Cucsa.234900 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.234900 vs. Swiss-Prot
Match: SBT11_ARATH (Subtilisin-like protease SBT1.1 OS=Arabidopsis thaliana GN=SBTI1.1 PE=1 SV=1)

HSP 1 Score: 841.3 bits (2172), Expect = 8.6e-243
Identity = 426/767 (55.54%), Postives = 542/767 (70.66%), Query Frame = 1

Query: 1   MGFREVWVLLSIMLAVSSAVVDQQTYIIHMDATKMVTPIPEQWYTDIIDSVNKLSSLDDN 60
           M FR   V   ++   S+    +QTY+IH      VT   +   T + +S+   +  DD+
Sbjct: 19  MFFRSFIVFFFLIFFASNVSSRKQTYVIHT-----VTTSTKHIVTSLFNSLQTENINDDD 78

Query: 61  EEEASNAAEILYVYKTALSGFAAKLTSKKLHSLSKIPGFLAATPNELLQLHTTHSPQFLG 120
                +  EI Y+Y+ A+SGF+A LT  +L ++    GF++A P+ELL LHTT+S +FLG
Sbjct: 79  ----FSLPEIHYIYENAMSGFSATLTDDQLDTVKNTKGFISAYPDELLSLHTTYSHEFLG 138

Query: 121 LQRDHGLWNSSNLASDIIIGLLDTGVWPEHISFQDESLSSVPLKWKGICQTGPRFSSSNC 180
           L+   GLWN ++L+SD+IIGL+DTG+ PEH+SF+D  ++ VP +W+G C  G  FSSS C
Sbjct: 139 LEFGIGLWNETSLSSDVIIGLVDTGISPEHVSFRDTHMTPVPSRWRGSCDEGTNFSSSEC 198

Query: 181 NKKLIGASFYIKGYEAIVGRLNETGIFRSPRDSNGHGTHTASTAAGSIVNNASFFNQGMG 240
           NKK+IGAS + KGYE+IVG++NET  FRS RD+ GHGTHTASTAAG IV  A++F Q  G
Sbjct: 199 NKKIIGASAFYKGYESIVGKINETTDFRSTRDAQGHGTHTASTAAGDIVPKANYFGQAKG 258

Query: 241 VASGIRFTSRIVAYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGGSSSFYKDNIAI 300
           +ASG+RFTSRI AYK CW LGCA+ D++AA+D A+ DGVDV+SLSLGG S  FY D IAI
Sbjct: 259 LASGMRFTSRIAAYKACWALGCASTDVIAAIDRAILDGVDVISLSLGGSSRPFYVDPIAI 318

Query: 301 AAFGAIEKGVFVSCSAGNSGPSPSTVGNAAPWIMTVAASYTDRTFPTTVKLGNGQVFEGS 360
           A FGA++K +FVSCSAGNSGP+ STV N APW+MTVAASYTDRTFP  V++GN +   GS
Sbjct: 319 AGFGAMQKNIFVSCSAGNSGPTASTVSNGAPWLMTVAASYTDRTFPAIVRIGNRKSLVGS 378

Query: 361 SLYYGKSINELPLVYNNTAGDGQETNFCIAGSLDPSMVKGKIVVCERGQISRTEKGEQVK 420
           SLY GKS+  LPL +N TAG+     FCI  SL   +V+GKIV+C RG   RT KGE+VK
Sbjct: 379 SLYKGKSLKNLPLAFNRTAGEESGAVFCIRDSLKRELVEGKIVICLRGASGRTAKGEEVK 438

Query: 421 LAGGAGMILINTEFEGEELFADPHILPATTLGALAGKAILDYTASSKTQAKALIVFEGTK 480
            +GGA M+L++TE EGEEL ADPH+LPA +LG   GK +L+Y A +   A A + F GT 
Sbjct: 439 RSGGAAMLLVSTEAEGEELLADPHVLPAVSLGFSDGKTLLNYLAGA-ANATASVRFRGTA 498

Query: 481 YGSQAPRVAAFSSRGPSLVGPDVIKPDVTAPGVNILAAWPPIVSPSELESDTRRVLFNII 540
           YG+ AP VAAFSSRGPS+ GP++ KPD+ APG+NILA W P  SPS L SD RRV FNII
Sbjct: 499 YGATAPMVAAFSSRGPSVAGPEIAKPDIAAPGLNILAGWSPFSSPSLLRSDPRRVQFNII 558

Query: 541 SGTSMSCPHVSGLAALLKSAHNDWSPAAIKSALMTTAYITDNKMSLISDVGQANGE-PAT 600
           SGTSM+CPH+SG+AAL+KS H DWSPA IKSA+MTTA ITDN+   I D G A  E  AT
Sbjct: 559 SGTSMACPHISGIAALIKSVHGDWSPAMIKSAIMTTARITDNRNRPIGDRGAAGAESAAT 618

Query: 601 PFTFGSGHVDPEKASDPGLIYDITPQDYINYLCSLKYNSTQIALVSRGNFTCSSKRTVVK 660
            F FG+G+VDP +A DPGL+YD +  DY+NYLCSL Y S +I L S  N+TC+S   V+ 
Sbjct: 619 AFAFGAGNVDPTRAVDPGLVYDTSTVDYLNYLCSLNYTSERILLFSGTNYTCASNAVVLS 678

Query: 661 PGDLNYPSFSVFMKKKAKKVSITLKRTVTNVGISRSDYTVKINNPKGITVIVKPEKLSFG 720
           PGDLNYPSF+V +   A   ++  KRTVTNVG    +Y V +  PKG+ V V+P+ L F 
Sbjct: 679 PGDLNYPSFAVNLVNGANLKTVRYKRTVTNVGSPTCEYMVHVEEPKGVKVRVEPKVLKFQ 738

Query: 721 SLGEQLSYQVRFVSLGGKEALDTFSFGSLVWISGKYAVRSPIAVTWQ 767
              E+LSY V + +   + +  + SFG LVWI  KY VRSPIAVTW+
Sbjct: 739 KARERLSYTVTYDAEASRNS-SSSSFGVLVWICDKYNVRSPIAVTWE 774

BLAST of Cucsa.234900 vs. Swiss-Prot
Match: SBT13_ARATH (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana GN=SBT1.3 PE=2 SV=1)

HSP 1 Score: 705.7 bits (1820), Expect = 5.6e-202
Identity = 369/769 (47.98%), Postives = 504/769 (65.54%), Query Frame = 1

Query: 9   LLSIMLAVSSAVVDQQTYIIHMDATKMVTPIPE--QWYTDIIDSVNKLSSLDDNEEEASN 68
           L+ +    ++ +  ++TY+IHMD + M  P     QWY+  I+SV +  S    +EE  N
Sbjct: 20  LIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKS----QEEEGN 79

Query: 69  AAEILYVYKTALSGFAAKLTSKKLHSLSKIPGFLAATPNELLQLHTTHSPQFLGLQRDHG 128
              ILY Y+TA  G AA+LT ++   L +  G +A  P    +LHTT SP FLGL+R   
Sbjct: 80  NNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLERQES 139

Query: 129 --LWNSSNLASDIIIGLLDTGVWPEHISFQDESLSSVPLKWKGICQTGPRFSSSNCNKKL 188
             +W       D+++G+LDTG+WPE  SF D  +S VP  W+G C+TG RF   NCN+K+
Sbjct: 140 ERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNCNRKI 199

Query: 189 IGASFYIKGYEAIVGRLNETGIFRSPRDSNGHGTHTASTAAGSIVNNASFFNQGMGVASG 248
           +GA  + +GYEA  G+++E   ++SPRD +GHGTHTA+T AGS V  A+ F    G A G
Sbjct: 200 VGARVFYRGYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYGTARG 259

Query: 249 IRFTSRIVAYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGGSSSFYKDNIAIAAFG 308
           +   +R+ AYKVCW  GC ++DIL+A+D AVADGV VLS+SLGGG S++ +D+++IA FG
Sbjct: 260 MAQKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRDSLSIATFG 319

Query: 309 AIEKGVFVSCSAGNSGPSPSTVGNAAPWIMTVAASYTDRTFPTTVKLGNGQVFEGSSLYY 368
           A+E GVFVSCSAGN GP P ++ N +PWI TV AS  DR FP TVK+G  + F+G SLY 
Sbjct: 320 AMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFKGVSLYK 379

Query: 369 GKSI----NELPLVY-NNTAGDGQETNFCIAGSLDPSMVKGKIVVCERGQISRTEKGEQV 428
           G+++     + PLVY    A     T+FC+ G+LD   V GKIV+C+RG   R +KG+ V
Sbjct: 380 GRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRVQKGQVV 439

Query: 429 KLAGGAGMILINTEFEGEELFADPHILPATTLGALAGKAILDYTASSKTQAKALIVFEGT 488
           K AGG GM+L NT   GEEL AD H+LPA  +G   GK I  Y  +SK +A A +   GT
Sbjct: 440 KRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSK-KATASLEILGT 499

Query: 489 KYG-SQAPRVAAFSSRGPSLVGPDVIKPDVTAPGVNILAAWPPIVSPSELESDTRRVLFN 548
           + G   +P VAAFSSRGP+ +  +++KPD+ APGVNILAAW   ++PS L SD RRV FN
Sbjct: 500 RIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPRRVKFN 559

Query: 549 IISGTSMSCPHVSGLAALLKSAHNDWSPAAIKSALMTTAYITDNKMSLISDVGQANGEPA 608
           I+SGTSMSCPHVSG+AAL+KS H DWSPAAIKSALMTTAY+ DN    ++D   A   P+
Sbjct: 560 ILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGA--APS 619

Query: 609 TPFTFGSGHVDPEKASDPGLIYDITPQDYINYLCSLKYNSTQIALVSR-GNFTCSSKRTV 668
           +P+  G+GH+DP +A+DPGL+YDI PQ+Y  +LC+   + +Q+ + ++  N TC  K T+
Sbjct: 620 SPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTC--KHTL 679

Query: 669 VK-PGDLNYPSFSVFMKKKAKKVSITLKRTVTNVGISRSDYTVKINNPKGITVIVKPEKL 728
            K PG+LNYP+ S    +     ++TL+RTVTNVG   S Y V ++  KG +V V+P+ L
Sbjct: 680 AKNPGNLNYPAISALFPENTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGASVTVQPKTL 739

Query: 729 SFGSLGEQLSYQVRFVSLGGKEALDTFSFGSLVWISGKYAVRSPIAVTW 766
           +F S  ++LSY V F +   +  +    FG LVW S  + VRSP+ +TW
Sbjct: 740 NFTSKHQKLSYTVTFRT---RFRMKRPEFGGLVWKSTTHKVRSPVIITW 776

BLAST of Cucsa.234900 vs. Swiss-Prot
Match: SBT15_ARATH (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 699.1 bits (1803), Expect = 5.3e-200
Identity = 382/768 (49.74%), Postives = 493/768 (64.19%), Query Frame = 1

Query: 17  SSAVVDQQTYIIHMD--ATKMVTPIPEQWYTDIIDSVNKLSSLDDNEEEASNAAEILYVY 76
           S++  +  TYI+H+D  A   + P    WYT  + S+             S+   I++ Y
Sbjct: 19  SASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLT------------SSPPSIIHTY 78

Query: 77  KTALSGFAAKLTSKKLHSLSKIPGFLAATPNELLQLHTTHSPQFLGLQRDH--GLWNSSN 136
            T   GF+A+LTS+    L   P  ++  P ++  LHTT SP+FLGL+     GL   S+
Sbjct: 79  DTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEESD 138

Query: 137 LASDIIIGLLDTGVWPEHISFQDESLSSVPLKWKGICQTGPRFSSSNCNKKLIGASFYIK 196
             SD++IG++DTGVWPE  SF D  L  VP+KWKG C     F  S CN+KL+GA F+  
Sbjct: 139 FGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCG 198

Query: 197 GYEAIVGRLNETGIFRSPRDSNGHGTHTASTAAGSIVNNASFFNQGMGVASGIRFTSRIV 256
           GYEA  G++NET  FRSPRDS+GHGTHTAS +AG  V  AS      GVA+G+   +R+ 
Sbjct: 199 GYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLA 258

Query: 257 AYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGGSSSFYKDNIAIAAFGAIEKGVFV 316
           AYKVCW  GC ++DILAA D+AVADGVDV+SLS+GG    +Y D IAI AFGAI++G+FV
Sbjct: 259 AYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFV 318

Query: 317 SCSAGNSGPSPSTVGNAAPWIMTVAASYTDRTFPTTVKLGNGQVFEGSSLYYGKSINE-- 376
           S SAGN GP   TV N APW+ TV A   DR FP  VKLGNG++  G S+Y G  ++   
Sbjct: 319 SASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDPGR 378

Query: 377 -LPLVYNNT--AGDGQETNFCIAGSLDPSMVKGKIVVCERGQISRTEKGEQVKLAGGAGM 436
             PLVY  +   GDG  ++ C+ GSLDP++VKGKIV+C+RG  SR  KGE V+  GG GM
Sbjct: 379 MYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKNGGLGM 438

Query: 437 ILINTEFEGEELFADPHILPATTLGALAGKAILDY-TASSKTQAK----ALIVFEGTKYG 496
           I+ N  F+GE L AD H+LPAT++GA  G  I  Y + SSK+++     A IVF+GT+ G
Sbjct: 439 IIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLG 498

Query: 497 SQ-APRVAAFSSRGPSLVGPDVIKPDVTAPGVNILAAWPPIVSPSELESDTRRVLFNIIS 556
            + AP VA+FS+RGP+   P+++KPDV APG+NILAAWP  + PS + SD RR  FNI+S
Sbjct: 499 IRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILS 558

Query: 557 GTSMSCPHVSGLAALLKSAHNDWSPAAIKSALMTTAYITDNKMSLISDVGQANGEPATPF 616
           GTSM+CPHVSGLAALLK+AH DWSPAAI+SAL+TTAY  DN    + D  ++ G  ++  
Sbjct: 559 GTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMD--ESTGNTSSVM 618

Query: 617 TFGSGHVDPEKASDPGLIYDITPQDYINYLCSLKYNSTQIALVSRGNFTCSSKRTVVKPG 676
            +GSGHV P KA DPGL+YDIT  DYIN+LC+  Y  T I  ++R    C   R     G
Sbjct: 619 DYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAGHVG 678

Query: 677 DLNYPSFS-VFMKKKAKKVSITLKRTVTNVGISRSDYTVKINNPKGITVIVKPEKLSFGS 736
           +LNYPSFS VF +    K+S    RTVTNVG S S Y +KI  P+G TV V+PEKLSF  
Sbjct: 679 NLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFRR 738

Query: 737 LGEQLSYQVRFVSLGGK--EALDTFSFGSLVWISGKYAVRSPIAVTWQ 767
           +G++LS+ VR  +   K          G +VW  GK  V SP+ VT Q
Sbjct: 739 VGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVTLQ 772

BLAST of Cucsa.234900 vs. Swiss-Prot
Match: SBT17_ARATH (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 688.3 bits (1775), Expect = 9.3e-197
Identity = 362/757 (47.82%), Postives = 492/757 (64.99%), Query Frame = 1

Query: 16  VSSAVVDQQTYIIHMDATKMVTP--IPEQWYTDIIDSVNKLSSLDDNEEEASNAAEILYV 75
           VSS+  DQ TYI+HM  ++M +   +   WY   + S+             S++AE+LY 
Sbjct: 22  VSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSI-------------SDSAELLYT 81

Query: 76  YKTALSGFAAKLTSKKLHSLSKIPGFLAATPNELLQLHTTHSPQFLGL-QRDHGLWNSSN 135
           Y+ A+ GF+ +LT ++  SL   PG ++  P    +LHTT +P FLGL +    L+  + 
Sbjct: 82  YENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEAG 141

Query: 136 LASDIIIGLLDTGVWPEHISFQDESLSSVPLKWKGICQTGPRFSSSNCNKKLIGASFYIK 195
             SD+++G+LDTGVWPE  S+ DE    +P  WKG C+ G  F++S CN+KLIGA F+ +
Sbjct: 142 SYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFAR 201

Query: 196 GYEAIVGRLNETGIFRSPRDSNGHGTHTASTAAGSIVNNASFFNQGMGVASGIRFTSRIV 255
           GYE+ +G ++E+   RSPRD +GHGTHT+STAAGS+V  AS      G A G+   +R+ 
Sbjct: 202 GYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVA 261

Query: 256 AYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGGSSSFYKDNIAIAAFGAIEKGVFV 315
            YKVCW  GC ++DILAA+D A+AD V+VLS+SLGGG S +Y+D +AI AF A+E+G+ V
Sbjct: 262 VYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILV 321

Query: 316 SCSAGNSGPSPSTVGNAAPWIMTVAASYTDRTFPTTVKLGNGQVFEGSSLYYGKSINE-- 375
           SCSAGN+GPS S++ N APWI TV A   DR FP    LGNG+ F G SL+ G+++ +  
Sbjct: 322 SCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDKL 381

Query: 376 LPLVYNNTAGDGQETNFCIAGSLDPSMVKGKIVVCERGQISRTEKGEQVKLAGGAGMILI 435
           LP +Y   A +    N C+ G+L P  VKGKIV+C+RG  +R +KG+ VK AGG GMIL 
Sbjct: 382 LPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILA 441

Query: 436 NTEFEGEELFADPHILPATTLGALAGKAILDYTASSKTQAKALIVFEGTKYG-SQAPRVA 495
           NT   GEEL AD H+LPATT+G  AG  I  Y  +      ++ +  GT  G   +P VA
Sbjct: 442 NTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISIL-GTVVGVKPSPVVA 501

Query: 496 AFSSRGPSLVGPDVIKPDVTAPGVNILAAWPPIVSPSELESDTRRVLFNIISGTSMSCPH 555
           AFSSRGP+ + P+++KPD+ APGVNILAAW     P+ L SD+RRV FNIISGTSMSCPH
Sbjct: 502 AFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPH 561

Query: 556 VSGLAALLKSAHNDWSPAAIKSALMTTAYITDNKMSLISDVGQANGEPATPFTFGSGHVD 615
           VSGLAALLKS H +WSPAAI+SALMTTAY T      + D+  A G+P+TPF  G+GHV 
Sbjct: 562 VSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDI--ATGKPSTPFDHGAGHVS 621

Query: 616 PEKASDPGLIYDITPQDYINYLCSLKYNSTQIALVSRGNFTCSSKRTVVKPGDLNYPSFS 675
           P  A++PGLIYD+T +DY+ +LC+L Y S QI  VSR N+TC   ++     DLNYPSF+
Sbjct: 622 PTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKS-YSVADLNYPSFA 681

Query: 676 VFMKKKAKKVSITLKRTVTNVGISRSDYTVKI-NNPKGITVIVKPEKLSFGSLGEQLSYQ 735
           V +       +    RTVT+VG     Y+VK+ +   G+ + V+P  L+F    E+ SY 
Sbjct: 682 VNVDGVG---AYKYTRTVTSVG-GAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYT 741

Query: 736 VRFVSLGGKEALDTFSFGSLVWISGKYAVRSPIAVTW 766
           V F ++   +   + SFGS+ W  GK+ V SP+A++W
Sbjct: 742 VTF-TVDSSKPSGSNSFGSIEWSDGKHVVGSPVAISW 756

BLAST of Cucsa.234900 vs. Swiss-Prot
Match: SBT18_ARATH (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana GN=SBT1.8 PE=2 SV=1)

HSP 1 Score: 671.0 bits (1730), Expect = 1.5e-191
Identity = 353/707 (49.93%), Postives = 459/707 (64.92%), Query Frame = 1

Query: 63  EASNAAEILYVYKTALSGFAAKLTSKKLHSL-SKIPGFLAATPNELLQLHTTHSPQFLGL 122
           + ++ + +LY Y T+  GF+A L S +  SL S     L    + L  LHTT +P+FLGL
Sbjct: 53  QLNSESSLLYTYTTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGL 112

Query: 123 QRDHGLWNSSNLASDIIIGLLDTGVWPEHISFQDESLSSVPLKWKGICQTGPRFSSSNCN 182
             + G+ +  + ++ +IIG+LDTGVWPE  SF D  +  +P KWKG C++G  F S  CN
Sbjct: 113 NSEFGVHDLGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCN 172

Query: 183 KKLIGASFYIKGYE-AIVGRLNETGIFRSPRDSNGHGTHTASTAAGSIVNNASFFNQGMG 242
           KKLIGA  + KG++ A  G  +      SPRD +GHGTHT++TAAGS V NASF     G
Sbjct: 173 KKLIGARSFSKGFQMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAG 232

Query: 243 VASGIRFTSRIVAYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGGSSSFYKDNIAI 302
            A G+   +R+  YKVCW  GC  +DILAAMD A+ DGVDVLSLSLGGGS+ +Y+D IAI
Sbjct: 233 TARGMATRARVATYKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAI 292

Query: 303 AAFGAIEKGVFVSCSAGNSGPSPSTVGNAAPWIMTVAASYTDRTFPTTVKLGNGQVFEGS 362
            AF A+E+GVFVSCSAGNSGP+ ++V N APW+MTV A   DR FP    LGNG+   G 
Sbjct: 293 GAFSAMERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGV 352

Query: 363 SLYYGKSINELPLVYNNTAGDGQETNFCIAGSLDPSMVKGKIVVCERGQISRTEKGEQVK 422
           SLY G  +   PL      G+   +N C+ GSLD S+V+GKIVVC+RG  +R EKG  V+
Sbjct: 353 SLYSGVGMGTKPLELVYNKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVR 412

Query: 423 LAGGAGMILINTEFEGEELFADPHILPATTLGALAGKAILDYTASSKTQAKALIVFEGTK 482
            AGG GMI+ NT   GEEL AD H+LPA  +G   G  + +Y   S ++  AL+VF+GT 
Sbjct: 413 DAGGLGMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREY-VKSDSKPTALLVFKGTV 472

Query: 483 YG-SQAPRVAAFSSRGPSLVGPDVIKPDVTAPGVNILAAWPPIVSPSELESDTRRVLFNI 542
                +P VAAFSSRGP+ V P+++KPDV  PGVNILA W   + P+ L+ D+RR  FNI
Sbjct: 473 LDVKPSPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNI 532

Query: 543 ISGTSMSCPHVSGLAALLKSAHNDWSPAAIKSALMTTAYITDNKMSLISDVGQANGEPAT 602
           +SGTSMSCPH+SGLA LLK+AH +WSP+AIKSALMTTAY+ DN  + + D   A+   + 
Sbjct: 533 MSGTSMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDA--ADNSLSN 592

Query: 603 PFTFGSGHVDPEKASDPGLIYDITPQDYINYLCSLKYNSTQI-ALVSRGNFTCSSKRTVV 662
           P+  GSGHVDP+KA  PGL+YDI+ ++YI +LCSL Y    I A+V R +  CS K +  
Sbjct: 593 PYAHGSGHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFS-- 652

Query: 663 KPGDLNYPSFSVFMKKKAKKVSITLKRTVTNVGISRSDYTVKINNPKGITVIVKPEKLSF 722
            PG LNYPSFSV       K  +   R VTNVG + S Y V +N    + + VKP KLSF
Sbjct: 653 DPGQLNYPSFSVLF---GGKRVVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSF 712

Query: 723 GSLGEQLSYQVRFVSLGGKEALDTFSFGSLVWISGKYAVRSPIAVTW 766
            S+GE+  Y V FVS  G    +   FGS+ W + ++ VRSP+A +W
Sbjct: 713 KSVGEKKRYTVTFVSKKGVSMTNKAEFGSITWSNPQHEVRSPVAFSW 751

BLAST of Cucsa.234900 vs. TrEMBL
Match: B9IC48_POPTR (Subtilase family protein OS=Populus trichocarpa GN=POPTR_0014s07060g PE=3 SV=2)

HSP 1 Score: 996.1 bits (2574), Expect = 2.3e-287
Identity = 497/772 (64.38%), Postives = 602/772 (77.98%), Query Frame = 1

Query: 1   MGFREVWVLLSIMLAVSSAV-VDQQTYIIHMDATKMVTPIP-----EQWYTDIIDSVNKL 60
           M FR   +LL+ M A +SA  +D+QTYIIHMD  KM           QWY  +IDS+ + 
Sbjct: 1   MIFRISLLLLAFMAAKASAASIDKQTYIIHMDKNKMPALYDFLGNSRQWYESVIDSITQF 60

Query: 61  SSLDDNEEEASNAAEILYVYKTALSGFAAKLTSKKLHSLSKIPGFLAATPNELLQLHTTH 120
           SS +  EE  +   ++LY Y+T  SGFAAKL++K++ +LS++ GFL+A P+ +L LHTTH
Sbjct: 61  SSQEHEEEHETGFPQLLYTYETVTSGFAAKLSTKQVEALSRVDGFLSAIPDGMLTLHTTH 120

Query: 121 SPQFLGLQRDHGLWNSSNLASDIIIGLLDTGVWPEHISFQDESLSSVPLKWKGICQTGPR 180
           +P+FLGLQ   GLWN+ NLASD+I+G+LDTG+WPEH+SFQD  +S+VPLKWKG C++G +
Sbjct: 121 TPRFLGLQSGKGLWNAQNLASDVIVGILDTGIWPEHVSFQDSGMSAVPLKWKGKCESGTK 180

Query: 181 FSSSNCNKKLIGASFYIKGYEAIVGRLNETGIFRSPRDSNGHGTHTASTAAGSIVNNASF 240
           FS SNCNKKLIGA  + KGYE+IVGR+NET  +RSPRDS GHGTHTA+TAAG++V+ ASF
Sbjct: 181 FSPSNCNKKLIGARAFFKGYESIVGRINETIDYRSPRDSQGHGTHTAATAAGNLVDEASF 240

Query: 241 FNQGMGVASGIRFTSRIVAYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGGSSSFY 300
           +    G A+G+++T+RI AYKVCW  GC N D+LAA+D AVADGVDVLSLSLGG +  FY
Sbjct: 241 YGLANGSAAGMKYTARIAAYKVCWTSGCTNTDLLAAIDQAVADGVDVLSLSLGGSAKPFY 300

Query: 301 KDNIAIAAFGAIEKGVFVSCSAGNSGPSPSTVGNAAPWIMTVAASYTDRTFPTTVKLGNG 360
            D++AIA+FGAI+KGVFVSCSAGNSGPS S+V N APWIMTVAASYTDR FPTTVKLGNG
Sbjct: 301 SDSVAIASFGAIQKGVFVSCSAGNSGPSISSVDNNAPWIMTVAASYTDRRFPTTVKLGNG 360

Query: 361 QVFEGSSLYYGKSINELPLVYNNTAGDGQETNFCIAGSLDPSMVKGKIVVCERGQISRTE 420
           Q FEG+SLY GK+  +LPLVY  TAG G+   +CI GSL   +VKGK+VVC+RG   R E
Sbjct: 361 QTFEGASLYTGKATAQLPLVYAGTAG-GEGAEYCIIGSLKKKLVKGKMVVCKRGMNGRAE 420

Query: 421 KGEQVKLAGGAGMILINTEFEGEELFADPHILPATTLGALAGKAILDYTASSKTQAKALI 480
           KGEQVKLAGG GM+LINTE  GEELFAD H LPAT+LGA AG A+ +Y  S+K +A A I
Sbjct: 421 KGEQVKLAGGTGMLLINTETGGEELFADAHFLPATSLGASAGIAVKEYMNSTK-RATASI 480

Query: 481 VFEGTKYGSQAPRVAAFSSRGPSLVGPDVIKPDVTAPGVNILAAWPPIVSPSELESDTRR 540
            F+GT YG+ AP +AAFSSRGPS VGPDVIKPDVTAPGVNILAAWPP+ SP+ L+SD R 
Sbjct: 481 AFKGTVYGNPAPMLAAFSSRGPSSVGPDVIKPDVTAPGVNILAAWPPMTSPTLLKSDKRS 540

Query: 541 VLFNIISGTSMSCPHVSGLAALLKSAHNDWSPAAIKSALMTTAYITDNKMSLISDVGQAN 600
           VLFN+ISGTSMSCPHVSGLAALLKS H  WSPAAIKSALMTTAY+TDN+ S I+D G +N
Sbjct: 541 VLFNVISGTSMSCPHVSGLAALLKSVHKTWSPAAIKSALMTTAYVTDNRGSPIADAGSSN 600

Query: 601 GEPATPFTFGSGHVDPEKASDPGLIYDITPQDYINYLCSLKYNSTQIALVSRGNFTCSSK 660
              ATPF FGSGHVDPE ASDPGLIYDIT +DY+NY CSL Y S+QIA VSR N TC   
Sbjct: 601 SASATPFAFGSGHVDPESASDPGLIYDITIEDYLNYFCSLNYTSSQIAQVSRRNVTCPDN 660

Query: 661 RTVVKPGDLNYPSFSVFMKKKAKKVSITLKRTVTNVGISRSDYTVKINNPKGITVIVKPE 720
           +  ++PGDLNYPSF+V  +  A+   +  KRT+TNVG   S Y VK+  P G++VI++P+
Sbjct: 661 K-ALQPGDLNYPSFAVNFEGNARNNRVKYKRTLTNVGTPWSTYAVKVEEPNGVSVILEPK 720

Query: 721 KLSFGSLGEQLSYQVRFVSLGGKEALDTFSFGSLVWISGKYAVRSPIAVTWQ 767
            LSF  LG++LSY V FVS  GK    + SFGSLVW+SGKY+VRSPIAVTWQ
Sbjct: 721 SLSFEKLGQKLSYNVTFVSSRGKGREGSSSFGSLVWLSGKYSVRSPIAVTWQ 769

BLAST of Cucsa.234900 vs. TrEMBL
Match: M5X6Y7_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa027143mg PE=3 SV=1)

HSP 1 Score: 992.6 bits (2565), Expect = 2.6e-286
Identity = 496/758 (65.44%), Postives = 600/758 (79.16%), Query Frame = 1

Query: 10  LSIMLAVSSAVVDQQTYIIHMDATKMVTPIPEQWYTDIIDSVNKLSSLDDNEEEASNAAE 69
           L IM     A++++QTYIIHMD TK+     +Q+Y  +IDS+ KLSS ++ EE  +   +
Sbjct: 11  LVIMATTKIALMEEQTYIIHMDKTKITDSDHQQYYQAVIDSITKLSSQEEEEENKTPTPQ 70

Query: 70  ILYVYKTALSGFAAKLTSKKLHSLSKIPGFLAATPNELLQLHTTHSPQFLGLQRDHGLWN 129
           +LY+Y+TA+SGFAAKL++ +L SL+++ GFL ATP+ELL LHTTH+PQFLGLQ   GLW+
Sbjct: 71  LLYIYETAISGFAAKLSTNQLKSLNQVDGFLFATPDELLSLHTTHTPQFLGLQNGKGLWS 130

Query: 130 SSNLASDIIIGLLDTGVWPEHISFQDESLSSVPLKWKGICQTGPRFSSSNCNKKLIGASF 189
           +SN ASD+I+GL+DTG+WPEH+SFQD  +S VP +WKG C+ G RFS SNCNKKLIGA  
Sbjct: 131 ASNSASDVIVGLVDTGIWPEHVSFQDSGMSRVPSRWKGTCEEGTRFSFSNCNKKLIGARA 190

Query: 190 YIKGYEAIVGRLNETGIFRSPRDSNGHGTHTASTAAGSIVNNASFFNQGMGVASGIRFTS 249
           +++GYEAIVGR+NET  +RSPRDSNGHGTHTASTAAG+ VN AS F    G ASG+++T+
Sbjct: 191 FVQGYEAIVGRVNETVDYRSPRDSNGHGTHTASTAAGNFVNQASLFGLAKGSASGMKYTA 250

Query: 250 RIVAYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGGSSSFYKDNIAIAAFGAIEKG 309
           RI AYK CW LGCAN+D++AA++SAVADGVD+LSLSLGG S  +YKDNIAIA+FGAI+ G
Sbjct: 251 RIAAYKACWTLGCANSDVMAAIESAVADGVDILSLSLGGVSKPYYKDNIAIASFGAIQHG 310

Query: 310 VFVSCSAGNSGPSPSTVGNAAPWIMTVAASYTDRTFPTTVKLGNGQVFEGSSLYYGKSIN 369
           V VSCSAGNSGPS S+V NAAPWIMTVAASY+DR+FPT VKLG+GQ+FEGSSLY GK   
Sbjct: 311 VSVSCSAGNSGPSRSSVSNAAPWIMTVAASYSDRSFPTAVKLGDGQIFEGSSLYSGKKTK 370

Query: 370 ELPLVYNNTAGDGQETNFCIAGSLDPSMVKGKIVVCERGQISRTEKGEQVKLAGGAGMIL 429
           +LPLVYN TAG  Q   +C  GSL   +VKGKIVVCE G  S+T  GE+VK AGGAGM+L
Sbjct: 371 QLPLVYNRTAG-SQGAEYCFEGSLVKKLVKGKIVVCEGGIYSQTGVGEKVKKAGGAGMLL 430

Query: 430 INTEFEGEELFADPHILPATTLGALAGKAILDYTASSKTQAKALIVFEGTKYGSQAPRVA 489
           +N+E EGEEL AD HILPAT+LGA A KAI  Y  S+K +  ALIVF+GT YG+ AP +A
Sbjct: 431 LNSEDEGEELLADAHILPATSLGASAAKAIRKYVGSAK-KPSALIVFQGTVYGNTAPVMA 490

Query: 490 AFSSRGPSLVGPDVIKPDVTAPGVNILAAWPPIVSPSELESDTRRVLFNIISGTSMSCPH 549
           AFSSRGP+  GPDVIKPDVTAPGV+ILAAWPP +SPS LESD R VLFNIISGTSMSCPH
Sbjct: 491 AFSSRGPNSAGPDVIKPDVTAPGVDILAAWPPNISPSMLESDNRSVLFNIISGTSMSCPH 550

Query: 550 VSGLAALLKSAHNDWSPAAIKSALMTTAYITDNKMSLISDVGQ-ANGEPATPFTFGSGHV 609
           VSGLA+LLKS H DWSPAAIKSALMTTAY  +NK + I+D+G  +  + ATPF FGSGHV
Sbjct: 551 VSGLASLLKSVHRDWSPAAIKSALMTTAYTLNNKGAPIADIGSTSTSKSATPFAFGSGHV 610

Query: 610 DPEKASDPGLIYDITPQDYINYLCSLKYNSTQIALVSRG-NFTCSSKRTVVKPGDLNYPS 669
           DPE A+DPGL+YDIT +DY+ YLCSL YNS+QIAL S G NFTC  K  V++PGDLNYPS
Sbjct: 611 DPENAADPGLVYDITAEDYLFYLCSLSYNSSQIALFSSGVNFTC-PKNAVLQPGDLNYPS 670

Query: 670 FSVFMKKKAKKVSITLKRTVTNVGISRSDYTVKINNPKGITVIVKPEKLSFGSLGEQLSY 729
           FSV   K A+ +S+T KRTV NVG   S Y V++  P G++V V+P  L F  +GE+LSY
Sbjct: 671 FSVLFSKDARNMSVTYKRTVKNVGKIPSTYAVQVKEPTGVSVTVEPRSLRFKKMGEKLSY 730

Query: 730 QVRFVSLGGKEALDTFSFGSLVWISGKYAVRSPIAVTW 766
           +V FV+LGG   L   SFG+L W+SGKY V SPIAVTW
Sbjct: 731 KVSFVALGG-PTLTNSSFGTLTWVSGKYRVGSPIAVTW 764

BLAST of Cucsa.234900 vs. TrEMBL
Match: K7KCA4_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_03G019000 PE=3 SV=1)

HSP 1 Score: 982.6 bits (2539), Expect = 2.7e-283
Identity = 490/771 (63.55%), Postives = 597/771 (77.43%), Query Frame = 1

Query: 1   MGFREVWVLLSIMLAVSSAVVDQQTYIIHMDATKMVTPIPEQ-----WYTDIIDSVNKLS 60
           M FR +  LL+ M+  S AV+++QTYIIHMD TK+   +  Q     W+  +ID +++ S
Sbjct: 1   MIFRTLLFLLAYMVTNSVAVMNKQTYIIHMDKTKIKATVHSQDKTKPWFKSVIDFISEAS 60

Query: 61  SLDDNEEEASNAAEILYVYKTALSGFAAKLTSKKLHSLSKIPGFLAATPNELLQLHTTHS 120
           S  + EE    A ++LYVY+T++ GFAA+L++K+L  L++I GFL+A P+ELL LHTT+S
Sbjct: 61  SSSEEEE----APQLLYVYETSMFGFAAQLSNKQLEYLNQIDGFLSAIPDELLTLHTTYS 120

Query: 121 PQFLGLQRDHGLWNSSNLASDIIIGLLDTGVWPEHISFQDESLSSVPLKWKGICQTGPRF 180
           P FLGLQ   GLW++SNLASD+IIG+LDTG+WPEHISFQD  LS VP +WKG C+ G  F
Sbjct: 121 PHFLGLQNGKGLWSASNLASDVIIGVLDTGIWPEHISFQDTGLSKVPSRWKGACEVGTNF 180

Query: 181 SSSNCNKKLIGASFYIKGYEAIVGRLNETGIFRSPRDSNGHGTHTASTAAGSIVNNASFF 240
           SSS CNKKL+GA  +++GYE   GR+NET  +RS RD+ GHGTHTASTAAG++V+NASFF
Sbjct: 181 SSSCCNKKLVGARVFLQGYEKSAGRINETLDYRSARDAQGHGTHTASTAAGNMVSNASFF 240

Query: 241 NQGMGVASGIRFTSRIVAYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGGSSSFYK 300
               G ASG+R+TSRI AYKVCW LGCAN+DILAA+D AVADGVDVLSLSLGG +  +Y 
Sbjct: 241 GLAGGSASGMRYTSRIAAYKVCWRLGCANSDILAAIDQAVADGVDVLSLSLGGIAKPYYN 300

Query: 301 DNIAIAAFGAIEKGVFVSCSAGNSGPSPSTVGNAAPWIMTVAASYTDRTFPTTVKLGNGQ 360
           D+IAIA+FGA +KGVFVSCSAGNSGPS ST GN APWIMTVAASYTDR+FPT VKLGNG+
Sbjct: 301 DSIAIASFGATQKGVFVSCSAGNSGPSSSTAGNVAPWIMTVAASYTDRSFPTQVKLGNGK 360

Query: 361 VFEGSSLYYGKSINELPLVYNNTAGDGQETNFCIAGSLDPSMVKGKIVVCERGQISRTEK 420
           VF+GSSLY GK  ++LPLVY N++   +   +C  GSLDP +VKGKIV CERG  SRT K
Sbjct: 361 VFKGSSLYKGKKTSQLPLVYRNSSRAQRTAQYCTKGSLDPKLVKGKIVACERGINSRTGK 420

Query: 421 GEQVKLAGGAGMILINTEFEGEELFADPHILPATTLGALAGKAILDYTASSKTQAKALIV 480
           GE+VK+AGGAGMIL+N+E +GEELFADPH+LPAT+LG+ A K I  Y   S     A I 
Sbjct: 421 GEEVKMAGGAGMILLNSENQGEELFADPHVLPATSLGSSASKTIRSYIFHSAKAPTASIS 480

Query: 481 FEGTKYGSQAPRVAAFSSRGPSLVGPDVIKPDVTAPGVNILAAWPPIVSPSELESDTRRV 540
           F GT YG  AP +AAFSSRGPS VGPDVIKPDVTAPGVNILAAWPP  SPS L+SD R V
Sbjct: 481 FLGTTYGDTAPVMAAFSSRGPSSVGPDVIKPDVTAPGVNILAAWPPTTSPSMLKSDKRSV 540

Query: 541 LFNIISGTSMSCPHVSGLAALLKSAHNDWSPAAIKSALMTTAYITDNKMSLISDVGQANG 600
           LFNI+SGTSMSCPHVSG+AAL+KS H DWSPAAIKSALMTTA  ++NK + ISD G  N 
Sbjct: 541 LFNIVSGTSMSCPHVSGIAALIKSVHKDWSPAAIKSALMTTASTSNNKGAPISDNGSNNS 600

Query: 601 EPATPFTFGSGHVDPEKASDPGLIYDITPQDYINYLCSLKYNSTQIALVSRGNFTCSSKR 660
             A PF FGSGHV+PE+ASDPGL+YDIT +DY+NYLCSLKY S+QIA++S+GNF C +K+
Sbjct: 601 AFADPFAFGSGHVNPERASDPGLVYDITTKDYLNYLCSLKYTSSQIAILSKGNFKC-AKK 660

Query: 661 TVVKPGDLNYPSFSVFMKKKAKKVSITLKRTVTNVGISRSDYTVKINNPKGITVIVKPEK 720
           + +  G LNYPSF+V     A+  S+T KR VTNVG   S Y VK+  PKG++V V+P  
Sbjct: 661 SALHAGGLNYPSFAVLFDTSARNASVTYKRVVTNVGNPSSSYAVKVEEPKGVSVTVEPRN 720

Query: 721 LSFGSLGEQLSYQVRFVSLGGKEALDTFSFGSLVWISGKYAVRSPIAVTWQ 767
           + F  +G++LSY+V FVS G      + SFGSL W+SGKYAVRSPIAVTWQ
Sbjct: 721 IGFRKIGDKLSYKVSFVSYGRTAVAGSSSFGSLTWVSGKYAVRSPIAVTWQ 766

BLAST of Cucsa.234900 vs. TrEMBL
Match: I1JDQ1_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_02G093400 PE=4 SV=2)

HSP 1 Score: 978.8 bits (2529), Expect = 3.8e-282
Identity = 482/770 (62.60%), Postives = 596/770 (77.40%), Query Frame = 1

Query: 3   FREVWVLLSIMLAVSSAVVDQQTYIIHMDATKMVTPIPEQ-----WYTDIIDSVNKLSSL 62
           FR + + L++M+  S A  DQQTYI+HMD TK+   I  Q     W+  IID +++ S  
Sbjct: 5   FRILILFLALMVTNSIAFADQQTYIVHMDQTKIKASIHTQDSTKPWFESIIDFISESSMQ 64

Query: 63  DDNEEEASNAAEILYVYKTALSGFAAKLTSKKLHSLSKIPGFLAATPNELLQLHTTHSPQ 122
           +++EE+ + A ++LY Y+T++ GFAA L+ K L  L+++ GFL+A P+EL  LHTT++P 
Sbjct: 65  EEDEEDDNLAPQLLYTYETSMFGFAAHLSKKHLKYLNQVDGFLSAIPDELSTLHTTYTPH 124

Query: 123 FLGLQRDHGLWNSSNLASDIIIGLLDTGVWPEHISFQDESLSSVPLKWKGICQTGPRFSS 182
           FLGL+    LW++SNLA+D+IIG+LD+G+WPEHISFQD  +S VP  WKG+C+ G +FSS
Sbjct: 125 FLGLRNGRSLWSASNLATDVIIGVLDSGIWPEHISFQDSGMSPVPSHWKGVCEKGTKFSS 184

Query: 183 SNCNKKLIGASFYIKGYEAIVGR-LNETGIFRSPRDSNGHGTHTASTAAGSIVNNASFFN 242
           SNCNKKL+GA  Y KGYE   G+ +NET  + SPRDS GHGTHTAST+AG++V NA+FF 
Sbjct: 185 SNCNKKLVGARAYYKGYEIFFGKKINETVDYLSPRDSQGHGTHTASTSAGNVVKNANFFG 244

Query: 243 QGMGVASGIRFTSRIVAYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGGSSSFYKD 302
           Q  G A G+R+TSRI  YKVCW  GC NAD+LAAMD AV+DGVDVLSLSLG     FY D
Sbjct: 245 QARGTACGMRYTSRIAVYKVCWSSGCTNADVLAAMDQAVSDGVDVLSLSLGSIPKPFYSD 304

Query: 303 NIAIAAFGAIEKGVFVSCSAGNSGPSPSTVGNAAPWIMTVAASYTDRTFPTTVKLGNGQV 362
           +IAIA++GAI+KGV V+CSAGNSGP PSTVGN APWIMTVAAS TDR+FPT VKLGNG+ 
Sbjct: 305 SIAIASYGAIKKGVLVACSAGNSGPFPSTVGNGAPWIMTVAASSTDRSFPTKVKLGNGKT 364

Query: 363 FEGSSLYYGKSINELPLVYNNTAGDGQETNFCIAGSLDPSMVKGKIVVCERGQISRTEKG 422
           F+GSSLY GK  N+LPLVY  +AG  +E  +CI GSLDP +V GKIV CERG   RTEKG
Sbjct: 365 FKGSSLYQGKKTNQLPLVYGKSAGAKKEAQYCIGGSLDPKLVHGKIVACERGINGRTEKG 424

Query: 423 EQVKLAGGAGMILINTEFEGEELFADPHILPATTLGALAGKAILDYTASSKTQAKALIVF 482
           E+VK+AGGAGMIL+N E++GEELFADPHILPAT+LGA A K I  Y+ S K +  A I F
Sbjct: 425 EEVKVAGGAGMILLNNEYQGEELFADPHILPATSLGASASKTIRSYSQSVK-KPTASISF 484

Query: 483 EGTKYGSQAPRVAAFSSRGPSLVGPDVIKPDVTAPGVNILAAWPPIVSPSELESDTRRVL 542
            GT++G  AP +AAFSSRGPSLVGPDVIKPDVTAPGVNILAAWP  +SPS L SD R+VL
Sbjct: 485 MGTRFGDPAPVMAAFSSRGPSLVGPDVIKPDVTAPGVNILAAWPTKISPSFLMSDKRKVL 544

Query: 543 FNIISGTSMSCPHVSGLAALLKSAHNDWSPAAIKSALMTTAYITDNKMSLISDVGQANGE 602
           FNI+SGTSMSCPHVSG+AALLKS H DWSPAAIKSALMTTAY  +NK + ISD+   N  
Sbjct: 545 FNILSGTSMSCPHVSGIAALLKSLHKDWSPAAIKSALMTTAYTLNNKGAPISDMASNNSP 604

Query: 603 PATPFTFGSGHVDPEKASDPGLIYDITPQDYINYLCSLKYNSTQIALVSRGNFTCSSKRT 662
            ATPF FGSGHV+P  ASDPGL+YDI+ +DY+NYLCS+ Y S+QIAL+SRG F C SK+ 
Sbjct: 605 LATPFAFGSGHVNPVSASDPGLVYDISTKDYLNYLCSINYTSSQIALLSRGKFVC-SKKA 664

Query: 663 VVKPGDLNYPSFSVFMKKKAKKVSITLKRTVTNVGISRSDYTVKINNPKGITVIVKPEKL 722
           V++ GDLNYPSF+V + K A  VS+T +R VTNVG  +S Y VK+  P G++V V+P KL
Sbjct: 665 VLQAGDLNYPSFAVLLGKSALNVSVTYRRVVTNVGKPQSAYAVKLEQPNGVSVTVEPRKL 724

Query: 723 SFGSLGEQLSYQVRFVSLGGKEALDTFSFGSLVWISGKYAVRSPIAVTWQ 767
            F  +G++LSY+V F+S+GG     T SFGSL+W+SG+Y VRSP+AVTWQ
Sbjct: 725 KFEKVGQKLSYKVTFLSIGGARVAGTSSFGSLIWVSGRYQVRSPMAVTWQ 772

BLAST of Cucsa.234900 vs. TrEMBL
Match: A0A0L9VRL6_PHAAN (Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan11g043200 PE=3 SV=1)

HSP 1 Score: 974.2 bits (2517), Expect = 9.5e-281
Identity = 481/777 (61.90%), Postives = 594/777 (76.45%), Query Frame = 1

Query: 1   MGFREVWVLLSIMLAVSSAVVDQQTYIIHMDATKMVTPIPEQ-----------WYTDIID 60
           M FR + + L+ M+  S AVV++QTYI+HMD TKM   +  Q           W+  +ID
Sbjct: 1   MNFRALLLFLAFMVTKSVAVVEKQTYIVHMDKTKMEASVHSQGLAKPWFKSKPWFKSVID 60

Query: 61  SVNKLSSLDDNEEEASNAAEILYVYKTALSGFAAKLTSKKLHSLSKIPGFLAATPNELLQ 120
            +++ S     EEE     ++LYVY+T+L GFAA+L++K+L  L+++ GF+AA P+ELL 
Sbjct: 61  FISEASF---EEEEGGGEPQLLYVYETSLFGFAAQLSNKQLEYLNQVDGFVAALPDELLT 120

Query: 121 LHTTHSPQFLGLQRDHGLWNSSNLASDIIIGLLDTGVWPEHISFQDESLSSVPLKWKGIC 180
           LHTT++P FLGLQ   GLW++SNLASD+IIG+LDTG+WPEHISFQD  LS VP +WKG C
Sbjct: 121 LHTTYTPHFLGLQEGEGLWSASNLASDVIIGVLDTGIWPEHISFQDTGLSKVPSRWKGAC 180

Query: 181 QTGPRFSSSNCNKKLIGASFYIKGYEAIVGRLNETGIFRSPRDSNGHGTHTASTAAGSIV 240
           + G  FS+S+CNKKL+GA  +++GYE   GR+NET  +RS RD+ GHG+HTASTAAG++V
Sbjct: 181 EAGTNFSASSCNKKLVGARVFLQGYEKFAGRINETMDYRSARDAQGHGSHTASTAAGNMV 240

Query: 241 NNASFFNQGMGVASGIRFTSRIVAYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGG 300
            NASFF    G ASG+R+TSRI AYKVCW LGCAN+DILAA+D AVADGVDVLSLSLGG 
Sbjct: 241 KNASFFGLASGSASGMRYTSRIAAYKVCWRLGCANSDILAAIDKAVADGVDVLSLSLGGS 300

Query: 301 SSSFYKDNIAIAAFGAIEKGVFVSCSAGNSGPSPSTVGNAAPWIMTVAASYTDRTFPTTV 360
           +  +Y D+IAIA+FGA + GVFVSCSAGNSGP  STVGN APWIMTVAASYTDR+FPT V
Sbjct: 301 ARPYYSDSIAIASFGATQNGVFVSCSAGNSGPFSSTVGNVAPWIMTVAASYTDRSFPTQV 360

Query: 361 KLGNGQVFEGSSLYYGKSINELPLVYNNTAGDGQETNFCIAGSLDPSMVKGKIVVCERGQ 420
           KLGNG++F+GSSLY GK  N+LPLVY N++ + +   +C  GSLDP  VKGKIV CERG 
Sbjct: 361 KLGNGKLFKGSSLYKGKKTNQLPLVYVNSSKEHRTAQYCTKGSLDPKFVKGKIVACERGI 420

Query: 421 ISRTEKGEQVKLAGGAGMILINTEFEGEELFADPHILPATTLGALAGKAILDYTASSKTQ 480
            SRT KGE+VK+AGGAGMIL+N+E +GEELFAD H+LP T+LGA A K I  Y  S+K  
Sbjct: 421 NSRTAKGEEVKMAGGAGMILLNSENQGEELFADHHVLPGTSLGASASKIIRSYIHSAKAP 480

Query: 481 AKALIVFEGTKYGSQAPRVAAFSSRGPSLVGPDVIKPDVTAPGVNILAAWPPIVSPSELE 540
             A I F+GT YG  AP +AAFSSRGPS VG DVIKPDVTAPGVNILAAWPPI SPS L+
Sbjct: 481 T-ASISFQGTAYGDPAPAMAAFSSRGPSAVGGDVIKPDVTAPGVNILAAWPPITSPSMLK 540

Query: 541 SDTRRVLFNIISGTSMSCPHVSGLAALLKSAHNDWSPAAIKSALMTTAYITDNKMSLISD 600
           SD    +FNI+SGTSMSCPHVSG+AAL+KSAHN WSPAAIKSALMTTA +++NK + I+D
Sbjct: 541 SDKTSAVFNIVSGTSMSCPHVSGIAALIKSAHNGWSPAAIKSALMTTASVSNNKGAAIAD 600

Query: 601 VGQANGEPATPFTFGSGHVDPEKASDPGLIYDITPQDYINYLCSLKYNSTQIALVSRGNF 660
            G      A PF FGSGHV+PE+ASDPGL+YDIT +DY+NYLCSLKY  +QIAL+S+GNF
Sbjct: 601 YGSKTSALADPFAFGSGHVNPERASDPGLVYDITTKDYLNYLCSLKYTPSQIALLSKGNF 660

Query: 661 TCSSKRTVVKPGDLNYPSFSVFMKKKAKKVSITLKRTVTNVGISRSDYTVKINNPKGITV 720
           TC  K++ ++ GDLNYPSF+V    +A K S+T KR VTNVG  +  Y VK+  P G++V
Sbjct: 661 TC-PKKSALQAGDLNYPSFAVLFGTRALKASVTFKRVVTNVGNPKISYAVKVEEPNGVSV 720

Query: 721 IVKPEKLSFGSLGEQLSYQVRFVSLGGKEALDTFSFGSLVWISGKYAVRSPIAVTWQ 767
            V+P  +SF   G+++SY+V FVS G        SFGSL W+SGKYAVRSPIAVTW+
Sbjct: 721 RVEPRNISFRKTGQKMSYKVSFVSNGNTTVTGNSSFGSLTWVSGKYAVRSPIAVTWK 772

BLAST of Cucsa.234900 vs. TAIR10
Match: AT1G01900.1 (AT1G01900.1 subtilase family protein)

HSP 1 Score: 841.3 bits (2172), Expect = 4.8e-244
Identity = 426/767 (55.54%), Postives = 542/767 (70.66%), Query Frame = 1

Query: 1   MGFREVWVLLSIMLAVSSAVVDQQTYIIHMDATKMVTPIPEQWYTDIIDSVNKLSSLDDN 60
           M FR   V   ++   S+    +QTY+IH      VT   +   T + +S+   +  DD+
Sbjct: 19  MFFRSFIVFFFLIFFASNVSSRKQTYVIHT-----VTTSTKHIVTSLFNSLQTENINDDD 78

Query: 61  EEEASNAAEILYVYKTALSGFAAKLTSKKLHSLSKIPGFLAATPNELLQLHTTHSPQFLG 120
                +  EI Y+Y+ A+SGF+A LT  +L ++    GF++A P+ELL LHTT+S +FLG
Sbjct: 79  ----FSLPEIHYIYENAMSGFSATLTDDQLDTVKNTKGFISAYPDELLSLHTTYSHEFLG 138

Query: 121 LQRDHGLWNSSNLASDIIIGLLDTGVWPEHISFQDESLSSVPLKWKGICQTGPRFSSSNC 180
           L+   GLWN ++L+SD+IIGL+DTG+ PEH+SF+D  ++ VP +W+G C  G  FSSS C
Sbjct: 139 LEFGIGLWNETSLSSDVIIGLVDTGISPEHVSFRDTHMTPVPSRWRGSCDEGTNFSSSEC 198

Query: 181 NKKLIGASFYIKGYEAIVGRLNETGIFRSPRDSNGHGTHTASTAAGSIVNNASFFNQGMG 240
           NKK+IGAS + KGYE+IVG++NET  FRS RD+ GHGTHTASTAAG IV  A++F Q  G
Sbjct: 199 NKKIIGASAFYKGYESIVGKINETTDFRSTRDAQGHGTHTASTAAGDIVPKANYFGQAKG 258

Query: 241 VASGIRFTSRIVAYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGGSSSFYKDNIAI 300
           +ASG+RFTSRI AYK CW LGCA+ D++AA+D A+ DGVDV+SLSLGG S  FY D IAI
Sbjct: 259 LASGMRFTSRIAAYKACWALGCASTDVIAAIDRAILDGVDVISLSLGGSSRPFYVDPIAI 318

Query: 301 AAFGAIEKGVFVSCSAGNSGPSPSTVGNAAPWIMTVAASYTDRTFPTTVKLGNGQVFEGS 360
           A FGA++K +FVSCSAGNSGP+ STV N APW+MTVAASYTDRTFP  V++GN +   GS
Sbjct: 319 AGFGAMQKNIFVSCSAGNSGPTASTVSNGAPWLMTVAASYTDRTFPAIVRIGNRKSLVGS 378

Query: 361 SLYYGKSINELPLVYNNTAGDGQETNFCIAGSLDPSMVKGKIVVCERGQISRTEKGEQVK 420
           SLY GKS+  LPL +N TAG+     FCI  SL   +V+GKIV+C RG   RT KGE+VK
Sbjct: 379 SLYKGKSLKNLPLAFNRTAGEESGAVFCIRDSLKRELVEGKIVICLRGASGRTAKGEEVK 438

Query: 421 LAGGAGMILINTEFEGEELFADPHILPATTLGALAGKAILDYTASSKTQAKALIVFEGTK 480
            +GGA M+L++TE EGEEL ADPH+LPA +LG   GK +L+Y A +   A A + F GT 
Sbjct: 439 RSGGAAMLLVSTEAEGEELLADPHVLPAVSLGFSDGKTLLNYLAGA-ANATASVRFRGTA 498

Query: 481 YGSQAPRVAAFSSRGPSLVGPDVIKPDVTAPGVNILAAWPPIVSPSELESDTRRVLFNII 540
           YG+ AP VAAFSSRGPS+ GP++ KPD+ APG+NILA W P  SPS L SD RRV FNII
Sbjct: 499 YGATAPMVAAFSSRGPSVAGPEIAKPDIAAPGLNILAGWSPFSSPSLLRSDPRRVQFNII 558

Query: 541 SGTSMSCPHVSGLAALLKSAHNDWSPAAIKSALMTTAYITDNKMSLISDVGQANGE-PAT 600
           SGTSM+CPH+SG+AAL+KS H DWSPA IKSA+MTTA ITDN+   I D G A  E  AT
Sbjct: 559 SGTSMACPHISGIAALIKSVHGDWSPAMIKSAIMTTARITDNRNRPIGDRGAAGAESAAT 618

Query: 601 PFTFGSGHVDPEKASDPGLIYDITPQDYINYLCSLKYNSTQIALVSRGNFTCSSKRTVVK 660
            F FG+G+VDP +A DPGL+YD +  DY+NYLCSL Y S +I L S  N+TC+S   V+ 
Sbjct: 619 AFAFGAGNVDPTRAVDPGLVYDTSTVDYLNYLCSLNYTSERILLFSGTNYTCASNAVVLS 678

Query: 661 PGDLNYPSFSVFMKKKAKKVSITLKRTVTNVGISRSDYTVKINNPKGITVIVKPEKLSFG 720
           PGDLNYPSF+V +   A   ++  KRTVTNVG    +Y V +  PKG+ V V+P+ L F 
Sbjct: 679 PGDLNYPSFAVNLVNGANLKTVRYKRTVTNVGSPTCEYMVHVEEPKGVKVRVEPKVLKFQ 738

Query: 721 SLGEQLSYQVRFVSLGGKEALDTFSFGSLVWISGKYAVRSPIAVTWQ 767
              E+LSY V + +   + +  + SFG LVWI  KY VRSPIAVTW+
Sbjct: 739 KARERLSYTVTYDAEASRNS-SSSSFGVLVWICDKYNVRSPIAVTWE 774

BLAST of Cucsa.234900 vs. TAIR10
Match: AT5G51750.1 (AT5G51750.1 subtilase 1.3)

HSP 1 Score: 705.7 bits (1820), Expect = 3.2e-203
Identity = 369/769 (47.98%), Postives = 504/769 (65.54%), Query Frame = 1

Query: 9   LLSIMLAVSSAVVDQQTYIIHMDATKMVTPIPE--QWYTDIIDSVNKLSSLDDNEEEASN 68
           L+ +    ++ +  ++TY+IHMD + M  P     QWY+  I+SV +  S    +EE  N
Sbjct: 20  LIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKS----QEEEGN 79

Query: 69  AAEILYVYKTALSGFAAKLTSKKLHSLSKIPGFLAATPNELLQLHTTHSPQFLGLQRDHG 128
              ILY Y+TA  G AA+LT ++   L +  G +A  P    +LHTT SP FLGL+R   
Sbjct: 80  NNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLERQES 139

Query: 129 --LWNSSNLASDIIIGLLDTGVWPEHISFQDESLSSVPLKWKGICQTGPRFSSSNCNKKL 188
             +W       D+++G+LDTG+WPE  SF D  +S VP  W+G C+TG RF   NCN+K+
Sbjct: 140 ERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNCNRKI 199

Query: 189 IGASFYIKGYEAIVGRLNETGIFRSPRDSNGHGTHTASTAAGSIVNNASFFNQGMGVASG 248
           +GA  + +GYEA  G+++E   ++SPRD +GHGTHTA+T AGS V  A+ F    G A G
Sbjct: 200 VGARVFYRGYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYGTARG 259

Query: 249 IRFTSRIVAYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGGSSSFYKDNIAIAAFG 308
           +   +R+ AYKVCW  GC ++DIL+A+D AVADGV VLS+SLGGG S++ +D+++IA FG
Sbjct: 260 MAQKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRDSLSIATFG 319

Query: 309 AIEKGVFVSCSAGNSGPSPSTVGNAAPWIMTVAASYTDRTFPTTVKLGNGQVFEGSSLYY 368
           A+E GVFVSCSAGN GP P ++ N +PWI TV AS  DR FP TVK+G  + F+G SLY 
Sbjct: 320 AMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFKGVSLYK 379

Query: 369 GKSI----NELPLVY-NNTAGDGQETNFCIAGSLDPSMVKGKIVVCERGQISRTEKGEQV 428
           G+++     + PLVY    A     T+FC+ G+LD   V GKIV+C+RG   R +KG+ V
Sbjct: 380 GRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRVQKGQVV 439

Query: 429 KLAGGAGMILINTEFEGEELFADPHILPATTLGALAGKAILDYTASSKTQAKALIVFEGT 488
           K AGG GM+L NT   GEEL AD H+LPA  +G   GK I  Y  +SK +A A +   GT
Sbjct: 440 KRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSK-KATASLEILGT 499

Query: 489 KYG-SQAPRVAAFSSRGPSLVGPDVIKPDVTAPGVNILAAWPPIVSPSELESDTRRVLFN 548
           + G   +P VAAFSSRGP+ +  +++KPD+ APGVNILAAW   ++PS L SD RRV FN
Sbjct: 500 RIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPRRVKFN 559

Query: 549 IISGTSMSCPHVSGLAALLKSAHNDWSPAAIKSALMTTAYITDNKMSLISDVGQANGEPA 608
           I+SGTSMSCPHVSG+AAL+KS H DWSPAAIKSALMTTAY+ DN    ++D   A   P+
Sbjct: 560 ILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGA--APS 619

Query: 609 TPFTFGSGHVDPEKASDPGLIYDITPQDYINYLCSLKYNSTQIALVSR-GNFTCSSKRTV 668
           +P+  G+GH+DP +A+DPGL+YDI PQ+Y  +LC+   + +Q+ + ++  N TC  K T+
Sbjct: 620 SPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTC--KHTL 679

Query: 669 VK-PGDLNYPSFSVFMKKKAKKVSITLKRTVTNVGISRSDYTVKINNPKGITVIVKPEKL 728
            K PG+LNYP+ S    +     ++TL+RTVTNVG   S Y V ++  KG +V V+P+ L
Sbjct: 680 AKNPGNLNYPAISALFPENTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGASVTVQPKTL 739

Query: 729 SFGSLGEQLSYQVRFVSLGGKEALDTFSFGSLVWISGKYAVRSPIAVTW 766
           +F S  ++LSY V F +   +  +    FG LVW S  + VRSP+ +TW
Sbjct: 740 NFTSKHQKLSYTVTFRT---RFRMKRPEFGGLVWKSTTHKVRSPVIITW 776

BLAST of Cucsa.234900 vs. TAIR10
Match: AT3G14240.1 (AT3G14240.1 Subtilase family protein)

HSP 1 Score: 699.1 bits (1803), Expect = 3.0e-201
Identity = 382/768 (49.74%), Postives = 493/768 (64.19%), Query Frame = 1

Query: 17  SSAVVDQQTYIIHMD--ATKMVTPIPEQWYTDIIDSVNKLSSLDDNEEEASNAAEILYVY 76
           S++  +  TYI+H+D  A   + P    WYT  + S+             S+   I++ Y
Sbjct: 19  SASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLT------------SSPPSIIHTY 78

Query: 77  KTALSGFAAKLTSKKLHSLSKIPGFLAATPNELLQLHTTHSPQFLGLQRDH--GLWNSSN 136
            T   GF+A+LTS+    L   P  ++  P ++  LHTT SP+FLGL+     GL   S+
Sbjct: 79  DTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEESD 138

Query: 137 LASDIIIGLLDTGVWPEHISFQDESLSSVPLKWKGICQTGPRFSSSNCNKKLIGASFYIK 196
             SD++IG++DTGVWPE  SF D  L  VP+KWKG C     F  S CN+KL+GA F+  
Sbjct: 139 FGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCG 198

Query: 197 GYEAIVGRLNETGIFRSPRDSNGHGTHTASTAAGSIVNNASFFNQGMGVASGIRFTSRIV 256
           GYEA  G++NET  FRSPRDS+GHGTHTAS +AG  V  AS      GVA+G+   +R+ 
Sbjct: 199 GYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLA 258

Query: 257 AYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGGSSSFYKDNIAIAAFGAIEKGVFV 316
           AYKVCW  GC ++DILAA D+AVADGVDV+SLS+GG    +Y D IAI AFGAI++G+FV
Sbjct: 259 AYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFV 318

Query: 317 SCSAGNSGPSPSTVGNAAPWIMTVAASYTDRTFPTTVKLGNGQVFEGSSLYYGKSINE-- 376
           S SAGN GP   TV N APW+ TV A   DR FP  VKLGNG++  G S+Y G  ++   
Sbjct: 319 SASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDPGR 378

Query: 377 -LPLVYNNT--AGDGQETNFCIAGSLDPSMVKGKIVVCERGQISRTEKGEQVKLAGGAGM 436
             PLVY  +   GDG  ++ C+ GSLDP++VKGKIV+C+RG  SR  KGE V+  GG GM
Sbjct: 379 MYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKNGGLGM 438

Query: 437 ILINTEFEGEELFADPHILPATTLGALAGKAILDY-TASSKTQAK----ALIVFEGTKYG 496
           I+ N  F+GE L AD H+LPAT++GA  G  I  Y + SSK+++     A IVF+GT+ G
Sbjct: 439 IIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLG 498

Query: 497 SQ-APRVAAFSSRGPSLVGPDVIKPDVTAPGVNILAAWPPIVSPSELESDTRRVLFNIIS 556
            + AP VA+FS+RGP+   P+++KPDV APG+NILAAWP  + PS + SD RR  FNI+S
Sbjct: 499 IRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILS 558

Query: 557 GTSMSCPHVSGLAALLKSAHNDWSPAAIKSALMTTAYITDNKMSLISDVGQANGEPATPF 616
           GTSM+CPHVSGLAALLK+AH DWSPAAI+SAL+TTAY  DN    + D  ++ G  ++  
Sbjct: 559 GTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMD--ESTGNTSSVM 618

Query: 617 TFGSGHVDPEKASDPGLIYDITPQDYINYLCSLKYNSTQIALVSRGNFTCSSKRTVVKPG 676
            +GSGHV P KA DPGL+YDIT  DYIN+LC+  Y  T I  ++R    C   R     G
Sbjct: 619 DYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAGHVG 678

Query: 677 DLNYPSFS-VFMKKKAKKVSITLKRTVTNVGISRSDYTVKINNPKGITVIVKPEKLSFGS 736
           +LNYPSFS VF +    K+S    RTVTNVG S S Y +KI  P+G TV V+PEKLSF  
Sbjct: 679 NLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFRR 738

Query: 737 LGEQLSYQVRFVSLGGK--EALDTFSFGSLVWISGKYAVRSPIAVTWQ 767
           +G++LS+ VR  +   K          G +VW  GK  V SP+ VT Q
Sbjct: 739 VGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVTLQ 772

BLAST of Cucsa.234900 vs. TAIR10
Match: AT5G67360.1 (AT5G67360.1 Subtilase family protein)

HSP 1 Score: 688.3 bits (1775), Expect = 5.2e-198
Identity = 362/757 (47.82%), Postives = 492/757 (64.99%), Query Frame = 1

Query: 16  VSSAVVDQQTYIIHMDATKMVTP--IPEQWYTDIIDSVNKLSSLDDNEEEASNAAEILYV 75
           VSS+  DQ TYI+HM  ++M +   +   WY   + S+             S++AE+LY 
Sbjct: 22  VSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSI-------------SDSAELLYT 81

Query: 76  YKTALSGFAAKLTSKKLHSLSKIPGFLAATPNELLQLHTTHSPQFLGL-QRDHGLWNSSN 135
           Y+ A+ GF+ +LT ++  SL   PG ++  P    +LHTT +P FLGL +    L+  + 
Sbjct: 82  YENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEAG 141

Query: 136 LASDIIIGLLDTGVWPEHISFQDESLSSVPLKWKGICQTGPRFSSSNCNKKLIGASFYIK 195
             SD+++G+LDTGVWPE  S+ DE    +P  WKG C+ G  F++S CN+KLIGA F+ +
Sbjct: 142 SYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFAR 201

Query: 196 GYEAIVGRLNETGIFRSPRDSNGHGTHTASTAAGSIVNNASFFNQGMGVASGIRFTSRIV 255
           GYE+ +G ++E+   RSPRD +GHGTHT+STAAGS+V  AS      G A G+   +R+ 
Sbjct: 202 GYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVA 261

Query: 256 AYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGGSSSFYKDNIAIAAFGAIEKGVFV 315
            YKVCW  GC ++DILAA+D A+AD V+VLS+SLGGG S +Y+D +AI AF A+E+G+ V
Sbjct: 262 VYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILV 321

Query: 316 SCSAGNSGPSPSTVGNAAPWIMTVAASYTDRTFPTTVKLGNGQVFEGSSLYYGKSINE-- 375
           SCSAGN+GPS S++ N APWI TV A   DR FP    LGNG+ F G SL+ G+++ +  
Sbjct: 322 SCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDKL 381

Query: 376 LPLVYNNTAGDGQETNFCIAGSLDPSMVKGKIVVCERGQISRTEKGEQVKLAGGAGMILI 435
           LP +Y   A +    N C+ G+L P  VKGKIV+C+RG  +R +KG+ VK AGG GMIL 
Sbjct: 382 LPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILA 441

Query: 436 NTEFEGEELFADPHILPATTLGALAGKAILDYTASSKTQAKALIVFEGTKYG-SQAPRVA 495
           NT   GEEL AD H+LPATT+G  AG  I  Y  +      ++ +  GT  G   +P VA
Sbjct: 442 NTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISIL-GTVVGVKPSPVVA 501

Query: 496 AFSSRGPSLVGPDVIKPDVTAPGVNILAAWPPIVSPSELESDTRRVLFNIISGTSMSCPH 555
           AFSSRGP+ + P+++KPD+ APGVNILAAW     P+ L SD+RRV FNIISGTSMSCPH
Sbjct: 502 AFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPH 561

Query: 556 VSGLAALLKSAHNDWSPAAIKSALMTTAYITDNKMSLISDVGQANGEPATPFTFGSGHVD 615
           VSGLAALLKS H +WSPAAI+SALMTTAY T      + D+  A G+P+TPF  G+GHV 
Sbjct: 562 VSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDI--ATGKPSTPFDHGAGHVS 621

Query: 616 PEKASDPGLIYDITPQDYINYLCSLKYNSTQIALVSRGNFTCSSKRTVVKPGDLNYPSFS 675
           P  A++PGLIYD+T +DY+ +LC+L Y S QI  VSR N+TC   ++     DLNYPSF+
Sbjct: 622 PTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKS-YSVADLNYPSFA 681

Query: 676 VFMKKKAKKVSITLKRTVTNVGISRSDYTVKI-NNPKGITVIVKPEKLSFGSLGEQLSYQ 735
           V +       +    RTVT+VG     Y+VK+ +   G+ + V+P  L+F    E+ SY 
Sbjct: 682 VNVDGVG---AYKYTRTVTSVG-GAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYT 741

Query: 736 VRFVSLGGKEALDTFSFGSLVWISGKYAVRSPIAVTW 766
           V F ++   +   + SFGS+ W  GK+ V SP+A++W
Sbjct: 742 VTF-TVDSSKPSGSNSFGSIEWSDGKHVVGSPVAISW 756

BLAST of Cucsa.234900 vs. TAIR10
Match: AT2G05920.1 (AT2G05920.1 Subtilase family protein)

HSP 1 Score: 671.0 bits (1730), Expect = 8.7e-193
Identity = 353/707 (49.93%), Postives = 459/707 (64.92%), Query Frame = 1

Query: 63  EASNAAEILYVYKTALSGFAAKLTSKKLHSL-SKIPGFLAATPNELLQLHTTHSPQFLGL 122
           + ++ + +LY Y T+  GF+A L S +  SL S     L    + L  LHTT +P+FLGL
Sbjct: 53  QLNSESSLLYTYTTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGL 112

Query: 123 QRDHGLWNSSNLASDIIIGLLDTGVWPEHISFQDESLSSVPLKWKGICQTGPRFSSSNCN 182
             + G+ +  + ++ +IIG+LDTGVWPE  SF D  +  +P KWKG C++G  F S  CN
Sbjct: 113 NSEFGVHDLGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCN 172

Query: 183 KKLIGASFYIKGYE-AIVGRLNETGIFRSPRDSNGHGTHTASTAAGSIVNNASFFNQGMG 242
           KKLIGA  + KG++ A  G  +      SPRD +GHGTHT++TAAGS V NASF     G
Sbjct: 173 KKLIGARSFSKGFQMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAG 232

Query: 243 VASGIRFTSRIVAYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGGSSSFYKDNIAI 302
            A G+   +R+  YKVCW  GC  +DILAAMD A+ DGVDVLSLSLGGGS+ +Y+D IAI
Sbjct: 233 TARGMATRARVATYKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAI 292

Query: 303 AAFGAIEKGVFVSCSAGNSGPSPSTVGNAAPWIMTVAASYTDRTFPTTVKLGNGQVFEGS 362
            AF A+E+GVFVSCSAGNSGP+ ++V N APW+MTV A   DR FP    LGNG+   G 
Sbjct: 293 GAFSAMERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGV 352

Query: 363 SLYYGKSINELPLVYNNTAGDGQETNFCIAGSLDPSMVKGKIVVCERGQISRTEKGEQVK 422
           SLY G  +   PL      G+   +N C+ GSLD S+V+GKIVVC+RG  +R EKG  V+
Sbjct: 353 SLYSGVGMGTKPLELVYNKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVR 412

Query: 423 LAGGAGMILINTEFEGEELFADPHILPATTLGALAGKAILDYTASSKTQAKALIVFEGTK 482
            AGG GMI+ NT   GEEL AD H+LPA  +G   G  + +Y   S ++  AL+VF+GT 
Sbjct: 413 DAGGLGMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREY-VKSDSKPTALLVFKGTV 472

Query: 483 YG-SQAPRVAAFSSRGPSLVGPDVIKPDVTAPGVNILAAWPPIVSPSELESDTRRVLFNI 542
                +P VAAFSSRGP+ V P+++KPDV  PGVNILA W   + P+ L+ D+RR  FNI
Sbjct: 473 LDVKPSPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNI 532

Query: 543 ISGTSMSCPHVSGLAALLKSAHNDWSPAAIKSALMTTAYITDNKMSLISDVGQANGEPAT 602
           +SGTSMSCPH+SGLA LLK+AH +WSP+AIKSALMTTAY+ DN  + + D   A+   + 
Sbjct: 533 MSGTSMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDA--ADNSLSN 592

Query: 603 PFTFGSGHVDPEKASDPGLIYDITPQDYINYLCSLKYNSTQI-ALVSRGNFTCSSKRTVV 662
           P+  GSGHVDP+KA  PGL+YDI+ ++YI +LCSL Y    I A+V R +  CS K +  
Sbjct: 593 PYAHGSGHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFS-- 652

Query: 663 KPGDLNYPSFSVFMKKKAKKVSITLKRTVTNVGISRSDYTVKINNPKGITVIVKPEKLSF 722
            PG LNYPSFSV       K  +   R VTNVG + S Y V +N    + + VKP KLSF
Sbjct: 653 DPGQLNYPSFSVLF---GGKRVVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSF 712

Query: 723 GSLGEQLSYQVRFVSLGGKEALDTFSFGSLVWISGKYAVRSPIAVTW 766
            S+GE+  Y V FVS  G    +   FGS+ W + ++ VRSP+A +W
Sbjct: 713 KSVGEKKRYTVTFVSKKGVSMTNKAEFGSITWSNPQHEVRSPVAFSW 751

BLAST of Cucsa.234900 vs. NCBI nr
Match: gi|449445359|ref|XP_004140440.1| (PREDICTED: subtilisin-like protease SBT1.1 [Cucumis sativus])

HSP 1 Score: 1520.8 bits (3936), Expect = 0.0e+00
Identity = 766/766 (100.00%), Postives = 766/766 (100.00%), Query Frame = 1

Query: 1   MGFREVWVLLSIMLAVSSAVVDQQTYIIHMDATKMVTPIPEQWYTDIIDSVNKLSSLDDN 60
           MGFREVWVLLSIMLAVSSAVVDQQTYIIHMDATKMVTPIPEQWYTDIIDSVNKLSSLDDN
Sbjct: 1   MGFREVWVLLSIMLAVSSAVVDQQTYIIHMDATKMVTPIPEQWYTDIIDSVNKLSSLDDN 60

Query: 61  EEEASNAAEILYVYKTALSGFAAKLTSKKLHSLSKIPGFLAATPNELLQLHTTHSPQFLG 120
           EEEASNAAEILYVYKTALSGFAAKLTSKKLHSLSKIPGFLAATPNELLQLHTTHSPQFLG
Sbjct: 61  EEEASNAAEILYVYKTALSGFAAKLTSKKLHSLSKIPGFLAATPNELLQLHTTHSPQFLG 120

Query: 121 LQRDHGLWNSSNLASDIIIGLLDTGVWPEHISFQDESLSSVPLKWKGICQTGPRFSSSNC 180
           LQRDHGLWNSSNLASDIIIGLLDTGVWPEHISFQDESLSSVPLKWKGICQTGPRFSSSNC
Sbjct: 121 LQRDHGLWNSSNLASDIIIGLLDTGVWPEHISFQDESLSSVPLKWKGICQTGPRFSSSNC 180

Query: 181 NKKLIGASFYIKGYEAIVGRLNETGIFRSPRDSNGHGTHTASTAAGSIVNNASFFNQGMG 240
           NKKLIGASFYIKGYEAIVGRLNETGIFRSPRDSNGHGTHTASTAAGSIVNNASFFNQGMG
Sbjct: 181 NKKLIGASFYIKGYEAIVGRLNETGIFRSPRDSNGHGTHTASTAAGSIVNNASFFNQGMG 240

Query: 241 VASGIRFTSRIVAYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGGSSSFYKDNIAI 300
           VASGIRFTSRIVAYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGGSSSFYKDNIAI
Sbjct: 241 VASGIRFTSRIVAYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGGSSSFYKDNIAI 300

Query: 301 AAFGAIEKGVFVSCSAGNSGPSPSTVGNAAPWIMTVAASYTDRTFPTTVKLGNGQVFEGS 360
           AAFGAIEKGVFVSCSAGNSGPSPSTVGNAAPWIMTVAASYTDRTFPTTVKLGNGQVFEGS
Sbjct: 301 AAFGAIEKGVFVSCSAGNSGPSPSTVGNAAPWIMTVAASYTDRTFPTTVKLGNGQVFEGS 360

Query: 361 SLYYGKSINELPLVYNNTAGDGQETNFCIAGSLDPSMVKGKIVVCERGQISRTEKGEQVK 420
           SLYYGKSINELPLVYNNTAGDGQETNFCIAGSLDPSMVKGKIVVCERGQISRTEKGEQVK
Sbjct: 361 SLYYGKSINELPLVYNNTAGDGQETNFCIAGSLDPSMVKGKIVVCERGQISRTEKGEQVK 420

Query: 421 LAGGAGMILINTEFEGEELFADPHILPATTLGALAGKAILDYTASSKTQAKALIVFEGTK 480
           LAGGAGMILINTEFEGEELFADPHILPATTLGALAGKAILDYTASSKTQAKALIVFEGTK
Sbjct: 421 LAGGAGMILINTEFEGEELFADPHILPATTLGALAGKAILDYTASSKTQAKALIVFEGTK 480

Query: 481 YGSQAPRVAAFSSRGPSLVGPDVIKPDVTAPGVNILAAWPPIVSPSELESDTRRVLFNII 540
           YGSQAPRVAAFSSRGPSLVGPDVIKPDVTAPGVNILAAWPPIVSPSELESDTRRVLFNII
Sbjct: 481 YGSQAPRVAAFSSRGPSLVGPDVIKPDVTAPGVNILAAWPPIVSPSELESDTRRVLFNII 540

Query: 541 SGTSMSCPHVSGLAALLKSAHNDWSPAAIKSALMTTAYITDNKMSLISDVGQANGEPATP 600
           SGTSMSCPHVSGLAALLKSAHNDWSPAAIKSALMTTAYITDNKMSLISDVGQANGEPATP
Sbjct: 541 SGTSMSCPHVSGLAALLKSAHNDWSPAAIKSALMTTAYITDNKMSLISDVGQANGEPATP 600

Query: 601 FTFGSGHVDPEKASDPGLIYDITPQDYINYLCSLKYNSTQIALVSRGNFTCSSKRTVVKP 660
           FTFGSGHVDPEKASDPGLIYDITPQDYINYLCSLKYNSTQIALVSRGNFTCSSKRTVVKP
Sbjct: 601 FTFGSGHVDPEKASDPGLIYDITPQDYINYLCSLKYNSTQIALVSRGNFTCSSKRTVVKP 660

Query: 661 GDLNYPSFSVFMKKKAKKVSITLKRTVTNVGISRSDYTVKINNPKGITVIVKPEKLSFGS 720
           GDLNYPSFSVFMKKKAKKVSITLKRTVTNVGISRSDYTVKINNPKGITVIVKPEKLSFGS
Sbjct: 661 GDLNYPSFSVFMKKKAKKVSITLKRTVTNVGISRSDYTVKINNPKGITVIVKPEKLSFGS 720

Query: 721 LGEQLSYQVRFVSLGGKEALDTFSFGSLVWISGKYAVRSPIAVTWQ 767
           LGEQLSYQVRFVSLGGKEALDTFSFGSLVWISGKYAVRSPIAVTWQ
Sbjct: 721 LGEQLSYQVRFVSLGGKEALDTFSFGSLVWISGKYAVRSPIAVTWQ 766

BLAST of Cucsa.234900 vs. NCBI nr
Match: gi|659109540|ref|XP_008454762.1| (PREDICTED: subtilisin-like protease [Cucumis melo])

HSP 1 Score: 1449.9 bits (3752), Expect = 0.0e+00
Identity = 728/766 (95.04%), Postives = 747/766 (97.52%), Query Frame = 1

Query: 1   MGFREVWVLLSIMLAVSSAVVDQQTYIIHMDATKMVTPIPEQWYTDIIDSVNKLSSLDDN 60
           MGFREVWV LSIMLA+SSAVVDQQTYIIHMD TKMVTP PEQWYTDIIDSVN+LSSLDDN
Sbjct: 1   MGFREVWV-LSIMLAISSAVVDQQTYIIHMDTTKMVTPNPEQWYTDIIDSVNELSSLDDN 60

Query: 61  EEEASNAAEILYVYKTALSGFAAKLTSKKLHSLSKIPGFLAATPNELLQLHTTHSPQFLG 120
            EEASNAAEILYVYKTALSGFAAKLTSKKLHSLSKIPGFLAATPNELLQLHTTHSPQFLG
Sbjct: 61  -EEASNAAEILYVYKTALSGFAAKLTSKKLHSLSKIPGFLAATPNELLQLHTTHSPQFLG 120

Query: 121 LQRDHGLWNSSNLASDIIIGLLDTGVWPEHISFQDESLSSVPLKWKGICQTGPRFSSSNC 180
           LQRDHGLWN SNLASDIIIGLLDTG+WPEHISFQD+ LSSVPLKWKGICQTGPRFSSSNC
Sbjct: 121 LQRDHGLWNFSNLASDIIIGLLDTGIWPEHISFQDKGLSSVPLKWKGICQTGPRFSSSNC 180

Query: 181 NKKLIGASFYIKGYEAIVGRLNETGIFRSPRDSNGHGTHTASTAAGSIVNNASFFNQGMG 240
           NKKLIGAS YIKGYEAIVGRLNETG FRSPRDS+GHGTHTASTAAGSIV+NASF+NQGMG
Sbjct: 181 NKKLIGASAYIKGYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGSIVDNASFYNQGMG 240

Query: 241 VASGIRFTSRIVAYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGGSSSFYKDNIAI 300
           VASG+RFTSRIVAYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGGSSSFYKDNIAI
Sbjct: 241 VASGMRFTSRIVAYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGGSSSFYKDNIAI 300

Query: 301 AAFGAIEKGVFVSCSAGNSGPSPSTVGNAAPWIMTVAASYTDRTFPTTVKLGNGQVFEGS 360
           AAFGAI+KGVFVSCSAGNSGPSPSTVGNAAPWIMTVAASYTDRTFPTTVKLGNGQVFEGS
Sbjct: 301 AAFGAIQKGVFVSCSAGNSGPSPSTVGNAAPWIMTVAASYTDRTFPTTVKLGNGQVFEGS 360

Query: 361 SLYYGKSINELPLVYNNTAGDGQETNFCIAGSLDPSMVKGKIVVCERGQISRTEKGEQVK 420
           SLYYGKSINELPLVYNNTAGDGQETN CIAGSLDPSMVKGKIV+CERG ISRTEKGEQVK
Sbjct: 361 SLYYGKSINELPLVYNNTAGDGQETNVCIAGSLDPSMVKGKIVICERGTISRTEKGEQVK 420

Query: 421 LAGGAGMILINTEFEGEELFADPHILPATTLGALAGKAILDYTASSKTQAKALIVFEGTK 480
           LAGG GMILINT+FEGEELFADPH+LPATTLGA AGKAILDY ASSKTQAKA IVFEGTK
Sbjct: 421 LAGGTGMILINTQFEGEELFADPHVLPATTLGASAGKAILDYIASSKTQAKASIVFEGTK 480

Query: 481 YGSQAPRVAAFSSRGPSLVGPDVIKPDVTAPGVNILAAWPPIVSPSELESDTRRVLFNII 540
           YGSQAPRVAAFSSRGPSLVGPDVIKPDVTAPGVNILAAWPPIVSPSEL SDTRRV+FNII
Sbjct: 481 YGSQAPRVAAFSSRGPSLVGPDVIKPDVTAPGVNILAAWPPIVSPSELASDTRRVMFNII 540

Query: 541 SGTSMSCPHVSGLAALLKSAHNDWSPAAIKSALMTTAYITDNKMSLISDVGQANGEPATP 600
           SGTSMSCPHVSGLAALLKSAHNDWSPAAIKSALMTTAY+TD+KMSLISDVGQANGEPATP
Sbjct: 541 SGTSMSCPHVSGLAALLKSAHNDWSPAAIKSALMTTAYVTDSKMSLISDVGQANGEPATP 600

Query: 601 FTFGSGHVDPEKASDPGLIYDITPQDYINYLCSLKYNSTQIALVSRGNFTCSSKRTVVKP 660
           FTFGSGHVDPEKASDPGLIYDITPQDYINYLCSLKYNS+QIALVSRGN TCSSKRTVVKP
Sbjct: 601 FTFGSGHVDPEKASDPGLIYDITPQDYINYLCSLKYNSSQIALVSRGNLTCSSKRTVVKP 660

Query: 661 GDLNYPSFSVFMKKKAKKVSITLKRTVTNVGISRSDYTVKINNPKGITVIVKPEKLSFGS 720
           GDLNYPSFSVFMKKKAKKVSITLKRTVTNVGI+RSDYTVKINNPKG+TVIVKPEKLSFGS
Sbjct: 661 GDLNYPSFSVFMKKKAKKVSITLKRTVTNVGITRSDYTVKINNPKGVTVIVKPEKLSFGS 720

Query: 721 LGEQLSYQVRFVSLGGKEALDTFSFGSLVWISGKYAVRSPIAVTWQ 767
           LGEQLSY+V FVSLGGKEALD FSFGSLVWISGKYAVRSPI VTWQ
Sbjct: 721 LGEQLSYKVSFVSLGGKEALDKFSFGSLVWISGKYAVRSPIVVTWQ 764

BLAST of Cucsa.234900 vs. NCBI nr
Match: gi|778730654|ref|XP_004140477.2| (PREDICTED: subtilisin-like protease SBT1.1 [Cucumis sativus])

HSP 1 Score: 1228.0 bits (3176), Expect = 0.0e+00
Identity = 613/770 (79.61%), Postives = 677/770 (87.92%), Query Frame = 1

Query: 1   MGFREVWVLLSI-MLAVSSAVVDQQTYIIHMDATKMVTPIPEQWYTDIIDSVNKLSSL-- 60
           MGF+EV +LL I ML  SS  +DQQTYI+HMD TKM TP PEQWYT IIDSVN+LSSL  
Sbjct: 1   MGFKEVLLLLYITMLTTSSVAMDQQTYIVHMDTTKMDTPNPEQWYTAIIDSVNQLSSLYG 60

Query: 61  -DDNEEEASNAAEILYVYKTALSGFAAKLTSKKLHSLSKIPGFLAATPNELLQLHTTHSP 120
            ++++EEA NAAEILYVYKT +SGF+AKL+S+ LHSLSK+PGF+AATPNELLQLHTTHSP
Sbjct: 61  DNNDDEEALNAAEILYVYKTVISGFSAKLSSRNLHSLSKVPGFVAATPNELLQLHTTHSP 120

Query: 121 QFLGLQRDHGLWNSSNLASDIIIGLLDTGVWPEHISFQDESLSSVPLKWKGICQTGPRFS 180
           QFLGLQR HGLWNSSNLASDIIIG+LDTG+WPEHISFQD+ L  VP KWKGICQTGP FS
Sbjct: 121 QFLGLQRGHGLWNSSNLASDIIIGVLDTGIWPEHISFQDKGLPPVPSKWKGICQTGPNFS 180

Query: 181 SSNCNKKLIGASFYIKGYEAIVGRLNETGIFRSPRDSNGHGTHTASTAAGSIVNNASFFN 240
            SNCNKKLIGA  +I+ YEA VGRLN TGIFRS RDSNGHGTHTASTAAG+ +N ASF+N
Sbjct: 181 HSNCNKKLIGARTFIQAYEAAVGRLNGTGIFRSARDSNGHGTHTASTAAGNFINRASFYN 240

Query: 241 QGMGVASGIRFTSRIVAYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGGSSSFYKD 300
           QGMGVA+G+RFTSRI +YKVCWP GCA+ADILAAMD AVADGVDVLS+SLGGGSS  Y D
Sbjct: 241 QGMGVATGMRFTSRIASYKVCWPEGCASADILAAMDHAVADGVDVLSISLGGGSSIIYSD 300

Query: 301 NIAIAAFGAIEKGVFVSCSAGNSGPSPSTVGNAAPWIMTVAASYTDRTFPTTVKLGNGQV 360
            IAIAAFGAI+KGVFVSCSAGNSGP  STV N APW+MTVAASYTDRTFPTTV+LGNG+V
Sbjct: 301 QIAIAAFGAIQKGVFVSCSAGNSGPFISTVSNVAPWVMTVAASYTDRTFPTTVRLGNGKV 360

Query: 361 FEGSSLYYGKSINELPLVYNNTAGDGQETNFCIAGSLDPSMVKGKIVVCERGQISRTEKG 420
           FEGSS Y+GK++ E+PLVYNNTAGDGQETNFC AGSLDP+MV+GKIVVCERG  SRT+KG
Sbjct: 361 FEGSSSYFGKNLKEVPLVYNNTAGDGQETNFCTAGSLDPTMVRGKIVVCERGTNSRTKKG 420

Query: 421 EQVKLAGGAGMILINTEFEGEELFADPHILPATTLGALAGKAILDYTASSKTQAKALIVF 480
           EQVKLAGGAGMILINT  EGE+L AD H+LPAT++GA A K+IL+Y ASSK QAKA I+F
Sbjct: 421 EQVKLAGGAGMILINTILEGEDLLADSHVLPATSVGASAAKSILNYIASSKRQAKASIIF 480

Query: 481 EGTKYGSQAPRVAAFSSRGPSLVGPDVIKPDVTAPGVNILAAWPPIVSPSELESDTRRVL 540
           +GTKYGS+APRVAAFSSRGPS   P VIKPD+TAPGVNILAAWPPIVSPSELESD RRVL
Sbjct: 481 KGTKYGSRAPRVAAFSSRGPSFFKPYVIKPDITAPGVNILAAWPPIVSPSELESDKRRVL 540

Query: 541 FNIISGTSMSCPHVSGLAALLKSAHNDWSPAAIKSALMTTAYITDNKMSLISDVGQANGE 600
           FNIISGTSMSCPHVSGLAAL+KS H DWSPAAIKSALMTTAY+TDNK  LISDVG+A+G 
Sbjct: 541 FNIISGTSMSCPHVSGLAALVKSVHKDWSPAAIKSALMTTAYVTDNKKHLISDVGRASGG 600

Query: 601 PATPFTFGSGHVDPEKASDPGLIYDITPQDYINYLCSLKYNSTQIALVSRGNFTCSSKRT 660
           PA  F FGSGHVDPEKAS PGLIYDI PQDYI YLCSLKY STQI+LVSRG FTCSSK T
Sbjct: 601 PADSFAFGSGHVDPEKASHPGLIYDIAPQDYITYLCSLKYTSTQISLVSRGKFTCSSKNT 660

Query: 661 VVKPGDLNYPSFSVFMKKKAKKVSITLKRTVTNVGISRSDYTVKINNPKGITVIVKPEKL 720
             +PGDLNYPSFSVFM KK K V+ T KRTVTNVGI RSDYTV+INNPKGI +IVKPEKL
Sbjct: 661 FSQPGDLNYPSFSVFM-KKGKNVNSTFKRTVTNVGIPRSDYTVRINNPKGIRIIVKPEKL 720

Query: 721 SFGSLGEQLSYQVRFVSLGGKEALDTFSFGSLVWISGKYAVRSPIAVTWQ 767
           +F  LGE+LSY+V F +LG +E+LD FSFGSLVW SG YAVRSPIAVTWQ
Sbjct: 721 NFVKLGEKLSYKVSFYALGKRESLDEFSFGSLVWHSGTYAVRSPIAVTWQ 769

BLAST of Cucsa.234900 vs. NCBI nr
Match: gi|659109542|ref|XP_008454764.1| (PREDICTED: subtilisin-like protease [Cucumis melo])

HSP 1 Score: 1225.3 bits (3169), Expect = 0.0e+00
Identity = 613/769 (79.71%), Postives = 671/769 (87.26%), Query Frame = 1

Query: 1   MGFREVWVLLSI-MLAVSSAVVDQQTYIIHMDATKMVTPIPEQWYTDIIDSVNKLSSLD- 60
           MGF+EV + L I ML  S   +DQQ+YI+HMD TKM T  PEQWYT II SVNKLSSLD 
Sbjct: 1   MGFKEVLLFLYITMLTTSIVAMDQQSYIVHMDTTKMATTNPEQWYTAIIHSVNKLSSLDA 60

Query: 61  -DNEEEASNAAEILYVYKTALSGFAAKLTSKKLHSLSKIPGFLAATPNELLQLHTTHSPQ 120
            +NEE+A N AEILYVYKT +SGF AKL+SK LH LSK+PGF+AA+PNELLQLHTTHSP+
Sbjct: 61  NNNEEQALNIAEILYVYKTVISGFCAKLSSKNLHFLSKVPGFIAASPNELLQLHTTHSPK 120

Query: 121 FLGLQRDHGLWNSSNLASDIIIGLLDTGVWPEHISFQDESLSSVPLKWKGICQTGPRFSS 180
           FLGLQR HGLWNSSNLASDIIIG+LDTG+WPEHISFQD++L  VP KWKGICQTGP FS 
Sbjct: 121 FLGLQRGHGLWNSSNLASDIIIGVLDTGIWPEHISFQDKALPPVPSKWKGICQTGPSFSL 180

Query: 181 SNCNKKLIGASFYIKGYEAIVGRLNETGIFRSPRDSNGHGTHTASTAAGSIVNNASFFNQ 240
           SNCNKKLIGA  +I+ YEA VGRLN TG FRS RDS+GHGTHTASTAAG+ VN ASF+NQ
Sbjct: 181 SNCNKKLIGARTFIQAYEAAVGRLNATGTFRSARDSDGHGTHTASTAAGNFVNRASFYNQ 240

Query: 241 GMGVASGIRFTSRIVAYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGGSSSFYKDN 300
           GMGVA+G+RFTSRI AYKVCWP GCA+ADILAAMD AVADGVDVLS+SLGGGSS  Y D 
Sbjct: 241 GMGVATGMRFTSRIAAYKVCWPEGCASADILAAMDHAVADGVDVLSISLGGGSSIIYSDQ 300

Query: 301 IAIAAFGAIEKGVFVSCSAGNSGPSPSTVGNAAPWIMTVAASYTDRTFPTTVKLGNGQVF 360
           IAIAAFGAI+KGVFVSCSAGNSGP  STVGN APW+MTVAASYTDRTFPTTV+LGNG VF
Sbjct: 301 IAIAAFGAIQKGVFVSCSAGNSGPFISTVGNVAPWVMTVAASYTDRTFPTTVRLGNGMVF 360

Query: 361 EGSSLYYGKSINELPLVYNNTAGDGQETNFCIAGSLDPSMVKGKIVVCERGQISRTEKGE 420
           EGSSLY+GK++ E PLVYNNTAGDG+ETNFC  GSLDP+MVKGKI VCERG  SRTEKGE
Sbjct: 361 EGSSLYFGKNLKETPLVYNNTAGDGRETNFCTPGSLDPTMVKGKIAVCERGTNSRTEKGE 420

Query: 421 QVKLAGGAGMILINTEFEGEELFADPHILPATTLGALAGKAILDYTASSKTQAKALIVFE 480
           QVKLAGGAGMILINT  EGE+L AD H+LPAT++G  AGK+IL+Y ASSK QAKA I+F+
Sbjct: 421 QVKLAGGAGMILINTILEGEDLLADSHVLPATSVGVSAGKSILNYIASSKRQAKASIMFK 480

Query: 481 GTKYGSQAPRVAAFSSRGPSLVGPDVIKPDVTAPGVNILAAWPPIVSPSELESDTRRVLF 540
           GTKYGS+APRVAAFSSRGPS   P VIKPD+TAPGVNILAAWPPIVSPSELESD RRVLF
Sbjct: 481 GTKYGSRAPRVAAFSSRGPSFYKPYVIKPDITAPGVNILAAWPPIVSPSELESDKRRVLF 540

Query: 541 NIISGTSMSCPHVSGLAALLKSAHNDWSPAAIKSALMTTAYITDNKMSLISDVGQANGEP 600
           NIISGTSMSCPHVSGLAALLKS H DWSPAAIKSALMTTAY+TDNK  LISDVG+A+G P
Sbjct: 541 NIISGTSMSCPHVSGLAALLKSVHKDWSPAAIKSALMTTAYVTDNKNHLISDVGRASGGP 600

Query: 601 ATPFTFGSGHVDPEKASDPGLIYDITPQDYINYLCSLKYNSTQIALVSRGNFTCSSKRTV 660
           A PF FGSGHVDPEKASDPGL+YDI PQDYI YLCSLKYNSTQIALVSRG FTCSSKRT 
Sbjct: 601 ADPFAFGSGHVDPEKASDPGLVYDIAPQDYITYLCSLKYNSTQIALVSRGKFTCSSKRTF 660

Query: 661 VKPGDLNYPSFSVFMKKKAKKVSITLKRTVTNVGISRSDYTVKINNPKGITVIVKPEKLS 720
            +PGDLNYPSFSVFM KK K V+ T KRTVTNVGI RSDYTV+I NPKGI +IVKPEKLS
Sbjct: 661 FQPGDLNYPSFSVFM-KKGKNVNSTFKRTVTNVGIPRSDYTVRIYNPKGIRIIVKPEKLS 720

Query: 721 FGSLGEQLSYQVRFVSLGGKEALDTFSFGSLVWISGKYAVRSPIAVTWQ 767
           F  LGE+LSY+V FV+LG +++LD FSFGSLVW SG Y VRSPIAVTWQ
Sbjct: 721 FVKLGEKLSYKVSFVALGKRKSLDDFSFGSLVWHSGTYVVRSPIAVTWQ 768

BLAST of Cucsa.234900 vs. NCBI nr
Match: gi|743788982|ref|XP_011035007.1| (PREDICTED: subtilisin-like protease [Populus euphratica])

HSP 1 Score: 996.5 bits (2575), Expect = 2.6e-287
Identity = 496/772 (64.25%), Postives = 604/772 (78.24%), Query Frame = 1

Query: 1   MGFREVWVLLSIMLAVSSAV-VDQQTYIIHMDATKMVT---PI--PEQWYTDIIDSVNKL 60
           M FR   +LL+ M A +SA  +D+QTYIIHMD  KM     P+    QWY  +IDS+ + 
Sbjct: 1   MIFRISLLLLAFMAAKASAASIDKQTYIIHMDKNKMPALYDPLGNSRQWYESVIDSITQF 60

Query: 61  SSLDDNEEEASNAAEILYVYKTALSGFAAKLTSKKLHSLSKIPGFLAATPNELLQLHTTH 120
           SS +  EE+ +   ++LY Y+T  SGFAAKL++K++ +LS++ GFL+A P+ +L LHTTH
Sbjct: 61  SSQEQEEEQETGFPQLLYTYETVTSGFAAKLSTKQVEALSRVDGFLSAIPDGMLTLHTTH 120

Query: 121 SPQFLGLQRDHGLWNSSNLASDIIIGLLDTGVWPEHISFQDESLSSVPLKWKGICQTGPR 180
           +PQFLGLQ   GLWN+ NLASD+I+G+LDTG+WPEH+SFQD  +S+VPLKWKG C++G +
Sbjct: 121 TPQFLGLQSGKGLWNAQNLASDVIVGILDTGIWPEHVSFQDSGMSAVPLKWKGKCESGTK 180

Query: 181 FSSSNCNKKLIGASFYIKGYEAIVGRLNETGIFRSPRDSNGHGTHTASTAAGSIVNNASF 240
           FS SNCNKKLIGA  + KGYE+I GR+NET  +RSPRDS GHGTHTA+TAAG++V+ ASF
Sbjct: 181 FSPSNCNKKLIGARAFFKGYESIAGRINETVDYRSPRDSQGHGTHTAATAAGNLVDKASF 240

Query: 241 FNQGMGVASGIRFTSRIVAYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGGSSSFY 300
           +    G A+G+++T+RI AYKVCW  GC N D+LAAMD AVADGVDVLSLSLGG +  FY
Sbjct: 241 YGLANGSAAGMKYTARIAAYKVCWTSGCTNTDLLAAMDQAVADGVDVLSLSLGGSAKPFY 300

Query: 301 KDNIAIAAFGAIEKGVFVSCSAGNSGPSPSTVGNAAPWIMTVAASYTDRTFPTTVKLGNG 360
            D++AIA+FGAI+KGVFVSCSAGNSGPS S+V N APWIMTVAASYTDR FPTTVKLGNG
Sbjct: 301 SDSVAIASFGAIQKGVFVSCSAGNSGPSISSVDNNAPWIMTVAASYTDRRFPTTVKLGNG 360

Query: 361 QVFEGSSLYYGKSINELPLVYNNTAGDGQETNFCIAGSLDPSMVKGKIVVCERGQISRTE 420
           Q FEG+SLY GK+  +LPLV+  TAG G+   +CI GSL   +VKGK+VVC+RG   R +
Sbjct: 361 QSFEGASLYTGKATAQLPLVHAGTAG-GEGAEYCITGSLKRKLVKGKMVVCKRGMSGRAQ 420

Query: 421 KGEQVKLAGGAGMILINTEFEGEELFADPHILPATTLGALAGKAILDYTASSKTQAKALI 480
           KGEQVKLAGG GM+LINTE  GEELFAD H+LPAT+LGA AG A+  Y  S+K +A A I
Sbjct: 421 KGEQVKLAGGTGMLLINTETGGEELFADAHVLPATSLGASAGMAVKGYMNSTK-RATASI 480

Query: 481 VFEGTKYGSQAPRVAAFSSRGPSLVGPDVIKPDVTAPGVNILAAWPPIVSPSELESDTRR 540
            F+GT YG+ AP +AAFSSRGPS VGPDVIKPDVTAPG+NILAAWPP+ SP+ L+SD R 
Sbjct: 481 AFKGTVYGNPAPMLAAFSSRGPSSVGPDVIKPDVTAPGMNILAAWPPMTSPTLLKSDKRS 540

Query: 541 VLFNIISGTSMSCPHVSGLAALLKSAHNDWSPAAIKSALMTTAYITDNKMSLISDVGQAN 600
           VLFN+ISGTSMSCPHVSGLAALLKS H  WSPAAIKSALMTTAY TDN+ S I+D G +N
Sbjct: 541 VLFNVISGTSMSCPHVSGLAALLKSVHKKWSPAAIKSALMTTAYATDNRGSPIADAGSSN 600

Query: 601 GEPATPFTFGSGHVDPEKASDPGLIYDITPQDYINYLCSLKYNSTQIALVSRGNFTCSSK 660
              ATPF FGSGHVDPE+ASDPGLIYDIT +DY+NY CSL Y S+QIA VSR N TC   
Sbjct: 601 SASATPFAFGSGHVDPERASDPGLIYDITIEDYLNYFCSLDYTSSQIAQVSRRNVTCPDN 660

Query: 661 RTVVKPGDLNYPSFSVFMKKKAKKVSITLKRTVTNVGISRSDYTVKINNPKGITVIVKPE 720
           +  ++PGDLNYPSF+V  +  A+   +  KRT+TNVG   S Y VK+  P G++VI++P+
Sbjct: 661 K-ALQPGDLNYPSFAVNFEGNARNNRVKYKRTLTNVGTPSSTYAVKVEEPNGVSVILEPK 720

Query: 721 KLSFGSLGEQLSYQVRFVSLGGKEALDTFSFGSLVWISGKYAVRSPIAVTWQ 767
            LSF  LG++LSY V FVS GGK    + S GSLVW+SGKY+VRSPIAVTWQ
Sbjct: 721 SLSFEKLGQKLSYNVTFVSSGGKGREGSSSIGSLVWLSGKYSVRSPIAVTWQ 769