BLAST of Cucsa.250410 vs. Swiss-Prot
Match:
GLIP5_ARATH (GDSL esterase/lipase 5 OS=Arabidopsis thaliana GN=GLIP5 PE=2 SV=2)
HSP 1 Score: 359.4 bits (921), Expect = 4.8e-98
Identity = 178/336 (52.98%), Postives = 236/336 (70.24%), Query Frame = 1
Query: 35 FIFGDSILDAGNNNYINTTTNFQANFPPYGLTFFHNPTGRFSDGRLIPDFIAEYAKLPLI 94
F+FGDS LDAGNNNYINTTT QANFPPYG TFF PTGRFSDGRLI DFIAEYA LPLI
Sbjct: 50 FLFGDSFLDAGNNNYINTTTLDQANFPPYGQTFFGLPTGRFSDGRLISDFIAEYANLPLI 109
Query: 95 RPYLDPHNNLY-IHGVNFASGGSGALLESHQGSAITLQTQLTNFIEVGKSLRKKLGDNRA 154
P+L+P N+ ++GVNFAS G+GAL+E+ QGS I L+TQL ++ +V + R G +
Sbjct: 110 PPFLEPGNSQKKLYGVNFASAGAGALVETFQGSVINLRTQLDHYKKVERLWRTNFGKEES 169
Query: 155 QNLLSNSVYLISTGGNDYISLFEGDSTAFQIYTQTQYVNMVIGNLTTVIQEIYKNGGRKF 214
+ +S +VYLIS G NDY S+F + + + +Q+V++VIGNLTT I EIYK GGRKF
Sbjct: 170 KKRISRAVYLISIGSNDYSSIFLTNQSL--PISMSQHVDIVIGNLTTFIHEIYKIGGRKF 229
Query: 215 GLVGVPSLGCMPRLKMLKGEGHGKCVEEASSIVNLHNKLLPIALQNFATQLNGFKYAFAD 274
G + VP LGC P L++L+ + C+ +AS + ++HN+ L L Q+ GFK++ D
Sbjct: 230 GFLNVPDLGCFPALRILQPKNDDSCLRDASRLASMHNRALTNLLFQMQRQVKGFKFSLFD 289
Query: 275 ANNLLLQIIQNPSKYGFKEVETACCGSGEYRGIYSCGGRRGTKEFKLCEDPTKYLFFDSY 334
N L +Q+PSK+GFKE E ACCG+G++RG++SCGG+R KE++LCE+P Y+F+DS
Sbjct: 290 MNKSLRLRMQHPSKFGFKEGEEACCGTGKWRGVFSCGGKRIVKEYQLCENPKDYIFWDSL 349
Query: 335 HPNQKAYEQLARLMWSG----DEQVINPYNLKQLFQ 366
H Q Y Q A L+W+G D V+ PYN+ LFQ
Sbjct: 350 HLTQNTYNQFANLIWNGGHMSDSLVVGPYNINNLFQ 383
BLAST of Cucsa.250410 vs. Swiss-Prot
Match:
GLIP1_ARATH (GDSL esterase/lipase 1 OS=Arabidopsis thaliana GN=GLIP1 PE=1 SV=1)
HSP 1 Score: 326.6 bits (836), Expect = 3.5e-88
Identity = 166/359 (46.24%), Postives = 234/359 (65.18%), Query Frame = 1
Query: 12 VVVLFSSI--VEENIFVFSEQNVGFFIFGDSILDAGNNNYINTTTNFQANFPPYGLTFFH 71
+++ SI ++ N V ++ F+FGDS+ DAGNNNYI+T ++ ++N+ PYG T F
Sbjct: 16 IIISIGSINCIDNNNLVTNQS--ALFVFGDSVFDAGNNNYIDTLSSVRSNYWPYGQTTFK 75
Query: 72 NPTGRFSDGRLIPDFIAEYAKLPLIRPYLDPHN--NLYIHGVNFASGGSGALLESHQGSA 131
+PTGR SDGRLIPDFIAEYA LPLI P L P N + + +GVNFASGG+GAL+ + G
Sbjct: 76 SPTGRVSDGRLIPDFIAEYAWLPLIPPNLQPFNGNSQFAYGVNFASGGAGALVGTFSGLV 135
Query: 132 ITLQTQLTNFIEVGKSLRKKLGDNRAQNLLSNSVYLISTGGNDYISLFEGDSTAFQIYTQ 191
I L+TQL NF +V + LR KLGD + ++S +VYL G NDY F +S+ FQ +
Sbjct: 136 INLRTQLNNFKKVEEMLRSKLGDAEGKRVISRAVYLFHIGLNDYQYPFTTNSSLFQSISN 195
Query: 192 TQYVNMVIGNLTTVIQEIYKNGGRKFGLVGVPSLGCMPRLKMLKGEGHGKCVEEASSIVN 251
+YV+ V+GN+T V +E+Y GGRKFG++ C P ++ C + + ++N
Sbjct: 196 EKYVDYVVGNMTDVFKEVYNLGGRKFGILNTGPYDCAPASLVIDQTKIRSCFQPVTELIN 255
Query: 252 LHNKLLPIALQNFATQLNGFKYAFADANNLLLQIIQNPSKYGFKEVETACCGSGEYRGIY 311
+HN+ L L+ +L+GFKYA D + L + + +PSKYGFKE + ACCGSG RGI
Sbjct: 256 MHNEKLLNGLRRLNHELSGFKYALHDYHTSLSERMNDPSKYGFKEGKKACCGSGPLRGIN 315
Query: 312 SCGGRRG-TKEFKLCEDPTKYLFFDSYHPNQKAYEQLARLMWSGDEQVINPYNLKQLFQ 366
+CGGR G ++ ++LCE+ T YLFFD +H +KA Q+A L+WSG + PYNLK LF+
Sbjct: 316 TCGGRMGLSQSYELCENVTDYLFFDPFHLTEKANRQIAELIWSGPTNITGPYNLKALFE 372
BLAST of Cucsa.250410 vs. Swiss-Prot
Match:
GLIP2_ARATH (GDSL esterase/lipase 2 OS=Arabidopsis thaliana GN=GLIP2 PE=2 SV=1)
HSP 1 Score: 325.1 bits (832), Expect = 1.0e-87
Identity = 164/334 (49.10%), Postives = 222/334 (66.47%), Query Frame = 1
Query: 35 FIFGDSILDAGNNNYINTTTNFQANFPPYGLTFFHNPTGRFSDGRLIPDFIAEYAKLPLI 94
F+FGDS+ DAGNNNYI+T +F++N+ PYG T F PTGR SDGR IPDFIAEYA LPLI
Sbjct: 41 FVFGDSVFDAGNNNYIDTLPSFRSNYWPYGQTTFKFPTGRVSDGRTIPDFIAEYAWLPLI 100
Query: 95 RPYLDPHN--NLYIHGVNFASGGSGALLESHQGSAITLQTQLTNFIEVGKSLRKKLGDNR 154
YL P N N + +GV+FAS G+GAL+ + G I L++QL NF +V K LR LG+ +
Sbjct: 101 PAYLQPSNGKNQFPYGVSFASAGAGALVGTFPGMVINLKSQLNNFKKVEKLLRSTLGEAQ 160
Query: 155 AQNLLSNSVYLISTGGNDYISLFEGDSTAFQIYTQTQYVNMVIGNLTTVIQEIYKNGGRK 214
+ ++S +VYL G NDY F +S+ FQ Q YV+ V+GN T VI+E+YK GGRK
Sbjct: 161 GKMVISRAVYLFHIGVNDYQYPFSTNSSIFQSSPQEIYVDFVVGNTTAVIKEVYKIGGRK 220
Query: 215 FGLVGVPSLGCMPRLKMLKGEGHGKCVEEASSIVNLHNKLLPIALQNFATQLNGFKYAFA 274
FG + + + C P ++ G C + + ++NLHN+ L L+ +L+GFKYA
Sbjct: 221 FGFLNMGAYDCAPASLIIDQTKIGTCFKPVTELINLHNEKLESGLRRLERELSGFKYALH 280
Query: 275 DANNLLLQIIQNPSKYGFKEVETACCGSGEYRGIYSCGGRRG-TKEFKLCEDPTKYLFFD 334
D + L + NPSKYGFKE + ACCG+G RGI +CGGR G ++ ++LCE T YLFFD
Sbjct: 281 DYHTSLSVRMNNPSKYGFKEGKMACCGTGPLRGINTCGGRMGVSQSYELCEKVTDYLFFD 340
Query: 335 SYHPNQKAYEQLARLMWSGDEQVINPYNLKQLFQ 366
+H +KA++Q+A L+WSG V PYNL+ LF+
Sbjct: 341 HFHLTEKAHQQIAELIWSGPTNVTKPYNLQALFE 374
BLAST of Cucsa.250410 vs. Swiss-Prot
Match:
GRIP4_ARATH (GDSL esterase/lipase 4 OS=Arabidopsis thaliana GN=GLIP4 PE=2 SV=2)
HSP 1 Score: 309.7 bits (792), Expect = 4.4e-83
Identity = 162/368 (44.02%), Postives = 223/368 (60.60%), Query Frame = 1
Query: 11 LVVVLFSSIVEENIFVFS------EQNVGFFIFGDSILDAGNNNYINTTTNFQANFPPYG 70
++++LF + +I S F FGDS+ +AGNNNY ++ ++F++NF PYG
Sbjct: 9 IIIILFICTISLSIVSISCKEDLKTNQAALFAFGDSLFEAGNNNYFDSISSFRSNFWPYG 68
Query: 71 LTFFHNPTGRFSDGRLIPDFIAEYAKLPLIRPYLDP--HNNLYIHGVNFASGGSGALLES 130
T F PTGR SDGR++ DFIAEYA LPLI P L P N+ +G+NFA+ +G +
Sbjct: 69 KTTFKFPTGRVSDGRIMIDFIAEYAWLPLIPPNLQPGYSNSQLTYGLNFATTAAGVFAGT 128
Query: 131 HQGSAITLQ----TQLTNFIEVGKSLRKKLGDNRAQNLLSNSVYLISTGGNDYISLFEGD 190
GS L TQL NF V K+LR LGD A+ ++S +VYL G NDY F +
Sbjct: 129 FPGSVTNLSKDLGTQLNNFKNVEKTLRSNLGDAEARRVISKAVYLFHIGANDYQYPFFAN 188
Query: 191 STAFQIYTQTQYVNMVIGNLTTVIQEIYKNGGRKFGLVGVPSLGCMPRLKMLKGEGHGKC 250
++ F T+ ++++ VIGN TTVI+E+YK G RKFG + + GC P ++ G C
Sbjct: 189 TSTFSNTTKERFIDFVIGNTTTVIEELYKLGARKFGFLSLGPFGCTPSALIINSTKIGSC 248
Query: 251 VEEASSIVNLHNKLLPIALQNFATQLNGFKYAFADANNLLLQIIQNPSKYGFKEVETACC 310
E + ++NLHN+ P L+ +L+GFKYA D + L Q I NPS+YGFKE E ACC
Sbjct: 249 FEPVTELINLHNQEFPKVLRRLERRLSGFKYALHDFHTSLSQRINNPSRYGFKEGEMACC 308
Query: 311 GSGEYRGIYSCGGRRGTKE-FKLCEDPTKYLFFDSYHPNQKAYEQLARLMWSGDEQVINP 366
GSG RGI +CG R G + +KLCE+ Y+FFD H + A++Q+A L+WSG V P
Sbjct: 309 GSGPLRGINTCGFRNGPSQGYKLCENADDYVFFDPSHLTETAHQQIAELIWSGPPNVTAP 368
BLAST of Cucsa.250410 vs. Swiss-Prot
Match:
GLIP3_ARATH (GDSL esterase/lipase 3 OS=Arabidopsis thaliana GN=GLIP3 PE=2 SV=2)
HSP 1 Score: 307.4 bits (786), Expect = 2.2e-82
Identity = 159/334 (47.60%), Postives = 212/334 (63.47%), Query Frame = 1
Query: 35 FIFGDSILDAGNNNYINTTTNFQANFPPYGLTFFHNPTGRFSDGRLIPDFIAEYAKLPLI 94
F+FGDS+ DAGNNNYINT ++F++N PYG T F PTGR SDG E A LP I
Sbjct: 37 FVFGDSLFDAGNNNYINTVSSFRSNIWPYGQTNFKFPTGRLSDG-------PEKAWLPSI 96
Query: 95 RPYLDPHN--NLYIHGVNFASGGSGALLESHQGSAITLQTQLTNFIEVGKSLRKKLGDNR 154
P L P+N N + +GV+FAS G+GAL ES G I L TQL NF +V KSLR +LGD
Sbjct: 97 PPNLQPNNGNNQFTYGVSFASAGAGALAESFLGMVINLGTQLNNFKDVEKSLRSELGDAE 156
Query: 155 AQNLLSNSVYLISTGGNDYISLFEGDSTAFQIYTQTQYVNMVIGNLTTVIQEIYKNGGRK 214
+ + S +VYL G NDY F +S+ F+ ++ ++V+ VIGN+T VI+E+YK GGRK
Sbjct: 157 TKRVFSRAVYLFHIGANDYFYPFSANSSTFKSNSKEKFVDFVIGNITFVIEEVYKMGGRK 216
Query: 215 FGLVGVPSLGCMPRLKMLKGEGHGKCVEEASSIVNLHNKLLPIALQNFATQLNGFKYAFA 274
FG + V C P + G C + + ++++HNK P L+ QL+GF+YA
Sbjct: 217 FGFLNVGPYECSPNSLIRDRTKIGSCFKPVAELIDMHNKKFPDVLRRLQRQLSGFRYALH 276
Query: 275 DANNLLLQIIQNPSKYGFKEVETACCGSGEYRGIYSCGGRRGTKE-FKLCEDPTKYLFFD 334
D + L + I +PSKYGFKE + ACCGSG RGI +CG R G + + LCE+ T YLF+D
Sbjct: 277 DYHTSLSERINSPSKYGFKEGKKACCGSGPLRGINTCGNRIGPSQGYGLCENVTDYLFYD 336
Query: 335 SYHPNQKAYEQLARLMWSGDEQVINPYNLKQLFQ 366
S H +KA+ Q+A L+W+G V PYNLK LF+
Sbjct: 337 SSHLTEKAHRQIAELIWNGPPNVTRPYNLKALFE 363
BLAST of Cucsa.250410 vs. TrEMBL
Match:
A0A0A0LMU6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G296120 PE=4 SV=1)
HSP 1 Score: 762.3 bits (1967), Expect = 2.8e-217
Identity = 373/374 (99.73%), Postives = 373/374 (99.73%), Query Frame = 1
Query: 1 MKFSKFQTCLLVVVLFSSIVEENIFVFSEQNVGFFIFGDSILDAGNNNYINTTTNFQANF 60
MKFSKFQTCLLVVVLFSSIVEENIFVFSEQNVGFFIFGDSILDAGNNNYINTTTNFQANF
Sbjct: 1 MKFSKFQTCLLVVVLFSSIVEENIFVFSEQNVGFFIFGDSILDAGNNNYINTTTNFQANF 60
Query: 61 PPYGLTFFHNPTGRFSDGRLIPDFIAEYAKLPLIRPYLDPHNNLYIHGVNFASGGSGALL 120
PPYGLTFFHNPTGRFSDGRLIPDFIAEYAKLPLIRPYLDPHNNLYIHGVNFASGGSGALL
Sbjct: 61 PPYGLTFFHNPTGRFSDGRLIPDFIAEYAKLPLIRPYLDPHNNLYIHGVNFASGGSGALL 120
Query: 121 ESHQGSAITLQTQLTNFIEVGKSLRKKLGDNRAQNLLSNSVYLISTGGNDYISLFEGDST 180
ESHQGSAITLQTQLTNFIEVGKSLRKKLGDNRAQNLLSNSVYLISTGGNDYISLFEGDST
Sbjct: 121 ESHQGSAITLQTQLTNFIEVGKSLRKKLGDNRAQNLLSNSVYLISTGGNDYISLFEGDST 180
Query: 181 AFQIYTQTQYVNMVIGNLTTVIQEIYKNGGRKFGLVGVPSLGCMPRLKMLKGEGHGKCVE 240
AFQIYTQTQYVNMVIGNLTTVIQEIYKNGGRKFGLVGVPSLGCMPRLKMLKGEGHGKCVE
Sbjct: 181 AFQIYTQTQYVNMVIGNLTTVIQEIYKNGGRKFGLVGVPSLGCMPRLKMLKGEGHGKCVE 240
Query: 241 EASSIVNLHNKLLPIALQNFATQLNGFKYAFADANNLLLQIIQNPSKYGFKEVETACCGS 300
EASSIVNLHNKLLPIALQN ATQLNGFKYAFADANNLLLQIIQNPSKYGFKEVETACCGS
Sbjct: 241 EASSIVNLHNKLLPIALQNLATQLNGFKYAFADANNLLLQIIQNPSKYGFKEVETACCGS 300
Query: 301 GEYRGIYSCGGRRGTKEFKLCEDPTKYLFFDSYHPNQKAYEQLARLMWSGDEQVINPYNL 360
GEYRGIYSCGGRRGTKEFKLCEDPTKYLFFDSYHPNQKAYEQLARLMWSGDEQVINPYNL
Sbjct: 301 GEYRGIYSCGGRRGTKEFKLCEDPTKYLFFDSYHPNQKAYEQLARLMWSGDEQVINPYNL 360
Query: 361 KQLFQYGSPSLAYE 375
KQLFQYGSPSLAYE
Sbjct: 361 KQLFQYGSPSLAYE 374
BLAST of Cucsa.250410 vs. TrEMBL
Match:
A0A0A0KCD4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G448130 PE=4 SV=1)
HSP 1 Score: 479.6 bits (1233), Expect = 3.6e-132
Identity = 228/341 (66.86%), Postives = 269/341 (78.89%), Query Frame = 1
Query: 29 EQNVGFFIFGDSILDAGNNNYINTTTNFQANFPPYGLTFFHNPTGRFSDGRLIPDFIAEY 88
E+ + FFIFGDS+ D GNNN+INTT +F+ANF PYG +FF PTGRFSDGRL+PDF+AEY
Sbjct: 32 EKRLAFFIFGDSLFDPGNNNFINTTEDFRANFTPYGESFFKTPTGRFSDGRLVPDFVAEY 91
Query: 89 AKLPLIRPYLDPHNNLYIHGVNFASGGSGALLESHQGSAITLQTQLTNFIEVGKSLRKKL 148
A LPLI YLDPHN YIHGVNFASGG GAL+E+H+G AI ++TQL F +V +S+RKKL
Sbjct: 92 ANLPLIPAYLDPHNKRYIHGVNFASGGGGALVETHRGFAIDIETQLRYFKKVERSIRKKL 151
Query: 149 GDNRAQNLLSNSVYLISTGGNDYISLFEGDSTAFQIYTQTQYVNMVIGNLTTVIQEIYKN 208
GD RA NL SNSVYL S GGNDYI FEG S F YT+ +YVNMVIGN T V++EIYK
Sbjct: 152 GDWRAYNLFSNSVYLFSIGGNDYIVPFEG-SPIFDKYTEREYVNMVIGNATAVLEEIYKK 211
Query: 209 GGRKFGLVGVPSLGCMPRLKML-KGEGHGKCVEEASSIVNLHNKLLPIALQNFATQLNGF 268
GGRKF V VP LGC+P ++++ K GHG C +E S++V LHNKLLP ALQ A +L GF
Sbjct: 212 GGRKFAFVAVPPLGCLPHIRLVKKAGGHGSCWDEPSALVRLHNKLLPGALQKLADKLQGF 271
Query: 269 KYAFADANNLLLQIIQNPSKYGFKEVETACCGSGEYRGIYSCGGRRGTKEFKLCEDPTKY 328
KY D +L I NPSKYGFKE +TACCGSG++RGIYSCGG RG KEF+LCE+P +Y
Sbjct: 272 KYTVGDTYTMLQNRIDNPSKYGFKEEKTACCGSGKFRGIYSCGGMRGVKEFELCENPNEY 331
Query: 329 LFFDSYHPNQKAYEQLARLMWSGDEQVINPYNLKQLFQYGS 369
LFFDSYHPN++AYEQ A+LMWSGD QVINPY+LKQ FQY S
Sbjct: 332 LFFDSYHPNERAYEQFAKLMWSGDSQVINPYSLKQFFQYAS 371
BLAST of Cucsa.250410 vs. TrEMBL
Match:
D7TZL6_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_09s0002g00540 PE=4 SV=1)
HSP 1 Score: 427.9 bits (1099), Expect = 1.2e-116
Identity = 210/368 (57.07%), Postives = 264/368 (71.74%), Query Frame = 1
Query: 6 FQTCLLVVVLFSSIVEENIFVFSEQNVGFFIFGDSILDAGNNNYINTTTNFQANFPPYGL 65
F +CLL+ S + + +V FFIFGDS+LD GNNNYINTTT QANF PYG
Sbjct: 17 FSSCLLIPTSSQSHPHQ-----PQNHVAFFIFGDSLLDPGNNNYINTTTEDQANFRPYGE 76
Query: 66 TFFHNPTGRFSDGRLIPDFIAEYAKLPLIRPYLDPHNNLYIHGVNFASGGSGALLESHQG 125
TFF PTGRFSDGRLIPDFIAEYAKLPLI PYL P N+ + +G NFASGG+GAL E +QG
Sbjct: 77 TFFKYPTGRFSDGRLIPDFIAEYAKLPLIPPYLQPGNHQFTYGANFASGGAGALDEINQG 136
Query: 126 SAITLQTQLTNFIEVGKSLRKKLGDNRAQNLLSNSVYLISTGGNDYISLFEGDSTAFQIY 185
+ L TQL F +V K LR+KLGD ++ LL +VYLIS GGNDYIS + + FQIY
Sbjct: 137 LVVNLNTQLRYFKKVEKHLREKLGDEESKKLLLEAVYLISIGGNDYISPLFRNYSVFQIY 196
Query: 186 TQTQYVNMVIGNLTTVIQEIYKNGGRKFGLVGVPSLGCMPRLKMLKGE--GHGKCVEEAS 245
+ QY++MV+GNLT VIQEIY+ GGRKFG V + LGC+P +K +K + G G+C+EEA+
Sbjct: 197 SHRQYLDMVMGNLTVVIQEIYQKGGRKFGFVNMGPLGCLPAMKAIKLQQGGAGECMEEAT 256
Query: 246 SIVNLHNKLLPIALQNFATQLNGFKYAFADANNLLLQIIQNPSKYGFKEVETACCGSGEY 305
+V LHN++LP LQ ++L GFKY+ D + + NPSKYGFKE + ACCGSG Y
Sbjct: 257 VLVKLHNRVLPEVLQKLGSKLKGFKYSIFDFYTTAKERMDNPSKYGFKEAKIACCGSGPY 316
Query: 306 RGIYSCGGRRGTKEFKLCEDPTKYLFFDSYHPNQKAYEQLARLMWSGDEQVINPYNLKQL 365
RG+YSCGG RGTKE++LC + ++Y+FFDS+HP + Y+QLA L+WSG VI PYNLKQL
Sbjct: 317 RGLYSCGGMRGTKEYELCSNVSEYMFFDSFHPTDRVYQQLAELVWSGTHNVIKPYNLKQL 376
Query: 366 FQYGSPSL 372
F + +
Sbjct: 377 FGHSQEEI 379
BLAST of Cucsa.250410 vs. TrEMBL
Match:
A0A0A0LQ38_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G296110 PE=4 SV=1)
HSP 1 Score: 406.0 bits (1042), Expect = 5.0e-110
Identity = 206/380 (54.21%), Postives = 266/380 (70.00%), Query Frame = 1
Query: 1 MKFSKFQTCLLVVVLFSSIVEENIFVFS-----EQNVGFFIFGDSILDAGNNNYINTTTN 60
M+FS F C + F++++E + + GFFIFGDS +DAGNNNYI TT++
Sbjct: 1 MEFSNFCICCFFI-FFAAVLEAARIQLPGNSSPDTSFGFFIFGDSYVDAGNNNYIITTSD 60
Query: 61 FQANFPPYGLTFFHNP--TGRFSDGRLIPDFIAEYAKLPLIRPYLDPHNNLYIHGVNFAS 120
FQANFPPYG +FF NP TGRF+DGR IPDF+ EYA LPLI PYLDPHN+LY +G NFAS
Sbjct: 61 FQANFPPYGESFFPNPIATGRFTDGRNIPDFLGEYANLPLIPPYLDPHNDLYDYGANFAS 120
Query: 121 GGSGALLESHQGSAITLQTQLTNFIEVGKSLRKKLGDNRAQNLLSNSVYLISTGGNDYIS 180
GG GA+ SHQ AI LQTQ+ F +V KSLR KLG R+++ LSNSV+L + GGNDY++
Sbjct: 121 GGGGAIAMSHQEQAIGLQTQMEFFRKVEKSLRNKLGHARSKSFLSNSVFLFNFGGNDYLN 180
Query: 181 LFEGDSTAFQ-IYTQTQYVNMVIGNLTTVIQEIYKNGGRKFGLVGVPSLGCMPRLKMLKG 240
F+ F+ I Q Q+VNMV+GN+T I+E+Y+ GGRKFG++ VP LG MP ++ K
Sbjct: 181 PFDISYDIFKTIEAQEQFVNMVVGNITIAIKEVYEYGGRKFGVLAVPPLGYMPSSRLKKS 240
Query: 241 EGHGKCVEEASSIVNLHNKLLPIALQNFATQLNGFKYAFADANNLLLQIIQNPSKYGFKE 300
+ EEASSI +HNK L IAL+ + QL GFKY FAD + LLQ IQNP++YGFK
Sbjct: 241 ---AQFFEEASSIARIHNKFLLIALEKLSKQLKGFKYTFADVHTALLQRIQNPTEYGFKV 300
Query: 301 VETACCGSGEYRGIYSCGGRRGTKEFKLCEDPTKYLFFDSYHPNQKAYEQLARLMWSGDE 360
V+TACCGS E+RGIY+CG G+ + C++ ++FFDS+HP QK ++QLA WSGDE
Sbjct: 301 VDTACCGSDEFRGIYNCGREFGSSPYTHCQNLEDHMFFDSFHPTQKVFKQLADEFWSGDE 360
Query: 361 QVINPYNLKQLFQYGSPSLA 373
++ P N KQLF Y +LA
Sbjct: 361 DIVKPVNFKQLFHYDDSTLA 376
BLAST of Cucsa.250410 vs. TrEMBL
Match:
I1NCT6_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_19G262900 PE=4 SV=1)
HSP 1 Score: 401.4 bits (1030), Expect = 1.2e-108
Identity = 189/338 (55.92%), Postives = 250/338 (73.96%), Query Frame = 1
Query: 29 EQNVGFFIFGDSILDAGNNNYINTTTNFQANFPPYGLTFFHNPTGRFSDGRLIPDFIAEY 88
++N F+FGDS+ D GNNNYINTT + QAN+ PYG TFF PTGRFSDGR+IPDFIAEY
Sbjct: 31 KENAALFVFGDSLFDVGNNNYINTTADNQANYSPYGETFFKYPTGRFSDGRVIPDFIAEY 90
Query: 89 AKLPLIRPYLDPHNNLYIHGVNFASGGSGALLESHQGSAITLQTQLTNFIEVGKSLRKKL 148
AKLPLI+PYL P N Y+ GVNFASGG+GAL+E+HQG I L+TQL+ F +V K LR+ L
Sbjct: 91 AKLPLIQPYLFPGNQQYVDGVNFASGGAGALVETHQGLVIDLKTQLSYFKKVSKVLRQDL 150
Query: 149 GDNRAQNLLSNSVYLISTGGNDY-ISLFEGDSTAFQIYTQTQYVNMVIGNLTTVIQEIYK 208
GD LL+ +VYLIS GGNDY ISL E S+ +T +Y++MV+GNLTTVI+ I+K
Sbjct: 151 GDAETTTLLAKAVYLISIGGNDYEISLSENSSST---HTTEKYIDMVVGNLTTVIKGIHK 210
Query: 209 NGGRKFGLVGVPSLGCMPRLKMLKGEGHGKCVEEASSIVNLHNKLLPIALQNFATQLNGF 268
GGRKFG+ +P++GC+P +K L G CVEEAS++ LHN +L + L+ QL GF
Sbjct: 211 TGGRKFGVFNLPAVGCVPFVKALVNGSKGSCVEEASALAKLHNSVLSVELEKLKKQLKGF 270
Query: 269 KYAFADANNLLLQIIQNPSKYGFKEVETACCGSGEYRGIYSCGGRRGTKEFKLCEDPTKY 328
KY++ + NL +I NPSKYGFKE ACCGSG Y+G YSCGG+R K++ LCE+P++Y
Sbjct: 271 KYSYVNYFNLTFDVINNPSKYGFKEGSVACCGSGPYKGYYSCGGKRAVKDYDLCENPSEY 330
Query: 329 LFFDSYHPNQKAYEQLARLMWSGDEQVINPYNLKQLFQ 366
+ FDS HP + A++ +++L+WSG++ + Y+LK LF+
Sbjct: 331 VLFDSLHPTEMAHQIVSQLIWSGNQTIAGSYSLKTLFE 365
BLAST of Cucsa.250410 vs. TAIR10
Match:
AT1G53920.1 (AT1G53920.1 GDSL-motif lipase 5)
HSP 1 Score: 359.4 bits (921), Expect = 2.7e-99
Identity = 178/336 (52.98%), Postives = 236/336 (70.24%), Query Frame = 1
Query: 35 FIFGDSILDAGNNNYINTTTNFQANFPPYGLTFFHNPTGRFSDGRLIPDFIAEYAKLPLI 94
F+FGDS LDAGNNNYINTTT QANFPPYG TFF PTGRFSDGRLI DFIAEYA LPLI
Sbjct: 50 FLFGDSFLDAGNNNYINTTTLDQANFPPYGQTFFGLPTGRFSDGRLISDFIAEYANLPLI 109
Query: 95 RPYLDPHNNLY-IHGVNFASGGSGALLESHQGSAITLQTQLTNFIEVGKSLRKKLGDNRA 154
P+L+P N+ ++GVNFAS G+GAL+E+ QGS I L+TQL ++ +V + R G +
Sbjct: 110 PPFLEPGNSQKKLYGVNFASAGAGALVETFQGSVINLRTQLDHYKKVERLWRTNFGKEES 169
Query: 155 QNLLSNSVYLISTGGNDYISLFEGDSTAFQIYTQTQYVNMVIGNLTTVIQEIYKNGGRKF 214
+ +S +VYLIS G NDY S+F + + + +Q+V++VIGNLTT I EIYK GGRKF
Sbjct: 170 KKRISRAVYLISIGSNDYSSIFLTNQSL--PISMSQHVDIVIGNLTTFIHEIYKIGGRKF 229
Query: 215 GLVGVPSLGCMPRLKMLKGEGHGKCVEEASSIVNLHNKLLPIALQNFATQLNGFKYAFAD 274
G + VP LGC P L++L+ + C+ +AS + ++HN+ L L Q+ GFK++ D
Sbjct: 230 GFLNVPDLGCFPALRILQPKNDDSCLRDASRLASMHNRALTNLLFQMQRQVKGFKFSLFD 289
Query: 275 ANNLLLQIIQNPSKYGFKEVETACCGSGEYRGIYSCGGRRGTKEFKLCEDPTKYLFFDSY 334
N L +Q+PSK+GFKE E ACCG+G++RG++SCGG+R KE++LCE+P Y+F+DS
Sbjct: 290 MNKSLRLRMQHPSKFGFKEGEEACCGTGKWRGVFSCGGKRIVKEYQLCENPKDYIFWDSL 349
Query: 335 HPNQKAYEQLARLMWSG----DEQVINPYNLKQLFQ 366
H Q Y Q A L+W+G D V+ PYN+ LFQ
Sbjct: 350 HLTQNTYNQFANLIWNGGHMSDSLVVGPYNINNLFQ 383
BLAST of Cucsa.250410 vs. TAIR10
Match:
AT5G40990.1 (AT5G40990.1 GDSL lipase 1)
HSP 1 Score: 326.6 bits (836), Expect = 2.0e-89
Identity = 166/359 (46.24%), Postives = 234/359 (65.18%), Query Frame = 1
Query: 12 VVVLFSSI--VEENIFVFSEQNVGFFIFGDSILDAGNNNYINTTTNFQANFPPYGLTFFH 71
+++ SI ++ N V ++ F+FGDS+ DAGNNNYI+T ++ ++N+ PYG T F
Sbjct: 16 IIISIGSINCIDNNNLVTNQS--ALFVFGDSVFDAGNNNYIDTLSSVRSNYWPYGQTTFK 75
Query: 72 NPTGRFSDGRLIPDFIAEYAKLPLIRPYLDPHN--NLYIHGVNFASGGSGALLESHQGSA 131
+PTGR SDGRLIPDFIAEYA LPLI P L P N + + +GVNFASGG+GAL+ + G
Sbjct: 76 SPTGRVSDGRLIPDFIAEYAWLPLIPPNLQPFNGNSQFAYGVNFASGGAGALVGTFSGLV 135
Query: 132 ITLQTQLTNFIEVGKSLRKKLGDNRAQNLLSNSVYLISTGGNDYISLFEGDSTAFQIYTQ 191
I L+TQL NF +V + LR KLGD + ++S +VYL G NDY F +S+ FQ +
Sbjct: 136 INLRTQLNNFKKVEEMLRSKLGDAEGKRVISRAVYLFHIGLNDYQYPFTTNSSLFQSISN 195
Query: 192 TQYVNMVIGNLTTVIQEIYKNGGRKFGLVGVPSLGCMPRLKMLKGEGHGKCVEEASSIVN 251
+YV+ V+GN+T V +E+Y GGRKFG++ C P ++ C + + ++N
Sbjct: 196 EKYVDYVVGNMTDVFKEVYNLGGRKFGILNTGPYDCAPASLVIDQTKIRSCFQPVTELIN 255
Query: 252 LHNKLLPIALQNFATQLNGFKYAFADANNLLLQIIQNPSKYGFKEVETACCGSGEYRGIY 311
+HN+ L L+ +L+GFKYA D + L + + +PSKYGFKE + ACCGSG RGI
Sbjct: 256 MHNEKLLNGLRRLNHELSGFKYALHDYHTSLSERMNDPSKYGFKEGKKACCGSGPLRGIN 315
Query: 312 SCGGRRG-TKEFKLCEDPTKYLFFDSYHPNQKAYEQLARLMWSGDEQVINPYNLKQLFQ 366
+CGGR G ++ ++LCE+ T YLFFD +H +KA Q+A L+WSG + PYNLK LF+
Sbjct: 316 TCGGRMGLSQSYELCENVTDYLFFDPFHLTEKANRQIAELIWSGPTNITGPYNLKALFE 372
BLAST of Cucsa.250410 vs. TAIR10
Match:
AT1G53940.1 (AT1G53940.1 GDSL-motif lipase 2)
HSP 1 Score: 310.8 bits (795), Expect = 1.1e-84
Identity = 157/319 (49.22%), Postives = 213/319 (66.77%), Query Frame = 1
Query: 35 FIFGDSILDAGNNNYINTTTNFQANFPPYGLTFFHNPTGRFSDGRLIPDFIAEYAKLPLI 94
F+FGDS+ DAGNNNYI+T +F++N+ PYG T F PTGR SDGR IPDFIAEYA LPLI
Sbjct: 41 FVFGDSVFDAGNNNYIDTLPSFRSNYWPYGQTTFKFPTGRVSDGRTIPDFIAEYAWLPLI 100
Query: 95 RPYLDPHN--NLYIHGVNFASGGSGALLESHQGSAITLQTQLTNFIEVGKSLRKKLGDNR 154
YL P N N + +GV+FAS G+GAL+ + G I L++QL NF +V K LR LG+ +
Sbjct: 101 PAYLQPSNGKNQFPYGVSFASAGAGALVGTFPGMVINLKSQLNNFKKVEKLLRSTLGEAQ 160
Query: 155 AQNLLSNSVYLISTGGNDYISLFEGDSTAFQIYTQTQYVNMVIGNLTTVIQEIYKNGGRK 214
+ ++S +VYL G NDY F +S+ FQ Q YV+ V+GN T VI+E+YK GGRK
Sbjct: 161 GKMVISRAVYLFHIGVNDYQYPFSTNSSIFQSSPQEIYVDFVVGNTTAVIKEVYKIGGRK 220
Query: 215 FGLVGVPSLGCMPRLKMLKGEGHGKCVEEASSIVNLHNKLLPIALQNFATQLNGFKYAFA 274
FG + + + C P ++ G C + + ++NLHN+ L L+ +L+GFKYA
Sbjct: 221 FGFLNMGAYDCAPASLIIDQTKIGTCFKPVTELINLHNEKLESGLRRLERELSGFKYALH 280
Query: 275 DANNLLLQIIQNPSKYGFKEVETACCGSGEYRGIYSCGGRRG-TKEFKLCEDPTKYLFFD 334
D + L + NPSKYGFKE + ACCG+G RGI +CGGR G ++ ++LCE T YLFFD
Sbjct: 281 DYHTSLSVRMNNPSKYGFKEGKMACCGTGPLRGINTCGGRMGVSQSYELCEKVTDYLFFD 340
Query: 335 SYHPNQKAYEQLARLMWSG 351
+H +KA++Q+A L+WSG
Sbjct: 341 HFHLTEKAHQQIAELIWSG 359
HSP 2 Score: 47.8 bits (112), Expect = 1.8e-05
Identity = 24/71 (33.80%), Postives = 41/71 (57.75%), Query Frame = 1
Query: 274 ANNLLLQIIQNPSKYGFKEVETACCGSGEYRGIYSCGGRRGTKE--FKLCEDPTKYLFFD 333
A+ + ++I + G ++ CG+G RGI +CGGR + ++LCE+ YLF +
Sbjct: 348 AHQQIAELIWSGPTNGLRKGIRHVCGTGPLRGINTCGGRVSAQVQGYELCENVADYLFLN 407
Query: 334 SYHPNQKAYEQ 343
S+H +KA+ Q
Sbjct: 408 SFHLIEKAHRQ 418
BLAST of Cucsa.250410 vs. TAIR10
Match:
AT3G14225.1 (AT3G14225.1 GDSL-motif lipase 4)
HSP 1 Score: 309.7 bits (792), Expect = 2.5e-84
Identity = 162/368 (44.02%), Postives = 223/368 (60.60%), Query Frame = 1
Query: 11 LVVVLFSSIVEENIFVFS------EQNVGFFIFGDSILDAGNNNYINTTTNFQANFPPYG 70
++++LF + +I S F FGDS+ +AGNNNY ++ ++F++NF PYG
Sbjct: 9 IIIILFICTISLSIVSISCKEDLKTNQAALFAFGDSLFEAGNNNYFDSISSFRSNFWPYG 68
Query: 71 LTFFHNPTGRFSDGRLIPDFIAEYAKLPLIRPYLDP--HNNLYIHGVNFASGGSGALLES 130
T F PTGR SDGR++ DFIAEYA LPLI P L P N+ +G+NFA+ +G +
Sbjct: 69 KTTFKFPTGRVSDGRIMIDFIAEYAWLPLIPPNLQPGYSNSQLTYGLNFATTAAGVFAGT 128
Query: 131 HQGSAITLQ----TQLTNFIEVGKSLRKKLGDNRAQNLLSNSVYLISTGGNDYISLFEGD 190
GS L TQL NF V K+LR LGD A+ ++S +VYL G NDY F +
Sbjct: 129 FPGSVTNLSKDLGTQLNNFKNVEKTLRSNLGDAEARRVISKAVYLFHIGANDYQYPFFAN 188
Query: 191 STAFQIYTQTQYVNMVIGNLTTVIQEIYKNGGRKFGLVGVPSLGCMPRLKMLKGEGHGKC 250
++ F T+ ++++ VIGN TTVI+E+YK G RKFG + + GC P ++ G C
Sbjct: 189 TSTFSNTTKERFIDFVIGNTTTVIEELYKLGARKFGFLSLGPFGCTPSALIINSTKIGSC 248
Query: 251 VEEASSIVNLHNKLLPIALQNFATQLNGFKYAFADANNLLLQIIQNPSKYGFKEVETACC 310
E + ++NLHN+ P L+ +L+GFKYA D + L Q I NPS+YGFKE E ACC
Sbjct: 249 FEPVTELINLHNQEFPKVLRRLERRLSGFKYALHDFHTSLSQRINNPSRYGFKEGEMACC 308
Query: 311 GSGEYRGIYSCGGRRGTKE-FKLCEDPTKYLFFDSYHPNQKAYEQLARLMWSGDEQVINP 366
GSG RGI +CG R G + +KLCE+ Y+FFD H + A++Q+A L+WSG V P
Sbjct: 309 GSGPLRGINTCGFRNGPSQGYKLCENADDYVFFDPSHLTETAHQQIAELIWSGPPNVTAP 368
BLAST of Cucsa.250410 vs. TAIR10
Match:
AT1G53990.1 (AT1G53990.1 GDSL-motif lipase 3)
HSP 1 Score: 307.4 bits (786), Expect = 1.2e-83
Identity = 159/334 (47.60%), Postives = 212/334 (63.47%), Query Frame = 1
Query: 35 FIFGDSILDAGNNNYINTTTNFQANFPPYGLTFFHNPTGRFSDGRLIPDFIAEYAKLPLI 94
F+FGDS+ DAGNNNYINT ++F++N PYG T F PTGR SDG E A LP I
Sbjct: 37 FVFGDSLFDAGNNNYINTVSSFRSNIWPYGQTNFKFPTGRLSDG-------PEKAWLPSI 96
Query: 95 RPYLDPHN--NLYIHGVNFASGGSGALLESHQGSAITLQTQLTNFIEVGKSLRKKLGDNR 154
P L P+N N + +GV+FAS G+GAL ES G I L TQL NF +V KSLR +LGD
Sbjct: 97 PPNLQPNNGNNQFTYGVSFASAGAGALAESFLGMVINLGTQLNNFKDVEKSLRSELGDAE 156
Query: 155 AQNLLSNSVYLISTGGNDYISLFEGDSTAFQIYTQTQYVNMVIGNLTTVIQEIYKNGGRK 214
+ + S +VYL G NDY F +S+ F+ ++ ++V+ VIGN+T VI+E+YK GGRK
Sbjct: 157 TKRVFSRAVYLFHIGANDYFYPFSANSSTFKSNSKEKFVDFVIGNITFVIEEVYKMGGRK 216
Query: 215 FGLVGVPSLGCMPRLKMLKGEGHGKCVEEASSIVNLHNKLLPIALQNFATQLNGFKYAFA 274
FG + V C P + G C + + ++++HNK P L+ QL+GF+YA
Sbjct: 217 FGFLNVGPYECSPNSLIRDRTKIGSCFKPVAELIDMHNKKFPDVLRRLQRQLSGFRYALH 276
Query: 275 DANNLLLQIIQNPSKYGFKEVETACCGSGEYRGIYSCGGRRGTKE-FKLCEDPTKYLFFD 334
D + L + I +PSKYGFKE + ACCGSG RGI +CG R G + + LCE+ T YLF+D
Sbjct: 277 DYHTSLSERINSPSKYGFKEGKKACCGSGPLRGINTCGNRIGPSQGYGLCENVTDYLFYD 336
Query: 335 SYHPNQKAYEQLARLMWSGDEQVINPYNLKQLFQ 366
S H +KA+ Q+A L+W+G V PYNLK LF+
Sbjct: 337 SSHLTEKAHRQIAELIWNGPPNVTRPYNLKALFE 363
BLAST of Cucsa.250410 vs. NCBI nr
Match:
gi|449461433|ref|XP_004148446.1| (PREDICTED: GDSL esterase/lipase 5-like [Cucumis sativus])
HSP 1 Score: 762.3 bits (1967), Expect = 4.0e-217
Identity = 373/374 (99.73%), Postives = 373/374 (99.73%), Query Frame = 1
Query: 1 MKFSKFQTCLLVVVLFSSIVEENIFVFSEQNVGFFIFGDSILDAGNNNYINTTTNFQANF 60
MKFSKFQTCLLVVVLFSSIVEENIFVFSEQNVGFFIFGDSILDAGNNNYINTTTNFQANF
Sbjct: 1 MKFSKFQTCLLVVVLFSSIVEENIFVFSEQNVGFFIFGDSILDAGNNNYINTTTNFQANF 60
Query: 61 PPYGLTFFHNPTGRFSDGRLIPDFIAEYAKLPLIRPYLDPHNNLYIHGVNFASGGSGALL 120
PPYGLTFFHNPTGRFSDGRLIPDFIAEYAKLPLIRPYLDPHNNLYIHGVNFASGGSGALL
Sbjct: 61 PPYGLTFFHNPTGRFSDGRLIPDFIAEYAKLPLIRPYLDPHNNLYIHGVNFASGGSGALL 120
Query: 121 ESHQGSAITLQTQLTNFIEVGKSLRKKLGDNRAQNLLSNSVYLISTGGNDYISLFEGDST 180
ESHQGSAITLQTQLTNFIEVGKSLRKKLGDNRAQNLLSNSVYLISTGGNDYISLFEGDST
Sbjct: 121 ESHQGSAITLQTQLTNFIEVGKSLRKKLGDNRAQNLLSNSVYLISTGGNDYISLFEGDST 180
Query: 181 AFQIYTQTQYVNMVIGNLTTVIQEIYKNGGRKFGLVGVPSLGCMPRLKMLKGEGHGKCVE 240
AFQIYTQTQYVNMVIGNLTTVIQEIYKNGGRKFGLVGVPSLGCMPRLKMLKGEGHGKCVE
Sbjct: 181 AFQIYTQTQYVNMVIGNLTTVIQEIYKNGGRKFGLVGVPSLGCMPRLKMLKGEGHGKCVE 240
Query: 241 EASSIVNLHNKLLPIALQNFATQLNGFKYAFADANNLLLQIIQNPSKYGFKEVETACCGS 300
EASSIVNLHNKLLPIALQN ATQLNGFKYAFADANNLLLQIIQNPSKYGFKEVETACCGS
Sbjct: 241 EASSIVNLHNKLLPIALQNLATQLNGFKYAFADANNLLLQIIQNPSKYGFKEVETACCGS 300
Query: 301 GEYRGIYSCGGRRGTKEFKLCEDPTKYLFFDSYHPNQKAYEQLARLMWSGDEQVINPYNL 360
GEYRGIYSCGGRRGTKEFKLCEDPTKYLFFDSYHPNQKAYEQLARLMWSGDEQVINPYNL
Sbjct: 301 GEYRGIYSCGGRRGTKEFKLCEDPTKYLFFDSYHPNQKAYEQLARLMWSGDEQVINPYNL 360
Query: 361 KQLFQYGSPSLAYE 375
KQLFQYGSPSLAYE
Sbjct: 361 KQLFQYGSPSLAYE 374
BLAST of Cucsa.250410 vs. NCBI nr
Match:
gi|659116208|ref|XP_008457963.1| (PREDICTED: uncharacterized protein LOC103497528 [Cucumis melo])
HSP 1 Score: 734.9 bits (1896), Expect = 6.8e-209
Identity = 359/374 (95.99%), Postives = 363/374 (97.06%), Query Frame = 1
Query: 1 MKFSKFQTCLLVVVLFSSIVEENIFVFSEQNVGFFIFGDSILDAGNNNYINTTTNFQANF 60
MK SKFQTCLLVVVLFSS VEENIFVFSEQNVGFFIFGDSILDAGNNNYINTTT FQANF
Sbjct: 423 MKISKFQTCLLVVVLFSSSVEENIFVFSEQNVGFFIFGDSILDAGNNNYINTTTKFQANF 482
Query: 61 PPYGLTFFHNPTGRFSDGRLIPDFIAEYAKLPLIRPYLDPHNNLYIHGVNFASGGSGALL 120
PPYGLTFFHNPTGRFSDGRLIPDFIAEYAKLPLIRPYLDPHNNLYIHGVNFASGGSGALL
Sbjct: 483 PPYGLTFFHNPTGRFSDGRLIPDFIAEYAKLPLIRPYLDPHNNLYIHGVNFASGGSGALL 542
Query: 121 ESHQGSAITLQTQLTNFIEVGKSLRKKLGDNRAQNLLSNSVYLISTGGNDYISLFEGDST 180
ESHQGSAITLQTQL NFIEVGKSLRKKLGDNRAQNLLSNSVYLISTGGNDYISLFEGDST
Sbjct: 543 ESHQGSAITLQTQLKNFIEVGKSLRKKLGDNRAQNLLSNSVYLISTGGNDYISLFEGDST 602
Query: 181 AFQIYTQTQYVNMVIGNLTTVIQEIYKNGGRKFGLVGVPSLGCMPRLKMLKGEGHGKCVE 240
AFQIYTQTQYVNMVIGNLT VIQEIYKNGGRKFG VGVPSLGCMPRLKMLKGEGHGKCVE
Sbjct: 603 AFQIYTQTQYVNMVIGNLTAVIQEIYKNGGRKFGFVGVPSLGCMPRLKMLKGEGHGKCVE 662
Query: 241 EASSIVNLHNKLLPIALQNFATQLNGFKYAFADANNLLLQIIQNPSKYGFKEVETACCGS 300
EASSIVNLHNKLLPIALQ+ ATQLNGFKYAFADANNLLLQ IQNPSKYGFKEVETACCGS
Sbjct: 663 EASSIVNLHNKLLPIALQSLATQLNGFKYAFADANNLLLQRIQNPSKYGFKEVETACCGS 722
Query: 301 GEYRGIYSCGGRRGTKEFKLCEDPTKYLFFDSYHPNQKAYEQLARLMWSGDEQVINPYNL 360
GEYRGIYSCGG+RG KEFK+CEDPTKYLFFDSYHPNQKAYEQLARLMWSGDEQVI PYNL
Sbjct: 723 GEYRGIYSCGGKRGEKEFKICEDPTKYLFFDSYHPNQKAYEQLARLMWSGDEQVIKPYNL 782
Query: 361 KQLFQYGSPSLAYE 375
KQLFQYGSP LAY+
Sbjct: 783 KQLFQYGSPLLAYQ 796
BLAST of Cucsa.250410 vs. NCBI nr
Match:
gi|659116208|ref|XP_008457963.1| (PREDICTED: uncharacterized protein LOC103497528 [Cucumis melo])
HSP 1 Score: 402.1 bits (1032), Expect = 1.0e-108
Identity = 208/382 (54.45%), Postives = 266/382 (69.63%), Query Frame = 1
Query: 1 MKFSKFQTCLLVVVLFSSIVEENIFV------FSEQNVGFFIFGDSILDAGNNNYINTTT 60
MKFS F C+ ++F + V E + S+ GFFIFGDS +DAGNNNYI TT+
Sbjct: 1 MKFSNF--CISFFIIFFAAVSEAASIQLPGNSLSDTRFGFFIFGDSYVDAGNNNYIITTS 60
Query: 61 NFQANFPPYGLTFFHN--PTGRFSDGRLIPDFIAEYAKLPLIRPYLDPHNNLYIHGVNFA 120
+FQANFPPYG +FF N TGRF+DGR IPDF+ EYA LPLI PYLDPHN+LY +GVNFA
Sbjct: 61 DFQANFPPYGESFFPNRIATGRFTDGRNIPDFLGEYANLPLIPPYLDPHNDLYDYGVNFA 120
Query: 121 SGGSGALLESHQGSAITLQTQLTNFIEVGKSLRKKLGDNRAQNLLSNSVYLISTGGNDYI 180
SGG GAL SHQ AI LQTQ+ F +V KSLR KLG R+++ LSNSV+L + GGNDY+
Sbjct: 121 SGGGGALALSHQEQAIGLQTQMVFFRKVVKSLRNKLGHARSRSFLSNSVFLFNFGGNDYL 180
Query: 181 SLFEGDSTAFQ-IYTQTQYVNMVIGNLTTVIQEIYKNGGRKFGLVGVPSLGCMPRLKMLK 240
+ F+ F+ I Q QYVNMV+GN+T I+E+YK GGRKFG++ VP LG MP ++ K
Sbjct: 181 NPFDISYDIFKTIEAQEQYVNMVVGNITIAIKEVYKYGGRKFGVLAVPPLGYMPSSRLKK 240
Query: 241 GEGHGKCVEEASSIVNLHNKLLPIALQNFATQLNGFKYAFADANNLLLQIIQNPSKYGFK 300
+ EE+SSI +HNK + IAL+ + QL GFKY FAD + LLQ IQNP+KYGFK
Sbjct: 241 S---AQFFEESSSIARIHNKFILIALEKLSKQLKGFKYTFADVHTALLQRIQNPTKYGFK 300
Query: 301 EVETACCGSGEYRGIYSCGGRR-GTKEFKLCEDPTKYLFFDSYHPNQKAYEQLARLMWSG 360
V+TACCGS E+RG+Y+CG + G+ + C++ ++FFDS+HP QK ++QLA WSG
Sbjct: 301 VVDTACCGSDEFRGVYNCGRKDFGSLPYTHCQNLEDHMFFDSFHPTQKVFKQLADEFWSG 360
Query: 361 DEQVINPYNLKQLFQYGSPSLA 373
D ++ P N KQLF +LA
Sbjct: 361 DVDIVKPLNFKQLFSCDDSTLA 377
HSP 2 Score: 483.4 bits (1243), Expect = 3.6e-133
Identity = 226/339 (66.67%), Postives = 270/339 (79.65%), Query Frame = 1
Query: 28 SEQNVGFFIFGDSILDAGNNNYINTTTNFQANFPPYGLTFFHNPTGRFSDGRLIPDFIAE 87
SE+ + FFIFGDS D GNNN+INTT +F+ANF PYG +FF PTGRFSD RL+PDF+AE
Sbjct: 31 SEKRLAFFIFGDSFFDPGNNNFINTTEDFRANFTPYGKSFFRTPTGRFSDSRLVPDFVAE 90
Query: 88 YAKLPLIRPYLDPHNNLYIHGVNFASGGSGALLESHQGSAITLQTQLTNFIEVGKSLRKK 147
YA LPLI YLDP N YIHGVNFASGG GAL+E+H+G AI ++TQL F +V +S+RKK
Sbjct: 91 YANLPLIPAYLDPRNKRYIHGVNFASGGGGALVETHRGFAIDIETQLRYFKKVERSIRKK 150
Query: 148 LGDNRAQNLLSNSVYLISTGGNDYISLFEGDSTAFQIYTQTQYVNMVIGNLTTVIQEIYK 207
LGD+RA NL SNSVYL S GGNDYI FEG S F+ YT+T+YVNMVIGN+T V++EIYK
Sbjct: 151 LGDSRAYNLFSNSVYLFSIGGNDYIVPFEG-SPVFEKYTETEYVNMVIGNVTAVLEEIYK 210
Query: 208 NGGRKFGLVGVPSLGCMPRLKMLKGEGHGKCVEEASSIVNLHNKLLPIALQNFATQLNGF 267
GGRKF V VP LGC+P ++++K +GHG C++EAS++V LHNKLL ALQ A +L+GF
Sbjct: 211 KGGRKFAFVAVPPLGCLPHIRLMKKDGHGSCLDEASALVRLHNKLLHAALQKLADKLHGF 270
Query: 268 KYAFADANNLLLQIIQNPSKYGFKEVETACCGSGEYRGIYSCGGRRGTKEFKLCEDPTKY 327
KY D +L I NPSKYG KE + ACCGSG+ RGIYSCGG RG KEF+LCE+P +Y
Sbjct: 271 KYTIGDTYTMLQNRIDNPSKYGLKEGKKACCGSGKLRGIYSCGGMRGVKEFELCENPNEY 330
Query: 328 LFFDSYHPNQKAYEQLARLMWSGDEQVINPYNLKQLFQY 367
LFFDSYHPN++AYEQ A+LMWSGD QVINPYNLKQ FQY
Sbjct: 331 LFFDSYHPNERAYEQFAKLMWSGDSQVINPYNLKQFFQY 368
BLAST of Cucsa.250410 vs. NCBI nr
Match:
gi|449447944|ref|XP_004141726.1| (PREDICTED: GDSL esterase/lipase 1-like [Cucumis sativus])
HSP 1 Score: 479.6 bits (1233), Expect = 5.1e-132
Identity = 228/341 (66.86%), Postives = 269/341 (78.89%), Query Frame = 1
Query: 29 EQNVGFFIFGDSILDAGNNNYINTTTNFQANFPPYGLTFFHNPTGRFSDGRLIPDFIAEY 88
E+ + FFIFGDS+ D GNNN+INTT +F+ANF PYG +FF PTGRFSDGRL+PDF+AEY
Sbjct: 32 EKRLAFFIFGDSLFDPGNNNFINTTEDFRANFTPYGESFFKTPTGRFSDGRLVPDFVAEY 91
Query: 89 AKLPLIRPYLDPHNNLYIHGVNFASGGSGALLESHQGSAITLQTQLTNFIEVGKSLRKKL 148
A LPLI YLDPHN YIHGVNFASGG GAL+E+H+G AI ++TQL F +V +S+RKKL
Sbjct: 92 ANLPLIPAYLDPHNKRYIHGVNFASGGGGALVETHRGFAIDIETQLRYFKKVERSIRKKL 151
Query: 149 GDNRAQNLLSNSVYLISTGGNDYISLFEGDSTAFQIYTQTQYVNMVIGNLTTVIQEIYKN 208
GD RA NL SNSVYL S GGNDYI FEG S F YT+ +YVNMVIGN T V++EIYK
Sbjct: 152 GDWRAYNLFSNSVYLFSIGGNDYIVPFEG-SPIFDKYTEREYVNMVIGNATAVLEEIYKK 211
Query: 209 GGRKFGLVGVPSLGCMPRLKML-KGEGHGKCVEEASSIVNLHNKLLPIALQNFATQLNGF 268
GGRKF V VP LGC+P ++++ K GHG C +E S++V LHNKLLP ALQ A +L GF
Sbjct: 212 GGRKFAFVAVPPLGCLPHIRLVKKAGGHGSCWDEPSALVRLHNKLLPGALQKLADKLQGF 271
Query: 269 KYAFADANNLLLQIIQNPSKYGFKEVETACCGSGEYRGIYSCGGRRGTKEFKLCEDPTKY 328
KY D +L I NPSKYGFKE +TACCGSG++RGIYSCGG RG KEF+LCE+P +Y
Sbjct: 272 KYTVGDTYTMLQNRIDNPSKYGFKEEKTACCGSGKFRGIYSCGGMRGVKEFELCENPNEY 331
Query: 329 LFFDSYHPNQKAYEQLARLMWSGDEQVINPYNLKQLFQYGS 369
LFFDSYHPN++AYEQ A+LMWSGD QVINPY+LKQ FQY S
Sbjct: 332 LFFDSYHPNERAYEQFAKLMWSGDSQVINPYSLKQFFQYAS 371
BLAST of Cucsa.250410 vs. NCBI nr
Match:
gi|659124605|ref|XP_008462251.1| (PREDICTED: GDSL esterase/lipase 5-like isoform X1 [Cucumis melo])
HSP 1 Score: 463.0 bits (1190), Expect = 5.0e-127
Identity = 218/341 (63.93%), Postives = 264/341 (77.42%), Query Frame = 1
Query: 28 SEQNVGFFIFGDSILDAGNNNYINTTTNFQANFPPYGLTFFHNPTGRFSDGRLIPDFIAE 87
S+ + FFIFGDS +D GNNN+INTT F+ANF PYG TFF PTGR SDGR++PDFIAE
Sbjct: 38 SQNRLAFFIFGDSFVDPGNNNFINTTEPFRANFTPYGQTFFKYPTGRVSDGRIMPDFIAE 97
Query: 88 YAKLPLIRPYLDPHNNLYIHGVNFASGGSGALLESHQGSAITLQTQLTNFIEVGKSLRKK 147
YA LPLI YLDPHN LYIHG NFASGG+G L+E+H+G AI ++TQL F +V +S+RKK
Sbjct: 98 YANLPLIPAYLDPHNKLYIHGANFASGGAGVLVETHRGLAIGIETQLRYFKKVERSIRKK 157
Query: 148 LGDNRAQNLLSNSVYLISTGGNDYISLFEGDSTAFQIYTQTQYVNMVIGNLTTVIQEIYK 207
LGD+RA +L SNSVY GGNDY FEG S+ + Y++T++ MVIGNLT V++EIYK
Sbjct: 158 LGDSRAYDLFSNSVYFFHIGGNDYKVPFEG-SSVHEKYSETEHAYMVIGNLTAVLEEIYK 217
Query: 208 NGGRKFGLVGVPSLGCMPRLKMLKGEGHGKCVEEASSIVNLHNKLLPIALQNFATQLNGF 267
GGRKF VG+P LGC+P ++L G G C +EAS++ LHNKL PIALQ FA + GF
Sbjct: 218 KGGRKFAFVGIPPLGCLPHTRLLNKNGDGSCWDEASALATLHNKLFPIALQKFADKFPGF 277
Query: 268 KYAFADANNLLLQIIQNPSKYGFKEVETACCGSGEYRGIYSCGGRRGTKEFKLCEDPTKY 327
KY AD +L I NPSKYGFKE + ACCGSG++RGI+SCG RG +EF+LCE+P +Y
Sbjct: 278 KYTVADMYTMLQNRIDNPSKYGFKEGKKACCGSGKFRGIFSCGRMRGEEEFELCENPNEY 337
Query: 328 LFFDSYHPNQKAYEQLARLMWSGDEQVINPYNLKQLFQYGS 369
LFFDSYHPN++AYEQ A+LMWSGD QVINPYNLKQ FQYGS
Sbjct: 338 LFFDSYHPNERAYEQFAKLMWSGDSQVINPYNLKQFFQYGS 377
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| GLIP5_ARATH | 4.8e-98 | 52.98 | GDSL esterase/lipase 5 OS=Arabidopsis thaliana GN=GLIP5 PE=2 SV=2 | [more] |
| GLIP1_ARATH | 3.5e-88 | 46.24 | GDSL esterase/lipase 1 OS=Arabidopsis thaliana GN=GLIP1 PE=1 SV=1 | [more] |
| GLIP2_ARATH | 1.0e-87 | 49.10 | GDSL esterase/lipase 2 OS=Arabidopsis thaliana GN=GLIP2 PE=2 SV=1 | [more] |
| GRIP4_ARATH | 4.4e-83 | 44.02 | GDSL esterase/lipase 4 OS=Arabidopsis thaliana GN=GLIP4 PE=2 SV=2 | [more] |
| GLIP3_ARATH | 2.2e-82 | 47.60 | GDSL esterase/lipase 3 OS=Arabidopsis thaliana GN=GLIP3 PE=2 SV=2 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A0A0LMU6_CUCSA | 2.8e-217 | 99.73 | Uncharacterized protein OS=Cucumis sativus GN=Csa_2G296120 PE=4 SV=1 | [more] |
| A0A0A0KCD4_CUCSA | 3.6e-132 | 66.86 | Uncharacterized protein OS=Cucumis sativus GN=Csa_7G448130 PE=4 SV=1 | [more] |
| D7TZL6_VITVI | 1.2e-116 | 57.07 | Putative uncharacterized protein OS=Vitis vinifera GN=VIT_09s0002g00540 PE=4 SV=... | [more] |
| A0A0A0LQ38_CUCSA | 5.0e-110 | 54.21 | Uncharacterized protein OS=Cucumis sativus GN=Csa_2G296110 PE=4 SV=1 | [more] |
| I1NCT6_SOYBN | 1.2e-108 | 55.92 | Uncharacterized protein OS=Glycine max GN=GLYMA_19G262900 PE=4 SV=1 | [more] |
