Cucsa.226930 (gene) Cucumber (Gy14) v1

NameCucsa.226930
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionProtein disulfide-isomerase
Locationscaffold01910 : 70460 .. 74933 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTCTTCTTCACGATGGCTACTCGAACTCTTCTTCTTCTTGCTATGGCTGCTCTTCTTCTCTTCTCCAACTCCGTTCTTTGTAAAGATGATTCTCCTCCCAAAGATCCTCTCTCCAAGCCTACCGACGACGATGACGAAGATCTAAGCTTCCTTGAGGAGCCGGATGAGGCTCATGCTCATCAGAATCTCCCTGATTTTGATAATTTTGAAGGGGGAGCTGAGGATGAAGATTTTGGAGACTTCACCGATTTTGAAGATGCAGAAGGTGATGGAGACGAGTACAAGGCACCGGTGGTGGATGAAAAGGACGTTGTTGTCTTGAAGGAAGGTAACTTCAGCGATTTCATCAAGAAGAATCGGTTTGTTATGGTGGAGTTTTATGCTCCATGGTGTGGTCATTGCCAAGCGTTGGCTCCTGAATACGCGGCTGCGGCTACTGAATTGAAGGCGGAGAATGTGGCTTTGGCGAAGGTTGATGCAACAGAGGAGAATGAATTGGCGCAGCAGTACGATGTTCAAGGATTTCCTACCGTTTATTTCTTCTCTGATGGTGTCCACAAGGCTTATCCTGGACAGAGGACAAAGTAAGTGCTCTGAATTGTTAAGACTTGTTTGGCGGTCATTGATGCTTCGGAAATGGAGCAAATGCTCGACTTCAGATTTGTTTTCAACTTCGAGATCTAGATAGTAGAACTGTTTGCCACAATGCGTTAATAAATCAGCGTTATATTACATTCTTTATTTGTGTGTTCACCGTTCCCTGTTTAAATACGCATTTAATGTTTAAGCTAATTATCACGTCTTCACAGGGATGCTATAGTATCATGGATCAAGAAGAAGACCGGACCTGGTATTTACAACATAACTTCGGTTGAAGATGCTGAACGCATTCTGACTTCGGAATCTAAGGTCGCTGTTGGTTACCTGAACTCCTTGGTGGTGAGTTTGTTTACACGTTTCTTTATATGCTTGGACTTATCACCTTTGATTGGAAATGGAATTTACATTTCTTCGATCGACTTCATGAACTGTACTTTGCTATAGTGCTAGTGGGTCAATGGATAAATGTATAGGGATGCTTATTATTTTCTTCATGATCTTGTGTTGCTATTTGCAGGGCTCTGAGAGTGATGAGCTTGCTGCTGCTTCCAGACTGGAAGATGATGTCAACTTTTACCAAACGGTGGATCCTGAAGTGGCAAAGCTTTTCCACATTGAAGCTTCCGCAAAACGCCCTGCCTTGGTATTGCTTAAGAAGGAGGCTGAAAAACTGAGCCGCTTTGGTTAGATCATTGCTTCTGTTTTCTTAATTACTTTAAGAGATGATATACTTTGGGTGGGTAGTAATTTAGTGAGTTTTGTTTCTTTTGCAGATGGCGAGTTTTCGAAGTCTGCAATTGTTGAATTTGTATTTGCCAATAAGCTTCCTTTAGTTACAATGTTTACTAAAGAAAATGCACCATTGATTTTTGAAAGTTCAATTAAGAAACAGGTATTGTAGTATAAAATGGGCTTTAGCTATCACTTTGAAACAACTTTTGTTTGTGAAGTTGAGCTCAAGAATTCCTTTGCACTTGACACTTTGTATTTTTCTGTGGCCAGTTAATTCTATTTGCAATTTCAAATGACACAGAGAAACTCATCCCCATATTTGAAGAAGCAGCCAAGTCTTTTAAAGGAAAGGTATAGTTGTTGCTAAGTAAAGGATAATTTTTTTTTAGAATTGAGAAGCTAACTTCAAATTCGAAAGCTTAGTCTCAGTAACTGGGAATATTTTGTTGTCCTTAGCTTGTACATTCAGCAAACTGTCTTATTTTTCATGTAGATATTATTTTTATTAATGAGGAATTGTGAGATGCATTGTTATACTATTTTGGAATTCAGGGTTGCTCTCTGTATTTCCTTCTAATTTTATGTGTTTTGATGCAGCTTATTTTCGTTTATGTGGAGATTGATAATGAGGAGGTTGGAAAGCCAGTATCAGAATATTTTGGCGTTAATGGCAATGGTCCAGAGGTGATCTTTTTCATTACCTCTAATATATTCATTTTCAAATAAAACTTTAGTACGTTTGTTTAACTTTAGCAAGCTTGATATTGGGAAGTTTAAATTCGTATTATGCATTTGGATTATATGAAAAATAGGTTTAAATTCGTATTATGCATTTGGATTATATGAAAAATAGGTTTATGTAATCTATGTTTGTTTCGTGCGTGTATGGTTTGTAAAATTTAGAATATACTCGATAGTACATATTATATAATGTGTGAGCTGTCCTTTTCATGTATTTCCGAAGTTAAACTTGCCTACCTATAAACATCAATGTCCATTGAATGTGTTGTACTTAGAGTTGATATTTTATAGAAATCAAACGAACACGAAACTGATTTTACAAATAGGCTTTGATATTGAAAACATAGAAAACATTTTCAATTTTCATGAATGTATGCGTCCCAATTTTAAAAACAAGTTCTACTTCACCGGAAATGGGGACAGCCAGAAATCTGTGCTTGATTAGGAAGTTTGGTTGAATTTGGTTGTTAGTATATTCATATGTTGTGATGAAATTGAAAATTTTATTTTCATATAGGTTCTTGGATACACTGGAAATGAGGACAGCAAGAAATTCGTGCTTGATAAGGAAGTTACCTTGGAAAATATTAAGGTCCACCAATTAATCTTCCTTCCTATCCCTGTAAAGAGTATTCTTTTTACTTCAACTTCTCAGCTAATTTCTCTTTTCTCATCCAATTCACTGGACAGGCTTTTGCAGAAAATTTCTTGGAAGACAAGCTAAAACCCTTCTATAAGTCAGATCCCATTCCCGAGACTGTAAGTTGAACTAACACACTTTTCATTCAATGAAAAGTTTTTGTTATCTTTTTTTTTAAAAAAAAAAGTTGAATTGACTCACATTTCCTGTCAGTATGACTTTACTTTTCGTATTTGATCTCTAAAATGTTTACCTCTCTATTCATGCAGAATGATGGTGACGTGAAAATAGTGGTTGGAGACAACTTTGATGAAATTGTTTTAGATGAATCGAAGGATGTTCTCCTTGAGGTATTGAGTAATCCAACAAGCATTTTATCTGTTGATCCTCTCATGTTGTTCGTTGTATACTGATTTATTTTTCATTTCTAGATTTATGCTCCTTGGTGTGGGCATTGCCAAGCATTGGAACCAACTTACAACAAGCTTGCGAAACATCTACATGGCGTCGATTCACTCGTCATTGCTAAGATGGATGGCACAACAAATGAACATCCCCGGGCGAAGGTATGGAGATTTCATTGTCCCCTTTCTGCATTTGTTGAGTTCATAATCAATTTCCAAATACTTTCTTTTTCCCCCCAAACCTGGATTTTGAAACTACTCTTGAAAAGTAGAAAGCAAAATAAAAAGAAGATGTGGTATTGTAAGTTTAGTTTGAAAGAACCACATACCAAATTATACACTAACATATATATGGGATCATTGCGAATATAGCAATCAAGTTCAAATATTGAATATAGTAATCAAGTCCAAATATTGAATATAGCAATCAAGTCCAAATATTGAATATAGCAATCAAGTCCAAAATATTTGAAGATATAGCACAATGTAAAAGAATTTGCAAATATGACAATTTAGATTAAGTTGTTGAAATCTTCTGTGACTATCACTACTCGTAGTATAATTATATTGTTATATTTGCAGCTTTTCTAAAATGCAGCTATATACTTAATTATTATCTCTAGACGTGATACCAAACACAATTATATATTGCACTGACAAAACAAGTGATCTTTTGAACCTGTGATGATGTTCAACTCAAACTGATTAGAAAATTGCTGCTTGTTTTTCTTCTTGTAACAGTCGGATGGATTCCCAACAATTCTATTCTTCCCTGCTGGAAATAAGAGCTTTGATCCTGTAAGTAAACAAAAACATATTCGTAATCTCTTTAATTCTTAGTCTCTTGATTCTTCCACCTTGACGTCACTTCCTCCCAATAAGTAATCAAATCCGTTTCTGGCTGCTTGGTTTGATATCTAGATCACGGTTGATACGGATCGGACGGTTGTAGCATTTTACAAATTCCTAAAGAAAAATGCATCAATCCCTTTTAAGCTACAGAAGCCAGTTTCAAGTCCAAAAGCTGAAAGTTCTGAAGGCAAATCTAGTGATGATGCAAAAGAGAGCCCAAAGAGCACTGACTTAAAGGATGAATTGTGAAGACTTACTATAGTTCTGTAAGTTATGCATCTCTTATTTCCCCTTTTTTATTGCTTTGCAGATAATGTAGACGATAAATACGAGAGACTAGAAATTATAAGAATACCTTTTTTTCCCTATTAGAGAAGTCTTCGTAAAGAAACCACTCTTAATTTCCATTATTTCTTAATAACAAGAAAGAAGAAGAGACGAGTCCTTTGGTTTTGTAGTGTATGCTGTAAGGAAAAAAAAAGATAATTGTAGGTAGTGGAATTG

mRNA sequence

ATTCTTCTTCACGATGGCTACTCGAACTCTTCTTCTTCTTGCTATGGCTGCTCTTCTTCTCTTCTCCAACTCCGTTCTTTGTAAAGATGATTCTCCTCCCAAAGATCCTCTCTCCAAGCCTACCGACGACGATGACGAAGATCTAAGCTTCCTTGAGGAGCCGGATGAGGCTCATGCTCATCAGAATCTCCCTGATTTTGATAATTTTGAAGGGGGAGCTGAGGATGAAGATTTTGGAGACTTCACCGATTTTGAAGATGCAGAAGGTGATGGAGACGAGTACAAGGCACCGGTGGTGGATGAAAAGGACGTTGTTGTCTTGAAGGAAGGTAACTTCAGCGATTTCATCAAGAAGAATCGGTTTGTTATGGTGGAGTTTTATGCTCCATGGTGTGGTCATTGCCAAGCGTTGGCTCCTGAATACGCGGCTGCGGCTACTGAATTGAAGGCGGAGAATGTGGCTTTGGCGAAGGTTGATGCAACAGAGGAGAATGAATTGGCGCAGCAGTACGATGTTCAAGGATTTCCTACCGTTTATTTCTTCTCTGATGGTGTCCACAAGGCTTATCCTGGACAGAGGACAAAGGATGCTATAGTATCATGGATCAAGAAGAAGACCGGACCTGGTATTTACAACATAACTTCGGTTGAAGATGCTGAACGCATTCTGACTTCGGAATCTAAGGTCGCTGTTGGTTACCTGAACTCCTTGGTGGGCTCTGAGAGTGATGAGCTTGCTGCTGCTTCCAGACTGGAAGATGATGTCAACTTTTACCAAACGGTGGATCCTGAAGTGGCAAAGCTTTTCCACATTGAAGCTTCCGCAAAACGCCCTGCCTTGGTATTGCTTAAGAAGGAGGCTGAAAAACTGAGCCGCTTTGTTACAATGTTTACTAAAGAAAATGCACCATTGATTTTTGAAAGTTCAATTAAGAAACAGTTAATTCTATTTGCAATTTCAAATGACACAGAGAAACTCATCCCCATATTTGAAGAAGCAGCCAAGTCTTTTAAAGGAAAGCTTATTTTCGTTTATGTGGAGATTGATAATGAGGAGGTTGGAAAGCCAGTATCAGAATATTTTGGCGTTAATGGCAATGGTCCAGAGGTTCTTGGATACACTGGAAATGAGGACAGCAAGAAATTCGTGCTTGATAAGGAAGTTACCTTGGAAAATATTAAGGCTTTTGCAGAAAATTTCTTGGAAGACAAGCTAAAACCCTTCTATAAGTCAGATCCCATTCCCGAGACTAATGATGGTGACGTGAAAATAGTGGTTGGAGACAACTTTGATGAAATTGTTTTAGATGAATCGAAGGATGTTCTCCTTGAGATTTATGCTCCTTGGTGTGGGCATTGCCAAGCATTGGAACCAACTTACAACAAGCTTGCGAAACATCTACATGGCGTCGATTCACTCGTCATTGCTAAGATGGATGGCACAACAAATGAACATCCCCGGGCGAAGTCGGATGGATTCCCAACAATTCTATTCTTCCCTGCTGGAAATAAGAGCTTTGATCCTATCACGGTTGATACGGATCGGACGGTTGTAGCATTTTACAAATTCCTAAAGAAAAATGCATCAATCCCTTTTAAGCTACAGAAGCCAGTTTCAAGTCCAAAAGCTGAAAGTTCTGAAGGCAAATCTAGTGATGATGCAAAAGAGAGCCCAAAGAGCACTGACTTAAAGGATGAATTGTGAAGACTTACTATAGTTCTGTAAGTTATGCATCTCTTATTTCCCCTTTTTTATTGCTTTGCAGATAATGTAGACGATAAATACGAGAGACTAGAAATTATAAGAATACCTTTTTTTCCCTATTAGAGAAGTCTTCGTAAAGAAACCACTCTTAATTTCCATTATTTCTTAATAACAAGAAAGAAGAAGAGACGAGTCCTTTGGTTTTGTAGTGTATGCTGTAAGGAAAAAAAAAGATAATTGTAGGTAGTGGAATTG

Coding sequence (CDS)

ATGGCTACTCGAACTCTTCTTCTTCTTGCTATGGCTGCTCTTCTTCTCTTCTCCAACTCCGTTCTTTGTAAAGATGATTCTCCTCCCAAAGATCCTCTCTCCAAGCCTACCGACGACGATGACGAAGATCTAAGCTTCCTTGAGGAGCCGGATGAGGCTCATGCTCATCAGAATCTCCCTGATTTTGATAATTTTGAAGGGGGAGCTGAGGATGAAGATTTTGGAGACTTCACCGATTTTGAAGATGCAGAAGGTGATGGAGACGAGTACAAGGCACCGGTGGTGGATGAAAAGGACGTTGTTGTCTTGAAGGAAGGTAACTTCAGCGATTTCATCAAGAAGAATCGGTTTGTTATGGTGGAGTTTTATGCTCCATGGTGTGGTCATTGCCAAGCGTTGGCTCCTGAATACGCGGCTGCGGCTACTGAATTGAAGGCGGAGAATGTGGCTTTGGCGAAGGTTGATGCAACAGAGGAGAATGAATTGGCGCAGCAGTACGATGTTCAAGGATTTCCTACCGTTTATTTCTTCTCTGATGGTGTCCACAAGGCTTATCCTGGACAGAGGACAAAGGATGCTATAGTATCATGGATCAAGAAGAAGACCGGACCTGGTATTTACAACATAACTTCGGTTGAAGATGCTGAACGCATTCTGACTTCGGAATCTAAGGTCGCTGTTGGTTACCTGAACTCCTTGGTGGGCTCTGAGAGTGATGAGCTTGCTGCTGCTTCCAGACTGGAAGATGATGTCAACTTTTACCAAACGGTGGATCCTGAAGTGGCAAAGCTTTTCCACATTGAAGCTTCCGCAAAACGCCCTGCCTTGGTATTGCTTAAGAAGGAGGCTGAAAAACTGAGCCGCTTTGTTACAATGTTTACTAAAGAAAATGCACCATTGATTTTTGAAAGTTCAATTAAGAAACAGTTAATTCTATTTGCAATTTCAAATGACACAGAGAAACTCATCCCCATATTTGAAGAAGCAGCCAAGTCTTTTAAAGGAAAGCTTATTTTCGTTTATGTGGAGATTGATAATGAGGAGGTTGGAAAGCCAGTATCAGAATATTTTGGCGTTAATGGCAATGGTCCAGAGGTTCTTGGATACACTGGAAATGAGGACAGCAAGAAATTCGTGCTTGATAAGGAAGTTACCTTGGAAAATATTAAGGCTTTTGCAGAAAATTTCTTGGAAGACAAGCTAAAACCCTTCTATAAGTCAGATCCCATTCCCGAGACTAATGATGGTGACGTGAAAATAGTGGTTGGAGACAACTTTGATGAAATTGTTTTAGATGAATCGAAGGATGTTCTCCTTGAGATTTATGCTCCTTGGTGTGGGCATTGCCAAGCATTGGAACCAACTTACAACAAGCTTGCGAAACATCTACATGGCGTCGATTCACTCGTCATTGCTAAGATGGATGGCACAACAAATGAACATCCCCGGGCGAAGTCGGATGGATTCCCAACAATTCTATTCTTCCCTGCTGGAAATAAGAGCTTTGATCCTATCACGGTTGATACGGATCGGACGGTTGTAGCATTTTACAAATTCCTAAAGAAAAATGCATCAATCCCTTTTAAGCTACAGAAGCCAGTTTCAAGTCCAAAAGCTGAAAGTTCTGAAGGCAAATCTAGTGATGATGCAAAAGAGAGCCCAAAGAGCACTGACTTAAAGGATGAATTGTGA

Protein sequence

MATRTLLLLAMAALLLFSNSVLCKDDSPPKDPLSKPTDDDDEDLSFLEEPDEAHAHQNLPDFDNFEGGAEDEDFGDFTDFEDAEGDGDEYKAPVVDEKDVVVLKEGNFSDFIKKNRFVMVEFYAPWCGHCQALAPEYAAAATELKAENVALAKVDATEENELAQQYDVQGFPTVYFFSDGVHKAYPGQRTKDAIVSWIKKKTGPGIYNITSVEDAERILTSESKVAVGYLNSLVGSESDELAAASRLEDDVNFYQTVDPEVAKLFHIEASAKRPALVLLKKEAEKLSRFVTMFTKENAPLIFESSIKKQLILFAISNDTEKLIPIFEEAAKSFKGKLIFVYVEIDNEEVGKPVSEYFGVNGNGPEVLGYTGNEDSKKFVLDKEVTLENIKAFAENFLEDKLKPFYKSDPIPETNDGDVKIVVGDNFDEIVLDESKDVLLEIYAPWCGHCQALEPTYNKLAKHLHGVDSLVIAKMDGTTNEHPRAKSDGFPTILFFPAGNKSFDPITVDTDRTVVAFYKFLKKNASIPFKLQKPVSSPKAESSEGKSSDDAKESPKSTDLKDEL*
BLAST of Cucsa.226930 vs. Swiss-Prot
Match: PDI14_ARATH (Protein disulfide isomerase-like 1-4 OS=Arabidopsis thaliana GN=PDIL1-4 PE=1 SV=1)

HSP 1 Score: 776.2 bits (2003), Expect = 2.6e-223
Identity = 389/605 (64.30%), Postives = 472/605 (78.02%), Query Frame = 1

Query: 1   MATRTLLLLAMAALLLFSNSVLCKDDSPPKDPLSKPTDDDDEDLSFLEEPDE-----AHA 60
           MA R LLL ++ ALL+FS          P    S   D DDEDLSFLE+  E     A +
Sbjct: 1   MAFRVLLLFSLTALLIFSAV-------SPSFAASSSDDVDDEDLSFLEDLKEDDVPGADS 60

Query: 61  HQNLPDFDNFEGGAEDEDFGDFTDFEDAEGD--------GDEYKAPVVDEKDVVVLKEGN 120
             +   FD FEGG E+ED   + D +D EGD         D    P +DEKDVVV+KE N
Sbjct: 61  LSSSTGFDEFEGG-EEEDPDMYNDDDDEEGDFSDLGNPDSDPLPTPEIDEKDVVVIKERN 120

Query: 121 FSDFIKKNRFVMVEFYAPWCGHCQALAPEYAAAATELKAENVALAKVDATEENELAQQYD 180
           F+D I+ N++V+VEFYAPWCGHCQ+LAPEYAAAATELK + V LAK+DATEENELAQ+Y 
Sbjct: 121 FTDVIENNQYVLVEFYAPWCGHCQSLAPEYAAAATELKEDGVVLAKIDATEENELAQEYR 180

Query: 181 VQGFPTVYFFSDGVHKAYPGQRTKDAIVSWIKKKTGPGIYNITSVEDAERILTSESKVAV 240
           VQGFPT+ FF DG HK Y G RTK+ IV+W+KKK GPG+YN+T+++DAE++LTS +KV +
Sbjct: 181 VQGFPTLLFFVDGEHKPYTGGRTKETIVTWVKKKIGPGVYNLTTLDDAEKVLTSGNKVVL 240

Query: 241 GYLNSLVGSESDELAAASRLEDDVNFYQTVDPEVAKLFHIEASAKRPALVLLKKEAEKLS 300
           GYLNSLVG E D+L AAS+ EDDVNFYQTV+P+VAK+FH++  +KRPALVL+KKE EK+S
Sbjct: 241 GYLNSLVGVEHDQLNAASKAEDDVNFYQTVNPDVAKMFHLDPESKRPALVLVKKEEEKIS 300

Query: 301 RFDGEFSKSAIVEFVFANKLPLVTMFTKENAPLIFESSIKKQLILFAISNDTEKLIPIFE 360
            FDGEF KSA+V FV ANKL LV++FT+E AP IFES+IKKQL+LF   N++EK++  F+
Sbjct: 301 HFDGEFVKSALVSFVSANKLALVSVFTRETAPEIFESAIKKQLLLFVTKNESEKVLTEFQ 360

Query: 361 EAAKSFKGKLIFVYVEIDNEEVGKPVSEYFGVNGNGPEVLGYTGNEDSKKFVLDKEVTLE 420
           EAAKSFKGKLIFV V++DNE+ GKPV+EYFGV+GNGP+++GYTGNED KK+  D E+  +
Sbjct: 361 EAAKSFKGKLIFVSVDLDNEDYGKPVAEYFGVSGNGPKLIGYTGNEDPKKYFFDGEIQSD 420

Query: 421 NIKAFAENFLEDKLKPFYKSDPIPETNDGDVKIVVGDNFDEIVLDESKDVLLEIYAPWCG 480
            IK F E+FL DKLKPFYKSDPIPE ND DVKIVVGDNFDEIVLD+SKDVLLE+YAPWCG
Sbjct: 421 KIKIFGEDFLNDKLKPFYKSDPIPEKNDEDVKIVVGDNFDEIVLDDSKDVLLEVYAPWCG 480

Query: 481 HCQALEPTYNKLAKHLHGVDSLVIAKMDGTTNEHPRAKSDGFPTILFFPAGNKSFDPITV 540
           HCQALEP YNKLAKHL  +DSLVI KMDGTTNEHP+AK++GFPTILFFPAGNK+ +PITV
Sbjct: 481 HCQALEPMYNKLAKHLRSIDSLVITKMDGTTNEHPKAKAEGFPTILFFPAGNKTSEPITV 540

Query: 541 DTDRTVVAFYKFLKKNASIPFKLQKPV---------SSPKAESSEGKSSDDAKESPKSTD 584
           DTDRTVVAFYKFL+K+A+IPFKL+KP          S+PK E++E K S D+      +D
Sbjct: 541 DTDRTVVAFYKFLRKHATIPFKLEKPASTESPKTAESTPKVETTETKESPDSTTKSSQSD 597

BLAST of Cucsa.226930 vs. Swiss-Prot
Match: PDI13_ARATH (Protein disulfide isomerase-like 1-3 OS=Arabidopsis thaliana GN=PDIL1-3 PE=2 SV=1)

HSP 1 Score: 698.7 bits (1802), Expect = 5.2e-200
Identity = 357/572 (62.41%), Postives = 435/572 (76.05%), Query Frame = 1

Query: 13  ALLLFSNSVLCKDDSPPKDPLSKPTDDDDEDLSFLEEPDEAHAHQNLPDFDNFEGGAEDE 72
           +LLLF + +L   +S  ++  S    D DE+L+FL   +          +   E   +  
Sbjct: 9   SLLLFVSFILLLVNSRAENASSG--SDLDEELAFLAAEESKEQSHGGGSYHEEEHDHQHR 68

Query: 73  DFGDFTDFEDAEGD---GD----EYKAPVVDEKDVVVLKEGNFSDFIKKNRFVMVEFYAP 132
           DF ++ D E   G+   GD    E   P VDEKDV VL + NF++F+  N F MVEFYAP
Sbjct: 69  DFENYDDLEQGGGEFHHGDHGYEEEPLPPVDEKDVAVLTKDNFTEFVGNNSFAMVEFYAP 128

Query: 133 WCGHCQALAPEYAAAATELKAENVALAKVDATEENELAQQYDVQGFPTVYFFSDG-VHKA 192
           WCG CQAL PEYAAAATELK    ALAK+DATEE +LAQ+Y++QGFPTV+ F DG + K 
Sbjct: 129 WCGACQALTPEYAAAATELKGL-AALAKIDATEEGDLAQKYEIQGFPTVFLFVDGEMRKT 188

Query: 193 YPGQRTKDAIVSWIKKKTGPGIYNITSVEDAERILTSESKVAVGYLNSLVGSESDELAAA 252
           Y G+RTKD IV+W+KKK  P I+NIT+ E+AER+L++E K+  G+LNSLVGSES+ELAAA
Sbjct: 189 YEGERTKDGIVTWLKKKASPSIHNITTKEEAERVLSAEPKLVFGFLNSLVGSESEELAAA 248

Query: 253 SRLEDDVNFYQTVDPEVAKLFHIEASAKRPALVLLKKEAEKLSRFDGEFSKSAIVEFVFA 312
           SRLEDD++FYQT  P++AKLF IE   KRPALVLLKKE EKL+RFDG F+K+AI EFV A
Sbjct: 249 SRLEDDLSFYQTASPDIAKLFEIETQVKRPALVLLKKEEEKLARFDGNFTKTAIAEFVSA 308

Query: 313 NKLPLVTMFTKENAPLIFESSIKKQLILFAISNDTEKLIPIFEEAAKSFKGKLIFVYVEI 372
           NK+PLV  FT+E A LIFESS+K QLILFA +N++EK +P   E AKSFKGK +FVYV++
Sbjct: 309 NKVPLVINFTREGASLIFESSVKNQLILFAKANESEKHLPTLREVAKSFKGKFVFVYVQM 368

Query: 373 DNEEVGKPVSEYFGVNGNGPEVLGYTGNEDSKKFVLDKEVTLENIKAFAENFLEDKLKPF 432
           DNE+ G+ VS +FGV G  P+VL YTGNED +KF+LD E+T+ NIK  AE+FL DKLKPF
Sbjct: 369 DNEDYGEAVSGFFGVTGAAPKVLVYTGNEDMRKFILDGELTVNNIKTLAEDFLADKLKPF 428

Query: 433 YKSDPIPETNDGDVKIVVGDNFDEIVLDESKDVLLEIYAPWCGHCQALEPTYNKLAKHLH 492
           YKSDP+PE NDGDVK++VG+NFDEIVLDESKDVLLEIYAPWCGHCQ+ EP YNKL K+L 
Sbjct: 429 YKSDPLPENNDGDVKVIVGNNFDEIVLDESKDVLLEIYAPWCGHCQSFEPIYNKLGKYLK 488

Query: 493 GVDSLVIAKMDGTTNEHPRAKSDGFPTILFFPAGNKSFDPITVDTDRTVVAFYKFLKKNA 552
           G+DSLV+AKMDGT+NEHPRAK+DGFPTILFFP GNKSFDPI VD DRTVV  YKFLKK+A
Sbjct: 489 GIDSLVVAKMDGTSNEHPRAKADGFPTILFFPGGNKSFDPIAVDVDRTVVELYKFLKKHA 548

Query: 553 SIPFKLQKP------VSSPKA-ESSEGKSSDD 570
           SIPFKL+KP      +S+ K+ E  EG SS D
Sbjct: 549 SIPFKLEKPATPEPVISTMKSDEKIEGDSSKD 577

BLAST of Cucsa.226930 vs. Swiss-Prot
Match: PDI14_ORYSJ (Protein disulfide isomerase-like 1-4 OS=Oryza sativa subsp. japonica GN=PDIL1-4 PE=2 SV=1)

HSP 1 Score: 676.0 bits (1743), Expect = 3.6e-193
Identity = 350/588 (59.52%), Postives = 434/588 (73.81%), Query Frame = 1

Query: 1   MATRTLLLLAMAALLLFSNSVLCKDDSPPKDPLSKPTDDDDEDLSFLEEPDEAHAHQNLP 60
           M +R+LLL+A+A LLL +               S    DDD D   ++  D+  A  N P
Sbjct: 1   MRSRSLLLVALATLLLHA---------------SASASDDDLDY-LIDNADDIPA--NDP 60

Query: 61  DFDNFEGGAEDEDFGDFTDFEDAEGDGDEYKAPVVDEKDVVVLKEGNFSDFIKKNRFVMV 120
           D    EG  +D+D  D      A+          +DE  V +L   NFSDF+  +R VMV
Sbjct: 61  DGWLQEGSPDDDDDDDLFHHGQAQDHP-------IDETHVFLLSAANFSDFLASHRHVMV 120

Query: 121 EFYAPWCGHCQALAPEYAAAATELK--AENVALAKVDATEENELAQQYDVQGFPTVYFFS 180
           EFYAPWC HCQALAP+YAAAA +L   A  VALAKVDATE+ +LAQ+YDVQGFPT+ FF 
Sbjct: 121 EFYAPWCAHCQALAPDYAAAAADLSPLAHQVALAKVDATEDTDLAQKYDVQGFPTILFFI 180

Query: 181 DGVHKAYPGQRTKDAIVSWIKKKTGPGIYNITSVEDAERILTSESKVAVGYLNSLVGSES 240
           DGV K Y G RTK+AIVSW+ KK  PG+ NIT+V++AE+ILT E K  +  L+SL G+ S
Sbjct: 181 DGVPKDYNGARTKEAIVSWVNKKLAPGVQNITTVDEAEKILTGEDKAILAVLDSLSGAHS 240

Query: 241 DELAAASRLEDDVNFYQTVDPEVAKLFHIEASAKRPALVLLKK-EAEKLSRFDGEFSKSA 300
           DE+AAASRLED +NFYQT +P+VAKLFH++ +AKRP+LVLLKK E EKL+ +DG F  SA
Sbjct: 241 DEIAAASRLEDAINFYQTSNPDVAKLFHLDPAAKRPSLVLLKKQEEEKLTFYDGPFKASA 300

Query: 301 IVEFVFANKLPLVTMFTKENAPLIFESSIKKQLILFAISNDTEKLIPIFEEAAKSFKGKL 360
           I +FV ANKLPLV   T+E AP IF++ IKKQ++LF ++N++ K +PIF+EA+KSFKGKL
Sbjct: 301 IADFVSANKLPLVNTLTQETAPSIFDNPIKKQILLFVVANESSKFLPIFKEASKSFKGKL 360

Query: 361 IFVYVEIDNEEVGKPVSEYFGVNGNGPEVLGYTGNEDSKKFVLDKEVTLENIKAFAENFL 420
           +FV+VE DNEEVG+PV+ YFG+ G    VL YTGNED++ F LD E+++ENIK FAE+FL
Sbjct: 361 LFVFVERDNEEVGEPVANYFGITGQETTVLAYTGNEDARNFFLDGEISVENIKRFAEDFL 420

Query: 421 EDKLKPFYKSDPIPETNDGDVKIVVGDNFDEIVLDESKDVLLEIYAPWCGHCQALEPTYN 480
           E+KL PFYKS+P+PE+N+GDVKIVVG N D+IVLDESKD LLEIYAPWCGHCQ LEPTYN
Sbjct: 421 EEKLTPFYKSEPVPESNEGDVKIVVGKNLDQIVLDESKDALLEIYAPWCGHCQELEPTYN 480

Query: 481 KLAKHLHGVDSLVIAKMDGTTNEHPRAKSDGFPTILFFPAGNKSFDPITVDTDRTVVAFY 540
           KL KHL G+DSLVIAKMDGT NEHPRAK DGFPTILF+PAG KSF+PIT + DRTVV  Y
Sbjct: 481 KLGKHLRGIDSLVIAKMDGTANEHPRAKPDGFPTILFYPAGKKSFEPITFEGDRTVVEMY 540

Query: 541 KFLKKNASIPFKLQKPVSS-PKAESSEGKSSDDAK-ESPKSTDLKDEL 584
           KF+KK+ASIPFKL++P SS  K E  +  +S + + E    T+ KDEL
Sbjct: 541 KFIKKHASIPFKLKRPDSSATKTEKDQSTASTNLRGERSSGTNFKDEL 563

BLAST of Cucsa.226930 vs. Swiss-Prot
Match: PDIA1_CHICK (Protein disulfide-isomerase OS=Gallus gallus GN=P4HB PE=1 SV=3)

HSP 1 Score: 332.0 bits (850), Expect = 1.3e-89
Identity = 184/459 (40.09%), Postives = 275/459 (59.91%), Query Frame = 1

Query: 93  PVVDEKDVVVLKEGNFSDFIKKNRFVMVEFYAPWCGHCQALAPEYAAAATELKAEN--VA 152
           P+ +E  V+VL+  NF   +  +R ++VEFYAPWCGHC+ALAPEYA AA +LKAE   + 
Sbjct: 24  PLEEEDGVLVLRAANFEQALAAHRHLLVEFYAPWCGHCKALAPEYAKAAAQLKAEGSEIR 83

Query: 153 LAKVDATEENELAQQYDVQGFPTVYFFSDGVHKA---YPGQRTKDAIVSWIKKKTGPGIY 212
           LAKVDATEE ELAQQ+ V+G+PT+ FF +G   A   Y   R  D IVSW+KK+TGP   
Sbjct: 84  LAKVDATEEAELAQQFGVRGYPTIKFFRNGDKAAPREYTAGREADDIVSWLKKRTGPAAT 143

Query: 213 NITSVEDAERILTSESKVAVGYLNSLVGSESDELAAASRLEDDVNFYQTVDPEVAKLFHI 272
            +T    AE ++ S   V +G+   +    + E   A+   DD+ F  +   +V   + +
Sbjct: 144 TLTDAAAAETLVDSSEVVVIGFFKDVTSDAAKEFLLAAESVDDIPFGISSSADVFSKYQL 203

Query: 273 EASAKRPALVLLKKEAEKLSRFDGEFSKSAIVEFVFANKLPLVTMFTKENAPLIFESSIK 332
                +  +VL KK  E  + F+G+ +K  ++ F+ +N+LPLV  FT++ AP IF   IK
Sbjct: 204 S----QDGVVLFKKFDEGRNNFEGDLTKDNLLNFIKSNQLPLVIEFTEQTAPKIFGGEIK 263

Query: 333 KQLILFAISN--DTEKLIPIFEEAAKSFKGKLIFVYVEIDNEEVGKPVSEYFGVNGNG-P 392
             ++LF   +  D E  +  F+ AA +FKGK++F++++ D+ +  + + E+FG+     P
Sbjct: 264 THILLFLPKSVSDYEGKLDNFKTAAGNFKGKILFIFIDSDHSD-NQRILEFFGLKKEECP 323

Query: 393 EVLGYTGNEDSKKFVLDKE-VTLENIKAFAENFLEDKLKPFYKSDPIPETNDGD-VKIVV 452
            V   T  E+  K+  + + +T + IK F   FLE K+KP   S  +PE  D   VK++V
Sbjct: 324 AVRLITLEEEMTKYKPESDDLTADKIKEFCNKFLEGKIKPHLMSQDLPEDWDKQPVKVLV 383

Query: 453 GDNFDEIVLDESKDVLLEIYAPWCGHCQALEPTYNKLAKHLHGVDSLVIAKMDGTTNEHP 512
           G NF+E+  DE+K+V +E YAPWCGHC+ L P ++KL +     +++VIAKMD T NE  
Sbjct: 384 GKNFEEVAFDENKNVFVEFYAPWCGHCKQLAPIWDKLGETYRDHENIVIAKMDSTANEVE 443

Query: 513 RAKSDGFPTILFFPAGNKSFDPITVDTDRTVVAFYKFLK 542
             K   FPT+ FFPAG+   + I  + +RT+  F KFL+
Sbjct: 444 AVKIHSFPTLKFFPAGS-GRNVIDYNGERTLEGFKKFLE 476


HSP 2 Score: 68.6 bits (166), Expect = 2.7e-10
Identity = 45/126 (35.71%), Postives = 67/126 (53.17%), Query Frame = 1

Query: 96  DEKDVVVLKEGNFSDF-IKKNRFVMVEFYAPWCGHCQALAPEY-AAAATELKAENVALAK 155
           D++ V VL   NF +    +N+ V VEFYAPWCGHC+ LAP +     T    EN+ +AK
Sbjct: 370 DKQPVKVLVGKNFEEVAFDENKNVFVEFYAPWCGHCKQLAPIWDKLGETYRDHENIVIAK 429

Query: 156 VDATEENELAQQYDVQGFPTVYFFSDGVHK---AYPGQRTKDAIVSWIKKKTGPGIYNIT 215
           +D+T  NE+ +   +  FPT+ FF  G  +    Y G+RT +    +++     G     
Sbjct: 430 MDST-ANEV-EAVKIHSFPTLKFFPAGSGRNVIDYNGERTLEGFKKFLESGGQDGAAADD 489

Query: 216 SVEDAE 217
            +ED E
Sbjct: 490 DLEDLE 493

BLAST of Cucsa.226930 vs. Swiss-Prot
Match: PDIA1_BOVIN (Protein disulfide-isomerase OS=Bos taurus GN=P4HB PE=1 SV=1)

HSP 1 Score: 332.0 bits (850), Expect = 1.3e-89
Identity = 193/501 (38.52%), Postives = 290/501 (57.88%), Query Frame = 1

Query: 96  DEKD-VVVLKEGNFSDFIKKNRFVMVEFYAPWCGHCQALAPEYAAAATELKAEN--VALA 155
           DE+D V+VL +GNF + +  +++++VEFYAPWCGHC+ALAPEYA AA +LKAE   + LA
Sbjct: 23  DEEDHVLVLHKGNFDEALAAHKYLLVEFYAPWCGHCKALAPEYAKAAGKLKAEGSEIRLA 82

Query: 156 KVDATEENELAQQYDVQGFPTVYFFSDG---VHKAYPGQRTKDAIVSWIKKKTGPGIYNI 215
           KVDATEE++LAQQY V+G+PT+ FF +G     K Y   R  D IV+W+KK+TGP    +
Sbjct: 83  KVDATEESDLAQQYGVRGYPTIKFFKNGDTASPKEYTAGREADDIVNWLKKRTGPAASTL 142

Query: 216 TSVEDAERILTSESKVAVGYLNSLVGSESDELAAASRLEDDVNFYQTVDPEVAKLFHIEA 275
           +    AE ++ S     +G+   +    + +   A+ + DD+ F  T + +V   + ++ 
Sbjct: 143 SDGAAAEALVESSEVAVIGFFKDMESDSAKQFFLAAEVIDDIPFGITSNSDVFSKYQLD- 202

Query: 276 SAKRPALVLLKKEAEKLSRFDGEFSKSAIVEFVFANKLPLVTMFTKENAPLIFESSIKKQ 335
              +  +VL KK  E  + F+GE +K  +++F+  N+LPLV  FT++ AP IF   IK  
Sbjct: 203 ---KDGVVLFKKFDEGRNNFEGEVTKEKLLDFIKHNQLPLVIEFTEQTAPKIFGGEIKTH 262

Query: 336 LILFAISN--DTEKLIPIFEEAAKSFKGKLIFVYVEIDNEEVGKPVSEYFGVNGNG-PEV 395
           ++LF   +  D E  +  F++AA+SFKGK++F++++ D+ +  + + E+FG+     P V
Sbjct: 263 ILLFLPKSVSDYEGKLSNFKKAAESFKGKILFIFIDSDHTD-NQRILEFFGLKKEECPAV 322

Query: 396 LGYTGNEDSKKFVLDK-EVTLENIKAFAENFLEDKLKPFYKSDPIPETNDGD-VKIVVGD 455
              T  E+  K+  +  E+T E I  F   FLE K+KP   S  +P+  D   VK++VG 
Sbjct: 323 RLITLEEEMTKYKPESDELTAEKITEFCHRFLEGKIKPHLMSQELPDDWDKQPVKVLVGK 382

Query: 456 NFDEIVLDESKDVLLEIYAPWCGHCQALEPTYNKLAKHLHGVDSLVIAKMDGTTNEHPRA 515
           NF+E+  DE K+V +E YAPWCGHC+ L P ++KL +     +++VIAKMD T NE    
Sbjct: 383 NFEEVAFDEKKNVFVEFYAPWCGHCKQLAPIWDKLGETYKDHENIVIAKMDSTANEVEAV 442

Query: 516 KSDGFPTILFFPAGNKSFDPITVD--TDRTVVAFYKFLKKNASIPFKLQKPVSSPKAESS 575
           K   FPT+ FFPA   S D   +D   +RT+  F KFL+                  E  
Sbjct: 443 KVHSFPTLKFFPA---SADRTVIDYNGERTLDGFKKFLESGGQ-----DGAGDDDDLEDL 502

Query: 576 EGKSSDDAKESPKSTDLKDEL 584
           E     D +E      +KDEL
Sbjct: 503 EEAEEPDLEEDDDQKAVKDEL 510

BLAST of Cucsa.226930 vs. TrEMBL
Match: A0A0A0LUQ2_CUCSA (Protein disulfide-isomerase OS=Cucumis sativus GN=Csa_1G084280 PE=3 SV=1)

HSP 1 Score: 1165.2 bits (3013), Expect = 0.0e+00
Identity = 583/583 (100.00%), Postives = 583/583 (100.00%), Query Frame = 1

Query: 1   MATRTLLLLAMAALLLFSNSVLCKDDSPPKDPLSKPTDDDDEDLSFLEEPDEAHAHQNLP 60
           MATRTLLLLAMAALLLFSNSVLCKDDSPPKDPLSKPTDDDDEDLSFLEEPDEAHAHQNLP
Sbjct: 1   MATRTLLLLAMAALLLFSNSVLCKDDSPPKDPLSKPTDDDDEDLSFLEEPDEAHAHQNLP 60

Query: 61  DFDNFEGGAEDEDFGDFTDFEDAEGDGDEYKAPVVDEKDVVVLKEGNFSDFIKKNRFVMV 120
           DFDNFEGGAEDEDFGDFTDFEDAEGDGDEYKAPVVDEKDVVVLKEGNFSDFIKKNRFVMV
Sbjct: 61  DFDNFEGGAEDEDFGDFTDFEDAEGDGDEYKAPVVDEKDVVVLKEGNFSDFIKKNRFVMV 120

Query: 121 EFYAPWCGHCQALAPEYAAAATELKAENVALAKVDATEENELAQQYDVQGFPTVYFFSDG 180
           EFYAPWCGHCQALAPEYAAAATELKAENVALAKVDATEENELAQQYDVQGFPTVYFFSDG
Sbjct: 121 EFYAPWCGHCQALAPEYAAAATELKAENVALAKVDATEENELAQQYDVQGFPTVYFFSDG 180

Query: 181 VHKAYPGQRTKDAIVSWIKKKTGPGIYNITSVEDAERILTSESKVAVGYLNSLVGSESDE 240
           VHKAYPGQRTKDAIVSWIKKKTGPGIYNITSVEDAERILTSESKVAVGYLNSLVGSESDE
Sbjct: 181 VHKAYPGQRTKDAIVSWIKKKTGPGIYNITSVEDAERILTSESKVAVGYLNSLVGSESDE 240

Query: 241 LAAASRLEDDVNFYQTVDPEVAKLFHIEASAKRPALVLLKKEAEKLSRFDGEFSKSAIVE 300
           LAAASRLEDDVNFYQTVDPEVAKLFHIEASAKRPALVLLKKEAEKLSRFDGEFSKSAIVE
Sbjct: 241 LAAASRLEDDVNFYQTVDPEVAKLFHIEASAKRPALVLLKKEAEKLSRFDGEFSKSAIVE 300

Query: 301 FVFANKLPLVTMFTKENAPLIFESSIKKQLILFAISNDTEKLIPIFEEAAKSFKGKLIFV 360
           FVFANKLPLVTMFTKENAPLIFESSIKKQLILFAISNDTEKLIPIFEEAAKSFKGKLIFV
Sbjct: 301 FVFANKLPLVTMFTKENAPLIFESSIKKQLILFAISNDTEKLIPIFEEAAKSFKGKLIFV 360

Query: 361 YVEIDNEEVGKPVSEYFGVNGNGPEVLGYTGNEDSKKFVLDKEVTLENIKAFAENFLEDK 420
           YVEIDNEEVGKPVSEYFGVNGNGPEVLGYTGNEDSKKFVLDKEVTLENIKAFAENFLEDK
Sbjct: 361 YVEIDNEEVGKPVSEYFGVNGNGPEVLGYTGNEDSKKFVLDKEVTLENIKAFAENFLEDK 420

Query: 421 LKPFYKSDPIPETNDGDVKIVVGDNFDEIVLDESKDVLLEIYAPWCGHCQALEPTYNKLA 480
           LKPFYKSDPIPETNDGDVKIVVGDNFDEIVLDESKDVLLEIYAPWCGHCQALEPTYNKLA
Sbjct: 421 LKPFYKSDPIPETNDGDVKIVVGDNFDEIVLDESKDVLLEIYAPWCGHCQALEPTYNKLA 480

Query: 481 KHLHGVDSLVIAKMDGTTNEHPRAKSDGFPTILFFPAGNKSFDPITVDTDRTVVAFYKFL 540
           KHLHGVDSLVIAKMDGTTNEHPRAKSDGFPTILFFPAGNKSFDPITVDTDRTVVAFYKFL
Sbjct: 481 KHLHGVDSLVIAKMDGTTNEHPRAKSDGFPTILFFPAGNKSFDPITVDTDRTVVAFYKFL 540

Query: 541 KKNASIPFKLQKPVSSPKAESSEGKSSDDAKESPKSTDLKDEL 584
           KKNASIPFKLQKPVSSPKAESSEGKSSDDAKESPKSTDLKDEL
Sbjct: 541 KKNASIPFKLQKPVSSPKAESSEGKSSDDAKESPKSTDLKDEL 583

BLAST of Cucsa.226930 vs. TrEMBL
Match: W9RV77_9ROSA (Protein disulfide-isomerase OS=Morus notabilis GN=L484_007658 PE=3 SV=1)

HSP 1 Score: 892.5 bits (2305), Expect = 2.8e-256
Identity = 441/590 (74.75%), Postives = 507/590 (85.93%), Query Frame = 1

Query: 1   MATRTLLLLAMAALLLFSNSVLCKDDSPPKDPLSKPTDDDDEDLSFLEEPDEAH-----A 60
           MA+R  + LA AALL FSNSVLCK+        +K  DDDDEDLSFLEE D+        
Sbjct: 1   MASRLSIALAFAALLFFSNSVLCKESK------AKDLDDDDEDLSFLEESDDKSDANHGG 60

Query: 61  HQNLPDFDNFEGGAEDEDFGDFTDFEDAEGDGDEYKAPVVDEKDVVVLKEGNFSDFIKKN 120
           H + PD DNF+    D+DFG+F+DF++ + D + YK P VD+KDVVVLKE NF+  I+ N
Sbjct: 61  HNHYPDPDNFDDEG-DDDFGNFSDFDEPDADQEAYKEPEVDDKDVVVLKERNFTGVIENN 120

Query: 121 RFVMVEFYAPWCGHCQALAPEYAAAATELKAENVALAKVDATEENELAQQYDVQGFPTVY 180
           +FVMVEFYAPWCGHCQALAPEYAAAATELK ENV LAKVDATEENELA +YDVQG+PT+Y
Sbjct: 121 QFVMVEFYAPWCGHCQALAPEYAAAATELKGENVVLAKVDATEENELAHEYDVQGYPTIY 180

Query: 181 FFSDGVHKAYPGQRTKDAIVSWIKKKTGPGIYNITSVEDAERILTSESKVAVGYLNSLVG 240
           FF DGVHK Y G R K+AIV+WIKKK GPGI NIT+ EDAERILTSESKV + YLNSLVG
Sbjct: 181 FFVDGVHKPYSGARNKEAIVTWIKKKIGPGINNITTTEDAERILTSESKVVLAYLNSLVG 240

Query: 241 SESDELAAASRLEDDVNFYQTVDPEVAKLFHIEASAKRPALVLLKKEAEKLSRFDGEFSK 300
           SESD LA+ASRLEDDVNFYQTV+P+VAKLFHIE   KRPALVLLK+E EKL  FDG+F K
Sbjct: 241 SESDVLASASRLEDDVNFYQTVNPDVAKLFHIEPEVKRPALVLLKEEPEKLIHFDGKFDK 300

Query: 301 SAIVEFVFANKLPLVTMFTKENAPLIFESSIKKQLILFAISNDTEKLIPIFEEAAKSFKG 360
           S+I EFVFANKLPLVT+FT+ENAP +FES+IKKQL+LF+  NDTEK++PIF+EAAKSFKG
Sbjct: 301 SSISEFVFANKLPLVTIFTRENAPAVFESTIKKQLLLFSTKNDTEKVVPIFQEAAKSFKG 360

Query: 361 KLIFVYVEIDNEEVGKPVSEYFGVNGNGPEVLGYTGNEDSKKFVLDKEVTLENIKAFAEN 420
           KLIFVY+E+DNE+VGKPVSEYFGV+G+ P+V+ YTGN+D KKFVLD EVT++NIKAF E+
Sbjct: 361 KLIFVYIEMDNEDVGKPVSEYFGVSGDAPKVIAYTGNDDGKKFVLDGEVTVDNIKAFGED 420

Query: 421 FLEDKLKPFYKSDPIPETNDGDVKIVVGDNFDEIVLDESKDVLLEIYAPWCGHCQALEPT 480
           FL+DKLKPF KSDPIPETNDGDVKI+VG+NFDEIVLDESKDVLLEIYAPWCGHCQALEPT
Sbjct: 421 FLQDKLKPFLKSDPIPETNDGDVKIIVGNNFDEIVLDESKDVLLEIYAPWCGHCQALEPT 480

Query: 481 YNKLAKHLHGVDSLVIAKMDGTTNEHPRAKSDGFPTILFFPAGNKSFDPITVDTDRTVVA 540
           YNKLAKHL G++S+VIAKMDGTTNEHPRAKSDGFPTILFFPAGNKSFDPITVDTDRTVVA
Sbjct: 481 YNKLAKHLRGIESIVIAKMDGTTNEHPRAKSDGFPTILFFPAGNKSFDPITVDTDRTVVA 540

Query: 541 FYKFLKKNASIPFKLQKPVSSPKAESSEGKSSDDAKESPKST--DLKDEL 584
           FYKFLKK+ASIPFKLQKP +SPK+ES + K + D  +S +++  DLKDEL
Sbjct: 541 FYKFLKKHASIPFKLQKPTTSPKSESPDAKETSDGTQSTETSTKDLKDEL 583

BLAST of Cucsa.226930 vs. TrEMBL
Match: A0A059D5F7_EUCGR (Protein disulfide-isomerase OS=Eucalyptus grandis GN=EUGRSUZ_B02496 PE=3 SV=1)

HSP 1 Score: 874.4 bits (2258), Expect = 7.8e-251
Identity = 440/596 (73.83%), Postives = 508/596 (85.23%), Query Frame = 1

Query: 1   MATRTLLLL-AMAALLLFSNSVLCKDDSPPKDPLSKPTD-DDDEDLSFLEEPDEAH---- 60
           M+TR LLLL ++AALLL +        S P+   +   D DDDEDLSFLEEP+E H    
Sbjct: 1   MSTRVLLLLLSLAALLLVA--------SFPRSLSAAAVDVDDDEDLSFLEEPEEGHGGAG 60

Query: 61  ------AHQNLPDFDNFEGGAEDEDFGDFTDFEDAEGDGDEYKAPVVDEKDVVVLKEGNF 120
                 AH   PD D+FE   +DEDF +++D +++E D D YK P VD+KDVVVLKEGNF
Sbjct: 61  GAAAAGAHDEFPDSDHFE---DDEDFENYSDLDESEADADAYKEPEVDDKDVVVLKEGNF 120

Query: 121 SDFIKKNRFVMVEFYAPWCGHCQALAPEYAAAATELKAENVALAKVDATEENELAQQYDV 180
           SDF++KNRFVMVEFYAPWCGHCQALAPEYAAAATELK E V LAKVDATEE ELAQ+Y+V
Sbjct: 121 SDFVEKNRFVMVEFYAPWCGHCQALAPEYAAAATELKGEEVVLAKVDATEEEELAQKYEV 180

Query: 181 QGFPTVYFFSDGVHKAYPGQRTKDAIVSWIKKKTGPGIYNITSVEDAERILTSESKVAVG 240
           QGFPTVYFF DGVHK Y GQRTKDAIV+WIKKKTGPG+ N+T+ ++AER+LTSE+KV +G
Sbjct: 181 QGFPTVYFFVDGVHKPYSGQRTKDAIVTWIKKKTGPGVNNLTTADEAERVLTSENKVVLG 240

Query: 241 YLNSLVGSESDELAAASRLEDDVNFYQTVDPEVAKLFHIEASAKRPALVLLKKEAEKLSR 300
           YLN L G ES+ELAAASR+EDDVNFYQTV+P+VAKLFHI+   KRPALV+LKKEAEKLS 
Sbjct: 241 YLNHLKGPESEELAAASRIEDDVNFYQTVNPDVAKLFHIDPEVKRPALVILKKEAEKLSL 300

Query: 301 FDGEFSKSAIVEFVFANKLPLVTMFTKENAPLIFESSIKKQLILFAISNDTEKLIPIFEE 360
           FDG+F KSAI EFVFANKLPLVT FT+E+APLIFES +KKQ++LFA S D+EK  P+FEE
Sbjct: 301 FDGKFEKSAIAEFVFANKLPLVTTFTRESAPLIFESPVKKQVLLFATSKDSEKFFPVFEE 360

Query: 361 AAKSFKGKLIFVYVEIDNEEVGKPVSEYFGVNGNGPEVLGYTGNEDSKKFVLDKEVTLEN 420
           AAK FKGKLIFVYVE+DNE+VGKPVS+YFGV+G+GP+VLGYTGN+DS+KF LD EVTL+N
Sbjct: 361 AAKLFKGKLIFVYVEMDNEDVGKPVSDYFGVSGSGPKVLGYTGNDDSRKFFLDGEVTLDN 420

Query: 421 IKAFAENFLEDKLKPFYKSDPIPETNDGDVKIVVGDNFDEIVLDESKDVLLEIYAPWCGH 480
           IKAFA +FLE+KLKPFYKSDPIPETNDGDVK+VVG+NFDEIVLDESKDVLLEI+APWCGH
Sbjct: 421 IKAFALDFLEEKLKPFYKSDPIPETNDGDVKVVVGNNFDEIVLDESKDVLLEIHAPWCGH 480

Query: 481 CQALEPTYNKLAKHLHGVDSLVIAKMDGTTNEHPRAKSDGFPTILFFPAGNKSFDPITVD 540
           CQALEPTY+KLAKHL G+DSLVIAKMDGTTNEHPRAK+DGFPT+LFFPAGNKSFDPI+VD
Sbjct: 481 CQALEPTYDKLAKHLRGIDSLVIAKMDGTTNEHPRAKADGFPTLLFFPAGNKSFDPISVD 540

Query: 541 TDRTVVAFYKFLKKNASIPFKLQKP-VSSPKAESSEGKSSDDAKESPKSTDLKDEL 584
           +DRTVVA YKFLKKNASIPFKLQKP  S+PK  +++ K S    E   +TDLKDEL
Sbjct: 541 SDRTVVALYKFLKKNASIPFKLQKPAASTPKPATTDTKES----EETVTTDLKDEL 581

BLAST of Cucsa.226930 vs. TrEMBL
Match: B9HQ95_POPTR (Protein disulfide-isomerase OS=Populus trichocarpa GN=POPTR_0009s01920g PE=3 SV=1)

HSP 1 Score: 864.4 bits (2232), Expect = 8.0e-248
Identity = 432/595 (72.61%), Postives = 508/595 (85.38%), Query Frame = 1

Query: 1   MATRTLLLLAMAALLLFSNSVLCKDDSPPKDPLSKPT-DDDDEDLSFLEEPDEA------ 60
           M+TR + LL++ ALLLFS+       SP    L     +DDDEDLSFLEE  +A      
Sbjct: 1   MSTRFIFLLSLTALLLFSHL------SPSLSKLQNAAAEDDDEDLSFLEEETDAVPHGQG 60

Query: 61  ----HAHQNLPDFDNFEGGAEDED-FGDFTDFEDAEGDGDEYKAPVVDEKDVVVLKEGNF 120
               H H + PD D F+   ++ED   +++D +D+E D   YK P +D+KDVVVLKEGNF
Sbjct: 61  HGHDHDHDHYPDPDQFDEEFDNEDDLDNYSDLDDSELDS--YKEPEIDDKDVVVLKEGNF 120

Query: 121 SDFIKKNRFVMVEFYAPWCGHCQALAPEYAAAATELKAENVALAKVDATEENELAQQYDV 180
           SDF+ KN+FVMVEFYAPWCGHCQ+LAPEYAAAATELKAE V LAKVDATEENELAQ+YD+
Sbjct: 121 SDFVTKNKFVMVEFYAPWCGHCQSLAPEYAAAATELKAEEVMLAKVDATEENELAQEYDI 180

Query: 181 QGFPTVYFFSDGVHKAYPGQRTKDAIVSWIKKKTGPGIYNITSVEDAERILTSESKVAVG 240
           QGFPTVYFF DGVH+ YPG R KD IV+WIKKK GPGIYNIT+V+DAER+LTSE+K+ +G
Sbjct: 181 QGFPTVYFFVDGVHRPYPGPRNKDGIVTWIKKKIGPGIYNITTVDDAERLLTSETKLVLG 240

Query: 241 YLNSLVGSESDELAAASRLEDDVNFYQTVDPEVAKLFHIEASAKRPALVLLKKEAEKLSR 300
           +LNSLVG ES+ELAAASRLED+V+FYQTV+P+VAKLFH++  AKRPALV+LKKEAEKLS 
Sbjct: 241 FLNSLVGPESEELAAASRLEDEVSFYQTVNPDVAKLFHLDPQAKRPALVMLKKEAEKLSV 300

Query: 301 FDGEFSKSAIVEFVFANKLPLVTMFTKENAPLIFESSIKKQLILFAISNDTEKLIPIFEE 360
           FDG FSKS I EFVFANKLPLVT+FT+E+APLIFES+IKKQL+LFAISND+EK++PIF+E
Sbjct: 301 FDGNFSKSEIAEFVFANKLPLVTIFTRESAPLIFESTIKKQLLLFAISNDSEKVVPIFQE 360

Query: 361 AAKSFKGKLIFVYVEIDNEEVGKPVSEYFGVNGNGPEVLGYTGNEDSKKFVLDKEVTLEN 420
           AA+ FKGKLIFVYVE+DNE+VGKPVSEYFG++G  P+VL YTGN+D+KKFV D +VTL+ 
Sbjct: 361 AARLFKGKLIFVYVEMDNEDVGKPVSEYFGISGTAPKVLAYTGNDDAKKFVFDGDVTLDK 420

Query: 421 IKAFAENFLEDKLKPFYKSDPIPETNDGDVKIVVGDNFDEIVLDESKDVLLEIYAPWCGH 480
           IKAF E+F+EDKLKPF+KSDP+PE+NDGDVKIVVG+NFDEIVLDESKDVLLEIYAPWCGH
Sbjct: 421 IKAFGEDFIEDKLKPFFKSDPVPESNDGDVKIVVGNNFDEIVLDESKDVLLEIYAPWCGH 480

Query: 481 CQALEPTYNKLAKHLHGVDSLVIAKMDGTTNEHPRAKSDGFPTILFFPAGNKSFDPITVD 540
           CQ+LEPTYNKLA HL G++S+VIAKMDGTTNEHPRAKSDGFPT+LFFPAGNKSFDPITVD
Sbjct: 481 CQSLEPTYNKLATHLRGIESIVIAKMDGTTNEHPRAKSDGFPTLLFFPAGNKSFDPITVD 540

Query: 541 TDRTVVAFYKFLKKNASIPFKLQKPVSSPKAESSEGKSSDDAKESPKSTDLKDEL 584
           TDRTVVAFYKF+KK+ASIPFKLQKP S+ KAESS+ K   D  ES  + D+KDEL
Sbjct: 541 TDRTVVAFYKFIKKHASIPFKLQKPASASKAESSDAK---DGIES-STRDVKDEL 583

BLAST of Cucsa.226930 vs. TrEMBL
Match: M5W6Y1_PRUPE (Protein disulfide-isomerase OS=Prunus persica GN=PRUPE_ppa003432mg PE=3 SV=1)

HSP 1 Score: 862.1 bits (2226), Expect = 4.0e-247
Identity = 429/586 (73.21%), Postives = 500/586 (85.32%), Query Frame = 1

Query: 1   MATRTLLLLAMAALLLFSNSVLCKDDSPPKDPLSKPTDDDDEDLSFLEEP-DEAHAHQNL 60
           MA+R +L+ A++ALL FS S LCKD        S P DDDD+DLSFLEEP D+  A  + 
Sbjct: 1   MASRFVLVFALSALLFFSRSALCKD--------SNPIDDDDDDLSFLEEPTDQNDAAPHY 60

Query: 61  PDFDNFEGGAEDEDFGDFTDFEDAEGDG-DEYKAPVVDEKDVVVLKEGNFSDFIKKNRFV 120
           PD D+++    D D  +F+DF++  GD  D YK P VDEKDVVVLK  NFSD ++KNRFV
Sbjct: 61  PDPDHYDEDNYD-DLENFSDFDEGGGDHEDAYKEPEVDEKDVVVLKGTNFSDTVEKNRFV 120

Query: 121 MVEFYAPWCGHCQALAPEYAAAATELKAENVALAKVDATEENELAQQYDVQGFPTVYFFS 180
           MVEFYAPWCGHCQALAPEYAAAATELK ENV LAK+D+TEENEL+Q+Y V+GFPTV+FF 
Sbjct: 121 MVEFYAPWCGHCQALAPEYAAAATELKGENVILAKIDSTEENELSQEYGVEGFPTVFFFI 180

Query: 181 DGVHKAYPGQRTKDAIVSWIKKKTGPGIYNITSVEDAERILTSESKVAVGYLNSLVGSES 240
           DGVHK Y GQRTK+ IV+WIKKK GPGI N+T++++AERILTSESKV +GYLNSLVG ES
Sbjct: 181 DGVHKPYTGQRTKEGIVTWIKKKIGPGIQNVTTLDEAERILTSESKVVLGYLNSLVGPES 240

Query: 241 DELAAASRLEDDVNFYQTVDPEVAKLFHIEASAKRPALVLLKKEAEKLSRFDGEFSKSAI 300
           DELAAASRLEDDVNFYQTVDP+VAKLFH++   KRPAL+LLKKEAEKLS FDG+F KSAI
Sbjct: 241 DELAAASRLEDDVNFYQTVDPKVAKLFHLDPEVKRPALILLKKEAEKLSYFDGKFEKSAI 300

Query: 301 VEFVFANKLPLVTMFTKENAPLIFESSIKKQLILFAISNDTEKLIPIFEEAAKSFKGKLI 360
            EFVFANKLPLV  FT+ENAP IFES IKKQL+LFA S D+EK++P F+ AAK FKGKLI
Sbjct: 301 AEFVFANKLPLVITFTRENAPQIFESEIKKQLLLFATSKDSEKVLPDFQAAAKLFKGKLI 360

Query: 361 FVYVEIDNEEVGKPVSEYFGVNGNGPEVLGYTGNEDSKKFVLDKEVTLENIKAFAENFLE 420
           FVYV+ DNE++GKPVS+YFG+  + P VLGYTGN+DS+KFV + EVTL N+KAF ++F+E
Sbjct: 361 FVYVQTDNEDIGKPVSDYFGITSDAPTVLGYTGNDDSRKFVHEGEVTLANLKAFGQDFIE 420

Query: 421 DKLKPFYKSDPIPETNDGDVKIVVGDNFDEIVLDESKDVLLEIYAPWCGHCQALEPTYNK 480
           DKLKPF+KSDPIPETNDGDVKIVVG+NFDEIVLDESKDVLLEIYAPWCGHCQ+LEPTYNK
Sbjct: 421 DKLKPFFKSDPIPETNDGDVKIVVGNNFDEIVLDESKDVLLEIYAPWCGHCQSLEPTYNK 480

Query: 481 LAKHLHGVDSLVIAKMDGTTNEHPRAKSDGFPTILFFPAGNKSFDPITVDTDRTVVAFYK 540
           LAKHL G+DS+VIAKMDGTTNEHPRAK+DGFPT+LFFPAGNKSFDPI+VD DRTVVAFYK
Sbjct: 481 LAKHLRGIDSIVIAKMDGTTNEHPRAKADGFPTLLFFPAGNKSFDPISVDADRTVVAFYK 540

Query: 541 FLKKNASIPFKLQKPVSSPKAES-SEGKSSDDAKESPKSTDLKDEL 584
           FLKKNASIPFKLQKP S+PK+E+ S+ K S ++  S  + DL+DEL
Sbjct: 541 FLKKNASIPFKLQKPTSTPKSEAVSDAKESQESSSS--TNDLRDEL 575

BLAST of Cucsa.226930 vs. TAIR10
Match: AT5G60640.1 (AT5G60640.1 PDI-like 1-4)

HSP 1 Score: 776.2 bits (2003), Expect = 1.5e-224
Identity = 389/605 (64.30%), Postives = 472/605 (78.02%), Query Frame = 1

Query: 1   MATRTLLLLAMAALLLFSNSVLCKDDSPPKDPLSKPTDDDDEDLSFLEEPDE-----AHA 60
           MA R LLL ++ ALL+FS          P    S   D DDEDLSFLE+  E     A +
Sbjct: 1   MAFRVLLLFSLTALLIFSAV-------SPSFAASSSDDVDDEDLSFLEDLKEDDVPGADS 60

Query: 61  HQNLPDFDNFEGGAEDEDFGDFTDFEDAEGD--------GDEYKAPVVDEKDVVVLKEGN 120
             +   FD FEGG E+ED   + D +D EGD         D    P +DEKDVVV+KE N
Sbjct: 61  LSSSTGFDEFEGG-EEEDPDMYNDDDDEEGDFSDLGNPDSDPLPTPEIDEKDVVVIKERN 120

Query: 121 FSDFIKKNRFVMVEFYAPWCGHCQALAPEYAAAATELKAENVALAKVDATEENELAQQYD 180
           F+D I+ N++V+VEFYAPWCGHCQ+LAPEYAAAATELK + V LAK+DATEENELAQ+Y 
Sbjct: 121 FTDVIENNQYVLVEFYAPWCGHCQSLAPEYAAAATELKEDGVVLAKIDATEENELAQEYR 180

Query: 181 VQGFPTVYFFSDGVHKAYPGQRTKDAIVSWIKKKTGPGIYNITSVEDAERILTSESKVAV 240
           VQGFPT+ FF DG HK Y G RTK+ IV+W+KKK GPG+YN+T+++DAE++LTS +KV +
Sbjct: 181 VQGFPTLLFFVDGEHKPYTGGRTKETIVTWVKKKIGPGVYNLTTLDDAEKVLTSGNKVVL 240

Query: 241 GYLNSLVGSESDELAAASRLEDDVNFYQTVDPEVAKLFHIEASAKRPALVLLKKEAEKLS 300
           GYLNSLVG E D+L AAS+ EDDVNFYQTV+P+VAK+FH++  +KRPALVL+KKE EK+S
Sbjct: 241 GYLNSLVGVEHDQLNAASKAEDDVNFYQTVNPDVAKMFHLDPESKRPALVLVKKEEEKIS 300

Query: 301 RFDGEFSKSAIVEFVFANKLPLVTMFTKENAPLIFESSIKKQLILFAISNDTEKLIPIFE 360
            FDGEF KSA+V FV ANKL LV++FT+E AP IFES+IKKQL+LF   N++EK++  F+
Sbjct: 301 HFDGEFVKSALVSFVSANKLALVSVFTRETAPEIFESAIKKQLLLFVTKNESEKVLTEFQ 360

Query: 361 EAAKSFKGKLIFVYVEIDNEEVGKPVSEYFGVNGNGPEVLGYTGNEDSKKFVLDKEVTLE 420
           EAAKSFKGKLIFV V++DNE+ GKPV+EYFGV+GNGP+++GYTGNED KK+  D E+  +
Sbjct: 361 EAAKSFKGKLIFVSVDLDNEDYGKPVAEYFGVSGNGPKLIGYTGNEDPKKYFFDGEIQSD 420

Query: 421 NIKAFAENFLEDKLKPFYKSDPIPETNDGDVKIVVGDNFDEIVLDESKDVLLEIYAPWCG 480
            IK F E+FL DKLKPFYKSDPIPE ND DVKIVVGDNFDEIVLD+SKDVLLE+YAPWCG
Sbjct: 421 KIKIFGEDFLNDKLKPFYKSDPIPEKNDEDVKIVVGDNFDEIVLDDSKDVLLEVYAPWCG 480

Query: 481 HCQALEPTYNKLAKHLHGVDSLVIAKMDGTTNEHPRAKSDGFPTILFFPAGNKSFDPITV 540
           HCQALEP YNKLAKHL  +DSLVI KMDGTTNEHP+AK++GFPTILFFPAGNK+ +PITV
Sbjct: 481 HCQALEPMYNKLAKHLRSIDSLVITKMDGTTNEHPKAKAEGFPTILFFPAGNKTSEPITV 540

Query: 541 DTDRTVVAFYKFLKKNASIPFKLQKPV---------SSPKAESSEGKSSDDAKESPKSTD 584
           DTDRTVVAFYKFL+K+A+IPFKL+KP          S+PK E++E K S D+      +D
Sbjct: 541 DTDRTVVAFYKFLRKHATIPFKLEKPASTESPKTAESTPKVETTETKESPDSTTKSSQSD 597

BLAST of Cucsa.226930 vs. TAIR10
Match: AT3G54960.1 (AT3G54960.1 PDI-like 1-3)

HSP 1 Score: 698.7 bits (1802), Expect = 3.0e-201
Identity = 357/572 (62.41%), Postives = 435/572 (76.05%), Query Frame = 1

Query: 13  ALLLFSNSVLCKDDSPPKDPLSKPTDDDDEDLSFLEEPDEAHAHQNLPDFDNFEGGAEDE 72
           +LLLF + +L   +S  ++  S    D DE+L+FL   +          +   E   +  
Sbjct: 9   SLLLFVSFILLLVNSRAENASSG--SDLDEELAFLAAEESKEQSHGGGSYHEEEHDHQHR 68

Query: 73  DFGDFTDFEDAEGD---GD----EYKAPVVDEKDVVVLKEGNFSDFIKKNRFVMVEFYAP 132
           DF ++ D E   G+   GD    E   P VDEKDV VL + NF++F+  N F MVEFYAP
Sbjct: 69  DFENYDDLEQGGGEFHHGDHGYEEEPLPPVDEKDVAVLTKDNFTEFVGNNSFAMVEFYAP 128

Query: 133 WCGHCQALAPEYAAAATELKAENVALAKVDATEENELAQQYDVQGFPTVYFFSDG-VHKA 192
           WCG CQAL PEYAAAATELK    ALAK+DATEE +LAQ+Y++QGFPTV+ F DG + K 
Sbjct: 129 WCGACQALTPEYAAAATELKGL-AALAKIDATEEGDLAQKYEIQGFPTVFLFVDGEMRKT 188

Query: 193 YPGQRTKDAIVSWIKKKTGPGIYNITSVEDAERILTSESKVAVGYLNSLVGSESDELAAA 252
           Y G+RTKD IV+W+KKK  P I+NIT+ E+AER+L++E K+  G+LNSLVGSES+ELAAA
Sbjct: 189 YEGERTKDGIVTWLKKKASPSIHNITTKEEAERVLSAEPKLVFGFLNSLVGSESEELAAA 248

Query: 253 SRLEDDVNFYQTVDPEVAKLFHIEASAKRPALVLLKKEAEKLSRFDGEFSKSAIVEFVFA 312
           SRLEDD++FYQT  P++AKLF IE   KRPALVLLKKE EKL+RFDG F+K+AI EFV A
Sbjct: 249 SRLEDDLSFYQTASPDIAKLFEIETQVKRPALVLLKKEEEKLARFDGNFTKTAIAEFVSA 308

Query: 313 NKLPLVTMFTKENAPLIFESSIKKQLILFAISNDTEKLIPIFEEAAKSFKGKLIFVYVEI 372
           NK+PLV  FT+E A LIFESS+K QLILFA +N++EK +P   E AKSFKGK +FVYV++
Sbjct: 309 NKVPLVINFTREGASLIFESSVKNQLILFAKANESEKHLPTLREVAKSFKGKFVFVYVQM 368

Query: 373 DNEEVGKPVSEYFGVNGNGPEVLGYTGNEDSKKFVLDKEVTLENIKAFAENFLEDKLKPF 432
           DNE+ G+ VS +FGV G  P+VL YTGNED +KF+LD E+T+ NIK  AE+FL DKLKPF
Sbjct: 369 DNEDYGEAVSGFFGVTGAAPKVLVYTGNEDMRKFILDGELTVNNIKTLAEDFLADKLKPF 428

Query: 433 YKSDPIPETNDGDVKIVVGDNFDEIVLDESKDVLLEIYAPWCGHCQALEPTYNKLAKHLH 492
           YKSDP+PE NDGDVK++VG+NFDEIVLDESKDVLLEIYAPWCGHCQ+ EP YNKL K+L 
Sbjct: 429 YKSDPLPENNDGDVKVIVGNNFDEIVLDESKDVLLEIYAPWCGHCQSFEPIYNKLGKYLK 488

Query: 493 GVDSLVIAKMDGTTNEHPRAKSDGFPTILFFPAGNKSFDPITVDTDRTVVAFYKFLKKNA 552
           G+DSLV+AKMDGT+NEHPRAK+DGFPTILFFP GNKSFDPI VD DRTVV  YKFLKK+A
Sbjct: 489 GIDSLVVAKMDGTSNEHPRAKADGFPTILFFPGGNKSFDPIAVDVDRTVVELYKFLKKHA 548

Query: 553 SIPFKLQKP------VSSPKA-ESSEGKSSDD 570
           SIPFKL+KP      +S+ K+ E  EG SS D
Sbjct: 549 SIPFKLEKPATPEPVISTMKSDEKIEGDSSKD 577

BLAST of Cucsa.226930 vs. TAIR10
Match: AT1G52260.1 (AT1G52260.1 PDI-like 1-5)

HSP 1 Score: 293.5 bits (750), Expect = 2.9e-79
Identity = 151/444 (34.01%), Postives = 253/444 (56.98%), Query Frame = 1

Query: 91  KAPVVDEKDVVVLK-EGNFSD-FIKKNRFVMVEFYAPWCGHCQALAPEYAAAATELK--A 150
           +A  V +   +VL+  G+++   I  N FVMV  YAPWC     L P +A AAT LK   
Sbjct: 68  EAETVSKAQRIVLELNGDYTKRVIDGNEFVMVLGYAPWCARSAELMPRFAEAATALKEIG 127

Query: 151 ENVALAKVDATEENELAQQYDVQGFPTVYFFSDGVHKAYPGQRTKDAIVSWIKKKTGPGI 210
            +V +AK+D    +++A + +++GFPT+  F +G    Y G  + + IV W++KKTG  I
Sbjct: 128 SSVLMAKIDGDRYSKIASELEIKGFPTLLLFVNGTSLTYNGGSSAEDIVIWVQKKTGAPI 187

Query: 211 YNITSVEDAERILTSESKVAVGYLNSLVGSESDELAAASRLEDDVNFYQTVDPEVAKLFH 270
             + +V++A R L       +G      GSE +E   A++ +D++ F +T D +VAKL  
Sbjct: 188 ITLNTVDEAPRFLDKYHTFVLGLFEKFEGSEHNEFVKAAKSDDEIQFIETRDSDVAKLLF 247

Query: 271 IEASAKRPALVLLKKEAEKLSRFDGEFSKSAIVEFVFANKLPLVTMFTKENAPLIFESSI 330
            +  +    + L+K EAE+ + +DG +    I+EF+ +NK PL T  T+ N   ++ S +
Sbjct: 248 PDLKSNNVFIGLVKPEAERYTVYDGSYKMEKILEFLGSNKFPLFTKLTETNTVWVYSSPV 307

Query: 331 KKQLILFAISNDTEKLIPIFEEAAKSFKGKLIFVYVEIDNEEVGKPVSEYFGVNGNGPEV 390
           K Q++LF+ ++D +KL    E+ A+ FK KL+F+YV+I NE +  P    FG+      V
Sbjct: 308 KLQVMLFSKADDFQKLAQPLEDIARKFKSKLMFIYVDITNENLAMPFLILFGIEAGNKTV 367

Query: 391 LGYTGNEDSKKFVLDKEVTLENIKAFAENFLEDKLKPFYKSDPIPETNDGDVKIVVGDNF 450
           +    N  + K++L+ + +  +I+ F        +  +Y+S+P+P+  +  +  VVG  F
Sbjct: 368 VAAFDNNLNSKYLLESDPSPNSIEEFCSGLAHGTVSRYYRSEPVPDNENASIVTVVGKTF 427

Query: 451 DEIVLDESKDVLLEIYAPWCGHCQALEPTYNKLAKHLHGVDSLVIAKMDGTTNEHPRAK- 510
           D +VL+  ++VLLE++ PWC +C+AL     KLAKH  G ++LV A++D + NEH + + 
Sbjct: 428 DGLVLNSRENVLLEVHTPWCVNCEALSKQIEKLAKHFKGFENLVFARIDASANEHTKLQV 487

Query: 511 SDGFPTILFFPAGNKSFDPITVDT 530
            D +P IL + +G K   P+ + T
Sbjct: 488 DDKYPIILLYKSGEKE-KPLKLST 510

BLAST of Cucsa.226930 vs. TAIR10
Match: AT3G16110.1 (AT3G16110.1 PDI-like 1-6)

HSP 1 Score: 293.1 bits (749), Expect = 3.7e-79
Identity = 153/436 (35.09%), Postives = 244/436 (55.96%), Query Frame = 1

Query: 97  EKDVVVLKEGNFSDFIKKNRFVMVEFYAPWCGHCQALAPEYAAAATELK--AENVALAKV 156
           ++ VV L   N    I  N +VMV  YAPWC     L P +A AAT+LK    +V +AK+
Sbjct: 74  QRIVVELNGDNTKRLIDGNEYVMVLGYAPWCARSAELMPRFAEAATDLKEIGSSVLMAKI 133

Query: 157 DATEENELAQQYDVQGFPTVYFFSDGVHKAYPGQRTKDAIVSWIKKKTGPGIYNITSVED 216
           D    +++A Q +++GFPT+  F +G  ++Y G  + + IV W++KKTG     + +V++
Sbjct: 134 DGERYSKVASQLEIKGFPTLLLFVNGTSQSYTGGFSSEEIVIWVQKKTGASTIKLDTVDE 193

Query: 217 AERILTSESKVAVG-YLNSLVGSESDELAAASRLEDDVNFYQTVDPEVAKLFHIEASAKR 276
           A   L       +G +  S   S  DE   A+ L++++ F +T   +VAKL         
Sbjct: 194 ASGFLKKHHTFILGLFEKSEDSSGHDEFVKAASLDNEIQFVETSSIDVAKLLFPNLKTNN 253

Query: 277 PALVLLKKEAEKLSRFDGEFSKSAIVEFVFANKLPLVTMFTKENAPLIFESSIKKQLILF 336
             + L+K EAEK + +DG      IVEF+ +NK PLVT  T+ N   ++ S +K Q+++F
Sbjct: 254 VFVGLVKTEAEKYTSYDGPCQAEKIVEFLNSNKFPLVTKLTESNTVRVYSSPVKLQVMVF 313

Query: 337 AISNDTEKLIPIFEEAAKSFKGKLIFVYVEIDNEEVGKPVSEYFGVNGNGPEVLGYTGNE 396
           + ++D E L    E+ A+ FK KL+ +Y++I NE +  P    FG+      V+    N 
Sbjct: 314 SKTDDFESLAQPLEDIARKFKSKLMLIYIDISNENLAMPFLTLFGIEDAKKTVVAAFDNN 373

Query: 397 DSKKFVLDKEVTLENIKAFAENFLEDKLKPFYKSDPIPETNDGDVKIVVGDNFDEIVLDE 456
            + K++L+ + +  NI+ F        +  +YKS PIP+  +  V  VVG  FDE+VL  
Sbjct: 374 LNSKYLLESDPSPSNIEEFCFGLAHGTVSAYYKSQPIPDNQNASVVAVVGRTFDEVVLRS 433

Query: 457 SKDVLLEIYAPWCGHCQALEPTYNKLAKHLHGVDSLVIAKMDGTTNEHPRAKSDGFPTIL 516
           S++VLLE++ PWC +C+AL     KL++H  G ++LV A++D + NEHP+   D +PTIL
Sbjct: 434 SENVLLEVHTPWCINCEALSKQVEKLSQHFKGFENLVFARIDASANEHPKLTVDDYPTIL 493

Query: 517 FFPAGNKSFDPITVDT 530
            +  G K  +P+ + T
Sbjct: 494 LYKTGEKE-NPLKLST 508

BLAST of Cucsa.226930 vs. TAIR10
Match: AT1G21750.1 (AT1G21750.1 PDI-like 1-1)

HSP 1 Score: 276.6 bits (706), Expect = 3.6e-74
Identity = 189/504 (37.50%), Postives = 279/504 (55.36%), Query Frame = 1

Query: 100 VVVLKEGNFSDFIKKNRFVMVEFYAPWCGHCQALAPEYAAAATELKAE--NVALAKVDAT 159
           V+ L   NF+D I K+ F++VEFYAPWCGHC+ LAPEY  AA+ L +    V LAK+DA+
Sbjct: 32  VLTLDHTNFTDTINKHDFIVVEFYAPWCGHCKQLAPEYEKAASALSSNVPPVVLAKIDAS 91

Query: 160 EEN--ELAQQYDVQGFPTVYFFSDG--VHKAYPGQRTKDAIVSWIKKKTGPGIYNITSVE 219
           EE   E A QY+VQGFPT+  F +G    + Y G R  + IV+++KK++GP    I S +
Sbjct: 92  EETNREFATQYEVQGFPTIKIFRNGGKAVQEYNGPREAEGIVTYLKKQSGPASAEIKSAD 151

Query: 220 DAERILTSESKVAVGYLNSLVGSESDE-LAAASRLEDDVNFYQTVDPEVAKLFHIEASAK 279
           DA  +++ +  V VG    L GSE D  +A A +L  +++F  T D ++  L   E+S  
Sbjct: 152 DASEVVSDKKVVVVGIFPKLSGSEFDSFMAIAEKLRSELDFAHTSDAKL--LPRGESSVT 211

Query: 280 RPALVLLKKEAEKL---SRFDGEFSKSAIVEFVFANKLPLVTMFTKE--NAPLI---FES 339
            P + L K   E+      FDGE    A+ +FV  + +PL+T+F K+  N P +   FES
Sbjct: 212 GPVVRLFKPFDEQFVDSKDFDGE----ALEKFVKESSIPLITVFDKDPNNHPYVIKFFES 271

Query: 340 SIKKQLILFAISND-TEKLIPIFEEAAKSFKGK-LIFVYVEIDNEEVGKPVSEYFGVNGN 399
           +  K ++    + +  E L   + E A S KG+ L F+  + +N +      +YFG+  +
Sbjct: 272 TNTKAMLFINFTGEGAESLKSKYREVATSNKGQGLSFLLGDAENSQ---GAFQYFGLEES 331

Query: 400 G-PEVLGYTGNEDSKKFVLDKEVTLENIKAFAENFLEDKLKPFYKSDPIPETNDGDVKIV 459
             P ++  T   D KK+ L   V ++ I+++ ++F + K+ P  KS PIP  N+  VK+V
Sbjct: 332 QVPLIIIQTA--DDKKY-LKTNVEVDQIESWVKDFKDGKIAPHKKSQPIPAENNEPVKVV 391

Query: 460 VGDNFDEIVLDESKDVLLEIYAPWCGHCQALEPTYNKLAKHLHGVDSLVIAKMDGTTNEH 519
           V D+ D+IVL+  K+VLLE YAPWCGHCQ L P  +++A       S+VIAK+D T N+ 
Sbjct: 392 VSDSLDDIVLNSGKNVLLEFYAPWCGHCQKLAPILDEVAVSYQSDSSVVIAKLDATANDF 451

Query: 520 PRAKSD--GFPTILFFPAGNKSFDPITVDTDRTVVAFYKFLKKNASIPFKLQKPVSSPKA 579
           P+   D  GFPTI F  A   S + +  + DRT   F  F+ KN       +  V  PK 
Sbjct: 452 PKDTFDVKGFPTIYFKSA---SGNVVVYEGDRTKEDFISFVDKN-------KDTVGEPK- 501

Query: 580 ESSEGKSSDDAKESPKSTDLKDEL 584
                      KE   + ++KDEL
Sbjct: 512 -----------KEEETTEEVKDEL 501


HSP 2 Score: 83.2 bits (204), Expect = 5.9e-16
Identity = 54/169 (31.95%), Postives = 84/169 (49.70%), Query Frame = 1

Query: 38  DDDDEDLSFLEEPDEAHAHQNLPDFDNFEGGAEDEDFGDFTDFEDAEG----DGDEYKAP 97
           ++    L  ++  D+    +   + D  E   +D   G     + ++     + +  K  
Sbjct: 320 EESQVPLIIIQTADDKKYLKTNVEVDQIESWVKDFKDGKIAPHKKSQPIPAENNEPVKVV 379

Query: 98  VVDEKDVVVLKEGNFSDFIKKNRFVMVEFYAPWCGHCQALAPEYAAAATELKAE-NVALA 157
           V D  D +VL  G       KN  V++EFYAPWCGHCQ LAP     A   +++ +V +A
Sbjct: 380 VSDSLDDIVLNSG-------KN--VLLEFYAPWCGHCQKLAPILDEVAVSYQSDSSVVIA 439

Query: 158 KVDATEENELAQQYDVQGFPTVYFFS-DGVHKAYPGQRTKDAIVSWIKK 201
           K+DAT  +     +DV+GFPT+YF S  G    Y G RTK+  +S++ K
Sbjct: 440 KLDATANDFPKDTFDVKGFPTIYFKSASGNVVVYEGDRTKEDFISFVDK 479

BLAST of Cucsa.226930 vs. NCBI nr
Match: gi|449462091|ref|XP_004148775.1| (PREDICTED: protein disulfide isomerase-like 1-4 [Cucumis sativus])

HSP 1 Score: 1165.2 bits (3013), Expect = 0.0e+00
Identity = 583/583 (100.00%), Postives = 583/583 (100.00%), Query Frame = 1

Query: 1   MATRTLLLLAMAALLLFSNSVLCKDDSPPKDPLSKPTDDDDEDLSFLEEPDEAHAHQNLP 60
           MATRTLLLLAMAALLLFSNSVLCKDDSPPKDPLSKPTDDDDEDLSFLEEPDEAHAHQNLP
Sbjct: 1   MATRTLLLLAMAALLLFSNSVLCKDDSPPKDPLSKPTDDDDEDLSFLEEPDEAHAHQNLP 60

Query: 61  DFDNFEGGAEDEDFGDFTDFEDAEGDGDEYKAPVVDEKDVVVLKEGNFSDFIKKNRFVMV 120
           DFDNFEGGAEDEDFGDFTDFEDAEGDGDEYKAPVVDEKDVVVLKEGNFSDFIKKNRFVMV
Sbjct: 61  DFDNFEGGAEDEDFGDFTDFEDAEGDGDEYKAPVVDEKDVVVLKEGNFSDFIKKNRFVMV 120

Query: 121 EFYAPWCGHCQALAPEYAAAATELKAENVALAKVDATEENELAQQYDVQGFPTVYFFSDG 180
           EFYAPWCGHCQALAPEYAAAATELKAENVALAKVDATEENELAQQYDVQGFPTVYFFSDG
Sbjct: 121 EFYAPWCGHCQALAPEYAAAATELKAENVALAKVDATEENELAQQYDVQGFPTVYFFSDG 180

Query: 181 VHKAYPGQRTKDAIVSWIKKKTGPGIYNITSVEDAERILTSESKVAVGYLNSLVGSESDE 240
           VHKAYPGQRTKDAIVSWIKKKTGPGIYNITSVEDAERILTSESKVAVGYLNSLVGSESDE
Sbjct: 181 VHKAYPGQRTKDAIVSWIKKKTGPGIYNITSVEDAERILTSESKVAVGYLNSLVGSESDE 240

Query: 241 LAAASRLEDDVNFYQTVDPEVAKLFHIEASAKRPALVLLKKEAEKLSRFDGEFSKSAIVE 300
           LAAASRLEDDVNFYQTVDPEVAKLFHIEASAKRPALVLLKKEAEKLSRFDGEFSKSAIVE
Sbjct: 241 LAAASRLEDDVNFYQTVDPEVAKLFHIEASAKRPALVLLKKEAEKLSRFDGEFSKSAIVE 300

Query: 301 FVFANKLPLVTMFTKENAPLIFESSIKKQLILFAISNDTEKLIPIFEEAAKSFKGKLIFV 360
           FVFANKLPLVTMFTKENAPLIFESSIKKQLILFAISNDTEKLIPIFEEAAKSFKGKLIFV
Sbjct: 301 FVFANKLPLVTMFTKENAPLIFESSIKKQLILFAISNDTEKLIPIFEEAAKSFKGKLIFV 360

Query: 361 YVEIDNEEVGKPVSEYFGVNGNGPEVLGYTGNEDSKKFVLDKEVTLENIKAFAENFLEDK 420
           YVEIDNEEVGKPVSEYFGVNGNGPEVLGYTGNEDSKKFVLDKEVTLENIKAFAENFLEDK
Sbjct: 361 YVEIDNEEVGKPVSEYFGVNGNGPEVLGYTGNEDSKKFVLDKEVTLENIKAFAENFLEDK 420

Query: 421 LKPFYKSDPIPETNDGDVKIVVGDNFDEIVLDESKDVLLEIYAPWCGHCQALEPTYNKLA 480
           LKPFYKSDPIPETNDGDVKIVVGDNFDEIVLDESKDVLLEIYAPWCGHCQALEPTYNKLA
Sbjct: 421 LKPFYKSDPIPETNDGDVKIVVGDNFDEIVLDESKDVLLEIYAPWCGHCQALEPTYNKLA 480

Query: 481 KHLHGVDSLVIAKMDGTTNEHPRAKSDGFPTILFFPAGNKSFDPITVDTDRTVVAFYKFL 540
           KHLHGVDSLVIAKMDGTTNEHPRAKSDGFPTILFFPAGNKSFDPITVDTDRTVVAFYKFL
Sbjct: 481 KHLHGVDSLVIAKMDGTTNEHPRAKSDGFPTILFFPAGNKSFDPITVDTDRTVVAFYKFL 540

Query: 541 KKNASIPFKLQKPVSSPKAESSEGKSSDDAKESPKSTDLKDEL 584
           KKNASIPFKLQKPVSSPKAESSEGKSSDDAKESPKSTDLKDEL
Sbjct: 541 KKNASIPFKLQKPVSSPKAESSEGKSSDDAKESPKSTDLKDEL 583

BLAST of Cucsa.226930 vs. NCBI nr
Match: gi|703105231|ref|XP_010098196.1| (Protein disulfide isomerase-like 1-4 [Morus notabilis])

HSP 1 Score: 892.5 bits (2305), Expect = 4.0e-256
Identity = 441/590 (74.75%), Postives = 507/590 (85.93%), Query Frame = 1

Query: 1   MATRTLLLLAMAALLLFSNSVLCKDDSPPKDPLSKPTDDDDEDLSFLEEPDEAH-----A 60
           MA+R  + LA AALL FSNSVLCK+        +K  DDDDEDLSFLEE D+        
Sbjct: 1   MASRLSIALAFAALLFFSNSVLCKESK------AKDLDDDDEDLSFLEESDDKSDANHGG 60

Query: 61  HQNLPDFDNFEGGAEDEDFGDFTDFEDAEGDGDEYKAPVVDEKDVVVLKEGNFSDFIKKN 120
           H + PD DNF+    D+DFG+F+DF++ + D + YK P VD+KDVVVLKE NF+  I+ N
Sbjct: 61  HNHYPDPDNFDDEG-DDDFGNFSDFDEPDADQEAYKEPEVDDKDVVVLKERNFTGVIENN 120

Query: 121 RFVMVEFYAPWCGHCQALAPEYAAAATELKAENVALAKVDATEENELAQQYDVQGFPTVY 180
           +FVMVEFYAPWCGHCQALAPEYAAAATELK ENV LAKVDATEENELA +YDVQG+PT+Y
Sbjct: 121 QFVMVEFYAPWCGHCQALAPEYAAAATELKGENVVLAKVDATEENELAHEYDVQGYPTIY 180

Query: 181 FFSDGVHKAYPGQRTKDAIVSWIKKKTGPGIYNITSVEDAERILTSESKVAVGYLNSLVG 240
           FF DGVHK Y G R K+AIV+WIKKK GPGI NIT+ EDAERILTSESKV + YLNSLVG
Sbjct: 181 FFVDGVHKPYSGARNKEAIVTWIKKKIGPGINNITTTEDAERILTSESKVVLAYLNSLVG 240

Query: 241 SESDELAAASRLEDDVNFYQTVDPEVAKLFHIEASAKRPALVLLKKEAEKLSRFDGEFSK 300
           SESD LA+ASRLEDDVNFYQTV+P+VAKLFHIE   KRPALVLLK+E EKL  FDG+F K
Sbjct: 241 SESDVLASASRLEDDVNFYQTVNPDVAKLFHIEPEVKRPALVLLKEEPEKLIHFDGKFDK 300

Query: 301 SAIVEFVFANKLPLVTMFTKENAPLIFESSIKKQLILFAISNDTEKLIPIFEEAAKSFKG 360
           S+I EFVFANKLPLVT+FT+ENAP +FES+IKKQL+LF+  NDTEK++PIF+EAAKSFKG
Sbjct: 301 SSISEFVFANKLPLVTIFTRENAPAVFESTIKKQLLLFSTKNDTEKVVPIFQEAAKSFKG 360

Query: 361 KLIFVYVEIDNEEVGKPVSEYFGVNGNGPEVLGYTGNEDSKKFVLDKEVTLENIKAFAEN 420
           KLIFVY+E+DNE+VGKPVSEYFGV+G+ P+V+ YTGN+D KKFVLD EVT++NIKAF E+
Sbjct: 361 KLIFVYIEMDNEDVGKPVSEYFGVSGDAPKVIAYTGNDDGKKFVLDGEVTVDNIKAFGED 420

Query: 421 FLEDKLKPFYKSDPIPETNDGDVKIVVGDNFDEIVLDESKDVLLEIYAPWCGHCQALEPT 480
           FL+DKLKPF KSDPIPETNDGDVKI+VG+NFDEIVLDESKDVLLEIYAPWCGHCQALEPT
Sbjct: 421 FLQDKLKPFLKSDPIPETNDGDVKIIVGNNFDEIVLDESKDVLLEIYAPWCGHCQALEPT 480

Query: 481 YNKLAKHLHGVDSLVIAKMDGTTNEHPRAKSDGFPTILFFPAGNKSFDPITVDTDRTVVA 540
           YNKLAKHL G++S+VIAKMDGTTNEHPRAKSDGFPTILFFPAGNKSFDPITVDTDRTVVA
Sbjct: 481 YNKLAKHLRGIESIVIAKMDGTTNEHPRAKSDGFPTILFFPAGNKSFDPITVDTDRTVVA 540

Query: 541 FYKFLKKNASIPFKLQKPVSSPKAESSEGKSSDDAKESPKST--DLKDEL 584
           FYKFLKK+ASIPFKLQKP +SPK+ES + K + D  +S +++  DLKDEL
Sbjct: 541 FYKFLKKHASIPFKLQKPTTSPKSESPDAKETSDGTQSTETSTKDLKDEL 583

BLAST of Cucsa.226930 vs. NCBI nr
Match: gi|702272649|ref|XP_010043744.1| (PREDICTED: protein disulfide isomerase-like 1-4 [Eucalyptus grandis])

HSP 1 Score: 874.4 bits (2258), Expect = 1.1e-250
Identity = 440/596 (73.83%), Postives = 508/596 (85.23%), Query Frame = 1

Query: 1   MATRTLLLL-AMAALLLFSNSVLCKDDSPPKDPLSKPTD-DDDEDLSFLEEPDEAH---- 60
           M+TR LLLL ++AALLL +        S P+   +   D DDDEDLSFLEEP+E H    
Sbjct: 1   MSTRVLLLLLSLAALLLVA--------SFPRSLSAAAVDVDDDEDLSFLEEPEEGHGGAG 60

Query: 61  ------AHQNLPDFDNFEGGAEDEDFGDFTDFEDAEGDGDEYKAPVVDEKDVVVLKEGNF 120
                 AH   PD D+FE   +DEDF +++D +++E D D YK P VD+KDVVVLKEGNF
Sbjct: 61  GAAAAGAHDEFPDSDHFE---DDEDFENYSDLDESEADADAYKEPEVDDKDVVVLKEGNF 120

Query: 121 SDFIKKNRFVMVEFYAPWCGHCQALAPEYAAAATELKAENVALAKVDATEENELAQQYDV 180
           SDF++KNRFVMVEFYAPWCGHCQALAPEYAAAATELK E V LAKVDATEE ELAQ+Y+V
Sbjct: 121 SDFVEKNRFVMVEFYAPWCGHCQALAPEYAAAATELKGEEVVLAKVDATEEEELAQKYEV 180

Query: 181 QGFPTVYFFSDGVHKAYPGQRTKDAIVSWIKKKTGPGIYNITSVEDAERILTSESKVAVG 240
           QGFPTVYFF DGVHK Y GQRTKDAIV+WIKKKTGPG+ N+T+ ++AER+LTSE+KV +G
Sbjct: 181 QGFPTVYFFVDGVHKPYSGQRTKDAIVTWIKKKTGPGVNNLTTADEAERVLTSENKVVLG 240

Query: 241 YLNSLVGSESDELAAASRLEDDVNFYQTVDPEVAKLFHIEASAKRPALVLLKKEAEKLSR 300
           YLN L G ES+ELAAASR+EDDVNFYQTV+P+VAKLFHI+   KRPALV+LKKEAEKLS 
Sbjct: 241 YLNHLKGPESEELAAASRIEDDVNFYQTVNPDVAKLFHIDPEVKRPALVILKKEAEKLSL 300

Query: 301 FDGEFSKSAIVEFVFANKLPLVTMFTKENAPLIFESSIKKQLILFAISNDTEKLIPIFEE 360
           FDG+F KSAI EFVFANKLPLVT FT+E+APLIFES +KKQ++LFA S D+EK  P+FEE
Sbjct: 301 FDGKFEKSAIAEFVFANKLPLVTTFTRESAPLIFESPVKKQVLLFATSKDSEKFFPVFEE 360

Query: 361 AAKSFKGKLIFVYVEIDNEEVGKPVSEYFGVNGNGPEVLGYTGNEDSKKFVLDKEVTLEN 420
           AAK FKGKLIFVYVE+DNE+VGKPVS+YFGV+G+GP+VLGYTGN+DS+KF LD EVTL+N
Sbjct: 361 AAKLFKGKLIFVYVEMDNEDVGKPVSDYFGVSGSGPKVLGYTGNDDSRKFFLDGEVTLDN 420

Query: 421 IKAFAENFLEDKLKPFYKSDPIPETNDGDVKIVVGDNFDEIVLDESKDVLLEIYAPWCGH 480
           IKAFA +FLE+KLKPFYKSDPIPETNDGDVK+VVG+NFDEIVLDESKDVLLEI+APWCGH
Sbjct: 421 IKAFALDFLEEKLKPFYKSDPIPETNDGDVKVVVGNNFDEIVLDESKDVLLEIHAPWCGH 480

Query: 481 CQALEPTYNKLAKHLHGVDSLVIAKMDGTTNEHPRAKSDGFPTILFFPAGNKSFDPITVD 540
           CQALEPTY+KLAKHL G+DSLVIAKMDGTTNEHPRAK+DGFPT+LFFPAGNKSFDPI+VD
Sbjct: 481 CQALEPTYDKLAKHLRGIDSLVIAKMDGTTNEHPRAKADGFPTLLFFPAGNKSFDPISVD 540

Query: 541 TDRTVVAFYKFLKKNASIPFKLQKP-VSSPKAESSEGKSSDDAKESPKSTDLKDEL 584
           +DRTVVA YKFLKKNASIPFKLQKP  S+PK  +++ K S    E   +TDLKDEL
Sbjct: 541 SDRTVVALYKFLKKNASIPFKLQKPAASTPKPATTDTKES----EETVTTDLKDEL 581

BLAST of Cucsa.226930 vs. NCBI nr
Match: gi|1009115767|ref|XP_015874407.1| (PREDICTED: protein disulfide isomerase-like 1-4 [Ziziphus jujuba])

HSP 1 Score: 874.0 bits (2257), Expect = 1.5e-250
Identity = 441/586 (75.26%), Postives = 501/586 (85.49%), Query Frame = 1

Query: 1   MATRTLLLLAMAALLLFSNSVLCKDDSPPKDPLSKPTDDDDEDLSFLEEPDEAHAH--QN 60
           MA+R +LLLA+ +LLLFSNSVLCKD        +K +++DDEDLSFLEE D+        
Sbjct: 1   MASRLVLLLALTSLLLFSNSVLCKDSK------TKESEEDDEDLSFLEEADDQTVPPPHR 60

Query: 61  LPDFDNFEGGAEDEDFGDFTDFEDAEGDGDE-YKAPVVDEKDVVVLKEGNFSDFIKKNRF 120
            PD D  +    D DF +F DF+ +  D DE YK P VD+KDVVVLKE NF+D I+ N+F
Sbjct: 61  YPDLDQSDDEG-DGDFDNFADFDGSVADQDEAYKEPEVDDKDVVVLKERNFTDVIENNKF 120

Query: 121 VMVEFYAPWCGHCQALAPEYAAAATELKAENVALAKVDATEENELAQQYDVQGFPTVYFF 180
           VMVEFYAPWCGHCQALAPEYAAAATELK E+V LAK+DATEENEL+Q+YDVQGFPT+YFF
Sbjct: 121 VMVEFYAPWCGHCQALAPEYAAAATELKGEDVVLAKIDATEENELSQEYDVQGFPTIYFF 180

Query: 181 SDGVHKAYPGQRTKDAIVSWIKKKTGPGIYNITSVEDAERILTSESKVAVGYLNSLVGSE 240
            DGVHK Y GQRTK+AIV+WIKKK GPGI+NIT+++DAER+LTSESKV +G+LNSLVG E
Sbjct: 181 IDGVHKPYTGQRTKEAIVTWIKKKIGPGIHNITTLDDAERVLTSESKVVLGFLNSLVGPE 240

Query: 241 SDELAAASRLEDDVNFYQTVDPEVAKLFHIEASAKRPALVLLKKEAEKLSRFDGEFSKSA 300
           SDELAAASRLEDDVNFYQTVDP VAKLFHI+   KRPALVLLKKE EKLS FDG F KS+
Sbjct: 241 SDELAAASRLEDDVNFYQTVDPAVAKLFHIDPEVKRPALVLLKKETEKLSHFDGPFVKSS 300

Query: 301 IVEFVFANKLPLVTMFTKENAPLIFESSIKKQLILFAISNDTEKLIPIFEEAAKSFKGKL 360
           I EFVFANKLPLVT+FT+E+AP IFES IKKQ++LFA SND++++IP FEEAAK FKGKL
Sbjct: 301 IAEFVFANKLPLVTIFTRESAPTIFESPIKKQILLFATSNDSKEVIPAFEEAAKLFKGKL 360

Query: 361 IFVYVEIDNEEVGKPVSEYFGVNGNGPEVLGYTGNEDSKKFVLDKEVTLENIKAFAENFL 420
           IFV+VE+DNE+VGKPVSEYFGV+ + P VL YTGN+D++KFV D EVTLENIKAF ENFL
Sbjct: 361 IFVFVEMDNEDVGKPVSEYFGVS-DAPTVLAYTGNDDARKFVHDGEVTLENIKAFGENFL 420

Query: 421 EDKLKPFYKSDPIPETNDGDVKIVVGDNFDEIVLDESKDVLLEIYAPWCGHCQALEPTYN 480
           EDKLKPF+KSDPIPETND DVKIVVG+NFD+IVLDESKDVLLEIYAPWCGHCQALEPTYN
Sbjct: 421 EDKLKPFFKSDPIPETNDEDVKIVVGNNFDDIVLDESKDVLLEIYAPWCGHCQALEPTYN 480

Query: 481 KLAKHLHGVDSLVIAKMDGTTNEHPRAKSDGFPTILFFPAGNKSFDPITVDTDRTVVAFY 540
           KLAKHL G++SLVIAKMDGTTNEHPRAK+DGFP +LFFPAGNKSFDPITVDTDRTV A Y
Sbjct: 481 KLAKHLRGIESLVIAKMDGTTNEHPRAKADGFPALLFFPAGNKSFDPITVDTDRTVTALY 540

Query: 541 KFLKKNASIPFKLQKPVSSPKAESSEGKSSDDAKESPKSTDLKDEL 584
           KFLKKNASIPFKLQKP SSPK  SS+GK S D+     STD+KDEL
Sbjct: 541 KFLKKNASIPFKLQKPTSSPKPVSSDGKESADS----SSTDVKDEL 574

BLAST of Cucsa.226930 vs. NCBI nr
Match: gi|743902057|ref|XP_011044358.1| (PREDICTED: protein disulfide isomerase-like 1-4 [Populus euphratica])

HSP 1 Score: 870.9 bits (2249), Expect = 1.2e-249
Identity = 435/593 (73.36%), Postives = 509/593 (85.83%), Query Frame = 1

Query: 1   MATRTLLLLAMAALLLFSNSVLCKDDSPPKDPLSKPT-DDDDEDLSFLEEPDEA------ 60
           M+TR ++LL++  LLLFS        SP    L     +DDDEDLSFLEE  +A      
Sbjct: 1   MSTRFIVLLSLTVLLLFSQL------SPSVSKLQNAAAEDDDEDLSFLEEETDAVPQGHG 60

Query: 61  --HAHQNLPDFDNFEGGAEDED-FGDFTDFEDAEGDGDEYKAPVVDEKDVVVLKEGNFSD 120
             H H + PD D F+   ++ED   +++D +D+E D   YK P +D+KDVVVLKEGNFSD
Sbjct: 61  HEHEHDHYPDPDQFDEEFDNEDDLDNYSDLDDSELDS--YKEPEIDDKDVVVLKEGNFSD 120

Query: 121 FIKKNRFVMVEFYAPWCGHCQALAPEYAAAATELKAENVALAKVDATEENELAQQYDVQG 180
           F+ KN+FVMVEFYAPWCGHCQ+LAPEYAAAATELKAE V LAKVDATEENELAQ+YD+QG
Sbjct: 121 FVTKNKFVMVEFYAPWCGHCQSLAPEYAAAATELKAEGVMLAKVDATEENELAQEYDIQG 180

Query: 181 FPTVYFFSDGVHKAYPGQRTKDAIVSWIKKKTGPGIYNITSVEDAERILTSESKVAVGYL 240
           FPTVYFF DGVHK YPG RTK+ IV+WIKKK GPGIYNIT+V+DAER LTSE+K+ +G+L
Sbjct: 181 FPTVYFFVDGVHKPYPGPRTKEGIVTWIKKKIGPGIYNITTVDDAERFLTSETKLVLGFL 240

Query: 241 NSLVGSESDELAAASRLEDDVNFYQTVDPEVAKLFHIEASAKRPALVLLKKEAEKLSRFD 300
           NSLVG ES+ELAAASRLED+V+FYQTV+P+VAKLFH++  AKRPALV+LKKEAEKLS FD
Sbjct: 241 NSLVGPESEELAAASRLEDEVSFYQTVNPDVAKLFHLDPQAKRPALVMLKKEAEKLSVFD 300

Query: 301 GEFSKSAIVEFVFANKLPLVTMFTKENAPLIFESSIKKQLILFAISNDTEKLIPIFEEAA 360
           G FSKS I EFVFANKLPLVT+FT+ENAPLIFES+IKKQL+LFAISND+EK++PIF+EAA
Sbjct: 301 GNFSKSEIAEFVFANKLPLVTIFTRENAPLIFESTIKKQLLLFAISNDSEKVVPIFQEAA 360

Query: 361 KSFKGKLIFVYVEIDNEEVGKPVSEYFGVNGNGPEVLGYTGNEDSKKFVLDKEVTLENIK 420
           + FKGKLIFVYVE+DNE+VGKPVSEYFG++G  P+VL YTGN+D+KKFVLD +VTL+NIK
Sbjct: 361 RLFKGKLIFVYVEMDNEDVGKPVSEYFGISGTAPKVLAYTGNDDAKKFVLDGDVTLDNIK 420

Query: 421 AFAENFLEDKLKPFYKSDPIPETNDGDVKIVVGDNFDEIVLDESKDVLLEIYAPWCGHCQ 480
           AF E+F+EDKLKPF+KSDP+PE+NDGDVKIVVGDNFDEIVLDESKDVLLEIYAPWCGHCQ
Sbjct: 421 AFGEDFIEDKLKPFFKSDPVPESNDGDVKIVVGDNFDEIVLDESKDVLLEIYAPWCGHCQ 480

Query: 481 ALEPTYNKLAKHLHGVDSLVIAKMDGTTNEHPRAKSDGFPTILFFPAGNKSFDPITVDTD 540
           +LEPTYNKLA HL G++S+VIAKMDGTTNEHPRAKSDGFPT+LFFPAGNKSFDPITVDTD
Sbjct: 481 SLEPTYNKLAAHLRGIESIVIAKMDGTTNEHPRAKSDGFPTLLFFPAGNKSFDPITVDTD 540

Query: 541 RTVVAFYKFLKKNASIPFKLQKPVSSPKAESSEGKSSDDAKESPKSTDLKDEL 584
           RTVVAFYKF+KK+ASIPFKLQKP S+ KAESS+ K   D  ES  + ++KDEL
Sbjct: 541 RTVVAFYKFIKKHASIPFKLQKPASTSKAESSDAK---DGIES-STREVKDEL 581

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PDI14_ARATH2.6e-22364.30Protein disulfide isomerase-like 1-4 OS=Arabidopsis thaliana GN=PDIL1-4 PE=1 SV=... [more]
PDI13_ARATH5.2e-20062.41Protein disulfide isomerase-like 1-3 OS=Arabidopsis thaliana GN=PDIL1-3 PE=2 SV=... [more]
PDI14_ORYSJ3.6e-19359.52Protein disulfide isomerase-like 1-4 OS=Oryza sativa subsp. japonica GN=PDIL1-4 ... [more]
PDIA1_CHICK1.3e-8940.09Protein disulfide-isomerase OS=Gallus gallus GN=P4HB PE=1 SV=3[more]
PDIA1_BOVIN1.3e-8938.52Protein disulfide-isomerase OS=Bos taurus GN=P4HB PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LUQ2_CUCSA0.0e+00100.00Protein disulfide-isomerase OS=Cucumis sativus GN=Csa_1G084280 PE=3 SV=1[more]
W9RV77_9ROSA2.8e-25674.75Protein disulfide-isomerase OS=Morus notabilis GN=L484_007658 PE=3 SV=1[more]
A0A059D5F7_EUCGR7.8e-25173.83Protein disulfide-isomerase OS=Eucalyptus grandis GN=EUGRSUZ_B02496 PE=3 SV=1[more]
B9HQ95_POPTR8.0e-24872.61Protein disulfide-isomerase OS=Populus trichocarpa GN=POPTR_0009s01920g PE=3 SV=... [more]
M5W6Y1_PRUPE4.0e-24773.21Protein disulfide-isomerase OS=Prunus persica GN=PRUPE_ppa003432mg PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G60640.11.5e-22464.30 PDI-like 1-4[more]
AT3G54960.13.0e-20162.41 PDI-like 1-3[more]
AT1G52260.12.9e-7934.01 PDI-like 1-5[more]
AT3G16110.13.7e-7935.09 PDI-like 1-6[more]
AT1G21750.13.6e-7437.50 PDI-like 1-1[more]
Match NameE-valueIdentityDescription
gi|449462091|ref|XP_004148775.1|0.0e+00100.00PREDICTED: protein disulfide isomerase-like 1-4 [Cucumis sativus][more]
gi|703105231|ref|XP_010098196.1|4.0e-25674.75Protein disulfide isomerase-like 1-4 [Morus notabilis][more]
gi|702272649|ref|XP_010043744.1|1.1e-25073.83PREDICTED: protein disulfide isomerase-like 1-4 [Eucalyptus grandis][more]
gi|1009115767|ref|XP_015874407.1|1.5e-25075.26PREDICTED: protein disulfide isomerase-like 1-4 [Ziziphus jujuba][more]
gi|743902057|ref|XP_011044358.1|1.2e-24973.36PREDICTED: protein disulfide isomerase-like 1-4 [Populus euphratica][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR005788Disulphide_isomerase
IPR005792Prot_disulphide_isomerase
IPR012336Thioredoxin-like_fold
IPR013766Thioredoxin_domain
IPR017937Thioredoxin_CS
IPR005788Disulphide_isomerase
IPR005792Prot_disulphide_isomerase
IPR012336Thioredoxin-like_fold
IPR013766Thioredoxin_domain
IPR017937Thioredoxin_CS
Vocabulary: Molecular Function
TermDefinition
GO:0016853isomerase activity
GO:0016853isomerase activity
Vocabulary: Cellular Component
TermDefinition
GO:0005783endoplasmic reticulum
GO:0005783endoplasmic reticulum
Vocabulary: Biological Process
TermDefinition
GO:0045454cell redox homeostasis
GO:0045454cell redox homeostasis
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045454 cell redox homeostasis
biological_process GO:0009408 response to heat
biological_process GO:0009627 systemic acquired resistance
biological_process GO:0030244 cellulose biosynthetic process
biological_process GO:0042542 response to hydrogen peroxide
biological_process GO:0009644 response to high light intensity
biological_process GO:0009744 response to sucrose
biological_process GO:0009749 response to glucose
biological_process GO:0009750 response to fructose
biological_process GO:0006457 protein folding
biological_process GO:0006499 N-terminal protein myristoylation
biological_process GO:0048193 Golgi vesicle transport
cellular_component GO:0009507 chloroplast
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0005739 mitochondrion
cellular_component GO:0005774 vacuolar membrane
cellular_component GO:0005575 cellular_component
molecular_function GO:0003756 protein disulfide isomerase activity
molecular_function GO:0016757 transferase activity, transferring glycosyl groups
molecular_function GO:0016853 isomerase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.226930.2Cucsa.226930.2mRNA
Cucsa.226930.1Cucsa.226930.1mRNA


Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005788Disulphide isomeraseTIGRFAMsTIGR01126TIGR01126coord: 104..201
score: 3.4
IPR005792Protein disulphide isomeraseTIGRFAMsTIGR01130TIGR01130coord: 98..548
score: 3.9E
IPR012336Thioredoxin-like foldGENE3DG3DSA:3.40.30.10coord: 319..403
score: 2.6E-6coord: 414..525
score: 1.6E-30coord: 96..204
score: 9.1E-41coord: 209..296
score: 8.
IPR012336Thioredoxin-like foldunknownSSF52833Thioredoxin-likecoord: 94..203
score: 8.47E-37coord: 207..296
score: 1.16E-6coord: 288..415
score: 5.95E-23coord: 415..525
score: 4.41
IPR013766Thioredoxin domainPFAMPF00085Thioredoxincoord: 418..502
score: 1.3E-20coord: 100..200
score: 1.5
IPR013766Thioredoxin domainPROFILEPS51352THIOREDOXIN_2coord: 396..525
score: 12.237coord: 68..203
score: 17
IPR017937Thioredoxin, conserved sitePROSITEPS00194THIOREDOXIN_1coord: 119..137
score: -coord: 438..456
scor
NoneNo IPR availablePRINTSPR00421THIOREDOXINcoord: 118..126
score: 1.6E-6coord: 167..178
score: 1.6E-6coord: 126..135
score: 1.
NoneNo IPR availablePANTHERPTHR18929PROTEIN DISULFIDE ISOMERASEcoord: 1..31
score: 3.1E-179coord: 100..227
score: 3.1E-179coord: 310..552
score: 3.1E
NoneNo IPR availablePANTHERPTHR18929:SF74PROTEIN DISULFIDE-ISOMERASE A3coord: 1..31
score: 3.1E-179coord: 100..227
score: 3.1E-179coord: 310..552
score: 3.1E
NoneNo IPR availablePFAMPF13848Thioredoxin_6coord: 284..395
score: 1.7