Cucsa.256700 (gene) Cucumber (Gy14) v1

NameCucsa.256700
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionHeat shock transcription factor A5a
Locationscaffold02229 : 4249847 .. 4252088 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AACCTCACCTGTCGATTACACACCAAAATCAATTTCCCACCATCTTCGTCATCTCCCTCCGCCTAATCTTCATCTCCAATTCCATTTCACCCGCTTCCGGCCATCGATTCACATCCCGATCCGCCGTTCTTCTCATCTAATGGAGGCGGCTGCTGCCGGCGGAGGCACCGGCGGCGCTGGTCCAGCGCCGTTTCTCATCAAAACTTACGATATGGTTGACGACTCTTCCACCGATGAGATCGTCTCGTGGACCTCTTCTAAGAAGAGCTTTGTGGTTTGGAATCCTCCTGAATTCGCCCGCCTTCTTCTTCCAACGTTTTTCAAGCACAGCAACTTCTCCAGCTTCATTCGGCAGCTTAATACCTATGTAAGTTTCTCGAGATGCCTCTTTCGATGACTTCTATCGTCACAATGATTTTATTTTGCTCCTCCTTTTAATTGGGTGTGGAGAATTTTCCATGTTGCGTAATTATGAACTTCATTGCTGTTTTCTCCATCAGTTACTTACTACGTGTTAATACATTTGTCTTATGTGTTTCGGCAATTCTTGATTGTTCTGTATTCATTCGTAGCTTCAACTGATGTAATCCGTTGGGATTTAGCTATTTACTTACTTACTACGAGTTGTGTTGATATCTAATTTACATGTGTTAATTGTATTCCTAGCTTCGAGATTATTAATTAAATTGTTGGTTTTCTGAGAAAAAAGTTCTACTAGATCTCTGTAGTTGAGGTACTACTACAAGAGAATTATCTCCACTTTCCCTTGCACTCCCAATTGAATGTTATGTGATTCGTCTACTCATAAATGAATGGAGATTTGGCTGGAACTTGTTTTGCTGGGTACTATATAATTTCTTTATCTCTAGATGGTCTTTATGTACTGGGAATTATCTTTCCATATGTTATGCTGAAATCTATTGAATGGTTTCTGTTGTACACATAGTTTTATAAGAAAATTGTTTCATTCTCTTGTTTTGAAAAGCTGAACCGACCAATTTCATAATCACAGTAACTTGCAAATGAAGTAGCCTAAACTTTCCAGTTTTGAAAATTTTGATGGCAGGGGTTTCGGAAGATCGATTCTGAGAAGTGGGAATTTGCAAATGAAGATTTTATAAAGGATCAAAAGCACCTGCTGAAAAACATCCACCGTCGGAAACCAATTCACAGTCACAGTAATCCTCAAGGATCTCACATCGATCCAGAAAGAGCTGCTTTTGAAGATGAAATAGAGAGGCTAGCACGTGAAAAAACTACACTTGAAACCAACATATCAAGATTCAAACAGCAAAAATCAACTGCTAAACTTCAGTTGCAAGATCTAACGGTGAAGGTGGAAAGCATGGAAAAAAGGCAGAAGAATTTATTGGCATTTTTAGAAAAGGCTGTTCAGAACCCTTCTTTTGTTGAACATCTTGCTCGAAGGGTCGAGTCTATGGACTTTACAGCATTTAAGAAGAAGAGACGACTGCCTTCAGCTGATCTTTCACAGCCAGTCGTAGAAAATAGTTTTTTGGATAACCATAGTAGTTCCAGATCTGAGTCTGGGAATATTTTTCATCAAGATTTTTCACAAAAGCTAAGATTAGAAACCTCATGTGCATCAGATATCAACTTAATTTCACGTAGCACCCAGAGTTCAAACGAAGAAGGGGGCAGTTCACAAAGACAGTTGTCAAAGTTTGATACTAGAGCTGTCCAAGAAAATATTCATTTTGCAGTTGAAACACTAGATCTCTCAGACACTGGGACATCGTTTATATTGAGAAGGGATTCATCTTTGTCAGGGAAATCACACAATGACGATAGTCCATGTCTACACTCGTTACAACCAAGTGTTAGTTCCAAAGAAGATGGAGAAAGTCACATCTCATGTCAATTAAATCTCACTCTTGCATCGTCTTCCCTGCGAATCAATGACACTGCTTGTTCAGTTCGAATGCCACAACTGGGTCAAAATGTTCGAAAATTCCCAGATTCAAAAGTAAATTCAAATGGAAAGGAATCAGATGTGAGACTGTTCACTAAAAACATAAATCTAGACGAGGGGAGTACTCCAGTTTGTCCTCAAGAGACCTCCAATAACAATCACGGCCCTCCAGCTGCTTCTATCAGGGCCAATGATGTTTTCTGGGAACGGTTACTTACTGAAAGACCGGGCTGTCCTGAAAGTGAAGAGGCAAGTTCCAATTACAGAGCGAATCCGTTCAAGGAGCCAGATGATGGGACTGTTCACTAA

mRNA sequence

AACCTCACCTGTCGATTACACACCAAAATCAATTTCCCACCATCTTCGTCATCTCCCTCCGCCTAATCTTCATCTCCAATTCCATTTCACCCGCTTCCGGCCATCGATTCACATCCCGATCCGCCGTTCTTCTCATCTAATGGAGGCGGCTGCTGCCGGCGGAGGCACCGGCGGCGCTGGTCCAGCGCCGTTTCTCATCAAAACTTACGATATGGTTGACGACTCTTCCACCGATGAGATCGTCTCGTGGACCTCTTCTAAGAAGAGCTTTGTGGTTTGGAATCCTCCTGAATTCGCCCGCCTTCTTCTTCCAACGTTTTTCAAGCACAGCAACTTCTCCAGCTTCATTCGGCAGCTTAATACCTATGGGTTTCGGAAGATCGATTCTGAGAAGTGGGAATTTGCAAATGAAGATTTTATAAAGGATCAAAAGCACCTGCTGAAAAACATCCACCGTCGGAAACCAATTCACAGTCACAGTAATCCTCAAGGATCTCACATCGATCCAGAAAGAGCTGCTTTTGAAGATGAAATAGAGAGGCTAGCACGTGAAAAAACTACACTTGAAACCAACATATCAAGATTCAAACAGCAAAAATCAACTGCTAAACTTCAGTTGCAAGATCTAACGGTGAAGGTGGAAAGCATGGAAAAAAGGCAGAAGAATTTATTGGCATTTTTAGAAAAGGCTGTTCAGAACCCTTCTTTTGTTGAACATCTTGCTCGAAGGGTCGAGTCTATGGACTTTACAGCATTTAAGAAGAAGAGACGACTGCCTTCAGCTGATCTTTCACAGCCAGTCGTAGAAAATAGTTTTTTGGATAACCATAGTAGTTCCAGATCTGAGTCTGGGAATATTTTTCATCAAGATTTTTCACAAAAGCTAAGATTAGAAACCTCATGTGCATCAGATATCAACTTAATTTCACGTAGCACCCAGAGTTCAAACGAAGAAGGGGGCAGTTCACAAAGACAGTTGTCAAAGTTTGATACTAGAGCTGTCCAAGAAAATATTCATTTTGCAGTTGAAACACTAGATCTCTCAGACACTGGGACATCGTTTATATTGAGAAGGGATTCATCTTTGTCAGGGAAATCACACAATGACGATAGTCCATGTCTACACTCGTTACAACCAAGTGTTAGTTCCAAAGAAGATGGAGAAAGTCACATCTCATGTCAATTAAATCTCACTCTTGCATCGTCTTCCCTGCGAATCAATGACACTGCTTGTTCAGTTCGAATGCCACAACTGGGTCAAAATGTTCGAAAATTCCCAGATTCAAAAGTAAATTCAAATGGAAAGGAATCAGATGTGAGACTGTTCACTAAAAACATAAATCTAGACGAGGGGAGTACTCCAGTTTGTCCTCAAGAGACCTCCAATAACAATCACGGCCCTCCAGCTGCTTCTATCAGGGCCAATGATGTTTTCTGGGAACGGTTACTTACTGAAAGACCGGGCTGTCCTGAAAGTGAAGAGGCAAGTTCCAATTACAGAGCGAATCCGTTCAAGGAGCCAGATGATGGGACTGTTCACTAA

Coding sequence (CDS)

ATGGAGGCGGCTGCTGCCGGCGGAGGCACCGGCGGCGCTGGTCCAGCGCCGTTTCTCATCAAAACTTACGATATGGTTGACGACTCTTCCACCGATGAGATCGTCTCGTGGACCTCTTCTAAGAAGAGCTTTGTGGTTTGGAATCCTCCTGAATTCGCCCGCCTTCTTCTTCCAACGTTTTTCAAGCACAGCAACTTCTCCAGCTTCATTCGGCAGCTTAATACCTATGGGTTTCGGAAGATCGATTCTGAGAAGTGGGAATTTGCAAATGAAGATTTTATAAAGGATCAAAAGCACCTGCTGAAAAACATCCACCGTCGGAAACCAATTCACAGTCACAGTAATCCTCAAGGATCTCACATCGATCCAGAAAGAGCTGCTTTTGAAGATGAAATAGAGAGGCTAGCACGTGAAAAAACTACACTTGAAACCAACATATCAAGATTCAAACAGCAAAAATCAACTGCTAAACTTCAGTTGCAAGATCTAACGGTGAAGGTGGAAAGCATGGAAAAAAGGCAGAAGAATTTATTGGCATTTTTAGAAAAGGCTGTTCAGAACCCTTCTTTTGTTGAACATCTTGCTCGAAGGGTCGAGTCTATGGACTTTACAGCATTTAAGAAGAAGAGACGACTGCCTTCAGCTGATCTTTCACAGCCAGTCGTAGAAAATAGTTTTTTGGATAACCATAGTAGTTCCAGATCTGAGTCTGGGAATATTTTTCATCAAGATTTTTCACAAAAGCTAAGATTAGAAACCTCATGTGCATCAGATATCAACTTAATTTCACGTAGCACCCAGAGTTCAAACGAAGAAGGGGGCAGTTCACAAAGACAGTTGTCAAAGTTTGATACTAGAGCTGTCCAAGAAAATATTCATTTTGCAGTTGAAACACTAGATCTCTCAGACACTGGGACATCGTTTATATTGAGAAGGGATTCATCTTTGTCAGGGAAATCACACAATGACGATAGTCCATGTCTACACTCGTTACAACCAAGTGTTAGTTCCAAAGAAGATGGAGAAAGTCACATCTCATGTCAATTAAATCTCACTCTTGCATCGTCTTCCCTGCGAATCAATGACACTGCTTGTTCAGTTCGAATGCCACAACTGGGTCAAAATGTTCGAAAATTCCCAGATTCAAAAGTAAATTCAAATGGAAAGGAATCAGATGTGAGACTGTTCACTAAAAACATAAATCTAGACGAGGGGAGTACTCCAGTTTGTCCTCAAGAGACCTCCAATAACAATCACGGCCCTCCAGCTGCTTCTATCAGGGCCAATGATGTTTTCTGGGAACGGTTACTTACTGAAAGACCGGGCTGTCCTGAAAGTGAAGAGGCAAGTTCCAATTACAGAGCGAATCCGTTCAAGGAGCCAGATGATGGGACTGTTCACTAA

Protein sequence

MEAAAAGGGTGGAGPAPFLIKTYDMVDDSSTDEIVSWTSSKKSFVVWNPPEFARLLLPTFFKHSNFSSFIRQLNTYGFRKIDSEKWEFANEDFIKDQKHLLKNIHRRKPIHSHSNPQGSHIDPERAAFEDEIERLAREKTTLETNISRFKQQKSTAKLQLQDLTVKVESMEKRQKNLLAFLEKAVQNPSFVEHLARRVESMDFTAFKKKRRLPSADLSQPVVENSFLDNHSSSRSESGNIFHQDFSQKLRLETSCASDINLISRSTQSSNEEGGSSQRQLSKFDTRAVQENIHFAVETLDLSDTGTSFILRRDSSLSGKSHNDDSPCLHSLQPSVSSKEDGESHISCQLNLTLASSSLRINDTACSVRMPQLGQNVRKFPDSKVNSNGKESDVRLFTKNINLDEGSTPVCPQETSNNNHGPPAASIRANDVFWERLLTERPGCPESEEASSNYRANPFKEPDDGTVH*
BLAST of Cucsa.256700 vs. Swiss-Prot
Match: HSFA5_ARATH (Heat stress transcription factor A-5 OS=Arabidopsis thaliana GN=HSFA5 PE=2 SV=1)

HSP 1 Score: 438.0 bits (1125), Expect = 1.3e-121
Identity = 240/466 (51.50%), Postives = 314/466 (67.38%), Query Frame = 1

Query: 3   AAAAGGGTGGAGPAPFLIKTYDMVDDSSTDEIVSWTSSKKSFVVWNPPEFARLLLPTFFK 62
           +A+  GG G  GPAPFL+KTY+MVDDSSTD+IVSW+++  SF+VWN  EF+RLLLPT+FK
Sbjct: 9   SASVSGGEGAGGPAPFLVKTYEMVDDSSTDQIVSWSANNNSFIVWNHAEFSRLLLPTYFK 68

Query: 63  HSNFSSFIRQLNTYGFRKIDSEKWEFANEDFIKDQKHLLKNIHRRKPIHSHSNPQGSHID 122
           H+NFSSFIRQLNTYGFRKID E+WEF N+DFIKDQKHLLKNIHRRKPIHSHS+P  S  D
Sbjct: 69  HNNFSSFIRQLNTYGFRKIDPERWEFLNDDFIKDQKHLLKNIHRRKPIHSHSHPPASSTD 128

Query: 123 PERAAFEDEIERLAREKTTLETNISRFKQQKSTAKLQLQDLTVKVESMEKRQKNLLAFLE 182
            ERA  ++++++L+REK  +E  + +FKQQK  AK Q +++T  V+ ME RQK LL FLE
Sbjct: 129 QERAVLQEQMDKLSREKAAIEAKLLKFKQQKVVAKHQFEEMTEHVDDMENRQKKLLNFLE 188

Query: 183 KAVQNPSFVEHLARRVESMDFTAFKKKRRLPSADLSQPVVENSFLDNHS-SSRSESGNIF 242
            A++NP+FV++  ++VE +D +A+ KKRRLP  + S+P  E+S LDN S SSR ESGNIF
Sbjct: 189 TAIRNPTFVKNFGKKVEQLDISAYNKKRRLPEVEQSKPPSEDSHLDNSSGSSRRESGNIF 248

Query: 243 HQDFSQKLRLETSCA-SDINLISRSTQSSNEEGGSSQRQLSKFD---TRAVQENIHFAVE 302
           HQ+FS KLRLE S A SD+N++S S QSSNEEG S +  LS  D   T   +E + FA E
Sbjct: 249 HQNFSNKLRLELSPADSDMNMVSHSIQSSNEEGASPKGILSGGDPNTTLTKREGLPFAPE 308

Query: 303 TLDLSDTGTSFILRRDSSLSGKSHNDDSPCLHSLQPSVSSKEDGESHISCQLNLTLASSS 362
            L+L+DTGT            +   +D+  + +LQ  ++S E+ +   SC LNLTLAS+ 
Sbjct: 309 ALELADTGT---------CPRRLLLNDNTRVETLQQRLTSSEETDGSFSCHLNLTLASAP 368

Query: 363 LRINDTACSVRMPQLGQNVRKFPDSKVNSNGKESDVRLFTKNINLDEGSTPVCPQETSNN 422
           L  + TA  +    L          ++N N  E+      KN    E +        S  
Sbjct: 369 LP-DKTASQIAKTTL-------KSQELNFNSIETSAS--EKNRGRQEIAV-----GGSQA 428

Query: 423 NHGPPAASIRANDVFWERLLTERPGCPESEEASSNYRANPFKEPDD 464
           N  PPA   R NDVFWE+ LTERPG  ++EEASS YR NP++E ++
Sbjct: 429 NAAPPA---RVNDVFWEQFLTERPGSSDNEEASSTYRGNPYEEQEE 447

BLAST of Cucsa.256700 vs. Swiss-Prot
Match: HSFA5_ORYSJ (Heat stress transcription factor A-5 OS=Oryza sativa subsp. japonica GN=HSFA5 PE=2 SV=1)

HSP 1 Score: 365.2 bits (936), Expect = 1.1e-99
Identity = 219/470 (46.60%), Postives = 289/470 (61.49%), Query Frame = 1

Query: 3   AAAAGGGTGGAGPAPFLIKTYDMVDDSSTDEIVSWT-SSKKSFVVWNPPEFARLLLPTFF 62
           A   G G GG GPAPFL+KTY+MVDD STD +VSW+ +S  SFVVWN PEFA  LLP +F
Sbjct: 7   ARGGGAGGGGGGPAPFLLKTYEMVDDPSTDAVVSWSDASDASFVVWNHPEFAARLLPAYF 66

Query: 63  KHSNFSSFIRQLNTYGFRKIDSEKWEFANEDFIKDQKHLLKNIHRRKPIHSHSNPQGSHI 122
           KHSNFSSFIRQLNTYGFRKID E+WEFANE FIK QKHLLKNIHRRKPIHSHS+P G+  
Sbjct: 67  KHSNFSSFIRQLNTYGFRKIDPERWEFANEYFIKGQKHLLKNIHRRKPIHSHSHPPGALP 126

Query: 123 DPERAAFEDEIERLAREKTTLETNISRFKQQKSTAKLQLQDLTVKVESMEKRQKNLLAFL 182
           D ERA FEDEIERL+REK+ L+ ++ + KQQ+S    Q++DL  +V  ME+RQ  ++AFL
Sbjct: 127 DNERAIFEDEIERLSREKSNLQADLWKSKQQQSGTMNQIEDLERRVLGMEQRQTKMIAFL 186

Query: 183 EKAVQNPSFVEHLARRVE-SMDFT-AFKKKRRLPSADLSQPVVE-NSFLDNHSS-SRSES 242
           ++A +NP FV  L +  E S  FT AF KKRRLP  D S    E  SF D+HSS S+ E+
Sbjct: 187 QQASKNPQFVNKLVKMAEASSIFTDAFNKKRRLPGLDYSIENTETTSFYDDHSSTSKQET 246

Query: 243 GNIFHQDFSQKLRLETSCA-SDINLISRSTQSSNEEGGSSQRQLSKFDTRAVQENIHFAV 302
           GN+ +Q FS KLRL    A ++ N+I+ STQSSNE+  S   +  + D    +E +    
Sbjct: 247 GNLLNQHFSDKLRLGLCPAMTESNIITLSTQSSNEDNRSPHGKHPECDMMG-RECLPLVP 306

Query: 303 ETLDLSDTGTSFILRRDSSLSGKSHNDDSPCLHSLQPSVSSKEDGESHISCQLNLTLASS 362
           + ++LSDTGTS    + S  +               P +S     E  ++C L+LTLAS 
Sbjct: 307 QMMELSDTGTSICPSKSSCFA---------------PPISD----EGLLTCHLSLTLASC 366

Query: 363 SLRI------NDTACSVRMPQLGQNVRKFPDSKVNSNGKESDVRLFTKNINLDEGSTPVC 422
           S+ +      N    ++  P          D  ++ +  ++  +  + +    + +TP  
Sbjct: 367 SMDVDKSQGLNANGTTIDNPTEAATATMEKDDTIDRSFDDNQKK--SADSRTADATTPRA 426

Query: 423 PQETSNNNHGPPAASIRANDVFWERLLTERPGCPESEEASSNYRANPFKE 461
               ++     PAA +  ND FWE+ LTERPGC E+EEASS  R +  +E
Sbjct: 427 DARVASEAPAAPAAVV--NDKFWEQFLTERPGCSETEEASSGLRTDTSRE 452

BLAST of Cucsa.256700 vs. Swiss-Prot
Match: HFA4A_ARATH (Heat stress transcription factor A-4a OS=Arabidopsis thaliana GN=HSFA4A PE=2 SV=1)

HSP 1 Score: 223.8 bits (569), Expect = 4.0e-57
Identity = 117/267 (43.82%), Postives = 168/267 (62.92%), Query Frame = 1

Query: 9   GTGGAGPAPFLIKTYDMVDDSSTDEIVSWTSSKKSFVVWNPPEFARLLLPTFFKHSNFSS 68
           G   +   PFL KTY+MVDDSS+D IVSW+ S KSF+VWNPPEF+R LLP FFKH+NFSS
Sbjct: 7   GVSSSSLPPFLTKTYEMVDDSSSDSIVSWSQSNKSFIVWNPPEFSRDLLPRFFKHNNFSS 66

Query: 69  FIRQLNTYGFRKIDSEKWEFANEDFIKDQKHLLKNIHRRKPIHSHSNPQ-----GSHIDP 128
           FIRQLNTYGFRK D E+WEFAN+DF++ Q HL+KNIHRRKP+HSHS P          D 
Sbjct: 67  FIRQLNTYGFRKADPEQWEFANDDFVRGQPHLMKNIHRRKPVHSHSLPNLQAQLNPLTDS 126

Query: 129 ERAAFEDEIERLAREKTTLETNISRFKQQKSTAKLQLQDLTVKVESMEKRQKNLLAFLEK 188
           ER    ++IERL +EK  L   + +  +++   ++Q+++L  +++ MEKRQK +++F+ +
Sbjct: 127 ERVRMNNQIERLTKEKEGLLEELHKQDEEREVFEMQVKELKERLQHMEKRQKTMVSFVSQ 186

Query: 189 AVQNPSFVEHLARRVESMDFTAFKKKRRLPSADL--SQPVVENSFLDNHSSSRSESGNIF 248
            ++ P    +L+  V   +    ++KRR P  +    +P++E +           +    
Sbjct: 187 VLEKPGLALNLSPCVPETN----ERKRRFPRIEFFPDEPMLEENKTCVVVREEGSTSPSS 246

Query: 249 HQDFSQKLRLETSCASDINLISRSTQS 269
           H    Q  +LE+S A   NL+S S +S
Sbjct: 247 HTREHQVEQLESSIAIWENLVSDSCES 269


HSP 2 Score: 37.4 bits (85), Expect = 5.3e-01
Identity = 38/159 (23.90%), Postives = 61/159 (38.36%), Query Frame = 1

Query: 292 IHFAVETLDLSDTGTSFILRRDSSLSGKSHNDDSPCLHSLQPSVSSKEDGESHISCQLNL 351
           I F  +   L +  T  ++R + S S  SH  +    H ++   SS    E+ +S     
Sbjct: 214 IEFFPDEPMLEENKTCVVVREEGSTSPSSHTRE----HQVEQLESSIAIWENLVSDSCES 273

Query: 352 TLASSSLRINDTACSVRMPQLG--QNVRKFPDSKVNSNGKESDVRLFTKNINLDEGSTPV 411
            L S S+   D   S   P+      ++   DS++ S      + +  +     E +T  
Sbjct: 274 MLQSRSMMTLDVDESSTFPESPPLSCIQLSVDSRLKSPPSPRIIDMNCEPDGSKEQNTVA 333

Query: 412 CPQETSNNNHGPPAASIRANDVFWERLLTERPGCPESEE 449
            P         PP     AND FW++  +E PG  E  E
Sbjct: 334 AP---------PPPPVAGANDGFWQQFFSENPGSTEQRE 359

BLAST of Cucsa.256700 vs. Swiss-Prot
Match: HFA4B_ORYSJ (Heat stress transcription factor A-4b OS=Oryza sativa subsp. japonica GN=HSFA4B PE=2 SV=1)

HSP 1 Score: 220.7 bits (561), Expect = 3.4e-56
Identity = 154/462 (33.33%), Postives = 234/462 (50.65%), Query Frame = 1

Query: 7   GGGTGGAGPAPFLIKTYDMVDDSSTDEIVSWTSSKKSFVVWNPPEFARLLLPTFFKHSNF 66
           GGG GG+ P PFL KTY+MVDD STD +V WT +  SFVV N PEF R LLP +FKH+NF
Sbjct: 3   GGGGGGSLP-PFLSKTYEMVDDPSTDAVVGWTPAGTSFVVANQPEFCRDLLPKYFKHNNF 62

Query: 67  SSFIRQLNTYGFRKIDSEKWEFANEDFIKDQKHLLKNIHRRKPIHSHSNPQ---GSHIDP 126
           SSF+RQLNTYGFRK+D E+WEFANEDFIK Q+H LKNIHRRKPI SHS+     G   D 
Sbjct: 63  SSFVRQLNTYGFRKVDPEQWEFANEDFIKGQRHRLKNIHRRKPIFSHSSHSQGAGPLTDN 122

Query: 127 ERAAFEDEIERLAREKTTLETNISRFKQQKSTAKLQLQDLTVKVESMEKRQKNLLAFLEK 186
           ER  +E+EIERL  +   L + +     +K   + ++Q L  K+  +E +Q++L++++ +
Sbjct: 123 ERKDYEEEIERLKSDNAALSSELQNNTLKKLNMEKRMQALEEKLFVVEDQQRSLISYVRE 182

Query: 187 AVQNPSFVEHLARRVESMDFTAFKKKRRLPSADLSQPVVENSFLDNHSSSRSESGNIFHQ 246
            V+ P F+    ++ +       +KKRRLP      P+                   FH+
Sbjct: 183 IVKAPGFLSSFVQQQDH-----HRKKRRLPI-----PIS------------------FHE 242

Query: 247 DFSQKLRLETSCASDINLISRSTQSSNEEGGSSQRQLSKFDTRAVQENIHFAVETLDLSD 306
           D + +      C    +      + S ++  SS   L  F   A +E  +       +  
Sbjct: 243 DANTQENQIMPCDLTNSPAQTFYRESFDKMESSLNSLENFLREASEEFGNDISYDDGVPG 302

Query: 307 TGTSFILRRDSSLSGKSHNDDSPCLHSLQPSVSSKEDGESHISCQLNLTLASSSLRINDT 366
             ++ +L   + L     +D        +   SS   G+SH S  +             T
Sbjct: 303 PSSTVVL---TELHSPGESDPRVSSPPTRMRTSSAGAGDSHSSRDV----------AEST 362

Query: 367 AC--SVRMPQLGQNVRKFPDSKVNSNGKESDVRLFTKNINLDEGSTPVCPQETSNNNHGP 426
           +C  S  +PQ+         S+V++  K S++ + ++    + G +   P E       P
Sbjct: 363 SCAESPPIPQM--------HSRVDTRAKVSEIDVNSEPAVTETGPSRDQPAEE------P 408

Query: 427 PAASIRANDVFWERLLTERPGCPESEEASSNYRANPFKEPDD 464
           PA +  AND FW++ LTE+PG  ++ + + + R +   + D+
Sbjct: 423 PAVTPGANDGFWQQFLTEQPGSSDAHQEAQSERRDGGNKVDE 408

BLAST of Cucsa.256700 vs. Swiss-Prot
Match: HFA4D_ORYSJ (Heat stress transcription factor A-4d OS=Oryza sativa subsp. japonica GN=HSFA4D PE=1 SV=1)

HSP 1 Score: 219.2 bits (557), Expect = 9.8e-56
Identity = 113/214 (52.80%), Postives = 145/214 (67.76%), Query Frame = 1

Query: 7   GGGTGGAGPAPFLIKTYDMVDDSSTDEIVSWTSSKKSFVVWNPPEFARLLLPTFFKHSNF 66
           GGG GG GP PFLIKTY+MV+D++T+ +VSW     SFVVWNP +F+R LLP +FKH+NF
Sbjct: 10  GGGGGGGGPPPFLIKTYEMVEDAATNHVVSWGPGGASFVVWNPLDFSRDLLPKYFKHNNF 69

Query: 67  SSFIRQLNTYGFRKIDSEKWEFANEDFIKDQKHLLKNIHRRKPIHSHS---NPQGSHIDP 126
           SSFIRQLNTYGFRKID E+WEFANEDFI+   HLLKNIHRRKP+HSHS      G   + 
Sbjct: 70  SSFIRQLNTYGFRKIDPERWEFANEDFIRGHTHLLKNIHRRKPVHSHSLQNQINGPLAES 129

Query: 127 ERAAFEDEIERLAREKTTLETNISRFKQQKSTAKLQLQDLTVKVESMEKRQKNLLAFLEK 186
           ER   E+EI RL  EK+ L  ++ R  QQ+     Q+Q +  ++ +ME+RQKN++A L +
Sbjct: 130 ERRELEEEINRLKYEKSILVADLQRQNQQQYVINWQMQAMEGRLVAMEQRQKNIVASLCE 189

Query: 187 AVQNPSFVEHLARRVESMDFTAFKKKRRLPSADL 218
            +Q        A     ++   F KKRR+P  DL
Sbjct: 190 MLQRRGG----AVSSSLLESDHFSKKRRVPKMDL 219

BLAST of Cucsa.256700 vs. TrEMBL
Match: A0A0A0L9B5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G129630 PE=3 SV=1)

HSP 1 Score: 935.6 bits (2417), Expect = 2.3e-269
Identity = 467/467 (100.00%), Postives = 467/467 (100.00%), Query Frame = 1

Query: 1   MEAAAAGGGTGGAGPAPFLIKTYDMVDDSSTDEIVSWTSSKKSFVVWNPPEFARLLLPTF 60
           MEAAAAGGGTGGAGPAPFLIKTYDMVDDSSTDEIVSWTSSKKSFVVWNPPEFARLLLPTF
Sbjct: 87  MEAAAAGGGTGGAGPAPFLIKTYDMVDDSSTDEIVSWTSSKKSFVVWNPPEFARLLLPTF 146

Query: 61  FKHSNFSSFIRQLNTYGFRKIDSEKWEFANEDFIKDQKHLLKNIHRRKPIHSHSNPQGSH 120
           FKHSNFSSFIRQLNTYGFRKIDSEKWEFANEDFIKDQKHLLKNIHRRKPIHSHSNPQGSH
Sbjct: 147 FKHSNFSSFIRQLNTYGFRKIDSEKWEFANEDFIKDQKHLLKNIHRRKPIHSHSNPQGSH 206

Query: 121 IDPERAAFEDEIERLAREKTTLETNISRFKQQKSTAKLQLQDLTVKVESMEKRQKNLLAF 180
           IDPERAAFEDEIERLAREKTTLETNISRFKQQKSTAKLQLQDLTVKVESMEKRQKNLLAF
Sbjct: 207 IDPERAAFEDEIERLAREKTTLETNISRFKQQKSTAKLQLQDLTVKVESMEKRQKNLLAF 266

Query: 181 LEKAVQNPSFVEHLARRVESMDFTAFKKKRRLPSADLSQPVVENSFLDNHSSSRSESGNI 240
           LEKAVQNPSFVEHLARRVESMDFTAFKKKRRLPSADLSQPVVENSFLDNHSSSRSESGNI
Sbjct: 267 LEKAVQNPSFVEHLARRVESMDFTAFKKKRRLPSADLSQPVVENSFLDNHSSSRSESGNI 326

Query: 241 FHQDFSQKLRLETSCASDINLISRSTQSSNEEGGSSQRQLSKFDTRAVQENIHFAVETLD 300
           FHQDFSQKLRLETSCASDINLISRSTQSSNEEGGSSQRQLSKFDTRAVQENIHFAVETLD
Sbjct: 327 FHQDFSQKLRLETSCASDINLISRSTQSSNEEGGSSQRQLSKFDTRAVQENIHFAVETLD 386

Query: 301 LSDTGTSFILRRDSSLSGKSHNDDSPCLHSLQPSVSSKEDGESHISCQLNLTLASSSLRI 360
           LSDTGTSFILRRDSSLSGKSHNDDSPCLHSLQPSVSSKEDGESHISCQLNLTLASSSLRI
Sbjct: 387 LSDTGTSFILRRDSSLSGKSHNDDSPCLHSLQPSVSSKEDGESHISCQLNLTLASSSLRI 446

Query: 361 NDTACSVRMPQLGQNVRKFPDSKVNSNGKESDVRLFTKNINLDEGSTPVCPQETSNNNHG 420
           NDTACSVRMPQLGQNVRKFPDSKVNSNGKESDVRLFTKNINLDEGSTPVCPQETSNNNHG
Sbjct: 447 NDTACSVRMPQLGQNVRKFPDSKVNSNGKESDVRLFTKNINLDEGSTPVCPQETSNNNHG 506

Query: 421 PPAASIRANDVFWERLLTERPGCPESEEASSNYRANPFKEPDDGTVH 468
           PPAASIRANDVFWERLLTERPGCPESEEASSNYRANPFKEPDDGTVH
Sbjct: 507 PPAASIRANDVFWERLLTERPGCPESEEASSNYRANPFKEPDDGTVH 553

BLAST of Cucsa.256700 vs. TrEMBL
Match: A0A061ES53_THECC (Winged-helix DNA-binding transcription factor family protein OS=Theobroma cacao GN=TCM_020222 PE=3 SV=1)

HSP 1 Score: 596.3 bits (1536), Expect = 3.3e-167
Identity = 300/465 (64.52%), Postives = 364/465 (78.28%), Query Frame = 1

Query: 4   AAAGGGTGGAGPAPFLIKTYDMVDDSSTDEIVSWTSSKKSFVVWNPPEFARLLLPTFFKH 63
           +A+GGG GG GPAPFL+KTYDMVDDSSTD+IVSW+S+KKSFVVWNPPEFARLLLPT+FKH
Sbjct: 7   SASGGGGGGGGPAPFLLKTYDMVDDSSTDDIVSWSSNKKSFVVWNPPEFARLLLPTYFKH 66

Query: 64  SNFSSFIRQLNTYGFRKIDSEKWEFANEDFIKDQKHLLKNIHRRKPIHSHSNPQGSHIDP 123
           +NFSSFIRQLNTYGFRKID E+WEFANEDF+KDQKHLLKNIHRRKPIHSHS+PQGS IDP
Sbjct: 67  NNFSSFIRQLNTYGFRKIDPERWEFANEDFVKDQKHLLKNIHRRKPIHSHSHPQGSLIDP 126

Query: 124 ERAAFEDEIERLAREKTTLETNISRFKQQKSTAKLQLQDLTVKVESMEKRQKNLLAFLEK 183
           ERA FE+EIE+L+REK  LE N+ RF+Q++S AK QL++L  + + ME+RQ  L  FLEK
Sbjct: 127 ERAGFEEEIEKLSREKAALEANVLRFRQERSAAKHQLEELAQRADQMERRQDTLFNFLEK 186

Query: 184 AVQNPSFVEHLARRVESMDFTAFKKKRRLPSADLSQPVVENSFLDNHSSSRSESGNIFHQ 243
           A Q+P FVEHL R++ESMD TA+ KKRRLP  D  +PV ENS LDN+SSSRSE GNIFHQ
Sbjct: 187 AAQDPIFVEHLVRKIESMDVTAYNKKRRLPQVDQIKPVGENSLLDNNSSSRSEFGNIFHQ 246

Query: 244 DFSQKLRLETSCA-SDINLISRSTQSSNEEGGSSQRQLSKFDTRAVQ---ENIHFAVETL 303
           DFS KLRLE S A SDINL+S STQSSNE+G S QR++S+ + +  Q   E + F  ETL
Sbjct: 247 DFSNKLRLELSPAVSDINLVSHSTQSSNEDGVSPQRRISEGEPKDAQTRPEGLLFTPETL 306

Query: 304 DLSDTGTSFILRRDSSLSGKSHNDDSPCLHSLQPSVSSKEDGESHISCQLNLTLASSSLR 363
           DLSDTGTSF  + DSS + +   ++SP +HSLQ  ++S E+ +SHISCQLNLTLASSSL 
Sbjct: 307 DLSDTGTSFTFKMDSSFTQRVPMNESPPVHSLQQRLNSNEEPDSHISCQLNLTLASSSLH 366

Query: 364 INDTACSVRMPQLGQNVRKFPDSKVNSNGKESDVRLFTKNINL-DEGSTPVCPQETSNNN 423
           +N +    RM Q GQ   K P+S+ N+N K+SD R F  N N+ D+ +    P E  N N
Sbjct: 367 VNRSPSLTRMSQQGQETGKGPESRSNANTKDSDTRAFENNRNMVDDEAALSSPIEAPNIN 426

Query: 424 HGPPAASIRANDVFWERLLTERPGCPESEEASSNYRANPFKEPDD 464
             P A  +R ND+FWE+ LTERPG  E+EEASS+YRANP++E +D
Sbjct: 427 QEPAAPPVRVNDIFWEQFLTERPGSSENEEASSSYRANPYEEQED 471

BLAST of Cucsa.256700 vs. TrEMBL
Match: B9S9Y4_RICCO (DNA binding protein, putative OS=Ricinus communis GN=RCOM_0523380 PE=3 SV=1)

HSP 1 Score: 589.7 bits (1519), Expect = 3.1e-165
Identity = 299/467 (64.03%), Postives = 364/467 (77.94%), Query Frame = 1

Query: 3   AAAAGGGTGGAGPAPFLIKTYDMVDDSSTDEIVSWTSSKKSFVVWNPPEFARLLLPTFFK 62
           +AAA  G GG GPAPFL+KTYDMVDD++TD+IVSW+S+K SFVVWNPPEFARLLLPT+FK
Sbjct: 6   SAAAAAGGGGGGPAPFLLKTYDMVDDTATDDIVSWSSAKNSFVVWNPPEFARLLLPTYFK 65

Query: 63  HSNFSSFIRQLNTYGFRKIDSEKWEFANEDFIKDQKHLLKNIHRRKPIHSHSNPQGSHID 122
           H+NFSSFIRQLNTYGFRKID EKWEFANEDF+KDQKHLLKNIHRRKPIHSHSNP GS +D
Sbjct: 66  HNNFSSFIRQLNTYGFRKIDPEKWEFANEDFVKDQKHLLKNIHRRKPIHSHSNPPGSAVD 125

Query: 123 PERAAFEDEIERLAREKTTLETNISRFKQQKSTAKLQLQDLTVKVESMEKRQKNLLAFLE 182
           PERAAF++EI+RL  EK TLE NI R+K+Q+S  KLQL+DL  KV+SM +RQ+ LLAFLE
Sbjct: 126 PERAAFDEEIDRLTHEKATLEANIVRYKKQQSAEKLQLEDLMQKVDSMGQRQEKLLAFLE 185

Query: 183 KAVQNPSFVEHLARRVESMDFTAFKKKRRLPSADLSQPVVENSFLDNHSSSRSESGNIFH 242
           KAVQNP+FVE+LA+++ESMDF+A+ KKRRLP  D S+ + ENSF+ NHS +RSE GN+ H
Sbjct: 186 KAVQNPTFVENLAQKIESMDFSAYSKKRRLPQVDHSKSIAENSFVGNHSITRSEFGNVIH 245

Query: 243 QDFSQKLRLETSCA-SDINLISRSTQSSNEEGGSSQRQLSKFDTRAVQEN---IHFAVE- 302
           QDFS KLRLE S A SDINL+S STQSSNE+GGS QR++S  D +        + FA E 
Sbjct: 246 QDFSNKLRLELSPAVSDINLVSDSTQSSNEDGGSPQRKISGGDPKDAHPRTPCLLFAAEA 305

Query: 303 TLDLSDTGTSFILRRDSSLSGKSHNDDSPCLHSLQPSVSSKEDGESHISCQLNLTLASSS 362
           T +LSDTGTS+  + D +   +   +++P LH LQ ++SS E+ + HISC LNLTLASS 
Sbjct: 306 TFELSDTGTSYTYKMDPNFPRRVTANETPELHLLQQNLSSNEEVDGHISCHLNLTLASSP 365

Query: 363 LRINDTACSVRMPQLGQNVRKFPDSKVNSNGKESDVRLFTKNINLDEGSTPV-CPQETSN 422
           L+IN T  S RMP+LGQ +    +SK N NGKESD R+  K     EG T +   +E  N
Sbjct: 366 LQINRTPYSARMPELGQEICTSSESKFNENGKESDARVTLKERYAGEGKTILSSSKEAPN 425

Query: 423 NNHGPPAASIRANDVFWERLLTERPGCPESEEASSNYRANPFKEPDD 464
           NN GP AA  R ND+FWE+ LTERPG  ++EEASSNYRANP++E +D
Sbjct: 426 NNQGPAAAPPRVNDIFWEQFLTERPGSSDNEEASSNYRANPYEEQED 472

BLAST of Cucsa.256700 vs. TrEMBL
Match: B9GFJ0_POPTR (Heat Stress Transcription Factor family protein OS=Populus trichocarpa GN=POPTR_0001s32810g PE=3 SV=2)

HSP 1 Score: 583.9 bits (1504), Expect = 1.7e-163
Identity = 291/463 (62.85%), Postives = 365/463 (78.83%), Query Frame = 1

Query: 3   AAAAGGGTGGAGPAPFLIKTYDMVDDSSTDEIVSWTSSKKSFVVWNPPEFARLLLPTFFK 62
           A + GGG GG GPAPFL+KTYDMVDDSSTDEIVSW+S+K SFVVWNPPEFARLLLPTFFK
Sbjct: 8   ATSGGGGGGGGGPAPFLVKTYDMVDDSSTDEIVSWSSNKNSFVVWNPPEFARLLLPTFFK 67

Query: 63  HSNFSSFIRQLNTYGFRKIDSEKWEFANEDFIKDQKHLLKNIHRRKPIHSHSNPQGSHID 122
           H+NFSSFIRQLNTYGFRKID E+WEFANEDF+KDQKHLLKNI+RRKPIHSHS PQGS +D
Sbjct: 68  HNNFSSFIRQLNTYGFRKIDPERWEFANEDFVKDQKHLLKNIYRRKPIHSHSQPQGSLVD 127

Query: 123 PERAAFEDEIERLAREKTTLETNISRFKQQKSTAKLQLQDLTVKVESMEKRQKNLLAFLE 182
           PERAA+E+EIE+LAR+K  L+ +I  F+QQ+S+AKLQ++DLT K+++M++RQ+ LL+FLE
Sbjct: 128 PERAAYEEEIEKLARDKAKLKASILGFEQQRSSAKLQVEDLTQKIDTMQQRQEKLLSFLE 187

Query: 183 KAVQNPSFVEHLARRVESMDFTAFKKKRRLPSADLSQPVVENSFLDNHSSSRSESGNIFH 242
           KAVQNP+FVEHLAR++E+MDF+A+ KKRRLP  D   P+ ENS ++NHSSSR ES N+ H
Sbjct: 188 KAVQNPTFVEHLARKIEAMDFSAYSKKRRLPQVDHPMPIAENSLVENHSSSRPES-NVIH 247

Query: 243 QDFSQKLRLETSCA-SDINLISRSTQSSNEEGGSSQRQLSK---FDTRAVQENIHFAVET 302
           QDF  KLRLE S A SDINL+S STQSSNE+GGS QR++S+    D       +  A ET
Sbjct: 248 QDFPDKLRLELSPAVSDINLVSHSTQSSNEDGGSPQRKISEGNPKDALTRTSGLLLAPET 307

Query: 303 LDLSDTGTSFILRRDSSLSGKSHNDDSPCLHSLQPSVSSKEDGESHISCQLNLTLASSSL 362
           L+LSDTG S+  + + ++      + SP LHSLQ +++S E+ + HISCQLNL+LASS L
Sbjct: 308 LELSDTGASYAFKVNPAVPRDIPANGSPALHSLQSNLTSNEEVDGHISCQLNLSLASSPL 367

Query: 363 RINDTACSVRMPQLGQNVRKFPDSKVNSNGKESDVRLFTKNINL-DEGSTPVCPQETSNN 422
           ++N      R+PQLGQ + K P+S+ N + K+SD+R+   N+NL +E       QET NN
Sbjct: 368 QVNKNPYLTRIPQLGQEIGKSPESRFNESNKDSDIRVSQNNMNLGNEVRALSNSQETPNN 427

Query: 423 NHGPPAASIRANDVFWERLLTERPGCPESEEASSNYRANPFKE 461
           N  P +A +R NDVFWE+ LTERPG  ++EEASSNYRANP+ E
Sbjct: 428 NQAPASAPVRVNDVFWEQFLTERPGYSDNEEASSNYRANPYDE 469

BLAST of Cucsa.256700 vs. TrEMBL
Match: A0A067L5F4_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_01081 PE=3 SV=1)

HSP 1 Score: 577.8 bits (1488), Expect = 1.2e-161
Identity = 296/466 (63.52%), Postives = 357/466 (76.61%), Query Frame = 1

Query: 3   AAAAGGGTGGAGPAPFLIKTYDMVDDSSTDEIVSWTSSKKSFVVWNPPEFARLLLPTFFK 62
           +   GGG GG GPAPFL+KTYDMVDDSSTDEIVSW+SSK SFVVWNPPEFAR+LLPT+FK
Sbjct: 11  SGCVGGGGGGGGPAPFLLKTYDMVDDSSTDEIVSWSSSKNSFVVWNPPEFARVLLPTYFK 70

Query: 63  HSNFSSFIRQLNTYGFRKIDSEKWEFANEDFIKDQKHLLKNIHRRKPIHSHSNPQGSHID 122
           H+NFSSFIRQLNTYGFRKID EKWEFANEDF+KDQKHLLKNIHRRKPIHSHS+P GS +D
Sbjct: 71  HNNFSSFIRQLNTYGFRKIDPEKWEFANEDFVKDQKHLLKNIHRRKPIHSHSHPHGSLVD 130

Query: 123 PERAAFEDEIERLAREKTTLETNISRFKQQKSTAKLQLQDLTVKVESMEKRQKNLLAFLE 182
           PERAAF+ EI+RLAREK TLE ++   K Q+S  KLQL+DLT +V SME+RQ+ LLA LE
Sbjct: 131 PERAAFDVEIDRLAREKATLEESVVGSKHQRSAEKLQLEDLTQRVNSMEQRQEKLLALLE 190

Query: 183 KAVQNPSFVEHLARRVESMDFTAFKKKRRLPSADLSQPVVENSFLDNHSSSRSESGNIFH 242
           KAVQNP+F+EHLAR+++SMDF+A+ KKRRLP  D S+   ENSF+DN S+SRSE GN+ H
Sbjct: 191 KAVQNPTFIEHLARKIDSMDFSAYSKKRRLPQVDHSKSNAENSFVDNQSNSRSEFGNVIH 250

Query: 243 QDFSQKLRLETSCA-SDINLISRSTQSSNEEGGSSQRQLSKFDTRAVQEN---IHFAVET 302
           QDFS KLRLE S A SDINL+S STQSSNE+ GS QR+ S  D +        + FA ET
Sbjct: 251 QDFSNKLRLELSPAVSDINLVSNSTQSSNEDMGSPQRKASGGDPKDTPPRTPCLLFASET 310

Query: 303 LDLSDTGTSFILRRDSSLSGKSHNDDSPCLHSLQPSVSSKEDGESHISCQLNLTLASSSL 362
            DLSDTGTS+  + D +   K   + +  LHSLQP++ S E+ + HISC LNLTLASS L
Sbjct: 311 FDLSDTGTSYSYKVDPAFPRKLTTNKTLELHSLQPNLISNEEADGHISCHLNLTLASSPL 370

Query: 363 RINDTACSVRMPQLGQNVRKFPDSKVNSNGKESDVRLFTKNINLDEGSTPV-CPQETSNN 422
           + N +  S RM +L Q   K P+SK+N +GKES++ + +K+     G T +   +E  NN
Sbjct: 371 QANRSPYSARMAELHQEFCKSPESKLNDDGKESNMLVTSKDRTGGNGDTTLSSSKEAPNN 430

Query: 423 NHGPPAASIRANDVFWERLLTERPGCPESEEASSNYRANPFKEPDD 464
           N GP AA  R NDVFWE+ LTERPG  E+EEASSNYRANP+ + +D
Sbjct: 431 NQGPAAAPPRVNDVFWEQFLTERPGSSENEEASSNYRANPYDDKED 476

BLAST of Cucsa.256700 vs. TAIR10
Match: AT4G13980.1 (AT4G13980.1 winged-helix DNA-binding transcription factor family protein)

HSP 1 Score: 438.0 bits (1125), Expect = 7.5e-123
Identity = 240/466 (51.50%), Postives = 314/466 (67.38%), Query Frame = 1

Query: 3   AAAAGGGTGGAGPAPFLIKTYDMVDDSSTDEIVSWTSSKKSFVVWNPPEFARLLLPTFFK 62
           +A+  GG G  GPAPFL+KTY+MVDDSSTD+IVSW+++  SF+VWN  EF+RLLLPT+FK
Sbjct: 9   SASVSGGEGAGGPAPFLVKTYEMVDDSSTDQIVSWSANNNSFIVWNHAEFSRLLLPTYFK 68

Query: 63  HSNFSSFIRQLNTYGFRKIDSEKWEFANEDFIKDQKHLLKNIHRRKPIHSHSNPQGSHID 122
           H+NFSSFIRQLNTYGFRKID E+WEF N+DFIKDQKHLLKNIHRRKPIHSHS+P  S  D
Sbjct: 69  HNNFSSFIRQLNTYGFRKIDPERWEFLNDDFIKDQKHLLKNIHRRKPIHSHSHPPASSTD 128

Query: 123 PERAAFEDEIERLAREKTTLETNISRFKQQKSTAKLQLQDLTVKVESMEKRQKNLLAFLE 182
            ERA  ++++++L+REK  +E  + +FKQQK  AK Q +++T  V+ ME RQK LL FLE
Sbjct: 129 QERAVLQEQMDKLSREKAAIEAKLLKFKQQKVVAKHQFEEMTEHVDDMENRQKKLLNFLE 188

Query: 183 KAVQNPSFVEHLARRVESMDFTAFKKKRRLPSADLSQPVVENSFLDNHS-SSRSESGNIF 242
            A++NP+FV++  ++VE +D +A+ KKRRLP  + S+P  E+S LDN S SSR ESGNIF
Sbjct: 189 TAIRNPTFVKNFGKKVEQLDISAYNKKRRLPEVEQSKPPSEDSHLDNSSGSSRRESGNIF 248

Query: 243 HQDFSQKLRLETSCA-SDINLISRSTQSSNEEGGSSQRQLSKFD---TRAVQENIHFAVE 302
           HQ+FS KLRLE S A SD+N++S S QSSNEEG S +  LS  D   T   +E + FA E
Sbjct: 249 HQNFSNKLRLELSPADSDMNMVSHSIQSSNEEGASPKGILSGGDPNTTLTKREGLPFAPE 308

Query: 303 TLDLSDTGTSFILRRDSSLSGKSHNDDSPCLHSLQPSVSSKEDGESHISCQLNLTLASSS 362
            L+L+DTGT            +   +D+  + +LQ  ++S E+ +   SC LNLTLAS+ 
Sbjct: 309 ALELADTGT---------CPRRLLLNDNTRVETLQQRLTSSEETDGSFSCHLNLTLASAP 368

Query: 363 LRINDTACSVRMPQLGQNVRKFPDSKVNSNGKESDVRLFTKNINLDEGSTPVCPQETSNN 422
           L  + TA  +    L          ++N N  E+      KN    E +        S  
Sbjct: 369 LP-DKTASQIAKTTL-------KSQELNFNSIETSAS--EKNRGRQEIAV-----GGSQA 428

Query: 423 NHGPPAASIRANDVFWERLLTERPGCPESEEASSNYRANPFKEPDD 464
           N  PPA   R NDVFWE+ LTERPG  ++EEASS YR NP++E ++
Sbjct: 429 NAAPPA---RVNDVFWEQFLTERPGSSDNEEASSTYRGNPYEEQEE 447

BLAST of Cucsa.256700 vs. TAIR10
Match: AT4G18880.1 (AT4G18880.1 heat shock transcription factor A4A)

HSP 1 Score: 223.8 bits (569), Expect = 2.2e-58
Identity = 117/267 (43.82%), Postives = 168/267 (62.92%), Query Frame = 1

Query: 9   GTGGAGPAPFLIKTYDMVDDSSTDEIVSWTSSKKSFVVWNPPEFARLLLPTFFKHSNFSS 68
           G   +   PFL KTY+MVDDSS+D IVSW+ S KSF+VWNPPEF+R LLP FFKH+NFSS
Sbjct: 7   GVSSSSLPPFLTKTYEMVDDSSSDSIVSWSQSNKSFIVWNPPEFSRDLLPRFFKHNNFSS 66

Query: 69  FIRQLNTYGFRKIDSEKWEFANEDFIKDQKHLLKNIHRRKPIHSHSNPQ-----GSHIDP 128
           FIRQLNTYGFRK D E+WEFAN+DF++ Q HL+KNIHRRKP+HSHS P          D 
Sbjct: 67  FIRQLNTYGFRKADPEQWEFANDDFVRGQPHLMKNIHRRKPVHSHSLPNLQAQLNPLTDS 126

Query: 129 ERAAFEDEIERLAREKTTLETNISRFKQQKSTAKLQLQDLTVKVESMEKRQKNLLAFLEK 188
           ER    ++IERL +EK  L   + +  +++   ++Q+++L  +++ MEKRQK +++F+ +
Sbjct: 127 ERVRMNNQIERLTKEKEGLLEELHKQDEEREVFEMQVKELKERLQHMEKRQKTMVSFVSQ 186

Query: 189 AVQNPSFVEHLARRVESMDFTAFKKKRRLPSADL--SQPVVENSFLDNHSSSRSESGNIF 248
            ++ P    +L+  V   +    ++KRR P  +    +P++E +           +    
Sbjct: 187 VLEKPGLALNLSPCVPETN----ERKRRFPRIEFFPDEPMLEENKTCVVVREEGSTSPSS 246

Query: 249 HQDFSQKLRLETSCASDINLISRSTQS 269
           H    Q  +LE+S A   NL+S S +S
Sbjct: 247 HTREHQVEQLESSIAIWENLVSDSCES 269


HSP 2 Score: 37.4 bits (85), Expect = 3.0e-02
Identity = 38/159 (23.90%), Postives = 61/159 (38.36%), Query Frame = 1

Query: 292 IHFAVETLDLSDTGTSFILRRDSSLSGKSHNDDSPCLHSLQPSVSSKEDGESHISCQLNL 351
           I F  +   L +  T  ++R + S S  SH  +    H ++   SS    E+ +S     
Sbjct: 214 IEFFPDEPMLEENKTCVVVREEGSTSPSSHTRE----HQVEQLESSIAIWENLVSDSCES 273

Query: 352 TLASSSLRINDTACSVRMPQLG--QNVRKFPDSKVNSNGKESDVRLFTKNINLDEGSTPV 411
            L S S+   D   S   P+      ++   DS++ S      + +  +     E +T  
Sbjct: 274 MLQSRSMMTLDVDESSTFPESPPLSCIQLSVDSRLKSPPSPRIIDMNCEPDGSKEQNTVA 333

Query: 412 CPQETSNNNHGPPAASIRANDVFWERLLTERPGCPESEE 449
            P         PP     AND FW++  +E PG  E  E
Sbjct: 334 AP---------PPPPVAGANDGFWQQFFSENPGSTEQRE 359

BLAST of Cucsa.256700 vs. TAIR10
Match: AT1G32330.1 (AT1G32330.1 heat shock transcription factor A1D)

HSP 1 Score: 209.5 bits (532), Expect = 4.4e-54
Identity = 115/291 (39.52%), Postives = 169/291 (58.08%), Query Frame = 1

Query: 15  PAPFLIKTYDMVDDSSTDEIVSWTSSKKSFVVWNPPEFARLLLPTFFKHSNFSSFIRQLN 74
           P PFL KTYDMVDD +TD IVSW+++  SF+VW PPEFAR LLP  FKH+NFSSF+RQLN
Sbjct: 35  PPPFLSKTYDMVDDHNTDSIVSWSANNNSFIVWKPPEFARDLLPKNFKHNNFSSFVRQLN 94

Query: 75  TYGFRKIDSEKWEFANEDFIKDQKHLLKNIHRRKPIH---------SHSNPQGSH----I 134
           TYGFRK+D ++WEFANE F++ QKHLL++I RRKP H          HSN Q S     +
Sbjct: 95  TYGFRKVDPDRWEFANEGFLRGQKHLLQSITRRKPAHGQGQGHQRSQHSNGQNSSVSACV 154

Query: 135 DPERAAFEDEIERLAREKTTLETNISRFKQQKSTAKLQLQDLTVKVESMEKRQKNLLAFL 194
           +  +   E+E+ERL R+K  L   + R +QQ+ +   QLQ +  +++ ME RQ+ L++FL
Sbjct: 155 EVGKFGLEEEVERLKRDKNVLMQELVRLRQQQQSTDNQLQTMVQRLQGMENRQQQLMSFL 214

Query: 195 EKAVQNPSFVEHLARRVESMDFTAFKKKRRLPSADLSQPVVENSFLDNHSSSRSESGNIF 254
            KAVQ+P F+    ++    +    +  RR+      +    +  + N+ S+  +   + 
Sbjct: 215 AKAVQSPHFLSQFLQQQNQQN----ESNRRISDTSKKRRFKRDGIVRNNDSATPDGQIVK 274

Query: 255 HQD---------FSQKLRLETSCASDINLISRSTQSSNE--EGGSSQRQLS 282
           +Q          F Q +++E     D   +  +  S+ E  E  +S  Q+S
Sbjct: 275 YQPPMHEQAKAMFKQLMKMEPYKTGDDGFLLGNGTSTTEGTEMETSSNQVS 321

BLAST of Cucsa.256700 vs. TAIR10
Match: AT5G16820.1 (AT5G16820.1 heat shock factor 3)

HSP 1 Score: 208.0 bits (528), Expect = 1.3e-53
Identity = 102/209 (48.80%), Postives = 143/209 (68.42%), Query Frame = 1

Query: 17  PFLIKTYDMVDDSSTDEIVSWTSSKKSFVVWNPPEFARLLLPTFFKHSNFSSFIRQLNTY 76
           PFL KTYDMVDD  T+E+VSW+S   SFVVW+ PEF+++LLP +FKH+NFSSF+RQLNTY
Sbjct: 27  PFLSKTYDMVDDPLTNEVVSWSSGNNSFVVWSAPEFSKVLLPKYFKHNNFSSFVRQLNTY 86

Query: 77  GFRKIDSEKWEFANEDFIKDQKHLLKNIHRRKPIHSHSNPQ---------GSHIDPERAA 136
           GFRK+D ++WEFANE F++ +K LLK+I RRKP H   N Q         G+ ++  +  
Sbjct: 87  GFRKVDPDRWEFANEGFLRGRKQLLKSIVRRKPSHVQQNQQQTQVQSSSVGACVEVGKFG 146

Query: 137 FEDEIERLAREKTTLETNISRFKQQKSTAKLQLQDLTVKVESMEKRQKNLLAFLEKAVQN 196
            E+E+ERL R+K  L   + R +QQ+   + QLQ++  KV+ ME+RQ+ +++FL KAVQ+
Sbjct: 147 IEEEVERLKRDKNVLMQELVRLRQQQQATENQLQNVGQKVQVMEQRQQQMMSFLAKAVQS 206

Query: 197 PSFVEHLARRVE---SMDFTAFKKKRRLP 214
           P F+  L ++     +       KKRRLP
Sbjct: 207 PGFLNQLVQQNNNDGNRQIPGSNKKRRLP 235

BLAST of Cucsa.256700 vs. TAIR10
Match: AT4G17750.1 (AT4G17750.1 heat shock factor 1)

HSP 1 Score: 207.2 bits (526), Expect = 2.2e-53
Identity = 111/249 (44.58%), Postives = 156/249 (62.65%), Query Frame = 1

Query: 15  PAPFLIKTYDMVDDSSTDEIVSWTSSKKSFVVWNPPEFARLLLPTFFKHSNFSSFIRQLN 74
           P PFL KTYDMV+D +TD IVSW+ +  SF+VW+PPEF+R LLP +FKH+NFSSF+RQLN
Sbjct: 50  PPPFLSKTYDMVEDPATDAIVSWSPTNNSFIVWDPPEFSRDLLPKYFKHNNFSSFVRQLN 109

Query: 75  TYGFRKIDSEKWEFANEDFIKDQKHLLKNIHRRKPIHSH----SNPQ------------- 134
           TYGFRK+D ++WEFANE F++ QKHLLK I RRK +  H    SNPQ             
Sbjct: 110 TYGFRKVDPDRWEFANEGFLRGQKHLLKKISRRKSVQGHGSSSSNPQSQQLSQGQGSMAA 169

Query: 135 -GSHIDPERAAFEDEIERLAREKTTLETNISRFKQQKSTAKLQLQDLTVKVESMEKRQKN 194
             S ++  +   E+E+E+L R+K  L   + + +QQ+ T   +LQ L   ++ ME+RQ+ 
Sbjct: 170 LSSCVEVGKFGLEEEVEQLKRDKNVLMQELVKLRQQQQTTDNKLQVLVKHLQVMEQRQQQ 229

Query: 195 LLAFLEKAVQNPSFVEHLARRV--ESMDFTAFKKKRRLPSADLSQPVVENSFLDNHSSSR 244
           +++FL KAVQNP+F+    ++    +M  T   KKRRL   D +     NS   +HS   
Sbjct: 230 IMSFLAKAVQNPTFLSQFIQKQTDSNMHVTEANKKRRL-REDSTAATESNS--HSHSLEA 289

BLAST of Cucsa.256700 vs. NCBI nr
Match: gi|449433171|ref|XP_004134371.1| (PREDICTED: heat stress transcription factor A-5 [Cucumis sativus])

HSP 1 Score: 935.6 bits (2417), Expect = 3.3e-269
Identity = 467/467 (100.00%), Postives = 467/467 (100.00%), Query Frame = 1

Query: 1   MEAAAAGGGTGGAGPAPFLIKTYDMVDDSSTDEIVSWTSSKKSFVVWNPPEFARLLLPTF 60
           MEAAAAGGGTGGAGPAPFLIKTYDMVDDSSTDEIVSWTSSKKSFVVWNPPEFARLLLPTF
Sbjct: 1   MEAAAAGGGTGGAGPAPFLIKTYDMVDDSSTDEIVSWTSSKKSFVVWNPPEFARLLLPTF 60

Query: 61  FKHSNFSSFIRQLNTYGFRKIDSEKWEFANEDFIKDQKHLLKNIHRRKPIHSHSNPQGSH 120
           FKHSNFSSFIRQLNTYGFRKIDSEKWEFANEDFIKDQKHLLKNIHRRKPIHSHSNPQGSH
Sbjct: 61  FKHSNFSSFIRQLNTYGFRKIDSEKWEFANEDFIKDQKHLLKNIHRRKPIHSHSNPQGSH 120

Query: 121 IDPERAAFEDEIERLAREKTTLETNISRFKQQKSTAKLQLQDLTVKVESMEKRQKNLLAF 180
           IDPERAAFEDEIERLAREKTTLETNISRFKQQKSTAKLQLQDLTVKVESMEKRQKNLLAF
Sbjct: 121 IDPERAAFEDEIERLAREKTTLETNISRFKQQKSTAKLQLQDLTVKVESMEKRQKNLLAF 180

Query: 181 LEKAVQNPSFVEHLARRVESMDFTAFKKKRRLPSADLSQPVVENSFLDNHSSSRSESGNI 240
           LEKAVQNPSFVEHLARRVESMDFTAFKKKRRLPSADLSQPVVENSFLDNHSSSRSESGNI
Sbjct: 181 LEKAVQNPSFVEHLARRVESMDFTAFKKKRRLPSADLSQPVVENSFLDNHSSSRSESGNI 240

Query: 241 FHQDFSQKLRLETSCASDINLISRSTQSSNEEGGSSQRQLSKFDTRAVQENIHFAVETLD 300
           FHQDFSQKLRLETSCASDINLISRSTQSSNEEGGSSQRQLSKFDTRAVQENIHFAVETLD
Sbjct: 241 FHQDFSQKLRLETSCASDINLISRSTQSSNEEGGSSQRQLSKFDTRAVQENIHFAVETLD 300

Query: 301 LSDTGTSFILRRDSSLSGKSHNDDSPCLHSLQPSVSSKEDGESHISCQLNLTLASSSLRI 360
           LSDTGTSFILRRDSSLSGKSHNDDSPCLHSLQPSVSSKEDGESHISCQLNLTLASSSLRI
Sbjct: 301 LSDTGTSFILRRDSSLSGKSHNDDSPCLHSLQPSVSSKEDGESHISCQLNLTLASSSLRI 360

Query: 361 NDTACSVRMPQLGQNVRKFPDSKVNSNGKESDVRLFTKNINLDEGSTPVCPQETSNNNHG 420
           NDTACSVRMPQLGQNVRKFPDSKVNSNGKESDVRLFTKNINLDEGSTPVCPQETSNNNHG
Sbjct: 361 NDTACSVRMPQLGQNVRKFPDSKVNSNGKESDVRLFTKNINLDEGSTPVCPQETSNNNHG 420

Query: 421 PPAASIRANDVFWERLLTERPGCPESEEASSNYRANPFKEPDDGTVH 468
           PPAASIRANDVFWERLLTERPGCPESEEASSNYRANPFKEPDDGTVH
Sbjct: 421 PPAASIRANDVFWERLLTERPGCPESEEASSNYRANPFKEPDDGTVH 467

BLAST of Cucsa.256700 vs. NCBI nr
Match: gi|700201573|gb|KGN56706.1| (hypothetical protein Csa_3G129630 [Cucumis sativus])

HSP 1 Score: 935.6 bits (2417), Expect = 3.3e-269
Identity = 467/467 (100.00%), Postives = 467/467 (100.00%), Query Frame = 1

Query: 1   MEAAAAGGGTGGAGPAPFLIKTYDMVDDSSTDEIVSWTSSKKSFVVWNPPEFARLLLPTF 60
           MEAAAAGGGTGGAGPAPFLIKTYDMVDDSSTDEIVSWTSSKKSFVVWNPPEFARLLLPTF
Sbjct: 87  MEAAAAGGGTGGAGPAPFLIKTYDMVDDSSTDEIVSWTSSKKSFVVWNPPEFARLLLPTF 146

Query: 61  FKHSNFSSFIRQLNTYGFRKIDSEKWEFANEDFIKDQKHLLKNIHRRKPIHSHSNPQGSH 120
           FKHSNFSSFIRQLNTYGFRKIDSEKWEFANEDFIKDQKHLLKNIHRRKPIHSHSNPQGSH
Sbjct: 147 FKHSNFSSFIRQLNTYGFRKIDSEKWEFANEDFIKDQKHLLKNIHRRKPIHSHSNPQGSH 206

Query: 121 IDPERAAFEDEIERLAREKTTLETNISRFKQQKSTAKLQLQDLTVKVESMEKRQKNLLAF 180
           IDPERAAFEDEIERLAREKTTLETNISRFKQQKSTAKLQLQDLTVKVESMEKRQKNLLAF
Sbjct: 207 IDPERAAFEDEIERLAREKTTLETNISRFKQQKSTAKLQLQDLTVKVESMEKRQKNLLAF 266

Query: 181 LEKAVQNPSFVEHLARRVESMDFTAFKKKRRLPSADLSQPVVENSFLDNHSSSRSESGNI 240
           LEKAVQNPSFVEHLARRVESMDFTAFKKKRRLPSADLSQPVVENSFLDNHSSSRSESGNI
Sbjct: 267 LEKAVQNPSFVEHLARRVESMDFTAFKKKRRLPSADLSQPVVENSFLDNHSSSRSESGNI 326

Query: 241 FHQDFSQKLRLETSCASDINLISRSTQSSNEEGGSSQRQLSKFDTRAVQENIHFAVETLD 300
           FHQDFSQKLRLETSCASDINLISRSTQSSNEEGGSSQRQLSKFDTRAVQENIHFAVETLD
Sbjct: 327 FHQDFSQKLRLETSCASDINLISRSTQSSNEEGGSSQRQLSKFDTRAVQENIHFAVETLD 386

Query: 301 LSDTGTSFILRRDSSLSGKSHNDDSPCLHSLQPSVSSKEDGESHISCQLNLTLASSSLRI 360
           LSDTGTSFILRRDSSLSGKSHNDDSPCLHSLQPSVSSKEDGESHISCQLNLTLASSSLRI
Sbjct: 387 LSDTGTSFILRRDSSLSGKSHNDDSPCLHSLQPSVSSKEDGESHISCQLNLTLASSSLRI 446

Query: 361 NDTACSVRMPQLGQNVRKFPDSKVNSNGKESDVRLFTKNINLDEGSTPVCPQETSNNNHG 420
           NDTACSVRMPQLGQNVRKFPDSKVNSNGKESDVRLFTKNINLDEGSTPVCPQETSNNNHG
Sbjct: 447 NDTACSVRMPQLGQNVRKFPDSKVNSNGKESDVRLFTKNINLDEGSTPVCPQETSNNNHG 506

Query: 421 PPAASIRANDVFWERLLTERPGCPESEEASSNYRANPFKEPDDGTVH 468
           PPAASIRANDVFWERLLTERPGCPESEEASSNYRANPFKEPDDGTVH
Sbjct: 507 PPAASIRANDVFWERLLTERPGCPESEEASSNYRANPFKEPDDGTVH 553

BLAST of Cucsa.256700 vs. NCBI nr
Match: gi|659075760|ref|XP_008438315.1| (PREDICTED: heat stress transcription factor A-5 [Cucumis melo])

HSP 1 Score: 917.1 bits (2369), Expect = 1.2e-263
Identity = 455/467 (97.43%), Postives = 461/467 (98.72%), Query Frame = 1

Query: 1   MEAAAAGGGTGGAGPAPFLIKTYDMVDDSSTDEIVSWTSSKKSFVVWNPPEFARLLLPTF 60
           MEAAA GGGTGGAGPAPFL+KTYDMVDDSSTDEIVSWTSSKKSFVVWNPPEFARLLLPTF
Sbjct: 1   MEAAAGGGGTGGAGPAPFLLKTYDMVDDSSTDEIVSWTSSKKSFVVWNPPEFARLLLPTF 60

Query: 61  FKHSNFSSFIRQLNTYGFRKIDSEKWEFANEDFIKDQKHLLKNIHRRKPIHSHSNPQGSH 120
           FKHSNFSSFIRQLNTYGFRKIDSEKWEFANEDFIKDQKHLLKNIHRRKPIHSHSNPQGSH
Sbjct: 61  FKHSNFSSFIRQLNTYGFRKIDSEKWEFANEDFIKDQKHLLKNIHRRKPIHSHSNPQGSH 120

Query: 121 IDPERAAFEDEIERLAREKTTLETNISRFKQQKSTAKLQLQDLTVKVESMEKRQKNLLAF 180
           IDPERAAFEDEIERL+REK TLE NISRFKQQKSTAKLQLQDLTVKVESMEKRQKNLLAF
Sbjct: 121 IDPERAAFEDEIERLSREKNTLEVNISRFKQQKSTAKLQLQDLTVKVESMEKRQKNLLAF 180

Query: 181 LEKAVQNPSFVEHLARRVESMDFTAFKKKRRLPSADLSQPVVENSFLDNHSSSRSESGNI 240
           LEKAVQNPSFVEHLARRVESMDFTAFKKKRRLPSADLSQPV+ENSFLDNHSSSRSESGNI
Sbjct: 181 LEKAVQNPSFVEHLARRVESMDFTAFKKKRRLPSADLSQPVIENSFLDNHSSSRSESGNI 240

Query: 241 FHQDFSQKLRLETSCASDINLISRSTQSSNEEGGSSQRQLSKFDTRAVQENIHFAVETLD 300
           FHQDFSQKLRLETSCASDINLISRSTQSSNEEGGSSQRQLSKFDTRAVQEN+HFA ETLD
Sbjct: 241 FHQDFSQKLRLETSCASDINLISRSTQSSNEEGGSSQRQLSKFDTRAVQENLHFAAETLD 300

Query: 301 LSDTGTSFILRRDSSLSGKSHNDDSPCLHSLQPSVSSKEDGESHISCQLNLTLASSSLRI 360
           LSDTGTSFILRRDSSLSGKSHNDD+P LHSLQPSVSSKEDGESHISCQLNLTLASSSLRI
Sbjct: 301 LSDTGTSFILRRDSSLSGKSHNDDNPRLHSLQPSVSSKEDGESHISCQLNLTLASSSLRI 360

Query: 361 NDTACSVRMPQLGQNVRKFPDSKVNSNGKESDVRLFTKNINLDEGSTPVCPQETSNNNHG 420
           NDTACSVRMPQLGQNVRKFPDSK NSNGKESDVRLFTKNINLDEGSTPVCPQETSNNNHG
Sbjct: 361 NDTACSVRMPQLGQNVRKFPDSKANSNGKESDVRLFTKNINLDEGSTPVCPQETSNNNHG 420

Query: 421 PPAASIRANDVFWERLLTERPGCPESEEASSNYRANPFKEPDDGTVH 468
           PPAASIRANDVFWERLLTERPGCPESEEASSNYRANP+KEPDDGTVH
Sbjct: 421 PPAASIRANDVFWERLLTERPGCPESEEASSNYRANPYKEPDDGTVH 467

BLAST of Cucsa.256700 vs. NCBI nr
Match: gi|645232304|ref|XP_008222805.1| (PREDICTED: heat stress transcription factor A-5 [Prunus mume])

HSP 1 Score: 632.9 bits (1631), Expect = 4.5e-178
Identity = 319/468 (68.16%), Postives = 377/468 (80.56%), Query Frame = 1

Query: 1   MEAAAAGGGTGGAGPAPFLIKTYDMVDDSSTDEIVSWTSSKKSFVVWNPPEFARLLLPTF 60
           ME A+  GG  G GPAPFL+KTYDMVDDS+TDEIVSW+++KKSF+VWNPPEFARLLLPT+
Sbjct: 1   MEGASTAGG--GGGPAPFLLKTYDMVDDSATDEIVSWSTNKKSFIVWNPPEFARLLLPTY 60

Query: 61  FKHSNFSSFIRQLNTYGFRKIDSEKWEFANEDFIKDQKHLLKNIHRRKPIHSHSNPQGSH 120
           FKH+NFSSFIRQLNTYGFRKID E+WEFANEDFI+DQKHLLKNIHRRKPIHSHSNPQGS 
Sbjct: 61  FKHNNFSSFIRQLNTYGFRKIDPERWEFANEDFIQDQKHLLKNIHRRKPIHSHSNPQGSM 120

Query: 121 IDPERAAFEDEIERLAREKTTLETNISRFKQQKSTAKLQLQDLTVKVESMEKRQKNLLAF 180
           +DPERAA +DEIE+L+ +K TLE NISRFKQQ+S AKLQL+DLT +V SME+RQK+LL F
Sbjct: 121 VDPERAALDDEIEKLSHDKATLEANISRFKQQRSDAKLQLEDLTQRVNSMEQRQKDLLKF 180

Query: 181 LEKAVQNPSFVEHLARRVESMDFTAFKKKRRLPSADLSQPVVENSFLDNHSSSRSESGNI 240
           L+K VQNP+FVEHL R++E+MDF+A  KKRRLP  D  QPVVENSF+DN SSSRSE GNI
Sbjct: 181 LDKNVQNPTFVEHLTRKIEAMDFSACNKKRRLPDVDHLQPVVENSFVDNQSSSRSEFGNI 240

Query: 241 FHQDFSQKLRLETSCA-SDINLISRSTQSSNEEGGSSQRQLSKFDTRAVQ---ENIHFAV 300
           FHQDFS KLRLE S A SDINL+SRSTQSSNE+G S  R++S+ + + VQ   E + FA 
Sbjct: 241 FHQDFSSKLRLELSPAVSDINLVSRSTQSSNEDGYSPTRKISE-ELKGVQKRTEGLLFAP 300

Query: 301 ETLDLSDTGTSFILRRDSSLSGKSHNDDSPCLHSLQPSVSSKEDGESHISCQLNLTLASS 360
           ETL+LSDTGTSF  + DS LS K+    +P LHSLQP +SS E+G+  ISCQL LTLASS
Sbjct: 301 ETLELSDTGTSFAFKMDSLLSRKALTVGNPRLHSLQPGLSSNEEGDGQISCQLKLTLASS 360

Query: 361 SLRINDTACSVRMPQLGQNVRKFPDSKVNSNGKESDVRLFTKNINLDEGSTPVCPQETSN 420
            L++N +  S  +PQ+GQ++ K   S +N+ GKESD+R FT     DE     C QE +N
Sbjct: 361 PLQVNSSPHSATIPQVGQDISKSLASGLNAIGKESDIRAFTNKNPADEDMHKTCSQEATN 420

Query: 421 NNHGPPAASIRANDVFWERLLTERPGCPESEEASSNYRANPFKEPDDG 465
           NN GPP A +R NDVFWE+ LTERPGC E+EEASSNYR NP+ E DDG
Sbjct: 421 NNQGPPPAPVRVNDVFWEQFLTERPGCSENEEASSNYRGNPYDEQDDG 465

BLAST of Cucsa.256700 vs. NCBI nr
Match: gi|658006835|ref|XP_008338589.1| (PREDICTED: heat stress transcription factor A-5-like [Malus domestica])

HSP 1 Score: 621.3 bits (1601), Expect = 1.4e-174
Identity = 307/468 (65.60%), Postives = 371/468 (79.27%), Query Frame = 1

Query: 1   MEAAAAGGGTGGAGPAPFLIKTYDMVDDSSTDEIVSWTSSKKSFVVWNPPEFARLLLPTF 60
           ME  +  GG  G GPAPFL+KTYDMVDDS+TDEIVSW+S+KKSF+VWNPPEFAR+LLPT+
Sbjct: 1   MEGPSTAGG--GGGPAPFLLKTYDMVDDSATDEIVSWSSNKKSFIVWNPPEFARVLLPTY 60

Query: 61  FKHSNFSSFIRQLNTYGFRKIDSEKWEFANEDFIKDQKHLLKNIHRRKPIHSHSNPQGSH 120
           FKH+NFSSFIRQLNTYGFRKID E+WEFANEDFI+DQKHLLKNIHRRKPIHSHSNPQGS 
Sbjct: 61  FKHNNFSSFIRQLNTYGFRKIDPERWEFANEDFIQDQKHLLKNIHRRKPIHSHSNPQGSM 120

Query: 121 IDPERAAFEDEIERLAREKTTLETNISRFKQQKSTAKLQLQDLTVKVESMEKRQKNLLAF 180
           +D ERAA +DEIE+L+ +K  LE NISRFKQQ+S AKLQL+DLT +V  ME+RQKNL+ F
Sbjct: 121 VDSERAALDDEIEKLSHDKAALEANISRFKQQRSAAKLQLEDLTQRVNGMEQRQKNLVTF 180

Query: 181 LEKAVQNPSFVEHLARRVESMDFTAFKKKRRLPSADLSQPVVENSFLDNHSSSRSESGNI 240
           L++AV NP+FVEHL R++ESMDF+A+ KKRRLP  D  QPVVEN F+DN SSSRSE GNI
Sbjct: 181 LDRAVHNPNFVEHLTRKIESMDFSAYHKKRRLPDIDHLQPVVENGFVDNRSSSRSEFGNI 240

Query: 241 FHQDFSQKLRLETSCA-SDINLISRSTQSSNEEGGSSQRQLSKFDTRAVQ---ENIHFAV 300
           FHQDFS KLRLE S A SD+NL+SRSTQSSNE+GGSS R++S+ + +  Q   E + FA 
Sbjct: 241 FHQDFSSKLRLELSPAVSDMNLVSRSTQSSNEDGGSSTRKISE-ELKGAQMRPEGVLFAP 300

Query: 301 ETLDLSDTGTSFILRRDSSLSGKSHNDDSPCLHSLQPSVSSKEDGESHISCQLNLTLASS 360
           ETL+LSDTGTSF  + DS LS K+    SP  HSLQP + S E+G+ HISC LNLTLAS+
Sbjct: 301 ETLELSDTGTSFAFKMDSLLSRKAPTVGSPRRHSLQPGLPSNEEGDGHISCHLNLTLAST 360

Query: 361 SLRINDTACSVRMPQLGQNVRKFPDSKVNSNGKESDVRLFTKNINLDEGSTPVCPQETSN 420
            L++N++ C   +PQ+GQ++ +   S++N NGK+SD+R FT     D        QE ++
Sbjct: 361 PLQVNNSPCLATVPQVGQDINESAASELNENGKDSDLRAFTNKNIADNDKLKTSSQEATH 420

Query: 421 NNHGPPAASIRANDVFWERLLTERPGCPESEEASSNYRANPFKEPDDG 465
           NN  PP   +R NDVFWE+ LTERPGC E+EEASS+YR NPF E DDG
Sbjct: 421 NNQAPPLVPVRVNDVFWEQFLTERPGCSENEEASSDYRGNPFDEQDDG 465

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
HSFA5_ARATH1.3e-12151.50Heat stress transcription factor A-5 OS=Arabidopsis thaliana GN=HSFA5 PE=2 SV=1[more]
HSFA5_ORYSJ1.1e-9946.60Heat stress transcription factor A-5 OS=Oryza sativa subsp. japonica GN=HSFA5 PE... [more]
HFA4A_ARATH4.0e-5743.82Heat stress transcription factor A-4a OS=Arabidopsis thaliana GN=HSFA4A PE=2 SV=... [more]
HFA4B_ORYSJ3.4e-5633.33Heat stress transcription factor A-4b OS=Oryza sativa subsp. japonica GN=HSFA4B ... [more]
HFA4D_ORYSJ9.8e-5652.80Heat stress transcription factor A-4d OS=Oryza sativa subsp. japonica GN=HSFA4D ... [more]
Match NameE-valueIdentityDescription
A0A0A0L9B5_CUCSA2.3e-269100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_3G129630 PE=3 SV=1[more]
A0A061ES53_THECC3.3e-16764.52Winged-helix DNA-binding transcription factor family protein OS=Theobroma cacao ... [more]
B9S9Y4_RICCO3.1e-16564.03DNA binding protein, putative OS=Ricinus communis GN=RCOM_0523380 PE=3 SV=1[more]
B9GFJ0_POPTR1.7e-16362.85Heat Stress Transcription Factor family protein OS=Populus trichocarpa GN=POPTR_... [more]
A0A067L5F4_JATCU1.2e-16163.52Uncharacterized protein OS=Jatropha curcas GN=JCGZ_01081 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G13980.17.5e-12351.50 winged-helix DNA-binding transcription factor family protein[more]
AT4G18880.12.2e-5843.82 heat shock transcription factor A4A[more]
AT1G32330.14.4e-5439.52 heat shock transcription factor A1D[more]
AT5G16820.11.3e-5348.80 heat shock factor 3[more]
AT4G17750.12.2e-5344.58 heat shock factor 1[more]
Match NameE-valueIdentityDescription
gi|449433171|ref|XP_004134371.1|3.3e-269100.00PREDICTED: heat stress transcription factor A-5 [Cucumis sativus][more]
gi|700201573|gb|KGN56706.1|3.3e-269100.00hypothetical protein Csa_3G129630 [Cucumis sativus][more]
gi|659075760|ref|XP_008438315.1|1.2e-26397.43PREDICTED: heat stress transcription factor A-5 [Cucumis melo][more]
gi|645232304|ref|XP_008222805.1|4.5e-17868.16PREDICTED: heat stress transcription factor A-5 [Prunus mume][more]
gi|658006835|ref|XP_008338589.1|1.4e-17465.60PREDICTED: heat stress transcription factor A-5-like [Malus domestica][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000232HSF_DNA-bd
IPR011991Winged helix-turn-helix DNA-binding domain
IPR027725HSF_fam
Vocabulary: Molecular Function
TermDefinition
GO:0003700transcription factor activity, sequence-specific DNA binding
GO:0043565sequence-specific DNA binding
Vocabulary: Biological Process
TermDefinition
GO:0006355regulation of transcription, DNA-templated
Vocabulary: Cellular Component
TermDefinition
GO:0005634nucleus
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
biological_process GO:0009408 response to heat
cellular_component GO:0005634 nucleus
cellular_component GO:0005667 transcription factor complex
molecular_function GO:0043565 sequence-specific DNA binding
molecular_function GO:0003700 transcription factor activity, sequence-specific DNA binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.256700.1Cucsa.256700.1mRNA


Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000232Heat shock factor (HSF)-type, DNA-bindingPRINTSPR00056HSFDOMAINcoord: 18..41
score: 8.1E-18coord: 69..81
score: 8.1E-18coord: 56..68
score: 8.1
IPR000232Heat shock factor (HSF)-type, DNA-bindingPFAMPF00447HSF_DNA-bindcoord: 18..107
score: 1.6
IPR000232Heat shock factor (HSF)-type, DNA-bindingSMARTSM00415hsfneu3coord: 14..107
score: 6.6
IPR000232Heat shock factor (HSF)-type, DNA-bindingPROSITEPS00434HSF_DOMAINcoord: 57..81
scor
IPR011991Winged helix-turn-helix DNA-binding domainGENE3DG3DSA:1.10.10.10coord: 14..107
score: 1.3
IPR011991Winged helix-turn-helix DNA-binding domainunknownSSF46785"Winged helix" DNA-binding domaincoord: 14..107
score: 8.84
IPR027725Heat shock transcription factor familyPANTHERPTHR10015HEAT SHOCK TRANSCRIPTION FACTORcoord: 2..463
score: 1.1E
NoneNo IPR availableunknownCoilCoilcoord: 125..173
scor
NoneNo IPR availablePANTHERPTHR10015:SF157HEAT STRESS TRANSCRIPTION FACTOR A-5coord: 2..463
score: 1.1E

The following gene(s) are paralogous to this gene:

None