Cucsa.251250 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.251250 vs. Swiss-Prot
Match: SBT13_ARATH (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana GN=SBT1.3 PE=2 SV=1)

HSP 1 Score: 1146.0 bits (2963), Expect = 0.0e+00
Identity = 544/739 (73.61%), Postives = 642/739 (86.87%), Query Frame = 1

Query: 1   MDRSAMPDSFTNHFEWYSNVLTNVVLDLQREGNGGGGEERIIYGYHNVFHGVAARLSEEE 60
           MD+SAMP  +TNH +WYS+ + +V     +E  G     RI+Y Y   FHG+AA+L++EE
Sbjct: 41  MDKSAMPLPYTNHLQWYSSKINSVTQHKSQEEEGNNN--RILYTYQTAFHGLAAQLTQEE 100

Query: 61  VEKLEEEDGVVAIFPEMKYELHTTRSPRFLGLEPADSNSAWSQQIADHDVVVGVLDTGIW 120
            E+LEEEDGVVA+ PE +YELHTTRSP FLGLE  +S   W++++ DHDVVVGVLDTGIW
Sbjct: 101 AERLEEEDGVVAVIPETRYELHTTRSPTFLGLERQESERVWAERVTDHDVVVGVLDTGIW 160

Query: 121 PESDSFDDAGMSPVPAHWKGECETGRGFTKQNCNRKIVGARVFYRGYQAATGKFNEQLEY 180
           PES+SF+D GMSPVPA W+G CETG+ F K+NCNRKIVGARVFYRGY+AATGK +E+LEY
Sbjct: 161 PESESFNDTGMSPVPATWRGACETGKRFLKRNCNRKIVGARVFYRGYEAATGKIDEELEY 220

Query: 181 KSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGMAPGARIAAYKVCWIGGCFSSDI 240
           KSPRD+DGHGTHTAATVAGSPV GA+L G+AYGTARGMA  AR+AAYKVCW+GGCFSSDI
Sbjct: 221 KSPRDRDGHGTHTAATVAGSPVKGANLFGFAYGTARGMAQKARVAAYKVCWVGGCFSSDI 280

Query: 241 LSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLT 300
           LSAVD+AVADGV VLSISLGGGVS+Y RDSLS+A FGAMEMGVFVSCSAGNGGPDP+SLT
Sbjct: 281 LSAVDQAVADGVQVLSISLGGGVSTYSRDSLSIATFGAMEMGVFVSCSAGNGGPDPISLT 340

Query: 301 NVSPWITTVGASTMDRDFPAIVKLGDGRTITGVSLYRGRITIPENKQFPIVYMGSNSSSP 360
           NVSPWITTVGASTMDRDFPA VK+G  RT  GVSLY+GR  +P+NKQ+P+VY+G N+SSP
Sbjct: 341 NVSPWITTVGASTMDRDFPATVKIGTMRTFKGVSLYKGRTVLPKNKQYPLVYLGRNASSP 400

Query: 361 DPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKNAGGIGMILSNTAANGEELVAD 420
           DP+S CL+G LD   VAGKIVICDRG++PRVQKG VVK AGGIGM+L+NTA NGEELVAD
Sbjct: 401 DPTSFCLDGALDRRHVAGKIVICDRGVTPRVQKGQVVKRAGGIGMVLTNTATNGEELVAD 460

Query: 421 CHLVPAVAIGEREGKAIKQYALTNRRATATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLE 480
            H++PAVA+GE+EGK IKQYA+T+++ATA+L  LGTR+G+KPSPVVAAFSSRGPNFL+LE
Sbjct: 461 SHMLPAVAVGEKEGKLIKQYAMTSKKATASLEILGTRIGIKPSPVVAAFSSRGPNFLSLE 520

Query: 481 ILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHP 540
           ILKPDL+APGVNILAAWTG   PSSL++D RRVKFNILSGTSMSCPHVSGVAALIKS+HP
Sbjct: 521 ILKPDLLAPGVNILAAWTGDMAPSSLSSDPRRVKFNILSGTSMSCPHVSGVAALIKSRHP 580

Query: 541 DWSPSAIKSALMTTAYVHDNTYKPLKDSSAASPSSPYDHGAGHINPRKALDPGLVYEIQP 600
           DWSP+AIKSALMTTAYVHDN +KPL D+S A+PSSPYDHGAGHI+P +A DPGLVY+I P
Sbjct: 581 DWSPAAIKSALMTTAYVHDNMFKPLTDASGAAPSSPYDHGAGHIDPLRATDPGLVYDIGP 640

Query: 601 QDYFDFLCTQDLSPTQLKVFSKYSNRTCR-GLLPNPGDLNYPAISAVFPEKTTVTSLTLH 660
           Q+YF+FLCTQDLSP+QLKVF+K+SNRTC+  L  NPG+LNYPAISA+FPE T V ++TL 
Sbjct: 641 QEYFEFLCTQDLSPSQLKVFTKHSNRTCKHTLAKNPGNLNYPAISALFPENTHVKAMTLR 700

Query: 661 RTVTNVGPATSSYHAVVSPFKGATVKVEPESLNFTRRYEKVSYRITFVTKKRQSMPEFGG 720
           RTVTNVGP  SSY   VSPFKGA+V V+P++LNFT +++K+SY +TF T+ R   PEFGG
Sbjct: 701 RTVTNVGPHISSYKVSVSPFKGASVTVQPKTLNFTSKHQKLSYTVTFRTRFRMKRPEFGG 760

Query: 721 LIWKDGSHKVRSPIVITWL 739
           L+WK  +HKVRSP++ITWL
Sbjct: 761 LVWKSTTHKVRSPVIITWL 777

BLAST of Cucsa.251250 vs. Swiss-Prot
Match: SBT17_ARATH (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 797.3 bits (2058), Expect = 1.4e-229
Identity = 401/740 (54.19%), Postives = 513/740 (69.32%), Query Frame = 1

Query: 1   MDRSAMPDSFTNHFEWYSNVLTNVVLDLQREGNGGGGEERIIYGYHNVFHGVAARLSEEE 60
           M +S MP SF  H  WY + L ++                ++Y Y N  HG + RL++EE
Sbjct: 36  MAKSQMPSSFDLHSNWYDSSLRSI-----------SDSAELLYTYENAIHGFSTRLTQEE 95

Query: 61  VEKLEEEDGVVAIFPEMKYELHTTRSPRFLGLEPADSNSAWSQQIADHDVVVGVLDTGIW 120
            + L  + GV+++ PE +YELHTTR+P FLGL+   ++  + +  +  DVVVGVLDTG+W
Sbjct: 96  ADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTAD-LFPEAGSYSDVVVGVLDTGVW 155

Query: 121 PESDSFDDAGMSPVPAHWKGECETGRGFTKQNCNRKIVGARVFYRGYQAATGKFNEQLEY 180
           PES S+ D G  P+P+ WKG CE G  FT   CNRK++GAR F RGY++  G  +E  E 
Sbjct: 156 PESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYESTMGPIDESKES 215

Query: 181 KSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGMAPGARIAAYKVCWIGGCFSSDI 240
           +SPRD DGHGTHT++T AGS V GASLLGYA GTARGMAP AR+A YKVCW+GGCFSSDI
Sbjct: 216 RSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCWLGGCFSSDI 275

Query: 241 LSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLT 300
           L+A+D+A+AD VNVLS+SLGGG+S YYRD +++ AF AME G+ VSCSAGN GP   SL+
Sbjct: 276 LAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSLS 335

Query: 301 NVSPWITTVGASTMDRDFPAIVKLGDGRTITGVSLYRGRITIPENKQFPIVYMGSNSSSP 360
           NV+PWITTVGA T+DRDFPA+  LG+G+  TGVSL++G   +P+ K  P +Y G N+S+ 
Sbjct: 336 NVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGE-ALPD-KLLPFIYAG-NASNA 395

Query: 361 DPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKNAGGIGMILSNTAANGEELVAD 420
              +LC+ GTL P  V GKIV+CDRGI+ RVQKG VVK AGG+GMIL+NTAANGEELVAD
Sbjct: 396 TNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILANTAANGEELVAD 455

Query: 421 CHLVPAVAIGEREGKAIKQYALTNRRATATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLE 480
            HL+PA  +GE+ G  I+ Y  T+   TA++  LGT +GVKPSPVVAAFSSRGPN +T  
Sbjct: 456 AHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPN 515

Query: 481 ILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHP 540
           ILKPDL+APGVNILAAWTG  GP+ L +D+RRV+FNI+SGTSMSCPHVSG+AAL+KS HP
Sbjct: 516 ILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHP 575

Query: 541 DWSPSAIKSALMTTAYVHDNTYKPLKDSSAASPSSPYDHGAGHINPRKALDPGLVYEIQP 600
           +WSP+AI+SALMTTAY      KPL D +   PS+P+DHGAGH++P  A +PGL+Y++  
Sbjct: 576 EWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTTATNPGLIYDLTT 635

Query: 601 QDYFDFLCTQDLSPTQLKVFSKYSNRTC-RGLLPNPGDLNYPAISAVFPEKTTVTSLTLH 660
           +DY  FLC  + +  Q++  S+  N TC      +  DLNYP+ +        V +    
Sbjct: 636 EDYLGFLCALNYTSPQIRSVSR-RNYTCDPSKSYSVADLNYPSFAV---NVDGVGAYKYT 695

Query: 661 RTVTNVGPATSSYHAVVSPFKGATVKVEPESLNFTRRYEKVSYRITFV--TKKRQSMPEF 720
           RTVT+VG A +    V S   G  + VEP  LNF    EK SY +TF   + K      F
Sbjct: 696 RTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSNSF 755

Query: 721 GGLIWKDGSHKVRSPIVITW 738
           G + W DG H V SP+ I+W
Sbjct: 756 GSIEWSDGKHVVGSPVAISW 756

BLAST of Cucsa.251250 vs. Swiss-Prot
Match: SBT18_ARATH (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana GN=SBT1.8 PE=2 SV=1)

HSP 1 Score: 756.5 bits (1952), Expect = 2.7e-217
Identity = 384/745 (51.54%), Postives = 502/745 (67.38%), Query Frame = 1

Query: 1   MDRSAMPDSFTNHFEWYSNVLTNVVLDLQREGNGGGGEERIIYGYHNVFHGVAARLSEEE 60
           ++ S  P+SF  H +WY++ L +              E  ++Y Y   FHG +A L   E
Sbjct: 34  VNHSDKPESFLTHHDWYTSQLNS--------------ESSLLYTYTTSFHGFSAYLDSTE 93

Query: 61  VEKL-EEEDGVVAIFPEMKYELHTTRSPRFLGLEPADSNSAWSQQIADHDVVVGVLDTGI 120
            + L    + ++ IF +  Y LHTTR+P FLGL             + + V++GVLDTG+
Sbjct: 94  ADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLN--SEFGVHDLGSSSNGVIIGVLDTGV 153

Query: 121 WPESDSFDDAGMSPVPAHWKGECETGRGFTKQNCNRKIVGARVFYRGYQAATGK-FNEQL 180
           WPES SFDD  M  +P+ WKGECE+G  F  + CN+K++GAR F +G+Q A+G  F+ + 
Sbjct: 154 WPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGGFSSKR 213

Query: 181 EYKSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGMAPGARIAAYKVCWIGGCFSS 240
           E  SPRD DGHGTHT+ T AGS V  AS LGYA GTARGMA  AR+A YKVCW  GCF S
Sbjct: 214 ESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWSTGCFGS 273

Query: 241 DILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVS 300
           DIL+A+DRA+ DGV+VLS+SLGGG + YYRD++++ AF AME GVFVSCSAGN GP   S
Sbjct: 274 DILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCSAGNSGPTRAS 333

Query: 301 LTNVSPWITTVGASTMDRDFPAIVKLGDGRTITGVSLYRGRITIPENKQFPIVYMGSNSS 360
           + NV+PW+ TVGA T+DRDFPA   LG+G+ +TGVSLY G       K   +VY   NSS
Sbjct: 334 VANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSG--VGMGTKPLELVYNKGNSS 393

Query: 361 SPDPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKNAGGIGMILSNTAANGEELV 420
           S   S+LCL G+LD   V GKIV+CDRG++ RV+KG VV++AGG+GMI++NTAA+GEELV
Sbjct: 394 S---SNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAASGEELV 453

Query: 421 ADCHLVPAVAIGEREGKAIKQYALTNRRATATLGFLGTRLGVKPSPVVAAFSSRGPNFLT 480
           AD HL+PA+A+G++ G  +++Y  ++ + TA L F GT L VKPSPVVAAFSSRGPN +T
Sbjct: 454 ADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPVVAAFSSRGPNTVT 513

Query: 481 LEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSGTSMSCPHVSGVAALIKSK 540
            EILKPD++ PGVNILA W+   GP+ L  D+RR +FNI+SGTSMSCPH+SG+A L+K+ 
Sbjct: 514 PEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGLLKAA 573

Query: 541 HPDWSPSAIKSALMTTAYVHDNTYKPLKDSSAASPSSPYDHGAGHINPRKALDPGLVYEI 600
           HP+WSPSAIKSALMTTAYV DNT  PL D++  S S+PY HG+GH++P+KAL PGLVY+I
Sbjct: 574 HPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLVYDI 633

Query: 601 QPQDYFDFLCTQDLSPTQLKVFSKYSNRTCRGLLPNPGDLNYPAISAVFPEKTTVTSLTL 660
             ++Y  FLC+ D +   +    K  +  C     +PG LNYP+ S +F  K  V     
Sbjct: 634 STEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNYPSFSVLFGGKRVV---RY 693

Query: 661 HRTVTNVGPATSSYHAVVSPFKGATVKVEPESLNFTRRYEKVSYRITFVTKKRQSM---P 720
            R VTNVG A+S Y   V+      + V+P  L+F    EK  Y +TFV+KK  SM    
Sbjct: 694 TREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMTNKA 753

Query: 721 EFGGLIWKDGSHKVRSPIVITWLSF 741
           EFG + W +  H+VRSP+  +W  F
Sbjct: 754 EFGSITWSNPQHEVRSPVAFSWNRF 754

BLAST of Cucsa.251250 vs. Swiss-Prot
Match: SBT15_ARATH (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 747.3 bits (1928), Expect = 1.6e-214
Identity = 376/752 (50.00%), Postives = 511/752 (67.95%), Query Frame = 1

Query: 1   MDRSAMPDSFTNHFEWYSNVLTNVVLDLQREGNGGGGEERIIYGYHNVFHGVAARLSEEE 60
           +D  A P  F  HF WY++ L ++                II+ Y  VFHG +ARL+ ++
Sbjct: 32  VDHEAKPSIFPTHFHWYTSSLASLT----------SSPPSIIHTYDTVFHGFSARLTSQD 91

Query: 61  VEKLEEEDGVVAIFPEMKYELHTTRSPRFLGLEPADSNSAWSQQIADHDVVVGVLDTGIW 120
             +L +   V+++ PE    LHTTRSP FLGL   D      +     D+V+GV+DTG+W
Sbjct: 92  ASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEESDFGSDLVIGVIDTGVW 151

Query: 121 PESDSFDDAGMSPVPAHWKGECETGRGFTKQNCNRKIVGARVFYRGYQAATGKFNEQLEY 180
           PE  SFDD G+ PVP  WKG+C   + F +  CNRK+VGAR F  GY+A  GK NE  E+
Sbjct: 152 PERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCGGYEATNGKMNETTEF 211

Query: 181 KSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGMAPGARIAAYKVCWIGGCFSSDI 240
           +SPRD DGHGTHTA+  AG  V  AS LGYA+G A GMAP AR+AAYKVCW  GC+ SDI
Sbjct: 212 RSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVCWNSGCYDSDI 271

Query: 241 LSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLT 300
           L+A D AVADGV+V+S+S+GG V  YY D++++ AFGA++ G+FVS SAGNGGP  +++T
Sbjct: 272 LAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFVSASAGNGGPGALTVT 331

Query: 301 NVSPWITTVGASTMDRDFPAIVKLGDGRTITGVSLYRGRITIPENKQFPIVYMGSNSSSP 360
           NV+PW+TTVGA T+DRDFPA VKLG+G+ I+GVS+Y G    P  + +P+VY GS     
Sbjct: 332 NVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDP-GRMYPLVYGGSLLGGD 391

Query: 361 D-PSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKNAGGIGMILSNTAANGEELVA 420
              SSLCLEG+LDP+ V GKIV+CDRGI+ R  KG +V+  GG+GMI++N   +GE LVA
Sbjct: 392 GYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKNGGLGMIIANGVFDGEGLVA 451

Query: 421 DCHLVPAVAIGEREGKAIKQY------ALTNRRATATLGFLGTRLGVKPSPVVAAFSSRG 480
           DCH++PA ++G   G  I++Y      + +++  TAT+ F GTRLG++P+PVVA+FS+RG
Sbjct: 452 DCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLGIRPAPVVASFSARG 511

Query: 481 PNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSGTSMSCPHVSGVAA 540
           PN  T EILKPD++APG+NILAAW  + GPS +T+D RR +FNILSGTSM+CPHVSG+AA
Sbjct: 512 PNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILSGTSMACPHVSGLAA 571

Query: 541 LIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSSAASPSSPYDHGAGHINPRKALDPG 600
           L+K+ HPDWSP+AI+SAL+TTAY  DN+ +P+ D S  + SS  D+G+GH++P KA+DPG
Sbjct: 572 LLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNTSSVMDYGSGHVHPTKAMDPG 631

Query: 601 LVYEIQPQDYFDFLCTQDLSPTQLKVFSKYSNRTCRGL--LPNPGDLNYPAISAVFPEKT 660
           LVY+I   DY +FLC  + + T +   ++     C G     + G+LNYP+ S VF ++ 
Sbjct: 632 LVYDITSYDYINFLCNSNYTRTNIVTITR-RQADCDGARRAGHVGNLNYPSFSVVF-QQY 691

Query: 661 TVTSLTLH--RTVTNVGPATSSYHAVVSPFKGATVKVEPESLNFTRRYEKVSYRITFVTK 720
             + ++ H  RTVTNVG + S Y   + P +G TV VEPE L+F R  +K+S+ +   T 
Sbjct: 692 GESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFRRVGQKLSFVVRVKTT 751

Query: 721 KRQSMP-----EFGGLIWKDGSHKVRSPIVIT 737
           + +  P     E G ++W DG   V SP+V+T
Sbjct: 752 EVKLSPGATNVETGHIVWSDGKRNVTSPLVVT 770

BLAST of Cucsa.251250 vs. Swiss-Prot
Match: SBT16_ARATH (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana GN=SBT1.6 PE=2 SV=1)

HSP 1 Score: 744.6 bits (1921), Expect = 1.1e-213
Identity = 382/749 (51.00%), Postives = 504/749 (67.29%), Query Frame = 1

Query: 1   MDRSAMPDSFTNHFEWYSNVLTNVVLDLQREGNGGGGEERIIYGYHNVFHGVAARLSEEE 60
           +D  +MP  F  H+ WYS                   E RI++ YH VFHG +A ++ +E
Sbjct: 32  IDGGSMPSIFPTHYHWYSTEFAE--------------ESRIVHVYHTVFHGFSAVVTPDE 91

Query: 61  VEKLEEEDGVVAIFPEMKYELHTTRSPRFLGLEPADSNSAWSQQIADHDVVVGVLDTGIW 120
            + L     V+A+F + + ELHTTRSP+FLGL+  +    WS+     DV++GV DTGIW
Sbjct: 92  ADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQ--NQKGLWSESDYGSDVIIGVFDTGIW 151

Query: 121 PESDSFDDAGMSPVPAHWKGECETGRGFTKQNCNRKIVGARVFYRGYQAAT-GKFNEQLE 180
           PE  SF D  + P+P  W+G CE+G  F+ +NCNRKI+GAR F +G QAA  G  N+ +E
Sbjct: 152 PERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKIIGARFFAKGQQAAVIGGINKTVE 211

Query: 181 YKSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGMAPGARIAAYKVCWI-GGCFSS 240
           + SPRD DGHGTHT++T AG     AS+ GYA G A+G+AP ARIAAYKVCW   GC  S
Sbjct: 212 FLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAKGVAPKARIAAYKVCWKDSGCLDS 271

Query: 241 DILSAVDRAVADGVNVLSISLGGG---VSSYYRDSLSVAAFGAMEMGVFVSCSAGNGGPD 300
           DIL+A D AV DGV+V+SIS+GGG    S YY D +++ ++GA   G+FVS SAGN GP+
Sbjct: 272 DILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAIGSYGAASKGIFVSSSAGNEGPN 331

Query: 301 PVSLTNVSPWITTVGASTMDRDFPAIVKLGDGRTITGVSLYRGRITIPEN-KQFPIVYMG 360
            +S+TN++PW+TTVGAST+DR+FPA   LGDG  + GVSLY G   +P N + FP+VY G
Sbjct: 332 GMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRGVSLYAG---VPLNGRMFPVVYPG 391

Query: 361 SNSSSPDPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKNAGGIGMILSNTAANG 420
            +  S   +SLC+E TLDP  V GKIVICDRG SPRV KG+VVK AGG+GMIL+N A+NG
Sbjct: 392 KSGMSS--ASLCMENTLDPKQVRGKIVICDRGSSPRVAKGLVVKKAGGVGMILANGASNG 451

Query: 421 EELVADCHLVPAVAIGEREGKAIKQYALTNRRATATLGFLGTRLGVKPSPVVAAFSSRGP 480
           E LV D HL+PA A+G  EG  IK YA ++    A++ F GT +G+KP+PV+A+FS RGP
Sbjct: 452 EGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASIDFRGTIVGIKPAPVIASFSGRGP 511

Query: 481 NFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSGTSMSCPHVSGVAAL 540
           N L+ EILKPDL+APGVNILAAWT   GP+ L +D R+ +FNILSGTSM+CPHVSG AAL
Sbjct: 512 NGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNILSGTSMACPHVSGAAAL 571

Query: 541 IKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSSAASPSSPYDHGAGHINPRKALDPGL 600
           +KS HPDWSP+ I+SA+MTT  + DN+ + L D S    ++PYD+G+GH+N  +A++PGL
Sbjct: 572 LKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDESTGKSATPYDYGSGHLNLGRAMNPGL 631

Query: 601 VYEIQPQDYFDFLCTQDLSPTQLKVFSKYSNRTCRGLLPNPGDLNYPAISAVFP-EKTTV 660
           VY+I   DY  FLC+    P  ++V ++   R      P+PG+LNYP+I+AVFP  +  +
Sbjct: 632 VYDITNDDYITFLCSIGYGPKTIQVITRTPVRCPTTRKPSPGNLNYPSITAVFPTNRRGL 691

Query: 661 TSLTLHRTVTNVGPATSSYHAVVSPFKGATVKVEPESLNFTRRYEKVSYRITFVTKKR-- 720
            S T+ RT TNVG A + Y A +   +G TV V+P  L FT   ++ SY +T     R  
Sbjct: 692 VSKTVIRTATNVGQAEAVYRARIESPRGVTVTVKPPRLVFTSAVKRRSYAVTVTVNTRNV 751

Query: 721 ---QSMPEFGGLIWKD-GSHKVRSPIVIT 737
              ++   FG + W D G H VRSPIV+T
Sbjct: 752 VLGETGAVFGSVTWFDGGKHVVRSPIVVT 759

BLAST of Cucsa.251250 vs. TrEMBL
Match: A0A0A0LB64_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G178520 PE=4 SV=1)

HSP 1 Score: 1501.9 bits (3887), Expect = 0.0e+00
Identity = 741/741 (100.00%), Postives = 741/741 (100.00%), Query Frame = 1

Query: 1   MDRSAMPDSFTNHFEWYSNVLTNVVLDLQREGNGGGGEERIIYGYHNVFHGVAARLSEEE 60
           MDRSAMPDSFTNHFEWYSNVLTNVVLDLQREGNGGGGEERIIYGYHNVFHGVAARLSEEE
Sbjct: 67  MDRSAMPDSFTNHFEWYSNVLTNVVLDLQREGNGGGGEERIIYGYHNVFHGVAARLSEEE 126

Query: 61  VEKLEEEDGVVAIFPEMKYELHTTRSPRFLGLEPADSNSAWSQQIADHDVVVGVLDTGIW 120
           VEKLEEEDGVVAIFPEMKYELHTTRSPRFLGLEPADSNSAWSQQIADHDVVVGVLDTGIW
Sbjct: 127 VEKLEEEDGVVAIFPEMKYELHTTRSPRFLGLEPADSNSAWSQQIADHDVVVGVLDTGIW 186

Query: 121 PESDSFDDAGMSPVPAHWKGECETGRGFTKQNCNRKIVGARVFYRGYQAATGKFNEQLEY 180
           PESDSFDDAGMSPVPAHWKGECETGRGFTKQNCNRKIVGARVFYRGYQAATGKFNEQLEY
Sbjct: 187 PESDSFDDAGMSPVPAHWKGECETGRGFTKQNCNRKIVGARVFYRGYQAATGKFNEQLEY 246

Query: 181 KSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGMAPGARIAAYKVCWIGGCFSSDI 240
           KSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGMAPGARIAAYKVCWIGGCFSSDI
Sbjct: 247 KSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGMAPGARIAAYKVCWIGGCFSSDI 306

Query: 241 LSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLT 300
           LSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLT
Sbjct: 307 LSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLT 366

Query: 301 NVSPWITTVGASTMDRDFPAIVKLGDGRTITGVSLYRGRITIPENKQFPIVYMGSNSSSP 360
           NVSPWITTVGASTMDRDFPAIVKLGDGRTITGVSLYRGRITIPENKQFPIVYMGSNSSSP
Sbjct: 367 NVSPWITTVGASTMDRDFPAIVKLGDGRTITGVSLYRGRITIPENKQFPIVYMGSNSSSP 426

Query: 361 DPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKNAGGIGMILSNTAANGEELVAD 420
           DPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKNAGGIGMILSNTAANGEELVAD
Sbjct: 427 DPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKNAGGIGMILSNTAANGEELVAD 486

Query: 421 CHLVPAVAIGEREGKAIKQYALTNRRATATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLE 480
           CHLVPAVAIGEREGKAIKQYALTNRRATATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLE
Sbjct: 487 CHLVPAVAIGEREGKAIKQYALTNRRATATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLE 546

Query: 481 ILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHP 540
           ILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHP
Sbjct: 547 ILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHP 606

Query: 541 DWSPSAIKSALMTTAYVHDNTYKPLKDSSAASPSSPYDHGAGHINPRKALDPGLVYEIQP 600
           DWSPSAIKSALMTTAYVHDNTYKPLKDSSAASPSSPYDHGAGHINPRKALDPGLVYEIQP
Sbjct: 607 DWSPSAIKSALMTTAYVHDNTYKPLKDSSAASPSSPYDHGAGHINPRKALDPGLVYEIQP 666

Query: 601 QDYFDFLCTQDLSPTQLKVFSKYSNRTCRGLLPNPGDLNYPAISAVFPEKTTVTSLTLHR 660
           QDYFDFLCTQDLSPTQLKVFSKYSNRTCRGLLPNPGDLNYPAISAVFPEKTTVTSLTLHR
Sbjct: 667 QDYFDFLCTQDLSPTQLKVFSKYSNRTCRGLLPNPGDLNYPAISAVFPEKTTVTSLTLHR 726

Query: 661 TVTNVGPATSSYHAVVSPFKGATVKVEPESLNFTRRYEKVSYRITFVTKKRQSMPEFGGL 720
           TVTNVGPATSSYHAVVSPFKGATVKVEPESLNFTRRYEKVSYRITFVTKKRQSMPEFGGL
Sbjct: 727 TVTNVGPATSSYHAVVSPFKGATVKVEPESLNFTRRYEKVSYRITFVTKKRQSMPEFGGL 786

Query: 721 IWKDGSHKVRSPIVITWLSFV 742
           IWKDGSHKVRSPIVITWLSFV
Sbjct: 787 IWKDGSHKVRSPIVITWLSFV 807

BLAST of Cucsa.251250 vs. TrEMBL
Match: M5XPU8_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa1027166mg PE=4 SV=1)

HSP 1 Score: 1229.5 bits (3180), Expect = 0.0e+00
Identity = 583/739 (78.89%), Postives = 666/739 (90.12%), Query Frame = 1

Query: 1   MDRSAMPDSFTNHFEWYSNVLTNVVLDLQREGNGGGGEERIIYGYHNVFHGVAARLSEEE 60
           MD+SA P+SFTNH +WYS+ + ++V   + E +GG  +ER+IY Y N FHGVAARLSEEE
Sbjct: 39  MDKSAKPESFTNHLDWYSSKVNSIVFKPENEEDGGHDQERVIYTYQNAFHGVAARLSEEE 98

Query: 61  VEKLEEEDGVVAIFPEMKYELHTTRSPRFLGLEPADSNSA-WSQQIADHDVVVGVLDTGI 120
            E+L+E+DGV+AIFP+ KY+LHTTRSP FLGLEP DS +  WSQ++ DHDV+VGVLDTG+
Sbjct: 99  AERLQEQDGVLAIFPDTKYQLHTTRSPLFLGLEPHDSTTTVWSQRVTDHDVIVGVLDTGV 158

Query: 121 WPESDSFDDAGMSPVPAHWKGECETGRGFTKQNCNRKIVGARVFYRGYQAATGKFNEQLE 180
           WPES SF+D GMSPVPA+WKG CETGRGF+K NCN+KIVGAR+FY GY+AATGK NEQ E
Sbjct: 159 WPESQSFNDTGMSPVPAYWKGACETGRGFSKHNCNKKIVGARIFYHGYEAATGKINEQTE 218

Query: 181 YKSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGMAPGARIAAYKVCWIGGCFSSD 240
           +KSPRDQDGHGTHTAATVAGSPV GA+LLGYA+GTARGMAPGARIAAYKVCW+GGCFSSD
Sbjct: 219 FKSPRDQDGHGTHTAATVAGSPVRGANLLGYAHGTARGMAPGARIAAYKVCWVGGCFSSD 278

Query: 241 ILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSL 300
           ILSAVD+AVADGVNVLSISLGGGVS+YYRDSLS+AAFGAMEMGVFVSCSAGNGGPDPVSL
Sbjct: 279 ILSAVDKAVADGVNVLSISLGGGVSAYYRDSLSIAAFGAMEMGVFVSCSAGNGGPDPVSL 338

Query: 301 TNVSPWITTVGASTMDRDFPAIVKLGDGRTITGVSLYRGRITIPENKQFPIVYMGSNSSS 360
           TNVSPWITTVGASTMDRDFP+ VKLG+GRT+TGVSLY+GR+ +  NKQ+P+VYMG NS+S
Sbjct: 339 TNVSPWITTVGASTMDRDFPSSVKLGNGRTVTGVSLYKGRMMLSTNKQYPVVYMGDNSTS 398

Query: 361 PDPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKNAGGIGMILSNTAANGEELVA 420
           PDPSSLCLEGTLD   VAGKIVICDRGISPRVQKG VVK+AGG+GMIL+NTAANGEELVA
Sbjct: 399 PDPSSLCLEGTLDRRVVAGKIVICDRGISPRVQKGQVVKDAGGVGMILANTAANGEELVA 458

Query: 421 DCHLVPAVAIGEREGKAIKQYALTNRRATATLGFLGTRLGVKPSPVVAAFSSRGPNFLTL 480
           DCHLVPAVA+GE E KAIK YALT+ RATATL FLGTR GV+PSPVVAAFSSRGPNF++L
Sbjct: 459 DCHLVPAVAVGETEAKAIKHYALTSPRATATLAFLGTRTGVRPSPVVAAFSSRGPNFVSL 518

Query: 481 EILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSGTSMSCPHVSGVAALIKSKH 540
           EILKPD+VAPGVNILAAWTG  GPSSL TD RRVKFNILSGTSMSCPHVSG+AAL+K++H
Sbjct: 519 EILKPDVVAPGVNILAAWTGALGPSSLPTDHRRVKFNILSGTSMSCPHVSGIAALLKARH 578

Query: 541 PDWSPSAIKSALMTTAYVHDNTYKPLKDSSAASPSSPYDHGAGHINPRKALDPGLVYEIQ 600
           P+WSP+AIKSALMTTAYVHDNT+KPL+D+SAA  S+PYDHGAGHINPRKALDPGLVY+I+
Sbjct: 579 PEWSPAAIKSALMTTAYVHDNTHKPLQDASAAEASTPYDHGAGHINPRKALDPGLVYDIE 638

Query: 601 PQDYFDFLCTQDLSPTQLKVFSKYSNRTCRGLLPNPGDLNYPAISAVFPEKTTVTSLTLH 660
            QDY +FLCTQ L+P QLKVF+KYSNR+C+  L +PGDLNYPAIS VFPE+T V+ LTLH
Sbjct: 639 AQDYLEFLCTQRLTPMQLKVFTKYSNRSCKHALASPGDLNYPAISVVFPERTNVSLLTLH 698

Query: 661 RTVTNVGPATSSYHAVVSPFKGATVKVEPESLNFTRRYEKVSYRITFVTKKRQSMPEFGG 720
           RTVTNVGP  S+YHA+VSPFKGA VKVEP +L FTR  +K+SY+ITF TK RQ+ PEFGG
Sbjct: 699 RTVTNVGPPVSNYHAIVSPFKGAYVKVEPRTLKFTRANQKLSYKITFTTKSRQATPEFGG 758

Query: 721 LIWKDGSHKVRSPIVITWL 739
           L+WKDG H+VRSPIV+ WL
Sbjct: 759 LVWKDGVHRVRSPIVVVWL 777

BLAST of Cucsa.251250 vs. TrEMBL
Match: A0A061G0C3_THECC (Subtilase 1.3 OS=Theobroma cacao GN=TCM_015025 PE=4 SV=1)

HSP 1 Score: 1226.8 bits (3173), Expect = 0.0e+00
Identity = 593/740 (80.14%), Postives = 667/740 (90.14%), Query Frame = 1

Query: 1   MDRSAMPDSFTNHFEWYSNVLTNVVL-DLQREGNGGGGEERIIYGYHNVFHGVAARLSEE 60
           MD+SAMP SF++H EWYS+ + +V++ + Q EG+G G  ERIIY Y N FHGVAA+L+E+
Sbjct: 38  MDKSAMPASFSSHLEWYSSKVKSVIMSNTQSEGDGDG--ERIIYSYQNAFHGVAAQLTED 97

Query: 61  EVEKLEEEDGVVAIFPEMKYELHTTRSPRFLGLEPADSNSAWSQQIADHDVVVGVLDTGI 120
           E E+LEEEDGVVAI PEMKY+LHTTRSP FLGLEP +S S WSQ++ DHDV+VGVLDTGI
Sbjct: 98  EAERLEEEDGVVAILPEMKYQLHTTRSPMFLGLEPEESTSIWSQKLTDHDVIVGVLDTGI 157

Query: 121 WPESDSFDDAGMSPVPAHWKGECETGRGFTKQNCNRKIVGARVFYRGYQAATGKFNEQLE 180
           WPES+SF+D G++PVPAHWKG CETGRGF K +CNRKIVGARVFYRGY+AATGK NE+ E
Sbjct: 158 WPESESFNDTGLAPVPAHWKGACETGRGFEKHHCNRKIVGARVFYRGYEAATGKINEKNE 217

Query: 181 YKSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGMAPGARIAAYKVCWIGGCFSSD 240
           YKSPRDQDGHGTHTAATVAGSPV GA+LLGYAYGTARGMAPGARIAAYKVCW GGCFSSD
Sbjct: 218 YKSPRDQDGHGTHTAATVAGSPVRGANLLGYAYGTARGMAPGARIAAYKVCWTGGCFSSD 277

Query: 241 ILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSL 300
           ILSAVDRAVADGV+VLSISLGGGVSSYYRDSL++A FGAMEMGVFVSCSAGNGGPDPVSL
Sbjct: 278 ILSAVDRAVADGVSVLSISLGGGVSSYYRDSLAIATFGAMEMGVFVSCSAGNGGPDPVSL 337

Query: 301 TNVSPWITTVGASTMDRDFPAIVKLGDGRTITGVSLYRGRITIPENKQFPIVYMGSNSSS 360
           TNVSPWITTVGASTMDRDFPA VKLG GRT+TGVSLY+G+  +  NKQ+PIVYMGSNSSS
Sbjct: 338 TNVSPWITTVGASTMDRDFPADVKLGTGRTLTGVSLYKGQRFLSPNKQYPIVYMGSNSSS 397

Query: 361 PDPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKNAGGIGMILSNTAANGEELVA 420
           PDPSSLCLEGTLDPH V+GKIVICDRGISPRVQKG VVK+AGGIGMIL+NTAANGEELVA
Sbjct: 398 PDPSSLCLEGTLDPHIVSGKIVICDRGISPRVQKGQVVKDAGGIGMILTNTAANGEELVA 457

Query: 421 DCHLVPAVAIGEREGKAIKQYALTNRRATATLGFLGTRLGVKPSPVVAAFSSRGPNFLTL 480
           DCHL+PA+A+GE EGKAIK YALT+R+ATATL FLGTRLG++PSPVVAAFSSRGPNFLT 
Sbjct: 458 DCHLLPALAVGEMEGKAIKHYALTSRKATATLAFLGTRLGIRPSPVVAAFSSRGPNFLTF 517

Query: 481 EILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSGTSMSCPHVSGVAALIKSKH 540
           EILKPD+VAPGVNILAAWTG+ GPSSL TD RRVKFNILSGTSMSCPHVSG+AAL+K++H
Sbjct: 518 EILKPDMVAPGVNILAAWTGELGPSSLQTDHRRVKFNILSGTSMSCPHVSGIAALLKARH 577

Query: 541 PDWSPSAIKSALMTTAYVHDNTYKPLKDSSAASPSSPYDHGAGHINPRKALDPGLVYEIQ 600
           P+WSP+AIKSALMTTAYVHDNT+ PLKD++ A+ S+PYDHGAGHINP KALDPGLVY+I+
Sbjct: 578 PEWSPAAIKSALMTTAYVHDNTHNPLKDAAEAAISTPYDHGAGHINPLKALDPGLVYDIE 637

Query: 601 PQDYFDFLCTQDLSPTQLKVFSKYSNRTCRGLLPNPGDLNYPAISAVFPEKTTVTS-LTL 660
            QDYF+FLCTQ L+  QLKVF KYSNR C   L + GDLNYPAIS VFPE TT  S LTL
Sbjct: 638 AQDYFEFLCTQKLTTMQLKVFGKYSNRFCHHTLASAGDLNYPAISVVFPEDTTAISVLTL 697

Query: 661 HRTVTNVGPATSSYHAVVSPFKGATVKVEPESLNFTRRYEKVSYRITFVTKKRQSMPEFG 720
           HRTVTNVGP  S+YH VVS FKGATVKV+P+SLNFTR+ +K+SY+ITF TK  Q++PEFG
Sbjct: 698 HRTVTNVGPPISNYHVVVSQFKGATVKVDPKSLNFTRKNQKLSYKITFTTKSPQTVPEFG 757

Query: 721 GLIWKDGSHKVRSPIVITWL 739
           GL+WKDG HKVRSPIVITW+
Sbjct: 758 GLVWKDGVHKVRSPIVITWI 775

BLAST of Cucsa.251250 vs. TrEMBL
Match: B9R9K9_RICCO (Cucumisin, putative OS=Ricinus communis GN=RCOM_1498530 PE=4 SV=1)

HSP 1 Score: 1214.9 bits (3142), Expect = 0.0e+00
Identity = 582/739 (78.76%), Postives = 659/739 (89.17%), Query Frame = 1

Query: 1   MDRSAMPDSFTNHFEWYSNVLTNVVLDLQREGNGGGGEERIIYGYHNVFHGVAARLSEEE 60
           MD+ A P+SF+NH EWYS+ + +V+   + E +    +ERIIY Y  VFHGVAA+LSEEE
Sbjct: 36  MDKYAKPESFSNHLEWYSSKVQSVLSKSEHEADTDN-DERIIYSYQTVFHGVAAKLSEEE 95

Query: 61  VEKLEEEDGVVAIFPEMKYELHTTRSPRFLGLEPADSNSAWSQQIADHDVVVGVLDTGIW 120
            ++LEE DGVVAIFPE KY++HTTRSP FLGLEP DS S WSQ IADHDV+VGVLDTGIW
Sbjct: 96  AKRLEEADGVVAIFPETKYQIHTTRSPMFLGLEPQDSTSVWSQTIADHDVIVGVLDTGIW 155

Query: 121 PESDSFDDAGMSPVPAHWKGECETGRGFTKQNCNRKIVGARVFYRGYQAATGKFNEQLEY 180
           PES SF+D GM+ VPAHWKG CETGRGF K +CN+KIVGARVFY+GY+ ATGK NEQ EY
Sbjct: 156 PESASFNDTGMTTVPAHWKGTCETGRGFGKHHCNKKIVGARVFYKGYEVATGKINEQNEY 215

Query: 181 KSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGMAPGARIAAYKVCWIGGCFSSDI 240
           KSPRDQDGHGTHTAATVAGSPV  A+LLGYAYGTARGMAPGARIAAYKVCW GGCFSSDI
Sbjct: 216 KSPRDQDGHGTHTAATVAGSPVHDANLLGYAYGTARGMAPGARIAAYKVCWAGGCFSSDI 275

Query: 241 LSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLT 300
           LSAVDRAV+DGVNVLSISLGGGVSSYYRDSLS+AAFGAMEMG+FVSCSAGNGGPDP SLT
Sbjct: 276 LSAVDRAVSDGVNVLSISLGGGVSSYYRDSLSIAAFGAMEMGIFVSCSAGNGGPDPASLT 335

Query: 301 NVSPWITTVGASTMDRDFPAIVKLGDGRTITGVSLYRGRITIPENKQFPIVYMGSNSSSP 360
           NVSPWITTVGASTMDRDFPA V LG GRT+TGVSLY+GR T+  NKQ+P+VYMGSNSSSP
Sbjct: 336 NVSPWITTVGASTMDRDFPATVHLGTGRTLTGVSLYKGRRTLLTNKQYPLVYMGSNSSSP 395

Query: 361 DPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKNAGGIGMILSNTAANGEELVAD 420
           DPSSLCLEGTL+PH VAGKIVICDRGISPRVQKG V K+AG +GMIL+NTAANGEELVAD
Sbjct: 396 DPSSLCLEGTLNPHIVAGKIVICDRGISPRVQKGQVAKDAGAVGMILTNTAANGEELVAD 455

Query: 421 CHLVPAVAIGEREGKAIKQYALTNRRATATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLE 480
           CHL PAV++GEREGK IK YALT R A+ATL FLGT++G++PSPVVAAFSSRGPNFL+LE
Sbjct: 456 CHLFPAVSVGEREGKLIKHYALTRRNASATLAFLGTKVGIRPSPVVAAFSSRGPNFLSLE 515

Query: 481 ILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHP 540
           ILKPD+VAPGVNI+AAWTG+TGPSSL TD RRV+FNILSGTSMSCPHVSG+AAL+K++HP
Sbjct: 516 ILKPDVVAPGVNIIAAWTGETGPSSLPTDHRRVRFNILSGTSMSCPHVSGIAALLKARHP 575

Query: 541 DWSPSAIKSALMTTAYVHDNTYKPLKDSSAASPSSPYDHGAGHINPRKALDPGLVYEIQP 600
           +WSP+AIKSALMTTAYVHDNT KPL+D+S  +PSSPYDHGAGHINP KALDPGL+Y+I+ 
Sbjct: 576 EWSPAAIKSALMTTAYVHDNTQKPLQDASTDAPSSPYDHGAGHINPLKALDPGLIYDIEA 635

Query: 601 QDYFDFLCTQDLSPTQLKVFSKYSNRTCRGLLPNPGDLNYPAISAVFPEKTTVTSLTLHR 660
           QDYF+FLCTQ LS TQL+VF KY+NRTC+  L +PGDLNYPAISAVF +  T++SLTLHR
Sbjct: 636 QDYFEFLCTQRLSITQLRVFGKYANRTCQKSLLSPGDLNYPAISAVFTDSNTISSLTLHR 695

Query: 661 TVTNVGPATSSYHAVVSPFKGATVKVEPESLNFTRRYEKVSYRITFVTKKRQSMPEFGGL 720
           TVTNVGP TS+YHAVVS FKGATVK+EP++L FT + +K+SYRITF  K RQ MPEFGGL
Sbjct: 696 TVTNVGPPTSTYHAVVSRFKGATVKIEPKTLKFTAKNQKLSYRITFTAKSRQIMPEFGGL 755

Query: 721 IWKDGSHKVRSPIVITWLS 740
           +WKDG HKVRSPIV+TWL+
Sbjct: 756 VWKDGVHKVRSPIVLTWLT 773

BLAST of Cucsa.251250 vs. TrEMBL
Match: B9ICZ0_POPTR (Subtilase family protein OS=Populus trichocarpa GN=POPTR_0015s14110g PE=4 SV=2)

HSP 1 Score: 1213.7 bits (3139), Expect = 0.0e+00
Identity = 580/739 (78.48%), Postives = 657/739 (88.90%), Query Frame = 1

Query: 1   MDRSAMPDSFTNHFEWYSNVLTNVVLDLQREGNGGGGEERIIYGYHNVFHGVAARLSEEE 60
           MD+SA P+ FT+H EWYS+ + +V+ + Q EG+    E+RIIY Y   FHGVAA+L+EEE
Sbjct: 38  MDKSAKPEYFTSHLEWYSSKVQSVLSEPQGEGDADE-EDRIIYSYETAFHGVAAKLNEEE 97

Query: 61  VEKLEEEDGVVAIFPEMKYELHTTRSPRFLGLEPADSNSAWSQQIADHDVVVGVLDTGIW 120
             +LEE DGVVAIFPE KY+LHTTRSP FL LEP DS S WS+++ADHDV+VGVLDTGIW
Sbjct: 98  AARLEEADGVVAIFPETKYQLHTTRSPMFLRLEPEDSTSVWSEKLADHDVIVGVLDTGIW 157

Query: 121 PESDSFDDAGMSPVPAHWKGECETGRGFTKQNCNRKIVGARVFYRGYQAATGKFNEQLEY 180
           PES+SF+D G++ VP HWKG CETGR F K +CNRKIVGARVFYRGY+AATGK NEQ EY
Sbjct: 158 PESESFNDTGITAVPVHWKGICETGRAFQKHHCNRKIVGARVFYRGYEAATGKINEQNEY 217

Query: 181 KSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGMAPGARIAAYKVCWIGGCFSSDI 240
           KSPRDQDGHGTHTAATVAGSPV GA+LLGYAYGTARGMAPGARIAAYKVCW GGCFSSDI
Sbjct: 218 KSPRDQDGHGTHTAATVAGSPVRGANLLGYAYGTARGMAPGARIAAYKVCWAGGCFSSDI 277

Query: 241 LSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLT 300
           LSAVDRAVADGVNVLSISLGGGVSSYYRDSLS+AAFGAMEMGVFVSCSAGNGGP P SLT
Sbjct: 278 LSAVDRAVADGVNVLSISLGGGVSSYYRDSLSIAAFGAMEMGVFVSCSAGNGGPSPASLT 337

Query: 301 NVSPWITTVGASTMDRDFPAIVKLGDGRTITGVSLYRGRITIPENKQFPIVYMGSNSSSP 360
           NVSPWITTVGAS+MDRDFPA   +G G+TI+GVSLYRG+  +   KQ+P+VYMGSNSSSP
Sbjct: 338 NVSPWITTVGASSMDRDFPATAMIGTGKTISGVSLYRGQRILSTRKQYPLVYMGSNSSSP 397

Query: 361 DPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKNAGGIGMILSNTAANGEELVAD 420
           DPSSLCLEGTL+P  V+GKIVICDRGI+PRVQKG V K AG +GMILSNTAANGEELVAD
Sbjct: 398 DPSSLCLEGTLNPRVVSGKIVICDRGITPRVQKGQVAKEAGAVGMILSNTAANGEELVAD 457

Query: 421 CHLVPAVAIGEREGKAIKQYALTNRRATATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLE 480
           CHL+PAVA+GE+EGK IK YALT++ ATATL FLGTRLG+KPSPVVAAFSSRGPNFLTLE
Sbjct: 458 CHLLPAVAVGEKEGKLIKTYALTSQNATATLAFLGTRLGIKPSPVVAAFSSRGPNFLTLE 517

Query: 481 ILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHP 540
           ILKPD++APGVNILAAWTG  GPSSL TD RRVKFNILSGTSMSCPHVSG+AAL+K++HP
Sbjct: 518 ILKPDVLAPGVNILAAWTGDLGPSSLPTDHRRVKFNILSGTSMSCPHVSGIAALLKARHP 577

Query: 541 DWSPSAIKSALMTTAYVHDNTYKPLKDSSAASPSSPYDHGAGHINPRKALDPGLVYEIQP 600
           +WSP+AIKSALMTTAYVHDNT+ PLKD+SA +PS+PYDHGAGHINP KALDPGL+Y+I+P
Sbjct: 578 EWSPAAIKSALMTTAYVHDNTHNPLKDASATTPSTPYDHGAGHINPMKALDPGLIYDIEP 637

Query: 601 QDYFDFLCTQDLSPTQLKVFSKYSNRTCRGLLPNPGDLNYPAISAVFPEKTTVTSLTLHR 660
           QDYFDFLCTQ L+PTQLKVF KY+NR+CR  L NPGDLNYPAIS VFP+ T++  LTLHR
Sbjct: 638 QDYFDFLCTQKLTPTQLKVFGKYANRSCRHSLANPGDLNYPAISVVFPDDTSIKVLTLHR 697

Query: 661 TVTNVGPATSSYHAVVSPFKGATVKVEPESLNFTRRYEKVSYRITFVTKKRQSMPEFGGL 720
           TVTNVG  TS YHAV+SPFKGATVKVEPE LNFT + +K+SY+I F T+ RQ++PEFGGL
Sbjct: 698 TVTNVGLPTSKYHAVISPFKGATVKVEPEILNFTMKNQKLSYKIIFTTRTRQTIPEFGGL 757

Query: 721 IWKDGSHKVRSPIVITWLS 740
           +WKDG+HKVRSP+VITWL+
Sbjct: 758 VWKDGAHKVRSPVVITWLT 775

BLAST of Cucsa.251250 vs. TAIR10
Match: AT5G51750.1 (AT5G51750.1 subtilase 1.3)

HSP 1 Score: 1146.0 bits (2963), Expect = 0.0e+00
Identity = 544/739 (73.61%), Postives = 642/739 (86.87%), Query Frame = 1

Query: 1   MDRSAMPDSFTNHFEWYSNVLTNVVLDLQREGNGGGGEERIIYGYHNVFHGVAARLSEEE 60
           MD+SAMP  +TNH +WYS+ + +V     +E  G     RI+Y Y   FHG+AA+L++EE
Sbjct: 41  MDKSAMPLPYTNHLQWYSSKINSVTQHKSQEEEGNNN--RILYTYQTAFHGLAAQLTQEE 100

Query: 61  VEKLEEEDGVVAIFPEMKYELHTTRSPRFLGLEPADSNSAWSQQIADHDVVVGVLDTGIW 120
            E+LEEEDGVVA+ PE +YELHTTRSP FLGLE  +S   W++++ DHDVVVGVLDTGIW
Sbjct: 101 AERLEEEDGVVAVIPETRYELHTTRSPTFLGLERQESERVWAERVTDHDVVVGVLDTGIW 160

Query: 121 PESDSFDDAGMSPVPAHWKGECETGRGFTKQNCNRKIVGARVFYRGYQAATGKFNEQLEY 180
           PES+SF+D GMSPVPA W+G CETG+ F K+NCNRKIVGARVFYRGY+AATGK +E+LEY
Sbjct: 161 PESESFNDTGMSPVPATWRGACETGKRFLKRNCNRKIVGARVFYRGYEAATGKIDEELEY 220

Query: 181 KSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGMAPGARIAAYKVCWIGGCFSSDI 240
           KSPRD+DGHGTHTAATVAGSPV GA+L G+AYGTARGMA  AR+AAYKVCW+GGCFSSDI
Sbjct: 221 KSPRDRDGHGTHTAATVAGSPVKGANLFGFAYGTARGMAQKARVAAYKVCWVGGCFSSDI 280

Query: 241 LSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLT 300
           LSAVD+AVADGV VLSISLGGGVS+Y RDSLS+A FGAMEMGVFVSCSAGNGGPDP+SLT
Sbjct: 281 LSAVDQAVADGVQVLSISLGGGVSTYSRDSLSIATFGAMEMGVFVSCSAGNGGPDPISLT 340

Query: 301 NVSPWITTVGASTMDRDFPAIVKLGDGRTITGVSLYRGRITIPENKQFPIVYMGSNSSSP 360
           NVSPWITTVGASTMDRDFPA VK+G  RT  GVSLY+GR  +P+NKQ+P+VY+G N+SSP
Sbjct: 341 NVSPWITTVGASTMDRDFPATVKIGTMRTFKGVSLYKGRTVLPKNKQYPLVYLGRNASSP 400

Query: 361 DPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKNAGGIGMILSNTAANGEELVAD 420
           DP+S CL+G LD   VAGKIVICDRG++PRVQKG VVK AGGIGM+L+NTA NGEELVAD
Sbjct: 401 DPTSFCLDGALDRRHVAGKIVICDRGVTPRVQKGQVVKRAGGIGMVLTNTATNGEELVAD 460

Query: 421 CHLVPAVAIGEREGKAIKQYALTNRRATATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLE 480
            H++PAVA+GE+EGK IKQYA+T+++ATA+L  LGTR+G+KPSPVVAAFSSRGPNFL+LE
Sbjct: 461 SHMLPAVAVGEKEGKLIKQYAMTSKKATASLEILGTRIGIKPSPVVAAFSSRGPNFLSLE 520

Query: 481 ILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHP 540
           ILKPDL+APGVNILAAWTG   PSSL++D RRVKFNILSGTSMSCPHVSGVAALIKS+HP
Sbjct: 521 ILKPDLLAPGVNILAAWTGDMAPSSLSSDPRRVKFNILSGTSMSCPHVSGVAALIKSRHP 580

Query: 541 DWSPSAIKSALMTTAYVHDNTYKPLKDSSAASPSSPYDHGAGHINPRKALDPGLVYEIQP 600
           DWSP+AIKSALMTTAYVHDN +KPL D+S A+PSSPYDHGAGHI+P +A DPGLVY+I P
Sbjct: 581 DWSPAAIKSALMTTAYVHDNMFKPLTDASGAAPSSPYDHGAGHIDPLRATDPGLVYDIGP 640

Query: 601 QDYFDFLCTQDLSPTQLKVFSKYSNRTCR-GLLPNPGDLNYPAISAVFPEKTTVTSLTLH 660
           Q+YF+FLCTQDLSP+QLKVF+K+SNRTC+  L  NPG+LNYPAISA+FPE T V ++TL 
Sbjct: 641 QEYFEFLCTQDLSPSQLKVFTKHSNRTCKHTLAKNPGNLNYPAISALFPENTHVKAMTLR 700

Query: 661 RTVTNVGPATSSYHAVVSPFKGATVKVEPESLNFTRRYEKVSYRITFVTKKRQSMPEFGG 720
           RTVTNVGP  SSY   VSPFKGA+V V+P++LNFT +++K+SY +TF T+ R   PEFGG
Sbjct: 701 RTVTNVGPHISSYKVSVSPFKGASVTVQPKTLNFTSKHQKLSYTVTFRTRFRMKRPEFGG 760

Query: 721 LIWKDGSHKVRSPIVITWL 739
           L+WK  +HKVRSP++ITWL
Sbjct: 761 LVWKSTTHKVRSPVIITWL 777

BLAST of Cucsa.251250 vs. TAIR10
Match: AT5G67360.1 (AT5G67360.1 Subtilase family protein)

HSP 1 Score: 797.3 bits (2058), Expect = 7.8e-231
Identity = 401/740 (54.19%), Postives = 513/740 (69.32%), Query Frame = 1

Query: 1   MDRSAMPDSFTNHFEWYSNVLTNVVLDLQREGNGGGGEERIIYGYHNVFHGVAARLSEEE 60
           M +S MP SF  H  WY + L ++                ++Y Y N  HG + RL++EE
Sbjct: 36  MAKSQMPSSFDLHSNWYDSSLRSI-----------SDSAELLYTYENAIHGFSTRLTQEE 95

Query: 61  VEKLEEEDGVVAIFPEMKYELHTTRSPRFLGLEPADSNSAWSQQIADHDVVVGVLDTGIW 120
            + L  + GV+++ PE +YELHTTR+P FLGL+   ++  + +  +  DVVVGVLDTG+W
Sbjct: 96  ADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTAD-LFPEAGSYSDVVVGVLDTGVW 155

Query: 121 PESDSFDDAGMSPVPAHWKGECETGRGFTKQNCNRKIVGARVFYRGYQAATGKFNEQLEY 180
           PES S+ D G  P+P+ WKG CE G  FT   CNRK++GAR F RGY++  G  +E  E 
Sbjct: 156 PESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYESTMGPIDESKES 215

Query: 181 KSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGMAPGARIAAYKVCWIGGCFSSDI 240
           +SPRD DGHGTHT++T AGS V GASLLGYA GTARGMAP AR+A YKVCW+GGCFSSDI
Sbjct: 216 RSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCWLGGCFSSDI 275

Query: 241 LSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLT 300
           L+A+D+A+AD VNVLS+SLGGG+S YYRD +++ AF AME G+ VSCSAGN GP   SL+
Sbjct: 276 LAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSLS 335

Query: 301 NVSPWITTVGASTMDRDFPAIVKLGDGRTITGVSLYRGRITIPENKQFPIVYMGSNSSSP 360
           NV+PWITTVGA T+DRDFPA+  LG+G+  TGVSL++G   +P+ K  P +Y G N+S+ 
Sbjct: 336 NVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGE-ALPD-KLLPFIYAG-NASNA 395

Query: 361 DPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKNAGGIGMILSNTAANGEELVAD 420
              +LC+ GTL P  V GKIV+CDRGI+ RVQKG VVK AGG+GMIL+NTAANGEELVAD
Sbjct: 396 TNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILANTAANGEELVAD 455

Query: 421 CHLVPAVAIGEREGKAIKQYALTNRRATATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLE 480
            HL+PA  +GE+ G  I+ Y  T+   TA++  LGT +GVKPSPVVAAFSSRGPN +T  
Sbjct: 456 AHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPN 515

Query: 481 ILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHP 540
           ILKPDL+APGVNILAAWTG  GP+ L +D+RRV+FNI+SGTSMSCPHVSG+AAL+KS HP
Sbjct: 516 ILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHP 575

Query: 541 DWSPSAIKSALMTTAYVHDNTYKPLKDSSAASPSSPYDHGAGHINPRKALDPGLVYEIQP 600
           +WSP+AI+SALMTTAY      KPL D +   PS+P+DHGAGH++P  A +PGL+Y++  
Sbjct: 576 EWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTTATNPGLIYDLTT 635

Query: 601 QDYFDFLCTQDLSPTQLKVFSKYSNRTC-RGLLPNPGDLNYPAISAVFPEKTTVTSLTLH 660
           +DY  FLC  + +  Q++  S+  N TC      +  DLNYP+ +        V +    
Sbjct: 636 EDYLGFLCALNYTSPQIRSVSR-RNYTCDPSKSYSVADLNYPSFAV---NVDGVGAYKYT 695

Query: 661 RTVTNVGPATSSYHAVVSPFKGATVKVEPESLNFTRRYEKVSYRITFV--TKKRQSMPEF 720
           RTVT+VG A +    V S   G  + VEP  LNF    EK SY +TF   + K      F
Sbjct: 696 RTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSNSF 755

Query: 721 GGLIWKDGSHKVRSPIVITW 738
           G + W DG H V SP+ I+W
Sbjct: 756 GSIEWSDGKHVVGSPVAISW 756

BLAST of Cucsa.251250 vs. TAIR10
Match: AT2G05920.1 (AT2G05920.1 Subtilase family protein)

HSP 1 Score: 756.5 bits (1952), Expect = 1.5e-218
Identity = 384/745 (51.54%), Postives = 502/745 (67.38%), Query Frame = 1

Query: 1   MDRSAMPDSFTNHFEWYSNVLTNVVLDLQREGNGGGGEERIIYGYHNVFHGVAARLSEEE 60
           ++ S  P+SF  H +WY++ L +              E  ++Y Y   FHG +A L   E
Sbjct: 34  VNHSDKPESFLTHHDWYTSQLNS--------------ESSLLYTYTTSFHGFSAYLDSTE 93

Query: 61  VEKL-EEEDGVVAIFPEMKYELHTTRSPRFLGLEPADSNSAWSQQIADHDVVVGVLDTGI 120
            + L    + ++ IF +  Y LHTTR+P FLGL             + + V++GVLDTG+
Sbjct: 94  ADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLN--SEFGVHDLGSSSNGVIIGVLDTGV 153

Query: 121 WPESDSFDDAGMSPVPAHWKGECETGRGFTKQNCNRKIVGARVFYRGYQAATGK-FNEQL 180
           WPES SFDD  M  +P+ WKGECE+G  F  + CN+K++GAR F +G+Q A+G  F+ + 
Sbjct: 154 WPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGGFSSKR 213

Query: 181 EYKSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGMAPGARIAAYKVCWIGGCFSS 240
           E  SPRD DGHGTHT+ T AGS V  AS LGYA GTARGMA  AR+A YKVCW  GCF S
Sbjct: 214 ESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWSTGCFGS 273

Query: 241 DILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVS 300
           DIL+A+DRA+ DGV+VLS+SLGGG + YYRD++++ AF AME GVFVSCSAGN GP   S
Sbjct: 274 DILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCSAGNSGPTRAS 333

Query: 301 LTNVSPWITTVGASTMDRDFPAIVKLGDGRTITGVSLYRGRITIPENKQFPIVYMGSNSS 360
           + NV+PW+ TVGA T+DRDFPA   LG+G+ +TGVSLY G       K   +VY   NSS
Sbjct: 334 VANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSG--VGMGTKPLELVYNKGNSS 393

Query: 361 SPDPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKNAGGIGMILSNTAANGEELV 420
           S   S+LCL G+LD   V GKIV+CDRG++ RV+KG VV++AGG+GMI++NTAA+GEELV
Sbjct: 394 S---SNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAASGEELV 453

Query: 421 ADCHLVPAVAIGEREGKAIKQYALTNRRATATLGFLGTRLGVKPSPVVAAFSSRGPNFLT 480
           AD HL+PA+A+G++ G  +++Y  ++ + TA L F GT L VKPSPVVAAFSSRGPN +T
Sbjct: 454 ADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPVVAAFSSRGPNTVT 513

Query: 481 LEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSGTSMSCPHVSGVAALIKSK 540
            EILKPD++ PGVNILA W+   GP+ L  D+RR +FNI+SGTSMSCPH+SG+A L+K+ 
Sbjct: 514 PEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGLLKAA 573

Query: 541 HPDWSPSAIKSALMTTAYVHDNTYKPLKDSSAASPSSPYDHGAGHINPRKALDPGLVYEI 600
           HP+WSPSAIKSALMTTAYV DNT  PL D++  S S+PY HG+GH++P+KAL PGLVY+I
Sbjct: 574 HPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLVYDI 633

Query: 601 QPQDYFDFLCTQDLSPTQLKVFSKYSNRTCRGLLPNPGDLNYPAISAVFPEKTTVTSLTL 660
             ++Y  FLC+ D +   +    K  +  C     +PG LNYP+ S +F  K  V     
Sbjct: 634 STEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNYPSFSVLFGGKRVV---RY 693

Query: 661 HRTVTNVGPATSSYHAVVSPFKGATVKVEPESLNFTRRYEKVSYRITFVTKKRQSM---P 720
            R VTNVG A+S Y   V+      + V+P  L+F    EK  Y +TFV+KK  SM    
Sbjct: 694 TREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMTNKA 753

Query: 721 EFGGLIWKDGSHKVRSPIVITWLSF 741
           EFG + W +  H+VRSP+  +W  F
Sbjct: 754 EFGSITWSNPQHEVRSPVAFSWNRF 754

BLAST of Cucsa.251250 vs. TAIR10
Match: AT3G14240.1 (AT3G14240.1 Subtilase family protein)

HSP 1 Score: 747.3 bits (1928), Expect = 9.2e-216
Identity = 376/752 (50.00%), Postives = 511/752 (67.95%), Query Frame = 1

Query: 1   MDRSAMPDSFTNHFEWYSNVLTNVVLDLQREGNGGGGEERIIYGYHNVFHGVAARLSEEE 60
           +D  A P  F  HF WY++ L ++                II+ Y  VFHG +ARL+ ++
Sbjct: 32  VDHEAKPSIFPTHFHWYTSSLASLT----------SSPPSIIHTYDTVFHGFSARLTSQD 91

Query: 61  VEKLEEEDGVVAIFPEMKYELHTTRSPRFLGLEPADSNSAWSQQIADHDVVVGVLDTGIW 120
             +L +   V+++ PE    LHTTRSP FLGL   D      +     D+V+GV+DTG+W
Sbjct: 92  ASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEESDFGSDLVIGVIDTGVW 151

Query: 121 PESDSFDDAGMSPVPAHWKGECETGRGFTKQNCNRKIVGARVFYRGYQAATGKFNEQLEY 180
           PE  SFDD G+ PVP  WKG+C   + F +  CNRK+VGAR F  GY+A  GK NE  E+
Sbjct: 152 PERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCGGYEATNGKMNETTEF 211

Query: 181 KSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGMAPGARIAAYKVCWIGGCFSSDI 240
           +SPRD DGHGTHTA+  AG  V  AS LGYA+G A GMAP AR+AAYKVCW  GC+ SDI
Sbjct: 212 RSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVCWNSGCYDSDI 271

Query: 241 LSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLT 300
           L+A D AVADGV+V+S+S+GG V  YY D++++ AFGA++ G+FVS SAGNGGP  +++T
Sbjct: 272 LAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFVSASAGNGGPGALTVT 331

Query: 301 NVSPWITTVGASTMDRDFPAIVKLGDGRTITGVSLYRGRITIPENKQFPIVYMGSNSSSP 360
           NV+PW+TTVGA T+DRDFPA VKLG+G+ I+GVS+Y G    P  + +P+VY GS     
Sbjct: 332 NVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDP-GRMYPLVYGGSLLGGD 391

Query: 361 D-PSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKNAGGIGMILSNTAANGEELVA 420
              SSLCLEG+LDP+ V GKIV+CDRGI+ R  KG +V+  GG+GMI++N   +GE LVA
Sbjct: 392 GYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKNGGLGMIIANGVFDGEGLVA 451

Query: 421 DCHLVPAVAIGEREGKAIKQY------ALTNRRATATLGFLGTRLGVKPSPVVAAFSSRG 480
           DCH++PA ++G   G  I++Y      + +++  TAT+ F GTRLG++P+PVVA+FS+RG
Sbjct: 452 DCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLGIRPAPVVASFSARG 511

Query: 481 PNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSGTSMSCPHVSGVAA 540
           PN  T EILKPD++APG+NILAAW  + GPS +T+D RR +FNILSGTSM+CPHVSG+AA
Sbjct: 512 PNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILSGTSMACPHVSGLAA 571

Query: 541 LIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSSAASPSSPYDHGAGHINPRKALDPG 600
           L+K+ HPDWSP+AI+SAL+TTAY  DN+ +P+ D S  + SS  D+G+GH++P KA+DPG
Sbjct: 572 LLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNTSSVMDYGSGHVHPTKAMDPG 631

Query: 601 LVYEIQPQDYFDFLCTQDLSPTQLKVFSKYSNRTCRGL--LPNPGDLNYPAISAVFPEKT 660
           LVY+I   DY +FLC  + + T +   ++     C G     + G+LNYP+ S VF ++ 
Sbjct: 632 LVYDITSYDYINFLCNSNYTRTNIVTITR-RQADCDGARRAGHVGNLNYPSFSVVF-QQY 691

Query: 661 TVTSLTLH--RTVTNVGPATSSYHAVVSPFKGATVKVEPESLNFTRRYEKVSYRITFVTK 720
             + ++ H  RTVTNVG + S Y   + P +G TV VEPE L+F R  +K+S+ +   T 
Sbjct: 692 GESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFRRVGQKLSFVVRVKTT 751

Query: 721 KRQSMP-----EFGGLIWKDGSHKVRSPIVIT 737
           + +  P     E G ++W DG   V SP+V+T
Sbjct: 752 EVKLSPGATNVETGHIVWSDGKRNVTSPLVVT 770

BLAST of Cucsa.251250 vs. TAIR10
Match: AT4G34980.1 (AT4G34980.1 subtilisin-like serine protease 2)

HSP 1 Score: 744.6 bits (1921), Expect = 6.0e-215
Identity = 382/749 (51.00%), Postives = 504/749 (67.29%), Query Frame = 1

Query: 1   MDRSAMPDSFTNHFEWYSNVLTNVVLDLQREGNGGGGEERIIYGYHNVFHGVAARLSEEE 60
           +D  +MP  F  H+ WYS                   E RI++ YH VFHG +A ++ +E
Sbjct: 32  IDGGSMPSIFPTHYHWYSTEFAE--------------ESRIVHVYHTVFHGFSAVVTPDE 91

Query: 61  VEKLEEEDGVVAIFPEMKYELHTTRSPRFLGLEPADSNSAWSQQIADHDVVVGVLDTGIW 120
            + L     V+A+F + + ELHTTRSP+FLGL+  +    WS+     DV++GV DTGIW
Sbjct: 92  ADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQ--NQKGLWSESDYGSDVIIGVFDTGIW 151

Query: 121 PESDSFDDAGMSPVPAHWKGECETGRGFTKQNCNRKIVGARVFYRGYQAAT-GKFNEQLE 180
           PE  SF D  + P+P  W+G CE+G  F+ +NCNRKI+GAR F +G QAA  G  N+ +E
Sbjct: 152 PERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKIIGARFFAKGQQAAVIGGINKTVE 211

Query: 181 YKSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGMAPGARIAAYKVCWI-GGCFSS 240
           + SPRD DGHGTHT++T AG     AS+ GYA G A+G+AP ARIAAYKVCW   GC  S
Sbjct: 212 FLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAKGVAPKARIAAYKVCWKDSGCLDS 271

Query: 241 DILSAVDRAVADGVNVLSISLGGG---VSSYYRDSLSVAAFGAMEMGVFVSCSAGNGGPD 300
           DIL+A D AV DGV+V+SIS+GGG    S YY D +++ ++GA   G+FVS SAGN GP+
Sbjct: 272 DILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAIGSYGAASKGIFVSSSAGNEGPN 331

Query: 301 PVSLTNVSPWITTVGASTMDRDFPAIVKLGDGRTITGVSLYRGRITIPEN-KQFPIVYMG 360
            +S+TN++PW+TTVGAST+DR+FPA   LGDG  + GVSLY G   +P N + FP+VY G
Sbjct: 332 GMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRGVSLYAG---VPLNGRMFPVVYPG 391

Query: 361 SNSSSPDPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKNAGGIGMILSNTAANG 420
            +  S   +SLC+E TLDP  V GKIVICDRG SPRV KG+VVK AGG+GMIL+N A+NG
Sbjct: 392 KSGMSS--ASLCMENTLDPKQVRGKIVICDRGSSPRVAKGLVVKKAGGVGMILANGASNG 451

Query: 421 EELVADCHLVPAVAIGEREGKAIKQYALTNRRATATLGFLGTRLGVKPSPVVAAFSSRGP 480
           E LV D HL+PA A+G  EG  IK YA ++    A++ F GT +G+KP+PV+A+FS RGP
Sbjct: 452 EGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASIDFRGTIVGIKPAPVIASFSGRGP 511

Query: 481 NFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSGTSMSCPHVSGVAAL 540
           N L+ EILKPDL+APGVNILAAWT   GP+ L +D R+ +FNILSGTSM+CPHVSG AAL
Sbjct: 512 NGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNILSGTSMACPHVSGAAAL 571

Query: 541 IKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSSAASPSSPYDHGAGHINPRKALDPGL 600
           +KS HPDWSP+ I+SA+MTT  + DN+ + L D S    ++PYD+G+GH+N  +A++PGL
Sbjct: 572 LKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDESTGKSATPYDYGSGHLNLGRAMNPGL 631

Query: 601 VYEIQPQDYFDFLCTQDLSPTQLKVFSKYSNRTCRGLLPNPGDLNYPAISAVFP-EKTTV 660
           VY+I   DY  FLC+    P  ++V ++   R      P+PG+LNYP+I+AVFP  +  +
Sbjct: 632 VYDITNDDYITFLCSIGYGPKTIQVITRTPVRCPTTRKPSPGNLNYPSITAVFPTNRRGL 691

Query: 661 TSLTLHRTVTNVGPATSSYHAVVSPFKGATVKVEPESLNFTRRYEKVSYRITFVTKKR-- 720
            S T+ RT TNVG A + Y A +   +G TV V+P  L FT   ++ SY +T     R  
Sbjct: 692 VSKTVIRTATNVGQAEAVYRARIESPRGVTVTVKPPRLVFTSAVKRRSYAVTVTVNTRNV 751

Query: 721 ---QSMPEFGGLIWKD-GSHKVRSPIVIT 737
              ++   FG + W D G H VRSPIV+T
Sbjct: 752 VLGETGAVFGSVTWFDGGKHVVRSPIVVT 759

BLAST of Cucsa.251250 vs. NCBI nr
Match: gi|778679387|ref|XP_004148149.2| (PREDICTED: subtilisin-like protease SBT1.7 [Cucumis sativus])

HSP 1 Score: 1501.9 bits (3887), Expect = 0.0e+00
Identity = 741/741 (100.00%), Postives = 741/741 (100.00%), Query Frame = 1

Query: 1   MDRSAMPDSFTNHFEWYSNVLTNVVLDLQREGNGGGGEERIIYGYHNVFHGVAARLSEEE 60
           MDRSAMPDSFTNHFEWYSNVLTNVVLDLQREGNGGGGEERIIYGYHNVFHGVAARLSEEE
Sbjct: 67  MDRSAMPDSFTNHFEWYSNVLTNVVLDLQREGNGGGGEERIIYGYHNVFHGVAARLSEEE 126

Query: 61  VEKLEEEDGVVAIFPEMKYELHTTRSPRFLGLEPADSNSAWSQQIADHDVVVGVLDTGIW 120
           VEKLEEEDGVVAIFPEMKYELHTTRSPRFLGLEPADSNSAWSQQIADHDVVVGVLDTGIW
Sbjct: 127 VEKLEEEDGVVAIFPEMKYELHTTRSPRFLGLEPADSNSAWSQQIADHDVVVGVLDTGIW 186

Query: 121 PESDSFDDAGMSPVPAHWKGECETGRGFTKQNCNRKIVGARVFYRGYQAATGKFNEQLEY 180
           PESDSFDDAGMSPVPAHWKGECETGRGFTKQNCNRKIVGARVFYRGYQAATGKFNEQLEY
Sbjct: 187 PESDSFDDAGMSPVPAHWKGECETGRGFTKQNCNRKIVGARVFYRGYQAATGKFNEQLEY 246

Query: 181 KSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGMAPGARIAAYKVCWIGGCFSSDI 240
           KSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGMAPGARIAAYKVCWIGGCFSSDI
Sbjct: 247 KSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGMAPGARIAAYKVCWIGGCFSSDI 306

Query: 241 LSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLT 300
           LSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLT
Sbjct: 307 LSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLT 366

Query: 301 NVSPWITTVGASTMDRDFPAIVKLGDGRTITGVSLYRGRITIPENKQFPIVYMGSNSSSP 360
           NVSPWITTVGASTMDRDFPAIVKLGDGRTITGVSLYRGRITIPENKQFPIVYMGSNSSSP
Sbjct: 367 NVSPWITTVGASTMDRDFPAIVKLGDGRTITGVSLYRGRITIPENKQFPIVYMGSNSSSP 426

Query: 361 DPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKNAGGIGMILSNTAANGEELVAD 420
           DPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKNAGGIGMILSNTAANGEELVAD
Sbjct: 427 DPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKNAGGIGMILSNTAANGEELVAD 486

Query: 421 CHLVPAVAIGEREGKAIKQYALTNRRATATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLE 480
           CHLVPAVAIGEREGKAIKQYALTNRRATATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLE
Sbjct: 487 CHLVPAVAIGEREGKAIKQYALTNRRATATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLE 546

Query: 481 ILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHP 540
           ILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHP
Sbjct: 547 ILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHP 606

Query: 541 DWSPSAIKSALMTTAYVHDNTYKPLKDSSAASPSSPYDHGAGHINPRKALDPGLVYEIQP 600
           DWSPSAIKSALMTTAYVHDNTYKPLKDSSAASPSSPYDHGAGHINPRKALDPGLVYEIQP
Sbjct: 607 DWSPSAIKSALMTTAYVHDNTYKPLKDSSAASPSSPYDHGAGHINPRKALDPGLVYEIQP 666

Query: 601 QDYFDFLCTQDLSPTQLKVFSKYSNRTCRGLLPNPGDLNYPAISAVFPEKTTVTSLTLHR 660
           QDYFDFLCTQDLSPTQLKVFSKYSNRTCRGLLPNPGDLNYPAISAVFPEKTTVTSLTLHR
Sbjct: 667 QDYFDFLCTQDLSPTQLKVFSKYSNRTCRGLLPNPGDLNYPAISAVFPEKTTVTSLTLHR 726

Query: 661 TVTNVGPATSSYHAVVSPFKGATVKVEPESLNFTRRYEKVSYRITFVTKKRQSMPEFGGL 720
           TVTNVGPATSSYHAVVSPFKGATVKVEPESLNFTRRYEKVSYRITFVTKKRQSMPEFGGL
Sbjct: 727 TVTNVGPATSSYHAVVSPFKGATVKVEPESLNFTRRYEKVSYRITFVTKKRQSMPEFGGL 786

Query: 721 IWKDGSHKVRSPIVITWLSFV 742
           IWKDGSHKVRSPIVITWLSFV
Sbjct: 787 IWKDGSHKVRSPIVITWLSFV 807

BLAST of Cucsa.251250 vs. NCBI nr
Match: gi|659077296|ref|XP_008439131.1| (PREDICTED: subtilisin-like protease [Cucumis melo])

HSP 1 Score: 1460.7 bits (3780), Expect = 0.0e+00
Identity = 716/741 (96.63%), Postives = 731/741 (98.65%), Query Frame = 1

Query: 1   MDRSAMPDSFTNHFEWYSNVLTNVVLDLQREGNGGGGEERIIYGYHNVFHGVAARLSEEE 60
           MDRSAMP SFTNHFEWYSNVL+NVV+DL+REGNGGGGEERIIY Y NVFHGVAARLSEEE
Sbjct: 67  MDRSAMPASFTNHFEWYSNVLSNVVVDLEREGNGGGGEERIIYSYQNVFHGVAARLSEEE 126

Query: 61  VEKLEEEDGVVAIFPEMKYELHTTRSPRFLGLEPADSNSAWSQQIADHDVVVGVLDTGIW 120
           VEKLEEEDGVVAIFPEMKYELHTTRSPRFLGLEPADSNSAWSQQIADHDVVVGVLDTGIW
Sbjct: 127 VEKLEEEDGVVAIFPEMKYELHTTRSPRFLGLEPADSNSAWSQQIADHDVVVGVLDTGIW 186

Query: 121 PESDSFDDAGMSPVPAHWKGECETGRGFTKQNCNRKIVGARVFYRGYQAATGKFNEQLEY 180
           PESDSFDDAGMSPVPAHWKGECETGRGFTKQNCNRKIVGARVFY GYQAATGKFNEQLEY
Sbjct: 187 PESDSFDDAGMSPVPAHWKGECETGRGFTKQNCNRKIVGARVFYHGYQAATGKFNEQLEY 246

Query: 181 KSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGMAPGARIAAYKVCWIGGCFSSDI 240
           KSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGMAPGARIAAYKVCWIGGCFSSDI
Sbjct: 247 KSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGMAPGARIAAYKVCWIGGCFSSDI 306

Query: 241 LSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLT 300
           LSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLT
Sbjct: 307 LSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLT 366

Query: 301 NVSPWITTVGASTMDRDFPAIVKLGDGRTITGVSLYRGRITIPENKQFPIVYMGSNSSSP 360
           NVSPWITTVGASTMDRDFPAIVKLGDGRT++G SLYRGRITIPENKQFPIVYMGSNSSSP
Sbjct: 367 NVSPWITTVGASTMDRDFPAIVKLGDGRTMSGASLYRGRITIPENKQFPIVYMGSNSSSP 426

Query: 361 DPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKNAGGIGMILSNTAANGEELVAD 420
           DPSSLCLEGTLDPH VAGKIVICDRGISPRVQKGVVVKNAGGIGMILSNT ANGEELVAD
Sbjct: 427 DPSSLCLEGTLDPHVVAGKIVICDRGISPRVQKGVVVKNAGGIGMILSNTVANGEELVAD 486

Query: 421 CHLVPAVAIGEREGKAIKQYALTNRRATATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLE 480
           CHLVPAVA+GE+EGKAIKQYALTNR+ATATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLE
Sbjct: 487 CHLVPAVAVGEKEGKAIKQYALTNRKATATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLE 546

Query: 481 ILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHP 540
           ILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHP
Sbjct: 547 ILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHP 606

Query: 541 DWSPSAIKSALMTTAYVHDNTYKPLKDSSAASPSSPYDHGAGHINPRKALDPGLVYEIQP 600
           DWSPSAIKSALMTTAY+HDNTYKPL+DSSAASPSSPYDHGAGHINPRKALDPGLVYEIQP
Sbjct: 607 DWSPSAIKSALMTTAYIHDNTYKPLQDSSAASPSSPYDHGAGHINPRKALDPGLVYEIQP 666

Query: 601 QDYFDFLCTQDLSPTQLKVFSKYSNRTCRGLLPNPGDLNYPAISAVFPEKTTVTSLTLHR 660
           QDYFDFLCTQDL+PTQLKVFSKYSNRTCR LLPNPGDLNYPAISAVFPEKT+VTSLTLHR
Sbjct: 667 QDYFDFLCTQDLTPTQLKVFSKYSNRTCRRLLPNPGDLNYPAISAVFPEKTSVTSLTLHR 726

Query: 661 TVTNVGPATSSYHAVVSPFKGATVKVEPESLNFTRRYEKVSYRITFVTKKRQSMPEFGGL 720
           TVTNVGPATSSYHAVV+PFKGA VKVEPESLNFTRRYEK+SYRITFVTKKR SMPEFGGL
Sbjct: 727 TVTNVGPATSSYHAVVTPFKGAAVKVEPESLNFTRRYEKLSYRITFVTKKRLSMPEFGGL 786

Query: 721 IWKDGSHKVRSPIVITWLSFV 742
           IWKDGSHKVRSPIV+TWLSFV
Sbjct: 787 IWKDGSHKVRSPIVMTWLSFV 807

BLAST of Cucsa.251250 vs. NCBI nr
Match: gi|596038840|ref|XP_007219861.1| (hypothetical protein PRUPE_ppa1027166mg [Prunus persica])

HSP 1 Score: 1229.5 bits (3180), Expect = 0.0e+00
Identity = 583/739 (78.89%), Postives = 666/739 (90.12%), Query Frame = 1

Query: 1   MDRSAMPDSFTNHFEWYSNVLTNVVLDLQREGNGGGGEERIIYGYHNVFHGVAARLSEEE 60
           MD+SA P+SFTNH +WYS+ + ++V   + E +GG  +ER+IY Y N FHGVAARLSEEE
Sbjct: 39  MDKSAKPESFTNHLDWYSSKVNSIVFKPENEEDGGHDQERVIYTYQNAFHGVAARLSEEE 98

Query: 61  VEKLEEEDGVVAIFPEMKYELHTTRSPRFLGLEPADSNSA-WSQQIADHDVVVGVLDTGI 120
            E+L+E+DGV+AIFP+ KY+LHTTRSP FLGLEP DS +  WSQ++ DHDV+VGVLDTG+
Sbjct: 99  AERLQEQDGVLAIFPDTKYQLHTTRSPLFLGLEPHDSTTTVWSQRVTDHDVIVGVLDTGV 158

Query: 121 WPESDSFDDAGMSPVPAHWKGECETGRGFTKQNCNRKIVGARVFYRGYQAATGKFNEQLE 180
           WPES SF+D GMSPVPA+WKG CETGRGF+K NCN+KIVGAR+FY GY+AATGK NEQ E
Sbjct: 159 WPESQSFNDTGMSPVPAYWKGACETGRGFSKHNCNKKIVGARIFYHGYEAATGKINEQTE 218

Query: 181 YKSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGMAPGARIAAYKVCWIGGCFSSD 240
           +KSPRDQDGHGTHTAATVAGSPV GA+LLGYA+GTARGMAPGARIAAYKVCW+GGCFSSD
Sbjct: 219 FKSPRDQDGHGTHTAATVAGSPVRGANLLGYAHGTARGMAPGARIAAYKVCWVGGCFSSD 278

Query: 241 ILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSL 300
           ILSAVD+AVADGVNVLSISLGGGVS+YYRDSLS+AAFGAMEMGVFVSCSAGNGGPDPVSL
Sbjct: 279 ILSAVDKAVADGVNVLSISLGGGVSAYYRDSLSIAAFGAMEMGVFVSCSAGNGGPDPVSL 338

Query: 301 TNVSPWITTVGASTMDRDFPAIVKLGDGRTITGVSLYRGRITIPENKQFPIVYMGSNSSS 360
           TNVSPWITTVGASTMDRDFP+ VKLG+GRT+TGVSLY+GR+ +  NKQ+P+VYMG NS+S
Sbjct: 339 TNVSPWITTVGASTMDRDFPSSVKLGNGRTVTGVSLYKGRMMLSTNKQYPVVYMGDNSTS 398

Query: 361 PDPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKNAGGIGMILSNTAANGEELVA 420
           PDPSSLCLEGTLD   VAGKIVICDRGISPRVQKG VVK+AGG+GMIL+NTAANGEELVA
Sbjct: 399 PDPSSLCLEGTLDRRVVAGKIVICDRGISPRVQKGQVVKDAGGVGMILANTAANGEELVA 458

Query: 421 DCHLVPAVAIGEREGKAIKQYALTNRRATATLGFLGTRLGVKPSPVVAAFSSRGPNFLTL 480
           DCHLVPAVA+GE E KAIK YALT+ RATATL FLGTR GV+PSPVVAAFSSRGPNF++L
Sbjct: 459 DCHLVPAVAVGETEAKAIKHYALTSPRATATLAFLGTRTGVRPSPVVAAFSSRGPNFVSL 518

Query: 481 EILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSGTSMSCPHVSGVAALIKSKH 540
           EILKPD+VAPGVNILAAWTG  GPSSL TD RRVKFNILSGTSMSCPHVSG+AAL+K++H
Sbjct: 519 EILKPDVVAPGVNILAAWTGALGPSSLPTDHRRVKFNILSGTSMSCPHVSGIAALLKARH 578

Query: 541 PDWSPSAIKSALMTTAYVHDNTYKPLKDSSAASPSSPYDHGAGHINPRKALDPGLVYEIQ 600
           P+WSP+AIKSALMTTAYVHDNT+KPL+D+SAA  S+PYDHGAGHINPRKALDPGLVY+I+
Sbjct: 579 PEWSPAAIKSALMTTAYVHDNTHKPLQDASAAEASTPYDHGAGHINPRKALDPGLVYDIE 638

Query: 601 PQDYFDFLCTQDLSPTQLKVFSKYSNRTCRGLLPNPGDLNYPAISAVFPEKTTVTSLTLH 660
            QDY +FLCTQ L+P QLKVF+KYSNR+C+  L +PGDLNYPAIS VFPE+T V+ LTLH
Sbjct: 639 AQDYLEFLCTQRLTPMQLKVFTKYSNRSCKHALASPGDLNYPAISVVFPERTNVSLLTLH 698

Query: 661 RTVTNVGPATSSYHAVVSPFKGATVKVEPESLNFTRRYEKVSYRITFVTKKRQSMPEFGG 720
           RTVTNVGP  S+YHA+VSPFKGA VKVEP +L FTR  +K+SY+ITF TK RQ+ PEFGG
Sbjct: 699 RTVTNVGPPVSNYHAIVSPFKGAYVKVEPRTLKFTRANQKLSYKITFTTKSRQATPEFGG 758

Query: 721 LIWKDGSHKVRSPIVITWL 739
           L+WKDG H+VRSPIV+ WL
Sbjct: 759 LVWKDGVHRVRSPIVVVWL 777

BLAST of Cucsa.251250 vs. NCBI nr
Match: gi|645257258|ref|XP_008234331.1| (PREDICTED: subtilisin-like protease [Prunus mume])

HSP 1 Score: 1228.8 bits (3178), Expect = 0.0e+00
Identity = 583/739 (78.89%), Postives = 666/739 (90.12%), Query Frame = 1

Query: 1   MDRSAMPDSFTNHFEWYSNVLTNVVLDLQREGNGGGGEERIIYGYHNVFHGVAARLSEEE 60
           MD+SA P+SFTNH +WYS+ + ++V   + E +GG  +ER+IY Y N FHGVAARLSEEE
Sbjct: 100 MDKSAKPESFTNHLDWYSSKVNSIVFKPENEEDGGHNQERVIYAYQNAFHGVAARLSEEE 159

Query: 61  VEKLEEEDGVVAIFPEMKYELHTTRSPRFLGLEPADSNS-AWSQQIADHDVVVGVLDTGI 120
            E+L+E+DGV+AIFP+ KY+LHTTRSP FLGLEP DS +  WSQ++ DHDV+VGVLDTG+
Sbjct: 160 AERLQEQDGVLAIFPDTKYQLHTTRSPLFLGLEPHDSTTNVWSQRVTDHDVIVGVLDTGV 219

Query: 121 WPESDSFDDAGMSPVPAHWKGECETGRGFTKQNCNRKIVGARVFYRGYQAATGKFNEQLE 180
           WPES SF+D GMSPVPA WKG CETGRGF+K NCN+KIVGAR+FY+GY+AATGK NEQ E
Sbjct: 220 WPESQSFNDTGMSPVPARWKGACETGRGFSKHNCNKKIVGARIFYQGYEAATGKINEQTE 279

Query: 181 YKSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGMAPGARIAAYKVCWIGGCFSSD 240
           +KSPRDQDGHGTHTAATVAGSPV GA+LLGYA+GTARGMAPGARIAAYKVCW+GGCFSSD
Sbjct: 280 FKSPRDQDGHGTHTAATVAGSPVRGANLLGYAHGTARGMAPGARIAAYKVCWVGGCFSSD 339

Query: 241 ILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSL 300
           ILSAVD+AVADGVNVLSISLGGGVS+YYRDSLS+AAFGAMEMGVFVSCSAGNGGPDPVSL
Sbjct: 340 ILSAVDKAVADGVNVLSISLGGGVSAYYRDSLSIAAFGAMEMGVFVSCSAGNGGPDPVSL 399

Query: 301 TNVSPWITTVGASTMDRDFPAIVKLGDGRTITGVSLYRGRITIPENKQFPIVYMGSNSSS 360
           TNVSPWITTVGASTMDRDFP+ VKLG+GRT+TGVSLY+G + +  NKQ+P+VYMG+NS+S
Sbjct: 400 TNVSPWITTVGASTMDRDFPSTVKLGNGRTVTGVSLYKGTMMLSTNKQYPVVYMGNNSTS 459

Query: 361 PDPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKNAGGIGMILSNTAANGEELVA 420
           PDPSSLCLEGTLD   VAGKIVICDRGISPRVQKG VVK+AGG+GMIL+NTAANGEELVA
Sbjct: 460 PDPSSLCLEGTLDRRVVAGKIVICDRGISPRVQKGQVVKDAGGVGMILANTAANGEELVA 519

Query: 421 DCHLVPAVAIGEREGKAIKQYALTNRRATATLGFLGTRLGVKPSPVVAAFSSRGPNFLTL 480
           DCHLVPAVA+GE E KAIK YALT+ RATATL FLGTR GV+PSPVVAAFSSRGPNF++L
Sbjct: 520 DCHLVPAVAVGETEAKAIKHYALTSPRATATLAFLGTRTGVRPSPVVAAFSSRGPNFVSL 579

Query: 481 EILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSGTSMSCPHVSGVAALIKSKH 540
           EILKPD+VAPGVNILAAWTG  GPSSL TD RRVKFNILSGTSMSCPHVSG+AAL+K++H
Sbjct: 580 EILKPDVVAPGVNILAAWTGALGPSSLPTDHRRVKFNILSGTSMSCPHVSGIAALLKARH 639

Query: 541 PDWSPSAIKSALMTTAYVHDNTYKPLKDSSAASPSSPYDHGAGHINPRKALDPGLVYEIQ 600
           P+WSP+AIKSALMTTAYVHDNT+KPL+D+SAA  S+PYDHGAGHINPRKALDPGLVY+I+
Sbjct: 640 PEWSPAAIKSALMTTAYVHDNTHKPLQDASAAEASTPYDHGAGHINPRKALDPGLVYDIE 699

Query: 601 PQDYFDFLCTQDLSPTQLKVFSKYSNRTCRGLLPNPGDLNYPAISAVFPEKTTVTSLTLH 660
            QDY +FLCTQ L+P QLKVF+KYSNR+C+  L +PGDLNYPAIS VFPE+T V+ LTLH
Sbjct: 700 AQDYLEFLCTQRLTPMQLKVFTKYSNRSCKHSLASPGDLNYPAISVVFPERTNVSLLTLH 759

Query: 661 RTVTNVGPATSSYHAVVSPFKGATVKVEPESLNFTRRYEKVSYRITFVTKKRQSMPEFGG 720
           RTVTNVGP  S+YHA+VSPFKGA VKVEP +L FTR  +K+SY+ITF TK RQ+ PEFGG
Sbjct: 760 RTVTNVGPPVSNYHAIVSPFKGAYVKVEPRTLKFTRANQKLSYKITFTTKSRQATPEFGG 819

Query: 721 LIWKDGSHKVRSPIVITWL 739
           L+WKDG H+VRSPIVI WL
Sbjct: 820 LVWKDGVHRVRSPIVIVWL 838

BLAST of Cucsa.251250 vs. NCBI nr
Match: gi|590672084|ref|XP_007038510.1| (Subtilase 1.3 [Theobroma cacao])

HSP 1 Score: 1226.8 bits (3173), Expect = 0.0e+00
Identity = 593/740 (80.14%), Postives = 667/740 (90.14%), Query Frame = 1

Query: 1   MDRSAMPDSFTNHFEWYSNVLTNVVL-DLQREGNGGGGEERIIYGYHNVFHGVAARLSEE 60
           MD+SAMP SF++H EWYS+ + +V++ + Q EG+G G  ERIIY Y N FHGVAA+L+E+
Sbjct: 38  MDKSAMPASFSSHLEWYSSKVKSVIMSNTQSEGDGDG--ERIIYSYQNAFHGVAAQLTED 97

Query: 61  EVEKLEEEDGVVAIFPEMKYELHTTRSPRFLGLEPADSNSAWSQQIADHDVVVGVLDTGI 120
           E E+LEEEDGVVAI PEMKY+LHTTRSP FLGLEP +S S WSQ++ DHDV+VGVLDTGI
Sbjct: 98  EAERLEEEDGVVAILPEMKYQLHTTRSPMFLGLEPEESTSIWSQKLTDHDVIVGVLDTGI 157

Query: 121 WPESDSFDDAGMSPVPAHWKGECETGRGFTKQNCNRKIVGARVFYRGYQAATGKFNEQLE 180
           WPES+SF+D G++PVPAHWKG CETGRGF K +CNRKIVGARVFYRGY+AATGK NE+ E
Sbjct: 158 WPESESFNDTGLAPVPAHWKGACETGRGFEKHHCNRKIVGARVFYRGYEAATGKINEKNE 217

Query: 181 YKSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGMAPGARIAAYKVCWIGGCFSSD 240
           YKSPRDQDGHGTHTAATVAGSPV GA+LLGYAYGTARGMAPGARIAAYKVCW GGCFSSD
Sbjct: 218 YKSPRDQDGHGTHTAATVAGSPVRGANLLGYAYGTARGMAPGARIAAYKVCWTGGCFSSD 277

Query: 241 ILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSL 300
           ILSAVDRAVADGV+VLSISLGGGVSSYYRDSL++A FGAMEMGVFVSCSAGNGGPDPVSL
Sbjct: 278 ILSAVDRAVADGVSVLSISLGGGVSSYYRDSLAIATFGAMEMGVFVSCSAGNGGPDPVSL 337

Query: 301 TNVSPWITTVGASTMDRDFPAIVKLGDGRTITGVSLYRGRITIPENKQFPIVYMGSNSSS 360
           TNVSPWITTVGASTMDRDFPA VKLG GRT+TGVSLY+G+  +  NKQ+PIVYMGSNSSS
Sbjct: 338 TNVSPWITTVGASTMDRDFPADVKLGTGRTLTGVSLYKGQRFLSPNKQYPIVYMGSNSSS 397

Query: 361 PDPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKNAGGIGMILSNTAANGEELVA 420
           PDPSSLCLEGTLDPH V+GKIVICDRGISPRVQKG VVK+AGGIGMIL+NTAANGEELVA
Sbjct: 398 PDPSSLCLEGTLDPHIVSGKIVICDRGISPRVQKGQVVKDAGGIGMILTNTAANGEELVA 457

Query: 421 DCHLVPAVAIGEREGKAIKQYALTNRRATATLGFLGTRLGVKPSPVVAAFSSRGPNFLTL 480
           DCHL+PA+A+GE EGKAIK YALT+R+ATATL FLGTRLG++PSPVVAAFSSRGPNFLT 
Sbjct: 458 DCHLLPALAVGEMEGKAIKHYALTSRKATATLAFLGTRLGIRPSPVVAAFSSRGPNFLTF 517

Query: 481 EILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSGTSMSCPHVSGVAALIKSKH 540
           EILKPD+VAPGVNILAAWTG+ GPSSL TD RRVKFNILSGTSMSCPHVSG+AAL+K++H
Sbjct: 518 EILKPDMVAPGVNILAAWTGELGPSSLQTDHRRVKFNILSGTSMSCPHVSGIAALLKARH 577

Query: 541 PDWSPSAIKSALMTTAYVHDNTYKPLKDSSAASPSSPYDHGAGHINPRKALDPGLVYEIQ 600
           P+WSP+AIKSALMTTAYVHDNT+ PLKD++ A+ S+PYDHGAGHINP KALDPGLVY+I+
Sbjct: 578 PEWSPAAIKSALMTTAYVHDNTHNPLKDAAEAAISTPYDHGAGHINPLKALDPGLVYDIE 637

Query: 601 PQDYFDFLCTQDLSPTQLKVFSKYSNRTCRGLLPNPGDLNYPAISAVFPEKTTVTS-LTL 660
            QDYF+FLCTQ L+  QLKVF KYSNR C   L + GDLNYPAIS VFPE TT  S LTL
Sbjct: 638 AQDYFEFLCTQKLTTMQLKVFGKYSNRFCHHTLASAGDLNYPAISVVFPEDTTAISVLTL 697

Query: 661 HRTVTNVGPATSSYHAVVSPFKGATVKVEPESLNFTRRYEKVSYRITFVTKKRQSMPEFG 720
           HRTVTNVGP  S+YH VVS FKGATVKV+P+SLNFTR+ +K+SY+ITF TK  Q++PEFG
Sbjct: 698 HRTVTNVGPPISNYHVVVSQFKGATVKVDPKSLNFTRKNQKLSYKITFTTKSPQTVPEFG 757

Query: 721 GLIWKDGSHKVRSPIVITWL 739
           GL+WKDG HKVRSPIVITW+
Sbjct: 758 GLVWKDGVHKVRSPIVITWI 775