Cucsa.235740 (gene) Cucumber (Gy14) v1

NameCucsa.235740
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionAnaphase-promoting complex subunit 4-like protein
Locationscaffold02012 : 438730 .. 443153 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTAAAATTGCCGAGTGGAACCCTGAAAAGGATTTGCTAGCCATGGTCACGGAAGACTCGAAAATTTTGCTCCACCGTTTCAACTGGCAGAGGCTGTGGACTATATCGCCTGGTGAGTTCTATGTTTCCTTTGTTTCTGTAGCTTATCTTTTACATTTCCTTATTTCTTAAAAGAAAATTCAACGGGTGTGTTCTAGTTTTCATTTTAACACTGCTTTATTGTCTTTGTTCGATCTAAAGAAGTGTTTCTAATATACGTTGAAGTTTTTTTTTGTTAGAGTGTAATGCATAGGTTAGTTAAATTGATATTAAGCTTTTGATACTAATTCATGACGAAGGGCAATATTTCATCTGCCAAAAGACTATCTTTATGTTATTTAATGATAACTTAGAGATTCAACTTTTGGTGTTACACTAAAGTTTAAATTCTTGCTTTGGACAATTTTAGTTCAACAAAACTTAATACATCAACATCAAAACCATATTGTCACTTCCTCTTAAGACCTCTGTGTTTTTCACTTTGTTATTGGGTTCTTACTTGTTATGTTGTTTGGTGACATCCTTGGCTAGGAATGATTGTATAACTTTTATGTAGGAAGGTCTATAAAATCGTTATGTTGGAGGCCTGATGGTAAGGTGATAGCAGTTGGACTTGAAGATGGGACAGTTTTATTGCATGATGTTGAAGTGAGTAGTCTTACTTTAACTTTAAAACACAGTTTTGCTATTGATTAGTCAATTGTGGAACTCTTGCACTCAATTATGGTGATCCAGACTCCTGCTAGTCCAGTTGTACTATAATTATTTAGATTCAGCTTCCAATATGCCTAATATAGATTTAGTTCTCTTGCTGCTTTTTAGACCCTTTATTTTGTACGTCTAACAGAATGGAAAGTTGTTGAGAAGCCTAAAGTCTCATGCAGTTGCTGTGGTTAGTCTAAATTGGGTGGAGGATAGCCAGTTGATTACAGTAAGTAATTCTCCCCTTATTTATTTGGTGTTTTCCATGAAGTTTATTGGAAAGTCATTTTGCAAGCCTCATCTAGAATGTGCAGCATGAATAATGATTTTCAAATATGGGTTTATGTTTTTATCCAAAAATAAAATTGTGTTTCCCTCTCATCCTTCACTCATTCTCAAAATTGTGGTTCAGGACAAAAATGAAATCTTGTCAACTTATGAAGATCGCACTCGCCGTATTTTCCCTCCTGCTCCTACAATTCCTCGAATGCCTGGACTTGTCTCTGGAGATACTGGTTTCATTGACGACTCTGAAGACTCATTTACGGAGTTGTCAAATTCTTCTCAACAGCGGTTTAACATCTTGTGCAGTGGTGACAAAGATGGAAGCATCCGTTTCAGCATATTTGGCGTTTTTCCAATTGGAAAAATAGTAAGTTAACTGCGATTATATTTATAACCACAACTGCAAACCTTTCTTCTCTCTCTTCAAAAAATAGGTACTTAACTGATTTTTTTCTCGCTTCATCAGTATAGTTCTTGAATACATTGATTCACTTGTATGAAATTCAATGTAGTATGTTAATTTCAAAATTGTATACTTCTATTTTGATTGTGATCGCTTCTTGCACACTCAATGCATAAAGACCAAAACTCGACCCCTAGCTTTCCTATCCTCTGCTGGCCTTTTGGTGAATACAGACCAGCAGAGGACGAAAAAGCTAGGGTTCCGGATTTGGTCTTATATTTTCAAGTGATCAGGAAGTGATGTCAATATACAGTTTTGAAATTAAAATACTACTTTTAAATTGTATTACAAGAGAAACAAAATAATTTTATCAGTGTATTTGAGAACTATACTGATGAATGGAGAGAAGTTAGTAGTTGAGTAATGATTTTCCAAAGAGAGATAAGAAAGGTTCGTGGTTATAGTTATGTATAATTTCTTAATCCTAAAATGATCTGTTTGATAAAGGTTTTCTGTTCTATAAAACTATTTTTCTTGTACACTCCCTTTTTGAGTATTTGGAATGCAAAGCATCTGCTTTCTTTTTTTCTTTATCATTTAGACATTGACATTAGAAGGTAACTGTGATAAAAAAACCACGTGCAGTGTAGTTATACAATGTGTAACTGGAGATTTATGTTTTTTCCCCCTTTTAACAGATGCAAGTTTATTTTTATTGTTTTTTGTGTTGTTACCGTCACTGTATTGATGTATTGAGTGTCTCATCCATTGATGTGCTTTTCTTTTTCAATGGGACAGAACATACACGAGCTTCATATTCCTCTTCAAGATGCTAGTGCTTCATGTCACCTTCTGAATGCTGAAATTTACAAGGTATGATATAACAGCTATTCTACATTTATGGACTTTATTTGATCATATGGCCTGTTCCTTTTGGGCAATCATTTTTAATTTGTTATTCATAGCTGTGGAAATATGGTGACTTCGAGCAACAGGAGTATGGTGAAATTTAAATGGAGTTTAACTTATAATTTTTATTACTTATATTGGTTCAAGTGAAGAGTTTTAATTGGTCCATATCTTGGCATGAATACGTTACTTACTTCTAAATCAACTTGTAACCTTTGAATATTTGAAGTTTCAAGCAGAAGCTTGTGGAAGTGAGATTTCTTATGCATATGGAGCTCGAGCTTCAAGTTTTAAAAATCTCCTCCAAACTATTAAAGCATCGTGGTTGTTAAATTAAATAATTTAATATCAAGGATTATCTGTTCGGTCTTTAAGATTTCTAGTTAGATTAATATCTTACTTTAATATAAAAATTTCTCCAATAATTTTCACTGTACCTGAAATGTTTGTCTGAACTGGGAGTATTGTAACTTGGTTTCAAGTTTCAGAAATCTTGATTGGTTTATGAGTAAATACGATAATGTACATAATTTGTTTTGTATTAGTGTAGGTTGCTTTATCGAAGGATTTTTGTCGTTTGGTAGTAATGTGCTCAGGCGAACTCGTTGGACATGGGCATGATCCAAGAAAAAGACAAATCACCGTTCAAGGTGTGCATGGCATGCATTCTTTAGTGCTTGATACTTCAATCTTTAGGAAGAGGTAATTACTGCTGACACTAGGAATATTACTGAAATATTTGTCCTTGGATTTAGATTTTCAGGTTTTGTACTTCTATCTCTTGAATTCTGATAGGAATATGGTAGGAGCCAAAAGGGGATTGTAGATATTATTAAGGGCATATTAGTAATTACATTGTTAATTGTTGTAGTTGAATGGTCATAAGTAGGATTTAGGGGTAGGAAGAGAAGCAGATGTTGGGAATTCTCAATATTCCCTTGAAATTTTCTTTGGAATTAAAATAATTTCCACTCCTCCCCCACGATTTCCAGACTCTGGTATAGGTTACCATTGGATTCTCATTATGCTTCTTTCTAGTTACCCTAATATATCTCTAAGAAAAAGCTGGTATGATATTCAAGCATTTTTTTCTTGTTGAAAGGTTGGTTGAGTCCTACTGAATTATATCTTATCTAGTATCGTCCCATCATATCCTTTCTAACCTTTGTGCTCCTGGAAAAAGAAAAGAAAAACTCATTTTCTGCCTCATTGTAGGAAAAGTGAGCTGCATCAGGTAGCTCAACAAGCTTCTAACATTGGGGAATTGACTGAGGTAATAAGGGTATCATTATCAGTAATGAGTAAGCAGTGGTCTGATGCCATGCACGTTTTCCAAGAGAAGTTTGACTCTTTATCCACGCTGATAGTTAACCATGGTAGTAATAGATATTATCGATATTCATGTGCTACTCCTTTTACCCTGACTCACGTCATTTCTTTTCCTACATATAGGACTTGACTCATCTGCCCAGGAGGAGTTTCTTAGCATTCTAGGTGGTGCACGAACTAGTCCTCCAGTTCACCAATTTTTAGTTAACTCTCTTGGTGAAGTGGTACGCACAGTCTTCTCTTTGATCATTTCAGATATTAATGATTGTTTTGTTGTTCACTCCGATTTTTTGAATAGGGTGCAAAGCGTGTGTCTAAGGCTATTTCTGGGGCTGGAAGTGAACTCCAACTTATTGTTCTAGATCATCTTCAGGTGAAAATGCATACAGTAATATTTTATTATTATTCTTTACTATTATAGGTTTTTTTTTTTTGACATGATATCAATACAGCTATATACTAGTAGAACTTAAATGGTAGAATTTCTTTGCAGCCTGCTGCAGAAATCATTGGATTTCGAATGGGCGAACTATTAGGGATTTCTAGATGGCGTGCACGTTTTCAGGGTGTTGGTTTGGATGAGAAGCTTATGCACAATGCTACAGAAAAGGTTGGTACGCTACTGGTGCAAGTTGAACGGTTTATGAGGGTGCTTTCAACAGTCTTGCAGCAGGTATGCCCTATGGAAGATGAATTTTTGCTATGGTTTTCATTTGAACGCAAATTTTGCTGTGTACCAAAGCCCCCTCCCCAATAA

mRNA sequence

GTTAAAATTGCCGAGTGGAACCCTGAAAAGGATTTGCTAGCCATGGTCACGGAAGACTCGAAAATTTTGCTCCACCGTTTCAACTGGCAGAGGCTGTGGACTATATCGCCTGGAAGGTCTATAAAATCGTTATGTTGGAGGCCTGATGGTAAGGTGATAGCAGTTGGACTTGAAGATGGGACAGTTTTATTGCATGATGTTGAAAATGGAAAGTTGTTGAGAAGCCTAAAGTCTCATGCAGTTGCTGTGGTTAGTCTAAATTGGGTGGAGGATAGCCAGTTGATTACAGACAAAAATGAAATCTTGTCAACTTATGAAGATCGCACTCGCCGTATTTTCCCTCCTGCTCCTACAATTCCTCGAATGCCTGGACTTGTCTCTGGAGATACTGGTTTCATTGACGACTCTGAAGACTCATTTACGGAGTTGTCAAATTCTTCTCAACAGCGGTTTAACATCTTGTGCAGTGGTGACAAAGATGGAAGCATCCGTTTCAGCATATTTGGCGTTTTTCCAATTGGAAAAATAGTTGCTTTATCGAAGGATTTTTGTCGTTTGGTAGTAATGTGCTCAGGCGAACTCGTTGGACATGGGCATGATCCAAGAAAAAGACAAATCACCGTTCAAGGTGTGCATGGCATGCATTCTTTAGTGCTTGATACTTCAATCTTTAGGAAGAGGAAAAGTGAGCTGCATCAGGTAGCTCAACAAGCTTCTAACATTGGGGAATTGACTGAGGTAATAAGGGTATCATTATCAGTAATGAGTAAGCAGTGGTCTGATGCCATGCACGTTTTCCAAGAGAAGTTTGACTCTTTATCCACGCTGATAGTTAACCATGGACTTGACTCATCTGCCCAGGAGGAGTTTCTTAGCATTCTAGGTGGTGCACGAACTAGTCCTCCAGTTCACCAATTTTTAGTTAACTCTCTTGGTGAAGTGGGTGCAAAGCGTGTGTCTAAGGCTATTTCTGGGGCTGGAAGTGAACTCCAACTTATTGTTCTAGATCATCTTCAGCCTGCTGCAGAAATCATTGGATTTCGAATGGGCGAACTATTAGGGATTTCTAGATGGCGTGCACGTTTTCAGGGTGTTGGTTTGGATGAGAAGCTTATGCACAATGCTACAGAAAAGGTTGGTACGCTACTGGTGCAAGTTGAACGGTTTATGAGGGTGCTTTCAACAGTCTTGCAGCAGGTATGCCCTATGGAAGATGAATTTTTGCTATGGTTTTCATTTGAACGCAAATTTTGCTGTGTACCAAAGCCCCCTCCCCAATAA

Coding sequence (CDS)

GTTAAAATTGCCGAGTGGAACCCTGAAAAGGATTTGCTAGCCATGGTCACGGAAGACTCGAAAATTTTGCTCCACCGTTTCAACTGGCAGAGGCTGTGGACTATATCGCCTGGAAGGTCTATAAAATCGTTATGTTGGAGGCCTGATGGTAAGGTGATAGCAGTTGGACTTGAAGATGGGACAGTTTTATTGCATGATGTTGAAAATGGAAAGTTGTTGAGAAGCCTAAAGTCTCATGCAGTTGCTGTGGTTAGTCTAAATTGGGTGGAGGATAGCCAGTTGATTACAGACAAAAATGAAATCTTGTCAACTTATGAAGATCGCACTCGCCGTATTTTCCCTCCTGCTCCTACAATTCCTCGAATGCCTGGACTTGTCTCTGGAGATACTGGTTTCATTGACGACTCTGAAGACTCATTTACGGAGTTGTCAAATTCTTCTCAACAGCGGTTTAACATCTTGTGCAGTGGTGACAAAGATGGAAGCATCCGTTTCAGCATATTTGGCGTTTTTCCAATTGGAAAAATAGTTGCTTTATCGAAGGATTTTTGTCGTTTGGTAGTAATGTGCTCAGGCGAACTCGTTGGACATGGGCATGATCCAAGAAAAAGACAAATCACCGTTCAAGGTGTGCATGGCATGCATTCTTTAGTGCTTGATACTTCAATCTTTAGGAAGAGGAAAAGTGAGCTGCATCAGGTAGCTCAACAAGCTTCTAACATTGGGGAATTGACTGAGGTAATAAGGGTATCATTATCAGTAATGAGTAAGCAGTGGTCTGATGCCATGCACGTTTTCCAAGAGAAGTTTGACTCTTTATCCACGCTGATAGTTAACCATGGACTTGACTCATCTGCCCAGGAGGAGTTTCTTAGCATTCTAGGTGGTGCACGAACTAGTCCTCCAGTTCACCAATTTTTAGTTAACTCTCTTGGTGAAGTGGGTGCAAAGCGTGTGTCTAAGGCTATTTCTGGGGCTGGAAGTGAACTCCAACTTATTGTTCTAGATCATCTTCAGCCTGCTGCAGAAATCATTGGATTTCGAATGGGCGAACTATTAGGGATTTCTAGATGGCGTGCACGTTTTCAGGGTGTTGGTTTGGATGAGAAGCTTATGCACAATGCTACAGAAAAGGTTGGTACGCTACTGGTGCAAGTTGAACGGTTTATGAGGGTGCTTTCAACAGTCTTGCAGCAGGTATGCCCTATGGAAGATGAATTTTTGCTATGGTTTTCATTTGAACGCAAATTTTGCTGTGTACCAAAGCCCCCTCCCCAATAA

Protein sequence

VKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISPGRSIKSLCWRPDGKVIAVGLEDGTVLLHDVENGKLLRSLKSHAVAVVSLNWVEDSQLITDKNEILSTYEDRTRRIFPPAPTIPRMPGLVSGDTGFIDDSEDSFTELSNSSQQRFNILCSGDKDGSIRFSIFGVFPIGKIVALSKDFCRLVVMCSGELVGHGHDPRKRQITVQGVHGMHSLVLDTSIFRKRKSELHQVAQQASNIGELTEVIRVSLSVMSKQWSDAMHVFQEKFDSLSTLIVNHGLDSSAQEEFLSILGGARTSPPVHQFLVNSLGEVGAKRVSKAISGAGSELQLIVLDHLQPAAEIIGFRMGELLGISRWRARFQGVGLDEKLMHNATEKVGTLLVQVERFMRVLSTVLQQVCPMEDEFLLWFSFERKFCCVPKPPPQ*
BLAST of Cucsa.235740 vs. Swiss-Prot
Match: APC4_ARATH (Anaphase-promoting complex subunit 4 OS=Arabidopsis thaliana GN=APC4 PE=2 SV=2)

HSP 1 Score: 572.4 bits (1474), Expect = 4.1e-162
Identity = 290/426 (68.08%), Postives = 336/426 (78.87%), Query Frame = 1

Query: 1   VKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISPGRSIKSLCWRPDGKVIAVGLEDG 60
           +KIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISPG+ + SLCWRPDGK IAVGLEDG
Sbjct: 27  IKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISPGKPVTSLCWRPDGKAIAVGLEDG 86

Query: 61  TVLLHDVENGKLLRSLKSHAVAVVSLNWVEDSQLITDKNEILSTYEDRTRRIFPPAPTIP 120
           T+ LHDVENGKLLR+LK H VAVV LNW ED Q  TD++   S YEDRT R FPPAP  P
Sbjct: 87  TISLHDVENGKLLRNLKPHDVAVVCLNWEEDGQSNTDESGNFSVYEDRTSRFFPPAPRPP 146

Query: 121 RMPGLVSGDTGFIDDSEDSFTELSNSSQQRFNILCSGDKDGSIRFSIFGVFPIGKI---- 180
           +MPGLV+GD+ F+DD EDS  ELSN+S ++FNILC+GD+DG+I FSIFG+F IGKI    
Sbjct: 147 KMPGLVAGDSSFMDDGEDSLAELSNTSFRKFNILCTGDRDGNICFSIFGIFQIGKINIHE 206

Query: 181 -----------------------VALSKDFCRLVVMCSGELVGHGHDPRKRQITVQGVHG 240
                                  VALSKD CRLVVMC+GEL      PR+ +I VQ + G
Sbjct: 207 LSLPVPHLDEHASCKLFNASIYKVALSKDLCRLVVMCTGELKDCDIKPREEKINVQDLPG 266

Query: 241 MHSLVLDTSIFRKRKSELHQVAQQASNIGELTEVIRVSLSVMSKQWSDAMHVFQEKFDSL 300
           +H L +DTSIF KRK ELHQVAQQASNI +LTEVIR SLSVM+KQW+DAM  F EKF SL
Sbjct: 267 LHCLAMDTSIFWKRKYELHQVAQQASNIEDLTEVIRASLSVMNKQWADAMKTFHEKFHSL 326

Query: 301 STLIVNHGLDSSAQEEFLSILGGARTSPPVHQFLVNSLGEVGAKRVSKAISGAGSELQLI 360
           STLI+++GL+SS QEEFLS+LGGAR SP ++QFLVNSLGEVG KRV K++ G G ELQ +
Sbjct: 327 STLIIDNGLESSPQEEFLSLLGGARISPALNQFLVNSLGEVGVKRVLKSVCGTGKELQQV 386

Query: 361 VLDHLQPAAEIIGFRMGELLGISRWRARFQGVGLDEKLMHNATEKVGTLLVQVERFMRVL 400
           VLDHLQPAAEIIGFR+GEL G+SRWRAR+QG+GLDE L++ ATE  G LLVQV+RFM VL
Sbjct: 387 VLDHLQPAAEIIGFRIGELRGLSRWRARYQGIGLDEMLLNEATENTGLLLVQVQRFMMVL 446

BLAST of Cucsa.235740 vs. Swiss-Prot
Match: APC4_SCHPO (Anaphase-promoting complex subunit 4 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=cut20 PE=1 SV=2)

HSP 1 Score: 65.5 bits (158), Expect = 1.6e-09
Identity = 83/397 (20.91%), Postives = 161/397 (40.55%), Query Frame = 1

Query: 8   PEKDLLAMVTEDSKILLHRFNWQRLWTIS-PGRSIKSLCWRPDGKVIAVGLEDGTVLLHD 67
           P  +L+A++T  + ++  R N QR+W +         LCW  DG +I VG ++G + + D
Sbjct: 37  PSMELIAILTCSNHLICCRSNSQRIWDVDFHDLEATELCWNHDGNLIVVGFKNGELKIID 96

Query: 68  VENGKLLRSL-KSHAVAVVSLNWVEDSQLITDK-NEILSTYEDRTRRIFPPAPTIPRMPG 127
              G L+     S  +AV+ + W     ++ +K N+ L    D T  +    P +  +P 
Sbjct: 97  SSTGHLVEQRPASRDLAVLMITWAMQETIVNEKRNDFLF---DATAYM----PLLGTLPS 156

Query: 128 LVSGDTGFIDDSEDSFTE---LSNSSQQRFNILCSGDKDGSIRFSIFGVFPIGKIVALSK 187
               +  F   +   F E       + ++  +L   D+ G    ++F  + IG+  +L K
Sbjct: 157 SAKEERIFSSKAIAQFFEPRKREGENNKKVELLSILDERGIRYINMFSSYKIGESDSL-K 216

Query: 188 DFCRLVVMCSGELVGHGHDPRKRQITVQGVHGMHSLVLDTSIFRKRKSELHQVAQQASNI 247
               L V  S  +             V    G  ++ L T      K++L  +   A+  
Sbjct: 217 SALNLGVPISHSIT------NDLAYHVLICKGGTNISLKTLYMPLLKNDLGSIVDIATMS 276

Query: 248 GELTEVIRVSLSVMSKQWSDAMHVFQEKFDSLSTL--IVNHGLDS---SAQEEFLSILGG 307
             +  ++R    V++  + +  +VF+ +   + T   +V+   D+   S Q E    +  
Sbjct: 277 TRMQHLVRYLEEVLNAMYEEFDNVFKSEASFIKTFDALVSKYSDTTFFSLQLELYQFIMN 336

Query: 308 ARTSPPVHQFLVNSLGEVGAKRVSKAISGAGSELQLIVLDHLQPAAEIIGFRMGELLGIS 367
              S  + +++   +G+   K   +A+  + + L +   + + PA E +   +    G S
Sbjct: 337 GIPSDLLKEWINERVGDRVLKNWERAMVNSYTSLIIFCQEFVIPACERLTVLLSSARGKS 396

Query: 368 RWRARFQGVGLDEKLMHNATEKVGTLLVQVERFMRVL 394
            W        LD KL+ +    +G L   V  F+  L
Sbjct: 397 IWGHMKGNTLLDAKLVEDCLATLGYLQNNVFSFLNCL 419

BLAST of Cucsa.235740 vs. Swiss-Prot
Match: APC4_DICDI (Anaphase-promoting complex subunit 4 OS=Dictyostelium discoideum GN=anapc4 PE=3 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 2.8e-09
Identity = 64/299 (21.40%), Postives = 136/299 (45.48%), Query Frame = 1

Query: 132 FIDDSEDSFTELSNSSQQR-FNILCSGDKDGSIRFSIFGVFPIGKIVALSK-----DFCR 191
           + ++S++    L      R F+IL   D  G I    FG+F I  I  LS          
Sbjct: 220 YFENSKEENIYLGGDIYDRPFDILICCDSIGVISLLAFGLFKIVTIDLLSLLKQKYSNTH 279

Query: 192 LVVMCSGEL----VGHGHDPRKRQITVQGVHGMH-SLVLDTSIFRKRKSELHQVAQQASN 251
            ++  S  L    +       K  + ++  +G+  S+ +DTSI  ++++E+H+++ Q   
Sbjct: 280 FLIKPSKSLKILDITLTESLNKLSVMIETDNGLFLSVTIDTSILLEKRNEIHEISLQYFL 339

Query: 252 IGELTEVIRVSLSVMSKQWSDAMHVFQEKFDSLSTLIVNHGLDSSAQEEFLSILGGARTS 311
           + +L + + + +  ++++W +       K+     ++ ++G  SS ++E + +L     S
Sbjct: 340 LFQLQQSLDIHIKEITEKWKETQQQLSTKWVEFEKVLSDYGFSSSIEQELIDLLMCGVPS 399

Query: 312 PPVHQFLVNSLGEVGAKRVSKAISGAGSELQLIVLDHLQPAAEIIGFRMGELLGISRWRA 371
           PP +QF+VN+   +  K++    S   S ++ I++ ++ P+   I   +  L   S    
Sbjct: 400 PPTNQFIVNN---INIKKLKLTESNCNS-IREILIKYILPSFLNIFHIITVLHNNSLEND 459

Query: 372 RFQGVGLDEKLMHNATEKVGTLLVQVERFMRVLSTVLQQVCPMEDEFLLWFSFERKFCC 420
            ++G+ LD   + N  +  G+       F   L ++   +C +E  +  +FS+  K  C
Sbjct: 460 GYKGL-LDTNTVKNILDYCGS-------FGMRLQSLETLICGIESHYTSFFSWLYKVQC 506


HSP 2 Score: 58.9 bits (141), Expect = 1.5e-07
Identity = 33/116 (28.45%), Postives = 59/116 (50.86%), Query Frame = 1

Query: 11  DLLAMVTEDSKILLHRF-NWQR-------LWTISPG------------------------ 70
           DL+A+VT+D +I++HRF  WQ+         TI+                          
Sbjct: 26  DLIALVTKDDQIVIHRFLTWQKLFTINHMSTTINDNNTNNNNNNNNNNNNNNNDNNNDND 85

Query: 71  ---RSIKSLCWRPDGKVIAVGLEDGTVLLHDVENGKLLRSLKSHAVAVVSLNWVED 92
              +SI S+ W P+GK+I++G EDG++ ++++EN KL+    +H   +  L W+++
Sbjct: 86  KSNKSIVSIQWSPNGKMISIGCEDGSIFIYNIENAKLINKSHNHKHPIHKLAWIKE 141

BLAST of Cucsa.235740 vs. Swiss-Prot
Match: WDR61_DANRE (WD repeat-containing protein 61 OS=Danio rerio GN=wdr61 PE=2 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 3.8e-06
Identity = 25/63 (39.68%), Postives = 40/63 (63.49%), Query Frame = 1

Query: 38  GRSIKSLCWRPDGKVIAVGLEDGTVLLHDVENGKLLRSLKSHAVAVVSLNWVEDSQLITD 97
           G+ I S+ + PDGK +A G  DG + + D+  GKLL +L+ HA+ + SL +  DSQL+  
Sbjct: 148 GKFILSIAYSPDGKYLASGAIDGIINIFDIATGKLLHTLEGHAMPIRSLTFSPDSQLLVT 207

Query: 98  KNE 101
            ++
Sbjct: 208 ASD 210

BLAST of Cucsa.235740 vs. Swiss-Prot
Match: WDR61_HUMAN (WD repeat-containing protein 61 OS=Homo sapiens GN=WDR61 PE=1 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 3.8e-06
Identity = 25/63 (39.68%), Postives = 40/63 (63.49%), Query Frame = 1

Query: 38  GRSIKSLCWRPDGKVIAVGLEDGTVLLHDVENGKLLRSLKSHAVAVVSLNWVEDSQLITD 97
           G+ I S+ + PDGK +A G  DG + + D+  GKLL +L+ HA+ + SL +  DSQL+  
Sbjct: 148 GKFILSIAYSPDGKYLASGAIDGIINIFDIATGKLLHTLEGHAMPIRSLTFSPDSQLLVT 207

Query: 98  KNE 101
            ++
Sbjct: 208 ASD 210

BLAST of Cucsa.235740 vs. TrEMBL
Match: A0A0A0LUA0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G386570 PE=4 SV=1)

HSP 1 Score: 773.1 bits (1995), Expect = 1.8e-220
Identity = 398/424 (93.87%), Postives = 398/424 (93.87%), Query Frame = 1

Query: 1   VKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISPGRSIKSLCWRPDGKVIAVGLEDG 60
           VKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISPGRSIKSLCWRPDGKVIAVGLEDG
Sbjct: 24  VKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISPGRSIKSLCWRPDGKVIAVGLEDG 83

Query: 61  TVLLHDVENGKLLRSLKSHAVAVVSLNWVEDSQLITDKNEILSTYEDRTRRIFPPAPTIP 120
           TVLLHDVENGKLLRSLKSHAVAVVSLNWVEDSQLITDKNEILSTYEDRTRRIFPPAPTIP
Sbjct: 84  TVLLHDVENGKLLRSLKSHAVAVVSLNWVEDSQLITDKNEILSTYEDRTRRIFPPAPTIP 143

Query: 121 RMPGLVSGDTGFIDDSEDSFTELSNSSQQRFNILCSGDKDGSIRFSIFGVFPIGKI---- 180
           RMPGLVSGDTGFIDDSEDSFTELSNSSQQRFNILCSGDKDGSIRFSIFGVFPIGKI    
Sbjct: 144 RMPGLVSGDTGFIDDSEDSFTELSNSSQQRFNILCSGDKDGSIRFSIFGVFPIGKINIHE 203

Query: 181 ---------------------VALSKDFCRLVVMCSGELVGHGHDPRKRQITVQGVHGMH 240
                                VALSKDFCRLVVMCSGELVGHGHDPRKRQITVQGVHGMH
Sbjct: 204 LHIPLQDASASCHLLNAEIYKVALSKDFCRLVVMCSGELVGHGHDPRKRQITVQGVHGMH 263

Query: 241 SLVLDTSIFRKRKSELHQVAQQASNIGELTEVIRVSLSVMSKQWSDAMHVFQEKFDSLST 300
           SLVLDTSIFRKRKSELHQVAQQASNIGELTEVIRVSLSVMSKQWSDAMH FQEKFDSLST
Sbjct: 264 SLVLDTSIFRKRKSELHQVAQQASNIGELTEVIRVSLSVMSKQWSDAMHAFQEKFDSLST 323

Query: 301 LIVNHGLDSSAQEEFLSILGGARTSPPVHQFLVNSLGEVGAKRVSKAISGAGSELQLIVL 360
           LIVNHGLDSSAQEEFLSILGGARTSPPVHQFLVNSLGEVGAKRVSKAISGAGSELQLIVL
Sbjct: 324 LIVNHGLDSSAQEEFLSILGGARTSPPVHQFLVNSLGEVGAKRVSKAISGAGSELQLIVL 383

Query: 361 DHLQPAAEIIGFRMGELLGISRWRARFQGVGLDEKLMHNATEKVGTLLVQVERFMRVLST 400
           DHLQPAAEIIGFRMGELLGISRWRARFQGVGLDEKLMHNATEKVGTLLVQVERFMRVLST
Sbjct: 384 DHLQPAAEIIGFRMGELLGISRWRARFQGVGLDEKLMHNATEKVGTLLVQVERFMRVLST 443

BLAST of Cucsa.235740 vs. TrEMBL
Match: M5WNK9_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa001703mg PE=4 SV=1)

HSP 1 Score: 617.8 bits (1592), Expect = 9.6e-174
Identity = 315/426 (73.94%), Postives = 344/426 (80.75%), Query Frame = 1

Query: 1   VKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISPGRSIKSLCWRPDGKVIAVGLEDG 60
           +KIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWT+SPG+SI SLCWRPDGK IAVGLEDG
Sbjct: 24  IKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTVSPGKSITSLCWRPDGKAIAVGLEDG 83

Query: 61  TVLLHDVENGKLLRSLKSHAVAVVSLNWVEDSQLITDKNEILSTYEDRTRRIFPPAPTIP 120
           TV LHDVENGKLLRSLKSH VAVVSLNW ED Q+  D+   +STYEDRT   FPP P +P
Sbjct: 84  TVSLHDVENGKLLRSLKSHGVAVVSLNWEEDGQMNKDEQGSISTYEDRTPHYFPPPPRVP 143

Query: 121 RMPGLVSGDTGFIDDSEDSFTELSNSSQQRFNILCSGDKDGSIRFSIFGVFPIGKI---- 180
           RMPGLVSGDTGFIDDSEDSF ELSNSSQQRFNILCSGDKDG I FSIFG+F IGKI    
Sbjct: 144 RMPGLVSGDTGFIDDSEDSFRELSNSSQQRFNILCSGDKDGFICFSIFGIFSIGKINIHN 203

Query: 181 -----------------------VALSKDFCRLVVMCSGELVGHGHDPRKRQITVQGVHG 240
                                  VALSKD C L+V+CSGEL     +   R +T  GV G
Sbjct: 204 FFVPTLLMDTKAECRLQNASVHKVALSKDLCHLIVICSGELSEDRKESENRSMTQHGVRG 263

Query: 241 MHSLVLDTSIFRKRKSELHQVAQQASNIGELTEVIRVSLSVMSKQWSDAMHVFQEKFDSL 300
           +H  VLDTSIF KRK+ELHQVAQQASNI EL EVIR SLSVM KQWSDAMH F EKFDSL
Sbjct: 264 LHCTVLDTSIFWKRKNELHQVAQQASNIEELVEVIRASLSVMHKQWSDAMHTFHEKFDSL 323

Query: 301 STLIVNHGLDSSAQEEFLSILGGARTSPPVHQFLVNSLGEVGAKRVSKAISGAGSELQLI 360
           S LI+++GLDS  QEEFLS+LGGARTSP VHQFLVNSLGEVG KRVSKA+ GAG ELQLI
Sbjct: 324 SNLIIDNGLDSCPQEEFLSLLGGARTSPAVHQFLVNSLGEVGVKRVSKAVCGAGKELQLI 383

Query: 361 VLDHLQPAAEIIGFRMGELLGISRWRARFQGVGLDEKLMHNATEKVGTLLVQVERFMRVL 400
           VL+HLQPAAEII FRMGEL G+SRWRAR+QG+GLDE L++NATEK G +L+QVERF+RVL
Sbjct: 384 VLNHLQPAAEIIAFRMGELRGLSRWRARYQGIGLDEALINNATEKAGMILIQVERFIRVL 443

BLAST of Cucsa.235740 vs. TrEMBL
Match: E0CVY8_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_00s0181g00210 PE=4 SV=1)

HSP 1 Score: 617.8 bits (1592), Expect = 9.6e-174
Identity = 318/426 (74.65%), Postives = 347/426 (81.46%), Query Frame = 1

Query: 1   VKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISPGRSIKSLCWRPDGKVIAVGLEDG 60
           +KIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISPGR I SLCWRPDGK IAVGLEDG
Sbjct: 24  IKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISPGRCITSLCWRPDGKAIAVGLEDG 83

Query: 61  TVLLHDVENGKLLRSLKSHAVAVVSLNWVEDSQLITDKNEILSTYEDRTRRIFPPAPTIP 120
           TV LHDVENGKLLRS+KSH VAVV LNW ED QLI D   IL+ YEDRT R FPPAP +P
Sbjct: 84  TVSLHDVENGKLLRSMKSHTVAVVCLNWEEDGQLIGDSGNILA-YEDRTSRFFPPAPRVP 143

Query: 121 RMPGLVSGDTGFIDDSEDSFTELSNSSQQRFNILCSGDKDGSIRFSIFGVFPIGKI---- 180
           RMPGLVSGDTGF+DDSEDSF ELSNSS QRFNILCSGDKDGSI FSIFG+FPIGKI    
Sbjct: 144 RMPGLVSGDTGFMDDSEDSFGELSNSSHQRFNILCSGDKDGSICFSIFGMFPIGKINIHE 203

Query: 181 -----------------------VALSKDFCRLVVMCSGELVGHGHDPRKRQITVQGVHG 240
                                  VALSKD C L+V CSGELV    + R R I   G+ G
Sbjct: 204 FSVSTLLMDNQVTYRLLNASICKVALSKDLCHLIVTCSGELVEESVESRDRHIFGHGLLG 263

Query: 241 MHSLVLDTSIFRKRKSELHQVAQQASNIGELTEVIRVSLSVMSKQWSDAMHVFQEKFDSL 300
           +HSLVLDTSIF KRK+ELHQVAQQASNI +LTEVIR SLSVM KQWSDAMH+F EKFDSL
Sbjct: 264 LHSLVLDTSIFWKRKNELHQVAQQASNIEDLTEVIRASLSVMCKQWSDAMHMFHEKFDSL 323

Query: 301 STLIVNHGLDSSAQEEFLSILGGARTSPPVHQFLVNSLGEVGAKRVSKAISGAGSELQLI 360
           S+LI++HGLDSS QEEFLS+L GARTSPP+HQFLV+SLGEVG KRVSKA+S AG ELQ I
Sbjct: 324 SSLIIDHGLDSSPQEEFLSLLCGARTSPPLHQFLVSSLGEVGLKRVSKAVSNAGKELQFI 383

Query: 361 VLDHLQPAAEIIGFRMGELLGISRWRARFQGVGLDEKLMHNATEKVGTLLVQVERFMRVL 400
           VLDHLQPAAEIIGFRMGEL G+SRWR R++ +GLDE+L+ NATEK G LLVQVERFM +L
Sbjct: 384 VLDHLQPAAEIIGFRMGELRGLSRWRTRYKVIGLDERLIDNATEKAGMLLVQVERFMMIL 443

BLAST of Cucsa.235740 vs. TrEMBL
Match: A0A0D2RFQ0_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_011G022700 PE=4 SV=1)

HSP 1 Score: 617.1 bits (1590), Expect = 1.6e-173
Identity = 316/426 (74.18%), Postives = 346/426 (81.22%), Query Frame = 1

Query: 1   VKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISPGRSIKSLCWRPDGKVIAVGLEDG 60
           +KIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTI PGR I SLCWRPDGK IAVGLEDG
Sbjct: 24  IKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTIFPGRCITSLCWRPDGKAIAVGLEDG 83

Query: 61  TVLLHDVENGKLLRSLKSHAVAVVSLNWVEDSQLITDKNEILSTYEDRTRRIFPPAPTIP 120
           T+ LHDVENGKLLRSLKSH VAVVSLNW ED Q+I D +   STYEDRT R FPPAP +P
Sbjct: 84  TISLHDVENGKLLRSLKSHTVAVVSLNWEEDGQVIKDDSVNNSTYEDRTSRFFPPAPRVP 143

Query: 121 RMPGLVSGDTGFIDDSEDSFTELSNSSQQRFNILCSGDKDGSIRFSIFGVFPIGKI---- 180
           RMPGL  GDTGF+DDS+DSF ELSNSS QRFNILCSGDKDGS  FSIFG+FPIGKI    
Sbjct: 144 RMPGLAPGDTGFMDDSDDSFRELSNSSYQRFNILCSGDKDGSTCFSIFGIFPIGKINIHN 203

Query: 181 -----------------------VALSKDFCRLVVMCSGELVGHGHDPRKRQITVQGVHG 240
                                  +ALSKD C L+VMCSGEL     +  +RQ+   GVHG
Sbjct: 204 LSIPTPYATEPATCRLSHASISKIALSKDLCHLIVMCSGELNHDEVESAERQL---GVHG 263

Query: 241 MHSLVLDTSIFRKRKSELHQVAQQASNIGELTEVIRVSLSVMSKQWSDAMHVFQEKFDSL 300
            H L+LDTSIF KRK+ELHQVAQQASNI +L EVIR SLSVMSKQWSDAMH F+EKFDSL
Sbjct: 264 CHCLLLDTSIFWKRKNELHQVAQQASNIEDLIEVIRSSLSVMSKQWSDAMHTFREKFDSL 323

Query: 301 STLIVNHGLDSSAQEEFLSILGGARTSPPVHQFLVNSLGEVGAKRVSKAISGAGSELQLI 360
           S+LI++HGLDSS QEEFLS+LGGARTSPPVHQFLVNSLGE G KRVSK + GAG ELQ +
Sbjct: 324 SSLIIDHGLDSSPQEEFLSLLGGARTSPPVHQFLVNSLGESGVKRVSKVVCGAGKELQHV 383

Query: 361 VLDHLQPAAEIIGFRMGELLGISRWRARFQGVGLDEKLMHNATEKVGTLLVQVERFMRVL 400
           VLDHLQPAAEIIGFRMGEL G+SRWR RFQG+GLDE L++NATEK G LLVQVERFMRVL
Sbjct: 384 VLDHLQPAAEIIGFRMGELRGLSRWRTRFQGIGLDETLINNATEKSGILLVQVERFMRVL 443

BLAST of Cucsa.235740 vs. TrEMBL
Match: A0A0B0P1E8_GOSAR (Anaphase-promoting complex subunit 4-like protein OS=Gossypium arboreum GN=F383_05168 PE=4 SV=1)

HSP 1 Score: 616.7 bits (1589), Expect = 2.1e-173
Identity = 317/426 (74.41%), Postives = 344/426 (80.75%), Query Frame = 1

Query: 1   VKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISPGRSIKSLCWRPDGKVIAVGLEDG 60
           +KIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTI PGR I SLCWRPDGK IAVGLEDG
Sbjct: 24  IKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTIFPGRCITSLCWRPDGKAIAVGLEDG 83

Query: 61  TVLLHDVENGKLLRSLKSHAVAVVSLNWVEDSQLITDKNEILSTYEDRTRRIFPPAPTIP 120
           T+ LHDVENGKLLRSLKSH VAVVSLNW ED Q+I D +   STYEDRT R FPPAP +P
Sbjct: 84  TISLHDVENGKLLRSLKSHTVAVVSLNWEEDGQVIKDDSVNNSTYEDRTSRFFPPAPRVP 143

Query: 121 RMPGLVSGDTGFIDDSEDSFTELSNSSQQRFNILCSGDKDGSIRFSIFGVFPIGKI---- 180
           RMPGL  GDTGF+DDS+DSF ELSNSS QRFNILCSGDKDGSI FSIFG+FPIGKI    
Sbjct: 144 RMPGLAPGDTGFMDDSDDSFRELSNSSYQRFNILCSGDKDGSICFSIFGIFPIGKINIHN 203

Query: 181 -----------------------VALSKDFCRLVVMCSGELVGHGHDPRKRQITVQGVHG 240
                                  VALSKD C L+VMCSGE+    HD  +      G+HG
Sbjct: 204 LSIPTPFATEPATCRLSHASISKVALSKDLCHLIVMCSGEV---NHDEVESAEGQLGMHG 263

Query: 241 MHSLVLDTSIFRKRKSELHQVAQQASNIGELTEVIRVSLSVMSKQWSDAMHVFQEKFDSL 300
            H L LDTSIF KRK+ELHQVAQQASNI +L EVIR SLSVMSKQWSDAMH F+EKFDSL
Sbjct: 264 CHCLRLDTSIFWKRKNELHQVAQQASNIEDLIEVIRASLSVMSKQWSDAMHTFREKFDSL 323

Query: 301 STLIVNHGLDSSAQEEFLSILGGARTSPPVHQFLVNSLGEVGAKRVSKAISGAGSELQLI 360
           S+LI++HGLDSS QEEFLS+LGGARTSPPVHQFLVNSLGE G KRVSK + GAG ELQ +
Sbjct: 324 SSLIIDHGLDSSPQEEFLSLLGGARTSPPVHQFLVNSLGESGVKRVSKVVCGAGKELQHV 383

Query: 361 VLDHLQPAAEIIGFRMGELLGISRWRARFQGVGLDEKLMHNATEKVGTLLVQVERFMRVL 400
           VLDHLQPAAEIIGFRMGEL G+SRWR RFQG+GLDE L++NATEK G LLVQVERFMRVL
Sbjct: 384 VLDHLQPAAEIIGFRMGELRGLSRWRTRFQGIGLDETLINNATEKSGILLVQVERFMRVL 443

BLAST of Cucsa.235740 vs. TAIR10
Match: AT4G21530.1 (AT4G21530.1 Transducin/WD40 repeat-like superfamily protein)

HSP 1 Score: 572.4 bits (1474), Expect = 2.3e-163
Identity = 290/426 (68.08%), Postives = 336/426 (78.87%), Query Frame = 1

Query: 1   VKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISPGRSIKSLCWRPDGKVIAVGLEDG 60
           +KIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISPG+ + SLCWRPDGK IAVGLEDG
Sbjct: 27  IKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISPGKPVTSLCWRPDGKAIAVGLEDG 86

Query: 61  TVLLHDVENGKLLRSLKSHAVAVVSLNWVEDSQLITDKNEILSTYEDRTRRIFPPAPTIP 120
           T+ LHDVENGKLLR+LK H VAVV LNW ED Q  TD++   S YEDRT R FPPAP  P
Sbjct: 87  TISLHDVENGKLLRNLKPHDVAVVCLNWEEDGQSNTDESGNFSVYEDRTSRFFPPAPRPP 146

Query: 121 RMPGLVSGDTGFIDDSEDSFTELSNSSQQRFNILCSGDKDGSIRFSIFGVFPIGKI---- 180
           +MPGLV+GD+ F+DD EDS  ELSN+S ++FNILC+GD+DG+I FSIFG+F IGKI    
Sbjct: 147 KMPGLVAGDSSFMDDGEDSLAELSNTSFRKFNILCTGDRDGNICFSIFGIFQIGKINIHE 206

Query: 181 -----------------------VALSKDFCRLVVMCSGELVGHGHDPRKRQITVQGVHG 240
                                  VALSKD CRLVVMC+GEL      PR+ +I VQ + G
Sbjct: 207 LSLPVPHLDEHASCKLFNASIYKVALSKDLCRLVVMCTGELKDCDIKPREEKINVQDLPG 266

Query: 241 MHSLVLDTSIFRKRKSELHQVAQQASNIGELTEVIRVSLSVMSKQWSDAMHVFQEKFDSL 300
           +H L +DTSIF KRK ELHQVAQQASNI +LTEVIR SLSVM+KQW+DAM  F EKF SL
Sbjct: 267 LHCLAMDTSIFWKRKYELHQVAQQASNIEDLTEVIRASLSVMNKQWADAMKTFHEKFHSL 326

Query: 301 STLIVNHGLDSSAQEEFLSILGGARTSPPVHQFLVNSLGEVGAKRVSKAISGAGSELQLI 360
           STLI+++GL+SS QEEFLS+LGGAR SP ++QFLVNSLGEVG KRV K++ G G ELQ +
Sbjct: 327 STLIIDNGLESSPQEEFLSLLGGARISPALNQFLVNSLGEVGVKRVLKSVCGTGKELQQV 386

Query: 361 VLDHLQPAAEIIGFRMGELLGISRWRARFQGVGLDEKLMHNATEKVGTLLVQVERFMRVL 400
           VLDHLQPAAEIIGFR+GEL G+SRWRAR+QG+GLDE L++ ATE  G LLVQV+RFM VL
Sbjct: 387 VLDHLQPAAEIIGFRIGELRGLSRWRARYQGIGLDEMLLNEATENTGLLLVQVQRFMMVL 446

BLAST of Cucsa.235740 vs. NCBI nr
Match: gi|778660566|ref|XP_011656470.1| (PREDICTED: anaphase-promoting complex subunit 4 [Cucumis sativus])

HSP 1 Score: 773.1 bits (1995), Expect = 2.6e-220
Identity = 398/424 (93.87%), Postives = 398/424 (93.87%), Query Frame = 1

Query: 1   VKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISPGRSIKSLCWRPDGKVIAVGLEDG 60
           VKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISPGRSIKSLCWRPDGKVIAVGLEDG
Sbjct: 24  VKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISPGRSIKSLCWRPDGKVIAVGLEDG 83

Query: 61  TVLLHDVENGKLLRSLKSHAVAVVSLNWVEDSQLITDKNEILSTYEDRTRRIFPPAPTIP 120
           TVLLHDVENGKLLRSLKSHAVAVVSLNWVEDSQLITDKNEILSTYEDRTRRIFPPAPTIP
Sbjct: 84  TVLLHDVENGKLLRSLKSHAVAVVSLNWVEDSQLITDKNEILSTYEDRTRRIFPPAPTIP 143

Query: 121 RMPGLVSGDTGFIDDSEDSFTELSNSSQQRFNILCSGDKDGSIRFSIFGVFPIGKI---- 180
           RMPGLVSGDTGFIDDSEDSFTELSNSSQQRFNILCSGDKDGSIRFSIFGVFPIGKI    
Sbjct: 144 RMPGLVSGDTGFIDDSEDSFTELSNSSQQRFNILCSGDKDGSIRFSIFGVFPIGKINIHE 203

Query: 181 ---------------------VALSKDFCRLVVMCSGELVGHGHDPRKRQITVQGVHGMH 240
                                VALSKDFCRLVVMCSGELVGHGHDPRKRQITVQGVHGMH
Sbjct: 204 LHIPLQDASASCHLLNAEIYKVALSKDFCRLVVMCSGELVGHGHDPRKRQITVQGVHGMH 263

Query: 241 SLVLDTSIFRKRKSELHQVAQQASNIGELTEVIRVSLSVMSKQWSDAMHVFQEKFDSLST 300
           SLVLDTSIFRKRKSELHQVAQQASNIGELTEVIRVSLSVMSKQWSDAMH FQEKFDSLST
Sbjct: 264 SLVLDTSIFRKRKSELHQVAQQASNIGELTEVIRVSLSVMSKQWSDAMHAFQEKFDSLST 323

Query: 301 LIVNHGLDSSAQEEFLSILGGARTSPPVHQFLVNSLGEVGAKRVSKAISGAGSELQLIVL 360
           LIVNHGLDSSAQEEFLSILGGARTSPPVHQFLVNSLGEVGAKRVSKAISGAGSELQLIVL
Sbjct: 324 LIVNHGLDSSAQEEFLSILGGARTSPPVHQFLVNSLGEVGAKRVSKAISGAGSELQLIVL 383

Query: 361 DHLQPAAEIIGFRMGELLGISRWRARFQGVGLDEKLMHNATEKVGTLLVQVERFMRVLST 400
           DHLQPAAEIIGFRMGELLGISRWRARFQGVGLDEKLMHNATEKVGTLLVQVERFMRVLST
Sbjct: 384 DHLQPAAEIIGFRMGELLGISRWRARFQGVGLDEKLMHNATEKVGTLLVQVERFMRVLST 443

BLAST of Cucsa.235740 vs. NCBI nr
Match: gi|700210277|gb|KGN65373.1| (hypothetical protein Csa_1G386570 [Cucumis sativus])

HSP 1 Score: 773.1 bits (1995), Expect = 2.6e-220
Identity = 398/424 (93.87%), Postives = 398/424 (93.87%), Query Frame = 1

Query: 1   VKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISPGRSIKSLCWRPDGKVIAVGLEDG 60
           VKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISPGRSIKSLCWRPDGKVIAVGLEDG
Sbjct: 24  VKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISPGRSIKSLCWRPDGKVIAVGLEDG 83

Query: 61  TVLLHDVENGKLLRSLKSHAVAVVSLNWVEDSQLITDKNEILSTYEDRTRRIFPPAPTIP 120
           TVLLHDVENGKLLRSLKSHAVAVVSLNWVEDSQLITDKNEILSTYEDRTRRIFPPAPTIP
Sbjct: 84  TVLLHDVENGKLLRSLKSHAVAVVSLNWVEDSQLITDKNEILSTYEDRTRRIFPPAPTIP 143

Query: 121 RMPGLVSGDTGFIDDSEDSFTELSNSSQQRFNILCSGDKDGSIRFSIFGVFPIGKI---- 180
           RMPGLVSGDTGFIDDSEDSFTELSNSSQQRFNILCSGDKDGSIRFSIFGVFPIGKI    
Sbjct: 144 RMPGLVSGDTGFIDDSEDSFTELSNSSQQRFNILCSGDKDGSIRFSIFGVFPIGKINIHE 203

Query: 181 ---------------------VALSKDFCRLVVMCSGELVGHGHDPRKRQITVQGVHGMH 240
                                VALSKDFCRLVVMCSGELVGHGHDPRKRQITVQGVHGMH
Sbjct: 204 LHIPLQDASASCHLLNAEIYKVALSKDFCRLVVMCSGELVGHGHDPRKRQITVQGVHGMH 263

Query: 241 SLVLDTSIFRKRKSELHQVAQQASNIGELTEVIRVSLSVMSKQWSDAMHVFQEKFDSLST 300
           SLVLDTSIFRKRKSELHQVAQQASNIGELTEVIRVSLSVMSKQWSDAMH FQEKFDSLST
Sbjct: 264 SLVLDTSIFRKRKSELHQVAQQASNIGELTEVIRVSLSVMSKQWSDAMHAFQEKFDSLST 323

Query: 301 LIVNHGLDSSAQEEFLSILGGARTSPPVHQFLVNSLGEVGAKRVSKAISGAGSELQLIVL 360
           LIVNHGLDSSAQEEFLSILGGARTSPPVHQFLVNSLGEVGAKRVSKAISGAGSELQLIVL
Sbjct: 324 LIVNHGLDSSAQEEFLSILGGARTSPPVHQFLVNSLGEVGAKRVSKAISGAGSELQLIVL 383

Query: 361 DHLQPAAEIIGFRMGELLGISRWRARFQGVGLDEKLMHNATEKVGTLLVQVERFMRVLST 400
           DHLQPAAEIIGFRMGELLGISRWRARFQGVGLDEKLMHNATEKVGTLLVQVERFMRVLST
Sbjct: 384 DHLQPAAEIIGFRMGELLGISRWRARFQGVGLDEKLMHNATEKVGTLLVQVERFMRVLST 443

BLAST of Cucsa.235740 vs. NCBI nr
Match: gi|659126790|ref|XP_008463366.1| (PREDICTED: anaphase-promoting complex subunit 4 [Cucumis melo])

HSP 1 Score: 768.5 bits (1983), Expect = 6.3e-219
Identity = 396/424 (93.40%), Postives = 397/424 (93.63%), Query Frame = 1

Query: 1   VKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISPGRSIKSLCWRPDGKVIAVGLEDG 60
           VKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISPGRSIKSLCWRPDGKVIAVGLEDG
Sbjct: 24  VKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISPGRSIKSLCWRPDGKVIAVGLEDG 83

Query: 61  TVLLHDVENGKLLRSLKSHAVAVVSLNWVEDSQLITDKNEILSTYEDRTRRIFPPAPTIP 120
           TVLLHDVENGKLLRSLKSHAVAVVSLNWVEDSQLITDKNEILSTYEDRTRRIFPPAPTIP
Sbjct: 84  TVLLHDVENGKLLRSLKSHAVAVVSLNWVEDSQLITDKNEILSTYEDRTRRIFPPAPTIP 143

Query: 121 RMPGLVSGDTGFIDDSEDSFTELSNSSQQRFNILCSGDKDGSIRFSIFGVFPIGKI---- 180
           RMPGLVSGDTGFIDDSEDSFTELSNSSQQRFNILCSGDKDGSI FSIFGVFPIGKI    
Sbjct: 144 RMPGLVSGDTGFIDDSEDSFTELSNSSQQRFNILCSGDKDGSICFSIFGVFPIGKINIHE 203

Query: 181 ---------------------VALSKDFCRLVVMCSGELVGHGHDPRKRQITVQGVHGMH 240
                                VALSKDFCRLVVMCSGELVGHGHDPRKRQITVQGVHGMH
Sbjct: 204 LHIPLQDASASCHLLNAEIYKVALSKDFCRLVVMCSGELVGHGHDPRKRQITVQGVHGMH 263

Query: 241 SLVLDTSIFRKRKSELHQVAQQASNIGELTEVIRVSLSVMSKQWSDAMHVFQEKFDSLST 300
           SLVLDTSIFRKRKSELHQVAQQASNIGELTEVIRVSLSVMSKQWSDAMH F+EKFDSLST
Sbjct: 264 SLVLDTSIFRKRKSELHQVAQQASNIGELTEVIRVSLSVMSKQWSDAMHTFREKFDSLST 323

Query: 301 LIVNHGLDSSAQEEFLSILGGARTSPPVHQFLVNSLGEVGAKRVSKAISGAGSELQLIVL 360
           LIVNHGLDSSAQEEFLSILGGARTSPPVHQFLVNSLGEVGAKRVSKAISGAGSELQLIVL
Sbjct: 324 LIVNHGLDSSAQEEFLSILGGARTSPPVHQFLVNSLGEVGAKRVSKAISGAGSELQLIVL 383

Query: 361 DHLQPAAEIIGFRMGELLGISRWRARFQGVGLDEKLMHNATEKVGTLLVQVERFMRVLST 400
           DHLQPAAEIIGFRMGELLGISRWRARFQGVGLDEKLMHNATEKVGTLLVQVERFMRVLST
Sbjct: 384 DHLQPAAEIIGFRMGELLGISRWRARFQGVGLDEKLMHNATEKVGTLLVQVERFMRVLST 443

BLAST of Cucsa.235740 vs. NCBI nr
Match: gi|657983660|ref|XP_008383915.1| (PREDICTED: anaphase-promoting complex subunit 4-like [Malus domestica])

HSP 1 Score: 627.1 bits (1616), Expect = 2.3e-176
Identity = 317/422 (75.12%), Postives = 344/422 (81.52%), Query Frame = 1

Query: 1   VKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISPGRSIKSLCWRPDGKVIAVGLEDG 60
           +KIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISP RSI SLCWRPDGK IAVGLEDG
Sbjct: 24  IKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISPARSISSLCWRPDGKAIAVGLEDG 83

Query: 61  TVLLHDVENGKLLRSLKSHAVAVVSLNWVEDSQLITDKNEILSTYEDRTRRIFPPAPTIP 120
           TV LHDVENGKLLRSLKSH+VAVVSLNW ED     D++  +STYEDRT R FPP P +P
Sbjct: 84  TVSLHDVENGKLLRSLKSHSVAVVSLNWEEDGHTTRDEHGSISTYEDRTSRFFPPPPRVP 143

Query: 121 RMPGLVSGDTGFIDDSEDSFTELSNSSQQRFNILCSGDKDGSIRFSIFGVFPIGKI---- 180
           RMPGLVSGDTGFIDDSEDSF ELSNSSQQRFNILCSGDKDG I FSIFG+FPIGKI    
Sbjct: 144 RMPGLVSGDTGFIDDSEDSFQELSNSSQQRFNILCSGDKDGVICFSIFGIFPIGKINIHN 203

Query: 181 -----------------------VALSKDFCRLVVMCSGELVGHGHDPRKRQITVQGVHG 240
                                  VALSKD C L+V CSGEL     +   R++T  G+HG
Sbjct: 204 FCVPNPLTDAKAECRLLNASVCKVALSKDLCHLIVTCSGELTDDREESESRRVTQHGIHG 263

Query: 241 MHSLVLDTSIFRKRKSELHQVAQQASNIGELTEVIRVSLSVMSKQWSDAMHVFQEKFDSL 300
           +H +VLDTSIF KRK+ELHQVAQQASNI EL EVIR SLSVM KQWSDAMH F EKFDSL
Sbjct: 264 LHCMVLDTSIFWKRKNELHQVAQQASNIEELVEVIRASLSVMHKQWSDAMHTFHEKFDSL 323

Query: 301 STLIVNHGLDSSAQEEFLSILGGARTSPPVHQFLVNSLGEVGAKRVSKAISGAGSELQLI 360
           S LIV+HGLDS  QEEFLS+LGGARTSPPVHQFLVNSLGEVG KRVSKA+ GAG ELQLI
Sbjct: 324 SNLIVDHGLDSCPQEEFLSLLGGARTSPPVHQFLVNSLGEVGVKRVSKAVCGAGKELQLI 383

Query: 361 VLDHLQPAAEIIGFRMGELLGISRWRARFQGVGLDEKLMHNATEKVGTLLVQVERFMRVL 396
           VL+HLQPAAEII FRMGEL G+SRWRAR+QG+GLDE L++NATEK G +L+QVERF+RVL
Sbjct: 384 VLNHLQPAAEIIAFRMGELRGLSRWRARYQGIGLDETLINNATEKAGMILLQVERFIRVL 443

BLAST of Cucsa.235740 vs. NCBI nr
Match: gi|645236881|ref|XP_008224948.1| (PREDICTED: anaphase-promoting complex subunit 4 [Prunus mume])

HSP 1 Score: 624.0 bits (1608), Expect = 1.9e-175
Identity = 317/426 (74.41%), Postives = 346/426 (81.22%), Query Frame = 1

Query: 1   VKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISPGRSIKSLCWRPDGKVIAVGLEDG 60
           +KIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWT+SPG+SI SLCWRPDGK IAVGLEDG
Sbjct: 24  IKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTVSPGKSITSLCWRPDGKAIAVGLEDG 83

Query: 61  TVLLHDVENGKLLRSLKSHAVAVVSLNWVEDSQLITDKNEILSTYEDRTRRIFPPAPTIP 120
           TV LHDVENGKLLRSLKSH VAVVSLNW ED Q+  D+   +STYEDRT   FPP P +P
Sbjct: 84  TVSLHDVENGKLLRSLKSHGVAVVSLNWEEDGQMTKDEQGSISTYEDRTPHFFPPPPRVP 143

Query: 121 RMPGLVSGDTGFIDDSEDSFTELSNSSQQRFNILCSGDKDGSIRFSIFGVFPIGKI---- 180
           RMPGLVSGDTGFIDDSEDSF ELSNSSQQRFNILCSGDKDG I FSIFG+FPIGKI    
Sbjct: 144 RMPGLVSGDTGFIDDSEDSFRELSNSSQQRFNILCSGDKDGFICFSIFGIFPIGKINIHN 203

Query: 181 -----------------------VALSKDFCRLVVMCSGELVGHGHDPRKRQITVQGVHG 240
                                  VALSKD C L+V+CSGEL     +   R +T  GV G
Sbjct: 204 FCVPTPLMDTKAECRLQNASVHKVALSKDLCHLIVICSGELSEDRKESENRSMTQHGVRG 263

Query: 241 MHSLVLDTSIFRKRKSELHQVAQQASNIGELTEVIRVSLSVMSKQWSDAMHVFQEKFDSL 300
           +H  VLDTSIF KRK+ELHQVAQQASNI EL EVIR SLSVM KQWSDAMH F EKFDSL
Sbjct: 264 LHCTVLDTSIFWKRKNELHQVAQQASNIEELVEVIRASLSVMHKQWSDAMHTFHEKFDSL 323

Query: 301 STLIVNHGLDSSAQEEFLSILGGARTSPPVHQFLVNSLGEVGAKRVSKAISGAGSELQLI 360
           STLI+++GLDS  QEEFLS+LGGARTSP VHQFLVNSLGEVG KRVSKA+ GAG ELQLI
Sbjct: 324 STLIIDNGLDSCPQEEFLSLLGGARTSPAVHQFLVNSLGEVGVKRVSKAVCGAGKELQLI 383

Query: 361 VLDHLQPAAEIIGFRMGELLGISRWRARFQGVGLDEKLMHNATEKVGTLLVQVERFMRVL 400
           VL+HLQPAAEII FRMGEL G+SRWRAR+QG+GLDE L++NATEK G +L+QVERF+RVL
Sbjct: 384 VLNHLQPAAEIIAFRMGELRGLSRWRARYQGIGLDEALINNATEKAGMILIQVERFIRVL 443

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
APC4_ARATH4.1e-16268.08Anaphase-promoting complex subunit 4 OS=Arabidopsis thaliana GN=APC4 PE=2 SV=2[more]
APC4_SCHPO1.6e-0920.91Anaphase-promoting complex subunit 4 OS=Schizosaccharomyces pombe (strain 972 / ... [more]
APC4_DICDI2.8e-0921.40Anaphase-promoting complex subunit 4 OS=Dictyostelium discoideum GN=anapc4 PE=3 ... [more]
WDR61_DANRE3.8e-0639.68WD repeat-containing protein 61 OS=Danio rerio GN=wdr61 PE=2 SV=1[more]
WDR61_HUMAN3.8e-0639.68WD repeat-containing protein 61 OS=Homo sapiens GN=WDR61 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LUA0_CUCSA1.8e-22093.87Uncharacterized protein OS=Cucumis sativus GN=Csa_1G386570 PE=4 SV=1[more]
M5WNK9_PRUPE9.6e-17473.94Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa001703mg PE=4 SV=1[more]
E0CVY8_VITVI9.6e-17474.65Putative uncharacterized protein OS=Vitis vinifera GN=VIT_00s0181g00210 PE=4 SV=... [more]
A0A0D2RFQ0_GOSRA1.6e-17374.18Uncharacterized protein OS=Gossypium raimondii GN=B456_011G022700 PE=4 SV=1[more]
A0A0B0P1E8_GOSAR2.1e-17374.41Anaphase-promoting complex subunit 4-like protein OS=Gossypium arboreum GN=F383_... [more]
Match NameE-valueIdentityDescription
AT4G21530.12.3e-16368.08 Transducin/WD40 repeat-like superfamily protein[more]
Match NameE-valueIdentityDescription
gi|778660566|ref|XP_011656470.1|2.6e-22093.87PREDICTED: anaphase-promoting complex subunit 4 [Cucumis sativus][more]
gi|700210277|gb|KGN65373.1|2.6e-22093.87hypothetical protein Csa_1G386570 [Cucumis sativus][more]
gi|659126790|ref|XP_008463366.1|6.3e-21993.40PREDICTED: anaphase-promoting complex subunit 4 [Cucumis melo][more]
gi|657983660|ref|XP_008383915.1|2.3e-17675.12PREDICTED: anaphase-promoting complex subunit 4-like [Malus domestica][more]
gi|645236881|ref|XP_008224948.1|1.9e-17574.41PREDICTED: anaphase-promoting complex subunit 4 [Prunus mume][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001680WD40_repeat
IPR015943WD40/YVTN_repeat-like_dom_sf
IPR017986WD40_repeat_dom
IPR024789APC4
IPR024790APC4_long_dom
IPR024977Apc4_WD40_dom
Vocabulary: Molecular Function
TermDefinition
GO:0005515protein binding
Vocabulary: Cellular Component
TermDefinition
GO:0005680anaphase-promoting complex
Vocabulary: Biological Process
TermDefinition
GO:0030071regulation of mitotic metaphase/anaphase transition
GO:0031145anaphase-promoting complex-dependent catabolic process
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009793 embryo development ending in seed dormancy
biological_process GO:0048481 plant ovule development
biological_process GO:0030071 regulation of mitotic metaphase/anaphase transition
biological_process GO:0031145 anaphase-promoting complex-dependent catabolic process
cellular_component GO:0005680 anaphase-promoting complex
molecular_function GO:0005515 protein binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.235740.1Cucsa.235740.1mRNA


Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001680WD40 repeatSMARTSM00320WD40_4coord: 28..66
score: 9.6E-4coord: 69..114
score:
IPR015943WD40/YVTN repeat-like-containing domainGENE3DG3DSA:2.130.10.10coord: 4..165
score: 3.0
IPR017986WD40-repeat-containing domainPROFILEPS50294WD_REPEATS_REGIONcoord: 34..95
score: 9
IPR017986WD40-repeat-containing domainunknownSSF50978WD40 repeat-likecoord: 5..166
score: 3.3
IPR024789Anaphase-promoting complex subunit 4PANTHERPTHR13260ANAPHASE PROMOTING COMPLEX SUBUNIT 4 APC4 CYCLOSOME SUBUNIT 4coord: 1..399
score: 1.5E
IPR024790Anaphase-promoting complex subunit 4 long domainPFAMPF12896ANAPC4coord: 215..411
score: 9.6
IPR024977Anaphase-promoting complex subunit 4, WD40 domainPFAMPF12894ANAPC4_WD40coord: 5..90
score: 7.5

The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cucsa.235740MELO3C025319Melon (DHL92) v3.5.1cgymeB396
The following gene(s) are paralogous to this gene:

None