Cucsa.257530 (gene) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.257530
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	tRNA N6-adenosine threonylcarbamoyltransferase
Location	scaffold02229 : 4790600 .. 4795228 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GTCAGCGGGACGAGGTTCGAGTTTGTGTTGAAACGCGTTGGTTAATATCTGTTCAGAAAGTTAGACTAAATATACAACTTACAAAAATGTAATAACCAACTTCTTTGGTTTTCAGTTTTTGAAAATCAAGCCTGTAACTACCCCTTGTGTTTGTTATCTATTCATTACCAATGTTTCCGGAAAAAAAAaaaGAAGTAATTTTTAAGAACTTTTGTTGCTTTTAGAATTTGGTTAAGAATTCAACTTTTTGGTCTAAATTTATAATGCCACATCAAGGCAGAGCTGCTTGCTCGCTATGGAGGCGTTGCTCCTAAAATGGCAGAAGAAGCGCACGCACAAGTGATTGACAAGGTTATGATTGCAAGGTATACTAAATACTCCAATTTCAGCATCCTTTTCATCCACTCTTACATTGGCTTCGGTTATTTTTCGTCTGTCAACTTCAGGTGGTGCAGGAAACAATGGAAAAGGCTAACTTAACAGAAAGGGATTTATCTGCAGTTGCTGTGACCATTGGCCCTGGTTTGAGCCTCTGCCTCCGTGGTAATTTTGCAGGATCCCTCCTTATGGTTTAAGTCCTAAGTTGTACTCTGTCTACTATGTGGACATCTTTCCGCTAATTGAAAGCCGTTGTTTTATCTCGTTATCAGTCACTGTTCAACAGACACCTAAGCTGTAGATTTCTGTAAAAAGAAAGGTTTAGATTTATGTTTTAATGCTTATGTGTACAGGAAAATTGTTCTTAGGCTTTTAGTTATAGAAAGACGCACTGTTCTCTACACTACGTATAAGTTCACAATCAATTTGGCTTCTTTTTCTATCTTTAATTATTCAAGTGAAAATTTATTTTTAATTGGAGATCCTTCGGACCCATTCACCATGCAATTGAAATGCAGTTGGAGTGCAGAAGGCACGAAAAATTGCTGGTAGCTCTAGTCTACCAATTGTTGGGGTGCATCACATGGAAGCTCATGCTCTGGTAGCTAGGTATGTATAACAAATTAAAAATGCAAGTTACATCTGGGTCAGCTTATATTTGTTAATCTGTGAAAGATTTTTCAAAGGAAACTATTTGATGGTGTTTACTAGAGGATGAATATTTGATTTCGTTTGTACTTTCTGGTTGGACACTCTACTCTTCTTATTAAAACATTATTCTTCATTCTGTAGATTAGTTGAACGGGAACTGCAATTTCCTTTCATGGCACTGCTCATTTCTGGTAAAAATTTGTATTCTTATTTTCCTTCCCCTGCATTCTTCCTTCATCTTCATTACATAGATACAAGTTGTGTTTACGTTTCCTGTGTCTAGATGTGTCAAAAATAGAAAGGATACAGAGTGATTTTTTAAACTACTTGATTGTTTCCAACTTTTAGTTTCAAAATTTTAGAGACTTTTATAATTTTATACATCTCCAAATCCAATTATGAAATGCTTGCAAGAGGTTATCTTTTCATAGTTGTTAAGATTAGTTCTCTTTTTTGATAGCATTAAATTTAAATGGTCGAGCTCTGTCATTACCATCTCAGCACATTATTTGAACTATTTTCACAATTCTTTAGCAACAGAGCCTACGGTTCTGATTGTTGATTTTTCTATCTGGGTTGAACTTCAACTTTTCATGAATAGAACTTGTGCTGTAATTTTCAATGATTCATGGATGGGACGCTTGAAAAAGACGACAAACTCATACTTTATTTTTAATCAGCCACGATGGAGAGAAAAAGGAAAACTGGATCTTTTTCATGGCTTTAGGGTTAAACTTTGTCCGTGTCATCATATCAGTTTATTCTATTAGCTATTCATTGAGAAGTTAATTATTGGGTCCTCTTGAACGTGTTATTAGGATGACTCAAAATGGTTTCGGTTGCTTGCATTCGAGCTTTTGAGTTTTGACAAAGATTTTAAAGAATGAAAAATTAAATGTCATACATTCTAGGGGGACACAATTTACTTGTTCTCGCACAAGATCTTGGCCAATACATACAACTTGGAACAACAATAGACGATGCCATTGGGGAGGCGTATGACAAGACTGCCAAATGGCTTGGTCTTGATCTGAGGAAGAGTGGTGGGCCGGCTATAGAGGAGCTTGCCAGAGAGGGGGATCCAAAGTCAATAAAGTTTTCTGTAAGTCTTCAAACTCATTATGTTGTTCGTCATTGCATATGCCTGTTTTTATCGTGTAGGTGGTGCATAAACATGAGAAACATTTTATAACCATACATTTCTATGTTAGAGTGTACATTAAGTTCTTCAAATATTTCTTTCTTTAGACTCCAATGAAGCAACATAAAGATTGCAACTTCTCCTATGCGGGGCTGAAGACCCAAGTCAGGCTGGCTATTGAATCCCGAAACATGTGTGTATTTATCTGGAATGTGCATACTTACAATTTTTATAAAGGAAACTGATTTTCTAAATATTTTTTtGTTATCTTATTATCTTATTCATCCTTTCTAATAACAAAATCACCAGCAAGTATAATATACAGAAAAATGAAAATCCATATATGTAAAAAAGGGTCAGGCATAGTTAATATTTATTGTATTGCAAGATTAAAGGTCTATTAGATTTGAAGTTTTGATATCTCTATCGGAATTTAAATAACCCATGGTTTTAATTTCACATGTAATATCTGAGCAATTGGGTTTTCTGTCTTGCGTAGTTTGTTTCCATATCTACAGTTTCGTCTGATTGCTAGGAACTGGGTTGATGGTTATGTCTGAGAAGCTTATTGAAGAGACTTTtGATGGGTTGAAAGTTGCTGTTTTAATTTATTTTCTGAGTCATTTGATGAATGTACATGCCGTGTGCAGTGACACAAAAATTCCTCTGTCGTCTGCAAGCATCCAAGACAGAAGTGTAAGGGCTGATATAGCTGCCTCCTTTCAGGTATCCATCAGTCTGTAACTGTATGGACTAAATTTATATTGTTACATCAACTGATCCATTTGAAATTCATTGCAGCGTGTTGCAGTGCTACATCTAGAGGACAAATGTGAACGAGCAATTGAATGGGCGTTGAAGATTGAACCATCCATAAGACATTTGGTAAAATGAGACGAAAAACTTCTTTTGTTCCTTTATATCTCATGATTCTTTCATCTTACATTTACATTTTCTTGCCAGTGAGCTGCACACATAGTTGAAGTGAGGAATTCTCTTATGTTCTGCCTATGCAGGTTGTCTCAGGAGGTGTTGCATCAAATAAGTATGTTAGGGCTCAACTTATGGCGGTTGTCAGGAAGAAGGGCCTGCAGCTTGTCTGCCCTCCTCCCAGTCTCTGCACTGATAATGGTATAATTTCTTTAGCTCAACTTGATGTTAAATTGTGGATATGTTTGTGTCTACTAAATTAATAAACAGTTCAGAACCTGAACCTGAGACATCAAACTGTAGGTTGACCAATTATCATTTGAAAATAATTGATGCTCGTGATTTCTTTTTGAGCTCCAAGTGTGCTAGTTGATGTGTTTGTGAGAGAATTATAGAGCTTTCCTAAATAAAGTAGCGATTTCTTATGCAGAAGAATCTTAATATACAACGGCTTTGCTGATCATTCCTGGTGCAAGCTTATTTAAATAAATTCATACACTATATGGTATTCATATTTGTTTCACGCCATAGGAACCCATTTACTTTTAATTGTGTTGGCAAAGGTGTAATGGTGGCTTGGACGGGCCTCGAGCATTTTCGCATAGGGAGATTTGATCCTCCTCCACCAGCTGATGAACCTGAAGATACAATGGTATGGTTCTAATCCCTCATCTGTAATCCCTCTGCCCCTTGAATTAAATAGAAACAAGTTAGTTGATGAAGCTATCAAATGTTTGGGTTCTGCCTCTGCTTTTGAGCGTATAAAAAGAATAATACAAGCCCATGGGGTGTCAGGCGAGCTGGTCTCGAGTTAGAACGTTTGCGTCAGTTTGACGTCAGCCCCTTCTGAAGTTATGGATACAATTATGTTGTCCAAAATGATTTGAAGAGCCTGTAGAATATTCTAGTTTATGATGACAGTATCCTCCTTTTATTTTCTGGTTTTTCGATCCTTCTCAAGTTTACATTTTGTTCCCAGTATGACGTGCGACCGAGATGGCCATTAGGAGAAGAATACACCGAGGGAAGGAGTGCAGCTCGCTCACTAAGAACAGCCCGAATCCATCCATCACTTACGTCTATCATCCAAGCATCTCTACAGCAACAACAGTAGCTGTAGCTTCAGTTCCCAAATTCCAACAAAaGGAATTTGCATTCTTTTGCATCTCTTCTTCCATGTGCGACTTCTTAGACAACAAATGGAATCATATCAGGAGCTCGATGAAGCACCCGGGCCATCAATGCGGAACTTCCAATGCTCAATGCCTTCTGTTAACTTATTGGGAGATTTTGGTTGGTTTCATGAGATATGCCATCGATATGTTGCATTATGTAGTGATTCACCTGTATCATACTTACAACTAAGAAGACTCTTATATTTGTTACAGTTTGTATCATATTCTCATTTATAAAAGAAAAACAGAGACCTTTTTTCACATTtTTTTTTtCTTTtAAAATTTGGATCATTTGTCACCTTAGATCTTTGAAGTGGATGTTTTAGACAGTTATTTTGTAACAAATATTGACAT

mRNA sequence

GTCAGCGGGACGAGGTTCGAGTTTGTGTTGAAACGCGTTGGTTAATATCTGTTCAGAAAGTTAGACTAAATATACAACTTACaaaaatAGCTGCTTGCTCGCTATGGAGGCGTTGCTCCTAAAATGGCAGAAGAAGCGCACGCACAAGTGATTGACAAGGTTATGATTGCAAGGTATACTAAATACTCCAATTTCAGCATCCTTTTCATCCACTCTTACATTGGCTTCGGTTATTTTTCGTCTGTCAACTTCAGGTGGTGCAGGAAACAATGGAAAAGGCTAACTTAACAGAAAGGGATTTATCTGCAGTTGCTGTGACCATTGGCCCTGGTTTGAGCCTCTGCCTCCGTGTTGGAGTGCAGAAGGCACGAAAAATTGCTGGTAGCTCTAGTCTACCAATTGTTGGGGTGCATCACATGGAAGCTCATGCTCTGGTAGCTAGATTAGTTGAACGGGAACTGCAATTTCCTTTCATGGCACTGCTCATTTCTGGGGGACACAATTTACTTGTTCTCGCACAAGATCTTGGCCAATACATACAACTTGGAACAACAATAGACGATGCCATTGGGGAGGCGTATGACAAGACTGCCAAATGGCTTGGTCTTGATCTGAGGAAGAGTGGTGGGCCGGCTATAGAGGAGCTTGCCAGAGAGGGGGATCCAAAGTCAATAAAGTTTTCTACTCCAATGAAGCAACATAAAGATTGCAACTTCTCCTATGCGGGGCTGAAGACCCAAGTCAGGCTGGCTATTGAATCCCGAAACATTGACACAAAAATTCCTCTGTCGTCTGCAAGCATCCAAGACAGAAGTGTAAGGGCTGATATAGCTGCCTCCTTTCAGCGTGTTGCAGTGCTACATCTAGAGGACAAATGTGAACGAGCAATTGAATGGGCGTTGAAGATTGAACCATCCATAAGACATTTGGTTGTCTCAGGAGGTGTTGCATCAAATAAGTATGTTAGGGCTCAACTTATGGCGGTTGTCAGGAAGAAGGGCCTGCAGCTTGTCTGCCCTCCTCCCAGTCTCTGCACTGATAATGGTGTAATGGTGGCTTGGACGGGCCTCGAGCATTTTCGCATAGGGAGATTTGATCCTCCTCCACCAGCTGATGAACCTGAAGATACAATGTATGACGTGCGACCGAGATGGCCATTAGGAGAAGAATACACCGAGGGAAGGAGTGCAGCTCGCTCACTAAGAACAGCCCGAATCCATCCATCACTTACGTCTATCATCCAAGCATCTCTACAGCAACAACAGTAGCTGTAGCTTCAGTTCCCAAATTCCAACAAAAGGAATTTGCATTCTTTTGCATCTCTTCTTCCATGTGCGACTTCTTAGACAACAAATGGAATCATATCAGGAGCTCGATGAAGCACCCGGGCCATCAATGCGGAACTTCCAATGCTCAATGCCTTCTGTTAACTTATTGGGAGATTTTGGTTGGTTTCATGAGATATGCCATCGATATGTTGCATTATGTAGTGATTCACCTGTATCATACTTACAACTAAGAAGACTCTTATATTTGTTACAGTTTGTATCATATTCTCATTTATAAAAGAAAAACAGAGACCTTTTTTCACATTTTTTTTTTCTTTTAAAATTTGGATCATTTGTCACCTTAGATCTTTGAAGTGGATGTTTTAGACAGTTATTTTGTAACAAATATTGACAT

Coding sequence (CDS)

ATGGAAAAGGCTAACTTAACAGAAAGGGATTTATCTGCAGTTGCTGTGACCATTGGCCCTGGTTTGAGCCTCTGCCTCCGTGTTGGAGTGCAGAAGGCACGAAAAATTGCTGGTAGCTCTAGTCTACCAATTGTTGGGGTGCATCACATGGAAGCTCATGCTCTGGTAGCTAGATTAGTTGAACGGGAACTGCAATTTCCTTTCATGGCACTGCTCATTTCTGGGGGACACAATTTACTTGTTCTCGCACAAGATCTTGGCCAATACATACAACTTGGAACAACAATAGACGATGCCATTGGGGAGGCGTATGACAAGACTGCCAAATGGCTTGGTCTTGATCTGAGGAAGAGTGGTGGGCCGGCTATAGAGGAGCTTGCCAGAGAGGGGGATCCAAAGTCAATAAAGTTTTCTACTCCAATGAAGCAACATAAAGATTGCAACTTCTCCTATGCGGGGCTGAAGACCCAAGTCAGGCTGGCTATTGAATCCCGAAACATTGACACAAAAATTCCTCTGTCGTCTGCAAGCATCCAAGACAGAAGTGTAAGGGCTGATATAGCTGCCTCCTTTCAGCGTGTTGCAGTGCTACATCTAGAGGACAAATGTGAACGAGCAATTGAATGGGCGTTGAAGATTGAACCATCCATAAGACATTTGGTTGTCTCAGGAGGTGTTGCATCAAATAAGTATGTTAGGGCTCAACTTATGGCGGTTGTCAGGAAGAAGGGCCTGCAGCTTGTCTGCCCTCCTCCCAGTCTCTGCACTGATAATGGTGTAATGGTGGCTTGGACGGGCCTCGAGCATTTTCGCATAGGGAGATTTGATCCTCCTCCACCAGCTGATGAACCTGAAGATACAATGTATGACGTGCGACCGAGATGGCCATTAGGAGAAGAATACACCGAGGGAAGGAGTGCAGCTCGCTCACTAAGAACAGCCCGAATCCATCCATCACTTACGTCTATCATCCAAGCATCTCTACAGCAACAACAGTAG

Protein sequence

MEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSSSLPIVGVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAYDKTAKWLGLDLRKSGGPAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLAIESRNIDTKIPLSSASIQDRSVRADIAASFQRVAVLHLEDKCERAIEWALKIEPSIRHLVVSGGVASNKYVRAQLMAVVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHFRIGRFDPPPPADEPEDTMYDVRPRWPLGEEYTEGRSAARSLRTARIHPSLTSIIQASLQQQQ*

Homology

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BLAST of Cucsa.257530 vs. Swiss-Prot
Match: OSGP2_ARATH (Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial OS=Arabidopsis thaliana GN=GCP1 PE=2 SV=2)

HSP 1 Score: 553.1 bits (1424), Expect = 2.0e-156
Identity = 266/332 (80.12%), Postives = 303/332 (91.27%), Query Frame = 1


Query: 1   MEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSSSLPIVGVHHMEAHALVARLV 60
           ++KANLTE+DLSAVAVTIGPGLSLCLRVGV+KAR++AG+ SLPIVGVHHMEAHALVARLV
Sbjct: 146 LDKANLTEKDLSAVAVTIGPGLSLCLRVGVRKARRVAGNFSLPIVGVHHMEAHALVARLV 205

Query: 61  ERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAYDKTAKWLGLDLRKSGG 120
           E+EL FPFMALLISGGHNLLVLA  LGQY QLGTT+DDAIGEA+DKTAKWLGLD+ +SGG
Sbjct: 206 EQELSFPFMALLISGGHNLLVLAHKLGQYTQLGTTVDDAIGEAFDKTAKWLGLDMHRSGG 265

Query: 121 PAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLAIESRNIDTKIPLSSASIQD 180
           PA+EELA EGD KS+KF+ PMK HKDCNFSYAGLKTQVRLAIE++ ID K P+SSA+ +D
Sbjct: 266 PAVEELALEGDAKSVKFNVPMKYHKDCNFSYAGLKTQVRLAIEAKEIDAKCPVSSATNED 325

Query: 181 RSVRADIAASFQRVAVLHLEDKCERAIEWALKIEPSIRHLVVSGGVASNKYVRAQLMAVV 240
           R  RADIAASFQRVAVLHLE+KCERAI+WAL++EPSI+H+V+SGGVASNKYVR +L  +V
Sbjct: 326 RRNRADIAASFQRVAVLHLEEKCERAIDWALELEPSIKHMVISGGVASNKYVRLRLNNIV 385

Query: 241 RKKGLQLVCPPPSLCTDNGVMVAWTGLEHFRIGRFDPPPPADEPEDTMYDVRPRWPLGEE 300
             K L+LVCPPPSLCTDNGVMVAWTGLEHFR+GR+DPPPPA EPED +YD+RPRWPLGEE
Sbjct: 386 ENKNLKLVCPPPSLCTDNGVMVAWTGLEHFRVGRYDPPPPATEPEDYVYDLRPRWPLGEE 445

Query: 301 YTEGRSAARSLRTARIHPSLTSIIQASLQQQQ 333
           Y +GRS ARS+RTARIHPSLTSII+A   QQQ
Sbjct: 446 YAKGRSEARSMRTARIHPSLTSIIRADSLQQQ 477

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BLAST of Cucsa.257530 vs. Swiss-Prot
Match: TSAD_GLUOX (tRNA N6-adenosine threonylcarbamoyltransferase OS=Gluconobacter oxydans (strain 621H) GN=tsaD PE=3 SV=2)

HSP 1 Score: 218.4 bits (555), Expect = 1.2e-55
Identity = 130/301 (43.19%), Postives = 171/301 (56.81%), Query Frame = 1


Query: 1   MEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSSSLPIVGVHHMEAHALVARL- 60
           ++KA+LT  D+   A T GPGL   L VG   A+ +A +   P V V+H+EAH L  RL 
Sbjct: 74  LKKASLTLADIDTFAGTTGPGLIGGLIVGSSYAKGLAMALHRPFVAVNHIEAHILTPRLP 133

Query: 61  -VERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAYDKTAKWLGLDLRKS 120
            +  +L FP++ +L+SGGH   V  ++ G+Y++LG TIDDA GEA+DK AK LGL     
Sbjct: 134 SLGADLHFPYLTMLVSGGHCQCVSVEETGRYVRLGGTIDDAAGEAFDKVAKMLGLSW--P 193

Query: 121 GGPAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLAIESRNIDTKIPLSSASI 180
           GGPA+E+LA EG   +     P+K  + C+FS++GLKT V     SR IDT+ P  S   
Sbjct: 194 GGPALEKLATEGRDDAYPLPRPLKGREGCDFSFSGLKTAV-----SRLIDTQDPTGSRDA 253

Query: 181 QDRSVRADIAASFQRVAVLHLEDKCERAIEWALKIEPSIRHLVVSGGVASNKYVRAQLMA 240
             R   AD+AASFQR     + D+ E     AL + P+   LVV+GGVA+NK +R  L  
Sbjct: 254 LPRQFAADVAASFQRAVADVMADRAEH----ALALSPNATALVVAGGVAANKTLRHALEQ 313

Query: 241 VVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHFRIGRFDPPPPADEPEDTMYDVRPRWPLG 300
           V    G+    PP  LCTDN VMVAW  LE    G        + P +     RPRWPL 
Sbjct: 314 VAANHGIPFFAPPLRLCTDNAVMVAWAALERLHAG--------ETPNEIDTAARPRWPLS 355

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BLAST of Cucsa.257530 vs. Swiss-Prot
Match: OSGP2_XENTR (Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial OS=Xenopus tropicalis GN=osgepl1 PE=2 SV=2)

HSP 1 Score: 218.0 bits (554), Expect = 1.5e-55
Identity = 119/278 (42.81%), Postives = 166/278 (59.71%), Query Frame = 1


Query: 4   ANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSSSLPIVGVHHMEAHALVARLVERE 63
           + ++  +LSA+A T+ PGL LCL VG+  +  +      P + +HHMEAHAL  RL+   
Sbjct: 93  SGISPYELSAIATTVKPGLGLCLGVGLSYSLDLVNKYHKPFIPIHHMEAHALTVRLLH-P 152

Query: 64  LQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAYDKTAKWLGLDLRK-----S 123
           ++FPF+ LLISGGH +L +   + +++ LG ++D+A G+  DK A+ L L         S
Sbjct: 153 VEFPFLVLLISGGHCILAIVSGVSEFVMLGHSLDEAPGDTLDKVARRLSLINHPQCSGMS 212

Query: 124 GGPAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLAIESRNIDTKIPLSSASI 183
           GG AIE LA  G+ K  K   PM  H+DCNFS+AGL+ QV   IE +  +  I       
Sbjct: 213 GGEAIEHLALHGNRKICKLKIPMSHHRDCNFSFAGLRNQVNKVIEQKEAEKGISKG---- 272

Query: 184 QDRSVRADIAASFQRVAVLHLEDKCERAIEWALK--IEPSIRH-LVVSGGVASNKYVRAQ 243
           Q     ADIAA+ Q    LHL  + +RAI +  +  + P+ R  LVVSGGVASN+Y+R  
Sbjct: 273 QLLPCVADIAAAVQHTVALHLAQRTQRAIYYCKREGLIPTERACLVVSGGVASNRYIRKA 332

Query: 244 LMAVVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHFRIG 274
           L  V  +  + L+CPPP LCTDNGVM+AW G+E  + G
Sbjct: 333 LQTVTHESDMTLLCPPPRLCTDNGVMIAWNGIEKLQSG 365

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BLAST of Cucsa.257530 vs. Swiss-Prot
Match: TSAD_ANAMM (tRNA N6-adenosine threonylcarbamoyltransferase OS=Anaplasma marginale (strain St. Maries) GN=tsaD PE=3 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 5.9e-55
Identity = 122/297 (41.08%), Postives = 167/297 (56.23%), Query Frame = 1


Query: 1   MEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSSSLPIVGVHHMEAHALVARLV 60
           M  A++   DLSA+AVT GPGL   L VGV  A+ IA  +  PI+ V+H+EAHALVAR++
Sbjct: 65  MGGAHIGFSDLSAIAVTSGPGLVGSLIVGVMLAKAIAYVAHKPIIAVNHLEAHALVARMI 124

Query: 61  ERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAYDKTAKWLGLDLRKSGG 120
             +L+FPF+ L+ISGGH   ++A D+G+Y +LG ++DD++GE++DK A+ LGL     GG
Sbjct: 125 YADLEFPFLVLIISGGHCQFLIAHDVGRYTKLGESVDDSLGESFDKVARMLGLGY--PGG 184

Query: 121 PAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLAIESRNIDTKIPLSSASIQD 180
           P +E  A +GD     F   +K    CNFS++GLKT VR  I           +     D
Sbjct: 185 PEVESCALKGDAHRFPFPRALKSRPGCNFSFSGLKTAVRYTI-----------AKEGKLD 244

Query: 181 RSVRADIAASFQRVAVLHLEDKCERAIEWALKIEPSIRHLVVSGGVASNKYVRAQLMAVV 240
                DI ASFQ+     L  +   A+  A   + SI  +VVSGGVA+N ++R  +    
Sbjct: 245 NQALYDICASFQQCVGEILVSRIANAMAAAKSADSSISKMVVSGGVAANNFLRGSIRKCA 304

Query: 241 RKKGLQLVCPPPSLCTDNGVMVAWTGLEHFRIGRFDPPPPADEPEDTMYDVRPRWPL 298
            + G   + PP  LCTDNG+MV W GLE+ R G F                R RWPL
Sbjct: 305 NELGFDAIFPPQELCTDNGIMVGWAGLENLRKGNF---------SSLELIPRARWPL 339

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BLAST of Cucsa.257530 vs. Swiss-Prot
Match: TSAD_RHIE6 (tRNA N6-adenosine threonylcarbamoyltransferase OS=Rhizobium etli (strain CIAT 652) GN=tsaD PE=3 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 5.9e-55
Identity = 129/298 (43.29%), Postives = 175/298 (58.72%), Query Frame = 1


Query: 3   KANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSSSLPIVGVHHMEAHALVARLVER 62
           +AN++  D+ A+A T GPGL   L VG+   + IA ++  P+  V+H+E HAL ARL + 
Sbjct: 73  RANVSLADVDAIAATSGPGLIGGLLVGLMTGKAIAKAAGKPLYAVNHLEGHALTARLTDG 132

Query: 63  ELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAYDKTAKWLGLDLRKSGGPA 122
            L FP++ LL+SGGH  L+L + +G Y + GTTIDDA+GEA+DKTAK LGL     GGPA
Sbjct: 133 -LSFPYLMLLVSGGHTQLILVRGVGDYERWGTTIDDALGEAFDKTAKLLGLPY--PGGPA 192

Query: 123 IEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLAIESRNIDTKIPLSSASIQDRS 182
           +E +AR+G+     F  P+      +FS++GLKT VRLA +            A + D+ 
Sbjct: 193 VERMARDGNAGRFDFPRPLVGEARLDFSFSGLKTAVRLAAQ----------EIAPLSDQD 252

Query: 183 VRADIAASFQRVAVLHLEDKCERAIEWALKIEPSIRH---LVVSGGVASNKYVRAQLMAV 242
           V ADI ASFQR     L+D+  R ++   +  PSI     LVV+GGVA+N  +RA L  +
Sbjct: 253 V-ADICASFQRAISRTLKDRIGRGLQRFKREFPSIGETPALVVAGGVAANLELRATLQGL 312

Query: 243 VRKKGLQLVCPPPSLCTDNGVMVAWTGLEHFRIGRFDPPPPADEPEDTMYDVRPRWPL 298
             K G + + PP SLCTDN VM+AW GLE   IG          P+      R RWPL
Sbjct: 313 CDKNGFRFIAPPLSLCTDNAVMIAWAGLERMAIG--------VAPDALDVQPRSRWPL 348

[Close]

BLAST of Cucsa.257530 vs. TrEMBL
Match: A0A0A0L938_CUCSA (Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial OS=Cucumis sativus GN=GCP1 PE=3 SV=1)

HSP 1 Score: 657.9 bits (1696), Expect = 6.5e-186
Identity = 332/332 (100.00%), Postives = 332/332 (100.00%), Query Frame = 1


Query: 1   MEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSSSLPIVGVHHMEAHALVARLV 60
           MEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSSSLPIVGVHHMEAHALVARLV
Sbjct: 155 MEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSSSLPIVGVHHMEAHALVARLV 214

Query: 61  ERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAYDKTAKWLGLDLRKSGG 120
           ERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAYDKTAKWLGLDLRKSGG
Sbjct: 215 ERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAYDKTAKWLGLDLRKSGG 274

Query: 121 PAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLAIESRNIDTKIPLSSASIQD 180
           PAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLAIESRNIDTKIPLSSASIQD
Sbjct: 275 PAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLAIESRNIDTKIPLSSASIQD 334

Query: 181 RSVRADIAASFQRVAVLHLEDKCERAIEWALKIEPSIRHLVVSGGVASNKYVRAQLMAVV 240
           RSVRADIAASFQRVAVLHLEDKCERAIEWALKIEPSIRHLVVSGGVASNKYVRAQLMAVV
Sbjct: 335 RSVRADIAASFQRVAVLHLEDKCERAIEWALKIEPSIRHLVVSGGVASNKYVRAQLMAVV 394

Query: 241 RKKGLQLVCPPPSLCTDNGVMVAWTGLEHFRIGRFDPPPPADEPEDTMYDVRPRWPLGEE 300
           RKKGLQLVCPPPSLCTDNGVMVAWTGLEHFRIGRFDPPPPADEPEDTMYDVRPRWPLGEE
Sbjct: 395 RKKGLQLVCPPPSLCTDNGVMVAWTGLEHFRIGRFDPPPPADEPEDTMYDVRPRWPLGEE 454

Query: 301 YTEGRSAARSLRTARIHPSLTSIIQASLQQQQ 333
           YTEGRSAARSLRTARIHPSLTSIIQASLQQQQ
Sbjct: 455 YTEGRSAARSLRTARIHPSLTSIIQASLQQQQ 486

[Close]

BLAST of Cucsa.257530 vs. TrEMBL
Match: D7U7W9_VITVI (Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial OS=Vitis vinifera GN=GCP1 PE=3 SV=1)

HSP 1 Score: 597.0 bits (1538), Expect = 1.4e-167
Identity = 292/331 (88.22%), Postives = 319/331 (96.37%), Query Frame = 1


Query: 1   MEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSSSLPIVGVHHMEAHALVARLV 60
           +E ANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGS +LPIVGVHHMEAHALVARL+
Sbjct: 137 LENANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSHNLPIVGVHHMEAHALVARLI 196

Query: 61  ERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAYDKTAKWLGLDLRKSGG 120
           E++LQFPFMALLISGGHNLL+LA+DLG YIQLGTTIDDAIGEAYDKTAKWLGLDLR+SGG
Sbjct: 197 EKDLQFPFMALLISGGHNLLILARDLGHYIQLGTTIDDAIGEAYDKTAKWLGLDLRRSGG 256

Query: 121 PAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLAIESRNIDTKIPLSSASIQD 180
           PAIEELAREGD K++KFSTPMKQHKDCNFSYAGLKTQVRLAIESRNI+ +IP+SSAS +D
Sbjct: 257 PAIEELAREGDAKAVKFSTPMKQHKDCNFSYAGLKTQVRLAIESRNINAEIPISSASSED 316

Query: 181 RSVRADIAASFQRVAVLHLEDKCERAIEWALKIEPSIRHLVVSGGVASNKYVRAQLMAVV 240
           RS RADIAASFQRVAVLHLE++CERAIEWALKIEPSI+HLVVSGGVASN+YVRAQL  VV
Sbjct: 317 RSSRADIAASFQRVAVLHLEERCERAIEWALKIEPSIKHLVVSGGVASNQYVRAQLDQVV 376

Query: 241 RKKGLQLVCPPPSLCTDNGVMVAWTGLEHFRIGRFDPPPPADEPEDTMYDVRPRWPLGEE 300
           +KK LQLVCPPPSLCTDNGVMVAWTGLEHFR+GR+DPPPPA+EPED +YD+RPRWPLGEE
Sbjct: 377 KKKSLQLVCPPPSLCTDNGVMVAWTGLEHFRMGRYDPPPPANEPEDYVYDLRPRWPLGEE 436

Query: 301 YTEGRSAARSLRTARIHPSLTSIIQASLQQQ 332
           Y+EGRS ARSLRTARIHPSLTS+IQAS+QQQ
Sbjct: 437 YSEGRSEARSLRTARIHPSLTSLIQASMQQQ 467

[Close]

BLAST of Cucsa.257530 vs. TrEMBL
Match: B9RD99_RICCO (Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial OS=Ricinus communis GN=GCP1 PE=3 SV=1)

HSP 1 Score: 574.3 bits (1479), Expect = 9.5e-161
Identity = 283/330 (85.76%), Postives = 311/330 (94.24%), Query Frame = 1


Query: 1   MEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSSSLPIVGVHHMEAHALVARLV 60
           ++KANLT  DLSAVAVTIGPGLSLCLRVGV+KARK+AGS +LP+VG+HHMEAHALVARLV
Sbjct: 125 LDKANLTATDLSAVAVTIGPGLSLCLRVGVRKARKLAGSFNLPMVGIHHMEAHALVARLV 184

Query: 61  ERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAYDKTAKWLGLDLRKSGG 120
           E+EL FPF+ALLISGGHNLLVLA+DLG YIQLGTTIDDAIGEAYDKTAKWLGLD+RKSGG
Sbjct: 185 EQELHFPFIALLISGGHNLLVLARDLGHYIQLGTTIDDAIGEAYDKTAKWLGLDMRKSGG 244

Query: 121 PAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLAIESRNIDTKIPLSSASIQD 180
           PAIEELAREGD +SIKFSTPM+QHKDCNFSYAGLKTQVRLAIESRNI+ +IP+S AS  D
Sbjct: 245 PAIEELAREGDAESIKFSTPMQQHKDCNFSYAGLKTQVRLAIESRNINAEIPISLASSHD 304

Query: 181 RSVRADIAASFQRVAVLHLEDKCERAIEWALKIEPSIRHLVVSGGVASNKYVRAQLMAVV 240
           RS RADIAASFQRVAVLHLE++CERAIEWA +IEPSI++LVVSGGVASN+YVRA+L  VV
Sbjct: 305 RSSRADIAASFQRVAVLHLEERCERAIEWARRIEPSIKYLVVSGGVASNQYVRARLDQVV 364

Query: 241 RKKGLQLVCPPPSLCTDNGVMVAWTGLEHFRIGRFDPPPPADEPEDTMYDVRPRWPLGEE 300
           +KK L+LVCPP SLCTDNGVMVAWTG+EHFRIGRFDPPPPADEPED MYD+RPRWPLGEE
Sbjct: 365 KKKNLKLVCPPSSLCTDNGVMVAWTGIEHFRIGRFDPPPPADEPEDIMYDLRPRWPLGEE 424

Query: 301 YTEGRSAARSLRTARIHPSLTSIIQASLQQ 331
           Y EGRS ARSL+TARIHPSLTSIIQASLQQ
Sbjct: 425 YAEGRSKARSLKTARIHPSLTSIIQASLQQ 454

[Close]

BLAST of Cucsa.257530 vs. TrEMBL
Match: B9IBY1_POPTR (Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial OS=Populus trichocarpa GN=GCP1 PE=3 SV=1)

HSP 1 Score: 573.5 bits (1477), Expect = 1.6e-160
Identity = 281/331 (84.89%), Postives = 309/331 (93.35%), Query Frame = 1


Query: 1   MEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSSSLPIVGVHHMEAHALVARLV 60
           ++KANLT  +LSAVAVTIGPGLSLCLRVGV+KARK+AGS +LPI+G+HHMEAHALVARL+
Sbjct: 134 LDKANLTATNLSAVAVTIGPGLSLCLRVGVRKARKLAGSFNLPIIGIHHMEAHALVARLI 193

Query: 61  ERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAYDKTAKWLGLDLRKSGG 120
           ERELQFPFMALLISGGHNLL+LA DLG YIQLGTTIDDAIGEAYDKTAKWLGLD+ +SGG
Sbjct: 194 ERELQFPFMALLISGGHNLLILAHDLGHYIQLGTTIDDAIGEAYDKTAKWLGLDMSRSGG 253

Query: 121 PAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLAIESRNIDTKIPLSSASIQD 180
           PA+EELAREGD +SIKFSTPMKQHKDCNFSYAGLKTQVRLAIES+NI+ +IP+SSAS QD
Sbjct: 254 PALEELAREGDAESIKFSTPMKQHKDCNFSYAGLKTQVRLAIESKNINAEIPISSASSQD 313

Query: 181 RSVRADIAASFQRVAVLHLEDKCERAIEWALKIEPSIRHLVVSGGVASNKYVRAQLMAVV 240
           R  RADIAASFQRVAVLHLE++C+RAIEWA KIEPSI+HLVVSGGVASN+YVRA+L  VV
Sbjct: 314 RRSRADIAASFQRVAVLHLEERCDRAIEWARKIEPSIKHLVVSGGVASNQYVRARLDHVV 373

Query: 241 RKKGLQLVCPPPSLCTDNGVMVAWTGLEHFRIGRFDPPPPADEPEDTMYDVRPRWPLGEE 300
           +K  LQLVCPPP LCTDNGVMVAWTG+EHF +GRFDPPPPADE ED MYD+RPRWPLGEE
Sbjct: 374 KKNSLQLVCPPPKLCTDNGVMVAWTGIEHFCMGRFDPPPPADEHEDYMYDLRPRWPLGEE 433

Query: 301 YTEGRSAARSLRTARIHPSLTSIIQASLQQQ 332
           Y EGRS ARSLRTARIHPSLTSIIQASLQQQ
Sbjct: 434 YAEGRSEARSLRTARIHPSLTSIIQASLQQQ 464

[Close]

BLAST of Cucsa.257530 vs. TrEMBL
Match: I1JKQ3_SOYBN (Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial OS=Glycine max GN=GCP1 PE=3 SV=2)

HSP 1 Score: 573.2 bits (1476), Expect = 2.1e-160
Identity = 277/330 (83.94%), Postives = 311/330 (94.24%), Query Frame = 1


Query: 1   MEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSSSLPIVGVHHMEAHALVARLV 60
           ++KA LTE+DL+AVAVTIGPGLSLCLRVGVQKARKIAG  +LPI+G+HHMEAHALVARL+
Sbjct: 161 LDKAYLTEKDLTAVAVTIGPGLSLCLRVGVQKARKIAGGFNLPIIGIHHMEAHALVARLI 220

Query: 61  ERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAYDKTAKWLGLDLRKSGG 120
           E++LQFPFMALLISGGHNLLVLA+DLGQYIQLGTTIDDAIGEAYDKTAKWLGLDLR+SGG
Sbjct: 221 EKDLQFPFMALLISGGHNLLVLARDLGQYIQLGTTIDDAIGEAYDKTAKWLGLDLRRSGG 280

Query: 121 PAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLAIESRNIDTKIPLSSASIQD 180
           PAIE+LA EG+ +S+KFS PMKQHKDCNFSYAGLKTQVRLAIES+ ID KIP+SSAS  D
Sbjct: 281 PAIEKLAMEGNAESVKFSIPMKQHKDCNFSYAGLKTQVRLAIESKKIDAKIPISSASNGD 340

Query: 181 RSVRADIAASFQRVAVLHLEDKCERAIEWALKIEPSIRHLVVSGGVASNKYVRAQLMAVV 240
           R  RADIAASFQR+AVLHLE++CERAI+WALK+EPSIRHLVVSGGVASN+YVRA+L  VV
Sbjct: 341 RLSRADIAASFQRIAVLHLEERCERAIQWALKMEPSIRHLVVSGGVASNQYVRARLDMVV 400

Query: 241 RKKGLQLVCPPPSLCTDNGVMVAWTGLEHFRIGRFDPPPPADEPEDTMYDVRPRWPLGEE 300
           +K GLQLVCPPP LCTDNGVM+AWTG+EHFR+GR+DPPPPA+EPED +YD+RPRWPLGEE
Sbjct: 401 KKNGLQLVCPPPRLCTDNGVMIAWTGIEHFRMGRYDPPPPAEEPEDFVYDIRPRWPLGEE 460

Query: 301 YTEGRSAARSLRTARIHPSLTSIIQASLQQ 331
           Y EG+S ARSLRTARIHPSLTSIIQASLQQ
Sbjct: 461 YAEGKSVARSLRTARIHPSLTSIIQASLQQ 490

[Close]

BLAST of Cucsa.257530 vs. TAIR10
Match: AT2G45270.1 (AT2G45270.1 glycoprotease 1)

HSP 1 Score: 553.1 bits (1424), Expect = 1.1e-157
Identity = 266/332 (80.12%), Postives = 303/332 (91.27%), Query Frame = 1


Query: 1   MEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSSSLPIVGVHHMEAHALVARLV 60
           ++KANLTE+DLSAVAVTIGPGLSLCLRVGV+KAR++AG+ SLPIVGVHHMEAHALVARLV
Sbjct: 146 LDKANLTEKDLSAVAVTIGPGLSLCLRVGVRKARRVAGNFSLPIVGVHHMEAHALVARLV 205

Query: 61  ERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAYDKTAKWLGLDLRKSGG 120
           E+EL FPFMALLISGGHNLLVLA  LGQY QLGTT+DDAIGEA+DKTAKWLGLD+ +SGG
Sbjct: 206 EQELSFPFMALLISGGHNLLVLAHKLGQYTQLGTTVDDAIGEAFDKTAKWLGLDMHRSGG 265

Query: 121 PAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLAIESRNIDTKIPLSSASIQD 180
           PA+EELA EGD KS+KF+ PMK HKDCNFSYAGLKTQVRLAIE++ ID K P+SSA+ +D
Sbjct: 266 PAVEELALEGDAKSVKFNVPMKYHKDCNFSYAGLKTQVRLAIEAKEIDAKCPVSSATNED 325

Query: 181 RSVRADIAASFQRVAVLHLEDKCERAIEWALKIEPSIRHLVVSGGVASNKYVRAQLMAVV 240
           R  RADIAASFQRVAVLHLE+KCERAI+WAL++EPSI+H+V+SGGVASNKYVR +L  +V
Sbjct: 326 RRNRADIAASFQRVAVLHLEEKCERAIDWALELEPSIKHMVISGGVASNKYVRLRLNNIV 385

Query: 241 RKKGLQLVCPPPSLCTDNGVMVAWTGLEHFRIGRFDPPPPADEPEDTMYDVRPRWPLGEE 300
             K L+LVCPPPSLCTDNGVMVAWTGLEHFR+GR+DPPPPA EPED +YD+RPRWPLGEE
Sbjct: 386 ENKNLKLVCPPPSLCTDNGVMVAWTGLEHFRVGRYDPPPPATEPEDYVYDLRPRWPLGEE 445

Query: 301 YTEGRSAARSLRTARIHPSLTSIIQASLQQQQ 333
           Y +GRS ARS+RTARIHPSLTSII+A   QQQ
Sbjct: 446 YAKGRSEARSMRTARIHPSLTSIIRADSLQQQ 477

[Close]

BLAST of Cucsa.257530 vs. TAIR10
Match: AT4G22720.1 (AT4G22720.1 Actin-like ATPase superfamily protein)

HSP 1 Score: 89.4 bits (220), Expect = 4.7e-18
Identity = 74/270 (27.41%), Postives = 123/270 (45.56%), Query Frame = 1


Query: 1   MEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSSSLPIVGVHHMEAHALVARLV 60
           +E + +T  ++  +  T GPG+   L+V     R ++     PIV V+H  AH  + R+V
Sbjct: 65  LETSQVTPEEIDCICYTKGPGMGAPLQVSAIVVRVLSQLWKKPIVAVNHCVAHIEMGRVV 124

Query: 61  ERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAYDKTAKWLGLDLRKSGG 120
                 P + L +SGG N  V+A   G+Y   G TID A+G   D+ A+ L L    S G
Sbjct: 125 TGA-DDP-VVLYVSGG-NTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLKLSNDPSPG 184

Query: 121 PAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLAIESRNIDTKIPLSSASIQD 180
             IE+LA++G+     F       K  + S++G+ + +    E +              +
Sbjct: 185 YNIEQLAKKGE----NFIDLPYAVKGMDVSFSGILSYIETTAEEK-----------LKNN 244

Query: 181 RSVRADIAASFQRVAVLHLEDKCERAIEWALKIEPSIRHLVVSGGVASNKYVRAQLMAVV 240
               AD+  S Q      L +  ERA+    K     + +++ GGV  N+ ++  +  + 
Sbjct: 245 ECTPADLCYSLQETVFAMLVEITERAMAHCDK-----KDVLIVGGVGCNERLQEMMRTMC 304

Query: 241 RKKGLQLVCPPPSLCTDNGVMVAWTGLEHF 271
            ++  +L       C DNG M+A+TGL  F
Sbjct: 305 SERDGKLFATDDRYCIDNGAMIAYTGLLAF 311

[Close]

BLAST of Cucsa.257530 vs. NCBI nr
Match: gi|700201478|gb|KGN56611.1| (hypothetical protein Csa_3G126750 [Cucumis sativus])

HSP 1 Score: 657.9 bits (1696), Expect = 9.4e-186
Identity = 332/332 (100.00%), Postives = 332/332 (100.00%), Query Frame = 1


Query: 1   MEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSSSLPIVGVHHMEAHALVARLV 60
           MEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSSSLPIVGVHHMEAHALVARLV
Sbjct: 155 MEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSSSLPIVGVHHMEAHALVARLV 214

Query: 61  ERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAYDKTAKWLGLDLRKSGG 120
           ERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAYDKTAKWLGLDLRKSGG
Sbjct: 215 ERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAYDKTAKWLGLDLRKSGG 274

Query: 121 PAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLAIESRNIDTKIPLSSASIQD 180
           PAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLAIESRNIDTKIPLSSASIQD
Sbjct: 275 PAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLAIESRNIDTKIPLSSASIQD 334

Query: 181 RSVRADIAASFQRVAVLHLEDKCERAIEWALKIEPSIRHLVVSGGVASNKYVRAQLMAVV 240
           RSVRADIAASFQRVAVLHLEDKCERAIEWALKIEPSIRHLVVSGGVASNKYVRAQLMAVV
Sbjct: 335 RSVRADIAASFQRVAVLHLEDKCERAIEWALKIEPSIRHLVVSGGVASNKYVRAQLMAVV 394

Query: 241 RKKGLQLVCPPPSLCTDNGVMVAWTGLEHFRIGRFDPPPPADEPEDTMYDVRPRWPLGEE 300
           RKKGLQLVCPPPSLCTDNGVMVAWTGLEHFRIGRFDPPPPADEPEDTMYDVRPRWPLGEE
Sbjct: 395 RKKGLQLVCPPPSLCTDNGVMVAWTGLEHFRIGRFDPPPPADEPEDTMYDVRPRWPLGEE 454

Query: 301 YTEGRSAARSLRTARIHPSLTSIIQASLQQQQ 333
           YTEGRSAARSLRTARIHPSLTSIIQASLQQQQ
Sbjct: 455 YTEGRSAARSLRTARIHPSLTSIIQASLQQQQ 486

[Close]

BLAST of Cucsa.257530 vs. NCBI nr
Match: gi|778677465|ref|XP_011650793.1| (PREDICTED: probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial [Cucumis sativus])

HSP 1 Score: 657.9 bits (1696), Expect = 9.4e-186
Identity = 332/332 (100.00%), Postives = 332/332 (100.00%), Query Frame = 1


Query: 1   MEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSSSLPIVGVHHMEAHALVARLV 60
           MEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSSSLPIVGVHHMEAHALVARLV
Sbjct: 137 MEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSSSLPIVGVHHMEAHALVARLV 196

Query: 61  ERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAYDKTAKWLGLDLRKSGG 120
           ERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAYDKTAKWLGLDLRKSGG
Sbjct: 197 ERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAYDKTAKWLGLDLRKSGG 256

Query: 121 PAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLAIESRNIDTKIPLSSASIQD 180
           PAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLAIESRNIDTKIPLSSASIQD
Sbjct: 257 PAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLAIESRNIDTKIPLSSASIQD 316

Query: 181 RSVRADIAASFQRVAVLHLEDKCERAIEWALKIEPSIRHLVVSGGVASNKYVRAQLMAVV 240
           RSVRADIAASFQRVAVLHLEDKCERAIEWALKIEPSIRHLVVSGGVASNKYVRAQLMAVV
Sbjct: 317 RSVRADIAASFQRVAVLHLEDKCERAIEWALKIEPSIRHLVVSGGVASNKYVRAQLMAVV 376

Query: 241 RKKGLQLVCPPPSLCTDNGVMVAWTGLEHFRIGRFDPPPPADEPEDTMYDVRPRWPLGEE 300
           RKKGLQLVCPPPSLCTDNGVMVAWTGLEHFRIGRFDPPPPADEPEDTMYDVRPRWPLGEE
Sbjct: 377 RKKGLQLVCPPPSLCTDNGVMVAWTGLEHFRIGRFDPPPPADEPEDTMYDVRPRWPLGEE 436

Query: 301 YTEGRSAARSLRTARIHPSLTSIIQASLQQQQ 333
           YTEGRSAARSLRTARIHPSLTSIIQASLQQQQ
Sbjct: 437 YTEGRSAARSLRTARIHPSLTSIIQASLQQQQ 468

[Close]

BLAST of Cucsa.257530 vs. NCBI nr
Match: gi|659075502|ref|XP_008438179.1| (PREDICTED: probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial [Cucumis melo])

HSP 1 Score: 654.1 bits (1686), Expect = 1.4e-184
Identity = 330/332 (99.40%), Postives = 330/332 (99.40%), Query Frame = 1


Query: 1   MEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSSSLPIVGVHHMEAHALVARLV 60
           MEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSSSLPIVGVHHMEAHALVARLV
Sbjct: 134 MEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSSSLPIVGVHHMEAHALVARLV 193

Query: 61  ERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAYDKTAKWLGLDLRKSGG 120
           ERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAYDKTAKWLGLDLRKSGG
Sbjct: 194 ERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAYDKTAKWLGLDLRKSGG 253

Query: 121 PAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLAIESRNIDTKIPLSSASIQD 180
           PAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLAIESRNIDTKIPLSSASIQD
Sbjct: 254 PAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLAIESRNIDTKIPLSSASIQD 313

Query: 181 RSVRADIAASFQRVAVLHLEDKCERAIEWALKIEPSIRHLVVSGGVASNKYVRAQLMAVV 240
           RSVRADIAASFQRVAVLHLEDKCERAIEWALKIEPSIRHLVVSGGVASNKYVRAQL AV 
Sbjct: 314 RSVRADIAASFQRVAVLHLEDKCERAIEWALKIEPSIRHLVVSGGVASNKYVRAQLTAVA 373

Query: 241 RKKGLQLVCPPPSLCTDNGVMVAWTGLEHFRIGRFDPPPPADEPEDTMYDVRPRWPLGEE 300
           RKKGLQLVCPPPSLCTDNGVMVAWTGLEHFRIGRFDPPPPADEPEDTMYDVRPRWPLGEE
Sbjct: 374 RKKGLQLVCPPPSLCTDNGVMVAWTGLEHFRIGRFDPPPPADEPEDTMYDVRPRWPLGEE 433

Query: 301 YTEGRSAARSLRTARIHPSLTSIIQASLQQQQ 333
           YTEGRSAARSLRTARIHPSLTSIIQASLQQQQ
Sbjct: 434 YTEGRSAARSLRTARIHPSLTSIIQASLQQQQ 465

[Close]

BLAST of Cucsa.257530 vs. NCBI nr
Match: gi|225454005|ref|XP_002280713.1| (PREDICTED: probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial isoform X1 [Vitis vinifera])

HSP 1 Score: 597.0 bits (1538), Expect = 2.0e-167
Identity = 292/331 (88.22%), Postives = 319/331 (96.37%), Query Frame = 1


Query: 1   MEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSSSLPIVGVHHMEAHALVARLV 60
           +E ANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGS +LPIVGVHHMEAHALVARL+
Sbjct: 137 LENANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSHNLPIVGVHHMEAHALVARLI 196

Query: 61  ERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAYDKTAKWLGLDLRKSGG 120
           E++LQFPFMALLISGGHNLL+LA+DLG YIQLGTTIDDAIGEAYDKTAKWLGLDLR+SGG
Sbjct: 197 EKDLQFPFMALLISGGHNLLILARDLGHYIQLGTTIDDAIGEAYDKTAKWLGLDLRRSGG 256

Query: 121 PAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLAIESRNIDTKIPLSSASIQD 180
           PAIEELAREGD K++KFSTPMKQHKDCNFSYAGLKTQVRLAIESRNI+ +IP+SSAS +D
Sbjct: 257 PAIEELAREGDAKAVKFSTPMKQHKDCNFSYAGLKTQVRLAIESRNINAEIPISSASSED 316

Query: 181 RSVRADIAASFQRVAVLHLEDKCERAIEWALKIEPSIRHLVVSGGVASNKYVRAQLMAVV 240
           RS RADIAASFQRVAVLHLE++CERAIEWALKIEPSI+HLVVSGGVASN+YVRAQL  VV
Sbjct: 317 RSSRADIAASFQRVAVLHLEERCERAIEWALKIEPSIKHLVVSGGVASNQYVRAQLDQVV 376

Query: 241 RKKGLQLVCPPPSLCTDNGVMVAWTGLEHFRIGRFDPPPPADEPEDTMYDVRPRWPLGEE 300
           +KK LQLVCPPPSLCTDNGVMVAWTGLEHFR+GR+DPPPPA+EPED +YD+RPRWPLGEE
Sbjct: 377 KKKSLQLVCPPPSLCTDNGVMVAWTGLEHFRMGRYDPPPPANEPEDYVYDLRPRWPLGEE 436

Query: 301 YTEGRSAARSLRTARIHPSLTSIIQASLQQQ 332
           Y+EGRS ARSLRTARIHPSLTS+IQAS+QQQ
Sbjct: 437 YSEGRSEARSLRTARIHPSLTSLIQASMQQQ 467

[Close]

BLAST of Cucsa.257530 vs. NCBI nr
Match: gi|731421239|ref|XP_010661663.1| (PREDICTED: probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial isoform X2 [Vitis vinifera])

HSP 1 Score: 597.0 bits (1538), Expect = 2.0e-167
Identity = 292/331 (88.22%), Postives = 319/331 (96.37%), Query Frame = 1


Query: 1   MEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSSSLPIVGVHHMEAHALVARLV 60
           +E ANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGS +LPIVGVHHMEAHALVARL+
Sbjct: 18  LENANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSHNLPIVGVHHMEAHALVARLI 77

Query: 61  ERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAYDKTAKWLGLDLRKSGG 120
           E++LQFPFMALLISGGHNLL+LA+DLG YIQLGTTIDDAIGEAYDKTAKWLGLDLR+SGG
Sbjct: 78  EKDLQFPFMALLISGGHNLLILARDLGHYIQLGTTIDDAIGEAYDKTAKWLGLDLRRSGG 137

Query: 121 PAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLAIESRNIDTKIPLSSASIQD 180
           PAIEELAREGD K++KFSTPMKQHKDCNFSYAGLKTQVRLAIESRNI+ +IP+SSAS +D
Sbjct: 138 PAIEELAREGDAKAVKFSTPMKQHKDCNFSYAGLKTQVRLAIESRNINAEIPISSASSED 197

Query: 181 RSVRADIAASFQRVAVLHLEDKCERAIEWALKIEPSIRHLVVSGGVASNKYVRAQLMAVV 240
           RS RADIAASFQRVAVLHLE++CERAIEWALKIEPSI+HLVVSGGVASN+YVRAQL  VV
Sbjct: 198 RSSRADIAASFQRVAVLHLEERCERAIEWALKIEPSIKHLVVSGGVASNQYVRAQLDQVV 257

Query: 241 RKKGLQLVCPPPSLCTDNGVMVAWTGLEHFRIGRFDPPPPADEPEDTMYDVRPRWPLGEE 300
           +KK LQLVCPPPSLCTDNGVMVAWTGLEHFR+GR+DPPPPA+EPED +YD+RPRWPLGEE
Sbjct: 258 KKKSLQLVCPPPSLCTDNGVMVAWTGLEHFRMGRYDPPPPANEPEDYVYDLRPRWPLGEE 317

Query: 301 YTEGRSAARSLRTARIHPSLTSIIQASLQQQ 332
           Y+EGRS ARSLRTARIHPSLTS+IQAS+QQQ
Sbjct: 318 YSEGRSEARSLRTARIHPSLTSLIQASMQQQ 348

The following BLAST results are available for this feature:

BLAST of Cucsa.257530 vs. Swiss-Prot


Match Name	E-value	Identity	Description
OSGP2_ARATH	2.0e-156	80.12	Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial OS=Arabid...	[more]
TSAD_GLUOX	1.2e-55	43.19	tRNA N6-adenosine threonylcarbamoyltransferase OS=Gluconobacter oxydans (strain ...	[more]
OSGP2_XENTR	1.5e-55	42.81	Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial OS=Xenopu...	[more]
TSAD_ANAMM	5.9e-55	41.08	tRNA N6-adenosine threonylcarbamoyltransferase OS=Anaplasma marginale (strain St...	[more]
TSAD_RHIE6	5.9e-55	43.29	tRNA N6-adenosine threonylcarbamoyltransferase OS=Rhizobium etli (strain CIAT 65...	[more]

BLAST of Cucsa.257530 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L938_CUCSA	6.5e-186	100.00	Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial OS=Cucumi...	[more]
D7U7W9_VITVI	1.4e-167	88.22	Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial OS=Vitis ...	[more]
B9RD99_RICCO	9.5e-161	85.76	Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial OS=Ricinu...	[more]
B9IBY1_POPTR	1.6e-160	84.89	Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial OS=Populu...	[more]
I1JKQ3_SOYBN	2.1e-160	83.94	Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial OS=Glycin...	[more]

BLAST of Cucsa.257530 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G45270.1	1.1e-157	80.12	glycoprotease 1	[more]
AT4G22720.1	4.7e-18	27.41	Actin-like ATPase superfamily protein	[more]

BLAST of Cucsa.257530 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700201478\|gb\|KGN56611.1\|	9.4e-186	100.00	hypothetical protein Csa_3G126750 [Cucumis sativus]	[more]
gi\|778677465\|ref\|XP_011650793.1\|	9.4e-186	100.00	PREDICTED: probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondria...	[more]
gi\|659075502\|ref\|XP_008438179.1\|	1.4e-184	99.40	PREDICTED: probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondria...	[more]
gi\|225454005\|ref\|XP_002280713.1\|	2.0e-167	88.22	PREDICTED: probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondria...	[more]
gi\|731421239\|ref\|XP_010661663.1\|	2.0e-167	88.22	PREDICTED: probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondria...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR000905	Gcp-like_dom
IPR017861	KAE1/TsaD

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009793	embryo development ending in seed dormancy
biological_process	GO:0016556	mRNA modification
biological_process	GO:0006508	proteolysis
biological_process	GO:0002949	tRNA threonylcarbamoyladenosine modification
cellular_component	GO:0005743	mitochondrial inner membrane
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004222	metalloendopeptidase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsa.257530.1	Cucsa.257530.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000905	Gcp-like domain	PFAM	PF00814	Peptidase_M22	coord: 1..264 score: 6.8
IPR017861	Kae1/TsaD family	PRINTS	PR00789	OSIALOPTASE	coord: 92..113 score: 5.6E-36coord: 220..229 score: 5.6E-36coord: 14..34 score: 5.6E-36coord: 35..54 score: 5.6E-36coord: 69..81 score: 5.6
IPR017861	Kae1/TsaD family	TIGRFAMs	TIGR00329	TIGR00329	coord: 2..263 score: 7.8
None	No IPR available	GENE3D	G3DSA:3.30.420.40		coord: 1..97 score: 4.3
None	No IPR available	PANTHER	PTHR11735	O-SIALOGLYCOPROTEIN ENDOPEPTIDASE	coord: 1..329 score: 3.8E
None	No IPR available	PANTHER	PTHR11735:SF12	TRNA N6-ADENOSINE THREONYLCARBAMOYLTRANSFERASE, MITOCHONDRIAL-RELATED	coord: 1..329 score: 3.8E
None	No IPR available	unknown	SSF53067	Actin-like ATPase domain	coord: 1..60 score: 2.04E-13coord: 41..266 score: 2.78

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cucsa.257530	Cla021320	Watermelon (97103) v1	cgywmB479
Cucsa.257530	Csa3G126750	Cucumber (Chinese Long) v2	cgycuB366
Cucsa.257530	MELO3C006202	Melon (DHL92) v3.5.1	cgymeB439
Cucsa.257530	ClCG05G002370	Watermelon (Charleston Gray)	cgywcgB451
Cucsa.257530	CSPI03G09680	Wild cucumber (PI 183967)	cgycpiB383
Cucsa.257530	CmaCh14G019690	Cucurbita maxima (Rimu)	cgycmaB0709
Cucsa.257530	CmoCh14G020300	Cucurbita moschata (Rifu)	cgycmoB0700
Cucsa.257530	Lsi05G019490	Bottle gourd (USVL1VR-Ls)	cgylsiB415
Cucsa.257530	Cp4.1LG03g17370	Cucurbita pepo (Zucchini)	cgycpeB0682
Cucsa.257530	MELO3C006202.2	Melon (DHL92) v3.6.1	cgymedB432
Cucsa.257530	CsaV3_3G009810	Cucumber (Chinese Long) v3	cgycucB387
Cucsa.257530	Cla97C05G082880	Watermelon (97103) v2	cgywmbB452
Cucsa.257530	BhiUN737G2	Wax gourd	cgywgoB513
Cucsa.257530	Carg14847	Silver-seed gourd	carcgyB0693

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cucsa.257530	Watermelon (Charleston Gray)	cgywcgB454
Cucsa.257530	Watermelon (97103) v1	cgywmB484
Cucsa.257530	Cucurbita pepo (Zucchini)	cgycpeB0670
Cucsa.257530	Cucurbita pepo (Zucchini)	cgycpeB0677
Cucsa.257530	Cucurbita pepo (Zucchini)	cgycpeB0679
Cucsa.257530	Cucurbita pepo (Zucchini)	cgycpeB0687
Cucsa.257530	Bottle gourd (USVL1VR-Ls)	cgylsiB410
Cucsa.257530	Melon (DHL92) v3.6.1	cgymedB423
Cucsa.257530	Silver-seed gourd	carcgyB1077
Cucsa.257530	Silver-seed gourd	carcgyB0269
Cucsa.257530	Silver-seed gourd	carcgyB1191
Cucsa.257530	Cucumber (Chinese Long) v3	cgycucB398
Cucsa.257530	Watermelon (97103) v2	cgywmbB455
Cucsa.257530	Wax gourd	cgywgoB506
Cucsa.257530	Cucumber (Gy14) v1	cgycgyB130
Cucsa.257530	Cucurbita maxima (Rimu)	cgycmaB0715
Cucsa.257530	Cucurbita maxima (Rimu)	cgycmaB0727
Cucsa.257530	Cucurbita maxima (Rimu)	cgycmaB0730
Cucsa.257530	Cucurbita moschata (Rifu)	cgycmoB0708
Cucsa.257530	Cucurbita moschata (Rifu)	cgycmoB0718
Cucsa.257530	Cucurbita moschata (Rifu)	cgycmoB0719
Cucsa.257530	Wild cucumber (PI 183967)	cgycpiB393
Cucsa.257530	Cucumber (Chinese Long) v2	cgycuB374
Cucsa.257530	Melon (DHL92) v3.5.1	cgymeB429