Cucsa.235640 (gene) Cucumber (Gy14) v1

NameCucsa.235640
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionRetrovirus-related Pol polyprotein from transposon TNT 1-94
Locationscaffold02012 : 217399 .. 220565 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AAGTATTGTCGATGCTGATGTTGTAAATCTTGCCACACAACAGAGCAGTTGGGTGATTGATAGTGGTGCATCAGTTCATGCTACTTCGAAGAGGGAATTTTTTGCATCCTATACTTCTGGTGATTTTGGCAGTGTTAGGATGGGTAATGACAAATCAGCAAATACAATTGGCATCATAGATGTACACTTGAAGAATGAAAATGGTTCTAGGCTGATTTTGAAAAACGTGAAACATATTCCTTATATTCGCATGAACTTGATTTCTACAGGTAAGCTTGATGACAAGGGTTTCTGCAATACCTTTGACAATGGCATATGGAAGCTTACTAAAGGTTCAATGGATATAGCAAAGGGACAAAAATTATCTTCACTGTACTACATGGATGCAAAAATCATAGATTTTGATATAAATACAATGAATGATGAAGCAAATGTTAAGCTTTGGCATAAGAGACTTAGTCATATAAGTGAGAAGGGTTTAAAGATTTTAACAAAAAAAAATCATCTTCCTGATCCAAAGAGTACACCTCTAAAACGGTGTCCTCATTGTCTGGCAGGAAAGCAGACAAGAGTTACCTTTAAATCATCCCAGCATTCAAGGAAGCCAAATGTACTAGAGTTGGTATATTCTGATGTGTGTGGTCCCATGAAAACAAAGTCTCTTGGGGGTGCTTTATATTTTATGACATTTACTGATGATCATTCAAGGAAAATATGAGTTTACACCTTGAAGACTAAAGATCAAGTGTTGCAAGCATTTAAACAGTTTCATGCCTTCGTTGAGAAAGAAACTTGTGAAAATCTCAAGTGTGTTAGAACTGATAATGGAGGTGAGTATTATGGACCTTTTGATGAATATTGTAGAAATCATGACATTCGACATTAAAAGACACCTCCTAAGACACCGTAGTTAAATGGGATAGCTGAAAAATTGAACAGAACATTGGTTGAGAGAGTGAGATGCTTATTATTTGAATCACAGTTGCCACAGTCCTTCTAAGGCAAAACTTTAAATACAGTTGTACATGTTTTGAATTTCACACCATGTGTTCCATTGGGATCGAAAGTTCCAAACAGAATATGGTCAGGTAAGGATATATCTTATAGTCACCTATGTGTCTTTGGTTGTAAAGCTTTTGTTCATGTACCTAAAGATGACATATCAAAGTTTGATGCAAAAACTAAAGCATGTGTGTTTCTTGGTTATGGCCAAGATGAGTTTGGTTATAAATTATATGATCCAGTTAAGAAAAAACTTATAAGAAGTCGAGATGTTGTATTTGTTGAAGACCAAACAATAGCAGACATTGAGAAAATAGCTAAACATAGTGATAATCTAATTGATTTGAGTTCAACCTCTTTGACACAGTCTTCTACACAAATAGAAGATGAGGTTCAAAATAGACAACTTTTTTTACACAAGTGAGAGTTCTGAGCAGGTTTGGACAGAGGATAGAGTTGAAGAACAGTTAGCTGAAACAATTGTTCCTACAGATGTTTCACTCAGGAGATCTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGAAATATGAAGAACATACCTCAAAGACATGTTAAAAAGCAAGATTGGTTGTCAAAGGGTTCAATCAGAAGAAAGGTATCGACTTTGATGAAATTTTTGCTCCAGTTGTCAAGATGTCCTCCATACGTGTTGTTCTGGGTTTGGCAGCCAGTCTTGTCTTAGAGGTTGAGCAGATGGACGTTAAGACTGCTTTTCTTCATGGGGATTTAGACAAAGAAATCTATATGAGAACAACCAGAGGATTTTGAGTAGAAAGGAAAAGAGCATCTGGTGTGTAAATTGAAGAAAAGTCTCTATGGGTTGAAACAGGCACCGAGAAAGTGGTACAAGAAGTTTGAGTCAATTATGGGGGAGCAAGGCTATAGAAAAACTACTTCTGATCACTATGTTTTTTTCCATAAATTTTTTGATGATGATTTTGTTATATTATTACTCTATGATGATGATGATTTTGATTGTTGGCAGGAATGTTTCGAGAATTGATAACTTGAAGAAACAATTGAGCAAATCCTTTGCCATGAAGGATTTGGGGCCAGCAAAGAAAGTTCTTGGAATTCAAATAGTTCAAGACAAAGCATCCAAGGAGTTATACATGTCACCAGAGCAATACATAGAAAGTACTTGAACGTTTCAAGATGAGTCAAGCAAAATCAGTTAGTTCTCCTTTACCCAGTCACTTCAAACTTATCAGTAAACAGAGCCCTTCTACATATAAAGAGAAGGAGGATATGAATAAGGTCCCATATGCTTCAGCAGTTGGAAGCCTAATGTAGTATGTCATGGTATGTACTAGACCTGATATTTCTCATGTTATTGGTGTTGTTAGTCGTTATATGTATAATCCAGGAAAACAACATTGGGAGGCAGTCAAGTGCATCATGAGATATTTGAGAGGTACTTCCAGTTTGAAGCTCACATTTGGGAATGGAAAGCCAATACTTGCAGGTATAATGATTCAGATATGGCAGGGGACTTAGACAGCAAAAGTCTACTTCTGGTTACTTGATGACATTTGCAGGTGGTGCAGTGTTTTGGCAGTCAAGGTTGCAGAAATGTGTTGCCCTTTCTACAACTGAAGCAGAGTATATTGCAGTAGTAGAAACATGTAAAGAGATGTTGTGGATGAAGCGCTTTGTACAAAAGCTTGGCTTCAAGCAACAACGATATGTGATATATTGTGACAATATATTGATAAGAATGCTTTCCTTTGTACAAAAGCTTGGTAAGAATGCTTCATTTCATTCCAGAACAAAGCATATTGATGTGAGATATCAATGGCTTAAAGATGCTTTAAATGATGAATTGTTTGAGCTTGAGAAGATACACACTAATCATAATGAATTTGATATGTTGACAAAGAATCTACCAAGATCGAAGCTTGAGTTTTGTCGTTCCACAGTGGGAATGACAAATTCATCCTCAAAATGA

mRNA sequence

aagtattgtcgatgctgatgttgtaaatcttgccacacaacagagcagttgggtgattgatagtggtgcatcagttcatgctacttcgaagagggaattttttgcatcctatacttctggtgattttggcagtgttaggatgggtaatgacaaatcagcaaatacaattggcatcatagatgtacacttgaagaatgaaaatggttctaggctgattttgaaaaacgtgaaacatattccttatattcgcatgaacttgatttctacaggtaagcttgatgacaagggtttctgcaatacctttgacaatggcatatggaagcttactaaaggttcaatggatatagcaaagggacaaaaattatcttcactgtactacatggatgcaaaaatcatagattttgatataaatacaatgaatgatgaagcaaatgttaagctttggcataagagacttagtcatataagtgagaagggtttaaagattttaacaaaaaaaaatcatcttcctgatccaaagagtacacctctaaaacggtgtcctcattgtctggcaggaaagcagacaagagttacctttaaatcatcccagcattcaaggaagccaaatgtactagagttggtatattctgatgtgtgtggtcccatgaaaacaaagtctcttggggtttacaccttgaagactaaagatcaagtgttgcaagcatttaaacagtttcatgccttcgttgagaaagaaacttgtgaaaatctcaagtgtgttagaactgataatggaggcaaaactttaaatacagttgtacatgttttgaatttcacaccatgtgttccattgggatcgaaagttccaaacagaatatggtcaggtaaggatatatcttatagtcacctatgtgtctttggttgtaaagcttttgttcatgtacctaaagatgacatatcaaagtttgatgcaaaaactaaagcatgtgtgtttcttggttatggccaagatgagtttggttataaattatatgatccagttaagaaaaaacttataagaagtcgagatgttgtatttgttgaagaccaaacaatagcagacattgagaaaatagctaaacatagtgataatctaattgatttgagttcaacctctttgacacagtcttctacacaaatagaagatgaggttcaaaatagacaactttttttacacaaattggttgtcaaagggttcaatcagaagaaaggtatcgactttgatgaaatttttgctccagttgtcaagatgtcctccatacgtgttgttctgggtttggcagccagtcttgtcttagaggttgagcagatggacaaaggaaaagagcatctggtgtgtaaattgaagaaaagtctctatgggttgaaacaggcaccgagaaagtggtacaagaagtttgaGTCAATTATGGGGGAGCAAGGCTATAGAAAAACTACTTCTGATCACTATGTTTTTTTCCATAAATTTTTTGATGATGATTTTaaagtacttgaacgtttcaagatgagtcaagcaaaatcagttagttctcctttacccagtcacttcaaacttatcagtaaacagagcccttctacatataaagagaaggaggatatgaataagtatgtcatggtatgtactagacctgatatttctcatgttattggtgttgttagtcgttatatgtataatccaggaaaacaacattgggaggcagtcaagtgcatcatgagatatttgagaggtacttccagtttgaagctcacatttgggaatggaaagccaatacttgcagGTGGTGCAGTGTTTTGGCAGTCAAGGTTGCAGAAATGTGTTGCCCTTTCTACAACTGAAGCAGAGTATATTGCAGTAGTAGAAACATGTAAAGAGATGTTGTGGATGAAGCGCTTTGTACAAAAGCTTGGCTTCAAGCAACAACGATATGTGATATATTGTGACAATATATTGATAAGAATGCTTTCCTTTGTACAAAAGCTTGGTAAGAATGCTTCATTTCATTCCAGAACAAAGCATATTGATGTGAGATATCAATGGCTTAAAGATGCTTTAAATGATGAATTGTTTGAGCTTGAGAAGATACACACTAATCATAATGAATTTGATATGTTGACAAAGAATCTACCAAGATCGAAGCTTGAGTTTTGTCGTTCCACAGTGGGAATGACAAATTCATCCTCAAAATGA

Coding sequence (CDS)

AAGTATTGTCGATGCTGATGTTGTAAATCTTGCCACACAACAGAGCAGTTGGGTGATTGATAGTGGTGCATCAGTTCATGCTACTTCGAAGAGGGAATTTTTTGCATCCTATACTTCTGGTGATTTTGGCAGTGTTAGGATGGGTAATGACAAATCAGCAAATACAATTGGCATCATAGATGTACACTTGAAGAATGAAAATGGTTCTAGGCTGATTTTGAAAAACGTGAAACATATTCCTTATATTCGCATGAACTTGATTTCTACAGGTAAGCTTGATGACAAGGGTTTCTGCAATACCTTTGACAATGGCATATGGAAGCTTACTAAAGGTTCAATGGATATAGCAAAGGGACAAAAATTATCTTCACTGTACTACATGGATGCAAAAATCATAGATTTTGATATAAATACAATGAATGATGAAGCAAATGTTAAGCTTTGGCATAAGAGACTTAGTCATATAAGTGAGAAGGGTTTAAAGATTTTAACAAAAAAAAATCATCTTCCTGATCCAAAGAGTACACCTCTAAAACGGTGTCCTCATTGTCTGGCAGGAAAGCAGACAAGAGTTACCTTTAAATCATCCCAGCATTCAAGGAAGCCAAATGTACTAGAGTTGGTATATTCTGATGTGTGTGGTCCCATGAAAACAAAGTCTCTTGGGGTTTACACCTTGAAGACTAAAGATCAAGTGTTGCAAGCATTTAAACAGTTTCATGCCTTCGTTGAGAAAGAAACTTGTGAAAATCTCAAGTGTGTTAGAACTGATAATGGAGGCAAAACTTTAAATACAGTTGTACATGTTTTGAATTTCACACCATGTGTTCCATTGGGATCGAAAGTTCCAAACAGAATATGGTCAGGTAAGGATATATCTTATAGTCACCTATGTGTCTTTGGTTGTAAAGCTTTTGTTCATGTACCTAAAGATGACATATCAAAGTTTGATGCAAAAACTAAAGCATGTGTGTTTCTTGGTTATGGCCAAGATGAGTTTGGTTATAAATTATATGATCCAGTTAAGAAAAAACTTATAAGAAGTCGAGATGTTGTATTTGTTGAAGACCAAACAATAGCAGACATTGAGAAAATAGCTAAACATAGTGATAATCTAATTGATTTGAGTTCAACCTCTTTGACACAGTCTTCTACACAAATAGAAGATGAGGTTCAAAATAGACAACTTTTTTTACACAAATTGGTTGTCAAAGGGTTCAATCAGAAGAAAGGTATCGACTTTGATGAAATTTTTGCTCCAGTTGTCAAGATGTCCTCCATACGTGTTGTTCTGGGTTTGGCAGCCAGTCTTGTCTTAGAGGTTGAGCAGATGGACAAAGGAAAAGAGCATCTGGTGTGTAAATTGAAGAAAAGTCTCTATGGGTTGAAACAGGCACCGAGAAAGTGGTACAAGAAGTTTGAGTCAATTATGGGGGAGCAAGGCTATAGAAAAACTACTTCTGATCACTATGTTTTTTTCCATAAATTTTTTGATGATGATTTTAAAGTACTTGAACGTTTCAAGATGAGTCAAGCAAAATCAGTTAGTTCTCCTTTACCCAGTCACTTCAAACTTATCAGTAAACAGAGCCCTTCTACATATAAAGAGAAGGAGGATATGAATAAGTATGTCATGGTATGTACTAGACCTGATATTTCTCATGTTATTGGTGTTGTTAGTCGTTATATGTATAATCCAGGAAAACAACATTGGGAGGCAGTCAAGTGCATCATGAGATATTTGAGAGGTACTTCCAGTTTGAAGCTCACATTTGGGAATGGAAAGCCAATACTTGCAGGTGGTGCAGTGTTTTGGCAGTCAAGGTTGCAGAAATGTGTTGCCCTTTCTACAACTGAAGCAGAGTATATTGCAGTAGTAGAAACATGTAAAGAGATGTTGTGGATGAAGCGCTTTGTACAAAAGCTTGGCTTCAAGCAACAACGATATGTGATATATTGTGACAATATATTGATAAGAATGCTTTCCTTTGTACAAAAGCTTGGTAAGAATGCTTCATTTCATTCCAGAACAAAGCATATTGATGTGAGATATCAATGGCTTAAAGATGCTTTAAATGATGAATTGTTTGAGCTTGAGAAGATACACACTAATCATAATGAATTTGATATGTTGACAAAGAATCTACCAAGATCGAAGCTTGAGTTTTGTCGTTCCACAGTGGGAATGACAAATTCATCCTCAAAATGA

Protein sequence

SIVDADVVNLATQQSSWVIDSGASVHATSKREFFASYTSGDFGSVRMGNDKSANTIGIIDVHLKNENGSRLILKNVKHIPYIRMNLISTGKLDDKGFCNTFDNGIWKLTKGSMDIAKGQKLSSLYYMDAKIIDFDINTMNDEANVKLWHKRLSHISEKGLKILTKKNHLPDPKSTPLKRCPHCLAGKQTRVTFKSSQHSRKPNVLELVYSDVCGPMKTKSLGVYTLKTKDQVLQAFKQFHAFVEKETCENLKCVRTDNGGKTLNTVVHVLNFTPCVPLGSKVPNRIWSGKDISYSHLCVFGCKAFVHVPKDDISKFDAKTKACVFLGYGQDEFGYKLYDPVKKKLIRSRDVVFVEDQTIADIEKIAKHSDNLIDLSSTSLTQSSTQIEDEVQNRQLFLHKLVVKGFNQKKGIDFDEIFAPVVKMSSIRVVLGLAASLVLEVEQMDKGKEHLVCKLKKSLYGLKQAPRKWYKKFESIMGEQGYRKTTSDHYVFFHKFFDDDFKVLERFKMSQAKSVSSPLPSHFKLISKQSPSTYKEKEDMNKYVMVCTRPDISHVIGVVSRYMYNPGKQHWEAVKCIMRYLRGTSSLKLTFGNGKPILAGGAVFWQSRLQKCVALSTTEAEYIAVVETCKEMLWMKRFVQKLGFKQQRYVIYCDNILIRMLSFVQKLGKNASFHSRTKHIDVRYQWLKDALNDELFELEKIHTNHNEFDMLTKNLPRSKLEFCRSTVGMTNSSSK*
BLAST of Cucsa.235640 vs. Swiss-Prot
Match: POLX_TOBAC (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 5.4e-69
Identity = 154/335 (45.97%), Postives = 200/335 (59.70%), Query Frame = 1

Query: 437  LVLEVEQM-----DKGKEHLVCKLKKSLYGLKQAPRKWYKKFESIMGEQGYRKTTSDH-Y 496
            L+L V+ M     DKG   L+ KLK  L   K    K     + I+G +  R+ TS   +
Sbjct: 1005 LLLYVDDMLIVGKDKG---LIAKLKGDLS--KSFDMKDLGPAQQILGMKIVRERTSRKLW 1064

Query: 497  VFFHKFFDDDFKVLERFKMSQAKSVSSPLPSHFKLISKQSPSTYKEKEDMNK-------- 556
            +   K+ +   +VLERF M  AK VS+PL  H KL  K  P+T +EK +M K        
Sbjct: 1065 LSQEKYIE---RVLERFNMKNAKPVSTPLAGHLKLSKKMCPTTVEEKGNMAKVPYSSAVG 1124

Query: 557  ---YVMVCTRPDISHVIGVVSRYMYNPGKQHWEAVKCIMRYLRGTSSLKLTFGNGKPIL- 616
               Y MVCTRPDI+H +GVVSR++ NPGK+HWEAVK I+RYLRGT+   L FG   PIL 
Sbjct: 1125 SLMYAMVCTRPDIAHAVGVVSRFLENPGKEHWEAVKWILRYLRGTTGDCLCFGGSDPILK 1184

Query: 617  ------------------------AGGAVFWQSRLQKCVALSTTEAEYIAVVETCKEMLW 676
                                    +GGA+ WQS+LQKCVALSTTEAEYIA  ET KEM+W
Sbjct: 1185 GYTDADMAGDIDNRKSSTGYLFTFSGGAISWQSKLQKCVALSTTEAEYIAATETGKEMIW 1244

Query: 677  MKRFVQKLGFKQQRYVIYCDNILIRMLSFVQKLGKNASFHSRTKHIDVRYQWLKDALNDE 730
            +KRF+Q+LG  Q+ YV+YCD+           L KN+ +H+RTKHIDVRY W+++ ++DE
Sbjct: 1245 LKRFLQELGLHQKEYVVYCDS------QSAIDLSKNSMYHARTKHIDVRYHWIREMVDDE 1304


HSP 2 Score: 250.8 bits (639), Expect = 4.8e-65
Identity = 130/275 (47.27%), Postives = 181/275 (65.82%), Query Frame = 1

Query: 4   DADVVNLATQQSSWVIDSGASVHATSKREFFASYTSGDFGSVRMGNDKSANTIGIIDVHL 63
           + + ++L+  +S WV+D+ AS HAT  R+ F  Y +GDFG+V+MGN   +   GI D+ +
Sbjct: 281 EEECMHLSGPESEWVVDTAASHHATPVRDLFCRYVAGDFGTVKMGNTSYSKIAGIGDICI 340

Query: 64  KNENGSRLILKNVKHIPYIRMNLISTGKLDDKGFCNTFDNGIWKLTKGSMDIAKGQKLSS 123
           K   G  L+LK+V+H+P +RMNLIS   LD  G+ + F N  W+LTKGS+ IAKG    +
Sbjct: 341 KTNVGCTLVLKDVRHVPDLRMNLISGIALDRDGYESYFANQKWRLTKGSLVIAKGVARGT 400

Query: 124 LYYMDAKIIDFDINTMNDEANVKLWHKRLSHISEKGLKILTKKNHLPDPKSTPLKRCPHC 183
           LY  +A+I   ++N   DE +V LWHKR+ H+SEKGL+IL KK+ +   K T +K C +C
Sbjct: 401 LYRTNAEICQGELNAAQDEISVDLWHKRMGHMSEKGLQILAKKSLISYAKGTTVKPCDYC 460

Query: 184 LAGKQTRVTFKSSQHSRKPNVLELVYSDVCGPMKTKSLG-----------------VYTL 243
           L GKQ RV+F++S   RK N+L+LVYSDVCGPM+ +S+G                 VY L
Sbjct: 461 LFGKQHRVSFQTSS-ERKLNILDLVYSDVCGPMEIESMGGNKYFVTFIDDASRKLWVYIL 520

Query: 244 KTKDQVLQAFKQFHAFVEKETCENLKCVRTDNGGK 262
           KTKDQV Q F++FHA VE+ET   LK +R+DNGG+
Sbjct: 521 KTKDQVFQVFQKFHALVERETGRKLKRLRSDNGGE 554


HSP 3 Score: 138.3 bits (347), Expect = 3.4e-31
Identity = 68/159 (42.77%), Postives = 106/159 (66.67%), Query Frame = 1

Query: 260 GKTLNTVVHVLNFTPCVPLGSKVPNRIWSGKDISYSHLCVFGCKAFVHVPKDDISKFDAK 319
           G+ + T  +++N +P VPL  ++P R+W+ K++SYSHL VFGC+AF HVPK+  +K D K
Sbjct: 611 GEAVQTACYLINRSPSVPLAFEIPERVWTNKEVSYSHLKVFGCRAFAHVPKEQRTKLDDK 670

Query: 320 TKACVFLGYGQDEFGYKLYDPVKKKLIRSRDVVFVEDQ--TIADIEKIAKHS--DNLIDL 379
           +  C+F+GYG +EFGY+L+DPVKKK+IRSRDVVF E +  T AD+ +  K+    N + +
Sbjct: 671 SIPCIFIGYGDEEFGYRLWDPVKKKVIRSRDVVFRESEVRTAADMSEKVKNGIIPNFVTI 730

Query: 380 SSTSLTQSSTQ-IEDEVQNRQLFLHKLVVKGFNQKKGID 414
            STS   +S +   DEV  +     +++ +G    +G++
Sbjct: 731 PSTSNNPTSAESTTDEVSEQGEQPGEVIEQGEQLDEGVE 769


HSP 4 Score: 135.6 bits (340), Expect = 2.2e-30
Identity = 72/129 (55.81%), Postives = 87/129 (67.44%), Query Frame = 1

Query: 400  KLVVKGFNQKKGIDFDEIFAPVVKMSSIRVVLGLAASLVLEVEQMD-------------- 459
            +LVVKGF QKKGIDFDEIF+PVVKM+SIR +L LAASL LEVEQ+D              
Sbjct: 877  RLVVKGFEQKKGIDFDEIFSPVVKMTSIRTILSLAASLDLEVEQLDVKTAFLHGDLEEEI 936

Query: 460  ----------KGKEHLVCKLKKSLYGLKQAPRKWYKKFESIMGEQGYRKTTSDHYVFFHK 505
                       GK+H+VCKL KSLYGLKQAPR+WY KF+S M  Q Y KT SD  V+F +
Sbjct: 937  YMEQPEGFEVAGKKHMVCKLNKSLYGLKQAPRQWYMKFDSFMKSQTYLKTYSDPCVYFKR 996

BLAST of Cucsa.235640 vs. Swiss-Prot
Match: COPIA_DROME (Copia protein OS=Drosophila melanogaster GN=GIP PE=1 SV=3)

HSP 1 Score: 118.2 bits (295), Expect = 3.7e-25
Identity = 75/262 (28.63%), Postives = 127/262 (48.47%), Query Frame = 1

Query: 502  KVLERFKMSQAKSVSSPLPS--HFKLISKQSPSTYKEKEDMN--KYVMVCTRPDISHVIG 561
            K+L +F M    +VS+PLPS  +++L++         +  +    Y+M+CTRPD++  + 
Sbjct: 1145 KILSKFNMENCNAVSTPLPSKINYELLNSDEDCNTPCRSLIGCLMYIMLCTRPDLTTAVN 1204

Query: 562  VVSRYMYNPGKQHWEAVKCIMRYLRGTSSLKLTF-----------GNGKPILAGGAV--- 621
            ++SRY      + W+ +K ++RYL+GT  +KL F           G      AG  +   
Sbjct: 1205 ILSRYSSKNNSELWQNLKRVLRYLKGTIDMKLIFKKNLAFENKIIGYVDSDWAGSEIDRK 1264

Query: 622  ---------------FWQSRLQKCVALSTTEAEYIAVVETCKEMLWMKRFVQKLGFKQQR 681
                            W ++ Q  VA S+TEAEY+A+ E  +E LW+K  +  +  K + 
Sbjct: 1265 STTGYLFKMFDFNLICWNTKRQNSVAASSTEAEYMALFEAVREALWLKFLLTSINIKLEN 1324

Query: 682  YV-IYCDNILIRMLSFVQKLGKNASFHSRTKHIDVRYQWLKDALNDELFELEKIHTNHNE 730
             + IY DN           +  N S H R KHID++Y + ++ + + +  LE I T +  
Sbjct: 1325 PIKIYEDN------QGCISIANNPSCHKRAKHIDIKYHFAREQVQNNVICLEYIPTENQL 1384


HSP 2 Score: 73.2 bits (178), Expect = 1.4e-11
Identity = 44/126 (34.92%), Postives = 66/126 (52.38%), Query Frame = 1

Query: 389  DEVQNRQLFLHKLVVKGFNQKKGIDFDEIFAPVVKMSSIRVVLGLAASLVLEVEQMDKG- 448
            +E+ N   +  +LV +GF QK  ID++E FAPV ++SS R +L L     L+V QMD   
Sbjct: 946  NELGNPIRYKARLVARGFTQKYQIDYEETFAPVARISSFRFILSLVIQYNLKVHQMDVKT 1005

Query: 449  -------KEHL--------------VCKLKKSLYGLKQAPRKWYKKFESIMGEQGYRKTT 493
                   KE +              VCKL K++YGLKQA R W++ FE  + E  +  ++
Sbjct: 1006 AFLNGTLKEEIYMRLPQGISCNSDNVCKLNKAIYGLKQAARCWFEVFEQALKECEFVNSS 1065


HSP 3 Score: 61.2 bits (147), Expect = 5.4e-08
Identity = 37/102 (36.27%), Postives = 55/102 (53.92%), Query Frame = 1

Query: 260 GKTLNTVVHVLNFTPCVPL--GSKVPNRIWSGKDISYSHLCVFGCKAFVHVPKDDISKFD 319
           G+ + T  +++N  P   L   SK P  +W  K     HL VFG   +VH+ K+   KFD
Sbjct: 611 GEAVLTATYLINRIPSRALVDSSKTPYEMWHNKKPYLKHLRVFGATVYVHI-KNKQGKFD 670

Query: 320 AKTKACVFLGYGQDEFGYKLYDPVKKKLIRSRDVVFVEDQTI 360
            K+   +F+GY  +  G+KL+D V +K I +RDVV  E   +
Sbjct: 671 DKSFKSIFVGY--EPNGFKLWDAVNEKFIVARDVVVDETNMV 709

BLAST of Cucsa.235640 vs. Swiss-Prot
Match: M300_ARATH (Uncharacterized mitochondrial protein AtMg00300 OS=Arabidopsis thaliana GN=AtMg00300 PE=4 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 2.2e-14
Identity = 45/114 (39.47%), Postives = 66/114 (57.89%), Query Frame = 1

Query: 104 GIWKLTKGSMDIAKGQKLSSLYYMDAKIIDFDIN---TMNDEANVKLWHKRLSHISEKGL 163
           G+ K+ KG   I KG +  SLY +   +   + N   T  DE   +LWH RL+H+S++G+
Sbjct: 27  GVLKVLKGCRTILKGNRHDSLYILQGSVETGESNLAETAKDET--RLWHSRLAHMSQRGM 86

Query: 164 KILTKKNHLPDPKSTPLKRCPHCLAGKQTRVTFKSSQHSRKPNVLELVYSDVCG 215
           ++L KK  L   K + LK C  C+ GK  RV F + QH+ K N L+ V+SD+ G
Sbjct: 87  ELLVKKGFLDSSKVSSLKFCEDCIYGKTHRVNFSTGQHTTK-NPLDYVHSDLWG 137

BLAST of Cucsa.235640 vs. Swiss-Prot
Match: M810_ARATH (Uncharacterized mitochondrial protein AtMg00810 OS=Arabidopsis thaliana GN=AtMg00810 PE=4 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 2.0e-07
Identity = 42/164 (25.61%), Postives = 71/164 (43.29%), Query Frame = 1

Query: 502 KVLERFKMSQAKSVSSPLPSHFKLISKQSPSTYKEKEDMNKYV-----MVCTRPDISHVI 561
           ++L    M   K +S+PLP   KL S  S + Y +  D    V     +  TRPDIS+ +
Sbjct: 64  QILNNAGMLDCKPMSTPLP--LKLNSSVSTAKYPDPSDFRSIVGALQYLTLTRPDISYAV 123

Query: 562 GVVSRYMYNPGKQHWEAVKCIMRYLRGT--------------------------SSLKLT 621
            +V + M+ P    ++ +K ++RY++GT                          +S + +
Sbjct: 124 NIVCQRMHEPTLADFDLLKRVLRYVKGTIFHGLYIHKNSKLNVQAFCDSDWAGCTSTRRS 183

Query: 622 FGNGKPILAGGAVFWQSRLQKCVALSTTEAEYIAVVETCKEMLW 635
                  L    + W ++ Q  V+ S+TE EY A+  T  E+ W
Sbjct: 184 TTGFCTFLGCNIISWSAKRQPTVSRSSTETEYRALALTAAELTW 225

BLAST of Cucsa.235640 vs. TrEMBL
Match: A5C9D7_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_007304 PE=4 SV=1)

HSP 1 Score: 503.1 bits (1294), Expect = 5.9e-139
Identity = 264/472 (55.93%), Postives = 310/472 (65.68%), Query Frame = 1

Query: 2   IVDADVVNLATQQSSWVIDSGASVHATSKREFFASYTSGDFGSVRMGNDKSANTIGIIDV 61
           + D+DVVN A Q+S WVID GAS+HAT +++FF SYTSGDFGSVRMGND SA  IG+ DV
Sbjct: 256 VYDSDVVNFACQESXWVIDXGASIHATPQKDFFTSYTSGDFGSVRMGNDGSAKAIGMGDV 315

Query: 62  HLKNENGSRLILKNVKHIPYIRMNLISTGKLDDKGFCNTFDNGIWKLTKGSMDIAKGQKL 121
            L+  NG+ L LKNVKHIP IRMNLISTGKLDD+GFCNTF +  WKLT+GSM IAKG K 
Sbjct: 316 RLETSNGTMLTLKNVKHIPDIRMNLISTGKLDDEGFCNTFRDSQWKLTRGSMVIAKGNKS 375

Query: 122 SSLYYMDAKIIDFDINTMNDEANVKLWHKRLSHISEKGLKILTKKNHLPDPKSTPLKRCP 181
           SSLY M A++ID  IN ++D++  +LWH +L H+SEKGL IL KKN L   K   LKRC 
Sbjct: 376 SSLYLMQARVIDSSINAVDDDSTFELWHNKLGHMSEKGLMILAKKNLLXGMKKGSLKRCA 435

Query: 182 HCLAGKQTRVTFKSSQHSRKPNVLELVYSDVCGPMKTKSLG-----------------VY 241
           HCLAGKQTRV FK+  H+RKP +L+LVYSDV GPMKTK+LG                 VY
Sbjct: 436 HCLAGKQTRVAFKTLHHTRKPGMLDLVYSDVYGPMKTKTLGGSLYFVTFIDDHSRKIWVY 495

Query: 242 TLKTKDQVLQAFKQFHAFVEKETCENLKCVRTDNG------------------------- 301
           TLKTKDQVL  FKQFHA VE+++ E LKC+RTDNG                         
Sbjct: 496 TLKTKDQVLDVFKQFHALVERQSGEKLKCIRTDNGGEYSGPFDEYCRQHGFRHQKTPPKT 555

Query: 302 --------------------------------GKTLNTVVHVLNFTPCVPLGSKVPNRIW 361
                                           G+ LNT+VHVLN TPCVPL   VP+RIW
Sbjct: 556 PQLNGLAERMNRTLVERVRCLLSQSQLPRSFWGEALNTIVHVLNLTPCVPLEFDVPDRIW 615

Query: 362 SGKDISYSHLCVFGCKAFVHVPKDDISKFDAKTKACVFLGYGQDEFGYKLYDPVKKKLIR 396
           S  +ISY HL VFGCKAFVH+PKD+ SK DAKT+ CVF+GYGQDE GYK YD V+KKL R
Sbjct: 616 SNNEISYDHLRVFGCKAFVHIPKDERSKLDAKTRPCVFIGYGQDELGYKFYDLVQKKLXR 675

BLAST of Cucsa.235640 vs. TrEMBL
Match: A5C9D7_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_007304 PE=4 SV=1)

HSP 1 Score: 311.2 bits (796), Expect = 3.3e-81
Identity = 161/270 (59.63%), Postives = 189/270 (70.00%), Query Frame = 1

Query: 502  KVLERFKMSQAKSVSSPLPSHFKLISKQSPSTYKEKEDMNK-----------YVMVCTRP 561
            KV  RF MS+ K VSSPL SHFKL S+ SPST KEKEDM +           Y MVCTRP
Sbjct: 1039 KVFARFNMSKFKVVSSPLASHFKLSSRHSPSTDKEKEDMRRVPYASAIGSLMYAMVCTRP 1098

Query: 562  DISHVIGVVSRYMYNPGKQHWEAVKCIMRYLRGTSSLKLTFGNGKPIL------------ 621
            DI++ +GVVSR++ NPG+ HWEAVK IMRYLRGTS LKLTFG+GKPIL            
Sbjct: 1099 DIAYAVGVVSRFLSNPGRHHWEAVKWIMRYLRGTSKLKLTFGSGKPILVGYTDSDMAGDV 1158

Query: 622  -------------AGGAVFWQSRLQKCVALSTTEAEYIAVVETCKEMLWMKRFVQKLGFK 681
                         +GGAV WQSRLQKCVALSTTEAEYIA VE CKE+LWMK F+Q+LGFK
Sbjct: 1159 DNRKSTSGYLMTFSGGAVSWQSRLQKCVALSTTEAEYIAAVEACKELLWMKCFMQELGFK 1218

Query: 682  QQRYVIYCDNILIRMLSFVQKLGKNASFHSRTKHIDVRYQWLKDALNDELFELEKIHTNH 736
            QQRYV+YCDN           L KN+++H+R+KHIDVRY W++DALND LFE+EKIHT++
Sbjct: 1219 QQRYVVYCDN------QSAIHLSKNSTYHARSKHIDVRYHWMRDALNDNLFEIEKIHTDN 1278


HSP 2 Score: 109.4 bits (272), Expect = 1.9e-20
Identity = 61/117 (52.14%), Postives = 75/117 (64.10%), Query Frame = 1

Query: 388 EDEVQNRQLFLHKLVVKGFNQKKGIDFDEIFAPVVKMSSIRVVLGLAASLVLEVEQMDKG 447
           ++E  ++  +  +LVVK    K+  D  EI    VK + +   L     +      + KG
Sbjct: 861 QEEHTSQPRYKARLVVKRVQSKESFDL-EIQQMDVKTAFLHGDLDKEIYMEQPEGFVLKG 920

Query: 448 KEHLVCKLKKSLYGLKQAPRKWYKKFESIMGEQGYRKTTSDHYVFFHKFFDDDFKVL 505
           KE  VCKLKKSLYGLKQAPR+WYKKFES+MGEQGYRKTTSDH VF  KF DDDF +L
Sbjct: 921 KEDYVCKLKKSLYGLKQAPRQWYKKFESVMGEQGYRKTTSDHCVFVQKFSDDDFVIL 976


HSP 3 Score: 470.7 bits (1210), Expect = 3.3e-129
Identity = 250/472 (52.97%), Postives = 302/472 (63.98%), Query Frame = 1

Query: 2   IVDADVVNLATQQSSWVIDSGASVHATSKREFFASYTSGDFGSVRMGNDKSANTIGIIDV 61
           + D+DVVN A Q++SWVIDSGAS+HAT +++FF SYT  DFGSVRMGND  A  IG+ DV
Sbjct: 30  VYDSDVVNFACQETSWVIDSGASIHATPRKDFFTSYTFSDFGSVRMGNDGLAKAIGMGDV 89

Query: 62  HLKNENGSRLILKNVKHIPYIRMNLISTGKLDDKGFCNTFDNGIWKLTKGSMDIAKGQKL 121
            L+  NG+ L LKNVKHIP IRMNLIST KLDD+G CNTF +  WKLT+GSM I K  K 
Sbjct: 90  RLETSNGTMLTLKNVKHIPDIRMNLISTRKLDDEGLCNTFRDSQWKLTRGSMVITKENKS 149

Query: 122 SSLYYMDAKIIDFDINTMNDEANVKLWHKRLSHISEKGLKILTKKNHLPDPKSTPLKRCP 181
           SSLY M  ++ID  IN ++D++  KLWH RLSH+SEKGL I+ KKN L   K   LKRC 
Sbjct: 150 SSLYLMQVRVIDSSINAVDDDSTFKLWHNRLSHMSEKGLMIMAKKNLLSGMKKGSLKRCA 209

Query: 182 HCLAGKQTRVTFKSSQHSRKPNVLELVYSDVCGPMKTKSLG-----------------VY 241
           HCL GKQTRV FK+  H+RKP +L+LVYSDVCGPMKTK+LG                 VY
Sbjct: 210 HCLGGKQTRVAFKTLHHTRKPGMLDLVYSDVCGPMKTKTLGGSLYFVTFIDDHSRKIWVY 269

Query: 242 TLKTKDQVLQAFKQFHAFVEKETCENLKCVRTDNGGK----------------------- 301
           TLKTKDQVL  FKQFHA VE+++ E LKC++ DNGG+                       
Sbjct: 270 TLKTKDQVLDVFKQFHALVERQSGEKLKCIQIDNGGEYSSPFDEYCRQHGIRHQKTPPKT 329

Query: 302 -TLNTVVHVLNFT-----PCV----------------------------PLGSKVPNRIW 361
             LN +   +N T      C+                            PL   VP+RIW
Sbjct: 330 PQLNGLAESMNRTLVERVRCLLSQSQLPRSFWGEALNTVVHLLNLTPCVPLEFDVPDRIW 389

Query: 362 SGKDISYSHLCVFGCKAFVHVPKDDISKFDAKTKACVFLGYGQDEFGYKLYDPVKKKLIR 396
           S  +I Y HL VFGCKAFVH+PKD+ISK DAKT+ CVF+GYG DE GY+ YDP++KKL+R
Sbjct: 390 SNNEICYDHLRVFGCKAFVHIPKDEISKLDAKTRPCVFIGYGHDELGYRFYDPMQKKLVR 449

BLAST of Cucsa.235640 vs. TrEMBL
Match: A5B9T5_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_030853 PE=4 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 8.7e-74
Identity = 181/391 (46.29%), Postives = 220/391 (56.27%), Query Frame = 1

Query: 388 EDEVQNRQLFLHKLVVKGFNQKKGIDFDEIFAPVVKMSSIRVVLGLAASLVLEVEQMDKG 447
           ++E  ++  +  +LVVKGFNQKK  +               + +      VL      KG
Sbjct: 615 QEEHTSQPRYKARLVVKGFNQKKDKE---------------IYMEQQEGFVL------KG 674

Query: 448 KEHLVCKLKKSLYGLKQAPR----------KWYKKF--------ESIMGEQGYRKTTSDH 507
           KE  V KLKKSLYGLKQAPR          +  K F        + I+G +  R   S  
Sbjct: 675 KEDYVSKLKKSLYGLKQAPRNVSRIDNLKKQLSKSFAMKDLGPVKRILGIRIERDRASKK 734

Query: 508 YVFFHKFFDDDFKVLERFKMSQAKSVSSPLPSHFKLISKQSPSTYKEKEDMNKYVMVCTR 567
                + + +  KVL RF MS+AK VSSPL SHFKL S+ SPST KEKEDM +       
Sbjct: 735 LCMLQEQYIE--KVLARFNMSKAKVVSSPLTSHFKLSSRHSPSTDKEKEDMRRVSYALAG 794

Query: 568 PDISHVIGVVSRYMYNPGKQHWEAVKCIMRYLRGTSSLKLTFGNGKPIL----------- 627
             + +V+               EAVK IMRYLRGTS LKLTFG GKPIL           
Sbjct: 795 GSLMYVM---------------EAVKWIMRYLRGTSKLKLTFGGGKPILVGYTDSDMAGD 854

Query: 628 --------------AGGAVFWQSRLQKCVALSTTEAEYIAVVETCKEMLWMKRFVQKLGF 687
                         +GGAV WQSRLQKCVALSTTEAEYIA  E CKE+LWMK F+Q+LGF
Sbjct: 855 VDNRRSTSGYLMTCSGGAVSWQSRLQKCVALSTTEAEYIAAAEACKELLWMKCFMQELGF 914

Query: 688 KQQRYVIYCDNILIRMLSFVQKLGKNASFHSRTKHIDVRYQWLKDALNDELFELEKIHTN 736
           KQQRYV+YCDN           L KN+++H+R+KHI VRY W++DALND L E+EKIHTN
Sbjct: 915 KQQRYVVYCDN------QSPIHLSKNSTYHARSKHIHVRYHWMRDALNDNLLEIEKIHTN 961


HSP 2 Score: 451.4 bits (1160), Expect = 2.0e-123
Identity = 235/415 (56.63%), Postives = 283/415 (68.19%), Query Frame = 1

Query: 2   IVDADVVNLATQQSSWVIDSGASVHATSKREFFASYTSGDFGSVRMGNDKSANTIGIIDV 61
           + D+DVVN A  ++SWVIDSGAS+HAT +++FF SYTS             A  IG+ DV
Sbjct: 241 VYDSDVVNFACXETSWVIDSGASIHATLRKDFFTSYTS-------------AKAIGMGDV 300

Query: 62  HLKNENGSRLILKNVKHIPYIRMNLISTGKLDDKGFCNTFDNGIWKLTKGSMDIAKGQKL 121
            L+  NG+ L LKNVKHIP IRMNLIST KLDD+GFCNTF +  W LT+GSM IAKG K 
Sbjct: 301 RLETSNGTMLTLKNVKHIPDIRMNLISTRKLDDEGFCNTFRDSQWNLTRGSMVIAKGNKS 360

Query: 122 SSLYYMDAKIIDFDINTMNDEANVKLWHKRLSHISEKGLKILTKKNHLPDPKSTPLKRCP 181
           SSLY M A++ID +IN ++D++  +LWH RL H+SEKGL IL KKN L   K   LKRC 
Sbjct: 361 SSLYLMQARVIDSNINAVDDDSTFELWHNRLGHMSEKGLMILAKKNLLSGMKKGSLKRCA 420

Query: 182 HCLAGKQTRVTFKSSQHSRKPNVLELVYSDVCGPMKTKSLG-----------------VY 241
           HCLAGKQTRV FK+  H+RKP +L+LVYSDVCGPMKTK+L                  VY
Sbjct: 421 HCLAGKQTRVAFKTLHHTRKPGMLDLVYSDVCGPMKTKTLSESLYFVTFIDDHSRKIWVY 480

Query: 242 TLKTKDQVLQAFKQFHAFVEKETCENLKCVRTDNGGKTLNTVVHVLNFTPCVPLGSKVPN 301
           TLKTKDQVL  FKQFHA VE+++ E LKC+R DNGG+             C      VP+
Sbjct: 481 TLKTKDQVLDVFKQFHALVERQSGEKLKCIRIDNGGEYSGPFDEY-----CRQXEFDVPD 540

Query: 302 RIWSGKDISYSHLCVFGCKAFVHVPKDDISKFDAKTKACVFLGYGQDEFGYKLYDPVKKK 361
           RIWS  +ISY HL VFGCKAFVH+PK + SK DAKT+ CVF+GY QDE GY+ YDPV+KK
Sbjct: 541 RIWSNNEISYDHLRVFGCKAFVHIPKYERSKLDAKTRPCVFIGYDQDELGYRFYDPVQKK 600

Query: 362 LIRSRDVVFVEDQTIADIEKI----AKHSDNLIDLSSTSLTQSSTQIEDEVQNRQ 396
           L+RSRDVV +ED TI DI K     ++HS +LIDL    LT   TQ+EDE  + Q
Sbjct: 601 LVRSRDVVXMEDHTIQDIXKTNPMESQHSXDLIDLDPAPLTNLPTQVEDEAHDDQ 637

BLAST of Cucsa.235640 vs. TrEMBL
Match: A5BAF2_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_027081 PE=4 SV=1)

HSP 1 Score: 304.3 bits (778), Expect = 4.0e-79
Identity = 158/270 (58.52%), Postives = 186/270 (68.89%), Query Frame = 1

Query: 502  KVLERFKMSQAKSVSSPLPSHFKLISKQSPSTYKEKEDMNK-----------YVMVCTRP 561
            K+L RF MS+AK VSSPL SHFKL S+ +PST KEKEDM +           YVMVCTRP
Sbjct: 885  KMLARFNMSKAKVVSSPLASHFKLSSRHNPSTDKEKEDMRRVSYASAVGSLMYVMVCTRP 944

Query: 562  DISHVIGVVSRYMYNPGKQHWEAVKCIMRYLRGTSSLKLTFGNGKPIL------------ 621
            DI++ IGVV R++ NPG+ HWEAVK IMRYLRGTS LKLTFG GKPIL            
Sbjct: 945  DIAYAIGVVIRFLSNPGRHHWEAVKWIMRYLRGTSKLKLTFGGGKPILVGYTDSDMAGDV 1004

Query: 622  -------------AGGAVFWQSRLQKCVALSTTEAEYIAVVETCKEMLWMKRFVQKLGFK 681
                         +GG V WQSR QKCVALSTTEAEYIA  E C E+LWMK F+Q+LGFK
Sbjct: 1005 DNRRSTLGYLMTFSGGTVSWQSRQQKCVALSTTEAEYIAAXEACNELLWMKCFMQELGFK 1064

Query: 682  QQRYVIYCDNILIRMLSFVQKLGKNASFHSRTKHIDVRYQWLKDALNDELFELEKIHTNH 736
            QQRYV+YCDN           L KN+++H+R+KHIDVRY W++DALND LFE+EKIHT++
Sbjct: 1065 QQRYVVYCDN------QSAIHLSKNSTYHARSKHIDVRYHWMRDALNDNLFEIEKIHTDN 1124


HSP 2 Score: 84.7 bits (208), Expect = 5.0e-13
Identity = 44/73 (60.27%), Postives = 55/73 (75.34%), Query Frame = 1

Query: 388 EDEVQNRQLFLHKLVVKGFNQKKGIDFDEIFAPVVKMSSIRVVLGLAASLVLEVEQMDKG 447
           ++E  ++  +  +L+VKGFNQKKG DFDEIF+PVVKMSSIRVVLGLAASL LE++QMD  
Sbjct: 733 QEEHTSQPXYKARLIVKGFNQKKGXDFDEIFSPVVKMSSIRVVLGLAASLDLEIQQMDVK 792

Query: 448 KEHLVCKLKKSLY 461
              L   L K +Y
Sbjct: 793 TAFLHGDLXKEIY 805


HSP 3 Score: 449.1 bits (1154), Expect = 1.0e-122
Identity = 240/442 (54.30%), Postives = 285/442 (64.48%), Query Frame = 1

Query: 32  EFFASYTSGDFGSVRMGNDKSANTIGIIDVHLKNENGSRLILKNVKHIPYIRMNLISTGK 91
           +FF SYTSGDFGSVRMGND SA  IG+ +V L+  NG+ L LKNVKHIP IRMNLISTGK
Sbjct: 189 DFFTSYTSGDFGSVRMGNDDSAKAIGMRNVRLETSNGTMLTLKNVKHIPDIRMNLISTGK 248

Query: 92  LDDKGFCNTFDNGIWKLTKGSMDIAKGQKLSSLYYMDAKIIDFDINTMNDEANVKLWHKR 151
           LDD+GF N F +  WKLT+GSM IAKG K SSLY M  ++ID  IN ++D++  +LWH R
Sbjct: 249 LDDEGFYNIFRDSQWKLTRGSMVIAKGNKSSSLYLMQTRVIDSSINAVDDDSTFELWHNR 308

Query: 152 LSHISEKGLKILTKKNHLPDPKSTPLKRCPHCLAGKQTRVTFKSSQHSRKPNVLELVYSD 211
           L H+SEKGL IL KKN L   K   LKRC HCLAGKQTRV FK+ +++RKP +L+LVYSD
Sbjct: 309 LGHMSEKGLMILAKKNLLSGMKKGSLKRCAHCLAGKQTRVAFKTLRYTRKPGMLDLVYSD 368

Query: 212 VCGPMKTKSLG-----------------VYTLKTKDQVLQAFKQFHAFVEKETCENLKCV 271
           VCGPMKTK+LG                 VYTLKTKDQVL  FKQFHA VE+++ E LKC+
Sbjct: 369 VCGPMKTKTLGGSLYFVTFIDDHSRKIWVYTLKTKDQVLDVFKQFHALVERQSGEKLKCI 428

Query: 272 RTDNGGK------------------------TLNTVVHVLNF------------------ 331
           RTDNGG+                         LN +   +N                   
Sbjct: 429 RTDNGGEYSSPFDEYCRQHGIRHQKTSPKTPQLNGLAERMNRTLVERVRCLLSQSQLPRS 488

Query: 332 ---------------TPCVPLGSKVPNRIWSGKDISYSHLCVFGCKAFVHVPKDDISKFD 391
                          TPCVPL   VP+RIWS  +ISY HL VFGCKAFVH+PKD+ SK D
Sbjct: 489 FWGEALNTVVHVLNLTPCVPLEFDVPDRIWSKNEISYDHLRVFGCKAFVHIPKDERSKLD 548

Query: 392 AKTKACVFLGYGQDEFGYKLYDPVKKKLIRSRDVVFVEDQTIADIEKI----AKHSDNLI 396
           AKT+ CVF+GYGQDE GY+ YDPV+KKL+RSRDVVF+ED TI DIEK     ++HS +LI
Sbjct: 549 AKTRPCVFIGYGQDELGYRFYDPVQKKLVRSRDVVFMEDHTIQDIEKTNPMESQHSGDLI 608

BLAST of Cucsa.235640 vs. TrEMBL
Match: A5C540_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_024474 PE=4 SV=1)

HSP 1 Score: 319.7 bits (818), Expect = 9.3e-84
Identity = 200/428 (46.73%), Postives = 237/428 (55.37%), Query Frame = 1

Query: 388  EDEVQNRQLFLHKLVVKGFNQKKGIDFDEIFAPVVKMSSIRVVLGLAASLVLEVEQMD-- 447
            ++E  ++  +  +LVVKGFNQKKGIDFDEIF PVVKMSSIRVVLGLAASL LE++QMD  
Sbjct: 764  QEEHTSQPRYKARLVVKGFNQKKGIDFDEIFFPVVKMSSIRVVLGLAASLDLEIQQMDVK 823

Query: 448  ----------------------KGKEHLVCKLKKSLYGLKQAPRKWY------------K 507
                                  KGKE  VCKLKKSLYGLKQAPR+W             K
Sbjct: 824  TAFLHGNLDKEIYMEQPEGFVLKGKEDYVCKLKKSLYGLKQAPRQWNVSRIDNLKKQLSK 883

Query: 508  KF--------ESIMGEQGYRKTTSDHYVFFHKFFDDDFKVLERFKMSQAKSVSSPLPSHF 567
             F        + I+G +  R   S       + + ++  VL RF MS+AK VSSPL SHF
Sbjct: 884  SFAMKDLGPVKRILGIRIERDRASKKLCMLQEQYIEN--VLARFNMSKAKVVSSPLASHF 943

Query: 568  KLISKQSPSTYKEKEDMNK-----------YVMVCTRPDISHVIGVVSRYMYNPGKQHWE 627
            KL S+ SPST KEKEDM +           Y MVCTRPDI++ IGVVSR++ NPG+ HWE
Sbjct: 944  KLSSRHSPSTDKEKEDMRRVPYASAVGSLMYAMVCTRPDIAYAIGVVSRFLSNPGRHHWE 1003

Query: 628  AVKCIMRYLRGTSSLKLTFGNGKPIL-------------------------AGGAVFWQS 687
            AVK IMRYLRGTS LKLTFG+GKPIL                         +GGAV WQS
Sbjct: 1004 AVKWIMRYLRGTSKLKLTFGSGKPILVGYTDSDMAGDVDNRRXTSGYLMTFSGGAVSWQS 1063

Query: 688  RLQKCVALSTTEAEYIAVVETCKEMLWMKRFVQKLGFKQQRYVIYCDNILIRMLSFVQKL 736
            RLQKCVALSTTEA+YIA  E CKE+LW K F+Q+LGFKQQRY +YCDN            
Sbjct: 1064 RLQKCVALSTTEAKYIATAEACKELLWXKCFMQELGFKQQRYXVYCDN------------ 1123


HSP 2 Score: 443.4 bits (1139), Expect = 5.6e-121
Identity = 240/468 (51.28%), Postives = 287/468 (61.32%), Query Frame = 1

Query: 2   IVDADVVNLATQQSSWVIDSGASVHATSKREFFASYTSGDFGSVRMGNDKSANTIGIIDV 61
           + D+DVVN A Q++SWVIDSGAS+HAT +++FF SYTSGDFGSVRMGND SA  IG+ DV
Sbjct: 143 VYDSDVVNFACQETSWVIDSGASIHATPRKDFFTSYTSGDFGSVRMGNDGSAKAIGMGDV 202

Query: 62  HLKNENGSRLILKNVKHIPYIRMNLISTGKLDDKGFCNTFDNGIWKLTKGSMDIAKGQKL 121
            L+  NG+ L LKNVKHIP IRMNLISTGKLDD+GF NTF +  WKLT+GSM IAKG K 
Sbjct: 203 RLETSNGTMLTLKNVKHIPDIRMNLISTGKLDDEGFXNTFRDSQWKLTRGSMXIAKGNKS 262

Query: 122 SSLYYMDAKIIDFDINTMNDEANVKLWHKRLSHISEKGLKILTKKNHLPDPKSTPLKRCP 181
           SSLY M A++ID  IN ++D++  +LWH RL H+SEKGL IL KKN L   K   LKRC 
Sbjct: 263 SSLYLMQARVIDSSINAVDDDSTFELWHNRLGHMSEKGLMILAKKNLLSXMKKGSLKRCA 322

Query: 182 HCLAGKQTRVTFKSSQHSRKPNVLELVYSDVCGPMKTKSLG-----------------VY 241
           HCLAGK TRV FK+  H+RKP +L+LVY DVCGPMKTK+L                  VY
Sbjct: 323 HCLAGKXTRVAFKTLHHTRKPGMLDLVYFDVCGPMKTKTLXGSLYFVTFIDDHSRKIWVY 382

Query: 242 TLKTKDQVLQAFKQFHAFVEKETCENLKCVRTDNGG------------------------ 301
           TLK KDQVL  FKQFHA VE+++ E LKC+RTDNGG                        
Sbjct: 383 TLKXKDQVLDVFKQFHALVERQSGEKLKCIRTDNGGEYSGPFDEYCRQHGIRHQKTPPKT 442

Query: 302 -----------KTLNTVVHVL----------------------NFTPCVPLGSKVPNRIW 361
                      +TL   V  L                      N TPCVPL   VP+RIW
Sbjct: 443 PQLNGLAERMNRTLVERVRCLLSQSQLPRSFWGEALNTVVHVLNLTPCVPLEFDVPDRIW 502

Query: 362 SGKDISYSHLCVFGCKAFVHVPKDDISKFDAKTKACVFLGYGQDEFGYKLYDPVKKKLIR 396
           S  +ISY HL VFGCKAFVH+PKD+ SK DAKT+ CVF+GYGQDE GY+ YD V+KKL+ 
Sbjct: 503 SNNEISYDHLRVFGCKAFVHIPKDERSKLDAKTRPCVFIGYGQDELGYRFYDSVQKKLV- 562

BLAST of Cucsa.235640 vs. TAIR10
Match: ATMG00300.1 (ATMG00300.1 Gag-Pol-related retrotransposon family protein)

HSP 1 Score: 82.4 bits (202), Expect = 1.3e-15
Identity = 45/114 (39.47%), Postives = 66/114 (57.89%), Query Frame = 1

Query: 104 GIWKLTKGSMDIAKGQKLSSLYYMDAKIIDFDIN---TMNDEANVKLWHKRLSHISEKGL 163
           G+ K+ KG   I KG +  SLY +   +   + N   T  DE   +LWH RL+H+S++G+
Sbjct: 27  GVLKVLKGCRTILKGNRHDSLYILQGSVETGESNLAETAKDET--RLWHSRLAHMSQRGM 86

Query: 164 KILTKKNHLPDPKSTPLKRCPHCLAGKQTRVTFKSSQHSRKPNVLELVYSDVCG 215
           ++L KK  L   K + LK C  C+ GK  RV F + QH+ K N L+ V+SD+ G
Sbjct: 87  ELLVKKGFLDSSKVSSLKFCEDCIYGKTHRVNFSTGQHTTK-NPLDYVHSDLWG 137

BLAST of Cucsa.235640 vs. TAIR10
Match: AT4G23160.1 (AT4G23160.1 cysteine-rich RLK (RECEPTOR-like protein kinase) 8)

HSP 1 Score: 72.8 bits (177), Expect = 1.0e-12
Identity = 41/121 (33.88%), Postives = 63/121 (52.07%), Query Frame = 1

Query: 400 KLVVKGFNQKKGIDFDEIFAPVVKMSSIRVVLGLAASLVLEVEQMDKGKEHL-------- 459
           +LV KG+ Q++GIDF E F+PV K++S++++L ++A     + Q+D     L        
Sbjct: 149 RLVAKGYTQQEGIDFIETFSPVCKLTSVKLILAISAIYNFTLHQLDISNAFLNGDLDEEI 208

Query: 460 --------------------VCKLKKSLYGLKQAPRKWYKKFESIMGEQGYRKTTSDHYV 493
                               VC LKKS+YGLKQA R+W+ KF   +   G+ ++ SDH  
Sbjct: 209 YMKLPPGYAARQGDSLPPNAVCYLKKSIYGLKQASRQWFLKFSVTLIGFGFVQSHSDHTY 268


HSP 2 Score: 63.2 bits (152), Expect = 7.9e-10
Identity = 45/161 (27.95%), Postives = 75/161 (46.58%), Query Frame = 1

Query: 548 TRPDISHVIGVVSRYMYNPGKQHWEAVKCIMRYLRGTSSLKLTFG--------------- 607
           TR DIS  +  +S++   P   H +AV  I+ Y++GT    L +                
Sbjct: 391 TRLDISFAVNKLSQFSEAPRLAHQQAVMKILHYIKGTVGQGLFYSSQAEMQLQVFSDASF 450

Query: 608 ----------NGKPILAGGAVF-WQSRLQKCVALSTTEAEYIAVVETCKEMLWMKRFVQK 667
                     NG  +  G ++  W+S+ Q+ V+ S+ EAEY A+     EM+W+ +F ++
Sbjct: 451 QSCKDTRRSTNGYCMFLGTSLISWKSKKQQVVSKSSAEAEYRALSFATDEMMWLAQFFRE 510

Query: 668 LGFKQQR-YVIYCDNILIRMLSFVQKLGKNASFHSRTKHID 682
           L     +  +++CDN      +    +  NA FH RTKHI+
Sbjct: 511 LQLPLSKPTLLFCDN------TAAIHIATNAVFHERTKHIE 545

BLAST of Cucsa.235640 vs. TAIR10
Match: ATMG00810.1 (ATMG00810.1 DNA/RNA polymerases superfamily protein)

HSP 1 Score: 59.3 bits (142), Expect = 1.1e-08
Identity = 42/164 (25.61%), Postives = 71/164 (43.29%), Query Frame = 1

Query: 502 KVLERFKMSQAKSVSSPLPSHFKLISKQSPSTYKEKEDMNKYV-----MVCTRPDISHVI 561
           ++L    M   K +S+PLP   KL S  S + Y +  D    V     +  TRPDIS+ +
Sbjct: 64  QILNNAGMLDCKPMSTPLP--LKLNSSVSTAKYPDPSDFRSIVGALQYLTLTRPDISYAV 123

Query: 562 GVVSRYMYNPGKQHWEAVKCIMRYLRGT--------------------------SSLKLT 621
            +V + M+ P    ++ +K ++RY++GT                          +S + +
Sbjct: 124 NIVCQRMHEPTLADFDLLKRVLRYVKGTIFHGLYIHKNSKLNVQAFCDSDWAGCTSTRRS 183

Query: 622 FGNGKPILAGGAVFWQSRLQKCVALSTTEAEYIAVVETCKEMLW 635
                  L    + W ++ Q  V+ S+TE EY A+  T  E+ W
Sbjct: 184 TTGFCTFLGCNIISWSAKRQPTVSRSSTETEYRALALTAAELTW 225

BLAST of Cucsa.235640 vs. NCBI nr
Match: gi|147784778|emb|CAN75440.1| (hypothetical protein VITISV_007304 [Vitis vinifera])

HSP 1 Score: 503.1 bits (1294), Expect = 8.5e-139
Identity = 264/472 (55.93%), Postives = 310/472 (65.68%), Query Frame = 1

Query: 2   IVDADVVNLATQQSSWVIDSGASVHATSKREFFASYTSGDFGSVRMGNDKSANTIGIIDV 61
           + D+DVVN A Q+S WVID GAS+HAT +++FF SYTSGDFGSVRMGND SA  IG+ DV
Sbjct: 256 VYDSDVVNFACQESXWVIDXGASIHATPQKDFFTSYTSGDFGSVRMGNDGSAKAIGMGDV 315

Query: 62  HLKNENGSRLILKNVKHIPYIRMNLISTGKLDDKGFCNTFDNGIWKLTKGSMDIAKGQKL 121
            L+  NG+ L LKNVKHIP IRMNLISTGKLDD+GFCNTF +  WKLT+GSM IAKG K 
Sbjct: 316 RLETSNGTMLTLKNVKHIPDIRMNLISTGKLDDEGFCNTFRDSQWKLTRGSMVIAKGNKS 375

Query: 122 SSLYYMDAKIIDFDINTMNDEANVKLWHKRLSHISEKGLKILTKKNHLPDPKSTPLKRCP 181
           SSLY M A++ID  IN ++D++  +LWH +L H+SEKGL IL KKN L   K   LKRC 
Sbjct: 376 SSLYLMQARVIDSSINAVDDDSTFELWHNKLGHMSEKGLMILAKKNLLXGMKKGSLKRCA 435

Query: 182 HCLAGKQTRVTFKSSQHSRKPNVLELVYSDVCGPMKTKSLG-----------------VY 241
           HCLAGKQTRV FK+  H+RKP +L+LVYSDV GPMKTK+LG                 VY
Sbjct: 436 HCLAGKQTRVAFKTLHHTRKPGMLDLVYSDVYGPMKTKTLGGSLYFVTFIDDHSRKIWVY 495

Query: 242 TLKTKDQVLQAFKQFHAFVEKETCENLKCVRTDNG------------------------- 301
           TLKTKDQVL  FKQFHA VE+++ E LKC+RTDNG                         
Sbjct: 496 TLKTKDQVLDVFKQFHALVERQSGEKLKCIRTDNGGEYSGPFDEYCRQHGFRHQKTPPKT 555

Query: 302 --------------------------------GKTLNTVVHVLNFTPCVPLGSKVPNRIW 361
                                           G+ LNT+VHVLN TPCVPL   VP+RIW
Sbjct: 556 PQLNGLAERMNRTLVERVRCLLSQSQLPRSFWGEALNTIVHVLNLTPCVPLEFDVPDRIW 615

Query: 362 SGKDISYSHLCVFGCKAFVHVPKDDISKFDAKTKACVFLGYGQDEFGYKLYDPVKKKLIR 396
           S  +ISY HL VFGCKAFVH+PKD+ SK DAKT+ CVF+GYGQDE GYK YD V+KKL R
Sbjct: 616 SNNEISYDHLRVFGCKAFVHIPKDERSKLDAKTRPCVFIGYGQDELGYKFYDLVQKKLXR 675

BLAST of Cucsa.235640 vs. NCBI nr
Match: gi|147784778|emb|CAN75440.1| (hypothetical protein VITISV_007304 [Vitis vinifera])

HSP 1 Score: 311.2 bits (796), Expect = 4.7e-81
Identity = 161/270 (59.63%), Postives = 189/270 (70.00%), Query Frame = 1

Query: 502  KVLERFKMSQAKSVSSPLPSHFKLISKQSPSTYKEKEDMNK-----------YVMVCTRP 561
            KV  RF MS+ K VSSPL SHFKL S+ SPST KEKEDM +           Y MVCTRP
Sbjct: 1039 KVFARFNMSKFKVVSSPLASHFKLSSRHSPSTDKEKEDMRRVPYASAIGSLMYAMVCTRP 1098

Query: 562  DISHVIGVVSRYMYNPGKQHWEAVKCIMRYLRGTSSLKLTFGNGKPIL------------ 621
            DI++ +GVVSR++ NPG+ HWEAVK IMRYLRGTS LKLTFG+GKPIL            
Sbjct: 1099 DIAYAVGVVSRFLSNPGRHHWEAVKWIMRYLRGTSKLKLTFGSGKPILVGYTDSDMAGDV 1158

Query: 622  -------------AGGAVFWQSRLQKCVALSTTEAEYIAVVETCKEMLWMKRFVQKLGFK 681
                         +GGAV WQSRLQKCVALSTTEAEYIA VE CKE+LWMK F+Q+LGFK
Sbjct: 1159 DNRKSTSGYLMTFSGGAVSWQSRLQKCVALSTTEAEYIAAVEACKELLWMKCFMQELGFK 1218

Query: 682  QQRYVIYCDNILIRMLSFVQKLGKNASFHSRTKHIDVRYQWLKDALNDELFELEKIHTNH 736
            QQRYV+YCDN           L KN+++H+R+KHIDVRY W++DALND LFE+EKIHT++
Sbjct: 1219 QQRYVVYCDN------QSAIHLSKNSTYHARSKHIDVRYHWMRDALNDNLFEIEKIHTDN 1278


HSP 2 Score: 109.4 bits (272), Expect = 2.7e-20
Identity = 61/117 (52.14%), Postives = 75/117 (64.10%), Query Frame = 1

Query: 388 EDEVQNRQLFLHKLVVKGFNQKKGIDFDEIFAPVVKMSSIRVVLGLAASLVLEVEQMDKG 447
           ++E  ++  +  +LVVK    K+  D  EI    VK + +   L     +      + KG
Sbjct: 861 QEEHTSQPRYKARLVVKRVQSKESFDL-EIQQMDVKTAFLHGDLDKEIYMEQPEGFVLKG 920

Query: 448 KEHLVCKLKKSLYGLKQAPRKWYKKFESIMGEQGYRKTTSDHYVFFHKFFDDDFKVL 505
           KE  VCKLKKSLYGLKQAPR+WYKKFES+MGEQGYRKTTSDH VF  KF DDDF +L
Sbjct: 921 KEDYVCKLKKSLYGLKQAPRQWYKKFESVMGEQGYRKTTSDHCVFVQKFSDDDFVIL 976


HSP 3 Score: 470.7 bits (1210), Expect = 4.7e-129
Identity = 250/472 (52.97%), Postives = 302/472 (63.98%), Query Frame = 1

Query: 2   IVDADVVNLATQQSSWVIDSGASVHATSKREFFASYTSGDFGSVRMGNDKSANTIGIIDV 61
           + D+DVVN A Q++SWVIDSGAS+HAT +++FF SYT  DFGSVRMGND  A  IG+ DV
Sbjct: 30  VYDSDVVNFACQETSWVIDSGASIHATPRKDFFTSYTFSDFGSVRMGNDGLAKAIGMGDV 89

Query: 62  HLKNENGSRLILKNVKHIPYIRMNLISTGKLDDKGFCNTFDNGIWKLTKGSMDIAKGQKL 121
            L+  NG+ L LKNVKHIP IRMNLIST KLDD+G CNTF +  WKLT+GSM I K  K 
Sbjct: 90  RLETSNGTMLTLKNVKHIPDIRMNLISTRKLDDEGLCNTFRDSQWKLTRGSMVITKENKS 149

Query: 122 SSLYYMDAKIIDFDINTMNDEANVKLWHKRLSHISEKGLKILTKKNHLPDPKSTPLKRCP 181
           SSLY M  ++ID  IN ++D++  KLWH RLSH+SEKGL I+ KKN L   K   LKRC 
Sbjct: 150 SSLYLMQVRVIDSSINAVDDDSTFKLWHNRLSHMSEKGLMIMAKKNLLSGMKKGSLKRCA 209

Query: 182 HCLAGKQTRVTFKSSQHSRKPNVLELVYSDVCGPMKTKSLG-----------------VY 241
           HCL GKQTRV FK+  H+RKP +L+LVYSDVCGPMKTK+LG                 VY
Sbjct: 210 HCLGGKQTRVAFKTLHHTRKPGMLDLVYSDVCGPMKTKTLGGSLYFVTFIDDHSRKIWVY 269

Query: 242 TLKTKDQVLQAFKQFHAFVEKETCENLKCVRTDNGGK----------------------- 301
           TLKTKDQVL  FKQFHA VE+++ E LKC++ DNGG+                       
Sbjct: 270 TLKTKDQVLDVFKQFHALVERQSGEKLKCIQIDNGGEYSSPFDEYCRQHGIRHQKTPPKT 329

Query: 302 -TLNTVVHVLNFT-----PCV----------------------------PLGSKVPNRIW 361
             LN +   +N T      C+                            PL   VP+RIW
Sbjct: 330 PQLNGLAESMNRTLVERVRCLLSQSQLPRSFWGEALNTVVHLLNLTPCVPLEFDVPDRIW 389

Query: 362 SGKDISYSHLCVFGCKAFVHVPKDDISKFDAKTKACVFLGYGQDEFGYKLYDPVKKKLIR 396
           S  +I Y HL VFGCKAFVH+PKD+ISK DAKT+ CVF+GYG DE GY+ YDP++KKL+R
Sbjct: 390 SNNEICYDHLRVFGCKAFVHIPKDEISKLDAKTRPCVFIGYGHDELGYRFYDPMQKKLVR 449

BLAST of Cucsa.235640 vs. NCBI nr
Match: gi|147770163|emb|CAN65406.1| (hypothetical protein VITISV_030853 [Vitis vinifera])

HSP 1 Score: 286.6 bits (732), Expect = 1.3e-73
Identity = 181/391 (46.29%), Postives = 220/391 (56.27%), Query Frame = 1

Query: 388 EDEVQNRQLFLHKLVVKGFNQKKGIDFDEIFAPVVKMSSIRVVLGLAASLVLEVEQMDKG 447
           ++E  ++  +  +LVVKGFNQKK  +               + +      VL      KG
Sbjct: 615 QEEHTSQPRYKARLVVKGFNQKKDKE---------------IYMEQQEGFVL------KG 674

Query: 448 KEHLVCKLKKSLYGLKQAPR----------KWYKKF--------ESIMGEQGYRKTTSDH 507
           KE  V KLKKSLYGLKQAPR          +  K F        + I+G +  R   S  
Sbjct: 675 KEDYVSKLKKSLYGLKQAPRNVSRIDNLKKQLSKSFAMKDLGPVKRILGIRIERDRASKK 734

Query: 508 YVFFHKFFDDDFKVLERFKMSQAKSVSSPLPSHFKLISKQSPSTYKEKEDMNKYVMVCTR 567
                + + +  KVL RF MS+AK VSSPL SHFKL S+ SPST KEKEDM +       
Sbjct: 735 LCMLQEQYIE--KVLARFNMSKAKVVSSPLTSHFKLSSRHSPSTDKEKEDMRRVSYALAG 794

Query: 568 PDISHVIGVVSRYMYNPGKQHWEAVKCIMRYLRGTSSLKLTFGNGKPIL----------- 627
             + +V+               EAVK IMRYLRGTS LKLTFG GKPIL           
Sbjct: 795 GSLMYVM---------------EAVKWIMRYLRGTSKLKLTFGGGKPILVGYTDSDMAGD 854

Query: 628 --------------AGGAVFWQSRLQKCVALSTTEAEYIAVVETCKEMLWMKRFVQKLGF 687
                         +GGAV WQSRLQKCVALSTTEAEYIA  E CKE+LWMK F+Q+LGF
Sbjct: 855 VDNRRSTSGYLMTCSGGAVSWQSRLQKCVALSTTEAEYIAAAEACKELLWMKCFMQELGF 914

Query: 688 KQQRYVIYCDNILIRMLSFVQKLGKNASFHSRTKHIDVRYQWLKDALNDELFELEKIHTN 736
           KQQRYV+YCDN           L KN+++H+R+KHI VRY W++DALND L E+EKIHTN
Sbjct: 915 KQQRYVVYCDN------QSPIHLSKNSTYHARSKHIHVRYHWMRDALNDNLLEIEKIHTN 961


HSP 2 Score: 451.4 bits (1160), Expect = 2.9e-123
Identity = 235/415 (56.63%), Postives = 283/415 (68.19%), Query Frame = 1

Query: 2   IVDADVVNLATQQSSWVIDSGASVHATSKREFFASYTSGDFGSVRMGNDKSANTIGIIDV 61
           + D+DVVN A  ++SWVIDSGAS+HAT +++FF SYTS             A  IG+ DV
Sbjct: 241 VYDSDVVNFACXETSWVIDSGASIHATLRKDFFTSYTS-------------AKAIGMGDV 300

Query: 62  HLKNENGSRLILKNVKHIPYIRMNLISTGKLDDKGFCNTFDNGIWKLTKGSMDIAKGQKL 121
            L+  NG+ L LKNVKHIP IRMNLIST KLDD+GFCNTF +  W LT+GSM IAKG K 
Sbjct: 301 RLETSNGTMLTLKNVKHIPDIRMNLISTRKLDDEGFCNTFRDSQWNLTRGSMVIAKGNKS 360

Query: 122 SSLYYMDAKIIDFDINTMNDEANVKLWHKRLSHISEKGLKILTKKNHLPDPKSTPLKRCP 181
           SSLY M A++ID +IN ++D++  +LWH RL H+SEKGL IL KKN L   K   LKRC 
Sbjct: 361 SSLYLMQARVIDSNINAVDDDSTFELWHNRLGHMSEKGLMILAKKNLLSGMKKGSLKRCA 420

Query: 182 HCLAGKQTRVTFKSSQHSRKPNVLELVYSDVCGPMKTKSLG-----------------VY 241
           HCLAGKQTRV FK+  H+RKP +L+LVYSDVCGPMKTK+L                  VY
Sbjct: 421 HCLAGKQTRVAFKTLHHTRKPGMLDLVYSDVCGPMKTKTLSESLYFVTFIDDHSRKIWVY 480

Query: 242 TLKTKDQVLQAFKQFHAFVEKETCENLKCVRTDNGGKTLNTVVHVLNFTPCVPLGSKVPN 301
           TLKTKDQVL  FKQFHA VE+++ E LKC+R DNGG+             C      VP+
Sbjct: 481 TLKTKDQVLDVFKQFHALVERQSGEKLKCIRIDNGGEYSGPFDEY-----CRQXEFDVPD 540

Query: 302 RIWSGKDISYSHLCVFGCKAFVHVPKDDISKFDAKTKACVFLGYGQDEFGYKLYDPVKKK 361
           RIWS  +ISY HL VFGCKAFVH+PK + SK DAKT+ CVF+GY QDE GY+ YDPV+KK
Sbjct: 541 RIWSNNEISYDHLRVFGCKAFVHIPKYERSKLDAKTRPCVFIGYDQDELGYRFYDPVQKK 600

Query: 362 LIRSRDVVFVEDQTIADIEKI----AKHSDNLIDLSSTSLTQSSTQIEDEVQNRQ 396
           L+RSRDVV +ED TI DI K     ++HS +LIDL    LT   TQ+EDE  + Q
Sbjct: 601 LVRSRDVVXMEDHTIQDIXKTNPMESQHSXDLIDLDPAPLTNLPTQVEDEAHDDQ 637

BLAST of Cucsa.235640 vs. NCBI nr
Match: gi|147776056|emb|CAN69911.1| (hypothetical protein VITISV_027081 [Vitis vinifera])

HSP 1 Score: 304.3 bits (778), Expect = 5.8e-79
Identity = 158/270 (58.52%), Postives = 186/270 (68.89%), Query Frame = 1

Query: 502  KVLERFKMSQAKSVSSPLPSHFKLISKQSPSTYKEKEDMNK-----------YVMVCTRP 561
            K+L RF MS+AK VSSPL SHFKL S+ +PST KEKEDM +           YVMVCTRP
Sbjct: 885  KMLARFNMSKAKVVSSPLASHFKLSSRHNPSTDKEKEDMRRVSYASAVGSLMYVMVCTRP 944

Query: 562  DISHVIGVVSRYMYNPGKQHWEAVKCIMRYLRGTSSLKLTFGNGKPIL------------ 621
            DI++ IGVV R++ NPG+ HWEAVK IMRYLRGTS LKLTFG GKPIL            
Sbjct: 945  DIAYAIGVVIRFLSNPGRHHWEAVKWIMRYLRGTSKLKLTFGGGKPILVGYTDSDMAGDV 1004

Query: 622  -------------AGGAVFWQSRLQKCVALSTTEAEYIAVVETCKEMLWMKRFVQKLGFK 681
                         +GG V WQSR QKCVALSTTEAEYIA  E C E+LWMK F+Q+LGFK
Sbjct: 1005 DNRRSTLGYLMTFSGGTVSWQSRQQKCVALSTTEAEYIAAXEACNELLWMKCFMQELGFK 1064

Query: 682  QQRYVIYCDNILIRMLSFVQKLGKNASFHSRTKHIDVRYQWLKDALNDELFELEKIHTNH 736
            QQRYV+YCDN           L KN+++H+R+KHIDVRY W++DALND LFE+EKIHT++
Sbjct: 1065 QQRYVVYCDN------QSAIHLSKNSTYHARSKHIDVRYHWMRDALNDNLFEIEKIHTDN 1124


HSP 2 Score: 84.7 bits (208), Expect = 7.2e-13
Identity = 44/73 (60.27%), Postives = 55/73 (75.34%), Query Frame = 1

Query: 388 EDEVQNRQLFLHKLVVKGFNQKKGIDFDEIFAPVVKMSSIRVVLGLAASLVLEVEQMDKG 447
           ++E  ++  +  +L+VKGFNQKKG DFDEIF+PVVKMSSIRVVLGLAASL LE++QMD  
Sbjct: 733 QEEHTSQPXYKARLIVKGFNQKKGXDFDEIFSPVVKMSSIRVVLGLAASLDLEIQQMDVK 792

Query: 448 KEHLVCKLKKSLY 461
              L   L K +Y
Sbjct: 793 TAFLHGDLXKEIY 805


HSP 3 Score: 449.1 bits (1154), Expect = 1.5e-122
Identity = 240/442 (54.30%), Postives = 285/442 (64.48%), Query Frame = 1

Query: 32  EFFASYTSGDFGSVRMGNDKSANTIGIIDVHLKNENGSRLILKNVKHIPYIRMNLISTGK 91
           +FF SYTSGDFGSVRMGND SA  IG+ +V L+  NG+ L LKNVKHIP IRMNLISTGK
Sbjct: 189 DFFTSYTSGDFGSVRMGNDDSAKAIGMRNVRLETSNGTMLTLKNVKHIPDIRMNLISTGK 248

Query: 92  LDDKGFCNTFDNGIWKLTKGSMDIAKGQKLSSLYYMDAKIIDFDINTMNDEANVKLWHKR 151
           LDD+GF N F +  WKLT+GSM IAKG K SSLY M  ++ID  IN ++D++  +LWH R
Sbjct: 249 LDDEGFYNIFRDSQWKLTRGSMVIAKGNKSSSLYLMQTRVIDSSINAVDDDSTFELWHNR 308

Query: 152 LSHISEKGLKILTKKNHLPDPKSTPLKRCPHCLAGKQTRVTFKSSQHSRKPNVLELVYSD 211
           L H+SEKGL IL KKN L   K   LKRC HCLAGKQTRV FK+ +++RKP +L+LVYSD
Sbjct: 309 LGHMSEKGLMILAKKNLLSGMKKGSLKRCAHCLAGKQTRVAFKTLRYTRKPGMLDLVYSD 368

Query: 212 VCGPMKTKSLG-----------------VYTLKTKDQVLQAFKQFHAFVEKETCENLKCV 271
           VCGPMKTK+LG                 VYTLKTKDQVL  FKQFHA VE+++ E LKC+
Sbjct: 369 VCGPMKTKTLGGSLYFVTFIDDHSRKIWVYTLKTKDQVLDVFKQFHALVERQSGEKLKCI 428

Query: 272 RTDNGGK------------------------TLNTVVHVLNF------------------ 331
           RTDNGG+                         LN +   +N                   
Sbjct: 429 RTDNGGEYSSPFDEYCRQHGIRHQKTSPKTPQLNGLAERMNRTLVERVRCLLSQSQLPRS 488

Query: 332 ---------------TPCVPLGSKVPNRIWSGKDISYSHLCVFGCKAFVHVPKDDISKFD 391
                          TPCVPL   VP+RIWS  +ISY HL VFGCKAFVH+PKD+ SK D
Sbjct: 489 FWGEALNTVVHVLNLTPCVPLEFDVPDRIWSKNEISYDHLRVFGCKAFVHIPKDERSKLD 548

Query: 392 AKTKACVFLGYGQDEFGYKLYDPVKKKLIRSRDVVFVEDQTIADIEKI----AKHSDNLI 396
           AKT+ CVF+GYGQDE GY+ YDPV+KKL+RSRDVVF+ED TI DIEK     ++HS +LI
Sbjct: 549 AKTRPCVFIGYGQDELGYRFYDPVQKKLVRSRDVVFMEDHTIQDIEKTNPMESQHSGDLI 608

BLAST of Cucsa.235640 vs. NCBI nr
Match: gi|147836424|emb|CAN77602.1| (hypothetical protein VITISV_024474 [Vitis vinifera])

HSP 1 Score: 319.7 bits (818), Expect = 1.3e-83
Identity = 200/428 (46.73%), Postives = 237/428 (55.37%), Query Frame = 1

Query: 388  EDEVQNRQLFLHKLVVKGFNQKKGIDFDEIFAPVVKMSSIRVVLGLAASLVLEVEQMD-- 447
            ++E  ++  +  +LVVKGFNQKKGIDFDEIF PVVKMSSIRVVLGLAASL LE++QMD  
Sbjct: 764  QEEHTSQPRYKARLVVKGFNQKKGIDFDEIFFPVVKMSSIRVVLGLAASLDLEIQQMDVK 823

Query: 448  ----------------------KGKEHLVCKLKKSLYGLKQAPRKWY------------K 507
                                  KGKE  VCKLKKSLYGLKQAPR+W             K
Sbjct: 824  TAFLHGNLDKEIYMEQPEGFVLKGKEDYVCKLKKSLYGLKQAPRQWNVSRIDNLKKQLSK 883

Query: 508  KF--------ESIMGEQGYRKTTSDHYVFFHKFFDDDFKVLERFKMSQAKSVSSPLPSHF 567
             F        + I+G +  R   S       + + ++  VL RF MS+AK VSSPL SHF
Sbjct: 884  SFAMKDLGPVKRILGIRIERDRASKKLCMLQEQYIEN--VLARFNMSKAKVVSSPLASHF 943

Query: 568  KLISKQSPSTYKEKEDMNK-----------YVMVCTRPDISHVIGVVSRYMYNPGKQHWE 627
            KL S+ SPST KEKEDM +           Y MVCTRPDI++ IGVVSR++ NPG+ HWE
Sbjct: 944  KLSSRHSPSTDKEKEDMRRVPYASAVGSLMYAMVCTRPDIAYAIGVVSRFLSNPGRHHWE 1003

Query: 628  AVKCIMRYLRGTSSLKLTFGNGKPIL-------------------------AGGAVFWQS 687
            AVK IMRYLRGTS LKLTFG+GKPIL                         +GGAV WQS
Sbjct: 1004 AVKWIMRYLRGTSKLKLTFGSGKPILVGYTDSDMAGDVDNRRXTSGYLMTFSGGAVSWQS 1063

Query: 688  RLQKCVALSTTEAEYIAVVETCKEMLWMKRFVQKLGFKQQRYVIYCDNILIRMLSFVQKL 736
            RLQKCVALSTTEA+YIA  E CKE+LW K F+Q+LGFKQQRY +YCDN            
Sbjct: 1064 RLQKCVALSTTEAKYIATAEACKELLWXKCFMQELGFKQQRYXVYCDN------------ 1123


HSP 2 Score: 443.4 bits (1139), Expect = 8.0e-121
Identity = 240/468 (51.28%), Postives = 287/468 (61.32%), Query Frame = 1

Query: 2   IVDADVVNLATQQSSWVIDSGASVHATSKREFFASYTSGDFGSVRMGNDKSANTIGIIDV 61
           + D+DVVN A Q++SWVIDSGAS+HAT +++FF SYTSGDFGSVRMGND SA  IG+ DV
Sbjct: 143 VYDSDVVNFACQETSWVIDSGASIHATPRKDFFTSYTSGDFGSVRMGNDGSAKAIGMGDV 202

Query: 62  HLKNENGSRLILKNVKHIPYIRMNLISTGKLDDKGFCNTFDNGIWKLTKGSMDIAKGQKL 121
            L+  NG+ L LKNVKHIP IRMNLISTGKLDD+GF NTF +  WKLT+GSM IAKG K 
Sbjct: 203 RLETSNGTMLTLKNVKHIPDIRMNLISTGKLDDEGFXNTFRDSQWKLTRGSMXIAKGNKS 262

Query: 122 SSLYYMDAKIIDFDINTMNDEANVKLWHKRLSHISEKGLKILTKKNHLPDPKSTPLKRCP 181
           SSLY M A++ID  IN ++D++  +LWH RL H+SEKGL IL KKN L   K   LKRC 
Sbjct: 263 SSLYLMQARVIDSSINAVDDDSTFELWHNRLGHMSEKGLMILAKKNLLSXMKKGSLKRCA 322

Query: 182 HCLAGKQTRVTFKSSQHSRKPNVLELVYSDVCGPMKTKSLG-----------------VY 241
           HCLAGK TRV FK+  H+RKP +L+LVY DVCGPMKTK+L                  VY
Sbjct: 323 HCLAGKXTRVAFKTLHHTRKPGMLDLVYFDVCGPMKTKTLXGSLYFVTFIDDHSRKIWVY 382

Query: 242 TLKTKDQVLQAFKQFHAFVEKETCENLKCVRTDNGG------------------------ 301
           TLK KDQVL  FKQFHA VE+++ E LKC+RTDNGG                        
Sbjct: 383 TLKXKDQVLDVFKQFHALVERQSGEKLKCIRTDNGGEYSGPFDEYCRQHGIRHQKTPPKT 442

Query: 302 -----------KTLNTVVHVL----------------------NFTPCVPLGSKVPNRIW 361
                      +TL   V  L                      N TPCVPL   VP+RIW
Sbjct: 443 PQLNGLAERMNRTLVERVRCLLSQSQLPRSFWGEALNTVVHVLNLTPCVPLEFDVPDRIW 502

Query: 362 SGKDISYSHLCVFGCKAFVHVPKDDISKFDAKTKACVFLGYGQDEFGYKLYDPVKKKLIR 396
           S  +ISY HL VFGCKAFVH+PKD+ SK DAKT+ CVF+GYGQDE GY+ YD V+KKL+ 
Sbjct: 503 SNNEISYDHLRVFGCKAFVHIPKDERSKLDAKTRPCVFIGYGQDELGYRFYDSVQKKLV- 562

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
POLX_TOBAC5.4e-6945.97Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum... [more]
COPIA_DROME3.7e-2528.63Copia protein OS=Drosophila melanogaster GN=GIP PE=1 SV=3[more]
M300_ARATH2.2e-1439.47Uncharacterized mitochondrial protein AtMg00300 OS=Arabidopsis thaliana GN=AtMg0... [more]
M810_ARATH2.0e-0725.61Uncharacterized mitochondrial protein AtMg00810 OS=Arabidopsis thaliana GN=AtMg0... [more]
Match NameE-valueIdentityDescription
A5C9D7_VITVI5.9e-13955.93Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_007304 PE=4 SV=1[more]
A5C9D7_VITVI3.3e-8159.63Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_007304 PE=4 SV=1[more]
A5B9T5_VITVI8.7e-7446.29Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_030853 PE=4 SV=1[more]
A5BAF2_VITVI4.0e-7958.52Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_027081 PE=4 SV=1[more]
A5C540_VITVI9.3e-8446.73Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_024474 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
ATMG00300.11.3e-1539.47ATMG00300.1 Gag-Pol-related retrotransposon family protein[more]
AT4G23160.11.0e-1233.88 cysteine-rich RLK (RECEPTOR-like protein kinase) 8[more]
ATMG00810.11.1e-0825.61ATMG00810.1 DNA/RNA polymerases superfamily protein[more]
Match NameE-valueIdentityDescription
gi|147784778|emb|CAN75440.1|8.5e-13955.93hypothetical protein VITISV_007304 [Vitis vinifera][more]
gi|147784778|emb|CAN75440.1|4.7e-8159.63hypothetical protein VITISV_007304 [Vitis vinifera][more]
gi|147770163|emb|CAN65406.1|1.3e-7346.29hypothetical protein VITISV_030853 [Vitis vinifera][more]
gi|147776056|emb|CAN69911.1|5.8e-7958.52hypothetical protein VITISV_027081 [Vitis vinifera][more]
gi|147836424|emb|CAN77602.1|1.3e-8346.73hypothetical protein VITISV_024474 [Vitis vinifera][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR013103RVT_2
IPR025724GAG-pre-integrase_dom
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0044260 cellular macromolecule metabolic process
biological_process GO:0090304 nucleic acid metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:1901363 heterocyclic compound binding
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0043167 ion binding
molecular_function GO:0097159 organic cyclic compound binding
molecular_function GO:0016740 transferase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.235640.1Cucsa.235640.1mRNA


Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013103Reverse transcriptase, RNA-dependent DNA polymerasePFAMPF07727RVT_2coord: 446..499
score: 1.2E-14coord: 398..445
score: 3.1
IPR025724GAG-pre-integrase domainPFAMPF13976gag_pre-integrscoord: 124..188
score: 3.1
NoneNo IPR availablePANTHERPTHR11439GAG-POL-RELATED RETROTRANSPOSONcoord: 17..649
score: 4.7E
NoneNo IPR availablePANTHERPTHR11439:SF193SUBFAMILY NOT NAMEDcoord: 17..649
score: 4.7E

The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cucsa.235640CSPI01G19390Wild cucumber (PI 183967)cgycpiB348
The following gene(s) are paralogous to this gene:

None