BLAST of Cucsa.242290 vs. Swiss-Prot
Match:
GCP2_ARATH (Probable glutamate carboxypeptidase 2 OS=Arabidopsis thaliana GN=AMP1 PE=1 SV=3)
HSP 1 Score: 775.4 bits (2001), Expect = 5.2e-223
Identity = 398/697 (57.10%), Postives = 504/697 (72.31%), Query Frame = 1
Query: 16 RPAPLPTFFFVIIICVLGFYTFHFSTSSSFSLL---------------LSSGSNYTVASY 75
+P PL +F FVI++ V FYT H + + LL LSS SN T++SY
Sbjct: 21 QPPPLCSFLFVIVLFVATFYTLHHPDAVTPPLLFSRNAYNALRLRRLFLSSASNATISSY 80
Query: 76 LRSLTLHPHLAGTEPSSETVRYVESHFRDLGLETHSIQYDALLSYPKSTSLSVLLSNGTV 135
LR LT HPHLAGT+PS +T+ YV +HF+ LGLETH +Y+ALLSYP S++ SN T
Sbjct: 81 LRELTRHPHLAGTKPSLDTLHYVFNHFQSLGLETHVAEYEALLSYPTHISVTASFSNTTT 140
Query: 136 VNIPLSENVEG---VVQPYHAYSPSGTAYGPAVFVNYGRDEDYRELAKMGVTVVGCIAVA 195
+ L++ V G VV+PYHAYSPSG+A G VFVN+G + DY L +GV+V GC+ +A
Sbjct: 141 LEFDLND-VPGDSPVVRPYHAYSPSGSAQGNVVFVNHGEERDYHALESIGVSVKGCVVLA 200
Query: 196 RKGE-FPRGVVVAKAEANGAKGVLLYVEGDGFR-QGFERGTVMRGIGDPLSPGWAAVDGA 255
RKGE RG +V AEA GA GVL+Y E DG G ERGTVMRGIGDP+SPGW V G
Sbjct: 201 RKGENLGRGAIVKIAEAKGALGVLIYAENDGGGFGGIERGTVMRGIGDPVSPGWPGVVGG 260
Query: 256 ERLNLNDSEVLKRFPKIPSMPLSAESAEIILSSLDTASVPPEWRDKKANLGSAAVGPG-- 315
E+L+L+D V +RFPKIPS+PLS +AEIIL+SL A P EWR+ S VGPG
Sbjct: 261 EKLSLDDELVTRRFPKIPSLPLSLRNAEIILASLGGARAPLEWRN------SGRVGPGQR 320
Query: 316 ---GPIFINFTYQGERKVATIRNVIAVIKGLEEPDRFVLMGNHRDAWSFGAVDPNSGTAA 375
G + IN T+QGE K+ I NV+ I+G EE DR+V++GNHRDAW++GAVDPNSGT+A
Sbjct: 321 VGPGRMVINMTFQGEMKMKKINNVVVTIRGSEEADRYVILGNHRDAWTYGAVDPNSGTSA 380
Query: 376 LLDIARRFSLLRRLGWNPRRTILLCSWDAEEFGMIGSTEWVEQNIVNLGTKAVAYLNVDC 435
LLDI+RRF+LL + GW PRRTILLCSWDAEEFGMIGSTEW+E+N++NLG AVAYLNVDC
Sbjct: 381 LLDISRRFALLLKSGWRPRRTILLCSWDAEEFGMIGSTEWIEENVLNLGASAVAYLNVDC 440
Query: 436 AVQGPGFFAGATPQLDDLLHDVTAQVQDPDVKGATVHDTWTAENGIGNIERLGAVNSDFA 495
AVQG GFFAGATPQLD LL DV VQDPD G TV +T+ ++N I I+RL V+SDF+
Sbjct: 441 AVQGSGFFAGATPQLDGLLVDVLKLVQDPDAVGLTVEETFKSQNNI--IQRLSRVDSDFS 500
Query: 496 AFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPLFHRHVTVGSIWGLLALRLSDD 555
F+ HAG+PS+D+YYG D+PVYHTAFD+YDWM + DPLFHRHV + IWGLL + L+D+
Sbjct: 501 GFLHHAGIPSIDMYYGADYPVYHTAFDSYDWMIHNADPLFHRHVAMAGIWGLLGILLADE 560
Query: 556 LILPFSYISYANQLQAYKDTLNHLLDGSVSLHSLSSSIEELKFAAQEIENEAKRLREQEA 615
++PF YISYA+QLQA++D L+ LL+G VS++ LS +I+E A+E +EAK+L+ +
Sbjct: 561 PLIPFDYISYADQLQAHRDKLSKLLEGKVSVNPLSMAIQEFSLVAKEAADEAKKLKGKSY 620
Query: 616 SSD--VALFQKRALNDRLMLAERGFLDVDGLRGHPWFKHLVYGPLSNYESALVYFPGIAD 675
S + A ++R LNDRLML ERGFLD +G++G WFKHLVYGP + ES L +FPGIAD
Sbjct: 621 SKNDVAAAAKRRELNDRLMLVERGFLDAEGIKGKEWFKHLVYGPAAEPESKLGFFPGIAD 680
Query: 676 AVSESKEINKRELEELIQHEIWRVARAIRRAAAALKG 686
A++ + E +I+HEIWRVARAI+RA+ ALKG
Sbjct: 681 AIAMNAS------EGIIEHEIWRVARAIQRASKALKG 702
BLAST of Cucsa.242290 vs. Swiss-Prot
Match:
FOLH1_RAT (Glutamate carboxypeptidase 2 OS=Rattus norvegicus GN=Folh1 PE=1 SV=1)
HSP 1 Score: 362.5 bits (929), Expect = 1.1e-98
Identity = 234/691 (33.86%), Postives = 372/691 (53.84%), Query Frame = 1
Query: 57 VASYLRSLTLHPHLAGTEPSSETVRYVESHFRDLGLETHSIQ-YDALLSYPKSTS---LS 116
+ +L + T PHLAGT+ + E + + + +++ GL+ + YD LLSYP T +S
Sbjct: 72 IKKFLYNFTRTPHLAGTQHNFELAKQIHAQWKEFGLDLVELSDYDVLLSYPNKTHPNYIS 131
Query: 117 VLLSNGTVV--------NIPLSENVEGVVQPYHAYSPSGTAYGPAVFVNYGRDEDYRELA 176
++ +G + + P EN+ VV PY A+SP GT G V+VNY R ED+ +L
Sbjct: 132 IINEDGNEIFKTSLAELSPPGYENISDVVPPYSAFSPQGTPEGDLVYVNYARTEDFFKLE 191
Query: 177 K-MGVTVVGCIAVARKGEFPRGVVVAKAEANGAKGVLLYVEG------------DGFRQ- 236
+ M + G I +AR G+ RG V A+ GAKG++LY + DG+
Sbjct: 192 RVMKINCSGKIVIARYGQVFRGNKVKNAQLAGAKGIILYSDPADYFVPGVKSYPDGWNLP 251
Query: 237 --GFERGTVMR--GIGDPLSPGWAAVDGAERLNLNDSEVLKRFPKIPSMPLSAESAEIIL 296
G +RG V+ G GDPL+PG+ A + A R ++ L P IP P+ + A+ +L
Sbjct: 252 GGGVQRGNVLNLNGAGDPLTPGYPANEYAYRHEFTEAVGL---PSIPVHPIGYDDAQKLL 311
Query: 297 SSLDTASVPPEWRDKKANLGSAAVGPGGPIFINFTYQGER-------KVATIRNVIAVIK 356
+ S PP+ K VGPG NF+ Q + KV I NVI +K
Sbjct: 312 EHMG-GSAPPDSSWKGGLKVPYNVGPG--FAGNFSKQKVKLHIHSYNKVTRIYNVIGTLK 371
Query: 357 GLEEPDRFVLMGNHRDAWSFGAVDPNSGTAALLDIARRFSLLRRLGWNPRRTILLCSWDA 416
G EPDR+V++G HRDAW FG +DP SG A + +I R F L++ GW PRRTIL SWDA
Sbjct: 372 GAVEPDRYVILGGHRDAWVFGGIDPQSGAAVVHEIVRTFGTLKKKGWRPRRTILFASWDA 431
Query: 417 EEFGMIGSTEWVEQNIVNLGTKAVAYLNVDCAVQGP-GFFAGATPQLDDLLHDVTAQVQD 476
EEFG++GSTEW E++ L + VAY+N D +++G TP + L++++T ++
Sbjct: 432 EEFGLLGSTEWAEEHSRLLQERGVAYINADSSIEGNYTLRVDCTPLMHSLVYNLTKELPS 491
Query: 477 PD--VKGATVHDTWTAEN------GIGNIERLGAVNSDFAAFVQHAGVPSVDVYYGRD-- 536
PD +G +++D+W ++ G+ I +LG+ N DF F Q G+ S Y ++
Sbjct: 492 PDEGFEGKSLYDSWKEKSPSTEFIGMPRISKLGSGN-DFEVFFQRLGIASGRARYTKNWK 551
Query: 537 ------FPVYHTAFDTYDWMANYGDPLFHRHVTVGSIWGLLALRLSDDLILPFSYISYAN 596
+P+YH+ ++TY+ + + DP F H+TV + G + L++ ++LPF SYA
Sbjct: 552 NNKVSSYPLYHSVYETYELVEKFYDPTFKYHLTVAQVRGAMVFELANSIVLPFDCQSYAV 611
Query: 597 QLQAYKDT-----LNH---LLDGSVSLHSLSSSIEELKFAAQEIENEAKRLREQEASSDV 656
L+ + +T +NH + +S SL S++ A + +RL++ + S+ +
Sbjct: 612 ALKKHAETIYNISMNHPQEMKAYMISFDSLFSAVNNFTDVASKFN---QRLQDLDKSNPI 671
Query: 657 ALFQKRALNDRLMLAERGFLDVDGLRGHPWFKHLVYGPLSNYESALVYFPGIADAVSE-S 685
L R LND+LM ER F+D GL G P+++H++Y P S+ + A FPGI DA+ + +
Sbjct: 672 LL---RILNDQLMYLERAFIDPLGLPGRPFYRHIIYAPSSHNKYAGESFPGIYDALFDIN 731
BLAST of Cucsa.242290 vs. Swiss-Prot
Match:
FOLH1_MOUSE (Glutamate carboxypeptidase 2 OS=Mus musculus GN=Folh1 PE=1 SV=2)
HSP 1 Score: 361.3 bits (926), Expect = 2.3e-98
Identity = 235/691 (34.01%), Postives = 369/691 (53.40%), Query Frame = 1
Query: 57 VASYLRSLTLHPHLAGTEPSSETVRYVESHFRDLGLETHSIQ-YDALLSYPKSTS---LS 116
+ +L + T PHLAGT+ + E + + +++ GL+ + YD LLSYP T +S
Sbjct: 72 IKKFLYNFTRTPHLAGTQNNFELAKQIHDQWKEFGLDLVELSHYDVLLSYPNKTHPNYIS 131
Query: 117 VLLSNGTVV--------NIPLSENVEGVVQPYHAYSPSGTAYGPAVFVNYGRDEDYRELA 176
++ +G + P EN+ VV PY A+SP GT G V+VNY R ED+ +L
Sbjct: 132 IINEDGNEIFKTSLSEQPPPGYENISDVVPPYSAFSPQGTPEGDLVYVNYARTEDFFKLE 191
Query: 177 K-MGVTVVGCIAVARKGEFPRGVVVAKAEANGAKGVLLYVEG------------DGFRQ- 236
+ M ++ G I +AR G+ RG +V A+ GAKG++LY + DG+
Sbjct: 192 REMKISCSGKIVIARYGKVFRGNMVKNAQLAGAKGMILYSDPADYFVPAVKSYPDGWNLP 251
Query: 237 --GFERGTVMR--GIGDPLSPGWAAVDGAERLNLNDSEVLKRFPKIPSMPLSAESAEIIL 296
G +RG V+ G GDPL+PG+ A + A R L ++ L P IP P+ + A+ +L
Sbjct: 252 GGGVQRGNVLNLNGAGDPLTPGYPANEHAYRHELTNAVGL---PSIPVHPIGYDDAQKLL 311
Query: 297 SSLDTASVPPEWRDKKANLGSAAVGPGGPIFINFTYQGER-------KVATIRNVIAVIK 356
+ PP+ K VGPG NF+ Q + KV I NVI +K
Sbjct: 312 EHMG-GPAPPDSSWKGGLKVPYNVGPG--FAGNFSTQKVKMHIHSYTKVTRIYNVIGTLK 371
Query: 357 GLEEPDRFVLMGNHRDAWSFGAVDPNSGTAALLDIARRFSLLRRLGWNPRRTILLCSWDA 416
G EPDR+V++G HRDAW FG +DP SG A + +I R F L++ G PRRTIL SWDA
Sbjct: 372 GALEPDRYVILGGHRDAWVFGGIDPQSGAAVVHEIVRSFGTLKKKGRRPRRTILFASWDA 431
Query: 417 EEFGMIGSTEWVEQNIVNLGTKAVAYLNVDCAVQGP-GFFAGATPQLDDLLHDVTAQVQD 476
EEFG++GSTEW E++ L + VAY+N D +++G TP + L++++T ++Q
Sbjct: 432 EEFGLLGSTEWAEEHSRLLQERGVAYINADSSIEGNYTLRVDCTPLMYSLVYNLTKELQS 491
Query: 477 PD--VKGATVHDTWTAEN------GIGNIERLGAVNSDFAAFVQHAGVPSVDVYYGRD-- 536
PD +G +++D+W ++ G+ I +LG+ N DF F Q G+ S Y ++
Sbjct: 492 PDEGFEGKSLYDSWKEKSPSPEFIGMPRISKLGSGN-DFEVFFQRLGIASGRARYTKNWK 551
Query: 537 ------FPVYHTAFDTYDWMANYGDPLFHRHVTVGSIWGLLALRLSDDLILPFSYISYAN 596
+P+YH+ ++TY+ + + DP F H+TV + G + L++ ++LPF SYA
Sbjct: 552 TNKVSSYPLYHSVYETYELVVKFYDPTFKYHLTVAQVRGAMVFELANSIVLPFDCQSYAV 611
Query: 597 QLQAYKDTL--------NHLLDGSVSLHSLSSSIEELKFAAQEIENEAKRLREQEASSDV 656
L+ Y DT+ + +S SL S++ A + +RL+E + S+ +
Sbjct: 612 ALKKYADTIYNISMKHPQEMKAYMISFDSLFSAVNNFTDVASKFN---QRLQELDKSNPI 671
Query: 657 ALFQKRALNDRLMLAERGFLDVDGLRGHPWFKHLVYGPLSNYESALVYFPGIADAVSE-S 685
L R +ND+LM ER F+D GL G P+++H++Y P S+ + A FPGI DA+ + S
Sbjct: 672 LL---RIMNDQLMYLERAFIDPLGLPGRPFYRHIIYAPSSHNKYAGESFPGIYDALFDIS 731
BLAST of Cucsa.242290 vs. Swiss-Prot
Match:
FOLH1_HUMAN (Glutamate carboxypeptidase 2 OS=Homo sapiens GN=FOLH1 PE=1 SV=1)
HSP 1 Score: 359.8 bits (922), Expect = 6.8e-98
Identity = 227/688 (32.99%), Postives = 369/688 (53.63%), Query Frame = 1
Query: 57 VASYLRSLTLHPHLAGTEPSSETVRYVESHFRDLGLETHSI-QYDALLSYPKSTS---LS 116
+ +L + T PHLAGTE + + + ++S +++ GL++ + YD LLSYP T +S
Sbjct: 70 IKKFLYNFTQIPHLAGTEQNFQLAKQIQSQWKEFGLDSVELAHYDVLLSYPNKTHPNYIS 129
Query: 117 VLLSNGTVV--------NIPLSENVEGVVQPYHAYSPSGTAYGPAVFVNYGRDEDYRELA 176
++ +G + P ENV +V P+ A+SP G G V+VNY R ED+ +L
Sbjct: 130 IINEDGNEIFNTSLFEPPPPGYENVSDIVPPFSAFSPQGMPEGDLVYVNYARTEDFFKLE 189
Query: 177 K-MGVTVVGCIAVARKGEFPRGVVVAKAEANGAKGVLLYVEG------------DGFRQ- 236
+ M + G I +AR G+ RG V A+ GAKGV+LY + DG+
Sbjct: 190 RDMKINCSGKIVIARYGKVFRGNKVKNAQLAGAKGVILYSDPADYFAPGVKSYPDGWNLP 249
Query: 237 --GFERGTVMR--GIGDPLSPGWAAVDGAERLNLNDSEVLKRFPKIPSMPLSAESAEIIL 296
G +RG ++ G GDPL+PG+ A + A R + ++ L P IP P+ A+ +L
Sbjct: 250 GGGVQRGNILNLNGAGDPLTPGYPANEYAYRRGIAEAVGL---PSIPVHPIGYYDAQKLL 309
Query: 297 SSLDTASVPPE--WRDKKA---NLGSAAVGPGGPIFINFTYQGERKVATIRNVIAVIKGL 356
+ S PP+ WR N+G G + +V I NVI ++G
Sbjct: 310 EKMG-GSAPPDSSWRGSLKVPYNVGPGFTGNFSTQKVKMHIHSTNEVTRIYNVIGTLRGA 369
Query: 357 EEPDRFVLMGNHRDAWSFGAVDPNSGTAALLDIARRFSLLRRLGWNPRRTILLCSWDAEE 416
EPDR+V++G HRD+W FG +DP SG A + +I R F L++ GW PRRTIL SWDAEE
Sbjct: 370 VEPDRYVILGGHRDSWVFGGIDPQSGAAVVHEIVRSFGTLKKEGWRPRRTILFASWDAEE 429
Query: 417 FGMIGSTEWVEQNIVNLGTKAVAYLNVDCAVQGP-GFFAGATPQLDDLLHDVTAQVQDPD 476
FG++GSTEW E+N L + VAY+N D +++G TP + L+H++T +++ PD
Sbjct: 430 FGLLGSTEWAEENSRLLQERGVAYINADSSIEGNYTLRVDCTPLMYSLVHNLTKELKSPD 489
Query: 477 --VKGATVHDTWTAE------NGIGNIERLGAVNSDFAAFVQHAGVPSVDVYYGRD---- 536
+G +++++WT + +G+ I +LG+ N DF F Q G+ S Y ++
Sbjct: 490 EGFEGKSLYESWTKKSPSPEFSGMPRISKLGSGN-DFEVFFQRLGIASGRARYTKNWETN 549
Query: 537 ----FPVYHTAFDTYDWMANYGDPLFHRHVTVGSIWGLLALRLSDDLILPFSYISYANQL 596
+P+YH+ ++TY+ + + DP+F H+TV + G + L++ ++LPF YA L
Sbjct: 550 KFSGYPLYHSVYETYELVEKFYDPMFKYHLTVAQVRGGMVFELANSIVLPFDCRDYAVVL 609
Query: 597 QAYKDTL--------NHLLDGSVSLHSLSSSIEELKFAAQEIENEAKRLREQEASSDVAL 656
+ Y D + + SVS SL S+++ A + ++RL++ + S+ + L
Sbjct: 610 RKYADKIYSISMKHPQEMKTYSVSFDSLFSAVKNFTEIASKF---SERLQDFDKSNPIVL 669
Query: 657 FQKRALNDRLMLAERGFLDVDGLRGHPWFKHLVYGPLSNYESALVYFPGIADAVSE-SKE 684
R +ND+LM ER F+D GL P+++H++Y P S+ + A FPGI DA+ + +
Sbjct: 670 ---RMMNDQLMFLERAFIDPLGLPDRPFYRHVIYAPSSHNKYAGESFPGIYDALFDIESK 729
BLAST of Cucsa.242290 vs. Swiss-Prot
Match:
FOLH1_PIG (Glutamate carboxypeptidase 2 OS=Sus scrofa GN=FOLH1 PE=1 SV=1)
HSP 1 Score: 345.1 bits (884), Expect = 1.7e-93
Identity = 222/689 (32.22%), Postives = 367/689 (53.27%), Query Frame = 1
Query: 57 VASYLRSLTLHPHLAGTEPSSETVRYVESHFRDLGLETHSI-QYDALLSYPKSTS---LS 116
+ ++L + T PHLAGTE + + + ++S +++ GL++ + YD LLSYP T +S
Sbjct: 71 IKTFLYNFTRIPHLAGTEQNFQLAKQIQSQWKEFGLDSVELAHYDVLLSYPNKTRPNYIS 130
Query: 117 VLLSNGTVV--------NIPLSENVEGVVQPYHAYSPSGTAYGPAVFVNYGRDEDYRELA 176
++ +G + P ENV VV P+ A+SP G G V+VNY R ED+ +L
Sbjct: 131 IIDEDGNEIFNTSLFEPPPPGYENVSDVVPPFSAFSPQGMPEGDLVYVNYARTEDFFKLE 190
Query: 177 K-MGVTVVGCIAVARKGEFPRGVVVAKAEANGAKGVLLYVEG------------DGFRQ- 236
+ M + G I +AR G+ RG V A+ GAKG++LY + DG+
Sbjct: 191 RDMKINCSGKILIARYGKIFRGNKVKNAQLAGAKGIILYSDPADYFAPGVQSYPDGWNLP 250
Query: 237 --GFERGTVMR--GIGDPLSPGWAAVDGAERLNLNDSEVLKRFPKIPSMPLSAESAEIIL 296
G +RG ++ G GDPL+PG+ A + A RL + ++ L P+IP P+ A+ +L
Sbjct: 251 GGGVQRGNILNLNGAGDPLTPGYPANEYAYRLQIAEAVGL---PRIPVHPIGYSDAQKLL 310
Query: 297 SSLDTASVPPE--WRDK---KANLGSAAVGPGGPIFINFTYQGERKVATIRNVIAVIKGL 356
+ S PP+ W+ N+G G + + KV I NVI ++G
Sbjct: 311 EKMG-GSAPPDDSWKGSLHVPYNVGPGFTGNFSTQKVKMHIHSDNKVKRIYNVIGTLRGA 370
Query: 357 EEPDRFVLMGNHRDAWSFGAVDPNSGTAALLDIARRFSLLRRLGWNPRRTILLCSWDAEE 416
EPDR+V++G HRD+W FG +DP SG A + +I R F L++ GW PRRT+L SWDAEE
Sbjct: 371 VEPDRYVILGGHRDSWVFGGIDPQSGAAVVHEIVRSFGKLKKEGWRPRRTVLFASWDAEE 430
Query: 417 FGMIGSTEWVEQNIVNLGTKAVAYLNVDCAVQGP-GFFAGATPQLDDLLHDVTAQVQDPD 476
+G+ GSTEW E+N L + VAY+N D +++G TP + L++++T ++Q PD
Sbjct: 431 YGLFGSTEWAEENSRILQERGVAYINADSSIEGNYTLRVDCTPLMYSLVYNLTKELQSPD 490
Query: 477 --VKGATVHDTWTAE------NGIGNIERLGAVNSDFAAFVQHAGVPSVDVYYGRD---- 536
+G ++ ++W + +G+ I +LG+ N DF F Q G+ S Y +D
Sbjct: 491 EGFEGKSLFESWNEKSPSPEFSGLPRISKLGSGN-DFEVFFQRLGIASGRARYTKDWVTN 550
Query: 537 ----FPVYHTAFDTYDWMANYGDPLFHRHVTVGSIWGLLALRLSDDLILPFSYISYANQL 596
+P+YH+ ++TY+ + + DP F H+ V + G + L++ ++ PF YA L
Sbjct: 551 KFSSYPLYHSVYETYELVEKFYDPTFKYHLAVAQVRGGIVFELANSVVRPFDCRDYAVVL 610
Query: 597 QAYKD-----TLNH---LLDGSVSLHSLSSSIEELKFAAQEIENEAKRLREQEASSDVAL 656
+ Y D ++NH + SVS SL S+++ A N ++R+++ + ++ + L
Sbjct: 611 RNYADKLYNISMNHPQEMKAYSVSFDSLFSAVKNFTEIA---SNFSERVQDLDKNNPILL 670
Query: 657 FQKRALNDRLMLAERGFLDVDGLRGHPWFKHLVYGPLSNYESALVYFPGIADAVSE-SKE 685
R +ND+LM ER F+ GL +++H++Y P S+ + FPGI DA+ + +
Sbjct: 671 ---RIMNDQLMFLERAFIVPLGLPDRAFYRHVIYAPSSHNKYMGESFPGIYDALFDIENK 730
BLAST of Cucsa.242290 vs. TrEMBL
Match:
A0A0A0LEA0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G790960 PE=4 SV=1)
HSP 1 Score: 1362.8 bits (3526), Expect = 0.0e+00
Identity = 686/701 (97.86%), Postives = 686/701 (97.86%), Query Frame = 1
Query: 1 MVQSPLKQLATICTSRPAPLPTFFFVIIICVLGFYTFHFSTSSSFS-------------L 60
MVQSPLKQLATICTSRPAPLPTFFFVIIICVLGFYTFHFSTSSSFS L
Sbjct: 1 MVQSPLKQLATICTSRPAPLPTFFFVIIICVLGFYTFHFSTSSSFSVTSSPRNSVRFQQL 60
Query: 61 LLSSGSNYTVASYLRSLTLHPHLAGTEPSSETVRYVESHFRDLGLETHSIQYDALLSYPK 120
LLSSGSNYTVASYLRSLTLHPHLAGTEPSSETVRYVESHFRDLGLETHSIQYDALLSYPK
Sbjct: 61 LLSSGSNYTVASYLRSLTLHPHLAGTEPSSETVRYVESHFRDLGLETHSIQYDALLSYPK 120
Query: 121 STSLSVLLSNGTVVNIPLSENVEGVVQPYHAYSPSGTAYGPAVFVNYGRDEDYRELAKMG 180
STSLSVLLSNGTVV IPLSENVEGVVQPYHAYSPSGTAYGPAVFVNYGRDEDYRELAKMG
Sbjct: 121 STSLSVLLSNGTVVYIPLSENVEGVVQPYHAYSPSGTAYGPAVFVNYGRDEDYRELAKMG 180
Query: 181 VTVVGCIAVARKGEFPRGVVVAKAEANGAKGVLLYVEGDGFRQGFERGTVMRGIGDPLSP 240
VTVVGCIAVARKGEFPRGVVVAKAEANGAKGVLLYVEGDGFRQGFERGTVMRGIGDPLSP
Sbjct: 181 VTVVGCIAVARKGEFPRGVVVAKAEANGAKGVLLYVEGDGFRQGFERGTVMRGIGDPLSP 240
Query: 241 GWAAVDGAERLNLNDSEVLKRFPKIPSMPLSAESAEIILSSLDTASVPPEWRDKKANLGS 300
GWAAVDGAERLNLNDSEVLKRFPKIPSMPLSAESAEIILSSLDTASVPPEWRDKKANLGS
Sbjct: 241 GWAAVDGAERLNLNDSEVLKRFPKIPSMPLSAESAEIILSSLDTASVPPEWRDKKANLGS 300
Query: 301 AAVGPGGPIFINFTYQGERKVATIRNVIAVIKGLEEPDRFVLMGNHRDAWSFGAVDPNSG 360
AAVGPGGPIFINFTYQGERKVATIRNVIAVIKGLEEPDRFVLMGNHRDAWSFGAVDPNSG
Sbjct: 301 AAVGPGGPIFINFTYQGERKVATIRNVIAVIKGLEEPDRFVLMGNHRDAWSFGAVDPNSG 360
Query: 361 TAALLDIARRFSLLRRLGWNPRRTILLCSWDAEEFGMIGSTEWVEQNIVNLGTKAVAYLN 420
TAALLDIARRFSLLRRLGWNPRRTILLCSWDAEEFGMIGSTEWVEQNIVNLGTKAVAYLN
Sbjct: 361 TAALLDIARRFSLLRRLGWNPRRTILLCSWDAEEFGMIGSTEWVEQNIVNLGTKAVAYLN 420
Query: 421 VDCAVQGPGFFAGATPQLDDLLHDVTAQVQDPDVKGATVHDTWTAENGIGNIERLGAVNS 480
VDCAVQGPGFFAGATPQLDDLLHDVTAQVQDPDVKGATVHDTWTAENGIGNIERLGAVNS
Sbjct: 421 VDCAVQGPGFFAGATPQLDDLLHDVTAQVQDPDVKGATVHDTWTAENGIGNIERLGAVNS 480
Query: 481 DFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPLFHRHVTVGSIWGLLALRL 540
DFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPLFHRHVTVGSIWGLLALRL
Sbjct: 481 DFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPLFHRHVTVGSIWGLLALRL 540
Query: 541 SDDLILPFSYISYANQLQAYKDTLNHLLDGSVSLHSLSSSIEELKFAAQEIENEAKRLRE 600
SDDLILPFSYISYANQLQAYKDTLNHLLDGSVSLHSLSSSIEELKFAAQEIENEAKRLRE
Sbjct: 541 SDDLILPFSYISYANQLQAYKDTLNHLLDGSVSLHSLSSSIEELKFAAQEIENEAKRLRE 600
Query: 601 QEASSDVALFQKRALNDRLMLAERGFLDVDGLRGHPWFKHLVYGPLSNYESALVYFPGIA 660
QEASSDVALFQKRALNDRLMLAERGFLDVDGLRGHPWFKHLVYGPLSNYESALVYFPGIA
Sbjct: 601 QEASSDVALFQKRALNDRLMLAERGFLDVDGLRGHPWFKHLVYGPLSNYESALVYFPGIA 660
Query: 661 DAVSESKEINKRELEELIQHEIWRVARAIRRAAAALKGELS 689
DAVSESK INKRELEELIQHEIWRVARAIRRAAAALKGELS
Sbjct: 661 DAVSESKVINKRELEELIQHEIWRVARAIRRAAAALKGELS 701
BLAST of Cucsa.242290 vs. TrEMBL
Match:
W9S126_9ROSA (Putative glutamate carboxypeptidase 2 OS=Morus notabilis GN=L484_012423 PE=4 SV=1)
HSP 1 Score: 879.8 bits (2272), Expect = 2.2e-252
Identity = 440/707 (62.23%), Postives = 534/707 (75.53%), Query Frame = 1
Query: 14 TSRPAPLPTFFFVIIICVLGFYTFHFSTSS--------SFSLLLSSGSNYTVASYLRSLT 73
T P PL TF F++I+C+LGFYT H++ S+ + L LS+ +N+T+++YLR LT
Sbjct: 16 TKPPRPLYTFLFIVILCILGFYTLHYAHSAPPLSTSQNALRLFLSAATNFTLSAYLRDLT 75
Query: 74 LHPHLAGTEPSSETVRYVESHFRDLGLETHSIQYDALLSYPKSTSLSVLLSNGTVVNIPL 133
LHPHLAGT+PS T YV +HF LGL H++ YDALLSYP +SLS SNGT + PL
Sbjct: 76 LHPHLAGTKPSLRTTHYVLNHFTSLGLRAHAVAYDALLSYPTHSSLSAHFSNGTELVFPL 135
Query: 134 SENV--------EGVVQPYHAYSPSGTAYGP-AVFVNYGRDEDYRELAKMGVTVVGCIAV 193
SE+ + VV+PYHAYSPSGTA G AVFVN+GR+EDYR L MGV V GC+ +
Sbjct: 136 SESTGSGSGNGNDAVVRPYHAYSPSGTARGAMAVFVNFGREEDYRALGGMGVNVSGCVVI 195
Query: 194 ARKGE-FPRGVVVAKAEANGAKGVLLYVEGDGFRQGFERGTVMRGIGDPLSPGWAAVDGA 253
ARKG PR VV +A NGA VLLY GD FR+GFERGTVMRG+GDPLSPGWA V+G
Sbjct: 196 ARKGGGLPRAAVVERAALNGAVAVLLYTGGDRFRKGFERGTVMRGLGDPLSPGWAGVEGG 255
Query: 254 ERLNLNDSEVLKRFPKIPSMPLSAESAEIILSSLDTASVPPEWRDKKANLGSAAVGPGGP 313
E+L L D EV RFPKIPS+PLS + AE+IL SL A P EWRD +L VGPG P
Sbjct: 256 EKLGLGDDEVSGRFPKIPSLPLSVDDAEVILESLGGAPTPSEWRDA-LDLKVDHVGPG-P 315
Query: 314 IFINFTYQGERKVATIRNVIAVIKGLEEPDRFVLMGNHRDAWSFGAVDPNSGTAALLDIA 373
++F YQGE+KV I NV AVIKGLEEP+R+VL+GNHRDAW++GAVDPNSGTAALLDIA
Sbjct: 316 TLVDFDYQGEKKVVKIHNVFAVIKGLEEPERYVLLGNHRDAWTYGAVDPNSGTAALLDIA 375
Query: 374 RRFSLLRRLGWNPRRTILLCSWDAEEFGM--------------IGSTEWVEQNIVNLGTK 433
RRFSL+ R GW PRRTI+LCSWDAEEFGM IGSTEWVEQN+VNLG+K
Sbjct: 376 RRFSLMMRSGWRPRRTIVLCSWDAEEFGMMSNPVTKSEKKSWEIGSTEWVEQNLVNLGSK 435
Query: 434 AVAYLNVDCAVQGPGFFAGATPQLDDLLHDVTAQVQDPDVKGATVHDTWTAENGIGNIER 493
AVAYLNVDCAVQGPGFFAGATPQLD+LL +V +V+DPD +G T++ W + N NIER
Sbjct: 436 AVAYLNVDCAVQGPGFFAGATPQLDNLLLEVIKKVEDPDSEGETMYQKWASRNSENNIER 495
Query: 494 LGAVNSDFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPLFHRHVTVGSIWG 553
LG V+SDFAAFVQHAGVPSVD+YYG DFP YHTAFD+YDWM NYGDP F RHV V +WG
Sbjct: 496 LGRVDSDFAAFVQHAGVPSVDMYYGADFPTYHTAFDSYDWMINYGDPSFKRHVAVAGVWG 555
Query: 554 LLALRLSDDLILPFSYISYANQLQAYKDTLNHLLDGSVSLHSLSSSIEELKFAAQEIENE 613
LLAL L+DD ILPF+YISYA QL+ +K+ L++LL G VSL LS+SI++ AA+E E E
Sbjct: 556 LLALHLADDPILPFNYISYAEQLKRHKEVLSNLLGGLVSLSPLSTSIQDFASAAKEAEIE 615
Query: 614 AKRLREQEASSDVALFQKRALNDRLMLAERGFLDVDGLRGHPWFKHLVYGPLSNYESALV 673
++LREQE +S++ + +KRALNDRL+ AERGFLD DGL G WFKHL+YGP S+ ES L
Sbjct: 616 TQKLREQETTSELVVLKKRALNDRLIFAERGFLDTDGLEGRRWFKHLIYGPSSDPESKLD 675
Query: 674 YFPGIADAVSESKEINKRELEELIQHEIWRVARAIRRAAAALKGELS 689
+FPGIADA+S + ++ + + +IQHE+WRVARAI+RA +AL+GEL+
Sbjct: 676 FFPGIADAISRTTRMSLSQKKAIIQHEVWRVARAIQRATSALRGELT 720
BLAST of Cucsa.242290 vs. TrEMBL
Match:
V4SW42_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10011190mg PE=4 SV=1)
HSP 1 Score: 871.7 bits (2251), Expect = 5.9e-250
Identity = 438/707 (61.95%), Postives = 537/707 (75.95%), Query Frame = 1
Query: 5 PLKQLATICTSRPAP-LPTFFFVIIICVLGFYTFHFSTSSSFS--------------LLL 64
P +L T +S+P P L TF F++I+C++GFYT H S + L
Sbjct: 4 PFTKLTTTASSKPPPPLITFTFLLILCIIGFYTLHHPYPSPATPPLRNPQTALHFQKTFL 63
Query: 65 SSGSNYTVASYLRSLTLHPHLAGTEPSSETVRYVESHFRDLGLETHSIQYDALLSYPKST 124
S SNYTV+SYLR LT HPHLAGTEPS +TVRYV+SHF L TH+++Y ALLSYP
Sbjct: 64 SLSSNYTVSSYLRDLTHHPHLAGTEPSLDTVRYVQSHFEQLKFNTHTVEYKALLSYPVHA 123
Query: 125 SLSVLLSNGTVVNIPLSEN------VEGVVQPYHAYSPSGTAYGPAVFVNYGRDEDYREL 184
S+S SNGT V + L+E + VVQPYHAYSPSG+AYG VFVNYGR+EDYR L
Sbjct: 124 SVSAHFSNGTTVELSLTEKGISQNAILDVVQPYHAYSPSGSAYGKVVFVNYGREEDYRAL 183
Query: 185 AKMGVTVVGCIAVARKGE-FPRGVVVAKAEANGAKGVLLYVEGDGFRQG-FERGTVMRGI 244
GV V GC+ +ARKG R V+ AEA GA GVLLY E D R G ERGTVMRG+
Sbjct: 184 EAAGVNVSGCVVMARKGSVLSRSGVIFLAEAKGAIGVLLYAEWDRLRGGGVERGTVMRGV 243
Query: 245 GDPLSPGWAAVDGAERLNLNDSEVLKRFPKIPSMPLSAESAEIILSSLDTASVPPEWRDK 304
GDPLSPGWA V+G E L+L DSEV KRFPKIPS+PLS E+A+IIL SL VP ++ +
Sbjct: 244 GDPLSPGWAGVEGGESLDLEDSEVSKRFPKIPSLPLSFENAQIILGSLWGGFVP-QFLND 303
Query: 305 KANLGSAAVGPGGPIFINFTYQGERKVATIRNVIAVIKGLEEPDRFVLMGNHRDAWSFGA 364
+ VGPG P +N T+QG++KVATI NV AVI+GLEEP+R+VL+GNHRDAW++GA
Sbjct: 304 LGRVNGGRVGPG-PTMVNLTFQGKKKVATIYNVFAVIRGLEEPNRYVLLGNHRDAWTYGA 363
Query: 365 VDPNSGTAALLDIARRFSLLRRLGWNPRRTILLCSWDAEEFGMIGSTEWVEQNIVNLGTK 424
+DPNSGTAALLDIARR++LL RLGW+PRRTI+ CSWDAEEFGMIGSTEWVE+N+VNLG K
Sbjct: 364 IDPNSGTAALLDIARRYALLMRLGWSPRRTIIFCSWDAEEFGMIGSTEWVEENLVNLGAK 423
Query: 425 AVAYLNVDCAVQGPGFFAGATPQLDDLLHDVTAQVQDPDVKGATVHDTWTAENGIGNIER 484
AVAYLNVDCAVQGPGFFAGATPQLDD+L +VT V+DP+ + T++D W+A N I NI+R
Sbjct: 424 AVAYLNVDCAVQGPGFFAGATPQLDDILIEVTKMVKDPESESGTLYDQWSAPNRIFNIQR 483
Query: 485 LGAVNSDFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPLFHRHVTVGSIWG 544
LG V+SDFA+FVQHAGVPSVD+YYG+DFPVYHTA DTY W+ + DPLFHRHV V +WG
Sbjct: 484 LGGVDSDFASFVQHAGVPSVDMYYGKDFPVYHTALDTYTWIVGHADPLFHRHVAVAGVWG 543
Query: 545 LLALRLSDDLILPFSYISYANQLQAYKDTLNHLLDGSVSLHSLSSSIEELKFAAQEIENE 604
LLAL L+DD ILPF+++SY++QLQ KD L++LLD +SL L+++I++L AA++ +NE
Sbjct: 544 LLALHLADDSILPFNFLSYSDQLQENKDILSNLLDQRISLDPLTAAIKDLTSAAKDAQNE 603
Query: 605 AKRLREQEASSDVALFQKRALNDRLMLAERGFLDVDGLRGHPWFKHLVYGPLSNYESALV 664
AKRLREQE V + + LNDRLMLAERGFLD DGL+G WFKHL+YGP YES L
Sbjct: 604 AKRLREQEHLGGVEILKIHDLNDRLMLAERGFLDTDGLQGRQWFKHLIYGPHDEYESKLS 663
Query: 665 YFPGIADAVSESKEINKRELEELIQHEIWRVARAIRRAAAALKGELS 689
FPGIADA+S+S ++K++ E LIQHE+WRVARAI+RAA+AL+GEL+
Sbjct: 664 CFPGIADAISQSTSMSKKDGEALIQHEVWRVARAIQRAASALRGELT 708
BLAST of Cucsa.242290 vs. TrEMBL
Match:
L0N3R1_LOTJA (AMP1-like carboxypeptidase OS=Lotus japonicus GN=TCO PE=4 SV=1)
HSP 1 Score: 871.7 bits (2251), Expect = 5.9e-250
Identity = 431/691 (62.37%), Postives = 541/691 (78.29%), Query Frame = 1
Query: 12 ICTSRPAPLPTFFFVIIICVLGFYTFHF-----------STSSSFSLLLSSGSNYTVASY 71
+ +++P+PL T+ ++++C+LGFY +F ++S+ +L LS+ SN T+ASY
Sbjct: 11 VFSTKPSPLSTWVVILVLCILGFYAVYFPRSNAPFPTPLNSSNLQTLFLSTSSNSTIASY 70
Query: 72 LRSLTLHPHLAGTEPSSETVRYVESHFRDLGLETHSIQYDALLSYPKSTSLSVLLSNGTV 131
LR+LTLHPHLAGTEP+++T RYV SHF LGL+TH+ Y ALLSYP S+SL+ S+G+
Sbjct: 71 LRALTLHPHLAGTEPAADTTRYVLSHFTSLGLQTHTATYRALLSYPLSSSLTAHFSDGSS 130
Query: 132 VNIPLSENV--EGVVQPYHAYSPSGTAYGPAVFVNYGRDEDYRELAKMGVTVVGCIAVAR 191
V++PL+E GVVQPYHAYSPSG+ VFVNYGR++DYREL GV V GCI +AR
Sbjct: 131 VDLPLTEPAGGPGVVQPYHAYSPSGSVQARVVFVNYGREKDYREL---GVNVSGCIVIAR 190
Query: 192 KG-EFPRGVVVAKAEANGAKGVLLYVEGDGFRQGFERGTVMRGIGDPLSPGWAAVDGAER 251
KG EF RG VV AE NGA VL+Y +G+ +R GFERG VMRGIGDPLSPGWA V+G E
Sbjct: 191 KGGEFGRGAVVETAEKNGAAAVLIYGDGETWRNGFERGNVMRGIGDPLSPGWAGVEGGES 250
Query: 252 LNLNDSEVLKRFPKIPSMPLSAESAEIILSSLDTASVPPEWRDKKANLGSAAVGPGGPIF 311
L L DSEVLKRFPKIPSMPLSAE A+ ILSSL A VP EWR + S VGPG P
Sbjct: 251 LGLEDSEVLKRFPKIPSMPLSAEVADSILSSLGGAPVPLEWRGALRSKVSH-VGPG-PTV 310
Query: 312 INFTYQGERKVATIRNVIAVIKGLEEPDRFVLMGNHRDAWSFGAVDPNSGTAALLDIARR 371
+NFTYQGE+K+ATI+NV AVIKG EEPDR+VL+GNHRDAW++GAVDP+SGTAALLDIARR
Sbjct: 311 LNFTYQGEKKMATIQNVFAVIKGSEEPDRYVLLGNHRDAWTYGAVDPSSGTAALLDIARR 370
Query: 372 FSLLRRLGWNPRRTILLCSWDAEEFGMIGSTEWVEQNIVNLGTKAVAYLNVDCAVQGPGF 431
+S+L LGW PRRTI+LCSWDAEEFGM+GSTEWVEQN++NLG+K VAYLNVDCAVQGPGF
Sbjct: 371 YSILLGLGWKPRRTIILCSWDAEEFGMVGSTEWVEQNLINLGSKTVAYLNVDCAVQGPGF 430
Query: 432 FAGATPQLDDLLHDVTAQVQDPDVKGATVHDTWTAENGIGNIERLGAVNSDFAAFVQHAG 491
F G+TPQLD L+ +VT +V+DPD + +V+++W A +G NI+RL V+SDFA+FVQHAG
Sbjct: 431 FVGSTPQLDSLILEVTKKVKDPDSEDVSVYESWAAADGGNNIQRLSGVDSDFASFVQHAG 490
Query: 492 VPSVDVYYGRDFPVYHTAFDTYDWMANYGDPLFHRHVTVGSIWGLLALRLSDDLILPFSY 551
+PS+D+YYGRDFPVYHTAFD+Y+WMA +GDP F RHV V +WGLLAL L+DD ILPF+Y
Sbjct: 491 IPSIDIYYGRDFPVYHTAFDSYNWMAEHGDPFFQRHVAVTGVWGLLALHLADDSILPFNY 550
Query: 552 ISYANQLQAYKDTLNHLLDGSVSLHSLSSSIEELKFAAQEIENEAKRLREQEASSDVALF 611
+SYAN+LQ Y + L++LLD SLH L+ SI+E FAA+E ++E K+LR QE +
Sbjct: 551 VSYANELQLYNNRLSNLLDQKTSLHPLAMSIQEFAFAAKEADDELKKLRLQETADCSVDM 610
Query: 612 QKRALNDRLMLAERGFLDVDGLRGHPWFKHLVYGPLSNYESALVYFPGIADAVSESKEIN 671
+KRALNDRLML E+GFLD DGL+G WFKHLV+GP S++E L +FPGI+D+ + S I+
Sbjct: 611 RKRALNDRLMLVEKGFLDADGLQGRQWFKHLVFGPSSDHE-RLNFFPGISDSFTRSTSIS 670
Query: 672 KRELEELIQHEIWRVARAIRRAAAALKGELS 689
+ E IQHE+WRV+RAI RAA+AL+GE +
Sbjct: 671 EGERVAAIQHEVWRVSRAIHRAASALRGEFT 695
BLAST of Cucsa.242290 vs. TrEMBL
Match:
M5VVX9_PRUPE (Uncharacterized protein (Fragment) OS=Prunus persica GN=PRUPE_ppa026745mg PE=4 SV=1)
HSP 1 Score: 869.8 bits (2246), Expect = 2.3e-249
Identity = 429/688 (62.35%), Postives = 536/688 (77.91%), Query Frame = 1
Query: 17 PAPLPTFFFVIIICVLGFYTFHFSTSSSFS-----------LLLSSGSNYTVASYLRSLT 76
P+ TF +++IC+LGFYT H+ + S + LS +N TVASYLR+LT
Sbjct: 1 PSAACTFLSLLVICILGFYTLHYVHPAPSSSNPQTALRFRRIFLSHATNATVASYLRALT 60
Query: 77 LHPHLAGTEPSSETVRYVESHFRDLGLETHSIQYDALLSYPKSTSLSVLLSNGTVVNIPL 136
LH HLAGT PS +T +V +HF LGL+T S Y++LLSYP +SLS SNG+ V+IPL
Sbjct: 61 LHSHLAGTPPSLDTAHFVRAHFMGLGLDTRSAHYNSLLSYPLRSSLSAHFSNGSHVDIPL 120
Query: 137 SEN----VEGVVQPYHAYSPSGTAYGPAVFVNYGRDEDYRELAKMGVTVVGCIA-VARKG 196
+E GVV YHAYSPSG+A+ VF N+G DEDYR LA++GV V GC+ V R G
Sbjct: 121 TEPGLSAENGVVGAYHAYSPSGSAHAKVVFANHGTDEDYRALAELGVNVSGCVVLVKRGG 180
Query: 197 EFPRGVVVAKAEANGAKGVLLYVEGD-GFRQGFERGTVMRGIGDPLSPGWAAVDGAERLN 256
+ PRG V KAE NGA VLLY EGD GF++GFERG V G GDPL+PGWA VDGAERL+
Sbjct: 181 DLPRGEAVGKAEQNGALAVLLYTEGDDGFKKGFERGLVRSGAGDPLTPGWAGVDGAERLD 240
Query: 257 LNDSEVLKRFPKIPSMPLSAESAEIILSSLDTASVPPEWRDKKANLGSAAVGPGGPIFIN 316
D EVLKRFPKIPSMPLSAE+AE++L++L + VP +WR G VGPG P +N
Sbjct: 241 PEDLEVLKRFPKIPSMPLSAEAAELLLATLGGSPVPNQWR-ASLPAGVRGVGPG-PTVMN 300
Query: 317 FTYQGERKVATIRNVIAVIKGLEEPDRFVLMGNHRDAWSFGAVDPNSGTAALLDIARRFS 376
FTYQGE+KV I NV AVI+G EEPDR+VL+GNHRDAW++GAVDPNSGTAALLDIARR++
Sbjct: 301 FTYQGEKKVVAIHNVFAVIRGAEEPDRYVLLGNHRDAWTYGAVDPNSGTAALLDIARRYA 360
Query: 377 LLRRLGWNPRRTILLCSWDAEEFGMIGSTEWVEQNIVNLGTKAVAYLNVDCAVQGPGFFA 436
LL R GW PRRTI++CSWDAEEFGM+GSTEWVEQN+ NLG+KAVAY+NVDCAVQGPG F
Sbjct: 361 LLMRSGWIPRRTIIICSWDAEEFGMVGSTEWVEQNLANLGSKAVAYINVDCAVQGPGLFV 420
Query: 437 GATPQLDDLLHDVTAQVQDPDVKGATVHDTWTAENGIGNIERLGAVNSDFAAFVQHAGVP 496
ATPQLD+L+ +VT +V+DPD +GATV++TW A N +I+RL A++SDFA FVQHAGVP
Sbjct: 421 RATPQLDNLIVEVTKKVKDPDSEGATVYETWMAANRSIDIQRLSALDSDFAPFVQHAGVP 480
Query: 497 SVDVYYGRDFPVYHTAFDTYDWMANYGDPLFHRHVTVGSIWGLLALRLSDDLILPFSYIS 556
S+D+YYGRDFPVYHTAFD YDWM N+GDPLF RHV V IWGLLAL L+DD ILPF+++S
Sbjct: 481 SIDMYYGRDFPVYHTAFDAYDWMVNFGDPLFQRHVAVAGIWGLLALHLADDSILPFNFLS 540
Query: 557 YANQLQAYKDTLNHLLDGSVSLHSLSSSIEELKFAAQEIENEAKRLREQEASSDVALFQK 616
Y +QL+ YKD L+ LLDGSVSLH L+++I+E +AA+E+E+EA+RLR+ +++S++ L +K
Sbjct: 541 YVDQLKHYKDVLSSLLDGSVSLHPLTTAIQEFAYAAKEVEHEAQRLRDVQSTSELVLLKK 600
Query: 617 RALNDRLMLAERGFLDVDGLRGHPWFKHLVYGPLSNYESALVYFPGIADAVSESKEINKR 676
RALNDRLMLAERGFLD DGL G WFKHLV+ P S++ES L++FPG+ADA+ ++K +
Sbjct: 601 RALNDRLMLAERGFLDSDGLEGRHWFKHLVFAPSSDHESELLFFPGVADAIFQAKRTSGM 660
Query: 677 ELEELIQHEIWRVARAIRRAAAALKGEL 688
E + IQHEIWRVARAI+RAA++L+GE+
Sbjct: 661 EGQAKIQHEIWRVARAIQRAASSLRGEI 686
BLAST of Cucsa.242290 vs. TAIR10
Match:
AT3G54720.1 (AT3G54720.1 Peptidase M28 family protein)
HSP 1 Score: 775.4 bits (2001), Expect = 2.9e-224
Identity = 398/697 (57.10%), Postives = 504/697 (72.31%), Query Frame = 1
Query: 16 RPAPLPTFFFVIIICVLGFYTFHFSTSSSFSLL---------------LSSGSNYTVASY 75
+P PL +F FVI++ V FYT H + + LL LSS SN T++SY
Sbjct: 21 QPPPLCSFLFVIVLFVATFYTLHHPDAVTPPLLFSRNAYNALRLRRLFLSSASNATISSY 80
Query: 76 LRSLTLHPHLAGTEPSSETVRYVESHFRDLGLETHSIQYDALLSYPKSTSLSVLLSNGTV 135
LR LT HPHLAGT+PS +T+ YV +HF+ LGLETH +Y+ALLSYP S++ SN T
Sbjct: 81 LRELTRHPHLAGTKPSLDTLHYVFNHFQSLGLETHVAEYEALLSYPTHISVTASFSNTTT 140
Query: 136 VNIPLSENVEG---VVQPYHAYSPSGTAYGPAVFVNYGRDEDYRELAKMGVTVVGCIAVA 195
+ L++ V G VV+PYHAYSPSG+A G VFVN+G + DY L +GV+V GC+ +A
Sbjct: 141 LEFDLND-VPGDSPVVRPYHAYSPSGSAQGNVVFVNHGEERDYHALESIGVSVKGCVVLA 200
Query: 196 RKGE-FPRGVVVAKAEANGAKGVLLYVEGDGFR-QGFERGTVMRGIGDPLSPGWAAVDGA 255
RKGE RG +V AEA GA GVL+Y E DG G ERGTVMRGIGDP+SPGW V G
Sbjct: 201 RKGENLGRGAIVKIAEAKGALGVLIYAENDGGGFGGIERGTVMRGIGDPVSPGWPGVVGG 260
Query: 256 ERLNLNDSEVLKRFPKIPSMPLSAESAEIILSSLDTASVPPEWRDKKANLGSAAVGPG-- 315
E+L+L+D V +RFPKIPS+PLS +AEIIL+SL A P EWR+ S VGPG
Sbjct: 261 EKLSLDDELVTRRFPKIPSLPLSLRNAEIILASLGGARAPLEWRN------SGRVGPGQR 320
Query: 316 ---GPIFINFTYQGERKVATIRNVIAVIKGLEEPDRFVLMGNHRDAWSFGAVDPNSGTAA 375
G + IN T+QGE K+ I NV+ I+G EE DR+V++GNHRDAW++GAVDPNSGT+A
Sbjct: 321 VGPGRMVINMTFQGEMKMKKINNVVVTIRGSEEADRYVILGNHRDAWTYGAVDPNSGTSA 380
Query: 376 LLDIARRFSLLRRLGWNPRRTILLCSWDAEEFGMIGSTEWVEQNIVNLGTKAVAYLNVDC 435
LLDI+RRF+LL + GW PRRTILLCSWDAEEFGMIGSTEW+E+N++NLG AVAYLNVDC
Sbjct: 381 LLDISRRFALLLKSGWRPRRTILLCSWDAEEFGMIGSTEWIEENVLNLGASAVAYLNVDC 440
Query: 436 AVQGPGFFAGATPQLDDLLHDVTAQVQDPDVKGATVHDTWTAENGIGNIERLGAVNSDFA 495
AVQG GFFAGATPQLD LL DV VQDPD G TV +T+ ++N I I+RL V+SDF+
Sbjct: 441 AVQGSGFFAGATPQLDGLLVDVLKLVQDPDAVGLTVEETFKSQNNI--IQRLSRVDSDFS 500
Query: 496 AFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPLFHRHVTVGSIWGLLALRLSDD 555
F+ HAG+PS+D+YYG D+PVYHTAFD+YDWM + DPLFHRHV + IWGLL + L+D+
Sbjct: 501 GFLHHAGIPSIDMYYGADYPVYHTAFDSYDWMIHNADPLFHRHVAMAGIWGLLGILLADE 560
Query: 556 LILPFSYISYANQLQAYKDTLNHLLDGSVSLHSLSSSIEELKFAAQEIENEAKRLREQEA 615
++PF YISYA+QLQA++D L+ LL+G VS++ LS +I+E A+E +EAK+L+ +
Sbjct: 561 PLIPFDYISYADQLQAHRDKLSKLLEGKVSVNPLSMAIQEFSLVAKEAADEAKKLKGKSY 620
Query: 616 SSD--VALFQKRALNDRLMLAERGFLDVDGLRGHPWFKHLVYGPLSNYESALVYFPGIAD 675
S + A ++R LNDRLML ERGFLD +G++G WFKHLVYGP + ES L +FPGIAD
Sbjct: 621 SKNDVAAAAKRRELNDRLMLVERGFLDAEGIKGKEWFKHLVYGPAAEPESKLGFFPGIAD 680
Query: 676 AVSESKEINKRELEELIQHEIWRVARAIRRAAAALKG 686
A++ + E +I+HEIWRVARAI+RA+ ALKG
Sbjct: 681 AIAMNAS------EGIIEHEIWRVARAIQRASKALKG 702
BLAST of Cucsa.242290 vs. TAIR10
Match:
AT5G19740.1 (AT5G19740.1 Peptidase M28 family protein)
HSP 1 Score: 558.1 bits (1437), Expect = 7.4e-159
Identity = 307/694 (44.24%), Postives = 423/694 (60.95%), Query Frame = 1
Query: 25 FVIIICVLGFYTFHFSTSSSF--SLLLSSG--SNYTVASYLRSLTLHPHLAGTEPSSETV 84
FVI F++ S S L +S+ N +VA L +LT PH+AGT ++E
Sbjct: 9 FVIAALSYSFFSLFSSPPKSHYHELFISTSFSDNASVALNLHTLTRRPHVAGTVANAEAA 68
Query: 85 RYVESHFRDLGLETHSIQYDALLSYPKSTSLSVLLSNGTV-VNIPLSENVEG-------V 144
YV S F L++H + Y L+YP SL + ++ + L + G V
Sbjct: 69 EYVRSVFTSSALKSHVVAYQVSLTYPVHRSLVLTPTDSAKPITFLLEQEKLGDNPYANEV 128
Query: 145 VQPYHAYSPSGTAYGPAVFVNYGRDEDYRELAK-MGVTVVGCIAVARKGEFPRGVVVAKA 204
+ +H Y+ SG GP V+ NYGR ED+ L K MGV V G + +AR G+ RG +V A
Sbjct: 129 MPTFHGYAKSGNVSGPVVYANYGRVEDFVRLKKDMGVNVSGAVVIARYGQIYRGDIVKNA 188
Query: 205 EANGAKGVLLYVE-----GDGF--------RQGFERGTVMRGIGDPLSPGWAAVDGAERL 264
GA GV++Y + GD + G + GTV G+GDP +PGWA+VDG ERL
Sbjct: 189 YEAGAVGVVIYTDKRDYGGDEWFPASKWMPPSGVQVGTVYNGLGDPTTPGWASVDGCERL 248
Query: 265 NLNDSEVLKRFPKIPSMPLSAESAEIILSSL----DTASVPPEWRDKKANLGSAAVGPGG 324
+ E+ P IPS+P+SA AE+IL ++ V P VGPG
Sbjct: 249 SDEAVELSGDVPLIPSLPVSAADAEVILKTVVGDVSDGDVYP-------------VGPG- 308
Query: 325 PIFINFTYQGERKVATIRNVIAVIKGLEEPDRFVLMGNHRDAWSFGAVDPNSGTAALLDI 384
P +N +Y GE +A I NVI VI+G EEPDR+V++GNHRDAW+FGAVDPNSGTA L++I
Sbjct: 309 PGVLNLSYIGETVIAKIENVIGVIEGEEEPDRYVILGNHRDAWTFGAVDPNSGTAVLMEI 368
Query: 385 ARRFSLLRRLGWNPRRTILLCSWDAEEFGMIGSTEWVEQNIVNLGTKAVAYLNVDCAVQG 444
A+R L++ GW PRRTI+LC+WDAEE+G+IGSTEWVE+N L ++AVAYLNVDCAV G
Sbjct: 369 AQRLDKLQKRGWKPRRTIILCNWDAEEYGLIGSTEWVEENREMLSSRAVAYLNVDCAVSG 428
Query: 445 PGFFAGATPQLDDLLHDVTAQVQDPDVKGATVHDTWTAENGIGNIERLGAVNSDFAAFVQ 504
PGF A ATPQLD+L+ +V+DPD T++++W + I RLG SD+A+FVQ
Sbjct: 429 PGFHASATPQLDELIKVAAQEVRDPDNATQTIYESWIGSSDSVVIRRLGGGGSDYASFVQ 488
Query: 505 HAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPLFHRHVTVGSIWGLLALRLSDDLILP 564
H GVP VD+ +GR +PVYH+ +D + WM +GDP+F RHV + S+ GL+ALRL+D+ I+P
Sbjct: 489 HVGVPGVDMSFGRGYPVYHSMYDDFTWMEKFGDPMFQRHVAMASVLGLVALRLADEEIIP 548
Query: 565 FSYISYANQL-QAYKDTLNHLLDGSVSLHSLSSSIEELKFAAQEIENEAKRLREQEASSD 624
F+Y SYA +L ++ +D N L ++ + +L SIE+L AA+ I E + ++
Sbjct: 549 FNYTSYALELKKSAEDLENEKLGHNIDVSTLIKSIEDLSTAAKHISLEKEAIK------- 608
Query: 625 VALFQKRALNDRLMLAERGFLDVDGLRGHPWFKHLVYGPLSNYESALVYFPGIADAVSES 684
+ R LNDRLM+AER D DGL PW+KHL+YGP + FPG+ DA+ +
Sbjct: 609 -GALKVRELNDRLMMAERALTDRDGLSERPWYKHLIYGPSKYDDYGSKSFPGVDDAIDNA 668
Query: 685 KEINKRELEELIQHEIWRVARAIRRAAAALKGEL 688
K++N + E +QH+IWRV+RAIR A+ LKGEL
Sbjct: 669 KKLNTKASWENVQHQIWRVSRAIRHASLVLKGEL 680
BLAST of Cucsa.242290 vs. TAIR10
Match:
AT4G07670.1 (AT4G07670.1 protease-associated (PA) domain-containing protein)
HSP 1 Score: 195.3 bits (495), Expect = 1.3e-49
Identity = 113/260 (43.46%), Postives = 153/260 (58.85%), Query Frame = 1
Query: 150 VFVNYGRDEDYRELAK-MGVTVVGCIAVARKGEFPRGVVVAKAEANGAKGVLLYVE---- 209
V+ NYGR ED+ L K MGV V G + +AR G+ + +V A GA GV++Y
Sbjct: 11 VYANYGRVEDFVRLKKDMGVNVSGAVVIARYGQIYKVDIVNNAYEAGAVGVVIYTNKRDY 70
Query: 210 -GDGF--------RQGFERGTVMRGIGDPLSPGWAAVDGAERLNLNDSEVLKRFPKIPSM 269
GD + GF+ GTV G+GDP +PGWA+VDG ERL+ E+ P IPS+
Sbjct: 71 GGDEWFPASKWMPPSGFQVGTVYNGLGDPTTPGWASVDGCERLSDEAVELSGDVPLIPSL 130
Query: 270 PLSAESAEIILSSLDTASVPPEWRDKKANLGSAAVGPGGPIFINFTYQGERKVATIRNVI 329
P+SA AE+IL ++ +G GPG +N +Y V I+NVI
Sbjct: 131 PVSAADAEVILKTI---------------VGDVGPGPG---ILNLSYI----VTKIQNVI 190
Query: 330 AVIKGLEEPDRFVLMGNHRDAWSFGAVDPNSGTAALLD--------IARRFSLLRRLGWN 388
VI+G EEPDR+V++ NHRD W+F AVDPNSGTA L++ IA+R L++ GW
Sbjct: 191 GVIEGEEEPDRYVILRNHRDTWTFRAVDPNSGTAVLMEASKSYLQHIAQRLDKLQKRGWK 248
BLAST of Cucsa.242290 vs. NCBI nr
Match:
gi|778684604|ref|XP_004136724.2| (PREDICTED: probable glutamate carboxypeptidase 2 [Cucumis sativus])
HSP 1 Score: 1362.8 bits (3526), Expect = 0.0e+00
Identity = 686/701 (97.86%), Postives = 686/701 (97.86%), Query Frame = 1
Query: 1 MVQSPLKQLATICTSRPAPLPTFFFVIIICVLGFYTFHFSTSSSFS-------------L 60
MVQSPLKQLATICTSRPAPLPTFFFVIIICVLGFYTFHFSTSSSFS L
Sbjct: 1 MVQSPLKQLATICTSRPAPLPTFFFVIIICVLGFYTFHFSTSSSFSVTSSPRNSVRFQQL 60
Query: 61 LLSSGSNYTVASYLRSLTLHPHLAGTEPSSETVRYVESHFRDLGLETHSIQYDALLSYPK 120
LLSSGSNYTVASYLRSLTLHPHLAGTEPSSETVRYVESHFRDLGLETHSIQYDALLSYPK
Sbjct: 61 LLSSGSNYTVASYLRSLTLHPHLAGTEPSSETVRYVESHFRDLGLETHSIQYDALLSYPK 120
Query: 121 STSLSVLLSNGTVVNIPLSENVEGVVQPYHAYSPSGTAYGPAVFVNYGRDEDYRELAKMG 180
STSLSVLLSNGTVV IPLSENVEGVVQPYHAYSPSGTAYGPAVFVNYGRDEDYRELAKMG
Sbjct: 121 STSLSVLLSNGTVVYIPLSENVEGVVQPYHAYSPSGTAYGPAVFVNYGRDEDYRELAKMG 180
Query: 181 VTVVGCIAVARKGEFPRGVVVAKAEANGAKGVLLYVEGDGFRQGFERGTVMRGIGDPLSP 240
VTVVGCIAVARKGEFPRGVVVAKAEANGAKGVLLYVEGDGFRQGFERGTVMRGIGDPLSP
Sbjct: 181 VTVVGCIAVARKGEFPRGVVVAKAEANGAKGVLLYVEGDGFRQGFERGTVMRGIGDPLSP 240
Query: 241 GWAAVDGAERLNLNDSEVLKRFPKIPSMPLSAESAEIILSSLDTASVPPEWRDKKANLGS 300
GWAAVDGAERLNLNDSEVLKRFPKIPSMPLSAESAEIILSSLDTASVPPEWRDKKANLGS
Sbjct: 241 GWAAVDGAERLNLNDSEVLKRFPKIPSMPLSAESAEIILSSLDTASVPPEWRDKKANLGS 300
Query: 301 AAVGPGGPIFINFTYQGERKVATIRNVIAVIKGLEEPDRFVLMGNHRDAWSFGAVDPNSG 360
AAVGPGGPIFINFTYQGERKVATIRNVIAVIKGLEEPDRFVLMGNHRDAWSFGAVDPNSG
Sbjct: 301 AAVGPGGPIFINFTYQGERKVATIRNVIAVIKGLEEPDRFVLMGNHRDAWSFGAVDPNSG 360
Query: 361 TAALLDIARRFSLLRRLGWNPRRTILLCSWDAEEFGMIGSTEWVEQNIVNLGTKAVAYLN 420
TAALLDIARRFSLLRRLGWNPRRTILLCSWDAEEFGMIGSTEWVEQNIVNLGTKAVAYLN
Sbjct: 361 TAALLDIARRFSLLRRLGWNPRRTILLCSWDAEEFGMIGSTEWVEQNIVNLGTKAVAYLN 420
Query: 421 VDCAVQGPGFFAGATPQLDDLLHDVTAQVQDPDVKGATVHDTWTAENGIGNIERLGAVNS 480
VDCAVQGPGFFAGATPQLDDLLHDVTAQVQDPDVKGATVHDTWTAENGIGNIERLGAVNS
Sbjct: 421 VDCAVQGPGFFAGATPQLDDLLHDVTAQVQDPDVKGATVHDTWTAENGIGNIERLGAVNS 480
Query: 481 DFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPLFHRHVTVGSIWGLLALRL 540
DFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPLFHRHVTVGSIWGLLALRL
Sbjct: 481 DFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPLFHRHVTVGSIWGLLALRL 540
Query: 541 SDDLILPFSYISYANQLQAYKDTLNHLLDGSVSLHSLSSSIEELKFAAQEIENEAKRLRE 600
SDDLILPFSYISYANQLQAYKDTLNHLLDGSVSLHSLSSSIEELKFAAQEIENEAKRLRE
Sbjct: 541 SDDLILPFSYISYANQLQAYKDTLNHLLDGSVSLHSLSSSIEELKFAAQEIENEAKRLRE 600
Query: 601 QEASSDVALFQKRALNDRLMLAERGFLDVDGLRGHPWFKHLVYGPLSNYESALVYFPGIA 660
QEASSDVALFQKRALNDRLMLAERGFLDVDGLRGHPWFKHLVYGPLSNYESALVYFPGIA
Sbjct: 601 QEASSDVALFQKRALNDRLMLAERGFLDVDGLRGHPWFKHLVYGPLSNYESALVYFPGIA 660
Query: 661 DAVSESKEINKRELEELIQHEIWRVARAIRRAAAALKGELS 689
DAVSESK INKRELEELIQHEIWRVARAIRRAAAALKGELS
Sbjct: 661 DAVSESKVINKRELEELIQHEIWRVARAIRRAAAALKGELS 701
BLAST of Cucsa.242290 vs. NCBI nr
Match:
gi|659084628|ref|XP_008442988.1| (PREDICTED: probable glutamate carboxypeptidase 2 isoform X1 [Cucumis melo])
HSP 1 Score: 1313.9 bits (3399), Expect = 0.0e+00
Identity = 659/701 (94.01%), Postives = 672/701 (95.86%), Query Frame = 1
Query: 1 MVQSPLKQLATICTSRPAPLPTFFFVIIICVLGFYTFHFSTSSSFS-------------L 60
MVQ PLKQLATICTSRPAPLPTFFFVIIICVLGFY FHFS+SSSFS L
Sbjct: 1 MVQPPLKQLATICTSRPAPLPTFFFVIIICVLGFYAFHFSSSSSFSVTSSPRNSVRFQQL 60
Query: 61 LLSSGSNYTVASYLRSLTLHPHLAGTEPSSETVRYVESHFRDLGLETHSIQYDALLSYPK 120
LLSSGSNYTVASYLRSLTLHPHLAGTEPSSETVRYVESHFRDLGLETHSIQYDALLSYPK
Sbjct: 61 LLSSGSNYTVASYLRSLTLHPHLAGTEPSSETVRYVESHFRDLGLETHSIQYDALLSYPK 120
Query: 121 STSLSVLLSNGTVVNIPLSENVEGVVQPYHAYSPSGTAYGPAVFVNYGRDEDYRELAKMG 180
STSLSVL SNG+VVNIPLSENVEGVVQPYHAYSPSGT YGPAVFVNYGRDEDYRELAKMG
Sbjct: 121 STSLSVLFSNGSVVNIPLSENVEGVVQPYHAYSPSGTVYGPAVFVNYGRDEDYRELAKMG 180
Query: 181 VTVVGCIAVARKGEFPRGVVVAKAEANGAKGVLLYVEGDGFRQGFERGTVMRGIGDPLSP 240
VTVVGCIAVARKGEFPRGVVVAKAEANGAKGVLLY E D FRQGFERGTVMRGIGDPLSP
Sbjct: 181 VTVVGCIAVARKGEFPRGVVVAKAEANGAKGVLLYTEDDRFRQGFERGTVMRGIGDPLSP 240
Query: 241 GWAAVDGAERLNLNDSEVLKRFPKIPSMPLSAESAEIILSSLDTASVPPEWRDKKANLGS 300
GWAAVDGAERLNLNDSEVLKRFPKIPSMPLS+ESAEIIL+SLDTASVPPEWRDK+ANL S
Sbjct: 241 GWAAVDGAERLNLNDSEVLKRFPKIPSMPLSSESAEIILNSLDTASVPPEWRDKEANLRS 300
Query: 301 AAVGPGGPIFINFTYQGERKVATIRNVIAVIKGLEEPDRFVLMGNHRDAWSFGAVDPNSG 360
AAVGPGGP+FINFTYQGERKVATIRNVIAVIKGLEEPDRFVLMGNHRDAWSFGAVDPNSG
Sbjct: 301 AAVGPGGPMFINFTYQGERKVATIRNVIAVIKGLEEPDRFVLMGNHRDAWSFGAVDPNSG 360
Query: 361 TAALLDIARRFSLLRRLGWNPRRTILLCSWDAEEFGMIGSTEWVEQNIVNLGTKAVAYLN 420
TAALLDIARRF+LLR+LGWNPRRTILLCSWDAEEFGMIGSTEWVEQNIVNLGTKAVAYLN
Sbjct: 361 TAALLDIARRFALLRKLGWNPRRTILLCSWDAEEFGMIGSTEWVEQNIVNLGTKAVAYLN 420
Query: 421 VDCAVQGPGFFAGATPQLDDLLHDVTAQVQDPDVKGATVHDTWTAENGIGNIERLGAVNS 480
VDCAVQGPGFFAGATPQLDDLLHDVTAQVQDPDVKGATVHDTWTAENGIGNIERLGAVNS
Sbjct: 421 VDCAVQGPGFFAGATPQLDDLLHDVTAQVQDPDVKGATVHDTWTAENGIGNIERLGAVNS 480
Query: 481 DFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPLFHRHVTVGSIWGLLALRL 540
DFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPLFHRHVTVGSIWGLLALRL
Sbjct: 481 DFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPLFHRHVTVGSIWGLLALRL 540
Query: 541 SDDLILPFSYISYANQLQAYKDTLNHLLDGSVSLHSLSSSIEELKFAAQEIENEAKRLRE 600
SDDLILPFSY+SYANQLQAYKDTLNHLLDGSVSL +LS+SIEELK AAQEIENEAKRLRE
Sbjct: 541 SDDLILPFSYVSYANQLQAYKDTLNHLLDGSVSLRTLSTSIEELKSAAQEIENEAKRLRE 600
Query: 601 QEASSDVALFQKRALNDRLMLAERGFLDVDGLRGHPWFKHLVYGPLSNYESALVYFPGIA 660
QE SSDVALFQKRALNDRLMLAERGFLDVDGLRG PWFKHLVYGP S+YESALVYFPGIA
Sbjct: 601 QETSSDVALFQKRALNDRLMLAERGFLDVDGLRGRPWFKHLVYGPPSDYESALVYFPGIA 660
Query: 661 DAVSESKEINKRELEELIQHEIWRVARAIRRAAAALKGELS 689
DAVSESKE+NK LEELIQHEIWRVARAI RAAAALKGELS
Sbjct: 661 DAVSESKEMNKMGLEELIQHEIWRVARAIGRAAAALKGELS 701
BLAST of Cucsa.242290 vs. NCBI nr
Match:
gi|659084630|ref|XP_008442989.1| (PREDICTED: probable glutamate carboxypeptidase 2 isoform X2 [Cucumis melo])
HSP 1 Score: 1064.7 bits (2752), Expect = 6.9e-308
Identity = 528/558 (94.62%), Postives = 537/558 (96.24%), Query Frame = 1
Query: 1 MVQSPLKQLATICTSRPAPLPTFFFVIIICVLGFYTFHFSTSSSFS-------------L 60
MVQ PLKQLATICTSRPAPLPTFFFVIIICVLGFY FHFS+SSSFS L
Sbjct: 1 MVQPPLKQLATICTSRPAPLPTFFFVIIICVLGFYAFHFSSSSSFSVTSSPRNSVRFQQL 60
Query: 61 LLSSGSNYTVASYLRSLTLHPHLAGTEPSSETVRYVESHFRDLGLETHSIQYDALLSYPK 120
LLSSGSNYTVASYLRSLTLHPHLAGTEPSSETVRYVESHFRDLGLETHSIQYDALLSYPK
Sbjct: 61 LLSSGSNYTVASYLRSLTLHPHLAGTEPSSETVRYVESHFRDLGLETHSIQYDALLSYPK 120
Query: 121 STSLSVLLSNGTVVNIPLSENVEGVVQPYHAYSPSGTAYGPAVFVNYGRDEDYRELAKMG 180
STSLSVL SNG+VVNIPLSENVEGVVQPYHAYSPSGT YGPAVFVNYGRDEDYRELAKMG
Sbjct: 121 STSLSVLFSNGSVVNIPLSENVEGVVQPYHAYSPSGTVYGPAVFVNYGRDEDYRELAKMG 180
Query: 181 VTVVGCIAVARKGEFPRGVVVAKAEANGAKGVLLYVEGDGFRQGFERGTVMRGIGDPLSP 240
VTVVGCIAVARKGEFPRGVVVAKAEANGAKGVLLY E D FRQGFERGTVMRGIGDPLSP
Sbjct: 181 VTVVGCIAVARKGEFPRGVVVAKAEANGAKGVLLYTEDDRFRQGFERGTVMRGIGDPLSP 240
Query: 241 GWAAVDGAERLNLNDSEVLKRFPKIPSMPLSAESAEIILSSLDTASVPPEWRDKKANLGS 300
GWAAVDGAERLNLNDSEVLKRFPKIPSMPLS+ESAEIIL+SLDTASVPPEWRDK+ANL S
Sbjct: 241 GWAAVDGAERLNLNDSEVLKRFPKIPSMPLSSESAEIILNSLDTASVPPEWRDKEANLRS 300
Query: 301 AAVGPGGPIFINFTYQGERKVATIRNVIAVIKGLEEPDRFVLMGNHRDAWSFGAVDPNSG 360
AAVGPGGP+FINFTYQGERKVATIRNVIAVIKGLEEPDRFVLMGNHRDAWSFGAVDPNSG
Sbjct: 301 AAVGPGGPMFINFTYQGERKVATIRNVIAVIKGLEEPDRFVLMGNHRDAWSFGAVDPNSG 360
Query: 361 TAALLDIARRFSLLRRLGWNPRRTILLCSWDAEEFGMIGSTEWVEQNIVNLGTKAVAYLN 420
TAALLDIARRF+LLR+LGWNPRRTILLCSWDAEEFGMIGSTEWVEQNIVNLGTKAVAYLN
Sbjct: 361 TAALLDIARRFALLRKLGWNPRRTILLCSWDAEEFGMIGSTEWVEQNIVNLGTKAVAYLN 420
Query: 421 VDCAVQGPGFFAGATPQLDDLLHDVTAQVQDPDVKGATVHDTWTAENGIGNIERLGAVNS 480
VDCAVQGPGFFAGATPQLDDLLHDVTAQVQDPDVKGATVHDTWTAENGIGNIERLGAVNS
Sbjct: 421 VDCAVQGPGFFAGATPQLDDLLHDVTAQVQDPDVKGATVHDTWTAENGIGNIERLGAVNS 480
Query: 481 DFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPLFHRHVTVGSIWGLLALRL 540
DFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPLFHRHVTVGSIWGLLALRL
Sbjct: 481 DFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPLFHRHVTVGSIWGLLALRL 540
Query: 541 SDDLILPFSYISYANQLQ 546
SDDLILPFSY+SYANQLQ
Sbjct: 541 SDDLILPFSYVSYANQLQ 558
BLAST of Cucsa.242290 vs. NCBI nr
Match:
gi|1009173641|ref|XP_015867919.1| (PREDICTED: probable glutamate carboxypeptidase 2 [Ziziphus jujuba])
HSP 1 Score: 919.8 bits (2376), Expect = 2.7e-264
Identity = 451/687 (65.65%), Postives = 543/687 (79.04%), Query Frame = 1
Query: 22 TFFFVIIICVLGFYTFHFSTSSSFS-----------LLLSSGSNYTVASYLRSLTLHPHL 81
+F FV+++C+LGFY H+ S+ S LS+ +N T+ASYLR+LTLHPHL
Sbjct: 22 SFLFVVLLCILGFYALHYPQSAPSSSTLRNALRFRQTFLSAATNRTIASYLRALTLHPHL 81
Query: 82 AGTEPSSETVRYVESHFRDLGLETHSIQYDALLSYPKSTSLSVLLSNGTVVNIPLSE--- 141
AGT+PS ET YVE+HFRD GLETH ++Y LLSYP SLS SNG+ V +PL E
Sbjct: 82 AGTKPSLETTSYVETHFRDYGLETHRVEYKTLLSYPAHASLSAHFSNGSYVELPLMELGL 141
Query: 142 ----NVEGVVQPYHAYSPSGTAYGPAVFVNYGRDEDYRELAKMGVTVVGCIAVARKGEFP 201
+G+VQPYHAYSPSG+A+ AVFVNYGRDEDY LA +GV V GC+ +ARKG
Sbjct: 142 PDHQASQGLVQPYHAYSPSGSAHAKAVFVNYGRDEDYLALAALGVNVSGCVVIARKGGLS 201
Query: 202 RGVVVAKAEANGAKGVLLYVEGDGFRQGFERGTVMRGIGDPLSPGWAAVDGAERLNLNDS 261
RG VV KAE NGA VLLY EG+ FR+GFERGTVM+G+GDPLSPGWA VDGAE L L DS
Sbjct: 202 RGAVVEKAEVNGALAVLLYAEGERFRKGFERGTVMKGLGDPLSPGWAGVDGAESLELEDS 261
Query: 262 EVLKRFPKIPSMPLSAESAEIILSSLDTASVPPEWRDKKANLGSAAVGPG-GPIFINFTY 321
EVLKRFPKIPSMPLS E+AEIIL SL A VP EWRD + L S G G GP +NFTY
Sbjct: 262 EVLKRFPKIPSMPLSFEAAEIILGSLAGAPVPNEWRDTEPLLQSKVGGVGPGPTTVNFTY 321
Query: 322 QGERKVATIRNVIAVIKGLEEPDRFVLMGNHRDAWSFGAVDPNSGTAALLDIARRFSLLR 381
QGE K+ATI NV +I+GLEEPDR+VL+GNHRDAW++GAVDPNSGTAALLDIARRFSL+
Sbjct: 322 QGENKMATIHNVFGLIRGLEEPDRYVLLGNHRDAWTYGAVDPNSGTAALLDIARRFSLMM 381
Query: 382 RLGWNPRRTILLCSWDAEEFGMIGSTEWVEQNIVNLGTKAVAYLNVDCAVQGPGFFAGAT 441
R GWNPRRTI+LCSWDAEEFGMIGSTEWVEQN+VNLG+KAVAYLNVDCAVQGPGFFAGAT
Sbjct: 382 RSGWNPRRTIILCSWDAEEFGMIGSTEWVEQNLVNLGSKAVAYLNVDCAVQGPGFFAGAT 441
Query: 442 PQLDDLLHDVTAQVQDPDVKGATVHDTWTAENGIGNIERLGAVNSDFAAFVQHAGVPSVD 501
PQLD LL +VT +V+DPD + T++ WTA N +I+RLG V+SDFA FVQHAGVPSVD
Sbjct: 442 PQLDSLLLEVTKKVKDPDSEAVTIYQQWTATNTDISIQRLGGVDSDFAPFVQHAGVPSVD 501
Query: 502 VYYGRDFPVYHTAFDTYDWMANYGDPLFHRHVTVGSIWGLLALRLSDDLILPFSYISYAN 561
+YYGRDFPVYHTAFD+Y+WM NYGDPLF RHV V +WGLL L L+DD ILPFSY+SYA+
Sbjct: 502 IYYGRDFPVYHTAFDSYEWMTNYGDPLFQRHVAVAGVWGLLGLHLADDSILPFSYLSYAD 561
Query: 562 QL-QAYKDTLNHLLDGSVSLHSLSSSIEELKFAAQEIENEAKRLREQEASSDVALFQKRA 621
QL + Y++ ++LLD +V+L+ L +SI+E AA+E E EAK+LREQE +SD+ +KRA
Sbjct: 562 QLKEGYRNVWSNLLDTTVTLNPLMTSIQEFVTAAKEAEIEAKKLREQETTSDLVTLKKRA 621
Query: 622 LNDRLMLAERGFLDVDGLRGHPWFKHLVYGPLSNYESALVYFPGIADAVSESKEINKREL 681
LNDRLMLAERGFLD DGL+G WFKHL+YGP +YES L FPGI+DA+S ++ +++R+
Sbjct: 622 LNDRLMLAERGFLDADGLQGRQWFKHLIYGPPIDYESKLQMFPGISDAISRARNMSERQG 681
Query: 682 EELIQHEIWRVARAIRRAAAALKGELS 689
+ +I+HEIWRVARAI+RAA+ L+G+ +
Sbjct: 682 QAIIEHEIWRVARAIQRAASVLRGQFT 708
BLAST of Cucsa.242290 vs. NCBI nr
Match:
gi|1009161401|ref|XP_015898877.1| (PREDICTED: probable glutamate carboxypeptidase 2 [Ziziphus jujuba])
HSP 1 Score: 918.7 bits (2373), Expect = 6.1e-264
Identity = 456/708 (64.41%), Postives = 549/708 (77.54%), Query Frame = 1
Query: 1 MVQSPLKQLATICTSRPAPLPTFFFVIIICVLGFYTFHFSTSSSFS-----------LLL 60
M Q K + T + +F FV+++C+LGFY H+ S+ S L
Sbjct: 1 MAQLCSKPTTAMFTGKTHHSCSFLFVVLLCILGFYALHYPQSAPSSSALRNALRFRQTFL 60
Query: 61 SSGSNYTVASYLRSLTLHPHLAGTEPSSETVRYVESHFRDLGLETHSIQYDALLSYPKST 120
S+ +N T+ASYLR+LTLHPHLAGT+PS ET YVE+HFRD GLETH ++Y LLSYP
Sbjct: 61 SAATNRTIASYLRALTLHPHLAGTKPSLETTSYVETHFRDYGLETHRVEYKTLLSYPAHA 120
Query: 121 SLSVLLSNGTVVNIPLSE-------NVEGVVQPYHAYSPSGTAYGPAVFVNYGRDEDYRE 180
SLS SNG+ V +PL E +G+VQPYHAYSPSG+A+ AVFVNYGRDEDY
Sbjct: 121 SLSAHFSNGSYVELPLMELGLPDHQASQGLVQPYHAYSPSGSAHAKAVFVNYGRDEDYLA 180
Query: 181 LAKMGVTVVGCIAVARKGEFPRGVVVAKAEANGAKGVLLYVEGDGFRQGFERGTVMRGIG 240
LA +GV V GC+ +ARKG RG VV KAEANGA VLLY EG+ FR+GFERGTVM+G+G
Sbjct: 181 LAALGVNVSGCVVIARKGGLSRGAVVEKAEANGALAVLLYAEGERFRKGFERGTVMKGLG 240
Query: 241 DPLSPGWAAVDGAERLNLNDSEVLKRFPKIPSMPLSAESAEIILSSLDTASVPPEWRDKK 300
DPLSPGWA VDGAE L L DSEVLKRFPKIPSMPLS E+AEIIL SL A VP EWRD
Sbjct: 241 DPLSPGWAGVDGAESLELEDSEVLKRFPKIPSMPLSFEAAEIILGSLAGAPVPNEWRDTA 300
Query: 301 ANLGSAAVGPG-GPIFINFTYQGERKVATIRNVIAVIKGLEEPDRFVLMGNHRDAWSFGA 360
L S G G GP +NFTYQGE K+ATI NV +I+GLEEPDR+VL+GNHRDAW++GA
Sbjct: 301 PLLQSKVGGVGPGPTTVNFTYQGENKMATIHNVFGLIRGLEEPDRYVLLGNHRDAWTYGA 360
Query: 361 VDPNSGTAALLDIARRFSLLRRLGWNPRRTILLCSWDAEEFGMIGSTEWVEQNIVNLGTK 420
VDPNSGTAALLDIARRFSL+ R GWNPRRTI+LCSWDAEEFGMIGSTEWVEQN+VNLG+K
Sbjct: 361 VDPNSGTAALLDIARRFSLMMRSGWNPRRTIILCSWDAEEFGMIGSTEWVEQNLVNLGSK 420
Query: 421 AVAYLNVDCAVQGPGFFAGATPQLDDLLHDVTAQVQDPDVKGATVHDTWTAENGIGNIER 480
AVAYLNVDCAVQGPGFFAGATPQLD LL +VT +V+DPD + T++ WTA N +I+R
Sbjct: 421 AVAYLNVDCAVQGPGFFAGATPQLDSLLLEVTKKVKDPDSEAVTIYQQWTATNTDISIQR 480
Query: 481 LGAVNSDFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPLFHRHVTVGSIWG 540
LG V+SDFA FVQHAGVPSVD+YYGRDFPVYHTAFD+Y+WM NYGDPLF RHV V +WG
Sbjct: 481 LGGVDSDFAPFVQHAGVPSVDIYYGRDFPVYHTAFDSYEWMTNYGDPLFQRHVAVAGVWG 540
Query: 541 LLALRLSDDLILPFSYISYANQL-QAYKDTLNHLLDGSVSLHSLSSSIEELKFAAQEIEN 600
LL L L+DD ILPFSY+SYA+QL + Y++ ++LLD V+L+ L +SI+E AA+E E
Sbjct: 541 LLGLHLADDSILPFSYLSYADQLKEGYRNVWSNLLDTPVTLNPLMTSIQEFVTAAKEAEI 600
Query: 601 EAKRLREQEASSDVALFQKRALNDRLMLAERGFLDVDGLRGHPWFKHLVYGPLSNYESAL 660
EAK+LREQE +SD+ +KRALNDRLMLAERGFLD DGL+G WFKHL+YGP+ +YES L
Sbjct: 601 EAKKLREQETTSDLVTLKKRALNDRLMLAERGFLDADGLQGRQWFKHLIYGPI-DYESKL 660
Query: 661 VYFPGIADAVSESKEINKRELEELIQHEIWRVARAIRRAAAALKGELS 689
FPGI+DA+S ++ +++R+ + +I+HEIWRVARAI+RAA+ L+G+ +
Sbjct: 661 QMFPGISDAISRARNMSERQGQAIIEHEIWRVARAIQRAASVLRGQFT 707
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| GCP2_ARATH | 5.2e-223 | 57.10 | Probable glutamate carboxypeptidase 2 OS=Arabidopsis thaliana GN=AMP1 PE=1 SV=3 | [more] |
| FOLH1_RAT | 1.1e-98 | 33.86 | Glutamate carboxypeptidase 2 OS=Rattus norvegicus GN=Folh1 PE=1 SV=1 | [more] |
| FOLH1_MOUSE | 2.3e-98 | 34.01 | Glutamate carboxypeptidase 2 OS=Mus musculus GN=Folh1 PE=1 SV=2 | [more] |
| FOLH1_HUMAN | 6.8e-98 | 32.99 | Glutamate carboxypeptidase 2 OS=Homo sapiens GN=FOLH1 PE=1 SV=1 | [more] |
| FOLH1_PIG | 1.7e-93 | 32.22 | Glutamate carboxypeptidase 2 OS=Sus scrofa GN=FOLH1 PE=1 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A0A0LEA0_CUCSA | 0.0e+00 | 97.86 | Uncharacterized protein OS=Cucumis sativus GN=Csa_3G790960 PE=4 SV=1 | [more] |
| W9S126_9ROSA | 2.2e-252 | 62.23 | Putative glutamate carboxypeptidase 2 OS=Morus notabilis GN=L484_012423 PE=4 SV=... | [more] |
| V4SW42_9ROSI | 5.9e-250 | 61.95 | Uncharacterized protein OS=Citrus clementina GN=CICLE_v10011190mg PE=4 SV=1 | [more] |
| L0N3R1_LOTJA | 5.9e-250 | 62.37 | AMP1-like carboxypeptidase OS=Lotus japonicus GN=TCO PE=4 SV=1 | [more] |
| M5VVX9_PRUPE | 2.3e-249 | 62.35 | Uncharacterized protein (Fragment) OS=Prunus persica GN=PRUPE_ppa026745mg PE=4 S... | [more] |
