Cucsa.253070 (gene) Cucumber (Gy14) v1

Overview
NameCucsa.253070
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionIsoleucine--tRNA ligase
Locationscaffold02229 : 1822007 .. 1824022 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CGCTATGGAGTATGGGCAGATTGAAACAATCCATCGTTTAACTCTCAGTCCAGAGTATGAAGCTGTCCAGGTAACTATTGCTTGCATTCTCATGTTCTACTTTCTATGTGGTTCCCTTAAGCAGTTTGTTGCAAATTTCAATTCAACAGTTTTGTGTTTGTATTTTTTGTATGTTAGATTGAGGTGTTTGGTCAAATGGCCATCCAAGGTTATATATTTAGAGGGAGAAAGCCAGTTCACTGGAGTCCCTCATCACGAACTGCTCTTGCAGAGGCTGAATTGGAGGTAAATCATTTGTTGAAGCATGAGAACACAATCAAATTAGATAAACTTATTACAGATATTCTCTGGTGTAAAGAGAAAATCATTTAGGGTTTTTTTTTAAAGAATAAAAAAATCTAGCATGGTTTTTCTATGAAACCTATTGCTTTCAAAACATTAAATCACACGGATAATCATTTTCCATGATGAATTTTGTACTCTAATCTATTTTTCATGCTGATTCCTGTCATTTGCTTAAATTTGGTTATATCATTTTTTTCCTGCCCTACCTGTTTGATAATTGCATTCAGTACCCTGAGGGGCATACTTCAAGAAGCATATACGCCATTTTTAGATTGGTTAAGGAAGGTCCATCTTCAGGCGGTGTGCTACAAGATTTTTTCCGAGACTTGTGCTTGGCCATATGGACTACAACTCCTTGGACTATTCCAGCCAATGCTGGTGAGCTTTTGTTCTATTACAATGGGAGCAATATGGTTGTAGATTTATTTCCGTATGTTTCCAAGTAATATTATAATTTTTTTTTACTCTTTTCTGATGATAAATACAATCCCTTTAGAAAATGGAGAAAGGTTTACTGAAATAGAAAGAGGAACGGTTGGTCTACCAACTACTAAATAAGAAGTGAACAACAAATATGTCTTGTTGTCCTAATCCCAAGCAAGAAAAATTATGTTTTCTTGTTTATAAACCTCCAAGGTTCCAACCCATATATTCGACTCTTACATGTTTTTGTTTTTCTAAAAAAATATCTTATAAGCATCTGACCTCGGTAGTGCTCAACTTGCCTGTTCTCATAGAATAGCTGCATGACCTACTTCATTTGGACTCTTCCATGGTTTTCGATGATAATTTTTTAGCAAAAAGTTATTCTCTTCTAAAACTTGAGCTATCTATCTTTACGTTGATATGCATTTTAACTTTTTCCCACTTTTTCTCACAGTATGCTTGTATTTTCCATAATTGAATTGCTGGCACAGTCTGAAGATGTCTTTGTTTTGATTGGAAATAAAAAGAAAAGGCCTGGTAATATTCCGAAGGAGGGAAAGAGGACTTTCCTTCTAGTAGCAACAGATCTTGTGCCAACTTTTGGAAGCAAAATGGGGTGTGAAGCTTGTTGTCAAGAAGAAATTGTTGGGTTCAGAACTTGAAAACTGCAGGTTTTATTTCTTTCCCTAAACAATCAGAAGGAACAGTGAAAGTGGGAGCTTCCTTATCAAGTGATTTTTCTTCCTAAAAAAACTTGTAGGTTTCATATTCGGTTAGAAGAAATCTAGAAATGTTGGTAAATTTTAACATATTGTTTTACCAAGCCTATCTCTTAGTAATTTACCTTAGTCACTTGATGTGAAAGTGAATATGTGAACTAAGGGTGATATATAATTAAATTTGGCTTCAACCACAAACTTAAGCTTTTGGGTGAATTGACGGTTTAATATGGTATTAGAACAGGAGGTCCTGTGTTGTTCAAGCTCCTGTATTGTTGTTTCCTTCCCAATTAAAATCGATTTCCACTTTTTGGGCCTTTCAAATATTTCAAAGCCACAAGTGAGGGAGAGTGTTGGTGATATATAATTAAATTTGTCTTCAACTACAAACTTAAGCTTTGGGTGAATTGACGAATTAATATTAAGTATTATTAAACCAATTGTTTTCTTCAGCTACATCCACCCAAATGGTAATAGGGTGTGCCAAGTTGTTATTGGAGGAGATTACCAGCAAGGTTATAATTAG

mRNA sequence

CGCTATGGAGTATGGGCAGATTGAAACAATCCATCGTTTAACTCTCAGTCCAGAGTATGAAGCTGTCCAGATTGAGGTGTTTGGTCAAATGGCCATCCAAGGTTATATATTTAGAGGGAGAAAGCCAGTTCACTGGAGTCCCTCATCACGAACTGCTCTTGCAGAGGCTGAATTGGAGTACCCTGAGGGGCATACTTCAAGAAGCATATACGCCATTTTTAGATTGGTTAAGGAAGGTCCATCTTCAGGCGGTGTGCTACAAGATTTTTTCCGAGACTTGTGCTTGGCCATATGGACTACAACTCCTTGGACTATTCCAGCCAATGCTGGCCTGCAACAGATCTTGTGCCAACTTTTGGAAGCAAAATGGGGTGTGAAGCTTGTTGTCAAGAAGAAATTGTTGGGTTCAGAACTTGAAAACTGCAGCTACATCCACCCAAATGGTAATAGGGTGTGCCAAGTTGTTATTGGAGGAGATTACCAGCAAGGTTATAATTAG

Coding sequence (CDS)

CGCTATGGAGTATGGGCAGATTGAAACAATCCATCGTTTAACTCTCAGTCCAGAGTATGAAGCTGTCCAGATTGAGGTGTTTGGTCAAATGGCCATCCAAGGTTATATATTTAGAGGGAGAAAGCCAGTTCACTGGAGTCCCTCATCACGAACTGCTCTTGCAGAGGCTGAATTGGAGTACCCTGAGGGGCATACTTCAAGAAGCATATACGCCATTTTTAGATTGGTTAAGGAAGGTCCATCTTCAGGCGGTGTGCTACAAGATTTTTTCCGAGACTTGTGCTTGGCCATATGGACTACAACTCCTTGGACTATTCCAGCCAATGCTGGCCTGCAACAGATCTTGTGCCAACTTTTGGAAGCAAAATGGGGTGTGAAGCTTGTTGTCAAGAAGAAATTGTTGGGTTCAGAACTTGAAAACTGCAGCTACATCCACCCAAATGGTAATAGGGTGTGCCAAGTTGTTATTGGAGGAGATTACCAGCAAGGTTATAATTAG

Protein sequence

AMEYGQIETIHRLTLSPEYEAVQIEVFGQMAIQGYIFRGRKPVHWSPSSRTALAEAELEYPEGHTSRSIYAIFRLVKEGPSSGGVLQDFFRDLCLAIWTTTPWTIPANAGLQQILCQLLEAKWGVKLVVKKKLLGSELENCSYIHPNGNRVCQVVIGGDYQQGYN*
Homology
[Close]
BLAST of Cucsa.253070 vs. Swiss-Prot
Match: SYI_SYNY3 (Isoleucine--tRNA ligase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) GN=ileS PE=3 SV=1)

HSP 1 Score: 170.2 bits (430), Expect = 1.8e-41
Identity = 90/173 (52.02%), Postives = 106/173 (61.27%), Query Frame = 1 

Query: 13  LTLSPEYEAVQIEVFGQMAIQGYIFRGRKPVHWSPSSRTALAEAELEYPEGHTSRSIYAI 72
           LTL+PEYEA QI VFG MA++GYI+RG KPVHWSPSSRTALAEAELEYPEGHTSRSIY  
Sbjct: 158 LTLTPEYEAAQIGVFGAMALKGYIYRGLKPVHWSPSSRTALAEAELEYPEGHTSRSIYVS 217

Query: 73  FRLVKEGPSSGGVLQDFFRDLCLAIWTTTPWTIPANAGLQQI------------------ 132
           F + + G  +  +L  +  DL +AIWTTTPWT+P N  +                     
Sbjct: 218 FPITQAGEKAAEILAPYIDDLAVAIWTTTPWTLPGNLAVALNPELTYAVVETESHIFHRS 277

Query: 133 -------LCQLLEAKWGVKLVVKKKLLGSELENCSYIHPNGNRVCQVVIGGDY 161
                  L + L   +GVKL VK  L G  LEN  Y HP  +RV  +VIGGDY
Sbjct: 278 YLIVALDLVEKLSETFGVKLTVKVTLQGESLENTCYQHPLFDRVSPIVIGGDY 330

[Close]
BLAST of Cucsa.253070 vs. Swiss-Prot
Match: SYIM_ARATH (Isoleucine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana GN=OVA2 PE=2 SV=1)

HSP 1 Score: 169.1 bits (427), Expect = 4.1e-41
Identity = 97/193 (50.26%), Postives = 114/193 (59.07%), Query Frame = 1 

Query: 13  LTLSPEYEAVQIEVFGQMAIQGYIFRGRKPVHWSPSSRTALAEAELEYPEGHTSRSIYAI 72
           LTL PEYEA QIEVFGQMA++GYI+RGRKPVHWSPSSRTALAEAELEYPEGH S+SIYAI
Sbjct: 253 LTLDPEYEAAQIEVFGQMALKGYIYRGRKPVHWSPSSRTALAEAELEYPEGHISKSIYAI 312

Query: 73  FRLVKEGPSSGGVLQDFFRDLCLAIWTTTPWTIPANAG------LQQILCQLLEAKWGVK 132
           F+LV  G +   +L +F  ++ LA+WTTTPWT+PANA       LQ  + ++        
Sbjct: 313 FKLV--GGAKTSLLDEFIPNIYLAVWTTTPWTMPANAAVAVNAKLQYSVVEVQSFSEDES 372

Query: 133 LVV--KKKLLG-------------------------------------SELENCSYIHPN 161
            V   KKK+ G                                     S+LENC Y HP 
Sbjct: 373 TVTSNKKKIPGKVLKNQQKLFVIVATDLVPALEAKWGVKLSISKTFLGSDLENCRYTHPI 432

[Close]
BLAST of Cucsa.253070 vs. Swiss-Prot
Match: SYI_CYAP7 (Isoleucine--tRNA ligase OS=Cyanothece sp. (strain PCC 7424) GN=ileS PE=3 SV=1)

HSP 1 Score: 164.9 bits (416), Expect = 7.8e-40
Identity = 85/173 (49.13%), Postives = 109/173 (63.01%), Query Frame = 1 

Query: 13  LTLSPEYEAVQIEVFGQMAIQGYIFRGRKPVHWSPSSRTALAEAELEYPEGHTSRSIYAI 72
           LTL+P+YEA QI VFGQMA++GYI+RG KPVHWSPSS+TALAEAELEYPEGHTSRS+YA+
Sbjct: 158 LTLTPDYEAAQIGVFGQMALKGYIYRGLKPVHWSPSSQTALAEAELEYPEGHTSRSVYAV 217

Query: 73  FRLVKEGPSSGGVLQDFFRDLCLAIWTTTPWTIPANAG---------------------- 132
           F + K   ++  VL  + +DL +AIWTTTPWT+P N                        
Sbjct: 218 FPITKASDTAKKVLTPYLKDLGVAIWTTTPWTLPGNLAVALNPDLTYAVVESGKPVCKHQ 277

Query: 133 ---LQQILCQLLEAKWGVKLVVKKKLLGSELENCSYIHPNGNRVCQVVIGGDY 161
              +   L   L A +   L VK  L G +LE+ +Y HP  +R  +++IGGDY
Sbjct: 278 YFIVAADLVDKLSATFETPLTVKATLKGEDLEHTTYRHPLFDRESEILIGGDY 330

[Close]
BLAST of Cucsa.253070 vs. Swiss-Prot
Match: SYI_SYNP6 (Isoleucine--tRNA ligase OS=Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) GN=ileS PE=3 SV=1)

HSP 1 Score: 155.2 bits (391), Expect = 6.1e-37
Identity = 88/168 (52.38%), Postives = 108/168 (64.29%), Query Frame = 1 

Query: 13  LTLSPEYEAVQIEVFGQMAIQGYIFRGRKPVHWSPSSRTALAEAELEYPEGHTSRSIYAI 72
           LTL+PEYEA QI+VFGQM ++GYI+RG KPVHWSPSSRTALAEAELEYP+GHTSRSIY  
Sbjct: 158 LTLTPEYEAAQIDVFGQMVLKGYIYRGLKPVHWSPSSRTALAEAELEYPDGHTSRSIYVA 217

Query: 73  FRLVKEGPSSGGVLQDFFRDLCLAIWTTTPWTIPAN-----------AGLQQILCQLLEA 132
             +V+   ++  +L + + +L LAIWTTTPWTIPAN           A +Q   C L+ A
Sbjct: 218 MPIVQLSEAAQPLLGN-YANLALAIWTTTPWTIPANLAVAVNGELTYAVVQAGDCHLIVA 277

Query: 133 ---------KWGVKLVVKKKLLGSELENCSYIHPNGNRVCQVVIGGDY 161
                     +  +L V     GS LE+  Y HP  +R   VVIGGDY
Sbjct: 278 AELAESLSKTFATELTVLATFPGSVLEHSRYRHPLYDRESPVVIGGDY 324

[Close]
BLAST of Cucsa.253070 vs. Swiss-Prot
Match: SYI_NOSP7 (Isoleucine--tRNA ligase OS=Nostoc punctiforme (strain ATCC 29133 / PCC 73102) GN=ileS PE=3 SV=1)

HSP 1 Score: 154.1 bits (388), Expect = 1.4e-36
Identity = 82/175 (46.86%), Postives = 105/175 (60.00%), Query Frame = 1 

Query: 13  LTLSPEYEAVQIEVFGQMAIQGYIFRGRKPVHWSPSSRTALAEAELEYPEGHTSRSIYAI 72
           LTL PEYEA QI VFGQM ++GYI+RG KPVHWSPSS+TALAEAELEYPEGH SRSIYA 
Sbjct: 158 LTLKPEYEAAQIGVFGQMFLKGYIYRGLKPVHWSPSSKTALAEAELEYPEGHVSRSIYAA 217

Query: 73  FRLVKEGPSSGGVLQDFFRDLCLAIWTTTPWTIPANAG---------------------- 132
           F +     +   +L ++  DL +AIWTTTPWTIP N                        
Sbjct: 218 FPVTSLAEAVKPLLAEYQSDLGVAIWTTTPWTIPGNLAVAVNADLNYAVVEVSQSEAQSK 277

Query: 133 -----LQQILCQLLEAKWGVKLVVKKKLLGSELENCSYIHPNGNRVCQVVIGGDY 161
                +   L + L +  G++L +K    G++LE+ +Y HP  +R   +V+GGDY
Sbjct: 278 FKYLIVAADLVERLSSTLGLELTLKATFKGNDLEHTTYRHPLFDRESPIVVGGDY 332

[Close]
BLAST of Cucsa.253070 vs. TrEMBL
Match: M0TSR7_MUSAM (Uncharacterized protein OS=Musa acuminata subsp. malaccensis PE=3 SV=1)

HSP 1 Score: 216.5 bits (550), Expect = 2.5e-53
Identity = 106/165 (64.24%), Postives = 125/165 (75.76%), Query Frame = 1 

Query: 13  LTLSPEYEAVQIEVFGQMAIQGYIFRGRKPVHWSPSSRTALAEAELEYPEGHTSRSIYAI 72
           LTLSPEYEA QIEVFGQMA++GYI+RGRKPVHWSPSS TALAEAELEYPEGH SRSIYA 
Sbjct: 227 LTLSPEYEAAQIEVFGQMALRGYIYRGRKPVHWSPSSCTALAEAELEYPEGHISRSIYAT 286

Query: 73  FRLVKEGPSSGGVLQDFFRDLCLAIWTTTPWTIPANAG------LQQILCQ--------- 132
           F+++   P S  +L++FF +LCLAIWTTTPWTIPANA       L+ ++ +         
Sbjct: 287 FKIISAPPPSCHLLEEFFPELCLAIWTTTPWTIPANAAVAVNAKLEYVVAEPSVIVASEL 346

Query: 133 --LLEAKWGVKLVVKKKLLGSELENCSYIHPNGNRVCQVVIGGDY 161
             +LE+KWGVKLVVKK  LGS LENC Y+HP   + C VV+GGDY
Sbjct: 347 VPMLESKWGVKLVVKKAFLGSTLENCRYVHPLNGKECPVVLGGDY 391

[Close]
BLAST of Cucsa.253070 vs. TrEMBL
Match: B9RT52_RICCO (Isoleucyl tRNA synthetase, putative OS=Ricinus communis GN=RCOM_0681070 PE=3 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 3.4e-50
Identity = 109/192 (56.77%), Postives = 124/192 (64.58%), Query Frame = 1 

Query: 13  LTLSPEYEAVQIEVFGQMAIQGYIFRGRKPVHWSPSSRTALAEAELEYPEGHTSRSIYAI 72
           LTL P+YEA QIEVFGQMA+QGYI+RGRKPVHWSPSS TALAEAELEYPEGH S+SIYAI
Sbjct: 264 LTLDPDYEAAQIEVFGQMALQGYIYRGRKPVHWSPSSHTALAEAELEYPEGHVSKSIYAI 323

Query: 73  FRLVKEGPSSGGVLQDFFRDLCLAIWTTTPWTIPANAG------LQQILCQL-------- 132
           FR+    P+S  +L++FF DL LAIWTTTPWT+PANA       LQ  + ++        
Sbjct: 324 FRVASAPPTSRLLLEEFFPDLFLAIWTTTPWTVPANAAVAVNSKLQYAVVEVQSLEDAST 383

Query: 133 ------------------------------LEAKWGVKLVVKKKLLGSELENCSYIHPNG 161
                                         LEAKW VKLV+KK L GS+LENC YIHP  
Sbjct: 384 SPGNKKRRFGNVLREQKKLFLIVASDLMPTLEAKWSVKLVIKKTLSGSDLENCRYIHPID 443

[Close]
BLAST of Cucsa.253070 vs. TrEMBL
Match: A0A068TRK4_COFCA (Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00025819001 PE=4 SV=1)

HSP 1 Score: 204.5 bits (519), Expect = 9.8e-50
Identity = 109/193 (56.48%), Postives = 126/193 (65.28%), Query Frame = 1 

Query: 13  LTLSPEYEAVQIEVFGQMAIQGYIFRGRKPVHWSPSSRTALAEAELEYPEGHTSRSIYAI 72
           LTL PEYEA QIEVFG+MAIQG+I+RGRKPVHWSPSSRTALAEAELEYPEGH S S+YAI
Sbjct: 93  LTLDPEYEAAQIEVFGKMAIQGFIYRGRKPVHWSPSSRTALAEAELEYPEGHVSNSMYAI 152

Query: 73  FRLVKEGPSSGGVLQDFFRDLCLAIWTTTPWTIPANAG------LQQILCQLL------- 132
           F+LV   P+   +L++FF +LCLAIWTTTPWTIPANA       LQ  + ++        
Sbjct: 153 FKLV-NAPNLCNLLEEFFPNLCLAIWTTTPWTIPANAAVAVNPKLQYAIVEVQSGSTDSS 212

Query: 133 --------------------------------EAKWGVKLVVKKKLLGSELENCSYIHPN 161
                                           EAKWG KL++KKKLLGS+LENCSY+HP 
Sbjct: 213 TLSGNAKKKLGNVFEEYEKLFFIVALDLVPTLEAKWGKKLLIKKKLLGSDLENCSYVHPI 272

[Close]
BLAST of Cucsa.253070 vs. TrEMBL
Match: A5ALP9_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_015862 PE=3 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 5.1e-46
Identity = 109/193 (56.48%), Postives = 120/193 (62.18%), Query Frame = 1 

Query: 13  LTLSPEYEAVQIEVFGQMAIQGYIFRGRKPVHWSPSSRTALAEAELEYPEGHTSRSIYAI 72
           LTL PEYEA QIEVFGQMA+QGYI+RGRKPVHWSPSSRTALAEAELEYPEGH S+SIYAI
Sbjct: 218 LTLDPEYEASQIEVFGQMALQGYIYRGRKPVHWSPSSRTALAEAELEYPEGHISKSIYAI 277

Query: 73  FRLVKEGPSSGGVLQDFFRDLCLAIWTTTPWTIPANAG-----------------LQQI- 132
           FRLV    +SG  L+DF  DLCLAIWTTTPWTIPANA                  L+ + 
Sbjct: 278 FRLVSAPVTSGTSLEDFLPDLCLAIWTTTPWTIPANAAVAVNAKLQYSVVEVHSPLEDVS 337

Query: 133 ---------LCQLLEAKWGVKLVVK------------------KKLLGSELENCSYIHPN 161
                    L   L+ +  + LVV                   K LLGS+LENC YIHP 
Sbjct: 338 KSKQNEKGRLGNALKEQKNLFLVVASDLVPTLEAKWGLKLVIKKTLLGSDLENCRYIHPI 397

[Close]
BLAST of Cucsa.253070 vs. TrEMBL
Match: F6HDF8_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_05s0020g01550 PE=3 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 5.1e-46
Identity = 109/193 (56.48%), Postives = 120/193 (62.18%), Query Frame = 1 

Query: 13  LTLSPEYEAVQIEVFGQMAIQGYIFRGRKPVHWSPSSRTALAEAELEYPEGHTSRSIYAI 72
           LTL PEYEA QIEVFGQMA+QGYI+RGRKPVHWSPSSRTALAEAELEYPEGH S+SIYAI
Sbjct: 256 LTLDPEYEASQIEVFGQMALQGYIYRGRKPVHWSPSSRTALAEAELEYPEGHISKSIYAI 315

Query: 73  FRLVKEGPSSGGVLQDFFRDLCLAIWTTTPWTIPANAG-----------------LQQI- 132
           FRLV    +SG  L+DF  DLCLAIWTTTPWTIPANA                  L+ + 
Sbjct: 316 FRLVSAPVTSGTSLEDFLPDLCLAIWTTTPWTIPANAAVAVNAKLQYSVVEVHSPLEDVS 375

Query: 133 ---------LCQLLEAKWGVKLVVK------------------KKLLGSELENCSYIHPN 161
                    L   L+ +  + LVV                   K LLGS+LENC YIHP 
Sbjct: 376 KSKQNEKGRLGNALKEQKNLFLVVASDLVPTLEAKWGLKLVIKKTLLGSDLENCRYIHPI 435

[Close]
BLAST of Cucsa.253070 vs. TAIR10
Match: AT5G49030.1 (AT5G49030.1 tRNA synthetase class I (I, L, M and V) family protein)

HSP 1 Score: 169.1 bits (427), Expect = 2.3e-42
Identity = 97/193 (50.26%), Postives = 114/193 (59.07%), Query Frame = 1 

Query: 13  LTLSPEYEAVQIEVFGQMAIQGYIFRGRKPVHWSPSSRTALAEAELEYPEGHTSRSIYAI 72
           LTL PEYEA QIEVFGQMA++GYI+RGRKPVHWSPSSRTALAEAELEYPEGH S+SIYAI
Sbjct: 253 LTLDPEYEAAQIEVFGQMALKGYIYRGRKPVHWSPSSRTALAEAELEYPEGHISKSIYAI 312

Query: 73  FRLVKEGPSSGGVLQDFFRDLCLAIWTTTPWTIPANAG------LQQILCQLLEAKWGVK 132
           F+LV  G +   +L +F  ++ LA+WTTTPWT+PANA       LQ  + ++        
Sbjct: 313 FKLV--GGAKTSLLDEFIPNIYLAVWTTTPWTMPANAAVAVNAKLQYSVVEVQSFSEDES 372

Query: 133 LVV--KKKLLG-------------------------------------SELENCSYIHPN 161
            V   KKK+ G                                     S+LENC Y HP 
Sbjct: 373 TVTSNKKKIPGKVLKNQQKLFVIVATDLVPALEAKWGVKLSISKTFLGSDLENCRYTHPI 432

[Close]
BLAST of Cucsa.253070 vs. NCBI nr
Match: gi|778678894|ref|XP_011651056.1| (PREDICTED: LOW QUALITY PROTEIN: probable isoleucine--tRNA ligase, mitochondrial [Cucumis sativus])

HSP 1 Score: 218.8 bits (556), Expect = 7.2e-54
Identity = 122/193 (63.21%), Postives = 127/193 (65.80%), Query Frame = 1 

Query: 13  LTLSPEYEAVQIEVFGQMAIQGYIFRGRKPVHWSPSSRTALAEAELEYPEGHTSRSIYAI 72
           LTLSPEYEA QIEVFGQMAIQGYIFRGRKPVHWSPSSRTALAEAELEYPEGHTSRSIYAI
Sbjct: 259 LTLSPEYEAAQIEVFGQMAIQGYIFRGRKPVHWSPSSRTALAEAELEYPEGHTSRSIYAI 318

Query: 73  FRLVKEGPSSGGVLQDFFRDLCLAIWTTTPWTIPANAG------LQQILCQLLEAKWGVK 132
           FRLVKEGPSSGGVLQ+F  DLCLAIWTTTPWTIPANA       L   + +LL     V 
Sbjct: 319 FRLVKEGPSSGGVLQEFLPDLCLAIWTTTPWTIPANAAVAVNAKLHYAIIELLAPSEDVS 378

Query: 133 ---------------------LVVKKKLL------------------GSELENCSYIHPN 161
                                LVV   L+                  GSELENCSYIHP 
Sbjct: 379 VLTGNKKKRPGNILKEGKRPFLVVASDLVPTLEAKWGVKLVVKKKLLGSELENCSYIHPI 438

[Close]
BLAST of Cucsa.253070 vs. NCBI nr
Match: gi|659076823|ref|XP_008438885.1| (PREDICTED: isoleucine--tRNA ligase, mitochondrial [Cucumis melo])

HSP 1 Score: 218.8 bits (556), Expect = 7.2e-54
Identity = 124/193 (64.25%), Postives = 129/193 (66.84%), Query Frame = 1 

Query: 13  LTLSPEYEAVQIEVFGQMAIQGYIFRGRKPVHWSPSSRTALAEAELEYPEGHTSRSIYAI 72
           LTLSPEYEA QIEVFGQMAIQGYIFRGRKPVHWSPSSRTALAEAELEYPEGHTSRSIYAI
Sbjct: 259 LTLSPEYEAAQIEVFGQMAIQGYIFRGRKPVHWSPSSRTALAEAELEYPEGHTSRSIYAI 318

Query: 73  FRLVKEGPSSGGVLQDFFRDLCLAIWTTTPWTIPANAG------LQQILCQLLE------ 132
           FRLVKEGPSSGGVLQ+F  DLCLAIWTTTPWTIPANA       L   + +LL       
Sbjct: 319 FRLVKEGPSSGGVLQEFLPDLCLAIWTTTPWTIPANAAVAVNAKLHYAIIELLAPSEDAS 378

Query: 133 -------------AKWGVK--LVVKKKLL------------------GSELENCSYIHPN 161
                         K G +  LVV   L+                  GSELENCSYIHP 
Sbjct: 379 VLTGNKKKKPGNILKEGKRPFLVVASDLVPTLEAKWGVKIVVKKKLLGSELENCSYIHPI 438

[Close]
BLAST of Cucsa.253070 vs. NCBI nr
Match: gi|223544009|gb|EEF45535.1| (isoleucyl tRNA synthetase, putative [Ricinus communis])

HSP 1 Score: 206.1 bits (523), Expect = 4.8e-50
Identity = 109/192 (56.77%), Postives = 124/192 (64.58%), Query Frame = 1 

Query: 13  LTLSPEYEAVQIEVFGQMAIQGYIFRGRKPVHWSPSSRTALAEAELEYPEGHTSRSIYAI 72
           LTL P+YEA QIEVFGQMA+QGYI+RGRKPVHWSPSS TALAEAELEYPEGH S+SIYAI
Sbjct: 264 LTLDPDYEAAQIEVFGQMALQGYIYRGRKPVHWSPSSHTALAEAELEYPEGHVSKSIYAI 323

Query: 73  FRLVKEGPSSGGVLQDFFRDLCLAIWTTTPWTIPANAG------LQQILCQL-------- 132
           FR+    P+S  +L++FF DL LAIWTTTPWT+PANA       LQ  + ++        
Sbjct: 324 FRVASAPPTSRLLLEEFFPDLFLAIWTTTPWTVPANAAVAVNSKLQYAVVEVQSLEDAST 383

Query: 133 ------------------------------LEAKWGVKLVVKKKLLGSELENCSYIHPNG 161
                                         LEAKW VKLV+KK L GS+LENC YIHP  
Sbjct: 384 SPGNKKRRFGNVLREQKKLFLIVASDLMPTLEAKWSVKLVIKKTLSGSDLENCRYIHPID 443

[Close]
BLAST of Cucsa.253070 vs. NCBI nr
Match: gi|1000971596|ref|XP_015573355.1| (PREDICTED: isoleucine--tRNA ligase, chloroplastic/mitochondrial [Ricinus communis])

HSP 1 Score: 206.1 bits (523), Expect = 4.8e-50
Identity = 109/192 (56.77%), Postives = 124/192 (64.58%), Query Frame = 1 

Query: 13  LTLSPEYEAVQIEVFGQMAIQGYIFRGRKPVHWSPSSRTALAEAELEYPEGHTSRSIYAI 72
           LTL P+YEA QIEVFGQMA+QGYI+RGRKPVHWSPSS TALAEAELEYPEGH S+SIYAI
Sbjct: 254 LTLDPDYEAAQIEVFGQMALQGYIYRGRKPVHWSPSSHTALAEAELEYPEGHVSKSIYAI 313

Query: 73  FRLVKEGPSSGGVLQDFFRDLCLAIWTTTPWTIPANAG------LQQILCQL-------- 132
           FR+    P+S  +L++FF DL LAIWTTTPWT+PANA       LQ  + ++        
Sbjct: 314 FRVASAPPTSRLLLEEFFPDLFLAIWTTTPWTVPANAAVAVNSKLQYAVVEVQSLEDAST 373

Query: 133 ------------------------------LEAKWGVKLVVKKKLLGSELENCSYIHPNG 161
                                         LEAKW VKLV+KK L GS+LENC YIHP  
Sbjct: 374 SPGNKKRRFGNVLREQKKLFLIVASDLMPTLEAKWSVKLVIKKTLSGSDLENCRYIHPID 433

[Close]
BLAST of Cucsa.253070 vs. NCBI nr
Match: gi|661897159|emb|CDO98871.1| (unnamed protein product [Coffea canephora])

HSP 1 Score: 204.5 bits (519), Expect = 1.4e-49
Identity = 109/193 (56.48%), Postives = 126/193 (65.28%), Query Frame = 1 

Query: 13  LTLSPEYEAVQIEVFGQMAIQGYIFRGRKPVHWSPSSRTALAEAELEYPEGHTSRSIYAI 72
           LTL PEYEA QIEVFG+MAIQG+I+RGRKPVHWSPSSRTALAEAELEYPEGH S S+YAI
Sbjct: 93  LTLDPEYEAAQIEVFGKMAIQGFIYRGRKPVHWSPSSRTALAEAELEYPEGHVSNSMYAI 152

Query: 73  FRLVKEGPSSGGVLQDFFRDLCLAIWTTTPWTIPANAG------LQQILCQLL------- 132
           F+LV   P+   +L++FF +LCLAIWTTTPWTIPANA       LQ  + ++        
Sbjct: 153 FKLV-NAPNLCNLLEEFFPNLCLAIWTTTPWTIPANAAVAVNPKLQYAIVEVQSGSTDSS 212

Query: 133 --------------------------------EAKWGVKLVVKKKLLGSELENCSYIHPN 161
                                           EAKWG KL++KKKLLGS+LENCSY+HP 
Sbjct: 213 TLSGNAKKKLGNVFEEYEKLFFIVALDLVPTLEAKWGKKLLIKKKLLGSDLENCSYVHPI 272

The following BLAST results are available for this feature:
BLAST of Cucsa.253070 vs. Swiss-Prot
Match NameE-valueIdentityDescription
SYI_SYNY31.8e-4152.02Isoleucine--tRNA ligase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) GN=ileS ... [more]
SYIM_ARATH4.1e-4150.26Isoleucine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana GN=... [more]
SYI_CYAP77.8e-4049.13Isoleucine--tRNA ligase OS=Cyanothece sp. (strain PCC 7424) GN=ileS PE=3 SV=1[more]
SYI_SYNP66.1e-3752.38Isoleucine--tRNA ligase OS=Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAU... [more]
SYI_NOSP71.4e-3646.86Isoleucine--tRNA ligase OS=Nostoc punctiforme (strain ATCC 29133 / PCC 73102) GN... [more]
BLAST of Cucsa.253070 vs. TrEMBL
Match NameE-valueIdentityDescription
M0TSR7_MUSAM2.5e-5364.24Uncharacterized protein OS=Musa acuminata subsp. malaccensis PE=3 SV=1[more]
B9RT52_RICCO3.4e-5056.77Isoleucyl tRNA synthetase, putative OS=Ricinus communis GN=RCOM_0681070 PE=3 SV=... [more]
A0A068TRK4_COFCA9.8e-5056.48Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00025819001 PE=4 SV=1[more]
A5ALP9_VITVI5.1e-4656.48Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_015862 PE=3 SV=1[more]
F6HDF8_VITVI5.1e-4656.48Putative uncharacterized protein OS=Vitis vinifera GN=VIT_05s0020g01550 PE=3 SV=... [more]
BLAST of Cucsa.253070 vs. TAIR10
Match NameE-valueIdentityDescription
AT5G49030.12.3e-4250.26 tRNA synthetase class I (I, L, M and V) family protein[more]
BLAST of Cucsa.253070 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|778678894|ref|XP_011651056.1|7.2e-5463.21PREDICTED: LOW QUALITY PROTEIN: probable isoleucine--tRNA ligase, mitochondrial ... [more]
gi|659076823|ref|XP_008438885.1|7.2e-5464.25PREDICTED: isoleucine--tRNA ligase, mitochondrial [Cucumis melo][more]
gi|223544009|gb|EEF45535.1|4.8e-5056.77isoleucyl tRNA synthetase, putative [Ricinus communis][more]
gi|1000971596|ref|XP_015573355.1|4.8e-5056.77PREDICTED: isoleucine--tRNA ligase, chloroplastic/mitochondrial [Ricinus communi... [more]
gi|661897159|emb|CDO98871.1|1.4e-4956.48unnamed protein product [Coffea canephora][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR002300aa-tRNA-synth_Ia
IPR009008Val/Leu/Ile-tRNA-synth_edit
IPR014729Rossmann-like_a/b/a_fold
Vocabulary: Molecular Function
TermDefinition
GO:0000166nucleotide binding
GO:0004812aminoacyl-tRNA ligase activity
GO:0005524ATP binding
GO:0002161aminoacyl-tRNA editing activity
Vocabulary: Biological Process
TermDefinition
GO:0006418tRNA aminoacylation for protein translation
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006412 translation
biological_process GO:0006418 tRNA aminoacylation for protein translation
cellular_component GO:0005737 cytoplasm
molecular_function GO:0002161 aminoacyl-tRNA editing activity
molecular_function GO:0004812 aminoacyl-tRNA ligase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0000166 nucleotide binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.253070.1Cucsa.253070.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002300Aminoacyl-tRNA synthetase, class IaPFAMPF00133tRNA-synt_1coord: 12..112
score: 6.9
IPR009008Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domainGENE3DG3DSA:3.90.740.10coord: 55..109
score: 1.4
IPR009008Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domainunknownSSF50677ValRS/IleRS/LeuRS editing domaincoord: 65..157
score: 1.
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3DG3DSA:3.40.50.620coord: 12..54
score: 1.1
NoneNo IPR availablePANTHERPTHR11946ISOLEUCYL, LEUCYL, TYROSYL, VALYL AND METHIONYL-TRNA SYNTHETASEScoord: 13..160
score: 9.3
NoneNo IPR availablePANTHERPTHR11946:SF82ISOLEUCINE--TRNA LIGASE, MITOCHONDRIALcoord: 13..160
score: 9.3
NoneNo IPR availableunknownSSF52374Nucleotidylyl transferasecoord: 12..110
score: 3.15

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cucsa.253070Cla021742Watermelon (97103) v1cgywmB479
Cucsa.253070MELO3C006665Melon (DHL92) v3.5.1cgymeB439
Cucsa.253070ClCG05G006110Watermelon (Charleston Gray)cgywcgB451
Cucsa.253070CSPI03G14420Wild cucumber (PI 183967)cgycpiB383
Cucsa.253070CmaCh14G016190Cucurbita maxima (Rimu)cgycmaB0709
Cucsa.253070CmoCh14G016560Cucurbita moschata (Rifu)cgycmoB0700
Cucsa.253070Lsi05G015400Bottle gourd (USVL1VR-Ls)cgylsiB420
Cucsa.253070Cp4.1LG03g11460Cucurbita pepo (Zucchini)cgycpeB0682
Cucsa.253070MELO3C006665.2Melon (DHL92) v3.6.1cgymedB432
Cucsa.253070CsaV3_3G014640Cucumber (Chinese Long) v3cgycucB387
Cucsa.253070Cla97C05G087630Watermelon (97103) v2cgywmbB452
Cucsa.253070Bhi01G000720Wax gourdcgywgoB522
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cucsa.253070Melon (DHL92) v3.6.1cgymedB425
Cucsa.253070Melon (DHL92) v3.6.1cgymedB427
Cucsa.253070Silver-seed gourdcarcgyB0083
Cucsa.253070Silver-seed gourdcarcgyB0133
Cucsa.253070Silver-seed gourdcarcgyB0681
Cucsa.253070Silver-seed gourdcarcgyB0788
Cucsa.253070Silver-seed gourdcarcgyB0960
Cucsa.253070Cucumber (Chinese Long) v3cgycucB382
Cucsa.253070Watermelon (97103) v2cgywmbB453
Cucsa.253070Wax gourdcgywgoB505
Cucsa.253070Cucurbita maxima (Rimu)cgycmaB0703
Cucsa.253070Cucurbita maxima (Rimu)cgycmaB0713
Cucsa.253070Cucurbita maxima (Rimu)cgycmaB0714
Cucsa.253070Cucurbita maxima (Rimu)cgycmaB0727
Cucsa.253070Cucurbita maxima (Rimu)cgycmaB0731
Cucsa.253070Cucurbita moschata (Rifu)cgycmoB0707
Cucsa.253070Cucurbita moschata (Rifu)cgycmoB0718
Cucsa.253070Cucurbita moschata (Rifu)cgycmoB0720
Cucsa.253070Wild cucumber (PI 183967)cgycpiB376
Cucsa.253070Cucumber (Chinese Long) v2cgycuB360
Cucsa.253070Cucumber (Chinese Long) v2cgycuB366
Cucsa.253070Melon (DHL92) v3.5.1cgymeB430
Cucsa.253070Watermelon (Charleston Gray)cgywcgB453
Cucsa.253070Watermelon (97103) v1cgywmB483
Cucsa.253070Cucurbita pepo (Zucchini)cgycpeB0669
Cucsa.253070Cucurbita pepo (Zucchini)cgycpeB0671
Cucsa.253070Cucurbita pepo (Zucchini)cgycpeB0678
Cucsa.253070Cucurbita pepo (Zucchini)cgycpeB0687
Cucsa.253070Bottle gourd (USVL1VR-Ls)cgylsiB412