Cucsa.255380 (gene) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.255380
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	Phosphoenolpyruvate carboxylase kinase 4
Location	scaffold02229 : 3398415 .. 3399929 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TCCTCCTCACTTCACGTTTTTTACTTTCTTCCATAAATATTCAACCAAACCAAACGCTTTCTAATTAGGTTCCGTACAGTTCCACACTCGAAAACCAAAAAGAGAGGAAAGCAAAAGAAAAACAGAGTAATGAGCGACGGATTTAAGAGAGACTATCAAGTAATTGAAGAGCTTGGCCGGGGAAGATTTGGTACCGTTTTCCGATGTATTTCTAAATCTTGCGGTGGAAACTACGCCGTCAAAATCATCGACAAGCGTCGGATCTCGGCCGGTGATTCACTCGACGCGGAATGTCTTGTTAACGAGACGAAGATTCTTCATCTCCTTTACCCTCACCCTCACATTCTCGCGTTGCATAATCTCTACGAAGACGAATCTCACCTTCATATGGTTCTTGATCTTTGTTCTTCTTCCGATCTCCACCGTCGAATCACACTTCAGGTTTTTTCCGAAGCTGAGGCGGCTAGGATTATGTCACAACTGATGCACGCGGTGGCTCATTGTCACCGTCACGGCGTTGCGCATCGTGATATTAAACCAGACAATATTCTGTTCGACGAGTGGGATTCGGTGAAATTGGCGGATTTCGGATCGACGGAAATGTTTAAGCAGGGAGAAGAGTCGATGAGCGGTGTCGTCGGAACGCCGTACTACGTAGCGCCAGAAGTTCTAGCCGGAAAAGACTACGGCGAAAAGGTTGACGTCTGGAGCGCCGGCGTGGTTTTGTATGTTATGTTAGCTGGTTTTCCTCCGTTTCATGGTGAATCGGTTGTTGAGATCTTTCACGCGGTGTTACGAGCGAATTTGAGATTTCCGTCCAGAGTTTTCCATTCTGTATCTCCCTCGGCAAAAGACTTGCTTCGTAAAATGCTATGTAAAGACGTTTCCAGAAGAATTTCCGCGGAACAAGTCCTCAGTGAGTATATAGCTATTTCCCTTCAATTATCTCTAAATATTTCCCTTTTCGCCGTCTTTTATCAATCTTCCACTGAACACCATTCTTCTTCATCTCGAAAAATCAAACCCTCTTCAAAAAAAAAAAAAAACAACGAAACCCCTAATTTGTGCCCTGAAACAGAATTAATTCCTTTAAACCACCCCAAATCTTTGAGATCTTATCAATGTATTCGATTCAAATACACTACGCAAACAAATCCAAGCCTTAATTTAGTTTGATTTGATATATATATATATATAATATTATGTAGCATTATATTATTTTCTTCCGCTTAGTAATTCTTCCGTTTTTTGTTTGGCGGAATCAGGACATCCATGGATAACAAGGTACGCAGAAAACATGGGAGAAACGGAAGTAGGGTGAAATTTTGCAATAATTGAAGGAAGAAAAGAAGAGAATATATTATGATTGAGCCTCATCTTCTTCCATTTTCTTTTTTGTATAGAGGGTTTCGACAGTTGTAATTATAATGAGGGTATTATGATATATAAAGCAGTTTAATTAGTGAAAGAGTTTGTATAAACTGTTTCAAACTTCTCCGTTGATTTCTATATGCA

mRNA sequence

TCCTCCTCACTTCACGTTTTTTACTTTCTTCCATAAATATTCAACCAAACCAAACGCTTTCTAATTAGGTTCCGTACAGTTCCACACTCGAAAACCAAAAAGAGAGGAAAGCAAAAGAAAAACAGAGTAATGAGCGACGGATTTAAGAGAGACTATCAAGTAATTGAAGAGCTTGGCCGGGGAAGATTTGGTACCGTTTTCCGATGTATTTCTAAATCTTGCGGTGGAAACTACGCCGTCAAAATCATCGACAAGCGTCGGATCTCGGCCGGTGATTCACTCGACGCGGAATGTCTTGTTAACGAGACGAAGATTCTTCATCTCCTTTACCCTCACCCTCACATTCTCGCGTTGCATAATCTCTACGAAGACGAATCTCACCTTCATATGGTTCTTGATCTTTGTTCTTCTTCCGATCTCCACCGTCGAATCACACTTCAGGTTTTTTCCGAAGCTGAGGCGGCTAGGATTATGTCACAACTGATGCACGCGGTGGCTCATTGTCACCGTCACGGCGTTGCGCATCGTGATATTAAACCAGACAATATTCTGTTCGACGAGTGGGATTCGGTGAAATTGGCGGATTTCGGATCGACGGAAATGTTTAAGCAGGGAGAAGAGTCGATGAGCGGTGTCGTCGGAACGCCGTACTACGTAGCGCCAGAAGTTCTAGCCGGAAAAGACTACGGCGAAAAGGTTGACGTCTGGAGCGCCGGCGTGGTTTTGTATGTTATGTTAGCTGGTTTTCCTCCGTTTCATGGTGAATCGGTTGTTGAGATCTTTCACGCGGTGTTACGAGCGAATTTGAGATTTCCGTCCAGAGTTTTCCATTCTGTATCTCCCTCGGCAAAAGACTTGCTTCGTAAAATGCTATGTAAAGACGTTTCCAGAAGAATTTCCGCGGAACAAGTCCTCAGACATCCATGGATAACAAGGTACGCAGAAAACATGGGAGAAACGGAAGTAGGGTGAAATTTTGCAATAATTGAAGGAAGAAAAGAAGAGAATATATTATGATTGAGCCTCATCTTCTTCCATTTTCTTTTTTGTATAGAGGGTTTCGACAGTTGTAATTATAATGAGGGTATTATGATATATAAAGCAGTTTAATTAGTGAAAGAGTTTGTATAAACTGTTTCAAACTTCTCCGTTGATTTCTATATGCA

Coding sequence (CDS)

ATGAGCGACGGATTTAAGAGAGACTATCAAGTAATTGAAGAGCTTGGCCGGGGAAGATTTGGTACCGTTTTCCGATGTATTTCTAAATCTTGCGGTGGAAACTACGCCGTCAAAATCATCGACAAGCGTCGGATCTCGGCCGGTGATTCACTCGACGCGGAATGTCTTGTTAACGAGACGAAGATTCTTCATCTCCTTTACCCTCACCCTCACATTCTCGCGTTGCATAATCTCTACGAAGACGAATCTCACCTTCATATGGTTCTTGATCTTTGTTCTTCTTCCGATCTCCACCGTCGAATCACACTTCAGGTTTTTTCCGAAGCTGAGGCGGCTAGGATTATGTCACAACTGATGCACGCGGTGGCTCATTGTCACCGTCACGGCGTTGCGCATCGTGATATTAAACCAGACAATATTCTGTTCGACGAGTGGGATTCGGTGAAATTGGCGGATTTCGGATCGACGGAAATGTTTAAGCAGGGAGAAGAGTCGATGAGCGGTGTCGTCGGAACGCCGTACTACGTAGCGCCAGAAGTTCTAGCCGGAAAAGACTACGGCGAAAAGGTTGACGTCTGGAGCGCCGGCGTGGTTTTGTATGTTATGTTAGCTGGTTTTCCTCCGTTTCATGGTGAATCGGTTGTTGAGATCTTTCACGCGGTGTTACGAGCGAATTTGAGATTTCCGTCCAGAGTTTTCCATTCTGTATCTCCCTCGGCAAAAGACTTGCTTCGTAAAATGCTATGTAAAGACGTTTCCAGAAGAATTTCCGCGGAACAAGTCCTCAGACATCCATGGATAACAAGGTACGCAGAAAACATGGGAGAAACGGAAGTAGGGTGA

Protein sequence

MSDGFKRDYQVIEELGRGRFGTVFRCISKSCGGNYAVKIIDKRRISAGDSLDAECLVNETKILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLQVFSEAEAARIMSQLMHAVAHCHRHGVAHRDIKPDNILFDEWDSVKLADFGSTEMFKQGEESMSGVVGTPYYVAPEVLAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPSAKDLLRKMLCKDVSRRISAEQVLRHPWITRYAENMGETEVG*

Homology

[Close]

BLAST of Cucsa.255380 vs. Swiss-Prot
Match: PPCK1_ARATH (Phosphoenolpyruvate carboxylase kinase 1 OS=Arabidopsis thaliana GN=PPCK1 PE=1 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 1.0e-70
Identity = 141/257 (54.86%), Postives = 179/257 (69.65%), Query Frame = 1


Query: 9   YQVIEELGRGRFGTVFRCISKSCGGNYAVKIIDKRRISAGDSLDAECLVNETKILHLLYP 68
           YQ+ EE+GRGRFGTV R  + + G  +A K IDK  +S  D LD  CL NE K++ LL  
Sbjct: 15  YQICEEIGRGRFGTVSRVYAPATGDFFACKTIDKASLS--DDLDRACLDNEPKLMALLSY 74

Query: 69  HPHILALHNLYEDESHLHMVLDLC--SSSDLHRRITLQVFSEAEAARIMSQLMHAVAHCH 128
           HP+I+ +H+L + +S L + ++L   S S   R ++   F E + A    Q++ A++HCH
Sbjct: 75  HPNIVQIHDLIDTDSTLSIFMELVHPSVSIYDRLVSSGTFFEPQTASFAKQILQALSHCH 134

Query: 129 RHGVAHRDIKPDNILFD-EWDSVKLADFGSTEMFKQGEESMSGVVGTPYYVAPEVLAGKD 188
           R+GV HRDIKP+NIL D   D+VK+ DFGS     +GE +  GVVGTPYYVAPEVL G  
Sbjct: 135 RYGVVHRDIKPENILVDLRNDTVKICDFGSGIWLGEGETT-EGVVGTPYYVAPEVLMGYS 194

Query: 189 YGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPSAKDLLR 248
           YGEKVD+WSAGVVLY MLAG PPF+GE+  EIF AVLR NLRFP+++F  VS  AKD LR
Sbjct: 195 YGEKVDLWSAGVVLYTMLAGTPPFYGETAEEIFEAVLRGNLRFPTKIFRGVSSMAKDFLR 254

Query: 249 KMLCKDVSRRISAEQVL 263
           K++CKD SRR SAEQ L
Sbjct: 255 KLICKDASRRFSAEQAL 268

[Close]

BLAST of Cucsa.255380 vs. Swiss-Prot
Match: PPCK2_ARATH (Phosphoenolpyruvate carboxylase kinase 2 OS=Arabidopsis thaliana GN=PPCK2 PE=1 SV=2)

HSP 1 Score: 257.7 bits (657), Expect = 1.8e-67
Identity = 136/261 (52.11%), Postives = 179/261 (68.58%), Query Frame = 1


Query: 6   KRDYQVIEELGRGRFGTVFRCISKSCGGNYAVKIIDKRRISAGDSLDAECLVNETKILHL 65
           + +YQ+ +E+GRGRFGT+ RC S +    YA K IDKR +   D+LD EC+  E +I+ +
Sbjct: 8   ENNYQLCDEIGRGRFGTITRCFSPATKEFYACKTIDKRVLI--DALDRECIETEPRIMAM 67

Query: 66  LYPHPHILALHNLYEDESHLHMVLDLCSSS-DLHRRITLQV--FSEAEAARIMSQLMHAV 125
           L PHP+I+ + +LYE E  L +V++L      ++ R+       SE+E+A    Q++ A+
Sbjct: 68  LPPHPNIIRIFDLYETEDSLAIVMELVDPPMTIYDRLISAGGRLSESESASYAKQILSAL 127

Query: 126 AHCHRHGVAHRDIKPDNILFDEWDS-VKLADFGSTEMFKQGEESMSGVVGTPYYVAPEVL 185
           AHCHR  V HRD+KPDN+L D     VKL DFGS      G E+  GVVGTPYYVAPEV+
Sbjct: 128 AHCHRCDVVHRDVKPDNVLVDLVSGGVKLCDFGSAVWL--GGETAEGVVGTPYYVAPEVV 187

Query: 186 AGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPSAK 245
            G+ Y EKVD+WSAGVV+Y MLAG PPF+GE+  +IF ++LR NLRFP + F SVS  AK
Sbjct: 188 MGRKYDEKVDIWSAGVVIYTMLAGEPPFNGETAEDIFESILRGNLRFPPKKFGSVSSEAK 247

Query: 246 DLLRKMLCKDVSRRISAEQVL 263
           DLLRKM+C+DVSRR SAE  L
Sbjct: 248 DLLRKMICRDVSRRFSAEDAL 264

[Close]

BLAST of Cucsa.255380 vs. Swiss-Prot
Match: CDPK2_ARATH (Calcium-dependent protein kinase 2 OS=Arabidopsis thaliana GN=CPK2 PE=1 SV=1)

HSP 1 Score: 211.5 bits (537), Expect = 1.5e-53
Identity = 115/265 (43.40%), Postives = 168/265 (63.40%), Query Frame = 1


Query: 2   SDGFKRDYQVIEELGRGRFGTVFRCISKSCGGNYAVKIIDKRRISAGDSLDAECLVNETK 61
           ++ FK  Y +  +LG+G+FGT F C+ K  G  YA K I KR++   +  D E +  E +
Sbjct: 179 TENFKEFYSLGRKLGQGQFGTTFLCLEKGTGNEYACKSISKRKLLTDE--DVEDVRREIQ 238

Query: 62  ILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLQV-FSEAEAARIMSQLMH 121
           I+H L  HP+++++   YED   +H+V++LCS  +L  RI  +  ++E +AA +   ++ 
Sbjct: 239 IMHHLAGHPNVISIKGAYEDVVAVHLVMELCSGGELFDRIIQRGHYTERKAAELARTIVG 298

Query: 122 AVAHCHRHGVAHRDIKPDNILF---DEWDSVKLADFGSTEMFKQGEESMSGVVGTPYYVA 181
            +  CH  GV HRD+KP+N LF   +E   +K  DFG +  FK  +E  + VVG+PYYVA
Sbjct: 299 VLEACHSLGVMHRDLKPENFLFVSREEDSLLKTIDFGLSMFFKP-DEVFTDVVGSPYYVA 358

Query: 182 PEVLAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVS 241
           PEVL  K YG + DVWSAGV++Y++L+G PPF  E+   IF  VL  +L F S  + S+S
Sbjct: 359 PEVLR-KRYGPESDVWSAGVIVYILLSGVPPFWAETEQGIFEQVLHGDLDFSSDPWPSIS 418

Query: 242 PSAKDLLRKMLCKDVSRRISAEQVL 263
            SAKDL+RKML +D  RR++A QVL
Sbjct: 419 ESAKDLVRKMLVRDPKRRLTAHQVL 439

[Close]

BLAST of Cucsa.255380 vs. Swiss-Prot
Match: CDPK_SOYBN (Calcium-dependent protein kinase SK5 OS=Glycine max PE=1 SV=1)

HSP 1 Score: 209.9 bits (533), Expect = 4.4e-53
Identity = 114/258 (44.19%), Postives = 164/258 (63.57%), Query Frame = 1


Query: 9   YQVIEELGRGRFGTVFRCISKSCGGNYAVKIIDKRRISAGDSLDAECLVNETKILHLLYP 68
           Y+V  +LG+G+FGT F C  ++ GG +A K I KR++   +  D E +  E +I+H L  
Sbjct: 34  YEVGRKLGQGQFGTTFECTRRASGGKFACKSIPKRKLLCKE--DYEDVWREIQIMHHLSE 93

Query: 69  HPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLQV-FSEAEAARIMSQLMHAVAHCHR 128
           H +++ +   YED + +H+V++LC   +L  RI  +  +SE +AAR++  ++  V  CH 
Sbjct: 94  HANVVRIEGTYEDSTAVHLVMELCEGGELFDRIVQKGHYSERQAARLIKTIVEVVEACHS 153

Query: 129 HGVAHRDIKPDNILFDEWDS---VKLADFGSTEMFKQGEESMSGVVGTPYYVAPEVLAGK 188
            GV HRD+KP+N LFD  D    +K  DFG +  +K GE S   VVG+PYYVAPEVL  K
Sbjct: 154 LGVMHRDLKPENFLFDTIDEDAKLKATDFGLSVFYKPGE-SFCDVVGSPYYVAPEVLR-K 213

Query: 189 DYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPSAKDLL 248
            YG + DVWSAGV+LY++L+G PPF  ES   IF  +L   L F S  + S+S SAKDL+
Sbjct: 214 LYGPESDVWSAGVILYILLSGVPPFWAESEPGIFRQILLGKLDFHSEPWPSISDSAKDLI 273

Query: 249 RKMLCKDVSRRISAEQVL 263
           RKML ++   R++A +VL
Sbjct: 274 RKMLDQNPKTRLTAHEVL 287

[Close]

BLAST of Cucsa.255380 vs. Swiss-Prot
Match: CDPK1_ARATH (Calcium-dependent protein kinase 1 OS=Arabidopsis thaliana GN=CPK1 PE=1 SV=1)

HSP 1 Score: 204.5 bits (519), Expect = 1.9e-51
Identity = 111/265 (41.89%), Postives = 167/265 (63.02%), Query Frame = 1


Query: 2   SDGFKRDYQVIEELGRGRFGTVFRCISKSCGGNYAVKIIDKRRISAGDSLDAECLVNETK 61
           ++ FK  Y +  +LG+G+FGT F C+ K+ G  +A K I KR++   +  D E +  E +
Sbjct: 143 TENFKEFYSLGRKLGQGQFGTTFLCVEKTTGKEFACKSIAKRKLLTDE--DVEDVRREIQ 202

Query: 62  ILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLQV-FSEAEAARIMSQLMH 121
           I+H L  HP+++++   YED   +H+V++ C+  +L  RI  +  ++E +AA +   ++ 
Sbjct: 203 IMHHLAGHPNVISIKGAYEDVVAVHLVMECCAGGELFDRIIQRGHYTERKAAELTRTIVG 262

Query: 122 AVAHCHRHGVAHRDIKPDNILF---DEWDSVKLADFGSTEMFKQGEESMSGVVGTPYYVA 181
            V  CH  GV HRD+KP+N LF    E   +K  DFG +  FK  ++  + VVG+PYYVA
Sbjct: 263 VVEACHSLGVMHRDLKPENFLFVSKHEDSLLKTIDFGLSMFFKP-DDVFTDVVGSPYYVA 322

Query: 182 PEVLAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVS 241
           PEVL  K YG + DVWSAGV++Y++L+G PPF  E+   IF  VL  +L F S  + S+S
Sbjct: 323 PEVLR-KRYGPEADVWSAGVIVYILLSGVPPFWAETEQGIFEQVLHGDLDFSSDPWPSIS 382

Query: 242 PSAKDLLRKMLCKDVSRRISAEQVL 263
            SAKDL+RKML +D  +R++A QVL
Sbjct: 383 ESAKDLVRKMLVRDPKKRLTAHQVL 403

[Close]

BLAST of Cucsa.255380 vs. TrEMBL
Match: A0A0A0L743_CUCSA (Phosphoenolpyruvate carboxylase kinase 4 OS=Cucumis sativus GN=Csa_3G135070 PE=3 SV=1)

HSP 1 Score: 532.3 bits (1370), Expect = 4.3e-148
Identity = 262/262 (100.00%), Postives = 262/262 (100.00%), Query Frame = 1


Query: 1   MSDGFKRDYQVIEELGRGRFGTVFRCISKSCGGNYAVKIIDKRRISAGDSLDAECLVNET 60
           MSDGFKRDYQVIEELGRGRFGTVFRCISKSCGGNYAVKIIDKRRISAGDSLDAECLVNET
Sbjct: 1   MSDGFKRDYQVIEELGRGRFGTVFRCISKSCGGNYAVKIIDKRRISAGDSLDAECLVNET 60

Query: 61  KILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLQVFSEAEAARIMSQLMH 120
           KILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLQVFSEAEAARIMSQLMH
Sbjct: 61  KILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLQVFSEAEAARIMSQLMH 120

Query: 121 AVAHCHRHGVAHRDIKPDNILFDEWDSVKLADFGSTEMFKQGEESMSGVVGTPYYVAPEV 180
           AVAHCHRHGVAHRDIKPDNILFDEWDSVKLADFGSTEMFKQGEESMSGVVGTPYYVAPEV
Sbjct: 121 AVAHCHRHGVAHRDIKPDNILFDEWDSVKLADFGSTEMFKQGEESMSGVVGTPYYVAPEV 180

Query: 181 LAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPSA 240
           LAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPSA
Sbjct: 181 LAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPSA 240

Query: 241 KDLLRKMLCKDVSRRISAEQVL 263
           KDLLRKMLCKDVSRRISAEQVL
Sbjct: 241 KDLLRKMLCKDVSRRISAEQVL 262

[Close]

BLAST of Cucsa.255380 vs. TrEMBL
Match: W9RPC3_9ROSA (Phosphoenolpyruvate carboxylase kinase 1 OS=Morus notabilis GN=L484_021421 PE=3 SV=1)

HSP 1 Score: 392.1 bits (1006), Expect = 7.0e-106
Identity = 194/263 (73.76%), Postives = 222/263 (84.41%), Query Frame = 1


Query: 1   MSDGFKRDYQVIEELGRGRFGTVFRCISKSCGGNY-AVKIIDKRRISAGDSLDAECLVNE 60
           MS    RDYQV+EE+GRGRFG V+RC   +  G++ AVK IDKR ISAGDSLDA+CL +E
Sbjct: 1   MSQALNRDYQVLEEIGRGRFGVVYRCSPVTNSGDFFAVKSIDKRLISAGDSLDAQCLFSE 60

Query: 61  TKILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLQVFSEAEAARIMSQLM 120
            KIL  L PHPHI+ LH+LYED+ +LHMVLDLC + DLH R+T +V+SE EAA +M+QLM
Sbjct: 61  PKILRFLSPHPHIINLHDLYEDDDYLHMVLDLCDAPDLHSRVTRRVYSEPEAASVMAQLM 120

Query: 121 HAVAHCHRHGVAHRDIKPDNILFDEWDSVKLADFGSTEMFKQGEESMSGVVGTPYYVAPE 180
            AVA CHR GVAHRDIKPDNILFDE + ++LADFGS E FK   E MSG+V TPYYVAPE
Sbjct: 121 QAVAQCHRLGVAHRDIKPDNILFDERNRLRLADFGSAETFKDRGEPMSGIVRTPYYVAPE 180

Query: 181 VLAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPS 240
           VLAG++Y EKVDVWSAGVVLY+MLAGFPPF+GESVVEIF AVLRANLRFPSRVFHSVSP+
Sbjct: 181 VLAGREYCEKVDVWSAGVVLYIMLAGFPPFYGESVVEIFEAVLRANLRFPSRVFHSVSPA 240

Query: 241 AKDLLRKMLCKDVSRRISAEQVL 263
           AKDLLR+MLCKDVSRR SAEQVL
Sbjct: 241 AKDLLRRMLCKDVSRRFSAEQVL 263

[Close]

BLAST of Cucsa.255380 vs. TrEMBL
Match: A0A067KZE1_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_02326 PE=3 SV=1)

HSP 1 Score: 377.9 bits (969), Expect = 1.4e-101
Identity = 190/263 (72.24%), Postives = 216/263 (82.13%), Query Frame = 1


Query: 1   MSDGFKRDYQVIEELGRGRFGTVFRCISKSCGGNYAVKIIDKRRISAGDSLDAECLVNET 60
           MS+   RDY + EE+GRGRFGTVFRCISKS G  +AVK IDK  +++GDSLDA+CL+ E 
Sbjct: 1   MSEALNRDYLISEEIGRGRFGTVFRCISKSTGETFAVKSIDKS-LTSGDSLDAQCLLTEP 60

Query: 61  KILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLQ-VFSEAEAARIMSQLM 120
           KILHLL PHPHI+ LHNLYEDE+HLHMV++LCSS DLH  I    V +EAEA  I  QLM
Sbjct: 61  KILHLLSPHPHIIQLHNLYEDETHLHMVIELCSSQDLHDLIIGNGVLTEAEARVIFIQLM 120

Query: 121 HAVAHCHRHGVAHRDIKPDNILFDEWDSVKLADFGSTEMFKQGEESMSGVVGTPYYVAPE 180
            AV+HCHR+GV HRDIK DNIL D   SVKLADFGS E+   GE +++GVVGTPYYVAPE
Sbjct: 121 EAVSHCHRYGVVHRDIKLDNILLDSRKSVKLADFGSAEVVMDGE-TIAGVVGTPYYVAPE 180

Query: 181 VLAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPS 240
           VLAG++YGEKVDVWSAGVVLY+MLAGFPPFHGE+ VEIF +VLR NLRFP R FHSVSP 
Sbjct: 181 VLAGREYGEKVDVWSAGVVLYIMLAGFPPFHGETAVEIFESVLRGNLRFPFRAFHSVSPV 240

Query: 241 AKDLLRKMLCKDVSRRISAEQVL 263
            KDLLRKMLCKDVS+R SAEQVL
Sbjct: 241 VKDLLRKMLCKDVSKRFSAEQVL 261

[Close]

BLAST of Cucsa.255380 vs. TrEMBL
Match: Q6ULS3_SOYBN (Phosphoenolpyruvate carboxylase kinase 4 OS=Glycine max GN=PpcK4 PE=2 SV=1)

HSP 1 Score: 369.8 bits (948), Expect = 3.7e-99
Identity = 192/263 (73.00%), Postives = 215/263 (81.75%), Query Frame = 1


Query: 1   MSDGFKRDYQVIEELGRGRFGTVFRCISKSCGGNYAVKIIDKRRISA-GDSLDAECLVNE 60
           MS   KRDY V EE+GRGRFGTVFRC S   G +YAVK IDK  I+A GDSLDA+CL+ E
Sbjct: 1   MSQDLKRDYVVSEEIGRGRFGTVFRCSSADSGHSYAVKSIDKVAITAAGDSLDAQCLLTE 60

Query: 61  TKILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLQVFSEAEAARIMSQLM 120
            KI+ LL PHPHI+ LH+LYEDE++LHMVLDLC  S  H R    V SE EAA +M QLM
Sbjct: 61  PKIVQLLSPHPHIVNLHDLYEDETNLHMVLDLCYESQFHHR----VMSEPEAASVMWQLM 120

Query: 121 HAVAHCHRHGVAHRDIKPDNILFDEWDSVKLADFGSTEMFKQGEESMSGVVGTPYYVAPE 180
            AVAHCHR GVAHRD+KPDNILFDE + +KLADFGS + FK+GE  MSGVVGTP+YVAPE
Sbjct: 121 QAVAHCHRLGVAHRDVKPDNILFDEENRLKLADFGSADTFKEGEP-MSGVVGTPHYVAPE 180

Query: 181 VLAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPS 240
           VLAG+DY EKVDVWSAGVVLY MLAGF PF G+S VEIF AVLRANLRFP+RVF SVSP+
Sbjct: 181 VLAGRDYNEKVDVWSAGVVLYQMLAGFLPFRGDSPVEIFEAVLRANLRFPTRVFCSVSPA 240

Query: 241 AKDLLRKMLCKDVSRRISAEQVL 263
           AKDLLR+MLCK+VSRR SAEQVL
Sbjct: 241 AKDLLRRMLCKEVSRRFSAEQVL 258

[Close]

BLAST of Cucsa.255380 vs. TrEMBL
Match: U5G1P1_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0010s08220g PE=3 SV=1)

HSP 1 Score: 369.0 bits (946), Expect = 6.3e-99
Identity = 181/263 (68.82%), Postives = 217/263 (82.51%), Query Frame = 1


Query: 1   MSDGFKRDYQVIEELGRGRFGTVFRCISKSCGGNYAVKIIDKRRISAGDSLDAECLVNET 60
           MS+   RDYQ+ EE+GRGRFGTVFRCIS+S G  +AVK IDK  +S GDSLD++CL+ E 
Sbjct: 1   MSEALDRDYQICEEIGRGRFGTVFRCISRSSGDYFAVKSIDKSLVS-GDSLDSQCLLTEP 60

Query: 61  KILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRI-TLQVFSEAEAARIMSQLM 120
           K LH L PHPHI+ LHNLYEDE+HLHMV+DLCS+ DLH  I T  V SE EA  + +QLM
Sbjct: 61  KTLHFLSPHPHIIQLHNLYEDETHLHMVIDLCSNQDLHDLIITNGVLSENEARVVFTQLM 120

Query: 121 HAVAHCHRHGVAHRDIKPDNILFDEWDSVKLADFGSTEMFKQGEESMSGVVGTPYYVAPE 180
            A++HCH +G+ HRDIKPDNILFDE +++K+ADFGS E+  +GE  +SGVVGTPYYVAPE
Sbjct: 121 KAISHCHTYGIVHRDIKPDNILFDEENTIKVADFGSAEVVMEGE-MVSGVVGTPYYVAPE 180

Query: 181 VLAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPS 240
           VL G++YG+KVDVWSAGVVLY+MLAGFPPF+GE+  +IF AVLR NLRFP+RVF SVS S
Sbjct: 181 VLLGREYGKKVDVWSAGVVLYIMLAGFPPFYGETADKIFEAVLRGNLRFPARVFQSVSAS 240

Query: 241 AKDLLRKMLCKDVSRRISAEQVL 263
            KDLLR++LCKDVSRR SAEQVL
Sbjct: 241 VKDLLRRILCKDVSRRFSAEQVL 261

[Close]

BLAST of Cucsa.255380 vs. TAIR10
Match: AT1G08650.1 (AT1G08650.1 phosphoenolpyruvate carboxylase kinase 1)

HSP 1 Score: 268.5 bits (685), Expect = 5.9e-72
Identity = 141/257 (54.86%), Postives = 179/257 (69.65%), Query Frame = 1


Query: 9   YQVIEELGRGRFGTVFRCISKSCGGNYAVKIIDKRRISAGDSLDAECLVNETKILHLLYP 68
           YQ+ EE+GRGRFGTV R  + + G  +A K IDK  +S  D LD  CL NE K++ LL  
Sbjct: 15  YQICEEIGRGRFGTVSRVYAPATGDFFACKTIDKASLS--DDLDRACLDNEPKLMALLSY 74

Query: 69  HPHILALHNLYEDESHLHMVLDLC--SSSDLHRRITLQVFSEAEAARIMSQLMHAVAHCH 128
           HP+I+ +H+L + +S L + ++L   S S   R ++   F E + A    Q++ A++HCH
Sbjct: 75  HPNIVQIHDLIDTDSTLSIFMELVHPSVSIYDRLVSSGTFFEPQTASFAKQILQALSHCH 134

Query: 129 RHGVAHRDIKPDNILFD-EWDSVKLADFGSTEMFKQGEESMSGVVGTPYYVAPEVLAGKD 188
           R+GV HRDIKP+NIL D   D+VK+ DFGS     +GE +  GVVGTPYYVAPEVL G  
Sbjct: 135 RYGVVHRDIKPENILVDLRNDTVKICDFGSGIWLGEGETT-EGVVGTPYYVAPEVLMGYS 194

Query: 189 YGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPSAKDLLR 248
           YGEKVD+WSAGVVLY MLAG PPF+GE+  EIF AVLR NLRFP+++F  VS  AKD LR
Sbjct: 195 YGEKVDLWSAGVVLYTMLAGTPPFYGETAEEIFEAVLRGNLRFPTKIFRGVSSMAKDFLR 254

Query: 249 KMLCKDVSRRISAEQVL 263
           K++CKD SRR SAEQ L
Sbjct: 255 KLICKDASRRFSAEQAL 268

[Close]

BLAST of Cucsa.255380 vs. TAIR10
Match: AT3G04530.1 (AT3G04530.1 phosphoenolpyruvate carboxylase kinase 2)

HSP 1 Score: 257.7 bits (657), Expect = 1.0e-68
Identity = 136/261 (52.11%), Postives = 179/261 (68.58%), Query Frame = 1


Query: 6   KRDYQVIEELGRGRFGTVFRCISKSCGGNYAVKIIDKRRISAGDSLDAECLVNETKILHL 65
           + +YQ+ +E+GRGRFGT+ RC S +    YA K IDKR +   D+LD EC+  E +I+ +
Sbjct: 8   ENNYQLCDEIGRGRFGTITRCFSPATKEFYACKTIDKRVLI--DALDRECIETEPRIMAM 67

Query: 66  LYPHPHILALHNLYEDESHLHMVLDLCSSS-DLHRRITLQV--FSEAEAARIMSQLMHAV 125
           L PHP+I+ + +LYE E  L +V++L      ++ R+       SE+E+A    Q++ A+
Sbjct: 68  LPPHPNIIRIFDLYETEDSLAIVMELVDPPMTIYDRLISAGGRLSESESASYAKQILSAL 127

Query: 126 AHCHRHGVAHRDIKPDNILFDEWDS-VKLADFGSTEMFKQGEESMSGVVGTPYYVAPEVL 185
           AHCHR  V HRD+KPDN+L D     VKL DFGS      G E+  GVVGTPYYVAPEV+
Sbjct: 128 AHCHRCDVVHRDVKPDNVLVDLVSGGVKLCDFGSAVWL--GGETAEGVVGTPYYVAPEVV 187

Query: 186 AGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPSAK 245
            G+ Y EKVD+WSAGVV+Y MLAG PPF+GE+  +IF ++LR NLRFP + F SVS  AK
Sbjct: 188 MGRKYDEKVDIWSAGVVIYTMLAGEPPFNGETAEDIFESILRGNLRFPPKKFGSVSSEAK 247

Query: 246 DLLRKMLCKDVSRRISAEQVL 263
           DLLRKM+C+DVSRR SAE  L
Sbjct: 248 DLLRKMICRDVSRRFSAEDAL 264

[Close]

BLAST of Cucsa.255380 vs. TAIR10
Match: AT3G10660.1 (AT3G10660.1 calmodulin-domain protein kinase cdpk isoform 2)

HSP 1 Score: 211.5 bits (537), Expect = 8.6e-55
Identity = 115/265 (43.40%), Postives = 168/265 (63.40%), Query Frame = 1


Query: 2   SDGFKRDYQVIEELGRGRFGTVFRCISKSCGGNYAVKIIDKRRISAGDSLDAECLVNETK 61
           ++ FK  Y +  +LG+G+FGT F C+ K  G  YA K I KR++   +  D E +  E +
Sbjct: 179 TENFKEFYSLGRKLGQGQFGTTFLCLEKGTGNEYACKSISKRKLLTDE--DVEDVRREIQ 238

Query: 62  ILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLQV-FSEAEAARIMSQLMH 121
           I+H L  HP+++++   YED   +H+V++LCS  +L  RI  +  ++E +AA +   ++ 
Sbjct: 239 IMHHLAGHPNVISIKGAYEDVVAVHLVMELCSGGELFDRIIQRGHYTERKAAELARTIVG 298

Query: 122 AVAHCHRHGVAHRDIKPDNILF---DEWDSVKLADFGSTEMFKQGEESMSGVVGTPYYVA 181
            +  CH  GV HRD+KP+N LF   +E   +K  DFG +  FK  +E  + VVG+PYYVA
Sbjct: 299 VLEACHSLGVMHRDLKPENFLFVSREEDSLLKTIDFGLSMFFKP-DEVFTDVVGSPYYVA 358

Query: 182 PEVLAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVS 241
           PEVL  K YG + DVWSAGV++Y++L+G PPF  E+   IF  VL  +L F S  + S+S
Sbjct: 359 PEVLR-KRYGPESDVWSAGVIVYILLSGVPPFWAETEQGIFEQVLHGDLDFSSDPWPSIS 418

Query: 242 PSAKDLLRKMLCKDVSRRISAEQVL 263
            SAKDL+RKML +D  RR++A QVL
Sbjct: 419 ESAKDLVRKMLVRDPKRRLTAHQVL 439

[Close]

BLAST of Cucsa.255380 vs. TAIR10
Match: AT5G04870.1 (AT5G04870.1 calcium dependent protein kinase 1)

HSP 1 Score: 204.5 bits (519), Expect = 1.0e-52
Identity = 111/265 (41.89%), Postives = 167/265 (63.02%), Query Frame = 1


Query: 2   SDGFKRDYQVIEELGRGRFGTVFRCISKSCGGNYAVKIIDKRRISAGDSLDAECLVNETK 61
           ++ FK  Y +  +LG+G+FGT F C+ K+ G  +A K I KR++   +  D E +  E +
Sbjct: 143 TENFKEFYSLGRKLGQGQFGTTFLCVEKTTGKEFACKSIAKRKLLTDE--DVEDVRREIQ 202

Query: 62  ILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLQV-FSEAEAARIMSQLMH 121
           I+H L  HP+++++   YED   +H+V++ C+  +L  RI  +  ++E +AA +   ++ 
Sbjct: 203 IMHHLAGHPNVISIKGAYEDVVAVHLVMECCAGGELFDRIIQRGHYTERKAAELTRTIVG 262

Query: 122 AVAHCHRHGVAHRDIKPDNILF---DEWDSVKLADFGSTEMFKQGEESMSGVVGTPYYVA 181
            V  CH  GV HRD+KP+N LF    E   +K  DFG +  FK  ++  + VVG+PYYVA
Sbjct: 263 VVEACHSLGVMHRDLKPENFLFVSKHEDSLLKTIDFGLSMFFKP-DDVFTDVVGSPYYVA 322

Query: 182 PEVLAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVS 241
           PEVL  K YG + DVWSAGV++Y++L+G PPF  E+   IF  VL  +L F S  + S+S
Sbjct: 323 PEVLR-KRYGPEADVWSAGVIVYILLSGVPPFWAETEQGIFEQVLHGDLDFSSDPWPSIS 382

Query: 242 PSAKDLLRKMLCKDVSRRISAEQVL 263
            SAKDL+RKML +D  +R++A QVL
Sbjct: 383 ESAKDLVRKMLVRDPKKRLTAHQVL 403

[Close]

BLAST of Cucsa.255380 vs. TAIR10
Match: AT2G38910.1 (AT2G38910.1 calcium-dependent protein kinase 20)

HSP 1 Score: 204.1 bits (518), Expect = 1.4e-52
Identity = 110/265 (41.51%), Postives = 168/265 (63.40%), Query Frame = 1


Query: 2   SDGFKRDYQVIEELGRGRFGTVFRCISKSCGGNYAVKIIDKRRISAGDSLDAECLVNETK 61
           ++  K  Y V  +LG+G+FGT F C+ K  G  +A K I KR+++  +  D E +  E +
Sbjct: 127 TENLKDIYSVGRKLGQGQFGTTFLCVDKKTGKEFACKTIAKRKLTTPE--DVEDVRREIQ 186

Query: 62  ILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLQV-FSEAEAARIMSQLMH 121
           I+H L  HP+++ +   YED   +H+V+++C+  +L  RI  +  ++E +AA +   ++ 
Sbjct: 187 IMHHLSGHPNVIQIVGAYEDAVAVHVVMEICAGGELFDRIIQRGHYTEKKAAELARIIVG 246

Query: 122 AVAHCHRHGVAHRDIKPDNILF---DEWDSVKLADFGSTEMFKQGEESMSGVVGTPYYVA 181
            +  CH  GV HRD+KP+N LF   DE  ++K  DFG +  FK GE + + VVG+PYYVA
Sbjct: 247 VIEACHSLGVMHRDLKPENFLFVSGDEEAALKTIDFGLSVFFKPGE-TFTDVVGSPYYVA 306

Query: 182 PEVLAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVS 241
           PEVL  K Y  + DVWSAGV++Y++L+G PPF  E+   IF  VL+ +L F S  + SVS
Sbjct: 307 PEVLR-KHYSHECDVWSAGVIIYILLSGVPPFWDETEQGIFEQVLKGDLDFISEPWPSVS 366

Query: 242 PSAKDLLRKMLCKDVSRRISAEQVL 263
            SAKDL+R+ML +D  +R++  +VL
Sbjct: 367 ESAKDLVRRMLIRDPKKRMTTHEVL 387

[Close]

BLAST of Cucsa.255380 vs. NCBI nr
Match: gi|449432560|ref|XP_004134067.1| (PREDICTED: phosphoenolpyruvate carboxylase kinase 1-like [Cucumis sativus])

HSP 1 Score: 532.3 bits (1370), Expect = 6.2e-148
Identity = 262/262 (100.00%), Postives = 262/262 (100.00%), Query Frame = 1


Query: 1   MSDGFKRDYQVIEELGRGRFGTVFRCISKSCGGNYAVKIIDKRRISAGDSLDAECLVNET 60
           MSDGFKRDYQVIEELGRGRFGTVFRCISKSCGGNYAVKIIDKRRISAGDSLDAECLVNET
Sbjct: 1   MSDGFKRDYQVIEELGRGRFGTVFRCISKSCGGNYAVKIIDKRRISAGDSLDAECLVNET 60

Query: 61  KILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLQVFSEAEAARIMSQLMH 120
           KILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLQVFSEAEAARIMSQLMH
Sbjct: 61  KILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLQVFSEAEAARIMSQLMH 120

Query: 121 AVAHCHRHGVAHRDIKPDNILFDEWDSVKLADFGSTEMFKQGEESMSGVVGTPYYVAPEV 180
           AVAHCHRHGVAHRDIKPDNILFDEWDSVKLADFGSTEMFKQGEESMSGVVGTPYYVAPEV
Sbjct: 121 AVAHCHRHGVAHRDIKPDNILFDEWDSVKLADFGSTEMFKQGEESMSGVVGTPYYVAPEV 180

Query: 181 LAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPSA 240
           LAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPSA
Sbjct: 181 LAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPSA 240

Query: 241 KDLLRKMLCKDVSRRISAEQVL 263
           KDLLRKMLCKDVSRRISAEQVL
Sbjct: 241 KDLLRKMLCKDVSRRISAEQVL 262

[Close]

BLAST of Cucsa.255380 vs. NCBI nr
Match: gi|659076149|ref|XP_008438527.1| (PREDICTED: phosphoenolpyruvate carboxylase kinase 1-like [Cucumis melo])

HSP 1 Score: 521.5 bits (1342), Expect = 1.1e-144
Identity = 256/262 (97.71%), Postives = 259/262 (98.85%), Query Frame = 1


Query: 1   MSDGFKRDYQVIEELGRGRFGTVFRCISKSCGGNYAVKIIDKRRISAGDSLDAECLVNET 60
           MSDGFKRDYQVIEELGRGRFG VFRCIS+S GGNYAVKIIDKRRISAGDSLDAECLVNET
Sbjct: 1   MSDGFKRDYQVIEELGRGRFGIVFRCISRSSGGNYAVKIIDKRRISAGDSLDAECLVNET 60

Query: 61  KILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLQVFSEAEAARIMSQLMH 120
           KILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLQ+FSEAEAARIMSQLMH
Sbjct: 61  KILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLQIFSEAEAARIMSQLMH 120

Query: 121 AVAHCHRHGVAHRDIKPDNILFDEWDSVKLADFGSTEMFKQGEESMSGVVGTPYYVAPEV 180
           AVAHCHRHGVAHRDIKPDNILFDEWDSVKLADFGS EMFKQGEESM+GVVGTPYYVAPEV
Sbjct: 121 AVAHCHRHGVAHRDIKPDNILFDEWDSVKLADFGSAEMFKQGEESMTGVVGTPYYVAPEV 180

Query: 181 LAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPSA 240
           LAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPSA
Sbjct: 181 LAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPSA 240

Query: 241 KDLLRKMLCKDVSRRISAEQVL 263
           KDLLRKMLCKDVSRRISAEQVL
Sbjct: 241 KDLLRKMLCKDVSRRISAEQVL 262

[Close]

BLAST of Cucsa.255380 vs. NCBI nr
Match: gi|1009140810|ref|XP_015887853.1| (PREDICTED: phosphoenolpyruvate carboxylase kinase 1-like [Ziziphus jujuba])

HSP 1 Score: 394.0 bits (1011), Expect = 2.6e-106
Identity = 196/263 (74.52%), Postives = 224/263 (85.17%), Query Frame = 1


Query: 1   MSDGFKRDYQVIEELGRGRFGTVFRCISKSCGG-NYAVKIIDKRRISAGDSLDAECLVNE 60
           MS+  KRDY + +E+GRGRFGTVFRC     GG + AVK IDK R+++GD L+++CL  E
Sbjct: 1   MSESLKRDYIICDEIGRGRFGTVFRCFPAIGGGYSLAVKSIDK-RLTSGDFLESQCLFTE 60

Query: 61  TKILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLQVFSEAEAARIMSQLM 120
            KIL LL PHPHI+ +H LYEDE+HLH+VLDLC + DLH RITL+VFSE EAA +MSQLM
Sbjct: 61  PKILQLLSPHPHIINMHGLYEDETHLHLVLDLCDTPDLHCRITLRVFSEPEAAVVMSQLM 120

Query: 121 HAVAHCHRHGVAHRDIKPDNILFDEWDSVKLADFGSTEMFKQGEESMSGVVGTPYYVAPE 180
            AVAHCHR GVAHRDIKPDNILFDE   +KLADFGS E+FK+G E MSG+VGTPYYVAPE
Sbjct: 121 EAVAHCHRLGVAHRDIKPDNILFDERSRLKLADFGSAEIFKEG-EPMSGIVGTPYYVAPE 180

Query: 181 VLAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPS 240
           VL+GKDYGEKVDVWSAGVVLY+MLAGFPPF+GES VEIF AVLR NLRFP+R FHSVSP+
Sbjct: 181 VLSGKDYGEKVDVWSAGVVLYIMLAGFPPFYGESAVEIFEAVLRGNLRFPTRAFHSVSPT 240

Query: 241 AKDLLRKMLCKDVSRRISAEQVL 263
           AKDLLR+MLCKDVSRR SAEQVL
Sbjct: 241 AKDLLRRMLCKDVSRRFSAEQVL 261

[Close]

BLAST of Cucsa.255380 vs. NCBI nr
Match: gi|703130843|ref|XP_010104734.1| (Phosphoenolpyruvate carboxylase kinase 1 [Morus notabilis])

HSP 1 Score: 392.1 bits (1006), Expect = 1.0e-105
Identity = 194/263 (73.76%), Postives = 222/263 (84.41%), Query Frame = 1


Query: 1   MSDGFKRDYQVIEELGRGRFGTVFRCISKSCGGNY-AVKIIDKRRISAGDSLDAECLVNE 60
           MS    RDYQV+EE+GRGRFG V+RC   +  G++ AVK IDKR ISAGDSLDA+CL +E
Sbjct: 1   MSQALNRDYQVLEEIGRGRFGVVYRCSPVTNSGDFFAVKSIDKRLISAGDSLDAQCLFSE 60

Query: 61  TKILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLQVFSEAEAARIMSQLM 120
            KIL  L PHPHI+ LH+LYED+ +LHMVLDLC + DLH R+T +V+SE EAA +M+QLM
Sbjct: 61  PKILRFLSPHPHIINLHDLYEDDDYLHMVLDLCDAPDLHSRVTRRVYSEPEAASVMAQLM 120

Query: 121 HAVAHCHRHGVAHRDIKPDNILFDEWDSVKLADFGSTEMFKQGEESMSGVVGTPYYVAPE 180
            AVA CHR GVAHRDIKPDNILFDE + ++LADFGS E FK   E MSG+V TPYYVAPE
Sbjct: 121 QAVAQCHRLGVAHRDIKPDNILFDERNRLRLADFGSAETFKDRGEPMSGIVRTPYYVAPE 180

Query: 181 VLAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPS 240
           VLAG++Y EKVDVWSAGVVLY+MLAGFPPF+GESVVEIF AVLRANLRFPSRVFHSVSP+
Sbjct: 181 VLAGREYCEKVDVWSAGVVLYIMLAGFPPFYGESVVEIFEAVLRANLRFPSRVFHSVSPA 240

Query: 241 AKDLLRKMLCKDVSRRISAEQVL 263
           AKDLLR+MLCKDVSRR SAEQVL
Sbjct: 241 AKDLLRRMLCKDVSRRFSAEQVL 263

[Close]

BLAST of Cucsa.255380 vs. NCBI nr
Match: gi|802581642|ref|XP_012069828.1| (PREDICTED: phosphoenolpyruvate carboxylase kinase 1-like [Jatropha curcas])

HSP 1 Score: 377.9 bits (969), Expect = 2.0e-101
Identity = 190/263 (72.24%), Postives = 216/263 (82.13%), Query Frame = 1


Query: 1   MSDGFKRDYQVIEELGRGRFGTVFRCISKSCGGNYAVKIIDKRRISAGDSLDAECLVNET 60
           MS+   RDY + EE+GRGRFGTVFRCISKS G  +AVK IDK  +++GDSLDA+CL+ E 
Sbjct: 1   MSEALNRDYLISEEIGRGRFGTVFRCISKSTGETFAVKSIDKS-LTSGDSLDAQCLLTEP 60

Query: 61  KILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLQ-VFSEAEAARIMSQLM 120
           KILHLL PHPHI+ LHNLYEDE+HLHMV++LCSS DLH  I    V +EAEA  I  QLM
Sbjct: 61  KILHLLSPHPHIIQLHNLYEDETHLHMVIELCSSQDLHDLIIGNGVLTEAEARVIFIQLM 120

Query: 121 HAVAHCHRHGVAHRDIKPDNILFDEWDSVKLADFGSTEMFKQGEESMSGVVGTPYYVAPE 180
            AV+HCHR+GV HRDIK DNIL D   SVKLADFGS E+   GE +++GVVGTPYYVAPE
Sbjct: 121 EAVSHCHRYGVVHRDIKLDNILLDSRKSVKLADFGSAEVVMDGE-TIAGVVGTPYYVAPE 180

Query: 181 VLAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPS 240
           VLAG++YGEKVDVWSAGVVLY+MLAGFPPFHGE+ VEIF +VLR NLRFP R FHSVSP 
Sbjct: 181 VLAGREYGEKVDVWSAGVVLYIMLAGFPPFHGETAVEIFESVLRGNLRFPFRAFHSVSPV 240

Query: 241 AKDLLRKMLCKDVSRRISAEQVL 263
            KDLLRKMLCKDVS+R SAEQVL
Sbjct: 241 VKDLLRKMLCKDVSKRFSAEQVL 261

The following BLAST results are available for this feature:

BLAST of Cucsa.255380 vs. Swiss-Prot


Match Name	E-value	Identity	Description
PPCK1_ARATH	1.0e-70	54.86	Phosphoenolpyruvate carboxylase kinase 1 OS=Arabidopsis thaliana GN=PPCK1 PE=1 S...	[more]
PPCK2_ARATH	1.8e-67	52.11	Phosphoenolpyruvate carboxylase kinase 2 OS=Arabidopsis thaliana GN=PPCK2 PE=1 S...	[more]
CDPK2_ARATH	1.5e-53	43.40	Calcium-dependent protein kinase 2 OS=Arabidopsis thaliana GN=CPK2 PE=1 SV=1	[more]
CDPK_SOYBN	4.4e-53	44.19	Calcium-dependent protein kinase SK5 OS=Glycine max PE=1 SV=1	[more]
CDPK1_ARATH	1.9e-51	41.89	Calcium-dependent protein kinase 1 OS=Arabidopsis thaliana GN=CPK1 PE=1 SV=1	[more]

BLAST of Cucsa.255380 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L743_CUCSA	4.3e-148	100.00	Phosphoenolpyruvate carboxylase kinase 4 OS=Cucumis sativus GN=Csa_3G135070 PE=3...	[more]
W9RPC3_9ROSA	7.0e-106	73.76	Phosphoenolpyruvate carboxylase kinase 1 OS=Morus notabilis GN=L484_021421 PE=3 ...	[more]
A0A067KZE1_JATCU	1.4e-101	72.24	Uncharacterized protein OS=Jatropha curcas GN=JCGZ_02326 PE=3 SV=1	[more]
Q6ULS3_SOYBN	3.7e-99	73.00	Phosphoenolpyruvate carboxylase kinase 4 OS=Glycine max GN=PpcK4 PE=2 SV=1	[more]
U5G1P1_POPTR	6.3e-99	68.82	Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0010s08220g PE=3 SV=1	[more]

BLAST of Cucsa.255380 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G08650.1	5.9e-72	54.86	phosphoenolpyruvate carboxylase kinase 1	[more]
AT3G04530.1	1.0e-68	52.11	phosphoenolpyruvate carboxylase kinase 2	[more]
AT3G10660.1	8.6e-55	43.40	calmodulin-domain protein kinase cdpk isoform 2	[more]
AT5G04870.1	1.0e-52	41.89	calcium dependent protein kinase 1	[more]
AT2G38910.1	1.4e-52	41.51	calcium-dependent protein kinase 20	[more]

BLAST of Cucsa.255380 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449432560\|ref\|XP_004134067.1\|	6.2e-148	100.00	PREDICTED: phosphoenolpyruvate carboxylase kinase 1-like [Cucumis sativus]	[more]
gi\|659076149\|ref\|XP_008438527.1\|	1.1e-144	97.71	PREDICTED: phosphoenolpyruvate carboxylase kinase 1-like [Cucumis melo]	[more]
gi\|1009140810\|ref\|XP_015887853.1\|	2.6e-106	74.52	PREDICTED: phosphoenolpyruvate carboxylase kinase 1-like [Ziziphus jujuba]	[more]
gi\|703130843\|ref\|XP_010104734.1\|	1.0e-105	73.76	Phosphoenolpyruvate carboxylase kinase 1 [Morus notabilis]	[more]
gi\|802581642\|ref\|XP_012069828.1\|	2.0e-101	72.24	PREDICTED: phosphoenolpyruvate carboxylase kinase 1-like [Jatropha curcas]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR000719	Prot_kinase_dom
IPR008271	Ser/Thr_kinase_AS
IPR011009	Kinase-like_dom_sf
IPR017441	Protein_kinase_ATP_BS
IPR000719	Prot_kinase_dom
IPR008271	Ser/Thr_kinase_AS
IPR011009	Kinase-like_dom_sf
IPR017441	Protein_kinase_ATP_BS

Vocabulary: **Molecular Function**
Term	Definition
GO:0004672	protein kinase activity
GO:0005524	ATP binding
GO:0004672	protein kinase activity
GO:0005524	ATP binding

Vocabulary: **Biological Process**
Term	Definition
GO:0006468	protein phosphorylation
GO:0006468	protein phosphorylation

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004672	protein kinase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsa.255380.2	Cucsa.255380.2	mRNA
Cucsa.255380.1	Cucsa.255380.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000719	Protein kinase domain	PFAM	PF00069	Pkinase	coord: 9..267 score: 2.4
IPR000719	Protein kinase domain	SMART	SM00220	serkin_6	coord: 9..267 score: 1.3
IPR000719	Protein kinase domain	PROFILE	PS50011	PROTEIN_KINASE_DOM	coord: 9..267 score: 50
IPR008271	Serine/threonine-protein kinase, active site	PROSITE	PS00108	PROTEIN_KINASE_ST	coord: 130..142 scor
IPR011009	Protein kinase-like domain	unknown	SSF56112	Protein kinase-like (PK-like)	coord: 7..272 score: 1.75
IPR017441	Protein kinase, ATP binding site	PROSITE	PS00107	PROTEIN_KINASE_ATP	coord: 15..38 scor
None	No IPR available	GENE3D	G3DSA:1.10.510.10		coord: 120..273 score: 3.5
None	No IPR available	GENE3D	G3DSA:3.30.200.20		coord: 6..119 score: 4.1
None	No IPR available	PIR	PIRSF000654	ILK	coord: 4..265 score: 4.1
None	No IPR available	PANTHER	PTHR24349	SERINE/THREONINE-PROTEIN KINASE	coord: 1..268 score: 1.2E
None	No IPR available	PANTHER	PTHR24349:SF122	SUBFAMILY NOT NAMED	coord: 1..268 score: 1.2E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cucsa.255380	Cla021521	Watermelon (97103) v1	cgywmB479
Cucsa.255380	Csa3G135070	Cucumber (Chinese Long) v2	cgycuB366
Cucsa.255380	MELO3C006426	Melon (DHL92) v3.5.1	cgymeB439
Cucsa.255380	ClCG05G004340	Watermelon (Charleston Gray)	cgywcgB451
Cucsa.255380	CSPI03G11970	Wild cucumber (PI 183967)	cgycpiB383
Cucsa.255380	CmaCh14G018120	Cucurbita maxima (Rimu)	cgycmaB0709
Cucsa.255380	CmaCh06G013270	Cucurbita maxima (Rimu)	cgycmaB0727
Cucsa.255380	CmoCh14G018500	Cucurbita moschata (Rifu)	cgycmoB0700
Cucsa.255380	CmoCh06G013310	Cucurbita moschata (Rifu)	cgycmoB0718
Cucsa.255380	Lsi05G017290	Bottle gourd (USVL1VR-Ls)	cgylsiB415
Cucsa.255380	Cp4.1LG03g09920	Cucurbita pepo (Zucchini)	cgycpeB0682
Cucsa.255380	Cp4.1LG08g03440	Cucurbita pepo (Zucchini)	cgycpeB0687
Cucsa.255380	MELO3C006426.2	Melon (DHL92) v3.6.1	cgymedB432
Cucsa.255380	CsaV3_3G012120	Cucumber (Chinese Long) v3	cgycucB387
Cucsa.255380	Cla97C05G085110	Watermelon (97103) v2	cgywmbB452
Cucsa.255380	Bhi01G000527	Wax gourd	cgywgoB525
Cucsa.255380	Carg16562	Silver-seed gourd	carcgyB0276
Cucsa.255380	Carg06217	Silver-seed gourd	carcgyB1077

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cucsa.255380	Silver-seed gourd	carcgyB0269
Cucsa.255380	Silver-seed gourd	carcgyB0847
Cucsa.255380	Silver-seed gourd	carcgyB0887
Cucsa.255380	Silver-seed gourd	carcgyB1190
Cucsa.255380	Cucumber (Chinese Long) v3	cgycucB384
Cucsa.255380	Cucumber (Chinese Long) v3	cgycucB389
Cucsa.255380	Watermelon (97103) v2	cgywmbB453
Cucsa.255380	Watermelon (97103) v2	cgywmbB456
Cucsa.255380	Wax gourd	cgywgoB510
Cucsa.255380	Wax gourd	cgywgoB528
Cucsa.255380	Cucumber (Gy14) v1	cgycgyB133
Cucsa.255380	Cucurbita maxima (Rimu)	cgycmaB0715
Cucsa.255380	Cucurbita maxima (Rimu)	cgycmaB0717
Cucsa.255380	Cucurbita maxima (Rimu)	cgycmaB0719
Cucsa.255380	Cucurbita maxima (Rimu)	cgycmaB0732
Cucsa.255380	Cucurbita moschata (Rifu)	cgycmoB0708
Cucsa.255380	Cucurbita moschata (Rifu)	cgycmoB0710
Cucsa.255380	Cucurbita moschata (Rifu)	cgycmoB0713
Cucsa.255380	Cucurbita moschata (Rifu)	cgycmoB0721
Cucsa.255380	Wild cucumber (PI 183967)	cgycpiB378
Cucsa.255380	Cucumber (Chinese Long) v2	cgycuB362
Cucsa.255380	Melon (DHL92) v3.5.1	cgymeB433
Cucsa.255380	Melon (DHL92) v3.5.1	cgymeB434
Cucsa.255380	Watermelon (Charleston Gray)	cgywcgB452
Cucsa.255380	Watermelon (Charleston Gray)	cgywcgB457
Cucsa.255380	Watermelon (97103) v1	cgywmB475
Cucsa.255380	Watermelon (97103) v1	cgywmB483
Cucsa.255380	Cucurbita pepo (Zucchini)	cgycpeB0670
Cucsa.255380	Cucurbita pepo (Zucchini)	cgycpeB0675
Cucsa.255380	Cucurbita pepo (Zucchini)	cgycpeB0683
Cucsa.255380	Bottle gourd (USVL1VR-Ls)	cgylsiB412
Cucsa.255380	Bottle gourd (USVL1VR-Ls)	cgylsiB417
Cucsa.255380	Melon (DHL92) v3.6.1	cgymedB428
Cucsa.255380	Melon (DHL92) v3.6.1	cgymedB429