Cucsa.237230 (gene) Cucumber (Gy14) v1

NameCucsa.237230
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionPentatricopeptide repeat-containing family protein
Locationscaffold02023 : 207897 .. 209963 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAATTTCTTCATCCTTCATTATCTCCCTTCATCTTCAACCTTTCACTCCCAATTCTCTCGCCCCGGCCACCGCTATTTGTAATTCAGGCCACCGACTTTCCCGAATCAAATCCACCACAGATACACCGCCGTCAAAAATCAAAATAGTTTCCAAATTTCGGAACCGAAAACGCCCAACTTTTGCCGAGAAAGATGCTTTTCCTTCATCTTTACCACTTCACACCAAGAACCCTCATGCCATTTACGAGGACGTTCAAAGATTTGCACGGCAAAATAAGCTCAAAGAGGCACTTACGATTATGGACTATGTGGATCAACAAGGCATTCCAGTTAATGCCACTACATTTTCTTCTCTTATTACGGCTTGTGTTAGAACAAAATCGATGACTTATGCGAAACAGATTCACGCGCATATTCGGATAAATGGGCTTGAAAACAATGAATTTATACGTACAAGGCTTGTGCATATGTATACTGCTTGTGGGTCCTTGGAAGAGGCACAGAAGCTGTTTGATGAAAGTTCTAGCAAGAGTGTTTATCCTTGGAATGCGTTGCTTAGAGGCACTGTAATGGCGGGTCGGAGGGATTATCGCAGCATTCTATCGACTTATGCAGAAATGCGAAGATTGGGAGTTGAATTGAACGTTTACTCCTTTGCTAATATCATTAAAAGCTTTGCAGGTGCATCGGCGTTTACTCAGGGGCTCAAGGCTCATGGCCTTTTGATTAAAAATGGATTGATTGGCAGTTCACTTCTTGGGACAACTTTGGTTGATATGTACTTCAAATGTGGTAAGATCAAACTTGCCCGCCAGATGTTTGGGGAAATTACCGAGAGAGATGTTGTGGTTTGGGGATCAATAATTGCTGGGTTTGCTCACAATCGACTCCAAAGGGAAGCTTTGGAATATACAAGGAGGATGATAGACGATGGAATTAGACCAAATTCGGTTATATTGACGACCATTCTTCCAGTTATTGGAGAAATCTGGGCGAGGAGATTAGGTCAGGAAGTTCATGCTTATGTTATAAAGACGAAAAGTTACTCGAAGCAGATATTTATTCAGTCTGCTTTGATTGATATGTACTGCAAGTGTGGGGACATCGGTTCAGGTAGAGCCGTTTTTTATGCATCCATGGAGAGGAATGCTATATGCTGGACTGCTTTGATGTCTGGTTATGCTTTAAATGGTAGGCTAGAACAGGCTGTAAGATCAGTTATTTGGATGCAGCAGGAAGGATTTAGACCGGATATCGTAACTGTTGCAACGATTCTTCCAGTTTGTGCTCAATTGAGAGCTCTGAGACCCGGAAAAGAGATTCATGCTTACGCTATGAAGAATTGCTTCCTACCGAATGTATCTATCGTTTCATCTTTGATGGTTATGTACTCAAAATGTGGAGTAATGGACTATACTCTAAAGCTTTTCAACGGCATGGAGCAAAGAAATGTGATCTTATGGACAGCAATGATTGATTCGTACATAGAGAATCAATGTCCACACGAAGCAATTGATATATTCAGAGCGATGCAGCTTTCAAAGCATCGACCAGATACCGTTACCATGTCTAGAATTCTCTATATATGCAGTGAACAAAAAATGCTGAAGATGGGTAAGGAGATACATGGACAAGTCTTGAAGAGGAAATTTGAGCCGGTCCATTTCGTTTCTGCCGAGCTAGTGAAACTATATGGGAAGTGTGGAGCAGTAAAAATGGCAAAAATGGTTTTTGAGGCAATCCCTGTGAAAGGGCCAATGACATGGACTGCCATCATTGAAGCTTACGGAGAAAGTGGAGAGTTTCAGGAAGCAATCGATCTGTTTGACCGAATGAGATCACGTGGTATCTCTCCAAACCATTTCACTTTCAAAGTGGTTCTATCTATTTGCAAGGAAGCTGGTTTTGTTGATGAAGCACTGCGCATCTTTAAGCTAATGTCTGTTAGGTATAAGATTAAGCCATCTGAAGAACATTACTCTTTAGTCATTGCGATTCTTACTCGGTTTGGTCGACTCGAGGAGGCTAGAAGGTATGTACAAATGCTTTCTTCATTGTCATAA

mRNA sequence

ATGGAAATTTCTTCATCCTTCATTATCTCCCTTCATCTTCAACCTTTCACTCCCAATTCTCTCGCCCCGGCCACCGCTATTTGTAATTCAGGCCACCGACTTTCCCGAATCAAATCCACCACAGATACACCGCCGTCAAAAATCAAAATAGTTTCCAAATTTCGGAACCGAAAACGCCCAACTTTTGCCGAGAAAGATGCTTTTCCTTCATCTTTACCACTTCACACCAAGAACCCTCATGCCATTTACGAGGACGTTCAAAGATTTGCACGGCAAAATAAGCTCAAAGAGGCACTTACGATTATGGACTATGTGGATCAACAAGGCATTCCAGTTAATGCCACTACATTTTCTTCTCTTATTACGGCTTGTGTTAGAACAAAATCGATGACTTATGCGAAACAGATTCACGCGCATATTCGGATAAATGGGCTTGAAAACAATGAATTTATACGTACAAGGCTTGTGCATATGTATACTGCTTGTGGGTCCTTGGAAGAGGCACAGAAGCTGTTTGATGAAAGTTCTAGCAAGAGTGTTTATCCTTGGAATGCGTTGCTTAGAGGCACTGTAATGGCGGGTCGGAGGGATTATCGCAGCATTCTATCGACTTATGCAGAAATGCGAAGATTGGGAGTTGAATTGAACGTTTACTCCTTTGCTAATATCATTAAAAGCTTTGCAGGTGCATCGGCGTTTACTCAGGGGCTCAAGGCTCATGGCCTTTTGATTAAAAATGGATTGATTGGCAGTTCACTTCTTGGGACAACTTTGGTTGATATGTACTTCAAATGTGGTAAGATCAAACTTGCCCGCCAGATGTTTGGGGAAATTACCGAGAGAGATGTTGTGGTTTGGGGATCAATAATTGCTGGGTTTGCTCACAATCGACTCCAAAGGGAAGCTTTGGAATATACAAGGAGGATGATAGACGATGGAATTAGACCAAATTCGGTTATATTGACGACCATTCTTCCAGTTATTGGAGAAATCTGGGCGAGGAGATTAGGTCAGGAAGTTCATGCTTATGTTATAAAGACGAAAAGTTACTCGAAGCAGATATTTATTCAGTCTGCTTTGATTGATATGTACTGCAAGTGTGGGGACATCGGTTCAGGTAGAGCCGTTTTTTATGCATCCATGGAGAGGAATGCTATATGCTGGACTGCTTTGATGTCTGGTTATGCTTTAAATGGTAGGCTAGAACAGGCTGTAAGATCAGTTATTTGGATGCAGCAGGAAGGATTTAGACCGGATATCGTAACTGTTGCAACGATTCTTCCAGTTTGTGCTCAATTGAGAGCTCTGAGACCCGGAAAAGAGATTCATGCTTACGCTATGAAGAATTGCTTCCTACCGAATGTATCTATCGTTTCATCTTTGATGGTTATGTACTCAAAATGTGGAGTAATGGACTATACTCTAAAGCTTTTCAACGGCATGGAGCAAAGAAATGTGATCTTATGGACAGCAATGATTGATTCGTACATAGAGAATCAATGTCCACACGAAGCAATTGATATATTCAGAGCGATGCAGCTTTCAAAGCATCGACCAGATACCGTTACCATGTCTAGAATTCTCTATATATGCAGTGAACAAAAAATGCTGAAGATGGGTAAGGAGATACATGGACAAGTCTTGAAGAGGAAATTTGAGCCGGTCCATTTCGTTTCTGCCGAGCTAGTGAAACTATATGGGAAGTGTGGAGCAGTAAAAATGGCAAAAATGGTTTTTGAGGCAATCCCTGTGAAAGGGCCAATGACATGGACTGCCATCATTGAAGCTTACGGAGAAAGTGGAGAGTTTCAGGAAGCAATCGATCTGTTTGACCGAATGAGATCACGTGGTATCTCTCCAAACCATTTCACTTTCAAAGTGGTTCTATCTATTTGCAAGGAAGCTGGTTTTGTTGATGAAGCACTGCGCATCTTTAAGCTAATGTCTGTTAGGTATAAGATTAAGCCATCTGAAGAACATTACTCTTTAGTCATTGCGATTCTTACTCGGTTTGGTCGACTCGAGGAGGCTAGAAGGTATGTACAAATGCTTTCTTCATTGTCATAA

Coding sequence (CDS)

ATGGAAATTTCTTCATCCTTCATTATCTCCCTTCATCTTCAACCTTTCACTCCCAATTCTCTCGCCCCGGCCACCGCTATTTGTAATTCAGGCCACCGACTTTCCCGAATCAAATCCACCACAGATACACCGCCGTCAAAAATCAAAATAGTTTCCAAATTTCGGAACCGAAAACGCCCAACTTTTGCCGAGAAAGATGCTTTTCCTTCATCTTTACCACTTCACACCAAGAACCCTCATGCCATTTACGAGGACGTTCAAAGATTTGCACGGCAAAATAAGCTCAAAGAGGCACTTACGATTATGGACTATGTGGATCAACAAGGCATTCCAGTTAATGCCACTACATTTTCTTCTCTTATTACGGCTTGTGTTAGAACAAAATCGATGACTTATGCGAAACAGATTCACGCGCATATTCGGATAAATGGGCTTGAAAACAATGAATTTATACGTACAAGGCTTGTGCATATGTATACTGCTTGTGGGTCCTTGGAAGAGGCACAGAAGCTGTTTGATGAAAGTTCTAGCAAGAGTGTTTATCCTTGGAATGCGTTGCTTAGAGGCACTGTAATGGCGGGTCGGAGGGATTATCGCAGCATTCTATCGACTTATGCAGAAATGCGAAGATTGGGAGTTGAATTGAACGTTTACTCCTTTGCTAATATCATTAAAAGCTTTGCAGGTGCATCGGCGTTTACTCAGGGGCTCAAGGCTCATGGCCTTTTGATTAAAAATGGATTGATTGGCAGTTCACTTCTTGGGACAACTTTGGTTGATATGTACTTCAAATGTGGTAAGATCAAACTTGCCCGCCAGATGTTTGGGGAAATTACCGAGAGAGATGTTGTGGTTTGGGGATCAATAATTGCTGGGTTTGCTCACAATCGACTCCAAAGGGAAGCTTTGGAATATACAAGGAGGATGATAGACGATGGAATTAGACCAAATTCGGTTATATTGACGACCATTCTTCCAGTTATTGGAGAAATCTGGGCGAGGAGATTAGGTCAGGAAGTTCATGCTTATGTTATAAAGACGAAAAGTTACTCGAAGCAGATATTTATTCAGTCTGCTTTGATTGATATGTACTGCAAGTGTGGGGACATCGGTTCAGGTAGAGCCGTTTTTTATGCATCCATGGAGAGGAATGCTATATGCTGGACTGCTTTGATGTCTGGTTATGCTTTAAATGGTAGGCTAGAACAGGCTGTAAGATCAGTTATTTGGATGCAGCAGGAAGGATTTAGACCGGATATCGTAACTGTTGCAACGATTCTTCCAGTTTGTGCTCAATTGAGAGCTCTGAGACCCGGAAAAGAGATTCATGCTTACGCTATGAAGAATTGCTTCCTACCGAATGTATCTATCGTTTCATCTTTGATGGTTATGTACTCAAAATGTGGAGTAATGGACTATACTCTAAAGCTTTTCAACGGCATGGAGCAAAGAAATGTGATCTTATGGACAGCAATGATTGATTCGTACATAGAGAATCAATGTCCACACGAAGCAATTGATATATTCAGAGCGATGCAGCTTTCAAAGCATCGACCAGATACCGTTACCATGTCTAGAATTCTCTATATATGCAGTGAACAAAAAATGCTGAAGATGGGTAAGGAGATACATGGACAAGTCTTGAAGAGGAAATTTGAGCCGGTCCATTTCGTTTCTGCCGAGCTAGTGAAACTATATGGGAAGTGTGGAGCAGTAAAAATGGCAAAAATGGTTTTTGAGGCAATCCCTGTGAAAGGGCCAATGACATGGACTGCCATCATTGAAGCTTACGGAGAAAGTGGAGAGTTTCAGGAAGCAATCGATCTGTTTGACCGAATGAGATCACGTGGTATCTCTCCAAACCATTTCACTTTCAAAGTGGTTCTATCTATTTGCAAGGAAGCTGGTTTTGTTGATGAAGCACTGCGCATCTTTAAGCTAATGTCTGTTAGGTATAAGATTAAGCCATCTGAAGAACATTACTCTTTAGTCATTGCGATTCTTACTCGGTTTGGTCGACTCGAGGAGGCTAGAAGGTATGTACAAATGCTTTCTTCATTGTCATAA

Protein sequence

MEISSSFIISLHLQPFTPNSLAPATAICNSGHRLSRIKSTTDTPPSKIKIVSKFRNRKRPTFAEKDAFPSSLPLHTKNPHAIYEDVQRFARQNKLKEALTIMDYVDQQGIPVNATTFSSLITACVRTKSMTYAKQIHAHIRINGLENNEFIRTRLVHMYTACGSLEEAQKLFDESSSKSVYPWNALLRGTVMAGRRDYRSILSTYAEMRRLGVELNVYSFANIIKSFAGASAFTQGLKAHGLLIKNGLIGSSLLGTTLVDMYFKCGKIKLARQMFGEITERDVVVWGSIIAGFAHNRLQREALEYTRRMIDDGIRPNSVILTTILPVIGEIWARRLGQEVHAYVIKTKSYSKQIFIQSALIDMYCKCGDIGSGRAVFYASMERNAICWTALMSGYALNGRLEQAVRSVIWMQQEGFRPDIVTVATILPVCAQLRALRPGKEIHAYAMKNCFLPNVSIVSSLMVMYSKCGVMDYTLKLFNGMEQRNVILWTAMIDSYIENQCPHEAIDIFRAMQLSKHRPDTVTMSRILYICSEQKMLKMGKEIHGQVLKRKFEPVHFVSAELVKLYGKCGAVKMAKMVFEAIPVKGPMTWTAIIEAYGESGEFQEAIDLFDRMRSRGISPNHFTFKVVLSICKEAGFVDEALRIFKLMSVRYKIKPSEEHYSLVIAILTRFGRLEEARRYVQMLSSLS*
BLAST of Cucsa.237230 vs. Swiss-Prot
Match: PP115_ARATH (Pentatricopeptide repeat-containing protein At1g71460, chloroplastic OS=Arabidopsis thaliana GN=PCMP-A3 PE=2 SV=1)

HSP 1 Score: 860.9 bits (2223), Expect = 9.4e-249
Identity = 417/674 (61.87%), Postives = 537/674 (79.67%), Query Frame = 1

Query: 18  PNSLAPATAICNSGHRLSRIKSTTDTP--PSKIKIVSKFRNRKRPTFAEKDAFPSSLPLH 77
           P SL+  T++ +  HR  +  +   +P  PS+ +  S    +K   F E+DAFPSSLPLH
Sbjct: 13  PASLSVTTSLNHRPHRSDKDGAPAKSPIRPSRTRRPSTSPAKKPKPFRERDAFPSSLPLH 72

Query: 78  TKNPHAIYEDVQRFARQNKLKEALTIMDYVDQQGIPVNATTFSSLITACVRTKSMTYAKQ 137
           +KNP+ I+ D+Q FARQN L+ ALTI+DY++Q+GIPVNATTFS+L+ ACVR KS+ + KQ
Sbjct: 73  SKNPYIIHRDIQIFARQNNLEVALTILDYLEQRGIPVNATTFSALLEACVRRKSLLHGKQ 132

Query: 138 IHAHIRINGLENNEFIRTRLVHMYTACGSLEEAQKLFDESSSKSVYPWNALLRGTVMAGR 197
           +H HIRINGLE+NEF+RT+LVHMYTACGS+++AQK+FDES+S +VY WNALLRGTV++G+
Sbjct: 133 VHVHIRINGLESNEFLRTKLVHMYTACGSVKDAQKVFDESTSSNVYSWNALLRGTVISGK 192

Query: 198 RDYRSILSTYAEMRRLGVELNVYSFANIIKSFAGASAFTQGLKAHGLLIKNGLIGSSLLG 257
           + Y+ +LST+ EMR LGV+LNVYS +N+ KSFAGASA  QGLK H L IKNGL  S  L 
Sbjct: 193 KRYQDVLSTFTEMRELGVDLNVYSLSNVFKSFAGASALRQGLKTHALAIKNGLFNSVFLK 252

Query: 258 TTLVDMYFKCGKIKLARQMFGEITERDVVVWGSIIAGFAHNRLQREALEYTRRMI-DDGI 317
           T+LVDMYFKCGK+ LAR++F EI ERD+VVWG++IAG AHN+ Q EAL   R MI ++ I
Sbjct: 253 TSLVDMYFKCGKVGLARRVFDEIVERDIVVWGAMIAGLAHNKRQWEALGLFRTMISEEKI 312

Query: 318 RPNSVILTTILPVIGEIWARRLGQEVHAYVIKTKSYSKQIFIQSALIDMYCKCGDIGSGR 377
            PNSVILTTILPV+G++ A +LG+EVHA+V+K+K+Y +Q F+ S LID+YCKCGD+ SGR
Sbjct: 313 YPNSVILTTILPVLGDVKALKLGKEVHAHVLKSKNYVEQPFVHSGLIDLYCKCGDMASGR 372

Query: 378 AVFYASMERNAICWTALMSGYALNGRLEQAVRSVIWMQQEGFRPDIVTVATILPVCAQLR 437
            VFY S +RNAI WTALMSGYA NGR +QA+RS++WMQQEGFRPD+VT+AT+LPVCA+LR
Sbjct: 373 RVFYGSKQRNAISWTALMSGYAANGRFDQALRSIVWMQQEGFRPDVVTIATVLPVCAELR 432

Query: 438 ALRPGKEIHAYAMKNCFLPNVSIVSSLMVMYSKCGVMDYTLKLFNGMEQRNVILWTAMID 497
           A++ GKEIH YA+KN FLPNVS+V+SLMVMYSKCGV +Y ++LF+ +EQRNV  WTAMID
Sbjct: 433 AIKQGKEIHCYALKNLFLPNVSLVTSLMVMYSKCGVPEYPIRLFDRLEQRNVKAWTAMID 492

Query: 498 SYIENQCPHEAIDIFRAMQLSKHRPDTVTMSRILYICSEQKMLKMGKEIHGQVLKRKFEP 557
            Y+EN      I++FR M LSKHRPD+VTM R+L +CS+ K LK+GKE+HG +LK++FE 
Sbjct: 493 CYVENCDLRAGIEVFRLMLLSKHRPDSVTMGRVLTVCSDLKALKLGKELHGHILKKEFES 552

Query: 558 VHFVSAELVKLYGKCGAVKMAKMVFEAIPVKGPMTWTAIIEAYGESGEFQEAIDLFDRMR 617
           + FVSA ++K+YGKCG ++ A   F+A+ VKG +TWTAIIEAYG +  F++AI+ F++M 
Sbjct: 553 IPFVSARIIKMYGKCGDLRSANFSFDAVAVKGSLTWTAIIEAYGCNELFRDAINCFEQMV 612

Query: 618 SRGISPNHFTFKVVLSICKEAGFVDEALRIFKLMSVRYKIKPSEEHYSLVIAILTRFGRL 677
           SRG +PN FTF  VLSIC +AGFVDEA R F LM   Y ++PSEEHYSLVI +L R GR+
Sbjct: 613 SRGFTPNTFTFTAVLSICSQAGFVDEAYRFFNLMLRMYNLQPSEEHYSLVIELLNRCGRV 672

Query: 678 EEARRYVQMLSSLS 689
           EEA+R   M SS S
Sbjct: 673 EEAQRLAVMSSSSS 686

BLAST of Cucsa.237230 vs. Swiss-Prot
Match: PP320_ARATH (Pentatricopeptide repeat-containing protein DOT4, chloroplastic OS=Arabidopsis thaliana GN=DOT4 PE=2 SV=1)

HSP 1 Score: 349.0 bits (894), Expect = 1.2e-94
Identity = 192/598 (32.11%), Postives = 330/598 (55.18%), Query Frame = 1

Query: 86  VQRFARQNKLKEALTIMDYVDQQGIPVNATTFSSLITACVRTKSMTYAKQIHAHIRINGL 145
           ++RF     L+ A+ ++    +  I  +  T  S++  C  +KS+   K++   IR NG 
Sbjct: 68  LRRFCESGNLENAVKLLCVSGKWDI--DPRTLCSVLQLCADSKSLKDGKEVDNFIRGNGF 127

Query: 146 ENNEFIRTRLVHMYTACGSLEEAQKLFDESSSKSVYPWNALLRGTVMAGRRDYRSILSTY 205
             +  + ++L  MYT CG L+EA ++FDE   +    WN L+     +G  D+   +  +
Sbjct: 128 VIDSNLGSKLSLMYTNCGDLKEASRVFDEVKIEKALFWNILMNELAKSG--DFSGSIGLF 187

Query: 206 AEMRRLGVELNVYSFANIIKSFAGASAFTQGLKAHGLLIKNGLIGSSLLGTTLVDMYFKC 265
            +M   GVE++ Y+F+ + KSF+   +   G + HG ++K+G    + +G +LV  Y K 
Sbjct: 188 KKMMSSGVEMDSYTFSCVSKSFSSLRSVHGGEQLHGFILKSGFGERNSVGNSLVAFYLKN 247

Query: 266 GKIKLARQMFGEITERDVVVWGSIIAGFAHNRLQREALEYTRRMIDDGIRPNSVILTTIL 325
            ++  AR++F E+TERDV+ W SII G+  N L  + L    +M+  GI  +   + ++ 
Sbjct: 248 QRVDSARKVFDEMTERDVISWNSIINGYVSNGLAEKGLSVFVQMLVSGIEIDLATIVSVF 307

Query: 326 PVIGEIWARRLGQEVHAYVIKTKSYSKQIFIQSALIDMYCKCGDIGSGRAVFYASMERNA 385
               +     LG+ VH+  +K   +S++    + L+DMY KCGD+ S +AVF    +R+ 
Sbjct: 308 AGCADSRLISLGRAVHSIGVKA-CFSREDRFCNTLLDMYSKCGDLDSAKAVFREMSDRSV 367

Query: 386 ICWTALMSGYALNGRLEQAVRSVIWMQQEGFRPDIVTVATILPVCAQLRALRPGKEIHAY 445
           + +T++++GYA  G   +AV+    M++EG  PD+ TV  +L  CA+ R L  GK +H +
Sbjct: 368 VSYTSMIAGYAREGLAGEAVKLFEEMEEEGISPDVYTVTAVLNCCARYRLLDEGKRVHEW 427

Query: 446 AMKNCFLPNVSIVSSLMVMYSKCGVMDYTLKLFNGMEQRNVILWTAMIDSYIENQCPHEA 505
             +N    ++ + ++LM MY+KCG M     +F+ M  +++I W  +I  Y +N   +EA
Sbjct: 428 IKENDLGFDIFVSNALMDMYAKCGSMQEAELVFSEMRVKDIISWNTIIGGYSKNCYANEA 487

Query: 506 IDIFRAMQLSKH-RPDTVTMSRILYICSEQKMLKMGKEIHGQVLKRKFEPVHFVSAELVK 565
           + +F  +   K   PD  T++ +L  C+       G+EIHG +++  +     V+  LV 
Sbjct: 488 LSLFNLLLEEKRFSPDERTVACVLPACASLSAFDKGREIHGYIMRNGYFSDRHVANSLVD 547

Query: 566 LYGKCGAVKMAKMVFEAIPVKGPMTWTAIIEAYGESGEFQEAIDLFDRMRSRGISPNHFT 625
           +Y KCGA+ +A M+F+ I  K  ++WT +I  YG  G  +EAI LF++MR  GI  +  +
Sbjct: 548 MYAKCGALLLAHMLFDDIASKDLVSWTVMIAGYGMHGFGKEAIALFNQMRQAGIEADEIS 607

Query: 626 FKVVLSICKEAGFVDEALRIFKLMSVRYKIKPSEEHYSLVIAILTRFGRLEEARRYVQ 683
           F  +L  C  +G VDE  R F +M    KI+P+ EHY+ ++ +L R G L +A R+++
Sbjct: 608 FVSLLYACSHSGLVDEGWRFFNIMRHECKIEPTVEHYACIVDMLARTGDLIKAYRFIE 660

BLAST of Cucsa.237230 vs. Swiss-Prot
Match: PP285_ARATH (Pentatricopeptide repeat-containing protein At3g57430, chloroplastic OS=Arabidopsis thaliana GN=PCMP-H81 PE=2 SV=2)

HSP 1 Score: 344.4 bits (882), Expect = 3.0e-93
Identity = 206/668 (30.84%), Postives = 346/668 (51.80%), Query Frame = 1

Query: 45  PSKIKIVSKFRN----RKRPTFAEKDAF------PSSLPLHTKNPHAIYEDVQRFARQNK 104
           PS++   S+ ++    R  PT A +D        PS     +++P    + ++   R N 
Sbjct: 18  PSQLLPFSRHKHPYLLRATPTSATEDVASAVSGAPSIFISQSRSPEWWIDLLRSKVRSNL 77

Query: 105 LKEALTIMDYVDQ--QGIPVNATTFSSLITACVRTKSMTYAKQIHAHIRINGLE-NNEFI 164
           L+EA  ++ YVD    GI  +   F +L+ A    + M   KQIHAH+   G   ++  +
Sbjct: 78  LREA--VLTYVDMIVLGIKPDNYAFPALLKAVADLQDMELGKQIHAHVYKFGYGVDSVTV 137

Query: 165 RTRLVHMYTACGSLEEAQKLFDESSSKSVYPWNALLRGTVMAGRRDYRSILSTYAEMRRL 224
              LV++Y  CG      K+FD  S ++   WN+L+    +     +   L  +  M   
Sbjct: 138 ANTLVNLYRKCGDFGAVYKVFDRISERNQVSWNSLISS--LCSFEKWEMALEAFRCMLDE 197

Query: 225 GVELNVYSFANIIKSFAGA---SAFTQGLKAHGLLIKNGLIGSSLLGTTLVDMYFKCGKI 284
            VE + ++  +++ + +          G + H   ++ G + S ++ T LV MY K GK+
Sbjct: 198 NVEPSSFTLVSVVTACSNLPMPEGLMMGKQVHAYGLRKGELNSFIINT-LVAMYGKLGKL 257

Query: 285 KLARQMFGEITERDVVVWGSIIAGFAHNRLQREALEYTRRMIDDGIRPNSVILTTILPVI 344
             ++ + G    RD+V W ++++    N    EALEY R M+ +G+ P+   ++++LP  
Sbjct: 258 ASSKVLLGSFGGRDLVTWNTVLSSLCQNEQLLEALEYLREMVLEGVEPDEFTISSVLPAC 317

Query: 345 GEIWARRLGQEVHAYVIKTKSYSKQIFIQSALIDMYCKCGDIGSGRAVFYASMERNAICW 404
             +   R G+E+HAY +K  S  +  F+ SAL+DMYC C  + SGR VF    +R    W
Sbjct: 318 SHLEMLRTGKELHAYALKNGSLDENSFVGSALVDMYCNCKQVLSGRRVFDGMFDRKIGLW 377

Query: 405 TALMSGYALNGRLEQAVRSVIWMQQE-GFRPDIVTVATILPVCAQLRALRPGKEIHAYAM 464
            A+++GY+ N   ++A+   I M++  G   +  T+A ++P C +  A    + IH + +
Sbjct: 378 NAMIAGYSQNEHDKEALLLFIGMEESAGLLANSTTMAGVVPACVRSGAFSRKEAIHGFVV 437

Query: 465 KNCFLPNVSIVSSLMVMYSKCGVMDYTLKLFNGMEQRNVILWTAMIDSYIENQCPHEAID 524
           K     +  + ++LM MYS+ G +D  +++F  ME R+++ W  MI  Y+ ++   +A+ 
Sbjct: 438 KRGLDRDRFVQNTLMDMYSRLGKIDIAMRIFGKMEDRDLVTWNTMITGYVFSEHHEDALL 497

Query: 525 IFRAMQ-----LSKH------RPDTVTMSRILYICSEQKMLKMGKEIHGQVLKRKFEPVH 584
           +   MQ     +SK       +P+++T+  IL  C+    L  GKEIH   +K       
Sbjct: 498 LLHKMQNLERKVSKGASRVSLKPNSITLMTILPSCAALSALAKGKEIHAYAIKNNLATDV 557

Query: 585 FVSAELVKLYGKCGAVKMAKMVFEAIPVKGPMTWTAIIEAYGESGEFQEAIDLFDRMRSR 644
            V + LV +Y KCG ++M++ VF+ IP K  +TW  II AYG  G  QEAIDL   M  +
Sbjct: 558 AVGSALVDMYAKCGCLQMSRKVFDQIPQKNVITWNVIIMAYGMHGNGQEAIDLLRMMMVQ 617

Query: 645 GISPNHFTFKVVLSICKEAGFVDEALRIFKLMSVRYKIKPSEEHYSLVIAILTRFGRLEE 685
           G+ PN  TF  V + C  +G VDE LRIF +M   Y ++PS +HY+ V+ +L R GR++E
Sbjct: 618 GVKPNEVTFISVFAACSHSGMVDEGLRIFYVMKPDYGVEPSSDHYACVVDLLGRAGRIKE 677


HSP 2 Score: 70.5 bits (171), Expect = 8.3e-11
Identity = 54/225 (24.00%), Postives = 105/225 (46.67%), Query Frame = 1

Query: 113 NATTFSSLITACVRTKSMTYAKQIHAHIRINGLENNEFIRTRLVHMYTACGSLEEAQKLF 172
           N+ T  +++ +C    ++   K+IHA+   N L  +  + + LV MY  CG L+ ++K+F
Sbjct: 516 NSITLMTILPSCAALSALAKGKEIHAYAIKNNLATDVAVGSALVDMYAKCGCLQMSRKVF 575

Query: 173 DESSSKSVYPWNALLRGTVMAGRRDYRSILSTYAEMRRLGVELNVYSFANIIKSFAGASA 232
           D+   K+V  WN ++    M G  + +  +     M   GV+ N  +F ++  + + +  
Sbjct: 576 DQIPQKNVITWNVIIMAYGMHG--NGQEAIDLLRMMMVQGVKPNEVTFISVFAACSHSGM 635

Query: 233 FTQGLKAHGLLIKN-GLIGSSLLGTTLVDMYFKCGKIKLARQMFGEITE--RDVVVWGSI 292
             +GL+   ++  + G+  SS     +VD+  + G+IK A Q+   +         W S+
Sbjct: 636 VDEGLRIFYVMKPDYGVEPSSDHYACVVDLLGRAGRIKEAYQLMNMMPRDFNKAGAWSSL 695

Query: 293 I-AGFAHNRLQ-REALEYTRRMIDDGIRPNSVILTTILPVIGEIW 333
           + A   HN L+  E        ++  +  + V+L  I    G +W
Sbjct: 696 LGASRIHNNLEIGEIAAQNLIQLEPNVASHYVLLANIYSSAG-LW 737

BLAST of Cucsa.237230 vs. Swiss-Prot
Match: PPR32_ARATH (Pentatricopeptide repeat-containing protein At1g11290, chloroplastic OS=Arabidopsis thaliana GN=PCMP-H40 PE=2 SV=1)

HSP 1 Score: 334.7 bits (857), Expect = 2.3e-90
Identity = 179/559 (32.02%), Postives = 307/559 (54.92%), Query Frame = 1

Query: 126 RTKSMTYAKQIHAHIRINGLENNEFIRTRLVHMYTACGSLEEAQKLFDESSSKSVYPWNA 185
           R  S+   +QI   +  NGL    F +T+LV ++   GS++EA ++F+   SK    ++ 
Sbjct: 46  RCSSLKELRQILPLVFKNGLYQEHFFQTKLVSLFCRYGSVDEAARVFEPIDSKLNVLYHT 105

Query: 186 LLRGTVMAGRRDYRSILSTYAEMRRLGVELNVYSFANIIKSFAGASAFTQGLKAHGLLIK 245
           +L+G   A   D    L  +  MR   VE  VY+F  ++K     +    G + HGLL+K
Sbjct: 106 MLKG--FAKVSDLDKALQFFVRMRYDDVEPVVYNFTYLLKVCGDEAELRVGKEIHGLLVK 165

Query: 246 NGLIGSSLLGTTLVDMYFKCGKIKLARQMFGEITERDVVVWGSIIAGFAHNRLQREALEY 305
           +G        T L +MY KC ++  AR++F  + ERD+V W +I+AG++ N + R ALE 
Sbjct: 166 SGFSLDLFAMTGLENMYAKCRQVNEARKVFDRMPERDLVSWNTIVAGYSQNGMARMALEM 225

Query: 306 TRRMIDDGIRPNSVILTTILPVIGEIWARRLGQEVHAYVIKTKSYSKQIFIQSALIDMYC 365
            + M ++ ++P+ + + ++LP +  +    +G+E+H Y +++  +   + I +AL+DMY 
Sbjct: 226 VKSMCEENLKPSFITIVSVLPAVSALRLISVGKEIHGYAMRS-GFDSLVNISTALVDMYA 285

Query: 366 KCGDIGSGRAVFYASMERNAICWTALMSGYALNGRLEQAVRSVIWMQQEGFRPDIVTVAT 425
           KCG + + R +F   +ERN + W +++  Y  N   ++A+     M  EG +P  V+V  
Sbjct: 286 KCGSLETARQLFDGMLERNVVSWNSMIDAYVQNENPKEAMLIFQKMLDEGVKPTDVSVMG 345

Query: 426 ILPVCAQLRALRPGKEIHAYAMKNCFLPNVSIVSSLMVMYSKCGVMDYTLKLFNGMEQRN 485
            L  CA L  L  G+ IH  +++     NVS+V+SL+ MY KC  +D    +F  ++ R 
Sbjct: 346 ALHACADLGDLERGRFIHKLSVELGLDRNVSVVNSLISMYCKCKEVDTAASMFGKLQSRT 405

Query: 486 VILWTAMIDSYIENQCPHEAIDIFRAMQLSKHRPDTVTMSRILYICSEQKMLKMGKEIHG 545
           ++ W AMI  + +N  P +A++ F  M+    +PDT T   ++   +E  +    K IHG
Sbjct: 406 LVSWNAMILGFAQNGRPIDALNYFSQMRSRTVKPDTFTYVSVITAIAELSITHHAKWIHG 465

Query: 546 QVLKRKFEPVHFVSAELVKLYGKCGAVKMAKMVFEAIPVKGPMTWTAIIEAYGESGEFQE 605
            V++   +   FV+  LV +Y KCGA+ +A+++F+ +  +   TW A+I+ YG  G  + 
Sbjct: 466 VVMRSCLDKNVFVTTALVDMYAKCGAIMIARLIFDMMSERHVTTWNAMIDGYGTHGFGKA 525

Query: 606 AIDLFDRMRSRGISPNHFTFKVVLSICKEAGFVDEALRIFKLMSVRYKIKPSEEHYSLVI 665
           A++LF+ M+   I PN  TF  V+S C  +G V+  L+ F +M   Y I+ S +HY  ++
Sbjct: 526 ALELFEEMQKGTIKPNGVTFLSVISACSHSGLVEAGLKCFYMMKENYSIELSMDHYGAMV 585

Query: 666 AILTRFGRLEEARRYVQML 685
            +L R GRL EA  ++  +
Sbjct: 586 DLLGRAGRLNEAWDFIMQM 601


HSP 2 Score: 224.6 bits (571), Expect = 3.4e-57
Identity = 136/519 (26.20%), Postives = 251/519 (48.36%), Query Frame = 1

Query: 73  PLHTKNPHAIYEDVQRFARQNKLKEALTI---MDYVDQQGIPVNATTFSSLITACVRTKS 132
           P+ +K     +  ++ FA+ + L +AL     M Y D + +  N   F+ L+  C     
Sbjct: 94  PIDSKLNVLYHTMLKGFAKVSDLDKALQFFVRMRYDDVEPVVYN---FTYLLKVCGDEAE 153

Query: 133 MTYAKQIHAHIRINGLENNEFIRTRLVHMYTACGSLEEAQKLFDESSSKSVYPWNALLRG 192
           +   K+IH  +  +G   + F  T L +MY  C  + EA+K+FD    + +  WN ++ G
Sbjct: 154 LRVGKEIHGLLVKSGFSLDLFAMTGLENMYAKCRQVNEARKVFDRMPERDLVSWNTIVAG 213

Query: 193 TVMAGRRDYRSILSTYAEMRRLGVELNVYSFANIIKSFAGASAFTQGLKAHGLLIKNGLI 252
               G    R  L     M    ++ +  +  +++ + +     + G + HG  +++G  
Sbjct: 214 YSQNGMA--RMALEMVKSMCEENLKPSFITIVSVLPAVSALRLISVGKEIHGYAMRSGFD 273

Query: 253 GSSLLGTTLVDMYFKCGKIKLARQMFGEITERDVVVWGSIIAGFAHNRLQREALEYTRRM 312
               + T LVDMY KCG ++ ARQ+F  + ER+VV W S+I  +  N   +EA+   ++M
Sbjct: 274 SLVNISTALVDMYAKCGSLETARQLFDGMLERNVVSWNSMIDAYVQNENPKEAMLIFQKM 333

Query: 313 IDDGIRPNSVILTTILPVIGEIWARRLGQEVHAYVIKTKSYSKQIFIQSALIDMYCKCGD 372
           +D+G++P  V +   L    ++     G+ +H   ++     + + + ++LI MYCKC +
Sbjct: 334 LDEGVKPTDVSVMGALHACADLGDLERGRFIHKLSVEL-GLDRNVSVVNSLISMYCKCKE 393

Query: 373 IGSGRAVFYASMERNAICWTALMSGYALNGRLEQAVRSVIWMQQEGFRPDIVTVATILPV 432
           + +  ++F     R  + W A++ G+A NGR   A+     M+    +PD  T  +++  
Sbjct: 394 VDTAASMFGKLQSRTLVSWNAMILGFAQNGRPIDALNYFSQMRSRTVKPDTFTYVSVITA 453

Query: 433 CAQLRALRPGKEIHAYAMKNCFLPNVSIVSSLMVMYSKCGVMDYTLKLFNGMEQRNVILW 492
            A+L      K IH   M++C   NV + ++L+ MY+KCG +     +F+ M +R+V  W
Sbjct: 454 IAELSITHHAKWIHGVVMRSCLDKNVFVTTALVDMYAKCGAIMIARLIFDMMSERHVTTW 513

Query: 493 TAMIDSYIENQCPHEAIDIFRAMQLSKHRPDTVTMSRILYICSEQKMLKMGKEIHGQVLK 552
            AMID Y  +     A+++F  MQ    +P+ VT   ++  CS   +++ G +    + +
Sbjct: 514 NAMIDGYGTHGFGKAALELFEEMQKGTIKPNGVTFLSVISACSHSGLVEAGLKCFYMMKE 573

Query: 553 R---KFEPVHFVSAELVKLYGKCGAVKMAKMVFEAIPVK 586
               +    H+    +V L G+ G +  A      +PVK
Sbjct: 574 NYSIELSMDHY--GAMVDLLGRAGRLNEAWDFIMQMPVK 604

BLAST of Cucsa.237230 vs. Swiss-Prot
Match: PP333_ARATH (Pentatricopeptide repeat-containing protein At4g21300 OS=Arabidopsis thaliana GN=PCMP-E36 PE=3 SV=1)

HSP 1 Score: 324.7 bits (831), Expect = 2.4e-87
Identity = 180/600 (30.00%), Postives = 313/600 (52.17%), Query Frame = 1

Query: 86  VQRFARQNKLKEALTIMDYVDQQGIPVNATTFSSLITACVRTKSMTYAKQIHAHIRINGL 145
           +  F R   L +AL     +   G+  + +TF  L+ ACV  K+      +   +   G+
Sbjct: 110 ISSFVRNGLLNQALAFYFKMLCFGVSPDVSTFPCLVKACVALKNFKGIDFLSDTVSSLGM 169

Query: 146 ENNEFIRTRLVHMYTACGSLEEAQKLFDESSSKSVYPWNALLRGTVMAGRRDYRSILSTY 205
           + NEF+ + L+  Y   G ++   KLFD    K    WN +L G    G  D  S++  +
Sbjct: 170 DCNEFVASSLIKAYLEYGKIDVPSKLFDRVLQKDCVIWNVMLNGYAKCGALD--SVIKGF 229

Query: 206 AEMRRLGVELNVYSFANIIKSFAGASAFTQGLKAHGLLIKNGLIGSSLLGTTLVDMYFKC 265
           + MR   +  N  +F  ++   A       G++ HGL++ +G+     +  +L+ MY KC
Sbjct: 230 SVMRMDQISPNAVTFDCVLSVCASKLLIDLGVQLHGLVVVSGVDFEGSIKNSLLSMYSKC 289

Query: 266 GKIKLARQMFGEITERDVVVWGSIIAGFAHNRLQREALEYTRRMIDDGIRPNSVILTTIL 325
           G+   A ++F  ++  D V W  +I+G+  + L  E+L +   MI  G+ P+++  +++L
Sbjct: 290 GRFDDASKLFRMMSRADTVTWNCMISGYVQSGLMEESLTFFYEMISSGVLPDAITFSSLL 349

Query: 326 PVIGEIWARRLGQEVHAYVIKTKSYSKQIFIQSALIDMYCKCGDIGSGRAVFYASMERNA 385
           P + +       +++H Y+++  S S  IF+ SALID Y KC  +   + +F      + 
Sbjct: 350 PSVSKFENLEYCKQIHCYIMR-HSISLDIFLTSALIDAYFKCRGVSMAQNIFSQCNSVDV 409

Query: 386 ICWTALMSGYALNGRLEQAVRSVIWMQQEGFRPDIVTVATILPVCAQLRALRPGKEIHAY 445
           + +TA++SGY  NG    ++    W+ +    P+ +T+ +ILPV   L AL+ G+E+H +
Sbjct: 410 VVFTAMISGYLHNGLYIDSLEMFRWLVKVKISPNEITLVSILPVIGILLALKLGRELHGF 469

Query: 446 AMKNCFLPNVSIVSSLMVMYSKCGVMDYTLKLFNGMEQRNVILWTAMIDSYIENQCPHEA 505
            +K  F    +I  +++ MY+KCG M+   ++F  + +R+++ W +MI    ++  P  A
Sbjct: 470 IIKKGFDNRCNIGCAVIDMYAKCGRMNLAYEIFERLSKRDIVSWNSMITRCAQSDNPSAA 529

Query: 506 IDIFRAMQLSKHRPDTVTMSRILYICSEQKMLKMGKEIHGQVLKRKFEPVHFVSAELVKL 565
           IDIFR M +S    D V++S  L  C+       GK IHG ++K       +  + L+ +
Sbjct: 530 IDIFRQMGVSGICYDCVSISAALSACANLPSESFGKAIHGFMIKHSLASDVYSESTLIDM 589

Query: 566 YGKCGAVKMAKMVFEAIPVKGPMTWTAIIEAYGESGEFQEAIDLFDRM-RSRGISPNHFT 625
           Y KCG +K A  VF+ +  K  ++W +II A G  G+ ++++ LF  M    GI P+  T
Sbjct: 590 YAKCGNLKAAMNVFKTMKEKNIVSWNSIIAACGNHGKLKDSLCLFHEMVEKSGIRPDQIT 649

Query: 626 FKVVLSICKEAGFVDEALRIFKLMSVRYKIKPSEEHYSLVIAILTRFGRLEEARRYVQML 685
           F  ++S C   G VDE +R F+ M+  Y I+P +EHY+ V+ +  R GRL EA   V+ +
Sbjct: 650 FLEIISSCCHVGDVDEGVRFFRSMTEDYGIQPQQEHYACVVDLFGRAGRLTEAYETVKSM 706


HSP 2 Score: 224.6 bits (571), Expect = 3.4e-57
Identity = 131/517 (25.34%), Postives = 253/517 (48.94%), Query Frame = 1

Query: 118 SSLITACVRTKSMTYAKQIHAHIRINGLENNEFIRTRLVHMYTACGSLEEAQKLFDESSS 177
           S L+ AC     +   KQ+HA + +N +  + +   R++ MY  CGS  +  K+F     
Sbjct: 39  SLLLQACSNPNLLRQGKQVHAFLIVNSISGDSYTDERILGMYAMCGSFSDCGKMFYRLDL 98

Query: 178 K--SVYPWNALLRGTVMAGRRDYRSILSTYAEMRRLGVELNVYSFANIIKSFAGASAFTQ 237
           +  S+ PWN+++   V  G  +    L+ Y +M   GV  +V +F  ++K+      F  
Sbjct: 99  RRSSIRPWNSIISSFVRNGLLN--QALAFYFKMLCFGVSPDVSTFPCLVKACVALKNFKG 158

Query: 238 GLKAHGLLIKNGLIGSSLLGTTLVDMYFKCGKIKLARQMFGEITERDVVVWGSIIAGFAH 297
                  +   G+  +  + ++L+  Y + GKI +  ++F  + ++D V+W  ++ G+A 
Sbjct: 159 IDFLSDTVSSLGMDCNEFVASSLIKAYLEYGKIDVPSKLFDRVLQKDCVIWNVMLNGYAK 218

Query: 298 NRLQREALEYTRRMIDDGIRPNSVILTTILPVIGEIWARRLGQEVHAYVIKTKSYSKQIF 357
                  ++    M  D I PN+V    +L V        LG ++H  V+       +  
Sbjct: 219 CGALDSVIKGFSVMRMDQISPNAVTFDCVLSVCASKLLIDLGVQLHGLVV-VSGVDFEGS 278

Query: 358 IQSALIDMYCKCGDIGSGRAVFYASMERNAICWTALMSGYALNGRLEQAVRSVIWMQQEG 417
           I+++L+ MY KCG       +F      + + W  ++SGY  +G +E+++     M   G
Sbjct: 279 IKNSLLSMYSKCGRFDDASKLFRMMSRADTVTWNCMISGYVQSGLMEESLTFFYEMISSG 338

Query: 418 FRPDIVTVATILPVCAQLRALRPGKEIHAYAMKNCFLPNVSIVSSLMVMYSKCGVMDYTL 477
             PD +T +++LP  ++   L   K+IH Y M++    ++ + S+L+  Y KC  +    
Sbjct: 339 VLPDAITFSSLLPSVSKFENLEYCKQIHCYIMRHSISLDIFLTSALIDAYFKCRGVSMAQ 398

Query: 478 KLFNGMEQRNVILWTAMIDSYIENQCPHEAIDIFRAMQLSKHRPDTVTMSRILYICSEQK 537
            +F+     +V+++TAMI  Y+ N    +++++FR +   K  P+ +T+  IL +     
Sbjct: 399 NIFSQCNSVDVVVFTAMISGYLHNGLYIDSLEMFRWLVKVKISPNEITLVSILPVIGILL 458

Query: 538 MLKMGKEIHGQVLKRKFEPVHFVSAELVKLYGKCGAVKMAKMVFEAIPVKGPMTWTAIIE 597
            LK+G+E+HG ++K+ F+    +   ++ +Y KCG + +A  +FE +  +  ++W ++I 
Sbjct: 459 ALKLGRELHGFIIKKGFDNRCNIGCAVIDMYAKCGRMNLAYEIFERLSKRDIVSWNSMIT 518

Query: 598 AYGESGEFQEAIDLFDRMRSRGISPNHFTFKVVLSIC 633
              +S     AID+F +M   GI  +  +    LS C
Sbjct: 519 RCAQSDNPSAAIDIFRQMGVSGICYDCVSISAALSAC 552


HSP 3 Score: 95.1 bits (235), Expect = 3.1e-18
Identity = 64/265 (24.15%), Postives = 121/265 (45.66%), Query Frame = 1

Query: 423 VATILPVCAQLRALRPGKEIHAYAMKNCFLPNVSIVSSLMVMYSKCGVMDYTLKLFNGME 482
           ++ +L  C+    LR GK++HA+ + N    +      ++ MY+ CG      K+F  ++
Sbjct: 38  LSLLLQACSNPNLLRQGKQVHAFLIVNSISGDSYTDERILGMYAMCGSFSDCGKMFYRLD 97

Query: 483 QRNVIL--WTAMIDSYIENQCPHEAIDIFRAMQLSKHRPDTVTMSRILYICSEQKMLKMG 542
            R   +  W ++I S++ N   ++A+  +  M      PD  T   ++  C   K  K  
Sbjct: 98  LRRSSIRPWNSIISSFVRNGLLNQALAFYFKMLCFGVSPDVSTFPCLVKACVALKNFKGI 157

Query: 543 KEIHGQVLKRKFEPVHFVSAELVKLYGKCGAVKMAKMVFEAIPVKGPMTWTAIIEAYGES 602
             +   V     +   FV++ L+K Y + G + +   +F+ +  K  + W  ++  Y + 
Sbjct: 158 DFLSDTVSSLGMDCNEFVASSLIKAYLEYGKIDVPSKLFDRVLQKDCVIWNVMLNGYAKC 217

Query: 603 GEFQEAIDLFDRMRSRGISPNHFTFKVVLSICKEAGFVDEALRIFKLMSVRYKIKPSEEH 662
           G     I  F  MR   ISPN  TF  VLS+C     +D  +++  L+ V          
Sbjct: 218 GALDSVIKGFSVMRMDQISPNAVTFDCVLSVCASKLLIDLGVQLHGLVVVSGVDFEGSIK 277

Query: 663 YSLVIAILTRFGRLEEARRYVQMLS 686
            SL +++ ++ GR ++A +  +M+S
Sbjct: 278 NSL-LSMYSKCGRFDDASKLFRMMS 301

BLAST of Cucsa.237230 vs. TrEMBL
Match: A0A0A0KXW0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G649310 PE=4 SV=1)

HSP 1 Score: 1365.1 bits (3532), Expect = 0.0e+00
Identity = 688/688 (100.00%), Postives = 688/688 (100.00%), Query Frame = 1

Query: 1   MEISSSFIISLHLQPFTPNSLAPATAICNSGHRLSRIKSTTDTPPSKIKIVSKFRNRKRP 60
           MEISSSFIISLHLQPFTPNSLAPATAICNSGHRLSRIKSTTDTPPSKIKIVSKFRNRKRP
Sbjct: 1   MEISSSFIISLHLQPFTPNSLAPATAICNSGHRLSRIKSTTDTPPSKIKIVSKFRNRKRP 60

Query: 61  TFAEKDAFPSSLPLHTKNPHAIYEDVQRFARQNKLKEALTIMDYVDQQGIPVNATTFSSL 120
           TFAEKDAFPSSLPLHTKNPHAIYEDVQRFARQNKLKEALTIMDYVDQQGIPVNATTFSSL
Sbjct: 61  TFAEKDAFPSSLPLHTKNPHAIYEDVQRFARQNKLKEALTIMDYVDQQGIPVNATTFSSL 120

Query: 121 ITACVRTKSMTYAKQIHAHIRINGLENNEFIRTRLVHMYTACGSLEEAQKLFDESSSKSV 180
           ITACVRTKSMTYAKQIHAHIRINGLENNEFIRTRLVHMYTACGSLEEAQKLFDESSSKSV
Sbjct: 121 ITACVRTKSMTYAKQIHAHIRINGLENNEFIRTRLVHMYTACGSLEEAQKLFDESSSKSV 180

Query: 181 YPWNALLRGTVMAGRRDYRSILSTYAEMRRLGVELNVYSFANIIKSFAGASAFTQGLKAH 240
           YPWNALLRGTVMAGRRDYRSILSTYAEMRRLGVELNVYSFANIIKSFAGASAFTQGLKAH
Sbjct: 181 YPWNALLRGTVMAGRRDYRSILSTYAEMRRLGVELNVYSFANIIKSFAGASAFTQGLKAH 240

Query: 241 GLLIKNGLIGSSLLGTTLVDMYFKCGKIKLARQMFGEITERDVVVWGSIIAGFAHNRLQR 300
           GLLIKNGLIGSSLLGTTLVDMYFKCGKIKLARQMFGEITERDVVVWGSIIAGFAHNRLQR
Sbjct: 241 GLLIKNGLIGSSLLGTTLVDMYFKCGKIKLARQMFGEITERDVVVWGSIIAGFAHNRLQR 300

Query: 301 EALEYTRRMIDDGIRPNSVILTTILPVIGEIWARRLGQEVHAYVIKTKSYSKQIFIQSAL 360
           EALEYTRRMIDDGIRPNSVILTTILPVIGEIWARRLGQEVHAYVIKTKSYSKQIFIQSAL
Sbjct: 301 EALEYTRRMIDDGIRPNSVILTTILPVIGEIWARRLGQEVHAYVIKTKSYSKQIFIQSAL 360

Query: 361 IDMYCKCGDIGSGRAVFYASMERNAICWTALMSGYALNGRLEQAVRSVIWMQQEGFRPDI 420
           IDMYCKCGDIGSGRAVFYASMERNAICWTALMSGYALNGRLEQAVRSVIWMQQEGFRPDI
Sbjct: 361 IDMYCKCGDIGSGRAVFYASMERNAICWTALMSGYALNGRLEQAVRSVIWMQQEGFRPDI 420

Query: 421 VTVATILPVCAQLRALRPGKEIHAYAMKNCFLPNVSIVSSLMVMYSKCGVMDYTLKLFNG 480
           VTVATILPVCAQLRALRPGKEIHAYAMKNCFLPNVSIVSSLMVMYSKCGVMDYTLKLFNG
Sbjct: 421 VTVATILPVCAQLRALRPGKEIHAYAMKNCFLPNVSIVSSLMVMYSKCGVMDYTLKLFNG 480

Query: 481 MEQRNVILWTAMIDSYIENQCPHEAIDIFRAMQLSKHRPDTVTMSRILYICSEQKMLKMG 540
           MEQRNVILWTAMIDSYIENQCPHEAIDIFRAMQLSKHRPDTVTMSRILYICSEQKMLKMG
Sbjct: 481 MEQRNVILWTAMIDSYIENQCPHEAIDIFRAMQLSKHRPDTVTMSRILYICSEQKMLKMG 540

Query: 541 KEIHGQVLKRKFEPVHFVSAELVKLYGKCGAVKMAKMVFEAIPVKGPMTWTAIIEAYGES 600
           KEIHGQVLKRKFEPVHFVSAELVKLYGKCGAVKMAKMVFEAIPVKGPMTWTAIIEAYGES
Sbjct: 541 KEIHGQVLKRKFEPVHFVSAELVKLYGKCGAVKMAKMVFEAIPVKGPMTWTAIIEAYGES 600

Query: 601 GEFQEAIDLFDRMRSRGISPNHFTFKVVLSICKEAGFVDEALRIFKLMSVRYKIKPSEEH 660
           GEFQEAIDLFDRMRSRGISPNHFTFKVVLSICKEAGFVDEALRIFKLMSVRYKIKPSEEH
Sbjct: 601 GEFQEAIDLFDRMRSRGISPNHFTFKVVLSICKEAGFVDEALRIFKLMSVRYKIKPSEEH 660

Query: 661 YSLVIAILTRFGRLEEARRYVQMLSSLS 689
           YSLVIAILTRFGRLEEARRYVQMLSSLS
Sbjct: 661 YSLVIAILTRFGRLEEARRYVQMLSSLS 688

BLAST of Cucsa.237230 vs. TrEMBL
Match: W9T1A9_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_001633 PE=4 SV=1)

HSP 1 Score: 951.8 bits (2459), Expect = 4.5e-274
Identity = 454/648 (70.06%), Postives = 552/648 (85.19%), Query Frame = 1

Query: 41  TDTPPSKIKIVSKFRNRKRPTFAEKDAFPSSLPLHTKNPHAIYEDVQRFARQNKLKEALT 100
           T TP S  K     R RKRP F +KDAFP SLPLH+KNP A+Y D+QRFARQNKL +ALT
Sbjct: 5   TRTPASPGK-----RRRKRPVFTKKDAFPESLPLHSKNPRAVYSDIQRFARQNKLSQALT 64

Query: 101 IMDYVDQQGIPVNATTFSSLITACVRTKSMTYAKQIHAHIRINGLENNEFIRTRLVHMYT 160
           I+DY+DQQGIPVN TTF++LI ACVRTKS+ + KQ+HA IRINGL+ NEF+RT+LVHMYT
Sbjct: 65  ILDYMDQQGIPVNPTTFAALIAACVRTKSLDHGKQVHAFIRINGLDKNEFLRTKLVHMYT 124

Query: 161 ACGSLEEAQKLFDESSSKSVYPWNALLRGTVMAGRRDYRSILSTYAEMRRLGVELNVYSF 220
           +CGS+++A  LFDES S+SVYPWNALLRG V++G R YR  LSTY +MR LG+E+NVYSF
Sbjct: 125 SCGSVDDANNLFDESPSRSVYPWNALLRGNVISGGRRYRDALSTYYQMRALGIEMNVYSF 184

Query: 221 ANIIKSFAGASAFTQGLKAHGLLIKNGLIGSSLLGTTLVDMYFKCGKIKLARQMFGEITE 280
           +++IKS AGASA  QGLK H LLIKNGL+GS++L T+L+DMYFKCGKIKLARQ+F EI E
Sbjct: 185 SSVIKSLAGASALLQGLKTHALLIKNGLVGSAMLRTSLIDMYFKCGKIKLARQVFEEIVE 244

Query: 281 RDVVVWGSIIAGFAHNRLQREALEYTRRMIDDGIRPNSVILTTILPVIGEIWARRLGQEV 340
           RD+V WG++I+GFAHNRLQ +AL+YTRRM+D+GI+ NSVILT ILPVIGE+ AR+LG+EV
Sbjct: 245 RDIVAWGAMISGFAHNRLQWQALDYTRRMVDEGIKLNSVILTIILPVIGELLARKLGREV 304

Query: 341 HAYVIKTKSYSKQIFIQSALIDMYCKCGDIGSGRAVFYASMERNAICWTALMSGYALNGR 400
           HAY +KTK Y+KQ FIQS LIDMYCKCGD+ +GR VFY   ERNAICWTAL+SGY  NGR
Sbjct: 305 HAYAVKTKRYAKQTFIQSGLIDMYCKCGDMENGRRVFYRLKERNAICWTALISGYVANGR 364

Query: 401 LEQAVRSVIWMQQEGFRPDIVTVATILPVCAQLRALRPGKEIHAYAMKNCFLPNVSIVSS 460
           LEQA+RS+IWMQQEG RPD+VTVAT++P+CA+LRAL+PGKEIHAYA+KNCFLPNVSIVSS
Sbjct: 365 LEQALRSIIWMQQEGIRPDVVTVATVVPICAELRALKPGKEIHAYAVKNCFLPNVSIVSS 424

Query: 461 LMVMYSKCGVMDYTLKLFNGMEQRNVILWTAMIDSYIENQCPHEAIDIFRAMQLSKHRPD 520
           LM+MYSKCGV+DY+++LF GMEQRNVILWTAMIDSY+EN+   EA+ + R+M LSKHRPD
Sbjct: 425 LMMMYSKCGVLDYSVRLFEGMEQRNVILWTAMIDSYVENRHLDEALSVIRSMVLSKHRPD 484

Query: 521 TVTMSRILYICSEQKMLKMGKEIHGQVLKRKFEPVHFVSAELVKLYGKCGAVKMAKMVFE 580
           +V + R+L IC+E K LK GKEIHGQVLKR FE VHFVSAE+VK+YG+CG +  AK+VF+
Sbjct: 485 SVAIGRMLCICNELKSLKFGKEIHGQVLKRNFESVHFVSAEIVKMYGRCGVIDDAKLVFD 544

Query: 581 AIPVKGPMTWTAIIEAYGESGEFQEAIDLFDRMRSRGISPNHFTFKVVLSICKEAGFVDE 640
            I VKG MTWTAIIEAY ++G +++AIDLF  MR +G +PN+FTF+V LSIC EAGFVD+
Sbjct: 545 TIRVKGSMTWTAIIEAYRDNGLYEDAIDLFYEMRDKGFTPNNFTFQVALSICNEAGFVDD 604

Query: 641 ALRIFKLMSVRYKIKPSEEHYSLVIAILTRFGRLEEARRYVQMLSSLS 689
           A RIF LM+  Y +K SEE YSL+I +LTRFGR+E A+RY+Q+ SSLS
Sbjct: 605 ACRIFNLMTRSYNVKASEEQYSLIIGLLTRFGRVEAAQRYMQLSSSLS 647

BLAST of Cucsa.237230 vs. TrEMBL
Match: A0A061EEF5_THECC (Pentatricopeptide repeat (PPR-like) superfamily protein OS=Theobroma cacao GN=TCM_018189 PE=4 SV=1)

HSP 1 Score: 937.9 bits (2423), Expect = 6.8e-270
Identity = 456/689 (66.18%), Postives = 565/689 (82.00%), Query Frame = 1

Query: 1   MEISSSFIISLHLQPFTPNSLAPATAICNSGHRLSRIKSTTDTPPSKIKIVSKFRNRKRP 60
           ME + S  +S  L  F PN        C + ++ SRIK++  +PP   +  + F +R+ P
Sbjct: 1   MECNQSSSLSFCLHSFPPNPF-----FCRN-NQFSRIKASARSPPKPQRNPTIFAHRRSP 60

Query: 61  T-FAEKDAFPSSLPLHTKNPHAIYEDVQRFARQNKLKEALTIMDYVDQQGIPVNATTFSS 120
             F EK+AFPSSLPLHTKNPHAIY+D+QRFARQNKLKEAL I+DYVDQQGIPVN TTFSS
Sbjct: 61  PPFFEKNAFPSSLPLHTKNPHAIYKDIQRFARQNKLKEALAILDYVDQQGIPVNPTTFSS 120

Query: 121 LITACVRTKSMTYAKQIHAHIRINGLENNEFIRTRLVHMYTACGSLEEAQKLFDESSSKS 180
           L+ ACVR+KS+   +QIH+HIR NGLENNEF+R +L HMYT+CGS+++A ++FDE +SK+
Sbjct: 121 LLAACVRSKSLADGRQIHSHIRTNGLENNEFLRAKLAHMYTSCGSIDDALRVFDECTSKN 180

Query: 181 VYPWNALLRGTVMAGRRDYRSILSTYAEMRRLGVELNVYSFANIIKSFAGASAFTQGLKA 240
           V+ WNALLRGTV++G++ Y  +LSTY+EMR L V+LNVY+F+ ++KSFAGASAF QGLK 
Sbjct: 181 VHSWNALLRGTVISGKKRYLDVLSTYSEMRLLAVKLNVYTFSAVLKSFAGASAFRQGLKT 240

Query: 241 HGLLIKNGLIGSSLLGTTLVDMYFKCGKIKLARQMFGEITERDVVVWGSIIAGFAHNRLQ 300
           H LLIKNG I SS+L T L+D YFKCGKIKLA ++  EI ERD+V+WG++IAGFAHNR+Q
Sbjct: 241 HALLIKNGFIDSSMLRTGLIDFYFKCGKIKLACRVLEEIPERDIVLWGAMIAGFAHNRMQ 300

Query: 301 REALEYTRRMIDDGIRPNSVILTTILPVIGEIWARRLGQEVHAYVIKTKSYSKQIFIQSA 360
           +EAL Y R MI  GI PNSVILTTILPVIGE+WAR+LG+E+HAYV+KTKSYSKQ+ IQS 
Sbjct: 301 KEALSYVRWMISAGIYPNSVILTTILPVIGEVWARKLGREIHAYVVKTKSYSKQLVIQSG 360

Query: 361 LIDMYCKCGDIGSGRAVFYASMERNAICWTALMSGYALNGRLEQAVRSVIWMQQEGFRPD 420
           L+DMYCKCGD+ SGR VFY S ERNAI WTALMSGY  NGRL QA+RSV+WMQQEGF+PD
Sbjct: 361 LVDMYCKCGDMDSGRRVFYCSRERNAISWTALMSGYVSNGRLNQALRSVVWMQQEGFKPD 420

Query: 421 IVTVATILPVCAQLRALRPGKEIHAYAMKNCFLPNVSIVSSLMVMYSKCGVMDYTLKLFN 480
           +VTVATILPVCA+LRAL  GKEIHAYA+KNCF PNVSIV+SLM+MYSKCGV+DY+LKLFN
Sbjct: 421 VVTVATILPVCAELRALSHGKEIHAYAVKNCFFPNVSIVTSLMIMYSKCGVLDYSLKLFN 480

Query: 481 GMEQRNVILWTAMIDSYIENQCPHEAIDIFRAMQLSKHRPDTVTMSRILYICSEQKMLKM 540
           GME RNVI WTAMI+SY+++   HEA+ +FR+MQ SKHRPD+V M+R+L +CSE + +K+
Sbjct: 481 GMEARNVISWTAMIESYVKSGHLHEALSVFRSMQFSKHRPDSVAMARMLNVCSELRAVKL 540

Query: 541 GKEIHGQVLKRKFEPVHFVSAELVKLYGKCGAVKMAKMVFEAIPVKGPMTWTAIIEAYGE 600
           GKEIHGQVLK+ FE + FVSA +VK+YG CG +  AK+VFEA+PVKG MTWTAIIEAYG 
Sbjct: 541 GKEIHGQVLKKDFESIPFVSAGIVKMYGSCGLISTAKLVFEAVPVKGTMTWTAIIEAYGY 600

Query: 601 SGEFQEAIDLFDRMRSRGISPNHFTFKVVLSICKEAGFVDEALRIFKLMSVRYKIKPSEE 660
           +   ++AI LF +M S    PNHFTFKVVLS+C++AGFVD A ++F LM+ +Y++K SEE
Sbjct: 601 NDLCEDAISLFHQMASDDFIPNHFTFKVVLSVCRQAGFVDRACQLFSLMTRKYELKASEE 660

Query: 661 HYSLVIAILTRFGRLEEARRYVQMLSSLS 689
           HYS++I +L  FGR EEA R+VQM SSLS
Sbjct: 661 HYSIIIELLNTFGRFEEAERFVQM-SSLS 682

BLAST of Cucsa.237230 vs. TrEMBL
Match: F6HN33_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_18s0001g05930 PE=4 SV=1)

HSP 1 Score: 922.2 bits (2382), Expect = 3.8e-265
Identity = 446/616 (72.40%), Postives = 528/616 (85.71%), Query Frame = 1

Query: 71  SLPLHTKNPHAIYEDVQRFARQNKLKEALTIMDYVDQQGIPVNATTFSSLITACVRTKSM 130
           SLPLHTKNPHAI+ D+QRFARQ KLKEALTI+DY DQQGIPVN TTFSSL+ ACV +KS+
Sbjct: 2   SLPLHTKNPHAIFSDIQRFARQGKLKEALTILDYCDQQGIPVNPTTFSSLLRACVESKSL 61

Query: 131 TYAKQIHAHIRINGLENNEFIRTRLVHMYTACGSLEEAQKLFDESSSKSVYPWNALLRGT 190
           T+ KQIH HIRINGLENNEF+RT+LVHMYT+CGSLE+A+ +FD  SSKSVY WNALLRG 
Sbjct: 62  THGKQIHVHIRINGLENNEFLRTKLVHMYTSCGSLEDARGVFDGVSSKSVYTWNALLRGN 121

Query: 191 VMAGRRDYRSILSTYAEMRRLGVELNVYSFANIIKSFAGASAFTQGLKAHGLLIKNGLIG 250
           V++GRR YR  LSTY+EMR LGVELNVYSF+ +IKSFAGA+AF QGLKAH LLIKNGL+ 
Sbjct: 122 VISGRRHYREALSTYSEMRELGVELNVYSFSCMIKSFAGATAFRQGLKAHALLIKNGLVD 181

Query: 251 SSLLGTTLVDMYFKCGKIKLARQMFGEITERDVVVWGSIIAGFAHNRLQREALEYTRRMI 310
           SS+L T+L+DMYFKCGKIKLAR MF EI ERDVVVWG++IAGF HNRLQREALEY R M 
Sbjct: 182 SSILRTSLIDMYFKCGKIKLARLMFEEIVERDVVVWGAMIAGFGHNRLQREALEYLRWMR 241

Query: 311 DDGIRPNSVILTTILPVIGEIWARRLGQEVHAYVIKTKSYSKQIFIQSALIDMYCKCGDI 370
            +GI PNSVI+TTILPVIGE+ A +LG+EVHAYV+KTKSYSKQ+FIQSALIDMYCKCGD+
Sbjct: 242 REGICPNSVIMTTILPVIGEVGAWKLGREVHAYVVKTKSYSKQVFIQSALIDMYCKCGDM 301

Query: 371 GSGRAVFYASMERNAICWTALMSGYALNGRLEQAVRSVIWMQQEGFRPDIVTVATILPVC 430
            SGR VFYAS ERNA+ WTALMSGY  NGRL+QA+RS+ WMQQEGFRPD+VTVAT+LPVC
Sbjct: 302 ASGRQVFYASTERNAVSWTALMSGYVSNGRLDQALRSIAWMQQEGFRPDVVTVATVLPVC 361

Query: 431 AQLRALRPGKEIHAYAMKNCFLPNVSIVSSLMVMYSKCGVMDYTLKLFNGMEQRNVILWT 490
           A+LRALR GKEIH+YA+KN FLPNVSI +SLMVMYSKCG +DY+ KLF+GM+ RNVI WT
Sbjct: 362 AELRALRQGKEIHSYAVKNGFLPNVSIATSLMVMYSKCGNLDYSFKLFDGMDARNVISWT 421

Query: 491 AMIDSYIENQCPHEAIDIFRAMQLSKHRPDTVTMSRILYICSEQKMLKMGKEIHGQVLKR 550
           AMIDSY+EN C HEA+ +FR+MQLSKHRPD+V M+RIL IC E ++LK+GKEIHGQ+LK+
Sbjct: 422 AMIDSYVENGCLHEAVGVFRSMQLSKHRPDSVAMARILSICGELRVLKLGKEIHGQILKK 481

Query: 551 KFEPVHFVSAELVKLYGKCGAVKMAKMVFEAIPVKGPMTWTAIIEAYGESGEFQEAIDLF 610
            FE + FVSAE++K+YGK GA+  AK+ F+AIP KG M WTAIIEAYG +  +Q+AI+LF
Sbjct: 482 DFESIPFVSAEIIKMYGKFGAISKAKLAFKAIPAKGSMAWTAIIEAYGYNDLYQDAINLF 541

Query: 611 DRMRSRGISPNHFTFKVVLSICKEAGFVDEALRIFKLMSVRYKIKPSEEHYSLVIAILTR 670
            +M+S G  PNH+TFK VLSIC+ A   D+A  IF LMS RY+IK S EHYS +I +L R
Sbjct: 542 HQMQSDGFIPNHYTFKAVLSICERAELADDACLIFNLMSRRYRIKASNEHYSSIIELLNR 601

Query: 671 FGRLEEARRYVQMLSS 687
            GR E+A+R++QM S+
Sbjct: 602 VGRTEDAQRFIQMRSA 617

BLAST of Cucsa.237230 vs. TrEMBL
Match: A0A067LLI4_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_17232 PE=4 SV=1)

HSP 1 Score: 918.7 bits (2373), Expect = 4.2e-264
Identity = 455/690 (65.94%), Postives = 557/690 (80.72%), Query Frame = 1

Query: 1   MEISSSFIISLHLQPFTPNSLAPATAICNSGHRLSRIKSTTDTPPSKI--KIVSKFRNRK 60
           ME++ S  + ++L  F+PN   P     ++     +I +   TP  K   K + K++ +K
Sbjct: 1   MEVTLSRSLCINLHSFSPN---PFNNGDHNNKHFFKIMACMPTPYPKRYRKRIPKYQKKK 60

Query: 61  RPTFAEKDAFPSSLPLHTKNPHAIYEDVQRFARQNKLKEALTIMDYVDQQGIPVNATTFS 120
              F EKDAFP+SLPLH+KNP AI ED+Q+FAR+NKLKEALTIMDY+DQQGIPVN TTFS
Sbjct: 61  LKRFKEKDAFPASLPLHSKNPGAICEDIQKFARENKLKEALTIMDYLDQQGIPVNVTTFS 120

Query: 121 SLITACVRTKSMTYAKQIHAHIRINGLENNEFIRTRLVHMYTACGSLEEAQKLFDE--SS 180
           SLI AC+R+KS+  AKQIH  IRING ENNEF+RT+LVHMYTACGSL++AQ++FDE  SS
Sbjct: 121 SLIAACIRSKSLDQAKQIHVFIRINGFENNEFLRTKLVHMYTACGSLKDAQQVFDECSSS 180

Query: 181 SKSVYPWNALLRGTVMAGRRDYRSILSTYAEMRRLGVELNVYSFANIIKSFAGASAFTQG 240
           S SVYPWNALLRGTV++G + Y  +LSTY  MR LGVELNVYSF+N+IKSFAGASA  QG
Sbjct: 181 SSSVYPWNALLRGTVVSGSKRYLDVLSTYTTMRELGVELNVYSFSNVIKSFAGASALRQG 240

Query: 241 LKAHGLLIKNGLIGSSLLGTTLVDMYFKCGKIKLARQMFGEITERDVVVWGSIIAGFAHN 300
           LKAH +L+KNGLI SS+L T+L+DMYFKCGKIKLA ++F E  +RD+V WG++I+GFAHN
Sbjct: 241 LKAHAVLVKNGLIDSSILRTSLIDMYFKCGKIKLAHKVFEETLDRDIVFWGAMISGFAHN 300

Query: 301 RLQREALEYTRRMIDDGIRPNSVILTTILPVIGEIWARRLGQEVHAYVIKTKSYSKQIFI 360
           R Q EAL+Y R M+ +G+ PNSVI+TTIL VIG+ WAR+LG+E+H YV+KTKSYSKQ+ I
Sbjct: 301 RRQWEALDYFRWMVSEGMYPNSVIVTTILNVIGDKWARKLGKEIHGYVVKTKSYSKQLTI 360

Query: 361 QSALIDMYCKCGDIGSGRAVFYASMERNAICWTALMSGYALNGRLEQAVRSVIWMQQEGF 420
           QS LIDMYCKCGD+GS R VFY SMERNAI WTALMSGYA NGRLEQA+RSV WMQQEGF
Sbjct: 361 QSGLIDMYCKCGDMGSSRRVFYGSMERNAISWTALMSGYASNGRLEQALRSVSWMQQEGF 420

Query: 421 RPDIVTVATILPVCAQLRALRPGKEIHAYAMKNCFLPNVSIVSSLMVMYSKCGVMDYTLK 480
           RPD+VTVATI+PVC++L+AL  GKEIHAYA+KN F PNVS+ +SLM MYSKCGV+DY++K
Sbjct: 421 RPDVVTVATIVPVCSELKALNHGKEIHAYAVKNLFFPNVSVTTSLMKMYSKCGVLDYSVK 480

Query: 481 LFNGMEQRNVILWTAMIDSYIENQCPHEAIDIFRAMQLSKHRPDTVTMSRILYICSEQKM 540
           LFN ME RNVI WTA+IDSY EN C +EA+++FR+MQLSKHRPD+V MSR+L IC+E K 
Sbjct: 481 LFNNMESRNVISWTAIIDSYAENGCINEAMNVFRSMQLSKHRPDSVVMSRMLSICAEIKA 540

Query: 541 LKMGKEIHGQVLKRKFEPVHFVSAELVKLYGKCGAVKMAKMVFEAIPVKGPMTWTAIIEA 600
           +K+GKEIHG  +K+ FE + FVSA+LVK+YG+ G +  AK +F AIPVKG M WTAIIEA
Sbjct: 541 VKLGKEIHGHAIKKDFESIPFVSADLVKMYGRSGLIDNAKSIFHAIPVKGSMAWTAIIEA 600

Query: 601 YGESGEFQEAIDLFDRMRSRGISPNHFTFKVVLSICKEAGFVDEALRIFKLMSVRYKIKP 660
           YG +  +QEAI LF  M S G +P HFTFKVVLSIC +AGF D+A RIF+LMS RYKIK 
Sbjct: 601 YGYNNLWQEAIYLFHEMISGGFTPTHFTFKVVLSICDQAGFADDACRIFELMSRRYKIKA 660

Query: 661 SEEHYSLVIAILTRFGRLEEARRYVQMLSS 687
           SEEH S++  +LTR GR +EA R+ +M SS
Sbjct: 661 SEEHCSIIAGLLTRAGRTQEAERFTKMSSS 687

BLAST of Cucsa.237230 vs. TAIR10
Match: AT1G71460.1 (AT1G71460.1 Pentatricopeptide repeat (PPR-like) superfamily protein)

HSP 1 Score: 860.9 bits (2223), Expect = 5.3e-250
Identity = 417/674 (61.87%), Postives = 537/674 (79.67%), Query Frame = 1

Query: 18  PNSLAPATAICNSGHRLSRIKSTTDTP--PSKIKIVSKFRNRKRPTFAEKDAFPSSLPLH 77
           P SL+  T++ +  HR  +  +   +P  PS+ +  S    +K   F E+DAFPSSLPLH
Sbjct: 13  PASLSVTTSLNHRPHRSDKDGAPAKSPIRPSRTRRPSTSPAKKPKPFRERDAFPSSLPLH 72

Query: 78  TKNPHAIYEDVQRFARQNKLKEALTIMDYVDQQGIPVNATTFSSLITACVRTKSMTYAKQ 137
           +KNP+ I+ D+Q FARQN L+ ALTI+DY++Q+GIPVNATTFS+L+ ACVR KS+ + KQ
Sbjct: 73  SKNPYIIHRDIQIFARQNNLEVALTILDYLEQRGIPVNATTFSALLEACVRRKSLLHGKQ 132

Query: 138 IHAHIRINGLENNEFIRTRLVHMYTACGSLEEAQKLFDESSSKSVYPWNALLRGTVMAGR 197
           +H HIRINGLE+NEF+RT+LVHMYTACGS+++AQK+FDES+S +VY WNALLRGTV++G+
Sbjct: 133 VHVHIRINGLESNEFLRTKLVHMYTACGSVKDAQKVFDESTSSNVYSWNALLRGTVISGK 192

Query: 198 RDYRSILSTYAEMRRLGVELNVYSFANIIKSFAGASAFTQGLKAHGLLIKNGLIGSSLLG 257
           + Y+ +LST+ EMR LGV+LNVYS +N+ KSFAGASA  QGLK H L IKNGL  S  L 
Sbjct: 193 KRYQDVLSTFTEMRELGVDLNVYSLSNVFKSFAGASALRQGLKTHALAIKNGLFNSVFLK 252

Query: 258 TTLVDMYFKCGKIKLARQMFGEITERDVVVWGSIIAGFAHNRLQREALEYTRRMI-DDGI 317
           T+LVDMYFKCGK+ LAR++F EI ERD+VVWG++IAG AHN+ Q EAL   R MI ++ I
Sbjct: 253 TSLVDMYFKCGKVGLARRVFDEIVERDIVVWGAMIAGLAHNKRQWEALGLFRTMISEEKI 312

Query: 318 RPNSVILTTILPVIGEIWARRLGQEVHAYVIKTKSYSKQIFIQSALIDMYCKCGDIGSGR 377
            PNSVILTTILPV+G++ A +LG+EVHA+V+K+K+Y +Q F+ S LID+YCKCGD+ SGR
Sbjct: 313 YPNSVILTTILPVLGDVKALKLGKEVHAHVLKSKNYVEQPFVHSGLIDLYCKCGDMASGR 372

Query: 378 AVFYASMERNAICWTALMSGYALNGRLEQAVRSVIWMQQEGFRPDIVTVATILPVCAQLR 437
            VFY S +RNAI WTALMSGYA NGR +QA+RS++WMQQEGFRPD+VT+AT+LPVCA+LR
Sbjct: 373 RVFYGSKQRNAISWTALMSGYAANGRFDQALRSIVWMQQEGFRPDVVTIATVLPVCAELR 432

Query: 438 ALRPGKEIHAYAMKNCFLPNVSIVSSLMVMYSKCGVMDYTLKLFNGMEQRNVILWTAMID 497
           A++ GKEIH YA+KN FLPNVS+V+SLMVMYSKCGV +Y ++LF+ +EQRNV  WTAMID
Sbjct: 433 AIKQGKEIHCYALKNLFLPNVSLVTSLMVMYSKCGVPEYPIRLFDRLEQRNVKAWTAMID 492

Query: 498 SYIENQCPHEAIDIFRAMQLSKHRPDTVTMSRILYICSEQKMLKMGKEIHGQVLKRKFEP 557
            Y+EN      I++FR M LSKHRPD+VTM R+L +CS+ K LK+GKE+HG +LK++FE 
Sbjct: 493 CYVENCDLRAGIEVFRLMLLSKHRPDSVTMGRVLTVCSDLKALKLGKELHGHILKKEFES 552

Query: 558 VHFVSAELVKLYGKCGAVKMAKMVFEAIPVKGPMTWTAIIEAYGESGEFQEAIDLFDRMR 617
           + FVSA ++K+YGKCG ++ A   F+A+ VKG +TWTAIIEAYG +  F++AI+ F++M 
Sbjct: 553 IPFVSARIIKMYGKCGDLRSANFSFDAVAVKGSLTWTAIIEAYGCNELFRDAINCFEQMV 612

Query: 618 SRGISPNHFTFKVVLSICKEAGFVDEALRIFKLMSVRYKIKPSEEHYSLVIAILTRFGRL 677
           SRG +PN FTF  VLSIC +AGFVDEA R F LM   Y ++PSEEHYSLVI +L R GR+
Sbjct: 613 SRGFTPNTFTFTAVLSICSQAGFVDEAYRFFNLMLRMYNLQPSEEHYSLVIELLNRCGRV 672

Query: 678 EEARRYVQMLSSLS 689
           EEA+R   M SS S
Sbjct: 673 EEAQRLAVMSSSSS 686

BLAST of Cucsa.237230 vs. TAIR10
Match: AT4G18750.1 (AT4G18750.1 Pentatricopeptide repeat (PPR) superfamily protein)

HSP 1 Score: 349.0 bits (894), Expect = 6.8e-96
Identity = 192/598 (32.11%), Postives = 330/598 (55.18%), Query Frame = 1

Query: 86  VQRFARQNKLKEALTIMDYVDQQGIPVNATTFSSLITACVRTKSMTYAKQIHAHIRINGL 145
           ++RF     L+ A+ ++    +  I  +  T  S++  C  +KS+   K++   IR NG 
Sbjct: 68  LRRFCESGNLENAVKLLCVSGKWDI--DPRTLCSVLQLCADSKSLKDGKEVDNFIRGNGF 127

Query: 146 ENNEFIRTRLVHMYTACGSLEEAQKLFDESSSKSVYPWNALLRGTVMAGRRDYRSILSTY 205
             +  + ++L  MYT CG L+EA ++FDE   +    WN L+     +G  D+   +  +
Sbjct: 128 VIDSNLGSKLSLMYTNCGDLKEASRVFDEVKIEKALFWNILMNELAKSG--DFSGSIGLF 187

Query: 206 AEMRRLGVELNVYSFANIIKSFAGASAFTQGLKAHGLLIKNGLIGSSLLGTTLVDMYFKC 265
            +M   GVE++ Y+F+ + KSF+   +   G + HG ++K+G    + +G +LV  Y K 
Sbjct: 188 KKMMSSGVEMDSYTFSCVSKSFSSLRSVHGGEQLHGFILKSGFGERNSVGNSLVAFYLKN 247

Query: 266 GKIKLARQMFGEITERDVVVWGSIIAGFAHNRLQREALEYTRRMIDDGIRPNSVILTTIL 325
            ++  AR++F E+TERDV+ W SII G+  N L  + L    +M+  GI  +   + ++ 
Sbjct: 248 QRVDSARKVFDEMTERDVISWNSIINGYVSNGLAEKGLSVFVQMLVSGIEIDLATIVSVF 307

Query: 326 PVIGEIWARRLGQEVHAYVIKTKSYSKQIFIQSALIDMYCKCGDIGSGRAVFYASMERNA 385
               +     LG+ VH+  +K   +S++    + L+DMY KCGD+ S +AVF    +R+ 
Sbjct: 308 AGCADSRLISLGRAVHSIGVKA-CFSREDRFCNTLLDMYSKCGDLDSAKAVFREMSDRSV 367

Query: 386 ICWTALMSGYALNGRLEQAVRSVIWMQQEGFRPDIVTVATILPVCAQLRALRPGKEIHAY 445
           + +T++++GYA  G   +AV+    M++EG  PD+ TV  +L  CA+ R L  GK +H +
Sbjct: 368 VSYTSMIAGYAREGLAGEAVKLFEEMEEEGISPDVYTVTAVLNCCARYRLLDEGKRVHEW 427

Query: 446 AMKNCFLPNVSIVSSLMVMYSKCGVMDYTLKLFNGMEQRNVILWTAMIDSYIENQCPHEA 505
             +N    ++ + ++LM MY+KCG M     +F+ M  +++I W  +I  Y +N   +EA
Sbjct: 428 IKENDLGFDIFVSNALMDMYAKCGSMQEAELVFSEMRVKDIISWNTIIGGYSKNCYANEA 487

Query: 506 IDIFRAMQLSKH-RPDTVTMSRILYICSEQKMLKMGKEIHGQVLKRKFEPVHFVSAELVK 565
           + +F  +   K   PD  T++ +L  C+       G+EIHG +++  +     V+  LV 
Sbjct: 488 LSLFNLLLEEKRFSPDERTVACVLPACASLSAFDKGREIHGYIMRNGYFSDRHVANSLVD 547

Query: 566 LYGKCGAVKMAKMVFEAIPVKGPMTWTAIIEAYGESGEFQEAIDLFDRMRSRGISPNHFT 625
           +Y KCGA+ +A M+F+ I  K  ++WT +I  YG  G  +EAI LF++MR  GI  +  +
Sbjct: 548 MYAKCGALLLAHMLFDDIASKDLVSWTVMIAGYGMHGFGKEAIALFNQMRQAGIEADEIS 607

Query: 626 FKVVLSICKEAGFVDEALRIFKLMSVRYKIKPSEEHYSLVIAILTRFGRLEEARRYVQ 683
           F  +L  C  +G VDE  R F +M    KI+P+ EHY+ ++ +L R G L +A R+++
Sbjct: 608 FVSLLYACSHSGLVDEGWRFFNIMRHECKIEPTVEHYACIVDMLARTGDLIKAYRFIE 660

BLAST of Cucsa.237230 vs. TAIR10
Match: AT3G57430.1 (AT3G57430.1 Tetratricopeptide repeat (TPR)-like superfamily protein)

HSP 1 Score: 344.4 bits (882), Expect = 1.7e-94
Identity = 206/668 (30.84%), Postives = 346/668 (51.80%), Query Frame = 1

Query: 45  PSKIKIVSKFRN----RKRPTFAEKDAF------PSSLPLHTKNPHAIYEDVQRFARQNK 104
           PS++   S+ ++    R  PT A +D        PS     +++P    + ++   R N 
Sbjct: 18  PSQLLPFSRHKHPYLLRATPTSATEDVASAVSGAPSIFISQSRSPEWWIDLLRSKVRSNL 77

Query: 105 LKEALTIMDYVDQ--QGIPVNATTFSSLITACVRTKSMTYAKQIHAHIRINGLE-NNEFI 164
           L+EA  ++ YVD    GI  +   F +L+ A    + M   KQIHAH+   G   ++  +
Sbjct: 78  LREA--VLTYVDMIVLGIKPDNYAFPALLKAVADLQDMELGKQIHAHVYKFGYGVDSVTV 137

Query: 165 RTRLVHMYTACGSLEEAQKLFDESSSKSVYPWNALLRGTVMAGRRDYRSILSTYAEMRRL 224
              LV++Y  CG      K+FD  S ++   WN+L+    +     +   L  +  M   
Sbjct: 138 ANTLVNLYRKCGDFGAVYKVFDRISERNQVSWNSLISS--LCSFEKWEMALEAFRCMLDE 197

Query: 225 GVELNVYSFANIIKSFAGA---SAFTQGLKAHGLLIKNGLIGSSLLGTTLVDMYFKCGKI 284
            VE + ++  +++ + +          G + H   ++ G + S ++ T LV MY K GK+
Sbjct: 198 NVEPSSFTLVSVVTACSNLPMPEGLMMGKQVHAYGLRKGELNSFIINT-LVAMYGKLGKL 257

Query: 285 KLARQMFGEITERDVVVWGSIIAGFAHNRLQREALEYTRRMIDDGIRPNSVILTTILPVI 344
             ++ + G    RD+V W ++++    N    EALEY R M+ +G+ P+   ++++LP  
Sbjct: 258 ASSKVLLGSFGGRDLVTWNTVLSSLCQNEQLLEALEYLREMVLEGVEPDEFTISSVLPAC 317

Query: 345 GEIWARRLGQEVHAYVIKTKSYSKQIFIQSALIDMYCKCGDIGSGRAVFYASMERNAICW 404
             +   R G+E+HAY +K  S  +  F+ SAL+DMYC C  + SGR VF    +R    W
Sbjct: 318 SHLEMLRTGKELHAYALKNGSLDENSFVGSALVDMYCNCKQVLSGRRVFDGMFDRKIGLW 377

Query: 405 TALMSGYALNGRLEQAVRSVIWMQQE-GFRPDIVTVATILPVCAQLRALRPGKEIHAYAM 464
            A+++GY+ N   ++A+   I M++  G   +  T+A ++P C +  A    + IH + +
Sbjct: 378 NAMIAGYSQNEHDKEALLLFIGMEESAGLLANSTTMAGVVPACVRSGAFSRKEAIHGFVV 437

Query: 465 KNCFLPNVSIVSSLMVMYSKCGVMDYTLKLFNGMEQRNVILWTAMIDSYIENQCPHEAID 524
           K     +  + ++LM MYS+ G +D  +++F  ME R+++ W  MI  Y+ ++   +A+ 
Sbjct: 438 KRGLDRDRFVQNTLMDMYSRLGKIDIAMRIFGKMEDRDLVTWNTMITGYVFSEHHEDALL 497

Query: 525 IFRAMQ-----LSKH------RPDTVTMSRILYICSEQKMLKMGKEIHGQVLKRKFEPVH 584
           +   MQ     +SK       +P+++T+  IL  C+    L  GKEIH   +K       
Sbjct: 498 LLHKMQNLERKVSKGASRVSLKPNSITLMTILPSCAALSALAKGKEIHAYAIKNNLATDV 557

Query: 585 FVSAELVKLYGKCGAVKMAKMVFEAIPVKGPMTWTAIIEAYGESGEFQEAIDLFDRMRSR 644
            V + LV +Y KCG ++M++ VF+ IP K  +TW  II AYG  G  QEAIDL   M  +
Sbjct: 558 AVGSALVDMYAKCGCLQMSRKVFDQIPQKNVITWNVIIMAYGMHGNGQEAIDLLRMMMVQ 617

Query: 645 GISPNHFTFKVVLSICKEAGFVDEALRIFKLMSVRYKIKPSEEHYSLVIAILTRFGRLEE 685
           G+ PN  TF  V + C  +G VDE LRIF +M   Y ++PS +HY+ V+ +L R GR++E
Sbjct: 618 GVKPNEVTFISVFAACSHSGMVDEGLRIFYVMKPDYGVEPSSDHYACVVDLLGRAGRIKE 677


HSP 2 Score: 70.5 bits (171), Expect = 4.7e-12
Identity = 54/225 (24.00%), Postives = 105/225 (46.67%), Query Frame = 1

Query: 113 NATTFSSLITACVRTKSMTYAKQIHAHIRINGLENNEFIRTRLVHMYTACGSLEEAQKLF 172
           N+ T  +++ +C    ++   K+IHA+   N L  +  + + LV MY  CG L+ ++K+F
Sbjct: 516 NSITLMTILPSCAALSALAKGKEIHAYAIKNNLATDVAVGSALVDMYAKCGCLQMSRKVF 575

Query: 173 DESSSKSVYPWNALLRGTVMAGRRDYRSILSTYAEMRRLGVELNVYSFANIIKSFAGASA 232
           D+   K+V  WN ++    M G  + +  +     M   GV+ N  +F ++  + + +  
Sbjct: 576 DQIPQKNVITWNVIIMAYGMHG--NGQEAIDLLRMMMVQGVKPNEVTFISVFAACSHSGM 635

Query: 233 FTQGLKAHGLLIKN-GLIGSSLLGTTLVDMYFKCGKIKLARQMFGEITE--RDVVVWGSI 292
             +GL+   ++  + G+  SS     +VD+  + G+IK A Q+   +         W S+
Sbjct: 636 VDEGLRIFYVMKPDYGVEPSSDHYACVVDLLGRAGRIKEAYQLMNMMPRDFNKAGAWSSL 695

Query: 293 I-AGFAHNRLQ-REALEYTRRMIDDGIRPNSVILTTILPVIGEIW 333
           + A   HN L+  E        ++  +  + V+L  I    G +W
Sbjct: 696 LGASRIHNNLEIGEIAAQNLIQLEPNVASHYVLLANIYSSAG-LW 737

BLAST of Cucsa.237230 vs. TAIR10
Match: AT1G11290.1 (AT1G11290.1 Pentatricopeptide repeat (PPR) superfamily protein)

HSP 1 Score: 334.7 bits (857), Expect = 1.3e-91
Identity = 179/559 (32.02%), Postives = 307/559 (54.92%), Query Frame = 1

Query: 126 RTKSMTYAKQIHAHIRINGLENNEFIRTRLVHMYTACGSLEEAQKLFDESSSKSVYPWNA 185
           R  S+   +QI   +  NGL    F +T+LV ++   GS++EA ++F+   SK    ++ 
Sbjct: 46  RCSSLKELRQILPLVFKNGLYQEHFFQTKLVSLFCRYGSVDEAARVFEPIDSKLNVLYHT 105

Query: 186 LLRGTVMAGRRDYRSILSTYAEMRRLGVELNVYSFANIIKSFAGASAFTQGLKAHGLLIK 245
           +L+G   A   D    L  +  MR   VE  VY+F  ++K     +    G + HGLL+K
Sbjct: 106 MLKG--FAKVSDLDKALQFFVRMRYDDVEPVVYNFTYLLKVCGDEAELRVGKEIHGLLVK 165

Query: 246 NGLIGSSLLGTTLVDMYFKCGKIKLARQMFGEITERDVVVWGSIIAGFAHNRLQREALEY 305
           +G        T L +MY KC ++  AR++F  + ERD+V W +I+AG++ N + R ALE 
Sbjct: 166 SGFSLDLFAMTGLENMYAKCRQVNEARKVFDRMPERDLVSWNTIVAGYSQNGMARMALEM 225

Query: 306 TRRMIDDGIRPNSVILTTILPVIGEIWARRLGQEVHAYVIKTKSYSKQIFIQSALIDMYC 365
            + M ++ ++P+ + + ++LP +  +    +G+E+H Y +++  +   + I +AL+DMY 
Sbjct: 226 VKSMCEENLKPSFITIVSVLPAVSALRLISVGKEIHGYAMRS-GFDSLVNISTALVDMYA 285

Query: 366 KCGDIGSGRAVFYASMERNAICWTALMSGYALNGRLEQAVRSVIWMQQEGFRPDIVTVAT 425
           KCG + + R +F   +ERN + W +++  Y  N   ++A+     M  EG +P  V+V  
Sbjct: 286 KCGSLETARQLFDGMLERNVVSWNSMIDAYVQNENPKEAMLIFQKMLDEGVKPTDVSVMG 345

Query: 426 ILPVCAQLRALRPGKEIHAYAMKNCFLPNVSIVSSLMVMYSKCGVMDYTLKLFNGMEQRN 485
            L  CA L  L  G+ IH  +++     NVS+V+SL+ MY KC  +D    +F  ++ R 
Sbjct: 346 ALHACADLGDLERGRFIHKLSVELGLDRNVSVVNSLISMYCKCKEVDTAASMFGKLQSRT 405

Query: 486 VILWTAMIDSYIENQCPHEAIDIFRAMQLSKHRPDTVTMSRILYICSEQKMLKMGKEIHG 545
           ++ W AMI  + +N  P +A++ F  M+    +PDT T   ++   +E  +    K IHG
Sbjct: 406 LVSWNAMILGFAQNGRPIDALNYFSQMRSRTVKPDTFTYVSVITAIAELSITHHAKWIHG 465

Query: 546 QVLKRKFEPVHFVSAELVKLYGKCGAVKMAKMVFEAIPVKGPMTWTAIIEAYGESGEFQE 605
            V++   +   FV+  LV +Y KCGA+ +A+++F+ +  +   TW A+I+ YG  G  + 
Sbjct: 466 VVMRSCLDKNVFVTTALVDMYAKCGAIMIARLIFDMMSERHVTTWNAMIDGYGTHGFGKA 525

Query: 606 AIDLFDRMRSRGISPNHFTFKVVLSICKEAGFVDEALRIFKLMSVRYKIKPSEEHYSLVI 665
           A++LF+ M+   I PN  TF  V+S C  +G V+  L+ F +M   Y I+ S +HY  ++
Sbjct: 526 ALELFEEMQKGTIKPNGVTFLSVISACSHSGLVEAGLKCFYMMKENYSIELSMDHYGAMV 585

Query: 666 AILTRFGRLEEARRYVQML 685
            +L R GRL EA  ++  +
Sbjct: 586 DLLGRAGRLNEAWDFIMQM 601


HSP 2 Score: 224.6 bits (571), Expect = 1.9e-58
Identity = 136/519 (26.20%), Postives = 251/519 (48.36%), Query Frame = 1

Query: 73  PLHTKNPHAIYEDVQRFARQNKLKEALTI---MDYVDQQGIPVNATTFSSLITACVRTKS 132
           P+ +K     +  ++ FA+ + L +AL     M Y D + +  N   F+ L+  C     
Sbjct: 94  PIDSKLNVLYHTMLKGFAKVSDLDKALQFFVRMRYDDVEPVVYN---FTYLLKVCGDEAE 153

Query: 133 MTYAKQIHAHIRINGLENNEFIRTRLVHMYTACGSLEEAQKLFDESSSKSVYPWNALLRG 192
           +   K+IH  +  +G   + F  T L +MY  C  + EA+K+FD    + +  WN ++ G
Sbjct: 154 LRVGKEIHGLLVKSGFSLDLFAMTGLENMYAKCRQVNEARKVFDRMPERDLVSWNTIVAG 213

Query: 193 TVMAGRRDYRSILSTYAEMRRLGVELNVYSFANIIKSFAGASAFTQGLKAHGLLIKNGLI 252
               G    R  L     M    ++ +  +  +++ + +     + G + HG  +++G  
Sbjct: 214 YSQNGMA--RMALEMVKSMCEENLKPSFITIVSVLPAVSALRLISVGKEIHGYAMRSGFD 273

Query: 253 GSSLLGTTLVDMYFKCGKIKLARQMFGEITERDVVVWGSIIAGFAHNRLQREALEYTRRM 312
               + T LVDMY KCG ++ ARQ+F  + ER+VV W S+I  +  N   +EA+   ++M
Sbjct: 274 SLVNISTALVDMYAKCGSLETARQLFDGMLERNVVSWNSMIDAYVQNENPKEAMLIFQKM 333

Query: 313 IDDGIRPNSVILTTILPVIGEIWARRLGQEVHAYVIKTKSYSKQIFIQSALIDMYCKCGD 372
           +D+G++P  V +   L    ++     G+ +H   ++     + + + ++LI MYCKC +
Sbjct: 334 LDEGVKPTDVSVMGALHACADLGDLERGRFIHKLSVEL-GLDRNVSVVNSLISMYCKCKE 393

Query: 373 IGSGRAVFYASMERNAICWTALMSGYALNGRLEQAVRSVIWMQQEGFRPDIVTVATILPV 432
           + +  ++F     R  + W A++ G+A NGR   A+     M+    +PD  T  +++  
Sbjct: 394 VDTAASMFGKLQSRTLVSWNAMILGFAQNGRPIDALNYFSQMRSRTVKPDTFTYVSVITA 453

Query: 433 CAQLRALRPGKEIHAYAMKNCFLPNVSIVSSLMVMYSKCGVMDYTLKLFNGMEQRNVILW 492
            A+L      K IH   M++C   NV + ++L+ MY+KCG +     +F+ M +R+V  W
Sbjct: 454 IAELSITHHAKWIHGVVMRSCLDKNVFVTTALVDMYAKCGAIMIARLIFDMMSERHVTTW 513

Query: 493 TAMIDSYIENQCPHEAIDIFRAMQLSKHRPDTVTMSRILYICSEQKMLKMGKEIHGQVLK 552
            AMID Y  +     A+++F  MQ    +P+ VT   ++  CS   +++ G +    + +
Sbjct: 514 NAMIDGYGTHGFGKAALELFEEMQKGTIKPNGVTFLSVISACSHSGLVEAGLKCFYMMKE 573

Query: 553 R---KFEPVHFVSAELVKLYGKCGAVKMAKMVFEAIPVK 586
               +    H+    +V L G+ G +  A      +PVK
Sbjct: 574 NYSIELSMDHY--GAMVDLLGRAGRLNEAWDFIMQMPVK 604

BLAST of Cucsa.237230 vs. TAIR10
Match: AT4G21300.1 (AT4G21300.1 Tetratricopeptide repeat (TPR)-like superfamily protein)

HSP 1 Score: 324.7 bits (831), Expect = 1.4e-88
Identity = 180/600 (30.00%), Postives = 313/600 (52.17%), Query Frame = 1

Query: 86  VQRFARQNKLKEALTIMDYVDQQGIPVNATTFSSLITACVRTKSMTYAKQIHAHIRINGL 145
           +  F R   L +AL     +   G+  + +TF  L+ ACV  K+      +   +   G+
Sbjct: 110 ISSFVRNGLLNQALAFYFKMLCFGVSPDVSTFPCLVKACVALKNFKGIDFLSDTVSSLGM 169

Query: 146 ENNEFIRTRLVHMYTACGSLEEAQKLFDESSSKSVYPWNALLRGTVMAGRRDYRSILSTY 205
           + NEF+ + L+  Y   G ++   KLFD    K    WN +L G    G  D  S++  +
Sbjct: 170 DCNEFVASSLIKAYLEYGKIDVPSKLFDRVLQKDCVIWNVMLNGYAKCGALD--SVIKGF 229

Query: 206 AEMRRLGVELNVYSFANIIKSFAGASAFTQGLKAHGLLIKNGLIGSSLLGTTLVDMYFKC 265
           + MR   +  N  +F  ++   A       G++ HGL++ +G+     +  +L+ MY KC
Sbjct: 230 SVMRMDQISPNAVTFDCVLSVCASKLLIDLGVQLHGLVVVSGVDFEGSIKNSLLSMYSKC 289

Query: 266 GKIKLARQMFGEITERDVVVWGSIIAGFAHNRLQREALEYTRRMIDDGIRPNSVILTTIL 325
           G+   A ++F  ++  D V W  +I+G+  + L  E+L +   MI  G+ P+++  +++L
Sbjct: 290 GRFDDASKLFRMMSRADTVTWNCMISGYVQSGLMEESLTFFYEMISSGVLPDAITFSSLL 349

Query: 326 PVIGEIWARRLGQEVHAYVIKTKSYSKQIFIQSALIDMYCKCGDIGSGRAVFYASMERNA 385
           P + +       +++H Y+++  S S  IF+ SALID Y KC  +   + +F      + 
Sbjct: 350 PSVSKFENLEYCKQIHCYIMR-HSISLDIFLTSALIDAYFKCRGVSMAQNIFSQCNSVDV 409

Query: 386 ICWTALMSGYALNGRLEQAVRSVIWMQQEGFRPDIVTVATILPVCAQLRALRPGKEIHAY 445
           + +TA++SGY  NG    ++    W+ +    P+ +T+ +ILPV   L AL+ G+E+H +
Sbjct: 410 VVFTAMISGYLHNGLYIDSLEMFRWLVKVKISPNEITLVSILPVIGILLALKLGRELHGF 469

Query: 446 AMKNCFLPNVSIVSSLMVMYSKCGVMDYTLKLFNGMEQRNVILWTAMIDSYIENQCPHEA 505
            +K  F    +I  +++ MY+KCG M+   ++F  + +R+++ W +MI    ++  P  A
Sbjct: 470 IIKKGFDNRCNIGCAVIDMYAKCGRMNLAYEIFERLSKRDIVSWNSMITRCAQSDNPSAA 529

Query: 506 IDIFRAMQLSKHRPDTVTMSRILYICSEQKMLKMGKEIHGQVLKRKFEPVHFVSAELVKL 565
           IDIFR M +S    D V++S  L  C+       GK IHG ++K       +  + L+ +
Sbjct: 530 IDIFRQMGVSGICYDCVSISAALSACANLPSESFGKAIHGFMIKHSLASDVYSESTLIDM 589

Query: 566 YGKCGAVKMAKMVFEAIPVKGPMTWTAIIEAYGESGEFQEAIDLFDRM-RSRGISPNHFT 625
           Y KCG +K A  VF+ +  K  ++W +II A G  G+ ++++ LF  M    GI P+  T
Sbjct: 590 YAKCGNLKAAMNVFKTMKEKNIVSWNSIIAACGNHGKLKDSLCLFHEMVEKSGIRPDQIT 649

Query: 626 FKVVLSICKEAGFVDEALRIFKLMSVRYKIKPSEEHYSLVIAILTRFGRLEEARRYVQML 685
           F  ++S C   G VDE +R F+ M+  Y I+P +EHY+ V+ +  R GRL EA   V+ +
Sbjct: 650 FLEIISSCCHVGDVDEGVRFFRSMTEDYGIQPQQEHYACVVDLFGRAGRLTEAYETVKSM 706


HSP 2 Score: 224.6 bits (571), Expect = 1.9e-58
Identity = 131/517 (25.34%), Postives = 253/517 (48.94%), Query Frame = 1

Query: 118 SSLITACVRTKSMTYAKQIHAHIRINGLENNEFIRTRLVHMYTACGSLEEAQKLFDESSS 177
           S L+ AC     +   KQ+HA + +N +  + +   R++ MY  CGS  +  K+F     
Sbjct: 39  SLLLQACSNPNLLRQGKQVHAFLIVNSISGDSYTDERILGMYAMCGSFSDCGKMFYRLDL 98

Query: 178 K--SVYPWNALLRGTVMAGRRDYRSILSTYAEMRRLGVELNVYSFANIIKSFAGASAFTQ 237
           +  S+ PWN+++   V  G  +    L+ Y +M   GV  +V +F  ++K+      F  
Sbjct: 99  RRSSIRPWNSIISSFVRNGLLN--QALAFYFKMLCFGVSPDVSTFPCLVKACVALKNFKG 158

Query: 238 GLKAHGLLIKNGLIGSSLLGTTLVDMYFKCGKIKLARQMFGEITERDVVVWGSIIAGFAH 297
                  +   G+  +  + ++L+  Y + GKI +  ++F  + ++D V+W  ++ G+A 
Sbjct: 159 IDFLSDTVSSLGMDCNEFVASSLIKAYLEYGKIDVPSKLFDRVLQKDCVIWNVMLNGYAK 218

Query: 298 NRLQREALEYTRRMIDDGIRPNSVILTTILPVIGEIWARRLGQEVHAYVIKTKSYSKQIF 357
                  ++    M  D I PN+V    +L V        LG ++H  V+       +  
Sbjct: 219 CGALDSVIKGFSVMRMDQISPNAVTFDCVLSVCASKLLIDLGVQLHGLVV-VSGVDFEGS 278

Query: 358 IQSALIDMYCKCGDIGSGRAVFYASMERNAICWTALMSGYALNGRLEQAVRSVIWMQQEG 417
           I+++L+ MY KCG       +F      + + W  ++SGY  +G +E+++     M   G
Sbjct: 279 IKNSLLSMYSKCGRFDDASKLFRMMSRADTVTWNCMISGYVQSGLMEESLTFFYEMISSG 338

Query: 418 FRPDIVTVATILPVCAQLRALRPGKEIHAYAMKNCFLPNVSIVSSLMVMYSKCGVMDYTL 477
             PD +T +++LP  ++   L   K+IH Y M++    ++ + S+L+  Y KC  +    
Sbjct: 339 VLPDAITFSSLLPSVSKFENLEYCKQIHCYIMRHSISLDIFLTSALIDAYFKCRGVSMAQ 398

Query: 478 KLFNGMEQRNVILWTAMIDSYIENQCPHEAIDIFRAMQLSKHRPDTVTMSRILYICSEQK 537
            +F+     +V+++TAMI  Y+ N    +++++FR +   K  P+ +T+  IL +     
Sbjct: 399 NIFSQCNSVDVVVFTAMISGYLHNGLYIDSLEMFRWLVKVKISPNEITLVSILPVIGILL 458

Query: 538 MLKMGKEIHGQVLKRKFEPVHFVSAELVKLYGKCGAVKMAKMVFEAIPVKGPMTWTAIIE 597
            LK+G+E+HG ++K+ F+    +   ++ +Y KCG + +A  +FE +  +  ++W ++I 
Sbjct: 459 ALKLGRELHGFIIKKGFDNRCNIGCAVIDMYAKCGRMNLAYEIFERLSKRDIVSWNSMIT 518

Query: 598 AYGESGEFQEAIDLFDRMRSRGISPNHFTFKVVLSIC 633
              +S     AID+F +M   GI  +  +    LS C
Sbjct: 519 RCAQSDNPSAAIDIFRQMGVSGICYDCVSISAALSAC 552


HSP 3 Score: 95.1 bits (235), Expect = 1.8e-19
Identity = 64/265 (24.15%), Postives = 121/265 (45.66%), Query Frame = 1

Query: 423 VATILPVCAQLRALRPGKEIHAYAMKNCFLPNVSIVSSLMVMYSKCGVMDYTLKLFNGME 482
           ++ +L  C+    LR GK++HA+ + N    +      ++ MY+ CG      K+F  ++
Sbjct: 38  LSLLLQACSNPNLLRQGKQVHAFLIVNSISGDSYTDERILGMYAMCGSFSDCGKMFYRLD 97

Query: 483 QRNVIL--WTAMIDSYIENQCPHEAIDIFRAMQLSKHRPDTVTMSRILYICSEQKMLKMG 542
            R   +  W ++I S++ N   ++A+  +  M      PD  T   ++  C   K  K  
Sbjct: 98  LRRSSIRPWNSIISSFVRNGLLNQALAFYFKMLCFGVSPDVSTFPCLVKACVALKNFKGI 157

Query: 543 KEIHGQVLKRKFEPVHFVSAELVKLYGKCGAVKMAKMVFEAIPVKGPMTWTAIIEAYGES 602
             +   V     +   FV++ L+K Y + G + +   +F+ +  K  + W  ++  Y + 
Sbjct: 158 DFLSDTVSSLGMDCNEFVASSLIKAYLEYGKIDVPSKLFDRVLQKDCVIWNVMLNGYAKC 217

Query: 603 GEFQEAIDLFDRMRSRGISPNHFTFKVVLSICKEAGFVDEALRIFKLMSVRYKIKPSEEH 662
           G     I  F  MR   ISPN  TF  VLS+C     +D  +++  L+ V          
Sbjct: 218 GALDSVIKGFSVMRMDQISPNAVTFDCVLSVCASKLLIDLGVQLHGLVVVSGVDFEGSIK 277

Query: 663 YSLVIAILTRFGRLEEARRYVQMLS 686
            SL +++ ++ GR ++A +  +M+S
Sbjct: 278 NSL-LSMYSKCGRFDDASKLFRMMS 301

BLAST of Cucsa.237230 vs. NCBI nr
Match: gi|778707902|ref|XP_011656084.1| (PREDICTED: pentatricopeptide repeat-containing protein At1g71460, chloroplastic [Cucumis sativus])

HSP 1 Score: 1365.1 bits (3532), Expect = 0.0e+00
Identity = 688/688 (100.00%), Postives = 688/688 (100.00%), Query Frame = 1

Query: 1   MEISSSFIISLHLQPFTPNSLAPATAICNSGHRLSRIKSTTDTPPSKIKIVSKFRNRKRP 60
           MEISSSFIISLHLQPFTPNSLAPATAICNSGHRLSRIKSTTDTPPSKIKIVSKFRNRKRP
Sbjct: 1   MEISSSFIISLHLQPFTPNSLAPATAICNSGHRLSRIKSTTDTPPSKIKIVSKFRNRKRP 60

Query: 61  TFAEKDAFPSSLPLHTKNPHAIYEDVQRFARQNKLKEALTIMDYVDQQGIPVNATTFSSL 120
           TFAEKDAFPSSLPLHTKNPHAIYEDVQRFARQNKLKEALTIMDYVDQQGIPVNATTFSSL
Sbjct: 61  TFAEKDAFPSSLPLHTKNPHAIYEDVQRFARQNKLKEALTIMDYVDQQGIPVNATTFSSL 120

Query: 121 ITACVRTKSMTYAKQIHAHIRINGLENNEFIRTRLVHMYTACGSLEEAQKLFDESSSKSV 180
           ITACVRTKSMTYAKQIHAHIRINGLENNEFIRTRLVHMYTACGSLEEAQKLFDESSSKSV
Sbjct: 121 ITACVRTKSMTYAKQIHAHIRINGLENNEFIRTRLVHMYTACGSLEEAQKLFDESSSKSV 180

Query: 181 YPWNALLRGTVMAGRRDYRSILSTYAEMRRLGVELNVYSFANIIKSFAGASAFTQGLKAH 240
           YPWNALLRGTVMAGRRDYRSILSTYAEMRRLGVELNVYSFANIIKSFAGASAFTQGLKAH
Sbjct: 181 YPWNALLRGTVMAGRRDYRSILSTYAEMRRLGVELNVYSFANIIKSFAGASAFTQGLKAH 240

Query: 241 GLLIKNGLIGSSLLGTTLVDMYFKCGKIKLARQMFGEITERDVVVWGSIIAGFAHNRLQR 300
           GLLIKNGLIGSSLLGTTLVDMYFKCGKIKLARQMFGEITERDVVVWGSIIAGFAHNRLQR
Sbjct: 241 GLLIKNGLIGSSLLGTTLVDMYFKCGKIKLARQMFGEITERDVVVWGSIIAGFAHNRLQR 300

Query: 301 EALEYTRRMIDDGIRPNSVILTTILPVIGEIWARRLGQEVHAYVIKTKSYSKQIFIQSAL 360
           EALEYTRRMIDDGIRPNSVILTTILPVIGEIWARRLGQEVHAYVIKTKSYSKQIFIQSAL
Sbjct: 301 EALEYTRRMIDDGIRPNSVILTTILPVIGEIWARRLGQEVHAYVIKTKSYSKQIFIQSAL 360

Query: 361 IDMYCKCGDIGSGRAVFYASMERNAICWTALMSGYALNGRLEQAVRSVIWMQQEGFRPDI 420
           IDMYCKCGDIGSGRAVFYASMERNAICWTALMSGYALNGRLEQAVRSVIWMQQEGFRPDI
Sbjct: 361 IDMYCKCGDIGSGRAVFYASMERNAICWTALMSGYALNGRLEQAVRSVIWMQQEGFRPDI 420

Query: 421 VTVATILPVCAQLRALRPGKEIHAYAMKNCFLPNVSIVSSLMVMYSKCGVMDYTLKLFNG 480
           VTVATILPVCAQLRALRPGKEIHAYAMKNCFLPNVSIVSSLMVMYSKCGVMDYTLKLFNG
Sbjct: 421 VTVATILPVCAQLRALRPGKEIHAYAMKNCFLPNVSIVSSLMVMYSKCGVMDYTLKLFNG 480

Query: 481 MEQRNVILWTAMIDSYIENQCPHEAIDIFRAMQLSKHRPDTVTMSRILYICSEQKMLKMG 540
           MEQRNVILWTAMIDSYIENQCPHEAIDIFRAMQLSKHRPDTVTMSRILYICSEQKMLKMG
Sbjct: 481 MEQRNVILWTAMIDSYIENQCPHEAIDIFRAMQLSKHRPDTVTMSRILYICSEQKMLKMG 540

Query: 541 KEIHGQVLKRKFEPVHFVSAELVKLYGKCGAVKMAKMVFEAIPVKGPMTWTAIIEAYGES 600
           KEIHGQVLKRKFEPVHFVSAELVKLYGKCGAVKMAKMVFEAIPVKGPMTWTAIIEAYGES
Sbjct: 541 KEIHGQVLKRKFEPVHFVSAELVKLYGKCGAVKMAKMVFEAIPVKGPMTWTAIIEAYGES 600

Query: 601 GEFQEAIDLFDRMRSRGISPNHFTFKVVLSICKEAGFVDEALRIFKLMSVRYKIKPSEEH 660
           GEFQEAIDLFDRMRSRGISPNHFTFKVVLSICKEAGFVDEALRIFKLMSVRYKIKPSEEH
Sbjct: 601 GEFQEAIDLFDRMRSRGISPNHFTFKVVLSICKEAGFVDEALRIFKLMSVRYKIKPSEEH 660

Query: 661 YSLVIAILTRFGRLEEARRYVQMLSSLS 689
           YSLVIAILTRFGRLEEARRYVQMLSSLS
Sbjct: 661 YSLVIAILTRFGRLEEARRYVQMLSSLS 688

BLAST of Cucsa.237230 vs. NCBI nr
Match: gi|659119310|ref|XP_008459588.1| (PREDICTED: pentatricopeptide repeat-containing protein At1g71460, chloroplastic [Cucumis melo])

HSP 1 Score: 1309.3 bits (3387), Expect = 0.0e+00
Identity = 655/688 (95.20%), Postives = 674/688 (97.97%), Query Frame = 1

Query: 1   MEISSSFIISLHLQPFTPNSLAPATAICNSGHRLSRIKSTTDTPPSKIKIVSKFRNRKRP 60
           MEISSSF+ISLHLQPF PNSL  A+AICN GH+LSRIKSTTD PP KIKIVSKFRNRKRP
Sbjct: 1   MEISSSFLISLHLQPFPPNSLTAASAICNPGHQLSRIKSTTDIPPPKIKIVSKFRNRKRP 60

Query: 61  TFAEKDAFPSSLPLHTKNPHAIYEDVQRFARQNKLKEALTIMDYVDQQGIPVNATTFSSL 120
           TFAEKDAFPSSLPLHTKNPHAIYED+QRFARQNKLKEALTI+DYVDQQGIPVNATTFSSL
Sbjct: 61  TFAEKDAFPSSLPLHTKNPHAIYEDIQRFARQNKLKEALTILDYVDQQGIPVNATTFSSL 120

Query: 121 ITACVRTKSMTYAKQIHAHIRINGLENNEFIRTRLVHMYTACGSLEEAQKLFDESSSKSV 180
           ITACVRTKSMT AKQIHAHIRINGLENNEFIRTRLVHMYTACGSLE+AQKLFDESSSKSV
Sbjct: 121 ITACVRTKSMTDAKQIHAHIRINGLENNEFIRTRLVHMYTACGSLEDAQKLFDESSSKSV 180

Query: 181 YPWNALLRGTVMAGRRDYRSILSTYAEMRRLGVELNVYSFANIIKSFAGASAFTQGLKAH 240
           YPWNALLRGTVMAGRRDYRSILSTYAEMRRLGVELNVYSFANIIKSFAGASAFTQGLKAH
Sbjct: 181 YPWNALLRGTVMAGRRDYRSILSTYAEMRRLGVELNVYSFANIIKSFAGASAFTQGLKAH 240

Query: 241 GLLIKNGLIGSSLLGTTLVDMYFKCGKIKLARQMFGEITERDVVVWGSIIAGFAHNRLQR 300
            LLIKNGLIGSSLLGTTLVDMYFKCGKIKLARQMF EITERDVVVWGSIIAGFAHNRLQR
Sbjct: 241 SLLIKNGLIGSSLLGTTLVDMYFKCGKIKLARQMFEEITERDVVVWGSIIAGFAHNRLQR 300

Query: 301 EALEYTRRMIDDGIRPNSVILTTILPVIGEIWARRLGQEVHAYVIKTKSYSKQIFIQSAL 360
           EAL YTRRMIDDGIRPNSVILTTILPVIGEIWARRLGQEVHAYVIKTKSYSKQIFIQS+L
Sbjct: 301 EALVYTRRMIDDGIRPNSVILTTILPVIGEIWARRLGQEVHAYVIKTKSYSKQIFIQSSL 360

Query: 361 IDMYCKCGDIGSGRAVFYASMERNAICWTALMSGYALNGRLEQAVRSVIWMQQEGFRPDI 420
           IDMYCKCGDIGSGRAVFYASMERNAICWTALMSGYALNGRLEQAVRSVIWMQQEGFRPD+
Sbjct: 361 IDMYCKCGDIGSGRAVFYASMERNAICWTALMSGYALNGRLEQAVRSVIWMQQEGFRPDV 420

Query: 421 VTVATILPVCAQLRALRPGKEIHAYAMKNCFLPNVSIVSSLMVMYSKCGVMDYTLKLFNG 480
           VTVATILPVCAQLRALRPGKEIHAYA+KNCFLPNVSIVSSLMVMYSKCGV+DY+LKLFNG
Sbjct: 421 VTVATILPVCAQLRALRPGKEIHAYAVKNCFLPNVSIVSSLMVMYSKCGVIDYSLKLFNG 480

Query: 481 MEQRNVILWTAMIDSYIENQCPHEAIDIFRAMQLSKHRPDTVTMSRILYICSEQKMLKMG 540
           MEQRNVILWTAMIDSY+ENQCPHEAIDIFRAMQLSKHRPDTVTM+RILY+CSE K+LKMG
Sbjct: 481 MEQRNVILWTAMIDSYVENQCPHEAIDIFRAMQLSKHRPDTVTMARILYVCSELKVLKMG 540

Query: 541 KEIHGQVLKRKFEPVHFVSAELVKLYGKCGAVKMAKMVFEAIPVKGPMTWTAIIEAYGES 600
           KEIHGQVLKRKFE VHFVS+ELVKLYGKCGAVKMAKMVFEAIPVKGPMTWTAIIEAYGE+
Sbjct: 541 KEIHGQVLKRKFEQVHFVSSELVKLYGKCGAVKMAKMVFEAIPVKGPMTWTAIIEAYGEN 600

Query: 601 GEFQEAIDLFDRMRSRGISPNHFTFKVVLSICKEAGFVDEALRIFKLMSVRYKIKPSEEH 660
           GEFQEAIDLFDRMRS GISPNHFTFKVVLSICKEAGFVDEALRIFKLMSVRYKIKPSEEH
Sbjct: 601 GEFQEAIDLFDRMRSCGISPNHFTFKVVLSICKEAGFVDEALRIFKLMSVRYKIKPSEEH 660

Query: 661 YSLVIAILTRFGRLEEARRYVQMLSSLS 689
           YSLVIA+LTRFGR+EEARRYVQM SSLS
Sbjct: 661 YSLVIAVLTRFGRMEEARRYVQMSSSLS 688

BLAST of Cucsa.237230 vs. NCBI nr
Match: gi|1009138392|ref|XP_015886564.1| (PREDICTED: pentatricopeptide repeat-containing protein At1g71460, chloroplastic [Ziziphus jujuba])

HSP 1 Score: 966.1 bits (2496), Expect = 3.3e-278
Identity = 473/702 (67.38%), Postives = 577/702 (82.19%), Query Frame = 1

Query: 1   MEISSSFIISLHLQPFTPNSLA--------------PATAICNSGHRLSRIKSTTDTPPS 60
           ME  S   +SLH   F  NS A              PA+AI  +    ++ +    +P S
Sbjct: 1   MEAVSCSTMSLHA--FPSNSFASTPINGNYPTFKPKPASAIVGTRAPSTKHRRKKPSPKS 60

Query: 61  KIKIVSKFRNRKRPTFAEKDAFPSSLPLHTKNPHAIYEDVQRFARQNKLKEALTIMDYVD 120
           K          K  TF EKDAFP SLPLH+KNPHAIYED+QRFARQNKL +  TI+DY++
Sbjct: 61  K----------KPRTFTEKDAFPESLPLHSKNPHAIYEDIQRFARQNKLDQVFTILDYLN 120

Query: 121 QQGIPVNATTFSSLITACVRTKSMTYAKQIHAHIRINGLENNEFIRTRLVHMYTACGSLE 180
           QQGIPVN TTFSS+I ACVRTKS+   K+IHAHIR+NGLE+NEF+RT+LVHMYT+CG+ E
Sbjct: 121 QQGIPVNPTTFSSIIAACVRTKSLAQGKKIHAHIRMNGLESNEFLRTKLVHMYTSCGAFE 180

Query: 181 EAQKLFDESSSKSVYPWNALLRGTVMAGRRDYRSILSTYAEMRRLGVELNVYSFANIIKS 240
           +AQ +F E SS SVYPWNALLRG+V+AG R YR +LSTY+EMR LG+ELNVY+F+N+IKS
Sbjct: 181 DAQMVFGECSSGSVYPWNALLRGSVIAGGRRYRDVLSTYSEMRALGIELNVYTFSNVIKS 240

Query: 241 FAGASAFTQGLKAHGLLIKNGLIGSSLLGTTLVDMYFKCGKIKLARQMFGEITERDVVVW 300
           FAGASAF QGLK H LLIKNGLI S++L T+L+DMYFKCGKIKLAR++F EI ERD+VVW
Sbjct: 241 FAGASAFAQGLKTHALLIKNGLIDSAILRTSLIDMYFKCGKIKLARRVFEEIQERDIVVW 300

Query: 301 GSIIAGFAHNRLQREALEYTRRMIDDGIRPNSVILTTILPVIGEIWARRLGQEVHAYVIK 360
           G +IAGFAHNRL  EALEY RRMID+G+RPNSVILTTILP+IGE+W+ +LG+EVHA V+K
Sbjct: 301 GVMIAGFAHNRLHTEALEYVRRMIDEGLRPNSVILTTILPIIGEVWSHKLGREVHACVLK 360

Query: 361 TKSYSKQIFIQSALIDMYCKCGDIGSGRAVFYASMERNAICWTALMSGYALNGRLEQAVR 420
           TKSYSKQIFIQSALIDMYCKCGD+ SGR VFY S ERNAICWTAL+SGY  NGRLEQA+R
Sbjct: 361 TKSYSKQIFIQSALIDMYCKCGDMESGRRVFYRSKERNAICWTALISGYVTNGRLEQALR 420

Query: 421 SVIWMQQEGFRPDIVTVATILPVCAQLRALRPGKEIHAYAMKNCFLPNVSIVSSLMVMYS 480
           S+IWMQQEGF PD+VTVAT+LPVCA+LRAL+ GKEIHAY++KNCFLPNVS+VSSLM+MYS
Sbjct: 421 SIIWMQQEGFVPDVVTVATVLPVCAELRALKRGKEIHAYSVKNCFLPNVSVVSSLMIMYS 480

Query: 481 KCGVMDYTLKLFNGMEQRNVILWTAMIDSYIENQCPHEAIDIFRAMQLSKHRPDTVTMSR 540
           KCG+++Y++KLF G+E+RNVILWTAMIDSY++N C HEA+ + R+M LSKHRPDTV M+R
Sbjct: 481 KCGILNYSIKLFEGLEKRNVILWTAMIDSYVDNGCLHEALSVIRSMLLSKHRPDTVAMAR 540

Query: 541 ILYICSEQKMLKMGKEIHGQVLKRKFEPVHFVSAELVKLYGKCGAVKMAKMVFEAIPVKG 600
           +L +C E K LK+GKEIHGQ LK+ F+ VHFVSAE++K+YG CG + +A +VFE IP+KG
Sbjct: 541 MLSVCCELKNLKLGKEIHGQALKKDFDSVHFVSAEIMKMYGSCGVLDIANLVFENIPIKG 600

Query: 601 PMTWTAIIEAYGESGEFQEAIDLFDRMRSRGISPNHFTFKVVLSICKEAGFVDEALRIFK 660
            MTWTAIIEAY  +G +Q+AIDLFD+M S+G +PN FTF+VVLSIC +AG VDEA +IF 
Sbjct: 601 SMTWTAIIEAYRYNGLYQDAIDLFDQMASKGFTPNPFTFEVVLSICDQAGLVDEACQIFN 660

Query: 661 LMSVRYKIKPSEEHYSLVIAILTRFGRLEEARRYVQMLSSLS 689
           LMS +YKIK S+E Y+++I +LTRFGR +EA+R++QM SSLS
Sbjct: 661 LMSRKYKIKASKEQYTMMIGLLTRFGRFDEAQRFIQM-SSLS 689

BLAST of Cucsa.237230 vs. NCBI nr
Match: gi|703062664|ref|XP_010086776.1| (hypothetical protein L484_001633 [Morus notabilis])

HSP 1 Score: 951.8 bits (2459), Expect = 6.5e-274
Identity = 454/648 (70.06%), Postives = 552/648 (85.19%), Query Frame = 1

Query: 41  TDTPPSKIKIVSKFRNRKRPTFAEKDAFPSSLPLHTKNPHAIYEDVQRFARQNKLKEALT 100
           T TP S  K     R RKRP F +KDAFP SLPLH+KNP A+Y D+QRFARQNKL +ALT
Sbjct: 5   TRTPASPGK-----RRRKRPVFTKKDAFPESLPLHSKNPRAVYSDIQRFARQNKLSQALT 64

Query: 101 IMDYVDQQGIPVNATTFSSLITACVRTKSMTYAKQIHAHIRINGLENNEFIRTRLVHMYT 160
           I+DY+DQQGIPVN TTF++LI ACVRTKS+ + KQ+HA IRINGL+ NEF+RT+LVHMYT
Sbjct: 65  ILDYMDQQGIPVNPTTFAALIAACVRTKSLDHGKQVHAFIRINGLDKNEFLRTKLVHMYT 124

Query: 161 ACGSLEEAQKLFDESSSKSVYPWNALLRGTVMAGRRDYRSILSTYAEMRRLGVELNVYSF 220
           +CGS+++A  LFDES S+SVYPWNALLRG V++G R YR  LSTY +MR LG+E+NVYSF
Sbjct: 125 SCGSVDDANNLFDESPSRSVYPWNALLRGNVISGGRRYRDALSTYYQMRALGIEMNVYSF 184

Query: 221 ANIIKSFAGASAFTQGLKAHGLLIKNGLIGSSLLGTTLVDMYFKCGKIKLARQMFGEITE 280
           +++IKS AGASA  QGLK H LLIKNGL+GS++L T+L+DMYFKCGKIKLARQ+F EI E
Sbjct: 185 SSVIKSLAGASALLQGLKTHALLIKNGLVGSAMLRTSLIDMYFKCGKIKLARQVFEEIVE 244

Query: 281 RDVVVWGSIIAGFAHNRLQREALEYTRRMIDDGIRPNSVILTTILPVIGEIWARRLGQEV 340
           RD+V WG++I+GFAHNRLQ +AL+YTRRM+D+GI+ NSVILT ILPVIGE+ AR+LG+EV
Sbjct: 245 RDIVAWGAMISGFAHNRLQWQALDYTRRMVDEGIKLNSVILTIILPVIGELLARKLGREV 304

Query: 341 HAYVIKTKSYSKQIFIQSALIDMYCKCGDIGSGRAVFYASMERNAICWTALMSGYALNGR 400
           HAY +KTK Y+KQ FIQS LIDMYCKCGD+ +GR VFY   ERNAICWTAL+SGY  NGR
Sbjct: 305 HAYAVKTKRYAKQTFIQSGLIDMYCKCGDMENGRRVFYRLKERNAICWTALISGYVANGR 364

Query: 401 LEQAVRSVIWMQQEGFRPDIVTVATILPVCAQLRALRPGKEIHAYAMKNCFLPNVSIVSS 460
           LEQA+RS+IWMQQEG RPD+VTVAT++P+CA+LRAL+PGKEIHAYA+KNCFLPNVSIVSS
Sbjct: 365 LEQALRSIIWMQQEGIRPDVVTVATVVPICAELRALKPGKEIHAYAVKNCFLPNVSIVSS 424

Query: 461 LMVMYSKCGVMDYTLKLFNGMEQRNVILWTAMIDSYIENQCPHEAIDIFRAMQLSKHRPD 520
           LM+MYSKCGV+DY+++LF GMEQRNVILWTAMIDSY+EN+   EA+ + R+M LSKHRPD
Sbjct: 425 LMMMYSKCGVLDYSVRLFEGMEQRNVILWTAMIDSYVENRHLDEALSVIRSMVLSKHRPD 484

Query: 521 TVTMSRILYICSEQKMLKMGKEIHGQVLKRKFEPVHFVSAELVKLYGKCGAVKMAKMVFE 580
           +V + R+L IC+E K LK GKEIHGQVLKR FE VHFVSAE+VK+YG+CG +  AK+VF+
Sbjct: 485 SVAIGRMLCICNELKSLKFGKEIHGQVLKRNFESVHFVSAEIVKMYGRCGVIDDAKLVFD 544

Query: 581 AIPVKGPMTWTAIIEAYGESGEFQEAIDLFDRMRSRGISPNHFTFKVVLSICKEAGFVDE 640
            I VKG MTWTAIIEAY ++G +++AIDLF  MR +G +PN+FTF+V LSIC EAGFVD+
Sbjct: 545 TIRVKGSMTWTAIIEAYRDNGLYEDAIDLFYEMRDKGFTPNNFTFQVALSICNEAGFVDD 604

Query: 641 ALRIFKLMSVRYKIKPSEEHYSLVIAILTRFGRLEEARRYVQMLSSLS 689
           A RIF LM+  Y +K SEE YSL+I +LTRFGR+E A+RY+Q+ SSLS
Sbjct: 605 ACRIFNLMTRSYNVKASEEQYSLIIGLLTRFGRVEAAQRYMQLSSSLS 647

BLAST of Cucsa.237230 vs. NCBI nr
Match: gi|645253041|ref|XP_008232399.1| (PREDICTED: pentatricopeptide repeat-containing protein At1g71460, chloroplastic [Prunus mume])

HSP 1 Score: 951.4 bits (2458), Expect = 8.5e-274
Identity = 460/641 (71.76%), Postives = 554/641 (86.43%), Query Frame = 1

Query: 47  KIKIVSKFRNRKRPTFAEKDAFPSSLPLHTKNPHAIYEDVQRFARQNKLKEALTIMDYVD 106
           K+K +++ R +K PTFAE DAFP SLPLHTKNPHAIY+D+Q FAR+NKLKEALTI+DY+D
Sbjct: 43  KLKALTR-RQQKTPTFAENDAFPDSLPLHTKNPHAIYKDIQSFARRNKLKEALTILDYLD 102

Query: 107 QQGIPVNATTFSSLITACVRTKSMTYAKQIHAHIRINGLENNEFIRTRLVHMYTACGSLE 166
           QQGIPVNATTFSSLI ACVRT+S  + KQIH HIRINGLE+N+FIRT+LVHMYT+ GS+E
Sbjct: 103 QQGIPVNATTFSSLIAACVRTRSEDHGKQIHTHIRINGLESNDFIRTKLVHMYTSFGSVE 162

Query: 167 EAQKLFDESSSKSVYPWNALLRGTVMAGRRDYRSILSTYAEMRRLGVELNVYSFANIIKS 226
           +AQ+LFDESS+KSVY WNALLRGTV++G R YR +L TY EMR LGVELNVYSF++++KS
Sbjct: 163 DAQQLFDESSTKSVYSWNALLRGTVISGGRRYRDVLHTYTEMRALGVELNVYSFSSVMKS 222

Query: 227 FAGASAFTQGLKAHGLLIKNGLIGSSLLGTTLVDMYFKCGKIKLARQMFGEITERDVVVW 286
           FAGASA +QGLK H LL+KNG I SS++ T+LVD+YFKCGKI+LA ++F E  ERDVVVW
Sbjct: 223 FAGASALSQGLKTHALLVKNGFIDSSIVRTSLVDLYFKCGKIRLAHRVFEEFGERDVVVW 282

Query: 287 GSIIAGFAHNRLQREALEYTRRMIDDGIRPNSVILTTILPVIGEIWARRLGQEVHAYVIK 346
           G++IAGFAHNR QREALEY R M+D+GIRPNSVILT+ILPVIG++ AR+LGQEVHA+V+K
Sbjct: 283 GTMIAGFAHNRRQREALEYARMMVDEGIRPNSVILTSILPVIGDVGARKLGQEVHAFVLK 342

Query: 347 TKSYSKQIFIQSALIDMYCKCGDIGSGRAVFYASMERNAICWTALMSGYALNGRLEQAVR 406
           TKSYSKQIFIQS LIDMYCKCGD+  GR VFY S ERNAICWTALMSGY  NGR EQA+R
Sbjct: 343 TKSYSKQIFIQSGLIDMYCKCGDMDMGRRVFYHSKERNAICWTALMSGYVANGRPEQALR 402

Query: 407 SVIWMQQEGFRPDIVTVATILPVCAQLRALRPGKEIHAYAMKNCFLPNVSIVSSLMVMYS 466
           SVIWMQQEGF+PD+VTVAT+LPVCA+L+ L+ GKEIHAYA+KNCFLPNVSI+SSLMVMYS
Sbjct: 403 SVIWMQQEGFKPDLVTVATVLPVCAELKDLKRGKEIHAYAVKNCFLPNVSIISSLMVMYS 462

Query: 467 KCGVMDYTLKLFNGMEQRNVILWTAMIDSYIENQCPHEAIDIFRAMQLSKHRPDTVTMSR 526
           KCG+  Y+ +LF+GMEQRNVILWTAMIDSYI+N C +EA+ + R+M LSKHRPD+V  +R
Sbjct: 463 KCGIFKYSRRLFDGMEQRNVILWTAMIDSYIDNGCLYEALGVIRSMLLSKHRPDSVATAR 522

Query: 527 ILYICSEQKMLKMGKEIHGQVLKRKFEPVHFVSAELVKLYGKCGAVKMAKMVFEAIPVKG 586
           IL  C+  K LK+GKEIHGQVLK+ FE + FV++E+VK+YG CG V  AK  F  IPVKG
Sbjct: 523 ILTTCNGLKNLKLGKEIHGQVLKKDFESIPFVASEIVKMYGHCGEVDHAKSAFNIIPVKG 582

Query: 587 PMTWTAIIEAYGESGEFQEAIDLFDRMRSRGISPNHFTFKVVLSICKEAGFVDEALRIFK 646
            MTWTAIIEAY  +G +++AIDLFD MRS+  +PNHFTF+VVLSIC +AGFV++A RIF 
Sbjct: 583 SMTWTAIIEAYAYNGMYRDAIDLFDEMRSKDFTPNHFTFQVVLSICDQAGFVNDACRIFH 642

Query: 647 LMSVRYKIKPSEEHYSLVIAILTRFGRLEEARRYVQMLSSL 688
           LMS  YK+K SEE YSL+I +LTRFGR++EA+R++Q+ SSL
Sbjct: 643 LMSRVYKVKVSEEQYSLIIGLLTRFGRVKEAQRFLQLSSSL 682

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PP115_ARATH9.4e-24961.87Pentatricopeptide repeat-containing protein At1g71460, chloroplastic OS=Arabidop... [more]
PP320_ARATH1.2e-9432.11Pentatricopeptide repeat-containing protein DOT4, chloroplastic OS=Arabidopsis t... [more]
PP285_ARATH3.0e-9330.84Pentatricopeptide repeat-containing protein At3g57430, chloroplastic OS=Arabidop... [more]
PPR32_ARATH2.3e-9032.02Pentatricopeptide repeat-containing protein At1g11290, chloroplastic OS=Arabidop... [more]
PP333_ARATH2.4e-8730.00Pentatricopeptide repeat-containing protein At4g21300 OS=Arabidopsis thaliana GN... [more]
Match NameE-valueIdentityDescription
A0A0A0KXW0_CUCSA0.0e+00100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_5G649310 PE=4 SV=1[more]
W9T1A9_9ROSA4.5e-27470.06Uncharacterized protein OS=Morus notabilis GN=L484_001633 PE=4 SV=1[more]
A0A061EEF5_THECC6.8e-27066.18Pentatricopeptide repeat (PPR-like) superfamily protein OS=Theobroma cacao GN=TC... [more]
F6HN33_VITVI3.8e-26572.40Putative uncharacterized protein OS=Vitis vinifera GN=VIT_18s0001g05930 PE=4 SV=... [more]
A0A067LLI4_JATCU4.2e-26465.94Uncharacterized protein OS=Jatropha curcas GN=JCGZ_17232 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G71460.15.3e-25061.87 Pentatricopeptide repeat (PPR-like) superfamily protein[more]
AT4G18750.16.8e-9632.11 Pentatricopeptide repeat (PPR) superfamily protein[more]
AT3G57430.11.7e-9430.84 Tetratricopeptide repeat (TPR)-like superfamily protein[more]
AT1G11290.11.3e-9132.02 Pentatricopeptide repeat (PPR) superfamily protein[more]
AT4G21300.11.4e-8830.00 Tetratricopeptide repeat (TPR)-like superfamily protein[more]
Match NameE-valueIdentityDescription
gi|778707902|ref|XP_011656084.1|0.0e+00100.00PREDICTED: pentatricopeptide repeat-containing protein At1g71460, chloroplastic ... [more]
gi|659119310|ref|XP_008459588.1|0.0e+0095.20PREDICTED: pentatricopeptide repeat-containing protein At1g71460, chloroplastic ... [more]
gi|1009138392|ref|XP_015886564.1|3.3e-27867.38PREDICTED: pentatricopeptide repeat-containing protein At1g71460, chloroplastic ... [more]
gi|703062664|ref|XP_010086776.1|6.5e-27470.06hypothetical protein L484_001633 [Morus notabilis][more]
gi|645253041|ref|XP_008232399.1|8.5e-27471.76PREDICTED: pentatricopeptide repeat-containing protein At1g71460, chloroplastic ... [more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR002885Pentatricopeptide_repeat
IPR011990TPR-like_helical_dom_sf
Vocabulary: Molecular Function
TermDefinition
GO:0005515protein binding
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008152 metabolic process
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0008568 microtubule-severing ATPase activity
molecular_function GO:0005515 protein binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.237230.1Cucsa.237230.1mRNA


Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002885Pentatricopeptide repeatPFAMPF01535PPRcoord: 284..314
score: 0.14coord: 155..175
score: 0.93coord: 459..486
score: 0.9coord: 488..513
score: 0.0015coord: 256..282
score: 0
IPR002885Pentatricopeptide repeatPFAMPF13041PPR_2coord: 384..430
score: 1.9E-7coord: 588..632
score: 3.4
IPR002885Pentatricopeptide repeatPFAMPF13812PPR_3coord: 101..146
score: 0.
IPR002885Pentatricopeptide repeatTIGRFAMsTIGR00756TIGR00756coord: 488..521
score: 5.6E-6coord: 589..621
score: 2.2E-10coord: 623..657
score: 0
IPR002885Pentatricopeptide repeatPROFILEPS51375PPRcoord: 78..112
score: 5.579coord: 282..316
score: 10.402coord: 485..519
score: 9.767coord: 621..656
score: 7.947coord: 657..688
score: 6.939coord: 216..250
score: 5.766coord: 183..215
score: 5.196coord: 251..281
score: 6.686coord: 454..484
score: 7.004coord: 586..620
score: 13.121coord: 520..554
score: 7.574coord: 148..182
score: 8.572coord: 384..418
score: 10.742coord: 113..147
score: 7.794coord: 353..383
score: 6.029coord: 419..453
score: 5
IPR011990Tetratricopeptide-like helical domainGENE3DG3DSA:1.25.40.10coord: 589..622
score: 2.5E-5coord: 260..313
score: 2.5E-5coord: 483..535
score: 2.
NoneNo IPR availablePANTHERPTHR24015FAMILY NOT NAMEDcoord: 134..419
score: 2.0E-279coord: 455..684
score: 2.0E
NoneNo IPR availablePANTHERPTHR24015:SF755SUBFAMILY NOT NAMEDcoord: 455..684
score: 2.0E-279coord: 134..419
score: 2.0E