Cucsa.251580 (gene) Cucumber (Gy14) v1

Overview
NameCucsa.251580
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionPlant invertase/pectin methylesterase inhibitor superfamily protein
Locationscaffold02229 : 621219 .. 621803 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTATCTCAAACTCCAAGAAAAGTTTGATTCTTTTCCAATTAATTCTCCAATTAACACTGATTCATTCAGCCATAACTCCACAAAGCAGCACAGAGTTCATAAAAAGCTCTTGCAGTTCCACCACTTACCCTCGTCTCTGTTTTTCATCTCTTTCAGTCCATGCCAATGCCATCCAAACAAGTCCCCGCCTTTTGGCCACTGCCGCCCTCTCCGTTTCCCTCTCCTCCGTCAAGTCCACCGCCACTCAAATCCTCAAGCTTTCTCACTCCCATGGCCTCCCCTCCAGAGATGTCTCTGCTCTTGACGACTGTTTGGAAGAACTGAGTGACTCTGTCGACTCCCTCGCTGCTTCCATTTCCGAGATGCCGAAGCTCCGTGGAACCAATTTCGACCTCGCAATGAGTAATGTTCAGACATGGGTTAGCGCCGCTCTGACGGATGAAACCACATGTAGCGAAGGGTTTCAGGGGAAGACGGTGAACGGAGGCGTTAAGGGTGTGGTTAGGACTAAGATTGTCAACATTGCGCAATTGACTAGTAACGCTCTGTCTTTGATTAATCAAATTGGTGATCTGCATTAG

mRNA sequence

ATGGGTATCTCAAACTCCAAGAAAAGTTTGATTCTTTTCCAATTAATTCTCCAATTAACACTGATTCATTCAGCCATAACTCCACAAAGCAGCACAGAGTTCATAAAAAGCTCTTGCAGTTCCACCACTTACCCTCGTCTCTGTTTTTCATCTCTTTCAGTCCATGCCAATGCCATCCAAACAAGTCCCCGCCTTTTGGCCACTGCCGCCCTCTCCGTTTCCCTCTCCTCCGTCAAGTCCACCGCCACTCAAATCCTCAAGCTTTCTCACTCCCATGGCCTCCCCTCCAGAGATGTCTCTGCTCTTGACGACTGTTTGGAAGAACTGAGTGACTCTGTCGACTCCCTCGCTGCTTCCATTTCCGAGATGCCGAAGCTCCGTGGAACCAATTTCGACCTCGCAATGAGTAATGTTCAGACATGGGTTAGCGCCGCTCTGACGGATGAAACCACATGTAGCGAAGGGTTTCAGGGGAAGACGGTGAACGGAGGCGTTAAGGGTGTGGTTAGGACTAAGATTGTCAACATTGCGCAATTGACTAGTAACGCTCTGTCTTTGATTAATCAAATTGGTGATCTGCATTAG

Coding sequence (CDS)

ATGGGTATCTCAAACTCCAAGAAAAGTTTGATTCTTTTCCAATTAATTCTCCAATTAACACTGATTCATTCAGCCATAACTCCACAAAGCAGCACAGAGTTCATAAAAAGCTCTTGCAGTTCCACCACTTACCCTCGTCTCTGTTTTTCATCTCTTTCAGTCCATGCCAATGCCATCCAAACAAGTCCCCGCCTTTTGGCCACTGCCGCCCTCTCCGTTTCCCTCTCCTCCGTCAAGTCCACCGCCACTCAAATCCTCAAGCTTTCTCACTCCCATGGCCTCCCCTCCAGAGATGTCTCTGCTCTTGACGACTGTTTGGAAGAACTGAGTGACTCTGTCGACTCCCTCGCTGCTTCCATTTCCGAGATGCCGAAGCTCCGTGGAACCAATTTCGACCTCGCAATGAGTAATGTTCAGACATGGGTTAGCGCCGCTCTGACGGATGAAACCACATGTAGCGAAGGGTTTCAGGGGAAGACGGTGAACGGAGGCGTTAAGGGTGTGGTTAGGACTAAGATTGTCAACATTGCGCAATTGACTAGTAACGCTCTGTCTTTGATTAATCAAATTGGTGATCTGCATTAG

Protein sequence

MGISNSKKSLILFQLILQLTLIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQTSPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASISEMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKGVVRTKIVNIAQLTSNALSLINQIGDLH*
Homology
[Close]
BLAST of Cucsa.251580 vs. Swiss-Prot
Match: 21KD_DAUCA (21 kDa protein OS=Daucus carota PE=2 SV=1)

HSP 1 Score: 162.2 bits (409), Expect = 5.9e-39
Identity = 85/190 (44.74%), Postives = 126/190 (66.32%), Query Frame = 1 

Query: 8   KSLILFQLILQLTLIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQTSPRLLA 67
           KS ++F  +L   +I +A +   + +FIK+SC+ TTYP +C  SLS +A  IQ +P+ LA
Sbjct: 5   KSTLVFSALL---VILAAASAAPANQFIKTSCTLTTYPAVCEQSLSAYAKTIQNNPQELA 64

Query: 68  TAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASISEMPKL- 127
           + AL VSL+  +   T + +L+   GL +R  +A+ DCLEE+ DS+D ++ S  EM  L 
Sbjct: 65  STALQVSLTRTQQAQTFMKRLNKFKGLKARQYAAIHDCLEEVEDSLDRVSRSCDEMKNLS 124

Query: 128 --RGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKGVVRTKIVNIAQLTSNAL 187
             +G +F   MSNV+TWVSAALTDETTC +GF GK ++G +K  VR ++V +A++TSNAL
Sbjct: 125 HAKGNDFTFRMSNVETWVSAALTDETTCMDGFAGKGMDGKIKESVRAQVVAVARVTSNAL 184

Query: 188 SLINQIGDLH 195
           +L+N     H
Sbjct: 185 ALVNNFAAKH 191

[Close]
BLAST of Cucsa.251580 vs. Swiss-Prot
Match: PME2_ARATH (Pectinesterase 2 OS=Arabidopsis thaliana GN=PME2 PE=2 SV=2)

HSP 1 Score: 85.9 bits (211), Expect = 5.4e-16
Identity = 52/169 (30.77%), Postives = 96/169 (56.80%), Query Frame = 1 

Query: 26  ITPQSSTE--FIKSSCSSTTYPRLCFSSLSVHANAIQTSPRLLATAALSVSLSSVKSTAT 85
           IT  SST    +KS CSST YP LCFS+++       TS + +  A+L+++  +VK    
Sbjct: 51  ITTLSSTSHAILKSVCSSTLYPELCFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYF 110

Query: 86  QILKL-SHSHGLPSRDVSALDDCLEELSDSVDSLAASISEMPKL-RGTNFDLAMSNVQTW 145
            + KL +   GL  R+V+AL DCLE + +++D L  ++ ++ +  +  +      +++T 
Sbjct: 111 AVKKLIAKRKGLTPREVTALHDCLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTL 170

Query: 146 VSAALTDETTCSEGFQGKTVNGGVKGVVRTKIVNIAQLTSNALSLINQI 191
           +S+A+T++ TC +GF     +  V+  +    V++  + SNAL++I  +
Sbjct: 171 ISSAITNQGTCLDGFSYDDADRKVRKALLKGQVHVEHMCSNALAMIKNM 219

[Close]
BLAST of Cucsa.251580 vs. Swiss-Prot
Match: PME1_CITSI (Pectinesterase 1 OS=Citrus sinensis GN=PECS-1.1 PE=2 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 2.7e-15
Identity = 49/161 (30.43%), Postives = 91/161 (56.52%), Query Frame = 1 

Query: 35  IKSSCSSTTYPRLCFSSLSVHANAIQ--TSPRLLATAALSVSLSSVKSTATQILKLSHSH 94
           +KSSCSST YP LCFS+++    A +  TS + +   +L+++ ++V+     I KL    
Sbjct: 69  LKSSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRT 128

Query: 95  GLPSRDVSALDDCLEELSDSVDSLAASISEMPKLRGT-NFDLAMSNVQTWVSAALTDETT 154
            L  R+  AL DCLE + +++D L  ++ ++ +     +      +++T +SAA+T++ T
Sbjct: 129 NLTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGT 188

Query: 155 CSEGFQGKTVNGGVKGVVRTKIVNIAQLTSNALSLINQIGD 193
           C +GF     N  V+  +    V++ ++ SNAL++I  + D
Sbjct: 189 CLDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTD 229

[Close]
BLAST of Cucsa.251580 vs. Swiss-Prot
Match: PME3_CITSI (Pectinesterase 3 OS=Citrus sinensis PE=1 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 2.7e-15
Identity = 49/161 (30.43%), Postives = 91/161 (56.52%), Query Frame = 1 

Query: 35  IKSSCSSTTYPRLCFSSLSVHANAIQ--TSPRLLATAALSVSLSSVKSTATQILKLSHSH 94
           +KSSCSST YP LCFS+++    A +  TS + +   +L+++ ++V+     I KL    
Sbjct: 69  LKSSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRT 128

Query: 95  GLPSRDVSALDDCLEELSDSVDSLAASISEMPKLRGT-NFDLAMSNVQTWVSAALTDETT 154
            L  R+  AL DCLE + +++D L  ++ ++ +     +      +++T +SAA+T++ T
Sbjct: 129 NLTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGT 188

Query: 155 CSEGFQGKTVNGGVKGVVRTKIVNIAQLTSNALSLINQIGD 193
           C +GF     N  V+  +    V++ ++ SNAL++I  + D
Sbjct: 189 CLDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTD 229

[Close]
BLAST of Cucsa.251580 vs. Swiss-Prot
Match: PME34_ARATH (Probable pectinesterase/pectinesterase inhibitor 34 OS=Arabidopsis thaliana GN=PME34 PE=2 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 1.3e-14
Identity = 59/205 (28.78%), Postives = 100/205 (48.78%), Query Frame = 1 

Query: 6   SKKSLILFQLILQLTLIHSAIT-----------------PQSSTEFIKSSCSSTTYPRLC 65
           SKK L++  ++L ++LI +A                    +  ++ I  +C  T +P LC
Sbjct: 41  SKKKLVVSSIVLAISLILAAAIFAGVRSRLKLNQSVPGLARKPSQAISKACELTRFPELC 100

Query: 66  FSSLSVHANAIQ-TSPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLE 125
             SL     ++  +S + L    ++++L            LS    +P R  SA D C+E
Sbjct: 101 VDSLMDFPGSLAASSSKDLIHVTVNMTLHHFSHALYSSASLSFVD-MPPRARSAYDSCVE 160

Query: 126 ELSDSVDSLAASISEMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKG 185
            L DSVD+L+ ++S +      +      +V TW+SAALT+  TC+EGF G   +GGVK 
Sbjct: 161 LLDDSVDALSRALSSV-----VSSSAKPQDVTTWLSAALTNHDTCTEGFDG-VDDGGVKD 220

Query: 186 VVRTKIVNIAQLTSNALSLINQIGD 193
            +   + N+++L SN L++ +   D
Sbjct: 221 HMTAALQNLSELVSNCLAIFSASHD 238

[Close]
BLAST of Cucsa.251580 vs. TrEMBL
Match: A0A0A0L8D1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G175740 PE=4 SV=1)

HSP 1 Score: 365.5 bits (937), Expect = 3.9e-98
Identity = 191/193 (98.96%), Postives = 192/193 (99.48%), Query Frame = 1 

Query: 2   GISNSKKSLILFQLILQLTLIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQT 61
           GISNSKKSLILFQLILQLTLIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQT
Sbjct: 44  GISNSKKSLILFQLILQLTLIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQT 103

Query: 62  SPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASIS 121
           SPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASIS
Sbjct: 104 SPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASIS 163

Query: 122 EMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKGVVRTKIVNIAQLTS 181
           EMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKG VRTKIVNIAQLTS
Sbjct: 164 EMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKGAVRTKIVNIAQLTS 223

Query: 182 NALSLINQIGDLH 195
           NALSLIN+IGDLH
Sbjct: 224 NALSLINRIGDLH 236

[Close]
BLAST of Cucsa.251580 vs. TrEMBL
Match: A0A0A0LB27_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G176245 PE=4 SV=1)

HSP 1 Score: 364.0 bits (933), Expect = 1.1e-97
Identity = 190/194 (97.94%), Postives = 192/194 (98.97%), Query Frame = 1 

Query: 1   MGISNSKKSLILFQLILQLTLIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ 60
           MGISNSKK+LILFQLILQLTLIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ
Sbjct: 1   MGISNSKKTLILFQLILQLTLIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ 60

Query: 61  TSPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASI 120
           TSPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASI
Sbjct: 61  TSPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASI 120

Query: 121 SEMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKGVVRTKIVNIAQLT 180
           SEMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKG VRTKIVNIAQLT
Sbjct: 121 SEMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKGAVRTKIVNIAQLT 180

Query: 181 SNALSLINQIGDLH 195
           SNALSLIN+I DLH
Sbjct: 181 SNALSLINRIADLH 194

[Close]
BLAST of Cucsa.251580 vs. TrEMBL
Match: A0A061G793_THECC (DC1.2-like, putative OS=Theobroma cacao GN=TCM_014946 PE=4 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 2.0e-54
Identity = 114/194 (58.76%), Postives = 146/194 (75.26%), Query Frame = 1 

Query: 4   SNSKKSLILFQLILQLTLIHSA---ITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ 63
           S+S K L++   I       SA   I PQSS EFI++SCSSTTYP+LCF +LS HA+ IQ
Sbjct: 7   SHSFKFLLILLAINSYINFSSAGRQIAPQSSAEFIRTSCSSTTYPKLCFETLSTHASLIQ 66

Query: 64  TSPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASI 123
           TSP+LLA AAL+V+LS+ +ST+  ++ LS SHGL  R+  A+ DC+EELSDS+D L  SI
Sbjct: 67  TSPQLLAHAALNVTLSTTESTSAMMVTLSKSHGLKPREAEAMQDCVEELSDSIDELRKSI 126

Query: 124 SEMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKGVVRTKIVNIAQLT 183
           SEM +++G+NF L +++VQTWVSAALTDE+TCS+GFQG  +NG VK  VRTKI+ IA LT
Sbjct: 127 SEMGQVKGSNFGLMINDVQTWVSAALTDESTCSDGFQGNNMNGNVKTAVRTKILRIAHLT 186

Query: 184 SNALSLINQIGDLH 195
           SNAL+LIN    LH
Sbjct: 187 SNALALINNYASLH 200

[Close]
BLAST of Cucsa.251580 vs. TrEMBL
Match: M5XKR0_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011607mg PE=4 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 1.5e-52
Identity = 108/179 (60.34%), Postives = 139/179 (77.65%), Query Frame = 1 

Query: 16  ILQLTLIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQTSPRLLATAALSVSL 75
           I+  T     + P+++TEFIK+SCS+TTYP+LC +SLS HA+ IQTSP+L+A AAL+V+L
Sbjct: 24  IISSTSAARDLAPKTNTEFIKTSCSATTYPKLCLTSLSSHASTIQTSPKLMAGAALNVTL 83

Query: 76  SSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASISEMPKLRGTNFDLAM 135
           SS KST+  +LKLS SHGL  ++V A+ DCLEELSDSVD L +SI+EM   +  +F L +
Sbjct: 84  SSAKSTSAVMLKLSQSHGLKPKEVGAMRDCLEELSDSVDELQSSIAEMGNFKSYDFQLMI 143

Query: 136 SNVQTWVSAALTDETTCSEGFQGKTVNGGVKGVVRTKIVNIAQLTSNALSLINQIGDLH 195
           S+VQTWVSAALTDE TCSEGF G  +NG +K  VR +IVNIAQLTSNAL+LIN+   +H
Sbjct: 144 SDVQTWVSAALTDENTCSEGFGGNGMNGNLKTAVRGRIVNIAQLTSNALALINRYASVH 202

[Close]
BLAST of Cucsa.251580 vs. TrEMBL
Match: B9R9R0_RICCO (21 kDa protein, putative OS=Ricinus communis GN=RCOM_1500140 PE=4 SV=1)

HSP 1 Score: 210.7 bits (535), Expect = 1.6e-51
Identity = 106/195 (54.36%), Postives = 145/195 (74.36%), Query Frame = 1 

Query: 4   SNSKKSLILFQLILQLTLIHSA----ITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAI 63
           SN+  + +L  L +   +  S+    +   +STEFI++SCS+TTYPRLC+SSL +HA+ I
Sbjct: 6   SNTFSNFLLILLAISFHINSSSAGRNLAQTTSTEFIRTSCSTTTYPRLCYSSLKIHASKI 65

Query: 64  QTSPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAAS 123
           QTSP LLA AAL+V+L+S +ST+T + KLS SHG+  R+VSA+ DC++EL+DSVD L  S
Sbjct: 66  QTSPMLLANAALNVTLASTRSTSTMMQKLSKSHGMKPREVSAMQDCMDELTDSVDELRKS 125

Query: 124 ISEMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKGVVRTKIVNIAQL 183
           I E+ K +G+ F L M++VQTWVSAALTDE+TCS+GF G T+NG +K +VR + V IA L
Sbjct: 126 IDELGKAQGSKFGLMMNDVQTWVSAALTDESTCSDGFAGSTMNGELKTLVRQQTVKIAHL 185

Query: 184 TSNALSLINQIGDLH 195
           TSNALSL+N    +H
Sbjct: 186 TSNALSLVNSYASVH 200

[Close]
BLAST of Cucsa.251580 vs. TAIR10
Match: AT5G62360.1 (AT5G62360.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 189.5 bits (480), Expect = 1.9e-48
Identity = 95/184 (51.63%), Postives = 140/184 (76.09%), Query Frame = 1 

Query: 15  LILQLTLIHSAITPQSST----EFIKSSCSSTTYPRLCFSSLSVHANAIQTSPRLLATAA 74
           +I  L L+H+  T  ++T    EF+KSSC+ TTYPRLCFSSLS HA+ IQTSP+L+A AA
Sbjct: 20  IIAMLKLVHTTTTTTTTTTTNTEFVKSSCTFTTYPRLCFSSLSTHASLIQTSPKLMAHAA 79

Query: 75  LSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASISEMPKLRGTN 134
           L+++L+S K T+  +++LS+S  L  ++VSA+ DC+EEL D+++ L  SI EM +L G+N
Sbjct: 80  LNITLASAKVTSAMMVRLSNSR-LKPKEVSAMRDCVEELGDTLEELRKSIGEMCQLSGSN 139

Query: 135 FDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKGVVRTKIVNIAQLTSNALSLINQI 194
           +++ +S++QTWVSAALTD  TC++GF+G+ ++G VK +VR +I+ IA LTSNAL+LIN  
Sbjct: 140 YEVYISDIQTWVSAALTDVNTCTDGFEGEDMDGKVKVLVRGRILVIAHLTSNALALINHF 199

[Close]
BLAST of Cucsa.251580 vs. TAIR10
Match: AT5G62350.1 (AT5G62350.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 163.7 bits (413), Expect = 1.1e-40
Identity = 91/186 (48.92%), Postives = 124/186 (66.67%), Query Frame = 1 

Query: 11  ILFQLILQLTLIHSAITPQSSTE---FIKSSCSSTTYPRLCFSSLSVHANAIQTSPRLLA 70
           I + L L+LT   +A    +S +   FI+SSC +TTYP LC  SLSV+AN IQTSP+ LA
Sbjct: 12  ISYLLSLELTAATAASQTGASKKAINFIQSSCKTTTYPALCVHSLSVYANDIQTSPKRLA 71

Query: 71  TAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASISEMPKLR 130
             A++V+LS  +ST   + +L+   GL  R+V A+ DC+EE++D+VD L  S+ E+ KL 
Sbjct: 72  ETAIAVTLSRAQSTKLFVSRLTRMKGLKKREVEAIKDCVEEMNDTVDRLTKSVQEL-KLC 131

Query: 131 GT-----NFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKGVVRTKIVNIAQLTSN 189
           G+      F   MSN QTW SAALTDE TCS+GF G+ ++G +K  VR +I+N+   TSN
Sbjct: 132 GSAKDQDQFAYHMSNAQTWTSAALTDENTCSDGFSGRVMDGRIKNSVRARIMNVGHETSN 191

[Close]
BLAST of Cucsa.251580 vs. TAIR10
Match: AT4G25260.1 (AT4G25260.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 161.8 bits (408), Expect = 4.3e-40
Identity = 91/198 (45.96%), Postives = 126/198 (63.64%), Query Frame = 1 

Query: 5   NSKKSLILFQLILQLTLIHSA--ITPQSS--TEFIKSSCSSTTYPRLCFSSLSVHANAIQ 64
           N + SLILF L L    I  A  +  +SS  TEFIK+SC +T+YP  CF SLS +A+ I+
Sbjct: 4   NFELSLILFVLYLSTAAIVMARNLEEESSGDTEFIKASCETTSYPDRCFQSLSSYASEIK 63

Query: 65  TSPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASI 124
             PR LA  AL+VS++  KS  T + +++   G+  R   A+ DCLEE+ D+VD L+ S+
Sbjct: 64  KQPRKLAETALAVSIARAKSAKTYVSEMTDYKGITKRQHEAVADCLEEMGDTVDRLSNSL 123

Query: 125 SEMPKLR----GTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKGVVRTKIVNI 184
            E+  L     G +F   +SNV+TW SAALTDET C +GF GK + G +K ++RT IV++
Sbjct: 124 KELKHLEEGDSGEDFWFCLSNVRTWTSAALTDETACMDGFGGKAMAGELKSLIRTHIVSV 183

Query: 185 AQLTSNALSLINQIGDLH 195
           A+ TSNAL+LIN     H
Sbjct: 184 AEETSNALALINDFASKH 201

[Close]
BLAST of Cucsa.251580 vs. TAIR10
Match: AT3G47380.1 (AT3G47380.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 150.6 bits (379), Expect = 1.0e-36
Identity = 79/170 (46.47%), Postives = 116/170 (68.24%), Query Frame = 1 

Query: 24  SAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQTSPRLLATAALSVSLSSVKSTAT 83
           +AIT + +  FI++SC +TTYP +C +SL+ +AN+IQTSPR LA  AL+V+++  +ST  
Sbjct: 28  AAITSKRAINFIQASCKATTYPTVCVNSLTGYANSIQTSPRRLAETALNVTVTQAQSTKV 87

Query: 84  QILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASISEMPKLRGT-----NFDLAMSNV 143
            + +L     L  R++ A+ DC+EE+ D+VD L  SI E+ K+ G+      F   MSN 
Sbjct: 88  FVWRLGRFTSLKKREIQAVKDCIEEIHDAVDRLTMSIHEV-KMCGSAKGRDQFWFHMSNA 147

Query: 144 QTWVSAALTDETTCSEGFQGKTVNGGVKGVVRTKIVNIAQLTSNALSLIN 189
           QTW SAALT+  TCS+GF G+ ++G VK  VR +I+N+ + TSNAL+LIN
Sbjct: 148 QTWTSAALTNANTCSDGFAGRVMDGRVKNSVRARILNLGRGTSNALALIN 196

[Close]
BLAST of Cucsa.251580 vs. TAIR10
Match: AT1G62760.1 (AT1G62760.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 141.4 bits (355), Expect = 6.1e-34
Identity = 74/168 (44.05%), Postives = 114/168 (67.86%), Query Frame = 1 

Query: 24  SAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQTSPRLLATAALSVSLSSVKSTAT 83
           S  + Q++ ++IK+SC+ T Y  +C++SLS +A+ I+++P+ LA  AL+++LSS KS + 
Sbjct: 137 STYSNQTNLDYIKTSCNITLYKTICYNSLSPYASTIRSNPQKLAVIALNLTLSSAKSASK 196

Query: 84  QILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASISEMPKLR---GTNFDLAMSNVQT 143
            +  +SH  GL   +V A+ DC+EE+ DSV SL  SI E+  +       F++ MS+V+T
Sbjct: 197 FVKNISHGGGLTRLEVVAVADCVEEIGDSVTSLQDSIRELDSINYKDSAKFEMVMSDVET 256

Query: 144 WVSAALTDETTCSEGFQGKTVNGGVKGVVRTKIVNIAQLTSNALSLIN 189
           WVSAALT++ TC +GF    V   VK +VR  +V +A+LTSNAL+LIN
Sbjct: 257 WVSAALTNDDTCMDGF--SLVKTAVKDLVRRHVVEVARLTSNALALIN 302

[Close]
BLAST of Cucsa.251580 vs. NCBI nr
Match: gi|778688522|ref|XP_011652768.1| (PREDICTED: 21 kDa protein-like [Cucumis sativus])

HSP 1 Score: 365.5 bits (937), Expect = 5.6e-98
Identity = 191/193 (98.96%), Postives = 192/193 (99.48%), Query Frame = 1 

Query: 2   GISNSKKSLILFQLILQLTLIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQT 61
           GISNSKKSLILFQLILQLTLIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQT
Sbjct: 44  GISNSKKSLILFQLILQLTLIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQT 103

Query: 62  SPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASIS 121
           SPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASIS
Sbjct: 104 SPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASIS 163

Query: 122 EMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKGVVRTKIVNIAQLTS 181
           EMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKG VRTKIVNIAQLTS
Sbjct: 164 EMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKGAVRTKIVNIAQLTS 223

Query: 182 NALSLINQIGDLH 195
           NALSLIN+IGDLH
Sbjct: 224 NALSLINRIGDLH 236

[Close]
BLAST of Cucsa.251580 vs. NCBI nr
Match: gi|778679325|ref|XP_004148138.2| (PREDICTED: 21 kDa protein [Cucumis sativus])

HSP 1 Score: 364.0 bits (933), Expect = 1.6e-97
Identity = 190/194 (97.94%), Postives = 192/194 (98.97%), Query Frame = 1 

Query: 1   MGISNSKKSLILFQLILQLTLIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ 60
           MGISNSKK+LILFQLILQLTLIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ
Sbjct: 1   MGISNSKKTLILFQLILQLTLIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ 60

Query: 61  TSPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASI 120
           TSPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASI
Sbjct: 61  TSPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASI 120

Query: 121 SEMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKGVVRTKIVNIAQLT 180
           SEMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKG VRTKIVNIAQLT
Sbjct: 121 SEMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKGAVRTKIVNIAQLT 180

Query: 181 SNALSLINQIGDLH 195
           SNALSLIN+I DLH
Sbjct: 181 SNALSLINRIADLH 194

[Close]
BLAST of Cucsa.251580 vs. NCBI nr
Match: gi|659133745|ref|XP_008466883.1| (PREDICTED: 21 kDa protein-like, partial [Cucumis melo])

HSP 1 Score: 220.7 bits (561), Expect = 2.2e-54
Identity = 117/122 (95.90%), Postives = 119/122 (97.54%), Query Frame = 1 

Query: 1   MGISNSKKSLILFQLILQLTLIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ 60
           MGISN KKSLILFQLILQLT+IHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ
Sbjct: 1   MGISNCKKSLILFQLILQLTIIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ 60

Query: 61  TSPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASI 120
           TSPRLLATAALSVSLSSVKST TQILKLSHSHGL SRDVSAL+DCLEELSDSVDSLAASI
Sbjct: 61  TSPRLLATAALSVSLSSVKSTTTQILKLSHSHGLSSRDVSALNDCLEELSDSVDSLAASI 120

Query: 121 SE 123
           SE
Sbjct: 121 SE 122

[Close]
BLAST of Cucsa.251580 vs. NCBI nr
Match: gi|590671750|ref|XP_007038418.1| (DC1.2-like, putative [Theobroma cacao])

HSP 1 Score: 220.3 bits (560), Expect = 2.9e-54
Identity = 114/194 (58.76%), Postives = 146/194 (75.26%), Query Frame = 1 

Query: 4   SNSKKSLILFQLILQLTLIHSA---ITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ 63
           S+S K L++   I       SA   I PQSS EFI++SCSSTTYP+LCF +LS HA+ IQ
Sbjct: 7   SHSFKFLLILLAINSYINFSSAGRQIAPQSSAEFIRTSCSSTTYPKLCFETLSTHASLIQ 66

Query: 64  TSPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASI 123
           TSP+LLA AAL+V+LS+ +ST+  ++ LS SHGL  R+  A+ DC+EELSDS+D L  SI
Sbjct: 67  TSPQLLAHAALNVTLSTTESTSAMMVTLSKSHGLKPREAEAMQDCVEELSDSIDELRKSI 126

Query: 124 SEMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKGVVRTKIVNIAQLT 183
           SEM +++G+NF L +++VQTWVSAALTDE+TCS+GFQG  +NG VK  VRTKI+ IA LT
Sbjct: 127 SEMGQVKGSNFGLMINDVQTWVSAALTDESTCSDGFQGNNMNGNVKTAVRTKILRIAHLT 186

Query: 184 SNALSLINQIGDLH 195
           SNAL+LIN    LH
Sbjct: 187 SNALALINNYASLH 200

[Close]
BLAST of Cucsa.251580 vs. NCBI nr
Match: gi|596006342|ref|XP_007218426.1| (hypothetical protein PRUPE_ppa011607mg [Prunus persica])

HSP 1 Score: 214.2 bits (544), Expect = 2.1e-52
Identity = 108/179 (60.34%), Postives = 139/179 (77.65%), Query Frame = 1 

Query: 16  ILQLTLIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQTSPRLLATAALSVSL 75
           I+  T     + P+++TEFIK+SCS+TTYP+LC +SLS HA+ IQTSP+L+A AAL+V+L
Sbjct: 24  IISSTSAARDLAPKTNTEFIKTSCSATTYPKLCLTSLSSHASTIQTSPKLMAGAALNVTL 83

Query: 76  SSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASISEMPKLRGTNFDLAM 135
           SS KST+  +LKLS SHGL  ++V A+ DCLEELSDSVD L +SI+EM   +  +F L +
Sbjct: 84  SSAKSTSAVMLKLSQSHGLKPKEVGAMRDCLEELSDSVDELQSSIAEMGNFKSYDFQLMI 143

Query: 136 SNVQTWVSAALTDETTCSEGFQGKTVNGGVKGVVRTKIVNIAQLTSNALSLINQIGDLH 195
           S+VQTWVSAALTDE TCSEGF G  +NG +K  VR +IVNIAQLTSNAL+LIN+   +H
Sbjct: 144 SDVQTWVSAALTDENTCSEGFGGNGMNGNLKTAVRGRIVNIAQLTSNALALINRYASVH 202

The following BLAST results are available for this feature:
BLAST of Cucsa.251580 vs. Swiss-Prot
Match NameE-valueIdentityDescription
21KD_DAUCA5.9e-3944.7421 kDa protein OS=Daucus carota PE=2 SV=1[more]
PME2_ARATH5.4e-1630.77Pectinesterase 2 OS=Arabidopsis thaliana GN=PME2 PE=2 SV=2[more]
PME1_CITSI2.7e-1530.43Pectinesterase 1 OS=Citrus sinensis GN=PECS-1.1 PE=2 SV=1[more]
PME3_CITSI2.7e-1530.43Pectinesterase 3 OS=Citrus sinensis PE=1 SV=1[more]
PME34_ARATH1.3e-1428.78Probable pectinesterase/pectinesterase inhibitor 34 OS=Arabidopsis thaliana GN=P... [more]
BLAST of Cucsa.251580 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0L8D1_CUCSA3.9e-9898.96Uncharacterized protein OS=Cucumis sativus GN=Csa_3G175740 PE=4 SV=1[more]
A0A0A0LB27_CUCSA1.1e-9797.94Uncharacterized protein OS=Cucumis sativus GN=Csa_3G176245 PE=4 SV=1[more]
A0A061G793_THECC2.0e-5458.76DC1.2-like, putative OS=Theobroma cacao GN=TCM_014946 PE=4 SV=1[more]
M5XKR0_PRUPE1.5e-5260.34Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011607mg PE=4 SV=1[more]
B9R9R0_RICCO1.6e-5154.3621 kDa protein, putative OS=Ricinus communis GN=RCOM_1500140 PE=4 SV=1[more]
BLAST of Cucsa.251580 vs. TAIR10
Match NameE-valueIdentityDescription
AT5G62360.11.9e-4851.63 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT5G62350.11.1e-4048.92 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT4G25260.14.3e-4045.96 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT3G47380.11.0e-3646.47 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT1G62760.16.1e-3444.05 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
BLAST of Cucsa.251580 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|778688522|ref|XP_011652768.1|5.6e-9898.96PREDICTED: 21 kDa protein-like [Cucumis sativus][more]
gi|778679325|ref|XP_004148138.2|1.6e-9797.94PREDICTED: 21 kDa protein [Cucumis sativus][more]
gi|659133745|ref|XP_008466883.1|2.2e-5495.90PREDICTED: 21 kDa protein-like, partial [Cucumis melo][more]
gi|590671750|ref|XP_007038418.1|2.9e-5458.76DC1.2-like, putative [Theobroma cacao][more]
gi|596006342|ref|XP_007218426.1|2.1e-5260.34hypothetical protein PRUPE_ppa011607mg [Prunus persica][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR006501Pectinesterase_inhib_dom
Vocabulary: Molecular Function
TermDefinition
GO:0004857enzyme inhibitor activity
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0004857 enzyme inhibitor activity
molecular_function GO:0030599 pectinesterase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.251580.1Cucsa.251580.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainGENE3DG3DSA:1.20.140.40coord: 31..190
score: 8.9
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 34..186
score: 2.2
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 30..186
score: 3.4
IPR006501Pectinesterase inhibitor domainTIGRFAMsTIGR01614TIGR01614coord: 7..190
score: 1.7
IPR006501Pectinesterase inhibitor domainunknownSSF101148Plant invertase/pectin methylesterase inhibitorcoord: 33..190
score: 8.89
NoneNo IPR availablePANTHERPTHR31080FAMILY NOT NAMEDcoord: 1..194
score: 2.0
NoneNo IPR availablePANTHERPTHR31080:SF19PLANT INVERTASE/PECTIN METHYLESTERASE INHIBITOR SUPERFAMILY PROTEINcoord: 1..194
score: 2.0

Synteny
The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cucsa.251580Watermelon (97103) v2cgywmbB444
Cucsa.251580Watermelon (97103) v2cgywmbB452
Cucsa.251580Wax gourdcgywgoB522
Cucsa.251580Cucumber (Gy14) v1cgycgyB126
Cucsa.251580Cucurbita maxima (Rimu)cgycmaB0710
Cucsa.251580Cucurbita maxima (Rimu)cgycmaB0711
Cucsa.251580Cucurbita maxima (Rimu)cgycmaB0718
Cucsa.251580Cucurbita maxima (Rimu)cgycmaB0722
Cucsa.251580Cucurbita moschata (Rifu)cgycmoB0716
Cucsa.251580Cucurbita moschata (Rifu)cgycmoB0702
Cucsa.251580Cucurbita moschata (Rifu)cgycmoB0704
Cucsa.251580Cucurbita moschata (Rifu)cgycmoB0711
Cucsa.251580Wild cucumber (PI 183967)cgycpiB383
Cucsa.251580Wild cucumber (PI 183967)cgycpiB386
Cucsa.251580Wild cucumber (PI 183967)cgycpiB388
Cucsa.251580Cucumber (Chinese Long) v2cgycuB366
Cucsa.251580Cucumber (Chinese Long) v2cgycuB368
Cucsa.251580Cucumber (Chinese Long) v2cgycuB370
Cucsa.251580Melon (DHL92) v3.5.1cgymeB428
Cucsa.251580Melon (DHL92) v3.5.1cgymeB439
Cucsa.251580Watermelon (Charleston Gray)cgywcgB445
Cucsa.251580Watermelon (Charleston Gray)cgywcgB447
Cucsa.251580Watermelon (Charleston Gray)cgywcgB451
Cucsa.251580Watermelon (97103) v1cgywmB479
Cucsa.251580Watermelon (97103) v1cgywmB480
Cucsa.251580Cucurbita pepo (Zucchini)cgycpeB0673
Cucsa.251580Cucurbita pepo (Zucchini)cgycpeB0681
Cucsa.251580Cucurbita pepo (Zucchini)cgycpeB0686
Cucsa.251580Bottle gourd (USVL1VR-Ls)cgylsiB411
Cucsa.251580Bottle gourd (USVL1VR-Ls)cgylsiB416
Cucsa.251580Melon (DHL92) v3.6.1cgymedB422
Cucsa.251580Melon (DHL92) v3.6.1cgymedB432
Cucsa.251580Silver-seed gourdcarcgyB0437
Cucsa.251580Silver-seed gourdcarcgyB0993
Cucsa.251580Silver-seed gourdcarcgyB1042
Cucsa.251580Cucumber (Chinese Long) v3cgycucB387
Cucsa.251580Cucumber (Chinese Long) v3cgycucB392
Cucsa.251580Cucumber (Chinese Long) v3cgycucB395