BLAST of Cucsa.256480 vs. Swiss-Prot
Match:
UBP2_ARATH (Ubiquitin carboxyl-terminal hydrolase 2 OS=Arabidopsis thaliana GN=UBP2 PE=1 SV=2)
HSP 1 Score: 538.5 bits (1386), Expect = 1.5e-151
Identity = 341/859 (39.70%), Postives = 461/859 (53.67%), Query Frame = 1
Query: 1 MGKKVKKKTNRAPPKEKRVSSSSPRKVPQPSNTTVETADEEISIVKEKSQCGHLDKCFNL 60
MGKK KKK RAP KE + S + +P + E A+ ++ VKE C H DK NL
Sbjct: 1 MGKKAKKKA-RAPTKEIQTMEISKKVSEEPPSQAGEIAEGDVKAVKETQACVHFDKALNL 60
Query: 61 NELSSKLGSAEPISCEDCQDSSADRRGGRGKARHGKKKGGTSVDVKTDAKAIWICLQCGH 120
++ K+ S+ I C +C + +RG + K GKK +S D K++ KAIW+CL+CG
Sbjct: 61 EKVLDKIKSSRQIKCAECNEGVYGKRGTKAKGSKGKKDFSSS-DPKSNNKAIWLCLECGC 120
Query: 121 YACGGIGLPTNSQSHAVRHVRQTRHPVVIQFENPQLRWCFSCNTLLPVEKTEENGEQKDS 180
Y CGG+GLP QSH +RH R TRH +VIQ+ENPQLRWCF C LLPVEK E+NGE+KD
Sbjct: 121 YVCGGVGLPNGPQSHVLRHSRVTRHRLVIQWENPQLRWCFPCQLLLPVEK-EDNGEKKDV 180
Query: 181 LSNVVKLIRDRSMES-THVDVENTRYTSSEVTAATKPESSVLSDLDRRNQYIVKGLINLG 240
LS VVKLI+ RS+ + D+E+ S +T+ K E +V SD++ R+ Y+V+GL+NLG
Sbjct: 181 LSEVVKLIKGRSLNNLASSDIEDQCSGSGSITSDIKLEGAVTSDIEARDGYVVRGLVNLG 240
Query: 241 NTCFFNSILQNLLAIDMLRDHFVKLEECV--GPLTIALKKIFIEARTESRMKSSINPRSV 300
NTCFFNSI+QNLL++D LRDHF+K GPL +L+K+F E + E+ +KS INPR+
Sbjct: 241 NTCFFNSIMQNLLSLDRLRDHFLKENGSGVGGPLASSLRKLFTETKPEAGLKSVINPRAF 300
Query: 301 FGCISSKAPQFKGYEQHDSHELLRVLLDALSSEELTSRKMTNSKEERISGNPTPTFVDEM 360
FG SKAPQF+GY+QHDSHELLR LLD+LS+EE RK + + + T ++ +
Sbjct: 301 FGSFCSKAPQFRGYDQHDSHELLRCLLDSLSTEESALRKKRGVSD---NDEKSTTLIESV 360
Query: 361 FGGQISSAVCCKECGHTSTVYEPFLDLSLPVPMKKPLAKKVQPVSRAKKTKVPPKRNGKT 420
FGG+ SS V C ECGH+S VYEPFLDLSLPVP KK KK QPVSRAKK K+PPKR K
Sbjct: 361 FGGETSSIVSCMECGHSSKVYEPFLDLSLPVPFKKSPPKKPQPVSRAKKAKLPPKRVPKN 420
Query: 421 IPKTNKVSDIVPIQIAS--------------------------------VPSSSNESSLP 480
+ K +KVS ++P + S PS +
Sbjct: 421 VSKVSKVSKVLPGMVLSELNSSGKSMAVTADSDTSCSSLAPLDNGPVLETPSVLTLDNNQ 480
Query: 481 SEASASSTTT---------IMEKTSTSQNVSDAKESGKE-------------------IS 540
+ SAS + T I +TS + D +E G + S
Sbjct: 481 ASESASQSDTGFDGSWLDFIGPETSGDETNLDMQEDGIDNVITAEVNQIVPSPNIVANSS 540
Query: 541 VENGGECASDNLT-----WMDFLEPELNADNCDISTTQDSENNIEVFISDNSQQSVSGLS 600
V +G + N + + + E N D D+ Q E IS Q+
Sbjct: 541 VSSGDQTLEGNTERLMQDYEEIAKAEANLDEKDVQAMQSDECPATSGISAEFSQA----- 600
Query: 601 MPVSSLHSEPNQRPDFSSVNSWNDEA-PLQVQASEVLLLPYKEESSTAEVAKEDDQASSS 660
S + +P SSVN W++E PL V S++L +PYKE S + + + +ASSS
Sbjct: 601 ---SCIGCDPGIGESSSSVNPWDEEELPLVVADSQILYMPYKEISCNDKSVEGECEASSS 660
Query: 661 ILGCAQE--DFDGFGLGDMFDEPEIPIGPIGRPSTSNE---VAESSFNSESDPEEVDNTD 720
+ E + D G +FDEPE GP+ P + E V +F+SESDPEE+D++D
Sbjct: 661 FVTGDHEPQNSDFVDFGGLFDEPETTEGPVFGPPSKAEASGVGFMAFSSESDPEEIDDSD 720
Query: 721 APVSVESCLTFFTKPELLSNENGYNCEKCSKRLQQQRLEMKKQSKVACKAVANGCQTAVG 780
PVSVE CL FTK E+LS++N +NCE CSK L+ QRL K++S
Sbjct: 721 LPVSVERCLGHFTKHEILSDDNAWNCENCSKNLKLQRLREKRKS---------------- 780
HSP 2 Score: 190.7 bits (483), Expect = 7.8e-47
Identity = 110/239 (46.03%), Postives = 152/239 (63.60%), Query Frame = 1
Query: 752 QDCSKPVNCQKSKTDPPVLDEDEAKVDKDMNPGLSRSSGCNNTRNQENSDD---KSSCSL 811
++CSK + Q+ + + +EDE SRSS +N +EN D+ ++
Sbjct: 733 ENCSKNLKLQRLR-EKRKSNEDE-----------SRSSNTSNGWVKENEDEGFGETEILA 792
Query: 812 PNDEPAKTNI--EHLSS-------HLAVGNQSEKSEDGEMDSDSTIVNVKRDATKRFLIH 871
+P T+ +H S H A ++S+ ++D + DS+ ++ VKRDATK+ LI+
Sbjct: 793 VKQDPNDTSCVKDHSSDGRKAARIHSADESESKGTQDEDEDSEK-VITVKRDATKKVLIN 852
Query: 872 KAPPILTIHMKRFSPDARGRYSKLNGHVRFKETIDLKPYLDTRCADRDKCSYRLVGVVEH 931
KAPP+LTIH+KRFS D RGR SKLNGHV FKE IDL+ Y+D+RC+ D YRL G+VEH
Sbjct: 853 KAPPVLTIHLKRFSQDLRGRLSKLNGHVAFKEVIDLRQYMDSRCSGEDPPVYRLAGLVEH 912
Query: 932 SGSMRGGHYVAYVRGGNRKRSSGEAEEDASVWYYASDAVVDEVTLDRVLGCEAYILFYE 979
SG+MRGGHYVAYVRGG R + E + ++ WY SDA V +V+L++VL EAYILFYE
Sbjct: 913 SGTMRGGHYVAYVRGGQRVK---ETDSSSTAWYNVSDAYVRQVSLEKVLHSEAYILFYE 955
BLAST of Cucsa.256480 vs. Swiss-Prot
Match:
UBP1_ARATH (Ubiquitin carboxyl-terminal hydrolase 1 OS=Arabidopsis thaliana GN=UBP1 PE=1 SV=2)
HSP 1 Score: 378.6 bits (971), Expect = 2.0e-103
Identity = 238/617 (38.57%), Postives = 343/617 (55.59%), Query Frame = 1
Query: 31 SNTTVETADEEISIVKEKSQCGHLDKCFNLNELSSKLGSAEPISCEDCQDSSADRRGGRG 90
S +VE +E + EK C H DK +L++L + S + I C +C + +RG R
Sbjct: 12 SEQSVEVTEEFAVTMTEKRSCVHFDKYVDLDKLLKIIKSYQQIKCGECNERVHVKRGSRW 71
Query: 91 KARHGKKKGGTSVDVKTDAKAIWICLQCGHYACGGIGLPTNSQSHAVRHVRQTRHPVVIQ 150
A + ++ +S D AIW+CL+CG+Y CG +GLPT +QSH + H R TRH +VIQ
Sbjct: 72 GASN-RRDWYSSSDQNCARNAIWLCLECGYYVCGDVGLPTEAQSHVMGHNRLTRHRLVIQ 131
Query: 151 FENPQLRWCFSCNTLLPVEKTEENGEQKDSLSNVVKLIRDRSMESTHVDVENTRYTSSEV 210
+NPQLRWCFSC +LLP + EENGE+KD L VVKLIR+RS + E T ++ S
Sbjct: 132 CKNPQLRWCFSCQSLLPFD-NEENGEKKDLLLEVVKLIRERSPNTYSASFE-TEFSCS-- 191
Query: 211 TAATKPESSVLSDLDRRNQYIVKGLINLGNTCFFNSILQNLLAIDMLRDHFVKLEECV-G 270
S+ ++ R+ Y V+GL+NLGNTCFFNS++QNLL++D LR+HF+K + V G
Sbjct: 192 -------GSIYGGIEARDGYAVRGLVNLGNTCFFNSVMQNLLSLDQLREHFLKEDLSVSG 251
Query: 271 PLTIALKKIFIEARTE-SRMKSSINPRSVFGCISSKAPQFKGYEQHDSHELLRVLLDALS 330
PL +LK++F E+ +E S ++ INPR +F + S+APQF+GY+QHDSHELLR LLD LS
Sbjct: 252 PLVSSLKELFAESNSEASVFRNEINPRDLFFSVCSQAPQFRGYQQHDSHELLRCLLDGLS 311
Query: 331 SEELTSRKMTNSKEERISGN-PTPTFVDEMFGGQISSAVCCKECGHTSTVYEPFLDLSLP 390
EE + RK + S PT +D +FGG+ISS V C ECGH S VYEPF+DLSLP
Sbjct: 312 IEESSLRKKLGVSDSNDSSTYQKPTLIDSVFGGEISSTVSCLECGHFSKVYEPFMDLSLP 371
Query: 391 VPMKKPLAKKVQPVSRAKKTKVPPKRNGKTIPKTNKVSDIVPIQIASVPSSSNESSLPSE 450
VP K KK Q +S+AK+ +NG + K ++V +++ P+S + S++P
Sbjct: 372 VPSMKLPPKKQQILSQAKEV----LKNG-AVSKDSEV-------VSAKPASDHNSTVP-- 431
Query: 451 ASASSTTTIMEKTSTSQNVSDAKESGKEISVENGGECASDNLTWMDFLEPELNADNCDIS 510
S I + TS N +D E N + E ++ ++
Sbjct: 432 -LFPSDHKIQSRPETSDNETDLVLLSDVSDTAPSTEAKGVNQILVGSTETLMHDNDRTGK 491
Query: 511 TTQDSENNIEVFISDNSQQSVSGLSMPV--SSLHSEPNQRPDFSSVNSWND-EAPLQVQA 570
T D E+ N + S SG+S + + + P+ SS N D E L V
Sbjct: 492 TVPDKEDVRAT--QSNEETSASGISAVIDEAQVCGCPDLEQSSSSANQGADEELALMVAD 551
Query: 571 SEVLLLPYKEESSTAEVAKEDDQASSSILGCAQEDFDGFGLGDMFDEPEIPIGPIGRPST 630
S+VL +PYK+ + + +SS + G + D F DEPEI GP+ RP +
Sbjct: 552 SQVLYMPYKDHLFYDDYMVA-EASSSFVSGDHEPKKDYFDFSSFLDEPEIREGPVFRPLS 598
Query: 631 SNEVAESSFNSESDPEE 642
+EV E+ F ++ ++
Sbjct: 612 KSEVYEAGFKADCSDDK 598
HSP 2 Score: 364.0 bits (933), Expect = 5.1e-99
Identity = 265/815 (32.52%), Postives = 409/815 (50.18%), Query Frame = 1
Query: 31 SNTTVETADEEISIVKEKSQCGHLDKCFNLNELSSKLGSAEPISCEDCQDSSADRRGGRG 90
S +VE +E + EK C H DK +L++L + S + I C +C + +RG R
Sbjct: 12 SEQSVEVTEEFAVTMTEKRSCVHFDKYVDLDKLLKIIKSYQQIKCGECNERVHVKRGSRW 71
Query: 91 KARHGKKKGGTSVDVKTDAKAIWICLQCGHYACGGIGLPTNSQSHAVRHVRQTRHPVVIQ 150
A + ++ +S D AIW+CL+CG+Y CG +GLPT +QSH + H R TRH +VIQ
Sbjct: 72 GASN-RRDWYSSSDQNCARNAIWLCLECGYYVCGDVGLPTEAQSHVMGHNRLTRHRLVIQ 131
Query: 151 FENPQLRWCFSCNTLLPVEKTEENGEQKDSLSNVVKLIRDRSMESTHVDVENTRYTSSEV 210
+NPQLRWCFSC +LLP + EENGE+KD L VVKLIR+RS + E T ++ S
Sbjct: 132 CKNPQLRWCFSCQSLLPFDN-EENGEKKDLLLEVVKLIRERSPNTYSASFE-TEFSCS-- 191
Query: 211 TAATKPESSVLSDLDRRNQYIVKGLINLGNTCFFNSILQNLLAIDMLRDHFVKLEECV-G 270
S+ ++ R+ Y V+GL+NLGNTCFFNS++QNLL++D LR+HF+K + V G
Sbjct: 192 -------GSIYGGIEARDGYAVRGLVNLGNTCFFNSVMQNLLSLDQLREHFLKEDLSVSG 251
Query: 271 PLTIALKKIFIEARTESRM-KSSINPRSVFGCISSKAPQFKGYEQHDSHELLRVLLDALS 330
PL +LK++F E+ +E+ + ++ INPR +F + S+APQF+GY+QHDSHELLR LLD LS
Sbjct: 252 PLVSSLKELFAESNSEASVFRNEINPRDLFFSVCSQAPQFRGYQQHDSHELLRCLLDGLS 311
Query: 331 SEELTSRKMTNSKEERISGN-PTPTFVDEMFGGQISSAVCCKECGHTSTVYEPFLDLSLP 390
EE + RK + S PT +D +FGG+ISS V C ECGH S VYEPF+DLSLP
Sbjct: 312 IEESSLRKKLGVSDSNDSSTYQKPTLIDSVFGGEISSTVSCLECGHFSKVYEPFMDLSLP 371
Query: 391 VPMKKPLAKKVQPVSRAKK--------------TKVPPKRNGKTIP-------------K 450
VP K KK Q +S+AK+ + P + T+P
Sbjct: 372 VPSMKLPPKKQQILSQAKEVLKNGAVSKDSEVVSAKPASDHNSTVPLFPSDHKIQSRPET 431
Query: 451 TNKVSDIVPIQIASVPSSSNESSLPSEASASSTTTIM-EKTSTSQNVSD-----AKESGK 510
++ +D+V + S + S E+ ++ ST T+M + T + V D A +S +
Sbjct: 432 SDNETDLVLLSDVSDTAPSTEAKGVNQILVGSTETLMHDNDRTGKTVPDKEDVRATQSNE 491
Query: 511 EISVENGGECASDNLTWMDFLEPELNADNCDISTTQDSENNIEVFISDNS---------- 570
E S +G D + E ++ S Q ++ + + ++D+
Sbjct: 492 ETSA-SGISAVIDEAQVCGCPDLEQSSS----SANQGADEELALMVADSQVLYMPYKDHL 551
Query: 571 ---QQSVSGLSMPVSSLHSEPNQRPDFSSVNSWNDEAPLQ-------VQASEVLLLPYKE 630
V+ S S EP + D+ +S+ DE ++ + SEV +K
Sbjct: 552 FYDDYMVAEASSSFVSGDHEPKK--DYFDFSSFLDEPEIREGPVFRPLSKSEVYEAGFKA 611
Query: 631 ESSTAEV--AKEDDQASSSILGCAQEDFDGFGLGDMFDEPEIPIGPIGRPSTSNEVAESS 690
+ S + A + + +SS I +++ D FDEPEI P RP + +EV+E+
Sbjct: 612 DCSDDKTVSAGKGEASSSFISSDHEQNIDYVDFSSFFDEPEISERPFFRPLSKSEVSEAG 671
Query: 691 FNSESDPEEVDNTDAPVSVESCLTFFTKP-----ELLSNENGYNCEKCSKRLQQQRLEMK 750
F ++ D T + E+ +F + + + + ++ + S+R + L
Sbjct: 672 FKADCSD---DKTVSAGKGEASSSFVSSDHEQNIDYVDFSSFFDEPEISERPFFRPLSKS 731
Query: 751 KQSKVACKAVANGCQTAVGGDISSCNEDSSVEVKNKRNMNLT--NGSISYSSGESSNLKK 781
+ S+ AV+ +T G + + S + N+RN++ I G S
Sbjct: 732 EVSEAGFMAVSGNDKTVRAGKGETFS--SFMSGDNERNIDYVEFTNRIFDDRGTSERPVF 791
HSP 3 Score: 186.4 bits (472), Expect = 1.5e-45
Identity = 160/546 (29.30%), Postives = 255/546 (46.70%), Query Frame = 1
Query: 461 STSQNVSDAKESGKEISVENGGECASDNLTWMDFL-EPELNADNC--DISTTQDSENNIE 520
S + VS K + + E D + + F EPE++ +S ++ SE +
Sbjct: 595 SDDKTVSAGKGEASSSFISSDHEQNIDYVDFSSFFDEPEISERPFFRPLSKSEVSEAGFK 654
Query: 521 VFISDNSQQSVSGLSMPVSSLHSEPNQRPDFSSVNSWNDEAPLQVQASEVLLLPYKEESS 580
SD+ S S + S+ Q D+ +S+ DE E+ P+ S
Sbjct: 655 ADCSDDKTVSAGKGEASSSFVSSDHEQNIDYVDFSSFFDEP-------EISERPFFRPLS 714
Query: 581 TAEVAKEDDQASS----SILGCAQEDFDGFGLGD-------------MFDEPEIPIGPIG 640
+EV++ A S ++ E F F GD +FD+ P+
Sbjct: 715 KSEVSEAGFMAVSGNDKTVRAGKGETFSSFMSGDNERNIDYVEFTNRIFDDRGTSERPVF 774
Query: 641 RPSTSNEVAESSF---NSESDPEEVDNTDAPVSVESCLTFFTKPELLSNENGYNCEKCSK 700
P + +V+E+ F +S+SDP +D +++ + ++C
Sbjct: 775 GPPSKAKVSEAGFVAVSSDSDPAVLD---------------------ESDSPVSVDRCLA 834
Query: 701 RLQQQRLEMKKQSKVACKAVANGCQTAVGGDISSCNEDSSVEVKNKRNMNLTNGSISYSS 760
+ + + + + + C+ + + + K + L+N ++ +
Sbjct: 835 QFTKHEI-LSEDNAWHCENCSKNLKL------------QRLREKRRTKEGLSNRWVN-EN 894
Query: 761 GESSNLKKNVDCS-SQDCSKPVNCQKSK---TDPPVLDEDEAKVDKDMNPGLSRSSGCNN 820
G SS + D S +Q C N K+ T P E+E+ +D +
Sbjct: 895 GASSAFDECRDSSLNQSCIDLENGYKAAPPITKLPNCKEEESAID-------------DG 954
Query: 821 TRNQENSDDKSSCSLPNDEPAKTNIEHLSSHLAVGNQSEKSEDGEMDSDSTIVNVKRDAT 880
+EN+ S+ E E +SS A N+ E ED +DS+ IV KRDA
Sbjct: 955 FVGEENTKQAPITSVT--ETPLLGGETISSQPASDNECENWEDLAVDSEEVIV--KRDAR 1014
Query: 881 KRFLIHKAPPILTIHMKRFSPDARGRYSKLNGHVRFKETIDLKPYLDTRCADRDKCSYRL 940
K+ LI+KAPP+LTIH+KRFS DARGR SKL+GHV F+E IDL Y+DTRC++ D+ YRL
Sbjct: 1015 KKVLINKAPPVLTIHLKRFSQDARGRVSKLSGHVDFQEFIDLSKYMDTRCSEEDEPVYRL 1074
Query: 941 VGVVEHSGSMRGGHYVAYVRGGNRKRSSGEAEE-DASVWYYASDAVVDEVTLDRVLGCEA 979
G+VEH G+M GHYV+Y+RGG+++R + +E ++S+WY+ASD+ V +L+ VL EA
Sbjct: 1075 AGLVEHLGAMSRGHYVSYIRGGHKERRDSDTKEPNSSIWYHASDSQVRPASLEEVLRSEA 1081
BLAST of Cucsa.256480 vs. Swiss-Prot
Match:
UBP16_RAT (Ubiquitin carboxyl-terminal hydrolase 16 OS=Rattus norvegicus GN=Usp16 PE=1 SV=2)
HSP 1 Score: 181.4 bits (459), Expect = 4.7e-44
Identity = 168/706 (23.80%), Postives = 291/706 (41.22%), Query Frame = 1
Query: 33 TTVETADEEISIVKEKSQCGHLDKCFNLNELSSKLGSAEPISCEDCQDSSADRRGGRGKA 92
T ++ E+ S + C HL K L L + E C+DC+ +
Sbjct: 6 TKGKSVPEKASSESTEPMCRHLRKGLEQGNLKKALVNVEWNICQDCKTDN---------- 65
Query: 93 RHGKKKGGTSVDVKTDAKAIWICLQCGHYACGGIGLPTNSQSHAVRHVRQTR---HPVVI 152
K + + + ++W+CL+CGH CG + + HA++H R H +V+
Sbjct: 66 -----KVKDKSEEEAEDPSVWLCLKCGHQGCGR----DSQEQHALKHYTTPRSEPHYLVL 125
Query: 153 QFENPQLRWCFSCNTLLPVEKTEENGEQKDSLSNVVKLIRDRSMEST--HVDVENTRYTS 212
+N + WC+ C+ + + G+ D + I + E H+++EN +
Sbjct: 126 SLDNWSV-WCYKCDEEIKYCSSNRLGQVVDYVRKQAGRITSKPAEKNNGHIELENKKLEK 185
Query: 213 SEVTAATKPESSVLSD----LDRRNQYIVKGLINLGNTCFFNSILQNLLAIDMLRD---- 272
+ +S ++ LD +Q VKGL NLGNTCFFN+++QNL +LR+
Sbjct: 186 ESKNEQEREKSESMAKENIPLDSASQITVKGLSNLGNTCFFNAVMQNLSQTPVLRELLKE 245
Query: 273 -----HFVKLE--------------ECVGPLTIALKKIFIEARTESRMKSSINPRSVFGC 332
VK+E E GPLT+A+ + E + K + P+ +F
Sbjct: 246 VKMSGTIVKIEPPDLALTEPLEVNLEPPGPLTLAMSQFLNEM--QENKKRIVTPKELFSQ 305
Query: 333 ISSKAPQFKGYEQHDSHELLRVLLDALSSEE-----------------LTSRKMTNSKEE 392
+ KA +FKGY+Q DS ELLR LLD + +EE ++ N ++
Sbjct: 306 VCKKATRFKGYQQQDSQELLRYLLDGMRAEEHQRVSKGILKAFGNSTEKLDEEVKNKVKD 365
Query: 393 RISGNPTPTFVDEMFGGQISSAVCCKECGHTSTVYEPFLDLSLPVPMKKPLAKKVQPVSR 452
P+FVD +FGG+++S + C +C S V+E FLDLSLPV + K +
Sbjct: 366 YEKKKAIPSFVDRIFGGELTSTIMCDDCRTVSLVHESFLDLSLPVLDDQSGKKNI----- 425
Query: 453 AKKTKVPPKRNGKTIPKTNKVSDIVPIQIASVPSSSNESSLPSEASASSTTTIMEKTSTS 512
N K + KT + D + S +P S T+ +K +
Sbjct: 426 ----------NDKNVKKTMEEEDKDSEEEKDDSYMKTRSDVP-----SGTSKHTQKKAKK 485
Query: 513 QNVSDAKESGKEISVE----NGGECASDNLTWMDFLEPEL---NADNCDISTTQDSENNI 572
Q AK ++ ++ + E + N T + E E D + S+ +
Sbjct: 486 QAKKQAKNQRRQQKIQERFLHFNEICTTNYTEDNDHEAETALPGEGEVDTEFNRGSQEEL 545
Query: 573 EVFISDNSQQSVSGLSMPVSSLHSEPN--QRPDFSSVNSWNDEAPL--QVQASEVLLLPY 632
+Q+ V+G + S E + P+ SV++ +D L + L +
Sbjct: 546 TQTELCANQKDVNGQEEMIESAADERKCPEHPEVKSVSTESDLGSLTSAPECPRDLNGAF 605
Query: 633 KEESSTAEVAKEDDQASSSILGCAQEDFDGFGLGDMFDEPEIPIGPIGRPSTSNEVAESS 679
EE ++ E+ + + ++ D + + P + + + NE E++
Sbjct: 606 LEERTSGELDITNGLKNLTLNAAVDPDEISIEILNDSHSPALKVYEV-----MNEDPETA 662
HSP 2 Score: 97.4 bits (241), Expect = 9.0e-19
Identity = 54/148 (36.49%), Postives = 77/148 (52.03%), Query Frame = 1
Query: 847 NVKRDATKRFLIHKAPPILTIHMKRFSPDARGRYSKLNGHVRFKETIDLKPYLDTRCA-- 906
+V +A K+ L+ APP+LT+H+KRF A K+N H++F E +DL P+ +C
Sbjct: 677 HVYTNAKKQMLVSLAPPVLTLHLKRFQ-QAGFNLRKVNKHIKFPEILDLAPFCTLKCKNV 736
Query: 907 --DRDKCSYRLVGVVEHSGSMRGGHYVAYVRGGNRK------------RSSGEAEEDASV 966
+ + Y L GVVEHSG+MR GHY AY + E E
Sbjct: 737 AEESTRVLYSLYGVVEHSGTMRSGHYTAYAKERTASCHLSNLVLHGDIPQDCEMESTKGQ 796
Query: 967 WYYASDAVVDEVTLDRVLGCEAYILFYE 979
W++ SD V V + +VL +AY+LFYE
Sbjct: 797 WFHISDTHVQAVPITKVLNSQAYLLFYE 823
BLAST of Cucsa.256480 vs. Swiss-Prot
Match:
UBP16_MOUSE (Ubiquitin carboxyl-terminal hydrolase 16 OS=Mus musculus GN=Usp16 PE=1 SV=2)
HSP 1 Score: 179.5 bits (454), Expect = 1.8e-43
Identity = 174/713 (24.40%), Postives = 287/713 (40.25%), Query Frame = 1
Query: 33 TTVETADEEISIVKEKSQCGHLDKCFNLNELSSKLGSAEPISCEDCQDSSADRRGGRGKA 92
T +A + ++ + C HL K L L + E C+DC+ +
Sbjct: 6 TKGRSAPDTVASESAEPVCRHLRKGLEQGNLKKALVNVEWNICQDCKTDN---------- 65
Query: 93 RHGKKKGGTSVDVKTDAKAIWICLQCGHYACGGIGLPTNSQSHAVRHVRQTR---HPVVI 152
K + + + ++W+CL+CGH CG + + HA++H R H +V+
Sbjct: 66 -----KVKDKPEEEAEDPSVWLCLKCGHQGCGR----DSQEQHALKHYTTPRSEPHYLVL 125
Query: 153 QFENPQLRWCFSCNTLLPVEKTEENGEQKDSLSNV--VKLIRDRSMESTHVDVENTRYTS 212
+N + WC+ C+ + + G+ D + V+ + + H+++EN +
Sbjct: 126 SLDNWSV-WCYKCDEEVKYCSSNRLGQVVDYVRKQAGVRTSKPAEKNNGHIELENKKLEK 185
Query: 213 SEVTAATKPESSVLSD----LDRRNQYIVKGLINLGNTCFFNSILQNLLAIDMLRD---- 272
+ +S L+ +D +Q VKGL NLGNTCFFN+++QNL +LR+
Sbjct: 186 ESKNEQEREKSENLAKETIPMDSASQITVKGLSNLGNTCFFNAVMQNLSQTPVLRELLKE 245
Query: 273 -----HFVKLE--------------ECVGPLTIALKKIFIEARTESRMKSSINPRSVFGC 332
VK+E E GPLT+A+ + E + K + P+ +F
Sbjct: 246 VKMSGTIVKIEPPDLALTEPLEVNLEPPGPLTLAMSQFLSEM--QENKKRVVTPKELFSQ 305
Query: 333 ISSKAPQFKGYEQHDSHELLRVLLDALSSEE-----------------LTSRKMTNSKEE 392
+ KA +FKGY+Q DS ELLR LLD + +EE ++ N ++
Sbjct: 306 VCKKATRFKGYQQQDSQELLRYLLDGMRAEEHQRVSKGILKAFGNSTEKLDEEVKNKVKD 365
Query: 393 RISGNPTPTFVDEMFGGQISSAVCCKECGHTSTVYEPFLDLSLPVPMKKPLAKKVQPVSR 452
P+FVD +FGG+++S + C EC S V+E FLDLSLPV + K +
Sbjct: 366 YEKKKAIPSFVDRIFGGELTSTIMCDECRTVSLVHESFLDLSLPVLDDQSGKKSI----- 425
Query: 453 AKKTKVPPKRNGKTIPKTNKVSDIVPIQIASVPSSSNESSLPSEASASSTTTIMEKTSTS 512
N K + T + D + + S LP S T+ ++K +
Sbjct: 426 ----------NDKNVKMTMEEEDKDSEEEKDDSYMKSRSDLP-----SGTSKHLQKKAKK 485
Query: 513 QNVSDAKESGKEISVEN---------GGECASDNLTWMD-FLEPELNADNCDISTTQDSE 572
Q AK ++ ++ + DN D L E+ D +Q+
Sbjct: 486 QAKKQAKNQRRQQKIQERFLHFNELCATDYTEDNEREADTALAGEVEVDTDSTHGSQEEA 545
Query: 573 NNIEVFISDNSQQSVSGLSMPVSSLHSEPNQRPDFSSVNSWNDEAPLQVQASEVLLLPYK 632
IE+ + +Q+ + G + + + P+ V S N E+ L + V P
Sbjct: 546 TQIELSV---NQKDLDGQESMIERT-PDVQESPEDLGVKSANTESDLGI----VTPAPEC 605
Query: 633 EESSTAEVAKEDDQASSSILGCAQEDFDGFGLGDMFDEPEIPIGPIGRPSTS-------- 679
+E I+ L D EI I I S S
Sbjct: 606 PRDFNGAFLEERTSGELDII----NGLKNLNLNAAVDPDEINI-EIPNDSHSAPKVYEVM 661
HSP 2 Score: 99.0 bits (245), Expect = 3.1e-19
Identity = 67/213 (31.46%), Postives = 99/213 (46.48%), Query Frame = 1
Query: 799 NSDDKSS-CSLPNDEPAKTNIEHLSSHLAVGNQSEKSEDGEM---------DSDSTIVNV 858
N D +++ C+L N E T+ + L ++EK +D + N+
Sbjct: 611 NEDPETAFCTLANREAFSTDECSIQHCLYQFTRNEKLQDANKLLCEVCSRRQCNGPKANI 670
Query: 859 KRD-------ATKRFLIHKAPPILTIHMKRFSPDARGRYSKLNGHVRFKETIDLKPYLDT 918
K D A K+ L+ APP+LT+H+KRF A K+N H++F E +DL P+
Sbjct: 671 KGDRRHVYTNAKKQMLVSLAPPVLTLHLKRFQ-QAGFNLRKVNKHIKFPEILDLAPFCTL 730
Query: 919 RCA----DRDKCSYRLVGVVEHSGSMRGGHYVAYVRGGNRK------------RSSGEAE 978
+C + + Y L GVVEHSG+MR GHY AY + E E
Sbjct: 731 KCKNVAEESTRVLYSLYGVVEHSGTMRSGHYTAYAKERTASCHLSNLVLHGDIPQDCEME 790
BLAST of Cucsa.256480 vs. Swiss-Prot
Match:
UBP16_MACFA (Ubiquitin carboxyl-terminal hydrolase 16 OS=Macaca fascicularis GN=USP16 PE=2 SV=2)
HSP 1 Score: 178.3 bits (451), Expect = 4.0e-43
Identity = 172/694 (24.78%), Postives = 300/694 (43.23%), Query Frame = 1
Query: 51 CGHLDKCFNLNELSSKLGSAEPISCEDCQDSSADRRGGRGKARHGKKKGGTSVDVKTDAK 110
C H+ K L L + E C+DC+ + K + +T+ K
Sbjct: 24 CRHIRKGLEQGNLKKALVNVEWNICQDCKTDN---------------KVKDKAEEETEEK 83
Query: 111 -AIWICLQCGHYACGGIGLPTNSQSHAVRHV---RQTRHPVVIQFENPQLRWCFSCNTLL 170
++W+CL+CGH CG + + HA++H R H +V+ +N + WC+ C+ +
Sbjct: 84 PSVWLCLKCGHQGCGR----NSQEQHALKHYLTPRSEPHCLVLSLDNWSV-WCYICDNEV 143
Query: 171 PVEKTEENGEQKDSLSNVVKLIRDRSMESTH--VDVENTRYTSSEVTAATKPESSVLSD- 230
+ + G+ D + + + E + +++EN + + + ++
Sbjct: 144 QYCSSNQLGQVVDYVRKQASITTPKPAEKDNGNIELENKKLEKESKNEQEREKKENMAKE 203
Query: 231 ---LDRRNQYIVKGLINLGNTCFFNSILQNLLAIDMLRD---------HFVKLE------ 290
++ +Q VKGL NLGNTCFFN+++QNL +LR+ VK+E
Sbjct: 204 NPPMNSPSQITVKGLSNLGNTCFFNAVMQNLSQTPVLRELLKEVKMSGTIVKIEPPDLAL 263
Query: 291 --------ECVGPLTIALKKIFIEARTESRMKSSINPRSVFGCISSKAPQFKGYEQHDSH 350
E GPLT+A+ + F+ E++ K + P+ +F + KA +FKGY+Q DS
Sbjct: 264 TEPLEINLEPPGPLTLAMSQ-FLNEMQETK-KGIVTPKELFSQVCKKAVRFKGYQQQDSQ 323
Query: 351 ELLRVLLDALSSEE-----------------LTSRKMTNSKEERISGNPTPTFVDEMFGG 410
ELLR LLD + +EE ++ N ++ P+FVD +FGG
Sbjct: 324 ELLRYLLDGMRAEEHQRVSKGILKAFGNSTEKLDEELKNKVKDYEKKKSIPSFVDRIFGG 383
Query: 411 QISSAVCCKECGHTSTVYEPFLDLSLPVPMKKPLAKKVQPVSRAKKTKVPPKRNGKTIPK 470
+++S + C +C S V+E FLDLSLPV + K V N K + K
Sbjct: 384 ELTSTIMCDQCRTVSLVHESFLDLSLPVLDDQSGKKSV---------------NDKNLKK 443
Query: 471 TNKVSDIVPIQIASVPSSSNESSLPSEAS-ASSTTTIMEKTSTSQNVSDAKESGKEISVE 530
T + D S N+S + + S T+ ++K + Q AK ++ ++
Sbjct: 444 TMEDED-----QDSEEEKDNDSYIKERSDIPSGTSKHLQKKAKKQAKKQAKNQRRQQKIQ 503
Query: 531 N-----GGECASDNLTWMDF-----LEPELNADNCDISTTQDSENNIEVFISDNSQQSVS 590
C D+ ++ L+ E+N + IS Q+ + E + +Q+ ++
Sbjct: 504 GKVLHLNDICTIDHPEDNEYEAEMSLQGEVNIKSNHIS--QEGVMHKEYCV---NQKDLN 563
Query: 591 GLSMPVSSL--HSEPNQRPDFSSVNSWNDEAPLQVQASEV---LLLPYKEESSTAEVAKE 650
G + S+ + +P + D ++N ND L +E L Y E+S EV
Sbjct: 564 GQEKMIESVTDNQKPTEEVDMKNINMDNDLEVLTSSPTECTRNLNGAYLTEASNGEVDIS 623
Query: 651 DDQASSSILGCAQEDFDGFGLGDMFDEPEIPIGPIGRPSTSNEVAESSFNSESDPEEVDN 679
+ + ++ D + + P + + NE E++F + ++ EV N
Sbjct: 624 NGFKNLNLNAALHPDEINIEILNDSHTPGTKVYEV-----VNEDPETAFCTLAN-REVFN 663
HSP 2 Score: 99.0 bits (245), Expect = 3.1e-19
Identity = 58/147 (39.46%), Postives = 82/147 (55.78%), Query Frame = 1
Query: 847 NVKRDATKRFLIHKAPPILTIHMKRFSPDARGRYSKLNGHVRFKETIDLKPYLDTRCA-- 906
+V +A K+ LI APP+LT+H+KRF A K+N H++F E +DL P+ +C
Sbjct: 678 HVYTNAKKQMLISLAPPVLTLHLKRFQ-QAGFNLRKVNKHIKFPEILDLAPFCTLKCKNV 737
Query: 907 --DRDKCSYRLVGVVEHSGSMRGGHYVAY--VRGGNRKRSS----GEAEEDASV-----W 966
+ + Y L GVVEHSG+MR GHY AY R N S+ G+ +D + W
Sbjct: 738 AEENTRVLYSLYGVVEHSGTMRSGHYTAYAKARTANSHLSNLVLHGDIPQDFEMESKGQW 797
Query: 967 YYASDAVVDEVTLDRVLGCEAYILFYE 979
++ SD V V +VL +AY+LFYE
Sbjct: 798 FHISDTHVQAVPTTKVLNSQAYLLFYE 823
BLAST of Cucsa.256480 vs. TrEMBL
Match:
A0A0A0L9E4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G130860 PE=3 SV=1)
HSP 1 Score: 1967.2 bits (5095), Expect = 0.0e+00
Identity = 980/980 (100.00%), Postives = 980/980 (100.00%), Query Frame = 1
Query: 1 MGKKVKKKTNRAPPKEKRVSSSSPRKVPQPSNTTVETADEEISIVKEKSQCGHLDKCFNL 60
MGKKVKKKTNRAPPKEKRVSSSSPRKVPQPSNTTVETADEEISIVKEKSQCGHLDKCFNL
Sbjct: 1 MGKKVKKKTNRAPPKEKRVSSSSPRKVPQPSNTTVETADEEISIVKEKSQCGHLDKCFNL 60
Query: 61 NELSSKLGSAEPISCEDCQDSSADRRGGRGKARHGKKKGGTSVDVKTDAKAIWICLQCGH 120
NELSSKLGSAEPISCEDCQDSSADRRGGRGKARHGKKKGGTSVDVKTDAKAIWICLQCGH
Sbjct: 61 NELSSKLGSAEPISCEDCQDSSADRRGGRGKARHGKKKGGTSVDVKTDAKAIWICLQCGH 120
Query: 121 YACGGIGLPTNSQSHAVRHVRQTRHPVVIQFENPQLRWCFSCNTLLPVEKTEENGEQKDS 180
YACGGIGLPTNSQSHAVRHVRQTRHPVVIQFENPQLRWCFSCNTLLPVEKTEENGEQKDS
Sbjct: 121 YACGGIGLPTNSQSHAVRHVRQTRHPVVIQFENPQLRWCFSCNTLLPVEKTEENGEQKDS 180
Query: 181 LSNVVKLIRDRSMESTHVDVENTRYTSSEVTAATKPESSVLSDLDRRNQYIVKGLINLGN 240
LSNVVKLIRDRSMESTHVDVENTRYTSSEVTAATKPESSVLSDLDRRNQYIVKGLINLGN
Sbjct: 181 LSNVVKLIRDRSMESTHVDVENTRYTSSEVTAATKPESSVLSDLDRRNQYIVKGLINLGN 240
Query: 241 TCFFNSILQNLLAIDMLRDHFVKLEECVGPLTIALKKIFIEARTESRMKSSINPRSVFGC 300
TCFFNSILQNLLAIDMLRDHFVKLEECVGPLTIALKKIFIEARTESRMKSSINPRSVFGC
Sbjct: 241 TCFFNSILQNLLAIDMLRDHFVKLEECVGPLTIALKKIFIEARTESRMKSSINPRSVFGC 300
Query: 301 ISSKAPQFKGYEQHDSHELLRVLLDALSSEELTSRKMTNSKEERISGNPTPTFVDEMFGG 360
ISSKAPQFKGYEQHDSHELLRVLLDALSSEELTSRKMTNSKEERISGNPTPTFVDEMFGG
Sbjct: 301 ISSKAPQFKGYEQHDSHELLRVLLDALSSEELTSRKMTNSKEERISGNPTPTFVDEMFGG 360
Query: 361 QISSAVCCKECGHTSTVYEPFLDLSLPVPMKKPLAKKVQPVSRAKKTKVPPKRNGKTIPK 420
QISSAVCCKECGHTSTVYEPFLDLSLPVPMKKPLAKKVQPVSRAKKTKVPPKRNGKTIPK
Sbjct: 361 QISSAVCCKECGHTSTVYEPFLDLSLPVPMKKPLAKKVQPVSRAKKTKVPPKRNGKTIPK 420
Query: 421 TNKVSDIVPIQIASVPSSSNESSLPSEASASSTTTIMEKTSTSQNVSDAKESGKEISVEN 480
TNKVSDIVPIQIASVPSSSNESSLPSEASASSTTTIMEKTSTSQNVSDAKESGKEISVEN
Sbjct: 421 TNKVSDIVPIQIASVPSSSNESSLPSEASASSTTTIMEKTSTSQNVSDAKESGKEISVEN 480
Query: 481 GGECASDNLTWMDFLEPELNADNCDISTTQDSENNIEVFISDNSQQSVSGLSMPVSSLHS 540
GGECASDNLTWMDFLEPELNADNCDISTTQDSENNIEVFISDNSQQSVSGLSMPVSSLHS
Sbjct: 481 GGECASDNLTWMDFLEPELNADNCDISTTQDSENNIEVFISDNSQQSVSGLSMPVSSLHS 540
Query: 541 EPNQRPDFSSVNSWNDEAPLQVQASEVLLLPYKEESSTAEVAKEDDQASSSILGCAQEDF 600
EPNQRPDFSSVNSWNDEAPLQVQASEVLLLPYKEESSTAEVAKEDDQASSSILGCAQEDF
Sbjct: 541 EPNQRPDFSSVNSWNDEAPLQVQASEVLLLPYKEESSTAEVAKEDDQASSSILGCAQEDF 600
Query: 601 DGFGLGDMFDEPEIPIGPIGRPSTSNEVAESSFNSESDPEEVDNTDAPVSVESCLTFFTK 660
DGFGLGDMFDEPEIPIGPIGRPSTSNEVAESSFNSESDPEEVDNTDAPVSVESCLTFFTK
Sbjct: 601 DGFGLGDMFDEPEIPIGPIGRPSTSNEVAESSFNSESDPEEVDNTDAPVSVESCLTFFTK 660
Query: 661 PELLSNENGYNCEKCSKRLQQQRLEMKKQSKVACKAVANGCQTAVGGDISSCNEDSSVEV 720
PELLSNENGYNCEKCSKRLQQQRLEMKKQSKVACKAVANGCQTAVGGDISSCNEDSSVEV
Sbjct: 661 PELLSNENGYNCEKCSKRLQQQRLEMKKQSKVACKAVANGCQTAVGGDISSCNEDSSVEV 720
Query: 721 KNKRNMNLTNGSISYSSGESSNLKKNVDCSSQDCSKPVNCQKSKTDPPVLDEDEAKVDKD 780
KNKRNMNLTNGSISYSSGESSNLKKNVDCSSQDCSKPVNCQKSKTDPPVLDEDEAKVDKD
Sbjct: 721 KNKRNMNLTNGSISYSSGESSNLKKNVDCSSQDCSKPVNCQKSKTDPPVLDEDEAKVDKD 780
Query: 781 MNPGLSRSSGCNNTRNQENSDDKSSCSLPNDEPAKTNIEHLSSHLAVGNQSEKSEDGEMD 840
MNPGLSRSSGCNNTRNQENSDDKSSCSLPNDEPAKTNIEHLSSHLAVGNQSEKSEDGEMD
Sbjct: 781 MNPGLSRSSGCNNTRNQENSDDKSSCSLPNDEPAKTNIEHLSSHLAVGNQSEKSEDGEMD 840
Query: 841 SDSTIVNVKRDATKRFLIHKAPPILTIHMKRFSPDARGRYSKLNGHVRFKETIDLKPYLD 900
SDSTIVNVKRDATKRFLIHKAPPILTIHMKRFSPDARGRYSKLNGHVRFKETIDLKPYLD
Sbjct: 841 SDSTIVNVKRDATKRFLIHKAPPILTIHMKRFSPDARGRYSKLNGHVRFKETIDLKPYLD 900
Query: 901 TRCADRDKCSYRLVGVVEHSGSMRGGHYVAYVRGGNRKRSSGEAEEDASVWYYASDAVVD 960
TRCADRDKCSYRLVGVVEHSGSMRGGHYVAYVRGGNRKRSSGEAEEDASVWYYASDAVVD
Sbjct: 901 TRCADRDKCSYRLVGVVEHSGSMRGGHYVAYVRGGNRKRSSGEAEEDASVWYYASDAVVD 960
Query: 961 EVTLDRVLGCEAYILFYEIT 981
EVTLDRVLGCEAYILFYEIT
Sbjct: 961 EVTLDRVLGCEAYILFYEIT 980
BLAST of Cucsa.256480 vs. TrEMBL
Match:
A0A067L5H9_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_01121 PE=3 SV=1)
HSP 1 Score: 938.7 bits (2425), Expect = 5.6e-270
Identity = 530/1023 (51.81%), Postives = 676/1023 (66.08%), Query Frame = 1
Query: 1 MGKKVKKKTNRAPPKEKRVSSSSPRKVPQPSNTTVETADEEISIVKEKSQCGHLDKCFNL 60
MGK+VKKK+ R+ KEKR++ SP+ P SN +DE ++ +KE+ C HLDK FNL
Sbjct: 1 MGKRVKKKS-RSLQKEKRITPHSPKIAPLQSNPGDNISDEGVAALKERKSCAHLDKGFNL 60
Query: 61 NELSSKLGSAEPISCEDCQDSSADRRGGRGKARHGKKKGGTSVDVKTDAKAIWICLQCGH 120
N LS K GS+ P+ CEDC++ ADRRG +GK +HGKKKG S D K+++KAIW+CL+CGH
Sbjct: 61 NNLSEKFGSSNPLRCEDCREGVADRRGAKGKGKHGKKKG--SADPKSESKAIWVCLECGH 120
Query: 121 YACGGIGLPTNSQSHAVRHVRQTRHPVVIQFENPQLRWCFSCNTLLPVEKTEENGEQKDS 180
YACGG+GLPT+ QSHA+RH RQTRHP+V+Q+ENP LRWCF CNTL+PVE EE+GE+KD+
Sbjct: 121 YACGGVGLPTSPQSHALRHNRQTRHPLVMQWENPHLRWCFLCNTLIPVETMEESGEKKDA 180
Query: 181 LSNVVKLIRDRSMESTHVDVENTRYTSSEVTAATKPESSVLSDLDRRNQYIVKGLINLGN 240
L +VVKL++ RS E + VDVE+ + V + K E +V + L+ R YIV+GL+NLGN
Sbjct: 181 LLDVVKLMKTRSSERSSVDVEDVWFGGGSVASEIKAEGTVSNSLEGRGGYIVRGLVNLGN 240
Query: 241 TCFFNSILQNLLAIDMLRDHFVKLEECVGPLTIALKKIFIEARTESRMKSSINPRSVFGC 300
TCFFNS++QNLL++D LRD F + GPLTIALKK+F E + E+ +KS INPRS FGC
Sbjct: 241 TCFFNSVMQNLLSMDKLRDFFFDQDASFGPLTIALKKLFAETKPETGLKSVINPRSFFGC 300
Query: 301 ISSKAPQFKGYEQHDSHELLRVLLDALSSEELTSRKMTN-SKEERISGNPTPTFVDEMFG 360
I SKAPQF+GY+Q DSHELLR LLD LSSEEL RK N SKE+ IS TFVD +FG
Sbjct: 301 ICSKAPQFRGYQQQDSHELLRCLLDGLSSEELGVRKQINASKEDGISLKHGSTFVDALFG 360
Query: 361 GQISSAVCCKECGHTSTVYEPFLDLSLPVPMKKPLAKKVQPVSRAKKTKVPPKRNGKTIP 420
G+ISS V C ECGH+STVYEPFLDLSLPVP KKP K+ Q V+R KKTK+PPKR GK
Sbjct: 361 GRISSTVSCIECGHSSTVYEPFLDLSLPVPTKKPPTKRAQLVARPKKTKLPPKRGGKIRG 420
Query: 421 KTNKVSDIVPIQIASVPSSSNESSLPS--------EASASS----------TTTIMEKTS 480
K NK D VP Q S P++S ES + +A ASS T++ +K S
Sbjct: 421 KANKDIDSVPAQSISNPTASKESPCQTLSMVAQAEKAVASSGDAAGSNSVCLTSMDDKGS 480
Query: 481 -TSQNVSDAKESGKEISVENGGE---CASDNLTWMDFL-------EPELNADNCDISTTQ 540
SQ +S A ++ + VE E + DN TWMD+L +P L + D+ST+Q
Sbjct: 481 LVSQKISTAPDADNDQVVEAKVEQTAASFDNFTWMDYLGQETIADDPYLTSQKNDVSTSQ 540
Query: 541 DSENNIEVFISDNSQQSVSGLSMPVSSLHSEPNQRPDFSSVNSWNDEAPLQVQASEVLLL 600
S+N I +D +S V S+ EP + D SSVN W +E PLQV +SEVLLL
Sbjct: 541 YSKNMIP---NDGLMESSH-----VFSVDGEPTPKLDSSSVNPWEEEVPLQVTSSEVLLL 600
Query: 601 PYKEESST-AEVAKEDDQASSSILGCAQE-DFDGFGLGDMFDEPEIPIGPIGRPSTSNEV 660
P KEES T E+ + + SSS++G E DFDGFG D+F+EPE+ GP+ PS NE
Sbjct: 601 PCKEESFTNGEIIRGEAGPSSSVVGGQDEADFDGFG--DLFNEPEVSSGPVAGPSLDNET 660
Query: 661 AESSF----NSESDPEEVDNTDAPVSVESCLTFFTKPELLSNENGYNCEKCSKRLQQQRL 720
A + F +S+SDP+EVDN+D+PVS+ESCL F KPELLSN+N + CE CSK L ++RL
Sbjct: 661 ARTGFTAGNSSDSDPDEVDNSDSPVSIESCLAHFIKPELLSNDNAWECENCSKTLPRRRL 720
Query: 721 EMKK-QSKVACKAVANGCQTAVGGDISSCNEDS--SVEVKNKRNMNLTNGSISYSSGESS 780
E KK QSK A + + NG +T + + ++D+ S EV+N +N ++ +I +GES
Sbjct: 721 ETKKKQSKTALETMINGGETRFHSHLPNLDKDNLHSTEVRNLQNGDMNTANILNFTGESL 780
Query: 781 NLKKNVDCSSQDCSKPVNCQKSKTDPPVLDEDEAKVDKDMNPG---LSRSSGCNNTRNQE 840
N K V CSSQ+C K + S+TD L +++ DM SRSS + +QE
Sbjct: 781 NNGKIV-CSSQNCIK---AETSQTDELKLSVSQSEQKGDMTVAHAEQSRSSTLYESCSQE 840
Query: 841 NSD---DKSSCSLPNDEPAKTNIEHLSSHLAVGNQSEKSEDGEMDSDSTIVNVKRDATKR 900
+ D S+ P+ T + L GN + ED + + S V VKRDATKR
Sbjct: 841 SISSAVDSSTVDEPSSTGYTTAKDQLDDSQLSGN-CDAEEDEDKERTSKKVKVKRDATKR 900
Query: 901 FLIHKAPPILTIHMKRFSPDARGRYSKLNGHVRFKETIDLKPYLDTRCADRDKCSYRLVG 960
L+ KAPP+LTIH+KRFS DARGR SKLNGHV F E +DL+PYLD RC DR+K YRLVG
Sbjct: 901 VLVDKAPPVLTIHLKRFSQDARGRLSKLNGHVNFGEILDLRPYLDPRCVDREKYMYRLVG 960
Query: 961 VVEHSGSMRGGHYVAYVRGGNRKRSSGEAEEDASVWYYASDAVVDEVTLDRVLGCEAYIL 979
VVEH G+M GGHYVAYVRGG R + E E SVWY+ASDA V EV+L+ VL CEAYIL
Sbjct: 961 VVEHLGTMIGGHYVAYVRGGVRSKGKEENENGDSVWYHASDAYVREVSLEEVLRCEAYIL 1005
BLAST of Cucsa.256480 vs. TrEMBL
Match:
B9RWG7_RICCO (Putative uncharacterized protein OS=Ricinus communis GN=RCOM_1019320 PE=3 SV=1)
HSP 1 Score: 913.7 bits (2360), Expect = 1.9e-262
Identity = 508/1020 (49.80%), Postives = 666/1020 (65.29%), Query Frame = 1
Query: 1 MGKKVKKKTNRAPPKEKRVSSSSPRKVPQPSNTTVETADE-EISIVKEKSQCGHLDKCFN 60
MGK+VKKK+ R+ KEKRVS+ SP+ PQ + +V+ + +++VKE+ QC HL K FN
Sbjct: 1 MGKRVKKKS-RSLQKEKRVSTHSPKVAPQQTTPSVDVNERITVAVVKERKQCVHLGKGFN 60
Query: 61 LNELSSKLGSAEPISCEDCQDSSADRRGGRGKARHGKKKGGTSVDVKTDAKAIWICLQCG 120
LN L+ KLGS++P+ CEDC++ ADRRG +GK +HGKKKG SVD K+++KAIW+CL+CG
Sbjct: 61 LNNLTVKLGSSDPLKCEDCREGVADRRGAKGKGKHGKKKG--SVDSKSESKAIWVCLECG 120
Query: 121 HYACGGIGLPTNSQSHAVRHVRQTRHPVVIQFENPQLRWCFSCNTLLPVEKTEENGEQKD 180
H+ACGG+GLPT QSH VRH RQTRHP++IQ+ENP LRWCF CNTL+PVE TEENGE+KD
Sbjct: 121 HFACGGVGLPTTPQSHVVRHARQTRHPLIIQWENPHLRWCFPCNTLIPVEMTEENGEKKD 180
Query: 181 SLSNVVKLIRDRSMESTHVDVENTRYTSSEVTAATKPESSVLSDLDRRNQYIVKGLINLG 240
+L +VV LI+ RS + + VDVE+ + V + K E ++LS + ++ Y V+GL+NLG
Sbjct: 181 ALLDVVNLIKTRSSQRSLVDVEDVWFGGGSVASEIKAEGTILSSTEGKSGYTVRGLVNLG 240
Query: 241 NTCFFNSILQNLLAIDMLRDHFVKLEECVGPLTIALKKIFIEARTESRMKSSINPRSVFG 300
NTCFFNS++QNLLAID LRD F + GPLTIALKK+F E + E+ +K+ I+PRS FG
Sbjct: 241 NTCFFNSVMQNLLAIDKLRDFFFNQDISFGPLTIALKKLFTETKQETGIKNVISPRSFFG 300
Query: 301 CISSKAPQFKGYEQHDSHELLRVLLDALSSEELTSRKMTN-SKEERISGNPTPTFVDEMF 360
I SKAPQF+GY+Q DSHELLR LLD LSSEEL RK N SKE IS PTFVD +F
Sbjct: 301 SICSKAPQFRGYQQQDSHELLRYLLDGLSSEELAVRKQVNASKENGISSKHGPTFVDVLF 360
Query: 361 GGQISSAVCCKECGHTSTVYEPFLDLSLPVPMKKPLAKKVQPVSRAKKTKVPPKRNGKTI 420
GG+I S V C EC ++STVYEPFLDLSLPVP KKP KK QP SR+KKTK+P KR G+
Sbjct: 361 GGRICSTVSCIECEYSSTVYEPFLDLSLPVPTKKPATKKAQPASRSKKTKLPLKRGGRVR 420
Query: 421 PKTNKVSDIVPIQIASVPSSSNESS------LP-SEASASST-----------TTIMEKT 480
K NK +D VP Q +S PS S+ES +P +E S +S+ TT+ +K+
Sbjct: 421 AKANKDTDAVPAQSSSNPSVSSESPCQTLSIIPHAENSMASSGDVVGLESVCLTTVADKS 480
Query: 481 S-TSQNVSDAKESGKEISVENGGECAS---DNLTWMDFL-------EPELNADNCDISTT 540
SQN S ++ E E E + D+ +WMD+L E +L N D ST+
Sbjct: 481 GLASQNFSTIPDTENEQVTEGTVEQTTNSFDDFSWMDYLGQETVTDEHDLTLQNKDASTS 540
Query: 541 QDSENNIEVFISDNSQQSVSGLSMPVSSLHSEPNQRPDFSSVNSWNDEAPLQVQASEVLL 600
Q SEN + +D+ + S VS + EPN + + SSVN W +E QV++SEVLL
Sbjct: 541 QFSEN---IIPNDDIME-----SSQVSPVDGEPNLKLE-SSVNPWEEEVLAQVKSSEVLL 600
Query: 601 LPYKEESSTAEVAKEDDQASSSILGCAQEDFDGFGLGDMFDEPEIPIGPIGRPSTSNEVA 660
LPYKEES + +SS++GC Q++ D G GD+F+EPE+ GP+ PS +N A
Sbjct: 601 LPYKEESVMDGDVMKGQAEASSVVGCGQDEADFDGFGDLFNEPEVSSGPVSGPSLANGTA 660
Query: 661 ESSF----NSESDPEEVDNTDAPVSVESCLTFFTKPELLSNENGYNCEKCSKRLQQQRLE 720
E+ F NSESDP+EVDN+D+PVS+ESCL F KPELLSN+N + CE CSK LQ+QRLE
Sbjct: 661 ETGFIAANNSESDPDEVDNSDSPVSIESCLAHFIKPELLSNDNAWECENCSKTLQRQRLE 720
Query: 721 MKKQSKVACKAVANGCQTAVGGDISSCNEDS--SVEVKNKRNMNLTNGSISYSSGESSNL 780
KK++K + + G + + SS +D+ S+EVK+ T+ + S +
Sbjct: 721 AKKKAKTTVETMIIGGKAQIQSP-SSLEKDNLCSIEVKDHNGGINTDTCFNSSGASLVSD 780
Query: 781 KKNVDCSSQDCSKPVNCQKSKTDPPVLDEDEAKVDKD---MNPGLSRSS--GCNNTRNQE 840
+N+D ++Q+ K + Q + +P DE K + M LS S+ C+
Sbjct: 781 DENIDRTNQNYIKTESGQTDELNPIETQGDEQKGEMTVALMEQSLSSSTYKSCSQESFSC 840
Query: 841 NSDDKSSCSLPNDEPAKTNIEHLSSHLAVGNQSEKSEDGEMDSDSTIVNVKRDATKRFLI 900
SS P+ T + + GN K ++ + S V VKRDATKR L+
Sbjct: 841 PVVGSSSVGEPSSTGYATAKDQMGDSQFSGNCGAKEDE---EGTSRKVKVKRDATKRVLV 900
Query: 901 HKAPPILTIHMKRFSPDARGRYSKLNGHVRFKETIDLKPYLDTRCADRDKCSYRLVGVVE 960
KAPPILTIH+KRFS DARGR SKLNGHV F + +DL+PY+D RC DR+K YRL+GVVE
Sbjct: 901 DKAPPILTIHLKRFSQDARGRLSKLNGHVNFGDVLDLRPYMDPRCTDREKYVYRLLGVVE 960
Query: 961 HSGSMRGGHYVAYVRGGNRKRSSGEAEEDASVWYYASDAVVDEVTLDRVLGCEAYILFYE 979
H G+MRGGHYVAYVRGG + + E E +SVWY+ASDA V EV+L+ VL CEAYILFYE
Sbjct: 961 HLGTMRGGHYVAYVRGGQKSKGKAENESGSSVWYHASDAYVREVSLEEVLRCEAYILFYE 1004
BLAST of Cucsa.256480 vs. TrEMBL
Match:
A0A061EJT6_THECC (Ubiquitin carboxyl-terminal hydrolase, putative isoform 1 OS=Theobroma cacao GN=TCM_020316 PE=3 SV=1)
HSP 1 Score: 910.6 bits (2352), Expect = 1.6e-261
Identity = 516/1043 (49.47%), Postives = 674/1043 (64.62%), Query Frame = 1
Query: 1 MGKKVKKKTNRAPPKEKRV-SSSSPRKVPQPSNTTVETADEEISIVKEKSQCGHLDKCFN 60
MGK+VKK R PPKEK+V ++ SP+ +PQ +N + E D+ +++VKE+ C HLDK
Sbjct: 1 MGKRVKKN-RRVPPKEKKVVAAQSPKVIPQENNASFEKVDDGVAVVKERKSCPHLDKGIY 60
Query: 61 LNELSSKLGSAEPISCEDCQDSSADRRGGRGKARHGKKKGGTSVDVKTDAKAIWICLQCG 120
L++L +KL S+ PI CEDC++ DRRG +GK +HGKKKG SVD K+++KAIW+CL+CG
Sbjct: 61 LDKLLAKLRSSGPIRCEDCREGGNDRRGSKGKGKHGKKKGSASVDSKSESKAIWVCLECG 120
Query: 121 HYACGGIGLPTNSQSHAVRHVRQTRHPVVIQFENPQLRWCFSCNTLLPVEKTEENGEQKD 180
H+ C G+GLPT S +HA+RH+RQTRH ++IQ++NPQLRWCF+C+T +PVEKTEEN E KD
Sbjct: 121 HFVCAGVGLPTASTTHAIRHIRQTRHHLMIQWDNPQLRWCFACSTFIPVEKTEENVENKD 180
Query: 181 SLSNVVKLIRDRSMESTHVDVENTRYTSSEVTAATKPESSVLSDLDRRNQYIVKGLINLG 240
+LS VVKLI++RS E DVEN + S VT A K E ++ + LD ++ Y+V+GL+NLG
Sbjct: 181 ALSEVVKLIKERSSEPPAADVENVWFGSGSVTNAIKSEGTISNGLDEKSGYMVRGLVNLG 240
Query: 241 NTCFFNSILQNLLAIDMLRDHFVKLEECVGPLTIALKKIFIEARTESRMKSSINPRSVFG 300
NTCFFNS++QNLLA+D LRD+F+ L+ G LTI+LKK+F E + E +K++INP+ FG
Sbjct: 241 NTCFFNSVMQNLLALDRLRDYFLNLDASGGQLTISLKKLFAETKPEMGLKNAINPKPFFG 300
Query: 301 CISSKAPQFKGYEQHDSHELLRVLLDALSSEELTSRKMTN-SKEERISGNPTPTFVDEMF 360
CI +KAPQF+GY+QHDSHELLR LLD L +EEL +K N S + +S N TFVD +F
Sbjct: 301 CICAKAPQFRGYQQHDSHELLRCLLDGLYTEELALKKHINASINDVVSANQDLTFVDAVF 360
Query: 361 GGQISSAVCCKECGHTSTVYEPFLDLSLPVPMKKPLAKKVQPVSRAKKTKVPPKRNGKTI 420
GGQISS +CC+ECGH+STVYEPFLDLSLPVP KK +KK QPVSRAKKTK+PPK+ G+
Sbjct: 361 GGQISSTLCCEECGHSSTVYEPFLDLSLPVPTKKTPSKKAQPVSRAKKTKLPPKKVGRAR 420
Query: 421 PKTNKVSDIVPIQ--IASVPSS------------------SNESSLPSEASASSTTTIME 480
K NK D P Q S+PSS S+ SL S A +S E
Sbjct: 421 GKVNKDVDRSPAQGVTTSLPSSESPGLGHMVVPQTETMVASSSDSLLSGAVGTSAEA-NE 480
Query: 481 KTSTSQNVSDAKESGKEISVENG-----GEC--------------------ASDNLTWMD 540
+S SQN+ S E +EN G +D TWMD
Sbjct: 481 LSSASQNLLAVAASENEQVMENAVKENTGAADDFAWMDYLVMENTLQENAAGADGFTWMD 540
Query: 541 FLEP-------ELNADNCDISTTQDSENNIEVFISDNSQQSVSGLSMPVSSLHSEPNQRP 600
+LEP +L + N DIS QDSE+ V ++ S VS L EPN +P
Sbjct: 541 YLEPGTIAVENDLISQNNDISFFQDSEDKNLVLNEALAESS------QVSLLEGEPNWKP 600
Query: 601 DFSSVNSWNDEAPLQVQASEVLLLPYKEESSTAEVAKEDDQASSSILGCAQEDFDGFGLG 660
SS N +E PL VQ SEVLLLPYKEES++++ + +++ASSS +G QE+ + G G
Sbjct: 601 HDSSGNLQEEELPLLVQDSEVLLLPYKEESTSSKESVRENEASSSNVGHGQEEVEFDGFG 660
Query: 661 DMFDEPEIPIGPIGRPSTSNEVAESSF----NSESDPEEVDNTDAPVSVESCLTFFTKPE 720
DMF+EPEI GP PS +NEVAE+ F S+SDP+EVD++D+PVSVESCL F KPE
Sbjct: 661 DMFNEPEIAEGPSIGPSLANEVAETGFLAGNISDSDPDEVDDSDSPVSVESCLAHFIKPE 720
Query: 721 LLSNENGYNCEKCSKRLQQQRLEMKKQSKVACKAVANGCQTAVGGDISSCNEDSSVEVKN 780
LLS++N +NCE C+K L+ Q+LE KK+ K + NG +T S C S +
Sbjct: 721 LLSDDNAWNCENCAKILRSQKLESKKKQTKMSKNLTNGGETQ-----SQCEPPSLDKEFP 780
Query: 781 KRN--MNLTNGSISYSSGESSNL-KKNVDCSSQDCSKPVNCQKSKTDPPVLDEDEAKVD- 840
N ++NG IS +SGES L K D Q+ K Q + + V +E K +
Sbjct: 781 CPNGVRTISNGDIS-NSGESLVLHNKITDSLKQNGIKLEIGQTGELNSVVSKSEEGKSEI 840
Query: 841 KDMNPGLSRSSGCNNTRNQENS---DDKSSCSLPNDEPAKTNIEHLSSHLAVGNQSEKSE 900
+D + S SS + + QE S SC++ N + +S +A QS +SE
Sbjct: 841 EDASLMKSGSSVSSKSCGQEESGGIQPVDSCNVENHSD-NDKFQQSNSQMAENCQSGESE 900
Query: 901 DGEMDSDSTIVNVKRDATKRFLIHKAPPILTIHMKRFSPDARGRYSKLNGHVRFKETIDL 960
D E+DS + V VKR+ATKR LI+KAPPILTIH+KRFS DARGR SKLNGHV F+ETIDL
Sbjct: 901 DEEIDSKN--VKVKRNATKRVLINKAPPILTIHLKRFSQDARGRLSKLNGHVNFRETIDL 960
Query: 961 KPYLDTRCADRDKCSYRLVGVVEHSGSMRGGHYVAYVRGGNRKRSSGEAEEDASVWYYAS 979
+PY+D RC D D C Y L+GVVEHSG+MRGGHY+AYVRGG +++ E E +S WYY S
Sbjct: 961 RPYVDARCEDIDNCIYHLMGVVEHSGTMRGGHYIAYVRGGEKRKGKAETEYVSSPWYYVS 1020
BLAST of Cucsa.256480 vs. TrEMBL
Match:
A0A0D2RE60_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_005G130100 PE=3 SV=1)
HSP 1 Score: 884.0 bits (2283), Expect = 1.6e-253
Identity = 484/1035 (46.76%), Postives = 664/1035 (64.15%), Query Frame = 1
Query: 1 MGKKVKKKTNRAPPKEKRV-SSSSPRKVPQPSNTTVETADEEISIVKEKSQCGHLDKCFN 60
MGK+VKK R PPKEK+V ++ SP+ +PQ ++ D+E+ VKE+ +C H DK N
Sbjct: 1 MGKRVKKN-RRVPPKEKKVVAAQSPKAIPQEKTAGIDDVDDEVKAVKERKKCLHFDKGIN 60
Query: 61 LNELSSKLGSAEPISCEDCQDSSADRRGGRGKARHGKKKGGTSVDVKTDAKAIWICLQCG 120
+++L +KL S++PI CEDC++ DRRGG+GK++HGKKKG SV+ K+++KAIW+CL+CG
Sbjct: 61 IDKLLTKLRSSDPIRCEDCREGRNDRRGGKGKSKHGKKKGSASVESKSESKAIWVCLECG 120
Query: 121 HYACGGIGLPTNSQSHAVRHVRQTRHPVVIQFENPQLRWCFSCNTLLPVEKTEENGEQKD 180
HY C G+GLPT S SHAVRH+RQTRH +V+Q++NPQLRWCFSCN +PVEK EEN E KD
Sbjct: 121 HYVCAGVGLPTASTSHAVRHLRQTRHHLVVQWDNPQLRWCFSCNIFIPVEKMEENSESKD 180
Query: 181 SLSNVVKLIRDRSMESTHVDVENTRYTSSEVTAATKPESSVLSDLDRRNQYIVKGLINLG 240
L VVKLI++RS ES+ DVE+ S V K E ++ + LD +N Y+V+GL+NLG
Sbjct: 181 VLYEVVKLIKERSSESSTNDVEDVLSGSGSVICDIKSEGTISNFLDGKNGYVVRGLVNLG 240
Query: 241 NTCFFNSILQNLLAIDMLRDHFVKLEECVGPLTIALKKIFIEARTESRMKSSINPRSVFG 300
NTCFFNS++QNLLA++ LRD+F L+ +G LTI++KK+F E R E +K++INP+ F
Sbjct: 241 NTCFFNSVMQNLLALNRLRDYFSNLDVSMGQLTISMKKLFAEIRPEMGLKNAINPKPFFA 300
Query: 301 CISSKAPQFKGYEQHDSHELLRVLLDALSSEELTSRKMTN-SKEERISGNPTPTFVDEMF 360
+ SKAPQF+GY+QHDSHELLR LLD L +EEL +K N SK + ++ + TFVD +F
Sbjct: 301 SLCSKAPQFRGYQQHDSHELLRCLLDGLYTEELALKKHINASKSDAVTASQDLTFVDAVF 360
Query: 361 GGQISSAVCCKECGHTSTVYEPFLDLSLPVPMKKPLAKKVQPVSRAKKTKVPPKRNGKTI 420
GGQISS +CC+ECGH+STVYEPFLDLSL VP KK +KK QPVSRAKKTK+PPK+ G+
Sbjct: 361 GGQISSTLCCEECGHSSTVYEPFLDLSLSVPTKKTPSKKAQPVSRAKKTKLPPKKVGRNR 420
Query: 421 PKTNKVSDIVPIQIASVPSSSNESSLPSEA-------------------SASSTTTIMEK 480
K NK D P ++ + P ++ESS P +A +T E
Sbjct: 421 GKVNKDVDQAPAKVVTAPVPNSESSGPGHIAVPQMEPKVASSGDSSLSHAAGPSTKADES 480
Query: 481 TSTSQNVSDAKESGKEISVEN-------------------------GGECASDNLTWMDF 540
+S S+N+ D ES KE VE+ + +D+ +WMD+
Sbjct: 481 SSASRNLFDVVESQKEQVVESTVKENSAGTDDFAWLDYLTTENTLPENDTGADDFSWMDY 540
Query: 541 LEPELNADNCDISTTQDSENNIEVFISDNSQ---QSVSGLSMPVSSLHSEPNQRPDFSSV 600
L+ E+ AD D+ + NN + S+ + S VS L E N++ + SS
Sbjct: 541 LQQEIVADESDLIS---QNNNTSLQDSEEKELVPNEALAESCEVSVLEGETNKKTEDSSG 600
Query: 601 NSWNDEAPLQVQASEVLLLPYKEESSTAEVAKEDDQASSSILGCAQEDFDGFGLGDMFDE 660
N ++ PL VQ S VLLLPYKE+ ++ +++ASSS G QE+ + G GDMF+E
Sbjct: 601 NLQEEDLPLLVQDSVVLLLPYKED-IPGTLSVRENEASSSNAGLGQEEVEFDGFGDMFNE 660
Query: 661 PEIPIGPIGRPSTSNEVAESSFN----SESDPEEVDNTDAPVSVESCLTFFTKPELLSNE 720
PEI GPI PS +NEVAE+ F S+SDP+EVD++++PVSVESCL+ F KPELLS++
Sbjct: 661 PEIAEGPIIGPSLANEVAETGFMAGSISDSDPDEVDDSNSPVSVESCLSHFIKPELLSDD 720
Query: 721 NGYNCEKCSKRLQQQRLEMKKQSKVACKAVANGCQTAVGGDISSCNEDSSVEVKNK---R 780
N +NCE C+K L+ Q+L+ KK+ QT +G D+ + +E S++++++
Sbjct: 721 NAWNCENCAKILRHQKLKAKKK------------QTKMGKDLLNGSETRSLDMEHQCPNG 780
Query: 781 NMNLTNGSISYSSGESSNLKKNVDCSSQDCSKPVNCQKSKTDPPVL-DEDEAKVDKDMNP 840
++NG IS SSG+SS +Q+ K N Q S+ D L D K + ++
Sbjct: 781 VRTISNGDIS-SSGDSSVFHN----KNQNGVKVENGQTSELDSVELEDASPLKSNSSVSS 840
Query: 841 GLSRSSGCNNTRNQENSDDKSSCSLPNDEPAKTNIEHLSSHLAVGNQSEKSEDGEMDSDS 900
C TR + SC++ N +T +SH+ QS S+D E+DS++
Sbjct: 841 KCYAQEKCGGTRTID------SCNVENHSGNET-FRQSNSHMTENCQSGVSDDEELDSEN 900
Query: 901 TIVNVKRDATKRFLIHKAPPILTIHMKRFSPDARGRYSKLNGHVRFKETIDLKPYLDTRC 960
+ VKR+ATKR LI+KAPP+LTIH+KRF DARGR SKLNGHV F+ETIDL+PYLD RC
Sbjct: 901 --LKVKRNATKRVLINKAPPVLTIHLKRFCQDARGRLSKLNGHVNFRETIDLRPYLDPRC 960
Query: 961 ADRDKCSYRLVGVVEHSGSMRGGHYVAYVRGGNRKRSSGEAEEDASVWYYASDAVVDEVT 979
D D C+Y LVGVVEHSG+MRGGHY+AYVRGG +++ + + S WYY SD V + +
Sbjct: 961 EDADNCNYSLVGVVEHSGTMRGGHYIAYVRGGEKRKGTTGIDHVGSQWYYVSDQHVRQAS 1004
BLAST of Cucsa.256480 vs. TAIR10
Match:
AT1G04860.1 (AT1G04860.1 ubiquitin-specific protease 2)
HSP 1 Score: 538.5 bits (1386), Expect = 8.6e-153
Identity = 341/859 (39.70%), Postives = 461/859 (53.67%), Query Frame = 1
Query: 1 MGKKVKKKTNRAPPKEKRVSSSSPRKVPQPSNTTVETADEEISIVKEKSQCGHLDKCFNL 60
MGKK KKK RAP KE + S + +P + E A+ ++ VKE C H DK NL
Sbjct: 1 MGKKAKKKA-RAPTKEIQTMEISKKVSEEPPSQAGEIAEGDVKAVKETQACVHFDKALNL 60
Query: 61 NELSSKLGSAEPISCEDCQDSSADRRGGRGKARHGKKKGGTSVDVKTDAKAIWICLQCGH 120
++ K+ S+ I C +C + +RG + K GKK +S D K++ KAIW+CL+CG
Sbjct: 61 EKVLDKIKSSRQIKCAECNEGVYGKRGTKAKGSKGKKDFSSS-DPKSNNKAIWLCLECGC 120
Query: 121 YACGGIGLPTNSQSHAVRHVRQTRHPVVIQFENPQLRWCFSCNTLLPVEKTEENGEQKDS 180
Y CGG+GLP QSH +RH R TRH +VIQ+ENPQLRWCF C LLPVEK E+NGE+KD
Sbjct: 121 YVCGGVGLPNGPQSHVLRHSRVTRHRLVIQWENPQLRWCFPCQLLLPVEK-EDNGEKKDV 180
Query: 181 LSNVVKLIRDRSMES-THVDVENTRYTSSEVTAATKPESSVLSDLDRRNQYIVKGLINLG 240
LS VVKLI+ RS+ + D+E+ S +T+ K E +V SD++ R+ Y+V+GL+NLG
Sbjct: 181 LSEVVKLIKGRSLNNLASSDIEDQCSGSGSITSDIKLEGAVTSDIEARDGYVVRGLVNLG 240
Query: 241 NTCFFNSILQNLLAIDMLRDHFVKLEECV--GPLTIALKKIFIEARTESRMKSSINPRSV 300
NTCFFNSI+QNLL++D LRDHF+K GPL +L+K+F E + E+ +KS INPR+
Sbjct: 241 NTCFFNSIMQNLLSLDRLRDHFLKENGSGVGGPLASSLRKLFTETKPEAGLKSVINPRAF 300
Query: 301 FGCISSKAPQFKGYEQHDSHELLRVLLDALSSEELTSRKMTNSKEERISGNPTPTFVDEM 360
FG SKAPQF+GY+QHDSHELLR LLD+LS+EE RK + + + T ++ +
Sbjct: 301 FGSFCSKAPQFRGYDQHDSHELLRCLLDSLSTEESALRKKRGVSD---NDEKSTTLIESV 360
Query: 361 FGGQISSAVCCKECGHTSTVYEPFLDLSLPVPMKKPLAKKVQPVSRAKKTKVPPKRNGKT 420
FGG+ SS V C ECGH+S VYEPFLDLSLPVP KK KK QPVSRAKK K+PPKR K
Sbjct: 361 FGGETSSIVSCMECGHSSKVYEPFLDLSLPVPFKKSPPKKPQPVSRAKKAKLPPKRVPKN 420
Query: 421 IPKTNKVSDIVPIQIAS--------------------------------VPSSSNESSLP 480
+ K +KVS ++P + S PS +
Sbjct: 421 VSKVSKVSKVLPGMVLSELNSSGKSMAVTADSDTSCSSLAPLDNGPVLETPSVLTLDNNQ 480
Query: 481 SEASASSTTT---------IMEKTSTSQNVSDAKESGKE-------------------IS 540
+ SAS + T I +TS + D +E G + S
Sbjct: 481 ASESASQSDTGFDGSWLDFIGPETSGDETNLDMQEDGIDNVITAEVNQIVPSPNIVANSS 540
Query: 541 VENGGECASDNLT-----WMDFLEPELNADNCDISTTQDSENNIEVFISDNSQQSVSGLS 600
V +G + N + + + E N D D+ Q E IS Q+
Sbjct: 541 VSSGDQTLEGNTERLMQDYEEIAKAEANLDEKDVQAMQSDECPATSGISAEFSQA----- 600
Query: 601 MPVSSLHSEPNQRPDFSSVNSWNDEA-PLQVQASEVLLLPYKEESSTAEVAKEDDQASSS 660
S + +P SSVN W++E PL V S++L +PYKE S + + + +ASSS
Sbjct: 601 ---SCIGCDPGIGESSSSVNPWDEEELPLVVADSQILYMPYKEISCNDKSVEGECEASSS 660
Query: 661 ILGCAQE--DFDGFGLGDMFDEPEIPIGPIGRPSTSNE---VAESSFNSESDPEEVDNTD 720
+ E + D G +FDEPE GP+ P + E V +F+SESDPEE+D++D
Sbjct: 661 FVTGDHEPQNSDFVDFGGLFDEPETTEGPVFGPPSKAEASGVGFMAFSSESDPEEIDDSD 720
Query: 721 APVSVESCLTFFTKPELLSNENGYNCEKCSKRLQQQRLEMKKQSKVACKAVANGCQTAVG 780
PVSVE CL FTK E+LS++N +NCE CSK L+ QRL K++S
Sbjct: 721 LPVSVERCLGHFTKHEILSDDNAWNCENCSKNLKLQRLREKRKS---------------- 780
HSP 2 Score: 190.7 bits (483), Expect = 4.4e-48
Identity = 110/239 (46.03%), Postives = 152/239 (63.60%), Query Frame = 1
Query: 752 QDCSKPVNCQKSKTDPPVLDEDEAKVDKDMNPGLSRSSGCNNTRNQENSDD---KSSCSL 811
++CSK + Q+ + + +EDE SRSS +N +EN D+ ++
Sbjct: 733 ENCSKNLKLQRLR-EKRKSNEDE-----------SRSSNTSNGWVKENEDEGFGETEILA 792
Query: 812 PNDEPAKTNI--EHLSS-------HLAVGNQSEKSEDGEMDSDSTIVNVKRDATKRFLIH 871
+P T+ +H S H A ++S+ ++D + DS+ ++ VKRDATK+ LI+
Sbjct: 793 VKQDPNDTSCVKDHSSDGRKAARIHSADESESKGTQDEDEDSEK-VITVKRDATKKVLIN 852
Query: 872 KAPPILTIHMKRFSPDARGRYSKLNGHVRFKETIDLKPYLDTRCADRDKCSYRLVGVVEH 931
KAPP+LTIH+KRFS D RGR SKLNGHV FKE IDL+ Y+D+RC+ D YRL G+VEH
Sbjct: 853 KAPPVLTIHLKRFSQDLRGRLSKLNGHVAFKEVIDLRQYMDSRCSGEDPPVYRLAGLVEH 912
Query: 932 SGSMRGGHYVAYVRGGNRKRSSGEAEEDASVWYYASDAVVDEVTLDRVLGCEAYILFYE 979
SG+MRGGHYVAYVRGG R + E + ++ WY SDA V +V+L++VL EAYILFYE
Sbjct: 913 SGTMRGGHYVAYVRGGQRVK---ETDSSSTAWYNVSDAYVRQVSLEKVLHSEAYILFYE 955
BLAST of Cucsa.256480 vs. TAIR10
Match:
AT2G32780.1 (AT2G32780.1 ubiquitin-specific protease 1)
HSP 1 Score: 378.6 bits (971), Expect = 1.1e-104
Identity = 238/617 (38.57%), Postives = 343/617 (55.59%), Query Frame = 1
Query: 31 SNTTVETADEEISIVKEKSQCGHLDKCFNLNELSSKLGSAEPISCEDCQDSSADRRGGRG 90
S +VE +E + EK C H DK +L++L + S + I C +C + +RG R
Sbjct: 12 SEQSVEVTEEFAVTMTEKRSCVHFDKYVDLDKLLKIIKSYQQIKCGECNERVHVKRGSRW 71
Query: 91 KARHGKKKGGTSVDVKTDAKAIWICLQCGHYACGGIGLPTNSQSHAVRHVRQTRHPVVIQ 150
A + ++ +S D AIW+CL+CG+Y CG +GLPT +QSH + H R TRH +VIQ
Sbjct: 72 GASN-RRDWYSSSDQNCARNAIWLCLECGYYVCGDVGLPTEAQSHVMGHNRLTRHRLVIQ 131
Query: 151 FENPQLRWCFSCNTLLPVEKTEENGEQKDSLSNVVKLIRDRSMESTHVDVENTRYTSSEV 210
+NPQLRWCFSC +LLP + EENGE+KD L VVKLIR+RS + E T ++ S
Sbjct: 132 CKNPQLRWCFSCQSLLPFD-NEENGEKKDLLLEVVKLIRERSPNTYSASFE-TEFSCS-- 191
Query: 211 TAATKPESSVLSDLDRRNQYIVKGLINLGNTCFFNSILQNLLAIDMLRDHFVKLEECV-G 270
S+ ++ R+ Y V+GL+NLGNTCFFNS++QNLL++D LR+HF+K + V G
Sbjct: 192 -------GSIYGGIEARDGYAVRGLVNLGNTCFFNSVMQNLLSLDQLREHFLKEDLSVSG 251
Query: 271 PLTIALKKIFIEARTE-SRMKSSINPRSVFGCISSKAPQFKGYEQHDSHELLRVLLDALS 330
PL +LK++F E+ +E S ++ INPR +F + S+APQF+GY+QHDSHELLR LLD LS
Sbjct: 252 PLVSSLKELFAESNSEASVFRNEINPRDLFFSVCSQAPQFRGYQQHDSHELLRCLLDGLS 311
Query: 331 SEELTSRKMTNSKEERISGN-PTPTFVDEMFGGQISSAVCCKECGHTSTVYEPFLDLSLP 390
EE + RK + S PT +D +FGG+ISS V C ECGH S VYEPF+DLSLP
Sbjct: 312 IEESSLRKKLGVSDSNDSSTYQKPTLIDSVFGGEISSTVSCLECGHFSKVYEPFMDLSLP 371
Query: 391 VPMKKPLAKKVQPVSRAKKTKVPPKRNGKTIPKTNKVSDIVPIQIASVPSSSNESSLPSE 450
VP K KK Q +S+AK+ +NG + K ++V +++ P+S + S++P
Sbjct: 372 VPSMKLPPKKQQILSQAKEV----LKNG-AVSKDSEV-------VSAKPASDHNSTVP-- 431
Query: 451 ASASSTTTIMEKTSTSQNVSDAKESGKEISVENGGECASDNLTWMDFLEPELNADNCDIS 510
S I + TS N +D E N + E ++ ++
Sbjct: 432 -LFPSDHKIQSRPETSDNETDLVLLSDVSDTAPSTEAKGVNQILVGSTETLMHDNDRTGK 491
Query: 511 TTQDSENNIEVFISDNSQQSVSGLSMPV--SSLHSEPNQRPDFSSVNSWND-EAPLQVQA 570
T D E+ N + S SG+S + + + P+ SS N D E L V
Sbjct: 492 TVPDKEDVRAT--QSNEETSASGISAVIDEAQVCGCPDLEQSSSSANQGADEELALMVAD 551
Query: 571 SEVLLLPYKEESSTAEVAKEDDQASSSILGCAQEDFDGFGLGDMFDEPEIPIGPIGRPST 630
S+VL +PYK+ + + +SS + G + D F DEPEI GP+ RP +
Sbjct: 552 SQVLYMPYKDHLFYDDYMVA-EASSSFVSGDHEPKKDYFDFSSFLDEPEIREGPVFRPLS 598
Query: 631 SNEVAESSFNSESDPEE 642
+EV E+ F ++ ++
Sbjct: 612 KSEVYEAGFKADCSDDK 598
HSP 2 Score: 364.0 bits (933), Expect = 2.9e-100
Identity = 265/815 (32.52%), Postives = 409/815 (50.18%), Query Frame = 1
Query: 31 SNTTVETADEEISIVKEKSQCGHLDKCFNLNELSSKLGSAEPISCEDCQDSSADRRGGRG 90
S +VE +E + EK C H DK +L++L + S + I C +C + +RG R
Sbjct: 12 SEQSVEVTEEFAVTMTEKRSCVHFDKYVDLDKLLKIIKSYQQIKCGECNERVHVKRGSRW 71
Query: 91 KARHGKKKGGTSVDVKTDAKAIWICLQCGHYACGGIGLPTNSQSHAVRHVRQTRHPVVIQ 150
A + ++ +S D AIW+CL+CG+Y CG +GLPT +QSH + H R TRH +VIQ
Sbjct: 72 GASN-RRDWYSSSDQNCARNAIWLCLECGYYVCGDVGLPTEAQSHVMGHNRLTRHRLVIQ 131
Query: 151 FENPQLRWCFSCNTLLPVEKTEENGEQKDSLSNVVKLIRDRSMESTHVDVENTRYTSSEV 210
+NPQLRWCFSC +LLP + EENGE+KD L VVKLIR+RS + E T ++ S
Sbjct: 132 CKNPQLRWCFSCQSLLPFDN-EENGEKKDLLLEVVKLIRERSPNTYSASFE-TEFSCS-- 191
Query: 211 TAATKPESSVLSDLDRRNQYIVKGLINLGNTCFFNSILQNLLAIDMLRDHFVKLEECV-G 270
S+ ++ R+ Y V+GL+NLGNTCFFNS++QNLL++D LR+HF+K + V G
Sbjct: 192 -------GSIYGGIEARDGYAVRGLVNLGNTCFFNSVMQNLLSLDQLREHFLKEDLSVSG 251
Query: 271 PLTIALKKIFIEARTESRM-KSSINPRSVFGCISSKAPQFKGYEQHDSHELLRVLLDALS 330
PL +LK++F E+ +E+ + ++ INPR +F + S+APQF+GY+QHDSHELLR LLD LS
Sbjct: 252 PLVSSLKELFAESNSEASVFRNEINPRDLFFSVCSQAPQFRGYQQHDSHELLRCLLDGLS 311
Query: 331 SEELTSRKMTNSKEERISGN-PTPTFVDEMFGGQISSAVCCKECGHTSTVYEPFLDLSLP 390
EE + RK + S PT +D +FGG+ISS V C ECGH S VYEPF+DLSLP
Sbjct: 312 IEESSLRKKLGVSDSNDSSTYQKPTLIDSVFGGEISSTVSCLECGHFSKVYEPFMDLSLP 371
Query: 391 VPMKKPLAKKVQPVSRAKK--------------TKVPPKRNGKTIP-------------K 450
VP K KK Q +S+AK+ + P + T+P
Sbjct: 372 VPSMKLPPKKQQILSQAKEVLKNGAVSKDSEVVSAKPASDHNSTVPLFPSDHKIQSRPET 431
Query: 451 TNKVSDIVPIQIASVPSSSNESSLPSEASASSTTTIM-EKTSTSQNVSD-----AKESGK 510
++ +D+V + S + S E+ ++ ST T+M + T + V D A +S +
Sbjct: 432 SDNETDLVLLSDVSDTAPSTEAKGVNQILVGSTETLMHDNDRTGKTVPDKEDVRATQSNE 491
Query: 511 EISVENGGECASDNLTWMDFLEPELNADNCDISTTQDSENNIEVFISDNS---------- 570
E S +G D + E ++ S Q ++ + + ++D+
Sbjct: 492 ETSA-SGISAVIDEAQVCGCPDLEQSSS----SANQGADEELALMVADSQVLYMPYKDHL 551
Query: 571 ---QQSVSGLSMPVSSLHSEPNQRPDFSSVNSWNDEAPLQ-------VQASEVLLLPYKE 630
V+ S S EP + D+ +S+ DE ++ + SEV +K
Sbjct: 552 FYDDYMVAEASSSFVSGDHEPKK--DYFDFSSFLDEPEIREGPVFRPLSKSEVYEAGFKA 611
Query: 631 ESSTAEV--AKEDDQASSSILGCAQEDFDGFGLGDMFDEPEIPIGPIGRPSTSNEVAESS 690
+ S + A + + +SS I +++ D FDEPEI P RP + +EV+E+
Sbjct: 612 DCSDDKTVSAGKGEASSSFISSDHEQNIDYVDFSSFFDEPEISERPFFRPLSKSEVSEAG 671
Query: 691 FNSESDPEEVDNTDAPVSVESCLTFFTKP-----ELLSNENGYNCEKCSKRLQQQRLEMK 750
F ++ D T + E+ +F + + + + ++ + S+R + L
Sbjct: 672 FKADCSD---DKTVSAGKGEASSSFVSSDHEQNIDYVDFSSFFDEPEISERPFFRPLSKS 731
Query: 751 KQSKVACKAVANGCQTAVGGDISSCNEDSSVEVKNKRNMNLT--NGSISYSSGESSNLKK 781
+ S+ AV+ +T G + + S + N+RN++ I G S
Sbjct: 732 EVSEAGFMAVSGNDKTVRAGKGETFS--SFMSGDNERNIDYVEFTNRIFDDRGTSERPVF 791
HSP 3 Score: 186.4 bits (472), Expect = 8.3e-47
Identity = 160/546 (29.30%), Postives = 255/546 (46.70%), Query Frame = 1
Query: 461 STSQNVSDAKESGKEISVENGGECASDNLTWMDFL-EPELNADNC--DISTTQDSENNIE 520
S + VS K + + E D + + F EPE++ +S ++ SE +
Sbjct: 595 SDDKTVSAGKGEASSSFISSDHEQNIDYVDFSSFFDEPEISERPFFRPLSKSEVSEAGFK 654
Query: 521 VFISDNSQQSVSGLSMPVSSLHSEPNQRPDFSSVNSWNDEAPLQVQASEVLLLPYKEESS 580
SD+ S S + S+ Q D+ +S+ DE E+ P+ S
Sbjct: 655 ADCSDDKTVSAGKGEASSSFVSSDHEQNIDYVDFSSFFDEP-------EISERPFFRPLS 714
Query: 581 TAEVAKEDDQASS----SILGCAQEDFDGFGLGD-------------MFDEPEIPIGPIG 640
+EV++ A S ++ E F F GD +FD+ P+
Sbjct: 715 KSEVSEAGFMAVSGNDKTVRAGKGETFSSFMSGDNERNIDYVEFTNRIFDDRGTSERPVF 774
Query: 641 RPSTSNEVAESSF---NSESDPEEVDNTDAPVSVESCLTFFTKPELLSNENGYNCEKCSK 700
P + +V+E+ F +S+SDP +D +++ + ++C
Sbjct: 775 GPPSKAKVSEAGFVAVSSDSDPAVLD---------------------ESDSPVSVDRCLA 834
Query: 701 RLQQQRLEMKKQSKVACKAVANGCQTAVGGDISSCNEDSSVEVKNKRNMNLTNGSISYSS 760
+ + + + + + C+ + + + K + L+N ++ +
Sbjct: 835 QFTKHEI-LSEDNAWHCENCSKNLKL------------QRLREKRRTKEGLSNRWVN-EN 894
Query: 761 GESSNLKKNVDCS-SQDCSKPVNCQKSK---TDPPVLDEDEAKVDKDMNPGLSRSSGCNN 820
G SS + D S +Q C N K+ T P E+E+ +D +
Sbjct: 895 GASSAFDECRDSSLNQSCIDLENGYKAAPPITKLPNCKEEESAID-------------DG 954
Query: 821 TRNQENSDDKSSCSLPNDEPAKTNIEHLSSHLAVGNQSEKSEDGEMDSDSTIVNVKRDAT 880
+EN+ S+ E E +SS A N+ E ED +DS+ IV KRDA
Sbjct: 955 FVGEENTKQAPITSVT--ETPLLGGETISSQPASDNECENWEDLAVDSEEVIV--KRDAR 1014
Query: 881 KRFLIHKAPPILTIHMKRFSPDARGRYSKLNGHVRFKETIDLKPYLDTRCADRDKCSYRL 940
K+ LI+KAPP+LTIH+KRFS DARGR SKL+GHV F+E IDL Y+DTRC++ D+ YRL
Sbjct: 1015 KKVLINKAPPVLTIHLKRFSQDARGRVSKLSGHVDFQEFIDLSKYMDTRCSEEDEPVYRL 1074
Query: 941 VGVVEHSGSMRGGHYVAYVRGGNRKRSSGEAEE-DASVWYYASDAVVDEVTLDRVLGCEA 979
G+VEH G+M GHYV+Y+RGG+++R + +E ++S+WY+ASD+ V +L+ VL EA
Sbjct: 1075 AGLVEHLGAMSRGHYVSYIRGGHKERRDSDTKEPNSSIWYHASDSQVRPASLEEVLRSEA 1081
BLAST of Cucsa.256480 vs. TAIR10
Match:
AT1G32850.1 (AT1G32850.1 ubiquitin-specific protease 11)
HSP 1 Score: 88.2 bits (217), Expect = 3.1e-17
Identity = 113/489 (23.11%), Postives = 193/489 (39.47%), Query Frame = 1
Query: 234 GLINLGNTCFFNSILQNLLAIDMLRDHFVKLEECV----------GPLTIALKKIFIEAR 293
GL NLGNTCF NS LQ L + ++F++ G L IA ++ +
Sbjct: 302 GLQNLGNTCFMNSTLQCLAHTPPIVEYFLQDYRSDINAKNPLGMRGELAIAFGELL--RK 361
Query: 294 TESRMKSSINPRSVFGCISSKAPQFKGYEQHDSHELLRVLLDALSSEELTSRKMTNSKEE 353
S ++++ PR+ ++ APQF GY QHDS E+L LLD L + ++ + +
Sbjct: 362 LWSSGQNTVAPRAFKTKLARFAPQFSGYNQHDSQEMLAFLLDGLHEDLNKVKRKPYIEAK 421
Query: 354 RISGNPTPTFVDE---------------MFGGQISSAVCCKECGHTSTVYEPFLDLSLPV 413
G P +E +F GQ S + C +CG S ++PF+ LSLP+
Sbjct: 422 DSDGRPDDEVAEEKWKYHKARNDSVIVDVFQGQYKSTLVCPDCGKISITFDPFMYLSLPL 481
Query: 414 PMKKPLAKKVQPVSRAKKTKVPPKRNGKTIPKTNKVSDIVPIQIASVPSSSNESSLPSEA 473
P + + V V + +P T+PK D+ + ++ES L +E
Sbjct: 482 PSSRTRSMTV-TVFYGDGSHLPMPYT-VTVPKDGSCRDLSNALGTACCLDNDESLLLAEV 541
Query: 474 SASSTTTIMEKTSTSQNVSDAKESGKEISVENGGECASDNLTWMDFL--EPELNADNCD- 533
E + + ++ K++ ++ ++ L E E ++D
Sbjct: 542 YDHKVFKYYE--NPRELLNGIKDNEHIVAYRFKQMHKGPGKVKLEILHGEQEKSSDRGPK 601
Query: 534 -ISTTQDSENNIEVFISDNSQQSVSGLSMPVSSLHSEPNQRPDFSSVNSWNDEAPLQVQA 593
T + N E + S+SGL P+ +H VNS N+ + ++
Sbjct: 602 CFGTPLVTYINKEPLSGTDIATSISGLLSPLRRVHMS-------CVVNSGNENGHVPDES 661
Query: 594 SEVLLLPYKEESSTAEVAKEDDQASSSILGCAQEDFDGFGLGDMFDEPEIPIGPIGRPST 653
S +L S E D + S S+L D+ F L + + + G + +
Sbjct: 662 SRSIL------SRDTETEDNDRELSLSLL----RDYYSFNLQPLESDSVVNPGSVTKVLV 721
Query: 654 S-NEVAESSFNSE--SDPEEVDNT-------DAPVSVESCLTFFTKPELLSNENGYNCEK 684
NE ++S +D +V +S+ SCL F E L ++ + C
Sbjct: 722 KWNEKEHEKYDSSYLNDLPKVHKNVLAKKTMQEGISLFSCLEAFLAEEPLGPDDMWYCPG 767
BLAST of Cucsa.256480 vs. TAIR10
Match:
AT4G10590.2 (AT4G10590.2 ubiquitin-specific protease 10)
HSP 1 Score: 84.7 bits (208), Expect = 3.4e-16
Identity = 127/521 (24.38%), Postives = 198/521 (38.00%), Query Frame = 1
Query: 218 SSVLSDLDRRNQYIVKGLINLGNTCFFNSILQNLLAIDMLRDHFVK-----------LEE 277
S+ LS L + + + GL NLGNTCF NS LQ L + ++F++ L
Sbjct: 288 SNSLSILGKGEKGGLAGLSNLGNTCFMNSALQCLAHTPPIVEYFLQDYSDDINRDNPLGM 347
Query: 278 CVGPLTIA----LKKIFIEARTESRMKSSINPRSVFGCISSKAPQFKGYEQHDSHELLRV 337
C G L IA LKK++ R +S+ PR+ ++ APQF GY QHDS ELL
Sbjct: 348 C-GELAIAFGDLLKKLWSSGR------NSVAPRAFKTKLARFAPQFSGYNQHDSQELLAF 407
Query: 338 LLDALSSEELTSRKMTNSKEERISGNPTPTFVDEMFG---------------GQISSAVC 397
LLD L + ++ + + P +E++ GQ S +
Sbjct: 408 LLDGLHEDLNKVKRKPYIELKDSDSRPDDEVAEELWNYHKARNDSVIVDVCQGQYKSTLV 467
Query: 398 CKECGHTSTVYEPFLDLSLPVPMKKPLAKKVQPVSRAKKTKVPPKRNGKTIPKTNKVSDI 457
C CG S ++PF+ LS+P+P L + + P +PK + D+
Sbjct: 468 CPACGKISITFDPFMYLSVPLP--STLTRSMTVTVFYCDGSHLPMPYTVIVPKNGSIRDL 527
Query: 458 VPIQIASVPSSSNESSLPSEASASSTTTIMEKTSTS-QNVSD------------AKESGK 517
+ + + +ES L +E E S ++ D K SGK
Sbjct: 528 ITALGTACLLAEDESLLLAEVYDHKIFKYFENPLDSLSSIKDDEHIVAYRLNQMPKGSGK 587
Query: 518 -EISVENGGECASDNLTWMDFLEPELNADNCDISTTQDSENNIEVFISDNSQQSVSGLSM 577
++ + +GG+ LE D T + N E + +S
Sbjct: 588 AKLEILHGGQ-------KRPILESVRGRDVKLFGTPFVTYVNTEPLSGADIDAVLSRFLS 647
Query: 578 PVSSLHSEPNQRPDFSSVNSWNDEAPLQVQASEVLLLPYKEESSTAEVAKEDDQASSSIL 637
P+ +H+ + S N +A + +ASE+L P E + D + S I
Sbjct: 648 PLHKVHAP--SKIHNGSENGHLPDATVD-EASEILSSPDTEIDDAS-----DRELSFRIF 707
Query: 638 GCAQEDFDGFGLGDMFDEPEIPIGPIGRPSTS-NEVAESSFNSE--SDPEEVDNT----- 684
D G + E I +G R NE ++S SD EV T
Sbjct: 708 ---LTDERGLNFKPLQSESSISLGIATRVLVEWNEGEHERYDSSYLSDLPEVHKTSFSAK 767
BLAST of Cucsa.256480 vs. TAIR10
Match:
AT4G10570.1 (AT4G10570.1 ubiquitin-specific protease 9)
HSP 1 Score: 82.0 bits (201), Expect = 2.2e-15
Identity = 121/522 (23.18%), Postives = 205/522 (39.27%), Query Frame = 1
Query: 221 LSDLDRRNQYIVKGLINLGNTCFFNSILQNLLAIDMLRDHFVK-----------LEECVG 280
LS L + + + GL NLGNTCF NS LQ L + ++F++ L C G
Sbjct: 292 LSILGKGEKGGLAGLSNLGNTCFMNSALQCLAHTPPIVEYFLQDYSDDINRDNPLGMC-G 351
Query: 281 PLTIA----LKKIFIEARTESRMKSSINPRSVFGCISSKAPQFKGYEQHDSHELLRVLLD 340
L IA LKK++ R +++ PR+ ++ APQF GY QHDS ELL LLD
Sbjct: 352 ELAIAFGDLLKKLWSSGR------NAVAPRAFKTKLARFAPQFSGYNQHDSQELLAFLLD 411
Query: 341 ALSSEELTSRKMTNSKEERISGNPTPTFVDEMFG---------------GQISSAVCCKE 400
L + ++ + + P +E++ GQ S + C
Sbjct: 412 GLHEDLNKVKRKPYIELKDSDSRPDDEVAEELWNYHKARNDSVIVDVCQGQYKSTLVCPV 471
Query: 401 CGHTSTVYEPFLDLSLPVPMKKPLAKKVQPVSRAKKTKVPPKRNGKTIPKTNKVSDIVPI 460
CG S ++PF+ LS+P+P + + V +++P +PK + D++
Sbjct: 472 CGKISITFDPFMYLSVPLPSTLTRSMTI-TVFYCDGSRLPMPYT-VIVPKQGSIRDLITA 531
Query: 461 QIASVPSSSNESSLPSEA-----------SASSTTTIM-EKTSTSQNVSDAKESGKEISV 520
+ + +ES L +E S + I ++ + ++ + ++ +
Sbjct: 532 LGTACCLAEDESLLLAEVYDHKIFRYFEIPLDSLSAIKDDEHIVAYRLNQIPKGSRKAKL 591
Query: 521 E--NGGECASDNLTWMDFLEPELNADNCDISTTQDSENNIEVFISDNSQQSVSGLSMPVS 580
E +GG+ + L+ +D T + N E + +SG P+
Sbjct: 592 EILHGGQERA-------VLDSVRGSDVKLFGTPFVTYVNTEPLSGTDIDAVISGFLSPLH 651
Query: 581 SLHSEPNQRPDFSSVNSWNDEAPLQ----VQASEVLLLPYKEESSTAEVAKEDDQASSSI 640
+H+ S +++ +D L QAS +L P E + + D + S I
Sbjct: 652 KVHAP-------SKIHNGSDNGHLADATVDQASGILSSPDTEIDNAS-----DRELSFRI 711
Query: 641 LGCAQEDFDGFGLGDMFDEPEIPIGPIGRPSTS-NEVAESSFNSE--SDPEEVDNT---- 684
D G + + E I G + R NE ++S SD EV T
Sbjct: 712 F---LTDERGLNIKPLQSESSISPGTVTRVLVEWNEGEHERYDSSYLSDLPEVHKTSFSA 771
BLAST of Cucsa.256480 vs. NCBI nr
Match:
gi|449433181|ref|XP_004134376.1| (PREDICTED: ubiquitin carboxyl-terminal hydrolase 2 [Cucumis sativus])
HSP 1 Score: 1967.2 bits (5095), Expect = 0.0e+00
Identity = 980/980 (100.00%), Postives = 980/980 (100.00%), Query Frame = 1
Query: 1 MGKKVKKKTNRAPPKEKRVSSSSPRKVPQPSNTTVETADEEISIVKEKSQCGHLDKCFNL 60
MGKKVKKKTNRAPPKEKRVSSSSPRKVPQPSNTTVETADEEISIVKEKSQCGHLDKCFNL
Sbjct: 1 MGKKVKKKTNRAPPKEKRVSSSSPRKVPQPSNTTVETADEEISIVKEKSQCGHLDKCFNL 60
Query: 61 NELSSKLGSAEPISCEDCQDSSADRRGGRGKARHGKKKGGTSVDVKTDAKAIWICLQCGH 120
NELSSKLGSAEPISCEDCQDSSADRRGGRGKARHGKKKGGTSVDVKTDAKAIWICLQCGH
Sbjct: 61 NELSSKLGSAEPISCEDCQDSSADRRGGRGKARHGKKKGGTSVDVKTDAKAIWICLQCGH 120
Query: 121 YACGGIGLPTNSQSHAVRHVRQTRHPVVIQFENPQLRWCFSCNTLLPVEKTEENGEQKDS 180
YACGGIGLPTNSQSHAVRHVRQTRHPVVIQFENPQLRWCFSCNTLLPVEKTEENGEQKDS
Sbjct: 121 YACGGIGLPTNSQSHAVRHVRQTRHPVVIQFENPQLRWCFSCNTLLPVEKTEENGEQKDS 180
Query: 181 LSNVVKLIRDRSMESTHVDVENTRYTSSEVTAATKPESSVLSDLDRRNQYIVKGLINLGN 240
LSNVVKLIRDRSMESTHVDVENTRYTSSEVTAATKPESSVLSDLDRRNQYIVKGLINLGN
Sbjct: 181 LSNVVKLIRDRSMESTHVDVENTRYTSSEVTAATKPESSVLSDLDRRNQYIVKGLINLGN 240
Query: 241 TCFFNSILQNLLAIDMLRDHFVKLEECVGPLTIALKKIFIEARTESRMKSSINPRSVFGC 300
TCFFNSILQNLLAIDMLRDHFVKLEECVGPLTIALKKIFIEARTESRMKSSINPRSVFGC
Sbjct: 241 TCFFNSILQNLLAIDMLRDHFVKLEECVGPLTIALKKIFIEARTESRMKSSINPRSVFGC 300
Query: 301 ISSKAPQFKGYEQHDSHELLRVLLDALSSEELTSRKMTNSKEERISGNPTPTFVDEMFGG 360
ISSKAPQFKGYEQHDSHELLRVLLDALSSEELTSRKMTNSKEERISGNPTPTFVDEMFGG
Sbjct: 301 ISSKAPQFKGYEQHDSHELLRVLLDALSSEELTSRKMTNSKEERISGNPTPTFVDEMFGG 360
Query: 361 QISSAVCCKECGHTSTVYEPFLDLSLPVPMKKPLAKKVQPVSRAKKTKVPPKRNGKTIPK 420
QISSAVCCKECGHTSTVYEPFLDLSLPVPMKKPLAKKVQPVSRAKKTKVPPKRNGKTIPK
Sbjct: 361 QISSAVCCKECGHTSTVYEPFLDLSLPVPMKKPLAKKVQPVSRAKKTKVPPKRNGKTIPK 420
Query: 421 TNKVSDIVPIQIASVPSSSNESSLPSEASASSTTTIMEKTSTSQNVSDAKESGKEISVEN 480
TNKVSDIVPIQIASVPSSSNESSLPSEASASSTTTIMEKTSTSQNVSDAKESGKEISVEN
Sbjct: 421 TNKVSDIVPIQIASVPSSSNESSLPSEASASSTTTIMEKTSTSQNVSDAKESGKEISVEN 480
Query: 481 GGECASDNLTWMDFLEPELNADNCDISTTQDSENNIEVFISDNSQQSVSGLSMPVSSLHS 540
GGECASDNLTWMDFLEPELNADNCDISTTQDSENNIEVFISDNSQQSVSGLSMPVSSLHS
Sbjct: 481 GGECASDNLTWMDFLEPELNADNCDISTTQDSENNIEVFISDNSQQSVSGLSMPVSSLHS 540
Query: 541 EPNQRPDFSSVNSWNDEAPLQVQASEVLLLPYKEESSTAEVAKEDDQASSSILGCAQEDF 600
EPNQRPDFSSVNSWNDEAPLQVQASEVLLLPYKEESSTAEVAKEDDQASSSILGCAQEDF
Sbjct: 541 EPNQRPDFSSVNSWNDEAPLQVQASEVLLLPYKEESSTAEVAKEDDQASSSILGCAQEDF 600
Query: 601 DGFGLGDMFDEPEIPIGPIGRPSTSNEVAESSFNSESDPEEVDNTDAPVSVESCLTFFTK 660
DGFGLGDMFDEPEIPIGPIGRPSTSNEVAESSFNSESDPEEVDNTDAPVSVESCLTFFTK
Sbjct: 601 DGFGLGDMFDEPEIPIGPIGRPSTSNEVAESSFNSESDPEEVDNTDAPVSVESCLTFFTK 660
Query: 661 PELLSNENGYNCEKCSKRLQQQRLEMKKQSKVACKAVANGCQTAVGGDISSCNEDSSVEV 720
PELLSNENGYNCEKCSKRLQQQRLEMKKQSKVACKAVANGCQTAVGGDISSCNEDSSVEV
Sbjct: 661 PELLSNENGYNCEKCSKRLQQQRLEMKKQSKVACKAVANGCQTAVGGDISSCNEDSSVEV 720
Query: 721 KNKRNMNLTNGSISYSSGESSNLKKNVDCSSQDCSKPVNCQKSKTDPPVLDEDEAKVDKD 780
KNKRNMNLTNGSISYSSGESSNLKKNVDCSSQDCSKPVNCQKSKTDPPVLDEDEAKVDKD
Sbjct: 721 KNKRNMNLTNGSISYSSGESSNLKKNVDCSSQDCSKPVNCQKSKTDPPVLDEDEAKVDKD 780
Query: 781 MNPGLSRSSGCNNTRNQENSDDKSSCSLPNDEPAKTNIEHLSSHLAVGNQSEKSEDGEMD 840
MNPGLSRSSGCNNTRNQENSDDKSSCSLPNDEPAKTNIEHLSSHLAVGNQSEKSEDGEMD
Sbjct: 781 MNPGLSRSSGCNNTRNQENSDDKSSCSLPNDEPAKTNIEHLSSHLAVGNQSEKSEDGEMD 840
Query: 841 SDSTIVNVKRDATKRFLIHKAPPILTIHMKRFSPDARGRYSKLNGHVRFKETIDLKPYLD 900
SDSTIVNVKRDATKRFLIHKAPPILTIHMKRFSPDARGRYSKLNGHVRFKETIDLKPYLD
Sbjct: 841 SDSTIVNVKRDATKRFLIHKAPPILTIHMKRFSPDARGRYSKLNGHVRFKETIDLKPYLD 900
Query: 901 TRCADRDKCSYRLVGVVEHSGSMRGGHYVAYVRGGNRKRSSGEAEEDASVWYYASDAVVD 960
TRCADRDKCSYRLVGVVEHSGSMRGGHYVAYVRGGNRKRSSGEAEEDASVWYYASDAVVD
Sbjct: 901 TRCADRDKCSYRLVGVVEHSGSMRGGHYVAYVRGGNRKRSSGEAEEDASVWYYASDAVVD 960
Query: 961 EVTLDRVLGCEAYILFYEIT 981
EVTLDRVLGCEAYILFYEIT
Sbjct: 961 EVTLDRVLGCEAYILFYEIT 980
BLAST of Cucsa.256480 vs. NCBI nr
Match:
gi|659075844|ref|XP_008438362.1| (PREDICTED: ubiquitin carboxyl-terminal hydrolase 2 [Cucumis melo])
HSP 1 Score: 1866.7 bits (4834), Expect = 0.0e+00
Identity = 928/980 (94.69%), Postives = 955/980 (97.45%), Query Frame = 1
Query: 1 MGKKVKKKTNRAPPKEKRVSSSSPRKVPQPSNTTVETADEEISIVKEKSQCGHLDKCFNL 60
MGKKVKKKTNRAPPKEKRVSS+SPRKVPQPSNTTVETADEE+SIVKEKSQC HLDKCFNL
Sbjct: 1 MGKKVKKKTNRAPPKEKRVSSNSPRKVPQPSNTTVETADEEVSIVKEKSQCRHLDKCFNL 60
Query: 61 NELSSKLGSAEPISCEDCQDSSADRRGGRGKARHGKKKGGTSVDVKTDAKAIWICLQCGH 120
NELSSKLGSAEPISCEDCQDSSADRRG RGKARHGKKKGGTSVDVKTDAKAIWICLQCGH
Sbjct: 61 NELSSKLGSAEPISCEDCQDSSADRRGSRGKARHGKKKGGTSVDVKTDAKAIWICLQCGH 120
Query: 121 YACGGIGLPTNSQSHAVRHVRQTRHPVVIQFENPQLRWCFSCNTLLPVEKTEENGEQKDS 180
YACGGIGLPTNSQSHAVRHVRQTRHPVVIQFENPQLRWCFSCNTLLPVEKTEENGEQKD
Sbjct: 121 YACGGIGLPTNSQSHAVRHVRQTRHPVVIQFENPQLRWCFSCNTLLPVEKTEENGEQKDG 180
Query: 181 LSNVVKLIRDRSMESTHVDVENTRYTSSEVTAATKPESSVLSDLDRRNQYIVKGLINLGN 240
LSNVVKLIRDRSMEST VDVENTRY S+EVTAATK ESSVL DLDRRNQY+VKGLINLGN
Sbjct: 181 LSNVVKLIRDRSMESTPVDVENTRYASNEVTAATKSESSVLIDLDRRNQYVVKGLINLGN 240
Query: 241 TCFFNSILQNLLAIDMLRDHFVKLEECVGPLTIALKKIFIEARTESRMKSSINPRSVFGC 300
TCFFNSILQNLLAIDMLRDHFVKLEEC+GPLTIALKKIFIEARTESRMK SINPRSVFGC
Sbjct: 241 TCFFNSILQNLLAIDMLRDHFVKLEECIGPLTIALKKIFIEARTESRMKGSINPRSVFGC 300
Query: 301 ISSKAPQFKGYEQHDSHELLRVLLDALSSEELTSRKMTNSKEERISGNPTPTFVDEMFGG 360
ISSKAPQFKGYEQHDSHELLRVLLDALSSEELTSRKMTNSKEERISGN TPTFVDEMFGG
Sbjct: 301 ISSKAPQFKGYEQHDSHELLRVLLDALSSEELTSRKMTNSKEERISGNLTPTFVDEMFGG 360
Query: 361 QISSAVCCKECGHTSTVYEPFLDLSLPVPMKKPLAKKVQPVSRAKKTKVPPKRNGKTIPK 420
QISSAVCCKECGHTSTVYEPFLDLSLPVPMKKPLAKKVQPVSRAKKTKVPPKRNGKTIPK
Sbjct: 361 QISSAVCCKECGHTSTVYEPFLDLSLPVPMKKPLAKKVQPVSRAKKTKVPPKRNGKTIPK 420
Query: 421 TNKVSDIVPIQIASVPSSSNESSLPSEASASSTTTIMEKTSTSQNVSDAKESGKEISVEN 480
TNKVSDIVP+QIASVPSSSNESSLPSEA ASSTTTIMEKTSTSQNVSDAK+S KEISVEN
Sbjct: 421 TNKVSDIVPVQIASVPSSSNESSLPSEACASSTTTIMEKTSTSQNVSDAKKSEKEISVEN 480
Query: 481 GGECASDNLTWMDFLEPELNADNCDISTTQDSENNIEVFISDNSQQSVSGLSMPVSSLHS 540
GGECASDNLTW+DFLEPELNADNCDIST +DSENN+EVFISD+SQQSVSGLSMPVSS+H+
Sbjct: 481 GGECASDNLTWLDFLEPELNADNCDISTARDSENNVEVFISDDSQQSVSGLSMPVSSIHN 540
Query: 541 EPNQRPDFSSVNSWNDEAPLQVQASEVLLLPYKEESSTAEVAKEDDQASSSILGCAQEDF 600
EPNQRPDFSSVNSW+DEAPLQVQASEVLLLPYKEESS+AEV KEDDQASSSILGCAQEDF
Sbjct: 541 EPNQRPDFSSVNSWDDEAPLQVQASEVLLLPYKEESSSAEVVKEDDQASSSILGCAQEDF 600
Query: 601 DGFGLGDMFDEPEIPIGPIGRPSTSNEVAESSFNSESDPEEVDNTDAPVSVESCLTFFTK 660
DGFGLGDMFDEPEIPIGPIGRPSTSNEVAESSFNSESDPEEVDNTDAPVSVESCLTFFTK
Sbjct: 601 DGFGLGDMFDEPEIPIGPIGRPSTSNEVAESSFNSESDPEEVDNTDAPVSVESCLTFFTK 660
Query: 661 PELLSNENGYNCEKCSKRLQQQRLEMKKQSKVACKAVANGCQTAVGGDISSCNEDSSVEV 720
PELLSNENGYNCEKCSKRLQQQRLEMKKQSKVACKAVANGCQTAVGGDISSCNE+SSVEV
Sbjct: 661 PELLSNENGYNCEKCSKRLQQQRLEMKKQSKVACKAVANGCQTAVGGDISSCNENSSVEV 720
Query: 721 KNKRNMNLTNGSISYSSGESSNLKKNVDCSSQDCSKPVNCQKSKTDPPVLDEDEAKVDKD 780
KN+RN NLTNGSI Y+SGESSNLKKN DCSSQDC+KPVNCQ KTDP VLDEDEAKVDKD
Sbjct: 721 KNQRNTNLTNGSIIYNSGESSNLKKNADCSSQDCTKPVNCQIGKTDPLVLDEDEAKVDKD 780
Query: 781 MNPGLSRSSGCNNTRNQENSDDKSSCSLPNDEPAKTNIEHLSSHLAVGNQSEKSEDGEMD 840
MNPGLS SSGC+NT NQENSDDKSS SLPNDE AKT IEHLSSHL+VGNQSE+SEDGEMD
Sbjct: 781 MNPGLSCSSGCSNTCNQENSDDKSS-SLPNDEHAKTKIEHLSSHLSVGNQSEESEDGEMD 840
Query: 841 SDSTIVNVKRDATKRFLIHKAPPILTIHMKRFSPDARGRYSKLNGHVRFKETIDLKPYLD 900
SD T+VNVKRDATKRFLIHKAPPILTIHMKRF+PDARGRYSKLNGHVRFKETIDLKPY+D
Sbjct: 841 SDPTVVNVKRDATKRFLIHKAPPILTIHMKRFTPDARGRYSKLNGHVRFKETIDLKPYVD 900
Query: 901 TRCADRDKCSYRLVGVVEHSGSMRGGHYVAYVRGGNRKRSSGEAEEDASVWYYASDAVVD 960
TRCADRDKC+YRLVGVVEHSGSMRGGHYVAYVRGGNRKRSSGEAEEDASVWYYASDAVV+
Sbjct: 901 TRCADRDKCNYRLVGVVEHSGSMRGGHYVAYVRGGNRKRSSGEAEEDASVWYYASDAVVN 960
Query: 961 EVTLDRVLGCEAYILFYEIT 981
EVTLDRVLGCEAYILFYEIT
Sbjct: 961 EVTLDRVLGCEAYILFYEIT 979
BLAST of Cucsa.256480 vs. NCBI nr
Match:
gi|1009140072|ref|XP_015887452.1| (PREDICTED: ubiquitin carboxyl-terminal hydrolase 2 [Ziziphus jujuba])
HSP 1 Score: 973.0 bits (2514), Expect = 3.9e-280
Identity = 529/998 (53.01%), Postives = 682/998 (68.34%), Query Frame = 1
Query: 1 MGKKVKKKTNRAPPKEKRVSSSSPRKVPQPSNTTVETADEEISIVKEKSQCGHLDKCFNL 60
MGKKVKKK+ RAP KEKRV++ + +KVPQPSN VE AD+ +S+VKEK C H++K +
Sbjct: 1 MGKKVKKKS-RAPQKEKRVTNHTSKKVPQPSNPNVEPADDGVSVVKEKKPCPHIEKGVDY 60
Query: 61 NELSSKLGSAEPISCEDCQDSSADRRGGRGKARHGKKKGGTSVDVKTDAKAIWICLQCGH 120
++LS+K EP+ CEDC++ DRR +GK +HGKKKG SVD K++ KAIW+CL+CGH
Sbjct: 61 SKLSAKFALPEPVRCEDCREGVVDRRAAKGKGKHGKKKGSGSVDSKSELKAIWVCLECGH 120
Query: 121 YACGGIGLPTNSQSHAVRHVRQTRHPVVIQFENPQLRWCFSCNTLLPVEKTEENGEQKDS 180
++CGGIGLPT+SQ HAVRHVRQTRHP+VI FE PQLRWCF CNTL+ EK EENGE KD
Sbjct: 121 FSCGGIGLPTSSQCHAVRHVRQTRHPLVIHFEKPQLRWCFPCNTLVTTEKVEENGETKDV 180
Query: 181 LSNVVKLIRDR--SMESTHVDVENTRYTSSEVTAATKPESSVLSDLDRRNQYIVKGLINL 240
S++VKLI+ + + V+VE+ + S VT K S+V SDLD Y+V+GL+NL
Sbjct: 181 FSDIVKLIKGHVSQTQESAVNVEDVWFGSGSVTTEIKSVSNVTSDLDGNAGYMVRGLVNL 240
Query: 241 GNTCFFNSILQNLLAIDMLRDHFVKLEECVGPLTIALKKIFIEARTESRMKSSINPRSVF 300
GNTCFFNSI+QNLLA+D LRD+F+KL+ GPLTIALKK+F+E + E+ +K+ +NPRS F
Sbjct: 241 GNTCFFNSIMQNLLAMDRLRDYFLKLDLSAGPLTIALKKLFVETKPEAGVKNVVNPRSFF 300
Query: 301 GCISSKAPQFKGYEQHDSHELLRVLLDALSSEELTSRKMTNSKEE-RISGNPTPTFVDEM 360
GC+ +KAPQF+GY+QHDSHELLR LLD LS+EEL RK NS EE S +FV+ +
Sbjct: 301 GCVCTKAPQFRGYQQHDSHELLRCLLDGLSTEELGFRKQMNSSEENENSSKGASSFVEAV 360
Query: 361 FGGQISSAVCCKECGHTSTVYEPFLDLSLPVPMKKPLAKKVQPVSRAKKTKVPPKRNGKT 420
FGGQ+SS V C ECGH+STVYEPFLDLSLPVP KKP +KK QP SRAKKTK+PPK++GK
Sbjct: 361 FGGQVSSTVRCTECGHSSTVYEPFLDLSLPVPTKKPPSKKAQPTSRAKKTKLPPKKSGKN 420
Query: 421 IPKTNKVSDIVPIQIASVPSSSNESSL-PSEASASSTTTIMEKTSTSQNVSDAKESGKEI 480
PK K +D + S PS+SNE S P S +T EK S QN S +ES E+
Sbjct: 421 RPKLYKSADSI-----SAPSTSNEVSCEPQSGSGGPSTVAEEKESVLQNFSAVQESVSEL 480
Query: 481 SVENGGECAS---DNLTWMDFLEPE-------LNADNCDISTTQDSENNIEVFISDNSQQ 540
++ E A D+ TWMD+L+ E L + N DIS QD EN + S+ Q
Sbjct: 481 VFQDAAEQAPALLDDSTWMDYLDAETVTDGNNLTSQNTDISNVQDLENK-DALKSEVPIQ 540
Query: 541 SVSGLSMPVSSLHSEPNQRPDFSSVNSWNDEAPLQVQASEVLLLPYKEESSTAEVAKEDD 600
S S V SL+ E + +P+ SSVNSW D+ PLQVQ+SEVLLLPY E S T E E+
Sbjct: 541 CGSESSTQVFSLNEEVDVKPETSSVNSWEDDVPLQVQSSEVLLLPYLETSMTME--PEEV 600
Query: 601 QASSSILGCAQEDFDGFGLGDMFDEPEIPIGPIGRPSTSNEVAESSF----NSESDPEEV 660
+ASSS +G QEDF+GF GD+F+EPE+ GP+ PS +EVAE+ F +SESDP+EV
Sbjct: 601 EASSSNVGGGQEDFEGF--GDLFNEPEVAAGPVAGPSLGDEVAETGFMAGNSSESDPDEV 660
Query: 661 DNTDAPVSVESCLTFFTKPELLSNENGYNCEKCSKRLQQQRLE-MKKQSKVACKAVANGC 720
D++D+PVSVESCLT FTKPELLSN+N ++CE CSK LQQQ+LE MKKQ K A K++ NGC
Sbjct: 661 DDSDSPVSVESCLTHFTKPELLSNDNAWHCENCSKTLQQQKLEAMKKQLKTAPKSLTNGC 720
Query: 721 QTAVGGDISSCNEDSSVEVKNKRNMNLTNGSISYSSGESSNLKK-NVDCSSQDCSKPVNC 780
+ D+ + S +V+ NL+NG+I +++ KK + CSSQ+ SK +
Sbjct: 721 VARIQSDMILDKDLSPADVR-----NLSNGNIK----DAAEFKKPEIICSSQN-SKTIEN 780
Query: 781 QKSKTDPPVLDEDEAKVDKDMNPGLSRSSGCNNTRNQENSDDKSSCSLPNDEPAKTNIEH 840
++ + P++ E + + N +S C NT +QE+ D++ S ++ + N H
Sbjct: 781 GQTDENNPLVSRGEDGIQEQSN-----ASDCYNT-SQESFGDQAIDSCVDESSSPGN--H 840
Query: 841 LSSHLAVGNQSEKSEDGEMDSDSTIVNVKRDATKRFLIHKAPPILTIHMKRFSPDARGRY 900
S+ + S K IV VKRDATKR I+KAPPILTIH+KRFS DARGR
Sbjct: 841 GSAEILNDEISPK-----------IVKVKRDATKRVFINKAPPILTIHLKRFSQDARGRL 900
Query: 901 SKLNGHVRFKETIDLKPYLDTRCADRDKCSYRLVGVVEHSGSMRGGHYVAYVRGGNRKRS 960
SKLNGHV+F+E IDLKPY+D RC D +K YRLVGVVEH G+MRGGHYVA++RG +
Sbjct: 901 SKLNGHVKFREVIDLKPYMDARCIDEEKYDYRLVGVVEHLGTMRGGHYVAFIRGAEKSGG 958
Query: 961 SGEAEEDASVWYYASDAVVDEVTLDRVLGCEAYILFYE 979
+ E S W++ASDA V + +L+ VL CEAYILFYE
Sbjct: 961 NAVKENVGSTWFHASDAYVRQTSLEEVLRCEAYILFYE 958
BLAST of Cucsa.256480 vs. NCBI nr
Match:
gi|657977297|ref|XP_008380556.1| (PREDICTED: ubiquitin carboxyl-terminal hydrolase 2 [Malus domestica])
HSP 1 Score: 948.7 bits (2451), Expect = 7.8e-273
Identity = 516/1042 (49.52%), Postives = 680/1042 (65.26%), Query Frame = 1
Query: 1 MGKKVKKKTNRAPPKEKRVSSSSPRKVPQPSNTTVETADEEISIVKEKSQCGHLDKCFNL 60
MGKKVKKK RAPP+EK V++ SP+K PQPSN +V+ D+ +S+ KE+ C H+DK +L
Sbjct: 1 MGKKVKKKA-RAPPREKLVAADSPKKAPQPSNPSVKDGDDGVSVAKERRPCPHVDKGVDL 60
Query: 61 NELSSKLGSAEPISCEDCQDSSADRRGGRGKARHGKKKGGTSVDVKTDAKAIWICLQCGH 120
++LS+K+GS+ P+ CEDC++ + DRRGG+GK +HGKKKG +SVD K+++KAIW+CL+CGH
Sbjct: 61 DKLSAKIGSSGPVRCEDCREGALDRRGGKGKGKHGKKKGSSSVDSKSESKAIWVCLECGH 120
Query: 121 YACGGIGLPTNSQSHAVRHVRQTRHPVVIQFENPQLRWCFSCNTLLPVEKTEENGEQKDS 180
Y+CGGIGLPT Q HAVRH RQTRHP+VI FE PQ+RWCF CN L+ +EKTEE+GEQKD
Sbjct: 121 YSCGGIGLPTTPQCHAVRHARQTRHPIVIHFEKPQMRWCFPCNMLITIEKTEEDGEQKDV 180
Query: 181 LSNVVKLIRDRSMESTHVDVENTRYTSSEVTAATKPESSVLSDLDRRNQYIVKGLINLGN 240
++VVKLI+ S E + V+ E+ + S VT+ K S++ S LD R Y+V+GL+NLGN
Sbjct: 181 FADVVKLIKGHSSEGSSVNAEDVWFASGSVTSEIKSASNISSGLDGRGGYMVRGLVNLGN 240
Query: 241 TCFFNSILQNLLAIDMLRDHFVKLEECVGPLTIALKKIFIEARTESRMKSSINPRSVFGC 300
TCFFNS+LQNLLAID LR +F+ L+ G LT++LKK+F EA+ E+ +K+ INPRS+FGC
Sbjct: 241 TCFFNSVLQNLLAIDRLRGYFLNLDASTGALTVSLKKLFTEAKPEAGLKNVINPRSLFGC 300
Query: 301 ISSKAPQFKGYEQHDSHELLRVLLDALSSEELTSRKMTNSKEERISGNPT---PTFVDEM 360
+ SKA QF+GY+Q DSHELLR LLD L +EEL+ RK NS +E +GNP+ PTFV+
Sbjct: 301 VCSKASQFRGYQQQDSHELLRCLLDGLCTEELSMRKRINSSQE--NGNPSISGPTFVEAA 360
Query: 361 FGGQISSAVCCKECGHTSTVYEPFLDLSLPVPMKKPLAKKVQPVSRAKKTKVPPKRN--- 420
FGGQ SS VCC ECGH+S VYE FLDLSL VP +K +K QP SRAKKTK+PPKR+
Sbjct: 361 FGGQTSSTVCCVECGHSSIVYESFLDLSLSVPTRKSPSKAAQPTSRAKKTKLPPKRSGKL 420
Query: 421 ----------------------------------GKTIPKTNKVSDIVPIQIASVPSSSN 480
T P + ++V +++V S N
Sbjct: 421 RPKNNKEKNSVPSPSVPTPSTSGEVSDHSDQPQSSSTDPNVAEQKELVVNSLSAVQESPN 480
Query: 481 ESSLPSEASASSTTTIMEKTSTSQNVSDAKESGKEISVENGGECAS---DNLTWMDFLEP 540
E E +A T+T+++ S + E+ E S D+ +W+D+L+
Sbjct: 481 EQVC--EDAAEQTSTLLDDCSGLDYLDSGNMVDDNEVCEDAAEQTSTLLDDCSWLDYLDS 540
Query: 541 -------ELNADNCDISTTQDSENNIEVFISDNSQQSVSGLSMPVSSLHSEPNQRPDFSS 600
+ ++ N D+S QDSEN ++D S QS S S V +L+ EPN PD SS
Sbjct: 541 GNMLDDNDFSSQNNDVSIVQDSENKSS-HLNDVSLQSGSESSNQVFTLNKEPNVEPDRSS 600
Query: 601 VNSWNDEAPLQVQASEVLLLPYKEE-SSTAEVAKEDDQASSSILGCAQEDFDGFGLGDMF 660
VNSW +E PLQVQ SEVLLLPYK E S+T E+ + +ASSS++GC Q+DF G GD+F
Sbjct: 601 VNSWEEELPLQVQDSEVLLLPYKAECSTTEEIMGGEGEASSSVVGCGQDDF---GFGDLF 660
Query: 661 DEPEIPIGPIGRPSTSNEVAESSF-NSESDPEEVDNTDAPVSVESCLTFFTKPELLSNEN 720
+EPE+ GP RPS ++S SESDP+EVD+TD+PVSVESCLT F +PELL+NEN
Sbjct: 661 NEPEV-YGPPTRPSVGEGGTDTSIIASESDPDEVDDTDSPVSVESCLTHFIRPELLANEN 720
Query: 721 GYNCEKCSKRLQQQRLEMKKQSKVACKAVANGCQTAVGGDISSCNEDSSVEVKNKRNMNL 780
++CE CSK LQ+QRLE KKQ K A K + NGC+ D S N + +V+ N N+
Sbjct: 721 AWHCENCSKILQRQRLEGKKQGKSAAKVLINGCEARTQSDSLSLNTGPA-DVRRLGNGNV 780
Query: 781 TNGSISYSSGESSNLKKN-VDCSSQDCSKPVNCQKSKTDPPVLDEDEAKVDKDMNPGLSR 840
+ + GE+ L ++C +Q+CS N + K++ V + +D P S
Sbjct: 781 KSNTGCNQFGENFVLHDGKINCWNQNCSAIENGRSDKSNSVVCQRQDEM--EDAPPVQSN 840
Query: 841 SSGCNNTRNQEN---------SDDKSSCSLPNDEPAKTNIEHLSSHLAVGNQSEKSEDGE 900
+S C N + E+ +D+ S S D +TN L H +SE+SED E
Sbjct: 841 TSDCTNACSLESFSDQVIDSRADESRSASFTCDIVPQTNYGILDGH----RESEESEDEE 900
Query: 901 MDSDSTIVNVKRDATKRFLIHKAPPILTIHMKRFSPDARGRYSKLNGHVRFKETIDLKPY 960
++S VNVKR+ATKR+LI++APPILTIH+KRFS DARGR SKLNGH+ F+E IDL+PY
Sbjct: 901 VNSKR--VNVKRNATKRYLINRAPPILTIHLKRFSQDARGRLSKLNGHISFQEKIDLRPY 960
Query: 961 LDTRCADRDKCSYRLVGVVEHSGSMRGGHYVAYVRGGNRKRSSGEAEEDASVWYYASDAV 981
+D+RC D +K Y+L+GVVEHSG+MRGGHYVAYVRGG R R E E WYYASDA
Sbjct: 961 MDSRCTDGEKYEYQLLGVVEHSGTMRGGHYVAYVRGGERSRGKTERENTGHAWYYASDAH 1020
BLAST of Cucsa.256480 vs. NCBI nr
Match:
gi|645235963|ref|XP_008224512.1| (PREDICTED: LOW QUALITY PROTEIN: ubiquitin carboxyl-terminal hydrolase 2 [Prunus mume])
HSP 1 Score: 939.1 bits (2426), Expect = 6.2e-270
Identity = 502/1001 (50.15%), Postives = 673/1001 (67.23%), Query Frame = 1
Query: 1 MGKKVKKKTNRAPPKEKRVSSSSPRKVPQPSNTTVETADEEISIVKEKSQCGHLDKCFNL 60
MGKKVKKK AP K+K +++ SP+K PQPSN +V+ D+ +S+ KEK C H+DK +L
Sbjct: 1 MGKKVKKKARAAPQKDKWIAADSPKKAPQPSNPSVKNGDDAVSVPKEKKPCPHVDKGVDL 60
Query: 61 NELSSKLGSAEPISCEDCQDSSADRRGGRGKARHGKKKGGTSVDVKTDAKAIWICLQCGH 120
N+LS+K+GS+E + CEDC++ + DRRGG+GK +HGKKKG +SVD K+++KAIW+CL+CGH
Sbjct: 61 NKLSAKIGSSELVRCEDCREVALDRRGGKGKGKHGKKKGSSSVDSKSESKAIWVCLECGH 120
Query: 121 YACGGIGLPTNSQSHAVRHVRQTRHPVVIQFENPQLRWCFSCNTLLPVEKTEENGEQKDS 180
Y+CGG+GLPT Q HA+RH RQTRHP+VI FENPQLRWCFSC+ L+ ++K EEN EQKD
Sbjct: 121 YSCGGVGLPTTPQCHALRHARQTRHPLVIHFENPQLRWCFSCSMLIKIDKMEENSEQKDV 180
Query: 181 LSNVVKLIRDRSMESTHVDVENTRYTSSEVTAATKPESSVLSDLDRRNQYIVKGLINLGN 240
S+VVKLI+ S E V+VE+ + + VT+ K +++ SDLD R Y+V+GL+NLGN
Sbjct: 181 FSDVVKLIKGHSSEEPSVNVEDVWFGNGSVTSDIKSANNISSDLDGRGGYMVRGLVNLGN 240
Query: 241 TCFFNSILQNLLAIDMLRDHFVKLEECVGPLTIALKKIFIEARTESRMKSSINPRSVFGC 300
TCFFNS+LQN+LAID LR +F+ ++ G LTI+LKK+F E + E+ ++ INPR+ FGC
Sbjct: 241 TCFFNSVLQNILAIDRLRGYFLNVDAFSGALTISLKKLFTETKPEAGFRNVINPRAFFGC 300
Query: 301 ISSKAPQFKGYEQHDSHELLRVLLDALSSEELTSRK-MTNSKEERISGNPTPTFVDEMFG 360
+ SKAPQF+GY+Q DSHELLR LLD L +EEL+ RK +++S+E S NP PTFVD +FG
Sbjct: 301 VCSKAPQFRGYQQQDSHELLRCLLDGLCTEELSMRKRISSSRENGNSSNPGPTFVDAVFG 360
Query: 361 GQISSAVCCKECGHTSTVYEPFLDLSLPVPMKKPLAKKVQPVSRAKKTKVPPKRNGKTIP 420
GQISS V C ECGH+STVYE FLDLSLPVP +K K QP SRAKKTK+PPKR+GK
Sbjct: 361 GQISSTVSCVECGHSSTVYESFLDLSLPVPTRKCPPKTAQPTSRAKKTKLPPKRSGKVRS 420
Query: 421 KTNKVSDIVPIQIASVPSSSNESSLPSEASASSTTTIMEKTSTSQNVSDAKESGKEISVE 480
K NK P + PS+ +E S ++ ++ + + +N+S +ES E E
Sbjct: 421 KINKDKSSAPSSSVATPSTRSEVSSQPQSGSTDPDVVEQWGLVMKNLSAVQESENEQVFE 480
Query: 481 NGGECAS---DNLTWMDFL-------EPELNADNCDISTTQDSENNIEVFISDNSQQSVS 540
+ E S ++ TW+D+L + + + N D S QDSEN + ++D Q S
Sbjct: 481 DAAEQTSTLLNDCTWLDYLDMGNMSDDNDFVSQNNDASNVQDSEN--KNALNDILLQPDS 540
Query: 541 GLSMPVSSLHSEPNQRPDFSSVNSWNDEAPLQVQASEVLLLPYKEESS-TAEVAKEDDQA 600
VS+L+ EPN +PDFS VN W +E PLQVQ SEVLLLPYKEE S T E+ +D+A
Sbjct: 541 ESGNQVSTLNGEPNVKPDFSLVNPWEEELPLQVQGSEVLLLPYKEECSVTEEIIGREDEA 600
Query: 601 SSSILGCAQEDFDGFGLGDMFDEPEIPIGPIGRPSTSNEVAESSF-NSESDPEEVDNTDA 660
SSS++G Q++FDGF GD+F+EPE+ GP RPS E+ F SESDP+EVD++D+
Sbjct: 601 SSSVVGGGQDEFDGF--GDLFNEPEVAAGPTARPSVGEGGTETGFVASESDPDEVDDSDS 660
Query: 661 PVSVESCLTFFTKPELLSNENGYNCEKCSKRLQQQRLEMKKQSKVACKAVANGCQTAVGG 720
PVSVESCL F KPELL+NEN ++CE CS+ LQ+QRLE+KKQ K + + + NGC+T
Sbjct: 661 PVSVESCLAHFIKPELLANENAWHCENCSRTLQRQRLEVKKQVKSSAQILINGCETRAES 720
Query: 721 DISSCNED-SSVEVKNKRNMNLTNGSISYSSGESSNLKKNVDCSSQDCSKPVNCQKSKTD 780
D S N +V+ NL+NG+++ S+G ++ +++ +CS N + K +
Sbjct: 721 DSLSLNMGLCPADVR-----NLSNGNLNSSTG-CNHFGEDLHDGKINCSSIENGRSDKLN 780
Query: 781 PPVLDEDEAKVD-KDMNPGLSRSSGCNNTRNQENSDDKS--SCSLPNDEP-----AKTNI 840
V ++E + KD P S +S CNNT ++E+ D++ SC+ DEP N+
Sbjct: 781 AAVRQQEEGNNEMKDALPVQSNTSDCNNTCSRESYIDQAIESCA---DEPRTAGCTSDNV 840
Query: 841 EHLSSHLAVG-NQSEKSEDGEMDSDSTIVNVKRDATKRFLIHKAPPILTIHMKRFSPDAR 900
S + G ++SE+SED E+ +S V VKRDATKR L
Sbjct: 841 PQTYSGILDGKHESEESEDEEI--NSKCVKVKRDATKRVL-------------------- 900
Query: 901 GRYSKLNGHVRFKETIDLKPYLDTRCADRDKCSYRLVGVVEHSGSMRGGHYVAYVRGGNR 960
R SKLNGHV F+E IDL+PY+D+R D +K YRL+GVVEHSG+MRGGHYVAYVRGG R
Sbjct: 901 -RLSKLNGHVSFREKIDLRPYMDSRSTDGEKYEYRLIGVVEHSGTMRGGHYVAYVRGGER 960
Query: 961 KRSSGEAEEDASVWYYASDAVVDEVTLDRVLGCEAYILFYE 979
R E E + +VWYYASDA V +V+LD VL CEAYILFYE
Sbjct: 961 SRGKAEKENNGNVWYYASDAHVRQVSLDEVLHCEAYILFYE 965
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| UBP2_ARATH | 1.5e-151 | 39.70 | Ubiquitin carboxyl-terminal hydrolase 2 OS=Arabidopsis thaliana GN=UBP2 PE=1 SV=... | [more] |
| UBP1_ARATH | 2.0e-103 | 38.57 | Ubiquitin carboxyl-terminal hydrolase 1 OS=Arabidopsis thaliana GN=UBP1 PE=1 SV=... | [more] |
| UBP16_RAT | 4.7e-44 | 23.80 | Ubiquitin carboxyl-terminal hydrolase 16 OS=Rattus norvegicus GN=Usp16 PE=1 SV=2 | [more] |
| UBP16_MOUSE | 1.8e-43 | 24.40 | Ubiquitin carboxyl-terminal hydrolase 16 OS=Mus musculus GN=Usp16 PE=1 SV=2 | [more] |
| UBP16_MACFA | 4.0e-43 | 24.78 | Ubiquitin carboxyl-terminal hydrolase 16 OS=Macaca fascicularis GN=USP16 PE=2 SV... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A0A0L9E4_CUCSA | 0.0e+00 | 100.00 | Uncharacterized protein OS=Cucumis sativus GN=Csa_3G130860 PE=3 SV=1 | [more] |
| A0A067L5H9_JATCU | 5.6e-270 | 51.81 | Uncharacterized protein OS=Jatropha curcas GN=JCGZ_01121 PE=3 SV=1 | [more] |
| B9RWG7_RICCO | 1.9e-262 | 49.80 | Putative uncharacterized protein OS=Ricinus communis GN=RCOM_1019320 PE=3 SV=1 | [more] |
| A0A061EJT6_THECC | 1.6e-261 | 49.47 | Ubiquitin carboxyl-terminal hydrolase, putative isoform 1 OS=Theobroma cacao GN=... | [more] |
| A0A0D2RE60_GOSRA | 1.6e-253 | 46.76 | Uncharacterized protein OS=Gossypium raimondii GN=B456_005G130100 PE=3 SV=1 | [more] |
