Cucsa.254330 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.254330 vs. Swiss-Prot
Match: PP312_ARATH (Pentatricopeptide repeat-containing protein At4g14850 OS=Arabidopsis thaliana GN=LOI1 PE=1 SV=1)

HSP 1 Score: 760.4 bits (1962), Expect = 1.5e-218
Identity = 372/605 (61.49%), Postives = 469/605 (77.52%), Query Frame = 1

Query: 1   MPFLSQNSLASVVELAVSVRSSLLGRAAHAQILKTLKTPFPAFLYNHLVNMYAKLDHLNS 60
           M  LS ++L  +++ A+S  S  LGR  HA+I+KTL +P P FL N+L+NMY+KLDH  S
Sbjct: 1   MSLLSADALGLLLKNAISASSMRLGRVVHARIVKTLDSPPPPFLANYLINMYSKLDHPES 60

Query: 61  AKLILELAPCRSVVTWTALIAGSVQNGCFVSALLHFSDMLSDCVRPNDFTFPCVLKASTG 120
           A+L+L L P R+VV+WT+LI+G  QNG F +AL+ F +M  + V PNDFTFPC  KA   
Sbjct: 61  ARLVLRLTPARNVVSWTSLISGLAQNGHFSTALVEFFEMRREGVVPNDFTFPCAFKAVAS 120

Query: 121 LRMDTTGKQLHALAVKEGLINDVFVGCSVFDMYSKLGFLNDAYKVFDEMPHRNLETWNAY 180
           LR+  TGKQ+HALAVK G I DVFVGCS FDMY K    +DA K+FDE+P RNLETWNA+
Sbjct: 121 LRLPVTGKQIHALAVKCGRILDVFVGCSAFDMYCKTRLRDDARKLFDEIPERNLETWNAF 180

Query: 181 ISNSVLHGRPEDSVIAFIELLRVGGKPDSITFCAFLNACSDKLGLGPGCQLHGFIIRSGY 240
           ISNSV  GRP +++ AFIE  R+ G P+SITFCAFLNACSD L L  G QLHG ++RSG+
Sbjct: 181 ISNSVTDGRPREAIEAFIEFRRIDGHPNSITFCAFLNACSDWLHLNLGMQLHGLVLRSGF 240

Query: 241 GQNVSVSNGLIDFYGKCGEVECSEMVFDRMGERNSVSWSSLIAAYVQNNEEEKASCLFLR 300
             +VSV NGLIDFYGKC ++  SE++F  MG +N+VSW SL+AAYVQN+E+EKAS L+LR
Sbjct: 241 DTDVSVCNGLIDFYGKCKQIRSSEIIFTEMGTKNAVSWCSLVAAYVQNHEDEKASVLYLR 300

Query: 301 ARKEDIEPTDFMVSSVLCACAGLSEIEFGRSVQALAVKACVEQNIFVASALVDMYGKCGS 360
           +RK+ +E +DFM+SSVL ACAG++ +E GRS+ A AVKACVE+ IFV SALVDMYGKCG 
Sbjct: 301 SRKDIVETSDFMISSVLSACAGMAGLELGRSIHAHAVKACVERTIFVGSALVDMYGKCGC 360

Query: 361 IDNAEQAFNAMPERNLVSWNALLGGYAHQGHANKAVALLEEMT-SAAGIVPSYVSLICAL 420
           I+++EQAF+ MPE+NLV+ N+L+GGYAHQG  + A+AL EEM     G  P+Y++ +  L
Sbjct: 361 IEDSEQAFDEMPEKNLVTRNSLIGGYAHQGQVDMALALFEEMAPRGCGPTPNYMTFVSLL 420

Query: 421 SACSRAGDLKTGMKIFESMKERYGVEPGPEHYACLVDLLGRAGMVECAYDFIKRMPFPPT 480
           SACSRAG ++ GMKIF+SM+  YG+EPG EHY+C+VD+LGRAGMVE AY+FIK+MP  PT
Sbjct: 421 SACSRAGAVENGMKIFDSMRSTYGIEPGAEHYSCIVDMLGRAGMVERAYEFIKKMPIQPT 480

Query: 481 ISIWGALLGACRMHGKPELGKLAAEKLFELDPKDSGNHVVLSNMFAATGRWEEVTVVRNE 540
           IS+WGAL  ACRMHGKP+LG LAAE LF+LDPKDSGNHV+LSN FAA GRW E   VR E
Sbjct: 481 ISVWGALQNACRMHGKPQLGLLAAENLFKLDPKDSGNHVLLSNTFAAAGRWAEANTVREE 540

Query: 541 MKEVGIKKGAGFSWITVDSRIHMFQAKDKSHEKDPEIQDILGKLRKEMQDAAGCIADPNY 600
           +K VGIKKGAG+SWITV +++H FQAKD+SH  + EIQ  L KLR EM +AAG   D   
Sbjct: 541 LKGVGIKKGAGYSWITVKNQVHAFQAKDRSHILNKEIQTTLAKLRNEM-EAAGYKPDLKL 600

Query: 601 ALFEV 605
           +L+++
Sbjct: 601 SLYDL 604

BLAST of Cucsa.254330 vs. Swiss-Prot
Match: PP373_ARATH (Putative pentatricopeptide repeat-containing protein At5g09950 OS=Arabidopsis thaliana GN=PCMP-H35 PE=3 SV=1)

HSP 1 Score: 418.7 bits (1075), Expect = 1.1e-115
Identity = 226/585 (38.63%), Postives = 342/585 (58.46%), Query Frame = 1

Query: 25  GRAAHAQILKTLKTPFPAFLYNHLVNMYAKLDHLNSAKLILELAPCRSVVTWTALIAGSV 84
           GR  H  ++ T    F   + N LVNMYAK   +  A+ +      +  V+W ++I G  
Sbjct: 332 GREVHGHVITTGLVDFMVGIGNGLVNMYAKCGSIADARRVFYFMTDKDSVSWNSMITGLD 391

Query: 85  QNGCFVSALLHFSDMLSDCVRPNDFTFPCVLKASTGLRMDTTGKQLHALAVKEGLINDVF 144
           QNGCF+ A+  +  M    + P  FT    L +   L+    G+Q+H  ++K G+  +V 
Sbjct: 392 QNGCFIEAVERYKSMRRHDILPGSFTLISSLSSCASLKWAKLGQQIHGESLKLGIDLNVS 451

Query: 145 VGCSVFDMYSKLGFLNDAYKVFDEMPHRNLETWNAYISNSVLHGRP-EDSVIAFIELLRV 204
           V  ++  +Y++ G+LN+  K+F  MP  +  +WN+ I       R   ++V+ F+   R 
Sbjct: 452 VSNALMTLYAETGYLNECRKIFSSMPEHDQVSWNSIIGALARSERSLPEAVVCFLNAQRA 511

Query: 205 GGKPDSITFCAFLNACSDKLGLGP-GCQLHGFIIRSGYGQNVSVSNGLIDFYGKCGEVEC 264
           G K + ITF + L+A S  L  G  G Q+HG  +++      +  N LI  YGKCGE++ 
Sbjct: 512 GQKLNRITFSSVLSAVSS-LSFGELGKQIHGLALKNNIADEATTENALIACYGKCGEMDG 571

Query: 265 SEMVFDRMGER-NSVSWSSLIAAYVQNNEEEKASCLFLRARKEDIEPTDFMVSSVLCACA 324
            E +F RM ER ++V+W+S+I+ Y+ N    KA  L     +       FM ++VL A A
Sbjct: 572 CEKIFSRMAERRDNVTWNSMISGYIHNELLAKALDLVWFMLQTGQRLDSFMYATVLSAFA 631

Query: 325 GLSEIEFGRSVQALAVKACVEQNIFVASALVDMYGKCGSIDNAEQAFNAMPERNLVSWNA 384
            ++ +E G  V A +V+AC+E ++ V SALVDMY KCG +D A + FN MP RN  SWN+
Sbjct: 632 SVATLERGMEVHACSVRACLESDVVVGSALVDMYSKCGRLDYALRFFNTMPVRNSYSWNS 691

Query: 385 LLGGYAHQGHANKAVALLEEMTSAAGIVPSYVSLICALSACSRAGDLKTGMKIFESMKER 444
           ++ GYA  G   +A+ L E M       P +V+ +  LSACS AG L+ G K FESM + 
Sbjct: 692 MISGYARHGQGEEALKLFETMKLDGQTPPDHVTFVGVLSACSHAGLLEEGFKHFESMSDS 751

Query: 445 YGVEPGPEHYACLVDLLGRAGMVECAYDFIKRMPFPPTISIWGALLGA-CRMHG-KPELG 504
           YG+ P  EH++C+ D+LGRAG ++   DFI++MP  P + IW  +LGA CR +G K ELG
Sbjct: 752 YGLAPRIEHFSCMADVLGRAGELDKLEDFIEKMPMKPNVLIWRTVLGACCRANGRKAELG 811

Query: 505 KLAAEKLFELDPKDSGNHVVLSNMFAATGRWEEVTVVRNEMKEVGIKKGAGFSWITVDSR 564
           K AAE LF+L+P+++ N+V+L NM+AA GRWE++   R +MK+  +KK AG+SW+T+   
Sbjct: 812 KKAAEMLFQLEPENAVNYVLLGNMYAAGGRWEDLVKARKKMKDADVKKEAGYSWVTMKDG 871

Query: 565 IHMFQAKDKSHEKDPEIQDILGKLRKEMQDAAGCIADPNYALFEV 605
           +HMF A DKSH     I   L +L ++M+D AG +    +AL+++
Sbjct: 872 VHMFVAGDKSHPDADVIYKKLKELNRKMRD-AGYVPQTGFALYDL 914

HSP 2 Score: 178.7 bits (452), Expect = 1.9e-43
Identity = 145/497 (29.18%), Postives = 240/497 (48.29%), Query Frame = 1

Query: 43  FLYNHLVNMYAKLDHLNSAKLILELAPCRSVVTWTALIAGSVQNGCFVSALLHFSDMLSD 102
           F+ + LV+ +AK   L+ A+ +      R+ VT   L+ G V+      A   F DM S 
Sbjct: 244 FVGSGLVSAFAKSGSLSYARKVFNQMETRNAVTLNGLMVGLVRQKWGEEATKLFMDMNSM 303

Query: 103 C-VRPNDF-----TFP-CVLKASTGLRMDTTGKQLHALAVKEGLIND-VFVGCSVFDMYS 162
             V P  +     +FP   L    GL+    G+++H   +  GL++  V +G  + +MY+
Sbjct: 304 IDVSPESYVILLSSFPEYSLAEEVGLKK---GREVHGHVITTGLVDFMVGIGNGLVNMYA 363

Query: 163 KLGFLNDAYKVFDEMPHRNLETWNAYISNSVLHGRPEDSVIAFIELLRVGGKPDSITFCA 222
           K G + DA +VF  M  ++  +WN+ I+    +G   ++V  +  + R    P S T  +
Sbjct: 364 KCGSIADARRVFYFMTDKDSVSWNSMITGLDQNGCFIEAVERYKSMRRHDILPGSFTLIS 423

Query: 223 FLNACSDKLGLGPGCQLHGFIIRSGYGQNVSVSNGLIDFYGKCGEVECSEMVFDRMGERN 282
            L++C+       G Q+HG  ++ G   NVSVSN L+  Y + G +     +F  M E +
Sbjct: 424 SLSSCASLKWAKLGQQIHGESLKLGIDLNVSVSNALMTLYAETGYLNECRKIFSSMPEHD 483

Query: 283 SVSWSSLIAAYVQNNEE-EKASCLFLRARKEDIEPTDFMVSSVLCACAGLSEIEFGRSVQ 342
            VSW+S+I A  ++     +A   FL A++   +      SSVL A + LS  E G+ + 
Sbjct: 484 QVSWNSIIGALARSERSLPEAVVCFLNAQRAGQKLNRITFSSVLSAVSSLSFGELGKQIH 543

Query: 343 ALAVKACVEQNIFVASALVDMYGKCGSIDNAEQAFNAMPE-RNLVSWNALLGGYAHQGHA 402
            LA+K  +       +AL+  YGKCG +D  E+ F+ M E R+ V+WN+++ GY H    
Sbjct: 544 GLALKNNIADEATTENALIACYGKCGEMDGCEKIFSRMAERRDNVTWNSMISGYIHNELL 603

Query: 403 NKAVALLEEMTSAAGIVPSYVSLICALSACSRAGDLKTGMKIFESMKERYGVEPGPEHYA 462
            KA+ L+  M      + S++     LSA +    L+ GM++  +   R  +E      +
Sbjct: 604 AKALDLVWFMLQTGQRLDSFM-YATVLSAFASVATLERGMEV-HACSVRACLESDVVVGS 663

Query: 463 CLVDLLGRAGMVECAYDFIKRMPFPPTISIWGALLGACRMHGKPELGKLAAEKLFE---L 522
            LVD+  + G ++ A  F   MP   + S W +++     HG+ E     A KLFE   L
Sbjct: 664 ALVDMYSKCGRLDYALRFFNTMPVRNSYS-WNSMISGYARHGQGE----EALKLFETMKL 723

Query: 523 DPKDSGNHVVLSNMFAA 527
           D +   +HV    + +A
Sbjct: 724 DGQTPPDHVTFVGVLSA 730

HSP 3 Score: 150.2 bits (378), Expect = 7.2e-35
Identity = 132/493 (26.77%), Postives = 232/493 (47.06%), Query Frame = 1

Query: 11  SVVELAVSVRSSLLGRAAHAQILKTLKTPFPAFLYNHLVNMYAKLDHLNSAKLILELAPC 70
           S V+  V  R +   R  H+++ K  +     +L N+L+N Y +     SA+ + +  P 
Sbjct: 8   SFVQSCVGHRGA--ARFFHSRLYKN-RLDKDVYLCNNLINAYLETGDSVSARKVFDEMPL 67

Query: 71  RSVVTWTALIAGSVQNGCFVSALLHFSDMLSDCVRPNDFTFPCVLKA-----STGLRMDT 130
           R+ V+W  +++G  +NG    AL+   DM+ + +  N + F  VL+A     S G+    
Sbjct: 68  RNCVSWACIVSGYSRNGEHKEALVFLRDMVKEGIFSNQYAFVSVLRACQEIGSVGILF-- 127

Query: 131 TGKQLHALAVKEGLINDVFVGCSVFDMYSK-LGFLNDAYKVFDEMPHRNLETWNAYISNS 190
            G+Q+H L  K     D  V   +  MY K +G +  A   F ++  +N  +WN+ IS  
Sbjct: 128 -GRQIHGLMFKLSYAVDAVVSNVLISMYWKCIGSVGYALCAFGDIEVKNSVSWNSIISVY 187

Query: 191 VLHGRPEDSVIAFIELLRVGGKPDSITFCAFLN-ACS-DKLGLGPGCQLHGFIIRSGYGQ 250
              G    +   F  +   G +P   TF + +  ACS  +  +    Q+   I +SG   
Sbjct: 188 SQAGDQRSAFRIFSSMQYDGSRPTEYTFGSLVTTACSLTEPDVRLLEQIMCTIQKSGLLT 247

Query: 251 NVSVSNGLIDFYGKCGEVECSEMVFDRMGERNSVSWSSLIAAYVQNNEEEKASCLFLRAR 310
           ++ V +GL+  + K G +  +  VF++M  RN+V+ + L+   V+    E+A+ LF+   
Sbjct: 248 DLFVGSGLVSAFAKSGSLSYARKVFNQMETRNAVTLNGLMVGLVRQKWGEEATKLFMDMN 307

Query: 311 KE-DIEPTDFMVSSVLCACAGLSE---IEFGRSVQALAVKA-CVEQNIFVASALVDMYGK 370
              D+ P  +++         L+E   ++ GR V    +    V+  + + + LV+MY K
Sbjct: 308 SMIDVSPESYVILLSSFPEYSLAEEVGLKKGREVHGHVITTGLVDFMVGIGNGLVNMYAK 367

Query: 371 CGSIDNAEQAFNAMPERNLVSWNALLGGYAHQGHANKAVALLEEMTSAAGIVPSYVSLIC 430
           CGSI +A + F  M +++ VSWN+++ G    G   +AV   + M     I+P   +LI 
Sbjct: 368 CGSIADARRVFYFMTDKDSVSWNSMITGLDQNGCFIEAVERYKSM-RRHDILPGSFTLIS 427

Query: 431 ALSACSRAGDLKTGMKIF-ESMKERYGVEPGPEHYACLVDLLGRAGMVECAYDFIKRMPF 490
           +LS+C+     K G +I  ES+K   G++        L+ L    G +         MP 
Sbjct: 428 SLSSCASLKWAKLGQQIHGESLK--LGIDLNVSVSNALMTLYAETGYLNECRKIFSSMPE 487

HSP 4 Score: 97.4 bits (241), Expect = 5.6e-19
Identity = 82/323 (25.39%), Postives = 142/323 (43.96%), Query Frame = 1

Query: 232 HGFIIRSGYGQNVSVSNGLIDFYGKCGEVECSEMVFDRMGERNSVSWSSLIAAYVQNNEE 291
           H  + ++   ++V + N LI+ Y + G+   +  VFD M  RN VSW+ +++ Y +N E 
Sbjct: 24  HSRLYKNRLDKDVYLCNNLINAYLETGDSVSARKVFDEMPLRNCVSWACIVSGYSRNGEH 83

Query: 292 EKASCLFLRARKEDIEPTDFMVSSVLCAC--AGLSEIEFGRSVQALAVKACVEQNIFVAS 351
           ++A        KE I    +   SVL AC   G   I FGR +  L  K     +  V++
Sbjct: 84  KEALVFLRDMVKEGIFSNQYAFVSVLRACQEIGSVGILFGRQIHGLMFKLSYAVDAVVSN 143

Query: 352 ALVDMYGKC-GSIDNAEQAFNAMPERNLVSWNALLGGYAHQGHANKAVALLEEMTSAAGI 411
            L+ MY KC GS+  A  AF  +  +N VSWN+++  Y+  G    A  +   M      
Sbjct: 144 VLISMYWKCIGSVGYALCAFGDIEVKNSVSWNSIISVYSQAGDQRSAFRIFSSMQYDGSR 203

Query: 412 VPSYVSLICALSACSRAGDLKTGMKIFESMK---ERYGVEPGPEHYACLVDLLGRAGMVE 471
              Y       +ACS     +  +++ E +    ++ G+       + LV    ++G + 
Sbjct: 204 PTEYTFGSLVTTACSLT---EPDVRLLEQIMCTIQKSGLLTDLFVGSGLVSAFAKSGSLS 263

Query: 472 CAYDFIKRMPFPPTISIWGALLGACRMHGKPELGKLAAEKLFELDPKDSGNHVVLSNMFA 531
            A     +M     +++ G ++G  R     E  KL  +    +D       ++LS+   
Sbjct: 264 YARKVFNQMETRNAVTLNGLMVGLVRQKWGEEATKLFMDMNSMIDVSPESYVILLSS--- 323

Query: 532 ATGRWEEVTVVRNEMKEVGIKKG 549
               + E ++     +EVG+KKG
Sbjct: 324 ----FPEYSLA----EEVGLKKG 332

BLAST of Cucsa.254330 vs. Swiss-Prot
Match: PPR32_ARATH (Pentatricopeptide repeat-containing protein At1g11290, chloroplastic OS=Arabidopsis thaliana GN=PCMP-H40 PE=2 SV=1)

HSP 1 Score: 383.6 bits (984), Expect = 3.9e-105
Identity = 205/578 (35.47%), Postives = 328/578 (56.75%), Query Frame = 1

Query: 24  LGRAAHAQILKTLKTPFPAFLYNHLVNMYAKLDHLNSAKLILELAPCRSVVTWTALIAGS 83
           +G+  H  ++K+       F    L NMYAK   +N A+ + +  P R +V+W  ++AG 
Sbjct: 153 VGKEIHGLLVKS-GFSLDLFAMTGLENMYAKCRQVNEARKVFDRMPERDLVSWNTIVAGY 212

Query: 84  VQNGCFVSALLHFSDMLSDCVRPNDFTFPCVLKASTGLRMDTTGKQLHALAVKEGLINDV 143
            QNG    AL     M  + ++P+  T   VL A + LR+ + GK++H  A++ G  + V
Sbjct: 213 SQNGMARMALEMVKSMCEENLKPSFITIVSVLPAVSALRLISVGKEIHGYAMRSGFDSLV 272

Query: 144 FVGCSVFDMYSKLGFLNDAYKVFDEMPHRNLETWNAYISNSVLHGRPEDSVIAFIELLRV 203
            +  ++ DMY+K G L  A ++FD M  RN+ +WN+ I   V +  P+++++ F ++L  
Sbjct: 273 NISTALVDMYAKCGSLETARQLFDGMLERNVVSWNSMIDAYVQNENPKEAMLIFQKMLDE 332

Query: 204 GGKPDSITFCAFLNACSDKLGLGPGCQLHGFIIRSGYGQNVSVSNGLIDFYGKCGEVECS 263
           G KP  ++    L+AC+D   L  G  +H   +  G  +NVSV N LI  Y KC EV+ +
Sbjct: 333 GVKPTDVSVMGALHACADLGDLERGRFIHKLSVELGLDRNVSVVNSLISMYCKCKEVDTA 392

Query: 264 EMVFDRMGERNSVSWSSLIAAYVQNNEEEKASCLFLRARKEDIEPTDFMVSSVLCACAGL 323
             +F ++  R  VSW+++I  + QN     A   F + R   ++P  F   SV+ A A L
Sbjct: 393 ASMFGKLQSRTLVSWNAMILGFAQNGRPIDALNYFSQMRSRTVKPDTFTYVSVITAIAEL 452

Query: 324 SEIEFGRSVQALAVKACVEQNIFVASALVDMYGKCGSIDNAEQAFNAMPERNLVSWNALL 383
           S     + +  + +++C+++N+FV +ALVDMY KCG+I  A   F+ M ER++ +WNA++
Sbjct: 453 SITHHAKWIHGVVMRSCLDKNVFVTTALVDMYAKCGAIMIARLIFDMMSERHVTTWNAMI 512

Query: 384 GGYAHQGHANKAVALLEEMTSAAGIVPSYVSLICALSACSRAGDLKTGMKIFESMKERYG 443
            GY   G    A+ L EEM     I P+ V+ +  +SACS +G ++ G+K F  MKE Y 
Sbjct: 513 DGYGTHGFGKAALELFEEMQKGT-IKPNGVTFLSVISACSHSGLVEAGLKCFYMMKENYS 572

Query: 444 VEPGPEHYACLVDLLGRAGMVECAYDFIKRMPFPPTISIWGALLGACRMHGKPELGKLAA 503
           +E   +HY  +VDLLGRAG +  A+DFI +MP  P ++++GA+LGAC++H      + AA
Sbjct: 573 IELSMDHYGAMVDLLGRAGRLNEAWDFIMQMPVKPAVNVYGAMLGACQIHKNVNFAEKAA 632

Query: 504 EKLFELDPKDSGNHVVLSNMFAATGRWEEVTVVRNEMKEVGIKKGAGFSWITVDSRIHMF 563
           E+LFEL+P D G HV+L+N++ A   WE+V  VR  M   G++K  G S + + + +H F
Sbjct: 633 ERLFELNPDDGGYHVLLANIYRAASMWEKVGQVRVSMLRQGLRKTPGCSMVEIKNEVHSF 692

Query: 564 QAKDKSHEKDPEIQDILGKLRKEMQDAAGCIADPNYAL 602
            +   +H    +I   L KL   +++ AG + D N  L
Sbjct: 693 FSGSTAHPDSKKIYAFLEKLICHIKE-AGYVPDTNLVL 727

HSP 2 Score: 226.1 bits (575), Expect = 1.0e-57
Identity = 147/469 (31.34%), Postives = 233/469 (49.68%), Query Frame = 1

Query: 43  FLYNHLVNMYAKLDHLNSAKLILELAPCRSVVTWTALIAGSVQNGCFVSALLHFSDMLSD 102
           F    LV+++ +   ++ A  + E    +  V +  ++ G  +      AL  F  M  D
Sbjct: 70  FFQTKLVSLFCRYGSVDEAARVFEPIDSKLNVLYHTMLKGFAKVSDLDKALQFFVRMRYD 129

Query: 103 CVRPNDFTFPCVLKASTGLRMDTTGKQLHALAVKEGLINDVFVGCSVFDMYSKLGFLNDA 162
            V P  + F  +LK          GK++H L VK G   D+F    + +MY+K   +N+A
Sbjct: 130 DVEPVVYNFTYLLKVCGDEAELRVGKEIHGLLVKSGFSLDLFAMTGLENMYAKCRQVNEA 189

Query: 163 YKVFDEMPHRNLETWNAYISNSVLHGRPEDSVIAFIELLRVGGKPDSITFCAFLNACSDK 222
            KVFD MP R+L +WN  ++    +G    ++     +     KP  IT  + L A S  
Sbjct: 190 RKVFDRMPERDLVSWNTIVAGYSQNGMARMALEMVKSMCEENLKPSFITIVSVLPAVSAL 249

Query: 223 LGLGPGCQLHGFIIRSGYGQNVSVSNGLIDFYGKCGEVECSEMVFDRMGERNSVSWSSLI 282
             +  G ++HG+ +RSG+   V++S  L+D Y KCG +E +  +FD M ERN VSW+S+I
Sbjct: 250 RLISVGKEIHGYAMRSGFDSLVNISTALVDMYAKCGSLETARQLFDGMLERNVVSWNSMI 309

Query: 283 AAYVQNNEEEKASCLFLRARKEDIEPTDFMVSSVLCACAGLSEIEFGRSVQALAVKACVE 342
            AYVQN   ++A  +F +   E ++PTD  V   L ACA L ++E GR +  L+V+  ++
Sbjct: 310 DAYVQNENPKEAMLIFQKMLDEGVKPTDVSVMGALHACADLGDLERGRFIHKLSVELGLD 369

Query: 343 QNIFVASALVDMYGKCGSIDNAEQAFNAMPERNLVSWNALLGGYAHQGHANKAVALLEEM 402
           +N+ V ++L+ MY KC  +D A   F  +  R LVSWNA++ G+A  G    A+    +M
Sbjct: 370 RNVSVVNSLISMYCKCKEVDTAASMFGKLQSRTLVSWNAMILGFAQNGRPIDALNYFSQM 429

Query: 403 TSAAGIVP---SYVSLICALSACSRAGDLKTGMKIFESMKERYGVEPGPEHYACLVDLLG 462
            S   + P   +YVS+I A++  S    +    K    +  R  ++        LVD+  
Sbjct: 430 RSRT-VKPDTFTYVSVITAIAELS----ITHHAKWIHGVVMRSCLDKNVFVTTALVDMYA 489

Query: 463 RAGMVECAYDFIKRMPFPPTISIWGALLGACRMHGKPELGKLAAEKLFE 509
           + G +  A   I  M     ++ W A++     HG    GK A E LFE
Sbjct: 490 KCGAIMIA-RLIFDMMSERHVTTWNAMIDGYGTHG---FGKAALE-LFE 528

BLAST of Cucsa.254330 vs. Swiss-Prot
Match: PP280_ARATH (Pentatricopeptide repeat-containing protein At3g53360, mitochondrial OS=Arabidopsis thaliana GN=PCMP-E86 PE=2 SV=1)

HSP 1 Score: 372.5 bits (955), Expect = 9.0e-102
Identity = 195/586 (33.28%), Postives = 332/586 (56.66%), Query Frame = 1

Query: 6   QNSLASVVELAVSVRSSLLGRAAHAQILKTLKTPFPAFLYNHLVNMYAKLDHLNSAKLIL 65
           Q +  S+++   S     LG+  HAQ++K L++       N L+ MY + + ++ A  + 
Sbjct: 168 QFAFGSIIKACASSSDVGLGKQLHAQVIK-LESSSHLIAQNALIAMYVRFNQMSDASRVF 227

Query: 66  ELAPCRSVVTWTALIAGSVQNGCFVSALLHFSDMLSDCV-RPNDFTFPCVLKASTGLRMD 125
              P + +++W+++IAG  Q G    AL H  +MLS  V  PN++ F   LKA + L   
Sbjct: 228 YGIPMKDLISWSSIIAGFSQLGFEFEALSHLKEMLSFGVFHPNEYIFGSSLKACSSLLRP 287

Query: 126 TTGKQLHALAVKEGLINDVFVGCSVFDMYSKLGFLNDAYKVFDEMPHRNLETWNAYISNS 185
             G Q+H L +K  L  +   GCS+ DMY++ GFLN A +VFD++   +  +WN  I+  
Sbjct: 288 DYGSQIHGLCIKSELAGNAIAGCSLCDMYARCGFLNSARRVFDQIERPDTASWNVIIAGL 347

Query: 186 VLHGRPEDSVIAFIELLRVGGKPDSITFCAFLNACSDKLGLGPGCQLHGFIIRSGYGQNV 245
             +G  +++V  F ++   G  PD+I+  + L A +  + L  G Q+H +II+ G+  ++
Sbjct: 348 ANNGYADEAVSVFSQMRSSGFIPDAISLRSLLCAQTKPMALSQGMQIHSYIIKWGFLADL 407

Query: 246 SVSNGLIDFYGKCGEVECSEMVF-DRMGERNSVSWSSLIAAYVQNNEEEKASCLFLRARK 305
           +V N L+  Y  C ++ C   +F D     +SVSW++++ A +Q+ +  +   LF     
Sbjct: 408 TVCNSLLTMYTFCSDLYCCFNLFEDFRNNADSVSWNTILTACLQHEQPVEMLRLFKLMLV 467

Query: 306 EDIEPTDFMVSSVLCACAGLSEIEFGRSVQALAVKACVEQNIFVASALVDMYGKCGSIDN 365
            + EP    + ++L  C  +S ++ G  V   ++K  +    F+ + L+DMY KCGS+  
Sbjct: 468 SECEPDHITMGNLLRGCVEISSLKLGSQVHCYSLKTGLAPEQFIKNGLIDMYAKCGSLGQ 527

Query: 366 AEQAFNAMPERNLVSWNALLGGYAHQGHANKAVALLEEMTSAAGIVPSYVSLICALSACS 425
           A + F++M  R++VSW+ L+ GYA  G   +A+ L +EM SA GI P++V+ +  L+ACS
Sbjct: 528 ARRIFDSMDNRDVVSWSTLIVGYAQSGFGEEALILFKEMKSA-GIEPNHVTFVGVLTACS 587

Query: 426 RAGDLKTGMKIFESMKERYGVEPGPEHYACLVDLLGRAGMVECAYDFIKRMPFPPTISIW 485
             G ++ G+K++ +M+  +G+ P  EH +C+VDLL RAG +  A  FI  M   P + +W
Sbjct: 588 HVGLVEEGLKLYATMQTEHGISPTKEHCSCVVDLLARAGRLNEAERFIDEMKLEPDVVVW 647

Query: 486 GALLGACRMHGKPELGKLAAEKLFELDPKDSGNHVVLSNMFAATGRWEEVTVVRNEMKEV 545
             LL AC+  G   L + AAE + ++DP +S  HV+L +M A++G WE   ++R+ MK+ 
Sbjct: 648 KTLLSACKTQGNVHLAQKAAENILKIDPFNSTAHVLLCSMHASSGNWENAALLRSSMKKH 707

Query: 546 GIKKGAGFSWITVDSRIHMFQAKDKSHEKDPEIQDILGKLRKEMQD 590
            +KK  G SWI ++ +IH+F A+D  H +  +I  +L  +  +M D
Sbjct: 708 DVKKIPGQSWIEIEDKIHIFFAEDIFHPERDDIYTVLHNIWSQMLD 751

HSP 2 Score: 161.4 bits (407), Expect = 3.1e-38
Identity = 108/417 (25.90%), Postives = 202/417 (48.44%), Query Frame = 1

Query: 110 TFPCVLKASTGLRMDTTGKQLHALAVKEGLINDVFVGCSVFDMYSKLGFLNDAYKVFDEM 169
           T+  ++ A +  R    G+++H   +      D  +   +  MY K G L DA +VFD M
Sbjct: 69  TYISLICACSSSRSLAQGRKIHDHILNSNCKYDTILNNHILSMYGKCGSLRDAREVFDFM 128

Query: 170 PHRNLETWNAYISNSVLHGRPEDSVIAFIELLRVGGKPDSITFCAFLNACSDKLGLGPGC 229
           P RNL ++ + I+    +G+  +++  ++++L+    PD   F + + AC+    +G G 
Sbjct: 129 PERNLVSYTSVITGYSQNGQGAEAIRLYLKMLQEDLVPDQFAFGSIIKACASSSDVGLGK 188

Query: 230 QLHGFIIRSGYGQNVSVSNGLIDFYGKCGEVECSEMVFDRMGERNSVSWSSLIAAYVQ-N 289
           QLH  +I+     ++   N LI  Y +  ++  +  VF  +  ++ +SWSS+IA + Q  
Sbjct: 189 QLHAQVIKLESSSHLIAQNALIAMYVRFNQMSDASRVFYGIPMKDLISWSSIIAGFSQLG 248

Query: 290 NEEEKASCLFLRARKEDIEPTDFMVSSVLCACAGLSEIEFGRSVQALAVKACVEQNIFVA 349
            E E  S L          P +++  S L AC+ L   ++G  +  L +K+ +  N    
Sbjct: 249 FEFEALSHLKEMLSFGVFHPNEYIFGSSLKACSSLLRPDYGSQIHGLCIKSELAGNAIAG 308

Query: 350 SALVDMYGKCGSIDNAEQAFNAMPERNLVSWNALLGGYAHQGHANKAVALLEEMTSAAGI 409
            +L DMY +CG +++A + F+ +   +  SWN ++ G A+ G+A++AV++  +M S +G 
Sbjct: 309 CSLCDMYARCGFLNSARRVFDQIERPDTASWNVIIAGLANNGYADEAVSVFSQMRS-SGF 368

Query: 410 VPSYVSLICALSACSRAGDLKTGMKIFESMKERYGVEPGPEHYACLVDLLGRAGMVECAY 469
           +P  +SL   L A ++   L  GM+I  S   ++G          L+ +      + C +
Sbjct: 369 IPDAISLRSLLCAQTKPMALSQGMQI-HSYIIKWGFLADLTVCNSLLTMYTFCSDLYCCF 428

Query: 470 DFIKRMPFPPTISIWGALLGACRMHGKP-ELGKLAAEKLF-ELDPKDSGNHVVLSNM 524
           +  +          W  +L AC  H +P E+ +L    L  E +P    +H+ + N+
Sbjct: 429 NLFEDFRNNADSVSWNTILTACLQHEQPVEMLRLFKLMLVSECEP----DHITMGNL 479

HSP 3 Score: 160.6 bits (405), Expect = 5.3e-38
Identity = 121/479 (25.26%), Postives = 213/479 (44.47%), Query Frame = 1

Query: 11  SVVELAVSVRSSLLGRAAHAQILKTLKTPFPAFLYNHLVNMYAKLDHLNSAKLILELAPC 70
           S++    S RS   GR  H  IL +    +   L NH+++MY K   L  A+ + +  P 
Sbjct: 72  SLICACSSSRSLAQGRKIHDHILNS-NCKYDTILNNHILSMYGKCGSLRDAREVFDFMPE 131

Query: 71  RSVVTWTALIAGSVQNGCFVSALLHFSDMLSDCVRPNDFTFPCVLKASTGLRMDTTGKQL 130
           R++V++T++I G  QNG    A+  +  ML + + P+ F F  ++KA         GKQL
Sbjct: 132 RNLVSYTSVITGYSQNGQGAEAIRLYLKMLQEDLVPDQFAFGSIIKACASSSDVGLGKQL 191

Query: 131 HALAVKEGLINDVFVGCSVFDMYSKLGFLNDAYKVFDEMPHRNLETWNAYISNSVLHGRP 190
           HA  +K    + +    ++  MY +   ++DA +VF  +P ++L +W++ I+     G  
Sbjct: 192 HAQVIKLESSSHLIAQNALIAMYVRFNQMSDASRVFYGIPMKDLISWSSIIAGFSQLGFE 251

Query: 191 EDSVIAFIELLRVG-GKPDSITFCAFLNACSDKLGLGPGCQLHGFIIRSGYGQNVSVSNG 250
            +++    E+L  G   P+   F + L ACS  L    G Q+HG  I+S    N      
Sbjct: 252 FEALSHLKEMLSFGVFHPNEYIFGSSLKACSSLLRPDYGSQIHGLCIKSELAGNAIAGCS 311

Query: 251 LIDFYGKCGEVECSEMVFDRMGERNSVSWSSLIAAYVQNNEEEKASCLFLRARKEDIEPT 310
           L D Y +CG +  +  VFD++   ++ SW+ +IA    N   ++A  +F + R     P 
Sbjct: 312 LCDMYARCGFLNSARRVFDQIERPDTASWNVIIAGLANNGYADEAVSVFSQMRSSGFIPD 371

Query: 311 DFMVSSVLCACAGLSEIEFGRSVQALAVKACVEQNIFVASALVDMYGKCGSIDNAEQAFN 370
              + S+LCA      +  G  + +  +K     ++ V ++L+ MY  C  +      F 
Sbjct: 372 AISLRSLLCAQTKPMALSQGMQIHSYIIKWGFLADLTVCNSLLTMYTFCSDLYCCFNLFE 431

Query: 371 AMPER-NLVSWNALLGGYAHQGHANKAVALLEEMTSAAGIVPSYVSLICALSACSRAGDL 430
                 + VSWN +L          + + L + M   +   P ++++   L  C     L
Sbjct: 432 DFRNNADSVSWNTILTACLQHEQPVEMLRLFKLML-VSECEPDHITMGNLLRGCVEISSL 491

Query: 431 KTGMKIFESMKERYGVEPGPEHYACLVDLLGRAGMVECAYDFIKRMPFPPTISIWGALL 488
           K G ++      + G+ P       L+D+  + G +  A      M     +S W  L+
Sbjct: 492 KLGSQV-HCYSLKTGLAPEQFIKNGLIDMYAKCGSLGQARRIFDSMDNRDVVS-WSTLI 546

BLAST of Cucsa.254330 vs. Swiss-Prot
Match: PP232_ARATH (Putative pentatricopeptide repeat-containing protein At3g15130 OS=Arabidopsis thaliana GN=PCMP-H86 PE=3 SV=1)

HSP 1 Score: 367.5 bits (942), Expect = 2.9e-100
Identity = 209/605 (34.55%), Postives = 329/605 (54.38%), Query Frame = 1

Query: 6   QNSLASVVELAVSVRSSLLGRAAHAQILKTLKTPFPAFLYNHLVNMYAKLDHLNSAKLIL 65
           + +L S++ +      S  G   H  +LK+  +       N+L++MY K      A  + 
Sbjct: 6   RQNLVSILRVCTRKGLSDQGGQVHCYLLKS-GSGLNLITSNYLIDMYCKCREPLMAYKVF 65

Query: 66  ELAPCRSVVTWTALIAGSVQNGCFVSALLHFSDMLSDCVRPNDFTFPCVLKASTGLRMDT 125
           +  P R+VV+W+AL++G V NG    +L  FS+M    + PN+FTF   LKA   L    
Sbjct: 66  DSMPERNVVSWSALMSGHVLNGDLKGSLSLFSEMGRQGIYPNEFTFSTNLKACGLLNALE 125

Query: 126 TGKQLHALAVKEGLINDVFVGCSVFDMYSKLGFLNDAYKVFDEMPHRNLETWNAYISNSV 185
            G Q+H   +K G    V VG S+ DMYSK G +N+A KVF  +  R+L +WNA I+  V
Sbjct: 126 KGLQIHGFCLKIGFEMMVEVGNSLVDMYSKCGRINEAEKVFRRIVDRSLISWNAMIAGFV 185

Query: 186 LHGRPEDSVIAF--IELLRVGGKPDSITFCAFLNACSDKLGLGPGCQLHGFIIRSGYG-- 245
             G    ++  F  ++   +  +PD  T  + L ACS    +  G Q+HGF++RSG+   
Sbjct: 186 HAGYGSKALDTFGMMQEANIKERPDEFTLTSLLKACSSTGMIYAGKQIHGFLVRSGFHCP 245

Query: 246 QNVSVSNGLIDFYGKCGEVECSEMVFDRMGERNSVSWSSLIAAYVQNNEEEKASCLFLRA 305
            + +++  L+D Y KCG +  +   FD++ E+  +SWSSLI  Y Q  E  +A  LF R 
Sbjct: 246 SSATITGSLVDLYVKCGYLFSARKAFDQIKEKTMISWSSLILGYAQEGEFVEAMGLFKRL 305

Query: 306 RKEDIEPTDFMVSSVLCACAGLSEIEFGRSVQALAVKACVEQNIFVASALVDMYGKCGSI 365
           ++ + +   F +SS++   A  + +  G+ +QALAVK        V +++VDMY KCG +
Sbjct: 306 QELNSQIDSFALSSIIGVFADFALLRQGKQMQALAVKLPSGLETSVLNSVVDMYLKCGLV 365

Query: 366 DNAEQAFNAMPERNLVSWNALLGGYAHQGHANKAVALLEEMTSAAGIVPSYVSLICALSA 425
           D AE+ F  M  ++++SW  ++ GY   G   K+V +  EM     I P  V  +  LSA
Sbjct: 366 DEAEKCFAEMQLKDVISWTVVITGYGKHGLGKKSVRIFYEMLRH-NIEPDEVCYLAVLSA 425

Query: 426 CSRAGDLKTGMKIFESMKERYGVEPGPEHYACLVDLLGRAGMVECAYDFIKRMPFPPTIS 485
           CS +G +K G ++F  + E +G++P  EHYAC+VDLLGRAG ++ A   I  MP  P + 
Sbjct: 426 CSHSGMIKEGEELFSKLLETHGIKPRVEHYACVVDLLGRAGRLKEAKHLIDTMPIKPNVG 485

Query: 486 IWGALLGACRMHGKPELGKLAAEKLFELDPKDSGNHVVLSNMFAATGRWEEVTVVRNEMK 545
           IW  LL  CR+HG  ELGK   + L  +D K+  N+V++SN++   G W E    R    
Sbjct: 486 IWQTLLSLCRVHGDIELGKEVGKILLRIDAKNPANYVMMSNLYGQAGYWNEQGNARELGN 545

Query: 546 EVGIKKGAGFSWITVDSRIHMFQAKDKSHEKDPEIQDILGKLRKEMQDAAGCIADPNYAL 605
             G+KK AG SW+ ++  +H F++ + SH   P IQ+ L +  + +++  G +    + L
Sbjct: 546 IKGLKKEAGMSWVEIEREVHFFRSGEDSHPLTPVIQETLKEAERRLREELGYVYGLKHEL 605

Query: 606 FEVSN 607
            ++ +
Sbjct: 606 HDIDD 608

BLAST of Cucsa.254330 vs. TrEMBL
Match: A0A0A0L4T8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G146650 PE=4 SV=1)

HSP 1 Score: 1223.0 bits (3163), Expect = 0.0e+00
Identity = 606/606 (100.00%), Postives = 606/606 (100.00%), Query Frame = 1

Query: 1   MPFLSQNSLASVVELAVSVRSSLLGRAAHAQILKTLKTPFPAFLYNHLVNMYAKLDHLNS 60
           MPFLSQNSLASVVELAVSVRSSLLGRAAHAQILKTLKTPFPAFLYNHLVNMYAKLDHLNS
Sbjct: 1   MPFLSQNSLASVVELAVSVRSSLLGRAAHAQILKTLKTPFPAFLYNHLVNMYAKLDHLNS 60

Query: 61  AKLILELAPCRSVVTWTALIAGSVQNGCFVSALLHFSDMLSDCVRPNDFTFPCVLKASTG 120
           AKLILELAPCRSVVTWTALIAGSVQNGCFVSALLHFSDMLSDCVRPNDFTFPCVLKASTG
Sbjct: 61  AKLILELAPCRSVVTWTALIAGSVQNGCFVSALLHFSDMLSDCVRPNDFTFPCVLKASTG 120

Query: 121 LRMDTTGKQLHALAVKEGLINDVFVGCSVFDMYSKLGFLNDAYKVFDEMPHRNLETWNAY 180
           LRMDTTGKQLHALAVKEGLINDVFVGCSVFDMYSKLGFLNDAYKVFDEMPHRNLETWNAY
Sbjct: 121 LRMDTTGKQLHALAVKEGLINDVFVGCSVFDMYSKLGFLNDAYKVFDEMPHRNLETWNAY 180

Query: 181 ISNSVLHGRPEDSVIAFIELLRVGGKPDSITFCAFLNACSDKLGLGPGCQLHGFIIRSGY 240
           ISNSVLHGRPEDSVIAFIELLRVGGKPDSITFCAFLNACSDKLGLGPGCQLHGFIIRSGY
Sbjct: 181 ISNSVLHGRPEDSVIAFIELLRVGGKPDSITFCAFLNACSDKLGLGPGCQLHGFIIRSGY 240

Query: 241 GQNVSVSNGLIDFYGKCGEVECSEMVFDRMGERNSVSWSSLIAAYVQNNEEEKASCLFLR 300
           GQNVSVSNGLIDFYGKCGEVECSEMVFDRMGERNSVSWSSLIAAYVQNNEEEKASCLFLR
Sbjct: 241 GQNVSVSNGLIDFYGKCGEVECSEMVFDRMGERNSVSWSSLIAAYVQNNEEEKASCLFLR 300

Query: 301 ARKEDIEPTDFMVSSVLCACAGLSEIEFGRSVQALAVKACVEQNIFVASALVDMYGKCGS 360
           ARKEDIEPTDFMVSSVLCACAGLSEIEFGRSVQALAVKACVEQNIFVASALVDMYGKCGS
Sbjct: 301 ARKEDIEPTDFMVSSVLCACAGLSEIEFGRSVQALAVKACVEQNIFVASALVDMYGKCGS 360

Query: 361 IDNAEQAFNAMPERNLVSWNALLGGYAHQGHANKAVALLEEMTSAAGIVPSYVSLICALS 420
           IDNAEQAFNAMPERNLVSWNALLGGYAHQGHANKAVALLEEMTSAAGIVPSYVSLICALS
Sbjct: 361 IDNAEQAFNAMPERNLVSWNALLGGYAHQGHANKAVALLEEMTSAAGIVPSYVSLICALS 420

Query: 421 ACSRAGDLKTGMKIFESMKERYGVEPGPEHYACLVDLLGRAGMVECAYDFIKRMPFPPTI 480
           ACSRAGDLKTGMKIFESMKERYGVEPGPEHYACLVDLLGRAGMVECAYDFIKRMPFPPTI
Sbjct: 421 ACSRAGDLKTGMKIFESMKERYGVEPGPEHYACLVDLLGRAGMVECAYDFIKRMPFPPTI 480

Query: 481 SIWGALLGACRMHGKPELGKLAAEKLFELDPKDSGNHVVLSNMFAATGRWEEVTVVRNEM 540
           SIWGALLGACRMHGKPELGKLAAEKLFELDPKDSGNHVVLSNMFAATGRWEEVTVVRNEM
Sbjct: 481 SIWGALLGACRMHGKPELGKLAAEKLFELDPKDSGNHVVLSNMFAATGRWEEVTVVRNEM 540

Query: 541 KEVGIKKGAGFSWITVDSRIHMFQAKDKSHEKDPEIQDILGKLRKEMQDAAGCIADPNYA 600
           KEVGIKKGAGFSWITVDSRIHMFQAKDKSHEKDPEIQDILGKLRKEMQDAAGCIADPNYA
Sbjct: 541 KEVGIKKGAGFSWITVDSRIHMFQAKDKSHEKDPEIQDILGKLRKEMQDAAGCIADPNYA 600

Query: 601 LFEVSN 607
           LFEVSN
Sbjct: 601 LFEVSN 606

BLAST of Cucsa.254330 vs. TrEMBL
Match: F6HDQ9_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_05s0020g03570 PE=4 SV=1)

HSP 1 Score: 827.8 bits (2137), Expect = 8.7e-237
Identity = 409/605 (67.60%), Postives = 488/605 (80.66%), Query Frame = 1

Query: 1   MPFLSQNSLASVVELAVSVRSSLLGRAAHAQILKTLKTPFPAFLYNHLVNMYAKLDHLNS 60
           MPFLS NSLAS+VE AVS + S LGRAAHAQI+KTL  P P+F+YNHLVNMY+KLD  NS
Sbjct: 1   MPFLSPNSLASLVESAVSTQCSRLGRAAHAQIIKTLDNPLPSFIYNHLVNMYSKLDRPNS 60

Query: 61  AKLILELAPCRSVVTWTALIAGSVQNGCFVSALLHFSDMLSDCVRPNDFTFPCVLKASTG 120
           A+L+L L P RSVVTWTALIAGSVQNG F SAL HFS+M  D ++PNDFTFPC  KAS  
Sbjct: 61  AQLLLSLTPNRSVVTWTALIAGSVQNGRFTSALFHFSNMRRDSIQPNDFTFPCAFKASGS 120

Query: 121 LRMDTTGKQLHALAVKEGLINDVFVGCSVFDMYSKLGFLNDAYKVFDEMPHRNLETWNAY 180
           LR    GKQ+HALAVK G I+DVFVGCS FDMYSK G   +A K+FDEMP RN+ TWNAY
Sbjct: 121 LRSPLVGKQVHALAVKAGQISDVFVGCSAFDMYSKAGLTEEARKMFDEMPERNIATWNAY 180

Query: 181 ISNSVLHGRPEDSVIAFIELLRVGGKPDSITFCAFLNACSDKLGLGPGCQLHGFIIRSGY 240
           +SNSVL GR +D++ AFIE    G +P+ ITFCAFLNAC+    L  G QLHGF+++SG+
Sbjct: 181 LSNSVLEGRYDDALTAFIEFRHEGWEPNLITFCAFLNACAGASYLRLGRQLHGFVLQSGF 240

Query: 241 GQNVSVSNGLIDFYGKCGEVECSEMVFDRMGERNSVSWSSLIAAYVQNNEEEKASCLFLR 300
             +VSV+NGLIDFYGKC +V CSE++F  + + N VSW S+I +YVQN+EEEKA  +FLR
Sbjct: 241 EADVSVANGLIDFYGKCHQVGCSEIIFSGISKPNDVSWCSMIVSYVQNDEEEKACLVFLR 300

Query: 301 ARKEDIEPTDFMVSSVLCACAGLSEIEFGRSVQALAVKACVEQNIFVASALVDMYGKCGS 360
           ARKE IEPTDFMVSSVL ACAGLS +E G+SV  LAVKACV  NIFV SALVDMYGKCGS
Sbjct: 301 ARKEGIEPTDFMVSSVLSACAGLSVLEVGKSVHTLAVKACVVGNIFVGSALVDMYGKCGS 360

Query: 361 IDNAEQAFNAMPERNLVSWNALLGGYAHQGHANKAVALLEEMTSAA-GIVPSYVSLICAL 420
           I++AE+AF+ MPERNLV+WNA++GGYAHQG A+ AV L +EMT  +  + P+YV+ +C L
Sbjct: 361 IEDAERAFDEMPERNLVTWNAMIGGYAHQGQADMAVTLFDEMTCGSHRVAPNYVTFVCVL 420

Query: 421 SACSRAGDLKTGMKIFESMKERYGVEPGPEHYACLVDLLGRAGMVECAYDFIKRMPFPPT 480
           SACSRAG +  GM+IFESM+ RYG+EPG EHYAC+VDLLGRAGMVE AY FIK+MP  PT
Sbjct: 421 SACSRAGSVNVGMEIFESMRGRYGIEPGAEHYACVVDLLGRAGMVEQAYQFIKKMPIRPT 480

Query: 481 ISIWGALLGACRMHGKPELGKLAAEKLFELDPKDSGNHVVLSNMFAATGRWEEVTVVRNE 540
           +S+WGALLGA +M GK ELGK+AA+ LFELDP DSGNHV+LSNMFAA GRWEE T+VR E
Sbjct: 481 VSVWGALLGASKMFGKSELGKVAADNLFELDPLDSGNHVLLSNMFAAAGRWEEATLVRKE 540

Query: 541 MKEVGIKKGAGFSWITVDSRIHMFQAKDKSHEKDPEIQDILGKLRKEMQDAAGCIADPNY 600
           MK+VGIKKGAG SWIT  + +H+FQAKD SHE++ EIQ +L KLR EM +AAG I D ++
Sbjct: 541 MKDVGIKKGAGCSWITAGNAVHVFQAKDTSHERNSEIQAMLAKLRGEM-EAAGYIPDTSF 600

Query: 601 ALFEV 605
           ALF++
Sbjct: 601 ALFDL 604

BLAST of Cucsa.254330 vs. TrEMBL
Match: A0A067L6R6_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_02183 PE=4 SV=1)

HSP 1 Score: 814.3 bits (2102), Expect = 9.9e-233
Identity = 395/604 (65.40%), Postives = 488/604 (80.79%), Query Frame = 1

Query: 1   MPFLSQNSLASVVELAVSVRSSLLGRAAHAQILKTLKTPFPAFLYNHLVNMYAKLDHLNS 60
           MP  + NS+AS++E AVS RSSLLGRA HAQ +K+L++P P FL NHL+NMY+KLD  N 
Sbjct: 1   MPSFTPNSIASLLESAVSTRSSLLGRAVHAQAMKSLQSPLPPFLANHLINMYSKLDLPNP 60

Query: 61  AKLILELAPCRSVVTWTALIAGSVQNGCFVSALLHFSDMLSDCVRPNDFTFPCVLKASTG 120
           A L+L+L P RSVVTWTALI+GSVQNG F SALLHF +M  + ++PNDFTFPC  KAS  
Sbjct: 61  AHLVLQLTPTRSVVTWTALISGSVQNGHFSSALLHFFNMRRENIQPNDFTFPCAFKASAL 120

Query: 121 LRMDTTGKQLHALAVKEGLINDVFVGCSVFDMYSKLGFLNDAYKVFDEMPHRNLETWNAY 180
           LR    G+Q+HA+AVK G INDVFVGCS FDMYSK G  ++A K+FDE+P RN+ TWNAY
Sbjct: 121 LRSPFVGRQVHAIAVKFGQINDVFVGCSAFDMYSKTGLKHEAQKLFDELPERNVATWNAY 180

Query: 181 ISNSVLHGRPEDSVIAFIELLRVGGKPDSITFCAFLNACSDKLGLGPGCQLHGFIIRSGY 240
           ISN+VL GR E++V AFIE  RVGG+PDSITFCAFLNAC D      G QLHGF+IR+G+
Sbjct: 181 ISNAVLDGRHENAVNAFIEFRRVGGEPDSITFCAFLNACVDPQYFDLGRQLHGFVIRTGF 240

Query: 241 GQNVSVSNGLIDFYGKCGEVECSEMVFDRMGERNSVSWSSLIAAYVQNNEEEKASCLFLR 300
             ++SVSNGLIDFYGKC EV  +EMVF  M +RN+VSW SL+AA+ QN E+EKA  LFL+
Sbjct: 241 EMDMSVSNGLIDFYGKCKEVRLAEMVFGEMDKRNAVSWCSLMAAFEQNGEKEKACVLFLK 300

Query: 301 ARKEDIEPTDFMVSSVLCACAGLSEIEFGRSVQALAVKACVEQNIFVASALVDMYGKCGS 360
            RKE +EPT++MVSSV+ ACAG + +E GRSV ALA+KAC+E +IFV SALVDMYGKCGS
Sbjct: 301 GRKEGVEPTNYMVSSVISACAGFAALELGRSVHALALKACLEDDIFVGSALVDMYGKCGS 360

Query: 361 IDNAEQAFNAMPERNLVSWNALLGGYAHQGHANKAVALLEEMTSAAGIVPSYVSLICALS 420
           I++  QAF  MPERNLV+WNA++ GYAHQGHA+ A+ + EEMTS   + P+YV+++C LS
Sbjct: 361 IEDCRQAFQEMPERNLVTWNAMISGYAHQGHADMAIRVFEEMTSE--VAPNYVTMVCVLS 420

Query: 421 ACSRAGDLKTGMKIFESMKERYGVEPGPEHYACLVDLLGRAGMVECAYDFIKRMPFPPTI 480
           ACSR G ++ GM++FESM++RY +EPG EHYAC+VDLLGRAGMVE AY+F+++MP  PTI
Sbjct: 421 ACSRGGAVELGMELFESMRDRYSIEPGAEHYACIVDLLGRAGMVEHAYEFVQKMPIHPTI 480

Query: 481 SIWGALLGACRMHGKPELGKLAAEKLFELDPKDSGNHVVLSNMFAATGRWEEVTVVRNEM 540
            +WGALL ACR++GKPELGKLAA+ LFELDPKDSGNHV+LSNMFAA GRWEE T+VR EM
Sbjct: 481 EVWGALLNACRVYGKPELGKLAADNLFELDPKDSGNHVLLSNMFAAVGRWEEATLVRKEM 540

Query: 541 KEVGIKKGAGFSWITVDSRIHMFQAKDKSHEKDPEIQDILGKLRKEMQDAAGCIADPNYA 600
            +VGIKKGAG SW+T  +R+H+FQAKD SH  + EIQ +L KLR EM+ AAG + D NYA
Sbjct: 541 MDVGIKKGAGCSWLTAKNRVHVFQAKDTSHAMNYEIQAMLVKLRMEMK-AAGYVPDTNYA 600

Query: 601 LFEV 605
           L+++
Sbjct: 601 LYDL 601

BLAST of Cucsa.254330 vs. TrEMBL
Match: M5XYH8_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa002602mg PE=4 SV=1)

HSP 1 Score: 808.5 bits (2087), Expect = 5.4e-231
Identity = 397/574 (69.16%), Postives = 477/574 (83.10%), Query Frame = 1

Query: 32  ILKTLKTPFPAFLYNHLVNMYAKLDHLNSAKLILELAPCRSVVTWTALIAGSVQNGCFVS 91
           +++TL  P P+FL NHLVNMY+KLD  +SA+L+L+L P RSVVTWTALIAGSVQNG F S
Sbjct: 1   MIRTLDAPLPSFLSNHLVNMYSKLDLPDSAQLVLQLNPSRSVVTWTALIAGSVQNGHFAS 60

Query: 92  ALLHFSDMLSDCVRPNDFTFPCVLKASTGLRMDTTGKQLHALAVKEGLINDVFVGCSVFD 151
           A+LHF++ML + V+PNDFTFPC  KAS  LR+  TGKQ+HALAVK G I DVFVGCS FD
Sbjct: 61  AILHFANMLRESVQPNDFTFPCAFKASGSLRLPATGKQVHALAVKAGQICDVFVGCSAFD 120

Query: 152 MYSKLGFLNDAYKVFDEMPHRNLETWNAYISNSVLHGRPEDSVIAFIELLRVGGKPDSIT 211
           MY K G  ++A KVFDEMP RNL TWNAY+SN+VL GRP+++V  FIE LR GG+P+SIT
Sbjct: 121 MYCKTGLRDEARKVFDEMPERNLATWNAYMSNAVLDGRPQNAVYKFIEFLRAGGEPNSIT 180

Query: 212 FCAFLNACSDKLGLGPGCQLHGFIIRSGYGQNVSVSNGLIDFYGKCGEVECSEMVFDRMG 271
           FCAFLNACSD   L  G QLHGF++R G+G++VSV NGLIDFYGKC EV  S MVFD + 
Sbjct: 181 FCAFLNACSDTSNLELGRQLHGFVMRCGFGKDVSVLNGLIDFYGKCREVGSSMMVFDTID 240

Query: 272 ERNSVSWSSLIAAYVQNNEEEKASCLFLRARKEDIEPTDFMVSSVLCACAGLSEIEFGRS 331
           +RN VSW SL+AA VQN+EEE A  LFLRARKE +EPTDFMVSSVL AC+GL+ +E GRS
Sbjct: 241 KRNDVSWCSLVAACVQNDEEEMACELFLRARKEGVEPTDFMVSSVLSACSGLAWLEQGRS 300

Query: 332 VQALAVKACVEQNIFVASALVDMYGKCGSIDNAEQAFNAMPERNLVSWNALLGGYAHQGH 391
           V A+AVKACVE N+FV SALVDMYGKCGSI++A+ AFN MP RNL+SWNA++GGYAHQGH
Sbjct: 301 VHAIAVKACVEGNLFVGSALVDMYGKCGSIEDAKCAFNGMPSRNLISWNAMVGGYAHQGH 360

Query: 392 ANKAVALLEEMT-SAAGIVPSYVSLICALSACSRAGDLKTGMKIFESMKERYGVEPGPEH 451
           AN A+ L EEMT  +  + P+YV+L+C LSACSRAG ++TGM+IFESMK +YG+EPG EH
Sbjct: 361 ANMALVLFEEMTVRSHEVKPNYVTLVCVLSACSRAGAVETGMQIFESMKAKYGIEPGAEH 420

Query: 452 YACLVDLLGRAGMVECAYDFIKRMPFPPTISIWGALLGACRMHGKPELGKLAAEKLFELD 511
           YAC+VDLLGRAGMVE AY+FI +MP  PTISIWGALLGAC+M+ K ELG++AA+KLFELD
Sbjct: 421 YACVVDLLGRAGMVERAYEFITKMPIRPTISIWGALLGACKMYRKTELGRVAADKLFELD 480

Query: 512 PKDSGNHVVLSNMFAATGRWEEVTVVRNEMKEVGIKKGAGFSWITVDSRIHMFQAKDKSH 571
           PKDSGNHV+LSNMFAA GRWEE T+VR  MK+VGIKKGAG+SWI V + +H+FQAKD SH
Sbjct: 481 PKDSGNHVILSNMFAAAGRWEEATLVRKGMKDVGIKKGAGYSWIAVKNAVHVFQAKDTSH 540

Query: 572 EKDPEIQDILGKLRKEMQDAAGCIADPNYALFEV 605
           E++ EIQ +L KLR+EM + AG IAD N+ALF++
Sbjct: 541 ERNSEIQAMLTKLRREM-EKAGYIADTNFALFDL 573

BLAST of Cucsa.254330 vs. TrEMBL
Match: M1BN15_SOLTU (Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400019006 PE=4 SV=1)

HSP 1 Score: 805.1 bits (2078), Expect = 6.0e-230
Identity = 392/608 (64.47%), Postives = 487/608 (80.10%), Query Frame = 1

Query: 1   MPFLSQNSLASVVELAVSVRSSLLGRAAHAQILKTLKTPFPAFLYNHLVNMYAKLDHLNS 60
           MP LS N+L  ++E A+S +S LLGRA HA I++T+++PFP FL NHL+N Y+KLD  NS
Sbjct: 1   MPLLSANALGWLIESALSTQSLLLGRAVHAHIIRTIESPFPPFLSNHLINFYSKLDSPNS 60

Query: 61  AKLILELAP--CRSVVTWTALIAGSVQNGCFVSALLHFSDMLSDCVRPNDFTFPCVLKAS 120
           A+L+L L P   RSVVTWTALIAGSVQNG F SALLHFSDM    V+PNDFTFPC+ KAS
Sbjct: 61  AQLLLSLTPPRFRSVVTWTALIAGSVQNGHFTSALLHFSDMRRQSVQPNDFTFPCLFKAS 120

Query: 121 TGLRMDTTGKQLHALAVKEGLINDVFVGCSVFDMYSKLGFLNDAYKVFDEMPHRNLETWN 180
             L     G+QLHALA+K   INDVFVGCS FDMY K G    A K+FDEMPHRN+ TWN
Sbjct: 121 AFLHYPLMGQQLHALALKGSFINDVFVGCSAFDMYCKNGLREYAQKMFDEMPHRNIATWN 180

Query: 181 AYISNSVLHGRPEDSVIAFIELLRVGGKP-DSITFCAFLNACSDKLGLGPGCQLHGFIIR 240
           A ISNSVL GRP D+ + F+ELLR+G +P +SITFC FLNACSD L L  G QLHG++IR
Sbjct: 181 ACISNSVLDGRPYDASLKFVELLRLGEEPPNSITFCVFLNACSDGLYLKLGQQLHGYVIR 240

Query: 241 SGYGQNVSVSNGLIDFYGKCGEVECSEMVFDRMGERNSVSWSSLIAAYVQNNEEEKASCL 300
            G+G +VSV NG++DFYGKC +V+ SE+VF+ +  RN VSW +++A Y QN+  + A  L
Sbjct: 241 FGFGSDVSVLNGMVDFYGKCHQVKYSELVFNEINVRNGVSWCTMLAVYEQNDIWDNAFML 300

Query: 301 FLRARKEDIEPTDFMVSSVLCACAGLSEIEFGRSVQALAVKACVEQNIFVASALVDMYGK 360
           FL+ARKE I+PT+FM+SSVL ACAG++ +E GRS+  LAVKAC+E N+FV SALVDMYGK
Sbjct: 301 FLKARKEGIKPTEFMLSSVLSACAGMAVLELGRSIHGLAVKACIEHNVFVGSALVDMYGK 360

Query: 361 CGSIDNAEQAFNAMPERNLVSWNALLGGYAHQGHANKAVALLEEMTSAA-GIVPSYVSLI 420
           CGSIDN E +F  MPERNL++WNA++GGYAHQG A+ A++L EEMTS +  +VPSYV+ +
Sbjct: 361 CGSIDNCESSFYEMPERNLITWNAVMGGYAHQGCADMALSLFEEMTSESHNVVPSYVTFV 420

Query: 421 CALSACSRAGDLKTGMKIFESMKERYGVEPGPEHYACLVDLLGRAGMVECAYDFIKRMPF 480
           C L+ACSRAG +K GM IFESM+++YG+EPGPEHYAC+VD+LGRAG+VE AYDFIK+MP 
Sbjct: 421 CVLTACSRAGAVKIGMDIFESMQKKYGIEPGPEHYACVVDILGRAGLVERAYDFIKKMPV 480

Query: 481 PPTISIWGALLGACRMHGKPELGKLAAEKLFELDPKDSGNHVVLSNMFAATGRWEEVTVV 540
           PPT+S+WGALLGACR+HGKPELGK+AA+ LF LDP DSGNHV+LSNMFAA GRW+E  +V
Sbjct: 481 PPTVSVWGALLGACRVHGKPELGKVAADNLFRLDPLDSGNHVILSNMFAAAGRWDEANLV 540

Query: 541 RNEMKEVGIKKGAGFSWITVDSRIHMFQAKDKSHEKDPEIQDILGKLRKEMQDAAGCIAD 600
           R EMK+VGI KGAG SWI+  + IH+FQAKD +HE+ PEIQ +L KLR++M+ A G IAD
Sbjct: 541 RKEMKDVGITKGAGISWISAKNSIHIFQAKDTTHERYPEIQAMLAKLRRDMK-AEGYIAD 600

Query: 601 PNYALFEV 605
            N AL+++
Sbjct: 601 TNSALYDL 607

BLAST of Cucsa.254330 vs. TAIR10
Match: AT4G14850.1 (AT4G14850.1 Pentatricopeptide repeat (PPR) superfamily protein)

HSP 1 Score: 760.4 bits (1962), Expect = 8.6e-220
Identity = 372/605 (61.49%), Postives = 469/605 (77.52%), Query Frame = 1

Query: 1   MPFLSQNSLASVVELAVSVRSSLLGRAAHAQILKTLKTPFPAFLYNHLVNMYAKLDHLNS 60
           M  LS ++L  +++ A+S  S  LGR  HA+I+KTL +P P FL N+L+NMY+KLDH  S
Sbjct: 1   MSLLSADALGLLLKNAISASSMRLGRVVHARIVKTLDSPPPPFLANYLINMYSKLDHPES 60

Query: 61  AKLILELAPCRSVVTWTALIAGSVQNGCFVSALLHFSDMLSDCVRPNDFTFPCVLKASTG 120
           A+L+L L P R+VV+WT+LI+G  QNG F +AL+ F +M  + V PNDFTFPC  KA   
Sbjct: 61  ARLVLRLTPARNVVSWTSLISGLAQNGHFSTALVEFFEMRREGVVPNDFTFPCAFKAVAS 120

Query: 121 LRMDTTGKQLHALAVKEGLINDVFVGCSVFDMYSKLGFLNDAYKVFDEMPHRNLETWNAY 180
           LR+  TGKQ+HALAVK G I DVFVGCS FDMY K    +DA K+FDE+P RNLETWNA+
Sbjct: 121 LRLPVTGKQIHALAVKCGRILDVFVGCSAFDMYCKTRLRDDARKLFDEIPERNLETWNAF 180

Query: 181 ISNSVLHGRPEDSVIAFIELLRVGGKPDSITFCAFLNACSDKLGLGPGCQLHGFIIRSGY 240
           ISNSV  GRP +++ AFIE  R+ G P+SITFCAFLNACSD L L  G QLHG ++RSG+
Sbjct: 181 ISNSVTDGRPREAIEAFIEFRRIDGHPNSITFCAFLNACSDWLHLNLGMQLHGLVLRSGF 240

Query: 241 GQNVSVSNGLIDFYGKCGEVECSEMVFDRMGERNSVSWSSLIAAYVQNNEEEKASCLFLR 300
             +VSV NGLIDFYGKC ++  SE++F  MG +N+VSW SL+AAYVQN+E+EKAS L+LR
Sbjct: 241 DTDVSVCNGLIDFYGKCKQIRSSEIIFTEMGTKNAVSWCSLVAAYVQNHEDEKASVLYLR 300

Query: 301 ARKEDIEPTDFMVSSVLCACAGLSEIEFGRSVQALAVKACVEQNIFVASALVDMYGKCGS 360
           +RK+ +E +DFM+SSVL ACAG++ +E GRS+ A AVKACVE+ IFV SALVDMYGKCG 
Sbjct: 301 SRKDIVETSDFMISSVLSACAGMAGLELGRSIHAHAVKACVERTIFVGSALVDMYGKCGC 360

Query: 361 IDNAEQAFNAMPERNLVSWNALLGGYAHQGHANKAVALLEEMT-SAAGIVPSYVSLICAL 420
           I+++EQAF+ MPE+NLV+ N+L+GGYAHQG  + A+AL EEM     G  P+Y++ +  L
Sbjct: 361 IEDSEQAFDEMPEKNLVTRNSLIGGYAHQGQVDMALALFEEMAPRGCGPTPNYMTFVSLL 420

Query: 421 SACSRAGDLKTGMKIFESMKERYGVEPGPEHYACLVDLLGRAGMVECAYDFIKRMPFPPT 480
           SACSRAG ++ GMKIF+SM+  YG+EPG EHY+C+VD+LGRAGMVE AY+FIK+MP  PT
Sbjct: 421 SACSRAGAVENGMKIFDSMRSTYGIEPGAEHYSCIVDMLGRAGMVERAYEFIKKMPIQPT 480

Query: 481 ISIWGALLGACRMHGKPELGKLAAEKLFELDPKDSGNHVVLSNMFAATGRWEEVTVVRNE 540
           IS+WGAL  ACRMHGKP+LG LAAE LF+LDPKDSGNHV+LSN FAA GRW E   VR E
Sbjct: 481 ISVWGALQNACRMHGKPQLGLLAAENLFKLDPKDSGNHVLLSNTFAAAGRWAEANTVREE 540

Query: 541 MKEVGIKKGAGFSWITVDSRIHMFQAKDKSHEKDPEIQDILGKLRKEMQDAAGCIADPNY 600
           +K VGIKKGAG+SWITV +++H FQAKD+SH  + EIQ  L KLR EM +AAG   D   
Sbjct: 541 LKGVGIKKGAGYSWITVKNQVHAFQAKDRSHILNKEIQTTLAKLRNEM-EAAGYKPDLKL 600

Query: 601 ALFEV 605
           +L+++
Sbjct: 601 SLYDL 604

BLAST of Cucsa.254330 vs. TAIR10
Match: AT5G09950.1 (AT5G09950.1 Tetratricopeptide repeat (TPR)-like superfamily protein)

HSP 1 Score: 418.7 bits (1075), Expect = 6.1e-117
Identity = 226/585 (38.63%), Postives = 342/585 (58.46%), Query Frame = 1

Query: 25  GRAAHAQILKTLKTPFPAFLYNHLVNMYAKLDHLNSAKLILELAPCRSVVTWTALIAGSV 84
           GR  H  ++ T    F   + N LVNMYAK   +  A+ +      +  V+W ++I G  
Sbjct: 332 GREVHGHVITTGLVDFMVGIGNGLVNMYAKCGSIADARRVFYFMTDKDSVSWNSMITGLD 391

Query: 85  QNGCFVSALLHFSDMLSDCVRPNDFTFPCVLKASTGLRMDTTGKQLHALAVKEGLINDVF 144
           QNGCF+ A+  +  M    + P  FT    L +   L+    G+Q+H  ++K G+  +V 
Sbjct: 392 QNGCFIEAVERYKSMRRHDILPGSFTLISSLSSCASLKWAKLGQQIHGESLKLGIDLNVS 451

Query: 145 VGCSVFDMYSKLGFLNDAYKVFDEMPHRNLETWNAYISNSVLHGRP-EDSVIAFIELLRV 204
           V  ++  +Y++ G+LN+  K+F  MP  +  +WN+ I       R   ++V+ F+   R 
Sbjct: 452 VSNALMTLYAETGYLNECRKIFSSMPEHDQVSWNSIIGALARSERSLPEAVVCFLNAQRA 511

Query: 205 GGKPDSITFCAFLNACSDKLGLGP-GCQLHGFIIRSGYGQNVSVSNGLIDFYGKCGEVEC 264
           G K + ITF + L+A S  L  G  G Q+HG  +++      +  N LI  YGKCGE++ 
Sbjct: 512 GQKLNRITFSSVLSAVSS-LSFGELGKQIHGLALKNNIADEATTENALIACYGKCGEMDG 571

Query: 265 SEMVFDRMGER-NSVSWSSLIAAYVQNNEEEKASCLFLRARKEDIEPTDFMVSSVLCACA 324
            E +F RM ER ++V+W+S+I+ Y+ N    KA  L     +       FM ++VL A A
Sbjct: 572 CEKIFSRMAERRDNVTWNSMISGYIHNELLAKALDLVWFMLQTGQRLDSFMYATVLSAFA 631

Query: 325 GLSEIEFGRSVQALAVKACVEQNIFVASALVDMYGKCGSIDNAEQAFNAMPERNLVSWNA 384
            ++ +E G  V A +V+AC+E ++ V SALVDMY KCG +D A + FN MP RN  SWN+
Sbjct: 632 SVATLERGMEVHACSVRACLESDVVVGSALVDMYSKCGRLDYALRFFNTMPVRNSYSWNS 691

Query: 385 LLGGYAHQGHANKAVALLEEMTSAAGIVPSYVSLICALSACSRAGDLKTGMKIFESMKER 444
           ++ GYA  G   +A+ L E M       P +V+ +  LSACS AG L+ G K FESM + 
Sbjct: 692 MISGYARHGQGEEALKLFETMKLDGQTPPDHVTFVGVLSACSHAGLLEEGFKHFESMSDS 751

Query: 445 YGVEPGPEHYACLVDLLGRAGMVECAYDFIKRMPFPPTISIWGALLGA-CRMHG-KPELG 504
           YG+ P  EH++C+ D+LGRAG ++   DFI++MP  P + IW  +LGA CR +G K ELG
Sbjct: 752 YGLAPRIEHFSCMADVLGRAGELDKLEDFIEKMPMKPNVLIWRTVLGACCRANGRKAELG 811

Query: 505 KLAAEKLFELDPKDSGNHVVLSNMFAATGRWEEVTVVRNEMKEVGIKKGAGFSWITVDSR 564
           K AAE LF+L+P+++ N+V+L NM+AA GRWE++   R +MK+  +KK AG+SW+T+   
Sbjct: 812 KKAAEMLFQLEPENAVNYVLLGNMYAAGGRWEDLVKARKKMKDADVKKEAGYSWVTMKDG 871

Query: 565 IHMFQAKDKSHEKDPEIQDILGKLRKEMQDAAGCIADPNYALFEV 605
           +HMF A DKSH     I   L +L ++M+D AG +    +AL+++
Sbjct: 872 VHMFVAGDKSHPDADVIYKKLKELNRKMRD-AGYVPQTGFALYDL 914

HSP 2 Score: 178.7 bits (452), Expect = 1.1e-44
Identity = 145/497 (29.18%), Postives = 240/497 (48.29%), Query Frame = 1

Query: 43  FLYNHLVNMYAKLDHLNSAKLILELAPCRSVVTWTALIAGSVQNGCFVSALLHFSDMLSD 102
           F+ + LV+ +AK   L+ A+ +      R+ VT   L+ G V+      A   F DM S 
Sbjct: 244 FVGSGLVSAFAKSGSLSYARKVFNQMETRNAVTLNGLMVGLVRQKWGEEATKLFMDMNSM 303

Query: 103 C-VRPNDF-----TFP-CVLKASTGLRMDTTGKQLHALAVKEGLIND-VFVGCSVFDMYS 162
             V P  +     +FP   L    GL+    G+++H   +  GL++  V +G  + +MY+
Sbjct: 304 IDVSPESYVILLSSFPEYSLAEEVGLKK---GREVHGHVITTGLVDFMVGIGNGLVNMYA 363

Query: 163 KLGFLNDAYKVFDEMPHRNLETWNAYISNSVLHGRPEDSVIAFIELLRVGGKPDSITFCA 222
           K G + DA +VF  M  ++  +WN+ I+    +G   ++V  +  + R    P S T  +
Sbjct: 364 KCGSIADARRVFYFMTDKDSVSWNSMITGLDQNGCFIEAVERYKSMRRHDILPGSFTLIS 423

Query: 223 FLNACSDKLGLGPGCQLHGFIIRSGYGQNVSVSNGLIDFYGKCGEVECSEMVFDRMGERN 282
            L++C+       G Q+HG  ++ G   NVSVSN L+  Y + G +     +F  M E +
Sbjct: 424 SLSSCASLKWAKLGQQIHGESLKLGIDLNVSVSNALMTLYAETGYLNECRKIFSSMPEHD 483

Query: 283 SVSWSSLIAAYVQNNEE-EKASCLFLRARKEDIEPTDFMVSSVLCACAGLSEIEFGRSVQ 342
            VSW+S+I A  ++     +A   FL A++   +      SSVL A + LS  E G+ + 
Sbjct: 484 QVSWNSIIGALARSERSLPEAVVCFLNAQRAGQKLNRITFSSVLSAVSSLSFGELGKQIH 543

Query: 343 ALAVKACVEQNIFVASALVDMYGKCGSIDNAEQAFNAMPE-RNLVSWNALLGGYAHQGHA 402
            LA+K  +       +AL+  YGKCG +D  E+ F+ M E R+ V+WN+++ GY H    
Sbjct: 544 GLALKNNIADEATTENALIACYGKCGEMDGCEKIFSRMAERRDNVTWNSMISGYIHNELL 603

Query: 403 NKAVALLEEMTSAAGIVPSYVSLICALSACSRAGDLKTGMKIFESMKERYGVEPGPEHYA 462
            KA+ L+  M      + S++     LSA +    L+ GM++  +   R  +E      +
Sbjct: 604 AKALDLVWFMLQTGQRLDSFM-YATVLSAFASVATLERGMEV-HACSVRACLESDVVVGS 663

Query: 463 CLVDLLGRAGMVECAYDFIKRMPFPPTISIWGALLGACRMHGKPELGKLAAEKLFE---L 522
            LVD+  + G ++ A  F   MP   + S W +++     HG+ E     A KLFE   L
Sbjct: 664 ALVDMYSKCGRLDYALRFFNTMPVRNSYS-WNSMISGYARHGQGE----EALKLFETMKL 723

Query: 523 DPKDSGNHVVLSNMFAA 527
           D +   +HV    + +A
Sbjct: 724 DGQTPPDHVTFVGVLSA 730

HSP 3 Score: 150.2 bits (378), Expect = 4.1e-36
Identity = 132/493 (26.77%), Postives = 232/493 (47.06%), Query Frame = 1

Query: 11  SVVELAVSVRSSLLGRAAHAQILKTLKTPFPAFLYNHLVNMYAKLDHLNSAKLILELAPC 70
           S V+  V  R +   R  H+++ K  +     +L N+L+N Y +     SA+ + +  P 
Sbjct: 8   SFVQSCVGHRGA--ARFFHSRLYKN-RLDKDVYLCNNLINAYLETGDSVSARKVFDEMPL 67

Query: 71  RSVVTWTALIAGSVQNGCFVSALLHFSDMLSDCVRPNDFTFPCVLKA-----STGLRMDT 130
           R+ V+W  +++G  +NG    AL+   DM+ + +  N + F  VL+A     S G+    
Sbjct: 68  RNCVSWACIVSGYSRNGEHKEALVFLRDMVKEGIFSNQYAFVSVLRACQEIGSVGILF-- 127

Query: 131 TGKQLHALAVKEGLINDVFVGCSVFDMYSK-LGFLNDAYKVFDEMPHRNLETWNAYISNS 190
            G+Q+H L  K     D  V   +  MY K +G +  A   F ++  +N  +WN+ IS  
Sbjct: 128 -GRQIHGLMFKLSYAVDAVVSNVLISMYWKCIGSVGYALCAFGDIEVKNSVSWNSIISVY 187

Query: 191 VLHGRPEDSVIAFIELLRVGGKPDSITFCAFLN-ACS-DKLGLGPGCQLHGFIIRSGYGQ 250
              G    +   F  +   G +P   TF + +  ACS  +  +    Q+   I +SG   
Sbjct: 188 SQAGDQRSAFRIFSSMQYDGSRPTEYTFGSLVTTACSLTEPDVRLLEQIMCTIQKSGLLT 247

Query: 251 NVSVSNGLIDFYGKCGEVECSEMVFDRMGERNSVSWSSLIAAYVQNNEEEKASCLFLRAR 310
           ++ V +GL+  + K G +  +  VF++M  RN+V+ + L+   V+    E+A+ LF+   
Sbjct: 248 DLFVGSGLVSAFAKSGSLSYARKVFNQMETRNAVTLNGLMVGLVRQKWGEEATKLFMDMN 307

Query: 311 KE-DIEPTDFMVSSVLCACAGLSE---IEFGRSVQALAVKA-CVEQNIFVASALVDMYGK 370
              D+ P  +++         L+E   ++ GR V    +    V+  + + + LV+MY K
Sbjct: 308 SMIDVSPESYVILLSSFPEYSLAEEVGLKKGREVHGHVITTGLVDFMVGIGNGLVNMYAK 367

Query: 371 CGSIDNAEQAFNAMPERNLVSWNALLGGYAHQGHANKAVALLEEMTSAAGIVPSYVSLIC 430
           CGSI +A + F  M +++ VSWN+++ G    G   +AV   + M     I+P   +LI 
Sbjct: 368 CGSIADARRVFYFMTDKDSVSWNSMITGLDQNGCFIEAVERYKSM-RRHDILPGSFTLIS 427

Query: 431 ALSACSRAGDLKTGMKIF-ESMKERYGVEPGPEHYACLVDLLGRAGMVECAYDFIKRMPF 490
           +LS+C+     K G +I  ES+K   G++        L+ L    G +         MP 
Sbjct: 428 SLSSCASLKWAKLGQQIHGESLK--LGIDLNVSVSNALMTLYAETGYLNECRKIFSSMPE 487

HSP 4 Score: 97.4 bits (241), Expect = 3.1e-20
Identity = 82/323 (25.39%), Postives = 142/323 (43.96%), Query Frame = 1

Query: 232 HGFIIRSGYGQNVSVSNGLIDFYGKCGEVECSEMVFDRMGERNSVSWSSLIAAYVQNNEE 291
           H  + ++   ++V + N LI+ Y + G+   +  VFD M  RN VSW+ +++ Y +N E 
Sbjct: 24  HSRLYKNRLDKDVYLCNNLINAYLETGDSVSARKVFDEMPLRNCVSWACIVSGYSRNGEH 83

Query: 292 EKASCLFLRARKEDIEPTDFMVSSVLCAC--AGLSEIEFGRSVQALAVKACVEQNIFVAS 351
           ++A        KE I    +   SVL AC   G   I FGR +  L  K     +  V++
Sbjct: 84  KEALVFLRDMVKEGIFSNQYAFVSVLRACQEIGSVGILFGRQIHGLMFKLSYAVDAVVSN 143

Query: 352 ALVDMYGKC-GSIDNAEQAFNAMPERNLVSWNALLGGYAHQGHANKAVALLEEMTSAAGI 411
            L+ MY KC GS+  A  AF  +  +N VSWN+++  Y+  G    A  +   M      
Sbjct: 144 VLISMYWKCIGSVGYALCAFGDIEVKNSVSWNSIISVYSQAGDQRSAFRIFSSMQYDGSR 203

Query: 412 VPSYVSLICALSACSRAGDLKTGMKIFESMK---ERYGVEPGPEHYACLVDLLGRAGMVE 471
              Y       +ACS     +  +++ E +    ++ G+       + LV    ++G + 
Sbjct: 204 PTEYTFGSLVTTACSLT---EPDVRLLEQIMCTIQKSGLLTDLFVGSGLVSAFAKSGSLS 263

Query: 472 CAYDFIKRMPFPPTISIWGALLGACRMHGKPELGKLAAEKLFELDPKDSGNHVVLSNMFA 531
            A     +M     +++ G ++G  R     E  KL  +    +D       ++LS+   
Sbjct: 264 YARKVFNQMETRNAVTLNGLMVGLVRQKWGEEATKLFMDMNSMIDVSPESYVILLSS--- 323

Query: 532 ATGRWEEVTVVRNEMKEVGIKKG 549
               + E ++     +EVG+KKG
Sbjct: 324 ----FPEYSLA----EEVGLKKG 332

BLAST of Cucsa.254330 vs. TAIR10
Match: AT1G11290.1 (AT1G11290.1 Pentatricopeptide repeat (PPR) superfamily protein)

HSP 1 Score: 383.6 bits (984), Expect = 2.2e-106
Identity = 205/578 (35.47%), Postives = 328/578 (56.75%), Query Frame = 1

Query: 24  LGRAAHAQILKTLKTPFPAFLYNHLVNMYAKLDHLNSAKLILELAPCRSVVTWTALIAGS 83
           +G+  H  ++K+       F    L NMYAK   +N A+ + +  P R +V+W  ++AG 
Sbjct: 153 VGKEIHGLLVKS-GFSLDLFAMTGLENMYAKCRQVNEARKVFDRMPERDLVSWNTIVAGY 212

Query: 84  VQNGCFVSALLHFSDMLSDCVRPNDFTFPCVLKASTGLRMDTTGKQLHALAVKEGLINDV 143
            QNG    AL     M  + ++P+  T   VL A + LR+ + GK++H  A++ G  + V
Sbjct: 213 SQNGMARMALEMVKSMCEENLKPSFITIVSVLPAVSALRLISVGKEIHGYAMRSGFDSLV 272

Query: 144 FVGCSVFDMYSKLGFLNDAYKVFDEMPHRNLETWNAYISNSVLHGRPEDSVIAFIELLRV 203
            +  ++ DMY+K G L  A ++FD M  RN+ +WN+ I   V +  P+++++ F ++L  
Sbjct: 273 NISTALVDMYAKCGSLETARQLFDGMLERNVVSWNSMIDAYVQNENPKEAMLIFQKMLDE 332

Query: 204 GGKPDSITFCAFLNACSDKLGLGPGCQLHGFIIRSGYGQNVSVSNGLIDFYGKCGEVECS 263
           G KP  ++    L+AC+D   L  G  +H   +  G  +NVSV N LI  Y KC EV+ +
Sbjct: 333 GVKPTDVSVMGALHACADLGDLERGRFIHKLSVELGLDRNVSVVNSLISMYCKCKEVDTA 392

Query: 264 EMVFDRMGERNSVSWSSLIAAYVQNNEEEKASCLFLRARKEDIEPTDFMVSSVLCACAGL 323
             +F ++  R  VSW+++I  + QN     A   F + R   ++P  F   SV+ A A L
Sbjct: 393 ASMFGKLQSRTLVSWNAMILGFAQNGRPIDALNYFSQMRSRTVKPDTFTYVSVITAIAEL 452

Query: 324 SEIEFGRSVQALAVKACVEQNIFVASALVDMYGKCGSIDNAEQAFNAMPERNLVSWNALL 383
           S     + +  + +++C+++N+FV +ALVDMY KCG+I  A   F+ M ER++ +WNA++
Sbjct: 453 SITHHAKWIHGVVMRSCLDKNVFVTTALVDMYAKCGAIMIARLIFDMMSERHVTTWNAMI 512

Query: 384 GGYAHQGHANKAVALLEEMTSAAGIVPSYVSLICALSACSRAGDLKTGMKIFESMKERYG 443
            GY   G    A+ L EEM     I P+ V+ +  +SACS +G ++ G+K F  MKE Y 
Sbjct: 513 DGYGTHGFGKAALELFEEMQKGT-IKPNGVTFLSVISACSHSGLVEAGLKCFYMMKENYS 572

Query: 444 VEPGPEHYACLVDLLGRAGMVECAYDFIKRMPFPPTISIWGALLGACRMHGKPELGKLAA 503
           +E   +HY  +VDLLGRAG +  A+DFI +MP  P ++++GA+LGAC++H      + AA
Sbjct: 573 IELSMDHYGAMVDLLGRAGRLNEAWDFIMQMPVKPAVNVYGAMLGACQIHKNVNFAEKAA 632

Query: 504 EKLFELDPKDSGNHVVLSNMFAATGRWEEVTVVRNEMKEVGIKKGAGFSWITVDSRIHMF 563
           E+LFEL+P D G HV+L+N++ A   WE+V  VR  M   G++K  G S + + + +H F
Sbjct: 633 ERLFELNPDDGGYHVLLANIYRAASMWEKVGQVRVSMLRQGLRKTPGCSMVEIKNEVHSF 692

Query: 564 QAKDKSHEKDPEIQDILGKLRKEMQDAAGCIADPNYAL 602
            +   +H    +I   L KL   +++ AG + D N  L
Sbjct: 693 FSGSTAHPDSKKIYAFLEKLICHIKE-AGYVPDTNLVL 727

HSP 2 Score: 226.1 bits (575), Expect = 5.8e-59
Identity = 147/469 (31.34%), Postives = 233/469 (49.68%), Query Frame = 1

Query: 43  FLYNHLVNMYAKLDHLNSAKLILELAPCRSVVTWTALIAGSVQNGCFVSALLHFSDMLSD 102
           F    LV+++ +   ++ A  + E    +  V +  ++ G  +      AL  F  M  D
Sbjct: 70  FFQTKLVSLFCRYGSVDEAARVFEPIDSKLNVLYHTMLKGFAKVSDLDKALQFFVRMRYD 129

Query: 103 CVRPNDFTFPCVLKASTGLRMDTTGKQLHALAVKEGLINDVFVGCSVFDMYSKLGFLNDA 162
            V P  + F  +LK          GK++H L VK G   D+F    + +MY+K   +N+A
Sbjct: 130 DVEPVVYNFTYLLKVCGDEAELRVGKEIHGLLVKSGFSLDLFAMTGLENMYAKCRQVNEA 189

Query: 163 YKVFDEMPHRNLETWNAYISNSVLHGRPEDSVIAFIELLRVGGKPDSITFCAFLNACSDK 222
            KVFD MP R+L +WN  ++    +G    ++     +     KP  IT  + L A S  
Sbjct: 190 RKVFDRMPERDLVSWNTIVAGYSQNGMARMALEMVKSMCEENLKPSFITIVSVLPAVSAL 249

Query: 223 LGLGPGCQLHGFIIRSGYGQNVSVSNGLIDFYGKCGEVECSEMVFDRMGERNSVSWSSLI 282
             +  G ++HG+ +RSG+   V++S  L+D Y KCG +E +  +FD M ERN VSW+S+I
Sbjct: 250 RLISVGKEIHGYAMRSGFDSLVNISTALVDMYAKCGSLETARQLFDGMLERNVVSWNSMI 309

Query: 283 AAYVQNNEEEKASCLFLRARKEDIEPTDFMVSSVLCACAGLSEIEFGRSVQALAVKACVE 342
            AYVQN   ++A  +F +   E ++PTD  V   L ACA L ++E GR +  L+V+  ++
Sbjct: 310 DAYVQNENPKEAMLIFQKMLDEGVKPTDVSVMGALHACADLGDLERGRFIHKLSVELGLD 369

Query: 343 QNIFVASALVDMYGKCGSIDNAEQAFNAMPERNLVSWNALLGGYAHQGHANKAVALLEEM 402
           +N+ V ++L+ MY KC  +D A   F  +  R LVSWNA++ G+A  G    A+    +M
Sbjct: 370 RNVSVVNSLISMYCKCKEVDTAASMFGKLQSRTLVSWNAMILGFAQNGRPIDALNYFSQM 429

Query: 403 TSAAGIVP---SYVSLICALSACSRAGDLKTGMKIFESMKERYGVEPGPEHYACLVDLLG 462
            S   + P   +YVS+I A++  S    +    K    +  R  ++        LVD+  
Sbjct: 430 RSRT-VKPDTFTYVSVITAIAELS----ITHHAKWIHGVVMRSCLDKNVFVTTALVDMYA 489

Query: 463 RAGMVECAYDFIKRMPFPPTISIWGALLGACRMHGKPELGKLAAEKLFE 509
           + G +  A   I  M     ++ W A++     HG    GK A E LFE
Sbjct: 490 KCGAIMIA-RLIFDMMSERHVTTWNAMIDGYGTHG---FGKAALE-LFE 528

BLAST of Cucsa.254330 vs. TAIR10
Match: AT3G53360.1 (AT3G53360.1 Tetratricopeptide repeat (TPR)-like superfamily protein)

HSP 1 Score: 372.5 bits (955), Expect = 5.0e-103
Identity = 195/586 (33.28%), Postives = 332/586 (56.66%), Query Frame = 1

Query: 6   QNSLASVVELAVSVRSSLLGRAAHAQILKTLKTPFPAFLYNHLVNMYAKLDHLNSAKLIL 65
           Q +  S+++   S     LG+  HAQ++K L++       N L+ MY + + ++ A  + 
Sbjct: 168 QFAFGSIIKACASSSDVGLGKQLHAQVIK-LESSSHLIAQNALIAMYVRFNQMSDASRVF 227

Query: 66  ELAPCRSVVTWTALIAGSVQNGCFVSALLHFSDMLSDCV-RPNDFTFPCVLKASTGLRMD 125
              P + +++W+++IAG  Q G    AL H  +MLS  V  PN++ F   LKA + L   
Sbjct: 228 YGIPMKDLISWSSIIAGFSQLGFEFEALSHLKEMLSFGVFHPNEYIFGSSLKACSSLLRP 287

Query: 126 TTGKQLHALAVKEGLINDVFVGCSVFDMYSKLGFLNDAYKVFDEMPHRNLETWNAYISNS 185
             G Q+H L +K  L  +   GCS+ DMY++ GFLN A +VFD++   +  +WN  I+  
Sbjct: 288 DYGSQIHGLCIKSELAGNAIAGCSLCDMYARCGFLNSARRVFDQIERPDTASWNVIIAGL 347

Query: 186 VLHGRPEDSVIAFIELLRVGGKPDSITFCAFLNACSDKLGLGPGCQLHGFIIRSGYGQNV 245
             +G  +++V  F ++   G  PD+I+  + L A +  + L  G Q+H +II+ G+  ++
Sbjct: 348 ANNGYADEAVSVFSQMRSSGFIPDAISLRSLLCAQTKPMALSQGMQIHSYIIKWGFLADL 407

Query: 246 SVSNGLIDFYGKCGEVECSEMVF-DRMGERNSVSWSSLIAAYVQNNEEEKASCLFLRARK 305
           +V N L+  Y  C ++ C   +F D     +SVSW++++ A +Q+ +  +   LF     
Sbjct: 408 TVCNSLLTMYTFCSDLYCCFNLFEDFRNNADSVSWNTILTACLQHEQPVEMLRLFKLMLV 467

Query: 306 EDIEPTDFMVSSVLCACAGLSEIEFGRSVQALAVKACVEQNIFVASALVDMYGKCGSIDN 365
            + EP    + ++L  C  +S ++ G  V   ++K  +    F+ + L+DMY KCGS+  
Sbjct: 468 SECEPDHITMGNLLRGCVEISSLKLGSQVHCYSLKTGLAPEQFIKNGLIDMYAKCGSLGQ 527

Query: 366 AEQAFNAMPERNLVSWNALLGGYAHQGHANKAVALLEEMTSAAGIVPSYVSLICALSACS 425
           A + F++M  R++VSW+ L+ GYA  G   +A+ L +EM SA GI P++V+ +  L+ACS
Sbjct: 528 ARRIFDSMDNRDVVSWSTLIVGYAQSGFGEEALILFKEMKSA-GIEPNHVTFVGVLTACS 587

Query: 426 RAGDLKTGMKIFESMKERYGVEPGPEHYACLVDLLGRAGMVECAYDFIKRMPFPPTISIW 485
             G ++ G+K++ +M+  +G+ P  EH +C+VDLL RAG +  A  FI  M   P + +W
Sbjct: 588 HVGLVEEGLKLYATMQTEHGISPTKEHCSCVVDLLARAGRLNEAERFIDEMKLEPDVVVW 647

Query: 486 GALLGACRMHGKPELGKLAAEKLFELDPKDSGNHVVLSNMFAATGRWEEVTVVRNEMKEV 545
             LL AC+  G   L + AAE + ++DP +S  HV+L +M A++G WE   ++R+ MK+ 
Sbjct: 648 KTLLSACKTQGNVHLAQKAAENILKIDPFNSTAHVLLCSMHASSGNWENAALLRSSMKKH 707

Query: 546 GIKKGAGFSWITVDSRIHMFQAKDKSHEKDPEIQDILGKLRKEMQD 590
            +KK  G SWI ++ +IH+F A+D  H +  +I  +L  +  +M D
Sbjct: 708 DVKKIPGQSWIEIEDKIHIFFAEDIFHPERDDIYTVLHNIWSQMLD 751

HSP 2 Score: 161.4 bits (407), Expect = 1.8e-39
Identity = 108/417 (25.90%), Postives = 202/417 (48.44%), Query Frame = 1

Query: 110 TFPCVLKASTGLRMDTTGKQLHALAVKEGLINDVFVGCSVFDMYSKLGFLNDAYKVFDEM 169
           T+  ++ A +  R    G+++H   +      D  +   +  MY K G L DA +VFD M
Sbjct: 69  TYISLICACSSSRSLAQGRKIHDHILNSNCKYDTILNNHILSMYGKCGSLRDAREVFDFM 128

Query: 170 PHRNLETWNAYISNSVLHGRPEDSVIAFIELLRVGGKPDSITFCAFLNACSDKLGLGPGC 229
           P RNL ++ + I+    +G+  +++  ++++L+    PD   F + + AC+    +G G 
Sbjct: 129 PERNLVSYTSVITGYSQNGQGAEAIRLYLKMLQEDLVPDQFAFGSIIKACASSSDVGLGK 188

Query: 230 QLHGFIIRSGYGQNVSVSNGLIDFYGKCGEVECSEMVFDRMGERNSVSWSSLIAAYVQ-N 289
           QLH  +I+     ++   N LI  Y +  ++  +  VF  +  ++ +SWSS+IA + Q  
Sbjct: 189 QLHAQVIKLESSSHLIAQNALIAMYVRFNQMSDASRVFYGIPMKDLISWSSIIAGFSQLG 248

Query: 290 NEEEKASCLFLRARKEDIEPTDFMVSSVLCACAGLSEIEFGRSVQALAVKACVEQNIFVA 349
            E E  S L          P +++  S L AC+ L   ++G  +  L +K+ +  N    
Sbjct: 249 FEFEALSHLKEMLSFGVFHPNEYIFGSSLKACSSLLRPDYGSQIHGLCIKSELAGNAIAG 308

Query: 350 SALVDMYGKCGSIDNAEQAFNAMPERNLVSWNALLGGYAHQGHANKAVALLEEMTSAAGI 409
            +L DMY +CG +++A + F+ +   +  SWN ++ G A+ G+A++AV++  +M S +G 
Sbjct: 309 CSLCDMYARCGFLNSARRVFDQIERPDTASWNVIIAGLANNGYADEAVSVFSQMRS-SGF 368

Query: 410 VPSYVSLICALSACSRAGDLKTGMKIFESMKERYGVEPGPEHYACLVDLLGRAGMVECAY 469
           +P  +SL   L A ++   L  GM+I  S   ++G          L+ +      + C +
Sbjct: 369 IPDAISLRSLLCAQTKPMALSQGMQI-HSYIIKWGFLADLTVCNSLLTMYTFCSDLYCCF 428

Query: 470 DFIKRMPFPPTISIWGALLGACRMHGKP-ELGKLAAEKLF-ELDPKDSGNHVVLSNM 524
           +  +          W  +L AC  H +P E+ +L    L  E +P    +H+ + N+
Sbjct: 429 NLFEDFRNNADSVSWNTILTACLQHEQPVEMLRLFKLMLVSECEP----DHITMGNL 479

HSP 3 Score: 160.6 bits (405), Expect = 3.0e-39
Identity = 121/479 (25.26%), Postives = 213/479 (44.47%), Query Frame = 1

Query: 11  SVVELAVSVRSSLLGRAAHAQILKTLKTPFPAFLYNHLVNMYAKLDHLNSAKLILELAPC 70
           S++    S RS   GR  H  IL +    +   L NH+++MY K   L  A+ + +  P 
Sbjct: 72  SLICACSSSRSLAQGRKIHDHILNS-NCKYDTILNNHILSMYGKCGSLRDAREVFDFMPE 131

Query: 71  RSVVTWTALIAGSVQNGCFVSALLHFSDMLSDCVRPNDFTFPCVLKASTGLRMDTTGKQL 130
           R++V++T++I G  QNG    A+  +  ML + + P+ F F  ++KA         GKQL
Sbjct: 132 RNLVSYTSVITGYSQNGQGAEAIRLYLKMLQEDLVPDQFAFGSIIKACASSSDVGLGKQL 191

Query: 131 HALAVKEGLINDVFVGCSVFDMYSKLGFLNDAYKVFDEMPHRNLETWNAYISNSVLHGRP 190
           HA  +K    + +    ++  MY +   ++DA +VF  +P ++L +W++ I+     G  
Sbjct: 192 HAQVIKLESSSHLIAQNALIAMYVRFNQMSDASRVFYGIPMKDLISWSSIIAGFSQLGFE 251

Query: 191 EDSVIAFIELLRVG-GKPDSITFCAFLNACSDKLGLGPGCQLHGFIIRSGYGQNVSVSNG 250
            +++    E+L  G   P+   F + L ACS  L    G Q+HG  I+S    N      
Sbjct: 252 FEALSHLKEMLSFGVFHPNEYIFGSSLKACSSLLRPDYGSQIHGLCIKSELAGNAIAGCS 311

Query: 251 LIDFYGKCGEVECSEMVFDRMGERNSVSWSSLIAAYVQNNEEEKASCLFLRARKEDIEPT 310
           L D Y +CG +  +  VFD++   ++ SW+ +IA    N   ++A  +F + R     P 
Sbjct: 312 LCDMYARCGFLNSARRVFDQIERPDTASWNVIIAGLANNGYADEAVSVFSQMRSSGFIPD 371

Query: 311 DFMVSSVLCACAGLSEIEFGRSVQALAVKACVEQNIFVASALVDMYGKCGSIDNAEQAFN 370
              + S+LCA      +  G  + +  +K     ++ V ++L+ MY  C  +      F 
Sbjct: 372 AISLRSLLCAQTKPMALSQGMQIHSYIIKWGFLADLTVCNSLLTMYTFCSDLYCCFNLFE 431

Query: 371 AMPER-NLVSWNALLGGYAHQGHANKAVALLEEMTSAAGIVPSYVSLICALSACSRAGDL 430
                 + VSWN +L          + + L + M   +   P ++++   L  C     L
Sbjct: 432 DFRNNADSVSWNTILTACLQHEQPVEMLRLFKLML-VSECEPDHITMGNLLRGCVEISSL 491

Query: 431 KTGMKIFESMKERYGVEPGPEHYACLVDLLGRAGMVECAYDFIKRMPFPPTISIWGALL 488
           K G ++      + G+ P       L+D+  + G +  A      M     +S W  L+
Sbjct: 492 KLGSQV-HCYSLKTGLAPEQFIKNGLIDMYAKCGSLGQARRIFDSMDNRDVVS-WSTLI 546

BLAST of Cucsa.254330 vs. TAIR10
Match: AT3G15130.1 (AT3G15130.1 Tetratricopeptide repeat (TPR)-like superfamily protein)

HSP 1 Score: 367.5 bits (942), Expect = 1.6e-101
Identity = 209/605 (34.55%), Postives = 329/605 (54.38%), Query Frame = 1

Query: 6   QNSLASVVELAVSVRSSLLGRAAHAQILKTLKTPFPAFLYNHLVNMYAKLDHLNSAKLIL 65
           + +L S++ +      S  G   H  +LK+  +       N+L++MY K      A  + 
Sbjct: 6   RQNLVSILRVCTRKGLSDQGGQVHCYLLKS-GSGLNLITSNYLIDMYCKCREPLMAYKVF 65

Query: 66  ELAPCRSVVTWTALIAGSVQNGCFVSALLHFSDMLSDCVRPNDFTFPCVLKASTGLRMDT 125
           +  P R+VV+W+AL++G V NG    +L  FS+M    + PN+FTF   LKA   L    
Sbjct: 66  DSMPERNVVSWSALMSGHVLNGDLKGSLSLFSEMGRQGIYPNEFTFSTNLKACGLLNALE 125

Query: 126 TGKQLHALAVKEGLINDVFVGCSVFDMYSKLGFLNDAYKVFDEMPHRNLETWNAYISNSV 185
            G Q+H   +K G    V VG S+ DMYSK G +N+A KVF  +  R+L +WNA I+  V
Sbjct: 126 KGLQIHGFCLKIGFEMMVEVGNSLVDMYSKCGRINEAEKVFRRIVDRSLISWNAMIAGFV 185

Query: 186 LHGRPEDSVIAF--IELLRVGGKPDSITFCAFLNACSDKLGLGPGCQLHGFIIRSGYG-- 245
             G    ++  F  ++   +  +PD  T  + L ACS    +  G Q+HGF++RSG+   
Sbjct: 186 HAGYGSKALDTFGMMQEANIKERPDEFTLTSLLKACSSTGMIYAGKQIHGFLVRSGFHCP 245

Query: 246 QNVSVSNGLIDFYGKCGEVECSEMVFDRMGERNSVSWSSLIAAYVQNNEEEKASCLFLRA 305
            + +++  L+D Y KCG +  +   FD++ E+  +SWSSLI  Y Q  E  +A  LF R 
Sbjct: 246 SSATITGSLVDLYVKCGYLFSARKAFDQIKEKTMISWSSLILGYAQEGEFVEAMGLFKRL 305

Query: 306 RKEDIEPTDFMVSSVLCACAGLSEIEFGRSVQALAVKACVEQNIFVASALVDMYGKCGSI 365
           ++ + +   F +SS++   A  + +  G+ +QALAVK        V +++VDMY KCG +
Sbjct: 306 QELNSQIDSFALSSIIGVFADFALLRQGKQMQALAVKLPSGLETSVLNSVVDMYLKCGLV 365

Query: 366 DNAEQAFNAMPERNLVSWNALLGGYAHQGHANKAVALLEEMTSAAGIVPSYVSLICALSA 425
           D AE+ F  M  ++++SW  ++ GY   G   K+V +  EM     I P  V  +  LSA
Sbjct: 366 DEAEKCFAEMQLKDVISWTVVITGYGKHGLGKKSVRIFYEMLRH-NIEPDEVCYLAVLSA 425

Query: 426 CSRAGDLKTGMKIFESMKERYGVEPGPEHYACLVDLLGRAGMVECAYDFIKRMPFPPTIS 485
           CS +G +K G ++F  + E +G++P  EHYAC+VDLLGRAG ++ A   I  MP  P + 
Sbjct: 426 CSHSGMIKEGEELFSKLLETHGIKPRVEHYACVVDLLGRAGRLKEAKHLIDTMPIKPNVG 485

Query: 486 IWGALLGACRMHGKPELGKLAAEKLFELDPKDSGNHVVLSNMFAATGRWEEVTVVRNEMK 545
           IW  LL  CR+HG  ELGK   + L  +D K+  N+V++SN++   G W E    R    
Sbjct: 486 IWQTLLSLCRVHGDIELGKEVGKILLRIDAKNPANYVMMSNLYGQAGYWNEQGNARELGN 545

Query: 546 EVGIKKGAGFSWITVDSRIHMFQAKDKSHEKDPEIQDILGKLRKEMQDAAGCIADPNYAL 605
             G+KK AG SW+ ++  +H F++ + SH   P IQ+ L +  + +++  G +    + L
Sbjct: 546 IKGLKKEAGMSWVEIEREVHFFRSGEDSHPLTPVIQETLKEAERRLREELGYVYGLKHEL 605

Query: 606 FEVSN 607
            ++ +
Sbjct: 606 HDIDD 608

BLAST of Cucsa.254330 vs. NCBI nr
Match: gi|449433319|ref|XP_004134445.1| (PREDICTED: pentatricopeptide repeat-containing protein At4g14850 [Cucumis sativus])

HSP 1 Score: 1223.0 bits (3163), Expect = 0.0e+00
Identity = 606/606 (100.00%), Postives = 606/606 (100.00%), Query Frame = 1

Query: 1   MPFLSQNSLASVVELAVSVRSSLLGRAAHAQILKTLKTPFPAFLYNHLVNMYAKLDHLNS 60
           MPFLSQNSLASVVELAVSVRSSLLGRAAHAQILKTLKTPFPAFLYNHLVNMYAKLDHLNS
Sbjct: 1   MPFLSQNSLASVVELAVSVRSSLLGRAAHAQILKTLKTPFPAFLYNHLVNMYAKLDHLNS 60

Query: 61  AKLILELAPCRSVVTWTALIAGSVQNGCFVSALLHFSDMLSDCVRPNDFTFPCVLKASTG 120
           AKLILELAPCRSVVTWTALIAGSVQNGCFVSALLHFSDMLSDCVRPNDFTFPCVLKASTG
Sbjct: 61  AKLILELAPCRSVVTWTALIAGSVQNGCFVSALLHFSDMLSDCVRPNDFTFPCVLKASTG 120

Query: 121 LRMDTTGKQLHALAVKEGLINDVFVGCSVFDMYSKLGFLNDAYKVFDEMPHRNLETWNAY 180
           LRMDTTGKQLHALAVKEGLINDVFVGCSVFDMYSKLGFLNDAYKVFDEMPHRNLETWNAY
Sbjct: 121 LRMDTTGKQLHALAVKEGLINDVFVGCSVFDMYSKLGFLNDAYKVFDEMPHRNLETWNAY 180

Query: 181 ISNSVLHGRPEDSVIAFIELLRVGGKPDSITFCAFLNACSDKLGLGPGCQLHGFIIRSGY 240
           ISNSVLHGRPEDSVIAFIELLRVGGKPDSITFCAFLNACSDKLGLGPGCQLHGFIIRSGY
Sbjct: 181 ISNSVLHGRPEDSVIAFIELLRVGGKPDSITFCAFLNACSDKLGLGPGCQLHGFIIRSGY 240

Query: 241 GQNVSVSNGLIDFYGKCGEVECSEMVFDRMGERNSVSWSSLIAAYVQNNEEEKASCLFLR 300
           GQNVSVSNGLIDFYGKCGEVECSEMVFDRMGERNSVSWSSLIAAYVQNNEEEKASCLFLR
Sbjct: 241 GQNVSVSNGLIDFYGKCGEVECSEMVFDRMGERNSVSWSSLIAAYVQNNEEEKASCLFLR 300

Query: 301 ARKEDIEPTDFMVSSVLCACAGLSEIEFGRSVQALAVKACVEQNIFVASALVDMYGKCGS 360
           ARKEDIEPTDFMVSSVLCACAGLSEIEFGRSVQALAVKACVEQNIFVASALVDMYGKCGS
Sbjct: 301 ARKEDIEPTDFMVSSVLCACAGLSEIEFGRSVQALAVKACVEQNIFVASALVDMYGKCGS 360

Query: 361 IDNAEQAFNAMPERNLVSWNALLGGYAHQGHANKAVALLEEMTSAAGIVPSYVSLICALS 420
           IDNAEQAFNAMPERNLVSWNALLGGYAHQGHANKAVALLEEMTSAAGIVPSYVSLICALS
Sbjct: 361 IDNAEQAFNAMPERNLVSWNALLGGYAHQGHANKAVALLEEMTSAAGIVPSYVSLICALS 420

Query: 421 ACSRAGDLKTGMKIFESMKERYGVEPGPEHYACLVDLLGRAGMVECAYDFIKRMPFPPTI 480
           ACSRAGDLKTGMKIFESMKERYGVEPGPEHYACLVDLLGRAGMVECAYDFIKRMPFPPTI
Sbjct: 421 ACSRAGDLKTGMKIFESMKERYGVEPGPEHYACLVDLLGRAGMVECAYDFIKRMPFPPTI 480

Query: 481 SIWGALLGACRMHGKPELGKLAAEKLFELDPKDSGNHVVLSNMFAATGRWEEVTVVRNEM 540
           SIWGALLGACRMHGKPELGKLAAEKLFELDPKDSGNHVVLSNMFAATGRWEEVTVVRNEM
Sbjct: 481 SIWGALLGACRMHGKPELGKLAAEKLFELDPKDSGNHVVLSNMFAATGRWEEVTVVRNEM 540

Query: 541 KEVGIKKGAGFSWITVDSRIHMFQAKDKSHEKDPEIQDILGKLRKEMQDAAGCIADPNYA 600
           KEVGIKKGAGFSWITVDSRIHMFQAKDKSHEKDPEIQDILGKLRKEMQDAAGCIADPNYA
Sbjct: 541 KEVGIKKGAGFSWITVDSRIHMFQAKDKSHEKDPEIQDILGKLRKEMQDAAGCIADPNYA 600

Query: 601 LFEVSN 607
           LFEVSN
Sbjct: 601 LFEVSN 606

BLAST of Cucsa.254330 vs. NCBI nr
Match: gi|659076423|ref|XP_008438671.1| (PREDICTED: pentatricopeptide repeat-containing protein At4g14850 [Cucumis melo])

HSP 1 Score: 1199.9 bits (3103), Expect = 0.0e+00
Identity = 593/606 (97.85%), Postives = 600/606 (99.01%), Query Frame = 1

Query: 1   MPFLSQNSLASVVELAVSVRSSLLGRAAHAQILKTLKTPFPAFLYNHLVNMYAKLDHLNS 60
           MPFLS+NSLASVVELAVSVRSSLLGRAAHAQILKTLKTPFPAFLYNHLVNMYAKLDHLNS
Sbjct: 1   MPFLSKNSLASVVELAVSVRSSLLGRAAHAQILKTLKTPFPAFLYNHLVNMYAKLDHLNS 60

Query: 61  AKLILELAPCRSVVTWTALIAGSVQNGCFVSALLHFSDMLSDCVRPNDFTFPCVLKASTG 120
           AKLILELAPCRSVVTWTALIAGSVQNGCFVSALLHFSDMLSDCVRPNDFTFPCVLKASTG
Sbjct: 61  AKLILELAPCRSVVTWTALIAGSVQNGCFVSALLHFSDMLSDCVRPNDFTFPCVLKASTG 120

Query: 121 LRMDTTGKQLHALAVKEGLINDVFVGCSVFDMYSKLGFLNDAYKVFDEMPHRNLETWNAY 180
           LRMD TGKQLHALAVKEGLINDVFVGCSVFDMYSKLGFLNDAYK+FDEMP RNLETWNAY
Sbjct: 121 LRMDMTGKQLHALAVKEGLINDVFVGCSVFDMYSKLGFLNDAYKLFDEMPQRNLETWNAY 180

Query: 181 ISNSVLHGRPEDSVIAFIELLRVGGKPDSITFCAFLNACSDKLGLGPGCQLHGFIIRSGY 240
           I+NSVLHGRPEDS IAFIELLRVG KPDSITFCAFLNACSDKLGLGPGCQLHGF+IRSGY
Sbjct: 181 ITNSVLHGRPEDSAIAFIELLRVGEKPDSITFCAFLNACSDKLGLGPGCQLHGFVIRSGY 240

Query: 241 GQNVSVSNGLIDFYGKCGEVECSEMVFDRMGERNSVSWSSLIAAYVQNNEEEKASCLFLR 300
           GQNVSVSNGLIDFYGKCGEVECSEMVFDRMGERNSVSWSSLIAAYVQNNEEEKASCLFLR
Sbjct: 241 GQNVSVSNGLIDFYGKCGEVECSEMVFDRMGERNSVSWSSLIAAYVQNNEEEKASCLFLR 300

Query: 301 ARKEDIEPTDFMVSSVLCACAGLSEIEFGRSVQALAVKACVEQNIFVASALVDMYGKCGS 360
           ARKEDIEPTDFMVSSVLCACAGLSEIEFGRSVQALAVKACVEQNIFVASALVDMYGKCGS
Sbjct: 301 ARKEDIEPTDFMVSSVLCACAGLSEIEFGRSVQALAVKACVEQNIFVASALVDMYGKCGS 360

Query: 361 IDNAEQAFNAMPERNLVSWNALLGGYAHQGHANKAVALLEEMTSAAGIVPSYVSLICALS 420
           IDNA QAFNAMPERNLVSWNALLGGYAHQGHANKAVALLEEMTSAAGIVPSYVSLICALS
Sbjct: 361 IDNAVQAFNAMPERNLVSWNALLGGYAHQGHANKAVALLEEMTSAAGIVPSYVSLICALS 420

Query: 421 ACSRAGDLKTGMKIFESMKERYGVEPGPEHYACLVDLLGRAGMVECAYDFIKRMPFPPTI 480
           ACSRAGDLKTGMKIFESMKERYGVEPGPEHYACLVDLLGRAGMVECAYDFIKRMPFPPTI
Sbjct: 421 ACSRAGDLKTGMKIFESMKERYGVEPGPEHYACLVDLLGRAGMVECAYDFIKRMPFPPTI 480

Query: 481 SIWGALLGACRMHGKPELGKLAAEKLFELDPKDSGNHVVLSNMFAATGRWEEVTVVRNEM 540
           SIWGALLGACRMHGKPELGKLAAEKLFELDPKDSGNHVVLSNMFAATGRWEE TVVRNEM
Sbjct: 481 SIWGALLGACRMHGKPELGKLAAEKLFELDPKDSGNHVVLSNMFAATGRWEEATVVRNEM 540

Query: 541 KEVGIKKGAGFSWITVDSRIHMFQAKDKSHEKDPEIQDILGKLRKEMQDAAGCIADPNYA 600
           KEVGIKKGAGFSWITVDSRIH+FQAKDKSHEKDPEIQ++LGKLRKEMQDAAGCIADPNYA
Sbjct: 541 KEVGIKKGAGFSWITVDSRIHIFQAKDKSHEKDPEIQNMLGKLRKEMQDAAGCIADPNYA 600

Query: 601 LFEVSN 607
           LFEVSN
Sbjct: 601 LFEVSN 606

BLAST of Cucsa.254330 vs. NCBI nr
Match: gi|645235511|ref|XP_008224305.1| (PREDICTED: pentatricopeptide repeat-containing protein At4g14850 [Prunus mume])

HSP 1 Score: 855.9 bits (2210), Expect = 4.3e-245
Identity = 422/605 (69.75%), Postives = 508/605 (83.97%), Query Frame = 1

Query: 1   MPFLSQNSLASVVELAVSVRSSLLGRAAHAQILKTLKTPFPAFLYNHLVNMYAKLDHLNS 60
           MPFL+ NSLAS+V+ A+S +S+LLGRAAHAQ+++TL TP P+FL NHLVNMY+KLD  +S
Sbjct: 1   MPFLTPNSLASLVQSALSTQSTLLGRAAHAQMIRTLDTPLPSFLSNHLVNMYSKLDLPDS 60

Query: 61  AKLILELAPCRSVVTWTALIAGSVQNGCFVSALLHFSDMLSDCVRPNDFTFPCVLKASTG 120
           A+L+L+L P RSVVTWTALIAGSVQNG F SA+LHF++ML + V+PNDFTFPC  KAS  
Sbjct: 61  AQLVLQLNPSRSVVTWTALIAGSVQNGHFASAILHFANMLRESVQPNDFTFPCAFKASGS 120

Query: 121 LRMDTTGKQLHALAVKEGLINDVFVGCSVFDMYSKLGFLNDAYKVFDEMPHRNLETWNAY 180
           LR+  TGKQ+HALAVK G I DVFVGCS FDMY K G  ++A KVFDEMP RNL TWNAY
Sbjct: 121 LRLPATGKQVHALAVKAGQICDVFVGCSAFDMYCKTGLRDEARKVFDEMPERNLATWNAY 180

Query: 181 ISNSVLHGRPEDSVIAFIELLRVGGKPDSITFCAFLNACSDKLGLGPGCQLHGFIIRSGY 240
           +SN+VL GRP ++V  FIE LR GG+P+SITFCAFLNACSD   L  G QLHGF++R G+
Sbjct: 181 MSNAVLDGRPLNAVYKFIEFLRAGGEPNSITFCAFLNACSDTSNLELGRQLHGFVMRCGF 240

Query: 241 GQNVSVSNGLIDFYGKCGEVECSEMVFDRMGERNSVSWSSLIAAYVQNNEEEKASCLFLR 300
           G++VSV NGLIDFYGKC EV  S MVFD + +RN VSW SL+AA VQN+EEE A  LFLR
Sbjct: 241 GKDVSVLNGLIDFYGKCREVASSMMVFDTIDKRNDVSWCSLVAACVQNDEEEMACELFLR 300

Query: 301 ARKEDIEPTDFMVSSVLCACAGLSEIEFGRSVQALAVKACVEQNIFVASALVDMYGKCGS 360
           ARKE +EPTDFMVSSVL AC+GL+ +E GRSV A+AVKACVE N+FV SALVDMYGKCGS
Sbjct: 301 ARKEGVEPTDFMVSSVLSACSGLAWLEQGRSVHAIAVKACVEGNLFVGSALVDMYGKCGS 360

Query: 361 IDNAEQAFNAMPERNLVSWNALLGGYAHQGHANKAVALLEEMT-SAAGIVPSYVSLICAL 420
           I++A++AFN MP RNL+SWNA++GGYAHQGHAN A+AL EEMT  +  + P+YV+L+C L
Sbjct: 361 IEDAKRAFNGMPSRNLISWNAMVGGYAHQGHANMALALFEEMTVRSHEVKPNYVTLVCVL 420

Query: 421 SACSRAGDLKTGMKIFESMKERYGVEPGPEHYACLVDLLGRAGMVECAYDFIKRMPFPPT 480
           SACSRAG ++TGM+IFESMK +YG+EPG EHYAC+VDLLGRAGMVE AY+FI +MP  PT
Sbjct: 421 SACSRAGAVETGMQIFESMKAKYGIEPGAEHYACVVDLLGRAGMVERAYEFITKMPICPT 480

Query: 481 ISIWGALLGACRMHGKPELGKLAAEKLFELDPKDSGNHVVLSNMFAATGRWEEVTVVRNE 540
           ISIWGALLGAC+M+ K ELG++AA+KLFELDPKDSGNHV+LSNMFAA GRWEE T+VR E
Sbjct: 481 ISIWGALLGACKMYRKTELGRVAADKLFELDPKDSGNHVILSNMFAAAGRWEEATLVRKE 540

Query: 541 MKEVGIKKGAGFSWITVDSRIHMFQAKDKSHEKDPEIQDILGKLRKEMQDAAGCIADPNY 600
           MK+VGIKKGAG+SWI V + +H+FQAKD SHE++ EIQ +L KLR+EM + AG IAD N+
Sbjct: 541 MKDVGIKKGAGYSWIAVKNAVHVFQAKDTSHERNSEIQAMLTKLRREM-EKAGYIADTNF 600

Query: 601 ALFEV 605
           ALF++
Sbjct: 601 ALFDL 604

BLAST of Cucsa.254330 vs. NCBI nr
Match: gi|1009141439|ref|XP_015888196.1| (PREDICTED: pentatricopeptide repeat-containing protein At4g14850 [Ziziphus jujuba])

HSP 1 Score: 842.8 bits (2176), Expect = 3.7e-241
Identity = 412/605 (68.10%), Postives = 500/605 (82.64%), Query Frame = 1

Query: 1   MPFLSQNSLASVVELAVSVRSSLLGRAAHAQILKTLKTPFPAFLYNHLVNMYAKLDHLNS 60
           MPFLS NSLA++VE A+S  SSL+GRAAHAQI+KTL TP P+FL NHLVNMY+KLD  NS
Sbjct: 1   MPFLSPNSLAALVESAISTCSSLIGRAAHAQIIKTLDTPLPSFLSNHLVNMYSKLDLPNS 60

Query: 61  AKLILELAPCRSVVTWTALIAGSVQNGCFVSALLHFSDMLSDCVRPNDFTFPCVLKASTG 120
           A+L+L L P R+VVTWT+LIAGSVQNG F SALLHF +M  +C++PNDFTFPC  KAS  
Sbjct: 61  AQLVLSLTPSRTVVTWTSLIAGSVQNGRFASALLHFVNMRRECIQPNDFTFPCAFKASAS 120

Query: 121 LRMDTTGKQLHALAVKEGLINDVFVGCSVFDMYSKLGFLNDAYKVFDEMPHRNLETWNAY 180
           LRM  TGKQ+HA+AVK G I DVFVGCS FDMY K G  ++A K+FDEMP RNL TWNAY
Sbjct: 121 LRMSGTGKQVHAVAVKTGQIRDVFVGCSAFDMYCKTGIRDEANKMFDEMPERNLATWNAY 180

Query: 181 ISNSVLHGRPEDSVIAFIELLRVGGKPDSITFCAFLNACSDKLGLGPGCQLHGFIIRSGY 240
           ISN+VL GRP+ ++  FIE +R GG+P+SITFCAFLNACSD   L  G QLHGF+IR G+
Sbjct: 181 ISNTVLDGRPKIAIEKFIEFVRAGGEPNSITFCAFLNACSDISDLELGRQLHGFVIRCGF 240

Query: 241 GQNVSVSNGLIDFYGKCGEVECSEMVFDRMGERNSVSWSSLIAAYVQNNEEEKASCLFLR 300
           G++VSV NGLIDFYGKC EV  S+ VFD +  RN VSW S++A YVQN+EEE+A  +FL+
Sbjct: 241 GKDVSVMNGLIDFYGKCREVGSSQTVFDSISLRNDVSWCSMVAVYVQNDEEERACGVFLQ 300

Query: 301 ARKEDIEPTDFMVSSVLCACAGLSEIEFGRSVQALAVKACVEQNIFVASALVDMYGKCGS 360
           ARKE IEPTDFMVSSVL ACAG++  E GRSV ALA+KACVE NIFV SALVDMYGKCGS
Sbjct: 301 ARKEGIEPTDFMVSSVLSACAGMAGFELGRSVHALALKACVEGNIFVGSALVDMYGKCGS 360

Query: 361 IDNAEQAFNAMPERNLVSWNALLGGYAHQGHANKAVALLEEMTSAA-GIVPSYVSLICAL 420
           ID+ EQAF  MPE+NL++WNA+L GYAHQGHA+ A+ L EE++S + GI P+YV+L+C L
Sbjct: 361 IDDCEQAFEEMPEKNLITWNAMLCGYAHQGHADMALTLFEEISSGSHGIRPNYVTLVCVL 420

Query: 421 SACSRAGDLKTGMKIFESMKERYGVEPGPEHYACLVDLLGRAGMVECAYDFIKRMPFPPT 480
           SACSRAG +K G++IF SM+ +YG+EPG EHYAC+VDLLGRAGMVE AY+FIK+MP  PT
Sbjct: 421 SACSRAGMVKKGIEIFGSMRVKYGIEPGAEHYACVVDLLGRAGMVEHAYEFIKKMPICPT 480

Query: 481 ISIWGALLGACRMHGKPELGKLAAEKLFELDPKDSGNHVVLSNMFAATGRWEEVTVVRNE 540
           ISIWGALLGACR++ KP++GK+AA+ LFELDPKDSGNHVVLSNMFA+ GRWEE T+VR E
Sbjct: 481 ISIWGALLGACRVYRKPDIGKIAADHLFELDPKDSGNHVVLSNMFASAGRWEEATLVRKE 540

Query: 541 MKEVGIKKGAGFSWITVDSRIHMFQAKDKSHEKDPEIQDILGKLRKEMQDAAGCIADPNY 600
           MK+VGIKKGAG SW+ V + +H+FQAKD  HE++ EIQ +L KLR+EM++ AG + D ++
Sbjct: 541 MKDVGIKKGAGCSWVAVKNAVHVFQAKDTFHERNSEIQAMLSKLRREMKE-AGYVPDTDF 600

Query: 601 ALFEV 605
           ALF++
Sbjct: 601 ALFDL 604

BLAST of Cucsa.254330 vs. NCBI nr
Match: gi|225432688|ref|XP_002282622.1| (PREDICTED: pentatricopeptide repeat-containing protein At4g14850 [Vitis vinifera])

HSP 1 Score: 827.8 bits (2137), Expect = 1.2e-236
Identity = 409/605 (67.60%), Postives = 488/605 (80.66%), Query Frame = 1

Query: 1   MPFLSQNSLASVVELAVSVRSSLLGRAAHAQILKTLKTPFPAFLYNHLVNMYAKLDHLNS 60
           MPFLS NSLAS+VE AVS + S LGRAAHAQI+KTL  P P+F+YNHLVNMY+KLD  NS
Sbjct: 1   MPFLSPNSLASLVESAVSTQCSRLGRAAHAQIIKTLDNPLPSFIYNHLVNMYSKLDRPNS 60

Query: 61  AKLILELAPCRSVVTWTALIAGSVQNGCFVSALLHFSDMLSDCVRPNDFTFPCVLKASTG 120
           A+L+L L P RSVVTWTALIAGSVQNG F SAL HFS+M  D ++PNDFTFPC  KAS  
Sbjct: 61  AQLLLSLTPNRSVVTWTALIAGSVQNGRFTSALFHFSNMRRDSIQPNDFTFPCAFKASGS 120

Query: 121 LRMDTTGKQLHALAVKEGLINDVFVGCSVFDMYSKLGFLNDAYKVFDEMPHRNLETWNAY 180
           LR    GKQ+HALAVK G I+DVFVGCS FDMYSK G   +A K+FDEMP RN+ TWNAY
Sbjct: 121 LRSPLVGKQVHALAVKAGQISDVFVGCSAFDMYSKAGLTEEARKMFDEMPERNIATWNAY 180

Query: 181 ISNSVLHGRPEDSVIAFIELLRVGGKPDSITFCAFLNACSDKLGLGPGCQLHGFIIRSGY 240
           +SNSVL GR +D++ AFIE    G +P+ ITFCAFLNAC+    L  G QLHGF+++SG+
Sbjct: 181 LSNSVLEGRYDDALTAFIEFRHEGWEPNLITFCAFLNACAGASYLRLGRQLHGFVLQSGF 240

Query: 241 GQNVSVSNGLIDFYGKCGEVECSEMVFDRMGERNSVSWSSLIAAYVQNNEEEKASCLFLR 300
             +VSV+NGLIDFYGKC +V CSE++F  + + N VSW S+I +YVQN+EEEKA  +FLR
Sbjct: 241 EADVSVANGLIDFYGKCHQVGCSEIIFSGISKPNDVSWCSMIVSYVQNDEEEKACLVFLR 300

Query: 301 ARKEDIEPTDFMVSSVLCACAGLSEIEFGRSVQALAVKACVEQNIFVASALVDMYGKCGS 360
           ARKE IEPTDFMVSSVL ACAGLS +E G+SV  LAVKACV  NIFV SALVDMYGKCGS
Sbjct: 301 ARKEGIEPTDFMVSSVLSACAGLSVLEVGKSVHTLAVKACVVGNIFVGSALVDMYGKCGS 360

Query: 361 IDNAEQAFNAMPERNLVSWNALLGGYAHQGHANKAVALLEEMTSAA-GIVPSYVSLICAL 420
           I++AE+AF+ MPERNLV+WNA++GGYAHQG A+ AV L +EMT  +  + P+YV+ +C L
Sbjct: 361 IEDAERAFDEMPERNLVTWNAMIGGYAHQGQADMAVTLFDEMTCGSHRVAPNYVTFVCVL 420

Query: 421 SACSRAGDLKTGMKIFESMKERYGVEPGPEHYACLVDLLGRAGMVECAYDFIKRMPFPPT 480
           SACSRAG +  GM+IFESM+ RYG+EPG EHYAC+VDLLGRAGMVE AY FIK+MP  PT
Sbjct: 421 SACSRAGSVNVGMEIFESMRGRYGIEPGAEHYACVVDLLGRAGMVEQAYQFIKKMPIRPT 480

Query: 481 ISIWGALLGACRMHGKPELGKLAAEKLFELDPKDSGNHVVLSNMFAATGRWEEVTVVRNE 540
           +S+WGALLGA +M GK ELGK+AA+ LFELDP DSGNHV+LSNMFAA GRWEE T+VR E
Sbjct: 481 VSVWGALLGASKMFGKSELGKVAADNLFELDPLDSGNHVLLSNMFAAAGRWEEATLVRKE 540

Query: 541 MKEVGIKKGAGFSWITVDSRIHMFQAKDKSHEKDPEIQDILGKLRKEMQDAAGCIADPNY 600
           MK+VGIKKGAG SWIT  + +H+FQAKD SHE++ EIQ +L KLR EM +AAG I D ++
Sbjct: 541 MKDVGIKKGAGCSWITAGNAVHVFQAKDTSHERNSEIQAMLAKLRGEM-EAAGYIPDTSF 600

Query: 601 ALFEV 605
           ALF++
Sbjct: 601 ALFDL 604