Cucsa.234910 (gene) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.234910
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	Subtilisin-like serine protease
Location	scaffold02004 : 160080 .. 166335 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CCATGTGGATGCAAAAGACTGTATGGTGCATAGTTACACAAATAACTTCAATGCCTTCGCTGCGAAGCTTACTGAGGCGGAGGCCAAAACATTGTCAGGTAAGCATGCTATTTGCTTGATTTGCTTTTTAGACCTTATTATTTAAGAAACAAAAAAAACAAAAAATTCACTTGGTAGTGATTGGGTGTTGTTTATTATCATTTGCTTTTGAACACCCTAATTTTAAGTTAGTTTCTGATACAGAAAGGGGGGATGTGCAGCATGTGATTCCAAATAGATACAGAAAGCTACAAACAACACGATCTTGGGACTTCCTTGGATTTCCTATCAATGCAAAGAGGAAGACAAGACAGGAGAGTGACATTATTGTTGGCCTATTTGATACTGGTAATTCTATTCATTTTTTTTATTTTTTTATTTATTTGTTCTCTAGAAAAATGTTTAATTTTGTATCCATCTTAATGCTTTTAGTTTTAAATATTTCAGGCTAGTTATTTGTATTCACTGTTTTCTGTGGACTACATATCAATTTACAATTTATTTGAAGCTTAGAGTTAATTATGTCACTCTGAAATAGAAACGTTTAATTAGTTAGATTTAGATATAAAAGAAAATTAATCGATTCACTGCATGCAAATGGGGACAAAATTATGTTAAAACAAACTAGTTTAACTAACCTTTAATATATTTTGGTAATTAATTGATTTGTATTTTAAATCTTTACGAGTGTATTAAAAGAGCGATAGGTAAGTTTTACAGTTTGCTATAACTTTGATGGAAAATAGACAACCAATTCTTATATAGTATAGGGTCAGTTTGAAATATAGCAAAATGATTAAATTATTTATAAATATAATAAAATTTTAAAATCTATCAATGATAGACGTTAATAAATGTTGATAGACTTCTATAGATATATATATAATTAATATTGATAAAGACTCATACAAGTCTATTAGTGTAAGTCAACAAGACATGCTATCACAAATATCACATTTTATTTATGATCAACCGATATATATCAAGATAGACAAATATAGTAATCTATTTTCGTCTATCTAGGTCTATCACAAATAGATTGTGATACTTTATTATATTTATATGTATTATTGGGTTTTTTTTAGAACAAAGTGCGAAAAAACCGAGACATGCTAAAAACCAATTTTGCACTCAGTCTAAATGATCTTGTATCCTTGTACCTAGTCTAAAACTATCTTACGATCTTATATCCAAATCTAAGCGATTACTACCCTTGTACCCAACACAAATGATCTTGTACCCAGTCAAAGCGATATTCTCGTATCAAATCTAAATGATTTTGCTACATGATATTGTACTCTTGCAACTCTAAATCATCTTGTACCCTTGTATCCAATCTAAATGATCTGTTAGTGATAGTGATCGATCTCTATTTATCAGGGATAGACAACTGATTGTTTAGATATTACGATTATGTATCAATATTGTTTAAATGTTGCACTAAAGAAAAAAAAGAAGAGGAGTGATAAAGAAGAAGGAAGAAATTTTGGAAGGGAAGTGAAGAAATGACGAACAAAAAGAAGTGGGAATGTGACGAAATTAATAATATGAAGAAAAAAAAGAATATTTGAGGGTTTTGTTACATTTATTTAAACTATTCAATATTATTTAAAGTTTGTTATTTAAAATATTTTCATAATTATAGTGGGAAAATATTGCTATATTCATGTTATTTAATTGAACTAATGATAGGAATTACTCCAACGGCTGATAGCTTTAAAGATGATGGGTATGGTCCTCCACCCAAAAAATGGAAAGGCACATGTGACCATTTTGCCAATTTCAGTGGCTGCAACAAGTAATTTTCGTTTTCGTTTTTGACTAATATCACATATATACACAATATGAATTATGTCTTTCTCCTTTAGTAAATCTTTTTTATATGGGTGAAGTAAACTCATCGGAGCAAGATATTTCAAACTTGATGGCATCACCGAGCCCTTCGACGTTTTGTCGCCCGTAGACGTTAACGGTCATGGCACACACACATCGTCAACAGCGACCGGCAATGTAATCACTGGAGCCAACCTCTCTGGCCTGGCTCAAGGAACTGCCCGTGGCGGTGTGCCATCGGCCAGACTAGCCATGTACAAGGTTTGCTGGATGAGCAACGGATGCTCCGATATGGATCTTCTGGCTGCCTTCGACGCCGCCATACAGGATGGTGTCGATGTCATTTCAATATCGATTGCTGGCATTGGATATGGCAATTACACTGACGACCCGATCTCTATCGGTGCATTTCATGCCATGAAGAAGGGCATCATCACTGTGACGGCCGCCGGGAACAATGGCCCGAGTGCCGGCACTGTTGTGAACCACGCGCCGTGGATCTTGACGGTTGCTGCCAGTTCAATTGATCGGAGATTTATCAGCCCAGTCGAGTTGGGCAACGGGAAGAACATCTCTGTAAGTTTCTTGCATATATTTTTGGATGAATACTTATTAAACTTTTTTTTAAAAAAAACTTATATTTATCTATATTTAGCTACTTTCATTTGTTAAACTTATTGGAATAAATTCAAAACTTAACATACGTTCTTATGCAAATTTTGACAGCATCAAGACAGCATAAAGTTAAGCTGATATTTGTTAAGAAAGAGGATCAAAATATGATTTAATCTAAAGTACTCTTAAAATATATAAAATATTATGTGATAATTCTTTATTTCGTAGTTCTATGTTTGGAAATATTTAAAAAGGATCACCAAATGAGCGTTAATTAATAATTTCCGAACTTTTCTCTAAAGATAATAAGTTGAAAATAAATTACTAAGTAACAAGAAAACAAATATGAATATAAATTAATTAAATTAGACTTAAATGAAAAAATGTTTTTAGTTATTACACTTAAATGTTGACAATAGCCTAGACACTAATTTTTCTATCAAATAATGGCTACTTAACTTATTTACAAAGAACTAAGAAAAGGCAAATTGAGCATATTTTACCTCGAAATATTTCATTTTTTACTTTCCACTAATTGAATTTAGTCTCGTATTTGTATACTTTTTTAACATAAGCTTTCCTATAGTTTTAGTTATGAATTCACTTAGAATGTATAATACTAATAGCTAGAGACAACTGTCCCTAACTGATGAACCCGTAATCTTCTCACTTTCTCTAGCTAATGTAGAATCAATTTTTCAATAATAATTAGGGTTAATATTTAAAATTTATTATATTGTTTAGTAAAAGAGGAATTGCATGTGTTGATGATGGTTGTTGTATTGAATTGTATATGGACAGGGGGTAGGAATAAACTTATTCAATCCAGAGAAAAAAATGTACAAATTAGTGAGTGGAGAAGATGTGGCAAAGAACATAGAGGGCAAAGATAATGCAATGTATTGTGAAGACAAGTCACTTGATCCAATCAAGGTGAAAGACAGTCTTGTGTTTTGCAAATTGATGACATGGGGAGCTGATTCTACTGTCAAATCAGTTGGTGCTGCTGGTGCTATCCTTCAAAGTGATCAGTTTCTTGACAATACTGATATCTTCATGGCCCCTTCCGCTTTGGTTAGTAGCTTTGTTGGAGCTACTATTGACGCCTACATCCACTCCACAAGGTCAAATCTCTTAGTTACATTATGTTTCTACTCAATTTTGTTCTTCATTAAATATCTTAGTTATACCCTTCTCATTATCCAAATAGTTTTTGAGATAAGATTTCATTATTAATCTAAATTAGGGTGTGTATATCAATCGGTCAATGTCAGTTTTGGCTGAAAACAAAAGCTAGCCACTGACACAATAGTTTTCATCATCCAAGCTTGGAATGGATGAGAACAACAATCAAGCAATCGTGTGGGGCAGCCGACCACATACATAAAGGTTCTCATATGTTAGTTCAATCGGTTTTGGACAGTTGATCAGGTTTATCAATTCTCATTTGGTCACCCATAATTCTAAGGGGTTTTTGCAAAAATAGCAAAATTAATATGATAATAGAACCTATGTCACAACATTTTCTAAATTGTCAAAAGTAGCAAATTTAAAAATAATGACTCTCTCATGGTCCTATGATTACCGCGTGATATTACTAATTTTGTCATATTCGCAATTTGCGAAAAGTTAGTGTCATGAGCTGTTTTTTTTTAAATGTTTATGTCATTTAATCCATCTCACTTATTTTAATATAGCATTAGAGTCCTTAAAAAACTCAAACAATTATGAAAGTAGTGCAATTTACTTTATGGCTCCCAATTTCTTAGGGGTATTTTAATCTAGAAAAGTGAAAATTGGGATCAAACTCACAAATGATGAGATTGTAATCCAAAGATACATCATCTTAAAAGGTTATTGAGGATTTTCCAAATTGAAAACATGGAGGGACCTTGTAATCTTTCATTGAAATGGATGGATTACTCTTTTCATTACATAGCCAATTAGTTTTATGATGAGCTTTCACGTAATTCTAGAGTAGAATATGTACGTTCATAGGTAGTAAATATTAACCTTTGGAAAAGTATTGATAAAAAAGTTTGATTATGGGGAATGGGCTGTTTGATTTGATAACAGAACACCAACAGCAGTAATATACAAAACAAGACAACACAGAGCAGCAGCTCCAATTATCGCTCCCTTCTCATCCAGAGGCCCAAATCCAGGCTCCACCCACATTCTAAAGGTATATATAAATACATTTAAAAACTGTTTTTCAGATGTCAAATTGAAATTTCTATGTCATGCATACATACGTCATACATATGCATGCATTAAGATTTAGGGTTTAGGAATATGAGATAGTCGAGGTTGTGAATGTGTTGTAGCCGGACATAGCAGCACCAGGAGTCAACATATTGGCGGGTTACACGCCATTGAAGTCATTGACGGGGCTGAAAGGTGACACTCAATTCTCTAAATTCACACTCATGTCTGGCACTTCCATGGCTTGTCCCCACGTTGCCGCTGCCGCCGCCTATGTCAAATCTTTCCACCCTCTTTGGTCTCCGGCTGCCATTAGATCCGCCTTGCTTACTACCGGTACCTCCCTCTCCCTTCAACATCTTTAATTTTAACATCTTTCAAACATTATAAACATTTATGCCATGCATTAAAGAACACTTGATTTTTGCTTTAAACTCATATAGTAATTAAGACTGTTTTGGTAACCAGTAGACTATATATCCTACAAATCAGAATAGATAACTAAACCAAACCGCACAAACTGAACTATAATGCAAAGCACGTATGTAATAACAAATCATAACATATTTATGAAGATTTGAGTTTTTATCGTTTTTATGTCAAGTTATAATTAACGTCTTAATTAGTTTACGGCTTCGGAACATATATGCAAATGCAATAACCATACTTTTTACAAAATTGATATACAAAAGAAAAAAAAAAAAGCTTAAACAGTAGACTAAAATCTACACTAAAATTTACATGATTCGTCAATGATGTGTTTAGTTATGTGTAAGAGTGAGAGAAGAATTTATTATTAGAGAAAAAAAAGTATCAGAATACACATCAGGTGGCACTAGTACAATTCAAGAGTTTCTAAATTGTACTCCTTTGAAATCCTAGAACAAAAATTGTAAATAAGTTAAATAACATAAATATAAATACGAACCTAAATTGCTTCTTCGACCGGAGCATCATAACATAAACTCAAGGTATTCGAAAACAATACTAAATACTAATGTTTTTATATCTTTATTATGAAGCGAAACCAATTAGTCGACGTGGGAACCCTGACGGAGAATTCGGATATGGTGCCGGCAACCTTAATCCACGTAAAGCCAAAAACCCTGGCCTAATCTACGACCTCAATGAGATGTCATACATTCAATTCCTTTGTAGGGAAGGTTACAGTGGATCTTCAATTGTCATCCTCACTGGAACCAAATCCATCAATTGCGCCACAATAATCCCTGGCCAAGGCTATGACTCTCTCAATTACCCAACCTTTCAACTCAGCCTCCAAAGCTCCCGAGAACCGACGACCGCCGTGTTCTGGCGGGAAGTGACCAATGTCGGCAAGCCTGTTTCAGTTTACAATGCCACGGTCAGGGCTCCCCCTGGAGTAGAGATCACGGTAGAGCCAGCGACTTTGTCGTTCTCCTACCTACATCAAAAGGAGAGGTTTAAGGTTGTTGTGAAGGCCAATCCTTTACCGGCGAACACAATGGTATCGGGTTCGATCACTTGGTTTGATCCTCGATATGTTGTGAGGAGTCCGGTTGTTGTTTATAGTCCGTCGGGATGA

mRNA sequence

CCATGTGGATGCAAAAGACTGTATGGTGCATAGTTACACAAATAACTTCAATGCCTTCGCTGCGAAGCTTACTGAGGCGGAGGCCAAAACATTGTCAGAAAGGGGGGATGTGCAGCATGTGATTCCAAATAGATACAGAAAGCTACAAACAACACGATCTTGGGACTTCCTTGGATTTCCTATCAATGCAAAGAGGAAGACAAGACAGGAGAGTGACATTATTGTTGGCCTATTTGATACTGACGTTAACGGTCATGGCACACACACATCGTCAACAGCGACCGGCAATGTAATCACTGGAGCCAACCTCTCTGGCCTGGCTCAAGGAACTGCCCGTGGCGGTGTGCCATCGGCCAGACTAGCCATGTACAAGGTTTGCTGGATGAGCAACGGATGCTCCGATATGGATCTTCTGGCTGCCTTCGACGCCGCCATACAGGATGGTGTCGATGTCATTTCAATATCGATTGCTGGCATTGGATATGGCAATTACACTGACGACCCGATCTCTATCGGTGCATTTCATGCCATGAAGAAGGGCATCATCACTTCACTTGATCCAATCAAGGTGAAAGACAGTCTTGTGTTTTGCAAATTGATGACATGGGGAGCTGATTCTACTGTCAAATCAGTTGGTGCTGCTGGTGCTATCCTTCAAAGTGATCAGTTTCTTGACAATACTGATATCTTCATGGCCCCTTCCGCTTTGGTTAGTAGCTTTGTTGGAGCTACTATTGACGCCTACATCCACTCCACAAGAACACCAACAGCAGTAATATACAAAACAAGACAACACAGAGCAGCAGCTCCAATTATCGCTCCCTTCTCATCCAGAGGCCCAAATCCAGGCTCCACCCACATTCTAAAGCCGGACATAGCAGCACCAGGAGTCAACATATTGGCGGGTTACACGCCATTGAAGTCATTGACGGGGCTGAAAGGTGACACTCAATTCTCTAAATTCACACTCATGTCTGGCACTTCCATGGCTTGTCCCCACGTTGCCGCTGCCGCCGCCTATGTCAAATCTTTCCACCCTCTTTGGTCTCCGGCTGCCATTAGATCCGCCTTGCTTACTACCGCGAAACCAATTAGTCGACGTGGGAACCCTGACGGAGAATTCGGATATGGTGCCGGCAACCTTAATCCACGTAAAGCCAAAAACCCTGGCCTAATCTACGACCTCAATGAGATGTCATACATTCAATTCCTTTGTAGGGAAGGTTACAGTGGATCTTCAATTGTCATCCTCACTGGAACCAAATCCATCAATTGCGCCACAATAATCCCTGGCCAAGGCTATGACTCTCTCAATTACCCAACCTTTCAACTCAGCCTCCAAAGCTCCCGAGAACCGACGACCGCCGTGTTCTGGCGGGAAGTGACCAATGTCGGCAAGCCTGTTTCAGTTTACAATGCCACGGTCAGGGCTCCCCCTGGAGTAGAGATCACGGTAGAGCCAGCGACTTTGTCGTTCTCCTACCTACATCAAAAGGAGAGGTTTAAGGTTGTTGTGAAGGCCAATCCTTTACCGGCGAACACAATGGTATCGGGTTCGATCACTTGGTTTGATCCTCGATATGTTGTGAGGAGTCCGGTTGTTGTTTATAGTCCGTCGGGATGA

Coding sequence (CDS)

CCATGTGGATGCAAAAGACTGTATGGTGCATAGTTACACAAATAACTTCAATGCCTTCGCTGCGAAGCTTACTGAGGCGGAGGCCAAAACATTGTCAGAAAGGGGGGATGTGCAGCATGTGATTCCAAATAGATACAGAAAGCTACAAACAACACGATCTTGGGACTTCCTTGGATTTCCTATCAATGCAAAGAGGAAGACAAGACAGGAGAGTGACATTATTGTTGGCCTATTTGATACTGACGTTAACGGTCATGGCACACACACATCGTCAACAGCGACCGGCAATGTAATCACTGGAGCCAACCTCTCTGGCCTGGCTCAAGGAACTGCCCGTGGCGGTGTGCCATCGGCCAGACTAGCCATGTACAAGGTTTGCTGGATGAGCAACGGATGCTCCGATATGGATCTTCTGGCTGCCTTCGACGCCGCCATACAGGATGGTGTCGATGTCATTTCAATATCGATTGCTGGCATTGGATATGGCAATTACACTGACGACCCGATCTCTATCGGTGCATTTCATGCCATGAAGAAGGGCATCATCACTTCACTTGATCCAATCAAGGTGAAAGACAGTCTTGTGTTTTGCAAATTGATGACATGGGGAGCTGATTCTACTGTCAAATCAGTTGGTGCTGCTGGTGCTATCCTTCAAAGTGATCAGTTTCTTGACAATACTGATATCTTCATGGCCCCTTCCGCTTTGGTTAGTAGCTTTGTTGGAGCTACTATTGACGCCTACATCCACTCCACAAGAACACCAACAGCAGTAATATACAAAACAAGACAACACAGAGCAGCAGCTCCAATTATCGCTCCCTTCTCATCCAGAGGCCCAAATCCAGGCTCCACCCACATTCTAAAGCCGGACATAGCAGCACCAGGAGTCAACATATTGGCGGGTTACACGCCATTGAAGTCATTGACGGGGCTGAAAGGTGACACTCAATTCTCTAAATTCACACTCATGTCTGGCACTTCCATGGCTTGTCCCCACGTTGCCGCTGCCGCCGCCTATGTCAAATCTTTCCACCCTCTTTGGTCTCCGGCTGCCATTAGATCCGCCTTGCTTACTACCGCGAAACCAATTAGTCGACGTGGGAACCCTGACGGAGAATTCGGATATGGTGCCGGCAACCTTAATCCACGTAAAGCCAAAAACCCTGGCCTAATCTACGACCTCAATGAGATGTCATACATTCAATTCCTTTGTAGGGAAGGTTACAGTGGATCTTCAATTGTCATCCTCACTGGAACCAAATCCATCAATTGCGCCACAATAATCCCTGGCCAAGGCTATGACTCTCTCAATTACCCAACCTTTCAACTCAGCCTCCAAAGCTCCCGAGAACCGACGACCGCCGTGTTCTGGCGGGAAGTGACCAATGTCGGCAAGCCTGTTTCAGTTTACAATGCCACGGTCAGGGCTCCCCCTGGAGTAGAGATCACGGTAGAGCCAGCGACTTTGTCGTTCTCCTACCTACATCAAAAGGAGAGGTTTAAGGTTGTTGTGAAGGCCAATCCTTTACCGGCGAACACAATGGTATCGGGTTCGATCACTTGGTTTGATCCTCGATATGTTGTGAGGAGTCCGGTTGTTGTTTATAGTCCGTCGGGATGA

Protein sequence

HVDAKDCMVHSYTNNFNAFAAKLTEAEAKTLSERGDVQHVIPNRYRKLQTTRSWDFLGFPINAKRKTRQESDIIVGLFDTDVNGHGTHTSSTATGNVITGANLSGLAQGTARGGVPSARLAMYKVCWMSNGCSDMDLLAAFDAAIQDGVDVISISIAGIGYGNYTDDPISIGAFHAMKKGIITSLDPIKVKDSLVFCKLMTWGADSTVKSVGAAGAILQSDQFLDNTDIFMAPSALVSSFVGATIDAYIHSTRTPTAVIYKTRQHRAAAPIIAPFSSRGPNPGSTHILKPDIAAPGVNILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHVAAAAAYVKSFHPLWSPAAIRSALLTTAKPISRRGNPDGEFGYGAGNLNPRKAKNPGLIYDLNEMSYIQFLCREGYSGSSIVILTGTKSINCATIIPGQGYDSLNYPTFQLSLQSSREPTTAVFWREVTNVGKPVSVYNATVRAPPGVEITVEPATLSFSYLHQKERFKVVVKANPLPANTMVSGSITWFDPRYVVRSPVVVYSPSG*

Homology

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BLAST of Cucsa.234910 vs. Swiss-Prot
Match: SBT4E_ARATH (Subtilisin-like protease SBT4.14 OS=Arabidopsis thaliana GN=SBT4.14 PE=2 SV=1)

HSP 1 Score: 451.8 bits (1161), Expect = 1.0e-125
Identity = 224/384 (58.33%), Postives = 287/384 (74.74%), Query Frame = 1


Query: 156 IAGIGYGNYTDDPISIGAFHAMKKGIITSLDPIKVKDSLVFCKLMTWGADSTVKSVGAAG 215
           ++G+     TDD       +  +     SLD  KVK  ++ C++   G +ST+KS G AG
Sbjct: 370 VSGVDAAKNTDDK------YLARYCFSDSLDRKKVKGKVMVCRMGGGGVESTIKSYGGAG 429

Query: 216 AILQSDQFLDNTDIFMAPSALVSSFVGATIDAYIHSTRTPTAVIYKTRQHRAAAPIIAPF 275
           AI+ SDQ+LDN  IFMAP+  V+S VG  I  YI+STR+ +AVI KTRQ    AP +A F
Sbjct: 430 AIIVSDQYLDNAQIFMAPATSVNSSVGDIIYRYINSTRSASAVIQKTRQVTIPAPFVASF 489

Query: 276 SSRGPNPGSTHILKPDIAAPGVNILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHVA 335
           SSRGPNPGS  +LKPDIAAPG++ILA +T  +SLTGL GDTQFSKFT++SGTSMACPHVA
Sbjct: 490 SSRGPNPGSIRLLKPDIAAPGIDILAAFTLKRSLTGLDGDTQFSKFTILSGTSMACPHVA 549

Query: 336 AAAAYVKSFHPLWSPAAIRSALLTTAKPISRRGNPDGEFGYGAGNLNPRKAKNPGLIYDL 395
             AAYVKSFHP W+PAAI+SA++T+AKPISRR N D EF YG G +NPR+A +PGL+YD+
Sbjct: 550 GVAAYVKSFHPDWTPAAIKSAIITSAKPISRRVNKDAEFAYGGGQINPRRAASPGLVYDM 609

Query: 396 NEMSYIQFLCREGYSGSSIVILTGTKSINCATIIPGQGYDSLNYPTFQLSLQSSREPTTA 455
           +++SY+QFLC EGY+ +++  L GT+S++C++I+PG G+DSLNYPT QL+L+S++  T A
Sbjct: 610 DDISYVQFLCGEGYNATTLAPLVGTRSVSCSSIVPGLGHDSLNYPTIQLTLRSAKTSTLA 669

Query: 456 VFWREVTNVGKPVSVYNATVRAPPGVEITVEPATLSFSYLHQKERFKVVVKANPLPANTM 515
           VF R VTNVG P SVY ATVRAP GVEITVEP +LSFS   QK  FKVVVKA  +    +
Sbjct: 670 VFRRRVTNVGPPSSVYTATVRAPKGVEITVEPQSLSFSKASQKRSFKVVVKAKQMTPGKI 729

Query: 516 VSGSITWFDPRYVVRSPVVVYSPS 540
           VSG + W  PR+ VRSP+V+YSP+
Sbjct: 730 VSGLLVWKSPRHSVRSPIVIYSPT 747

HSP 2 Score: 142.9 bits (359), Expect = 1.0e-32
Identity = 66/103 (64.08%), Postives = 84/103 (81.55%), Query Frame = 1


Query: 81  DVNGHGTHTSSTATGNVITGANLSGLAQGTARGGVPSARLAMYKVCWMSNGCSDMDLLAA 140
           D++GHGTHTSST  G ++  A+L G+A GTARG VPSARLAMYKVCW  +GC+DMD+LA 
Sbjct: 206 DIDGHGTHTSSTVAGVLVANASLYGIANGTARGAVPSARLAMYKVCWARSGCADMDILAG 265

Query: 141 FDAAIQDGVDVISISIAGIGYGNYTDDPISIGAFHAMKKGIIT 184
           F+AAI DGV++ISISI G    +Y+ D IS+G+FHAM+KGI+T
Sbjct: 266 FEAAIHDGVEIISISIGG-PIADYSSDSISVGSFHAMRKGILT 307

HSP 3 Score: 89.7 bits (221), Expect = 1.0e-16
Identity = 42/80 (52.50%), Postives = 57/80 (71.25%), Query Frame = 1


Query: 3   DAKDCMVHSYTNNFNAFAAKLTEAEAKTLSERGDVQHVIPNRYRKLQTTRSWDFLGFPIN 62
           +AK+  V+SYT  FNAFAAKL+  EAK + E  +V  V  N+YRKL TT+SWDF+G P+ 
Sbjct: 69  EAKERKVYSYTKAFNAFAAKLSPHEAKKMMEMEEVVSVSRNQYRKLHTTKSWDFVGLPLT 128

Query: 63  AKRKTRQESDIIVGLFDTDV 83
           AKR  + E D+I+G+ DT +
Sbjct: 129 AKRHLKAERDVIIGVLDTGI 148

[Close]

BLAST of Cucsa.234910 vs. Swiss-Prot
Match: SBT4A_ARATH (Subtilisin-like protease SBT4.10 OS=Arabidopsis thaliana GN=SBT4.10 PE=5 SV=1)

HSP 1 Score: 299.7 bits (766), Expect = 6.6e-80
Identity = 192/494 (38.87%), Postives = 258/494 (52.23%), Query Frame = 1


Query: 81  DVNGHGTHTSSTATGNVITGANLSGLAQGTARGGVPSARLAMYKVCWMSNGCSDMDLLAA 140
           D+ GHGTHT+STA GNV+   +  G+  GTARGGVP +R+A YKVC    GCS   +L+A
Sbjct: 196 DIEGHGTHTASTAAGNVVENTSFYGIGNGTARGGVPDSRIAAYKVC-SGAGCSSEYILSA 255

Query: 141 FDAAIQDGVDVISISIAGIGYGNYTDDPISIGAFHAMKKGIITSLD--------PIKVKD 200
           FD AI DGVDVIS S+ G     Y  DPI+IGAFHAM KGI+T            + V  
Sbjct: 256 FDDAIADGVDVISASLGGDTAYMYEKDPIAIGAFHAMAKGILTVQSAGNNGPNPTVSVAP 315

Query: 201 SLVFCKLMTWGADSTVKSVGAAGAIL--QSDQFLD----NTDIFMAPSALVSSFVGATID 260
            ++     T       K V   G  L  QS    D       +    S    +    T  
Sbjct: 316 WILTVAASTTNRRIVTKVVLGNGKTLVGQSVNAFDLKGKQYPLVYETSVEKCNNESLTTL 375

Query: 261 AYIHSTRT------------------PTAVIYKTRQ-HRAAAPIIAPFSSRGPNPGSTHI 320
           A    T T                  P A I K+        P +A FSSRGPN  +  I
Sbjct: 376 ALSFLTLTPQSNEQIISMFHTLIMWSPKATILKSEAIFNQTDPKVAGFSSRGPNTIAVDI 435

Query: 321 LKPDIAAPGVNILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHVAAAAAYVKSFHPL 380
           LKPDI APGV ILA Y+PL S +    D +   +T+ SGTSMACPHV+  AAY+K+FHP 
Sbjct: 436 LKPDITAPGVEILAAYSPLVSPSATTLDNRRVNYTITSGTSMACPHVSGVAAYIKTFHPE 495

Query: 381 WSPAAIRSALLTTAKPISRRGNP--DGEFGYGAGNLNPRKAKNPGLIYDLNEMSYIQFLC 440
           W P+ I+SA++TTA P++  G      EF YG+G+++P  A NPGL+Y+L +  +I FLC
Sbjct: 496 WYPSMIQSAIMTTAWPMNPSGTDAVSTEFAYGSGHIDPIAAINPGLVYELGKSDHIAFLC 555

Query: 441 REGYSGSSIVILTGTKSINCATIIPGQGYDSLNYPTFQLSLQSSREPTTAVFWREVTNVG 500
              Y+ +++ ++ G         +P     +LNYP+    L  S+   T  F R VTNVG
Sbjct: 556 GLNYNATTLKLIAGEAVTCTGKTLP----RNLNYPSMSAKLSKSKSSFTVTFNRTVTNVG 615

Query: 501 KPVSVYNATVRAPPG--VEITVEPATLSFSYLHQKERFKVVVKANPLPANTMVSGSITWF 538
              S Y + V    G  +++ V P+ LS   +++K+ F V V  N L      S ++ W 
Sbjct: 616 TSNSTYKSKVVINHGSKLKVKVSPSVLSMKSVNEKQSFTVSVSGNDLNPKLPSSANLIWS 675

HSP 2 Score: 72.8 bits (177), Expect = 1.3e-11
Identity = 39/90 (43.33%), Postives = 52/90 (57.78%), Query Frame = 1


Query: 8   MVHSYTNNFNAFAAKLTEAEAKTLSERGDVQHVIPNRYRKLQTTRSWDFLGFP--INAKR 67
           +V SY  +FN F+A+LTE+E K ++E   V  V P++  KL TT SWDF+G     N KR
Sbjct: 72  LVRSYKKSFNGFSARLTESERKRVAEMEGVVSVFPSKKYKLHTTASWDFMGLKEGKNTKR 131

Query: 68  KTRQESDIIVGLFDTDVNGHGTHTSSTATG 96
               ESD IVG+FDT ++      S    G
Sbjct: 132 NLAVESDTIVGVFDTGISPESESFSGKGFG 161

[Close]

BLAST of Cucsa.234910 vs. Swiss-Prot
Match: SBT4F_ARATH (Subtilisin-like protease SBT4.15 OS=Arabidopsis thaliana GN=SBT4.15 PE=3 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 1.6e-78
Identity = 167/348 (47.99%), Postives = 226/348 (64.94%), Query Frame = 1


Query: 203 GADSTVKSVGAAGAILQSDQFLDNTDIFMAPSALVS-SFV----GATIDAYIHSTRTPTA 262
           G D  V+S+  AG I+Q    L+ TD  MA S L++ S+V    G  I  YI+ST+ P A
Sbjct: 418 GQDHVVRSLKGAGVIVQ---LLEPTD--MATSTLIAGSYVFFEDGTKITEYINSTKNPQA 477

Query: 263 VIYKTRQHRAAAPIIAPFSSRGPNPGSTHILKPDIAAPGVNILAGYTPLKSLTGLKGDTQ 322
           VI+KT+  +  AP I+ FS+RGP   S +ILKPDI+APG+NILA Y+ L S+TG   D +
Sbjct: 478 VIFKTKTTKMLAPSISSFSARGPQRISPNILKPDISAPGLNILAAYSKLASVTGYPDDNR 537

Query: 323 FSKFTLMSGTSMACPHVAAAAAYVKSFHPLWSPAAIRSALLTTAKPISRRGNPDGEFGYG 382
            + F++MSGTSMACPH AAAAAYVKSFHP WSPAAI+SAL+TTA P+  +GN + E  YG
Sbjct: 538 RTLFSIMSGTSMACPHAAAAAAYVKSFHPDWSPAAIKSALMTTATPMRIKGN-EAELSYG 597

Query: 383 AGNLNPRKAKNPGLIYDLNEMSYIQFLCREGYSGSSIVILTG-------TKSINCATIIP 442
           +G +NPR+A +PGL+YD+ E +Y++FLC+EGY+ +SI +LTG        K  NC  I  
Sbjct: 598 SGQINPRRAIHPGLVYDITEDAYLRFLCKEGYNSTSIGLLTGDNSNNTTKKEYNCENIKR 657

Query: 443 GQGYDSLNYPTFQLSLQSSREPTTAVFWREVTNVGKPVSVYNATVRAPPGVEITVEPATL 502
           G G D LNYP+    + S+    + VF+R VTNVG   S Y A V AP G+ + V P  +
Sbjct: 658 GLGSDGLNYPSLHKQVNSTEAKVSEVFYRTVTNVGYGPSTYVARVWAPKGLRVEVVPKVM 717

Query: 503 SFSYLHQKERFKVVVKAN-PLPANTMVSGSITWFDPR-YVVRSPVVVY 537
           SF    +K  FKVV+          +VS S+ W D R ++VRSP++++
Sbjct: 718 SFERPKEKRNFKVVIDGVWDETMKGIVSASVEWDDSRGHLVRSPILLF 759

HSP 2 Score: 139.0 bits (349), Expect = 1.5e-31
Identity = 75/126 (59.52%), Postives = 92/126 (73.02%), Query Frame = 1


Query: 61  INAKRKTRQESDIIVGLFDT--DVNGHGTHTSSTATGNVITGANLSGLAQGTARGGVPSA 120
           I AK    Q   +  G  DT  D +GHGTHTSST  G  ++ A+L G+A GTARGGVPSA
Sbjct: 184 IGAKYFHIQSEGLPDGEGDTAADHDGHGTHTSSTIAGVSVSSASLFGIANGTARGGVPSA 243

Query: 121 RLAMYKVCWMSNGCSDMDLLAAFDAAIQDGVDVISISIAGIGYGNYTDDPISIGAFHAMK 180
           R+A YKVCW  +GC+DMD+LAAFD AI DGVD+ISISI G     + +DPI+IGAFHAMK
Sbjct: 244 RIAAYKVCW-DSGCTDMDMLAAFDEAISDGVDIISISIGGASL-PFFEDPIAIGAFHAMK 303

Query: 181 KGIITS 185
           +GI+T+
Sbjct: 304 RGILTT 307

HSP 3 Score: 65.5 bits (158), Expect = 2.1e-09
Identity = 36/81 (44.44%), Postives = 47/81 (58.02%), Query Frame = 1


Query: 4   AKDCMVHSYTNNFNAFAAKLTEAEAKTLSERGDVQHVIPNRYRKLQTTRSWDFLGF-PIN 63
           A++  ++SY  N N F A+L   EA+ LS    V  V  N  R+L TTRSWDFLG     
Sbjct: 68  ARELKIYSYGKNINGFVARLFPHEAEKLSREEGVVSVFKNTQRQLHTTRSWDFLGLVESK 127

Query: 64  AKRKTRQESDIIVGLFDTDVN 84
            KR    ES+IIVG+ DT ++
Sbjct: 128 YKRSVGIESNIIVGVLDTGID 148

[Close]

BLAST of Cucsa.234910 vs. Swiss-Prot
Match: CUCM1_CUCME (Cucumisin OS=Cucumis melo PE=1 SV=1)

HSP 1 Score: 252.3 bits (643), Expect = 1.2e-65
Identity = 140/355 (39.44%), Postives = 215/355 (60.56%), Query Frame = 1


Query: 184 SLDPIKVKDSLVFCKLMTWGADSTVKSVGAAGAILQSDQFLDNTDIFMAPSALVSSFVGA 243
           S++P  +K  +V C+  ++G     KS+  A  +L +    D  D +  PS+++      
Sbjct: 384 SVNPNLLKGKIVVCEA-SFGPHEFFKSLDGAAGVLMTSNTRDYADSYPLPSSVLDPNDLL 443

Query: 244 TIDAYIHSTRTPTAVIYK-TRQHRAAAPIIAPFSSRGPNPGSTHILKPDIAAPGVNILAG 303
               YI+S R+P A I+K T    A+AP++  FSSRGPN  +  ++KPDI+ PGV ILA 
Sbjct: 444 ATLRYIYSIRSPGATIFKSTTILNASAPVVVSFSSRGPNRATKDVIKPDISGPGVEILAA 503

Query: 304 YTPLKSLTGLKGDTQFSKFTLMSGTSMACPHVAAAAAYVKSFHPLWSPAAIRSALLTTAK 363
           +  +  + G++ +T F+   ++SGTSM+CPH+   A YVK+++P WSPAAI+SAL+TTA 
Sbjct: 504 WPSVAPVGGIRRNTLFN---IISGTSMSCPHITGIATYVKTYNPTWSPAAIKSALMTTAS 563

Query: 364 PISRRGNPDGEFGYGAGNLNPRKAKNPGLIYDLNEMSYIQFLCREGYSGSSIVILTGTKS 423
           P++ R NP  EF YG+G++NP KA  PGL+YD NE  Y++FLC +GY+  ++  +TG  S
Sbjct: 564 PMNARFNPQAEFAYGSGHVNPLKAVRPGLVYDANESDYVKFLCGQGYNTQAVRRITGDYS 623

Query: 424 INCATIIPGQGYDSLNYPTFQLSLQSSREPTTAVFWREVTNVGKPVSVYNATVRAPPGVE 483
             C +   G+ +D LNYP+F LS+  S +     F R +T+V    S Y A + AP G+ 
Sbjct: 624 A-CTSGNTGRVWD-LNYPSFGLSVSPS-QTFNQYFNRTLTSVAPQASTYRAMISAPQGLT 683

Query: 484 ITVEPATLSFSYLHQKERFKVVVKANPLPANTMVSGSITWFDPRYVVRSPVVVYS 538
           I+V P  LSF+ L  ++ F + V+ +      +VS S+ W D  + VRSP+ + S
Sbjct: 684 ISVNPNVLSFNGLGDRKSFTLTVRGS--IKGFVVSASLVWSDGVHYVRSPITITS 729

HSP 2 Score: 136.0 bits (341), Expect = 1.3e-30
Identity = 64/104 (61.54%), Postives = 83/104 (79.81%), Query Frame = 1


Query: 81  DVNGHGTHTSSTATGNVITGANLSGLAQGTARGGVPSARLAMYKVCWMSNGCSDMDLLAA 140
           D NGHGTHT+STA G +++ ANL GL  GTARGGVP AR+A YKVCW ++GCSD D+LAA
Sbjct: 200 DTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARIAAYKVCW-NDGCSDTDILAA 259

Query: 141 FDAAIQDGVDVISISIAGIGYGNYTDDPISIGAFHAMKKGIITS 185
           +D AI DGVD+IS+S+ G    +Y  D I+IG+FHA+++GI+TS
Sbjct: 260 YDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGILTS 302

HSP 3 Score: 80.1 bits (196), Expect = 8.2e-14
Identity = 36/79 (45.57%), Postives = 53/79 (67.09%), Query Frame = 1


Query: 4   AKDCMVHSYTNNFNAFAAKLTEAEAKTLSERGDVQHVIPNRYRKLQTTRSWDFLGFPINA 63
           A + ++H+Y  +FN FA KLTE EA+ ++    V  V  N   +L TTRSWDFLGFP+  
Sbjct: 65  APESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLGFPLTV 124

Query: 64  KRKTRQESDIIVGLFDTDV 83
            R+++ ES+I+VG+ DT +
Sbjct: 125 PRRSQVESNIVVGVLDTGI 143

[Close]

BLAST of Cucsa.234910 vs. Swiss-Prot
Match: SBT43_ARATH (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana GN=SBT4.3 PE=3 SV=1)

HSP 1 Score: 245.7 bits (626), Expect = 1.1e-63
Identity = 138/357 (38.66%), Postives = 210/357 (58.82%), Query Frame = 1


Query: 185 LDPIKVKDSLVFCKLMTWGADSTVKSVGAAGAILQSDQFLDNTDIFMAPSALVSSFVGAT 244
           +D   VK  +V C       ++ +   GA G I+Q+    D+  +   P++ +      +
Sbjct: 377 VDSELVKGKIVLCDDFLGYREAYL--AGAIGVIVQNTLLPDSAFVVPFPASSLGFEDYKS 436

Query: 245 IDAYIHSTRTPTAVIYKTRQH-RAAAPIIAPFSSRGPNPGSTHILKPDIAAPGVNILAGY 304
           I +YI S   P A I +T +     AP +  FSSRGP+    ++LKPD++APG+ ILA +
Sbjct: 437 IKSYIESAEPPQAEILRTEEIVDREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEILAAF 496

Query: 305 TPLKSLTGLKG--DTQFSKFTLMSGTSMACPHVAAAAAYVKSFHPLWSPAAIRSALLTTA 364
           +P+ S +      D +  ++++MSGTSMACPHVA  AAYVKSFHP WSP+AI+SA++TTA
Sbjct: 497 SPVASPSSFLNPEDKRSVRYSVMSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTA 556

Query: 365 KPISRRGNPDGEFGYGAGNLNPRKAKNPGLIYDLNEMSYIQFLCREGYSGSSIVILTGTK 424
            P++ + NP+ EF YG+G +NP KA +PGL+Y++    Y++ LC EG+  +++   +G +
Sbjct: 557 TPMNLKKNPEQEFAYGSGQINPTKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTTSG-Q 616

Query: 425 SINCATIIPGQGYDSLNYPTFQLSLQSSREPTTAVFWREVTNVGKPVSVYNAT-VRAPPG 484
           ++ C+          LNYPT   +  SS +P    F R VTNVG P S Y A+ V   P 
Sbjct: 617 NVTCS---ERTEVKDLNYPT-MTTFVSSLDPFNVTFKRTVTNVGFPNSTYKASVVPLQPE 676

Query: 485 VEITVEPATLSFSYLHQKERFKVVVKANPLPANTMVSGSITWFDPRYVVRSPVVVYS 538
           ++I++EP  L F +L +K+ F V +    L   + VS S+ W D  + VRSP+V YS
Sbjct: 677 LQISIEPEILRFGFLEEKKSFVVTISGKELKDGSFVSSSVVWSDGSHSVRSPIVAYS 726

HSP 2 Score: 129.8 bits (325), Expect = 9.0e-29
Identity = 65/104 (62.50%), Postives = 80/104 (76.92%), Query Frame = 1


Query: 81  DVNGHGTHTSSTATGNVITGANLSGLAQGTARGGVPSARLAMYKVCWMSNGCSDMDLLAA 140
           D  GHGTHT+STA GN +  A+  GLAQGTARGGVPSAR+A YKVC+  N C+D+D+LAA
Sbjct: 192 DEEGHGTHTASTAAGNAVQAASFYGLAQGTARGGVPSARIAAYKVCF--NRCNDVDILAA 251

Query: 141 FDAAIQDGVDVISISIAGIGYGNYTDDPISIGAFHAMKKGIITS 185
           FD AI DGVDVISISI+     N  +  ++IG+FHAM +GIIT+
Sbjct: 252 FDDAIADGVDVISISISADYVSNLLNASVAIGSFHAMMRGIITA 293

HSP 3 Score: 89.4 bits (220), Expect = 1.3e-16
Identity = 72/232 (31.03%), Postives = 107/232 (46.12%), Query Frame = 1


Query: 2   VDAKDCMVHSYTNNFNAFAAKLTEAEAKTLSERGDVQHVIPNRYRKLQTTRSWDFLGFPI 61
           + A   +V SY  +FN FAA L++AE++ L    +V  V P++  +L TTRSWDF+GF  
Sbjct: 62  IAASHLLVRSYKRSFNGFAANLSQAESQKLQNMKEVVSVFPSKSHELTTTRSWDFVGFGE 121

Query: 62  NAKRKTRQESDIIVGLFDT---------DVNGHGTHTS----------STATGNVITGAN 121
            A+R++ +ESD+IVG+ D+         D  G G                A  N + GA 
Sbjct: 122 KARRESVKESDVIVGVIDSGIWPESESFDDEGFGPPPKKWKGSCKGGLKFACNNKLIGAR 181

Query: 122 LSGLAQGTARG----GVPSARLAMYKVC------WMSNGCSDMDLLAAFDAAIQ------ 181
                  +AR     G  +A  A            ++ G +   + +A  AA +      
Sbjct: 182 FYNKFADSARDEEGHGTHTASTAAGNAVQAASFYGLAQGTARGGVPSARIAAYKVCFNRC 241

Query: 182 --------------DGVDVISISIAGIGYGNYTDDPISIGAFHAMKKGIITS 185
                         DGVDVISISI+     N  +  ++IG+FHAM +GIIT+
Sbjct: 242 NDVDILAAFDDAIADGVDVISISISADYVSNLLNASVAIGSFHAMMRGIITA 293

[Close]

BLAST of Cucsa.234910 vs. TrEMBL
Match: A0A0A0KME6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G000010 PE=4 SV=1)

HSP 1 Score: 552.0 bits (1421), Expect = 8.2e-154
Identity = 271/355 (76.34%), Postives = 306/355 (86.20%), Query Frame = 1


Query: 184 SLDPIKVKDSLVFCKLMTWGADSTVKSVGAAGAILQSDQFLDNTDIFMAPSALVSSFVGA 243
           +LDP KVK SLVFCKL+TWGADS +KS+GA G I+QSD+FLDN DIFMAP+ +VSS VG 
Sbjct: 389 TLDPTKVKGSLVFCKLLTWGADSVIKSIGANGVIIQSDEFLDNADIFMAPATMVSSLVGN 448

Query: 244 TIDAYIHSTRTPTAVIYKTRQHRAAAPIIAPFSSRGPNPGSTHILKPDIAAPGVNILAGY 303
            I  YI STRTPTAVIYKT+Q +A AP++A FSSRGPNPGS  ILKPDIAAPGV+ILA Y
Sbjct: 449 IIYTYIKSTRTPTAVIYKTKQLKAKAPMVASFSSRGPNPGSHRILKPDIAAPGVDILAAY 508

Query: 304 TPLKSLTGLKGDTQFSKFTLMSGTSMACPHVAAAAAYVKSFHPLWSPAAIRSALLTTAKP 363
           TPLKSLTG KGDTQ+SKFTLMSGTSMACPHVAAAAAYVKSFHPLWSPAAIRSALLTTA P
Sbjct: 509 TPLKSLTGQKGDTQYSKFTLMSGTSMACPHVAAAAAYVKSFHPLWSPAAIRSALLTTATP 568

Query: 364 ISRRGNPDGEFGYGAGNLNPRKAKNPGLIYDLNEMSYIQFLCREGYSGSSIVILTGTKSI 423
           ISRR NP+GEF YGAGNLNP +A +PGLIYDLNE+SYIQFLC EGY+GSSI +L+GTKSI
Sbjct: 569 ISRRLNPEGEFAYGAGNLNPSRAISPGLIYDLNEISYIQFLCSEGYTGSSIAVLSGTKSI 628

Query: 424 NCATIIPGQGYDSLNYPTFQLSLQSSREPTTAVFWREVTNVGKPVSVYNATVRAPPGVEI 483
           NC+ +IPGQG+DSLNYPTFQLSL+S+ +P T  F R VTNVG P+SVYNAT+ APPGV I
Sbjct: 629 NCSNLIPGQGHDSLNYPTFQLSLKSTNQPMTTTFRRRVTNVGHPISVYNATINAPPGVTI 688

Query: 484 TVEPATLSFSYLHQKERFKVVVKANPLPANTMVSGSITWFDPRYVVRSPVVVYSP 539
           TV P TLSFS L QK  FKVVVKA+PLP+  MVSGS+ W   ++VVRSP+VVYSP
Sbjct: 689 TVTPPTLSFSRLLQKRSFKVVVKASPLPSAKMVSGSLAWVGAQHVVRSPIVVYSP 743

[Close]

BLAST of Cucsa.234910 vs. TrEMBL
Match: A0A0A0KME6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G000010 PE=4 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 2.2e-42
Identity = 88/107 (82.24%), Postives = 96/107 (89.72%), Query Frame = 1


Query: 77  LFDTDVNGHGTHTSSTATGNVITGANLSGLAQGTARGGVPSARLAMYKVCWMSNGCSDMD 136
           L   D +GHGTHTSSTATGN I GA+LSGLA+GTARGGVPSAR+AMYKVCW S+GCSDMD
Sbjct: 198 LSPVDTDGHGTHTSSTATGNAIAGASLSGLAEGTARGGVPSARVAMYKVCWTSSGCSDMD 257

Query: 137 LLAAFDAAIQDGVDVISISIAGIGYGNYTDDPISIGAFHAMKKGIIT 184
           +LAAFDAAIQDGVDVISISI G G+ NY+DD ISIGAFHAMKKGIIT
Sbjct: 258 ILAAFDAAIQDGVDVISISIGGGGFNNYSDDSISIGAFHAMKKGIIT 304

HSP 2 Score: 120.2 bits (300), Expect = 7.9e-24
Identity = 56/82 (68.29%), Postives = 67/82 (81.71%), Query Frame = 1


Query: 1   HVDAKDCMVHSYTNNFNAFAAKLTEAEAKTLSERGDVQHVIPNRYRKLQTTRSWDFLGFP 60
           H +A + MV+SYT +FNAFAAKL++ EAK LS R DV HVIPN+YRKLQTTRSWDF+G  
Sbjct: 63  HAEASESMVYSYTKSFNAFAAKLSDDEAKLLSTRKDVHHVIPNKYRKLQTTRSWDFIGLS 122

Query: 61  INAKRKTRQESDIIVGLFDTDV 83
            NA+R T+ ESDIIVGLFDT +
Sbjct: 123 SNARRSTKHESDIIVGLFDTGI 144

HSP 3 Score: 480.7 bits (1236), Expect = 2.3e-132
Identity = 227/355 (63.94%), Postives = 285/355 (80.28%), Query Frame = 1


Query: 184 SLDPIKVKDSLVFCKLMTWGADSTVKSVGAAGAILQSDQFLDNTDIFMAPSALVSSFVGA 243
           SLDP KVK  LV+CKL TWGADS +K +G  G I+ S+QFLD   I+MAP  +V+   G 
Sbjct: 392 SLDPKKVKGKLVYCKLGTWGADSVIKGIGGVGIIVGSEQFLDVAQIYMAPGTMVNVTDGD 451

Query: 244 TIDAYIHSTRTPTAVIYKTRQHRAAAPIIAPFSSRGPNPGSTHILKPDIAAPGVNILAGY 303
            I  YIHSTR+P+AVIYK+++ +  AP IA FSSRGPNPGS H+LKPDIAAPG++ILA Y
Sbjct: 452 NITDYIHSTRSPSAVIYKSQEVKVRAPFIASFSSRGPNPGSKHLLKPDIAAPGIDILASY 511

Query: 304 TPLKSLTGLKGDTQFSKFTLMSGTSMACPHVAAAAAYVKSFHPLWSPAAIRSALLTTAKP 363
           T +KSLTGLKGDTQ+SKFTLMSGTSMACPH+A   AYVKSFHP WSPAAI+SA++TTAKP
Sbjct: 512 TLMKSLTGLKGDTQYSKFTLMSGTSMACPHIAGVVAYVKSFHPSWSPAAIKSAIMTTAKP 571

Query: 364 ISRRGNPDGEFGYGAGNLNPRKAKNPGLIYDLNEMSYIQFLCREGYSGSSIVILTGTKSI 423
           +S+R N + EF YGAG +NP+KA +PGL+YD+++MSYIQFLC EGY+GSS+ +L G+KSI
Sbjct: 572 MSQRVNNEAEFAYGAGQVNPQKAVSPGLVYDMDDMSYIQFLCHEGYNGSSLAVLVGSKSI 631

Query: 424 NCATIIPGQGYDSLNYPTFQLSLQSSREPTTAVFWREVTNVGKPVSVYNATVRAPPGVEI 483
           NC ++IPG GYD+LNYPT Q+SL+S+ E TTA+F R VTNVG  +S+YNAT++AP GV I
Sbjct: 632 NCTSLIPGVGYDALNYPTMQVSLKSNGELTTAIFRRRVTNVGPRLSIYNATIKAPKGVNI 691

Query: 484 TVEPATLSFSYLHQKERFKVVVKANPLPANTMVSGSITWFDPRYVVRSPVVVYSP 539
           TV+P +LSFS    K  F VVVKA P+ +  ++SGS+ W  PR+VVRSP+V+Y P
Sbjct: 692 TVKPMSLSFSRTSHKRSFSVVVKAKPMSSTQVLSGSLEWKSPRHVVRSPIVIYRP 746

[Close]

BLAST of Cucsa.234910 vs. TrEMBL
Match: A0A067HDF0_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g004503mg PE=4 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 2.1e-32
Identity = 72/103 (69.90%), Postives = 85/103 (82.52%), Query Frame = 1


Query: 81  DVNGHGTHTSSTATGNVITGANLSGLAQGTARGGVPSARLAMYKVCWMSNGCSDMDLLAA 140
           DV+GHGTHTSST  GNV+  A+L GLA G ARG VP+AR+A YKVCW+S+GCSDMD+LAA
Sbjct: 206 DVDGHGTHTSSTLAGNVVANASLYGLAWGAARGAVPNARVAAYKVCWVSSGCSDMDILAA 265

Query: 141 FDAAIQDGVDVISISIAGIGYGNYTDDPISIGAFHAMKKGIIT 184
           FDAAI DGV+VISISI G    +Y  D IS+GAFHA+KKGI+T
Sbjct: 266 FDAAIHDGVNVISISIGG-ATEDYASDTISVGAFHALKKGIVT 307

HSP 2 Score: 94.7 bits (234), Expect = 3.6e-16
Identity = 45/80 (56.25%), Postives = 57/80 (71.25%), Query Frame = 1


Query: 3   DAKDCMVHSYTNNFNAFAAKLTEAEAKTLSERGDVQHVIPNRYRKLQTTRSWDFLGFPIN 62
           DAK+ +V+SYT +FNAFAAKL+  EA+ L     V  V PNRY +L TTRSWDF+G P  
Sbjct: 69  DAKESIVYSYTESFNAFAAKLSNDEAQKLQRMDRVLSVFPNRYHQLHTTRSWDFIGLPQT 128

Query: 63  AKRKTRQESDIIVGLFDTDV 83
           A+R  + ESDI+VGL DT +
Sbjct: 129 ARRNLKIESDIVVGLMDTGI 148

HSP 3 Score: 474.9 bits (1221), Expect = 1.3e-130
Identity = 229/356 (64.33%), Postives = 278/356 (78.09%), Query Frame = 1


Query: 184 SLDPIKVKDSLVFCKLMTWGADSTVKSVGAAGAILQSDQFLDNTDIFMAPSALVSSFVGA 243
           SLDP KVK  LV CKL  WGADS VK +G  G I++ DQ LD   IFMAP  +V+  +  
Sbjct: 396 SLDPNKVKGKLVLCKLENWGADSVVKGIGGVGTIIEDDQLLDAAQIFMAPGTMVNDTIAE 455

Query: 244 TIDAYIHSTRTPTAVIYKTRQHRAAAPIIAPFSSRGPNPGSTHILKPDIAAPGVNILAGY 303
            ID YIHSTR+P+AVI K+++ +  AP IA FSSRGPNPGS  ILKPDIAAPGV+ILA Y
Sbjct: 456 IIDEYIHSTRSPSAVISKSQEAKKRAPFIASFSSRGPNPGSERILKPDIAAPGVDILASY 515

Query: 304 TPLKSLTGLKGDTQFSKFTLMSGTSMACPHVAAAAAYVKSFHPLWSPAAIRSALLTTAKP 363
           T  KSLTGL+GDTQ+S+FTLMSGTSM+CPHVA   AYVKSFHP WSPAA+RSA+LTTAKP
Sbjct: 516 TLRKSLTGLEGDTQYSEFTLMSGTSMSCPHVAGVVAYVKSFHPHWSPAALRSAILTTAKP 575

Query: 364 ISRRGNPDGEFGYGAGNLNPRKAKNPGLIYDLN-EMSYIQFLCREGYSGSSIVILTGTKS 423
           +S+RGN D E  YG G +NP KA NPGL+YD++  +SYIQFLC EGY GSS+  L G+KS
Sbjct: 576 MSQRGNSDAELSYGVGQVNPTKAVNPGLVYDMDGTLSYIQFLCHEGYKGSSLAPLVGSKS 635

Query: 424 INCATIIPGQGYDSLNYPTFQLSLQSSREPTTAVFWREVTNVGKPVSVYNATVRAPPGVE 483
           +NC++++PGQGYD+LNYPT QLSL+SS++PT  VF R VTNVG   S YNAT+RAP GVE
Sbjct: 636 VNCSSLLPGQGYDALNYPTMQLSLKSSKQPTVGVFRRTVTNVGPAKSTYNATIRAPKGVE 695

Query: 484 ITVEPATLSFSYLHQKERFKVVVKANPLPANTMVSGSITWFDPRYVVRSPVVVYSP 539
           I V+P +LSFS   +K  F+VVVKANP+P+  M+SGS+ W   RY VRSP+V++SP
Sbjct: 696 IIVKPMSLSFSTTLEKRTFQVVVKANPMPSMRMISGSLGWKSSRYTVRSPIVIHSP 751

[Close]

BLAST of Cucsa.234910 vs. TrEMBL
Match: M5X7G0_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa021240mg PE=4 SV=1)

HSP 1 Score: 154.8 bits (390), Expect = 2.9e-34
Identity = 75/103 (72.82%), Postives = 86/103 (83.50%), Query Frame = 1


Query: 81  DVNGHGTHTSSTATGNVITGANLSGLAQGTARGGVPSARLAMYKVCWMSNGCSDMDLLAA 140
           DV+GHGTHTSST  GN +  A+L GLA GTARG VPSAR+AMYKVCW S+GC+DMD+LAA
Sbjct: 210 DVDGHGTHTSSTLAGNQVPNASLFGLAMGTARGAVPSARVAMYKVCWASSGCTDMDILAA 269

Query: 141 FDAAIQDGVDVISISIAGIGYGNYTDDPISIGAFHAMKKGIIT 184
           +DAAI DGVDVIS SI+G G  NY  D ++IGAFHAMKKGIIT
Sbjct: 270 YDAAIHDGVDVISFSISG-GVANYVRDSVAIGAFHAMKKGIIT 311

HSP 2 Score: 102.1 bits (253), Expect = 2.2e-18
Identity = 47/82 (57.32%), Postives = 63/82 (76.83%), Query Frame = 1


Query: 1   HVDAKDCMVHSYTNNFNAFAAKLTEAEAKTLSERGDVQHVIPNRYRKLQTTRSWDFLGFP 60
           +V+A++ MV+SYT +FNAFAAKL+  EA+ LS   +V  V+PNRY KL TT+SWDF+G P
Sbjct: 71  YVEAEESMVYSYTKSFNAFAAKLSSDEAERLSGMDEVLSVLPNRYHKLHTTKSWDFIGLP 130

Query: 61  INAKRKTRQESDIIVGLFDTDV 83
           + A+R  + E DIIVGLFDT +
Sbjct: 131 LKARRNLKLERDIIVGLFDTGI 152

HSP 3 Score: 473.4 bits (1217), Expect = 3.7e-130
Identity = 220/355 (61.97%), Postives = 285/355 (80.28%), Query Frame = 1


Query: 184 SLDPIKVKDSLVFCKLMTWGADSTVKSVGAAGAILQSDQFLDNTDIFMAPSALVSSFVGA 243
           SLDP KVK  LV C+L  WGADS VK +G  G IL+S+Q+LD   IFMAP+ +V++ V  
Sbjct: 353 SLDPKKVKGKLVLCELEVWGADSVVKGIGGKGTILESEQYLDAAQIFMAPATVVNATVSD 412

Query: 244 TIDAYIHSTRTPTAVIYKTRQHRAAAPIIAPFSSRGPNPGSTHILKPDIAAPGVNILAGY 303
            ++ YIHST++P+AVIY+T++ +  AP IA FSSRGPNPGS  ILKPD+AAPG++ILA Y
Sbjct: 413 KVNNYIHSTKSPSAVIYRTQEVKVPAPFIASFSSRGPNPGSERILKPDVAAPGIDILASY 472

Query: 304 TPLKSLTGLKGDTQFSKFTLMSGTSMACPHVAAAAAYVKSFHPLWSPAAIRSALLTTAKP 363
           TPL+SLTGLKGDTQ S+F+LMSGTSMACPHVA  AAY+KSFHP W+ AAI+SA+LTTAKP
Sbjct: 473 TPLRSLTGLKGDTQHSRFSLMSGTSMACPHVAGVAAYIKSFHPNWTAAAIKSAILTTAKP 532

Query: 364 ISRRGNPDGEFGYGAGNLNPRKAKNPGLIYDLNEMSYIQFLCREGYSGSSIVILTGTKSI 423
           +S R N D EF YGAG +NP KA+NPGL+YD++EMSYIQFLC EGY+ SS+ +L G+KS+
Sbjct: 533 MSSRVNNDAEFAYGAGQVNPDKARNPGLVYDMDEMSYIQFLCHEGYNRSSLAVLVGSKSV 592

Query: 424 NCATIIPGQGYDSLNYPTFQLSLQSSREPTTAVFWREVTNVGKPVSVYNATVRAPPGVEI 483
           NC++++PG GYD+LNYPT QLS+++  EPT  VF R VTNVG   S+YNAT++AP GV+I
Sbjct: 593 NCSSLLPGIGYDALNYPTMQLSVKNKHEPTVGVFIRTVTNVGPSPSIYNATIQAPKGVDI 652

Query: 484 TVEPATLSFSYLHQKERFKVVVKANPLPANTMVSGSITWFDPRYVVRSPVVVYSP 539
            V+P +LSFS   QK  FKVVVKA P+P++ M+SGS+ W   +++V+SP+V++ P
Sbjct: 653 VVKPMSLSFSRSSQKRSFKVVVKAKPMPSSQMLSGSLVWKSNQHIVKSPIVIFKP 707

[Close]

BLAST of Cucsa.234910 vs. TrEMBL
Match: B9GPT5_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0002s15330g PE=4 SV=2)

HSP 1 Score: 150.2 bits (378), Expect = 7.2e-33
Identity = 73/107 (68.22%), Postives = 87/107 (81.31%), Query Frame = 1


Query: 77  LFDTDVNGHGTHTSSTATGNVITGANLSGLAQGTARGGVPSARLAMYKVCWMSNGCSDMD 136
           L   DV+GHGTHTSST  GN++  A+L GLA+G ARG VP AR+AMYKVCW+S+GCSDMD
Sbjct: 164 LSPVDVDGHGTHTSSTLAGNLVPDASLFGLARGVARGAVPDARVAMYKVCWVSSGCSDMD 223

Query: 137 LLAAFDAAIQDGVDVISISIAGIGYGNYTDDPISIGAFHAMKKGIIT 184
           LLAAF+AAI DGVDV+SISI G+   +Y  + I+IGAFHAMK GIIT
Sbjct: 224 LLAAFEAAIHDGVDVLSISIGGVS-ADYVSNAIAIGAFHAMKNGIIT 269

HSP 2 Score: 93.2 bits (230), Expect = 1.0e-15
Identity = 43/80 (53.75%), Postives = 58/80 (72.50%), Query Frame = 1


Query: 3   DAKDCMVHSYTNNFNAFAAKLTEAEAKTLSERGDVQHVIPNRYRKLQTTRSWDFLGFPIN 62
           + K+ +++SYT  FNAFAAKL++AEA+ LS   +V  V PNRY KL TT+SWDF+G P  
Sbjct: 31  EQKESIIYSYTKIFNAFAAKLSKAEARKLSLLDEVLSVFPNRYHKLHTTKSWDFIGLPST 90

Query: 63  AKRKTRQESDIIVGLFDTDV 83
           AKR  + E +I+VGL DT +
Sbjct: 91  AKRNLKMERNIVVGLLDTGI 110

HSP 3 Score: 472.6 bits (1215), Expect = 6.3e-130
Identity = 224/355 (63.10%), Postives = 281/355 (79.15%), Query Frame = 1


Query: 184 SLDPIKVKDSLVFCKLMTWGADSTVKSVGAAGAILQSDQFLDNTDIFMAPSALVSSFVGA 243
           S+D  KVK  LV+C+L  WG+DS VK +G  GAI++S Q+LD   IFM P  +V+  VG 
Sbjct: 389 SMDSNKVKGKLVYCELQMWGSDSVVKGIGGVGAIIESAQYLDAAQIFMTPGTMVNVTVGD 448

Query: 244 TIDAYIHSTRTPTAVIYKTRQHRAAAPIIAPFSSRGPNPGSTHILKPDIAAPGVNILAGY 303
           TI+ YIHST++P+AVIY++ + +  AP IA FSSRGPNPGS  +LKPDIAAPG++ILA Y
Sbjct: 449 TINDYIHSTKSPSAVIYRSHEVKIPAPFIASFSSRGPNPGSKLLLKPDIAAPGIDILASY 508

Query: 304 TPLKSLTGLKGDTQFSKFTLMSGTSMACPHVAAAAAYVKSFHPLWSPAAIRSALLTTAKP 363
           TPL SLTGLKGDTQ+SKFTLMSGTSMACPHVA  AAY+KSFHP WS AAI+SA+LTTAKP
Sbjct: 509 TPLHSLTGLKGDTQYSKFTLMSGTSMACPHVAGVAAYIKSFHPNWSAAAIKSAILTTAKP 568

Query: 364 ISRRGNPDGEFGYGAGNLNPRKAKNPGLIYDLNEMSYIQFLCREGYSGSSIVILTGTKSI 423
           +S R N + EF YGAG LNP +A++PGL+YD++EMSYIQFLC EGY+GSS+ +L G+KSI
Sbjct: 569 MSARVNSEAEFAYGAGQLNPSRARSPGLVYDMDEMSYIQFLCHEGYTGSSLAVLIGSKSI 628

Query: 424 NCATIIPGQGYDSLNYPTFQLSLQSSREPTTAVFWREVTNVGKPVSVYNATVRAPPGVEI 483
           NC++++PG GYD++NYPT  LS ++ ++PT  VF R VTNVG   S YNAT++AP GVEI
Sbjct: 629 NCSSLLPGLGYDAINYPTMHLSARNDKQPTIGVFRRTVTNVGPSTSFYNATIKAPKGVEI 688

Query: 484 TVEPATLSFSYLHQKERFKVVVKANPLPANTMVSGSITWFDPRYVVRSPVVVYSP 539
           TV PA+LSFS   QK  FKVVVKA P+ +  ++SGS+ W   R+VVRSP+VVY P
Sbjct: 689 TVVPASLSFSRTLQKRSFKVVVKAKPMSSGQILSGSVAWKSSRHVVRSPIVVYKP 743

[Close]

BLAST of Cucsa.234910 vs. TAIR10
Match: AT4G00230.1 (AT4G00230.1 xylem serine peptidase 1)

HSP 1 Score: 451.8 bits (1161), Expect = 5.8e-127
Identity = 224/384 (58.33%), Postives = 287/384 (74.74%), Query Frame = 1


Query: 156 IAGIGYGNYTDDPISIGAFHAMKKGIITSLDPIKVKDSLVFCKLMTWGADSTVKSVGAAG 215
           ++G+     TDD       +  +     SLD  KVK  ++ C++   G +ST+KS G AG
Sbjct: 370 VSGVDAAKNTDDK------YLARYCFSDSLDRKKVKGKVMVCRMGGGGVESTIKSYGGAG 429

Query: 216 AILQSDQFLDNTDIFMAPSALVSSFVGATIDAYIHSTRTPTAVIYKTRQHRAAAPIIAPF 275
           AI+ SDQ+LDN  IFMAP+  V+S VG  I  YI+STR+ +AVI KTRQ    AP +A F
Sbjct: 430 AIIVSDQYLDNAQIFMAPATSVNSSVGDIIYRYINSTRSASAVIQKTRQVTIPAPFVASF 489

Query: 276 SSRGPNPGSTHILKPDIAAPGVNILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHVA 335
           SSRGPNPGS  +LKPDIAAPG++ILA +T  +SLTGL GDTQFSKFT++SGTSMACPHVA
Sbjct: 490 SSRGPNPGSIRLLKPDIAAPGIDILAAFTLKRSLTGLDGDTQFSKFTILSGTSMACPHVA 549

Query: 336 AAAAYVKSFHPLWSPAAIRSALLTTAKPISRRGNPDGEFGYGAGNLNPRKAKNPGLIYDL 395
             AAYVKSFHP W+PAAI+SA++T+AKPISRR N D EF YG G +NPR+A +PGL+YD+
Sbjct: 550 GVAAYVKSFHPDWTPAAIKSAIITSAKPISRRVNKDAEFAYGGGQINPRRAASPGLVYDM 609

Query: 396 NEMSYIQFLCREGYSGSSIVILTGTKSINCATIIPGQGYDSLNYPTFQLSLQSSREPTTA 455
           +++SY+QFLC EGY+ +++  L GT+S++C++I+PG G+DSLNYPT QL+L+S++  T A
Sbjct: 610 DDISYVQFLCGEGYNATTLAPLVGTRSVSCSSIVPGLGHDSLNYPTIQLTLRSAKTSTLA 669

Query: 456 VFWREVTNVGKPVSVYNATVRAPPGVEITVEPATLSFSYLHQKERFKVVVKANPLPANTM 515
           VF R VTNVG P SVY ATVRAP GVEITVEP +LSFS   QK  FKVVVKA  +    +
Sbjct: 670 VFRRRVTNVGPPSSVYTATVRAPKGVEITVEPQSLSFSKASQKRSFKVVVKAKQMTPGKI 729

Query: 516 VSGSITWFDPRYVVRSPVVVYSPS 540
           VSG + W  PR+ VRSP+V+YSP+
Sbjct: 730 VSGLLVWKSPRHSVRSPIVIYSPT 747

HSP 2 Score: 142.9 bits (359), Expect = 5.8e-34
Identity = 66/103 (64.08%), Postives = 84/103 (81.55%), Query Frame = 1


Query: 81  DVNGHGTHTSSTATGNVITGANLSGLAQGTARGGVPSARLAMYKVCWMSNGCSDMDLLAA 140
           D++GHGTHTSST  G ++  A+L G+A GTARG VPSARLAMYKVCW  +GC+DMD+LA 
Sbjct: 206 DIDGHGTHTSSTVAGVLVANASLYGIANGTARGAVPSARLAMYKVCWARSGCADMDILAG 265

Query: 141 FDAAIQDGVDVISISIAGIGYGNYTDDPISIGAFHAMKKGIIT 184
           F+AAI DGV++ISISI G    +Y+ D IS+G+FHAM+KGI+T
Sbjct: 266 FEAAIHDGVEIISISIGG-PIADYSSDSISVGSFHAMRKGILT 307

HSP 3 Score: 89.7 bits (221), Expect = 5.8e-18
Identity = 42/80 (52.50%), Postives = 57/80 (71.25%), Query Frame = 1


Query: 3   DAKDCMVHSYTNNFNAFAAKLTEAEAKTLSERGDVQHVIPNRYRKLQTTRSWDFLGFPIN 62
           +AK+  V+SYT  FNAFAAKL+  EAK + E  +V  V  N+YRKL TT+SWDF+G P+ 
Sbjct: 69  EAKERKVYSYTKAFNAFAAKLSPHEAKKMMEMEEVVSVSRNQYRKLHTTKSWDFVGLPLT 128

Query: 63  AKRKTRQESDIIVGLFDTDV 83
           AKR  + E D+I+G+ DT +
Sbjct: 129 AKRHLKAERDVIIGVLDTGI 148

[Close]

BLAST of Cucsa.234910 vs. TAIR10
Match: AT5G03620.1 (AT5G03620.1 Subtilisin-like serine endopeptidase family protein)

HSP 1 Score: 295.0 bits (754), Expect = 9.1e-80
Identity = 167/348 (47.99%), Postives = 226/348 (64.94%), Query Frame = 1


Query: 203 GADSTVKSVGAAGAILQSDQFLDNTDIFMAPSALVS-SFV----GATIDAYIHSTRTPTA 262
           G D  V+S+  AG I+Q    L+ TD  MA S L++ S+V    G  I  YI+ST+ P A
Sbjct: 418 GQDHVVRSLKGAGVIVQ---LLEPTD--MATSTLIAGSYVFFEDGTKITEYINSTKNPQA 477

Query: 263 VIYKTRQHRAAAPIIAPFSSRGPNPGSTHILKPDIAAPGVNILAGYTPLKSLTGLKGDTQ 322
           VI+KT+  +  AP I+ FS+RGP   S +ILKPDI+APG+NILA Y+ L S+TG   D +
Sbjct: 478 VIFKTKTTKMLAPSISSFSARGPQRISPNILKPDISAPGLNILAAYSKLASVTGYPDDNR 537

Query: 323 FSKFTLMSGTSMACPHVAAAAAYVKSFHPLWSPAAIRSALLTTAKPISRRGNPDGEFGYG 382
            + F++MSGTSMACPH AAAAAYVKSFHP WSPAAI+SAL+TTA P+  +GN + E  YG
Sbjct: 538 RTLFSIMSGTSMACPHAAAAAAYVKSFHPDWSPAAIKSALMTTATPMRIKGN-EAELSYG 597

Query: 383 AGNLNPRKAKNPGLIYDLNEMSYIQFLCREGYSGSSIVILTG-------TKSINCATIIP 442
           +G +NPR+A +PGL+YD+ E +Y++FLC+EGY+ +SI +LTG        K  NC  I  
Sbjct: 598 SGQINPRRAIHPGLVYDITEDAYLRFLCKEGYNSTSIGLLTGDNSNNTTKKEYNCENIKR 657

Query: 443 GQGYDSLNYPTFQLSLQSSREPTTAVFWREVTNVGKPVSVYNATVRAPPGVEITVEPATL 502
           G G D LNYP+    + S+    + VF+R VTNVG   S Y A V AP G+ + V P  +
Sbjct: 658 GLGSDGLNYPSLHKQVNSTEAKVSEVFYRTVTNVGYGPSTYVARVWAPKGLRVEVVPKVM 717

Query: 503 SFSYLHQKERFKVVVKAN-PLPANTMVSGSITWFDPR-YVVRSPVVVY 537
           SF    +K  FKVV+          +VS S+ W D R ++VRSP++++
Sbjct: 718 SFERPKEKRNFKVVIDGVWDETMKGIVSASVEWDDSRGHLVRSPILLF 759

HSP 2 Score: 139.0 bits (349), Expect = 8.4e-33
Identity = 75/126 (59.52%), Postives = 92/126 (73.02%), Query Frame = 1


Query: 61  INAKRKTRQESDIIVGLFDT--DVNGHGTHTSSTATGNVITGANLSGLAQGTARGGVPSA 120
           I AK    Q   +  G  DT  D +GHGTHTSST  G  ++ A+L G+A GTARGGVPSA
Sbjct: 184 IGAKYFHIQSEGLPDGEGDTAADHDGHGTHTSSTIAGVSVSSASLFGIANGTARGGVPSA 243

Query: 121 RLAMYKVCWMSNGCSDMDLLAAFDAAIQDGVDVISISIAGIGYGNYTDDPISIGAFHAMK 180
           R+A YKVCW  +GC+DMD+LAAFD AI DGVD+ISISI G     + +DPI+IGAFHAMK
Sbjct: 244 RIAAYKVCW-DSGCTDMDMLAAFDEAISDGVDIISISIGGASL-PFFEDPIAIGAFHAMK 303

Query: 181 KGIITS 185
           +GI+T+
Sbjct: 304 RGILTT 307

HSP 3 Score: 65.5 bits (158), Expect = 1.2e-10
Identity = 36/81 (44.44%), Postives = 47/81 (58.02%), Query Frame = 1


Query: 4   AKDCMVHSYTNNFNAFAAKLTEAEAKTLSERGDVQHVIPNRYRKLQTTRSWDFLGF-PIN 63
           A++  ++SY  N N F A+L   EA+ LS    V  V  N  R+L TTRSWDFLG     
Sbjct: 68  ARELKIYSYGKNINGFVARLFPHEAEKLSREEGVVSVFKNTQRQLHTTRSWDFLGLVESK 127

Query: 64  AKRKTRQESDIIVGLFDTDVN 84
            KR    ES+IIVG+ DT ++
Sbjct: 128 YKRSVGIESNIIVGVLDTGID 148

[Close]

BLAST of Cucsa.234910 vs. TAIR10
Match: AT5G59190.1 (AT5G59190.1 subtilase family protein)

HSP 1 Score: 245.7 bits (626), Expect = 6.3e-65
Identity = 138/357 (38.66%), Postives = 210/357 (58.82%), Query Frame = 1


Query: 185 LDPIKVKDSLVFCKLMTWGADSTVKSVGAAGAILQSDQFLDNTDIFMAPSALVSSFVGAT 244
           +D   VK  +V C       ++ +   GA G I+Q+    D+  +   P++ +      +
Sbjct: 341 VDSELVKGKIVLCDDFLGYREAYL--AGAIGVIVQNTLLPDSAFVVPFPASSLGFEDYKS 400

Query: 245 IDAYIHSTRTPTAVIYKTRQH-RAAAPIIAPFSSRGPNPGSTHILKPDIAAPGVNILAGY 304
           I +YI S   P A I +T +     AP +  FSSRGP+    ++LKPD++APG+ ILA +
Sbjct: 401 IKSYIESAEPPQAEILRTEEIVDREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEILAAF 460

Query: 305 TPLKSLTGLKG--DTQFSKFTLMSGTSMACPHVAAAAAYVKSFHPLWSPAAIRSALLTTA 364
           +P+ S +      D +  ++++MSGTSMACPHVA  AAYVKSFHP WSP+AI+SA++TTA
Sbjct: 461 SPVASPSSFLNPEDKRSVRYSVMSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTA 520

Query: 365 KPISRRGNPDGEFGYGAGNLNPRKAKNPGLIYDLNEMSYIQFLCREGYSGSSIVILTGTK 424
            P++ + NP+ EF YG+G +NP KA +PGL+Y++    Y++ LC EG+  +++   +G +
Sbjct: 521 TPMNLKKNPEQEFAYGSGQINPTKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTTSG-Q 580

Query: 425 SINCATIIPGQGYDSLNYPTFQLSLQSSREPTTAVFWREVTNVGKPVSVYNAT-VRAPPG 484
           ++ C+          LNYPT   +  SS +P    F R VTNVG P S Y A+ V   P 
Sbjct: 581 NVTCS---ERTEVKDLNYPT-MTTFVSSLDPFNVTFKRTVTNVGFPNSTYKASVVPLQPE 640

Query: 485 VEITVEPATLSFSYLHQKERFKVVVKANPLPANTMVSGSITWFDPRYVVRSPVVVYS 538
           ++I++EP  L F +L +K+ F V +    L   + VS S+ W D  + VRSP+V YS
Sbjct: 641 LQISIEPEILRFGFLEEKKSFVVTISGKELKDGSFVSSSVVWSDGSHSVRSPIVAYS 690

HSP 2 Score: 129.8 bits (325), Expect = 5.1e-30
Identity = 65/104 (62.50%), Postives = 80/104 (76.92%), Query Frame = 1


Query: 81  DVNGHGTHTSSTATGNVITGANLSGLAQGTARGGVPSARLAMYKVCWMSNGCSDMDLLAA 140
           D  GHGTHT+STA GN +  A+  GLAQGTARGGVPSAR+A YKVC+  N C+D+D+LAA
Sbjct: 156 DEEGHGTHTASTAAGNAVQAASFYGLAQGTARGGVPSARIAAYKVCF--NRCNDVDILAA 215

Query: 141 FDAAIQDGVDVISISIAGIGYGNYTDDPISIGAFHAMKKGIITS 185
           FD AI DGVDVISISI+     N  +  ++IG+FHAM +GIIT+
Sbjct: 216 FDDAIADGVDVISISISADYVSNLLNASVAIGSFHAMMRGIITA 257

HSP 3 Score: 89.4 bits (220), Expect = 7.6e-18
Identity = 72/232 (31.03%), Postives = 107/232 (46.12%), Query Frame = 1


Query: 2   VDAKDCMVHSYTNNFNAFAAKLTEAEAKTLSERGDVQHVIPNRYRKLQTTRSWDFLGFPI 61
           + A   +V SY  +FN FAA L++AE++ L    +V  V P++  +L TTRSWDF+GF  
Sbjct: 26  IAASHLLVRSYKRSFNGFAANLSQAESQKLQNMKEVVSVFPSKSHELTTTRSWDFVGFGE 85

Query: 62  NAKRKTRQESDIIVGLFDT---------DVNGHGTHTS----------STATGNVITGAN 121
            A+R++ +ESD+IVG+ D+         D  G G                A  N + GA 
Sbjct: 86  KARRESVKESDVIVGVIDSGIWPESESFDDEGFGPPPKKWKGSCKGGLKFACNNKLIGAR 145

Query: 122 LSGLAQGTARG----GVPSARLAMYKVC------WMSNGCSDMDLLAAFDAAIQ------ 181
                  +AR     G  +A  A            ++ G +   + +A  AA +      
Sbjct: 146 FYNKFADSARDEEGHGTHTASTAAGNAVQAASFYGLAQGTARGGVPSARIAAYKVCFNRC 205

Query: 182 --------------DGVDVISISIAGIGYGNYTDDPISIGAFHAMKKGIITS 185
                         DGVDVISISI+     N  +  ++IG+FHAM +GIIT+
Sbjct: 206 NDVDILAAFDDAIADGVDVISISISADYVSNLLNASVAIGSFHAMMRGIITA 257

[Close]

BLAST of Cucsa.234910 vs. TAIR10
Match: AT5G59100.1 (AT5G59100.1 Subtilisin-like serine endopeptidase family protein)

HSP 1 Score: 241.1 bits (614), Expect = 1.6e-63
Identity = 146/365 (40.00%), Postives = 214/365 (58.63%), Query Frame = 1


Query: 185 LDPIKVKDSLVFCKLMTWGADST-----VKSVGAAGAILQSDQFLDNTDIFMAPSALVSS 244
           LD   VK  +V C       DST      + +GA G+I+++ +  D   I   P + +S+
Sbjct: 387 LDGKLVKGKIVLC-------DSTKGLIEAQKLGAVGSIVKNPE-PDRAFIRSFPVSFLSN 446

Query: 245 FVGATIDAYIHSTRTPTAVIYKTRQ-HRAAAPIIAPFSSRGPNPGSTHILKPDIAAPGVN 304
               ++ +Y++ST+ P A + K+ +     AP++A FSSRGP+   + ILKPDI APGV 
Sbjct: 447 DDYKSLVSYMNSTKNPKATVLKSEEISNQRAPLVASFSSRGPSSIVSDILKPDITAPGVE 506

Query: 305 ILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHVAAAAAYVKSFHPLWSPAAIRSALL 364
           ILA Y+P  S T  + DT+  K++++SGTSMACPHVA  AAYVK+FHP WSP+ I+SA++
Sbjct: 507 ILAAYSPDSSPTESEFDTRRVKYSVLSGTSMACPHVAGVAAYVKTFHPQWSPSMIQSAIM 566

Query: 365 TTAKPISRRGN--PDGEFGYGAGNLNPRKAKNPGLIYDLNEMSYIQFLCREGYSGSSIVI 424
           TTA P++  G+     EF YG+G+++P  A NPGL+Y+L +  +I FLC   Y+   + I
Sbjct: 567 TTAWPMNASGSGFVSTEFAYGSGHVDPIDAINPGLVYELTKADHINFLCGLNYTSDHLRI 626

Query: 425 LTGTKSINCATIIPGQGYDSLNYPTFQLSLQSSREPTTAVFWREVTNVGKPVSVYNATVR 484
           ++G  S  C   I      +LNYPT    + S  +P    F R VTNVG   S YNA V 
Sbjct: 627 ISGDNS-TCTKEISKTLPRNLNYPTMSAKV-SGTKPFNITFQRTVTNVGMQKSTYNAKVV 686

Query: 485 APPG--VEITVEPATLSFSYLHQKERFKVVVKANPLPANTMVSGSITWFDPRYVVRSPVV 540
             PG  + I V P  LS   +++K+ F V V ++ +     VS ++ W D  + VRSP++
Sbjct: 687 KFPGSKLSIKVSPRVLSMKSMNEKQSFMVTVSSDSIGTKQPVSANLIWSDGTHNVRSPII 741

HSP 2 Score: 118.2 bits (295), Expect = 1.5e-26
Identity = 58/103 (56.31%), Postives = 74/103 (71.84%), Query Frame = 1


Query: 81  DVNGHGTHTSSTATGNVITGANLSGLAQGTARGGVPSARLAMYKVCWMSNGCSDMDLLAA 140
           D +GHGTHT+S A GN +  +N  GL  GTARGGVP+AR+A+YKVC  + GC    +++A
Sbjct: 200 DYSGHGTHTASIAAGNAVANSNFYGLGNGTARGGVPAARIAVYKVC-DNEGCDGEAMMSA 259

Query: 141 FDAAIQDGVDVISISIAGIGYGNYTDDPISIGAFHAMKKGIIT 184
           FD AI DGVDVISISI       + +DPI+IGAFHAM  G++T
Sbjct: 260 FDDAIADGVDVISISIVLDNIPPFEEDPIAIGAFHAMAVGVLT 301

HSP 3 Score: 67.8 bits (164), Expect = 2.4e-11
Identity = 36/77 (46.75%), Postives = 48/77 (62.34%), Query Frame = 1


Query: 8   MVHSYTNNFNAFAAKLTEAEAKTLSERGDVQHVIPNRYRKLQTTRSWDFLGFP--INAKR 67
           +V SY  +FN FAA+LTE+E K L+    V  V P+R  KLQTT SW+F+G    I  KR
Sbjct: 71  LVRSYKKSFNGFAARLTESERKRLAGMERVVSVFPSRKLKLQTTSSWNFMGLKEGIKTKR 130

Query: 68  KTRQESDIIVGLFDTDV 83
               ESD I+G+ D+ +
Sbjct: 131 TRSIESDTIIGVIDSGI 147

[Close]

BLAST of Cucsa.234910 vs. TAIR10
Match: AT3G46850.1 (AT3G46850.1 Subtilase family protein)

HSP 1 Score: 239.2 bits (609), Expect = 5.9e-63
Identity = 138/357 (38.66%), Postives = 210/357 (58.82%), Query Frame = 1


Query: 185 LDPIKVKDSLVFCKLMTWGADSTVKSVGAAGAILQSDQFLDNTDIFMAPSALVSSFVGAT 244
           LD  +VK  +V C   T       +++GA  +I+++  + D   +F  P +++S      
Sbjct: 388 LDSKRVKGKIVLCD--TQRNPGEAQAMGAVASIVRNP-YEDAASVFSFPVSVLSEDDYNI 447

Query: 245 IDAYIHSTRTPTAVIYKTRQ-HRAAAPIIAPFSSRGPNPGSTHILKPDIAAPGVNILAGY 304
           + +Y++ST+ P A + K+       AP++A +SSRGPNP    ILKPDI APG  ILA Y
Sbjct: 448 VLSYVNSTKNPKAAVLKSETIFNQKAPVVASYSSRGPNPLIHDILKPDITAPGSEILAAY 507

Query: 305 TPLKSLTGLKGDTQFSKFTLMSGTSMACPHVAAAAAYVKSFHPLWSPAAIRSALLTTAKP 364
           +P   +   + DT+  K+T++SGTSM+CPHVA  AAY+K+FHPLWSP+ I+SA++TTA P
Sbjct: 508 SPY--VPPSESDTRHVKYTVISGTSMSCPHVAGVAAYIKTFHPLWSPSMIQSAIMTTAWP 567

Query: 365 ISRRGNPD---GEFGYGAGNLNPRKAKNPGLIYDLNEMSYIQFLCREGYSGSSIVILTGT 424
           ++   +P     EF YGAG+++P  A +PGL+Y+ N+  +I FLC   Y+G  + +++G 
Sbjct: 568 MNASTSPSNELAEFAYGAGHVDPIAAIHPGLVYEANKSDHITFLCGFNYTGKKLRLISGD 627

Query: 425 KSINCATIIPGQGYDSLNYPTFQLSLQSSREPTTAVFWREVTNVGKPVSVYNATVRAPPG 484
            S +C          +LNYP+    + S  +P    F R VTNVG+P + Y A V     
Sbjct: 628 SS-SCTKEQTKSLTRNLNYPSMSAQV-SGTKPFKVTFRRTVTNVGRPNATYKAKV-VGSK 687

Query: 485 VEITVEPATLSFSYLHQKERFKVVVKANPLPANTMVSGSITWFDPRYVVRSPVVVYS 538
           +++ V PA LS   L++K+ F V V      A  +VS  + W D  + VRSP+VVY+
Sbjct: 688 LKVKVVPAVLSLKSLYEKKSFTVTVSGAGPKAENLVSAQLIWSDGVHFVRSPIVVYA 736

HSP 2 Score: 106.3 bits (264), Expect = 6.0e-23
Identity = 52/104 (50.00%), Postives = 70/104 (67.31%), Query Frame = 1


Query: 81  DVNGHGTHTSSTATGNVITGANLSGLAQGTARGGVPSARLAMYKVCWMS-NGCSDMDLLA 140
           D  GHG+HT+S A GN +   +  GL  GT RGGVP+AR+A+YKVC      C+   +LA
Sbjct: 200 DNTGHGSHTASIAAGNAVKHVSFYGLGNGTVRGGVPAARIAVYKVCDPGVIRCTSDGILA 259

Query: 141 AFDAAIQDGVDVISISIAGIGYGNYTDDPISIGAFHAMKKGIIT 184
           AFD AI D VD+I++S+     G + +D ++IGAFHAM KGI+T
Sbjct: 260 AFDDAIADKVDIITVSLGADAVGTFEEDTLAIGAFHAMAKGILT 303

HSP 3 Score: 64.7 bits (156), Expect = 2.0e-10
Identity = 49/164 (29.88%), Postives = 74/164 (45.12%), Query Frame = 1


Query: 5   KDCMVHSYTNNFNAFAAKLTEAEAKTLSERGDVQHVIPNRYRKLQTTRSWDFLGFP--IN 64
           +D +V +Y  +FN FAA+LTE+E + L+   +V  V P++   LQTT SW+F+G      
Sbjct: 67  QDRLVRNYKRSFNGFAARLTESEREILASMDEVVSVFPSKNLNLQTTTSWNFMGLKEGKR 126

Query: 65  AKRKTRQESDIIVGLFDTDVNGHGTHTSSTATGNVITGANLSGLAQG----TARGGVPSA 124
            KR    ESD I+G+ D+ +       S    G         G+ +G    T    +  A
Sbjct: 127 TKRNPLIESDTIIGVIDSGIYPESDSFSGKGFGP--PPKKWKGVCKGGTNFTCNNKLIGA 186

Query: 125 RLAMYKVCWMSNGCSDMDLLAAFDAAIQDGVDVISISIAGIGYG 163
           R    K+        D     +  A+I  G  V  +S  G+G G
Sbjct: 187 RYYTPKLEGFPESARDNTGHGSHTASIAAGNAVKHVSFYGLGNG 228

[Close]

BLAST of Cucsa.234910 vs. NCBI nr
Match: gi|778730647|ref|XP_004140478.2| (PREDICTED: subtilisin-like protease SBT4.14 [Cucumis sativus])

HSP 1 Score: 710.3 bits (1832), Expect = 2.6e-201
Identity = 352/357 (98.60%), Postives = 355/357 (99.44%), Query Frame = 1


Query: 184 SLDPIKVKDSLVFCKLMTWGADSTVKSVGAAGAILQSDQFLDNTDIFMAPSALVSSFVGA 243
           SLDP KVKDSLVFCKLMTWGADSTVKS+GAAGAILQSDQFLDNTDIFMAPSALVSSFVGA
Sbjct: 354 SLDPSKVKDSLVFCKLMTWGADSTVKSIGAAGAILQSDQFLDNTDIFMAPSALVSSFVGA 413

Query: 244 TIDAYIHSTRTPTAVIYKTRQHRAAAPIIAPFSSRGPNPGSTHILKPDIAAPGVNILAGY 303
           TIDAYIHSTRTPTAVIYKTRQHRAAAPIIAPFSSRGPNPGSTHILKPDIAAPGVNILAGY
Sbjct: 414 TIDAYIHSTRTPTAVIYKTRQHRAAAPIIAPFSSRGPNPGSTHILKPDIAAPGVNILAGY 473

Query: 304 TPLKSLTGLKGDTQFSKFTLMSGTSMACPHVAAAAAYVKSFHPLWSPAAIRSALLTTAKP 363
           TPLKSLTGLKGDTQFSKFTLMSGTSMACPHVAAAAAYVKSFHPLWSPAAIRSALLTTAKP
Sbjct: 474 TPLKSLTGLKGDTQFSKFTLMSGTSMACPHVAAAAAYVKSFHPLWSPAAIRSALLTTAKP 533

Query: 364 ISRRGNPDGEFGYGAGNLNPRKAKNPGLIYDLNEMSYIQFLCREGYSGSSIVILTGTKSI 423
           ISRRGNPDGEFGYGAGNLNPRKAKNPGLIYDLNEMSYIQFLCREGYSGSSI+ILTGTKSI
Sbjct: 534 ISRRGNPDGEFGYGAGNLNPRKAKNPGLIYDLNEMSYIQFLCREGYSGSSIIILTGTKSI 593

Query: 424 NCATIIPGQGYDSLNYPTFQLSLQSSREPTTAVFWREVTNVGKPVSVYNATVRAPPGVEI 483
           NCATIIPG+GYDSLNYPTFQLSLQSSREPTTAVFWREVTNVGKPVSVYNATVRAPPGVEI
Sbjct: 594 NCATIIPGEGYDSLNYPTFQLSLQSSREPTTAVFWREVTNVGKPVSVYNATVRAPPGVEI 653

Query: 484 TVEPATLSFSYLHQKERFKVVVKANPLPANTMVSGSITWFDPRYVVRSPVVVYSPSG 541
           TVEPATLSFSYLHQKERFKVVVKANPLPAN MVSGSITWFDPRYVVRSPVVVYSPSG
Sbjct: 654 TVEPATLSFSYLHQKERFKVVVKANPLPANKMVSGSITWFDPRYVVRSPVVVYSPSG 710

[Close]

BLAST of Cucsa.234910 vs. NCBI nr
Match: gi|778730647|ref|XP_004140478.2| (PREDICTED: subtilisin-like protease SBT4.14 [Cucumis sativus])

HSP 1 Score: 208.4 bits (529), Expect = 3.2e-50
Identity = 103/107 (96.26%), Postives = 103/107 (96.26%), Query Frame = 1


Query: 77  LFDTDVNGHGTHTSSTATGNVITGANLSGLAQGTARGGVPSARLAMYKVCWMSNGCSDMD 136
           L   DVNGHGTHTSSTATGNVITGANLSGLAQGTA GGVPSARLAMYKVCWMSNGCSDMD
Sbjct: 163 LSPVDVNGHGTHTSSTATGNVITGANLSGLAQGTAPGGVPSARLAMYKVCWMSNGCSDMD 222

Query: 137 LLAAFDAAIQDGVDVISISIAGIGYGNYTDDPISIGAFHAMKKGIIT 184
           LLAAFDAAIQDGVDVISISIAGIGYGNYTDDPISIGAFHAMKKGIIT
Sbjct: 223 LLAAFDAAIQDGVDVISISIAGIGYGNYTDDPISIGAFHAMKKGIIT 269

HSP 2 Score: 167.5 bits (423), Expect = 6.2e-38
Identity = 80/82 (97.56%), Postives = 81/82 (98.78%), Query Frame = 1


Query: 1   HVDAKDCMVHSYTNNFNAFAAKLTEAEAKTLSERGDVQHVIPNRYRKLQTTRSWDFLGFP 60
           HVDAKDCMVHSYTNNFNAFAAKLTEAEAKTLSERGDVQHVIPNRYRKLQTTRSWDFLGFP
Sbjct: 28  HVDAKDCMVHSYTNNFNAFAAKLTEAEAKTLSERGDVQHVIPNRYRKLQTTRSWDFLGFP 87

Query: 61  INAKRKTRQESDIIVGLFDTDV 83
           INAKRKTRQESDIIVGLFDT +
Sbjct: 88  INAKRKTRQESDIIVGLFDTGI 109

HSP 3 Score: 666.0 bits (1717), Expect = 5.6e-188
Identity = 330/357 (92.44%), Postives = 341/357 (95.52%), Query Frame = 1


Query: 184 SLDPIKVKDSLVFCKLMTWGADSTVKSVGAAGAILQSDQFLDNTDIFMAPSALVSSFVGA 243
           SLDP KVKDS+VFCKL+TWGADS VKS+GAAGAILQSDQFLDNTDIFMAPS LVSSFVGA
Sbjct: 421 SLDPSKVKDSIVFCKLLTWGADSAVKSIGAAGAILQSDQFLDNTDIFMAPSTLVSSFVGA 480

Query: 244 TIDAYIHSTRTPTAVIYKTRQHRAAAPIIAPFSSRGPNPGSTHILKPDIAAPGVNILAGY 303
           TID+YIHS+RTPTAVIYKTRQH  AAPI+APFSSRGPNPGST ILKPDIAAPGVNILAGY
Sbjct: 481 TIDSYIHSSRTPTAVIYKTRQHIGAAPIVAPFSSRGPNPGSTRILKPDIAAPGVNILAGY 540

Query: 304 TPLKSLTGLKGDTQFSKFTLMSGTSMACPHVAAAAAYVKSFHPLWSPAAIRSALLTTAKP 363
           TPLKSLTGLKGDTQFSKFTLMSGTSMACPHVAAAAAYVKSFHPLWSPAAIRSALLTTAKP
Sbjct: 541 TPLKSLTGLKGDTQFSKFTLMSGTSMACPHVAAAAAYVKSFHPLWSPAAIRSALLTTAKP 600

Query: 364 ISRRGNPDGEFGYGAGNLNPRKAKNPGLIYDLNEMSYIQFLCREGYSGSSIVILTGTKSI 423
           ISRRGNPDGEFGYGAGNLNP KAKNPGLIYDLNEMSYIQFLC EGYSGSSI ILTGTKSI
Sbjct: 601 ISRRGNPDGEFGYGAGNLNPGKAKNPGLIYDLNEMSYIQFLCSEGYSGSSISILTGTKSI 660

Query: 424 NCATIIPGQGYDSLNYPTFQLSLQSSREPTTAVFWREVTNVGKPVSVYNATVRAPPGVEI 483
           NCATII GQGYDSLNYPTFQL LQS+R+PTTAVFWREVTNVG PVSVYN TV APPGVEI
Sbjct: 661 NCATIIRGQGYDSLNYPTFQLRLQSTRQPTTAVFWREVTNVGTPVSVYNTTVWAPPGVEI 720

Query: 484 TVEPATLSFSYLHQKERFKVVVKANPLPANTMVSGSITWFDPRYVVRSPVVVYSPSG 541
           TVEPATLSFSYL QKERFKVVVKANPLP+N MVSGSITWF+PRYVVRSP+VVYSPSG
Sbjct: 721 TVEPATLSFSYLLQKERFKVVVKANPLPSNKMVSGSITWFNPRYVVRSPIVVYSPSG 777

[Close]

BLAST of Cucsa.234910 vs. NCBI nr
Match: gi|659109757|ref|XP_008454861.1| (PREDICTED: xylem serine proteinase 1-like [Cucumis melo])

HSP 1 Score: 203.0 bits (515), Expect = 1.3e-48
Identity = 98/103 (95.15%), Postives = 101/103 (98.06%), Query Frame = 1


Query: 81  DVNGHGTHTSSTATGNVITGANLSGLAQGTARGGVPSARLAMYKVCWMSNGCSDMDLLAA 140
           D+NGHGTHTSSTATGNVITGANLSGLAQGTARGGVPSAR+AMYKVCW S GCSDMDLLAA
Sbjct: 234 DINGHGTHTSSTATGNVITGANLSGLAQGTARGGVPSARVAMYKVCWTSTGCSDMDLLAA 293

Query: 141 FDAAIQDGVDVISISIAGIGYGNYTDDPISIGAFHAMKKGIIT 184
           FDAAIQDGVDVISISIAGIGYGNYT+DPISIGAFHAMKKGIIT
Sbjct: 294 FDAAIQDGVDVISISIAGIGYGNYTEDPISIGAFHAMKKGIIT 336

HSP 2 Score: 158.3 bits (399), Expect = 3.8e-35
Identity = 76/82 (92.68%), Postives = 80/82 (97.56%), Query Frame = 1


Query: 1   HVDAKDCMVHSYTNNFNAFAAKLTEAEAKTLSERGDVQHVIPNRYRKLQTTRSWDFLGFP 60
           HV+AK+ MV+SYTNNFNAFAAKLTEAEAKTLSERGDVQHVIPNRYRKLQTTRSWDFLGFP
Sbjct: 95  HVEAKESMVYSYTNNFNAFAAKLTEAEAKTLSERGDVQHVIPNRYRKLQTTRSWDFLGFP 154

Query: 61  INAKRKTRQESDIIVGLFDTDV 83
           INAKRKTRQESDIIVGLFDT +
Sbjct: 155 INAKRKTRQESDIIVGLFDTGI 176

HSP 3 Score: 564.3 bits (1453), Expect = 2.3e-157
Identity = 278/355 (78.31%), Postives = 310/355 (87.32%), Query Frame = 1


Query: 184 SLDPIKVKDSLVFCKLMTWGADSTVKSVGAAGAILQSDQFLDNTDIFMAPSALVSSFVGA 243
           SLDP KVK SLVFCKL+TWGADS +KSVGA G I+QSDQFLDN DIFMAP+ +VSS VG 
Sbjct: 392 SLDPTKVKGSLVFCKLLTWGADSVIKSVGANGVIIQSDQFLDNADIFMAPATMVSSSVGT 451

Query: 244 TIDAYIHSTRTPTAVIYKTRQHRAAAPIIAPFSSRGPNPGSTHILKPDIAAPGVNILAGY 303
            I  YI STRTPTAVIYKTRQ +A AP++A FSSRGPNPGS  ILKPDIAAPGVNILAGY
Sbjct: 452 IIYTYIKSTRTPTAVIYKTRQLKAKAPMVASFSSRGPNPGSHRILKPDIAAPGVNILAGY 511

Query: 304 TPLKSLTGLKGDTQFSKFTLMSGTSMACPHVAAAAAYVKSFHPLWSPAAIRSALLTTAKP 363
           TPLKSLTG KGDTQ+SKFT+MSGTSMACPHVAAAAAYVKSFHPLWSPAAIRSALLTT +P
Sbjct: 512 TPLKSLTGQKGDTQYSKFTIMSGTSMACPHVAAAAAYVKSFHPLWSPAAIRSALLTTTRP 571

Query: 364 ISRRGNPDGEFGYGAGNLNPRKAKNPGLIYDLNEMSYIQFLCREGYSGSSIVILTGTKSI 423
           ISRR NP+GEF YGAGNLNP KA +PGLIYDLNEMSYIQFLC EGY+GSSI +L GTKSI
Sbjct: 572 ISRRLNPEGEFAYGAGNLNPSKAISPGLIYDLNEMSYIQFLCSEGYTGSSIAVLAGTKSI 631

Query: 424 NCATIIPGQGYDSLNYPTFQLSLQSSREPTTAVFWREVTNVGKPVSVYNATVRAPPGVEI 483
           NC+T+IPG G+DSLNYPTFQLSL+S+++P T  F R+VTNVG+PVSVYNAT++APPGV+I
Sbjct: 632 NCSTLIPGHGHDSLNYPTFQLSLRSTKQPMTTTFRRQVTNVGQPVSVYNATIKAPPGVKI 691

Query: 484 TVEPATLSFSYLHQKERFKVVVKANPLPANTMVSGSITWFDPRYVVRSPVVVYSP 539
           TV P TLSFS L QK  FKVVVKA+PL +  MVSGS+ W   R+VVRSP+VVYSP
Sbjct: 692 TVTPTTLSFSRLLQKRSFKVVVKASPLQSAKMVSGSLAWVGDRHVVRSPIVVYSP 746

[Close]

BLAST of Cucsa.234910 vs. NCBI nr
Match: gi|659109517|ref|XP_008454753.1| (PREDICTED: xylem serine proteinase 1-like [Cucumis melo])

HSP 1 Score: 181.4 bits (459), Expect = 4.2e-42
Identity = 87/107 (81.31%), Postives = 96/107 (89.72%), Query Frame = 1


Query: 77  LFDTDVNGHGTHTSSTATGNVITGANLSGLAQGTARGGVPSARLAMYKVCWMSNGCSDMD 136
           L   D +GHGTHTSSTATGN + GA+LSGLA+GTARGGVPSAR+AMYKVCW S+GCSDMD
Sbjct: 201 LSPVDTDGHGTHTSSTATGNAVAGASLSGLAEGTARGGVPSARVAMYKVCWASSGCSDMD 260

Query: 137 LLAAFDAAIQDGVDVISISIAGIGYGNYTDDPISIGAFHAMKKGIIT 184
           +LAAFDAAIQDGVDVISISI G G+ NY+DD ISIGAFHAMKKGIIT
Sbjct: 261 ILAAFDAAIQDGVDVISISIGGGGFNNYSDDSISIGAFHAMKKGIIT 307

HSP 2 Score: 119.8 bits (299), Expect = 1.5e-23
Identity = 56/82 (68.29%), Postives = 67/82 (81.71%), Query Frame = 1


Query: 1   HVDAKDCMVHSYTNNFNAFAAKLTEAEAKTLSERGDVQHVIPNRYRKLQTTRSWDFLGFP 60
           H +A + MV+SYT +FNAFAAKL++ EAK LS R DV HVIPN YRKLQTTRSWDF+G  
Sbjct: 66  HAEASESMVYSYTKSFNAFAAKLSDHEAKLLSTRKDVHHVIPNTYRKLQTTRSWDFIGLS 125

Query: 61  INAKRKTRQESDIIVGLFDTDV 83
            NA+R+T+ ESDIIVGLFDT +
Sbjct: 126 SNARRRTKHESDIIVGLFDTGI 147

HSP 3 Score: 552.0 bits (1421), Expect = 1.2e-153
Identity = 271/355 (76.34%), Postives = 306/355 (86.20%), Query Frame = 1


Query: 184 SLDPIKVKDSLVFCKLMTWGADSTVKSVGAAGAILQSDQFLDNTDIFMAPSALVSSFVGA 243
           +LDP KVK SLVFCKL+TWGADS +KS+GA G I+QSD+FLDN DIFMAP+ +VSS VG 
Sbjct: 389 TLDPTKVKGSLVFCKLLTWGADSVIKSIGANGVIIQSDEFLDNADIFMAPATMVSSLVGN 448

Query: 244 TIDAYIHSTRTPTAVIYKTRQHRAAAPIIAPFSSRGPNPGSTHILKPDIAAPGVNILAGY 303
            I  YI STRTPTAVIYKT+Q +A AP++A FSSRGPNPGS  ILKPDIAAPGV+ILA Y
Sbjct: 449 IIYTYIKSTRTPTAVIYKTKQLKAKAPMVASFSSRGPNPGSHRILKPDIAAPGVDILAAY 508

Query: 304 TPLKSLTGLKGDTQFSKFTLMSGTSMACPHVAAAAAYVKSFHPLWSPAAIRSALLTTAKP 363
           TPLKSLTG KGDTQ+SKFTLMSGTSMACPHVAAAAAYVKSFHPLWSPAAIRSALLTTA P
Sbjct: 509 TPLKSLTGQKGDTQYSKFTLMSGTSMACPHVAAAAAYVKSFHPLWSPAAIRSALLTTATP 568

Query: 364 ISRRGNPDGEFGYGAGNLNPRKAKNPGLIYDLNEMSYIQFLCREGYSGSSIVILTGTKSI 423
           ISRR NP+GEF YGAGNLNP +A +PGLIYDLNE+SYIQFLC EGY+GSSI +L+GTKSI
Sbjct: 569 ISRRLNPEGEFAYGAGNLNPSRAISPGLIYDLNEISYIQFLCSEGYTGSSIAVLSGTKSI 628

Query: 424 NCATIIPGQGYDSLNYPTFQLSLQSSREPTTAVFWREVTNVGKPVSVYNATVRAPPGVEI 483
           NC+ +IPGQG+DSLNYPTFQLSL+S+ +P T  F R VTNVG P+SVYNAT+ APPGV I
Sbjct: 629 NCSNLIPGQGHDSLNYPTFQLSLKSTNQPMTTTFRRRVTNVGHPISVYNATINAPPGVTI 688

Query: 484 TVEPATLSFSYLHQKERFKVVVKANPLPANTMVSGSITWFDPRYVVRSPVVVYSP 539
           TV P TLSFS L QK  FKVVVKA+PLP+  MVSGS+ W   ++VVRSP+VVYSP
Sbjct: 689 TVTPPTLSFSRLLQKRSFKVVVKASPLPSAKMVSGSLAWVGAQHVVRSPIVVYSP 743

[Close]

BLAST of Cucsa.234910 vs. NCBI nr
Match: gi|778697899|ref|XP_011654432.1| (PREDICTED: subtilisin-like protease SBT4.14 [Cucumis sativus])

HSP 1 Score: 181.8 bits (460), Expect = 3.2e-42
Identity = 88/107 (82.24%), Postives = 96/107 (89.72%), Query Frame = 1


Query: 77  LFDTDVNGHGTHTSSTATGNVITGANLSGLAQGTARGGVPSARLAMYKVCWMSNGCSDMD 136
           L   D +GHGTHTSSTATGN I GA+LSGLA+GTARGGVPSAR+AMYKVCW S+GCSDMD
Sbjct: 198 LSPVDTDGHGTHTSSTATGNAIAGASLSGLAEGTARGGVPSARVAMYKVCWTSSGCSDMD 257

Query: 137 LLAAFDAAIQDGVDVISISIAGIGYGNYTDDPISIGAFHAMKKGIIT 184
           +LAAFDAAIQDGVDVISISI G G+ NY+DD ISIGAFHAMKKGIIT
Sbjct: 258 ILAAFDAAIQDGVDVISISIGGGGFNNYSDDSISIGAFHAMKKGIIT 304

HSP 2 Score: 120.2 bits (300), Expect = 1.1e-23
Identity = 56/82 (68.29%), Postives = 67/82 (81.71%), Query Frame = 1


Query: 1   HVDAKDCMVHSYTNNFNAFAAKLTEAEAKTLSERGDVQHVIPNRYRKLQTTRSWDFLGFP 60
           H +A + MV+SYT +FNAFAAKL++ EAK LS R DV HVIPN+YRKLQTTRSWDF+G  
Sbjct: 63  HAEASESMVYSYTKSFNAFAAKLSDDEAKLLSTRKDVHHVIPNKYRKLQTTRSWDFIGLS 122

Query: 61  INAKRKTRQESDIIVGLFDTDV 83
            NA+R T+ ESDIIVGLFDT +
Sbjct: 123 SNARRSTKHESDIIVGLFDTGI 144

HSP 3 Score: 489.6 bits (1259), Expect = 7.2e-135
Identity = 235/355 (66.20%), Postives = 287/355 (80.85%), Query Frame = 1


Query: 184 SLDPIKVKDSLVFCKLMTWGADSTVKSVGAAGAILQSDQFLDNTDIFMAPSALVSSFVGA 243
           SLDP +VK  +VFC+  +WG DS +K  G  G I+++DQFLD   IFMAP  +V+S VG 
Sbjct: 390 SLDPKRVKGKIVFCQFGSWGTDSVIKGNGGVGTIVENDQFLDAAQIFMAPGTMVNSTVGE 449

Query: 244 TIDAYIHSTRTPTAVIYKTRQHRAAAPIIAPFSSRGPNPGSTHILKPDIAAPGVNILAGY 303
           +IDAYIHS ++P+AVI+K+++ +  AP +A FSSRGPNPGS H+LKPDIAAPG+NILA Y
Sbjct: 450 SIDAYIHSAKSPSAVIFKSQEAQRRAPYVASFSSRGPNPGSEHLLKPDIAAPGINILASY 509

Query: 304 TPLKSLTGLKGDTQFSKFTLMSGTSMACPHVAAAAAYVKSFHPLWSPAAIRSALLTTAKP 363
           T LK+LTGL GDTQ+S+FTLMSGTSMACPHVA  AAYVKSFHP WS AAIRSA+LTTA P
Sbjct: 510 TLLKTLTGLDGDTQYSEFTLMSGTSMACPHVAGVAAYVKSFHPNWSQAAIRSAILTTATP 569

Query: 364 ISRRGNPDGEFGYGAGNLNPRKAKNPGLIYDLNEMSYIQFLCREGYSGSSIVILTGTKSI 423
           +S+R N + EF YG G +NPRKA NPGLIYD++EMSYIQFLC EGYSGSS+  L G+KSI
Sbjct: 570 MSQRVNAEAEFAYGVGQVNPRKAVNPGLIYDMDEMSYIQFLCHEGYSGSSLAPLVGSKSI 629

Query: 424 NCATIIPGQGYDSLNYPTFQLSLQSSREPTTAVFWREVTNVGKPVSVYNATVRAPPGVEI 483
           NC+++IPG GYD++NYPT QLSL+SS++PT AVF R VTNVG   S YNAT+RAP GVEI
Sbjct: 630 NCSSLIPGLGYDAINYPTMQLSLKSSKQPTVAVFRRTVTNVGPASSTYNATIRAPKGVEI 689

Query: 484 TVEPATLSFSYLHQKERFKVVVKANPLPANTMVSGSITWFDPRYVVRSPVVVYSP 539
           TV+P +L+FS+  QK  F+V VKANP+ + TMVSGS+ W   RY VRSP+VVYSP
Sbjct: 690 TVKPMSLTFSHALQKRSFQVEVKANPMDSMTMVSGSLGWKSSRYTVRSPIVVYSP 744

The following BLAST results are available for this feature:

BLAST of Cucsa.234910 vs. Swiss-Prot


Match Name	E-value	Identity	Description
SBT4E_ARATH	1.0e-125	58.33	Subtilisin-like protease SBT4.14 OS=Arabidopsis thaliana GN=SBT4.14 PE=2 SV=1	[more]
SBT4A_ARATH	6.6e-80	38.87	Subtilisin-like protease SBT4.10 OS=Arabidopsis thaliana GN=SBT4.10 PE=5 SV=1	[more]
SBT4F_ARATH	1.6e-78	47.99	Subtilisin-like protease SBT4.15 OS=Arabidopsis thaliana GN=SBT4.15 PE=3 SV=1	[more]
CUCM1_CUCME	1.2e-65	39.44	Cucumisin OS=Cucumis melo PE=1 SV=1	[more]
SBT43_ARATH	1.1e-63	38.66	Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana GN=SBT4.3 PE=3 SV=1	[more]

BLAST of Cucsa.234910 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KME6_CUCSA	8.2e-154	76.34	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G000010 PE=4 SV=1	[more]
A0A0A0KME6_CUCSA	2.2e-42	82.24	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G000010 PE=4 SV=1	[more]
A0A067HDF0_CITSI	2.1e-32	69.90	Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g004503mg PE=4 SV=1	[more]
M5X7G0_PRUPE	2.9e-34	72.82	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa021240mg PE=4 SV=1	[more]
B9GPT5_POPTR	7.2e-33	68.22	Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0002s15330g PE=4 SV=2	[more]

BLAST of Cucsa.234910 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G00230.1	5.8e-127	58.33	xylem serine peptidase 1	[more]
AT5G03620.1	9.1e-80	47.99	Subtilisin-like serine endopeptidase family protein	[more]
AT5G59190.1	6.3e-65	38.66	subtilase family protein	[more]
AT5G59100.1	1.6e-63	40.00	Subtilisin-like serine endopeptidase family protein	[more]
AT3G46850.1	5.9e-63	38.66	Subtilase family protein	[more]

BLAST of Cucsa.234910 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778730647\|ref\|XP_004140478.2\|	2.6e-201	98.60	PREDICTED: subtilisin-like protease SBT4.14 [Cucumis sativus]	[more]
gi\|778730647\|ref\|XP_004140478.2\|	3.2e-50	96.26	PREDICTED: subtilisin-like protease SBT4.14 [Cucumis sativus]	[more]
gi\|659109757\|ref\|XP_008454861.1\|	1.3e-48	95.15	PREDICTED: xylem serine proteinase 1-like [Cucumis melo]	[more]
gi\|659109517\|ref\|XP_008454753.1\|	4.2e-42	81.31	PREDICTED: xylem serine proteinase 1-like [Cucumis melo]	[more]
gi\|778697899\|ref\|XP_011654432.1\|	3.2e-42	82.24	PREDICTED: subtilisin-like protease SBT4.14 [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR000209	Peptidase_S8/S53_dom
IPR010259	S8pro/Inhibitor_I9
IPR015500	Peptidase_S8_subtilisin-rel
IPR023828	Peptidase_S8_Ser-AS

Vocabulary: **Molecular Function**
Term	Definition
GO:0004252	serine-type endopeptidase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006508	proteolysis

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0006508	proteolysis
cellular_component	GO:0005618	cell wall
molecular_function	GO:0032440	2-alkenal reductase [NAD(P)] activity
molecular_function	GO:0004252	serine-type endopeptidase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsa.234910.1	Cucsa.234910.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000209	Peptidase S8/S53 domain	GENE3D	G3DSA:3.40.50.200		coord: 268..386 score: 7.0E-50coord: 78..229 score: 7.0
IPR000209	Peptidase S8/S53 domain	PFAM	PF00082	Peptidase_S8	coord: 81..377 score: 2.2
IPR000209	Peptidase S8/S53 domain	unknown	SSF52743	Subtilisin-like	coord: 48..391 score: 1.06
IPR010259	Peptidase S8 propeptide/proteinase inhibitor I9	GENE3D	G3DSA:3.30.70.80		coord: 10..50 score: 3.
IPR010259	Peptidase S8 propeptide/proteinase inhibitor I9	PFAM	PF05922	Inhibitor_I9	coord: 5..48 score: 6.0
IPR015500	Peptidase S8, subtilisin-related	PRINTS	PR00723	SUBTILISIN	coord: 325..341 score: 2.0E-7coord: 81..94 score: 2.
IPR015500	Peptidase S8, subtilisin-related	PANTHER	PTHR10795	PROPROTEIN CONVERTASE SUBTILISIN/KEXIN	coord: 4..536 score: 5.5E
IPR023828	Peptidase S8, subtilisin, Ser-active site	PROSITE	PS00138	SUBTILASE_SER	coord: 326..336 scor
None	No IPR available	PANTHER	PTHR10795:SF348	SUBFAMILY NOT NAMED	coord: 4..536 score: 5.5E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cucsa.234910	CsaUNG012580	Cucumber (Chinese Long) v2	cgycuB332
Cucsa.234910	MELO3C018314	Melon (DHL92) v3.5.1	cgymeB395
Cucsa.234910	ClCG07G006560	Watermelon (Charleston Gray)	cgywcgB410
Cucsa.234910	CSPI04G27670	Wild cucumber (PI 183967)	cgycpiB347

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:

None