Cucsa.255090 (gene) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.255090
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	Protease inhibitor protein
Location	scaffold02229 : 3195267 .. 3196252 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ACCCAAATCAGAATTAAAAGGACAGCAAGAGTCTTCCAAATCCATACATATAATATACCCAACCTTCAACAATGGCTGAAGAATCTGCTAGAGGTAAGTCCATTATATATATTCACCCTATCAAATTGCTTTTTAATTTTTCAAAAATACTTGTTCCTAAGTAGTTTTAATTTCAAAACAATCTTGTTTTTGAGAAATTAGTTTAGATTTCGAATAAATATATGGTTTGTAAACTAAATGAAATTTTATCAACTATACAAGTATTGAAAGTCAAATGATATCAATTCAACACATAAAAACAATACCCCGATGCCTGTTTATGCAATTTGAGGTATATTCTACCAAAGGTTATACTTTGGTTGGATTTTAGATTATATCTATTTGCAAAATTTAACGTATAATCTATATATAATTCTTAAATAGAAAAAACTCAATGGCCAGAGCTTGTTGGCGTAGACTATTCTACTGCTGCTACGACAATAGAAACAGAGAATTCTCATGTGAAGGCTATCAAGTTTCTAGTTGGTAGTCCCATGTATCTGAATTTTGATCCTCGCCGAGTTCGGGTTGTTTGCGATACACAAGACAAGGTTGTTGAAATGCCCTACGTCGGTTAAAGATTGGGTGATGTGTATAAGGTGAATCATATGTATAATGTCTATGTCATTATGTCATTCCAATATTGTACTAATAAGCGGTATTTCTACTGTTTCAACTCTTGTTTCAAGTGTGTTTATTTGTAATAAATTGTTGAATAAATTGTTCCAATAAACATCAACAAGGACCTATCTTTAGATATTGCTCATGCACGTATTATATTCTTAATGTAAATTGTGGGCATTTCATCTAATCTATTGGTACGCCCACAAATAGAATTTAAATCTTAAAGCTTATCCCTTCCATTTCTTTCTTCAATTCATGGGAGAGAAAAAGTTCACTTCAAAGATGGTTTTCCAATGGCTTTACCAACTTCGTCACCGCCACAA

mRNA sequence

ACCCAAATCAGAATTAAAAGGACAGCAAGAGTCTTCCAAATCCATACATATAATATACCCAACCTTCAACAATGGCTGAAGAATCTGCTAGAGAGCTTGTTGGCGTAGACTATTCTACTGCTGCTACGACAATAGAAACAGAGAATTCTCATGTGAAGGCTATCAAGTTTCTAGTTGGTAGTCCCATGTATCTGAATTTTGATCCTCGCCGAGTTCGGGTTGTTTGCGATACACAAGACAAGGTTGTTGAAATGCCCTACGTCGGTTAAAGATTGGGTGATGTGTATAAGGTGAATCATATGTATAATGTCTATGTCATTATGTCATTCCAATATTGTACTAATAAGCGGTATTTCTACTGTTTCAACTCTTGTTTCAAGTGTGTTTATTTGTAATAAATTGTTGAATAAATTGTTCCAATAAACATCAACAAGGACCTATCTTTAGATATTGCTCATGCACGTATTATATTCTTAATGTAAATTGTGGGCATTTCATCTAATCTATTGGTACGCCCACAAATAGAATTTAAATCTTAAAGCTTATCCCTTCCATTTCTTTCTTCAATTCATGGGAGAGAAAAAGTTCACTTCAAAGATGGTTTTCCAATGGCTTTACCAACTTCGTCACCGCCACAA

Coding sequence (CDS)

ATGGCTGAAGAATCTGCTAGAGAGCTTGTTGGCGTAGACTATTCTACTGCTGCTACGACAATAGAAACAGAGAATTCTCATGTGAAGGCTATCAAGTTTCTAGTTGGTAGTCCCATGTATCTGAATTTTGATCCTCGCCGAGTTCGGGTTGTTTGCGATACACAAGACAAGGTTGTTGAAATGCCCTACGTCGGTTAA

Protein sequence

MAEESARELVGVDYSTAATTIETENSHVKAIKFLVGSPMYLNFDPRRVRVVCDTQDKVVEMPYVG*

Homology

[Close]

BLAST of Cucsa.255090 vs. Swiss-Prot
Match: HPI_HEVBR (Protease inhibitor HPI OS=Hevea brasiliensis GN=PI1 PE=1 SV=2)

HSP 1 Score: 51.6 bits (122), Expect = 4.2e-06
Identity = 27/63 (42.86%), Postives = 37/63 (58.73%), Query Frame = 1


Query: 9  KTQWPELVGVDYSTAATTIETENSHVKAIKFLVGSPMYLNFDPRRVRVVCDTQDKVVEMP 68
          K  WPELVG +   AA  I+TEN++VKAI    G P+  + +  RVRV  D    V ++P
Sbjct: 8  KNSWPELVGTNGDIAAGIIQTENANVKAIVVKEGLPITQDLNFNRVRVFVDENRVVTQVP 67

Query: 69 YVG 72
           +G
Sbjct: 68 AIG 70

[Close]

BLAST of Cucsa.255090 vs. TrEMBL
Match: A0A0A0L4I0_CUCSA (Protease inhibitor protein OS=Cucumis sativus GN=Csa_3G142400 PE=4 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 1.1e-32
Identity = 71/71 (100.00%), Postives = 71/71 (100.00%), Query Frame = 1


Query: 1  MAEESAREKTQWPELVGVDYSTAATTIETENSHVKAIKFLVGSPMYLNFDPRRVRVVCDT 60
          MAEESAREKTQWPELVGVDYSTAATTIETENSHVKAIKFLVGSPMYLNFDPRRVRVVCDT
Sbjct: 1  MAEESAREKTQWPELVGVDYSTAATTIETENSHVKAIKFLVGSPMYLNFDPRRVRVVCDT 60

Query: 61 QDKVVEMPYVG 72
          QDKVVEMPYVG
Sbjct: 61 QDKVVEMPYVG 71

[Close]

BLAST of Cucsa.255090 vs. TrEMBL
Match: A0A0A0L9Z4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G142410 PE=4 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 1.8e-16
Identity = 44/71 (61.97%), Postives = 55/71 (77.46%), Query Frame = 1


Query: 1  MAEESAREKTQWPELVGVDYSTAATTIETENSHVKAIKFLVGSPMYLNFDPRRVRVVCDT 60
          M EE    K+ WPELVGVD+S AA  IETEN +VKA K L+GSP+ LN+DP RV V+C+T
Sbjct: 1  MDEEFDTRKSIWPELVGVDFSAAARIIETENPNVKATKILIGSPVILNYDPTRVWVICNT 60

Query: 61 QDKVVEMPYVG 72
          +DKVV++P VG
Sbjct: 61 EDKVVDIPRVG 71

[Close]

BLAST of Cucsa.255090 vs. TrEMBL
Match: A0A0A0L785_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G141900 PE=4 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 5.4e-13
Identity = 42/71 (59.15%), Postives = 52/71 (73.24%), Query Frame = 1


Query: 1  MAEESAREKTQWPELVGVDYSTAATTIETENSHVKAIKFLVGSPMYLNFDPRRVRVVCDT 60
          MAEESA+ KT+WPELV V++ TAA  IE EN  VKAIK LV SP   NFD  RV V C+ 
Sbjct: 1  MAEESAQRKTRWPELVFVNFCTAARIIEKENPDVKAIKILVDSPRIQNFDISRVWVDCNI 60

Query: 61 QDKVVEMPYVG 72
          +++VV++P VG
Sbjct: 61 EERVVKVPSVG 71

[Close]

BLAST of Cucsa.255090 vs. TrEMBL
Match: A0A0A0L8E6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G142910 PE=4 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 3.0e-11
Identity = 40/71 (56.34%), Postives = 48/71 (67.61%), Query Frame = 1


Query: 1  MAEESAREKTQWPELVGVDYSTAATTIETENSHVKAIKFLVGSPMYLNFDPRRVRVVCDT 60
          MAEESA  KT+WPELV V YS AA  IE EN  V+A+K L G+P  LNFD  RV V  + 
Sbjct: 1  MAEESAPRKTEWPELVFVKYSIAAAIIEKENPDVEAVKILSGTPRILNFDINRVWVDINV 60

Query: 61 QDKVVEMPYVG 72
          ++ VV+ P VG
Sbjct: 61 EEVVVKKPKVG 71

[Close]

BLAST of Cucsa.255090 vs. TrEMBL
Match: V4VRJ6_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10022906mg PE=4 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 2.0e-07
Identity = 32/63 (50.79%), Postives = 43/63 (68.25%), Query Frame = 1


Query: 9   KTQWPELVGVDYSTAATTIETENSHVKAIKFLVGSPMYLNFDPRRVRVVCDTQDKVVEMP 68
           K+ WPELVGV+   AA TI+ EN +V AI  L G+P+  +F   RVRVV D  +KVV++P
Sbjct: 57  KSSWPELVGVNGEIAAATIQKENPNVHAIIILEGTPVTQDFRCDRVRVVVDKYEKVVQVP 116

Query: 69  YVG 72
            +G
Sbjct: 117 RIG 119

[Close]

BLAST of Cucsa.255090 vs. NCBI nr
Match: gi|700201762|gb|KGN56895.1| (Protease inhibitor protein [Cucumis sativus])

HSP 1 Score: 146.7 bits (369), Expect = 1.5e-32
Identity = 71/71 (100.00%), Postives = 71/71 (100.00%), Query Frame = 1


Query: 1  MAEESAREKTQWPELVGVDYSTAATTIETENSHVKAIKFLVGSPMYLNFDPRRVRVVCDT 60
          MAEESAREKTQWPELVGVDYSTAATTIETENSHVKAIKFLVGSPMYLNFDPRRVRVVCDT
Sbjct: 1  MAEESAREKTQWPELVGVDYSTAATTIETENSHVKAIKFLVGSPMYLNFDPRRVRVVCDT 60

Query: 61 QDKVVEMPYVG 72
          QDKVVEMPYVG
Sbjct: 61 QDKVVEMPYVG 71

[Close]

BLAST of Cucsa.255090 vs. NCBI nr
Match: gi|700201763|gb|KGN56896.1| (hypothetical protein Csa_3G142410 [Cucumis sativus])

HSP 1 Score: 92.8 bits (229), Expect = 2.6e-16
Identity = 44/71 (61.97%), Postives = 55/71 (77.46%), Query Frame = 1


Query: 1  MAEESAREKTQWPELVGVDYSTAATTIETENSHVKAIKFLVGSPMYLNFDPRRVRVVCDT 60
          M EE    K+ WPELVGVD+S AA  IETEN +VKA K L+GSP+ LN+DP RV V+C+T
Sbjct: 1  MDEEFDTRKSIWPELVGVDFSAAARIIETENPNVKATKILIGSPVILNYDPTRVWVICNT 60

Query: 61 QDKVVEMPYVG 72
          +DKVV++P VG
Sbjct: 61 EDKVVDIPRVG 71

[Close]

BLAST of Cucsa.255090 vs. NCBI nr
Match: gi|700201761|gb|KGN56894.1| (hypothetical protein Csa_3G141900 [Cucumis sativus])

HSP 1 Score: 81.3 bits (199), Expect = 7.8e-13
Identity = 42/71 (59.15%), Postives = 52/71 (73.24%), Query Frame = 1


Query: 1  MAEESAREKTQWPELVGVDYSTAATTIETENSHVKAIKFLVGSPMYLNFDPRRVRVVCDT 60
          MAEESA+ KT+WPELV V++ TAA  IE EN  VKAIK LV SP   NFD  RV V C+ 
Sbjct: 1  MAEESAQRKTRWPELVFVNFCTAARIIEKENPDVKAIKILVDSPRIQNFDISRVWVDCNI 60

Query: 61 QDKVVEMPYVG 72
          +++VV++P VG
Sbjct: 61 EERVVKVPSVG 71

[Close]

BLAST of Cucsa.255090 vs. NCBI nr
Match: gi|700201764|gb|KGN56897.1| (hypothetical protein Csa_3G142910 [Cucumis sativus])

HSP 1 Score: 75.5 bits (184), Expect = 4.3e-11
Identity = 40/71 (56.34%), Postives = 48/71 (67.61%), Query Frame = 1


Query: 1  MAEESAREKTQWPELVGVDYSTAATTIETENSHVKAIKFLVGSPMYLNFDPRRVRVVCDT 60
          MAEESA  KT+WPELV V YS AA  IE EN  V+A+K L G+P  LNFD  RV V  + 
Sbjct: 1  MAEESAPRKTEWPELVFVKYSIAAAIIEKENPDVEAVKILSGTPRILNFDINRVWVDINV 60

Query: 61 QDKVVEMPYVG 72
          ++ VV+ P VG
Sbjct: 61 EEVVVKKPKVG 71

[Close]

BLAST of Cucsa.255090 vs. NCBI nr
Match: gi|567899842|ref|XP_006442409.1| (hypothetical protein CICLE_v10022906mg [Citrus clementina])

HSP 1 Score: 62.8 bits (151), Expect = 2.9e-07
Identity = 32/63 (50.79%), Postives = 43/63 (68.25%), Query Frame = 1


Query: 9   KTQWPELVGVDYSTAATTIETENSHVKAIKFLVGSPMYLNFDPRRVRVVCDTQDKVVEMP 68
           K+ WPELVGV+   AA TI+ EN +V AI  L G+P+  +F   RVRVV D  +KVV++P
Sbjct: 57  KSSWPELVGVNGEIAAATIQKENPNVHAIIILEGTPVTQDFRCDRVRVVVDKYEKVVQVP 116

Query: 69  YVG 72
            +G
Sbjct: 117 RIG 119

The following BLAST results are available for this feature:

BLAST of Cucsa.255090 vs. Swiss-Prot


Match Name	E-value	Identity	Description
HPI_HEVBR	4.2e-06	42.86	Protease inhibitor HPI OS=Hevea brasiliensis GN=PI1 PE=1 SV=2	[more]

BLAST of Cucsa.255090 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L4I0_CUCSA	1.1e-32	100.00	Protease inhibitor protein OS=Cucumis sativus GN=Csa_3G142400 PE=4 SV=1	[more]
A0A0A0L9Z4_CUCSA	1.8e-16	61.97	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G142410 PE=4 SV=1	[more]
A0A0A0L785_CUCSA	5.4e-13	59.15	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G141900 PE=4 SV=1	[more]
A0A0A0L8E6_CUCSA	3.0e-11	56.34	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G142910 PE=4 SV=1	[more]
V4VRJ6_9ROSI	2.0e-07	50.79	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10022906mg PE=4 SV=1	[more]

BLAST of Cucsa.255090 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700201762\|gb\|KGN56895.1\|	1.5e-32	100.00	Protease inhibitor protein [Cucumis sativus]	[more]
gi\|700201763\|gb\|KGN56896.1\|	2.6e-16	61.97	hypothetical protein Csa_3G142410 [Cucumis sativus]	[more]
gi\|700201761\|gb\|KGN56894.1\|	7.8e-13	59.15	hypothetical protein Csa_3G141900 [Cucumis sativus]	[more]
gi\|700201764\|gb\|KGN56897.1\|	4.3e-11	56.34	hypothetical protein Csa_3G142910 [Cucumis sativus]	[more]
gi\|567899842\|ref\|XP_006442409.1\|	2.9e-07	50.79	hypothetical protein CICLE_v10022906mg [Citrus clementina]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR000864	Prot_inh_pot1
IPR000864	Prot_inh_pot1

Vocabulary: **Molecular Function**
Term	Definition
GO:0004867	serine-type endopeptidase inhibitor activity
GO:0004867	serine-type endopeptidase inhibitor activity

Vocabulary: **Biological Process**
Term	Definition
GO:0009611	response to wounding
GO:0009611	response to wounding

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
biological_process	GO:0009611	response to wounding
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0008233	peptidase activity
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsa.255090.2	Cucsa.255090.2	mRNA
Cucsa.255090.1	Cucsa.255090.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PRODOM	PD002604		coord: 5..65 score: 5.
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PFAM	PF00280	potato_inhibit	coord: 8..65 score: 5.9
IPR000864	Proteinase inhibitor I13, potato inhibitor I	unknown	SSF54654	CI-2 family of serine protease inhibitors	coord: 8..65 score: 6.67
None	No IPR available	GENE3D	G3DSA:3.30.10.10		coord: 8..65 score: 5.3
None	No IPR available	PANTHER	PTHR33091	FAMILY NOT NAMED	coord: 8..65 score: 4.5

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cucsa.255090	Melon (DHL92) v3.6.1	cgymedB429
Cucsa.255090	Melon (DHL92) v3.6.1	cgymedB432
Cucsa.255090	Silver-seed gourd	carcgyB1077
Cucsa.255090	Silver-seed gourd	carcgyB0269
Cucsa.255090	Silver-seed gourd	carcgyB0276
Cucsa.255090	Silver-seed gourd	carcgyB1190
Cucsa.255090	Cucumber (Chinese Long) v3	cgycucB387
Cucsa.255090	Watermelon (97103) v2	cgywmbB452
Cucsa.255090	Watermelon (97103) v2	cgywmbB453
Cucsa.255090	Wax gourd	cgywgoB510
Cucsa.255090	Wax gourd	cgywgoB532
Cucsa.255090	Cucumber (Gy14) v1	cgycgyB133
Cucsa.255090	Cucurbita maxima (Rimu)	cgycmaB0709
Cucsa.255090	Cucurbita maxima (Rimu)	cgycmaB0727
Cucsa.255090	Cucurbita moschata (Rifu)	cgycmoB0700
Cucsa.255090	Cucurbita moschata (Rifu)	cgycmoB0718
Cucsa.255090	Wild cucumber (PI 183967)	cgycpiB383
Cucsa.255090	Wild cucumber (PI 183967)	cgycpiB378
Cucsa.255090	Cucumber (Chinese Long) v2	cgycuB362
Cucsa.255090	Cucumber (Chinese Long) v2	cgycuB366
Cucsa.255090	Melon (DHL92) v3.5.1	cgymeB434
Cucsa.255090	Melon (DHL92) v3.5.1	cgymeB439
Cucsa.255090	Watermelon (Charleston Gray)	cgywcgB451
Cucsa.255090	Watermelon (Charleston Gray)	cgywcgB452
Cucsa.255090	Watermelon (Charleston Gray)	cgywcgB457
Cucsa.255090	Watermelon (97103) v1	cgywmB475
Cucsa.255090	Watermelon (97103) v1	cgywmB479
Cucsa.255090	Watermelon (97103) v1	cgywmB483
Cucsa.255090	Cucurbita pepo (Zucchini)	cgycpeB0682
Cucsa.255090	Cucurbita pepo (Zucchini)	cgycpeB0687
Cucsa.255090	Bottle gourd (USVL1VR-Ls)	cgylsiB412
Cucsa.255090	Bottle gourd (USVL1VR-Ls)	cgylsiB415