Cucsa.253260 (gene) Cucumber (Gy14) v1

Overview
NameCucsa.253260
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionProtein TIC 214
Locationscaffold02229 : 1925118 .. 1925580 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ACTAAAAGAATATTTACCAAAAATATATGATCCGTTTTTGAGTGGTCCTTACCGAAGACGAATCAATTTACTTTTAGGATTCTCAATGCTAAATGAAACTTCTAGAAAAAATGATAGTGAGAACATTTGGACAAATAAGATTCATGCTATCATTCGTAATAATAACAACGGTTTCTTCGATGGAGTTACAATTCCTTACAAGGCTAACGCAAGTGATCGAAACTCGATTGACCTAACAGAAATCAGTAAAAAAGTTCCTCGCTGGTCATACAACTTAATCAAGGAGATAGAACAAGTGTACGACATAACCGATGGAGAGCTCGTGTTAGATTATCAAATTCGTTCAAGAAAATGCAAACGTGTAGTAATTTTAACTGACCTTAAGAAAATTAAGAAGATTTCTACTTCTACTTCTACTCCTACTTCTACTCCTGCTTCTACTCCTGCTTCTTTTTTCATTTAG

mRNA sequence

ACTAAAAGAATATTTACCAAAAATATATGATCCGTTTTTGAGTGGTCCTTACCGAAGACGAATCAATTTACTTTTAGGATTCTCAATGCTAAATGAAACTTCTAGAAAAAATGATATTACAATTCCTTACAAGGCTAACGCAAGTGATCGAAACTCGATTGACCTAACAGAAATCAGTAAAAAAGTTCCTCGCTGGTCATACAACTTAATCAAGGAGATAGAACAAGTGTACGACATAACCGATGGAGAGCTCGTGTTAGATTATCAAATTCGTTCAAGAAAATGCAAACGTGTAGTAATTTTAACTGACCTTAAGAAAATTAAGAAGATTTCTACTTCTACTTCTACTCCTACTTCTACTCCTGCTTCTACTCCTGCTTCTTTTTTCATTTAG

Coding sequence (CDS)

ACTAAAAGAATATTTACCAAAAATATATGATCCGTTTTTGAGTGGTCCTTACCGAAGACGAATCAATTTACTTTTAGGATTCTCAATGCTAAATGAAACTTCTAGAAAAAATGATATTACAATTCCTTACAAGGCTAACGCAAGTGATCGAAACTCGATTGACCTAACAGAAATCAGTAAAAAAGTTCCTCGCTGGTCATACAACTTAATCAAGGAGATAGAACAAGTGTACGACATAACCGATGGAGAGCTCGTGTTAGATTATCAAATTCGTTCAAGAAAATGCAAACGTGTAGTAATTTTAACTGACCTTAAGAAAATTAAGAAGATTTCTACTTCTACTTCTACTCCTACTTCTACTCCTGCTTCTACTCCTGCTTCTTTTTTCATTTAG

Protein sequence

LKEYLPKIYDPFLSGPYRRRINLLLGFSMLNETSRKNDITIPYKANASDRNSIDLTEISKKVPRWSYNLIKEIEQVYDITDGELVLDYQIRSRKCKRVVILTDLKKIKKISTSTSTPTSTPASTPASFFI*
Homology
[Close]
BLAST of Cucsa.253260 vs. Swiss-Prot
Match: TI214_CUCSA (Protein TIC 214 OS=Cucumis sativus GN=TIC214 PE=3 SV=2)

HSP 1 Score: 219.2 bits (557), Expect = 2.7e-56
Identity = 117/149 (78.52%), Postives = 120/149 (80.54%), Query Frame = 1 

Query: 2   KEYLPKIYDPFLSGPYRRRINLLLGFSMLNETSRKND----------------------- 61
           KEYLPKIYDPFLSGPYRRRINLLLGFSMLNETSRKND                       
Sbjct: 455 KEYLPKIYDPFLSGPYRRRINLLLGFSMLNETSRKNDSENIWTNKIHAIIRNNNNGFFDG 514

Query: 62  ITIPYKANASDRNSIDLTEISKKVPRWSYNLIKEIEQVYDITDGELVLDYQIRSRKCKRV 121
           +TIPYKANASD+NSIDLTEISKKVPRWSY LIKEIEQVYDITDGELVLDYQIRSRKCKRV
Sbjct: 515 VTIPYKANASDQNSIDLTEISKKVPRWSYKLIKEIEQVYDITDGELVLDYQIRSRKCKRV 574

Query: 122 VILTDLKKIKKISTSTSTPTSTPASTPAS 128
           VIL+DL KIKKI  STSTPTSTP STP S
Sbjct: 575 VILSDLNKIKKI--STSTPTSTPTSTPTS 601


HSP 2 Score: 217.2 bits (552), Expect = 1.0e-55
Identity = 119/165 (72.12%), Postives = 122/165 (73.94%), Query Frame = 1 

Query: 2   KEYLPKIYDPFLSGPYRRRINLLLGFSMLNETSRKND----------------------- 61
           KEYLPKIYDPFLSGPYRRRINLLLGFSMLNETSRKND                       
Sbjct: 455 KEYLPKIYDPFLSGPYRRRINLLLGFSMLNETSRKNDSENIWTNKIHAIIRNNNNGFFDG 514

Query: 62  ITIPYKANASDRNSIDLTEISKKVPRWSYNLIKEIEQVYDITDGELVLDYQIRSRKCKRV 121
           +TIPYKANASD+NSIDLTEISKKVPRWSY LIKEIEQVYDITDGELVLDYQIRSRKCKRV
Sbjct: 515 VTIPYKANASDQNSIDLTEISKKVPRWSYKLIKEIEQVYDITDGELVLDYQIRSRKCKRV 574

Query: 122 VILTDLKKIKKISTST----------------STPTSTPASTPAS 128
           VIL+DL KIKKISTST                STPTSTP STP S
Sbjct: 575 VILSDLNKIKKISTSTPTSTPTSTPTSTSTPTSTPTSTPTSTPTS 619


HSP 3 Score: 27.7 bits (60), Expect = 1.2e+02
Identity = 13/17 (76.47%), Postives = 13/17 (76.47%), Query Frame = 1 

Query: 111 STSTSTPTSTPASTPAS 128
           STSTSTP S PAST  S
Sbjct: 619 STSTSTPASIPASTSTS 635

[Close]
BLAST of Cucsa.253260 vs. Swiss-Prot
Match: TI214_POPTR (Protein TIC 214 OS=Populus trichocarpa GN=TIC214 PE=3 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 1.9e-12
Identity = 52/156 (33.33%), Postives = 73/156 (46.79%), Query Frame = 1 

Query: 2   KEYLPKIYDPFLSGPYRRRINLLLGFSMLN--------------------------ETSR 61
           KEYLPKIYDPFLSG YR ++N     S++N                          E   
Sbjct: 494 KEYLPKIYDPFLSGLYRGKVNFFFSPSIINEISIKNYIEMLWINKIYLILLISNYQEFEP 553

Query: 62  KND------------ITIPYKANASDRNSIDLT-EISKKVPRWSYNLIKEIEQVYDITDG 119
           K +            I +  K     + S ++  EISK++PRWSY LI ++EQ     + 
Sbjct: 554 KMEDRIRILKFLFDAIIVDSKNKKIVKKSTEIKKEISKQIPRWSYKLIDDLEQQEGENEE 613

[Close]
BLAST of Cucsa.253260 vs. Swiss-Prot
Match: TI214_POPAL (Protein TIC 214 OS=Populus alba GN=TIC214 PE=3 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 1.9e-12
Identity = 52/156 (33.33%), Postives = 73/156 (46.79%), Query Frame = 1 

Query: 2   KEYLPKIYDPFLSGPYRRRINLLLGFSMLN--------------------------ETSR 61
           KEYLPKIYDPFLSG YR ++N     S++N                          E   
Sbjct: 494 KEYLPKIYDPFLSGLYRGKVNFFFSPSIINEISIKNYIEMLWINKIYLILLISNYQEFEP 553

Query: 62  KND------------ITIPYKANASDRNSIDLT-EISKKVPRWSYNLIKEIEQVYDITDG 119
           K +            I +  K     + S ++  EISK++PRWSY LI ++EQ     + 
Sbjct: 554 KMEDRIRILKFLFDAIIVDSKNKKIVKKSTEIKKEISKQIPRWSYKLIDDLEQQEGENEE 613

[Close]
BLAST of Cucsa.253260 vs. Swiss-Prot
Match: TI214_RANMC (Protein TIC 214 OS=Ranunculus macranthus GN=TIC214 PE=3 SV=2)

HSP 1 Score: 63.9 bits (154), Expect = 1.5e-09
Identity = 46/122 (37.70%), Postives = 61/122 (50.00%), Query Frame = 1 

Query: 2   KEYLPKIYDPFLSGPYRRRINLLLGFSMLNE--------------TSRKNDI-TIPYKAN 61
           +E LPKIYDP L+GPYR  I  L   S +NE               ++  DI    +K  
Sbjct: 492 QECLPKIYDPLLNGPYRGTIKELYSRSSMNEYLIPSIEDPRDTIWINKLYDIFPTDFKEL 551

Query: 62  ASDRNSIDLTEISKKVPRWSYNLIKEIEQVYDITDGELVLDYQIRSRKCKRVVILTDLKK 109
              +    + EI KKV +WSY LI ++E+  +  + E   D  IRSRK KRVVI  D + 
Sbjct: 552 KKKKIGKRINEIKKKVFQWSYKLIDDLEE--EEEEEETTEDRGIRSRKAKRVVIFNDNET 611

[Close]
BLAST of Cucsa.253260 vs. Swiss-Prot
Match: TI214_NANDO (Protein TIC 214 OS=Nandina domestica GN=TIC214 PE=3 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 1.9e-09
Identity = 39/92 (42.39%), Postives = 51/92 (55.43%), Query Frame = 1 

Query: 33  TSRKNDITIPYKANASDRNSIDLTEISKKVPRWSYNLIKEIEQVYDITDGELVLDYQIRS 92
           T+  ND TI          S+++ EI K VPRW Y L  ++E+     D E   D+ IRS
Sbjct: 519 TTYPNDQTI-----IDIEESLEINEIKKTVPRWLYKLTDDLEEEEIENDEESKPDHGIRS 578

Query: 93  RKCKRVVILTDLKKIKKISTSTSTPTSTPAST 125
           RK KRVVI TD +K +   TST+  TST  +T
Sbjct: 579 RKAKRVVIFTDNEKNQNTPTSTTETTSTAETT 605

[Close]
BLAST of Cucsa.253260 vs. TrEMBL
Match: G3ETV7_CUCME (Hypothetical chloroplast RF19 OS=Cucumis melo subsp. melo GN=ycf1 PE=4 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 3.5e-18
Identity = 62/163 (38.04%), Postives = 79/163 (48.47%), Query Frame = 1 

Query: 2   KEYLPKIYDPFLSGPYRRRINLLLGFSMLN------------------------------ 61
           K+YLPKIYDP LSGPYRRRINL+L FSMLN                              
Sbjct: 501 KDYLPKIYDPLLSGPYRRRINLVLLFSMLNKSSRKNDSEKKKIWINKIHVIFFNTNYPQI 560

Query: 62  ------ETSRKNDITIPYKANASDRNSIDLTEISKKVPRWSYNLIKEIEQVYDITDGELV 121
                 E  RK+       A  S  NSI + EISKKVPRWSY LI E +Q+    D ++ 
Sbjct: 561 EDKIESEDRRKSFNFFFDGAKESGENSIAIKEISKKVPRWSYKLITETDQLIQENDEDMP 620

Query: 122 LDYQIRSRKCKRVVILTDLKKIKKISTSTSTPTSTPASTPASF 129
            D+QIRSR+ K V++  D  K+           + P +T  ++
Sbjct: 621 EDHQIRSRRAKNVILFNDRNKLTDRQKENEKIRNIPTNTNINY 663

[Close]
BLAST of Cucsa.253260 vs. TrEMBL
Match: A0A0D4BMZ2_9ROSI (Ycf1 (Fragment) OS=Trichosanthes sp. BOP010533 GN=ycf1 PE=4 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 7.8e-18
Identity = 63/163 (38.65%), Postives = 80/163 (49.08%), Query Frame = 1 

Query: 2   KEYLPKIYDPFLSGPYRRRINLLLGFSMLN------------------------------ 61
           K YLPK YDP LSGPYR++I+    FS +N                              
Sbjct: 84  KYYLPKTYDPLLSGPYRKQIHFFFSFSRINETSIKNDSENVWTNKIHAIFLNTNYPQIED 143

Query: 62  --ETSRK--------NDITIPYKANASDRNSIDLTEISKKVPRWSYNLIKEIEQVYDITD 121
             E+  +        + +  PY A AS +NSI L EISKKVPRWSY LI ++EQ     D
Sbjct: 144 KIESENRIKSLKFLFDAVITPYNAKASRQNSIGLREISKKVPRWSYKLIDDLEQQEGEND 203

Query: 122 GELVLDYQIRSRKCKRVVILTDLKKIKKISTSTSTPTSTPAST 125
             +  D++IRSRK KRVVI TD  +     T+T T T+ P  T
Sbjct: 204 ESMAEDHEIRSRKAKRVVIFTDNLETSDTYTNT-TDTNNPDQT 245

[Close]
BLAST of Cucsa.253260 vs. TrEMBL
Match: A0A0D4BM75_EUOAL (Ycf1 (Fragment) OS=Euonymus alatus GN=ycf1 PE=4 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 4.8e-15
Identity = 59/144 (40.97%), Postives = 74/144 (51.39%), Query Frame = 1 

Query: 3   EYLPKIYDPFLSGPYRRRINLLLGFSMLNET----------SRKNDI------------- 62
           EYLPKIYDP L+GP RR+I  L  F + NE           +R +DI             
Sbjct: 79  EYLPKIYDPLLNGPSRRKIKKLFLFPLRNEKKYIDPPWITINRIHDILFTTNFDQKQRID 138

Query: 63  TIPYKA------NASDRNSIDLTEISKKVPRWSYNLIKEIEQVYDITDGELVLDYQIRSR 118
           T   KA         DR  I + EISKKVPRWSY LI E+EQ     +   V DY+IRSR
Sbjct: 139 TFERKAFSNHEHRGEDRRKIGINEISKKVPRWSYKLINELEQQEREHEEISVKDYEIRSR 198

[Close]
BLAST of Cucsa.253260 vs. TrEMBL
Match: A0A0D4BMA9_9ROSI (Ycf1 (Fragment) OS=Euonymus sp. BOP010253 GN=ycf1 PE=4 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 8.1e-15
Identity = 59/144 (40.97%), Postives = 76/144 (52.78%), Query Frame = 1 

Query: 3   EYLPKIYDPFLSGPYRRRINLLLGFSMLNET----------SRKNDI------------- 62
           EYLPKIYDP L+GP RR+I  L  F + NE           +R +DI             
Sbjct: 82  EYLPKIYDPLLNGPSRRKIKKLFLFPLRNEKKYINPPWITINRIHDILFTTNFDQKQRID 141

Query: 63  TIPYKANAS------DRNSIDLTEISKKVPRWSYNLIKEIEQVYDITDGELVLDYQIRSR 118
           T   KA ++      DR  I + EISKKVPRWSY LI E+EQ     +   V DY+IRSR
Sbjct: 142 TFERKAFSNHEHRREDRRKIGINEISKKVPRWSYKLINELEQQEREHEEISVKDYEIRSR 201

[Close]
BLAST of Cucsa.253260 vs. TrEMBL
Match: A0A0D4BMM0_EUOMA (Ycf1 (Fragment) OS=Euonymus maackii GN=ycf1 PE=4 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 1.1e-14
Identity = 59/144 (40.97%), Postives = 76/144 (52.78%), Query Frame = 1 

Query: 3   EYLPKIYDPFLSGPYRRRINLLLGFSMLNET----------SRKNDI------------- 62
           EYLPKIYDP L+GP RR+I  LL   + NE           +R +DI             
Sbjct: 82  EYLPKIYDPLLNGPSRRKIKKLLLLPLRNEKKYINPPWITINRIHDILFTTNFDQKQRIE 141

Query: 63  TIPYKANAS------DRNSIDLTEISKKVPRWSYNLIKEIEQVYDITDGELVLDYQIRSR 118
           T   KA ++      DR  I + EISKKVPRWSY LI E+EQ     +   V DY+IRSR
Sbjct: 142 TFERKAFSNHEHRREDRRKIGINEISKKVPRWSYKLINELEQQEREHEEISVKDYEIRSR 201

[Close]
BLAST of Cucsa.253260 vs. TAIR10
Match: ATCG01130.1 (ATCG01130.1 Ycf1 protein)

HSP 1 Score: 59.3 bits (142), Expect = 2.0e-09
Identity = 29/64 (45.31%), Postives = 43/64 (67.19%), Query Frame = 1 

Query: 50  RNSIDLTEISKKVPRWSYNLIKEIEQVYDITDGELVLDYQIRSRKCKRVVILTDLKKIKK 109
           +N +D  EI+KKVPRWSY LI E+E++    +  + ++  IRSRK KRVV+ TD +   +
Sbjct: 592 KNFMDFPEINKKVPRWSYKLISELEELEGENEENVPMEPGIRSRKAKRVVVFTDKEPHGE 651

Query: 110 ISTS 114
           I T+
Sbjct: 652 IYTN 655


HSP 2 Score: 47.8 bits (112), Expect = 6.1e-06
Identity = 23/38 (60.53%), Postives = 27/38 (71.05%), Query Frame = 1 

Query: 2   KEYLPKIYDPFLSGPYRRRINLLLGFSMLNETSRKNDI 40
           KEYLPKIYDPFL G  R RI  L  F ++ ET RKN++
Sbjct: 478 KEYLPKIYDPFLHGISRGRIKKLPPFQIITETYRKNNL 515

[Close]
BLAST of Cucsa.253260 vs. NCBI nr
Match: gi|74027156|gb|AAZ94706.1| (hypothetical protein [Cucumis sativus])

HSP 1 Score: 219.2 bits (557), Expect = 4.4e-54
Identity = 117/149 (78.52%), Postives = 120/149 (80.54%), Query Frame = 1 

Query: 2   KEYLPKIYDPFLSGPYRRRINLLLGFSMLNETSRKND----------------------- 61
           KEYLPKIYDPFLSGPYRRRINLLLGFSMLNETSRKND                       
Sbjct: 455 KEYLPKIYDPFLSGPYRRRINLLLGFSMLNETSRKNDSENIWTNKIHAIIRNNNNGFFDG 514

Query: 62  ITIPYKANASDRNSIDLTEISKKVPRWSYNLIKEIEQVYDITDGELVLDYQIRSRKCKRV 121
           +TIPYKANASD+NSIDLTEISKKVPRWSY LIKEIEQVYDITDGELVLDYQIRSRKCKRV
Sbjct: 515 VTIPYKANASDQNSIDLTEISKKVPRWSYKLIKEIEQVYDITDGELVLDYQIRSRKCKRV 574

Query: 122 VILTDLKKIKKISTSTSTPTSTPASTPAS 128
           VIL+DL KIKKI  STSTPTSTP STP S
Sbjct: 575 VILSDLNKIKKI--STSTPTSTPTSTPTS 601

[Close]
BLAST of Cucsa.253260 vs. NCBI nr
Match: gi|74027156|gb|AAZ94706.1| (hypothetical protein [Cucumis sativus])

HSP 1 Score: 217.2 bits (552), Expect = 1.7e-53
Identity = 119/165 (72.12%), Postives = 122/165 (73.94%), Query Frame = 1 

Query: 2   KEYLPKIYDPFLSGPYRRRINLLLGFSMLNETSRKND----------------------- 61
           KEYLPKIYDPFLSGPYRRRINLLLGFSMLNETSRKND                       
Sbjct: 455 KEYLPKIYDPFLSGPYRRRINLLLGFSMLNETSRKNDSENIWTNKIHAIIRNNNNGFFDG 514

Query: 62  ITIPYKANASDRNSIDLTEISKKVPRWSYNLIKEIEQVYDITDGELVLDYQIRSRKCKRV 121
           +TIPYKANASD+NSIDLTEISKKVPRWSY LIKEIEQVYDITDGELVLDYQIRSRKCKRV
Sbjct: 515 VTIPYKANASDQNSIDLTEISKKVPRWSYKLIKEIEQVYDITDGELVLDYQIRSRKCKRV 574

Query: 122 VILTDLKKIKKISTST----------------STPTSTPASTPAS 128
           VIL+DL KIKKISTST                STPTSTP STP S
Sbjct: 575 VILSDLNKIKKISTSTPTSTPTSTPTSTSTPTSTPTSTPTSTPTS 619


HSP 2 Score: 27.7 bits (60), Expect = 1.9e+04
Identity = 13/17 (76.47%), Postives = 13/17 (76.47%), Query Frame = 1 

Query: 111 STSTSTPTSTPASTPAS 128
           STSTSTP S PAST  S
Sbjct: 619 STSTSTPASIPASTSTS 635


HSP 3 Score: 219.2 bits (557), Expect = 4.4e-54
Identity = 117/149 (78.52%), Postives = 120/149 (80.54%), Query Frame = 1 

Query: 2   KEYLPKIYDPFLSGPYRRRINLLLGFSMLNETSRKND----------------------- 61
           KEYLPKIYDPFLSGPYRRRINLLLGFSMLNETSRKND                       
Sbjct: 455 KEYLPKIYDPFLSGPYRRRINLLLGFSMLNETSRKNDSENIWTNKIHAIIRNNNNGFFDG 514

Query: 62  ITIPYKANASDRNSIDLTEISKKVPRWSYNLIKEIEQVYDITDGELVLDYQIRSRKCKRV 121
           +TIPYKANASD+NSIDLTEISKKVPRWSY LIKEIEQVYDITDGELVLDYQIRSRKCKRV
Sbjct: 515 VTIPYKANASDQNSIDLTEISKKVPRWSYKLIKEIEQVYDITDGELVLDYQIRSRKCKRV 574

Query: 122 VILTDLKKIKKISTSTSTPTSTPASTPAS 128
           VIL+DL KIKKI  STSTPTSTP STP S
Sbjct: 575 VILSDLNKIKKI--STSTPTSTPTSTPTS 601

[Close]
BLAST of Cucsa.253260 vs. NCBI nr
Match: gi|68164862|ref|YP_247658.1| (hypothetical protein CsCp113 [Cucumis sativus])

HSP 1 Score: 217.2 bits (552), Expect = 1.7e-53
Identity = 119/165 (72.12%), Postives = 122/165 (73.94%), Query Frame = 1 

Query: 2   KEYLPKIYDPFLSGPYRRRINLLLGFSMLNETSRKND----------------------- 61
           KEYLPKIYDPFLSGPYRRRINLLLGFSMLNETSRKND                       
Sbjct: 455 KEYLPKIYDPFLSGPYRRRINLLLGFSMLNETSRKNDSENIWTNKIHAIIRNNNNGFFDG 514

Query: 62  ITIPYKANASDRNSIDLTEISKKVPRWSYNLIKEIEQVYDITDGELVLDYQIRSRKCKRV 121
           +TIPYKANASD+NSIDLTEISKKVPRWSY LIKEIEQVYDITDGELVLDYQIRSRKCKRV
Sbjct: 515 VTIPYKANASDQNSIDLTEISKKVPRWSYKLIKEIEQVYDITDGELVLDYQIRSRKCKRV 574

Query: 122 VILTDLKKIKKISTST----------------STPTSTPASTPAS 128
           VIL+DL KIKKISTST                STPTSTP STP S
Sbjct: 575 VILSDLNKIKKISTSTPTSTPTSTPTSTSTPTSTPTSTPTSTPTS 619


HSP 2 Score: 29.6 bits (65), Expect = 4.9e+03
Identity = 15/20 (75.00%), Postives = 15/20 (75.00%), Query Frame = 1 

Query: 111 STSTSTPTSTPASTPASFFI 131
           STSTST TS PAST  S FI
Sbjct: 639 STSTSTSTSIPASTSTSTFI 658


HSP 3 Score: 27.7 bits (60), Expect = 1.9e+04
Identity = 13/17 (76.47%), Postives = 13/17 (76.47%), Query Frame = 1 

Query: 111 STSTSTPTSTPASTPAS 128
           STSTSTP S PAST  S
Sbjct: 619 STSTSTPASIPASTSTS 635


HSP 4 Score: 219.2 bits (557), Expect = 4.4e-54
Identity = 117/149 (78.52%), Postives = 120/149 (80.54%), Query Frame = 1 

Query: 2   KEYLPKIYDPFLSGPYRRRINLLLGFSMLNETSRKND----------------------- 61
           KEYLPKIYDPFLSGPYRRRINLLLGFSMLNETSRKND                       
Sbjct: 455 KEYLPKIYDPFLSGPYRRRINLLLGFSMLNETSRKNDSENIWTNKIHAIIRNNNNGFFDG 514

Query: 62  ITIPYKANASDRNSIDLTEISKKVPRWSYNLIKEIEQVYDITDGELVLDYQIRSRKCKRV 121
           +TIPYKANASD+NSIDLTEISKKVPRWSY LIKEIEQVYDITDGELVLDYQIRSRKCKRV
Sbjct: 515 VTIPYKANASDQNSIDLTEISKKVPRWSYKLIKEIEQVYDITDGELVLDYQIRSRKCKRV 574

Query: 122 VILTDLKKIKKISTSTSTPTSTPASTPAS 128
           VIL+DL KIKKI  STSTPTSTP STP S
Sbjct: 575 VILSDLNKIKKI--STSTPTSTPTSTPTS 601

[Close]
BLAST of Cucsa.253260 vs. NCBI nr
Match: gi|158518591|sp|Q4VZL0.2|TI214_CUCSA (RecName: Full=Protein TIC 214; AltName: Full=Translocon at the inner envelope membrane of chloroplasts 214; Short=AtTIC214)

HSP 1 Score: 217.2 bits (552), Expect = 1.7e-53
Identity = 119/165 (72.12%), Postives = 122/165 (73.94%), Query Frame = 1 

Query: 2   KEYLPKIYDPFLSGPYRRRINLLLGFSMLNETSRKND----------------------- 61
           KEYLPKIYDPFLSGPYRRRINLLLGFSMLNETSRKND                       
Sbjct: 455 KEYLPKIYDPFLSGPYRRRINLLLGFSMLNETSRKNDSENIWTNKIHAIIRNNNNGFFDG 514

Query: 62  ITIPYKANASDRNSIDLTEISKKVPRWSYNLIKEIEQVYDITDGELVLDYQIRSRKCKRV 121
           +TIPYKANASD+NSIDLTEISKKVPRWSY LIKEIEQVYDITDGELVLDYQIRSRKCKRV
Sbjct: 515 VTIPYKANASDQNSIDLTEISKKVPRWSYKLIKEIEQVYDITDGELVLDYQIRSRKCKRV 574

Query: 122 VILTDLKKIKKISTST----------------STPTSTPASTPAS 128
           VIL+DL KIKKISTST                STPTSTP STP S
Sbjct: 575 VILSDLNKIKKISTSTPTSTPTSTPTSTSTPTSTPTSTPTSTPTS 619


HSP 2 Score: 27.7 bits (60), Expect = 1.9e+04
Identity = 13/17 (76.47%), Postives = 13/17 (76.47%), Query Frame = 1 

Query: 111 STSTSTPTSTPASTPAS 128
           STSTSTP S PAST  S
Sbjct: 619 STSTSTPASIPASTSTS 635


HSP 3 Score: 99.4 bits (246), Expect = 5.0e-18
Identity = 62/163 (38.04%), Postives = 79/163 (48.47%), Query Frame = 1 

Query: 2   KEYLPKIYDPFLSGPYRRRINLLLGFSMLN------------------------------ 61
           K+YLPKIYDP LSGPYRRRINL+L FSMLN                              
Sbjct: 501 KDYLPKIYDPLLSGPYRRRINLVLLFSMLNKSSRKNDSEKKKIWINKIHVIFFNTNYPQI 560

Query: 62  ------ETSRKNDITIPYKANASDRNSIDLTEISKKVPRWSYNLIKEIEQVYDITDGELV 121
                 E  RK+       A  S  NSI + EISKKVPRWSY LI E +Q+    D ++ 
Sbjct: 561 EDKIESEDRRKSFNFFFDGAKESGENSIAIKEISKKVPRWSYKLITETDQLIQENDEDMP 620

Query: 122 LDYQIRSRKCKRVVILTDLKKIKKISTSTSTPTSTPASTPASF 129
            D+QIRSR+ K V++  D  K+           + P +T  ++
Sbjct: 621 EDHQIRSRRAKNVILFNDRNKLTDRQKENEKIRNIPTNTNINY 663

[Close]
BLAST of Cucsa.253260 vs. NCBI nr
Match: gi|766946567|gb|AJT37044.1| (Ycf1, partial (chloroplast) [Trichosanthes sp. BOP010533])

HSP 1 Score: 98.2 bits (243), Expect = 1.1e-17
Identity = 63/163 (38.65%), Postives = 80/163 (49.08%), Query Frame = 1 

Query: 2   KEYLPKIYDPFLSGPYRRRINLLLGFSMLN------------------------------ 61
           K YLPK YDP LSGPYR++I+    FS +N                              
Sbjct: 84  KYYLPKTYDPLLSGPYRKQIHFFFSFSRINETSIKNDSENVWTNKIHAIFLNTNYPQIED 143

Query: 62  --ETSRK--------NDITIPYKANASDRNSIDLTEISKKVPRWSYNLIKEIEQVYDITD 121
             E+  +        + +  PY A AS +NSI L EISKKVPRWSY LI ++EQ     D
Sbjct: 144 KIESENRIKSLKFLFDAVITPYNAKASRQNSIGLREISKKVPRWSYKLIDDLEQQEGEND 203

Query: 122 GELVLDYQIRSRKCKRVVILTDLKKIKKISTSTSTPTSTPAST 125
             +  D++IRSRK KRVVI TD  +     T+T T T+ P  T
Sbjct: 204 ESMAEDHEIRSRKAKRVVIFTDNLETSDTYTNT-TDTNNPDQT 245

The following BLAST results are available for this feature:
BLAST of Cucsa.253260 vs. Swiss-Prot
Match NameE-valueIdentityDescription
TI214_CUCSA2.7e-5678.52Protein TIC 214 OS=Cucumis sativus GN=TIC214 PE=3 SV=2[more]
TI214_POPTR1.9e-1233.33Protein TIC 214 OS=Populus trichocarpa GN=TIC214 PE=3 SV=1[more]
TI214_POPAL1.9e-1233.33Protein TIC 214 OS=Populus alba GN=TIC214 PE=3 SV=1[more]
TI214_RANMC1.5e-0937.70Protein TIC 214 OS=Ranunculus macranthus GN=TIC214 PE=3 SV=2[more]
TI214_NANDO1.9e-0942.39Protein TIC 214 OS=Nandina domestica GN=TIC214 PE=3 SV=1[more]
BLAST of Cucsa.253260 vs. TrEMBL
Match NameE-valueIdentityDescription
G3ETV7_CUCME3.5e-1838.04Hypothetical chloroplast RF19 OS=Cucumis melo subsp. melo GN=ycf1 PE=4 SV=1[more]
A0A0D4BMZ2_9ROSI7.8e-1838.65Ycf1 (Fragment) OS=Trichosanthes sp. BOP010533 GN=ycf1 PE=4 SV=1[more]
A0A0D4BM75_EUOAL4.8e-1540.97Ycf1 (Fragment) OS=Euonymus alatus GN=ycf1 PE=4 SV=1[more]
A0A0D4BMA9_9ROSI8.1e-1540.97Ycf1 (Fragment) OS=Euonymus sp. BOP010253 GN=ycf1 PE=4 SV=1[more]
A0A0D4BMM0_EUOMA1.1e-1440.97Ycf1 (Fragment) OS=Euonymus maackii GN=ycf1 PE=4 SV=1[more]
BLAST of Cucsa.253260 vs. TAIR10
Match NameE-valueIdentityDescription
ATCG01130.12.0e-0945.31ATCG01130.1 Ycf1 protein[more]
BLAST of Cucsa.253260 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|74027156|gb|AAZ94706.1|4.4e-5478.52hypothetical protein [Cucumis sativus][more]
gi|74027156|gb|AAZ94706.1|1.7e-5372.12hypothetical protein [Cucumis sativus][more]
gi|68164862|ref|YP_247658.1|1.7e-5372.12hypothetical protein CsCp113 [Cucumis sativus][more]
gi|158518591|sp|Q4VZL0.2|TI214_CUCSA1.7e-5372.12RecName: Full=Protein TIC 214; AltName: Full=Translocon at the inner envelope me... [more]
gi|766946567|gb|AJT37044.1|1.1e-1738.65Ycf1, partial (chloroplast) [Trichosanthes sp. BOP010533][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR008896TIC214
Vocabulary: Cellular Component
TermDefinition
GO:0016021integral component of membrane
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015031 protein transport
cellular_component GO:0009706 chloroplast inner membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0003674 molecular_function
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.253260.1Cucsa.253260.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008896Protein TIC214PFAMPF05758Ycf1coord: 44..115
score: 1.0E-17coord: 2..39
score: 2.
NoneNo IPR availablePANTHERPTHR33163FAMILY NOT NAMEDcoord: 50..114
score: 6.9
NoneNo IPR availablePANTHERPTHR33163:SF3MEMBRANE PROTEIN YCF1-RELATEDcoord: 50..114
score: 6.9

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cucsa.253260CSPI03G14240Wild cucumber (PI 183967)cgycpiB383
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cucsa.253260Bottle gourd (USVL1VR-Ls)cgylsiB412
Cucsa.253260Bottle gourd (USVL1VR-Ls)cgylsiB420
Cucsa.253260Melon (DHL92) v3.6.1cgymedB425
Cucsa.253260Melon (DHL92) v3.6.1cgymedB432
Cucsa.253260Silver-seed gourdcarcgyB0083
Cucsa.253260Silver-seed gourdcarcgyB0133
Cucsa.253260Silver-seed gourdcarcgyB0681
Cucsa.253260Silver-seed gourdcarcgyB0788
Cucsa.253260Silver-seed gourdcarcgyB0960
Cucsa.253260Cucumber (Chinese Long) v3cgycucB387
Cucsa.253260Cucumber (Chinese Long) v3cgycucB397
Cucsa.253260Watermelon (97103) v2cgywmbB452
Cucsa.253260Watermelon (97103) v2cgywmbB453
Cucsa.253260Wax gourdcgywgoB505
Cucsa.253260Wax gourdcgywgoB522
Cucsa.253260Cucumber (Gy14) v1cgycgyB108
Cucsa.253260Cucurbita maxima (Rimu)cgycmaB0713
Cucsa.253260Cucurbita maxima (Rimu)cgycmaB0714
Cucsa.253260Cucurbita maxima (Rimu)cgycmaB0709
Cucsa.253260Cucurbita maxima (Rimu)cgycmaB0727
Cucsa.253260Cucurbita maxima (Rimu)cgycmaB0731
Cucsa.253260Cucurbita moschata (Rifu)cgycmoB0700
Cucsa.253260Cucurbita moschata (Rifu)cgycmoB0707
Cucsa.253260Cucurbita moschata (Rifu)cgycmoB0718
Cucsa.253260Cucurbita moschata (Rifu)cgycmoB0720
Cucsa.253260Wild cucumber (PI 183967)cgycpiB392
Cucsa.253260Cucumber (Chinese Long) v2cgycuB366
Cucsa.253260Cucumber (Chinese Long) v2cgycuB373
Cucsa.253260Melon (DHL92) v3.5.1cgymeB430
Cucsa.253260Melon (DHL92) v3.5.1cgymeB439
Cucsa.253260Watermelon (Charleston Gray)cgywcgB451
Cucsa.253260Watermelon (Charleston Gray)cgywcgB453
Cucsa.253260Watermelon (97103) v1cgywmB479
Cucsa.253260Watermelon (97103) v1cgywmB483
Cucsa.253260Cucurbita pepo (Zucchini)cgycpeB0669
Cucsa.253260Cucurbita pepo (Zucchini)cgycpeB0671
Cucsa.253260Cucurbita pepo (Zucchini)cgycpeB0678
Cucsa.253260Cucurbita pepo (Zucchini)cgycpeB0682
Cucsa.253260Cucurbita pepo (Zucchini)cgycpeB0687