\|449433109\|ref\|XP_004134340.1\|	1.8e-128	74.23	PREDICTED: glyoxylate/hydroxypyruvate reductase HPR3 [Cucumis sativus]	[more]
gi\|659077733\|ref\|XP_008439355.1\|	2.4e-125	74.53	PREDICTED: glyoxylate/hydroxypyruvate reductase HPR3-like [Cucumis melo]	[more]
gi\|778677370\|ref\|XP_004133897.2\|	2.6e-124	70.18	PREDICTED: glyoxylate/hydroxypyruvate reductase HPR3-like [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR006139	D-isomer_2_OHA_DH_cat_dom
IPR006140	D-isomer_DH_NAD-bd
IPR016040	NAD(P)-bd_dom

Vocabulary: **Molecular Function**
Term	Definition
GO:0016616	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
GO:0051287	NAD binding

Vocabulary: **Biological Process**
Term	Definition
GO:0008152	metabolic process
GO:0055114	oxidation-reduction process

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0008152	metabolic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0051287	NAD binding
molecular_function	GO:0016616	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsa.257860.1	Cucsa.257860.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006139	D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain	PFAM	PF00389	2-Hacid_dh	coord: 49..321 score: 8.0
IPR006140	D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding domain	PFAM	PF02826	2-Hacid_dh_C	coord: 121..294 score: 4.3
IPR016040	NAD(P)-binding domain	GENE3D	G3DSA:3.40.50.720		coord: 13..127 score: 1.5E-20coord: 128..297 score: 7.1
IPR016040	NAD(P)-binding domain	unknown	SSF51735	NAD(P)-binding Rossmann-fold domains	coord: 113..294 score: 6.26
None	No IPR available	PANTHER	PTHR10996	2-HYDROXYACID DEHYDROGENASE-RELATED	coord: 1..327 score: 3.1E
None	No IPR available	PANTHER	PTHR10996:SF155	D-ISOMER SPECIFIC 2-HYDROXYACID DEHYDROGENASE-LIKE PROTEIN-RELATED	coord: 1..327 score: 3.1E
None	No IPR available	unknown	SSF52283	Formate/glycerate dehydrogenase catalytic domain-like	coord: 14..148 score: 8.5

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cucsa.257860	Cla021283	Watermelon (97103) v1	cgywmB479
Cucsa.257860	Csa3G124900	Cucumber (Chinese Long) v2	cgycuB366
Cucsa.257860	MELO3C006164	Melon (DHL92) v3.5.1	cgymeB439
Cucsa.257860	ClCG05G002010	Watermelon (Charleston Gray)	cgywcgB451
Cucsa.257860	CSPI03G09310	Wild cucumber (PI 183967)	cgycpiB383
Cucsa.257860	CmaCh14G019960	Cucurbita maxima (Rimu)	cgycmaB0709
Cucsa.257860	CmaCh06G011200	Cucurbita maxima (Rimu)	cgycmaB0727
Cucsa.257860	CmoCh06G011480	Cucurbita moschata (Rifu)	cgycmoB0718
Cucsa.257860	CmoCh14G020560	Cucurbita moschata (Rifu)	cgycmoB0700
Cucsa.257860	Lsi05G019850	Bottle gourd (USVL1VR-Ls)	cgylsiB415
Cucsa.257860	Cp4.1LG08g01840	Cucurbita pepo (Zucchini)	cgycpeB0687
Cucsa.257860	Cp4.1LG03g17030	Cucurbita pepo (Zucchini)	cgycpeB0682
Cucsa.257860	MELO3C006164.2	Melon (DHL92) v3.6.1	cgymedB432
Cucsa.257860	CsaV3_3G009430	Cucumber (Chinese Long) v3	cgycucB387
Cucsa.257860	Cla97C05G082460	Watermelon (97103) v2	cgywmbB452
Cucsa.257860	Bhi01G000210	Wax gourd	cgywgoB515

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cucsa.257860	Silver-seed gourd	carcgyB0559
Cucsa.257860	Silver-seed gourd	carcgyB0693
Cucsa.257860	Cucumber (Chinese Long) v3	cgycucB394
Cucsa.257860	Cucumber (Chinese Long) v3	cgycucB398
Cucsa.257860	Watermelon (97103) v2	cgywmbB446
Cucsa.257860	Watermelon (97103) v2	cgywmbB455
Cucsa.257860	Cucumber (Gy14) v1	cgycgyB125
Cucsa.257860	Cucumber (Gy14) v1	cgycgyB130
Cucsa.257860	Cucurbita maxima (Rimu)	cgycmaB0733
Cucsa.257860	Cucurbita moschata (Rifu)	cgycmoB0722
Cucsa.257860	Wild cucumber (PI 183967)	cgycpiB389
Cucsa.257860	Wild cucumber (PI 183967)	cgycpiB393
Cucsa.257860	Cucumber (Chinese Long) v2	cgycuB374
Cucsa.257860	Melon (DHL92) v3.5.1	cgymeB427
Cucsa.257860	Melon (DHL92) v3.5.1	cgymeB429
Cucsa.257860	Watermelon (Charleston Gray)	cgywcgB454
Cucsa.257860	Watermelon (97103) v1	cgywmB482
Cucsa.257860	Watermelon (97103) v1	cgywmB484
Cucsa.257860	Cucurbita pepo (Zucchini)	cgycpeB0670
Cucsa.257860	Cucurbita pepo (Zucchini)	cgycpeB0677
Cucsa.257860	Bottle gourd (USVL1VR-Ls)	cgylsiB410
Cucsa.257860	Melon (DHL92) v3.6.1	cgymedB421
Cucsa.257860	Melon (DHL92) v3.6.1	cgymedB423