Cucsa.220370 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.220370 vs. Swiss-Prot
Match: ISPH_ORYSJ (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic OS=Oryza sativa subsp. japonica GN=ISPH PE=2 SV=1)

HSP 1 Score: 744.2 bits (1920), Expect = 8.7e-214
Identity = 361/438 (82.42%), Postives = 409/438 (93.38%), Query Frame = 1

Query: 31  RKPMSVRCDGDSSSSSSSSSSSAAVDSNFDAKVFRRNLTKSDNYNRKGFGYKDETLALMN 90
           R P SVRCD   SS++SS S+SA++D++FD K FR NLT+SDNYNRKGFG+K ETL LM+
Sbjct: 25  RAPSSVRCD---SSAASSLSASASLDADFDKKQFRHNLTRSDNYNRKGFGHKKETLELMS 84

Query: 91  REYTSDIIKTLKENGFEYTWGDVTVKLAEAYGFCWGVERAVQIAYEARKQFPDEKIWLTN 150
           +EYTSD+IKTLKENG ++TWG VTVKLAEAYGFCWGVERAVQIAYEARKQFPD++IWLTN
Sbjct: 85  QEYTSDVIKTLKENGNQHTWGPVTVKLAEAYGFCWGVERAVQIAYEARKQFPDDRIWLTN 144

Query: 151 EIIHNPTVNKRLEEMEVQNIPLEDGRKQFEVVDKGDVVILPAFGAAVDEMLTLSEKKVQI 210
           EIIHNPTVNKRLE+M VQNIP++ G K F+VV++GDVV+LPAFGAAV+EM TL+EKKVQI
Sbjct: 145 EIIHNPTVNKRLEDMGVQNIPVDAGIKDFDVVEQGDVVVLPAFGAAVEEMYTLNEKKVQI 204

Query: 211 VDTTCPWVSKVWNVVDKHKKGDYTSIIHGKYAHEETVATASFAGTFIIVKNMAEAIYVCD 270
           VDTTCPWVSKVWN+V+KHKKGDYTSIIHGKY+HEETVATASFAGT+IIVKN+AEA YVCD
Sbjct: 205 VDTTCPWVSKVWNMVEKHKKGDYTSIIHGKYSHEETVATASFAGTYIIVKNIAEASYVCD 264

Query: 271 YILGGELDGSSSTKEEFMKKFKNAVSKGFDPDVDLLKVGIANQTTMLKGETEEIGKLVER 330
           YILGG+LDGSSSTKEEF++KFKNAVS GFDPDVDL+KVGIANQTTMLKGETEEIGKLVE+
Sbjct: 265 YILGGQLDGSSSTKEEFLEKFKNAVSPGFDPDVDLVKVGIANQTTMLKGETEEIGKLVEK 324

Query: 331 TMMRKHGVENVNEHFLSFNTICDATQERQDAMYKLVEGKLDLMLVVGGWNSSNTSHLQEI 390
           TMMR+ GVENVN+HF++FNTICDATQERQDAMY+LV+ K+DL+LVVGGWNSSNTSHLQEI
Sbjct: 325 TMMRRFGVENVNDHFIAFNTICDATQERQDAMYQLVKEKVDLILVVGGWNSSNTSHLQEI 384

Query: 391 AEERGIPSYWIDSEQRIGPGNRIAYKLNHGELVEKENWLPKGHITIGVTSGASTPDKVVE 450
            E  GIPSYWIDSEQRIGPGN+I+YKLNHGELVEKENWLP+G ITIGVTSGASTPDKVVE
Sbjct: 385 GELSGIPSYWIDSEQRIGPGNKISYKLNHGELVEKENWLPEGPITIGVTSGASTPDKVVE 444

Query: 451 DALIKVFDIKREEALQFA 469
           DAL KVF+IKR+E LQ A
Sbjct: 445 DALQKVFEIKRQEVLQAA 459

BLAST of Cucsa.220370 vs. Swiss-Prot
Match: ISPH_ARATH (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic OS=Arabidopsis thaliana GN=ISPH PE=2 SV=1)

HSP 1 Score: 743.4 bits (1918), Expect = 1.5e-213
Identity = 363/469 (77.40%), Postives = 419/469 (89.34%), Query Frame = 1

Query: 1   MAFSMQLYRLPTRTDAAMPEN-FTAVRSLRYRKPMSVRCDGDSSSSSSSSSSSAAVDSNF 60
           MA ++Q  RL  R D  + EN  +   SLR RK +SVRC   SS   ++ S S  +DS+F
Sbjct: 1   MAVALQFSRLCVRPDTFVRENHLSGSGSLRRRKALSVRC---SSGDENAPSPSVVMDSDF 60

Query: 61  DAKVFRRNLTKSDNYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYTWGDVTVKLAE 120
           DAKVFR+NLT+SDNYNRKGFG+K+ETL LMNREYTSDI++TLK NG+ Y+WGDVTVKLA+
Sbjct: 61  DAKVFRKNLTRSDNYNRKGFGHKEETLKLMNREYTSDILETLKTNGYTYSWGDVTVKLAK 120

Query: 121 AYGFCWGVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPLEDGRKQF 180
           AYGFCWGVERAVQIAYEARKQFP+E++W+TNEIIHNPTVNKRLE+M+V+ IP+ED +KQF
Sbjct: 121 AYGFCWGVERAVQIAYEARKQFPEERLWITNEIIHNPTVNKRLEDMDVKIIPVEDSKKQF 180

Query: 181 EVVDKGDVVILPAFGAAVDEMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIHG 240
           +VV+K DVVILPAFGA VDEM  L++KKVQIVDTTCPWV+KVWN V+KHKKG+YTS+IHG
Sbjct: 181 DVVEKDDVVILPAFGAGVDEMYVLNDKKVQIVDTTCPWVTKVWNTVEKHKKGEYTSVIHG 240

Query: 241 KYAHEETVATASFAGTFIIVKNMAEAIYVCDYILGGELDGSSSTKEEFMKKFKNAVSKGF 300
           KY HEET+ATASFAG +IIVKNM EA YVCDYILGG+ DGSSSTKEEFM+KFK A+SKGF
Sbjct: 241 KYNHEETIATASFAGKYIIVKNMKEANYVCDYILGGQYDGSSSTKEEFMEKFKYAISKGF 300

Query: 301 DPDVDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQ 360
           DPD DL+KVGIANQTTMLKGETEEIG+L+E TMMRK+GVENV+ HF+SFNTICDATQERQ
Sbjct: 301 DPDNDLVKVGIANQTTMLKGETEEIGRLLETTMMRKYGVENVSGHFISFNTICDATQERQ 360

Query: 361 DAMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNH 420
           DA+Y+LVE K+DLMLVVGGWNSSNTSHLQEI+E RGIPSYWIDSE+RIGPGN+IAYKL++
Sbjct: 361 DAIYELVEEKIDLMLVVGGWNSSNTSHLQEISEARGIPSYWIDSEKRIGPGNKIAYKLHY 420

Query: 421 GELVEKENWLPKGHITIGVTSGASTPDKVVEDALIKVFDIKREEALQFA 469
           GELVEKEN+LPKG ITIGVTSGASTPDKVVEDAL+KVFDIKREE LQ A
Sbjct: 421 GELVEKENFLPKGPITIGVTSGASTPDKVVEDALVKVFDIKREELLQLA 466

BLAST of Cucsa.220370 vs. Swiss-Prot
Match: ISPH_CYAA5 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Cyanothece sp. (strain ATCC 51142) GN=ispH PE=3 SV=1)

HSP 1 Score: 532.3 bits (1370), Expect = 5.2e-150
Identity = 257/406 (63.30%), Postives = 324/406 (79.80%), Query Frame = 1

Query: 60  DAKVFRRNLTKSDNYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYTWGDVTVKLAE 119
           D K F+R+L +S+NY+RKGFG++ E +  MN EY S +I+ ++EN +++  GDVT+KLAE
Sbjct: 2   DTKAFKRSLQQSNNYHRKGFGHETEVMGTMNTEYQSSLIQKIRENNYQWQEGDVTIKLAE 61

Query: 120 AYGFCWGVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPLEDGRKQF 179
           A+GFCWGVERAV +AYE R+ FP EKIW+TNEIIHNP+VN+RL EM V  I + +G K F
Sbjct: 62  AFGFCWGVERAVAMAYETRQHFPQEKIWITNEIIHNPSVNQRLLEMNVGFIEVINGSKDF 121

Query: 180 EVVDKGDVVILPAFGAAVDEMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIHG 239
            VV+ GDVVILPAFGA+V EM  L++K   IVDTTCPWVSKVWN V+KHKK DYTSIIHG
Sbjct: 122 SVVEAGDVVILPAFGASVTEMQLLNDKGCTIVDTTCPWVSKVWNSVEKHKKKDYTSIIHG 181

Query: 240 KYAHEETVATASFAGTFIIVKNMAEAIYVCDYILGGELDGSSSTKEEFMKKFKNAVSKGF 299
           KY HEETVAT+SFAGT+++V N+ EA YVCDYIL G        K+EF++KFKNA S+GF
Sbjct: 182 KYKHEETVATSSFAGTYLVVLNLKEAQYVCDYILNG------GNKDEFLEKFKNAHSEGF 241

Query: 300 DPDVDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQ 359
           DPD DL+++GIANQTTMLK ETE+IGKL E TM++K+G   +NEHF+SFNTICDATQERQ
Sbjct: 242 DPDKDLIRLGIANQTTMLKSETEQIGKLFETTMLKKYGPTELNEHFMSFNTICDATQERQ 301

Query: 360 DAMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNH 419
           DAM  LVE ++D+M+V+GG+NSSNT+HLQEI+ E+GI SY IDS++RI PGN+I +K   
Sbjct: 302 DAMLNLVEEEVDVMVVIGGFNSSNTTHLQEISIEKGIDSYHIDSDKRILPGNKIEHKPLE 361

Query: 420 GELVEKENWLPKGHITIGVTSGASTPDKVVEDALIKVFDIKREEAL 466
            ++  KENWLP G I +GVTSGASTPDKVVE  + ++F+ K    L
Sbjct: 362 KDIETKENWLPDGKIIVGVTSGASTPDKVVEAVIERIFEEKNAGVL 401

BLAST of Cucsa.220370 vs. Swiss-Prot
Match: ISPH_CYAP4 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Cyanothece sp. (strain PCC 7425 / ATCC 29141) GN=ispH PE=3 SV=1)

HSP 1 Score: 526.6 bits (1355), Expect = 2.9e-148
Identity = 255/402 (63.43%), Postives = 321/402 (79.85%), Query Frame = 1

Query: 60  DAKVFRRNLTKSDNYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYTWGDVTVKLAE 119
           D K F+R+L  S+ Y+RKGFG++ E   ++  EY S +I+ ++ENG  +  G+VT++LAE
Sbjct: 2   DTKAFKRSLHSSNQYHRKGFGHEAEVTEMLRFEYQSSLIQQIRENGNRFQRGEVTIQLAE 61

Query: 120 AYGFCWGVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPL-EDGRKQ 179
           A+GFCWGVERAV +AYE R+ FP E+IW+TNEIIHNP+VN+ L EM+V+ IP+ E G+K 
Sbjct: 62  AFGFCWGVERAVALAYETRQHFPTERIWITNEIIHNPSVNQNLREMQVEFIPVDEQGQKD 121

Query: 180 FEVVDKGDVVILPAFGAAVDEMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIH 239
           F VV   DVVILPAFGA+V EM  L++K  +I+DTTCPWVSKVWN V+KHKKG YTSIIH
Sbjct: 122 FSVVAGEDVVILPAFGASVQEMQLLNDKGCKIMDTTCPWVSKVWNSVEKHKKGSYTSIIH 181

Query: 240 GKYAHEETVATASFAGTFIIVKNMAEAIYVCDYILGGELDGSSSTKEEFMKKFKNAVSKG 299
           GKY HEET+AT+S+A T++IV ++ EA YVC+YIL G        +EEF+ KF++A S  
Sbjct: 182 GKYQHEETIATSSYAATYLIVLDLKEAEYVCNYILNG------GDREEFLHKFRHAYSPD 241

Query: 300 FDPDVDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQER 359
           FDPD DL++VGIANQTTMLKGETE+IGKL ERT+MRK+G + +N+HFLSFNTICDATQER
Sbjct: 242 FDPDRDLVRVGIANQTTMLKGETEQIGKLFERTLMRKYGPDQINQHFLSFNTICDATQER 301

Query: 360 QDAMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLN 419
           QDAM+KLVE  LDLM+V+GG+NSSNT+HLQEI+ ER IPSY IDS  RIGPGNR+ +K  
Sbjct: 302 QDAMFKLVEYPLDLMVVIGGFNSSNTTHLQEISIEREIPSYHIDSADRIGPGNRVEHKPL 361

Query: 420 HGELVEKENWLPKGHITIGVTSGASTPDKVVEDALIKVFDIK 461
           H  L  KENWLP+G I +G+TSGASTPDKVV D L K+F +K
Sbjct: 362 HRPLEIKENWLPEGPIVVGITSGASTPDKVVSDVLEKIFALK 397

BLAST of Cucsa.220370 vs. Swiss-Prot
Match: ISPH_SYNE7 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Synechococcus elongatus (strain PCC 7942) GN=ispH PE=3 SV=1)

HSP 1 Score: 526.2 bits (1354), Expect = 3.7e-148
Identity = 252/402 (62.69%), Postives = 316/402 (78.61%), Query Frame = 1

Query: 60  DAKVFRRNLTKSDNYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYTWGDVTVKLAE 119
           D K F+R L +SD YNRKGFG   +    +   Y SD+I++L++NG+    G++T++LAE
Sbjct: 2   DTKAFKRALHQSDRYNRKGFGKTTDVSGALESAYQSDLIQSLRQNGYRLQRGEITIRLAE 61

Query: 120 AYGFCWGVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPLEDGRKQF 179
           A+GFCWGVERAV IAYE R+ FP E+IW+TNEIIHNP+VN+ L EM V+ IP E G K F
Sbjct: 62  AFGFCWGVERAVAIAYETRQHFPQERIWITNEIIHNPSVNQHLREMSVEFIPCERGEKDF 121

Query: 180 EVVDKGDVVILPAFGAAVDEMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIHG 239
            VVD+GDVVILPAFGA+V EM  L EK   IVDTTCPWVSKVWN V+KHK+G +TSIIHG
Sbjct: 122 SVVDRGDVVILPAFGASVQEMQLLDEKGCHIVDTTCPWVSKVWNTVEKHKRGAHTSIIHG 181

Query: 240 KYAHEETVATASFAGTFIIVKNMAEAIYVCDYILGGELDGSSSTKEEFMKKFKNAVSKGF 299
           KY HEETVAT+SFA T+++V N+ +A YVCDYIL G        ++EFM +F  A S GF
Sbjct: 182 KYNHEETVATSSFAETYLVVLNLEQAQYVCDYILNG------GDRDEFMTRFGKACSAGF 241

Query: 300 DPDVDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQ 359
           DPD DL ++GIANQTTMLK ETE IGKL ERT+++K+G + +N+HFL+FNTICDATQERQ
Sbjct: 242 DPDRDLERIGIANQTTMLKSETEAIGKLFERTLLKKYGPQALNDHFLAFNTICDATQERQ 301

Query: 360 DAMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNH 419
           DAM++LVE  LDL++V+GG+NSSNT+HLQEIA ER IPS+ ID+ +RIGPGNRI +K  H
Sbjct: 302 DAMFQLVEEPLDLIVVIGGFNSSNTTHLQEIAIERQIPSFHIDAAERIGPGNRIEHKPLH 361

Query: 420 GELVEKENWLPKGHITIGVTSGASTPDKVVEDALIKVFDIKR 462
            +L   E WLP G +TIG+TSGASTPDKVVED + ++FD++R
Sbjct: 362 TDLTTTEPWLPAGPLTIGITSGASTPDKVVEDVIERLFDLQR 397

BLAST of Cucsa.220370 vs. TrEMBL
Match: A0A0A0K3D8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G075680 PE=3 SV=1)

HSP 1 Score: 941.4 bits (2432), Expect = 4.2e-271
Identity = 468/468 (100.00%), Postives = 468/468 (100.00%), Query Frame = 1

Query: 1   MAFSMQLYRLPTRTDAAMPENFTAVRSLRYRKPMSVRCDGDSSSSSSSSSSSAAVDSNFD 60
           MAFSMQLYRLPTRTDAAMPENFTAVRSLRYRKPMSVRCDGDSSSSSSSSSSSAAVDSNFD
Sbjct: 1   MAFSMQLYRLPTRTDAAMPENFTAVRSLRYRKPMSVRCDGDSSSSSSSSSSSAAVDSNFD 60

Query: 61  AKVFRRNLTKSDNYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYTWGDVTVKLAEA 120
           AKVFRRNLTKSDNYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYTWGDVTVKLAEA
Sbjct: 61  AKVFRRNLTKSDNYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYTWGDVTVKLAEA 120

Query: 121 YGFCWGVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPLEDGRKQFE 180
           YGFCWGVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPLEDGRKQFE
Sbjct: 121 YGFCWGVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPLEDGRKQFE 180

Query: 181 VVDKGDVVILPAFGAAVDEMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIHGK 240
           VVDKGDVVILPAFGAAVDEMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIHGK
Sbjct: 181 VVDKGDVVILPAFGAAVDEMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIHGK 240

Query: 241 YAHEETVATASFAGTFIIVKNMAEAIYVCDYILGGELDGSSSTKEEFMKKFKNAVSKGFD 300
           YAHEETVATASFAGTFIIVKNMAEAIYVCDYILGGELDGSSSTKEEFMKKFKNAVSKGFD
Sbjct: 241 YAHEETVATASFAGTFIIVKNMAEAIYVCDYILGGELDGSSSTKEEFMKKFKNAVSKGFD 300

Query: 301 PDVDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQD 360
           PDVDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQD
Sbjct: 301 PDVDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQD 360

Query: 361 AMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHG 420
           AMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHG
Sbjct: 361 AMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHG 420

Query: 421 ELVEKENWLPKGHITIGVTSGASTPDKVVEDALIKVFDIKREEALQFA 469
           ELVEKENWLPKGHITIGVTSGASTPDKVVEDALIKVFDIKREEALQFA
Sbjct: 421 ELVEKENWLPKGHITIGVTSGASTPDKVVEDALIKVFDIKREEALQFA 468

BLAST of Cucsa.220370 vs. TrEMBL
Match: K7NBR0_SIRGR (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Siraitia grosvenorii PE=2 SV=1)

HSP 1 Score: 880.2 bits (2273), Expect = 1.1e-252
Identity = 429/470 (91.28%), Postives = 459/470 (97.66%), Query Frame = 1

Query: 1   MAFSMQLYRLPTRTDAAMPENFTAVRSLRYRKPMSVRCDGDSSSSSSSSSSSAAV--DSN 60
           MAFS+QLYRL TRTDAA+PENF+ +R++RYRKP+SVRCDGDSSSSSSSSSSS++V  DSN
Sbjct: 1   MAFSLQLYRLSTRTDAALPENFSGIRAVRYRKPLSVRCDGDSSSSSSSSSSSSSVAMDSN 60

Query: 61  FDAKVFRRNLTKSDNYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYTWGDVTVKLA 120
           FDAKVFR+NLT+S+NYNRKGFGYKDETLALMNREYTSDIIKTLKE+ +EYTWGDVTVKLA
Sbjct: 61  FDAKVFRKNLTRSENYNRKGFGYKDETLALMNREYTSDIIKTLKEHEYEYTWGDVTVKLA 120

Query: 121 EAYGFCWGVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPLEDGRKQ 180
           EAYGFCWGVERAVQIAYEARKQFPDEKIW+TNEIIHNPTVNKRLEEMEV+NIPLE+G+KQ
Sbjct: 121 EAYGFCWGVERAVQIAYEARKQFPDEKIWITNEIIHNPTVNKRLEEMEVENIPLEEGKKQ 180

Query: 181 FEVVDKGDVVILPAFGAAVDEMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIH 240
           FEVV+KGDVVILPAFGAAVDEMLTLSEK VQIVDTTCPWVSKVWNVV+KHKKGD+TSIIH
Sbjct: 181 FEVVNKGDVVILPAFGAAVDEMLTLSEKNVQIVDTTCPWVSKVWNVVEKHKKGDFTSIIH 240

Query: 241 GKYAHEETVATASFAGTFIIVKNMAEAIYVCDYILGGELDGSSSTKEEFMKKFKNAVSKG 300
           GKYAHEETVATASFAG +IIVKNMAEA YVCDYILGGELDGSS TKEEFMKKFKNAVSKG
Sbjct: 241 GKYAHEETVATASFAGKYIIVKNMAEATYVCDYILGGELDGSSPTKEEFMKKFKNAVSKG 300

Query: 301 FDPDVDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQER 360
           FDPD+DL+KVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHF+SFNTICDATQER
Sbjct: 301 FDPDIDLVKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFISFNTICDATQER 360

Query: 361 QDAMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLN 420
           QDAMYKLVEGKLDLMLVVGGWNSSNTSHLQ+IAE+RGIPSYW+DSEQRIGPGN+IAYKLN
Sbjct: 361 QDAMYKLVEGKLDLMLVVGGWNSSNTSHLQQIAEDRGIPSYWVDSEQRIGPGNKIAYKLN 420

Query: 421 HGELVEKENWLPKGHITIGVTSGASTPDKVVEDALIKVFDIKREEALQFA 469
           HGELVEKENWLP+G ITIGVTSGASTPDKVVED LIKVFDIKREEALQFA
Sbjct: 421 HGELVEKENWLPRGPITIGVTSGASTPDKVVEDVLIKVFDIKREEALQFA 470

BLAST of Cucsa.220370 vs. TrEMBL
Match: A0A0G3BEA1_PAELC (Hydroxymethylbutenyl diphosphate reductase OS=Paeonia lactiflora GN=HDR PE=2 SV=1)

HSP 1 Score: 800.8 bits (2067), Expect = 8.8e-229
Identity = 397/468 (84.83%), Postives = 428/468 (91.45%), Query Frame = 1

Query: 1   MAFSMQLYRLPTRTDAAMPENFTAVRSLRYRKPMSVRCDGDSSSSSSSSSSSAAVDSNFD 60
           MA S+QL RL TRTD A P+   A RS   RKP SVRC  DSS S   SSSS AVDS FD
Sbjct: 1   MAISLQLSRLSTRTDLAFPDGIGAFRS---RKPFSVRCAVDSSPS---SSSSVAVDSEFD 60

Query: 61  AKVFRRNLTKSDNYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYTWGDVTVKLAEA 120
           AKVFR+NLT+SDNYNRK FG K+ETL LMN EYTSD+IKTLKENG EYTWG+VTVKLAE+
Sbjct: 61  AKVFRKNLTRSDNYNRKRFGRKEETLELMNSEYTSDVIKTLKENGNEYTWGNVTVKLAES 120

Query: 121 YGFCWGVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPLEDGRKQFE 180
           YGFCWGVERAVQIAYEARKQFP+EKIWLTNEIIHNPTVNKRLEEM+VQ+IP+  G KQF+
Sbjct: 121 YGFCWGVERAVQIAYEARKQFPEEKIWLTNEIIHNPTVNKRLEEMKVQDIPINGGNKQFD 180

Query: 181 VVDKGDVVILPAFGAAVDEMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIHGK 240
           VV+KGDVVILPAFGAAVDEM  LS+K VQIVDTTCPWVSKVWN V+KHKKG+YTSIIHGK
Sbjct: 181 VVEKGDVVILPAFGAAVDEMRVLSDKNVQIVDTTCPWVSKVWNTVEKHKKGEYTSIIHGK 240

Query: 241 YAHEETVATASFAGTFIIVKNMAEAIYVCDYILGGELDGSSSTKEEFMKKFKNAVSKGFD 300
           YAHEETVATASFAG +IIVKNMAEAIYVCDYILGGELDGSSSTKEEFM+KFK A+SKGFD
Sbjct: 241 YAHEETVATASFAGKYIIVKNMAEAIYVCDYILGGELDGSSSTKEEFMEKFKFAMSKGFD 300

Query: 301 PDVDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQD 360
           PD DL K+GIANQTTMLKGETEEIGKL+ERTMMRK+GVENVNEHFLSFNTICDATQERQD
Sbjct: 301 PDTDLAKLGIANQTTMLKGETEEIGKLLERTMMRKYGVENVNEHFLSFNTICDATQERQD 360

Query: 361 AMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHG 420
           AMYKLV+ KLDLMLV+GGWNSSNTSHLQEIAEERGIPSYWIDSEQR+GPGN+I+YKLNHG
Sbjct: 361 AMYKLVDEKLDLMLVIGGWNSSNTSHLQEIAEERGIPSYWIDSEQRVGPGNKISYKLNHG 420

Query: 421 ELVEKENWLPKGHITIGVTSGASTPDKVVEDALIKVFDIKREEALQFA 469
           ELVEKENWLPKGH+TIGVTSGASTPDKVVED LI+VF+IKREEALQ A
Sbjct: 421 ELVEKENWLPKGHVTIGVTSGASTPDKVVEDVLIRVFEIKREEALQLA 462

BLAST of Cucsa.220370 vs. TrEMBL
Match: F6HQJ5_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_03s0063g02030 PE=3 SV=1)

HSP 1 Score: 800.0 bits (2065), Expect = 1.5e-228
Identity = 392/468 (83.76%), Postives = 431/468 (92.09%), Query Frame = 1

Query: 1   MAFSMQLYRLPTRTDAAMPENFTAVRSLRYRKPMSVRCDGDSSSSSSSSSSSAAVDSNFD 60
           MA S+QL R  T +D ++PE F  +   R RKP+SVRC G+S SS   SSSS AVDS+FD
Sbjct: 1   MAMSLQLCRFSTFSDRSLPEAFAGIGVFRRRKPLSVRCSGESESS---SSSSVAVDSDFD 60

Query: 61  AKVFRRNLTKSDNYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYTWGDVTVKLAEA 120
           AKVFR NLT+S NYNRKGFG+KDETL LMNREYTSDIIKTLKENG EY WG+VTVKLAEA
Sbjct: 61  AKVFRHNLTRSKNYNRKGFGHKDETLELMNREYTSDIIKTLKENGNEYKWGNVTVKLAEA 120

Query: 121 YGFCWGVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPLEDGRKQFE 180
           YGFCWGVERAVQIAYEARKQFP+EKIW+TNEIIHNPTVN+RL EMEV++IP++DG+KQFE
Sbjct: 121 YGFCWGVERAVQIAYEARKQFPEEKIWITNEIIHNPTVNQRLAEMEVKDIPIDDGQKQFE 180

Query: 181 VVDKGDVVILPAFGAAVDEMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIHGK 240
           VVDKGDVVILPAFGAAVDEMLTLS K VQIVDTTCPWVSKVWN+V+KHKKG+YTSIIHGK
Sbjct: 181 VVDKGDVVILPAFGAAVDEMLTLSNKNVQIVDTTCPWVSKVWNIVEKHKKGEYTSIIHGK 240

Query: 241 YAHEETVATASFAGTFIIVKNMAEAIYVCDYILGGELDGSSSTKEEFMKKFKNAVSKGFD 300
           Y+HEET+ATASFAG +IIVKNMAEA+YVCDYILGGELDGSSST+EEF +KFK A+S+GFD
Sbjct: 241 YSHEETIATASFAGKYIIVKNMAEAMYVCDYILGGELDGSSSTREEFFEKFKFAISEGFD 300

Query: 301 PDVDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQD 360
           PD+DL KVGIANQTTMLKGETEEIGKLVERTMMRK+GVENVN HF+SFNTICDATQERQD
Sbjct: 301 PDIDLSKVGIANQTTMLKGETEEIGKLVERTMMRKYGVENVNNHFISFNTICDATQERQD 360

Query: 361 AMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHG 420
           AMYKLVE KLD+MLVVGGWNSSNTSHLQEIAE+RGIPSYWIDSE+RIGPGNRI++KL HG
Sbjct: 361 AMYKLVEEKLDVMLVVGGWNSSNTSHLQEIAEDRGIPSYWIDSEKRIGPGNRISHKLMHG 420

Query: 421 ELVEKENWLPKGHITIGVTSGASTPDKVVEDALIKVFDIKREEALQFA 469
           ELVEKENWLP+G ITIGVTSGASTPDKVVED LIKVFDIKREEALQ A
Sbjct: 421 ELVEKENWLPEGPITIGVTSGASTPDKVVEDVLIKVFDIKREEALQLA 465

BLAST of Cucsa.220370 vs. TrEMBL
Match: A0A0M4K5E2_MORAL (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Morus alba PE=2 SV=1)

HSP 1 Score: 798.5 bits (2061), Expect = 4.4e-228
Identity = 390/468 (83.33%), Postives = 429/468 (91.67%), Query Frame = 1

Query: 1   MAFSMQLYRLPTRTDAAMPENFTAVRSLRYRKPMSVRCDGDSSSSSSSSSSSAAVDSNFD 60
           MA + QL R+PTRTD  +PE F    SLR RKP+S+RC GDSSS+SS +    A +S+FD
Sbjct: 1   MAIAFQLCRIPTRTDLFLPETF----SLRCRKPLSLRCSGDSSSASSPA---VAAESDFD 60

Query: 61  AKVFRRNLTKSDNYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYTWGDVTVKLAEA 120
           AKVFR+NLT+S NYNRKGFG+K+ETL LMNREYTSD+IKTLKENG EYTWG+VTVKLAEA
Sbjct: 61  AKVFRKNLTRSKNYNRKGFGHKEETLELMNREYTSDVIKTLKENGNEYTWGNVTVKLAEA 120

Query: 121 YGFCWGVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPLEDGRKQFE 180
           YGFCWGVERAVQIAYEARKQFPDEKIW+TNEIIHNPTVNKRLEEM+V+NIP+E G KQFE
Sbjct: 121 YGFCWGVERAVQIAYEARKQFPDEKIWITNEIIHNPTVNKRLEEMKVENIPIEQGNKQFE 180

Query: 181 VVDKGDVVILPAFGAAVDEMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIHGK 240
           VVDKGDVVILPAFGAAV+EMLTLS+K VQIVDTTCPWVSKVWN V+KHKKG+YTSIIHGK
Sbjct: 181 VVDKGDVVILPAFGAAVEEMLTLSDKNVQIVDTTCPWVSKVWNTVEKHKKGEYTSIIHGK 240

Query: 241 YAHEETVATASFAGTFIIVKNMAEAIYVCDYILGGELDGSSSTKEEFMKKFKNAVSKGFD 300
           Y+HEET+ATASFAGT+IIVKNM EA YVCDYILGG LDGSSST+E F++KFK AVS GFD
Sbjct: 241 YSHEETIATASFAGTYIIVKNMQEATYVCDYILGGALDGSSSTREAFLEKFKYAVSDGFD 300

Query: 301 PDVDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQD 360
           PD DL+KVGIANQTTMLKGETEEIGKL ERTMM+K+GVENVN+HF+SFNTICDATQERQD
Sbjct: 301 PDKDLVKVGIANQTTMLKGETEEIGKLAERTMMQKYGVENVNDHFISFNTICDATQERQD 360

Query: 361 AMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHG 420
           AMYK+VE  LDL+LVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHG
Sbjct: 361 AMYKMVEDDLDLILVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHG 420

Query: 421 ELVEKENWLPKGHITIGVTSGASTPDKVVEDALIKVFDIKREEALQFA 469
           ELVEKENWLPKG + IG+TSGASTPDKVVED LIKVFD+KREEALQ A
Sbjct: 421 ELVEKENWLPKGPLAIGITSGASTPDKVVEDVLIKVFDLKREEALQLA 461

BLAST of Cucsa.220370 vs. TAIR10
Match: AT4G34350.1 (AT4G34350.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase)

HSP 1 Score: 743.4 bits (1918), Expect = 8.4e-215
Identity = 363/469 (77.40%), Postives = 419/469 (89.34%), Query Frame = 1

Query: 1   MAFSMQLYRLPTRTDAAMPEN-FTAVRSLRYRKPMSVRCDGDSSSSSSSSSSSAAVDSNF 60
           MA ++Q  RL  R D  + EN  +   SLR RK +SVRC   SS   ++ S S  +DS+F
Sbjct: 1   MAVALQFSRLCVRPDTFVRENHLSGSGSLRRRKALSVRC---SSGDENAPSPSVVMDSDF 60

Query: 61  DAKVFRRNLTKSDNYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYTWGDVTVKLAE 120
           DAKVFR+NLT+SDNYNRKGFG+K+ETL LMNREYTSDI++TLK NG+ Y+WGDVTVKLA+
Sbjct: 61  DAKVFRKNLTRSDNYNRKGFGHKEETLKLMNREYTSDILETLKTNGYTYSWGDVTVKLAK 120

Query: 121 AYGFCWGVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPLEDGRKQF 180
           AYGFCWGVERAVQIAYEARKQFP+E++W+TNEIIHNPTVNKRLE+M+V+ IP+ED +KQF
Sbjct: 121 AYGFCWGVERAVQIAYEARKQFPEERLWITNEIIHNPTVNKRLEDMDVKIIPVEDSKKQF 180

Query: 181 EVVDKGDVVILPAFGAAVDEMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIHG 240
           +VV+K DVVILPAFGA VDEM  L++KKVQIVDTTCPWV+KVWN V+KHKKG+YTS+IHG
Sbjct: 181 DVVEKDDVVILPAFGAGVDEMYVLNDKKVQIVDTTCPWVTKVWNTVEKHKKGEYTSVIHG 240

Query: 241 KYAHEETVATASFAGTFIIVKNMAEAIYVCDYILGGELDGSSSTKEEFMKKFKNAVSKGF 300
           KY HEET+ATASFAG +IIVKNM EA YVCDYILGG+ DGSSSTKEEFM+KFK A+SKGF
Sbjct: 241 KYNHEETIATASFAGKYIIVKNMKEANYVCDYILGGQYDGSSSTKEEFMEKFKYAISKGF 300

Query: 301 DPDVDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQ 360
           DPD DL+KVGIANQTTMLKGETEEIG+L+E TMMRK+GVENV+ HF+SFNTICDATQERQ
Sbjct: 301 DPDNDLVKVGIANQTTMLKGETEEIGRLLETTMMRKYGVENVSGHFISFNTICDATQERQ 360

Query: 361 DAMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNH 420
           DA+Y+LVE K+DLMLVVGGWNSSNTSHLQEI+E RGIPSYWIDSE+RIGPGN+IAYKL++
Sbjct: 361 DAIYELVEEKIDLMLVVGGWNSSNTSHLQEISEARGIPSYWIDSEKRIGPGNKIAYKLHY 420

Query: 421 GELVEKENWLPKGHITIGVTSGASTPDKVVEDALIKVFDIKREEALQFA 469
           GELVEKEN+LPKG ITIGVTSGASTPDKVVEDAL+KVFDIKREE LQ A
Sbjct: 421 GELVEKENFLPKGPITIGVTSGASTPDKVVEDALVKVFDIKREELLQLA 466

BLAST of Cucsa.220370 vs. NCBI nr
Match: gi|778725300|ref|XP_004137008.2| (PREDICTED: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic [Cucumis sativus])

HSP 1 Score: 941.4 bits (2432), Expect = 6.0e-271
Identity = 468/468 (100.00%), Postives = 468/468 (100.00%), Query Frame = 1

Query: 1   MAFSMQLYRLPTRTDAAMPENFTAVRSLRYRKPMSVRCDGDSSSSSSSSSSSAAVDSNFD 60
           MAFSMQLYRLPTRTDAAMPENFTAVRSLRYRKPMSVRCDGDSSSSSSSSSSSAAVDSNFD
Sbjct: 1   MAFSMQLYRLPTRTDAAMPENFTAVRSLRYRKPMSVRCDGDSSSSSSSSSSSAAVDSNFD 60

Query: 61  AKVFRRNLTKSDNYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYTWGDVTVKLAEA 120
           AKVFRRNLTKSDNYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYTWGDVTVKLAEA
Sbjct: 61  AKVFRRNLTKSDNYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYTWGDVTVKLAEA 120

Query: 121 YGFCWGVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPLEDGRKQFE 180
           YGFCWGVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPLEDGRKQFE
Sbjct: 121 YGFCWGVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPLEDGRKQFE 180

Query: 181 VVDKGDVVILPAFGAAVDEMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIHGK 240
           VVDKGDVVILPAFGAAVDEMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIHGK
Sbjct: 181 VVDKGDVVILPAFGAAVDEMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIHGK 240

Query: 241 YAHEETVATASFAGTFIIVKNMAEAIYVCDYILGGELDGSSSTKEEFMKKFKNAVSKGFD 300
           YAHEETVATASFAGTFIIVKNMAEAIYVCDYILGGELDGSSSTKEEFMKKFKNAVSKGFD
Sbjct: 241 YAHEETVATASFAGTFIIVKNMAEAIYVCDYILGGELDGSSSTKEEFMKKFKNAVSKGFD 300

Query: 301 PDVDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQD 360
           PDVDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQD
Sbjct: 301 PDVDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQD 360

Query: 361 AMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHG 420
           AMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHG
Sbjct: 361 AMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHG 420

Query: 421 ELVEKENWLPKGHITIGVTSGASTPDKVVEDALIKVFDIKREEALQFA 469
           ELVEKENWLPKGHITIGVTSGASTPDKVVEDALIKVFDIKREEALQFA
Sbjct: 421 ELVEKENWLPKGHITIGVTSGASTPDKVVEDALIKVFDIKREEALQFA 468

BLAST of Cucsa.220370 vs. NCBI nr
Match: gi|659109783|ref|XP_008454877.1| (PREDICTED: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like [Cucumis melo])

HSP 1 Score: 912.1 bits (2356), Expect = 3.9e-262
Identity = 454/468 (97.01%), Postives = 461/468 (98.50%), Query Frame = 1

Query: 1   MAFSMQLYRLPTRTDAAMPENFTAVRSLRYRKPMSVRCDGDSSSSSSSSSSSAAVDSNFD 60
           MAFSMQLYRLPTRTDAA+PEN +AVRSLRYRKPMSVRCDGD     SSSSSSAAVDS+FD
Sbjct: 1   MAFSMQLYRLPTRTDAALPENSSAVRSLRYRKPMSVRCDGD-----SSSSSSAAVDSDFD 60

Query: 61  AKVFRRNLTKSDNYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYTWGDVTVKLAEA 120
           AKVFRRNLTKSDNYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYTWGDVTVKLAEA
Sbjct: 61  AKVFRRNLTKSDNYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYTWGDVTVKLAEA 120

Query: 121 YGFCWGVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPLEDGRKQFE 180
           YGFCWGVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPLE+GRKQFE
Sbjct: 121 YGFCWGVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPLEEGRKQFE 180

Query: 181 VVDKGDVVILPAFGAAVDEMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIHGK 240
           VVDKGDVVILPAFGAAVDEMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIHGK
Sbjct: 181 VVDKGDVVILPAFGAAVDEMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIHGK 240

Query: 241 YAHEETVATASFAGTFIIVKNMAEAIYVCDYILGGELDGSSSTKEEFMKKFKNAVSKGFD 300
           YAHEETVATASFAG +IIVKNMAEA+YVCDYILGGELDGSSSTKEEFMKKFKNAVS+GFD
Sbjct: 241 YAHEETVATASFAGKYIIVKNMAEAMYVCDYILGGELDGSSSTKEEFMKKFKNAVSEGFD 300

Query: 301 PDVDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQD 360
           PDVDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQD
Sbjct: 301 PDVDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQD 360

Query: 361 AMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHG 420
           AMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHG
Sbjct: 361 AMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHG 420

Query: 421 ELVEKENWLPKGHITIGVTSGASTPDKVVEDALIKVFDIKREEALQFA 469
           ELVEKENWLPKGHITIGVTSGASTPDKVVEDALIKVFDIKREEALQFA
Sbjct: 421 ELVEKENWLPKGHITIGVTSGASTPDKVVEDALIKVFDIKREEALQFA 463

BLAST of Cucsa.220370 vs. NCBI nr
Match: gi|343466165|gb|AEM42976.1| (4-hydroxy-3-methylbut-2-enyl diphosphate reductase [Siraitia grosvenorii])

HSP 1 Score: 880.2 bits (2273), Expect = 1.6e-252
Identity = 429/470 (91.28%), Postives = 459/470 (97.66%), Query Frame = 1

Query: 1   MAFSMQLYRLPTRTDAAMPENFTAVRSLRYRKPMSVRCDGDSSSSSSSSSSSAAV--DSN 60
           MAFS+QLYRL TRTDAA+PENF+ +R++RYRKP+SVRCDGDSSSSSSSSSSS++V  DSN
Sbjct: 1   MAFSLQLYRLSTRTDAALPENFSGIRAVRYRKPLSVRCDGDSSSSSSSSSSSSSVAMDSN 60

Query: 61  FDAKVFRRNLTKSDNYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYTWGDVTVKLA 120
           FDAKVFR+NLT+S+NYNRKGFGYKDETLALMNREYTSDIIKTLKE+ +EYTWGDVTVKLA
Sbjct: 61  FDAKVFRKNLTRSENYNRKGFGYKDETLALMNREYTSDIIKTLKEHEYEYTWGDVTVKLA 120

Query: 121 EAYGFCWGVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPLEDGRKQ 180
           EAYGFCWGVERAVQIAYEARKQFPDEKIW+TNEIIHNPTVNKRLEEMEV+NIPLE+G+KQ
Sbjct: 121 EAYGFCWGVERAVQIAYEARKQFPDEKIWITNEIIHNPTVNKRLEEMEVENIPLEEGKKQ 180

Query: 181 FEVVDKGDVVILPAFGAAVDEMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIH 240
           FEVV+KGDVVILPAFGAAVDEMLTLSEK VQIVDTTCPWVSKVWNVV+KHKKGD+TSIIH
Sbjct: 181 FEVVNKGDVVILPAFGAAVDEMLTLSEKNVQIVDTTCPWVSKVWNVVEKHKKGDFTSIIH 240

Query: 241 GKYAHEETVATASFAGTFIIVKNMAEAIYVCDYILGGELDGSSSTKEEFMKKFKNAVSKG 300
           GKYAHEETVATASFAG +IIVKNMAEA YVCDYILGGELDGSS TKEEFMKKFKNAVSKG
Sbjct: 241 GKYAHEETVATASFAGKYIIVKNMAEATYVCDYILGGELDGSSPTKEEFMKKFKNAVSKG 300

Query: 301 FDPDVDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQER 360
           FDPD+DL+KVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHF+SFNTICDATQER
Sbjct: 301 FDPDIDLVKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFISFNTICDATQER 360

Query: 361 QDAMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLN 420
           QDAMYKLVEGKLDLMLVVGGWNSSNTSHLQ+IAE+RGIPSYW+DSEQRIGPGN+IAYKLN
Sbjct: 361 QDAMYKLVEGKLDLMLVVGGWNSSNTSHLQQIAEDRGIPSYWVDSEQRIGPGNKIAYKLN 420

Query: 421 HGELVEKENWLPKGHITIGVTSGASTPDKVVEDALIKVFDIKREEALQFA 469
           HGELVEKENWLP+G ITIGVTSGASTPDKVVED LIKVFDIKREEALQFA
Sbjct: 421 HGELVEKENWLPRGPITIGVTSGASTPDKVVEDVLIKVFDIKREEALQFA 470

BLAST of Cucsa.220370 vs. NCBI nr
Match: gi|826136647|gb|AKJ26298.1| (hydroxymethylbutenyl diphosphate reductase [Paeonia lactiflora])

HSP 1 Score: 800.8 bits (2067), Expect = 1.3e-228
Identity = 397/468 (84.83%), Postives = 428/468 (91.45%), Query Frame = 1

Query: 1   MAFSMQLYRLPTRTDAAMPENFTAVRSLRYRKPMSVRCDGDSSSSSSSSSSSAAVDSNFD 60
           MA S+QL RL TRTD A P+   A RS   RKP SVRC  DSS S   SSSS AVDS FD
Sbjct: 1   MAISLQLSRLSTRTDLAFPDGIGAFRS---RKPFSVRCAVDSSPS---SSSSVAVDSEFD 60

Query: 61  AKVFRRNLTKSDNYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYTWGDVTVKLAEA 120
           AKVFR+NLT+SDNYNRK FG K+ETL LMN EYTSD+IKTLKENG EYTWG+VTVKLAE+
Sbjct: 61  AKVFRKNLTRSDNYNRKRFGRKEETLELMNSEYTSDVIKTLKENGNEYTWGNVTVKLAES 120

Query: 121 YGFCWGVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPLEDGRKQFE 180
           YGFCWGVERAVQIAYEARKQFP+EKIWLTNEIIHNPTVNKRLEEM+VQ+IP+  G KQF+
Sbjct: 121 YGFCWGVERAVQIAYEARKQFPEEKIWLTNEIIHNPTVNKRLEEMKVQDIPINGGNKQFD 180

Query: 181 VVDKGDVVILPAFGAAVDEMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIHGK 240
           VV+KGDVVILPAFGAAVDEM  LS+K VQIVDTTCPWVSKVWN V+KHKKG+YTSIIHGK
Sbjct: 181 VVEKGDVVILPAFGAAVDEMRVLSDKNVQIVDTTCPWVSKVWNTVEKHKKGEYTSIIHGK 240

Query: 241 YAHEETVATASFAGTFIIVKNMAEAIYVCDYILGGELDGSSSTKEEFMKKFKNAVSKGFD 300
           YAHEETVATASFAG +IIVKNMAEAIYVCDYILGGELDGSSSTKEEFM+KFK A+SKGFD
Sbjct: 241 YAHEETVATASFAGKYIIVKNMAEAIYVCDYILGGELDGSSSTKEEFMEKFKFAMSKGFD 300

Query: 301 PDVDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQD 360
           PD DL K+GIANQTTMLKGETEEIGKL+ERTMMRK+GVENVNEHFLSFNTICDATQERQD
Sbjct: 301 PDTDLAKLGIANQTTMLKGETEEIGKLLERTMMRKYGVENVNEHFLSFNTICDATQERQD 360

Query: 361 AMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHG 420
           AMYKLV+ KLDLMLV+GGWNSSNTSHLQEIAEERGIPSYWIDSEQR+GPGN+I+YKLNHG
Sbjct: 361 AMYKLVDEKLDLMLVIGGWNSSNTSHLQEIAEERGIPSYWIDSEQRVGPGNKISYKLNHG 420

Query: 421 ELVEKENWLPKGHITIGVTSGASTPDKVVEDALIKVFDIKREEALQFA 469
           ELVEKENWLPKGH+TIGVTSGASTPDKVVED LI+VF+IKREEALQ A
Sbjct: 421 ELVEKENWLPKGHVTIGVTSGASTPDKVVEDVLIRVFEIKREEALQLA 462

BLAST of Cucsa.220370 vs. NCBI nr
Match: gi|225428566|ref|XP_002284659.1| (PREDICTED: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic [Vitis vinifera])

HSP 1 Score: 800.0 bits (2065), Expect = 2.1e-228
Identity = 392/468 (83.76%), Postives = 431/468 (92.09%), Query Frame = 1

Query: 1   MAFSMQLYRLPTRTDAAMPENFTAVRSLRYRKPMSVRCDGDSSSSSSSSSSSAAVDSNFD 60
           MA S+QL R  T +D ++PE F  +   R RKP+SVRC G+S SS   SSSS AVDS+FD
Sbjct: 1   MAMSLQLCRFSTFSDRSLPEAFAGIGVFRRRKPLSVRCSGESESS---SSSSVAVDSDFD 60

Query: 61  AKVFRRNLTKSDNYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYTWGDVTVKLAEA 120
           AKVFR NLT+S NYNRKGFG+KDETL LMNREYTSDIIKTLKENG EY WG+VTVKLAEA
Sbjct: 61  AKVFRHNLTRSKNYNRKGFGHKDETLELMNREYTSDIIKTLKENGNEYKWGNVTVKLAEA 120

Query: 121 YGFCWGVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPLEDGRKQFE 180
           YGFCWGVERAVQIAYEARKQFP+EKIW+TNEIIHNPTVN+RL EMEV++IP++DG+KQFE
Sbjct: 121 YGFCWGVERAVQIAYEARKQFPEEKIWITNEIIHNPTVNQRLAEMEVKDIPIDDGQKQFE 180

Query: 181 VVDKGDVVILPAFGAAVDEMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIHGK 240
           VVDKGDVVILPAFGAAVDEMLTLS K VQIVDTTCPWVSKVWN+V+KHKKG+YTSIIHGK
Sbjct: 181 VVDKGDVVILPAFGAAVDEMLTLSNKNVQIVDTTCPWVSKVWNIVEKHKKGEYTSIIHGK 240

Query: 241 YAHEETVATASFAGTFIIVKNMAEAIYVCDYILGGELDGSSSTKEEFMKKFKNAVSKGFD 300
           Y+HEET+ATASFAG +IIVKNMAEA+YVCDYILGGELDGSSST+EEF +KFK A+S+GFD
Sbjct: 241 YSHEETIATASFAGKYIIVKNMAEAMYVCDYILGGELDGSSSTREEFFEKFKFAISEGFD 300

Query: 301 PDVDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQD 360
           PD+DL KVGIANQTTMLKGETEEIGKLVERTMMRK+GVENVN HF+SFNTICDATQERQD
Sbjct: 301 PDIDLSKVGIANQTTMLKGETEEIGKLVERTMMRKYGVENVNNHFISFNTICDATQERQD 360

Query: 361 AMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHG 420
           AMYKLVE KLD+MLVVGGWNSSNTSHLQEIAE+RGIPSYWIDSE+RIGPGNRI++KL HG
Sbjct: 361 AMYKLVEEKLDVMLVVGGWNSSNTSHLQEIAEDRGIPSYWIDSEKRIGPGNRISHKLMHG 420

Query: 421 ELVEKENWLPKGHITIGVTSGASTPDKVVEDALIKVFDIKREEALQFA 469
           ELVEKENWLP+G ITIGVTSGASTPDKVVED LIKVFDIKREEALQ A
Sbjct: 421 ELVEKENWLPEGPITIGVTSGASTPDKVVEDVLIKVFDIKREEALQLA 465