Cucsa.250620 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.250620 vs. Swiss-Prot
Match: PLP3_ARATH (Patatin-like protein 3 OS=Arabidopsis thaliana GN=PLP3 PE=2 SV=2)

HSP 1 Score: 421.4 bits (1082), Expect = 1.1e-116
Identity = 213/393 (54.20%), Postives = 284/393 (72.26%), Query Frame = 1

Query: 1   MITILSIDGGGIRGIIPSIILAFLESKLQELDGPDVRIADYFDVIAGTSTGGLVTSMLTA 60
           ++TILSIDGGGIRGIIP  ILA+LES+LQELDG + R+ DYFDVI+GTSTGGL+ +MLTA
Sbjct: 34  LVTILSIDGGGIRGIIPGTILAYLESQLQELDGEEARLVDYFDVISGTSTGGLIVAMLTA 93

Query: 61  PDKN-------NRPLYSASDLALFYIEHAPKIFPQRNYFLC----SLVNFFGKVMGPKYN 120
            D++       NRPL+ A ++  FY++H+PKIFPQ     C    ++V   G   GPK+N
Sbjct: 94  QDQSGGHSRNSNRPLFEAKEIVPFYLKHSPKIFPQPRGIFCGWGETIVRLVG---GPKFN 153

Query: 121 GLYLRSLIKGLLGDITLKQTLSQVVIPAFDIKLLQPVIFTTIEAKCSELKNPKLADVCIS 180
           G YL  L++G LGD  L Q+L+ VVIP FDIK LQPVIF++ +A  ++  N KL+D+CIS
Sbjct: 154 GKYLHDLVEGFLGDTKLTQSLTNVVIPCFDIKKLQPVIFSSYQAVNNQAMNAKLSDICIS 213

Query: 181 TSAAPTFLPGYEFQTKDSKGNIRNYEMVDGGVAANNPTLAAMTHVTKEMSILRHRSELLK 240
           TSAAPTF P + F  +DS+G    + ++DGG+AANNPTL A+  VTK+  I++    +  
Sbjct: 214 TSAAPTFFPAHRFTNEDSEGIKHEFNLIDGGIAANNPTLCAIAEVTKQ--IIKKNPVMGD 273

Query: 241 IKPMETERMLVLSLGTGTPKNDEKYSAAKASKWGMLDWVYHGGGTPIVDIFSDASADMVD 300
           I P++  R LV+S+GTG+ +N EKY+A  ASKWG++ WV+  G TPI+D +S+A  DMVD
Sbjct: 274 ISPLDFTRFLVISIGTGSIRNQEKYNAKMASKWGLMCWVFESGSTPILDCYSEAIHDMVD 333

Query: 301 YHISSIFQSDHCHKNYLRIQDDTLSGEVSSVDIATEENLLNLIYVGENLLKKPLSRVNLE 360
           Y  S +FQ+    KNYLRI DD+L G++ SVDI+TE+N+  L+ VGE LLKK +SRVNLE
Sbjct: 334 YQSSVVFQALRSEKNYLRIDDDSLKGDLGSVDISTEKNMEGLVEVGEALLKKRVSRVNLE 393

Query: 361 SGKFEPLDAKGTNEQALAEFAKMLSNERKLRLS 383
           SG ++P+    TNE+AL  FAK+LS ERKLR S
Sbjct: 394 SGHYQPISENVTNEEALKRFAKVLSEERKLRES 421

BLAST of Cucsa.250620 vs. Swiss-Prot
Match: PLP2_ORYSI (Patatin-like protein 2 OS=Oryza sativa subsp. indica GN=PLP2 PE=3 SV=1)

HSP 1 Score: 419.1 bits (1076), Expect = 5.3e-116
Identity = 206/381 (54.07%), Postives = 276/381 (72.44%), Query Frame = 1

Query: 1   MITILSIDGGGIRGIIPSIILAFLESKLQELDGPDVRIADYFDVIAGTSTGGLVTSMLTA 60
           M+T+LSIDGGG+RGIIP+ ILAFLE +LQ+LDGPD RIADYFDV+AGTSTGGL+T+MLTA
Sbjct: 20  MVTVLSIDGGGVRGIIPATILAFLEKELQKLDGPDARIADYFDVVAGTSTGGLLTAMLTA 79

Query: 61  PDKNNRPLYSASDLALFYIEHAPKIFPQRNYFLCSLVNFFGKVMGPKYNGLYLRSLIKGL 120
           P++NNRPL++A +LA FYIEH+P IFPQ+N+ L  +      V GPKY+G YL SL++  
Sbjct: 80  PNENNRPLFAADELAKFYIEHSPSIFPQKNWVLSKIAGTLRMVSGPKYDGKYLHSLLREK 139

Query: 121 LGDITLKQTLSQVVIPAFDIKLLQPVIFTTIEAKCSELKNPKLADVCISTSAAPTFLPGY 180
           LGD  L + L+ VVIP FDI  LQP IF+  E K   LKN  L+D+ ISTSAAPTF P +
Sbjct: 140 LGDTRLDKALTNVVIPTFDIANLQPTIFSKFELKYKPLKNALLSDISISTSAAPTFFPAH 199

Query: 181 EFQTKDSKGNIRNYEMVDGGVAANNPTLAAMTHVTKEMSIL-RHRSELLKIKPMETERML 240
            F+TKD  G  R + +VDGGVAANNPTL AM+ V+K + +  +   +   +KP E  + +
Sbjct: 200 YFETKDDNGQTREFNLVDGGVAANNPTLCAMSQVSKYIILEDKEDCDFFPVKPTEYGKFM 259

Query: 241 VLSLGTGTPKNDEKYSAAKASKWGMLDWVYHGGGTPIVDIFSDASADMVDYHISSIFQSD 300
           V+S+G G+  +D+KY A  A+KWG+ +W+  G   PI+D+F+ ASADMVD H+  +F + 
Sbjct: 260 VISIGCGS-NHDQKYKAKDAAKWGIFNWLIKGSSAPIIDMFTSASADMVDIHLGVLFSAL 319

Query: 301 HCHKNYLRIQDDTLSGEVSSVDIATEENLLNLIYVGENLLKKPLSRVNLESGKFEPLDAK 360
            C KNYLRIQ D L+G   S+D  ++EN+ NL+ +GE LL K +SRV+LE+G +  +  +
Sbjct: 320 QCEKNYLRIQYDQLTGSAGSIDDCSKENMDNLVKIGEMLLDKNVSRVDLETGHYVDVAGE 379

Query: 361 GTNEQALAEFAKMLSNERKLR 381
           GTN   LA+FAK LS+ER+ R
Sbjct: 380 GTNRDQLAKFAKQLSDERRRR 399

BLAST of Cucsa.250620 vs. Swiss-Prot
Match: PLP2_ORYSJ (Patatin-like protein 2 OS=Oryza sativa subsp. japonica GN=PLP2 PE=3 SV=1)

HSP 1 Score: 419.1 bits (1076), Expect = 5.3e-116
Identity = 206/381 (54.07%), Postives = 276/381 (72.44%), Query Frame = 1

Query: 1   MITILSIDGGGIRGIIPSIILAFLESKLQELDGPDVRIADYFDVIAGTSTGGLVTSMLTA 60
           M+T+LSIDGGG+RGIIP+ ILAFLE +LQ+LDGPD RIADYFDV+AGTSTGGL+T+MLTA
Sbjct: 20  MVTVLSIDGGGVRGIIPATILAFLEKELQKLDGPDARIADYFDVVAGTSTGGLLTAMLTA 79

Query: 61  PDKNNRPLYSASDLALFYIEHAPKIFPQRNYFLCSLVNFFGKVMGPKYNGLYLRSLIKGL 120
           P++NNRPL++A +LA FYIEH+P IFPQ+N+ L  +      V GPKY+G YL SL++  
Sbjct: 80  PNENNRPLFAADELAKFYIEHSPSIFPQKNWVLSKIAGTLRMVSGPKYDGKYLHSLLREK 139

Query: 121 LGDITLKQTLSQVVIPAFDIKLLQPVIFTTIEAKCSELKNPKLADVCISTSAAPTFLPGY 180
           LGD  L + L+ VVIP FDI  LQP IF+  E K   LKN  L+D+ ISTSAAPTF P +
Sbjct: 140 LGDTRLDKALTNVVIPTFDIANLQPTIFSKFELKYKPLKNALLSDISISTSAAPTFFPAH 199

Query: 181 EFQTKDSKGNIRNYEMVDGGVAANNPTLAAMTHVTKEMSIL-RHRSELLKIKPMETERML 240
            F+TKD  G  R + +VDGGVAANNPTL AM+ V+K + +  +   +   +KP E  + +
Sbjct: 200 YFETKDDNGQTREFNLVDGGVAANNPTLCAMSQVSKYIILEDKEDCDFFPVKPTEYGKFM 259

Query: 241 VLSLGTGTPKNDEKYSAAKASKWGMLDWVYHGGGTPIVDIFSDASADMVDYHISSIFQSD 300
           V+S+G G+  +D+KY A  A+KWG+ +W+  G   PI+D+F+ ASADMVD H+  +F + 
Sbjct: 260 VISIGCGS-NHDQKYKAKDAAKWGIFNWLIKGSSAPIIDMFTSASADMVDIHLGVLFSAL 319

Query: 301 HCHKNYLRIQDDTLSGEVSSVDIATEENLLNLIYVGENLLKKPLSRVNLESGKFEPLDAK 360
            C KNYLRIQ D L+G   S+D  ++EN+ NL+ +GE LL K +SRV+LE+G +  +  +
Sbjct: 320 QCEKNYLRIQYDQLTGSAGSIDDCSKENMDNLVKIGEMLLDKNVSRVDLETGHYVDVAGE 379

Query: 361 GTNEQALAEFAKMLSNERKLR 381
           GTN   LA+FAK LS+ER+ R
Sbjct: 380 GTNRDQLAKFAKQLSDERRRR 399

BLAST of Cucsa.250620 vs. Swiss-Prot
Match: PLP2_ARATH (Patatin-like protein 2 OS=Arabidopsis thaliana GN=PLP2 PE=1 SV=1)

HSP 1 Score: 412.1 bits (1058), Expect = 6.5e-114
Identity = 210/380 (55.26%), Postives = 277/380 (72.89%), Query Frame = 1

Query: 1   MITILSIDGGGIRGIIPSIILAFLESKLQELDGPDVRIADYFDVIAGTSTGGLVTSMLTA 60
           ++TILSIDGGGIRG+IP++IL FLES+LQ+LDG + R+ADYFDVIAGTSTGGLVT+MLTA
Sbjct: 18  LVTILSIDGGGIRGLIPAVILGFLESELQKLDGEEARLADYFDVIAGTSTGGLVTAMLTA 77

Query: 61  PDKNNRPLYSASDLALFYIEHAPKIFPQRNYFLCSLVNFFGKVMGPKYNGLYLRSLIKGL 120
           P+K  RPL++AS++  FY+E  PKIFPQ ++   +       + GPKY+G YL  LI   
Sbjct: 78  PNKEGRPLFAASEIKDFYLEQCPKIFPQDHFPFSAAKKLVKSLTGPKYDGKYLHQLIHAK 137

Query: 121 LGDITLKQTLSQVVIPAFDIKLLQPVIFTTIEAKCSELKNPKLADVCISTSAAPTFLPGY 180
           LGD  L QTL+ VVIP FDIK LQP IF++ E K   LK+  LAD+ ISTSAAPT+LP +
Sbjct: 138 LGDTKLSQTLTNVVIPTFDIKHLQPTIFSSYEVKNHPLKDATLADIAISTSAAPTYLPAH 197

Query: 181 EFQTKDSKGNIRNYEMVDGGVAANNPTLAAMTHVTKEMSILRHRSELLKIKPMETERMLV 240
            F+ +D  GN + Y ++DGGVAANNP L A+  VT E+S     S+   I+P +  R LV
Sbjct: 198 FFKVEDLNGNAKEYNLIDGGVAANNPALLAIGEVTNEIS--GGSSDFFPIRPNDYGRFLV 257

Query: 241 LSLGTGTPKNDEKYSAAKASKWGMLDWVYHGGGTPIVDIFSDASADMVDYHISSIFQSDH 300
           LSLGTG  K +EK++A + + WG+L+W+ H   TPI+D FS AS+DMVD+H+S++F++ H
Sbjct: 258 LSLGTGNHKAEEKFNAKEVAGWGLLNWLTHDNSTPIIDAFSQASSDMVDFHLSAVFRALH 317

Query: 301 CHKNYLRIQDDTLSGEVSSVDIATEENLLNLIYVGENLLKKPLSRVNLESGKFEPLDAKG 360
              NY+RIQDDTL+G+ +SVDIAT ENL  L   G+ LLKKP++RVNL+SG  E    + 
Sbjct: 318 SEANYIRIQDDTLTGDAASVDIATVENLDILAKTGDELLKKPVARVNLDSGCNENA-YET 377

Query: 361 TNEQALAEFAKMLSNERKLR 381
           TNE AL + A +LS E+K+R
Sbjct: 378 TNEHALIKLAGILSKEKKIR 394

BLAST of Cucsa.250620 vs. Swiss-Prot
Match: PLP1_ARATH (Patatin-like protein 1 OS=Arabidopsis thaliana GN=PLP1 PE=1 SV=2)

HSP 1 Score: 405.2 bits (1040), Expect = 7.9e-112
Identity = 197/380 (51.84%), Postives = 275/380 (72.37%), Query Frame = 1

Query: 1   MITILSIDGGGIRGIIPSIILAFLESKLQELDGPDVRIADYFDVIAGTSTGGLVTSMLTA 60
           ++TILS+DGGG+RGII  +ILAFLE +LQELDG + R+ADYFDVIAGTSTGGLVT+MLT 
Sbjct: 18  LVTILSLDGGGVRGIIAGVILAFLEKQLQELDGEEARLADYFDVIAGTSTGGLVTAMLTV 77

Query: 61  PDKNNRPLYSASDLALFYIEHAPKIFPQRNYFLCSLVNFFGKVMGPKYNGLYLRSLIKGL 120
           PD+  RP ++A D+  FY+EH PKIFPQ    L  L      + GPKY+G YLR+L+  L
Sbjct: 78  PDETGRPHFAAKDIVPFYLEHCPKIFPQPTGVLALLPKLPKLLSGPKYSGKYLRNLLSKL 137

Query: 121 LGDITLKQTLSQVVIPAFDIKLLQPVIFTTIEAKCSELKNPKLADVCISTSAAPTFLPGY 180
           LG+  L QTL+ +VIP FDIK LQP IF++ +       + K++D+CI TSAAPTF P +
Sbjct: 138 LGETRLHQTLTNIVIPTFDIKKLQPTIFSSYQLLVDPSLDVKVSDICIGTSAAPTFFPPH 197

Query: 181 EFQTKDSKGNIRNYEMVDGGVAANNPTLAAMTHVTKEMSILRHRSELLKIKPMETERMLV 240
            F  +DS+GN   + +VDG V ANNPTL AMT V+K+  I+++  ++ K+KP+  +R LV
Sbjct: 198 YFSNEDSQGNKTEFNLVDGAVTANNPTLVAMTAVSKQ--IVKNNPDMGKLKPLGFDRFLV 257

Query: 241 LSLGTGTPKNDEKYSAAKASKWGMLDWVYHGGGTPIVDIFSDASADMVDYHISSIFQSDH 300
           +S+GTG+ K +EKYSA KA+KWG++ W+Y  G TPI+DI  ++S DM+ YH S +F++  
Sbjct: 258 ISIGTGSTKREEKYSAKKAAKWGIISWLYDDGSTPILDITMESSRDMIHYHSSVVFKALQ 317

Query: 301 CHKNYLRIQDDTLSGEVSSVDIATEENLLNLIYVGENLLKKPLSRVNLESGKFEPLDAKG 360
               YLRI DDTL G+VS++D+AT+ NL NL  +GE +L   + ++N+++G +EP+    
Sbjct: 318 SEDKYLRIDDDTLEGDVSTMDLATKSNLENLQKIGEKMLTNRVMQMNIDTGVYEPVAENI 377

Query: 361 TNEQALAEFAKMLSNERKLR 381
           TN++ L  +AK+LS+ERKLR
Sbjct: 378 TNDEQLKRYAKILSDERKLR 395

BLAST of Cucsa.250620 vs. TrEMBL
Match: O23784_CUCSA (Patatin (Fragment) OS=Cucumis sativus PE=2 SV=1)

HSP 1 Score: 759.2 bits (1959), Expect = 2.4e-216
Identity = 383/383 (100.00%), Postives = 383/383 (100.00%), Query Frame = 1

Query: 1   MITILSIDGGGIRGIIPSIILAFLESKLQELDGPDVRIADYFDVIAGTSTGGLVTSMLTA 60
           MITILSIDGGGIRGIIPSIILAFLESKLQELDGPDVRIADYFDVIAGTSTGGLVTSMLTA
Sbjct: 23  MITILSIDGGGIRGIIPSIILAFLESKLQELDGPDVRIADYFDVIAGTSTGGLVTSMLTA 82

Query: 61  PDKNNRPLYSASDLALFYIEHAPKIFPQRNYFLCSLVNFFGKVMGPKYNGLYLRSLIKGL 120
           PDKNNRPLYSASDLALFYIEHAPKIFPQRNYFLCSLVNFFGKVMGPKYNGLYLRSLIKGL
Sbjct: 83  PDKNNRPLYSASDLALFYIEHAPKIFPQRNYFLCSLVNFFGKVMGPKYNGLYLRSLIKGL 142

Query: 121 LGDITLKQTLSQVVIPAFDIKLLQPVIFTTIEAKCSELKNPKLADVCISTSAAPTFLPGY 180
           LGDITLKQTLSQVVIPAFDIKLLQPVIFTTIEAKCSELKNPKLADVCISTSAAPTFLPGY
Sbjct: 143 LGDITLKQTLSQVVIPAFDIKLLQPVIFTTIEAKCSELKNPKLADVCISTSAAPTFLPGY 202

Query: 181 EFQTKDSKGNIRNYEMVDGGVAANNPTLAAMTHVTKEMSILRHRSELLKIKPMETERMLV 240
           EFQTKDSKGNIRNYEMVDGGVAANNPTLAAMTHVTKEMSILRHRSELLKIKPMETERMLV
Sbjct: 203 EFQTKDSKGNIRNYEMVDGGVAANNPTLAAMTHVTKEMSILRHRSELLKIKPMETERMLV 262

Query: 241 LSLGTGTPKNDEKYSAAKASKWGMLDWVYHGGGTPIVDIFSDASADMVDYHISSIFQSDH 300
           LSLGTGTPKNDEKYSAAKASKWGMLDWVYHGGGTPIVDIFSDASADMVDYHISSIFQSDH
Sbjct: 263 LSLGTGTPKNDEKYSAAKASKWGMLDWVYHGGGTPIVDIFSDASADMVDYHISSIFQSDH 322

Query: 301 CHKNYLRIQDDTLSGEVSSVDIATEENLLNLIYVGENLLKKPLSRVNLESGKFEPLDAKG 360
           CHKNYLRIQDDTLSGEVSSVDIATEENLLNLIYVGENLLKKPLSRVNLESGKFEPLDAKG
Sbjct: 323 CHKNYLRIQDDTLSGEVSSVDIATEENLLNLIYVGENLLKKPLSRVNLESGKFEPLDAKG 382

Query: 361 TNEQALAEFAKMLSNERKLRLSP 384
           TNEQALAEFAKMLSNERKLRLSP
Sbjct: 383 TNEQALAEFAKMLSNERKLRLSP 405

BLAST of Cucsa.250620 vs. TrEMBL
Match: A0A0A0LM20_CUCSA (Patatin OS=Cucumis sativus GN=Csa_2G294910 PE=3 SV=1)

HSP 1 Score: 584.7 bits (1506), Expect = 8.1e-164
Identity = 293/294 (99.66%), Postives = 294/294 (100.00%), Query Frame = 1

Query: 90  NYFLCSLVNFFGKVMGPKYNGLYLRSLIKGLLGDITLKQTLSQVVIPAFDIKLLQPVIFT 149
           +YFLCSLVNFFGKVMGPKYNGLYLRSLIKGLLGDITLKQTLSQVVIPAFDIKLLQPVIFT
Sbjct: 8   SYFLCSLVNFFGKVMGPKYNGLYLRSLIKGLLGDITLKQTLSQVVIPAFDIKLLQPVIFT 67

Query: 150 TIEAKCSELKNPKLADVCISTSAAPTFLPGYEFQTKDSKGNIRNYEMVDGGVAANNPTLA 209
           TIEAKCSELKNPKLADVCISTSAAPTFLPGYEFQTKDSKGNIRNYEMVDGGVAANNPTLA
Sbjct: 68  TIEAKCSELKNPKLADVCISTSAAPTFLPGYEFQTKDSKGNIRNYEMVDGGVAANNPTLA 127

Query: 210 AMTHVTKEMSILRHRSELLKIKPMETERMLVLSLGTGTPKNDEKYSAAKASKWGMLDWVY 269
           AMTHVTKEMSILRHRSELLKIKPMETERMLVLSLGTGTPKNDEKYSAAKASKWGMLDWVY
Sbjct: 128 AMTHVTKEMSILRHRSELLKIKPMETERMLVLSLGTGTPKNDEKYSAAKASKWGMLDWVY 187

Query: 270 HGGGTPIVDIFSDASADMVDYHISSIFQSDHCHKNYLRIQDDTLSGEVSSVDIATEENLL 329
           HGGGTPIVDIFSDASADMVDYHISSIFQSDHCHKNYLRIQDDTLSGEVSSVDIATEENLL
Sbjct: 188 HGGGTPIVDIFSDASADMVDYHISSIFQSDHCHKNYLRIQDDTLSGEVSSVDIATEENLL 247

Query: 330 NLIYVGENLLKKPLSRVNLESGKFEPLDAKGTNEQALAEFAKMLSNERKLRLSP 384
           NLIYVGENLLKKPLSRVNLESGKFEPLDAKGTNEQALAEFAKMLSNERKLRLSP
Sbjct: 248 NLIYVGENLLKKPLSRVNLESGKFEPLDAKGTNEQALAEFAKMLSNERKLRLSP 301

BLAST of Cucsa.250620 vs. TrEMBL
Match: W9S7S9_9ROSA (Patatin OS=Morus notabilis GN=L484_003958 PE=3 SV=1)

HSP 1 Score: 496.9 bits (1278), Expect = 2.2e-137
Identity = 240/382 (62.83%), Postives = 312/382 (81.68%), Query Frame = 1

Query: 1   MITILSIDGGGIRGIIPSIILAFLESKLQELDGPDVRIADYFDVIAGTSTGGLVTSMLTA 60
           M T+LSIDGGGIRGIIP+I LAFLE KLQELDGP+ RIADYFD+IAGTSTGGLVT+MLTA
Sbjct: 11  MFTVLSIDGGGIRGIIPAIHLAFLEFKLQELDGPNARIADYFDIIAGTSTGGLVTTMLTA 70

Query: 61  PDKNNRPLYSASDLALFYIEHAPKIFPQR--NYFLCSLVNFFGKVMGPKYNGLYLRSLIK 120
           P+K+NRP+Y+A D+  FY+EH+PKIFPQR  N FL SL +FFG V+GPKY+G YLRS+  
Sbjct: 71  PNKDNRPMYAAKDIPGFYLEHSPKIFPQRRGNNFLTSLASFFGTVIGPKYDGKYLRSMTN 130

Query: 121 GLLGDITLKQTLSQVVIPAFDIKLLQPVIFTTIEAKCSELKNPKLADVCISTSAAPTFLP 180
            LLGD+TLK+TL+ V+IPAFD+KLLQPV F+T +AK + L+N +LAD+C++TSAAPTFLP
Sbjct: 131 SLLGDLTLKETLTNVLIPAFDVKLLQPVFFSTNDAKENALRNARLADICVATSAAPTFLP 190

Query: 181 GYEFQTKDSKGNIRNYEMVDGGVAANNPTLAAMTHVTKEMSILRHRSELLKIKPMETERM 240
            + F T D  G  R+++++DGGVAANNPT+ A+ ++++ ++I     E   +KPM+ ER 
Sbjct: 191 AHFFTTSDKDGKTRSFDLIDGGVAANNPTMLAINYISRGIAI--KNEEFNDMKPMDGERT 250

Query: 241 LVLSLGTGTPKNDEKYSAAKASKWGMLDWVYHGGGTPIVDIFSDASADMVDYHISSIFQS 300
           LVLSLGTGTPKN+EKY+AA ASKWG   WV+  G TP+VDIF+DAS+D+VD+H+S++FQS
Sbjct: 251 LVLSLGTGTPKNEEKYNAAMASKWGAFSWVFENGRTPLVDIFADASSDVVDFHVSTLFQS 310

Query: 301 DHCHKNYLRIQDDTLSGEVSSVDIATEENLLNLIYVGENLLKKPLSRVNLESGKFEPLDA 360
             C KNYLRIQDDTL+G  +SVDIAT +N+ +L  +G+ LL+KP+SRVNL++G++E +  
Sbjct: 311 LRCKKNYLRIQDDTLTGNGASVDIATPQNMQSLAEIGKKLLEKPISRVNLDTGRYEEIKG 370

Query: 361 KGTNEQALAEFAKMLSNERKLR 381
           +G N +ALA FAK LS ER LR
Sbjct: 371 EGINAEALAHFAKRLSEERTLR 390

BLAST of Cucsa.250620 vs. TrEMBL
Match: A0A061DHR7_THECC (Patatin OS=Theobroma cacao GN=TCM_000569 PE=3 SV=1)

HSP 1 Score: 495.7 bits (1275), Expect = 4.9e-137
Identity = 248/386 (64.25%), Postives = 310/386 (80.31%), Query Frame = 1

Query: 1   MITILSIDGGGIRGIIPSIILAFLESKLQELDGPDVRIADYFDVIAGTSTGGLVTSMLTA 60
           M+T+LSIDGGG+RGIIP  +L+FLESKL ELDG + RIADYFD+IAGTSTGGL+ +MLTA
Sbjct: 10  MVTVLSIDGGGVRGIIPGTLLSFLESKLHELDGANARIADYFDIIAGTSTGGLIATMLTA 69

Query: 61  PDKNNRPLYSASDLALFYIEHAPKIFPQ--RNYFLCSLVNFFGKV-MGPKYNGLYLRSLI 120
           P+K NRP+Y+A D+  FY+EH PKIFPQ  R  FL S+ +FF    MGPKY+G YLRSL 
Sbjct: 70  PNKQNRPMYAAKDINNFYLEHCPKIFPQNSRLNFLASMASFFRATSMGPKYDGKYLRSLT 129

Query: 121 KGLLGDITLKQTLSQVVIPAFDIKLLQPVIFTTIEAKCSELKNPKLADVCISTSAAPTFL 180
             LL DITLKQTL+ VVIPAFDIKLLQPVIF+T +AK   LKN +LADVC+ TSAAPTFL
Sbjct: 130 NQLLEDITLKQTLTNVVIPAFDIKLLQPVIFSTDDAKADALKNARLADVCVGTSAAPTFL 189

Query: 181 PGYEFQTKDSKGNIRNYEMVDGGVAANNPTLAAMTHVTKEMSILRHRSELLKIKPMETER 240
           P + F+TK++ G  R ++++DGGVAANNPT+ A+ H +KE  IL+H +E ++I+PM++ R
Sbjct: 190 PAHYFETKNADGKTRTFDLIDGGVAANNPTMLAINHFSKE--ILKHGTEFVEIEPMDSRR 249

Query: 241 MLVLSLGTGTPKNDEKYSAAKASKWGMLDWVYHGGGTPIVDIFSDASADMVDYHISSIFQ 300
           MLVLSLGTG  K++EKYSAA ASKWGM+DW+Y  G TP++D+F DAS+D+VD+H S+ FQ
Sbjct: 250 MLVLSLGTGEAKHEEKYSAAAASKWGMIDWLYEKGKTPLLDVFWDASSDIVDFHTSTQFQ 309

Query: 301 SDHCHKNYLRIQDDTLSGEVSSVDIATEENLLNLIYVGENLLKKPLSRVNLESGKFEPLD 360
             +   NYLRIQDDT +G+ +SVDIATEENL  LI +G  LLKK +SRVNLE+GKFE ++
Sbjct: 310 CLNSKDNYLRIQDDTFTGDAASVDIATEENLKKLIGIGTELLKKRVSRVNLETGKFEEVE 369

Query: 361 AKGTNEQALAEFAKMLSNERKLRLSP 384
            + TNE ALA FAK LS ERKLRL+P
Sbjct: 370 GEPTNEVALANFAKKLSEERKLRLAP 393

BLAST of Cucsa.250620 vs. TrEMBL
Match: W9SE91_9ROSA (Patatin OS=Morus notabilis GN=L484_013239 PE=3 SV=1)

HSP 1 Score: 487.3 bits (1253), Expect = 1.8e-134
Identity = 239/384 (62.24%), Postives = 312/384 (81.25%), Query Frame = 1

Query: 1   MITILSIDGGGIRGIIPSIILAFLESKLQELDGPDVRIADYFDVIAGTSTGGLVTSMLTA 60
           + T+LSIDGGGIRGIIP   LAFLESKLQELDGPDVRIADYFD+I GTSTGGLVT+MLTA
Sbjct: 11  VFTVLSIDGGGIRGIIPGTHLAFLESKLQELDGPDVRIADYFDIITGTSTGGLVTAMLTA 70

Query: 61  PDKNNRPLYSASDLALFYIEHAPKIFPQ--RNYFLCSLVNFFGKVMGPKYNGLYLRSLIK 120
           PDK+NRPLY+A D+  FY+EHAPKIFPQ  RN F+ +L++ F    GPKY+G YL+SL+ 
Sbjct: 71  PDKDNRPLYAAKDITDFYLEHAPKIFPQQNRNNFIGNLISTF---TGPKYDGKYLKSLLN 130

Query: 121 GLLGDITLKQTLSQVVIPAFDIKLLQPVIFTTIEAKCSELKNPKLADVCISTSAAPTFLP 180
            LL ++TLKQTL+ V+IP FDIKLLQP+IF+T +AK + L N +++D+CI TSAAPTFLP
Sbjct: 131 DLLSNLTLKQTLTDVLIPTFDIKLLQPIIFSTNDAKENALSNARISDICIGTSAAPTFLP 190

Query: 181 GYEFQTKDSKGNIRNYEMVDGGVAANNPTLAAMTHVTKEMSILRHRSELLKIKPMETERM 240
            + F T D KGN RN+E++DGGVAANNPT+ A++H++KE  I R  SE   +K M++ RM
Sbjct: 191 AHSFTTSD-KGNSRNFELIDGGVAANNPTMLAISHISKE--IARKNSEFKDMKSMDSNRM 250

Query: 241 LVLSLGTGTPKNDEKYSAAKASKWGMLDWVYHGGGTPIVDIFSDASADMVDYHISSIFQS 300
           LVLSLGTG  K + KYSA+KASKWG+++W++  G TP++DIF D+++D+VD+H+S+IFQ+
Sbjct: 251 LVLSLGTGAAKQEMKYSASKASKWGLINWIFDNGSTPLIDIFFDSNSDIVDFHLSAIFQA 310

Query: 301 DHCHKNYLRIQDDTLSGEVSSVDIATEENLLNLIYVGENLLKKPLSRVNLESGKFEPLDA 360
            +  +NYLRIQ DTL+G  SSVD++TEENL  L+ +G+ +L+KP+SRVNLE+GK+E  + 
Sbjct: 311 QNSKENYLRIQYDTLTGNESSVDVSTEENLKRLVEIGKEMLEKPVSRVNLETGKYETAEG 370

Query: 361 KGTNEQALAEFAKMLSNERKLRLS 383
           +GTN +AL EFAK+LS ERK R S
Sbjct: 371 EGTNAEALTEFAKLLSEERKHRRS 388

BLAST of Cucsa.250620 vs. TAIR10
Match: AT4G37050.1 (AT4G37050.1 PATATIN-like protein 4)

HSP 1 Score: 421.4 bits (1082), Expect = 6.0e-118
Identity = 213/393 (54.20%), Postives = 284/393 (72.26%), Query Frame = 1

Query: 1   MITILSIDGGGIRGIIPSIILAFLESKLQELDGPDVRIADYFDVIAGTSTGGLVTSMLTA 60
           ++TILSIDGGGIRGIIP  ILA+LES+LQELDG + R+ DYFDVI+GTSTGGL+ +MLTA
Sbjct: 34  LVTILSIDGGGIRGIIPGTILAYLESQLQELDGEEARLVDYFDVISGTSTGGLIVAMLTA 93

Query: 61  PDKN-------NRPLYSASDLALFYIEHAPKIFPQRNYFLC----SLVNFFGKVMGPKYN 120
            D++       NRPL+ A ++  FY++H+PKIFPQ     C    ++V   G   GPK+N
Sbjct: 94  QDQSGGHSRNSNRPLFEAKEIVPFYLKHSPKIFPQPRGIFCGWGETIVRLVG---GPKFN 153

Query: 121 GLYLRSLIKGLLGDITLKQTLSQVVIPAFDIKLLQPVIFTTIEAKCSELKNPKLADVCIS 180
           G YL  L++G LGD  L Q+L+ VVIP FDIK LQPVIF++ +A  ++  N KL+D+CIS
Sbjct: 154 GKYLHDLVEGFLGDTKLTQSLTNVVIPCFDIKKLQPVIFSSYQAVNNQAMNAKLSDICIS 213

Query: 181 TSAAPTFLPGYEFQTKDSKGNIRNYEMVDGGVAANNPTLAAMTHVTKEMSILRHRSELLK 240
           TSAAPTF P + F  +DS+G    + ++DGG+AANNPTL A+  VTK+  I++    +  
Sbjct: 214 TSAAPTFFPAHRFTNEDSEGIKHEFNLIDGGIAANNPTLCAIAEVTKQ--IIKKNPVMGD 273

Query: 241 IKPMETERMLVLSLGTGTPKNDEKYSAAKASKWGMLDWVYHGGGTPIVDIFSDASADMVD 300
           I P++  R LV+S+GTG+ +N EKY+A  ASKWG++ WV+  G TPI+D +S+A  DMVD
Sbjct: 274 ISPLDFTRFLVISIGTGSIRNQEKYNAKMASKWGLMCWVFESGSTPILDCYSEAIHDMVD 333

Query: 301 YHISSIFQSDHCHKNYLRIQDDTLSGEVSSVDIATEENLLNLIYVGENLLKKPLSRVNLE 360
           Y  S +FQ+    KNYLRI DD+L G++ SVDI+TE+N+  L+ VGE LLKK +SRVNLE
Sbjct: 334 YQSSVVFQALRSEKNYLRIDDDSLKGDLGSVDISTEKNMEGLVEVGEALLKKRVSRVNLE 393

Query: 361 SGKFEPLDAKGTNEQALAEFAKMLSNERKLRLS 383
           SG ++P+    TNE+AL  FAK+LS ERKLR S
Sbjct: 394 SGHYQPISENVTNEEALKRFAKVLSEERKLRES 421

BLAST of Cucsa.250620 vs. TAIR10
Match: AT2G26560.1 (AT2G26560.1 phospholipase A 2A)

HSP 1 Score: 412.1 bits (1058), Expect = 3.6e-115
Identity = 210/380 (55.26%), Postives = 277/380 (72.89%), Query Frame = 1

Query: 1   MITILSIDGGGIRGIIPSIILAFLESKLQELDGPDVRIADYFDVIAGTSTGGLVTSMLTA 60
           ++TILSIDGGGIRG+IP++IL FLES+LQ+LDG + R+ADYFDVIAGTSTGGLVT+MLTA
Sbjct: 18  LVTILSIDGGGIRGLIPAVILGFLESELQKLDGEEARLADYFDVIAGTSTGGLVTAMLTA 77

Query: 61  PDKNNRPLYSASDLALFYIEHAPKIFPQRNYFLCSLVNFFGKVMGPKYNGLYLRSLIKGL 120
           P+K  RPL++AS++  FY+E  PKIFPQ ++   +       + GPKY+G YL  LI   
Sbjct: 78  PNKEGRPLFAASEIKDFYLEQCPKIFPQDHFPFSAAKKLVKSLTGPKYDGKYLHQLIHAK 137

Query: 121 LGDITLKQTLSQVVIPAFDIKLLQPVIFTTIEAKCSELKNPKLADVCISTSAAPTFLPGY 180
           LGD  L QTL+ VVIP FDIK LQP IF++ E K   LK+  LAD+ ISTSAAPT+LP +
Sbjct: 138 LGDTKLSQTLTNVVIPTFDIKHLQPTIFSSYEVKNHPLKDATLADIAISTSAAPTYLPAH 197

Query: 181 EFQTKDSKGNIRNYEMVDGGVAANNPTLAAMTHVTKEMSILRHRSELLKIKPMETERMLV 240
            F+ +D  GN + Y ++DGGVAANNP L A+  VT E+S     S+   I+P +  R LV
Sbjct: 198 FFKVEDLNGNAKEYNLIDGGVAANNPALLAIGEVTNEIS--GGSSDFFPIRPNDYGRFLV 257

Query: 241 LSLGTGTPKNDEKYSAAKASKWGMLDWVYHGGGTPIVDIFSDASADMVDYHISSIFQSDH 300
           LSLGTG  K +EK++A + + WG+L+W+ H   TPI+D FS AS+DMVD+H+S++F++ H
Sbjct: 258 LSLGTGNHKAEEKFNAKEVAGWGLLNWLTHDNSTPIIDAFSQASSDMVDFHLSAVFRALH 317

Query: 301 CHKNYLRIQDDTLSGEVSSVDIATEENLLNLIYVGENLLKKPLSRVNLESGKFEPLDAKG 360
              NY+RIQDDTL+G+ +SVDIAT ENL  L   G+ LLKKP++RVNL+SG  E    + 
Sbjct: 318 SEANYIRIQDDTLTGDAASVDIATVENLDILAKTGDELLKKPVARVNLDSGCNENA-YET 377

Query: 361 TNEQALAEFAKMLSNERKLR 381
           TNE AL + A +LS E+K+R
Sbjct: 378 TNEHALIKLAGILSKEKKIR 394

BLAST of Cucsa.250620 vs. TAIR10
Match: AT4G37070.2 (AT4G37070.2 Acyl transferase/acyl hydrolase/lysophospholipase superfamily protein)

HSP 1 Score: 405.2 bits (1040), Expect = 4.4e-113
Identity = 197/380 (51.84%), Postives = 275/380 (72.37%), Query Frame = 1

Query: 1   MITILSIDGGGIRGIIPSIILAFLESKLQELDGPDVRIADYFDVIAGTSTGGLVTSMLTA 60
           ++TILS+DGGG+RGII  +ILAFLE +LQELDG + R+ADYFDVIAGTSTGGLVT+MLT 
Sbjct: 18  LVTILSLDGGGVRGIIAGVILAFLEKQLQELDGEEARLADYFDVIAGTSTGGLVTAMLTV 77

Query: 61  PDKNNRPLYSASDLALFYIEHAPKIFPQRNYFLCSLVNFFGKVMGPKYNGLYLRSLIKGL 120
           PD+  RP ++A D+  FY+EH PKIFPQ    L  L      + GPKY+G YLR+L+  L
Sbjct: 78  PDETGRPHFAAKDIVPFYLEHCPKIFPQPTGVLALLPKLPKLLSGPKYSGKYLRNLLSKL 137

Query: 121 LGDITLKQTLSQVVIPAFDIKLLQPVIFTTIEAKCSELKNPKLADVCISTSAAPTFLPGY 180
           LG+  L QTL+ +VIP FDIK LQP IF++ +       + K++D+CI TSAAPTF P +
Sbjct: 138 LGETRLHQTLTNIVIPTFDIKKLQPTIFSSYQLLVDPSLDVKVSDICIGTSAAPTFFPPH 197

Query: 181 EFQTKDSKGNIRNYEMVDGGVAANNPTLAAMTHVTKEMSILRHRSELLKIKPMETERMLV 240
            F  +DS+GN   + +VDG V ANNPTL AMT V+K+  I+++  ++ K+KP+  +R LV
Sbjct: 198 YFSNEDSQGNKTEFNLVDGAVTANNPTLVAMTAVSKQ--IVKNNPDMGKLKPLGFDRFLV 257

Query: 241 LSLGTGTPKNDEKYSAAKASKWGMLDWVYHGGGTPIVDIFSDASADMVDYHISSIFQSDH 300
           +S+GTG+ K +EKYSA KA+KWG++ W+Y  G TPI+DI  ++S DM+ YH S +F++  
Sbjct: 258 ISIGTGSTKREEKYSAKKAAKWGIISWLYDDGSTPILDITMESSRDMIHYHSSVVFKALQ 317

Query: 301 CHKNYLRIQDDTLSGEVSSVDIATEENLLNLIYVGENLLKKPLSRVNLESGKFEPLDAKG 360
               YLRI DDTL G+VS++D+AT+ NL NL  +GE +L   + ++N+++G +EP+    
Sbjct: 318 SEDKYLRIDDDTLEGDVSTMDLATKSNLENLQKIGEKMLTNRVMQMNIDTGVYEPVAENI 377

Query: 361 TNEQALAEFAKMLSNERKLR 381
           TN++ L  +AK+LS+ERKLR
Sbjct: 378 TNDEQLKRYAKILSDERKLR 395

BLAST of Cucsa.250620 vs. TAIR10
Match: AT4G37060.2 (AT4G37060.2 PATATIN-like protein 5)

HSP 1 Score: 386.0 bits (990), Expect = 2.8e-107
Identity = 196/411 (47.69%), Postives = 274/411 (66.67%), Query Frame = 1

Query: 1   MITILSIDGGGIRGIIPSIILAFLESKLQ------------------------------- 60
           ++TILS+DGGG+RGII  +ILA+LE +LQ                               
Sbjct: 8   LVTILSLDGGGVRGIIAGVILAYLEKQLQVYIYINFSRMQVTKIVDTYDRIITRHANFVV 67

Query: 61  ELDGPDVRIADYFDVIAGTSTGGLVTSMLTAPDKNNRPLYSASDLALFYIEHAPKIFPQR 120
           ELDG  VR+ADYFDVIAGTSTGGLVT+MLTAPD+N RP ++A ++  FY+EH PKIFPQ 
Sbjct: 68  ELDGEHVRVADYFDVIAGTSTGGLVTAMLTAPDENGRPRFAAKEIVPFYLEHCPKIFPQP 127

Query: 121 NYFLCSLVNFFGKVMGPKYNGLYLRSLIKGLLGDITLKQTLSQVVIPAFDIKLLQPVIFT 180
              L  L      + GPKY+G YLR+ +  LLG+  L+QTL+ VVIP FDIK LQP IF+
Sbjct: 128 TGVLALLPKLPKLLSGPKYSGNYLRTTLGKLLGETKLRQTLTNVVIPTFDIKTLQPTIFS 187

Query: 181 TIEAKCSELKNPKLADVCISTSAAPTFLPGYEFQTKDSKGNIRNYEMVDGGVAANNPTLA 240
           + +A      + K++D+CI TSAAPT+ P Y F  +DS+G  R++ +VDGGV ANNPTL 
Sbjct: 188 SYQALTDPSLDVKVSDICIGTSAAPTYFPPYYFSNEDSQGKTRHFNLVDGGVTANNPTLV 247

Query: 241 AMTHVTKEMSILRHRSELLKIKPMETERMLVLSLGTGTPKNDEKYSAAKASKWGMLDWVY 300
           AMT VTK+  I+ +  ++  + P+  ++ LV+S+GTG+ K +E+YSA KA+KWG++ W+Y
Sbjct: 248 AMTAVTKQ--IVNNNPDMGTLNPLGYDQFLVISIGTGSAKKEERYSAKKAAKWGIISWLY 307

Query: 301 HGGGTPIVDIFSDASADMVDYHISSIFQSDHCHKNYLRIQDDTLSGEVSSVDIATEENLL 360
             G TPI+DI  ++S D+V YH S +F++      YLRI DDTL G+ S++D++T+ NL 
Sbjct: 308 EDGTTPILDITFESSRDIVHYHSSVVFKALQSEDKYLRIDDDTLEGDASTLDLSTKSNLE 367

Query: 361 NLIYVGENLLKKPLSRVNLESGKFEPLDAKGTNEQALAEFAKMLSNERKLR 381
           NLI +GE +L   + ++N+++G +EP      N++ L  FAK+LS ERKLR
Sbjct: 368 NLIKLGEKMLTNRVMQMNIDTGTYEPAAENINNDEQLKRFAKILSEERKLR 416

BLAST of Cucsa.250620 vs. TAIR10
Match: AT5G43590.1 (AT5G43590.1 Acyl transferase/acyl hydrolase/lysophospholipase superfamily protein)

HSP 1 Score: 339.7 bits (870), Expect = 2.3e-93
Identity = 184/383 (48.04%), Postives = 258/383 (67.36%), Query Frame = 1

Query: 1   MITILSIDGGGIRGIIPSIILAFLESKLQELDGPD-VRIADYFDVIAGTSTGGLVTSMLT 60
           ++TILS+DGGG+RGII  +ILA LE  LQE+D  + VR+ADYFDVIAGTSTGGL+T+MLT
Sbjct: 13  LVTILSLDGGGVRGIIGGVILANLEKHLQEIDNDESVRLADYFDVIAGTSTGGLMTAMLT 72

Query: 61  APDKNNRPLYSASDLALFYIEHAPKIFPQRNYFLCSLVNFFGKVMGPKYNGLYLRSLIKG 120
           AP+ + RPLY+A D+  FY+E +PKIF    ++  S +     +  PKYNG YL + +  
Sbjct: 73  APNDSGRPLYAAKDIVPFYLEESPKIFYGSKWWDPSAL---WALFRPKYNGEYLHTRLGE 132

Query: 121 LLGDITLKQTLSQVVIPAFDIKLLQPVIFTTIEAKCSELKNPKLADVCISTSAAPTFLPG 180
           +LG+  L QTL+ VVIP FDIK LQP IF++  A      N KL+D+CI TSAAP +LP 
Sbjct: 133 ILGETKLDQTLTNVVIPTFDIKKLQPTIFSSYHASVDPSLNAKLSDICIGTSAAPFYLPP 192

Query: 181 YEFQTKDSKGNIRNYEMVDGGVAANNPTLAAMTHVTKEMSILRHRSELLKIKPMETERML 240
           Y+F   D    +R + ++DGGV AN+PTL  MT ++++ SI++H  ++   KP+E E+ +
Sbjct: 193 YKFPENDK---MRTFNLIDGGVTANDPTLVGMTAMSRK-SIIKH-PDMDGFKPLEYEKYI 252

Query: 241 VLSLGTGTPKNDEKYSAAKASKWGMLDWVYHGGG--TPIVDIFSDASADMVDYHISSIFQ 300
           V+S+GTG+ K +E YSA +A+KWG  +W Y+     TPI+DI  ++S DMV YH S +FQ
Sbjct: 253 VISIGTGSAKREEYYSAVEAAKWGFENWAYNWKHKTTPILDIIFESSRDMVQYHTSVLFQ 312

Query: 301 SDHCHKNYLRIQDDTLSGEVSSVDIATEENLLNLIYVGENLLKKPLSRVNLESGKFEPLD 360
           +     NYLRI  DTL  +   +D +   NL NL  +GE LL   + R+NL++  +EP+ 
Sbjct: 313 ALESEDNYLRIDADTLKKDEVFMDDSETLNLENLKNIGEKLLDTNVMRMNLDTYTYEPIP 372

Query: 361 AKGTNEQALAEFAKMLSNERKLR 381
               N+Q L  FAK+LS+E+KLR
Sbjct: 373 KTVNNDQELKRFAKILSDEKKLR 387

BLAST of Cucsa.250620 vs. NCBI nr
Match: gi|778670106|ref|XP_011649370.1| (PREDICTED: patatin-like protein 3 [Cucumis sativus])

HSP 1 Score: 759.2 bits (1959), Expect = 3.4e-216
Identity = 383/383 (100.00%), Postives = 383/383 (100.00%), Query Frame = 1

Query: 1   MITILSIDGGGIRGIIPSIILAFLESKLQELDGPDVRIADYFDVIAGTSTGGLVTSMLTA 60
           MITILSIDGGGIRGIIPSIILAFLESKLQELDGPDVRIADYFDVIAGTSTGGLVTSMLTA
Sbjct: 10  MITILSIDGGGIRGIIPSIILAFLESKLQELDGPDVRIADYFDVIAGTSTGGLVTSMLTA 69

Query: 61  PDKNNRPLYSASDLALFYIEHAPKIFPQRNYFLCSLVNFFGKVMGPKYNGLYLRSLIKGL 120
           PDKNNRPLYSASDLALFYIEHAPKIFPQRNYFLCSLVNFFGKVMGPKYNGLYLRSLIKGL
Sbjct: 70  PDKNNRPLYSASDLALFYIEHAPKIFPQRNYFLCSLVNFFGKVMGPKYNGLYLRSLIKGL 129

Query: 121 LGDITLKQTLSQVVIPAFDIKLLQPVIFTTIEAKCSELKNPKLADVCISTSAAPTFLPGY 180
           LGDITLKQTLSQVVIPAFDIKLLQPVIFTTIEAKCSELKNPKLADVCISTSAAPTFLPGY
Sbjct: 130 LGDITLKQTLSQVVIPAFDIKLLQPVIFTTIEAKCSELKNPKLADVCISTSAAPTFLPGY 189

Query: 181 EFQTKDSKGNIRNYEMVDGGVAANNPTLAAMTHVTKEMSILRHRSELLKIKPMETERMLV 240
           EFQTKDSKGNIRNYEMVDGGVAANNPTLAAMTHVTKEMSILRHRSELLKIKPMETERMLV
Sbjct: 190 EFQTKDSKGNIRNYEMVDGGVAANNPTLAAMTHVTKEMSILRHRSELLKIKPMETERMLV 249

Query: 241 LSLGTGTPKNDEKYSAAKASKWGMLDWVYHGGGTPIVDIFSDASADMVDYHISSIFQSDH 300
           LSLGTGTPKNDEKYSAAKASKWGMLDWVYHGGGTPIVDIFSDASADMVDYHISSIFQSDH
Sbjct: 250 LSLGTGTPKNDEKYSAAKASKWGMLDWVYHGGGTPIVDIFSDASADMVDYHISSIFQSDH 309

Query: 301 CHKNYLRIQDDTLSGEVSSVDIATEENLLNLIYVGENLLKKPLSRVNLESGKFEPLDAKG 360
           CHKNYLRIQDDTLSGEVSSVDIATEENLLNLIYVGENLLKKPLSRVNLESGKFEPLDAKG
Sbjct: 310 CHKNYLRIQDDTLSGEVSSVDIATEENLLNLIYVGENLLKKPLSRVNLESGKFEPLDAKG 369

Query: 361 TNEQALAEFAKMLSNERKLRLSP 384
           TNEQALAEFAKMLSNERKLRLSP
Sbjct: 370 TNEQALAEFAKMLSNERKLRLSP 392

BLAST of Cucsa.250620 vs. NCBI nr
Match: gi|2462265|emb|CAA73328.1| (patatin-like protein [Cucumis sativus])

HSP 1 Score: 759.2 bits (1959), Expect = 3.4e-216
Identity = 383/383 (100.00%), Postives = 383/383 (100.00%), Query Frame = 1

Query: 1   MITILSIDGGGIRGIIPSIILAFLESKLQELDGPDVRIADYFDVIAGTSTGGLVTSMLTA 60
           MITILSIDGGGIRGIIPSIILAFLESKLQELDGPDVRIADYFDVIAGTSTGGLVTSMLTA
Sbjct: 23  MITILSIDGGGIRGIIPSIILAFLESKLQELDGPDVRIADYFDVIAGTSTGGLVTSMLTA 82

Query: 61  PDKNNRPLYSASDLALFYIEHAPKIFPQRNYFLCSLVNFFGKVMGPKYNGLYLRSLIKGL 120
           PDKNNRPLYSASDLALFYIEHAPKIFPQRNYFLCSLVNFFGKVMGPKYNGLYLRSLIKGL
Sbjct: 83  PDKNNRPLYSASDLALFYIEHAPKIFPQRNYFLCSLVNFFGKVMGPKYNGLYLRSLIKGL 142

Query: 121 LGDITLKQTLSQVVIPAFDIKLLQPVIFTTIEAKCSELKNPKLADVCISTSAAPTFLPGY 180
           LGDITLKQTLSQVVIPAFDIKLLQPVIFTTIEAKCSELKNPKLADVCISTSAAPTFLPGY
Sbjct: 143 LGDITLKQTLSQVVIPAFDIKLLQPVIFTTIEAKCSELKNPKLADVCISTSAAPTFLPGY 202

Query: 181 EFQTKDSKGNIRNYEMVDGGVAANNPTLAAMTHVTKEMSILRHRSELLKIKPMETERMLV 240
           EFQTKDSKGNIRNYEMVDGGVAANNPTLAAMTHVTKEMSILRHRSELLKIKPMETERMLV
Sbjct: 203 EFQTKDSKGNIRNYEMVDGGVAANNPTLAAMTHVTKEMSILRHRSELLKIKPMETERMLV 262

Query: 241 LSLGTGTPKNDEKYSAAKASKWGMLDWVYHGGGTPIVDIFSDASADMVDYHISSIFQSDH 300
           LSLGTGTPKNDEKYSAAKASKWGMLDWVYHGGGTPIVDIFSDASADMVDYHISSIFQSDH
Sbjct: 263 LSLGTGTPKNDEKYSAAKASKWGMLDWVYHGGGTPIVDIFSDASADMVDYHISSIFQSDH 322

Query: 301 CHKNYLRIQDDTLSGEVSSVDIATEENLLNLIYVGENLLKKPLSRVNLESGKFEPLDAKG 360
           CHKNYLRIQDDTLSGEVSSVDIATEENLLNLIYVGENLLKKPLSRVNLESGKFEPLDAKG
Sbjct: 323 CHKNYLRIQDDTLSGEVSSVDIATEENLLNLIYVGENLLKKPLSRVNLESGKFEPLDAKG 382

Query: 361 TNEQALAEFAKMLSNERKLRLSP 384
           TNEQALAEFAKMLSNERKLRLSP
Sbjct: 383 TNEQALAEFAKMLSNERKLRLSP 405

BLAST of Cucsa.250620 vs. NCBI nr
Match: gi|659116056|ref|XP_008457877.1| (PREDICTED: LOW QUALITY PROTEIN: patatin-like protein 2 [Cucumis melo])

HSP 1 Score: 730.3 bits (1884), Expect = 1.7e-207
Identity = 367/382 (96.07%), Postives = 376/382 (98.43%), Query Frame = 1

Query: 2   ITILSIDGGGIRGIIPSIILAFLESKLQELDGPDVRIADYFDVIAGTSTGGLVTSMLTAP 61
           ITILSIDGGGIRGIIPSIILAFLE KLQELDGPDVRIADYFDVIAGTSTGGLVTSMLTAP
Sbjct: 11  ITILSIDGGGIRGIIPSIILAFLELKLQELDGPDVRIADYFDVIAGTSTGGLVTSMLTAP 70

Query: 62  DKNNRPLYSASDLALFYIEHAPKIFPQRNYFLCSLVNFFGKVMGPKYNGLYLRSLIKGLL 121
           DKNNRPLY+ASD+ALFYIEHAPKIFPQRNYFLCSLV FFGKVMGPKY+GLYLRSLIKGLL
Sbjct: 71  DKNNRPLYTASDVALFYIEHAPKIFPQRNYFLCSLVXFFGKVMGPKYDGLYLRSLIKGLL 130

Query: 122 GDITLKQTLSQVVIPAFDIKLLQPVIFTTIEAKCSELKNPKLADVCISTSAAPTFLPGYE 181
           GDITLKQTL+QVVIPAFDIKLLQPVIFTTI+AKCSELKNPKLADVCISTSAAPTFLPGYE
Sbjct: 131 GDITLKQTLTQVVIPAFDIKLLQPVIFTTIDAKCSELKNPKLADVCISTSAAPTFLPGYE 190

Query: 182 FQTKDSKGNIRNYEMVDGGVAANNPTLAAMTHVTKEMSILRHRSELLKIKPMETERMLVL 241
           FQTKDSKGNIRNYEMVDGGVAANNPTLAAMTHVTKEMSILRHRSELLKIKPMET+RMLVL
Sbjct: 191 FQTKDSKGNIRNYEMVDGGVAANNPTLAAMTHVTKEMSILRHRSELLKIKPMETKRMLVL 250

Query: 242 SLGTGTPKNDEKYSAAKASKWGMLDWVYHGGGTPIVDIFSDASADMVDYHISSIFQSDHC 301
           SLGTGTPKNDEKYSAAKASKWGMLDWVYHGG TPIVDIFSDASADMVDYHISSIFQSDHC
Sbjct: 251 SLGTGTPKNDEKYSAAKASKWGMLDWVYHGGATPIVDIFSDASADMVDYHISSIFQSDHC 310

Query: 302 HKNYLRIQDDTLSGEVSSVDIATEENLLNLIYVGENLLKKPLSRVNLESGKFEPLDAKGT 361
           HKNYLRIQDDTLSGEVSSVDIAT ENLL LIYVGE+LLKKPLSRVNLESGKFEPLDA+GT
Sbjct: 311 HKNYLRIQDDTLSGEVSSVDIATAENLLKLIYVGEDLLKKPLSRVNLESGKFEPLDAQGT 370

Query: 362 NEQALAEFAKMLSNERKLRLSP 384
           NEQAL EFAKMLS+ERKLRLSP
Sbjct: 371 NEQALTEFAKMLSDERKLRLSP 392

BLAST of Cucsa.250620 vs. NCBI nr
Match: gi|659116048|ref|XP_008457873.1| (PREDICTED: patatin-like protein 2 [Cucumis melo])

HSP 1 Score: 647.9 bits (1670), Expect = 1.1e-182
Identity = 324/382 (84.82%), Postives = 353/382 (92.41%), Query Frame = 1

Query: 2   ITILSIDGGGIRGIIPSIILAFLESKLQELDGPDVRIADYFDVIAGTSTGGLVTSMLTAP 61
           ITILSIDGGGIRGIIP  ILAFLESKLQELDGPD RIADYFDVIAGTSTGGLVTSMLTAP
Sbjct: 11  ITILSIDGGGIRGIIPGTILAFLESKLQELDGPDARIADYFDVIAGTSTGGLVTSMLTAP 70

Query: 62  DKNNRPLYSASDLALFYIEHAPKIFPQRNYFLCSLVNFFGKVMGPKYNGLYLRSLIKGLL 121
           ++NNRPLY+A DL  FYIEH PKIFPQRN+FL S+VN FGKVMGPKY+G YLRSLI  LL
Sbjct: 71  NENNRPLYAAKDLTRFYIEHGPKIFPQRNHFLSSMVNMFGKVMGPKYDGKYLRSLINRLL 130

Query: 122 GDITLKQTLSQVVIPAFDIKLLQPVIFTTIEAKCSELKNPKLADVCISTSAAPTFLPGYE 181
           GDITLKQTL++V+IPAFDIKLLQPVIF+T++AK   LKNPKLADVCISTSAAPTFLPG+E
Sbjct: 131 GDITLKQTLTEVIIPAFDIKLLQPVIFSTLDAKWDALKNPKLADVCISTSAAPTFLPGHE 190

Query: 182 FQTKDSKGNIRNYEMVDGGVAANNPTLAAMTHVTKEMSILRHRSELLKIKPMETERMLVL 241
           FQTKDSKGN RN++MVDGGVAANNPTLAA+THVTKEMSILR+RSELLKIKPME +RML+L
Sbjct: 191 FQTKDSKGNTRNFDMVDGGVAANNPTLAALTHVTKEMSILRNRSELLKIKPMEAKRMLIL 250

Query: 242 SLGTGTPKNDEKYSAAKASKWGMLDWVYHGGGTPIVDIFSDASADMVDYHISSIFQSDHC 301
           SLGTG  KNDEKYSAA ASKWGML W+YH G TPIVDIFSDASADMVDYHISSIFQS+H 
Sbjct: 251 SLGTGVAKNDEKYSAAIASKWGMLGWIYHRGATPIVDIFSDASADMVDYHISSIFQSEHN 310

Query: 302 HKNYLRIQDDTLSGEVSSVDIATEENLLNLIYVGENLLKKPLSRVNLESGKFEPLDAKGT 361
            KNYLRIQDDTLSG+VSSVDIAT++NLL LI VGENLLKKPLSRVNLESG FEPLD +GT
Sbjct: 311 QKNYLRIQDDTLSGDVSSVDIATQQNLLKLIEVGENLLKKPLSRVNLESGNFEPLDGEGT 370

Query: 362 NEQALAEFAKMLSNERKLRLSP 384
           NE+ALA+FA+MLS+ERKLRLSP
Sbjct: 371 NEKALADFAQMLSDERKLRLSP 392

BLAST of Cucsa.250620 vs. NCBI nr
Match: gi|778670099|ref|XP_011649366.1| (PREDICTED: patatin-like protein 2 [Cucumis sativus])

HSP 1 Score: 644.8 bits (1662), Expect = 9.4e-182
Identity = 323/382 (84.55%), Postives = 352/382 (92.15%), Query Frame = 1

Query: 2   ITILSIDGGGIRGIIPSIILAFLESKLQELDGPDVRIADYFDVIAGTSTGGLVTSMLTAP 61
           ITILSIDGGGIRGIIP  ILAFLESKLQELDGPD RIADYFDVIAGTSTGGLVTSMLTAP
Sbjct: 14  ITILSIDGGGIRGIIPGTILAFLESKLQELDGPDARIADYFDVIAGTSTGGLVTSMLTAP 73

Query: 62  DKNNRPLYSASDLALFYIEHAPKIFPQRNYFLCSLVNFFGKVMGPKYNGLYLRSLIKGLL 121
           ++NNRPLY+A DL  FYIEH PKIFPQRNYFL S+VN FGKVMGPKY+G YLRSLI  LL
Sbjct: 74  NENNRPLYAAKDLTRFYIEHGPKIFPQRNYFLSSVVNMFGKVMGPKYDGKYLRSLINRLL 133

Query: 122 GDITLKQTLSQVVIPAFDIKLLQPVIFTTIEAKCSELKNPKLADVCISTSAAPTFLPGYE 181
           GDITLK+TL+QV+IPAFDIKLLQPVIF+T++AK   LKNPKLADVCISTSAAPTFLPG+E
Sbjct: 134 GDITLKETLTQVIIPAFDIKLLQPVIFSTLDAKWDALKNPKLADVCISTSAAPTFLPGHE 193

Query: 182 FQTKDSKGNIRNYEMVDGGVAANNPTLAAMTHVTKEMSILRHRSELLKIKPMETERMLVL 241
           FQTKDSKGN RN++MVDGGVAANNPTLAA+THVTKEMSILR RSELLKIKPME +RML+L
Sbjct: 194 FQTKDSKGNTRNFDMVDGGVAANNPTLAALTHVTKEMSILRKRSELLKIKPMEAKRMLIL 253

Query: 242 SLGTGTPKNDEKYSAAKASKWGMLDWVYHGGGTPIVDIFSDASADMVDYHISSIFQSDHC 301
           SLGTG  KNDEKYSAA +SKWGML W+YH G TPIVDIFSDASADMVDYHISSIFQS+H 
Sbjct: 254 SLGTGVAKNDEKYSAAMSSKWGMLGWIYHRGATPIVDIFSDASADMVDYHISSIFQSEHN 313

Query: 302 HKNYLRIQDDTLSGEVSSVDIATEENLLNLIYVGENLLKKPLSRVNLESGKFEPLDAKGT 361
            +NYLRIQDDTLSG+VSSVDIAT++NLL LI VGE+LLKKPLSRVNLESGKFE LD +GT
Sbjct: 314 DRNYLRIQDDTLSGDVSSVDIATQQNLLKLIEVGESLLKKPLSRVNLESGKFEALDGEGT 373

Query: 362 NEQALAEFAKMLSNERKLRLSP 384
           NE+ALAEFA+MLS+ERKLRLSP
Sbjct: 374 NEKALAEFAQMLSDERKLRLSP 395