Cucsa.251570 (gene) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.251570
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	Plant invertase/pectin methylesterase inhibitor superfamily protein
Location	scaffold02229 : 613714 .. 614206 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CACAGAGTTCATAAGAAGCTCTTGCAGTTCCACCACTTACCCTCGTCTCTGTTTTTCATCTCTTTCAGTCCATGCCAATGCCATCCAAACAAGTCCCCGCCTTTTGGCCACCGCCGCCCTCTCCGTTTCCCTCTCCTCCGTCAAGTCCACCGCCACTCAAATCCTCAAGCTTTCTCACTCCCATGGCCTCCCCTCCAGAGATGTTTCTGCTCTTAACGACTGTTTGGAAGAACTGAGTGACTCTGTCGACTCCCTCGCTGCTTCCATTTCCGAGATGCCGAAGCTCCGTGGAACCAATTTCGACCTCGCAATGAGTAATGTTCAGACATGGGTTAGCGCCGCTCTGACGGATGAAACCACATGTAGCGAAGGGTTTCAGGGGAAGACGGTGAACGGAGGCGTTAAGGCTGAGGTTAGGACTAAGATTGTCAACATTGCGCAGTTGACTAGTAATGCTCTGTCTTTGATTAATCGGATAGCTGATTTGCATTAG

mRNA sequence

CACAGAGTTCATAAGAAGCTCTTGCAGTTCCACCACTTACCCTCGTCTCTGTTTTTCATCTCTTTCAGTCCATGCCAATGCCATCCAAACAAGTCCCCGCCTTTTGGCCACCGCCGCCCTCTCCGTTTCCCTCTCCTCCGTCAAGTCCACCGCCACTCAAATCCTCAAGCTTTCTCACTCCCATGGCCTCCCCTCCAGAGATGTTTCTGCTCTTAACGACTGTTTGGAAGAACTGAGTGACTCTGTCGACTCCCTCGCTGCTTCCATTTCCGAGATGCCGAAGCTCCGTGGAACCAATTTCGACCTCGCAATGAGTAATGTTCAGACATGGGTTAGCGCCGCTCTGACGGATGAAACCACATGTAGCGAAGGGTTTCAGGGGAAGACGGTGAACGGAGGCGTTAAGGCTGAGGTTAGGACTAAGATTGTCAACATTGCGCAGTTGACTAGTAATGCTCTGTCTTTGATTAATCGGATAGCTGATTTGCATTAG

Coding sequence (CDS)

CACAGAGTTCATAAGAAGCTCTTGCAGTTCCACCACTTACCCTCGTCTCTGTTTTTCATCTCTTTCAGTCCATGCCAATGCCATCCAAACAAGTCCCCGCCTTTTGGCCACCGCCGCCCTCTCCGTTTCCCTCTCCTCCGTCAAGTCCACCGCCACTCAAATCCTCAAGCTTTCTCACTCCCATGGCCTCCCCTCCAGAGATGTTTCTGCTCTTAACGACTGTTTGGAAGAACTGAGTGACTCTGTCGACTCCCTCGCTGCTTCCATTTCCGAGATGCCGAAGCTCCGTGGAACCAATTTCGACCTCGCAATGAGTAATGTTCAGACATGGGTTAGCGCCGCTCTGACGGATGAAACCACATGTAGCGAAGGGTTTCAGGGGAAGACGGTGAACGGAGGCGTTAAGGCTGAGGTTAGGACTAAGATTGTCAACATTGCGCAGTTGACTAGTAATGCTCTGTCTTTGATTAATCGGATAGCTGATTTGCATTAG

Protein sequence

TEFIRSSCSSTTYPRLCFSSLSVHANAIQTSPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALNDCLEELSDSVDSLAASISEMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKAEVRTKIVNIAQLTSNALSLINRIADLH*

Homology

[Close]

BLAST of Cucsa.251570 vs. Swiss-Prot
Match: 21KD_DAUCA (21 kDa protein OS=Daucus carota PE=2 SV=1)

HSP 1 Score: 159.5 bits (402), Expect = 3.2e-38
Identity = 79/165 (47.88%), Postives = 115/165 (69.70%), Query Frame = 1


Query: 2   EFIRSSCSSTTYPRLCFSSLSVHANAIQTSPRLLATAALSVSLSSVKSTATQILKLSHSH 61
           +FI++SC+ TTYP +C  SLS +A  IQ +P+ LA+ AL VSL+  +   T + +L+   
Sbjct: 27  QFIKTSCTLTTYPAVCEQSLSAYAKTIQNNPQELASTALQVSLTRTQQAQTFMKRLNKFK 86

Query: 62  GLPSRDVSALNDCLEELSDSVDSLAASISEMPKL---RGTNFDLAMSNVQTWVSAALTDE 121
           GL +R  +A++DCLEE+ DS+D ++ S  EM  L   +G +F   MSNV+TWVSAALTDE
Sbjct: 87  GLKARQYAAIHDCLEEVEDSLDRVSRSCDEMKNLSHAKGNDFTFRMSNVETWVSAALTDE 146

Query: 122 TTCSEGFQGKTVNGGVKAEVRTKIVNIAQLTSNALSLINRIADLH 164
           TTC +GF GK ++G +K  VR ++V +A++TSNAL+L+N  A  H
Sbjct: 147 TTCMDGFAGKGMDGKIKESVRAQVVAVARVTSNALALVNNFAAKH 191

[Close]

BLAST of Cucsa.251570 vs. Swiss-Prot
Match: PME2_ARATH (Pectinesterase 2 OS=Arabidopsis thaliana GN=PME2 PE=2 SV=2)

HSP 1 Score: 84.0 bits (206), Expect = 1.7e-15
Identity = 46/160 (28.75%), Postives = 93/160 (58.13%), Query Frame = 1


Query: 4   IRSSCSSTTYPRLCFSSLSVHANAIQTSPRLLATAALSVSLSSVKSTATQILKL-SHSHG 63
           ++S CSST YP LCFS+++       TS + +  A+L+++  +VK     + KL +   G
Sbjct: 62  LKSVCSSTLYPELCFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKG 121

Query: 64  LPSRDVSALNDCLEELSDSVDSLAASISEMPKL-RGTNFDLAMSNVQTWVSAALTDETTC 123
           L  R+V+AL+DCLE + +++D L  ++ ++ +  +  +      +++T +S+A+T++ TC
Sbjct: 122 LTPREVTALHDCLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITNQGTC 181

Query: 124 SEGFQGKTVNGGVKAEVRTKIVNIAQLTSNALSLINRIAD 162
            +GF     +  V+  +    V++  + SNAL++I  + +
Sbjct: 182 LDGFSYDDADRKVRKALLKGQVHVEHMCSNALAMIKNMTE 221

[Close]

BLAST of Cucsa.251570 vs. Swiss-Prot
Match: PME1_CITSI (Pectinesterase 1 OS=Citrus sinensis GN=PECS-1.1 PE=2 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 2.9e-15
Identity = 48/161 (29.81%), Postives = 92/161 (57.14%), Query Frame = 1


Query: 4   IRSSCSSTTYPRLCFSSLSVHANAIQ--TSPRLLATAALSVSLSSVKSTATQILKLSHSH 63
           ++SSCSST YP LCFS+++    A +  TS + +   +L+++ ++V+     I KL    
Sbjct: 69  LKSSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRT 128

Query: 64  GLPSRDVSALNDCLEELSDSVDSLAASISEMPKLRGT-NFDLAMSNVQTWVSAALTDETT 123
            L  R+  AL+DCLE + +++D L  ++ ++ +     +      +++T +SAA+T++ T
Sbjct: 129 NLTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGT 188

Query: 124 CSEGFQGKTVNGGVKAEVRTKIVNIAQLTSNALSLINRIAD 162
           C +GF     N  V+  +    V++ ++ SNAL++I  + D
Sbjct: 189 CLDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTD 229

[Close]

BLAST of Cucsa.251570 vs. Swiss-Prot
Match: PME3_CITSI (Pectinesterase 3 OS=Citrus sinensis PE=1 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 2.9e-15
Identity = 48/161 (29.81%), Postives = 92/161 (57.14%), Query Frame = 1


Query: 4   IRSSCSSTTYPRLCFSSLSVHANAIQ--TSPRLLATAALSVSLSSVKSTATQILKLSHSH 63
           ++SSCSST YP LCFS+++    A +  TS + +   +L+++ ++V+     I KL    
Sbjct: 69  LKSSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRT 128

Query: 64  GLPSRDVSALNDCLEELSDSVDSLAASISEMPKLRGT-NFDLAMSNVQTWVSAALTDETT 123
            L  R+  AL+DCLE + +++D L  ++ ++ +     +      +++T +SAA+T++ T
Sbjct: 129 NLTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGT 188

Query: 124 CSEGFQGKTVNGGVKAEVRTKIVNIAQLTSNALSLINRIAD 162
           C +GF     N  V+  +    V++ ++ SNAL++I  + D
Sbjct: 189 CLDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTD 229

[Close]

BLAST of Cucsa.251570 vs. Swiss-Prot
Match: PME3_PHAVU (Pectinesterase 3 OS=Phaseolus vulgaris GN=MPE3 PE=2 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 1.1e-14
Identity = 53/162 (32.72%), Postives = 85/162 (52.47%), Query Frame = 1


Query: 4   IRSSCSSTTYPRLCFSSLSVHANAIQTSPRLLATAALSVSLSSVKSTATQILKLSHSHGL 63
           +++ C +T YP  CFSS+S    +  T P LL   +L V++  + S  +   KL  +   
Sbjct: 77  LKAVCDTTRYPSSCFSSISSLPESNTTDPELLFKLSLRVAIDELSSFPS---KLRANAEQ 136

Query: 64  PSRDVSALNDCLEELSDSVDSLAASISEMPKLRGTNFDLA-MSNVQTWVSAALTDETTCS 123
            +R   A++ C     D++D L  SIS +  + G     A +SNV+TW+SAALTD+ TC 
Sbjct: 137 DARLQKAIDVCSSVFGDALDRLNDSISALGTVAGRIASSASVSNVETWLSAALTDQDTCL 196

Query: 124 E--GFQGKTVNGGVKAEVRTKIVNIAQLTSNALSLINRIADL 163
           +  G    T   G   E+ T + N  +  SN+L+++ +I  L
Sbjct: 197 DAVGELNSTAARGALQEIETAMRNSTEFASNSLAIVTKILGL 235

[Close]

BLAST of Cucsa.251570 vs. TrEMBL
Match: A0A0A0LB27_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G176245 PE=4 SV=1)

HSP 1 Score: 306.6 bits (784), Expect = 1.8e-80
Identity = 159/163 (97.55%), Postives = 161/163 (98.77%), Query Frame = 1


Query: 1   TEFIRSSCSSTTYPRLCFSSLSVHANAIQTSPRLLATAALSVSLSSVKSTATQILKLSHS 60
           TEFI+SSCSSTTYPRLCFSSLSVHANAIQTSPRLLATAALSVSLSSVKSTATQILKLSHS
Sbjct: 32  TEFIKSSCSSTTYPRLCFSSLSVHANAIQTSPRLLATAALSVSLSSVKSTATQILKLSHS 91

Query: 61  HGLPSRDVSALNDCLEELSDSVDSLAASISEMPKLRGTNFDLAMSNVQTWVSAALTDETT 120
           HGLPSRDVSAL+DCLEELSDSVDSLAASISEMPKLRGTNFDLAMSNVQTWVSAALTDETT
Sbjct: 92  HGLPSRDVSALDDCLEELSDSVDSLAASISEMPKLRGTNFDLAMSNVQTWVSAALTDETT 151

Query: 121 CSEGFQGKTVNGGVKAEVRTKIVNIAQLTSNALSLINRIADLH 164
           CSEGFQGKTVNGGVK  VRTKIVNIAQLTSNALSLINRIADLH
Sbjct: 152 CSEGFQGKTVNGGVKGAVRTKIVNIAQLTSNALSLINRIADLH 194

[Close]

BLAST of Cucsa.251570 vs. TrEMBL
Match: A0A0A0L8D1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G175740 PE=4 SV=1)

HSP 1 Score: 305.1 bits (780), Expect = 5.3e-80
Identity = 158/163 (96.93%), Postives = 160/163 (98.16%), Query Frame = 1


Query: 1   TEFIRSSCSSTTYPRLCFSSLSVHANAIQTSPRLLATAALSVSLSSVKSTATQILKLSHS 60
           TEFI+SSCSSTTYPRLCFSSLSVHANAIQTSPRLLATAALSVSLSSVKSTATQILKLSHS
Sbjct: 74  TEFIKSSCSSTTYPRLCFSSLSVHANAIQTSPRLLATAALSVSLSSVKSTATQILKLSHS 133

Query: 61  HGLPSRDVSALNDCLEELSDSVDSLAASISEMPKLRGTNFDLAMSNVQTWVSAALTDETT 120
           HGLPSRDVSAL+DCLEELSDSVDSLAASISEMPKLRGTNFDLAMSNVQTWVSAALTDETT
Sbjct: 134 HGLPSRDVSALDDCLEELSDSVDSLAASISEMPKLRGTNFDLAMSNVQTWVSAALTDETT 193

Query: 121 CSEGFQGKTVNGGVKAEVRTKIVNIAQLTSNALSLINRIADLH 164
           CSEGFQGKTVNGGVK  VRTKIVNIAQLTSNALSLINRI DLH
Sbjct: 194 CSEGFQGKTVNGGVKGAVRTKIVNIAQLTSNALSLINRIGDLH 236

[Close]

BLAST of Cucsa.251570 vs. TrEMBL
Match: A0A061G793_THECC (DC1.2-like, putative OS=Theobroma cacao GN=TCM_014946 PE=4 SV=1)

HSP 1 Score: 213.0 bits (541), Expect = 2.7e-52
Identity = 104/162 (64.20%), Postives = 132/162 (81.48%), Query Frame = 1


Query: 2   EFIRSSCSSTTYPRLCFSSLSVHANAIQTSPRLLATAALSVSLSSVKSTATQILKLSHSH 61
           EFIR+SCSSTTYP+LCF +LS HA+ IQTSP+LLA AAL+V+LS+ +ST+  ++ LS SH
Sbjct: 39  EFIRTSCSSTTYPKLCFETLSTHASLIQTSPQLLAHAALNVTLSTTESTSAMMVTLSKSH 98

Query: 62  GLPSRDVSALNDCLEELSDSVDSLAASISEMPKLRGTNFDLAMSNVQTWVSAALTDETTC 121
           GL  R+  A+ DC+EELSDS+D L  SISEM +++G+NF L +++VQTWVSAALTDE+TC
Sbjct: 99  GLKPREAEAMQDCVEELSDSIDELRKSISEMGQVKGSNFGLMINDVQTWVSAALTDESTC 158

Query: 122 SEGFQGKTVNGGVKAEVRTKIVNIAQLTSNALSLINRIADLH 164
           S+GFQG  +NG VK  VRTKI+ IA LTSNAL+LIN  A LH
Sbjct: 159 SDGFQGNNMNGNVKTAVRTKILRIAHLTSNALALINNYASLH 200

[Close]

BLAST of Cucsa.251570 vs. TrEMBL
Match: M5XKR0_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011607mg PE=4 SV=1)

HSP 1 Score: 212.2 bits (539), Expect = 4.7e-52
Identity = 106/163 (65.03%), Postives = 132/163 (80.98%), Query Frame = 1


Query: 1   TEFIRSSCSSTTYPRLCFSSLSVHANAIQTSPRLLATAALSVSLSSVKSTATQILKLSHS 60
           TEFI++SCS+TTYP+LC +SLS HA+ IQTSP+L+A AAL+V+LSS KST+  +LKLS S
Sbjct: 40  TEFIKTSCSATTYPKLCLTSLSSHASTIQTSPKLMAGAALNVTLSSAKSTSAVMLKLSQS 99

Query: 61  HGLPSRDVSALNDCLEELSDSVDSLAASISEMPKLRGTNFDLAMSNVQTWVSAALTDETT 120
           HGL  ++V A+ DCLEELSDSVD L +SI+EM   +  +F L +S+VQTWVSAALTDE T
Sbjct: 100 HGLKPKEVGAMRDCLEELSDSVDELQSSIAEMGNFKSYDFQLMISDVQTWVSAALTDENT 159

Query: 121 CSEGFQGKTVNGGVKAEVRTKIVNIAQLTSNALSLINRIADLH 164
           CSEGF G  +NG +K  VR +IVNIAQLTSNAL+LINR A +H
Sbjct: 160 CSEGFGGNGMNGNLKTAVRGRIVNIAQLTSNALALINRYASVH 202

[Close]

BLAST of Cucsa.251570 vs. TrEMBL
Match: B9R9R0_RICCO (21 kDa protein, putative OS=Ricinus communis GN=RCOM_1500140 PE=4 SV=1)

HSP 1 Score: 208.8 bits (530), Expect = 5.2e-51
Identity = 102/163 (62.58%), Postives = 131/163 (80.37%), Query Frame = 1


Query: 1   TEFIRSSCSSTTYPRLCFSSLSVHANAIQTSPRLLATAALSVSLSSVKSTATQILKLSHS 60
           TEFIR+SCS+TTYPRLC+SSL +HA+ IQTSP LLA AAL+V+L+S +ST+T + KLS S
Sbjct: 38  TEFIRTSCSTTTYPRLCYSSLKIHASKIQTSPMLLANAALNVTLASTRSTSTMMQKLSKS 97

Query: 61  HGLPSRDVSALNDCLEELSDSVDSLAASISEMPKLRGTNFDLAMSNVQTWVSAALTDETT 120
           HG+  R+VSA+ DC++EL+DSVD L  SI E+ K +G+ F L M++VQTWVSAALTDE+T
Sbjct: 98  HGMKPREVSAMQDCMDELTDSVDELRKSIDELGKAQGSKFGLMMNDVQTWVSAALTDEST 157

Query: 121 CSEGFQGKTVNGGVKAEVRTKIVNIAQLTSNALSLINRIADLH 164
           CS+GF G T+NG +K  VR + V IA LTSNALSL+N  A +H
Sbjct: 158 CSDGFAGSTMNGELKTLVRQQTVKIAHLTSNALSLVNSYASVH 200

[Close]

BLAST of Cucsa.251570 vs. TAIR10
Match: AT5G62360.1 (AT5G62360.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 185.3 bits (469), Expect = 3.1e-47
Identity = 90/163 (55.21%), Postives = 130/163 (79.75%), Query Frame = 1


Query: 1   TEFIRSSCSSTTYPRLCFSSLSVHANAIQTSPRLLATAALSVSLSSVKSTATQILKLSHS 60
           TEF++SSC+ TTYPRLCFSSLS HA+ IQTSP+L+A AAL+++L+S K T+  +++LS+S
Sbjct: 41  TEFVKSSCTFTTYPRLCFSSLSTHASLIQTSPKLMAHAALNITLASAKVTSAMMVRLSNS 100

Query: 61  HGLPSRDVSALNDCLEELSDSVDSLAASISEMPKLRGTNFDLAMSNVQTWVSAALTDETT 120
             L  ++VSA+ DC+EEL D+++ L  SI EM +L G+N+++ +S++QTWVSAALTD  T
Sbjct: 101 R-LKPKEVSAMRDCVEELGDTLEELRKSIGEMCQLSGSNYEVYISDIQTWVSAALTDVNT 160

Query: 121 CSEGFQGKTVNGGVKAEVRTKIVNIAQLTSNALSLINRIADLH 164
           C++GF+G+ ++G VK  VR +I+ IA LTSNAL+LIN  A +H
Sbjct: 161 CTDGFEGEDMDGKVKVLVRGRILVIAHLTSNALALINHFASIH 202

[Close]

BLAST of Cucsa.251570 vs. TAIR10
Match: AT5G62350.1 (AT5G62350.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 162.9 bits (411), Expect = 1.6e-40
Identity = 85/163 (52.15%), Postives = 113/163 (69.33%), Query Frame = 1


Query: 3   FIRSSCSSTTYPRLCFSSLSVHANAIQTSPRLLATAALSVSLSSVKSTATQILKLSHSHG 62
           FI+SSC +TTYP LC  SLSV+AN IQTSP+ LA  A++V+LS  +ST   + +L+   G
Sbjct: 38  FIQSSCKTTTYPALCVHSLSVYANDIQTSPKRLAETAIAVTLSRAQSTKLFVSRLTRMKG 97

Query: 63  LPSRDVSALNDCLEELSDSVDSLAASISEMPKLRGT-----NFDLAMSNVQTWVSAALTD 122
           L  R+V A+ DC+EE++D+VD L  S+ E+ KL G+      F   MSN QTW SAALTD
Sbjct: 98  LKKREVEAIKDCVEEMNDTVDRLTKSVQEL-KLCGSAKDQDQFAYHMSNAQTWTSAALTD 157

Query: 123 ETTCSEGFQGKTVNGGVKAEVRTKIVNIAQLTSNALSLINRIA 161
           E TCS+GF G+ ++G +K  VR +I+N+   TSNALSLIN  A
Sbjct: 158 ENTCSDGFSGRVMDGRIKNSVRARIMNVGHETSNALSLINAFA 199

[Close]

BLAST of Cucsa.251570 vs. TAIR10
Match: AT4G25260.1 (AT4G25260.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 154.1 bits (388), Expect = 7.6e-38
Identity = 79/167 (47.31%), Postives = 112/167 (67.07%), Query Frame = 1


Query: 1   TEFIRSSCSSTTYPRLCFSSLSVHANAIQTSPRLLATAALSVSLSSVKSTATQILKLSHS 60
           TEFI++SC +T+YP  CF SLS +A+ I+  PR LA  AL+VS++  KS  T + +++  
Sbjct: 35  TEFIKASCETTSYPDRCFQSLSSYASEIKKQPRKLAETALAVSIARAKSAKTYVSEMTDY 94

Query: 61  HGLPSRDVSALNDCLEELSDSVDSLAASISEMPKLR----GTNFDLAMSNVQTWVSAALT 120
            G+  R   A+ DCLEE+ D+VD L+ S+ E+  L     G +F   +SNV+TW SAALT
Sbjct: 95  KGITKRQHEAVADCLEEMGDTVDRLSNSLKELKHLEEGDSGEDFWFCLSNVRTWTSAALT 154

Query: 121 DETTCSEGFQGKTVNGGVKAEVRTKIVNIAQLTSNALSLINRIADLH 164
           DET C +GF GK + G +K+ +RT IV++A+ TSNAL+LIN  A  H
Sbjct: 155 DETACMDGFGGKAMAGELKSLIRTHIVSVAEETSNALALINDFASKH 201

[Close]

BLAST of Cucsa.251570 vs. TAIR10
Match: AT3G47380.1 (AT3G47380.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 147.1 bits (370), Expect = 9.3e-36
Identity = 77/163 (47.24%), Postives = 111/163 (68.10%), Query Frame = 1


Query: 3   FIRSSCSSTTYPRLCFSSLSVHANAIQTSPRLLATAALSVSLSSVKSTATQILKLSHSHG 62
           FI++SC +TTYP +C +SL+ +AN+IQTSPR LA  AL+V+++  +ST   + +L     
Sbjct: 38  FIQASCKATTYPTVCVNSLTGYANSIQTSPRRLAETALNVTVTQAQSTKVFVWRLGRFTS 97

Query: 63  LPSRDVSALNDCLEELSDSVDSLAASISEMPKLRGT-----NFDLAMSNVQTWVSAALTD 122
           L  R++ A+ DC+EE+ D+VD L  SI E+ K+ G+      F   MSN QTW SAALT+
Sbjct: 98  LKKREIQAVKDCIEEIHDAVDRLTMSIHEV-KMCGSAKGRDQFWFHMSNAQTWTSAALTN 157

Query: 123 ETTCSEGFQGKTVNGGVKAEVRTKIVNIAQLTSNALSLINRIA 161
             TCS+GF G+ ++G VK  VR +I+N+ + TSNAL+LIN  A
Sbjct: 158 ANTCSDGFAGRVMDGRVKNSVRARILNLGRGTSNALALINAFA 199

[Close]

BLAST of Cucsa.251570 vs. TAIR10
Match: AT1G62760.1 (AT1G62760.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 136.0 bits (341), Expect = 2.1e-32
Identity = 72/162 (44.44%), Postives = 109/162 (67.28%), Query Frame = 1


Query: 2   EFIRSSCSSTTYPRLCFSSLSVHANAIQTSPRLLATAALSVSLSSVKSTATQILKLSHSH 61
           ++I++SC+ T Y  +C++SLS +A+ I+++P+ LA  AL+++LSS KS +  +  +SH  
Sbjct: 146 DYIKTSCNITLYKTICYNSLSPYASTIRSNPQKLAVIALNLTLSSAKSASKFVKNISHGG 205

Query: 62  GLPSRDVSALNDCLEELSDSVDSLAASISEMPKLR---GTNFDLAMSNVQTWVSAALTDE 121
           GL   +V A+ DC+EE+ DSV SL  SI E+  +       F++ MS+V+TWVSAALT++
Sbjct: 206 GLTRLEVVAVADCVEEIGDSVTSLQDSIRELDSINYKDSAKFEMVMSDVETWVSAALTND 265

Query: 122 TTCSEGFQGKTVNGGVKAEVRTKIVNIAQLTSNALSLINRIA 161
            TC +GF    V   VK  VR  +V +A+LTSNAL+LIN  A
Sbjct: 266 DTCMDGF--SLVKTAVKDLVRRHVVEVARLTSNALALINMYA 305

[Close]

BLAST of Cucsa.251570 vs. NCBI nr
Match: gi|778679325|ref|XP_004148138.2| (PREDICTED: 21 kDa protein [Cucumis sativus])

HSP 1 Score: 306.6 bits (784), Expect = 2.6e-80
Identity = 159/163 (97.55%), Postives = 161/163 (98.77%), Query Frame = 1


Query: 1   TEFIRSSCSSTTYPRLCFSSLSVHANAIQTSPRLLATAALSVSLSSVKSTATQILKLSHS 60
           TEFI+SSCSSTTYPRLCFSSLSVHANAIQTSPRLLATAALSVSLSSVKSTATQILKLSHS
Sbjct: 32  TEFIKSSCSSTTYPRLCFSSLSVHANAIQTSPRLLATAALSVSLSSVKSTATQILKLSHS 91

Query: 61  HGLPSRDVSALNDCLEELSDSVDSLAASISEMPKLRGTNFDLAMSNVQTWVSAALTDETT 120
           HGLPSRDVSAL+DCLEELSDSVDSLAASISEMPKLRGTNFDLAMSNVQTWVSAALTDETT
Sbjct: 92  HGLPSRDVSALDDCLEELSDSVDSLAASISEMPKLRGTNFDLAMSNVQTWVSAALTDETT 151

Query: 121 CSEGFQGKTVNGGVKAEVRTKIVNIAQLTSNALSLINRIADLH 164
           CSEGFQGKTVNGGVK  VRTKIVNIAQLTSNALSLINRIADLH
Sbjct: 152 CSEGFQGKTVNGGVKGAVRTKIVNIAQLTSNALSLINRIADLH 194

[Close]

BLAST of Cucsa.251570 vs. NCBI nr
Match: gi|778688522|ref|XP_011652768.1| (PREDICTED: 21 kDa protein-like [Cucumis sativus])

HSP 1 Score: 305.1 bits (780), Expect = 7.6e-80
Identity = 158/163 (96.93%), Postives = 160/163 (98.16%), Query Frame = 1


Query: 1   TEFIRSSCSSTTYPRLCFSSLSVHANAIQTSPRLLATAALSVSLSSVKSTATQILKLSHS 60
           TEFI+SSCSSTTYPRLCFSSLSVHANAIQTSPRLLATAALSVSLSSVKSTATQILKLSHS
Sbjct: 74  TEFIKSSCSSTTYPRLCFSSLSVHANAIQTSPRLLATAALSVSLSSVKSTATQILKLSHS 133

Query: 61  HGLPSRDVSALNDCLEELSDSVDSLAASISEMPKLRGTNFDLAMSNVQTWVSAALTDETT 120
           HGLPSRDVSAL+DCLEELSDSVDSLAASISEMPKLRGTNFDLAMSNVQTWVSAALTDETT
Sbjct: 134 HGLPSRDVSALDDCLEELSDSVDSLAASISEMPKLRGTNFDLAMSNVQTWVSAALTDETT 193

Query: 121 CSEGFQGKTVNGGVKAEVRTKIVNIAQLTSNALSLINRIADLH 164
           CSEGFQGKTVNGGVK  VRTKIVNIAQLTSNALSLINRI DLH
Sbjct: 194 CSEGFQGKTVNGGVKGAVRTKIVNIAQLTSNALSLINRIGDLH 236

[Close]

BLAST of Cucsa.251570 vs. NCBI nr
Match: gi|590671750|ref|XP_007038418.1| (DC1.2-like, putative [Theobroma cacao])

HSP 1 Score: 213.0 bits (541), Expect = 3.9e-52
Identity = 104/162 (64.20%), Postives = 132/162 (81.48%), Query Frame = 1


Query: 2   EFIRSSCSSTTYPRLCFSSLSVHANAIQTSPRLLATAALSVSLSSVKSTATQILKLSHSH 61
           EFIR+SCSSTTYP+LCF +LS HA+ IQTSP+LLA AAL+V+LS+ +ST+  ++ LS SH
Sbjct: 39  EFIRTSCSSTTYPKLCFETLSTHASLIQTSPQLLAHAALNVTLSTTESTSAMMVTLSKSH 98

Query: 62  GLPSRDVSALNDCLEELSDSVDSLAASISEMPKLRGTNFDLAMSNVQTWVSAALTDETTC 121
           GL  R+  A+ DC+EELSDS+D L  SISEM +++G+NF L +++VQTWVSAALTDE+TC
Sbjct: 99  GLKPREAEAMQDCVEELSDSIDELRKSISEMGQVKGSNFGLMINDVQTWVSAALTDESTC 158

Query: 122 SEGFQGKTVNGGVKAEVRTKIVNIAQLTSNALSLINRIADLH 164
           S+GFQG  +NG VK  VRTKI+ IA LTSNAL+LIN  A LH
Sbjct: 159 SDGFQGNNMNGNVKTAVRTKILRIAHLTSNALALINNYASLH 200

[Close]

BLAST of Cucsa.251570 vs. NCBI nr
Match: gi|596006342|ref|XP_007218426.1| (hypothetical protein PRUPE_ppa011607mg [Prunus persica])

HSP 1 Score: 212.2 bits (539), Expect = 6.7e-52
Identity = 106/163 (65.03%), Postives = 132/163 (80.98%), Query Frame = 1


Query: 1   TEFIRSSCSSTTYPRLCFSSLSVHANAIQTSPRLLATAALSVSLSSVKSTATQILKLSHS 60
           TEFI++SCS+TTYP+LC +SLS HA+ IQTSP+L+A AAL+V+LSS KST+  +LKLS S
Sbjct: 40  TEFIKTSCSATTYPKLCLTSLSSHASTIQTSPKLMAGAALNVTLSSAKSTSAVMLKLSQS 99

Query: 61  HGLPSRDVSALNDCLEELSDSVDSLAASISEMPKLRGTNFDLAMSNVQTWVSAALTDETT 120
           HGL  ++V A+ DCLEELSDSVD L +SI+EM   +  +F L +S+VQTWVSAALTDE T
Sbjct: 100 HGLKPKEVGAMRDCLEELSDSVDELQSSIAEMGNFKSYDFQLMISDVQTWVSAALTDENT 159

Query: 121 CSEGFQGKTVNGGVKAEVRTKIVNIAQLTSNALSLINRIADLH 164
           CSEGF G  +NG +K  VR +IVNIAQLTSNAL+LINR A +H
Sbjct: 160 CSEGFGGNGMNGNLKTAVRGRIVNIAQLTSNALALINRYASVH 202

[Close]

BLAST of Cucsa.251570 vs. NCBI nr
Match: gi|1009122319|ref|XP_015877940.1| (PREDICTED: 21 kDa protein-like [Ziziphus jujuba])

HSP 1 Score: 210.3 bits (534), Expect = 2.5e-51
Identity = 100/163 (61.35%), Postives = 137/163 (84.05%), Query Frame = 1


Query: 1   TEFIRSSCSSTTYPRLCFSSLSVHANAIQTSPRLLATAALSVSLSSVKSTATQILKLSHS 60
           T+FI++SCS T+YP+LCF+SLS HANAIQ SP+LLA  AL+V+LSS KST+  ++ LS S
Sbjct: 38  TDFIKTSCSVTSYPKLCFTSLSAHANAIQASPQLLAGTALNVTLSSAKSTSNLMVLLSRS 97

Query: 61  HGLPSRDVSALNDCLEELSDSVDSLAASISEMPKLRGTNFDLAMSNVQTWVSAALTDETT 120
            G+  R+V+A+ DC+EELSDSVD L  SI+EM +L+GTNF+L ++++QTWVSAALTDE+T
Sbjct: 98  QGINPREVAAMKDCIEELSDSVDELGKSINEMGQLKGTNFNLVINDIQTWVSAALTDEST 157

Query: 121 CSEGFQGKTVNGGVKAEVRTKIVNIAQLTSNALSLINRIADLH 164
           C++GF GK++NG +K  V+ +IVN+AQLTSNAL+LIN+ A +H
Sbjct: 158 CTDGFAGKSMNGNLKITVKDRIVNVAQLTSNALALINKFALIH 200

The following BLAST results are available for this feature:

BLAST of Cucsa.251570 vs. Swiss-Prot


Match Name	E-value	Identity	Description
21KD_DAUCA	3.2e-38	47.88	21 kDa protein OS=Daucus carota PE=2 SV=1	[more]
PME2_ARATH	1.7e-15	28.75	Pectinesterase 2 OS=Arabidopsis thaliana GN=PME2 PE=2 SV=2	[more]
PME1_CITSI	2.9e-15	29.81	Pectinesterase 1 OS=Citrus sinensis GN=PECS-1.1 PE=2 SV=1	[more]
PME3_CITSI	2.9e-15	29.81	Pectinesterase 3 OS=Citrus sinensis PE=1 SV=1	[more]
PME3_PHAVU	1.1e-14	32.72	Pectinesterase 3 OS=Phaseolus vulgaris GN=MPE3 PE=2 SV=1	[more]

BLAST of Cucsa.251570 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LB27_CUCSA	1.8e-80	97.55	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G176245 PE=4 SV=1	[more]
A0A0A0L8D1_CUCSA	5.3e-80	96.93	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G175740 PE=4 SV=1	[more]
A0A061G793_THECC	2.7e-52	64.20	DC1.2-like, putative OS=Theobroma cacao GN=TCM_014946 PE=4 SV=1	[more]
M5XKR0_PRUPE	4.7e-52	65.03	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011607mg PE=4 SV=1	[more]
B9R9R0_RICCO	5.2e-51	62.58	21 kDa protein, putative OS=Ricinus communis GN=RCOM_1500140 PE=4 SV=1	[more]

BLAST of Cucsa.251570 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G62360.1	3.1e-47	55.21	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G62350.1	1.6e-40	52.15	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT4G25260.1	7.6e-38	47.31	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT3G47380.1	9.3e-36	47.24	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G62760.1	2.1e-32	44.44	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

BLAST of Cucsa.251570 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778679325\|ref\|XP_004148138.2\|	2.6e-80	97.55	PREDICTED: 21 kDa protein [Cucumis sativus]	[more]
gi\|778688522\|ref\|XP_011652768.1\|	7.6e-80	96.93	PREDICTED: 21 kDa protein-like [Cucumis sativus]	[more]
gi\|590671750\|ref\|XP_007038418.1\|	3.9e-52	64.20	DC1.2-like, putative [Theobroma cacao]	[more]
gi\|596006342\|ref\|XP_007218426.1\|	6.7e-52	65.03	hypothetical protein PRUPE_ppa011607mg [Prunus persica]	[more]
gi\|1009122319\|ref\|XP_015877940.1\|	2.5e-51	61.35	PREDICTED: 21 kDa protein-like [Ziziphus jujuba]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR006501	Pectinesterase_inhib_dom

Vocabulary: **Molecular Function**
Term	Definition
GO:0004857	enzyme inhibitor activity

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0005982	starch metabolic process
biological_process	GO:0005985	sucrose metabolic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004857	enzyme inhibitor activity
molecular_function	GO:0030599	pectinesterase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsa.251570.1	Cucsa.251570.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	GENE3D	G3DSA:1.20.140.40		coord: 2..159 score: 4.2
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 3..155 score: 2.0
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 1..155 score: 3.9
IPR006501	Pectinesterase inhibitor domain	TIGRFAMs	TIGR01614	TIGR01614	coord: 2..158 score: 2.2
IPR006501	Pectinesterase inhibitor domain	unknown	SSF101148	Plant invertase/pectin methylesterase inhibitor	coord: 2..159 score: 3.01
None	No IPR available	PANTHER	PTHR31080	FAMILY NOT NAMED	coord: 1..163 score: 1.2
None	No IPR available	PANTHER	PTHR31080:SF19	PLANT INVERTASE/PECTIN METHYLESTERASE INHIBITOR SUPERFAMILY PROTEIN	coord: 1..163 score: 1.2

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cucsa.251570	MELO3C006819	Melon (DHL92) v3.5.1	cgymeB439
Cucsa.251570	MELO3C006819.2	Melon (DHL92) v3.6.1	cgymedB432

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cucsa.251570	Melon (DHL92) v3.6.1	cgymedB422
Cucsa.251570	Silver-seed gourd	carcgyB0437
Cucsa.251570	Silver-seed gourd	carcgyB0993
Cucsa.251570	Silver-seed gourd	carcgyB1042
Cucsa.251570	Cucumber (Chinese Long) v3	cgycucB387
Cucsa.251570	Cucumber (Chinese Long) v3	cgycucB392
Cucsa.251570	Cucumber (Chinese Long) v3	cgycucB395
Cucsa.251570	Watermelon (97103) v2	cgywmbB444
Cucsa.251570	Watermelon (97103) v2	cgywmbB452
Cucsa.251570	Wax gourd	cgywgoB522
Cucsa.251570	Cucumber (Gy14) v1	cgycgyB126
Cucsa.251570	Cucurbita maxima (Rimu)	cgycmaB0710
Cucsa.251570	Cucurbita maxima (Rimu)	cgycmaB0711
Cucsa.251570	Cucurbita maxima (Rimu)	cgycmaB0718
Cucsa.251570	Cucurbita maxima (Rimu)	cgycmaB0722
Cucsa.251570	Cucurbita moschata (Rifu)	cgycmoB0716
Cucsa.251570	Cucurbita moschata (Rifu)	cgycmoB0702
Cucsa.251570	Cucurbita moschata (Rifu)	cgycmoB0704
Cucsa.251570	Cucurbita moschata (Rifu)	cgycmoB0711
Cucsa.251570	Wild cucumber (PI 183967)	cgycpiB383
Cucsa.251570	Wild cucumber (PI 183967)	cgycpiB386
Cucsa.251570	Wild cucumber (PI 183967)	cgycpiB388
Cucsa.251570	Cucumber (Chinese Long) v2	cgycuB366
Cucsa.251570	Cucumber (Chinese Long) v2	cgycuB368
Cucsa.251570	Cucumber (Chinese Long) v2	cgycuB370
Cucsa.251570	Melon (DHL92) v3.5.1	cgymeB428
Cucsa.251570	Watermelon (Charleston Gray)	cgywcgB445
Cucsa.251570	Watermelon (Charleston Gray)	cgywcgB447
Cucsa.251570	Watermelon (Charleston Gray)	cgywcgB451
Cucsa.251570	Watermelon (97103) v1	cgywmB479
Cucsa.251570	Watermelon (97103) v1	cgywmB480
Cucsa.251570	Cucurbita pepo (Zucchini)	cgycpeB0673
Cucsa.251570	Cucurbita pepo (Zucchini)	cgycpeB0681
Cucsa.251570	Cucurbita pepo (Zucchini)	cgycpeB0686
Cucsa.251570	Bottle gourd (USVL1VR-Ls)	cgylsiB411
Cucsa.251570	Bottle gourd (USVL1VR-Ls)	cgylsiB416