Cucsa.248400 (gene) Cucumber (Gy14) v1

NameCucsa.248400
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionRetrovirus-related Pol polyprotein from transposon TNT 1-94
Locationscaffold02207 : 227514 .. 230470 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TGATAATGAAAGAAAATTCCAAAATCATAACCTGAGTGAATTTCTAGCTTCGAAAGGGATTGTTCATCAAAACACTTGCGCCTACACTCCTCAACAAAATGGAGTGGCCGAGCGAAAGAACTATCACCTTCTGGAAGCAGCCCGTTCCCGTATGCTTTCCACTTCCCTCCCTTCATACTTGTGGGGAGATACTATTATTACAACAACTCATTTAATCAATAGAATTCCTTCTCGTATTCTCCACCTTCAGACTCCCTTAGATTTTCTTAAGGAGTCCTACCCATCTACTCGTCTCATTTATGAGGTTCCTCTTCATGTGTTTGGATGTACAACTTATGTTCATAATTTTGACCCTAATCAGACCAAATTTACCCATCAAGCTCAGACATGTGTGTTTGTTGGGTATCCCCTTCACCAACGTGGTTATAAATGTTTTCACCCGCCATCACTACAAGAATTAGGGTCTTCTCCAATGCAGCAACACGTCGTCGAAAGCCTTAAAAATGTAGTGTAAAGTTTTTGCAACGTTGTATGCGACGTCGGCATGCACGTAATGGAAAGCCCGTCGGGGATACTTTCCGCAACGTACCTTGCGCCAACGATGGCGAAACTTTCCTTAGTGCATAGATCAACACGTCAGATAAGGCTTCGGGGCGTGGCTTCTTCGAGGACTACCTCGATGCAGATGTTGCAAAAACCTATTGCGACGAAGCGTCACAATAGGTTTTCACGACGTTTGCGTCGGGGAAGGTTTTTTATTATTTTTTTTTATATTTCATATTTTTCTTGTATTATTATGATATTTTTATTGTTTTCTATTATTTATTTTACTAAGGCCTGATGTAATAAAAATTGTAATAAATACTTTATCACTATGAATGTTACTTTCTCTGAGGCCCAACATTACTTTCTCGTTAGCCATCCTCAAGGGGACAGTGTGAGTGAAGAGTCTAACAACACCTTTGAATTTATCAAACCTACTCCTAGCGTCGTGTCAGACATCGATCCTCATCTCATAGTCCTACCCACAAACCAAGTTCACTAGAAAACGTATTACAGGAGAAGTCTAAAAAAGGAAGTCGCTTTCCCAACTAGTCAGTCATCGACTGCAGTCCAAGACTCTGAACCTCCTCGAGATCAAGGTATGGAAAATCCTACTAAATCGTGTACTAATAATATAATAAGTGTAAATGACTTGTCTAATGTTGTTATTCTTGAAAATGTGGAAGAAAAGAATAGTAGTGATGAGACTGAGGTCGGGACAAAAACTAGTAATAATGAAGTTGAACAGGGTTATGCAGAAAAACTTGATGAGTATGATTCTTCTCTTGACATTCTCATTGCTCTGTGAAAAGGTACTAGGTCTTATACTAAACATCCCATTTGCAACTTTGTTTCCTATGATAATCTCTCTCCACAGTTCAGAGTTTTTACAGCAACCCTTGACTCTACCATAATACCAAAAAATATCTACACTGCTTTAGAGTGTCTTGAAAAGAATAGTATTTGGGACGTTTGTACTCTACCCAAGGGGCACAAAACTATGGGATGCAAATGAGTGTTCTCTCTCAAATACAGAGTAGATGGTACTCTTGACAGACACAAGGCAAGGTTAGTTGCAAAGGGATTTACTCAAACCTATGCTATTGACTATTCAGAGACTTTTTCTCTAGATGCTAAGTTGAATACTATTAGAGTTATGCTATATGTTGTTGTGAACAAAGAGTGGCCTTTATATTAGCTAGATGTTAATAATGTTTTTCTGAATGGAGACCTTGTGGAGTAAGTCTACATGAGCTCCCCACTTGGATTTGAAGCTCAGTTTGGTCAGCAGGTGTGTAAACTCTAGAAATCCCTATATGGCCTGAAACAGTCTCCTAGAACATGGTTTGACAGATTCACTACCTTTGTCAAGTCCTAAGGGTATAGTCAGGGGCACCCTGATCATACTTTATTTACAAAGGTTTCCAAGATAGGAAAGATTGTTGTGCTAATAGTATATATGGATGACATTTTTTTGAATGGAGATGATCAGGAAGAAATCAGTTAACTAAAGTAGAGAATGGATGATAAATTTGAAATCAAGGATTTGAGAAATCTAAAATATTTCCTTGGAATGAAGGTGGCTTGATCTAAAGAAGGTATCTTCGTATCTCAAAGAAAATACACTCTTGATTTGCTAGCTGAGGTATGTTGGGATGTCATCCCGCTGACACTCCTATTCAATTCAATTATAAACTAGGAAACTCTTATGATCAAGTTCCATTTGATAAAGAACAATAGTAGTGCCTCGTGGGTAAATTAATTTACTTATTCCATACTCGTCGTGATATTTTCTTTGTTGTGAGTGTTGTCAGCCAGTTTATGTAGGCTCCATATAAGGAATACATGAAAGCTGTCAACAAAATTTTGAGATACTTGAAATCAACACTTGACTGATGTTTAGAAAAACAGATAGAAAGACCACTGAGGCAAATACTGACTCGGCTTGGAGAGGATCTATTATTGACAGAAAGTCTACTTTTGGTTATTGTACCTTTGTTTGGGGCAATCTTGTAACTTGGAGGATTAAGAAGCAAAGTGTTGTGGCTAGGAGCAGCGCTGAGGCCGAATACAAAGCTATGAACTTGGGAATATGTAAGGAAATTTGGCTTCAGAAAGTCCTGTCAGATCTTCATCAGGAATGTGAGACACCATTGAAGCTTTTTTGTGATAATAAAGCCGCTATTAGTATTGCTAACAACCATGTTCAACATGATAGAACTAAACATGTTGAGATTGATCGATATTTCATCAAAGAAAGACTTGATTGTGGGAGCATATGCATTCCGTACATCCATTCGAGTCAACAGGTTGCTGATGTTCTTACCAATGGGCTTCTCATACCAAACTTCGACTTTTATATTAGTAAGTTGCGCCTAATTGATATTTACGTTCAAATTGAGGGGGAATGTTAG

mRNA sequence

tgataatgaaagaaaattccaaaatcataacctgagtgaatttctagcttcgaaagggattgttcatcaaaacacttgcgcctacactcctcaacaaaatggagtggccgagcgaaagaactatcaccttctggaagcagcccgttcccgtatgctttccacttccctcccttcatacttgtggggagatactattattacaacaactcatttaatcaatagaattccttctcgtattctccaccttcagactcccttagattttcttaaggagtcctacccatctactcgtctcatttatgaggttcctcttcatgtgtttggatgtacaacttatgttcataattttgaccctaatcagaccaaatttacccatcaagctcagacatgtgtgtttgttgggtatccccttcaccaacgtggttataaatgttttcacccgccatcactacaagaattagggagaagtctaaaaaaggaagtcgctttcccaactagtcagtcatcgactgcagtccaagactctgaacctcctcgagatcaaggtatggaaaatcctactaaatcgtgtactaataatataataagtgtaaatgacttgtctaatgttgttattcttgaaaatgtggaagaaaagaatagtagtgatgagactgaggtcgggacaaaaactagtaataatgaagttgaacagggtactaggtcttatactaaacatcccatttgcaactttgtttcctatgataatctctctccacagttcagagtttttacagcaacccttgactctaccataataccaaaaaatatctacactgctttagaagtagatggtactcttgacagacacaaggcaaggttagttgcaaagggatttactcaaacctatgctattgactattcagagactttttctctagatgctaagttgaatactattagagttatgctatatgttgttgtgaacaaagaaaaaacagatagaaagaccactgaggcaaatactgactcggcttggagaggatctattattgacagaaagtctacttttggttattgtacctttgtttggggcaatcttgtaacttggaggattaagaagcaaagtgttgtggctaggagcagcgctgaggccgaatacaaagctatgaacttgggaatatgtaaggaaatttggcttcagaaagtcctgtcagatcttcatcaggaatgtgagacaccattgaagcttttttgtgataataaagccgctattagtattgctaacaaccatgttcaacatgatagaactaaacatgttgagattgatcgatatttcatcaaagaaagacttgattgtgggagcatatgcattccgtacatccattcgagtcaacaggttgctgatgttcttaccaatgggcttctcataccaaacttcgacttttatattagtaagttgcgcctaattgatatttacgttcaaattgagggggaatgttag

Coding sequence (CDS)

TGATAATGAAAGAAAATTCCAAAATCATAACCTGAGTGAATTTCTAGCTTCGAAAGGGATTGTTCATCAAAACACTTGCGCCTACACTCCTCAACAAAATGGAGTGGCCGAGCGAAAGAACTATCACCTTCTGGAAGCAGCCCGTTCCCGTATGCTTTCCACTTCCCTCCCTTCATACTTGTGGGGAGATACTATTATTACAACAACTCATTTAATCAATAGAATTCCTTCTCGTATTCTCCACCTTCAGACTCCCTTAGATTTTCTTAAGGAGTCCTACCCATCTACTCGTCTCATTTATGAGGTTCCTCTTCATGTGTTTGGATGTACAACTTATGTTCATAATTTTGACCCTAATCAGACCAAATTTACCCATCAAGCTCAGACATGTGTGTTTGTTGGGTATCCCCTTCACCAACGTGGTTATAAATGTTTTCACCCGCCATCACTACAAGAATTAGGGAGAAGTCTAAAAAAGGAAGTCGCTTTCCCAACTAGTCAGTCATCGACTGCAGTCCAAGACTCTGAACCTCCTCGAGATCAAGGTATGGAAAATCCTACTAAATCGTGTACTAATAATATAATAAGTGTAAATGACTTGTCTAATGTTGTTATTCTTGAAAATGTGGAAGAAAAGAATAGTAGTGATGAGACTGAGGTCGGGACAAAAACTAGTAATAATGAAGTTGAACAGGGTACTAGGTCTTATACTAAACATCCCATTTGCAACTTTGTTTCCTATGATAATCTCTCTCCACAGTTCAGAGTTTTTACAGCAACCCTTGACTCTACCATAATACCAAAAAATATCTACACTGCTTTAGAAGTAGATGGTACTCTTGACAGACACAAGGCAAGGTTAGTTGCAAAGGGATTTACTCAAACCTATGCTATTGACTATTCAGAGACTTTTTCTCTAGATGCTAAGTTGAATACTATTAGAGTTATGCTATATGTTGTTGTGAACAAAGAAAAAACAGATAGAAAGACCACTGAGGCAAATACTGACTCGGCTTGGAGAGGATCTATTATTGACAGAAAGTCTACTTTTGGTTATTGTACCTTTGTTTGGGGCAATCTTGTAACTTGGAGGATTAAGAAGCAAAGTGTTGTGGCTAGGAGCAGCGCTGAGGCCGAATACAAAGCTATGAACTTGGGAATATGTAAGGAAATTTGGCTTCAGAAAGTCCTGTCAGATCTTCATCAGGAATGTGAGACACCATTGAAGCTTTTTTGTGATAATAAAGCCGCTATTAGTATTGCTAACAACCATGTTCAACATGATAGAACTAAACATGTTGAGATTGATCGATATTTCATCAAAGAAAGACTTGATTGTGGGAGCATATGCATTCCGTACATCCATTCGAGTCAACAGGTTGCTGATGTTCTTACCAATGGGCTTCTCATACCAAACTTCGACTTTTATATTAGTAAGTTGCGCCTAATTGATATTTACGTTCAAATTGAGGGGGAATGTTAG

Protein sequence

DNERKFQNHNLSEFLASKGIVHQNTCAYTPQQNGVAERKNYHLLEAARSRMLSTSLPSYLWGDTIITTTHLINRIPSRILHLQTPLDFLKESYPSTRLIYEVPLHVFGCTTYVHNFDPNQTKFTHQAQTCVFVGYPLHQRGYKCFHPPSLQELGRSLKKEVAFPTSQSSTAVQDSEPPRDQGMENPTKSCTNNIISVNDLSNVVILENVEEKNSSDETEVGTKTSNNEVEQGTRSYTKHPICNFVSYDNLSPQFRVFTATLDSTIIPKNIYTALEVDGTLDRHKARLVAKGFTQTYAIDYSETFSLDAKLNTIRVMLYVVVNKEKTDRKTTEANTDSAWRGSIIDRKSTFGYCTFVWGNLVTWRIKKQSVVARSSAEAEYKAMNLGICKEIWLQKVLSDLHQECETPLKLFCDNKAAISIANNHVQHDRTKHVEIDRYFIKERLDCGSICIPYIHSSQQVADVLTNGLLIPNFDFYISKLRLIDIYVQIEGEC*
BLAST of Cucsa.248400 vs. Swiss-Prot
Match: COPIA_DROME (Copia protein OS=Drosophila melanogaster GN=GIP PE=1 SV=3)

HSP 1 Score: 115.2 bits (287), Expect = 2.1e-24
Identity = 61/149 (40.94%), Postives = 91/149 (61.07%), Query Frame = 1

Query: 336  DSAWRGSIIDRKSTFGYCTFVWG-NLVTWRIKKQSVVARSSAEAEYKAMNLGICKEIWLQ 395
            DS W GS IDRKST GY   ++  NL+ W  K+Q+ VA SS EAEY A+   + + +WL+
Sbjct: 1253 DSDWAGSEIDRKSTTGYLFKMFDFNLICWNTKRQNSVAASSTEAEYMALFEAVREALWLK 1312

Query: 396  KVLSDLHQECETPLKLFCDNKAAISIANNHVQHDRTKHVEIDRYFIKERLDCGSICIPYI 455
             +L+ ++ + E P+K++ DN+  ISIANN   H R KH++I  +F +E++    IC+ YI
Sbjct: 1313 FLLTSINIKLENPIKIYEDNQGCISIANNPSCHKRAKHIDIKYHFAREQVQNNVICLEYI 1372

Query: 456  HSSQQVADVLTNGLLIPNFDFYISKLRLI 484
             +  Q+AD+ T  L    F     KL L+
Sbjct: 1373 PTENQLADIFTKPLPAARFVELRDKLGLL 1401


HSP 2 Score: 88.6 bits (218), Expect = 2.1e-16
Identity = 52/137 (37.96%), Postives = 73/137 (53.28%), Query Frame = 1

Query: 1   DNERKFQNHNLSEFLASKGIVHQNTCAYTPQQNGVAERKNYHLLEAARSRMLSTSLPSYL 60
           DN R++ ++ + +F   KGI +  T  +TPQ NGV+ER    + E AR+ +    L    
Sbjct: 550 DNGREYLSNEMRQFCVKKGISYHLTVPHTPQLNGVSERMIRTITEKARTMVSGAKLDKSF 609

Query: 61  WGDTIITTTHLINRIPSRIL--HLQTPLDFLKESYPSTRLIYEVPLHVFGCTTYVHNFDP 120
           WG+ ++T T+LINRIPSR L    +TP +      P     Y   L VFG T YVH    
Sbjct: 610 WGEAVLTATYLINRIPSRALVDSSKTPYEMWHNKKP-----YLKHLRVFGATVYVH-IKN 669

Query: 121 NQTKFTHQAQTCVFVGY 136
            Q KF  ++   +FVGY
Sbjct: 670 KQGKFDDKSFKSIFVGY 680


HSP 3 Score: 48.9 bits (115), Expect = 1.8e-04
Identity = 22/40 (55.00%), Postives = 32/40 (80.00%), Query Frame = 1

Query: 282 RHKARLVAKGFTQTYAIDYSETFSLDAKLNTIRVMLYVVV 322
           R+KARLVA+GFTQ Y IDY ETF+  A++++ R +L +V+
Sbjct: 953 RYKARLVARGFTQKYQIDYEETFAPVARISSFRFILSLVI 992

BLAST of Cucsa.248400 vs. Swiss-Prot
Match: POLX_TOBAC (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 7.0e-20
Identity = 71/241 (29.46%), Postives = 110/241 (45.64%), Query Frame = 1

Query: 1   DNERKFQNHNLSEFLASKGIVHQNTCAYTPQQNGVAERKNYHLLEAARSRMLSTSLPSYL 60
           DN  ++ +    E+ +S GI H+ T   TPQ NGVAER N  ++E  RS +    LP   
Sbjct: 550 DNGGEYTSREFEEYCSSHGIRHEKTVPGTPQHNGVAERMNRTIVEKVRSMLRMAKLPKSF 609

Query: 61  WGDTIITTTHLINRIPSRILHLQTPLDFLKESYPSTRLIYEVPLHVFGCTTYVHNFDPNQ 120
           WG+ + T  +LINR PS  L  + P     E   + + +    L VFGC  + H     +
Sbjct: 610 WGEAVQTACYLINRSPSVPLAFEIP-----ERVWTNKEVSYSHLKVFGCRAFAHVPKEQR 669

Query: 121 TKFTHQAQTCVFVGYPLHQRGYKCFHPPSLQELGRSLKKEVAFPTSQSSTAVQDSEPPRD 180
           TK   ++  C+F+GY   + GY+ + P   + +     ++V F  S+  TA   SE  ++
Sbjct: 670 TKLDDKSIPCIFIGYGDEEFGYRLWDPVKKKVI---RSRDVVFRESEVRTAADMSEKVKN 729

Query: 181 QGMEN--PTKSCTNNIISVNDLSNVVILENVEEKNSSDETEVGTKTSNNEVEQGTRSYTK 240
             + N     S +NN  S    ++ V  E  E+     E          EVE  T+   +
Sbjct: 730 GIIPNFVTIPSTSNNPTSAESTTDEV-SEQGEQPGEVIEQGEQLDEGVEEVEHPTQGEEQ 781


HSP 2 Score: 84.7 bits (208), Expect = 3.0e-15
Identity = 50/147 (34.01%), Postives = 86/147 (58.50%), Query Frame = 1

Query: 335  TDSAWRGSIIDRKSTFGYCTFVWGNLVTWRIKKQSVVARSSAEAEYKAMNLGICKEIWLQ 394
            TD+   G I +RKS+ GY     G  ++W+ K Q  VA S+ EAEY A      + IWL+
Sbjct: 1179 TDADMAGDIDNRKSSTGYLFTFSGGAISWQSKLQKCVALSTTEAEYIAATETGKEMIWLK 1238

Query: 395  KVLSDL--HQECETPLKLFCDNKAAISIANNHVQHDRTKHVEIDRYFIKERLDCGSICIP 454
            + L +L  HQ+      ++CD+++AI ++ N + H RTKH+++  ++I+E +D  S+ + 
Sbjct: 1239 RFLQELGLHQK---EYVVYCDSQSAIDLSKNSMYHARTKHIDVRYHWIREMVDDESLKVL 1298

Query: 455  YIHSSQQVADVLTNGLLIPNFDFYISK 480
             I +++  AD+LT   ++P   F + K
Sbjct: 1299 KISTNENPADMLTK--VVPRNKFELCK 1320


HSP 3 Score: 41.2 bits (95), Expect = 3.8e-02
Identity = 26/64 (40.62%), Postives = 35/64 (54.69%), Query Frame = 1

Query: 277 DGTLDRHKARLVAKGFTQTYAIDYSETFSLDAKLNTIRVMLYVV----VNKEKTDRKTTE 336
           D  L R+KARLV KGF Q   ID+ E FS   K+ +IR +L +     +  E+ D KT  
Sbjct: 868 DCKLVRYKARLVVKGFEQKKGIDFDEIFSPVVKMTSIRTILSLAASLDLEVEQLDVKTAF 927

BLAST of Cucsa.248400 vs. Swiss-Prot
Match: M810_ARATH (Uncharacterized mitochondrial protein AtMg00810 OS=Arabidopsis thaliana GN=AtMg00810 PE=4 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 3.6e-08
Identity = 27/68 (39.71%), Postives = 40/68 (58.82%), Query Frame = 1

Query: 325 KTDRKTTEANTDSAWRGSIIDRKSTFGYCTFVWGNLVTWRIKKQSVVARSSAEAEYKAMN 384
           K  +   +A  DS W G    R+ST G+CTF+  N+++W  K+Q  V+RSS E EY+A+ 
Sbjct: 158 KNSKLNVQAFCDSDWAGCTSTRRSTTGFCTFLGCNIISWSAKRQPTVSRSSTETEYRALA 217

Query: 385 LGICKEIW 393
           L   +  W
Sbjct: 218 LTAAELTW 225

BLAST of Cucsa.248400 vs. TrEMBL
Match: Q7XM85_ORYSJ (OSJNBb0060E08.14 protein OS=Oryza sativa subsp. japonica GN=OSJNBb0060E08.14 PE=4 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 3.3e-61
Identity = 159/477 (33.33%), Postives = 248/477 (51.99%), Query Frame = 1

Query: 20  IVHQNTCAYTPQQNGVAERKNYHLLEAARSRMLSTSLPSYLWGDTIITTTHLINRIPSRI 79
           +VH +        NGVAE KN H+LE  RS M + ++P +LW + +++ T+LINR+PSRI
Sbjct: 88  LVHSDVWTSPIASNGVAESKNRHILEVTRSLMYTMNVPKFLWSEAVMSATYLINRMPSRI 147

Query: 80  LHLQTPLDFLKESYPSTRLIYEVPLHVFGCTTYVHNFDPNQTKFTHQAQTCVFVGYPLHQ 139
           L ++TP + +   +     I  VP  VFGCT +V +  P+  K   +A  C+F+GY   Q
Sbjct: 148 LGMKTPYEMV---FGKNEFI--VPPKVFGCTCFVRDHRPSVGKLDPRAVKCIFIGYSSGQ 207

Query: 140 RGYKCFHPPSLQ---ELGRSLKKEVAF---PTSQSSTAVQDSEPPRDQ-GMENPTKSCTN 199
           +GYKC+ P   +    +  + ++ V F    T  SS  V    P  D+ G E    S  +
Sbjct: 208 KGYKCWSPSERRTFVSMDVTFRESVPFYGERTDLSSLFVDLDNPIIDEDGQEGENGSSGD 267

Query: 200 NIISVNDLSNVVILENVEEKNSSDETEVGTKTSNNEV--EQGTRSYT--KHPICNFVSYD 259
                +D  + +    +      +E E G + +N  +   +G RS    ++P  N   Y 
Sbjct: 268 K---PSDQCDTI---QISSDTEGEEFETGGEETNLPIAIRKGVRSNAVKQNPDGNVERYK 327

Query: 260 N---LSPQFRVFTATLDSTIIPKNIYTALEVD-----GTLDRHKARLVAKGFTQ------ 319
                    + +    D T  P    + L+V      G L       +  GF        
Sbjct: 328 ARLVAKGYSQTYGIDYDETFAPVPKMSTLDVKNAFLHGDLQEEVYMEIPPGFATSQTEDA 387

Query: 320 --TYAIDYSETFSLDAKLNTI----RVMLYVVVNK------EKTDRKTTEANTDSAWRGS 379
             TYA+     +  D +   +    R++ Y+  +       +K      E   D+ W   
Sbjct: 388 DITYAVSVVSRYMHDPRSGHMDVVYRILRYLKASPGKGIWFKKNGHLDVEGYCDADWGSC 447

Query: 380 IIDRKSTFGYCTFVWGNLVTWRIKKQSVVARSSAEAEYKAMNLGICKEIWLQKVLSDLHQ 439
           + D +ST GYC F+ GNLV+WR KKQSVV+RS+AEAEY++M++ + + +WL+ +L++L  
Sbjct: 448 LDDMRSTSGYCVFIGGNLVSWRSKKQSVVSRSTAEAEYRSMSMSLSELLWLKNLLAELKL 507

Query: 440 ECETPLKLFCDNKAAISIANNHVQHDRTKHVEIDRYFIKERLDCGSICIPYIHSSQQ 460
              T +KL+CDNK+AI+IANN VQHDRTKHVEIDR+FIKER+D G++ + +++S +Q
Sbjct: 508 STSTSMKLWCDNKSAINIANNPVQHDRTKHVEIDRFFIKERMDEGTLNLGFVNSGEQ 553

BLAST of Cucsa.248400 vs. TrEMBL
Match: A0A151SJK0_CAJCA (Retrovirus-related Pol polyprotein from transposon TNT 1-94 (Fragment) OS=Cajanus cajan GN=KK1_001136 PE=4 SV=1)

HSP 1 Score: 241.9 bits (616), Expect = 1.7e-60
Identity = 158/497 (31.79%), Postives = 254/497 (51.11%), Query Frame = 1

Query: 5   KFQNHNLSEFLASKGIVHQNTCAYTPQQNGVAERKNYHLLEAARSRMLSTSLPSYLWGDT 64
           ++ +++L+  L   GI HQ++C +T QQN VAERKN H+L  ARS   +T++P++ WG+ 
Sbjct: 87  EYFSNDLNGDLQEHGIFHQSSCNHTLQQNRVAERKNRHILGVARSLKFTTNVPNHFWGEA 146

Query: 65  IITTTHLINRIPSRILHLQTPLDFLKESYPSTRLIYEVPLHVFGCTTYVHNFDPNQTKFT 124
           ++T T+LIN +PS+ L   TPL+ LK+ +P  R++  +P  +FGCT +VHN  P + K  
Sbjct: 147 VLTATYLINCLPSKPLQFLTPLNCLKDFFPLVRMLESIPPKIFGCTIFVHNSSPTRGKLD 206

Query: 125 HQAQTCVFVGYPLHQRGYKCFHPPSLQ---ELGRSLKKEVAFPTSQSSTAVQDSEP---- 184
            ++  C+F+GY   Q+GYKC+ P S +       +  +   F  + S   V   +P    
Sbjct: 207 PKSHQCIFLGYSPTQKGYKCYCPKSKRFYISCDTTFLENQPFFHNDSFQGVNMIKPHHWD 266

Query: 185 ---------PRDQGMENPTKSCTNNIISVNDLSNVVILE--NVEEKNSSDETEVGTKTSN 244
                    P  + ++  +++ +  I S+        +E  NVE  + + E     +  N
Sbjct: 267 PSISLPISLPLPEPIQKDSETKSTQITSLGGELEKRNMEPNNVEAVDCNTEGNCAFENLN 326

Query: 245 NE------------VEQGTRSYTKHPICNFVSYDNLSPQFRVFTATLDSTIIPKNIYTAL 304
            E            + +G RS TKH I NF++Y NLS ++R F   LD   IP  ++ AL
Sbjct: 327 VENDTIDEFDLPIALRKGVRSCTKHSISNFLTYFNLSSRYRAFVTKLDRVQIPNTVFDAL 386

Query: 305 EVDGTLDRHKARLVAKGFTQTYAIDYSETFSLDAKLNTIRVMLYVVVNKEKTDRKTTEAN 364
           +        K R V         ++  E  S+++ ++   V           D K+T   
Sbjct: 387 K------DKKWRAV--------VLEEMEHRSVESFVDADWV-------GSVEDSKSTRGY 446

Query: 365 TDSAWRGSIIDRKSTFGYCTFVWGNLVTWRIKKQSVVARSSAEAEYKAMNLGICKEIWLQ 424
               W                   NLVTWR KKQSV+ARSSAE E +A+  G+C+   ++
Sbjct: 447 YTKVWG------------------NLVTWRSKKQSVIARSSAEVECRAIAHGVCELTSIK 506

Query: 425 KVLSDLHQECETPLKLFCDNKAAISIANNHVQHDRTKHVEIDRYFIKERLDCGSICIPYI 472
           ++L DL    + P+KL+ D+K+AI+I +N VQHD+ KHV IDR FIK  ++ G+  + Y+
Sbjct: 507 RLLHDLFIPLQGPVKLYGDSKSAINIVHNPVQHDKMKHVRIDRNFIKSEMENGTFSLHYV 544

BLAST of Cucsa.248400 vs. TrEMBL
Match: A5AGT0_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_006541 PE=4 SV=1)

HSP 1 Score: 231.5 bits (589), Expect = 2.2e-57
Identity = 151/418 (36.12%), Postives = 205/418 (49.04%), Query Frame = 1

Query: 1   DNERKFQNHNLSEFLASKGIVHQNTCAYTPQQNGVAERKNYHLLEAARSRMLSTSLPSYL 60
           DN R + N  L EFLA +GIVH ++C  TPQQNG+AERKN HLLE ARS M S ++P   
Sbjct: 16  DNARDYFNSILGEFLAQEGIVHLSSCVDTPQQNGIAERKNRHLLEVARSLMFSMNVPKLF 75

Query: 61  WGDTIITTTHLINRIPSRILHLQTPLDFLKESYPSTRLIYEVPLHVFGCTTYVHNFDPNQ 120
           WG  ++T  +LINR+ SR+L  QTP   L +S+P+TRLI  VP  +FGC+ +VH    ++
Sbjct: 76  WGQAVLTAAYLINRMXSRVLKFQTPCQTLLKSFPTTRLISTVPPKIFGCSVFVHINQQHR 135

Query: 121 TKFTHQAQTCVFVGYPLHQRGYKCFHPPSLQELGRSLKKEVAFPTSQSST--AVQDSEPP 180
           +K   ++  C+F+GY  +Q+GYKC+ P + +           F  S   T    Q   P 
Sbjct: 136 SKLDPRSLKCIFLGYSSNQKGYKCYSPVTRK-----------FYNSMDVTFFETQPYYPK 195

Query: 181 RDQGMENPTKS--------------CTNNII---SVNDLSNVV-------ILENVEEKNS 240
            D   EN T+                T N I   S N   ++V       I E  EE+  
Sbjct: 196 NDIQGENSTQEYQFWDLESFSESPITTENHIPPESFNQPESIVDLWDKEHIQEETEERAL 255

Query: 241 SDETEVGTK---------------TSNNEVE-----------QGTRSYTKHPICNFVSYD 300
           S +T                    T ++E+E           +G RS T+HPI NF+SYD
Sbjct: 256 SQQTHEAKPGPNPSKLPGNNAPDGTXDSELENDILNMPIAWRKGVRSCTQHPIGNFISYD 315

Query: 301 NLSPQFRVFTATLDSTIIPKNIYTAL---------------------------------- 323
            LSP FR FT+++    +P+NI  A                                   
Sbjct: 316 KLSPTFRAFTSSITEIQVPQNIQEAFKYPKWKAAVDEEVRALEKNGTWEITDLPRGKKPV 375

BLAST of Cucsa.248400 vs. TrEMBL
Match: A5AGT0_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_006541 PE=4 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 2.0e-42
Identity = 91/180 (50.56%), Postives = 124/180 (68.89%), Query Frame = 1

Query: 313 IRVMLYVVVNK------EKTDRKTTEANTDSAWRGSIIDRKSTFGYCTFVWGNLVTWRIK 372
           IR++ Y+ +        ++T +K  E  + + W GS+ DR+ST  YC+FVWGNLVTWR K
Sbjct: 652 IRILRYLKMTPGKGLFFQRTTKKEIEIFSXADWAGSVTDRRSTSXYCSFVWGNLVTWRSK 711

Query: 373 KQSVVARSSAEAEYKAMNLGICKEIWLQKVLSDLHQECETPLKLFCDNKAAISIANNHVQ 432
           KQSVVARSSAEAE++AM  GIC+ IWL  +L +L    + P+ L+CDN+AAISIA N V 
Sbjct: 712 KQSVVARSSAEAEFRAMAQGICEGIWLNXLLEELRVSLKHPMVLYCDNQAAISIAKNPVH 771

Query: 433 HDRTKHVEIDRYFIKERLDCGSICIPYIHSSQQVADVLTNGLLIPNFDFYISKLRLIDIY 487
           HDRTKHVEIDR+FIKE+++ G   + Y  ++ Q AD+LT  L   NF+    KL +I+I+
Sbjct: 772 HDRTKHVEIDRHFIKEKIEEGVFKVSYTPTNCQTADILTKALARVNFEDLTEKLGMINIH 831


HSP 2 Score: 231.1 bits (588), Expect = 2.9e-57
Identity = 153/418 (36.60%), Postives = 208/418 (49.76%), Query Frame = 1

Query: 1   DNERKFQNHNLSEFLASKGIVHQNTCAYTPQQNGVAERKNYHLLEAARSRMLSTSLPSYL 60
           DN R + N  L EFLA +GIVH ++C  TPQQNG+AERKN HLLE ARS M S ++P   
Sbjct: 362 DNARDYFNSILGEFLAQEGIVHLSSCVDTPQQNGIAERKNRHLLEVARSLMFSMNVPKLF 421

Query: 61  WGDTIITTTHLINRIPSRILHLQTPLDFLKESYPSTRLIYEVPLHVFGCTTYVHNFDPNQ 120
            G  ++T  +LINR+PSR+L  QTP   L +S+P+TRLI  VP  +FGC+ +VH    ++
Sbjct: 422 XGQAVLTAAYLINRMPSRVLKFQTPCQTLLKSFPTTRLISTVPPKIFGCSXFVHINQQHR 481

Query: 121 TKFTHQAQTCVFVGYPLHQRGYKCFHPPSLQELGRSLKKEVAFPTSQSST--AVQDSEPP 180
           +K   ++  C+F+GY  +Q+GYKC+ P + +           F  S   T    Q   P 
Sbjct: 482 SKLDPRSLKCIFLGYSSNQKGYKCYSPVTRK-----------FYNSMDVTFFETQPYYPK 541

Query: 181 RDQGMENPTKS--------------CTNNII---SVNDLSNVV-------ILENVEEKNS 240
            D   EN T+                T N I   S N   ++V       I E  EE+  
Sbjct: 542 NDIQGENSTQEYQFWDLESFSESPITTENHIPPESFNQPESIVDLWDKEHIQEETEERXL 601

Query: 241 SDET---EVGTK------------TSNNEVE-----------QGTRSYTKHPICNFVSYD 300
           S +T   E G              T ++E+E           +G RS T+HPI NF+SYD
Sbjct: 602 SQQTHEAEPGPNPSKLPGNNAPDGTVDSELENDILNMPIAWRKGVRSCTQHPIGNFISYD 661

Query: 301 NLSPQFRVFTATLDSTIIPKNIYTAL---------------------------------- 323
            LSP FR FT+++    +P+NI+ A                                   
Sbjct: 662 KLSPTFRAFTSSITEIQVPQNIHEAFKYPKWKAAVDEEVRALEKNGTWEITDLPRGKKPV 721

BLAST of Cucsa.248400 vs. TrEMBL
Match: A5AQS6_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_006799 PE=4 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 7.5e-45
Identity = 94/180 (52.22%), Postives = 127/180 (70.56%), Query Frame = 1

Query: 313  IRVMLYVVVNK------EKTDRKTTEANTDSAWRGSIIDRKSTFGYCTFVWGNLVTWRIK 372
            IR++ Y+ +        ++T +K  E  +D+ W GS+ DR+ST GYC+FVWGNLVTWR K
Sbjct: 998  IRILRYLKMTPGKGLFFQRTTKKEIEIFSDADWAGSVTDRRSTSGYCSFVWGNLVTWRSK 1057

Query: 373  KQSVVARSSAEAEYKAMNLGICKEIWLQKVLSDLHQECETPLKLFCDNKAAISIANNHVQ 432
            KQSVVARSSAEAE++AM  GIC+ IWL ++L +L    + P+ L+CDN+AAISIA N V 
Sbjct: 1058 KQSVVARSSAEAEFRAMAQGICEGIWLNRLLEELRVPLKHPMVLYCDNQAAISIAKNPVH 1117

Query: 433  HDRTKHVEIDRYFIKERLDCGSICIPYIHSSQQVADVLTNGLLIPNFDFYISKLRLIDIY 487
            HDRTKHVEIDR+FIKE+++ G   + Y  ++ Q AD+LT  L   NF+    KL +I+IY
Sbjct: 1118 HDRTKHVEIDRHFIKEKIEEGVFKVSYTPTNCQTADILTKALARVNFEDLTEKLGMINIY 1177


HSP 2 Score: 230.3 bits (586), Expect = 5.0e-57
Identity = 146/416 (35.10%), Postives = 202/416 (48.56%), Query Frame = 1

Query: 1    DNERKFQNHNLSEFLASKGIVHQNTCAYTPQQNGVAERKNYHLLEAARSRMLSTSLPSYL 60
            DN R + N  L EFLA +GIVH ++C  TPQQNG+AERKN HLLE ARS M S ++P   
Sbjct: 758  DNARDYFNSILGEFLAQEGIVHLSSCVDTPQQNGIAERKNRHLLEVARSLMFSMNVPKLF 817

Query: 61   WGDTIITTTHLINRIPSRILHLQTPLDFLKESYPSTRLIYEVPLHVFGCTTYVHNFDPNQ 120
            WG  ++T  +LINR+P R+L  QTP   L +S+P+TRLI  VP  +FGC+ +VH    ++
Sbjct: 818  WGQAVLTAAYLINRMPXRVLKFQTPCQTLLKSFPTTRLISTVPPKIFGCSVFVHINQQHR 877

Query: 121  TKFTHQAQTCVFVGYPLHQRGYKCFHP--------------------PSLQELGRSLKKE 180
            +K   ++  C+F+GY  +Q+GYKC+ P                    P     G +   E
Sbjct: 878  SKXDPRSLKCIFLGYSSNQKGYKCYSPVTRKFYNSMDVTFFETXPYYPKNDIQGENSTXE 937

Query: 181  VAF----PTSQSSTAVQDSEPPRDQGMENPTKSCTNNIISVNDLSNVVILENVEEKNSSD 240
              F      S+S    ++  PP  +    P      +I+ + D  +  I E  EE+  S 
Sbjct: 938  YQFWDLESFSESPITTENHIPP--ESFNQP-----ESIVDLWDKEH--IQEETEERALSQ 997

Query: 241  ETEVG------TKTSNNEVEQGT--------------------RSYTKHPICNFVSYDNL 300
            +T         +K   N    GT                    +S T+HPI NF+SYD L
Sbjct: 998  QTHEAEPGPNPSKLPGNNAPDGTVDSELENDILNMPIAWRKEVKSCTQHPIGNFISYDKL 1057

Query: 301  SPQFRVFTATLDSTIIPKNIYTAL------------------------------------ 323
            SP FR FT+++    +P+NI  A                                     
Sbjct: 1058 SPTFRAFTSSITEIQVPQNIQEAFKYPKWKAAVDEEVRALEKNGTWEITDLPRGKKPVGC 1117

BLAST of Cucsa.248400 vs. TAIR10
Match: AT4G23160.1 (AT4G23160.1 cysteine-rich RLK (RECEPTOR-like protein kinase) 8)

HSP 1 Score: 102.1 bits (253), Expect = 1.0e-21
Identity = 48/109 (44.04%), Postives = 73/109 (66.97%), Query Frame = 1

Query: 335 TDSAWRGSIIDRKSTFGYCTFVWGNLVTWRIKKQSVVARSSAEAEYKAMNLGICKEIWLQ 394
           +D++++     R+ST GYC F+  +L++W+ KKQ VV++SSAEAEY+A++    + +WL 
Sbjct: 446 SDASFQSCKDTRRSTNGYCMFLGTSLISWKSKKQQVVSKSSAEAEYRALSFATDEMMWLA 505

Query: 395 KVLSDLHQECETPLKLFCDNKAAISIANNHVQHDRTKHVEIDRYFIKER 444
           +   +L      P  LFCDN AAI IA N V H+RTKH+E D + ++ER
Sbjct: 506 QFFRELQLPLSKPTLLFCDNTAAIHIATNAVFHERTKHIESDCHSVRER 554


HSP 2 Score: 51.6 bits (122), Expect = 1.6e-06
Identity = 23/43 (53.49%), Postives = 34/43 (79.07%), Query Frame = 1

Query: 277 DGTLDRHKARLVAKGFTQTYAIDYSETFSLDAKLNTIRVMLYV 320
           DGT++R+KARLVAKG+TQ   ID+ ETFS   KL +++++L +
Sbjct: 140 DGTIERYKARLVAKGYTQQEGIDFIETFSPVCKLTSVKLILAI 182

BLAST of Cucsa.248400 vs. TAIR10
Match: ATMG00810.1 (ATMG00810.1 DNA/RNA polymerases superfamily protein)

HSP 1 Score: 61.2 bits (147), Expect = 2.0e-09
Identity = 27/68 (39.71%), Postives = 40/68 (58.82%), Query Frame = 1

Query: 325 KTDRKTTEANTDSAWRGSIIDRKSTFGYCTFVWGNLVTWRIKKQSVVARSSAEAEYKAMN 384
           K  +   +A  DS W G    R+ST G+CTF+  N+++W  K+Q  V+RSS E EY+A+ 
Sbjct: 158 KNSKLNVQAFCDSDWAGCTSTRRSTTGFCTFLGCNIISWSAKRQPTVSRSSTETEYRALA 217

Query: 385 LGICKEIW 393
           L   +  W
Sbjct: 218 LTAAELTW 225

BLAST of Cucsa.248400 vs. NCBI nr
Match: gi|39545843|emb|CAE04751.3| (OSJNBb0060E08.14 [Oryza sativa Japonica Group])

HSP 1 Score: 244.2 bits (622), Expect = 4.8e-61
Identity = 159/477 (33.33%), Postives = 248/477 (51.99%), Query Frame = 1

Query: 20  IVHQNTCAYTPQQNGVAERKNYHLLEAARSRMLSTSLPSYLWGDTIITTTHLINRIPSRI 79
           +VH +        NGVAE KN H+LE  RS M + ++P +LW + +++ T+LINR+PSRI
Sbjct: 88  LVHSDVWTSPIASNGVAESKNRHILEVTRSLMYTMNVPKFLWSEAVMSATYLINRMPSRI 147

Query: 80  LHLQTPLDFLKESYPSTRLIYEVPLHVFGCTTYVHNFDPNQTKFTHQAQTCVFVGYPLHQ 139
           L ++TP + +   +     I  VP  VFGCT +V +  P+  K   +A  C+F+GY   Q
Sbjct: 148 LGMKTPYEMV---FGKNEFI--VPPKVFGCTCFVRDHRPSVGKLDPRAVKCIFIGYSSGQ 207

Query: 140 RGYKCFHPPSLQ---ELGRSLKKEVAF---PTSQSSTAVQDSEPPRDQ-GMENPTKSCTN 199
           +GYKC+ P   +    +  + ++ V F    T  SS  V    P  D+ G E    S  +
Sbjct: 208 KGYKCWSPSERRTFVSMDVTFRESVPFYGERTDLSSLFVDLDNPIIDEDGQEGENGSSGD 267

Query: 200 NIISVNDLSNVVILENVEEKNSSDETEVGTKTSNNEV--EQGTRSYT--KHPICNFVSYD 259
                +D  + +    +      +E E G + +N  +   +G RS    ++P  N   Y 
Sbjct: 268 K---PSDQCDTI---QISSDTEGEEFETGGEETNLPIAIRKGVRSNAVKQNPDGNVERYK 327

Query: 260 N---LSPQFRVFTATLDSTIIPKNIYTALEVD-----GTLDRHKARLVAKGFTQ------ 319
                    + +    D T  P    + L+V      G L       +  GF        
Sbjct: 328 ARLVAKGYSQTYGIDYDETFAPVPKMSTLDVKNAFLHGDLQEEVYMEIPPGFATSQTEDA 387

Query: 320 --TYAIDYSETFSLDAKLNTI----RVMLYVVVNK------EKTDRKTTEANTDSAWRGS 379
             TYA+     +  D +   +    R++ Y+  +       +K      E   D+ W   
Sbjct: 388 DITYAVSVVSRYMHDPRSGHMDVVYRILRYLKASPGKGIWFKKNGHLDVEGYCDADWGSC 447

Query: 380 IIDRKSTFGYCTFVWGNLVTWRIKKQSVVARSSAEAEYKAMNLGICKEIWLQKVLSDLHQ 439
           + D +ST GYC F+ GNLV+WR KKQSVV+RS+AEAEY++M++ + + +WL+ +L++L  
Sbjct: 448 LDDMRSTSGYCVFIGGNLVSWRSKKQSVVSRSTAEAEYRSMSMSLSELLWLKNLLAELKL 507

Query: 440 ECETPLKLFCDNKAAISIANNHVQHDRTKHVEIDRYFIKERLDCGSICIPYIHSSQQ 460
              T +KL+CDNK+AI+IANN VQHDRTKHVEIDR+FIKER+D G++ + +++S +Q
Sbjct: 508 STSTSMKLWCDNKSAINIANNPVQHDRTKHVEIDRFFIKERMDEGTLNLGFVNSGEQ 553

BLAST of Cucsa.248400 vs. NCBI nr
Match: gi|1012343743|gb|KYP54935.1| (Retrovirus-related Pol polyprotein from transposon TNT 1-94, partial [Cajanus cajan])

HSP 1 Score: 241.9 bits (616), Expect = 2.4e-60
Identity = 158/497 (31.79%), Postives = 254/497 (51.11%), Query Frame = 1

Query: 5   KFQNHNLSEFLASKGIVHQNTCAYTPQQNGVAERKNYHLLEAARSRMLSTSLPSYLWGDT 64
           ++ +++L+  L   GI HQ++C +T QQN VAERKN H+L  ARS   +T++P++ WG+ 
Sbjct: 87  EYFSNDLNGDLQEHGIFHQSSCNHTLQQNRVAERKNRHILGVARSLKFTTNVPNHFWGEA 146

Query: 65  IITTTHLINRIPSRILHLQTPLDFLKESYPSTRLIYEVPLHVFGCTTYVHNFDPNQTKFT 124
           ++T T+LIN +PS+ L   TPL+ LK+ +P  R++  +P  +FGCT +VHN  P + K  
Sbjct: 147 VLTATYLINCLPSKPLQFLTPLNCLKDFFPLVRMLESIPPKIFGCTIFVHNSSPTRGKLD 206

Query: 125 HQAQTCVFVGYPLHQRGYKCFHPPSLQ---ELGRSLKKEVAFPTSQSSTAVQDSEP---- 184
            ++  C+F+GY   Q+GYKC+ P S +       +  +   F  + S   V   +P    
Sbjct: 207 PKSHQCIFLGYSPTQKGYKCYCPKSKRFYISCDTTFLENQPFFHNDSFQGVNMIKPHHWD 266

Query: 185 ---------PRDQGMENPTKSCTNNIISVNDLSNVVILE--NVEEKNSSDETEVGTKTSN 244
                    P  + ++  +++ +  I S+        +E  NVE  + + E     +  N
Sbjct: 267 PSISLPISLPLPEPIQKDSETKSTQITSLGGELEKRNMEPNNVEAVDCNTEGNCAFENLN 326

Query: 245 NE------------VEQGTRSYTKHPICNFVSYDNLSPQFRVFTATLDSTIIPKNIYTAL 304
            E            + +G RS TKH I NF++Y NLS ++R F   LD   IP  ++ AL
Sbjct: 327 VENDTIDEFDLPIALRKGVRSCTKHSISNFLTYFNLSSRYRAFVTKLDRVQIPNTVFDAL 386

Query: 305 EVDGTLDRHKARLVAKGFTQTYAIDYSETFSLDAKLNTIRVMLYVVVNKEKTDRKTTEAN 364
           +        K R V         ++  E  S+++ ++   V           D K+T   
Sbjct: 387 K------DKKWRAV--------VLEEMEHRSVESFVDADWV-------GSVEDSKSTRGY 446

Query: 365 TDSAWRGSIIDRKSTFGYCTFVWGNLVTWRIKKQSVVARSSAEAEYKAMNLGICKEIWLQ 424
               W                   NLVTWR KKQSV+ARSSAE E +A+  G+C+   ++
Sbjct: 447 YTKVWG------------------NLVTWRSKKQSVIARSSAEVECRAIAHGVCELTSIK 506

Query: 425 KVLSDLHQECETPLKLFCDNKAAISIANNHVQHDRTKHVEIDRYFIKERLDCGSICIPYI 472
           ++L DL    + P+KL+ D+K+AI+I +N VQHD+ KHV IDR FIK  ++ G+  + Y+
Sbjct: 507 RLLHDLFIPLQGPVKLYGDSKSAINIVHNPVQHDKMKHVRIDRNFIKSEMENGTFSLHYV 544

BLAST of Cucsa.248400 vs. NCBI nr
Match: gi|147767923|emb|CAN73399.1| (hypothetical protein VITISV_006541 [Vitis vinifera])

HSP 1 Score: 231.5 bits (589), Expect = 3.2e-57
Identity = 151/418 (36.12%), Postives = 205/418 (49.04%), Query Frame = 1

Query: 1   DNERKFQNHNLSEFLASKGIVHQNTCAYTPQQNGVAERKNYHLLEAARSRMLSTSLPSYL 60
           DN R + N  L EFLA +GIVH ++C  TPQQNG+AERKN HLLE ARS M S ++P   
Sbjct: 16  DNARDYFNSILGEFLAQEGIVHLSSCVDTPQQNGIAERKNRHLLEVARSLMFSMNVPKLF 75

Query: 61  WGDTIITTTHLINRIPSRILHLQTPLDFLKESYPSTRLIYEVPLHVFGCTTYVHNFDPNQ 120
           WG  ++T  +LINR+ SR+L  QTP   L +S+P+TRLI  VP  +FGC+ +VH    ++
Sbjct: 76  WGQAVLTAAYLINRMXSRVLKFQTPCQTLLKSFPTTRLISTVPPKIFGCSVFVHINQQHR 135

Query: 121 TKFTHQAQTCVFVGYPLHQRGYKCFHPPSLQELGRSLKKEVAFPTSQSST--AVQDSEPP 180
           +K   ++  C+F+GY  +Q+GYKC+ P + +           F  S   T    Q   P 
Sbjct: 136 SKLDPRSLKCIFLGYSSNQKGYKCYSPVTRK-----------FYNSMDVTFFETQPYYPK 195

Query: 181 RDQGMENPTKS--------------CTNNII---SVNDLSNVV-------ILENVEEKNS 240
            D   EN T+                T N I   S N   ++V       I E  EE+  
Sbjct: 196 NDIQGENSTQEYQFWDLESFSESPITTENHIPPESFNQPESIVDLWDKEHIQEETEERAL 255

Query: 241 SDETEVGTK---------------TSNNEVE-----------QGTRSYTKHPICNFVSYD 300
           S +T                    T ++E+E           +G RS T+HPI NF+SYD
Sbjct: 256 SQQTHEAKPGPNPSKLPGNNAPDGTXDSELENDILNMPIAWRKGVRSCTQHPIGNFISYD 315

Query: 301 NLSPQFRVFTATLDSTIIPKNIYTAL---------------------------------- 323
            LSP FR FT+++    +P+NI  A                                   
Sbjct: 316 KLSPTFRAFTSSITEIQVPQNIQEAFKYPKWKAAVDEEVRALEKNGTWEITDLPRGKKPV 375

BLAST of Cucsa.248400 vs. NCBI nr
Match: gi|147767923|emb|CAN73399.1| (hypothetical protein VITISV_006541 [Vitis vinifera])

HSP 1 Score: 181.8 bits (460), Expect = 2.9e-42
Identity = 91/180 (50.56%), Postives = 124/180 (68.89%), Query Frame = 1

Query: 313 IRVMLYVVVNK------EKTDRKTTEANTDSAWRGSIIDRKSTFGYCTFVWGNLVTWRIK 372
           IR++ Y+ +        ++T +K  E  + + W GS+ DR+ST  YC+FVWGNLVTWR K
Sbjct: 652 IRILRYLKMTPGKGLFFQRTTKKEIEIFSXADWAGSVTDRRSTSXYCSFVWGNLVTWRSK 711

Query: 373 KQSVVARSSAEAEYKAMNLGICKEIWLQKVLSDLHQECETPLKLFCDNKAAISIANNHVQ 432
           KQSVVARSSAEAE++AM  GIC+ IWL  +L +L    + P+ L+CDN+AAISIA N V 
Sbjct: 712 KQSVVARSSAEAEFRAMAQGICEGIWLNXLLEELRVSLKHPMVLYCDNQAAISIAKNPVH 771

Query: 433 HDRTKHVEIDRYFIKERLDCGSICIPYIHSSQQVADVLTNGLLIPNFDFYISKLRLIDIY 487
           HDRTKHVEIDR+FIKE+++ G   + Y  ++ Q AD+LT  L   NF+    KL +I+I+
Sbjct: 772 HDRTKHVEIDRHFIKEKIEEGVFKVSYTPTNCQTADILTKALARVNFEDLTEKLGMINIH 831


HSP 2 Score: 231.1 bits (588), Expect = 4.2e-57
Identity = 153/418 (36.60%), Postives = 208/418 (49.76%), Query Frame = 1

Query: 1   DNERKFQNHNLSEFLASKGIVHQNTCAYTPQQNGVAERKNYHLLEAARSRMLSTSLPSYL 60
           DN R + N  L EFLA +GIVH ++C  TPQQNG+AERKN HLLE ARS M S ++P   
Sbjct: 362 DNARDYFNSILGEFLAQEGIVHLSSCVDTPQQNGIAERKNRHLLEVARSLMFSMNVPKLF 421

Query: 61  WGDTIITTTHLINRIPSRILHLQTPLDFLKESYPSTRLIYEVPLHVFGCTTYVHNFDPNQ 120
            G  ++T  +LINR+PSR+L  QTP   L +S+P+TRLI  VP  +FGC+ +VH    ++
Sbjct: 422 XGQAVLTAAYLINRMPSRVLKFQTPCQTLLKSFPTTRLISTVPPKIFGCSXFVHINQQHR 481

Query: 121 TKFTHQAQTCVFVGYPLHQRGYKCFHPPSLQELGRSLKKEVAFPTSQSST--AVQDSEPP 180
           +K   ++  C+F+GY  +Q+GYKC+ P + +           F  S   T    Q   P 
Sbjct: 482 SKLDPRSLKCIFLGYSSNQKGYKCYSPVTRK-----------FYNSMDVTFFETQPYYPK 541

Query: 181 RDQGMENPTKS--------------CTNNII---SVNDLSNVV-------ILENVEEKNS 240
            D   EN T+                T N I   S N   ++V       I E  EE+  
Sbjct: 542 NDIQGENSTQEYQFWDLESFSESPITTENHIPPESFNQPESIVDLWDKEHIQEETEERXL 601

Query: 241 SDET---EVGTK------------TSNNEVE-----------QGTRSYTKHPICNFVSYD 300
           S +T   E G              T ++E+E           +G RS T+HPI NF+SYD
Sbjct: 602 SQQTHEAEPGPNPSKLPGNNAPDGTVDSELENDILNMPIAWRKGVRSCTQHPIGNFISYD 661

Query: 301 NLSPQFRVFTATLDSTIIPKNIYTAL---------------------------------- 323
            LSP FR FT+++    +P+NI+ A                                   
Sbjct: 662 KLSPTFRAFTSSITEIQVPQNIHEAFKYPKWKAAVDEEVRALEKNGTWEITDLPRGKKPV 721

BLAST of Cucsa.248400 vs. NCBI nr
Match: gi|147856541|emb|CAN82483.1| (hypothetical protein VITISV_006799 [Vitis vinifera])

HSP 1 Score: 189.9 bits (481), Expect = 1.1e-44
Identity = 94/180 (52.22%), Postives = 127/180 (70.56%), Query Frame = 1

Query: 313  IRVMLYVVVNK------EKTDRKTTEANTDSAWRGSIIDRKSTFGYCTFVWGNLVTWRIK 372
            IR++ Y+ +        ++T +K  E  +D+ W GS+ DR+ST GYC+FVWGNLVTWR K
Sbjct: 998  IRILRYLKMTPGKGLFFQRTTKKEIEIFSDADWAGSVTDRRSTSGYCSFVWGNLVTWRSK 1057

Query: 373  KQSVVARSSAEAEYKAMNLGICKEIWLQKVLSDLHQECETPLKLFCDNKAAISIANNHVQ 432
            KQSVVARSSAEAE++AM  GIC+ IWL ++L +L    + P+ L+CDN+AAISIA N V 
Sbjct: 1058 KQSVVARSSAEAEFRAMAQGICEGIWLNRLLEELRVPLKHPMVLYCDNQAAISIAKNPVH 1117

Query: 433  HDRTKHVEIDRYFIKERLDCGSICIPYIHSSQQVADVLTNGLLIPNFDFYISKLRLIDIY 487
            HDRTKHVEIDR+FIKE+++ G   + Y  ++ Q AD+LT  L   NF+    KL +I+IY
Sbjct: 1118 HDRTKHVEIDRHFIKEKIEEGVFKVSYTPTNCQTADILTKALARVNFEDLTEKLGMINIY 1177


HSP 2 Score: 230.7 bits (587), Expect = 5.5e-57
Identity = 146/416 (35.10%), Postives = 202/416 (48.56%), Query Frame = 1

Query: 1    DNERKFQNHNLSEFLASKGIVHQNTCAYTPQQNGVAERKNYHLLEAARSRMLSTSLPSYL 60
            DN R + N  L EFLA +GIVH ++C  TPQQNG+AERKN HLLE ARS M S ++P   
Sbjct: 758  DNARDYFNSILGEFLAQEGIVHLSSCVDTPQQNGIAERKNRHLLEVARSLMFSMNVPKLF 817

Query: 61   WGDTIITTTHLINRIPSRILHLQTPLDFLKESYPSTRLIYEVPLHVFGCTTYVHNFDPNQ 120
            WG  ++T  +LINR+P R+L  QTP   L +S+P+TRLI  VP  +FGC+ +VH    ++
Sbjct: 818  WGQAVLTAAYLINRMPXRVLKFQTPCQTLLKSFPTTRLISTVPPKIFGCSVFVHINQQHR 877

Query: 121  TKFTHQAQTCVFVGYPLHQRGYKCFHP--------------------PSLQELGRSLKKE 180
            +K   ++  C+F+GY  +Q+GYKC+ P                    P     G +   E
Sbjct: 878  SKJDPRSLKCIFLGYSSNQKGYKCYSPVTRKFYNSMDVTFFETXPYYPKNDIQGENSTXE 937

Query: 181  VAF----PTSQSSTAVQDSEPPRDQGMENPTKSCTNNIISVNDLSNVVILENVEEKNSSD 240
              F      S+S    ++  PP  +    P      +I+ + D  +  I E  EE+  S 
Sbjct: 938  YQFWDLESFSESPITTENHIPP--ESFNQP-----ESIVDLWDKEH--IQEETEERALSQ 997

Query: 241  ETEVG------TKTSNNEVEQGT--------------------RSYTKHPICNFVSYDNL 300
            +T         +K   N    GT                    +S T+HPI NF+SYD L
Sbjct: 998  QTHEAEPGPNPSKLPGNNAPDGTVDSELENDILNMPIAWRKEVKSCTQHPIGNFISYDKL 1057

Query: 301  SPQFRVFTATLDSTIIPKNIYTAL------------------------------------ 323
            SP FR FT+++    +P+NI  A                                     
Sbjct: 1058 SPTFRAFTSSITEIQVPQNIQEAFKYPKWKAAVDEEVRALEKNGTWEITDLPRGKKPVGC 1117

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
COPIA_DROME2.1e-2440.94Copia protein OS=Drosophila melanogaster GN=GIP PE=1 SV=3[more]
POLX_TOBAC7.0e-2029.46Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum... [more]
M810_ARATH3.6e-0839.71Uncharacterized mitochondrial protein AtMg00810 OS=Arabidopsis thaliana GN=AtMg0... [more]
Match NameE-valueIdentityDescription
Q7XM85_ORYSJ3.3e-6133.33OSJNBb0060E08.14 protein OS=Oryza sativa subsp. japonica GN=OSJNBb0060E08.14 PE=... [more]
A0A151SJK0_CAJCA1.7e-6031.79Retrovirus-related Pol polyprotein from transposon TNT 1-94 (Fragment) OS=Cajanu... [more]
A5AGT0_VITVI2.2e-5736.12Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_006541 PE=4 SV=1[more]
A5AGT0_VITVI2.0e-4250.56Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_006541 PE=4 SV=1[more]
A5AQS6_VITVI7.5e-4552.22Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_006799 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G23160.11.0e-2144.04 cysteine-rich RLK (RECEPTOR-like protein kinase) 8[more]
ATMG00810.12.0e-0939.71ATMG00810.1 DNA/RNA polymerases superfamily protein[more]
Match NameE-valueIdentityDescription
gi|39545843|emb|CAE04751.3|4.8e-6133.33OSJNBb0060E08.14 [Oryza sativa Japonica Group][more]
gi|1012343743|gb|KYP54935.1|2.4e-6031.79Retrovirus-related Pol polyprotein from transposon TNT 1-94, partial [Cajanus ca... [more]
gi|147767923|emb|CAN73399.1|3.2e-5736.12hypothetical protein VITISV_006541 [Vitis vinifera][more]
gi|147767923|emb|CAN73399.1|2.9e-4250.56hypothetical protein VITISV_006541 [Vitis vinifera][more]
gi|147856541|emb|CAN82483.1|1.1e-4452.22hypothetical protein VITISV_006799 [Vitis vinifera][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001584Integrase_cat-core
IPR012337RNaseH-like_sf
IPR013103RVT_2
Vocabulary: Biological Process
TermDefinition
GO:0015074DNA integration
Vocabulary: Molecular Function
TermDefinition
GO:0003676nucleic acid binding
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015074 DNA integration
cellular_component GO:0005575 cellular_component
molecular_function GO:0003676 nucleic acid binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.248400.1Cucsa.248400.1mRNA


Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001584Integrase, catalytic corePFAMPF00665rvecoord: 1..43
score: 2.
IPR001584Integrase, catalytic corePROFILEPS50994INTEGRASEcoord: 1..93
score: 13
IPR012337Ribonuclease H-like domainGENE3DG3DSA:3.30.420.10coord: 1..85
score: 4.9
IPR012337Ribonuclease H-like domainunknownSSF53098Ribonuclease H-likecoord: 1..97
score: 1.37
IPR013103Reverse transcriptase, RNA-dependent DNA polymerasePFAMPF07727RVT_2coord: 270..324
score: 5.5
NoneNo IPR availablePANTHERPTHR11439GAG-POL-RELATED RETROTRANSPOSONcoord: 1..408
score: 4.4
NoneNo IPR availablePANTHERPTHR11439:SF185SUBFAMILY NOT NAMEDcoord: 1..408
score: 4.4

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None