Cucsa.248820 (gene) Cucumber (Gy14) v1

Overview
NameCucsa.248820
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionMBOAT (membrane bound O-acyl transferase) family protein
Locationscaffold02208 : 8892 .. 9938 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGCCGAAATTCGAATTTTCCTTCAAACTCTCCTCTTAGTTTCTTCATCTCTCTCTTTCTGTTTCTCAATCAGAAACTCAATCCCAAATGGCTTCCTCAGATTCCTCTTAATTCTCCCCTTCTTCTCCCTCTTCCTCTACATTCCTCTCCAATTCCAAACCATCCATTTACAAGGCTCCATCGGCTTCTTCATCGGTTGGCTCGCAAGTTTCAAGATCCTCCTCTTTGCCTTCGGTAAAGGTCCTCTCTGTTCCGCTGCCGCCTCCTCCTCCCTCCGCCGCTTCCTCGCCATCGGCTCCCTCCCAATCGAAATCCCCCAGGACACCAACCGCTCCCATCCTAATCCCATTCTTCCCCCGACCTACTTTATAAAATTAGGGCTCCTAATTCTCACACTTGTCGCCATCTATTTCAAAAACTACTTACACCCAACCCTATACCTGATCGTCTTCTGTTTCGTAATTTACTTCCTCCTCGAAATTCTCATCGGTGGCGCCGCCGCCTTCGTGAAGGCGACGCTCGGAGTCGAGTTATTGCCGTATTTCGACGAGCCCTATCTGTCCGTTTCGTTACAGGATTTTTGGGGGAAGCGATGGAATTTATTGACTTCTAGAATCCTTCGCCTCGCCGTTTACGATCCATGCCGGAAACTCACTGTTGGAATCGTCGGGAAGAAAGCGTCGGCGATGATGGCGCTGACGGCAACATTCGCAGTCTCCGGTTTGATGCACGAACTCATCTATTTCTACATGGGAAGATTGGCGCCAACTTGGGAGGTGACTTGCTTCTTTTTATTACATGGAGTTAGTCTTTCAGCGGAGATGGCGATGAAGTCAGCGGTGAGGGGTAGGTTGAAGGTGCCGAGAATTATATCGACGGGGTTTACATGGTGGTTTGTGATGACGACGAGTTCTTGGCTCTTTTTCCCGCAGTTTTTTAGGTTAAAACCGGATGTTAGAATGATGGAAGAACACGCAGCTTTGGGCGCGTTTGTGAAGAACGTGACGGTACAGCTCATCACACCCTTTAAATTTTCTTTCTGA

mRNA sequence

ATGGCGGCCGAAATTCGAATTTTCCTTCAAACTCTCCTCTTAGTTTCTTCATCTCTCTCTTTCTGTTTCTCAATCAGAAACTCAATCCCAAATGGCTTCCTCAGATTCCTCTTAATTCTCCCCTTCTTCTCCCTCTTCCTCTACATTCCTCTCCAATTCCAAACCATCCATTTACAAGGCTCCATCGGCTTCTTCATCGGTTGGCTCGCAAGTTTCAAGATCCTCCTCTTTGCCTTCGGTAAAGGTCCTCTCTGTTCCGCTGCCGCCTCCTCCTCCCTCCGCCGCTTCCTCGCCATCGGCTCCCTCCCAATCGAAATCCCCCAGGACACCAACCGCTCCCATCCTAATCCCATTCTTCCCCCGACCTACTTTATAAAATTAGGGCTCCTAATTCTCACACTTGTCGCCATCTATTTCAAAAACTACTTACACCCAACCCTATACCTGATCGTCTTCTGTTTCGTAATTTACTTCCTCCTCGAAATTCTCATCGGTGGCGCCGCCGCCTTCGTGAAGGCGACGCTCGGAGTCGAGTTATTGCCGTATTTCGACGAGCCCTATCTGTCCGTTTCGTTACAGGATTTTTGGGGGAAGCGATGGAATTTATTGACTTCTAGAATCCTTCGCCTCGCCGTTTACGATCCATGCCGGAAACTCACTGTTGGAATCGTCGGGAAGAAAGCGTCGGCGATGATGGCGCTGACGGCAACATTCGCAGTCTCCGGTTTGATGCACGAACTCATCTATTTCTACATGGGAAGATTGGCGCCAACTTGGGAGGTGACTTGCTTCTTTTTATTACATGGAGTTAGTCTTTCAGCGGAGATGGCGATGAAGTCAGCGGTGAGGGGTAGGTTGAAGGTGCCGAGAATTATATCGACGGGGTTTACATGGTGGTTTGTGATGACGACGAGTTCTTGGCTCTTTTTCCCGCAGTTTTTTAGGTTAAAACCGGATGTTAGAATGATGGAAGAACACGCAGCTTTGGGCGCGTTTGTGAAGAACGTGACGGTACAGCTCATCACACCCTTTAAATTTTCTTTCTGA

Coding sequence (CDS)

ATGGCGGCCGAAATTCGAATTTTCCTTCAAACTCTCCTCTTAGTTTCTTCATCTCTCTCTTTCTGTTTCTCAATCAGAAACTCAATCCCAAATGGCTTCCTCAGATTCCTCTTAATTCTCCCCTTCTTCTCCCTCTTCCTCTACATTCCTCTCCAATTCCAAACCATCCATTTACAAGGCTCCATCGGCTTCTTCATCGGTTGGCTCGCAAGTTTCAAGATCCTCCTCTTTGCCTTCGGTAAAGGTCCTCTCTGTTCCGCTGCCGCCTCCTCCTCCCTCCGCCGCTTCCTCGCCATCGGCTCCCTCCCAATCGAAATCCCCCAGGACACCAACCGCTCCCATCCTAATCCCATTCTTCCCCCGACCTACTTTATAAAATTAGGGCTCCTAATTCTCACACTTGTCGCCATCTATTTCAAAAACTACTTACACCCAACCCTATACCTGATCGTCTTCTGTTTCGTAATTTACTTCCTCCTCGAAATTCTCATCGGTGGCGCCGCCGCCTTCGTGAAGGCGACGCTCGGAGTCGAGTTATTGCCGTATTTCGACGAGCCCTATCTGTCCGTTTCGTTACAGGATTTTTGGGGGAAGCGATGGAATTTATTGACTTCTAGAATCCTTCGCCTCGCCGTTTACGATCCATGCCGGAAACTCACTGTTGGAATCGTCGGGAAGAAAGCGTCGGCGATGATGGCGCTGACGGCAACATTCGCAGTCTCCGGTTTGATGCACGAACTCATCTATTTCTACATGGGAAGATTGGCGCCAACTTGGGAGGTGACTTGCTTCTTTTTATTACATGGAGTTAGTCTTTCAGCGGAGATGGCGATGAAGTCAGCGGTGAGGGGTAGGTTGAAGGTGCCGAGAATTATATCGACGGGGTTTACATGGTGGTTTGTGATGACGACGAGTTCTTGGCTCTTTTTCCCGCAGTTTTTTAGGTTAAAACCGGATGTTAGAATGATGGAAGAACACGCAGCTTTGGGCGCGTTTGTGAAGAACGTGACGGTACAGCTCATCACACCCTTTAAATTTTCTTTCTGA

Protein sequence

MAAEIRIFLQTLLLVSSSLSFCFSIRNSIPNGFLRFLLILPFFSLFLYIPLQFQTIHLQGSIGFFIGWLASFKILLFAFGKGPLCSAAASSSLRRFLAIGSLPIEIPQDTNRSHPNPILPPTYFIKLGLLILTLVAIYFKNYLHPTLYLIVFCFVIYFLLEILIGGAAAFVKATLGVELLPYFDEPYLSVSLQDFWGKRWNLLTSRILRLAVYDPCRKLTVGIVGKKASAMMALTATFAVSGLMHELIYFYMGRLAPTWEVTCFFLLHGVSLSAEMAMKSAVRGRLKVPRIISTGFTWWFVMTTSSWLFFPQFFRLKPDVRMMEEHAALGAFVKNVTVQLITPFKFSF*
Homology
[Close]
BLAST of Cucsa.248820 vs. Swiss-Prot
Match: ASAT1_ARATH (Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana GN=ASAT1 PE=1 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 7.5e-69
Identity = 141/338 (41.72%), Postives = 200/338 (59.17%), Query Frame = 1 

Query: 8   FLQTLLLVSSSLSFCFSIRNSIPNGFLRFLLILPFFSLFLYIPLQFQTIHLQGSIGFFIG 67
           F++   LV  SL + F I   +P G  R +L  P F +F  +P    ++HL G   FFI 
Sbjct: 4   FIKAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAFFIA 63

Query: 68  WLASFKILLFAFGKGPLCSAAASSSLRRFLAIGSLPIEI-----PQDTNRSHPNPILPPT 127
           WLA+FK+LLFA G+GPL S     SL  FLA+  LPI+I     P  T+    +   P  
Sbjct: 64  WLANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKIQLSPKPTKTHSHEGSTEGPLI 123

Query: 128 YFIKLGLLILTLVAIYFKNYLHPTLYLIVFCFVIYFLLEILIGGAAAFVKATLGVELLPY 187
           Y IK   ++L + A  +   L   + L ++   IYF LEI++   AA V+A   +EL P 
Sbjct: 124 YTIKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIILAATAAAVRAMSDLELEPQ 183

Query: 188 FDEPYLSVSLQDFWGKRWNLLTSRILRLAVYDPCRKLTVGIVGKKASAMMALTATFAVSG 247
           F++PYL+ SLQDFWG+RWNL+ + ILR  VY+P  +L   ++G   S ++A   TF VSG
Sbjct: 184 FNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQL-FSVLGPNYSQILAAFGTFVVSG 243

Query: 248 LMHELIYFYMGRLAPTWEVTCFFLLHGVSLSAEMAMKSAVRGRLKVPRIISTGFTWWFVM 307
           +MHELI+FYMGRL P W++  FFL++G   + E+A+K  + GR + P+ IS   T  FVM
Sbjct: 244 IMHELIFFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTINGRWRFPKAISQVLTLTFVM 303

Query: 308 TTSSWLFFPQFFRLKPDVRMMEEHAALGAFVKNVTVQL 341
            T+ WLF P+F R     + ++E+AA+GAF   V  +L
Sbjct: 304 VTALWLFLPEFNRCNIVEKALDEYAAIGAFAVEVRRKL 340

[Close]
BLAST of Cucsa.248820 vs. Swiss-Prot
Match: WAXS1_SIMCH (Long-chain-alcohol O-fatty-acyltransferase OS=Simmondsia chinensis PE=1 SV=1)

HSP 1 Score: 231.9 bits (590), Expect = 1.1e-59
Identity = 129/347 (37.18%), Postives = 198/347 (57.06%), Query Frame = 1 

Query: 4   EIRIFLQTLLLVSSSLSFCFSIRNSIPNG-FLRFLLILPFFSLFLYIPLQFQTIHLQGSI 63
           E++ F +  +   ++  +C  +    P+G  LR LL+LP   LF+++PL+  + HL G  
Sbjct: 6   ELKTFSEVWISAIAAACYCRFVPAVAPHGGALRLLLLLPVVLLFIFLPLRLSSFHLGGPT 65

Query: 64  GFFIGWLASFKILLFAFGKGPLCSAAASSSLRRFLAIGSLPIEIPQDTNRSHPNPILPPT 123
             ++ WLA+FK+LLFAF  GPL +   S SL  F++   LPI+   D +  H       +
Sbjct: 66  ALYLVWLANFKLLLFAFHLGPLSNP--SLSLLHFISTTLLPIKFRDDPSNDHEKNKRTLS 125

Query: 124 Y-FIKLGLLILTLV------AIY-FKNYLHPTLYLIVFCFVIYFLLEILIGGAAAFVKAT 183
           + + K+ L +  LV       IY F+  L   +  +++CF  Y   EI +  +A   +AT
Sbjct: 126 FEWRKVVLFVAKLVFFAGILKIYEFRKDLPHFVISVLYCFHFYLGTEITLAASAVIARAT 185

Query: 184 LGVELLPYFDEPYLSVSLQDFWGKRWNLLTSRILRLAVYDPCRKLTVGIVGKKASAMMAL 243
           LG++L P F+EPYL+ SLQDFWG+RWNL+ S IL L  Y P R++    V  +     A+
Sbjct: 186 LGLDLYPQFNEPYLATSLQDFWGRRWNLMVSDILGLTTYQPVRRVLSRWVRLRWEVAGAM 245

Query: 244 TATFAVSGLMHELIYFYMGRLAPTWEVTCFFLLHGVSLSAEMAMKSAVRGRLKVPRIIST 303
              F VSGLMHE+ +FY+ R  P+WEVT FF+LHGV  + EM +K AV G++++ R +S 
Sbjct: 246 LVAFTVSGLMHEVFFFYLTRARPSWEVTGFFVLHGVCTAVEMVVKKAVSGKVRLRREVSG 305

Query: 304 GFTWWFVMTTSSWLFFPQFFRLKPDVRMMEEHAALGAFVKNVTVQLI 342
             T  FVM T  WLF PQ  R   D++ ++E+  +  + +   + L+
Sbjct: 306 ALTVGFVMVTGGWLFLPQLVRHGVDLKTIDEYPVMFNYTQKKLMGLL 350

[Close]
BLAST of Cucsa.248820 vs. Swiss-Prot
Match: WAXS2_ARATH (Probable long-chain-alcohol O-fatty-acyltransferase 2 OS=Arabidopsis thaliana GN=AT2 PE=3 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 3.5e-58
Identity = 132/332 (39.76%), Postives = 190/332 (57.23%), Query Frame = 1 

Query: 1   MAAEIRIFLQTLLLVSSSLSFCFSIRNSIPNGFLRFLLILPFFSLFLYIPLQFQTIHLQG 60
           M  E+R  ++  +    S+S+C+ I + I  G LR L +LP F +FL +PL F ++H   
Sbjct: 1   MEEELRNLIKVWISALISISYCYYISSKISKGVLRLLSLLPIFIIFLLLPLFFSSVHFCV 60

Query: 61  SIGFFIGWLASFKILLFAFGKGPLCSAAASSSLRRFLAIGSLPIEIPQD--TNRSHPNPI 120
             GFF  WLA+FK+ LFAF + PL  +   S+L RF      PI+I ++  +NR H  P+
Sbjct: 61  ISGFFFTWLANFKLFLFAFDQEPL--SPLPSNLTRFFCFACFPIKINKNPSSNRIHNKPM 120

Query: 121 LPPTYFIKLGLLILTLVAIYFKNY---LHPTLYLIVFCFVIYFLLEILIGGAAAFVKATL 180
                  KL L+   L+ +Y  NY   L    +L +F   +Y   E+++    A +   L
Sbjct: 121 SKWVLAFKL-LIFSFLLHVYRNNYDSGLSRFAFLALFTIHVYLEAELILVFVGALMSMLL 180

Query: 181 GVELLPYFDEPYLSVSLQDFWGKRWNLLTSRILRLAVYDPCRKLTVGIVGKKASAMMALT 240
           G E+ P F++PYL+ SLQ+FW +RWNL+   +LR AV+ P ++    ++G   +    + 
Sbjct: 181 GCEMEPVFNDPYLATSLQEFWSRRWNLMVPAVLRPAVHIPVQRFCAPLLGLHRAFYAGML 240

Query: 241 ATFAVSGLMHELIYFYMGRLAPTWEVTCFFLLHGVSLSAEMAMKSAVRGR-LKVPRIIST 300
           ATF VSGLMHELIYFY+ R +PTWEVTCFFLLHGV    E+AMK   R R L  PR   +
Sbjct: 241 ATFIVSGLMHELIYFYVIRKSPTWEVTCFFLLHGVVTCLEIAMK---RMRWLPTPRRAVS 300

Query: 301 GFT-WWFVMTTSSWLFFPQFFRLKPDVRMMEE 326
           G     F++ T+ WLF+PQ  R     R++ E
Sbjct: 301 GLAITVFLLVTAGWLFYPQMLRNDVHKRVISE 326

[Close]
BLAST of Cucsa.248820 vs. Swiss-Prot
Match: WAXS4_ARATH (Probable long-chain-alcohol O-fatty-acyltransferase 4 OS=Arabidopsis thaliana GN=AT4 PE=2 SV=1)

HSP 1 Score: 225.3 bits (573), Expect = 1.0e-57
Identity = 128/337 (37.98%), Postives = 191/337 (56.68%), Query Frame = 1 

Query: 1   MAAEIRIFLQTLLLVSSSLSFCFSIRNSIPNGFLRFLLILPFFSLFLYIPLQFQTIHLQG 60
           M  E+   ++  +    S+S+C+     I +G  R L +LP   LFL +PL   ++H  G
Sbjct: 1   MEEELMSLIKVWVYAIISISYCYYTSTRIKSGVFRLLSVLPVCVLFLVLPLFVSSVHFSG 60

Query: 61  SIGFFIGWLASFKILLFAFGKGPLCSAAASSSLRRFLAIGSLPIEIPQDTNRSHPNPILP 120
           S  FF+ WLA+FK++LF+F +GPL      S+L RF+     PI++ Q+     P   +P
Sbjct: 61  STAFFLSWLANFKLILFSFDQGPLFPVP--SNLSRFVCFTCFPIKLQQNPK---PQNQMP 120

Query: 121 PTYF-IKLGLLILTLVAIYFKNYLHPTLYLIVFCFVIYFLLEILIGGAAAFVKATLGVEL 180
              F +KL    + L    +K ++ PT+ L+++   IY   EIL+      +  +L  +L
Sbjct: 121 KWGFAVKLAFFGVLLHMYEYKQHMSPTVLLVLYSLHIYLEYEILLAPLKVLLSISLWCDL 180

Query: 181 LPYFDEPYLSVSLQDFWGKRWNLLTSRILRLAVYDPCRKLTVGIVGKKASAMMALTATFA 240
            P+F+EPYLS SLQDFWG+RWNL+   ILR AVY P R++    +    +  + + A+F 
Sbjct: 181 EPHFNEPYLSTSLQDFWGRRWNLMVPAILRPAVYLPVRQMAGRKMNSDQALFLGVFASFL 240

Query: 241 VSGLMHELIYFYMGRLAPTWEVTCFFLLHGVSLSAEMAMK-SAVRGRLKVPRIISTGFTW 300
           VSG++HELI+FY  R +PT EVT FF+LHGV  +AE A K + +  R KV +++S   T 
Sbjct: 241 VSGVVHELIFFYFTRESPTGEVTLFFVLHGVCTAAECAAKRTRLVRRWKVSQMVSRLLTV 300

Query: 301 WFVMTTSSWLFFPQFFRLKPDVRMMEEHAALGAFVKN 336
            FV+ T  WLFFP   R     R+ +E      FVK+
Sbjct: 301 GFVVMTGGWLFFPHLARSGMIERLADEAFLFIGFVKH 332

[Close]
BLAST of Cucsa.248820 vs. Swiss-Prot
Match: WAXS3_ARATH (Probable long-chain-alcohol O-fatty-acyltransferase 3 OS=Arabidopsis thaliana GN=AT3 PE=3 SV=1)

HSP 1 Score: 223.8 bits (569), Expect = 3.0e-57
Identity = 126/314 (40.13%), Postives = 183/314 (58.28%), Query Frame = 1 

Query: 1   MAAEIRIFLQTLLLVSSSLSFCFSIRNSIPNGFLRFLLILPFFSLFLYIPLQFQTIHLQG 60
           M  E++ F++  +    S+S+C+ +   I  G  R L +LP  +LFL  PL F  +H  G
Sbjct: 1   MEEELKNFIKLWISAIISISYCYYLSTGIKAGVFRLLSVLPVCALFLVFPLFFSYVHFSG 60

Query: 61  SIGFFIGWLASFKILLFAFGKGPLCSAAASSSLRRFLAIGSLPIEIPQDTN-RSHPNPIL 120
            + FF+ WLA+FK++LF+F +GPL  +    +L RF+ I   PI+  Q+ N +++  PI 
Sbjct: 61  CMAFFLSWLANFKLILFSFDQGPL--SPLPRTLSRFICITCFPIKPQQNPNIQNYKIPIW 120

Query: 121 PPTYFIKLGLLILTLVAIYFKNYLHPTLYLIVFCFVIYFLLEILIGGAAAFVKATLGVEL 180
              + IK+ + ++ L    +K YL P L L+     I+  LEI+     A V  TLG +L
Sbjct: 121 --LFAIKVVIFVVLLQMYEYKQYLSPALLLVFNSLHIFLELEIVFMLVKALVFITLGCDL 180

Query: 181 LPYFDEPYLSVSLQDFWGKRWNLLTSRILRLAVYDPCRKLTVGIVGKKASAMMALTATFA 240
            P  +EPYL+ SLQDFWG+RWNL+   ILR AVY P R++    V    +  + + A F 
Sbjct: 181 EPQSNEPYLATSLQDFWGRRWNLMVPAILRPAVYLPARRMACRKVNSDQAMFLGVFAAFL 240

Query: 241 VSGLMHELIYFYMGRLAPTWEVTCFFLLHGVSLSAEMAMKSA--VRGRLKVPRIISTGFT 300
           VSG +HE+++FY+ R  PT EVT FFLLHGV   AE+A+K +  VR   +V   +S   T
Sbjct: 241 VSGAVHEMLFFYLTREVPTGEVTWFFLLHGVCTVAEVAVKKSTFVRRWWRVSPTVSRLLT 300

Query: 301 WWFVMTTSSWLFFP 312
             FV+ TS W FFP
Sbjct: 301 VGFVVVTSGWFFFP 310

[Close]
BLAST of Cucsa.248820 vs. TrEMBL
Match: A0A0A0LES4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G684670 PE=4 SV=1)

HSP 1 Score: 689.9 bits (1779), Expect = 1.6e-195
Identity = 345/348 (99.14%), Postives = 347/348 (99.71%), Query Frame = 1 

Query: 1   MAAEIRIFLQTLLLVSSSLSFCFSIRNSIPNGFLRFLLILPFFSLFLYIPLQFQTIHLQG 60
           MAAEIRIFLQTLLLVSSSLSFCFSIRNSIPNGFLRFLLILPFFSLFLYIPLQFQTIHLQG
Sbjct: 1   MAAEIRIFLQTLLLVSSSLSFCFSIRNSIPNGFLRFLLILPFFSLFLYIPLQFQTIHLQG 60

Query: 61  SIGFFIGWLASFKILLFAFGKGPLCSAAASSSLRRFLAIGSLPIEIPQDTNRSHPNPILP 120
           SIGFFIGWLASFK+LLFAFGKGPLCSAAASSSLRRFLAIGSLPIEIPQDTNRSHPNPILP
Sbjct: 61  SIGFFIGWLASFKLLLFAFGKGPLCSAAASSSLRRFLAIGSLPIEIPQDTNRSHPNPILP 120

Query: 121 PTYFIKLGLLILTLVAIYFKNYLHPTLYLIVFCFVIYFLLEILIGGAAAFVKATLGVELL 180
           PTYFIKL LLILTLVAIYFKNYLHPTLYLIVFCFVIYFLLEILIGGAAAFVKATLGVELL
Sbjct: 121 PTYFIKLLLLILTLVAIYFKNYLHPTLYLIVFCFVIYFLLEILIGGAAAFVKATLGVELL 180

Query: 181 PYFDEPYLSVSLQDFWGKRWNLLTSRILRLAVYDPCRKLTVGIVGKKASAMMALTATFAV 240
           PYFDEPYLSVSLQDFWGKRWNLLTSRILRLAVYDPCRKLTVGIVGKKASAM+ALTATFAV
Sbjct: 181 PYFDEPYLSVSLQDFWGKRWNLLTSRILRLAVYDPCRKLTVGIVGKKASAMVALTATFAV 240

Query: 241 SGLMHELIYFYMGRLAPTWEVTCFFLLHGVSLSAEMAMKSAVRGRLKVPRIISTGFTWWF 300
           SGLMHELIYFYMGRLAPTWEVTCFFLLHGVSLSAEMAMKSAVRGRLKVPRIISTGFTWWF
Sbjct: 241 SGLMHELIYFYMGRLAPTWEVTCFFLLHGVSLSAEMAMKSAVRGRLKVPRIISTGFTWWF 300

Query: 301 VMTTSSWLFFPQFFRLKPDVRMMEEHAALGAFVKNVTVQLITPFKFSF 349
           VMTTSSWLFFPQFFRLKPDVRMMEEHAALGAFVKNVTVQLITPFKFSF
Sbjct: 301 VMTTSSWLFFPQFFRLKPDVRMMEEHAALGAFVKNVTVQLITPFKFSF 348

[Close]
BLAST of Cucsa.248820 vs. TrEMBL
Match: A0A0A0L9R8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G683670 PE=4 SV=1)

HSP 1 Score: 469.2 bits (1206), Expect = 4.5e-129
Identity = 231/345 (66.96%), Postives = 272/345 (78.84%), Query Frame = 1 

Query: 1   MAAEIRIFLQTLLLVSSSLSFCFSIRNSIPNGFLRFLLILPFFSLFLYIPLQFQTIHLQG 60
           MA+EI   LQTL LVSSSLS CFSIRNSIPNGF +FLLILPFF LFLYIPLQF TIH QG
Sbjct: 1   MASEIHSLLQTLFLVSSSLSLCFSIRNSIPNGFFKFLLILPFFFLFLYIPLQFHTIHFQG 60

Query: 61  SIGFFIGWLASFKILLFAFGKGPLCSAAASSSLRRFLAIGSLPIEIPQDTNRSHPN-PIL 120
            IGFFIGWL SFK+LLFAFGKGPLCSAA SSSL RFLA+GSLPIEIP   ++S PN  ++
Sbjct: 61  PIGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLPRFLAVGSLPIEIPD--HKSPPNHSLI 120

Query: 121 PPTYFIKLGLLILTLVAIYFKNYLHPTLYLIVFCFVIYFLLEILIGGAAAFVKATLGVEL 180
           PP+   KL  LILT++AI FKN+LHP    + +C +IYF LE LIG  A   K  LGVEL
Sbjct: 121 PPS--AKLIFLILTILAINFKNHLHPNAIPLFYCLLIYFFLEFLIGTTATLAKTVLGVEL 180

Query: 181 LPYFDEPYLSVSLQDFWGKRWNLLTSRILRLAVYDPCRKLTVGIVGKKASAMMALTATFA 240
           LPYF+EPY S SLQDFWG+RWNL+TSRILRL +YDPCR LT+G++G++ ++M+A+ ATF 
Sbjct: 181 LPYFNEPYFSDSLQDFWGRRWNLMTSRILRLTIYDPCRNLTIGVIGRRPASMVAVIATFV 240

Query: 241 VSGLMHELIYFYMGRLAPTWEVTCFFLLHGVSLSAEMAMKSAVRGRLKVPRIISTGFTWW 300
           VSGLMHELIYFYMGR+APTWEVTCFF++HGV + AEMA++    GR + PR+     T  
Sbjct: 241 VSGLMHELIYFYMGRMAPTWEVTCFFVVHGVCVVAEMAVRLGAGGRFRAPRVARICLTIM 300

Query: 301 FVMTTSSWLFFPQFFRLKPDVRMMEEHAALGAFVKNVTVQLITPF 345
           FVM T SWLFFPQ  R K DVRM+EE+AA+GAF K +T+  I PF
Sbjct: 301 FVMGTGSWLFFPQCIRAKMDVRMLEEYAAIGAFFKTITLHFIPPF 341

[Close]
BLAST of Cucsa.248820 vs. TrEMBL
Match: A0A061GG81_THECC (Acyl-CoA sterol acyl transferase 1, putative OS=Theobroma cacao GN=TCM_030359 PE=4 SV=1)

HSP 1 Score: 323.9 bits (829), Expect = 2.3e-85
Identity = 170/375 (45.33%), Postives = 233/375 (62.13%), Query Frame = 1 

Query: 2   AAEIRIFLQTLLLVSSSLSFCFSIRNSIPNGFLRFLLILPFFSLFLYIPLQFQTIHLQGS 61
           + E+  F++  L V  SLS+C++I   IP G  R L +LP   L++ +PL   ++HL G+
Sbjct: 3   SGEMNNFMKIWLCVVISLSYCYAIGKIIPKGTARLLCLLPVVCLYVLLPLNLSSLHLGGA 62

Query: 62  IGFFIGWLASFKILLFAFGKGPLCSAAASSSLRRFLAIGSLPIEI-PQDTNRSHPN---- 121
             FFI WL +FK+LLFAFGKGPL   +   SL RFLA+  LPI+I    T +SHPN    
Sbjct: 63  TAFFIAWLGNFKLLLFAFGKGPL---SPPLSLLRFLAVACLPIKILHNPTPKSHPNGLNK 122

Query: 122 ------------------PILPP-------TYFIKLGLLILTLVAIYFKNYLHPTLYLIV 181
                             P+ P         Y IK  LL L L A  +  Y+HPT  L++
Sbjct: 123 ENPSLKKTLDSQNKEIPGPVKPKKGQKSPINYAIKFLLLALLLRAYDYSEYIHPTFMLVL 182

Query: 182 FCFVIYFLLEILIGGAAAFVKATLGVELLPYFDEPYLSVSLQDFWGKRWNLLTSRILRLA 241
           +C  +YF LEI++   AA  +A LG+EL   F+EPYLS SLQDFWG+RWN++ + ILR  
Sbjct: 183 YCGHVYFCLEIILAMVAALARAMLGLELEQQFNEPYLSTSLQDFWGRRWNIMVTSILRPT 242

Query: 242 VYDPCRKLTVGIVGKKASAMMALTATFAVSGLMHELIYFYMGRLAPTWEVTCFFLLHGVS 301
           VY+P   +   +VG+K + + A+  +F VS +MHELI++Y+GR+ PTWE+T FFLLHG  
Sbjct: 243 VYEPVLNMGARLVGRKWAPLPAVFGSFVVSAVMHELIFYYLGRVRPTWEITWFFLLHGGC 302

Query: 302 LSAEMAMKSAVRGRLKVPRIISTGFTWWFVMTTSSWLFFPQFFRLKPDVRMMEEHAALGA 347
           L AE+A+K  +R +L++P  +ST  T  F+M T SWLFFPQ  R+K D R +EE+AALGA
Sbjct: 303 LMAEIALKKTLRDKLRLPTAVSTPLTIGFIMVTGSWLFFPQLLRIKADERALEEYAALGA 362

[Close]
BLAST of Cucsa.248820 vs. TrEMBL
Match: B9HNV0_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0009s05990g PE=4 SV=1)

HSP 1 Score: 322.4 bits (825), Expect = 6.8e-85
Identity = 162/338 (47.93%), Postives = 228/338 (67.46%), Query Frame = 1 

Query: 1   MAAEIRIFLQTLLLVSSSLSFCFSIRNSIPNGFLRFLLILPFFSLFLYIPLQFQTIHLQG 60
           M  EI  F++  + V  SL FC+SIRN +P G  R L +LP   LFLY+PL+  ++HL G
Sbjct: 1   MEGEILNFIRVWISVLVSLCFCYSIRNIVPKGTKRLLSVLPVVCLFLYLPLKISSVHLGG 60

Query: 61  SIGFFIGWLASFKILLFAFGKGPLCSAAASSSLRRFLAIGSLPIEIPQDTNRSHPNPILP 120
           S  FFI WLA+FK+LLFAFGKGPL S+  S SL  F+A+  LPI+I Q  N +       
Sbjct: 61  STAFFIAWLANFKVLLFAFGKGPL-SSDPSISLPLFIALACLPIKIRQKENPAPSRGKEG 120

Query: 121 PTYFIKLGLLILTLVAIY-FKNYLHPTLYLIVFCFVIYFLLEILIGGAAAFVKATLGVEL 180
              +   G+L+  L+  Y + +Y+HP + LI++ F +YFLLEIL+   A   +  LG+EL
Sbjct: 121 LRNYAVKGVLLAMLIRAYDYSDYIHPNVILIMYSFHVYFLLEILLAVGAVLARNFLGLEL 180

Query: 181 LPYFDEPYLSVSLQDFWGKRWNLLTSRILRLAVYDPCRKLTVGIVGKKASAMMALTATFA 240
            P F+EPYL+ SLQDFWG RWNL+ + ILR  VY+P R +   ++G+K + + A+ ATF 
Sbjct: 181 EPQFNEPYLATSLQDFWGCRWNLVVTSILRPTVYEPTRAIGSHLIGRKWAPLPAVFATFV 240

Query: 241 VSGLMHELIYFYMGRLAPTWEVTCFFLLHGVSLSAEMAMKSAVRGRLKVPRIISTGFTWW 300
           VS +MHE+I++Y+GR+ P WE++ FFLLHG  L+AE+A+K  +  R ++P++IST  T  
Sbjct: 241 VSAIMHEIIFYYLGRVRPNWEISWFFLLHGFCLTAEIALKKVLNDRWRLPKMISTMLTVG 300

Query: 301 FVMTTSSWLFFPQFFRLKPDVRMMEEHAALGAFVKNVT 338
           FVM+T  WLFFP+F   K DVR  EE+A +GA++KNV+
Sbjct: 301 FVMSTGFWLFFPKFVEYKVDVRAFEEYAEIGAYMKNVS 337

[Close]
BLAST of Cucsa.248820 vs. TrEMBL
Match: M5X7K4_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa018212mg PE=4 SV=1)

HSP 1 Score: 320.1 bits (819), Expect = 3.4e-84
Identity = 171/363 (47.11%), Postives = 232/363 (63.91%), Query Frame = 1 

Query: 1   MAAEIRIFLQTLLLVSSSLSFCFSIRNSIPNGFLRFLLILPFFSLFLYIPLQFQTIHLQG 60
           M  EI  F++  L V  SL +C++    +P GF R LLI+P  +LFL +PL   TIHL G
Sbjct: 1   MEGEIGNFIKVWLSVFLSLFYCYAFGKFVPKGFPRLLLIIPVVALFLDLPLHLSTIHLGG 60

Query: 61  SIGFFIGWLASFKILLFAFGKGPLCSAAASSSLRRFLAIGSLPIEIPQD----------- 120
           +  FFI WLA+FK+LLFAFGKGPLCS   S SL RF+A+  LPI+I Q+           
Sbjct: 61  TTAFFISWLANFKLLLFAFGKGPLCSDP-SISLPRFVAVACLPIKIQQNPPPKTPQFQTS 120

Query: 121 --------------TNRSHPNPILPPTYFIKLGLLILTLVAIY-FKNYLHPTLYLIVFCF 180
                         T + +P P  P  Y +K GLL+  L+ +Y +K +LHP L  +++CF
Sbjct: 121 NHQNGHNSNTNGHATTKQNPAPKSPLNYALK-GLLLALLIKVYDYKEHLHPNLIWVMYCF 180

Query: 181 VIYFLLEILIGGAAAFVKATLGVELLPYFDEPYLSVSLQDFWGKRWNLLTSRILRLAVYD 240
            IYF LEI++  AAA  +A LG+EL P F+EPYLS SLQDFWG+RWN++ + ILR  VY+
Sbjct: 181 HIYFALEIILLMAAALARALLGLELEPQFNEPYLSSSLQDFWGRRWNIMVTSILRPTVYE 240

Query: 241 PCRKLTVGIVGKKASAMMALTATFAVSGLMHELIYFYMGRLAPTWEVTCFFLLHGVSLSA 300
           P   L+  +VG++ + + A+  TF VS +MHELI++Y+GRL PTWE++ FFLLHG+ L  
Sbjct: 241 PVVALSTRVVGRRWAPLPAVMGTFIVSAIMHELIFYYLGRLPPTWEISWFFLLHGLCLVV 300

Query: 301 EMAMKSAV--RGRLKVPRIISTGFTWWFVMTTSSWLFFPQFFRLKPDVRMMEEHAALGAF 336
           E+A+K A+  R + +VPR++S   T  FV  T  WLFFP   R   DVR + E+AA+GAF
Sbjct: 301 EIAVKKAIAKRKKFQVPRLVSGPLTVAFVTGTVFWLFFPPLLRCNADVRALGEYAAVGAF 360

[Close]
BLAST of Cucsa.248820 vs. TAIR10
Match: AT3G51970.1 (AT3G51970.1 acyl-CoA sterol acyl transferase 1)

HSP 1 Score: 262.3 bits (669), Expect = 4.2e-70
Identity = 141/338 (41.72%), Postives = 200/338 (59.17%), Query Frame = 1 

Query: 8   FLQTLLLVSSSLSFCFSIRNSIPNGFLRFLLILPFFSLFLYIPLQFQTIHLQGSIGFFIG 67
           F++   LV  SL + F I   +P G  R +L  P F +F  +P    ++HL G   FFI 
Sbjct: 4   FIKAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAFFIA 63

Query: 68  WLASFKILLFAFGKGPLCSAAASSSLRRFLAIGSLPIEI-----PQDTNRSHPNPILPPT 127
           WLA+FK+LLFA G+GPL S     SL  FLA+  LPI+I     P  T+    +   P  
Sbjct: 64  WLANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKIQLSPKPTKTHSHEGSTEGPLI 123

Query: 128 YFIKLGLLILTLVAIYFKNYLHPTLYLIVFCFVIYFLLEILIGGAAAFVKATLGVELLPY 187
           Y IK   ++L + A  +   L   + L ++   IYF LEI++   AA V+A   +EL P 
Sbjct: 124 YTIKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIILAATAAAVRAMSDLELEPQ 183

Query: 188 FDEPYLSVSLQDFWGKRWNLLTSRILRLAVYDPCRKLTVGIVGKKASAMMALTATFAVSG 247
           F++PYL+ SLQDFWG+RWNL+ + ILR  VY+P  +L   ++G   S ++A   TF VSG
Sbjct: 184 FNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQL-FSVLGPNYSQILAAFGTFVVSG 243

Query: 248 LMHELIYFYMGRLAPTWEVTCFFLLHGVSLSAEMAMKSAVRGRLKVPRIISTGFTWWFVM 307
           +MHELI+FYMGRL P W++  FFL++G   + E+A+K  + GR + P+ IS   T  FVM
Sbjct: 244 IMHELIFFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTINGRWRFPKAISQVLTLTFVM 303

Query: 308 TTSSWLFFPQFFRLKPDVRMMEEHAALGAFVKNVTVQL 341
            T+ WLF P+F R     + ++E+AA+GAF   V  +L
Sbjct: 304 VTALWLFLPEFNRCNIVEKALDEYAAIGAFAVEVRRKL 340

[Close]
BLAST of Cucsa.248820 vs. TAIR10
Match: AT5G55370.1 (AT5G55370.1 MBOAT (membrane bound O-acyl transferase) family protein)

HSP 1 Score: 226.9 bits (577), Expect = 2.0e-59
Identity = 132/332 (39.76%), Postives = 190/332 (57.23%), Query Frame = 1 

Query: 1   MAAEIRIFLQTLLLVSSSLSFCFSIRNSIPNGFLRFLLILPFFSLFLYIPLQFQTIHLQG 60
           M  E+R  ++  +    S+S+C+ I + I  G LR L +LP F +FL +PL F ++H   
Sbjct: 1   MEEELRNLIKVWISALISISYCYYISSKISKGVLRLLSLLPIFIIFLLLPLFFSSVHFCV 60

Query: 61  SIGFFIGWLASFKILLFAFGKGPLCSAAASSSLRRFLAIGSLPIEIPQD--TNRSHPNPI 120
             GFF  WLA+FK+ LFAF + PL  +   S+L RF      PI+I ++  +NR H  P+
Sbjct: 61  ISGFFFTWLANFKLFLFAFDQEPL--SPLPSNLTRFFCFACFPIKINKNPSSNRIHNKPM 120

Query: 121 LPPTYFIKLGLLILTLVAIYFKNY---LHPTLYLIVFCFVIYFLLEILIGGAAAFVKATL 180
                  KL L+   L+ +Y  NY   L    +L +F   +Y   E+++    A +   L
Sbjct: 121 SKWVLAFKL-LIFSFLLHVYRNNYDSGLSRFAFLALFTIHVYLEAELILVFVGALMSMLL 180

Query: 181 GVELLPYFDEPYLSVSLQDFWGKRWNLLTSRILRLAVYDPCRKLTVGIVGKKASAMMALT 240
           G E+ P F++PYL+ SLQ+FW +RWNL+   +LR AV+ P ++    ++G   +    + 
Sbjct: 181 GCEMEPVFNDPYLATSLQEFWSRRWNLMVPAVLRPAVHIPVQRFCAPLLGLHRAFYAGML 240

Query: 241 ATFAVSGLMHELIYFYMGRLAPTWEVTCFFLLHGVSLSAEMAMKSAVRGR-LKVPRIIST 300
           ATF VSGLMHELIYFY+ R +PTWEVTCFFLLHGV    E+AMK   R R L  PR   +
Sbjct: 241 ATFIVSGLMHELIYFYVIRKSPTWEVTCFFLLHGVVTCLEIAMK---RMRWLPTPRRAVS 300

Query: 301 GFT-WWFVMTTSSWLFFPQFFRLKPDVRMMEE 326
           G     F++ T+ WLF+PQ  R     R++ E
Sbjct: 301 GLAITVFLLVTAGWLFYPQMLRNDVHKRVISE 326

[Close]
BLAST of Cucsa.248820 vs. TAIR10
Match: AT5G55350.1 (AT5G55350.1 MBOAT (membrane bound O-acyl transferase) family protein)

HSP 1 Score: 225.3 bits (573), Expect = 5.7e-59
Identity = 128/337 (37.98%), Postives = 191/337 (56.68%), Query Frame = 1 

Query: 1   MAAEIRIFLQTLLLVSSSLSFCFSIRNSIPNGFLRFLLILPFFSLFLYIPLQFQTIHLQG 60
           M  E+   ++  +    S+S+C+     I +G  R L +LP   LFL +PL   ++H  G
Sbjct: 1   MEEELMSLIKVWVYAIISISYCYYTSTRIKSGVFRLLSVLPVCVLFLVLPLFVSSVHFSG 60

Query: 61  SIGFFIGWLASFKILLFAFGKGPLCSAAASSSLRRFLAIGSLPIEIPQDTNRSHPNPILP 120
           S  FF+ WLA+FK++LF+F +GPL      S+L RF+     PI++ Q+     P   +P
Sbjct: 61  STAFFLSWLANFKLILFSFDQGPLFPVP--SNLSRFVCFTCFPIKLQQNPK---PQNQMP 120

Query: 121 PTYF-IKLGLLILTLVAIYFKNYLHPTLYLIVFCFVIYFLLEILIGGAAAFVKATLGVEL 180
              F +KL    + L    +K ++ PT+ L+++   IY   EIL+      +  +L  +L
Sbjct: 121 KWGFAVKLAFFGVLLHMYEYKQHMSPTVLLVLYSLHIYLEYEILLAPLKVLLSISLWCDL 180

Query: 181 LPYFDEPYLSVSLQDFWGKRWNLLTSRILRLAVYDPCRKLTVGIVGKKASAMMALTATFA 240
            P+F+EPYLS SLQDFWG+RWNL+   ILR AVY P R++    +    +  + + A+F 
Sbjct: 181 EPHFNEPYLSTSLQDFWGRRWNLMVPAILRPAVYLPVRQMAGRKMNSDQALFLGVFASFL 240

Query: 241 VSGLMHELIYFYMGRLAPTWEVTCFFLLHGVSLSAEMAMK-SAVRGRLKVPRIISTGFTW 300
           VSG++HELI+FY  R +PT EVT FF+LHGV  +AE A K + +  R KV +++S   T 
Sbjct: 241 VSGVVHELIFFYFTRESPTGEVTLFFVLHGVCTAAECAAKRTRLVRRWKVSQMVSRLLTV 300

Query: 301 WFVMTTSSWLFFPQFFRLKPDVRMMEEHAALGAFVKN 336
            FV+ T  WLFFP   R     R+ +E      FVK+
Sbjct: 301 GFVVMTGGWLFFPHLARSGMIERLADEAFLFIGFVKH 332

[Close]
BLAST of Cucsa.248820 vs. TAIR10
Match: AT5G55360.1 (AT5G55360.1 MBOAT (membrane bound O-acyl transferase) family protein)

HSP 1 Score: 223.8 bits (569), Expect = 1.7e-58
Identity = 126/314 (40.13%), Postives = 183/314 (58.28%), Query Frame = 1 

Query: 1   MAAEIRIFLQTLLLVSSSLSFCFSIRNSIPNGFLRFLLILPFFSLFLYIPLQFQTIHLQG 60
           M  E++ F++  +    S+S+C+ +   I  G  R L +LP  +LFL  PL F  +H  G
Sbjct: 1   MEEELKNFIKLWISAIISISYCYYLSTGIKAGVFRLLSVLPVCALFLVFPLFFSYVHFSG 60

Query: 61  SIGFFIGWLASFKILLFAFGKGPLCSAAASSSLRRFLAIGSLPIEIPQDTN-RSHPNPIL 120
            + FF+ WLA+FK++LF+F +GPL  +    +L RF+ I   PI+  Q+ N +++  PI 
Sbjct: 61  CMAFFLSWLANFKLILFSFDQGPL--SPLPRTLSRFICITCFPIKPQQNPNIQNYKIPIW 120

Query: 121 PPTYFIKLGLLILTLVAIYFKNYLHPTLYLIVFCFVIYFLLEILIGGAAAFVKATLGVEL 180
              + IK+ + ++ L    +K YL P L L+     I+  LEI+     A V  TLG +L
Sbjct: 121 --LFAIKVVIFVVLLQMYEYKQYLSPALLLVFNSLHIFLELEIVFMLVKALVFITLGCDL 180

Query: 181 LPYFDEPYLSVSLQDFWGKRWNLLTSRILRLAVYDPCRKLTVGIVGKKASAMMALTATFA 240
            P  +EPYL+ SLQDFWG+RWNL+   ILR AVY P R++    V    +  + + A F 
Sbjct: 181 EPQSNEPYLATSLQDFWGRRWNLMVPAILRPAVYLPARRMACRKVNSDQAMFLGVFAAFL 240

Query: 241 VSGLMHELIYFYMGRLAPTWEVTCFFLLHGVSLSAEMAMKSA--VRGRLKVPRIISTGFT 300
           VSG +HE+++FY+ R  PT EVT FFLLHGV   AE+A+K +  VR   +V   +S   T
Sbjct: 241 VSGAVHEMLFFYLTREVPTGEVTWFFLLHGVCTVAEVAVKKSTFVRRWWRVSPTVSRLLT 300

Query: 301 WWFVMTTSSWLFFP 312
             FV+ TS W FFP
Sbjct: 301 VGFVVVTSGWFFFP 310

[Close]
BLAST of Cucsa.248820 vs. TAIR10
Match: AT5G55380.1 (AT5G55380.1 MBOAT (membrane bound O-acyl transferase) family protein)

HSP 1 Score: 214.9 bits (546), Expect = 7.7e-56
Identity = 125/323 (38.70%), Postives = 182/323 (56.35%), Query Frame = 1 

Query: 1   MAAEIRIFLQTLLLVSSSLSFCFSIRNSIPNGFLRFLLILPFFSLFLYIPLQFQTIHLQG 60
           M  + R  ++  +    SLS+C+ I + +  G LR L ILP   LFL +PL    +H   
Sbjct: 1   MEEKFRNLIEVWISALISLSYCYYISSKLSKGVLRLLSILPVCILFLVLPLFLSCVHFCA 60

Query: 61  SIGFFIGWLASFKILLFAFGKGPLCSAAASSSLRRFLAIGSLPIEIPQDTNRS-----HP 120
               F+ WLA+FK+LLFAF +GPL        L RF+    LPI+I QD + +     HP
Sbjct: 61  ISVLFLSWLANFKLLLFAFDEGPLFPLPPK--LSRFICFACLPIKIRQDPSPNAIPNLHP 120

Query: 121 NPILPPTYFIKLGLLIL-TLVAIY-FKNYLHPTLYLIVFCFVIYFLLEILIGGAAAFVKA 180
            P+  P + + + +L+L  L+ +Y +++ L   + L ++C  IY  +E+++    A V  
Sbjct: 121 KPM--PKWVLAVKILVLGVLLHVYEYRDGLPRFVVLALYCLHIYLEVELVLVFVGAVVST 180

Query: 181 TLGVELLPYFDEPYLSVSLQDFWGKRWNLLTSRILRLAVYDPCRKLTVGIVGKKASAMMA 240
            LG  + P F+EPYL+ SLQDFW +RWNL+ S +LR  V+ P ++    I+    +    
Sbjct: 181 LLGCNIEPVFNEPYLATSLQDFWSRRWNLMVSAVLRSTVHIPVQRFFKRILSPDGAMFAG 240

Query: 241 LTATFAVSGLMHELIYFYMGRLAPTWEVTCFFLLHGVSLSAEMAMKSAVRGRLKVP-RII 300
           + A+F VSGLMHEL+YFYM R  PTWEVTCFF+LHG + + E+A+K      L+ P R +
Sbjct: 241 VMASFFVSGLMHELLYFYMIRKPPTWEVTCFFVLHGAATATEIAVKRT--QWLRPPHRAV 300

Query: 301 STGFTWWFVMTTSSWLFFPQFFR 316
           S      FV  T  WLF  Q  R
Sbjct: 301 SGLVVLTFVSVTGVWLFLAQVLR 317

[Close]
BLAST of Cucsa.248820 vs. NCBI nr
Match: gi|778682706|ref|XP_004149741.2| (PREDICTED: acyl-CoA--sterol O-acyltransferase 1-like [Cucumis sativus])

HSP 1 Score: 689.9 bits (1779), Expect = 2.3e-195
Identity = 345/348 (99.14%), Postives = 347/348 (99.71%), Query Frame = 1 

Query: 1   MAAEIRIFLQTLLLVSSSLSFCFSIRNSIPNGFLRFLLILPFFSLFLYIPLQFQTIHLQG 60
           MAAEIRIFLQTLLLVSSSLSFCFSIRNSIPNGFLRFLLILPFFSLFLYIPLQFQTIHLQG
Sbjct: 1   MAAEIRIFLQTLLLVSSSLSFCFSIRNSIPNGFLRFLLILPFFSLFLYIPLQFQTIHLQG 60

Query: 61  SIGFFIGWLASFKILLFAFGKGPLCSAAASSSLRRFLAIGSLPIEIPQDTNRSHPNPILP 120
           SIGFFIGWLASFK+LLFAFGKGPLCSAAASSSLRRFLAIGSLPIEIPQDTNRSHPNPILP
Sbjct: 61  SIGFFIGWLASFKLLLFAFGKGPLCSAAASSSLRRFLAIGSLPIEIPQDTNRSHPNPILP 120

Query: 121 PTYFIKLGLLILTLVAIYFKNYLHPTLYLIVFCFVIYFLLEILIGGAAAFVKATLGVELL 180
           PTYFIKL LLILTLVAIYFKNYLHPTLYLIVFCFVIYFLLEILIGGAAAFVKATLGVELL
Sbjct: 121 PTYFIKLLLLILTLVAIYFKNYLHPTLYLIVFCFVIYFLLEILIGGAAAFVKATLGVELL 180

Query: 181 PYFDEPYLSVSLQDFWGKRWNLLTSRILRLAVYDPCRKLTVGIVGKKASAMMALTATFAV 240
           PYFDEPYLSVSLQDFWGKRWNLLTSRILRLAVYDPCRKLTVGIVGKKASAM+ALTATFAV
Sbjct: 181 PYFDEPYLSVSLQDFWGKRWNLLTSRILRLAVYDPCRKLTVGIVGKKASAMVALTATFAV 240

Query: 241 SGLMHELIYFYMGRLAPTWEVTCFFLLHGVSLSAEMAMKSAVRGRLKVPRIISTGFTWWF 300
           SGLMHELIYFYMGRLAPTWEVTCFFLLHGVSLSAEMAMKSAVRGRLKVPRIISTGFTWWF
Sbjct: 241 SGLMHELIYFYMGRLAPTWEVTCFFLLHGVSLSAEMAMKSAVRGRLKVPRIISTGFTWWF 300

Query: 301 VMTTSSWLFFPQFFRLKPDVRMMEEHAALGAFVKNVTVQLITPFKFSF 349
           VMTTSSWLFFPQFFRLKPDVRMMEEHAALGAFVKNVTVQLITPFKFSF
Sbjct: 301 VMTTSSWLFFPQFFRLKPDVRMMEEHAALGAFVKNVTVQLITPFKFSF 348

[Close]
BLAST of Cucsa.248820 vs. NCBI nr
Match: gi|659123779|ref|XP_008461835.1| (PREDICTED: acyl-CoA--sterol O-acyltransferase 1-like [Cucumis melo])

HSP 1 Score: 624.8 bits (1610), Expect = 9.2e-176
Identity = 306/345 (88.70%), Postives = 323/345 (93.62%), Query Frame = 1 

Query: 2   AAEIRIFLQTLLLVSSSLSFCFSIRNSIPNGFLRFLLILPFFSLFLYIPLQFQTIHLQGS 61
           AAEIRIFLQTL LVSSSLSFCFSIRNSIPNGF +FL +LPFF LFLY+PLQFQT+HLQG 
Sbjct: 3   AAEIRIFLQTLFLVSSSLSFCFSIRNSIPNGFPKFLFLLPFFFLFLYLPLQFQTLHLQGL 62

Query: 62  IGFFIGWLASFKILLFAFGKGPLCSAAASSSLRRFLAIGSLPIEIPQDTNRSHPNPILPP 121
            GFFIGWLASFK+LLFAFGKGPL S AASSSLRRFLAIGSLPIEIPQDTN S PNPILP 
Sbjct: 63  FGFFIGWLASFKLLLFAFGKGPLSSPAASSSLRRFLAIGSLPIEIPQDTNPSDPNPILPH 122

Query: 122 TYFIKLGLLILTLVAIYFKNYLHPTLYLIVFCFVIYFLLEILIGGAAAFVKATLGVELLP 181
           TYFIKLGLLILT VA+YF+ YLHP LYLI FCF+IYFLLEILIGGAAAFVKATLGVELLP
Sbjct: 123 TYFIKLGLLILTFVAVYFRTYLHPNLYLIAFCFLIYFLLEILIGGAAAFVKATLGVELLP 182

Query: 182 YFDEPYLSVSLQDFWGKRWNLLTSRILRLAVYDPCRKLTVGIVGKKASAMMALTATFAVS 241
           YFDEPYLS SLQDFWGKRWNL+ SRILRLAVYDPCRKLT+GIVGKK SA++A+TATFAVS
Sbjct: 183 YFDEPYLSASLQDFWGKRWNLMASRILRLAVYDPCRKLTIGIVGKKPSAIVAMTATFAVS 242

Query: 242 GLMHELIYFYMGRLAPTWEVTCFFLLHGVSLSAEMAMKSAVRGRLKVPRIISTGFTWWFV 301
           GLMHELIYFYMGR+APTWEVTCFFLLHGVSLSAEMAMKSAV GR +VPRI+STGFTWWFV
Sbjct: 243 GLMHELIYFYMGRVAPTWEVTCFFLLHGVSLSAEMAMKSAVGGRFRVPRIVSTGFTWWFV 302

Query: 302 MTTSSWLFFPQFFRLKPDVRMMEEHAALGAFVKNVTVQLITPFKF 347
           MTTSSWLFFPQFFRLKP+VRM EEHAAL AFVKNVTVQLITPFKF
Sbjct: 303 MTTSSWLFFPQFFRLKPEVRMFEEHAALWAFVKNVTVQLITPFKF 347

[Close]
BLAST of Cucsa.248820 vs. NCBI nr
Match: gi|659123781|ref|XP_008461836.1| (PREDICTED: acyl-CoA--sterol O-acyltransferase 1-like [Cucumis melo])

HSP 1 Score: 476.9 bits (1226), Expect = 3.1e-131
Identity = 236/348 (67.82%), Postives = 275/348 (79.02%), Query Frame = 1 

Query: 1   MAAEIRIFLQTLLLVSSSLSFCFSIRNSIPNGFLRFLLILPFFSLFLYIPLQFQTIHLQG 60
           MAAEI   LQTL LVSSSLS CFSIRNSIPNGFL+FLL+LPFF LFLYIPLQF TIH QG
Sbjct: 1   MAAEIHSLLQTLFLVSSSLSICFSIRNSIPNGFLKFLLVLPFFFLFLYIPLQFHTIHFQG 60

Query: 61  SIGFFIGWLASFKILLFAFGKGPLCSAAASSSLRRFLAIGSLPIEIPQDTNRSHPNPILP 120
            IGFFIGWL SFK+LLFAFG+GPLCSAA SSSL RFLA+GSLPIEIP   +  H + +LP
Sbjct: 61  PIGFFIGWLGSFKLLLFAFGRGPLCSAATSSSLPRFLAVGSLPIEIPDHKSPPH-HSLLP 120

Query: 121 PTYFIKLGLLILTLVAIYFKNYLHPTLYLIVFCFVIYFLLEILIGGAAAFVKATLGVELL 180
           P+   KLG LILT++AI FKN+ H    L+ +C +IYF LE LIG  AA  K  LGVELL
Sbjct: 121 PS--AKLGFLILTILAINFKNHFHQNAVLLFYCLLIYFFLEFLIGTTAALAKKVLGVELL 180

Query: 181 PYFDEPYLSVSLQDFWGKRWNLLTSRILRLAVYDPCRKLTVGIVGKKASAMMALTATFAV 240
           PYF+EPYLS SLQDFWG+RWNL+TSRILRL VYDPCR LTVGI+G++ ++M+A+ ATF V
Sbjct: 181 PYFNEPYLSDSLQDFWGRRWNLMTSRILRLTVYDPCRNLTVGIIGRRRASMVAVMATFGV 240

Query: 241 SGLMHELIYFYMGRLAPTWEVTCFFLLHGVSLSAEMAMKSAVRGRLKVPRIISTGFTWWF 300
           SGLMHELIYFYMGR+APTWEV+CFF++HGV + AE A+K    GR + PR +  G T  F
Sbjct: 241 SGLMHELIYFYMGRMAPTWEVSCFFVVHGVCVVAERAVKLGAGGRYRAPRAVRIGLTIGF 300

Query: 301 VMTTSSWLFFPQFFRLKPDVRMMEEHAALGAFVKNVTVQLITPFKFSF 349
           VM T SWLFFPQ  R K DVRM+EE+AA+GAF+K++TV  I    F F
Sbjct: 301 VMGTGSWLFFPQCIRAKMDVRMLEEYAAMGAFLKDITVLFIDSISFLF 345

[Close]
BLAST of Cucsa.248820 vs. NCBI nr
Match: gi|449464052|ref|XP_004149743.1| (PREDICTED: acyl-CoA--sterol O-acyltransferase 1-like [Cucumis sativus])

HSP 1 Score: 469.2 bits (1206), Expect = 6.5e-129
Identity = 231/345 (66.96%), Postives = 272/345 (78.84%), Query Frame = 1 

Query: 1   MAAEIRIFLQTLLLVSSSLSFCFSIRNSIPNGFLRFLLILPFFSLFLYIPLQFQTIHLQG 60
           MA+EI   LQTL LVSSSLS CFSIRNSIPNGF +FLLILPFF LFLYIPLQF TIH QG
Sbjct: 1   MASEIHSLLQTLFLVSSSLSLCFSIRNSIPNGFFKFLLILPFFFLFLYIPLQFHTIHFQG 60

Query: 61  SIGFFIGWLASFKILLFAFGKGPLCSAAASSSLRRFLAIGSLPIEIPQDTNRSHPN-PIL 120
            IGFFIGWL SFK+LLFAFGKGPLCSAA SSSL RFLA+GSLPIEIP   ++S PN  ++
Sbjct: 61  PIGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLPRFLAVGSLPIEIPD--HKSPPNHSLI 120

Query: 121 PPTYFIKLGLLILTLVAIYFKNYLHPTLYLIVFCFVIYFLLEILIGGAAAFVKATLGVEL 180
           PP+   KL  LILT++AI FKN+LHP    + +C +IYF LE LIG  A   K  LGVEL
Sbjct: 121 PPS--AKLIFLILTILAINFKNHLHPNAIPLFYCLLIYFFLEFLIGTTATLAKTVLGVEL 180

Query: 181 LPYFDEPYLSVSLQDFWGKRWNLLTSRILRLAVYDPCRKLTVGIVGKKASAMMALTATFA 240
           LPYF+EPY S SLQDFWG+RWNL+TSRILRL +YDPCR LT+G++G++ ++M+A+ ATF 
Sbjct: 181 LPYFNEPYFSDSLQDFWGRRWNLMTSRILRLTIYDPCRNLTIGVIGRRPASMVAVIATFV 240

Query: 241 VSGLMHELIYFYMGRLAPTWEVTCFFLLHGVSLSAEMAMKSAVRGRLKVPRIISTGFTWW 300
           VSGLMHELIYFYMGR+APTWEVTCFF++HGV + AEMA++    GR + PR+     T  
Sbjct: 241 VSGLMHELIYFYMGRMAPTWEVTCFFVVHGVCVVAEMAVRLGAGGRFRAPRVARICLTIM 300

Query: 301 FVMTTSSWLFFPQFFRLKPDVRMMEEHAALGAFVKNVTVQLITPF 345
           FVM T SWLFFPQ  R K DVRM+EE+AA+GAF K +T+  I PF
Sbjct: 301 FVMGTGSWLFFPQCIRAKMDVRMLEEYAAIGAFFKTITLHFIPPF 341

[Close]
BLAST of Cucsa.248820 vs. NCBI nr
Match: gi|658060478|ref|XP_008366078.1| (PREDICTED: acyl-CoA--sterol O-acyltransferase 1-like [Malus domestica])

HSP 1 Score: 331.3 bits (848), Expect = 2.1e-87
Identity = 175/351 (49.86%), Postives = 233/351 (66.38%), Query Frame = 1 

Query: 1   MAAEIRIFLQTLLLVSSSLSFCFSIRNSIPNGFLRFLLILPFFSLFLYIPLQFQTIHLQG 60
           M  EI  F++  L V  SL +C++I   +P G  R LLI+P  SLFLY+PL   ++HL G
Sbjct: 1   MEGEIGNFVKVWLSVYLSLCYCYAIAKFVPKGVPRLLLIIPIVSLFLYLPLHLSSLHLGG 60

Query: 61  SIGFFIGWLASFKILLFAFGKGPLCSAAASSSLRRFLAIGSLPIEIPQDT-----NRSHP 120
           +  FFI WLA+FK+LLFAFGKGPLCS   S SL RF+A+  LPI+I Q+      N+ +P
Sbjct: 61  TTAFFISWLANFKLLLFAFGKGPLCSDP-SISLLRFVAVACLPIKIQQNQYSKTQNKENP 120

Query: 121 NPIL-------PPTYFIKLGLLILTLVAIY-FKNYLHPTLYLIVFCFVIYFLLEILIGGA 180
            P +       P  Y +K GLL   LV +Y +K ++HP L  I++CF IYF LEI++  A
Sbjct: 121 APKITKNGTKSPLNYAVK-GLLFALLVKLYDYKEHIHPKLLWIMYCFHIYFALEIILVIA 180

Query: 181 AAFVKATLGVELLPYFDEPYLSVSLQDFWGKRWNLLTSRILRLAVYDPCRKLTVGIVGKK 240
           A   +A LG+EL P FDEPYLS SLQDFWG+RWN++ + ILR  VY+P   L   +VG++
Sbjct: 181 ATLARALLGLELEPQFDEPYLSSSLQDFWGRRWNIMVTSILRPTVYEPILALATRVVGRR 240

Query: 241 ASAMMALTATFAVSGLMHELIYFYMGRLAPTWEVTCFFLLHGVSLSAEMAMKSAV--RGR 300
            + +  +  TF VS +MHELI++Y+GR  PTWE+T FFLLHG  L AE+ +K AV  R +
Sbjct: 241 WATLAGVMGTFLVSAIMHELIFYYLGRTRPTWEITWFFLLHGACLVAEIGVKKAVGKRKK 300

Query: 301 LKVPRIISTGFTWWFVMTTSSWLFFPQFFRLKPDVRMMEEHAALGAFVKNV 337
           L++PR +S   T  FVM T  WLFFP   R K D+R +EE+AA+GAFVKN+
Sbjct: 301 LQLPRSVSGPLTVAFVMATVFWLFFPPLLRCKADMRALEEYAAVGAFVKNI 349

The following BLAST results are available for this feature:
BLAST of Cucsa.248820 vs. Swiss-Prot
Match NameE-valueIdentityDescription
ASAT1_ARATH7.5e-6941.72Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana GN=ASAT1 PE=1 SV=1[more]
WAXS1_SIMCH1.1e-5937.18Long-chain-alcohol O-fatty-acyltransferase OS=Simmondsia chinensis PE=1 SV=1[more]
WAXS2_ARATH3.5e-5839.76Probable long-chain-alcohol O-fatty-acyltransferase 2 OS=Arabidopsis thaliana GN... [more]
WAXS4_ARATH1.0e-5737.98Probable long-chain-alcohol O-fatty-acyltransferase 4 OS=Arabidopsis thaliana GN... [more]
WAXS3_ARATH3.0e-5740.13Probable long-chain-alcohol O-fatty-acyltransferase 3 OS=Arabidopsis thaliana GN... [more]
BLAST of Cucsa.248820 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LES4_CUCSA1.6e-19599.14Uncharacterized protein OS=Cucumis sativus GN=Csa_3G684670 PE=4 SV=1[more]
A0A0A0L9R8_CUCSA4.5e-12966.96Uncharacterized protein OS=Cucumis sativus GN=Csa_3G683670 PE=4 SV=1[more]
A0A061GG81_THECC2.3e-8545.33Acyl-CoA sterol acyl transferase 1, putative OS=Theobroma cacao GN=TCM_030359 PE... [more]
B9HNV0_POPTR6.8e-8547.93Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0009s05990g PE=4 SV=1[more]
M5X7K4_PRUPE3.4e-8447.11Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa018212mg PE=4 SV=1[more]
BLAST of Cucsa.248820 vs. TAIR10
Match NameE-valueIdentityDescription
AT3G51970.14.2e-7041.72 acyl-CoA sterol acyl transferase 1[more]
AT5G55370.12.0e-5939.76 MBOAT (membrane bound O-acyl transferase) family protein[more]
AT5G55350.15.7e-5937.98 MBOAT (membrane bound O-acyl transferase) family protein[more]
AT5G55360.11.7e-5840.13 MBOAT (membrane bound O-acyl transferase) family protein[more]
AT5G55380.17.7e-5638.70 MBOAT (membrane bound O-acyl transferase) family protein[more]
BLAST of Cucsa.248820 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|778682706|ref|XP_004149741.2|2.3e-19599.14PREDICTED: acyl-CoA--sterol O-acyltransferase 1-like [Cucumis sativus][more]
gi|659123779|ref|XP_008461835.1|9.2e-17688.70PREDICTED: acyl-CoA--sterol O-acyltransferase 1-like [Cucumis melo][more]
gi|659123781|ref|XP_008461836.1|3.1e-13167.82PREDICTED: acyl-CoA--sterol O-acyltransferase 1-like [Cucumis melo][more]
gi|449464052|ref|XP_004149743.1|6.5e-12966.96PREDICTED: acyl-CoA--sterol O-acyltransferase 1-like [Cucumis sativus][more]
gi|658060478|ref|XP_008366078.1|2.1e-8749.86PREDICTED: acyl-CoA--sterol O-acyltransferase 1-like [Malus domestica][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008152 metabolic process
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0016740 transferase activity
molecular_function GO:0003674 molecular_function
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.248820.1Cucsa.248820.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR31595FAMILY NOT NAMEDcoord: 1..344
score: 4.2E
NoneNo IPR availablePANTHERPTHR31595:SF13ACYL-COA--STEROL O-ACYLTRANSFERASE 1coord: 1..344
score: 4.2E