Cucsa.253380 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.253380 vs. Swiss-Prot
Match: MTEF4_ARATH (Transcription termination factor MTERF4, chloroplastic OS=Arabidopsis thaliana GN=MTERF4 PE=1 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 9.2e-08
Identity = 62/254 (24.41%), Postives = 119/254 (46.85%), Query Frame = 1

Query: 83  VPLILSANPEILFPILLFFKSKGLSSPAITKLVCFA-------------PQILKRSLNQE 142
           VP +L   PE+L      FK +G  S ++  LV                P+IL   + + 
Sbjct: 232 VPRVLERYPEVLG-----FKLEGTMSTSVAYLVGIGVARREIGGILTRYPEILGMRVARI 291

Query: 143 IIPFFDYIQAVLGTVEKTVAT-IKRFPRILGWNLRISVGPNIEILKQFGVPDSNISTYLQ 202
           I P  +Y++ VLG      A  I++ P ILG+ L  +V PN++IL+ F V ++++ + + 
Sbjct: 292 IKPLVEYLE-VLGIPRLAAARLIEKRPHILGFELDDTVKPNVQILQDFNVRETSLPSIIA 351

Query: 203 RQPKVFSTSSIRFKEIVER---VTEMGFNPQRL-QFIVAVHALRSLTKSSWDKKLEVYRK 262
           + P++     ++ K   +R    + +  NP+ L   I  +    SL++S   K ++   K
Sbjct: 352 QYPEIIGI-DLKPKLDTQRKLLCSAIHLNPEDLGSLIERMPQFVSLSESPMLKHIDFLTK 411

Query: 263 WGLSEEEFYLAFRKYPWCMALSEEKINDTMDFFINKMGRESSLVARRPSLLSYSLKKRLF 319
            G S ++        P  +AL+   +  + ++F  +M R    +   P+  +Y L+  + 
Sbjct: 412 CGFSIDQTREMVIGCPQVLALNLGIMKLSFEYFQKEMKRPLQDLVDFPAFFTYGLESTVK 471

HSP 2 Score: 58.5 bits (140), Expect = 3.5e-07
Identity = 57/281 (20.28%), Postives = 129/281 (45.91%), Query Frame = 1

Query: 438 VIGLLASHGFSESQISDLAKRYPSLFSANPDKTILPKLLFFQSKGLSSPEIVKFVCSVPR 497
           V+  L       S +  + +RYP +     + T+   + +    G++  EI   +   P 
Sbjct: 218 VVKYLQGLDIKPSDVPRVLERYPEVLGFKLEGTMSTSVAYLVGIGVARREIGGILTRYPE 277

Query: 498 VLAGSLNKRIIPAFDYIQAVLGSEEKTLAA-IKRSADILGWDLQISVGPNIEILKQTGVP 557
           +L   + + I P  +Y++ VLG      A  I++   ILG++L  +V PN++IL+   V 
Sbjct: 278 ILGMRVARIIKPLVEYLE-VLGIPRLAAARLIEKRPHILGFELDDTVKPNVQILQDFNVR 337

Query: 558 DSNISSYLQQQPKMFLTSSIRFKEAVER---VTEMGFNPQQM-QFVVAVFCLRAMTKSTL 617
           ++++ S + Q P++ +   ++ K   +R    + +  NP+ +   +  +    ++++S +
Sbjct: 338 ETSLPSIIAQYPEI-IGIDLKPKLDTQRKLLCSAIHLNPEDLGSLIERMPQFVSLSESPM 397

Query: 618 DKKVEVYRKWGLSEEEIRLAFKKNPWCMMISEDKINGAMDYFVNKIGCQSSYVARRPGLT 677
            K ++   K G S ++ R      P  + ++   +  + +YF  ++      +   P   
Sbjct: 398 LKHIDFLTKCGFSIDQTREMVIGCPQVLALNLGIMKLSFEYFQKEMKRPLQDLVDFPAFF 457

Query: 678 LYSLKKRLLPRGYIYQVLLSKGLIKKHEYLSSLFNSSENRF 714
            Y L+  + PR   ++ ++ KG+      L+ + N S+ +F
Sbjct: 458 TYGLESTVKPR---HKKIIKKGIKCS---LAWMLNCSDEKF 490

HSP 3 Score: 39.7 bits (91), Expect = 1.7e-01
Identity = 32/172 (18.60%), Postives = 76/172 (44.19%), Query Frame = 1

Query: 147 TVATIKRFPRILGWNLRISVGPNIEILKQFGVPDSNISTYLQRQPKVFSTS-SIRFKEIV 206
           T+  I  +P +LG +++ ++ P ++ L + GV  S  + +L+R P+V  +S  I    +V
Sbjct: 160 TIEDINNYPLVLGCSVKKNMVPVLDYLGKLGVRKSTFTEFLRRYPQVLHSSVVIDLAPVV 219

Query: 207 ERVTEMGFNPQRLQFIVAVH--ALRSLTKSSWDKKLEVYRKWGLSEEEFYLAFRKYPWCM 266
           + +  +   P  +  ++  +   L    + +    +      G++  E      +YP  +
Sbjct: 220 KYLQGLDIKPSDVPRVLERYPEVLGFKLEGTMSTSVAYLVGIGVARREIGGILTRYPEIL 279

Query: 267 ALSEEKINDTMDFFINKMG----RESSLVARRPSLLSYSLKKRLFPRGYVYQ 312
            +   +I   +  ++  +G      + L+ +RP +L + L   + P   + Q
Sbjct: 280 GMRVARIIKPLVEYLEVLGIPRLAAARLIEKRPHILGFELDDTVKPNVQILQ 331

BLAST of Cucsa.253380 vs. TrEMBL
Match: F6HDG2_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_05s0020g01630 PE=4 SV=1)

HSP 1 Score: 567.0 bits (1460), Expect = 3.4e-158
Identity = 311/703 (44.24%), Postives = 453/703 (64.44%), Query Frame = 1

Query: 42  SPKSASLASNVVQLKNNRK--AIIAFFENHGFSESQVSDLIKKVPLILSANPE-ILFPIL 101
           S K A  AS +++ +   K  +++AFF +HGFS+SQ S ++K +P +L+++P+  L P L
Sbjct: 67  SHKDALSASKLLRFETPEKPDSVLAFFNSHGFSKSQTSKIVKSLPRLLASDPDKTLLPKL 126

Query: 102 LFFKSKGLSSPAITKLVCFAPQILKRSLNQEIIPFFDYIQAVLGTVEKTVATIKRFPRIL 161
            FF SKG S P + K+V   P ILKRSL  +IIP F++++  L + E  +  +KRF RIL
Sbjct: 127 QFFYSKGASKPDVAKIVVSTPGILKRSLENQIIPSFNFLKDFLQSDEMAITVVKRFSRIL 186

Query: 162 GWNLRISVGPNIEILKQFGVPDSNISTYLQRQPKVFSTSSIRFKEIVERVTEMGFNPQRL 221
            ++L   V  N+  L++FGVP SNI+  L  +P  F  +   F++ +E V +MGFNP ++
Sbjct: 187 LFDLHTYVASNMNALQEFGVPKSNIAGLLMYRPMAFMVNPNLFRKNLEEVKKMGFNPSQM 246

Query: 222 QFIVAVHALRSLTKSSWDKKLEVYRKWGLSEEEFYLAFRKYPWCMALSEEKINDTMDFFI 281
           +F++A+ A+R+  +S W++K+++Y+KWG SEEE  LAF K PWCM  SE+KI   MDFF+
Sbjct: 247 KFVLAIQAMRAGGESCWERKIDIYKKWGWSEEEIRLAFTKSPWCMIYSEDKIMAKMDFFV 306

Query: 282 NKMGRESSLVARRPSLLSYSLKKRLFPRGYVYQVLLSKGLIKKEKNLDLLFEPPEKRFIE 341
           NKMGRESSL+A RP L+  SL+KR+ PR  V QVLLSKGLI K+ +L +LFE  EK F+E
Sbjct: 307 NKMGRESSLIAHRPFLIGLSLEKRIIPRYSVVQVLLSKGLINKDISLVVLFESTEKTFLE 366

Query: 342 KYINAHKEQVPGLLEL----YEQKLMNLEDEQAFVVSP-----FLLLNKEPTGLSSFSSH 401
           +++NA+KE+ P L++L    + +K   L+       SP       LL+      SS S+ 
Sbjct: 367 RFVNAYKEEAPQLIKLMMIGFLRKAPVLQVRFNLTASPTPQSLHFLLDHLYLFSSSSSTS 426

Query: 402 FLHSFFSHGFSESPLKSLRYLSNSSEIV-SSLKTASLANTAQLENNGKAVIGLLASHGFS 461
              S   H F+ S      YL NS  +     ++AS     +  +   +V+ L  SHGFS
Sbjct: 427 ISTSEKPHPFTVS------YLINSCGLSHKDARSASKYVHFETPDKPNSVLALFNSHGFS 486

Query: 462 ESQISDLAKRYPSLFSANPDKTILPKLLFFQSKGLSSPEIVKFVCSVPRVLAGSLNKRII 521
           ++Q S + K+ P L  ++PDKT+LPKL FF SKG S P+I K V   P +L  SL  +II
Sbjct: 487 KTQTSKIVKKEPQLLLSDPDKTLLPKLQFFYSKGASWPDIAKIVVCSPSILKRSLENQII 546

Query: 522 PAFDYIQAVLGSEEKTLAAIKRSADILGWDLQISVGPNIEILKQTGVPDSNISSYLQQQP 581
           P+F++ +  L S++  +  +KR + IL +DL   V  N+  L++ GVP SNI+  L  QP
Sbjct: 547 PSFNFFKDFLQSDKMAITVVKRFSRILLFDLHTYVASNMNALQEFGVPKSNIAGLLMNQP 606

Query: 582 KMFLTSSIRFKEAVERVTEMGFNPQQMQFVVAVFCLRAMTKSTLDKKVEVYRKWGLSEEE 641
             F+     F+E +E V +MG NP QM+FV+A+  +RA  KS+ ++K+++Y++WG SEEE
Sbjct: 607 MAFMVRPNLFRENLEEVKKMGSNPSQMKFVIAIQAIRAGGKSSWERKIDIYKRWGWSEEE 666

Query: 642 IRLAFKKNPWCMMISEDKINGAMDYFVNKIGCQSSYVARRPGLTLYSLKKRLLPRGYIYQ 701
           IRLAF K+PWCM+ SEDKI   MD+FVNK+G +SS +ARRP L   SL+KR++PR  + Q
Sbjct: 667 IRLAFTKSPWCMIYSEDKIMATMDFFVNKMGRESSSIARRPQLISPSLEKRIIPRYSVVQ 726

Query: 702 VLLSKGLIKKHEYLSSLFNSSENRFIKKFINPHKEQIPGLLEL 732
           VLLSKGLI K   LS++F S+E  F+ KF++ +KE+ P LL L
Sbjct: 727 VLLSKGLISKDFSLSAVFQSTEIMFLHKFVDVYKEEAPQLLNL 763

BLAST of Cucsa.253380 vs. TrEMBL
Match: F6HDG2_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_05s0020g01630 PE=4 SV=1)

HSP 1 Score: 287.3 bits (734), Expect = 5.2e-74
Identity = 160/374 (42.78%), Postives = 233/374 (62.30%), Query Frame = 1

Query: 366 QAFVVSPFLLLNKEPTGLS-SFSSHFLHSFFSHGFSESPLK-----SLRYLSNSSEIVSS 425
           +A V+     L   PT  S  F  H  + F S   S S  +     ++ YL +S  + S 
Sbjct: 10  KALVLQVRFTLTASPTPQSLHFLPHHFYPFSSSSTSISTSERSESFTVSYLIDSCGL-SH 69

Query: 426 LKTASLANTAQLENNGK--AVIGLLASHGFSESQISDLAKRYPSLFSANPDKTILPKLLF 485
               S +   + E   K  +V+    SHGFS+SQ S + K  P L +++PDKT+LPKL F
Sbjct: 70  KDALSASKLLRFETPEKPDSVLAFFNSHGFSKSQTSKIVKSLPRLLASDPDKTLLPKLQF 129

Query: 486 FQSKGLSSPEIVKFVCSVPRVLAGSLNKRIIPAFDYIQAVLGSEEKTLAAIKRSADILGW 545
           F SKG S P++ K V S P +L  SL  +IIP+F++++  L S+E  +  +KR + IL +
Sbjct: 130 FYSKGASKPDVAKIVVSTPGILKRSLENQIIPSFNFLKDFLQSDEMAITVVKRFSRILLF 189

Query: 546 DLQISVGPNIEILKQTGVPDSNISSYLQQQPKMFLTSSIRFKEAVERVTEMGFNPQQMQF 605
           DL   V  N+  L++ GVP SNI+  L  +P  F+ +   F++ +E V +MGFNP QM+F
Sbjct: 190 DLHTYVASNMNALQEFGVPKSNIAGLLMYRPMAFMVNPNLFRKNLEEVKKMGFNPSQMKF 249

Query: 606 VVAVFCLRAMTKSTLDKKVEVYRKWGLSEEEIRLAFKKNPWCMMISEDKINGAMDYFVNK 665
           V+A+  +RA  +S  ++K+++Y+KWG SEEEIRLAF K+PWCM+ SEDKI   MD+FVNK
Sbjct: 250 VLAIQAMRAGGESCWERKIDIYKKWGWSEEEIRLAFTKSPWCMIYSEDKIMAKMDFFVNK 309

Query: 666 IGCQSSYVARRPGLTLYSLKKRLLPRGYIYQVLLSKGLIKKHEYLSSLFNSSENRFIKKF 725
           +G +SS +A RP L   SL+KR++PR  + QVLLSKGLI K   L  LF S+E  F+++F
Sbjct: 310 MGRESSLIAHRPFLIGLSLEKRIIPRYSVVQVLLSKGLINKDISLVVLFESTEKTFLERF 369

Query: 726 INPHKEQIPGLLEL 732
           +N +KE+ P L++L
Sbjct: 370 VNAYKEEAPQLIKL 382

HSP 2 Score: 450.7 bits (1158), Expect = 3.5e-123
Identity = 272/741 (36.71%), Postives = 417/741 (56.28%), Query Frame = 1

Query: 2   YNLFRRIILPGSPSSVFTHGFSESPLK---SLRFLSTSSEIVS------------SPKSA 61
           Y++FR  +L G  +   +  F  S +K   SLRF+S SS   S            S + A
Sbjct: 3   YSIFRTSLLHGRRAMAASQSFKFSNIKISTSLRFISISSNGHSFTVSYLINKCGFSTELA 62

Query: 62  SLASNVVQLKNNRK--AIIAFFENHGFSESQVSDLIKKVPLILSANPE-ILFPILLFFKS 121
           S AS+ V  +   K  ++  F ENHGFS +Q+ +LIKK P +L  + E  L P + +  S
Sbjct: 63  SRASSYVHFETPEKPDSLFVFLENHGFSRTQILNLIKKRPRLLVCDTEKTLLPKIEYLHS 122

Query: 122 KGLSSPAITKLVCFAPQILKRSLNQEIIPFFDYIQAVLGTVEKTVATIKRFPRILGWNLR 181
            G S P + K++   P +L  SL  +IIP F+ ++ +  + +K +  IKRF  IL ++L 
Sbjct: 123 LGFSRPELAKILSSYPTLLMHSLKNQIIPNFNLLRNLFHSDDKAIKAIKRFTPILVYDLE 182

Query: 182 ISVGPNIEILKQFGVPDSNISTYLQRQPKVFSTSSIRFKEIVERVTEMGFNPQRLQFIVA 241
             + PN+ +L+  GVP+SNI   L RQP+    + +R KEIVE    MGF+P    F+  
Sbjct: 183 SYLYPNMNVLRVIGVPESNILVLLNRQPRSLLYNPVRLKEIVEEAERMGFDPSTKMFLSV 242

Query: 242 VHALRSLTKSSWDKKLEVYRKWGLSEEEFYLAFRKYPWCMALSEEKINDTMDFFINKMGR 301
           V AL+S+TKS+ +KK +VYR+WG S++E + A R++P CM +SE+K+   MDF + KMG 
Sbjct: 243 VIALKSMTKSTLEKKFDVYRRWGWSDQEIHEALRRHPLCMTVSEDKVMAIMDFLVKKMGY 302

Query: 302 ESSLVARRPSLLSYSLKKRLFPRGYVYQVLLSKGLIKKEKNLDLLFEPPEKRFIEKYINA 361
            S+L+A++PS+L  S +K + PR    + LLS+GL+   K L +LF+  EK FI  +++ 
Sbjct: 303 SSTLIAKQPSILRKSFRKNIIPRALFARELLSQGLVNDLK-LSVLFDTSEKVFIRMFVDR 362

Query: 362 HKEQVPGLLELYEQKLMNLEDEQAFVVSPFLLLNKEPTGLSSFSSHFLHSFF------SH 421
              + P LL+LY++KL   E +Q            EP    SFSS  L   F      SH
Sbjct: 363 FVNKAPELLKLYKEKLKISEKKQTL----------EPVPAVSFSSPILWGKFLILSLGSH 422

Query: 422 --GFSESPLKSLRYLSNSSEIVSSLKT-ASLANTAQLENNGKAVIGLLASHGFSESQISD 481
             G S     ++ YL N     + L + AS     +      ++   L +HG        
Sbjct: 423 KRGCSNGHSFTVSYLINKCGFSTELASRASSYVHFETPEKPDSLFAFLENHG-------- 482

Query: 482 LAKRYPSLFSANPDKTILPKLLFFQSKGLSSPEIVKFVCSVPRVLAGSLNKRIIPAFDYI 541
                  L   + +KT+LPK+ +  S G S P++ K + S P +L  SL  +IIP F+ +
Sbjct: 483 -------LLVCDTEKTLLPKIEYLHSLGFSRPKLAKILSSYPSLLMRSLKNQIIPNFNLL 542

Query: 542 QAVLGSEEKTLAAIKRSADILGWDLQISVGPNIEILKQTGVPDSNISSYLQQQPKMFLTS 601
           + +  S++K +  IKR   IL +DL+  + PN+ +L+  GVP+SNI   L +QP+  L +
Sbjct: 543 RNLFHSDDKAIKVIKRYTPILVYDLESYLYPNMNVLRGIGVPESNILVLLNRQPRSLLYN 602

Query: 602 SIRFKEAVERVTEMGFNPQQMQFVVAVFCLRAMTKSTLDKKVEVYRKWGLSEEEIRLAFK 661
            +R KE VE    MGF+P    F+  V  L++MTKSTL+KK +VYR+WG S++EI  AF+
Sbjct: 603 PVRLKEIVEEAERMGFDPSTKMFLSVVIALKSMTKSTLEKKFDVYRRWGWSDQEIHEAFR 662

Query: 662 KNPWCMMISEDKINGAMDYFVNKIGCQSSYVARRPGLTLYSLKKRLLPRGYIYQVLLSKG 716
           ++P CM +SEDK+   MD+ V K+G  S+ +A++P +   S +K ++PR    + LLS+G
Sbjct: 663 RHPLCMTVSEDKVMAIMDFLVKKLGYSSTLIAKQPSILWKSFRKNIVPRALFARELLSQG 716

BLAST of Cucsa.253380 vs. TrEMBL
Match: A0A0D2Q720_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_002G027700 PE=4 SV=1)

HSP 1 Score: 436.4 bits (1121), Expect = 6.9e-119
Identity = 261/703 (37.13%), Postives = 399/703 (56.76%), Query Frame = 1

Query: 42   SPKSASLASNVVQLKNNRK--AIIAFFENHGFSESQVSDLIKKVPLILSANPEILFPILL 101
            S + AS AS+ V  +   K  ++ AF ENHG              L++    + L P + 
Sbjct: 433  STELASRASSYVHFETPEKPDSLFAFLENHG--------------LLVCDTEKTLLPKIE 492

Query: 102  FFKSKGLSSPAITKLVCFAPQILKRSLNQEIIPFFDYIQAVLGTVEKTVATIKRFPRILG 161
            +  S G S P + K++   P +L RSL  +IIP F+ ++ +  + +K +  IKR+  IL 
Sbjct: 493  YLHSLGFSRPKLAKILSSYPSLLMRSLKNQIIPNFNLLRNLFHSDDKAIKVIKRYTPILV 552

Query: 162  WNLRISVGPNIEILKQFGVPDSNISTYLQRQPKVFSTSSIRFKEIVERVTEMGFNPQRLQ 221
            ++L   + PN+ +L+  GVP+SNI   L RQP+    + +R KEIVE    MGF+P    
Sbjct: 553  YDLESYLYPNMNVLRGIGVPESNILVLLNRQPRSLLYNPVRLKEIVEEAERMGFDPSTKM 612

Query: 222  FIVAVHALRSLTKSSWDKKLEVYRKWGLSEEEFYLAFRKYPWCMALSEEKINDTMDFFIN 281
            F+  V AL+S+TKS+ +KK +VYR+WG S++E + AFR++P CM +SE+K+   MDF + 
Sbjct: 613  FLSVVIALKSMTKSTLEKKFDVYRRWGWSDQEIHEAFRRHPLCMTVSEDKVMAIMDFLVK 672

Query: 282  KMGRESSLVARRPSLLSYSLKKRLFPRGYVYQVLLSKGLIKKEKNLDLLFEPPEKRFIEK 341
            K+G  S+L+A++PS+L  S +K + PR    + LLS+GL+   K L +LF+  EK FI  
Sbjct: 673  KLGYSSTLIAKQPSILWKSFRKNIVPRALFARELLSQGLVNDLK-LSVLFDTSEKVFIRI 732

Query: 342  YINAHKEQVPGLLELYEQKLMNLEDEQAFVVSPFLLLNKEPTGLSSFSSHFLHSFFSHGF 401
                H  Q              +   Q+F +S    + K+ T L  FS     + F+   
Sbjct: 733  TSFLHGRQA-------------MACPQSFNLS----MIKKSTTLRFFSKTSNQNSFT--- 792

Query: 402  SESPLKSLRYLSNSSEIVSSLKT-ASLANTAQLENNGKAVIGLLASHGFSESQISDLAKR 461
                   + YL N         + AS     +      ++   L +HGFS++QI +L KR
Sbjct: 793  -------VSYLMNKCGFTPEFASFASKYVHFETPERPDSLFAFLENHGFSKTQILNLIKR 852

Query: 462  YPSLFSANPDKTILPKLLFFQSKGLSSPEIVKFVCSVPRVLAGSLNKRIIPAFDYIQAVL 521
             P L   + +KT+LPKL FF S G S PE+ K + S P VL  SL K+IIP+F+ ++ + 
Sbjct: 853  RPRLLIYDTEKTLLPKLEFFYSIGFSRPELTKILTSYPAVLICSLKKQIIPSFNLLRNLF 912

Query: 522  GSEEKTLAAIKRSADILGWDLQISVGPNIEILKQTGVPDSNISSYLQQQPKMFLTSSIRF 581
             S++K +  IKR   I  ++ + ++ PN+ +L+  GVP+SNI   L  QP+  L   +R 
Sbjct: 913  QSDDKAIKTIKRYGAIFVYEFERNLIPNMNVLRGIGVPESNILMLLNHQPRPLLYDQVRL 972

Query: 582  KEAVERVTEMGFNPQQMQFVVAVFCLRAMTKSTLDKKVEVYRKWGLSEEEIRLAFKKNPW 641
            KE VE V  MGF+    +F+  V  L +M+KSTL+KK +VYR+WG S++EI  AF++ P 
Sbjct: 973  KEIVEEVKRMGFDSSTKKFIDVVIALSSMSKSTLEKKFDVYRRWGWSDQEIHEAFRRYPM 1032

Query: 642  CMMISEDKINGAMDYFVNKIGCQSSYVARRPGLTLYSLKKRLLPRGYIYQVLLSKGLIKK 701
            CM +SEDKI   MD+ VNK+G  S+ VA++  +   SL+KR++PR    + LLS+GL+  
Sbjct: 1033 CMAVSEDKIMAVMDFLVNKMGYSSTLVAKQSSILRQSLEKRIVPRALFARELLSQGLVTD 1088

Query: 702  HEYLSSLFNSSENRFIKKFINPHKEQIPGLLELYKERLMDSRR 742
             + LS LF++SE  F+ +F+N    + P LL+LYKE L  S +
Sbjct: 1093 FK-LSVLFHTSEKVFVDRFVN----KAPDLLKLYKENLNASEK 1088

HSP 2 Score: 432.6 bits (1111), Expect = 9.9e-118
Identity = 257/720 (35.69%), Postives = 412/720 (57.22%), Query Frame = 1

Query: 15   SSVFTHGFSESPLKSLRFLSTSSEIVSSPKSASLASNVVQLKNNRKA--IIAFFENHGFS 74
            SS   H F+ S L +   LST S + +S K        VQ +   +A  ++A   N+G +
Sbjct: 523  SSPKQHSFTVSYLMNSCGLSTESALSASRK--------VQFETPERADSVLALLRNYGCT 582

Query: 75   ESQVSDLIKKVPLILSANPE-ILFPILLFFKSKGLSSPAITKLVCFAPQILKRSLNQEII 134
             + +S ++ + PL+L+ANPE  L P L FF+S G S P +  +V  +P IL+RSL   +I
Sbjct: 583  NTHISKIVSRYPLLLTANPEKTLLPKLEFFRSVGFSGPDLASIVVSSPIILRRSLENHVI 642

Query: 135  PFFDYIQAVLGTVEKTVATIKRFPRILGWNLRISVGPNIEILKQFGVPDSNISTYLQRQP 194
            P ++++++V+   E  V   K+   I G N++ ++ PNI IL++ GVP SN+   +   P
Sbjct: 643  PSYNFLKSVVMVNENIVRAFKKTFWISGQNVQNAIAPNIAILEEIGVPMSNMKFLVTCHP 702

Query: 195  KVFSTSSIRFKEIVERVTEMGFNPQRLQFIVAVHALRSLTKSSWDKKLEVYRKWGLSEEE 254
             V S +  +F   V++V EMGFNP RL F+ A+     LT+S  + K+EVYR+WGL+++E
Sbjct: 703  NVVSQNREKFSRSVKKVIEMGFNPLRLSFLKAIEVSCQLTESMLEHKMEVYRRWGLTDDE 762

Query: 255  FYLAFRKYPWCMALSEEKINDTMDFFINKMGRESSLVARRPSLLSYSLKKRLFPRGYVYQ 314
                FR  P CM  SE+KI   MDF +NKMG E +  AR P++   SL+K+  PR    +
Sbjct: 763  IMSMFRLDPLCMKSSEKKIMSVMDFLVNKMGWEPAAFARYPTVFLCSLEKKXIPRCSAVK 822

Query: 315  VLLSKGLIKKEKNLDLLFEPPEKRFIEKYINAHKEQVPGLLELYEQKLMNLEDEQAFVVS 374
             L  KGL+KK+     L+   +K F +K++  +++  P LL +Y+ K+  LE    FV  
Sbjct: 823  XLQMKGLVKKDLCFGFLYSN-DKNFSDKFVLKYEQDXPELLNVYQGKIGILE--LGFVSE 882

Query: 375  PFLLLNKEPTGLSSFSSHFLHSFFSHGFSESPLKSLRYLSNSSEIVSSLKTASLANTAQL 434
               +  +    +S  S      F S    +    ++ YL NS  + S     S +   Q 
Sbjct: 883  GIAIFARRE--MSPGSVCLEXKFRSFSSPKQXSFTVSYLMNSCGL-SPEXALSASRKVQF 942

Query: 435  ENNGKA--VIGLLASHGFSESQISDLAKRYPSLFSANPDKTILPKLLFFQSKGLSSPEIV 494
            E   +A  V+ LL ++G + + IS +  RYP L +ANP+KT+LPKL FF+S G S P++ 
Sbjct: 943  ETPERADSVLALLRNYGCTNTHISKIVSRYPLLLTANPEKTLLPKLEFFRSVGFSGPDLA 1002

Query: 495  KFVCSVPRVLAGSLNKRIIPAFDYIQAVLGSEEKTLAAIKRSADILGWDLQISVGPNIEI 554
              V + P++L  SL   +IP+++++++V+   E  + A+ +S  + G  L   + PNIEI
Sbjct: 1003 SIVAASPQILRRSLENHVIPSYNFLKSVVMVNENIVRALNKSYWLNGQSLPNIIVPNIEI 1062

Query: 555  LKQTGVPDSNISSYLQQQPKMFLTSSIRFKEAVERVTEMGFNPQQMQFVVAVFCLRAMTK 614
            LK  GVP SNIS  +   P     ++++F  +V+ V EMGF+P +++F+ AV  +  M +
Sbjct: 1063 LKDIGVPMSNISFLVTCHPSAVSQNNVKFARSVKMVIEMGFDPLRVKFLKAVQVIVEMAE 1122

Query: 615  STLDKKVEVYRKWGLSEEEIRLAFKKNPWCMMISEDKINGAMDYFVNKIGCQSSYVARRP 674
            S  + K+EVYR+WGL++++I L F+ +P CM  SE KI   MD+ VNK+G + + + R P
Sbjct: 1123 SMWEHKMEVYRRWGLTDDQIMLMFRLDPLCMKSSEKKIMSVMDFLVNKMGWEPAAIGRYP 1182

Query: 675  GLTLYSLKKRLLPRGYIYQVLLSKGLIKKHEYLSSLFNSSENRFIKKFINPHKEQIPGLL 730
             + L SL+K+++P   + +VL  KGL+KK +   S   S E  F  +F+  +++ +P L+
Sbjct: 1183 TVFLRSLEKKIIPWCSVVKVLQMKGLVKK-DLCVSFLGSGEKNFFNRFVVKYEQDVPELV 1227

BLAST of Cucsa.253380 vs. TrEMBL
Match: A5AEZ3_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_028996 PE=4 SV=1)

HSP 1 Score: 418.3 bits (1074), Expect = 1.9e-113
Identity = 246/700 (35.14%), Postives = 399/700 (57.00%), Query Frame = 1

Query: 42   SPKSASLASNVVQLKNNRKA--IIAFFENHGFSESQVSDLIKKVPLILSANPE-ILFPIL 101
            SP+ A  AS  VQ +   +A  ++A   N+G + + +S ++ + PL+L+ANPE  L P L
Sbjct: 915  SPEXALSASRKVQFETPERADSVLALLRNYGCTNTHISKIVSRYPLLLTANPEKTLLPKL 974

Query: 102  LFFKSKGLSSPAITKLVCFAPQILKRSLNQEIIPFFDYIQAVLGTVEKTVATIKRFPRIL 161
             FF+S G S P +  +V  +PQIL+RSL   +IP ++++++V+   E  V  + +   + 
Sbjct: 975  EFFRSVGFSGPDLASIVAASPQILRRSLENHVIPSYNFLKSVVMVNENIVRALNKSYWLN 1034

Query: 162  GWNLRISVGPNIEILKQFGVPDSNISTYLQRQPKVFSTSSIRFKEIVERVTEMGFNPQRL 221
            G +L   + PNIEILK  GVP SNIS  +   P   S ++++F   V+ V EMGF+P R+
Sbjct: 1035 GQSLPNIIVPNIEILKDIGVPMSNISFLVTCHPSAVSQNNVKFARSVKMVIEMGFDPLRV 1094

Query: 222  QFIVAVHALRSLTKSSWDKKLEVYRKWGLSEEEFYLAFRKYPWCMALSEEKINDTMDFFI 281
            +F+ AV  +  + +S W+ K+EVYR+WGL++++  L FR  P CM  SE+KI   MDF +
Sbjct: 1095 KFLKAVQVIVEMAESMWEHKMEVYRRWGLTDDQIMLMFRLDPLCMKSSEKKIMSVMDFLV 1154

Query: 282  NKMGRESSLVARRPSLLSYSLKKRLFPRGYVYQVLLSKGLIKKEKNLDLLFEPPEKRFIE 341
            NKMG E + + R P++   SL+K++ P   V +VL  KGL+KK+  +  L    EK F  
Sbjct: 1155 NKMGWEPAAIGRYPTVFLRSLEKKIIPWCSVVKVLQMKGLVKKDLCVSFLGSG-EKNFFN 1214

Query: 342  KYINAHKEQVPGLLE--LYEQKLMNLEDEQAFVVSPFLLLNKEPTGLSSFSSHFLHSFFS 401
            +++  +++ VP L+          ++        +P++     P G S            
Sbjct: 1215 RFVVKYEQDVPELVNSLCLSNAPCSVPSHMETAATPWIF----PDGSSKAE--------- 1274

Query: 402  HGFSESPLKSLRYLSNSSEIVSSLKTASLANTAQLENNGKAVIGLLASHGFSESQISDLA 461
              F+E  +  L      ++   +L +AS     +      +V+ LL ++G +   IS + 
Sbjct: 1275 --FTECNMLGLLQCXPGNQEKXAL-SASRKIQFETPERADSVLALLRNYGCTNXHISKIV 1334

Query: 462  KRYPSLFSANPDKTILPKLLFFQSKGLSSPEIVKFVCSVPRVLAGSLNKRIIPAFDYIQA 521
             RYP L +ANP+KT+LPKL FF S G S  ++   V + P++L  SL   +IP+++++++
Sbjct: 1335 SRYPLLLTANPEKTLLPKLEFFCSVGFSGXDLASIVVAGPQILKRSLENHVIPSYNFLKS 1394

Query: 522  VLGSEEKTLAAIKRSADILGWDLQISVGPNIEILKQTGVPDSNISSYLQQQPKMFLTSSI 581
            VL   E  + A+ +S  + G  LQ  + PNI IL + GVP SNIS  +   P     +  
Sbjct: 1395 VLMVNENIVRALNKSYWLHGQSLQNIMAPNIAILXEIGVPMSNISFLVTCHPGAVSQNKE 1454

Query: 582  RFKEAVERVTEMGFNPQQMQFVVAVFCLRAMTKSTLDKKVEVYRKWGLSEEEIRLAFKKN 641
            +F  +V+ V EMGF+P ++ FV AV  +  M  S  + K+EVYR+WGL+++EI L F+ +
Sbjct: 1455 KFSRSVKMVIEMGFDPLRVPFVKAVQVIMEMGXSMWEHKMEVYRRWGLTDDEIMLMFRLD 1514

Query: 642  PWCMMISEDKINGAMDYFVNKIGCQSSYVARRPGLTLYSLKKRLLPRGYIYQVLLSKGLI 701
            P CM  SE KI   MD+ VNK+G + + +AR P + L SL+K ++P   + +VL  KGL+
Sbjct: 1515 PLCMKSSEKKIMSVMDFLVNKMGWKPAAIARYPTVFLRSLEKXIIPWCSVVKVLQMKGLV 1574

Query: 702  KKHEYLSSLFNSSENRFIKKFINPHKEQIPGLLELYKERL 737
            KK   LS L ++ +N F  +F+  ++  +P LL +Y+ ++
Sbjct: 1575 KKDLCLSFLGSNEKNXF-NRFMVKYEXDVPELLNVYQGKI 1596

HSP 2 Score: 237.3 bits (604), Expect = 6.1e-59
Identity = 134/357 (37.54%), Postives = 216/357 (60.50%), Query Frame = 1

Query: 6    RRIILPGSPSSVFTHGFSESPLKSLRFLSTSSEIVSSPKSASLASNVVQLKNNRKA--II 65
            R++++  S SS   H F+ S      +L  S  +  SP+SA  AS  VQ +   +A  ++
Sbjct: 2269 RQLLMFRSFSSPKQHSFTVS------YLMNSCGL--SPESALSASRKVQFETPERADSVL 2328

Query: 66   AFFENHGFSESQVSDLIKKVPLILSANPE-ILFPILLFFKSKGLSSPAITKLVCFAPQIL 125
            A   N+G + + +S ++ + P++L+ANPE  L P L FF+S G S P +  +V  +PQIL
Sbjct: 2329 ALLRNYGCTNTHISKIVSRYPVLLTANPEKTLLPKLEFFRSVGFSGPDLASIVAASPQIL 2388

Query: 126  KRSLNQEIIPFFDYIQAVLGTVEKTVATIKRFPRILGWNLRISVGPNIEILKQFGVPDSN 185
            +RSL   +IP ++++++V+   EK V  + +   + G  L+ ++ PNIEILK+ GVP S 
Sbjct: 2389 RRSLENHVIPSYNFLKSVVIVNEKIVRALSKSYWLNGQTLQNTIAPNIEILKEIGVPISK 2448

Query: 186  ISTYLQRQPKVFSTSSIRFKEIVERVTEMGFNPQRLQFIVAVHALRSLTKSSWDKKLEVY 245
            IS ++   P   S +  +F  IV+ VTEMGF+P R++F+ AV  +  + +S W+ K+EVY
Sbjct: 2449 ISFFVTCHPSAVSQNKKKFSRIVKMVTEMGFDPLRVKFVKAVKVICEMGESMWEHKMEVY 2508

Query: 246  RKWGLSEEEFYLAFRKYPWCMALSEEKINDTMDFFINKMGRESSLVARRPSLLSYSLKKR 305
            R WGL++++  L F+  P CMA SE KI   MDF +NKMG E + V R P++   SL+K+
Sbjct: 2509 RGWGLTDDDIMLMFKSDPLCMAASERKIMSVMDFLVNKMGWEHAAVVRYPTVFLCSLEKK 2568

Query: 306  LFPRGYVYQVLLSKGLIKKEKNLDLLFEPPEKRFIEKYINAHKEQVPGLLELYEQKL 360
            + P   V +V+  KGL+KK+  L +L    EK F  +++  +++ V  LL +Y+ K+
Sbjct: 2569 IIPWCSVVKVIQMKGLVKKDLCLCIL-GYSEKNFFNRFVVRYEQDVAELLNVYQGKI 2616

HSP 3 Score: 232.3 bits (591), Expect = 2.0e-57
Identity = 133/364 (36.54%), Postives = 214/364 (58.79%), Query Frame = 1

Query: 375  LLNKEPTGLSSFSSHFLHSFFSHGFSESPLKSLRYLSNSSEIVSSLKTASLANTAQLENN 434
            L N++     SFSS   HSF           ++ YL NS  + S     S +   Q E  
Sbjct: 2266 LQNRQLLMFRSFSSPKQHSF-----------TVSYLMNSCGL-SPESALSASRKVQFETP 2325

Query: 435  GKA--VIGLLASHGFSESQISDLAKRYPSLFSANPDKTILPKLLFFQSKGLSSPEIVKFV 494
             +A  V+ LL ++G + + IS +  RYP L +ANP+KT+LPKL FF+S G S P++   V
Sbjct: 2326 ERADSVLALLRNYGCTNTHISKIVSRYPVLLTANPEKTLLPKLEFFRSVGFSGPDLASIV 2385

Query: 495  CSVPRVLAGSLNKRIIPAFDYIQAVLGSEEKTLAAIKRSADILGWDLQISVGPNIEILKQ 554
             + P++L  SL   +IP+++++++V+   EK + A+ +S  + G  LQ ++ PNIEILK+
Sbjct: 2386 AASPQILRRSLENHVIPSYNFLKSVVIVNEKIVRALSKSYWLNGQTLQNTIAPNIEILKE 2445

Query: 555  TGVPDSNISSYLQQQPKMFLTSSIRFKEAVERVTEMGFNPQQMQFVVAVFCLRAMTKSTL 614
             GVP S IS ++   P     +  +F   V+ VTEMGF+P +++FV AV  +  M +S  
Sbjct: 2446 IGVPISKISFFVTCHPSAVSQNKKKFSRIVKMVTEMGFDPLRVKFVKAVKVICEMGESMW 2505

Query: 615  DKKVEVYRKWGLSEEEIRLAFKKNPWCMMISEDKINGAMDYFVNKIGCQSSYVARRPGLT 674
            + K+EVYR WGL++++I L FK +P CM  SE KI   MD+ VNK+G + + V R P + 
Sbjct: 2506 EHKMEVYRGWGLTDDDIMLMFKSDPLCMAASERKIMSVMDFLVNKMGWEHAAVVRYPTVF 2565

Query: 675  LYSLKKRLLPRGYIYQVLLSKGLIKKHEYLSSLFNSSENRFIKKFINPHKEQIPGLLELY 734
            L SL+K+++P   + +V+  KGL+KK +    +   SE  F  +F+  +++ +  LL +Y
Sbjct: 2566 LCSLEKKIIPWCSVVKVIQMKGLVKK-DLCLCILGYSEKNFFNRFVVRYEQDVAELLNVY 2616

Query: 735  KERL 737
            + ++
Sbjct: 2626 QGKI 2616

HSP 4 Score: 224.9 bits (572), Expect = 3.1e-55
Identity = 128/353 (36.26%), Postives = 207/353 (58.64%), Query Frame = 1

Query: 385 SFSSHFLHSFFSHGFSESPLKSLRYLSNSSEIV-SSLKTASLANTAQLENNGKAVIGLLA 444
           SFSS   HSF           ++ YL NS  +   S  +AS     +  +   +V+ LL 
Sbjct: 40  SFSSSKQHSF-----------TVSYLINSCGLSPESALSASRKVQFETPDGADSVLALLR 99

Query: 445 SHGFSESQISDLAKRYPSLFSANPDKTILPKLLFFQSKGLSSPEIVKFVCSVPRVLAGSL 504
           ++G + + IS +  +YP L  AN +KT+LPKL FF S G S P++V+ V   P +L  SL
Sbjct: 100 NYGCTNTHISKIVSKYPLLLIANSEKTLLPKLEFFXSAGFSGPDLVRIVVGSPSILKRSL 159

Query: 505 NKRIIPAFDYIQAVLGSEEKTLAAIKRSADILGWDLQISVGPNIEILKQTGVPDSNISSY 564
              +IP++++++++    E  + A  RS  + G  +Q ++  N+EILK+ GVP SNISS 
Sbjct: 160 ENHLIPSYNFLKSMDMVHENIVKAFSRSYWLTGKSVQDTIASNVEILKEIGVPMSNISSL 219

Query: 565 LQQQPKMFLTSSIRFKEAVERVTEMGFNPQQMQFVVAVFCLRAMTKSTLDKKVEVYRKWG 624
           +   P     +  +F  +VE+V EMG NP ++ F+ AV  +  + +S  + K++VYR+WG
Sbjct: 220 VAMHPCAVFQNREKFSRSVEKVFEMGINPLRVTFLKAVQVICGVAESMWEHKMQVYRQWG 279

Query: 625 LSEEEIRLAFKKNPWCMMISEDKINGAMDYFVNKIGCQSSYVARRPGLTLYSLKKRLLPR 684
            +++EI L F+ +P C+  SE KI   MD+ VNK+G + + +AR P + L SL+K+++PR
Sbjct: 280 FTDDEIMLMFRLDPLCIKSSEKKIMSVMDFLVNKMGWEPASIARYPTVFLRSLEKKIIPR 339

Query: 685 GYIYQVLLSKGLIKKHEYLSSLFNSSENRFIKKFINPHKEQIPGLLELYKERL 737
             + +VL  KGL+KK   L  L  S EN F  KF+  +++ +P LL +Y+ ++
Sbjct: 340 CSVVKVLQMKGLVKKDLCLGILGCSEEN-FFDKFVVKYEQDVPELLNVYQGKI 380

HSP 5 Score: 224.6 bits (571), Expect = 4.1e-55
Identity = 123/325 (37.85%), Postives = 197/325 (60.62%), Query Frame = 1

Query: 42  SPKSASLASNVVQLK--NNRKAIIAFFENHGFSESQVSDLIKKVPLILSANPE-ILFPIL 101
           SP+SA  AS  VQ +  +   +++A   N+G + + +S ++ K PL+L AN E  L P L
Sbjct: 61  SPESALSASRKVQFETPDGADSVLALLRNYGCTNTHISKIVSKYPLLLIANSEKTLLPKL 120

Query: 102 LFFKSKGLSSPAITKLVCFAPQILKRSLNQEIIPFFDYIQAVLGTVEKTVATIKRFPRIL 161
            FF S G S P + ++V  +P ILKRSL   +IP +++++++    E  V    R   + 
Sbjct: 121 EFFXSAGFSGPDLVRIVVGSPSILKRSLENHLIPSYNFLKSMDMVHENIVKAFSRSYWLT 180

Query: 162 GWNLRISVGPNIEILKQFGVPDSNISTYLQRQPKVFSTSSIRFKEIVERVTEMGFNPQRL 221
           G +++ ++  N+EILK+ GVP SNIS+ +   P     +  +F   VE+V EMG NP R+
Sbjct: 181 GKSVQDTIASNVEILKEIGVPMSNISSLVAMHPCAVFQNREKFSRSVEKVFEMGINPLRV 240

Query: 222 QFIVAVHALRSLTKSSWDKKLEVYRKWGLSEEEFYLAFRKYPWCMALSEEKINDTMDFFI 281
            F+ AV  +  + +S W+ K++VYR+WG +++E  L FR  P C+  SE+KI   MDF +
Sbjct: 241 TFLKAVQVICGVAESMWEHKMQVYRQWGFTDDEIMLMFRLDPLCIKSSEKKIMSVMDFLV 300

Query: 282 NKMGRESSLVARRPSLLSYSLKKRLFPRGYVYQVLLSKGLIKKEKNLDLLFEPPEKRFIE 341
           NKMG E + +AR P++   SL+K++ PR  V +VL  KGL+KK+  L +L    E+ F +
Sbjct: 301 NKMGWEPASIARYPTVFLRSLEKKIIPRCSVVKVLQMKGLVKKDLCLGIL-GCSEENFFD 360

Query: 342 KYINAHKEQVPGLLELYEQKLMNLE 364
           K++  +++ VP LL +Y+ K+  LE
Sbjct: 361 KFVVKYEQDVPELLNVYQGKIGILE 384

HSP 6 Score: 218.8 bits (556), Expect = 2.3e-53
Identity = 120/325 (36.92%), Postives = 191/325 (58.77%), Query Frame = 1

Query: 42   SPKSASLASNVVQLKNNRKA--IIAFFENHGFSESQVSDLIKKVPLILSANPE-ILFPIL 101
            SP+SA  AS  +Q +   +A  ++A   N+G + + +S ++ K PL+L+ANPE  L P L
Sbjct: 1808 SPESALSASRKIQFETPERADSVLALLRNYGCTNTHISKIVSKYPLLLTANPEKTLLPKL 1867

Query: 102  LFFKSKGLSSPAITKLVCFAPQILKRSLNQEIIPFFDYIQAVLGTVEKTVATIKRFPRIL 161
             FF+S G S P +  ++   P ILKRSL   +IP ++++++V    E     ++R   + 
Sbjct: 1868 EFFRSVGFSGPDLAGIIVAKPSILKRSLENHVIPNYNFLKSVGMINENIARALRRTYWLT 1927

Query: 162  GWNLRISVGPNIEILKQFGVPDSNISTYLQRQPKVFSTSSIRFKEIVERVTEMGFNPQRL 221
            G +++ +  PNI  LK+ GVP SNIS +L   P   S +  +F   V++V EMGF+P R+
Sbjct: 1928 GQSVQTTNVPNIATLKEIGVPMSNISFFLTCHPSAVSQNKEKFSTNVKKVIEMGFDPLRV 1987

Query: 222  QFIVAVHALRSLTKSSWDKKLEVYRKWGLSEEEFYLAFRKYPWCMALSEEKINDTMDFFI 281
             F+ AV  +  + +S W+ K+EVYR+WG +++E  L     P CM  SE KI   MDF +
Sbjct: 1988 TFLKAVRLICGMGESMWEHKMEVYRRWGFTDDEIMLMIXLDPLCMTSSERKIMSVMDFLV 2047

Query: 282  NKMGRESSLVARRPSLLSYSLKKRLFPRGYVYQVLLSKGLIKKEKNLDLLFEPPEKRFIE 341
            NKMG E + + R P++   SL+K++ P   V +VL  K L+KK+ +L  L    +K F  
Sbjct: 2048 NKMGWEPAAIGRYPTVFLRSLEKKIIPWCSVVKVLQIKXLVKKDLSLSFL-GSSKKNFFN 2107

Query: 342  KYINAHKEQVPGLLELYEQKLMNLE 364
            +++  ++  VP LL +Y+ K+  LE
Sbjct: 2108 RFVVKYEHDVPELLNVYQGKIGILE 2131

HSP 7 Score: 212.6 bits (540), Expect = 1.6e-51
Identity = 123/347 (35.45%), Postives = 200/347 (57.64%), Query Frame = 1

Query: 393  SFFS---HGFSESPLKSLRYLSNSSEIVSSLKTASLANTAQLENNGKAVIGLLASHGFSE 452
            SFFS   H F+ S L +   LS  S + +S K        +      +V+ LL ++G + 
Sbjct: 1787 SFFSPKQHSFTVSYLMNSCGLSPESALSASRKIQF-----ETPERADSVLALLRNYGCTN 1846

Query: 453  SQISDLAKRYPSLFSANPDKTILPKLLFFQSKGLSSPEIVKFVCSVPRVLAGSLNKRIIP 512
            + IS +  +YP L +ANP+KT+LPKL FF+S G S P++   + + P +L  SL   +IP
Sbjct: 1847 THISKIVSKYPLLLTANPEKTLLPKLEFFRSVGFSGPDLAGIIVAKPSILKRSLENHVIP 1906

Query: 513  AFDYIQAVLGSEEKTLAAIKRSADILGWDLQISVGPNIEILKQTGVPDSNISSYLQQQPK 572
             ++++++V    E    A++R+  + G  +Q +  PNI  LK+ GVP SNIS +L   P 
Sbjct: 1907 NYNFLKSVGMINENIARALRRTYWLTGQSVQTTNVPNIATLKEIGVPMSNISFFLTCHPS 1966

Query: 573  MFLTSSIRFKEAVERVTEMGFNPQQMQFVVAVFCLRAMTKSTLDKKVEVYRKWGLSEEEI 632
                +  +F   V++V EMGF+P ++ F+ AV  +  M +S  + K+EVYR+WG +++EI
Sbjct: 1967 AVSQNKEKFSTNVKKVIEMGFDPLRVTFLKAVRLICGMGESMWEHKMEVYRRWGFTDDEI 2026

Query: 633  RLAFKKNPWCMMISEDKINGAMDYFVNKIGCQSSYVARRPGLTLYSLKKRLLPRGYIYQV 692
             L    +P CM  SE KI   MD+ VNK+G + + + R P + L SL+K+++P   + +V
Sbjct: 2027 MLMIXLDPLCMTSSERKIMSVMDFLVNKMGWEPAAIGRYPTVFLRSLEKKIIPWCSVVKV 2086

Query: 693  LLSKGLIKKHEYLSSLFNSSENRFIKKFINPHKEQIPGLLELYKERL 737
            L  K L+KK   LS L  SS+  F  +F+  ++  +P LL +Y+ ++
Sbjct: 2087 LQIKXLVKKDLSLSFL-GSSKKNFFNRFVVKYEHDVPELLNVYQGKI 2127

HSP 8 Score: 425.6 bits (1093), Expect = 1.2e-115
Identity = 242/701 (34.52%), Postives = 416/701 (59.34%), Query Frame = 1

Query: 44   KSASLASNVVQLKNNR--KAIIAFFENHGFSESQVSDLIKKVPLILSANPE-ILFPILLF 103
            K+A+ AS +V  +++    +++AF +NHGFS+ Q++ LI ++P ++ ++PE  L P + F
Sbjct: 878  KTATSASQMVHFESSEGPDSVLAFLKNHGFSDIQIAKLITRLPRLVCSDPEETLLPKIEF 937

Query: 104  FKSKGLSSPAITKLVCFAPQILKRSLNQEIIPFFDYIQAVLGTVEKTVATIKRFPRILGW 163
            F S G+S    T++V   P I  RS+ +  +P +D+I++++ + +K V T+KR PR+L  
Sbjct: 938  FSSIGISGHDFTRIVTQNPNIWFRSVEKRFVPCYDFIKSMVLSEDKVVTTLKRAPRMLMC 997

Query: 164  NLRISVGPNIEILKQFGVPDSNISTYLQRQPKVFSTSSIRFKEIVERVTEMGFNPQRLQF 223
            +++ S+ PNI  L++FGV  S +   +   P +   +S +F++ V  V +MGF+P++ +F
Sbjct: 998  DMQTSIAPNIASLRKFGVTQSTVLFLVTDYPNILLRTSAKFEQHVREVVDMGFDPKKSEF 1057

Query: 224  IVAVHALRSLTKSSWDKKLEVYRKWGLSEEEFYLAFRKYPWCMALSEEKINDTMDFFINK 283
            + A+     +++ S ++K+ +YR +G SEEE     + +P C+ LSE+KI D +DF +NK
Sbjct: 1058 VHALRVFAGMSELSRERKMAIYRWFGWSEEEILSVLKTHPMCLILSEKKIMDGLDFLMNK 1117

Query: 284  MGRESSLVARRPSLLSYSLKKRLFPRGYVYQVLLSKGLIKK-EKNLDLLFEPPEKRFIEK 343
            MG +   VAR P +L YSL KR+ PR  V QVL SKGL+K+ +  L  +  PPEK  +  
Sbjct: 1118 MGWQREAVARVPLVLCYSLNKRVIPRCSVXQVLQSKGLLKEADFYLSSVLIPPEKVILAS 1177

Query: 344  YINAHKEQVPGLLELYEQKLMNLEDEQAFVVSPFLLLNKEPTGLSSFSSHFLHSFFSHGF 403
            +          L         N+++   F+ +  L+L +  +  S+  S+       H F
Sbjct: 1178 F----------LCRTLRFSAPNIQNSSCFLENVSLVLIRGLSSSSNKISNAADQQQQHSF 1237

Query: 404  SESPLKSLRYLSNSSEIVSSLKTASLANTA---QLENNGKAVIGLLASHGFSESQISDLA 463
            + S      YL N   +   LKTA+ A+     +      +V+  L +HGFS++QI+ L 
Sbjct: 1238 TVS------YLVNKCGL--PLKTATSASQMVHFESSEGPDSVLAFLKNHGFSDTQIAKLI 1297

Query: 464  KRYPSLFSANPDKTILPKLLFFQSKGLSSPEIVKFVCSVPRVLAGSLNKRIIPAFDYIQA 523
             R P L  ++P++T+LPK+ FF S G+  P+  + +   P +   S+ KR+ P +D+I++
Sbjct: 1298 TRRPRLVCSDPEETLLPKIEFFNSIGIRGPDFTRILTQNPNIWFRSVKKRLAPCYDFIRS 1357

Query: 524  VLGSEEKTLAAIKRSADILGWDLQISVGPNIEILKQTGVPDSNISSYLQQQPKMFLTSSI 583
            V+ SE+K +  +K +  +L  D+Q S+ PNI +L++ GV  S +   +   P + L +S 
Sbjct: 1358 VVLSEDKAVTTLKGAPRMLMCDMQTSIAPNIALLRKFGVSQSTLLFLVTGFPNLLLRTSA 1417

Query: 584  RFKEAVERVTEMGFNPQQMQFVVAVFCLRAMTKSTLDKKVEVYRKWGLSEEEIRLAFKKN 643
            +F++ V  V +MGF+P++ +FV A+     ++K + ++K+ VY ++G S+ EI    K +
Sbjct: 1418 KFEKHVREVLDMGFDPKKSEFVHALRVFAGISKLSRERKMAVYSRFGWSDHEILSVLKTH 1477

Query: 644  PWCMMISEDKINGAMDYFVNKIGCQSSYVARRPGLTLYSLKKRLLPRGYIYQVLLSKGLI 703
            P C+M+SE KI   +D+ +NK+G Q   VAR P +  YSL KR++PR  + QVL S+GL+
Sbjct: 1478 PMCLMLSEKKIMDGLDFLMNKMGWQRKAVARVPLVLCYSLNKRVIPRCAVVQVLQSEGLL 1537

Query: 704  KKHE-YLSSLFNSSENRFIKKFINPHKEQIPGLLELYKERL 737
            K+ + YLSS+   SE  F+ +F+  ++EQ+P LL +YK +L
Sbjct: 1538 KEADFYLSSVLIPSEKVFLARFVIKYEEQVPQLLNVYKGKL 1560

BLAST of Cucsa.253380 vs. TrEMBL
Match: A5AEZ1_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_028994 PE=4 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 8.0e-51
Identity = 126/381 (33.07%), Postives = 211/381 (55.38%), Query Frame = 1

Query: 342  NAHKEQVPGLLELYEQKLMNLEDEQAFVVSPFLLLNKEPTGLSSFSSHFLHSFFSHGFSE 401
            N  K   P       +   N+++   F  +  L+L +  +  S+  S        H F+ 
Sbjct: 807  NWEKYAAPFEYRTLRRSAPNIQNSSCFRENVSLVLVRGLSSSSNKISKAADQQKQHSFTV 866

Query: 402  SPLKSLRYLSNSSEIVSSLKTASLANTA---QLENNGKAVIGLLASHGFSESQISDLAKR 461
            S      YL N   +   LKTA+ A+     +      +V+  L +HGFS+ QI+ L  R
Sbjct: 867  S------YLVNRCGL--PLKTATSASQMVHFESSEGPDSVLAFLKNHGFSDIQIAKLITR 926

Query: 462  YPSLFSANPDKTILPKLLFFQSKGLSSPEIVKFVCSVPRVLAGSLNKRIIPAFDYIQAVL 521
             P L  ++P++T+LPK+ FF S G+S  +  + V   P +   S+ KR +P +D+I++++
Sbjct: 927  LPRLVCSDPEETLLPKIEFFSSIGISGHDFTRIVTQNPNIWFRSVEKRFVPCYDFIKSMV 986

Query: 522  GSEEKTLAAIKRSADILGWDLQISVGPNIEILKQTGVPDSNISSYLQQQPKMFLTSSIRF 581
             SE+K +  +KR+  +L  D+Q S+ PNI  L++ GV  S +   +   P + L +S +F
Sbjct: 987  LSEDKVVTTLKRAPRMLMCDMQTSIAPNIASLRKFGVTQSTVLFLVTDYPNILLRTSAKF 1046

Query: 582  KEAVERVTEMGFNPQQMQFVVAVFCLRAMTKSTLDKKVEVYRKWGLSEEEIRLAFKKNPW 641
            ++ V  V +MGF+P++ +FV A+     M++ + ++K+ +YR +G SEEEI    K +P 
Sbjct: 1047 EQHVREVVDMGFDPKKSEFVHALRVFAGMSELSRERKMAIYRWFGWSEEEILSVLKTHPM 1106

Query: 642  CMMISEDKINGAMDYFVNKIGCQSSYVARRPGLTLYSLKKRLLPRGYIYQVLLSKGLIKK 701
            C+++SE KI   +D+ +NK+G Q   VAR P +  YSL KR++PR  + QVL SKGL+K+
Sbjct: 1107 CLILSEKKIMDGLDFLMNKMGWQREAVARVPLVLCYSLNKRVIPRCSVXQVLQSKGLLKE 1166

Query: 702  HE-YLSSLFNSSENRFIKKFI 719
             + YLSS+    E   +  F+
Sbjct: 1167 ADFYLSSVLIPPEKVILASFL 1179

HSP 2 Score: 422.5 bits (1085), Expect = 1.0e-114
Identity = 252/740 (34.05%), Postives = 413/740 (55.81%), Query Frame = 1

Query: 13   SPSSVFTHGFSESPLKSLRFLSTSSEIVSSPKSASLASNVVQLKN--NRKAIIAFFENHG 72
            +P+      FS S   S       +    SP++A  AS  +  +N  N  +I+A   N G
Sbjct: 398  NPTPFIIRSFSASNQHSFTVSYLVNSCGLSPETAISASGKIHFENPKNPDSILALLRNSG 457

Query: 73   FSESQVSDLIKKVPLILSANPE-ILFPILLFFKSKGLSSPAITKLVCFAPQILKRSLNQE 132
             + + ++ ++ K+P +L  NPE  L P L FF S GLS   +  ++   P +L RSL   
Sbjct: 458  CTNTHITKIVTKLPSLLLVNPEKTLLPKLDFFGSMGLSGARLASILSSEPIVLMRSLENA 517

Query: 133  IIPFFDYIQAVLGTVEKTVATIKRFPRILGWNLRISVGPNIEILKQFGVPDSNISTYLQR 192
            +IP +++++++  + E  +  +K    I   NL   +  NI ++++ GVP S+IS  + R
Sbjct: 518  LIPKYNFLKSLQISNEDAIKILKSSCWISCGNLERIIATNIAVMREIGVPISHISVLVAR 577

Query: 193  QPKVFSTSSIRFKEIVERVTEMGFNPQRLQFIVAVHALRSLTKSSWDKKLEVYRKWGLSE 252
               +   S  +F E V++V EMGFNP +  F+ A+ A+   T+S+W +K+E+YR+WG SE
Sbjct: 578  YHTICQRSD-KFSENVKKVVEMGFNPLKFAFVNALQAVCQTTESTWQQKMEMYRRWGWSE 637

Query: 253  EEFYLAFRKYPWCMALSEEKINDTMDFFINKMGRESSLVARRPSLLSYSLKKRLFPRGYV 312
            +E   AFR  P CM LSE+K+   +DF +NKMG   ++VAR P  +  + +KR+ PR  V
Sbjct: 638  DEILSAFRNRPQCMQLSEKKVTKVLDFLVNKMGWRPAVVARAPIAICLNFEKRVAPRCSV 697

Query: 313  YQVLLSKGLIKKEKNLDLLFEPPEKRFIEKYINAHKEQVPGLLELYEQKLMNLED----- 372
             +VL  KGLIKK+  L      PE  F++KY+  +++++P LL++Y+ K+          
Sbjct: 698  VKVLSLKGLIKKDLKLGTFLNLPEGDFLDKYVIKYQDEIPQLLDVYQGKVEMCSIFLCGR 757

Query: 373  ----------EQAFVVSPFLLLNKEPTGLSSFSSHFLHSFFSHGFSESPLKSLRYLSNSS 432
                        A +     L N  P  +  FSS   HSF           ++ YL NS 
Sbjct: 758  TGLPPSWRRIGGAAMTQLHFLGNITPFVIRCFSSSKQHSF-----------TVSYLMNSC 817

Query: 433  EIVSSLKTASLANTAQLEN--NGKAVIGLLASHGFSESQISDLAKRYPSLFSANPDKTIL 492
             + S     S +   Q EN  N  +V+ LL +HG +++ IS +  ++P L  ANP+KT+L
Sbjct: 818  GL-SPETAISTSKKVQFENPENPDSVLALLRNHGCTDTHISKIVSKHPLLLLANPEKTLL 877

Query: 493  PKLLFFQSKGLSSPEIVKFVCSVPRVLAGSLNKRIIPAFDYIQAVLGSEEKTLAAIKRSA 552
            PKL F  S GLS  ++ K + S P +L  SL K +IP ++ ++ V+  +E  + A+ +  
Sbjct: 878  PKLQFLGSVGLSHVDLAKVLASTPSILRMSLEKTLIPTYNLLKGVVIGDENAVKALTKQC 937

Query: 553  DILGWDLQISVGPNIEILKQTGVPDSNISSYLQQQPKMFLTSSIRFKEAVERVTEMGFNP 612
             I   +++ ++ PN  +L++ GVP ++IS  +   P +      +F + V++V EMGFNP
Sbjct: 938  RICCGNVEKTIAPNATLLREIGVPMAHISFLVTNYPTLCQKRD-KFSKTVKKVMEMGFNP 997

Query: 613  QQMQFVVAVFCLRAMTKSTLDKKVEVYRKWGLSEEEIRLAFKKNPWCMMISEDKINGAMD 672
            Q++ FV A+  +  M++ST ++K+  Y++ GLSE+EI LAF+ +P C  +SE KI   MD
Sbjct: 998  QRLLFVNALQVICQMSESTWEQKINAYKRCGLSEDEIVLAFRNHPICFQLSEKKIMSTMD 1057

Query: 673  YFVNKIGCQSSYVARRPGLTLYSLKKRLLPRGYIYQVLLSKGLIKKHEYLSSLFNSSENR 732
            Y VN +G Q   +AR P +  ++L++R++PR  + +VLL KGL+KK   L +    +E  
Sbjct: 1058 YIVN-MGWQPGTIARVPAVLFFNLERRIVPRCSVAKVLLLKGLVKKDLCLGTFLKLTERA 1117

BLAST of Cucsa.253380 vs. TAIR10
Match: AT5G07900.1 (AT5G07900.1 Mitochondrial transcription termination factor family protein)

HSP 1 Score: 231.1 bits (588), Expect = 2.2e-60
Identity = 124/318 (38.99%), Postives = 199/318 (62.58%), Query Frame = 1

Query: 42  SPKSASLASNVVQLKNNRK--AIIAFFENHGFSESQVSDLIKKVPLILSANPE-ILFPIL 101
           SP SA++AS  + L +  +   ++    +HGF+ +Q+S L+KK P++L AN E +L P L
Sbjct: 82  SPDSATVASRKLLLDSPERPNTVLNLLRDHGFTTAQISSLVKKRPVLLLANAESVLLPKL 141

Query: 102 LFFKSKGLSSPAITKLVCFAPQILKRSLNQEIIPFFDYIQAVLGTVEKTVATIKRFPRIL 161
            FF S G+S   + + +   P IL RSL  ++IP ++++++VL + EK VA ++R   + 
Sbjct: 142 SFFLSIGVSKSLLARTLASDPTILTRSLVNQLIPSYNFLKSVLDSDEKIVAALRRTTWVF 201

Query: 162 GWNLRISVGPNIEILKQFGVPDSNISTYLQRQPKVFSTSSIRFKEIVERVTEMGFNPQRL 221
             +   ++ PNI  + + GVP+  I   L   P+     +  F+ I ++  EMGFNPQ+ 
Sbjct: 202 LEDHTKNLVPNINYMAETGVPEKCIKLLLTHFPEAVMQKNHEFQAIAKQAQEMGFNPQKS 261

Query: 222 QFIVAVHALRSL-TKSSWDKKLEVYRKWGLSEEEFYLAFRKYPWCMALSEEKINDTMDFF 281
            F++A+HAL     KS WDK  EVY++WG SE++   AF+K+P CM LSE KIN TM++F
Sbjct: 262 TFVLAIHALSGKGNKSIWDKCFEVYQRWGWSEDDIMCAFKKHPHCMMLSERKINRTMEYF 321

Query: 282 INKMGRESSLVARRPSLLSYSLKKRLFPRGYVYQVLLSKGLIKKEKNLDLLFEPPEKRFI 341
           +N+M      +A+ P +L +SL+KR+ PR  V +VL+S GL+K++ +L  L  P EK F+
Sbjct: 322 VNEMNMAPRSIAQCPVVLFFSLEKRIIPRCSVTKVLVSNGLVKEDWSLTSLLVPVEKVFL 381

Query: 342 EKYINAHKEQVPGLLELY 356
           EK +  ++E++P L+ LY
Sbjct: 382 EKLVIKYEEELPELMNLY 399

HSP 2 Score: 223.8 bits (569), Expect = 3.5e-58
Identity = 125/324 (38.58%), Postives = 199/324 (61.42%), Query Frame = 1

Query: 410 LSNSSEIVSSLKTASLANTAQLENNGKAVIGLLASHGFSESQISDLAKRYPSLFSANPDK 469
           LS  S  V+S K   L ++ +  N    V+ LL  HGF+ +QIS L K+ P L  AN + 
Sbjct: 81  LSPDSATVASRKL--LLDSPERPNT---VLNLLRDHGFTTAQISSLVKKRPVLLLANAES 140

Query: 470 TILPKLLFFQSKGLSSPEIVKFVCSVPRVLAGSLNKRIIPAFDYIQAVLGSEEKTLAAIK 529
            +LPKL FF S G+S   + + + S P +L  SL  ++IP+++++++VL S+EK +AA++
Sbjct: 141 VLLPKLSFFLSIGVSKSLLARTLASDPTILTRSLVNQLIPSYNFLKSVLDSDEKIVAALR 200

Query: 530 RSADILGWDLQISVGPNIEILKQTGVPDSNISSYLQQQPKMFLTSSIRFKEAVERVTEMG 589
           R+  +   D   ++ PNI  + +TGVP+  I   L   P+  +  +  F+   ++  EMG
Sbjct: 201 RTTWVFLEDHTKNLVPNINYMAETGVPEKCIKLLLTHFPEAVMQKNHEFQAIAKQAQEMG 260

Query: 590 FNPQQMQFVVAVFCLRAM-TKSTLDKKVEVYRKWGLSEEEIRLAFKKNPWCMMISEDKIN 649
           FNPQ+  FV+A+  L     KS  DK  EVY++WG SE++I  AFKK+P CMM+SE KIN
Sbjct: 261 FNPQKSTFVLAIHALSGKGNKSIWDKCFEVYQRWGWSEDDIMCAFKKHPHCMMLSERKIN 320

Query: 650 GAMDYFVNKIGCQSSYVARRPGLTLYSLKKRLLPRGYIYQVLLSKGLIKKHEYLSSLFNS 709
             M+YFVN++      +A+ P +  +SL+KR++PR  + +VL+S GL+K+   L+SL   
Sbjct: 321 RTMEYFVNEMNMAPRSIAQCPVVLFFSLEKRIIPRCSVTKVLVSNGLVKEDWSLTSLLVP 380

Query: 710 SENRFIKKFINPHKEQIPGLLELY 733
            E  F++K +  ++E++P L+ LY
Sbjct: 381 VEKVFLEKLVIKYEEELPELMNLY 399

BLAST of Cucsa.253380 vs. TAIR10
Match: AT1G21150.1 (AT1G21150.1 Mitochondrial transcription termination factor family protein)

HSP 1 Score: 198.0 bits (502), Expect = 2.1e-50
Identity = 102/316 (32.28%), Postives = 194/316 (61.39%), Query Frame = 1

Query: 42  SPKSASLASNVVQLKNNRK--AIIAFFENHGFSESQVSDLIKKVPLILSANPE-ILFPIL 101
           S +SA   S  V+L +++K  +++A F++HGF+  Q++ +IK  P +LS +PE ++ P L
Sbjct: 69  SLESAKSNSRFVKLVSSKKPDSVLALFKDHGFTNDQITSVIKSFPRVLSLSPEDVISPKL 128

Query: 102 LFFKSKGLSSPAITKLVCFAPQILKRSLNQEIIPFFDYIQAVLGTVEKTVATIKRFPRIL 161
           +FF S G S+    K++  +P++L  SL++ +IP +D ++++L   E  V  +KR  R  
Sbjct: 129 MFFSSIGFSTSDTAKMISSSPKMLSYSLHKRLIPCYDSLKSILVEEESVVKCLKRGIRCF 188

Query: 162 GWNLRISVGPNIEILKQFGVPDSNISTYLQRQPKVFSTSSIRFKEIVERVTEMGFNPQRL 221
              +   V   + I ++ GVPD +I   +Q  P  F +   RF E++ RV   GF+P++ 
Sbjct: 189 SLKITHCVSLRVSICRELGVPDKSIKWLVQASPFTFFSRERRFNEVLNRVCSYGFDPKKA 248

Query: 222 QFIVAVHALRSLTKSSWDKKLEVYRKWGLSEEEFYLAFRKYPWCMALSEEKINDTMDFFI 281
            F+ A+ A    ++S+ ++K ++++ +G S+E+F  A  ++P C+ +S+EKI  T+++ +
Sbjct: 249 GFVHAMVAFDCTSESAMERKFKLFQCFGWSKEDFVAAIMRFPNCVTVSDEKIMYTLEYLV 308

Query: 282 NKMGRESSLVARRPSLLSYSLKKRLFPRGYVYQVLLSKGLIKKEK-NLDLLFEPPEKRFI 341
           N +G ++  +  RP +LS S++KR+ PR  V  +LLSKGL+KKE  N   + +     F+
Sbjct: 309 NNIGLQARDIVARPVVLSLSMEKRIKPRNQVISLLLSKGLVKKEDINYFTILKLKSSEFM 368

Query: 342 EKYINAHKEQVPGLLE 354
           +K++  +++++P L++
Sbjct: 369 DKFVLKYQDEMPQLVQ 384

HSP 2 Score: 191.8 bits (486), Expect = 1.5e-48
Identity = 110/356 (30.90%), Postives = 195/356 (54.78%), Query Frame = 1

Query: 376 LNKEPTGLSSFSSHFLHSFFSHGFSESPLKSLRYLSNSSEIVSSLKTASLANTAQLENNG 435
           L K+   L SF+  +L    S G S   L+S +  S   ++VSS K              
Sbjct: 47  LEKQKPCLESFTVSYLVD--SCGLS---LESAKSNSRFVKLVSSKKP------------- 106

Query: 436 KAVIGLLASHGFSESQISDLAKRYPSLFSANPDKTILPKLLFFQSKGLSSPEIVKFVCSV 495
            +V+ L   HGF+  QI+ + K +P + S +P+  I PKL+FF S G S+ +  K + S 
Sbjct: 107 DSVLALFKDHGFTNDQITSVIKSFPRVLSLSPEDVISPKLMFFSSIGFSTSDTAKMISSS 166

Query: 496 PRVLAGSLNKRIIPAFDYIQAVLGSEEKTLAAIKRSADILGWDLQISVGPNIEILKQTGV 555
           P++L+ SL+KR+IP +D ++++L  EE  +  +KR        +   V   + I ++ GV
Sbjct: 167 PKMLSYSLHKRLIPCYDSLKSILVEEESVVKCLKRGIRCFSLKITHCVSLRVSICRELGV 226

Query: 556 PDSNISSYLQQQPKMFLTSSIRFKEAVERVTEMGFNPQQMQFVVAVFCLRAMTKSTLDKK 615
           PD +I   +Q  P  F +   RF E + RV   GF+P++  FV A+      ++S +++K
Sbjct: 227 PDKSIKWLVQASPFTFFSRERRFNEVLNRVCSYGFDPKKAGFVHAMVAFDCTSESAMERK 286

Query: 616 VEVYRKWGLSEEEIRLAFKKNPWCMMISEDKINGAMDYFVNKIGCQSSYVARRPGLTLYS 675
            ++++ +G S+E+   A  + P C+ +S++KI   ++Y VN IG Q+  +  RP +   S
Sbjct: 287 FKLFQCFGWSKEDFVAAIMRFPNCVTVSDEKIMYTLEYLVNNIGLQARDIVARPVVLSLS 346

Query: 676 LKKRLLPRGYIYQVLLSKGLIKKHEY-LSSLFNSSENRFIKKFINPHKEQIPGLLE 731
           ++KR+ PR  +  +LLSKGL+KK +    ++     + F+ KF+  +++++P L++
Sbjct: 347 MEKRIKPRNQVISLLLSKGLVKKEDINYFTILKLKSSEFMDKFVLKYQDEMPQLVQ 384

BLAST of Cucsa.253380 vs. TAIR10
Match: AT5G64950.1 (AT5G64950.1 Mitochondrial transcription termination factor family protein)

HSP 1 Score: 154.1 bits (388), Expect = 3.4e-37
Identity = 89/305 (29.18%), Postives = 170/305 (55.74%), Query Frame = 1

Query: 436 KAVIGLLASHGFSESQISDLAKRYPSLFSANPDKTILPKLLFFQSKGLSSPEIVKFVCSV 495
           ++VI +L S+ FS++QI    + +P +   N +K + PKL FF+  G +   + KFV   
Sbjct: 72  RSVIQMLKSYSFSDTQIQKSIRVHPRMMFYNVEKILEPKLRFFKDIGFTGSGLGKFVSQN 131

Query: 496 PRVLAGSLNKRIIPAFDYIQAVLGSEEKTLAAIKRSAD--ILGWDLQISVGPNIEILKQT 555
             V+  SL K++IP  + +++++  + + L  I       +L  D  + + PNI  L+  
Sbjct: 132 SSVVGVSLVKKLIPTVEILKSIVAPKHEDLPVILSRCGWLLLSRDPNLFLLPNISYLETC 191

Query: 556 GVPDSNISSYLQQQPKMFLTSSIRFKEAVERVTEMGFNPQQMQFVVAVFCLRAMTKSTLD 615
           G+  S ++S L++QP++F  S  + +  V R  ++GF       V AV  L ++++ T D
Sbjct: 192 GIVGSQLASLLRRQPRIFNLSEEKLRGYVSRALDLGFTLNSRMLVHAVISLSSLSEKTFD 251

Query: 616 KKVEVYRKWGLSEEEIRLAFKKNPWCMMISEDKINGAMDYFVNKIGCQSSYVARRPGLTL 675
           +KV+++   G SE+EI    +++P  +  SEDK+    ++++ ++G +   +A+RP +  
Sbjct: 252 RKVKLFMANGFSEDEITDIIRRSPGLIRCSEDKLTLGFEFYLKRMGLEREALAKRPCVLS 311

Query: 676 YSLKKRLLPRGYIYQVLLSKGLI----KKHEYLSSLFNSSENRFIKKFINPHKEQI-PGL 734
           Y+L+KR++PR  + Q+L  KGL+    KK + +  +   +E  F++K++    ++I   L
Sbjct: 312 YNLEKRVIPRLKVLQILREKGLLLKEEKKKKNMVGIVEMTEEAFLEKYVVRFGDEIAEEL 371

HSP 2 Score: 151.0 bits (380), Expect = 2.9e-36
Identity = 99/335 (29.55%), Postives = 183/335 (54.63%), Query Frame = 1

Query: 43  PKSASLASNVVQLKN--NRKAIIAFFENHGFSESQVSDLIKKVPLILSANPE-ILFPILL 102
           P + ++A     LK+    +++I   +++ FS++Q+   I+  P ++  N E IL P L 
Sbjct: 53  PLAIAIAMRYPNLKSLEQPRSVIQMLKSYSFSDTQIQKSIRVHPRMMFYNVEKILEPKLR 112

Query: 103 FFKSKGLSSPAITKLVCFAPQILKRSLNQEIIPFFDYIQAVLGTVEKTVATIKRFPRILG 162
           FFK  G +   + K V     ++  SL +++IP  + +++++    + +  I       G
Sbjct: 113 FFKDIGFTGSGLGKFVSQNSSVVGVSLVKKLIPTVEILKSIVAPKHEDLPVILSR---CG 172

Query: 163 WNL-----RISVGPNIEILKQFGVPDSNISTYLQRQPKVFSTSSIRFKEIVERVTEMGFN 222
           W L      + + PNI  L+  G+  S +++ L+RQP++F+ S  + +  V R  ++GF 
Sbjct: 173 WLLLSRDPNLFLLPNISYLETCGIVGSQLASLLRRQPRIFNLSEEKLRGYVSRALDLGFT 232

Query: 223 PQRLQFIVAVHALRSLTKSSWDKKLEVYRKWGLSEEEFYLAFRKYPWCMALSEEKINDTM 282
                 + AV +L SL++ ++D+K++++   G SE+E     R+ P  +  SE+K+    
Sbjct: 233 LNSRMLVHAVISLSSLSEKTFDRKVKLFMANGFSEDEITDIIRRSPGLIRCSEDKLTLGF 292

Query: 283 DFFINKMGRESSLVARRPSLLSYSLKKRLFPRGYVYQVLLSKGLI----KKEKNLDLLFE 342
           +F++ +MG E   +A+RP +LSY+L+KR+ PR  V Q+L  KGL+    KK+KN+  + E
Sbjct: 293 EFYLKRMGLEREALAKRPCVLSYNLEKRVIPRLKVLQILREKGLLLKEEKKKKNMVGIVE 352

Query: 343 PPEKRFIEKYINAHKEQVPGLLELYEQKLMNLEDE 366
             E+ F+EKY+    +++   L L   K + L DE
Sbjct: 353 MTEEAFLEKYVVRFGDEIAEEL-LVAYKCVCLVDE 383

HSP 3 Score: 53.1 bits (126), Expect = 8.3e-07
Identity = 76/347 (21.90%), Postives = 146/347 (42.07%), Query Frame = 1

Query: 197 SSIRFKEIVERVTEMGFN-PQRLQFIVAVHALRSLTKSSWDKKLEVYRKWGLSEEEFYLA 256
           +SI     VE + + GF  P  +   +    L+SL +      +++ + +  S+ +   +
Sbjct: 34  ASISNPAFVEFLRDNGFQKPLAIAIAMRYPNLKSLEQPR--SVIQMLKSYSFSDTQIQKS 93

Query: 257 FRKYPWCMALSEEKINDTMDFFINKMGRESS----LVARRPSLLSYSLKKRLFPRGYVYQ 316
            R +P  M  + EKI +    F   +G   S     V++  S++  SL K+L P   + +
Sbjct: 94  IRVHPRMMFYNVEKILEPKLRFFKDIGFTGSGLGKFVSQNSSVVGVSLVKKLIPTVEILK 153

Query: 317 VLLSKGLIKKEKNLDLLFEPPEKRFIEKYINAHKEQVPGLLELYEQKLMNLEDEQAFVVS 376
            +++     K ++L ++        + +  N     +P +  L          E   +V 
Sbjct: 154 SIVAP----KHEDLPVILSRCGWLLLSRDPNLFL--LPNISYL----------ETCGIVG 213

Query: 377 PFL--LLNKEPTGLSSFSSHFLHSFFSHGFSESPLKSLRYLSNSSEIVSSLKTASLANTA 436
             L  LL ++P  + + S   L  + S          L +  NS  +V ++ + S  +  
Sbjct: 214 SQLASLLRRQPR-IFNLSEEKLRGYVSRALD------LGFTLNSRMLVHAVISLSSLSEK 273

Query: 437 QLENNGKAVIGLLASHGFSESQISDLAKRYPSLFSANPDKTILPKLLFFQSKGLSSPEIV 496
             +   K    L  ++GFSE +I+D+ +R P L   + DK  L    + +  GL    + 
Sbjct: 274 TFDRKVK----LFMANGFSEDEITDIIRRSPGLIRCSEDKLTLGFEFYLKRMGLEREALA 333

Query: 497 KFVCSVPRVLAGSLNKRIIPAFDYIQAVLGSEEKTLAAIKRSADILG 537
           K  C    VL+ +L KR+IP    +Q +L  +   L   K+  +++G
Sbjct: 334 KRPC----VLSYNLEKRVIPRLKVLQ-ILREKGLLLKEEKKKKNMVG 346

BLAST of Cucsa.253380 vs. TAIR10
Match: AT5G23930.1 (AT5G23930.1 Mitochondrial transcription termination factor family protein)

HSP 1 Score: 127.1 bits (318), Expect = 4.5e-29
Identity = 75/243 (30.86%), Postives = 133/243 (54.73%), Query Frame = 1

Query: 406 SLRYLSNSSEIVSSL-KTASLANTAQLENNGKAVIGLLASHGFSESQISDLAKRYPSLFS 465
           ++ YL +S  + + L ++ S+      + N  +V+ LL S+GF +SQIS +   YP    
Sbjct: 57  TVSYLIDSLGLTTKLAESISMKANFDEKGNPDSVLKLLRSYGFKDSQISSIISTYPRFLI 116

Query: 466 ANPDKTILPKLLFFQSKGLSSPEIVKFVCSVPRVLAGSLNKRIIPAFDYIQAVLGSEEKT 525
            NP+KT+  KL F +  G SS E+ + V  VP++L     K I   +DY++ +L  ++ +
Sbjct: 117 ENPEKTLRAKLHFLKLNGASSSELTEIVSKVPKILGKRGGKWISHYYDYVKEILQDQDSS 176

Query: 526 LAAIKRSADILGWDLQISVGPNIEILKQTGVPDSNISSYLQQQPKMFLTSSIRFKEAVER 585
            ++ KR         Q +   N+ +L++ GVP   + + L  + K  +    RF+E+V++
Sbjct: 177 SSSSKRK--------QTNRNRNVSVLRKLGVPQRLLLNLLISRAKP-VCGKERFEESVKK 236

Query: 586 VTEMGFNPQQMQFVVAVFCLRAMTKSTLDKKVEVYRKWGLSEEEIRLAFKKNPWCMMISE 645
           + EMGF+P+  +FV A++    ++  T+++KV  Y + GLS  E+   FKK P+ +  SE
Sbjct: 237 IVEMGFDPKSPKFVNALYVFYELSDKTIEEKVNAYIRLGLSVNEVWAVFKKWPFSLKYSE 290

Query: 646 DKI 648
             I
Sbjct: 297 KNI 290

HSP 2 Score: 106.3 bits (264), Expect = 8.2e-23
Identity = 92/390 (23.59%), Postives = 175/390 (44.87%), Query Frame = 1

Query: 406 SLRYLSNSSEIVSSL-KTASLANTAQLENNGKAVIGLLASHGFSESQISDLAKRYPSLFS 465
           ++ YL +S  + + L ++ S+      + N  +V+ LL S+GF +SQIS +   YP    
Sbjct: 57  TVSYLIDSLGLTTKLAESISMKANFDEKGNPDSVLKLLRSYGFKDSQISSIISTYPRFLI 116

Query: 466 ANPDKTILPKLLFFQSKGLSSPEIVKFVCSVPRVLAGSLNKRIIPAFDYIQAVLGSEEKT 525
            NP+KT+  KL F +  G SS E+ + V  VP++L     K I   +DY++ +L  ++ +
Sbjct: 117 ENPEKTLRAKLHFLKLNGASSSELTEIVSKVPKILGKRGGKWISHYYDYVKEILQDQDSS 176

Query: 526 LAAIKRSADILGWDLQI--SVGPNIEILKQTGVP-----------DSNISSYLQ-----Q 585
            ++ KR       ++ +   +G    +L    +            + ++   ++     +
Sbjct: 177 SSSSKRKQTNRNRNVSVLRKLGVPQRLLLNLLISRAKPVCGKERFEESVKKIVEMGFDPK 236

Query: 586 QPKM-------FLTSSIRFKEAVERVTEMGFNPQQMQFVVAV--FCLRAMTKSTLDKKVE 645
            PK        +  S    +E V     +G +  ++  V     F L+   K+ + +K E
Sbjct: 237 SPKFVNALYVFYELSDKTIEEKVNAYIRLGLSVNEVWAVFKKWPFSLKYSEKNII-QKFE 296

Query: 646 VYRKWGLSEEEIRLAFKKNPWCMMISEDKINGAM-------------------------- 705
             ++ GL++EE+ L  KK P C+  SE+KI  ++                          
Sbjct: 297 TLKRVGLTKEEVCLVVKKYPECVGTSEEKIVKSVKTFLELGFTKDEVLMIIKRHPQCIGL 356

Query: 706 ---------DYFVNKIGCQSSYVARRPGLTLYSLKKRLLPRGYIYQVLLSKGLIKKHEYL 733
                    ++ V  +G     VA  P +  +SL+K +LPR  + + L+S GLI +   +
Sbjct: 357 AADSVKKKTEFLVKTMGWPLKVVASTPIVLGFSLEKFVLPRCNVIKALMSNGLIGEMPAI 416

HSP 3 Score: 104.8 bits (260), Expect = 2.4e-22
Identity = 65/228 (28.51%), Postives = 122/228 (53.51%), Query Frame = 1

Query: 44  KSASLASNVVQLKNNRKAIIAFFENHGFSESQVSDLIKKVPLILSANPE-ILFPILLFFK 103
           +S S+ +N  + K N  +++    ++GF +SQ+S +I   P  L  NPE  L   L F K
Sbjct: 73  ESISMKANFDE-KGNPDSVLKLLRSYGFKDSQISSIISTYPRFLIENPEKTLRAKLHFLK 132

Query: 104 SKGLSSPAITKLVCFAPQILKRSLNQEIIPFFDYIQAVLGTVEKTVATIKRFPRILGWNL 163
             G SS  +T++V   P+IL +   + I  ++DY++ +L   + + ++ KR         
Sbjct: 133 LNGASSSELTEIVSKVPKILGKRGGKWISHYYDYVKEILQDQDSSSSSSKR--------K 192

Query: 164 RISVGPNIEILKQFGVPDSNISTYLQRQPKVFSTSSIRFKEIVERVTEMGFNPQRLQFIV 223
           + +   N+ +L++ GVP   +   L  + K       RF+E V+++ EMGF+P+  +F+ 
Sbjct: 193 QTNRNRNVSVLRKLGVPQRLLLNLLISRAKPVCGKE-RFEESVKKIVEMGFDPKSPKFVN 252

Query: 224 AVHALRSLTKSSWDKKLEVYRKWGLSEEEFYLAFRKYPWCMALSEEKI 271
           A++    L+  + ++K+  Y + GLS  E +  F+K+P+ +  SE+ I
Sbjct: 253 ALYVFYELSDKTIEEKVNAYIRLGLSVNEVWAVFKKWPFSLKYSEKNI 290

HSP 4 Score: 96.7 bits (239), Expect = 6.5e-20
Identity = 91/377 (24.14%), Postives = 168/377 (44.56%), Query Frame = 1

Query: 44  KSASLASNVVQLKNNRKAIIAFFENHGFSESQVSDLIKKVPLILSANPE-ILFPILLFFK 103
           +S S+ +N  + K N  +++    ++GF +SQ+S +I   P  L  NPE  L   L F K
Sbjct: 73  ESISMKANFDE-KGNPDSVLKLLRSYGFKDSQISSIISTYPRFLIENPEKTLRAKLHFLK 132

Query: 104 SKGLSSPAITKLVCFAPQILKRSLNQEIIPFFDYIQAVLGTVEKTVATIKR--------- 163
             G SS  +T++V   P+IL +   + I  ++DY++ +L   + + ++ KR         
Sbjct: 133 LNGASSSELTEIVSKVPKILGKRGGKWISHYYDYVKEILQDQDSSSSSSKRKQTNRNRNV 192

Query: 164 -------FPRILGWNLRISVGPNIEILKQFGVPDSNIST--YLQRQPKVFSTSSIRFKEI 223
                   P+ L  NL IS    +   ++F      I    +  + PK F  +   F E+
Sbjct: 193 SVLRKLGVPQRLLLNLLISRAKPVCGKERFEESVKKIVEMGFDPKSPK-FVNALYVFYEL 252

Query: 224 VERVTEMGFNPQRLQFIVAVHALRSLTKSSWD-----------KKLEVYRKWGLSEEEFY 283
            ++  E   N   ++  ++V+ + ++ K  W            +K E  ++ GL++EE  
Sbjct: 253 SDKTIEEKVNAY-IRLGLSVNEVWAVFKK-WPFSLKYSEKNIIQKFETLKRVGLTKEEVC 312

Query: 284 LAFRKYPWCMALSEEKINDTM-----------------------------------DFFI 343
           L  +KYP C+  SEEKI  ++                                   +F +
Sbjct: 313 LVVKKYPECVGTSEEKIVKSVKTFLELGFTKDEVLMIIKRHPQCIGLAADSVKKKTEFLV 372

Query: 344 NKMGRESSLVARRPSLLSYSLKKRLFPRGYVYQVLLSKGLIKKEKNLDLLFEPPEKRFIE 356
             MG    +VA  P +L +SL+K + PR  V + L+S GLI +   +  +   P+ +F++
Sbjct: 373 KTMGWPLKVVASTPIVLGFSLEKFVLPRCNVIKALMSNGLIGEMPAISSVLTSPKLKFLK 432

BLAST of Cucsa.253380 vs. TAIR10
Match: AT1G61970.1 (AT1G61970.1 Mitochondrial transcription termination factor family protein)

HSP 1 Score: 126.7 bits (317), Expect = 5.9e-29
Identity = 88/339 (25.96%), Postives = 174/339 (51.33%), Query Frame = 1

Query: 56  KNNRKAIIAFFENHGFSESQVSDLIKKVPLILSANPE-ILFPILLFFKSKGLSSPAITKL 115
           KNN  +++    +HGF+ SQ+S +I+  P +L A+ E  L P L F +S+G SS  IT++
Sbjct: 81  KNNPDSVLNLLTSHGFTGSQISTIIRDYPQLLIADAEKSLGPKLQFLQSRGASSSEITEI 140

Query: 116 VCFAPQILKRSLNQEIIPFFDYIQAVLGTVEKTVATIKRFPRILGWNLRISVGPNIEILK 175
           V   P+IL +  ++ I  ++D+I+  L  +EK+    K    +   NL   +  N+ +L+
Sbjct: 141 VSSVPEILGKKGHKTISVYYDFIKDTL--LEKSSKNEKLCHSLPQGNLENKIR-NVSVLR 200

Query: 176 QFGVPDSNISTYLQRQPKVFSTSSIRFKEIVERVTEMGFNPQRLQFIVAVHALRSLTKSS 235
           + G+P   + + L    +       +F+E +++V EMGF+P   +F+ A+  +  + + +
Sbjct: 201 ELGMPHKLLFSLLISDSQPVCGKE-KFEETLKKVVEMGFDPTTSKFVEALQVIYKMNEKT 260

Query: 236 WDKKLEVYRKWGLSEEEFYLAFRKYPWCMALSEEKINDTMDFFINK-------------- 295
            ++K+ +Y+  G    + + +F+K+P  + +SE+K+ D+++ F+                
Sbjct: 261 IEEKVHLYKSLGFDVGDVWSSFKKWPISLRVSEKKMLDSIETFLGLGFSRDEFAKMVKHF 320

Query: 296 ---MGRESSLVARRPSLL------------------SYSLKKRLFPRGYVYQVLLSKGLI 355
              +G  +  V ++   L                   YSL+KR+ PRG V + L+SKGL+
Sbjct: 321 PPCIGLSTETVKKKTEFLVKKMNWPLKAVVSNPAVFGYSLEKRIVPRGNVIKALMSKGLM 380

HSP 2 Score: 124.8 bits (312), Expect = 2.2e-28
Identity = 81/253 (32.02%), Postives = 144/253 (56.92%), Query Frame = 1

Query: 406 SLRYLSNSSEIVSSL-KTASLANTAQLENNGKAVIGLLASHGFSESQISDLAKRYPSLFS 465
           ++ YL +S  + + L ++ S   + + +NN  +V+ LL SHGF+ SQIS + + YP L  
Sbjct: 54  TVSYLVDSLGLTTKLAESISRKVSFEDKNNPDSVLNLLTSHGFTGSQISTIIRDYPQLLI 113

Query: 466 ANPDKTILPKLLFFQSKGLSSPEIVKFVCSVPRVLAGSLNKRIIPAFDYIQAVLGSEEKT 525
           A+ +K++ PKL F QS+G SS EI + V SVP +L    +K I   +D+I+  L   EK+
Sbjct: 114 ADAEKSLGPKLQFLQSRGASSSEITEIVSSVPEILGKKGHKTISVYYDFIKDTL--LEKS 173

Query: 526 LAAIKRSADILGWDLQISVGPNIEILKQTGVPDSNISSYL--QQQPKMFLTSSIRFKEAV 585
               K    +   +L+  +  N+ +L++ G+P   + S L    QP   +    +F+E +
Sbjct: 174 SKNEKLCHSLPQGNLENKI-RNVSVLRELGMPHKLLFSLLISDSQP---VCGKEKFEETL 233

Query: 586 ERVTEMGFNPQQMQFVVAVFCLRAMTKSTLDKKVEVYRKWGLSEEEIRLAFKKNPWCMMI 645
           ++V EMGF+P   +FV A+  +  M + T+++KV +Y+  G    ++  +FKK P  + +
Sbjct: 234 KKVVEMGFDPTTSKFVEALQVIYKMNEKTIEEKVHLYKSLGFDVGDVWSSFKKWPISLRV 293

Query: 646 SEDKINGAMDYFV 656
           SE K+  +++ F+
Sbjct: 294 SEKKMLDSIETFL 300

HSP 3 Score: 69.3 bits (168), Expect = 1.1e-11
Identity = 78/351 (22.22%), Postives = 151/351 (43.02%), Query Frame = 1

Query: 391 LHSFFSHGFSESPLKSLRYLSNSSEIVSSLKTASLANTAQLENNGKAVIGLLASHGFSES 450
           L+   SHGF+ S + ++  + +  +++ +    SL    Q           L S G S S
Sbjct: 88  LNLLTSHGFTGSQISTI--IRDYPQLLIADAEKSLGPKLQF----------LQSRGASSS 147

Query: 451 QISDLAKRYPSLFSANPDKTILPKLLFFQSKGLSSPEIVKFVC-SVPRVLAGSLNKRIIP 510
           +I+++    P +      KTI     F +   L      + +C S+P+   G+L  +I  
Sbjct: 148 EITEIVSSVPEILGKKGHKTISVYYDFIKDTLLEKSSKNEKLCHSLPQ---GNLENKIRN 207

Query: 511 AFDYIQAVLGSEEKTLAA--IKRSADILGWDLQISVGPNIEILKQTGVPDSNISSYLQQQ 570
               +   LG   K L +  I  S  + G +        ++ + + G  D   S +++  
Sbjct: 208 VS--VLRELGMPHKLLFSLLISDSQPVCGKE---KFEETLKKVVEMGF-DPTTSKFVEAL 267

Query: 571 PKMFLTSSIRFKEAVERVTEMGFNPQQM--QFVVAVFCLRAMTKSTLDKKVEVYRKWGLS 630
             ++  +    +E V     +GF+   +   F      LR   K  LD  +E +   G S
Sbjct: 268 QVIYKMNEKTIEEKVHLYKSLGFDVGDVWSSFKKWPISLRVSEKKMLDS-IETFLGLGFS 327

Query: 631 EEEIRLAFKKNPWCMMISEDKINGAMDYFVNKIGCQSSYVARRPGLTLYSLKKRLLPRGY 690
            +E     K  P C+ +S + +    ++ V K+      V   P +  YSL+KR++PRG 
Sbjct: 328 RDEFAKMVKHFPPCIGLSTETVKKKTEFLVKKMNWPLKAVVSNPAVFGYSLEKRIVPRGN 387

Query: 691 IYQVLLSKGLIKKH-EYLSSLFNSSENRFIKKFINPH--KEQIPGLLELYK 734
           + + L+SKGL++     +S +   ++  F+ +++  H  K+ +  L+ +Y+
Sbjct: 388 VIKALMSKGLMRNELPSISCVLMCTKQVFLNRYVANHVDKQLVTELMAIYR 416

BLAST of Cucsa.253380 vs. NCBI nr
Match: gi|778688495|ref|XP_011652762.1| (PREDICTED: uncharacterized protein LOC101208771 [Cucumis sativus])

HSP 1 Score: 815.8 bits (2106), Expect = 6.0e-233
Identity = 449/742 (60.51%), Postives = 526/742 (70.89%), Query Frame = 1

Query: 1   MYNLFRRIILPGSPSSVFTHGFSESPLKSLRFLSTSSEIVSSPKSASLASNVVQLKNNRK 60
           M N FRRI+L  SPSSVF+HGFSE PLKSLR+ STSSEIVSSPKSASL S+  +L+NNRK
Sbjct: 1   MSNFFRRILLLRSPSSVFSHGFSEIPLKSLRYFSTSSEIVSSPKSASLPSHAAKLENNRK 60

Query: 61  AIIAFFENHGFSESQVSDLIKKVPLILSANPE-ILFPILLFFKSKGLSSPAITKLVCFAP 120
           A+IA   NHGFS+SQ+SD++K+ P ILSANPE  L P L FF+SKG SSP I K +   P
Sbjct: 61  AVIALLANHGFSQSQISDVVKRYPGILSANPEKTLLPKLSFFQSKGFSSPEIVKFLRSNP 120

Query: 121 QILKRSLNQEIIPFFDYIQAVLGTVEKTVATIKRFPRILGWNLRISVGPNIEILKQFGVP 180
             L+ SLN+ IIP FDYIQAV G+ EKT+A IK+F  IL  +LRISVGPNIEILKQ GVP
Sbjct: 121 WSLRASLNKRIIPAFDYIQAVFGSEEKTLAVIKQFVGILVKDLRISVGPNIEILKQIGVP 180

Query: 181 DSNISTYLQRQPKVFSTSSIRFKEIVERVTEMGFNPQRLQFIVAVHALRSLTKSSWDKKL 240
           DSNI  YLQ QP+VF  + I FKE VERV EMGFNPQ+LQF+VAV ALRS+TKS+WDKK+
Sbjct: 181 DSNILRYLQYQPRVFLKNPILFKETVERVVEMGFNPQQLQFVVAVFALRSMTKSTWDKKV 240

Query: 241 EVYRKWGLSEEEFYLAFRKYPWCMALSEEKINDTMDFFINKMGRESSLVARRPSLLSYSL 300
            VYRKWG SEEE  L+FRK+PWCM  SE+KIN  MDFF+NKMG  SS+ ARRP LLS SL
Sbjct: 241 AVYRKWGFSEEEIRLSFRKHPWCMMRSEDKINGVMDFFVNKMGFNSSVAARRPVLLSLSL 300

Query: 301 KKRLFPRGYVYQVLLSKGLIKKEKNLDLLFEPPEKRFIEKYINAHKEQVPGLLELYEQKL 360
           KKR+FPRGYVYQVL+SKGLIKK  N  L FE PE  FIEK+IN HKEQ+PGLLE YEQKL
Sbjct: 301 KKRIFPRGYVYQVLVSKGLIKKHHNPLLFFESPENCFIEKFINPHKEQIPGLLESYEQKL 360

Query: 361 MNLEDEQAFVVSPFLLLNKEPTGLSSFSSHFLHSFFSHGFSESPLKSLRYLSNSSEIVSS 420
           M+                         S  F HS    G + S    L  LS  S ++  
Sbjct: 361 MD-------------------------SRSFSHSSSQQGTNRSGFLLLPVLSVMSNLLRR 420

Query: 421 LKTASLANTAQLENNGKAVIGLLASHGFSESQISDLAKRYPSLFSANPDKTILPKLLFFQ 480
           +      ++             + SHGFSES +  L     + FS +             
Sbjct: 421 ILLLRSPSS-------------VFSHGFSESPLKSL-----TYFSVS------------- 480

Query: 481 SKGLSSPEIVKFVCSVPRVLAGSLNKRIIPAFDYIQAVLGSEEKTLAAIKRSADILGWDL 540
           S+ +SSP+      +  R+     N++ + A           EKTLA IKRSADIL WD 
Sbjct: 481 SEIVSSPKSASLASNAVRL---ENNRKDVIAL-LANHGFSDYEKTLAVIKRSADILFWDF 540

Query: 541 QISVGPNIEILKQTGVPDSNISSYLQQQPKMFLTSSIRFKEAVERVTEMGFNPQQMQFVV 600
           QISVGPNIEILKQ GVPDSNI  YL  QP++FLT+SIRFKE VERVTEMGFNP+ + FV+
Sbjct: 541 QISVGPNIEILKQIGVPDSNILKYLHYQPRVFLTNSIRFKETVERVTEMGFNPRGLLFVI 600

Query: 601 AVFCLRAMTKSTLDKKVEVYRKWGLSEEEIRLAFKKNPWCMMISEDKINGAMDYFVNKIG 660
           AVF LR+MTKST DKKVEVYR WGLSEEEI LAF++NPWCM+ SEDKINGAMD++VNK+G
Sbjct: 601 AVFALRSMTKSTWDKKVEVYRNWGLSEEEIHLAFRRNPWCMIASEDKINGAMDFYVNKMG 660

Query: 661 CQSSYVARRPGLTLYSLKKRLLPRGYIYQVLLSKGLIKKHEYLSSLFNSSENRFIKKFIN 720
           C SS+ ARRP L   SLKKR+LPRGY+YQVLLSKGLIKK E +  LF S E RFI+K+IN
Sbjct: 661 CDSSFAARRPVLLQLSLKKRILPRGYVYQVLLSKGLIKKTENICLLFESPEKRFIEKYIN 682

Query: 721 PHKEQIPGLLELYKERLMDSRR 742
           PHKEQIPGLLELY+++LMDSRR
Sbjct: 721 PHKEQIPGLLELYEQKLMDSRR 682

BLAST of Cucsa.253380 vs. NCBI nr
Match: gi|778678818|ref|XP_011651040.1| (PREDICTED: uncharacterized protein LOC101221269 isoform X2 [Cucumis sativus])

HSP 1 Score: 717.6 bits (1851), Expect = 2.2e-203
Identity = 364/367 (99.18%), Postives = 367/367 (100.00%), Query Frame = 1

Query: 1   MYNLFRRIILPGSPSSVFTHGFSESPLKSLRFLSTSSEIVSSPKSASLASNVVQLKNNRK 60
           MYNLFRRIILPGSPSSVFTHGFSESPLKSLRFLSTSSEIVSSPKSASLASNVVQLKNNRK
Sbjct: 1   MYNLFRRIILPGSPSSVFTHGFSESPLKSLRFLSTSSEIVSSPKSASLASNVVQLKNNRK 60

Query: 61  AIIAFFENHGFSESQVSDLIKKVPLILSANPEILFPILLFFKSKGLSSPAITKLVCFAPQ 120
           AIIAFFENHGFSESQ+SDLIKKVPLILSANPEILFPILLFFKSKGLSSPAITKLVCFAPQ
Sbjct: 61  AIIAFFENHGFSESQISDLIKKVPLILSANPEILFPILLFFKSKGLSSPAITKLVCFAPQ 120

Query: 121 ILKRSLNQEIIPFFDYIQAVLGTVEKTVATIKRFPRILGWNLRISVGPNIEILKQFGVPD 180
           ILKRSLNQEIIPFFDYIQAVLGTVEKTVATIKRFPRILGWNLRISVGPNIEILKQFGVPD
Sbjct: 121 ILKRSLNQEIIPFFDYIQAVLGTVEKTVATIKRFPRILGWNLRISVGPNIEILKQFGVPD 180

Query: 181 SNISTYLQRQPKVFSTSSIRFKEIVERVTEMGFNPQRLQFIVAVHALRSLTKSSWDKKLE 240
           SNISTYLQRQPKVFSTSSIRFKEIVERVTEMGFNPQRLQFIVAVHALRSLTKSSWDKK+E
Sbjct: 181 SNISTYLQRQPKVFSTSSIRFKEIVERVTEMGFNPQRLQFIVAVHALRSLTKSSWDKKIE 240

Query: 241 VYRKWGLSEEEFYLAFRKYPWCMALSEEKINDTMDFFINKMGRESSLVARRPSLLSYSLK 300
           VYRKWGLSEEEFYLAFRKYPWCMALSE+KINDTMDFFINKMGRESSLVARRPSLLSYSLK
Sbjct: 241 VYRKWGLSEEEFYLAFRKYPWCMALSEDKINDTMDFFINKMGRESSLVARRPSLLSYSLK 300

Query: 301 KRLFPRGYVYQVLLSKGLIKKEKNLDLLFEPPEKRFIEKYINAHKEQVPGLLELYEQKLM 360
           KRLFPRGYVYQVLLSKGLIKKEKNLDLLFEPPEKRFIEKYINAHKEQVPGLLELYEQKLM
Sbjct: 301 KRLFPRGYVYQVLLSKGLIKKEKNLDLLFEPPEKRFIEKYINAHKEQVPGLLELYEQKLM 360

Query: 361 NLEDEQA 368
           NLEDEQA
Sbjct: 361 NLEDEQA 367

BLAST of Cucsa.253380 vs. NCBI nr
Match: gi|778678818|ref|XP_011651040.1| (PREDICTED: uncharacterized protein LOC101221269 isoform X2 [Cucumis sativus])

HSP 1 Score: 480.3 bits (1235), Expect = 6.0e-132
Identity = 244/347 (70.32%), Postives = 287/347 (82.71%), Query Frame = 1

Query: 393 SFFSHGFSESPLKSLRYLSNSSEIVSSLKTASLA-NTAQLENNGKAVIGLLASHGFSESQ 452
           S F+HGFSESPLKSLR+LS SSEIVSS K+ASLA N  QL+NN KA+I    +HGFSESQ
Sbjct: 16  SVFTHGFSESPLKSLRFLSTSSEIVSSPKSASLASNVVQLKNNRKAIIAFFENHGFSESQ 75

Query: 453 ISDLAKRYPSLFSANPDKTILPKLLFFQSKGLSSPEIVKFVCSVPRVLAGSLNKRIIPAF 512
           ISDL K+ P + SANP+  + P LLFF+SKGLSSP I K VC  P++L  SLN+ IIP F
Sbjct: 76  ISDLIKKVPLILSANPE-ILFPILLFFKSKGLSSPAITKLVCFAPQILKRSLNQEIIPFF 135

Query: 513 DYIQAVLGSEEKTLAAIKRSADILGWDLQISVGPNIEILKQTGVPDSNISSYLQQQPKMF 572
           DYIQAVLG+ EKT+A IKR   ILGW+L+ISVGPNIEILKQ GVPDSNIS+YLQ+QPK+F
Sbjct: 136 DYIQAVLGTVEKTVATIKRFPRILGWNLRISVGPNIEILKQFGVPDSNISTYLQRQPKVF 195

Query: 573 LTSSIRFKEAVERVTEMGFNPQQMQFVVAVFCLRAMTKSTLDKKVEVYRKWGLSEEEIRL 632
            TSSIRFKE VERVTEMGFNPQ++QF+VAV  LR++TKS+ DKK+EVYRKWGLSEEE  L
Sbjct: 196 STSSIRFKEIVERVTEMGFNPQRLQFIVAVHALRSLTKSSWDKKIEVYRKWGLSEEEFYL 255

Query: 633 AFKKNPWCMMISEDKINGAMDYFVNKIGCQSSYVARRPGLTLYSLKKRLLPRGYIYQVLL 692
           AF+K PWCM +SEDKIN  MD+F+NK+G +SS VARRP L  YSLKKRL PRGY+YQVLL
Sbjct: 256 AFRKYPWCMALSEDKINDTMDFFINKMGRESSLVARRPSLLSYSLKKRLFPRGYVYQVLL 315

Query: 693 SKGLIKKHEYLSSLFNSSENRFIKKFINPHKEQIPGLLELYKERLMD 739
           SKGLIKK + L  LF   E RFI+K+IN HKEQ+PGLLELY+++LM+
Sbjct: 316 SKGLIKKEKNLDLLFEPPEKRFIEKYINAHKEQVPGLLELYEQKLMN 361

HSP 2 Score: 716.8 bits (1849), Expect = 3.8e-203
Identity = 363/369 (98.37%), Postives = 367/369 (99.46%), Query Frame = 1

Query: 1   MYNLFRRIILPGSPSSVFTHGFSESPLKSLRFLSTSSEIVSSPKSASLASNVVQLKNNRK 60
           MYNLFRRIILPGSPSSVFTHGFSESPLKSLRFLSTSSEIVSSPKSASLASNVVQLKNNRK
Sbjct: 1   MYNLFRRIILPGSPSSVFTHGFSESPLKSLRFLSTSSEIVSSPKSASLASNVVQLKNNRK 60

Query: 61  AIIAFFENHGFSESQVSDLIKKVPLILSANPEILFPILLFFKSKGLSSPAITKLVCFAPQ 120
           AIIAFFENHGFSESQ+SDLIKKVPLILSANPEILFPILLFFKSKGLSSPAITKLVCFAPQ
Sbjct: 61  AIIAFFENHGFSESQISDLIKKVPLILSANPEILFPILLFFKSKGLSSPAITKLVCFAPQ 120

Query: 121 ILKRSLNQEIIPFFDYIQAVLGTVEKTVATIKRFPRILGWNLRISVGPNIEILKQFGVPD 180
           ILKRSLNQEIIPFFDYIQAVLGTVEKTVATIKRFPRILGWNLRISVGPNIEILKQFGVPD
Sbjct: 121 ILKRSLNQEIIPFFDYIQAVLGTVEKTVATIKRFPRILGWNLRISVGPNIEILKQFGVPD 180

Query: 181 SNISTYLQRQPKVFSTSSIRFKEIVERVTEMGFNPQRLQFIVAVHALRSLTKSSWDKKLE 240
           SNISTYLQRQPKVFSTSSIRFKEIVERVTEMGFNPQRLQFIVAVHALRSLTKSSWDKK+E
Sbjct: 181 SNISTYLQRQPKVFSTSSIRFKEIVERVTEMGFNPQRLQFIVAVHALRSLTKSSWDKKIE 240

Query: 241 VYRKWGLSEEEFYLAFRKYPWCMALSEEKINDTMDFFINKMGRESSLVARRPSLLSYSLK 300
           VYRKWGLSEEEFYLAFRKYPWCMALSE+KINDTMDFFINKMGRESSLVARRPSLLSYSLK
Sbjct: 241 VYRKWGLSEEEFYLAFRKYPWCMALSEDKINDTMDFFINKMGRESSLVARRPSLLSYSLK 300

Query: 301 KRLFPRGYVYQVLLSKGLIKKEKNLDLLFEPPEKRFIEKYINAHKEQVPGLLELYEQKLM 360
           KRLFPRGYVYQVLLSKGLIKKEKNLDLLFEPPEKRFIEKYINAHKEQVPGLLELYEQKLM
Sbjct: 301 KRLFPRGYVYQVLLSKGLIKKEKNLDLLFEPPEKRFIEKYINAHKEQVPGLLELYEQKLM 360

Query: 361 NLEDEQAFV 370
           NLEDEQ  +
Sbjct: 361 NLEDEQILI 369

BLAST of Cucsa.253380 vs. NCBI nr
Match: gi|778678813|ref|XP_011651039.1| (PREDICTED: uncharacterized protein LOC101221269 isoform X1 [Cucumis sativus])

HSP 1 Score: 480.3 bits (1235), Expect = 6.0e-132
Identity = 244/347 (70.32%), Postives = 287/347 (82.71%), Query Frame = 1

Query: 393 SFFSHGFSESPLKSLRYLSNSSEIVSSLKTASLA-NTAQLENNGKAVIGLLASHGFSESQ 452
           S F+HGFSESPLKSLR+LS SSEIVSS K+ASLA N  QL+NN KA+I    +HGFSESQ
Sbjct: 16  SVFTHGFSESPLKSLRFLSTSSEIVSSPKSASLASNVVQLKNNRKAIIAFFENHGFSESQ 75

Query: 453 ISDLAKRYPSLFSANPDKTILPKLLFFQSKGLSSPEIVKFVCSVPRVLAGSLNKRIIPAF 512
           ISDL K+ P + SANP+  + P LLFF+SKGLSSP I K VC  P++L  SLN+ IIP F
Sbjct: 76  ISDLIKKVPLILSANPE-ILFPILLFFKSKGLSSPAITKLVCFAPQILKRSLNQEIIPFF 135

Query: 513 DYIQAVLGSEEKTLAAIKRSADILGWDLQISVGPNIEILKQTGVPDSNISSYLQQQPKMF 572
           DYIQAVLG+ EKT+A IKR   ILGW+L+ISVGPNIEILKQ GVPDSNIS+YLQ+QPK+F
Sbjct: 136 DYIQAVLGTVEKTVATIKRFPRILGWNLRISVGPNIEILKQFGVPDSNISTYLQRQPKVF 195

Query: 573 LTSSIRFKEAVERVTEMGFNPQQMQFVVAVFCLRAMTKSTLDKKVEVYRKWGLSEEEIRL 632
            TSSIRFKE VERVTEMGFNPQ++QF+VAV  LR++TKS+ DKK+EVYRKWGLSEEE  L
Sbjct: 196 STSSIRFKEIVERVTEMGFNPQRLQFIVAVHALRSLTKSSWDKKIEVYRKWGLSEEEFYL 255

Query: 633 AFKKNPWCMMISEDKINGAMDYFVNKIGCQSSYVARRPGLTLYSLKKRLLPRGYIYQVLL 692
           AF+K PWCM +SEDKIN  MD+F+NK+G +SS VARRP L  YSLKKRL PRGY+YQVLL
Sbjct: 256 AFRKYPWCMALSEDKINDTMDFFINKMGRESSLVARRPSLLSYSLKKRLFPRGYVYQVLL 315

Query: 693 SKGLIKKHEYLSSLFNSSENRFIKKFINPHKEQIPGLLELYKERLMD 739
           SKGLIKK + L  LF   E RFI+K+IN HKEQ+PGLLELY+++LM+
Sbjct: 316 SKGLIKKEKNLDLLFEPPEKRFIEKYINAHKEQVPGLLELYEQKLMN 361

HSP 2 Score: 603.2 bits (1554), Expect = 6.1e-169
Identity = 305/361 (84.49%), Postives = 332/361 (91.97%), Query Frame = 1

Query: 1   MYNLFRRIILPGSPSSVFTHGFSESPLKSLRFLSTSSEIVSSPKSASLASNVVQLKNNRK 60
           MYNLFRRIILPGSPSSVFTHGFSESPLKSLRFLSTSSEIVSSPKSASLASNVVQLKNNRK
Sbjct: 1   MYNLFRRIILPGSPSSVFTHGFSESPLKSLRFLSTSSEIVSSPKSASLASNVVQLKNNRK 60

Query: 61  AIIAFFENHGFSESQVSDLIKKVPLILSANPEILFPILLFFKSKGLSSPAITKLVCFAPQ 120
           AIIAFFENHGFSESQ+SDLIKKVPLILSANPEILFPILLFFKSKGLSSPAITKLVCFAPQ
Sbjct: 61  AIIAFFENHGFSESQISDLIKKVPLILSANPEILFPILLFFKSKGLSSPAITKLVCFAPQ 120

Query: 121 ILKRSLNQEIIPFFDYIQAVLGTVEKTVATIKRFPRILGWNLRISVGPNIEILKQFGVPD 180
           ILKRSLNQEIIPFFDYIQAVLGTVEKTVATIKRFPRILGWNL  SVGPNIEILKQ GVPD
Sbjct: 121 ILKRSLNQEIIPFFDYIQAVLGTVEKTVATIKRFPRILGWNL--SVGPNIEILKQIGVPD 180

Query: 181 SNISTYLQRQPKVFSTSSIRFKEIVERVTEMGFNPQRLQFIVAVHALRSLTKSSWDKKLE 240
           SNIS+YLQ+QPK+F TSSIRFKE VERVTEMGFNPQ++QF+VAV  LR++TKS+ DKK+E
Sbjct: 181 SNISSYLQQQPKMFLTSSIRFKEAVERVTEMGFNPQQMQFVVAVFCLRAMTKSTLDKKVE 240

Query: 241 VYRKWGLSEEEFYLAFRKYPWCMALSEEKINDTMDFFINKMGRESSLVARRPSLLSYSLK 300
           VYRKWGLSEEE  LAF+K PWCM +SE+KIN  MD+F+NK+G +SS VARRP L  YSLK
Sbjct: 241 VYRKWGLSEEEIRLAFKKNPWCMMISEDKINGAMDYFVNKIGCQSSYVARRPGLTLYSLK 300

Query: 301 KRLFPRGYVYQVLLSKGLIKKEKNLDLLFEPPEKRFIEKYINAHKEQVPGLLELYEQKLM 360
           KRL PRGY+YQVLLSKGLIKK + L  LF   E RFI+K+IN HKEQ+PGLLELY+++LM
Sbjct: 301 KRLLPRGYIYQVLLSKGLIKKHEYLSSLFNSSENRFIKKFINPHKEQIPGLLELYKERLM 359

Query: 361 N 362
           +
Sbjct: 361 D 359

BLAST of Cucsa.253380 vs. NCBI nr
Match: gi|778678821|ref|XP_011651041.1| (PREDICTED: uncharacterized protein LOC101221269 isoform X3 [Cucumis sativus])

HSP 1 Score: 572.4 bits (1474), Expect = 1.2e-159
Identity = 297/350 (84.86%), Postives = 314/350 (89.71%), Query Frame = 1

Query: 393 SFFSHGFSESPLKSLRYLSNSSEIVSSLKTASLA-NTAQLENNGKAVIGLLASHGFSESQ 452
           S F+HGFSESPLKSLR+LS SSEIVSS K+ASLA N  QL+NN KA+I    +HGFSESQ
Sbjct: 16  SVFTHGFSESPLKSLRFLSTSSEIVSSPKSASLASNVVQLKNNRKAIIAFFENHGFSESQ 75

Query: 453 ISDLAKRYPSLFSANPDKTILPKLLFFQSKGLSSPEIVKFVCSVPRVLAGSLNKRIIPAF 512
           ISDL K+ P + SANP+  + P LLFF+SKGLSSP I K VC  P++L  SLN+ IIP F
Sbjct: 76  ISDLIKKVPLILSANPE-ILFPILLFFKSKGLSSPAITKLVCFAPQILKRSLNQEIIPFF 135

Query: 513 DYIQAVLGSEEKTLAAIKRSADILGWDLQISVGPNIEILKQTGVPDSNISSYLQQQPKMF 572
           DYIQAVLG+ EKT+A IKR   ILGW+L  SVGPNIEILKQ GVPDSNISSYLQQQPKMF
Sbjct: 136 DYIQAVLGTVEKTVATIKRFPRILGWNL--SVGPNIEILKQIGVPDSNISSYLQQQPKMF 195

Query: 573 LTSSIRFKEAVERVTEMGFNPQQMQFVVAVFCLRAMTKSTLDKKVEVYRKWGLSEEEIRL 632
           LTSSIRFKEAVERVTEMGFNPQQMQFVVAVFCLRAMTKSTLDKKVEVYRKWGLSEEEIRL
Sbjct: 196 LTSSIRFKEAVERVTEMGFNPQQMQFVVAVFCLRAMTKSTLDKKVEVYRKWGLSEEEIRL 255

Query: 633 AFKKNPWCMMISEDKINGAMDYFVNKIGCQSSYVARRPGLTLYSLKKRLLPRGYIYQVLL 692
           AFKKNPWCMMISEDKINGAMDYFVNKIGCQSSYVARRPGLTLYSLKKRLLPRGYIYQVLL
Sbjct: 256 AFKKNPWCMMISEDKINGAMDYFVNKIGCQSSYVARRPGLTLYSLKKRLLPRGYIYQVLL 315

Query: 693 SKGLIKKHEYLSSLFNSSENRFIKKFINPHKEQIPGLLELYKERLMDSRR 742
           SKGLIKKHEYLSSLFNSSENRFIKKFINPHKEQIPGLLELYKERLMDSRR
Sbjct: 316 SKGLIKKHEYLSSLFNSSENRFIKKFINPHKEQIPGLLELYKERLMDSRR 362

HSP 2 Score: 599.7 bits (1545), Expect = 6.7e-168
Identity = 305/361 (84.49%), Postives = 329/361 (91.14%), Query Frame = 1

Query: 1   MYNLFRRIILPGSPSSVFTHGFSESPLKSLRFLSTSSEIVSSPKSASLASNVVQLKNNRK 60
           MYNLFRRIIL  SPSSV THGFSESPLKSLRFLSTSSEIVSSPKSASLASNVVQLKNNRK
Sbjct: 1   MYNLFRRIILLRSPSSVCTHGFSESPLKSLRFLSTSSEIVSSPKSASLASNVVQLKNNRK 60

Query: 61  AIIAFFENHGFSESQVSDLIKKVPLILSANPEILFPILLFFKSKGLSSPAITKLVCFAPQ 120
            +IAFFENHGFSESQ+SDL+KKVPLILSAN E LFP+LLFFKSKGLSSP ITK VC APQ
Sbjct: 61  TVIAFFENHGFSESQISDLVKKVPLILSANTETLFPVLLFFKSKGLSSPEITKFVCSAPQ 120

Query: 121 ILKRSLNQEIIPFFDYIQAVLGTVEKTVATIKRFPRILGWNLRISVGPNIEILKQFGVPD 180
           +LKRSLNQEIIP FDYIQAVLGTVEKTVATIKRF  ILGWNLRISVGPNIEIL+QFGVPD
Sbjct: 121 VLKRSLNQEIIPVFDYIQAVLGTVEKTVATIKRFAGILGWNLRISVGPNIEILRQFGVPD 180

Query: 181 SNISTYLQRQPKVFSTSSIRFKEIVERVTEMGFNPQRLQFIVAVHALRSLTKSSWDKKLE 240
           SNIS YLQRQPK+FS SSI+FKE+VERV EMGFNPQ+LQF+VAV ALRS+TKSSWDKK+E
Sbjct: 181 SNISFYLQRQPKMFSISSIQFKEVVERVMEMGFNPQQLQFVVAVFALRSMTKSSWDKKVE 240

Query: 241 VYRKWGLSEEEFYLAFRKYPWCMALSEEKINDTMDFFINKMGRESSLVARRPSLLSYSLK 300
           VYRKWGLSEEEF LAFR++P CM  SE+KIN  MDFF+N++G ESS VARRP L+S SLK
Sbjct: 241 VYRKWGLSEEEFSLAFRRHPLCMTFSEDKINGVMDFFVNEIGWESSFVARRPVLISLSLK 300

Query: 301 KRLFPRGYVYQVLLSKGLIKKEKNLDLLFEPPEKRFIEKYINAHKEQVPGLLELYEQKLM 360
           KRLFPRGYVYQVLLSKGLIKK K L LLFE PE RFIEK+IN HKEQ+PGLLELY+QKLM
Sbjct: 301 KRLFPRGYVYQVLLSKGLIKKHKKLYLLFEYPEIRFIEKFINPHKEQIPGLLELYKQKLM 360

Query: 361 N 362
           +
Sbjct: 361 D 361